LOCUS       DAMJYX010000001       105538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJYX010000001 DAMJYX010000000
VERSION     DAMJYX010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 105538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.001"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..159)
                     /locus_tag="PC479_000001"
     CDS             complement(<1..159)
                     /locus_tag="PC479_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802592.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDN"
     gene            complement(174..617)
                     /locus_tag="PC479_000002"
     CDS             complement(174..617)
                     /locus_tag="PC479_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802593.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            complement(618..1490)
                     /locus_tag="PC479_000003"
     CDS             complement(618..1490)
                     /locus_tag="PC479_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2802594.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            complement(1543..1902)
                     /locus_tag="PC479_000004"
     CDS             complement(1543..1902)
                     /locus_tag="PC479_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2802595.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(1956..3041)
                     /locus_tag="PC479_000005"
     CDS             complement(1956..3041)
                     /locus_tag="PC479_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2802596.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(3212..4591)
                     /locus_tag="PC479_000006"
     CDS             complement(3212..4591)
                     /locus_tag="PC479_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2802597.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(4584..5264)
                     /locus_tag="PC479_000007"
     CDS             complement(4584..5264)
                     /locus_tag="PC479_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2802598.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(5655..6734)
                     /locus_tag="PC479_000008"
     CDS             complement(5655..6734)
                     /locus_tag="PC479_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2802599.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      7022..7137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7312..8682
                     /locus_tag="PC479_000009"
     CDS             7312..8682
                     /locus_tag="PC479_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2802600.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8947..9558)
                     /locus_tag="PC479_000010"
     CDS             complement(8947..9558)
                     /locus_tag="PC479_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2802601.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(9548..11911)
                     /gene="lon"
                     /locus_tag="PC479_000011"
     CDS             complement(9548..11911)
                     /gene="lon"
                     /locus_tag="PC479_000011"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2802602.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            12091..12603
                     /locus_tag="PC479_000012"
     CDS             12091..12603
                     /locus_tag="PC479_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2802603.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            12591..13118
                     /locus_tag="PC479_000013"
     CDS             12591..13118
                     /locus_tag="PC479_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2802604.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(13189..14439)
                     /gene="clpX"
                     /locus_tag="PC479_000014"
     CDS             complement(13189..14439)
                     /gene="clpX"
                     /locus_tag="PC479_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2802605.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(14458..15042)
                     /gene="clpP"
                     /locus_tag="PC479_000015"
     CDS             complement(14458..15042)
                     /gene="clpP"
                     /locus_tag="PC479_000015"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2802606.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(15192..16478)
                     /locus_tag="PC479_000016"
     CDS             complement(15192..16478)
                     /locus_tag="PC479_000016"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2802607.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(16672..17142)
                     /locus_tag="PC479_000017"
     CDS             complement(16672..17142)
                     /locus_tag="PC479_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2802608.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(17142..18491)
                     /gene="rph"
                     /locus_tag="PC479_000018"
     CDS             complement(17142..18491)
                     /gene="rph"
                     /locus_tag="PC479_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2802609.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(18651..20675)
                     /gene="ligA"
                     /locus_tag="PC479_000019"
     CDS             complement(18651..20675)
                     /gene="ligA"
                     /locus_tag="PC479_000019"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2802610.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20861..21799)
                     /locus_tag="PC479_000020"
     CDS             complement(20861..21799)
                     /locus_tag="PC479_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2802611.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21901..22323)
                     /locus_tag="PC479_000021"
     CDS             complement(21901..22323)
                     /locus_tag="PC479_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2802612.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            22511..23794
                     /locus_tag="PC479_000022"
     CDS             22511..23794
                     /locus_tag="PC479_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2802613.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23872..24387)
                     /locus_tag="PC479_000023"
     CDS             complement(23872..24387)
                     /locus_tag="PC479_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2802614.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(24387..25763)
                     /locus_tag="PC479_000024"
     CDS             complement(24387..25763)
                     /locus_tag="PC479_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2802615.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25788..26339)
                     /locus_tag="PC479_000025"
     CDS             complement(25788..26339)
                     /locus_tag="PC479_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2802616.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(26397..26612)
                     /locus_tag="PC479_000026"
     CDS             complement(26397..26612)
                     /locus_tag="PC479_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802617.1"
                     /translation="MQIHRELSKSENEYMCTKKEEQRYSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(26581..27414)
                     /gene="fdhD"
                     /locus_tag="PC479_000027"
     CDS             complement(26581..27414)
                     /gene="fdhD"
                     /locus_tag="PC479_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2802618.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(27463..29607)
                     /locus_tag="PC479_000028"
     CDS             complement(27463..29607)
                     /locus_tag="PC479_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(29191..29193),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF2802619.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29858..30916)
                     /locus_tag="PC479_000029"
     CDS             complement(29858..30916)
                     /locus_tag="PC479_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2802620.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(31127..31786)
                     /gene="cprR"
                     /locus_tag="PC479_000030"
     CDS             complement(31127..31786)
                     /gene="cprR"
                     /locus_tag="PC479_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2802621.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            32096..32725
                     /locus_tag="PC479_000031"
     CDS             32096..32725
                     /locus_tag="PC479_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2802622.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            32793..33626
                     /locus_tag="PC479_000032"
     CDS             32793..33626
                     /locus_tag="PC479_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2802623.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(33722..34510)
                     /locus_tag="PC479_000033"
     CDS             complement(33722..34510)
                     /locus_tag="PC479_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802624.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(34511..35383)
                     /locus_tag="PC479_000034"
     CDS             complement(34511..35383)
                     /locus_tag="PC479_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2802625.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            complement(35398..37107)
                     /locus_tag="PC479_000035"
     CDS             complement(35398..37107)
                     /locus_tag="PC479_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802626.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            complement(37254..37835)
                     /locus_tag="PC479_000036"
     CDS             complement(37254..37835)
                     /locus_tag="PC479_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2802627.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            complement(37894..38034)
                     /locus_tag="PC479_000037"
     CDS             complement(37894..38034)
                     /locus_tag="PC479_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2802628.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            complement(38046..39362)
                     /locus_tag="PC479_000038"
     CDS             complement(38046..39362)
                     /locus_tag="PC479_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2802629.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            complement(39356..40066)
                     /locus_tag="PC479_000039"
     CDS             complement(39356..40066)
                     /locus_tag="PC479_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2802630.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            complement(40505..41080)
                     /locus_tag="PC479_000040"
     CDS             complement(40505..41080)
                     /locus_tag="PC479_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2802631.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(41373..41651)
                     /locus_tag="PC479_000041"
     CDS             complement(41373..41651)
                     /locus_tag="PC479_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802632.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            complement(41960..42721)
                     /locus_tag="PC479_000042"
     CDS             complement(41960..42721)
                     /locus_tag="PC479_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2802633.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            complement(42890..44965)
                     /gene="bclA3"
                     /locus_tag="PC479_000043"
     CDS             complement(42890..44965)
                     /gene="bclA3"
                     /locus_tag="PC479_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2802634.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            complement(45032..46108)
                     /locus_tag="PC479_000044"
     CDS             complement(45032..46108)
                     /locus_tag="PC479_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2802635.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            complement(46420..47004)
                     /gene="clpP"
                     /locus_tag="PC479_000045"
     CDS             complement(46420..47004)
                     /gene="clpP"
                     /locus_tag="PC479_000045"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2802636.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            complement(47001..47459)
                     /locus_tag="PC479_000046"
     CDS             complement(47001..47459)
                     /locus_tag="PC479_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2802637.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            complement(47682..49097)
                     /locus_tag="PC479_000047"
     CDS             complement(47682..49097)
                     /locus_tag="PC479_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2802638.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            49212..49880
                     /locus_tag="PC479_000048"
     CDS             49212..49880
                     /locus_tag="PC479_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802639.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            complement(50056..50574)
                     /locus_tag="PC479_000049"
     CDS             complement(50056..50574)
                     /locus_tag="PC479_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2802640.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            50726..51049
                     /locus_tag="PC479_000050"
     CDS             50726..51049
                     /locus_tag="PC479_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2802641.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            complement(51054..51638)
                     /locus_tag="PC479_000051"
     CDS             complement(51054..51638)
                     /locus_tag="PC479_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2802642.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(51687..52142)
                     /locus_tag="PC479_000052"
     CDS             complement(51687..52142)
                     /locus_tag="PC479_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2802643.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            53024..54562
                     /locus_tag="PC479_000053"
     CDS             53024..54562
                     /locus_tag="PC479_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2802644.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            complement(54834..55547)
                     /locus_tag="PC479_000054"
     CDS             complement(54834..55547)
                     /locus_tag="PC479_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2802645.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(55995..57374)
                     /locus_tag="PC479_000055"
     CDS             complement(55995..57374)
                     /locus_tag="PC479_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2802646.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            complement(57659..58453)
                     /locus_tag="PC479_000056"
     CDS             complement(57659..58453)
                     /locus_tag="PC479_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2802647.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            complement(58450..59313)
                     /locus_tag="PC479_000057"
     CDS             complement(58450..59313)
                     /locus_tag="PC479_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2802648.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            complement(59291..60286)
                     /locus_tag="PC479_000058"
     CDS             complement(59291..60286)
                     /locus_tag="PC479_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2802649.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            60989..61945
                     /locus_tag="PC479_000059"
     CDS             60989..61945
                     /locus_tag="PC479_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2802650.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(62338..63228)
                     /locus_tag="PC479_000060"
     CDS             complement(62338..63228)
                     /locus_tag="PC479_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802651.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(63406..64104)
                     /locus_tag="PC479_000061"
     CDS             complement(63406..64104)
                     /locus_tag="PC479_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802652.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(64640..65707)
                     /gene="rsgA"
                     /locus_tag="PC479_000062"
     CDS             complement(64640..65707)
                     /gene="rsgA"
                     /locus_tag="PC479_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2802653.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(65704..66105)
                     /locus_tag="PC479_000063"
     CDS             complement(65704..66105)
                     /locus_tag="PC479_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2802654.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      66902..66998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            67052..67228
                     /locus_tag="PC479_000064"
     CDS             67052..67228
                     /locus_tag="PC479_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802655.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            67638..68276
                     /locus_tag="PC479_000065"
     CDS             67638..68276
                     /locus_tag="PC479_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802656.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(68749..70107)
                     /gene="rlmD"
                     /locus_tag="PC479_000066"
     CDS             complement(68749..70107)
                     /gene="rlmD"
                     /locus_tag="PC479_000066"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2802657.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(70282..72042)
                     /gene="pyk"
                     /locus_tag="PC479_000067"
     CDS             complement(70282..72042)
                     /gene="pyk"
                     /locus_tag="PC479_000067"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2802658.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(72094..73053)
                     /gene="pfkA"
                     /locus_tag="PC479_000068"
     CDS             complement(72094..73053)
                     /gene="pfkA"
                     /locus_tag="PC479_000068"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2802659.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(73262..76831)
                     /locus_tag="PC479_000069"
     CDS             complement(73262..76831)
                     /locus_tag="PC479_000069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2802660.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(76988..77935)
                     /gene="whiA"
                     /locus_tag="PC479_000070"
     CDS             complement(76988..77935)
                     /gene="whiA"
                     /locus_tag="PC479_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2802661.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(77952..78377)
                     /locus_tag="PC479_000071"
     CDS             complement(77952..78377)
                     /locus_tag="PC479_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2802662.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(78446..79552)
                     /locus_tag="PC479_000072"
     CDS             complement(78446..79552)
                     /locus_tag="PC479_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2802663.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(79564..80421)
                     /gene="rapZ"
                     /locus_tag="PC479_000073"
     CDS             complement(79564..80421)
                     /gene="rapZ"
                     /locus_tag="PC479_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2802664.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(80452..81165)
                     /locus_tag="PC479_000074"
     CDS             complement(80452..81165)
                     /locus_tag="PC479_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2802665.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(81169..82083)
                     /gene="murB"
                     /locus_tag="PC479_000075"
     CDS             complement(81169..82083)
                     /gene="murB"
                     /locus_tag="PC479_000075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2802666.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(82356..83126)
                     /locus_tag="PC479_000076"
     CDS             complement(82356..83126)
                     /locus_tag="PC479_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2802667.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            83444..84925
                     /gene="cls"
                     /locus_tag="PC479_000077"
     CDS             83444..84925
                     /gene="cls"
                     /locus_tag="PC479_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2802668.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            85243..85740
                     /locus_tag="PC479_000078"
     CDS             85243..85740
                     /locus_tag="PC479_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2802669.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            85757..87643
                     /locus_tag="PC479_000079"
     CDS             85757..87643
                     /locus_tag="PC479_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2802670.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            87666..89447
                     /locus_tag="PC479_000080"
     CDS             87666..89447
                     /locus_tag="PC479_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2802671.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(89587..91383)
                     /locus_tag="PC479_000081"
     CDS             complement(89587..91383)
                     /locus_tag="PC479_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2802672.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(91595..92524)
                     /locus_tag="PC479_000082"
     CDS             complement(91595..92524)
                     /locus_tag="PC479_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2802673.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(92524..94341)
                     /gene="uvrC"
                     /locus_tag="PC479_000083"
     CDS             complement(92524..94341)
                     /gene="uvrC"
                     /locus_tag="PC479_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2802674.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(94600..97425)
                     /gene="uvrA"
                     /locus_tag="PC479_000084"
     CDS             complement(94600..97425)
                     /gene="uvrA"
                     /locus_tag="PC479_000084"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2802675.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(97444..99414)
                     /gene="uvrB"
                     /locus_tag="PC479_000085"
     CDS             complement(97444..99414)
                     /gene="uvrB"
                     /locus_tag="PC479_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2802676.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDNYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(99601..100863)
                     /locus_tag="PC479_000086"
     CDS             complement(99601..100863)
                     /locus_tag="PC479_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2802677.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(100926..102230)
                     /locus_tag="PC479_000087"
     CDS             complement(100926..102230)
                     /locus_tag="PC479_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2802678.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(102261..103121)
                     /locus_tag="PC479_000088"
     CDS             complement(102261..103121)
                     /locus_tag="PC479_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2802679.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(103165..104034)
                     /locus_tag="PC479_000089"
     CDS             complement(103165..104034)
                     /locus_tag="PC479_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2802680.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(104018..105088)
                     /locus_tag="PC479_000090"
     CDS             complement(104018..105088)
                     /locus_tag="PC479_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2802681.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        33796 a        18157 c        12161 g        41424 t
ORIGIN      
        1 attatcttga ttatttattt tatttattat attttgcttt tcattcttca aactagttag
       61 gttttcttcc aaataactta ctgctatatc acttgccaat ataataacta atattaacaa
      121 tattttaagt agattttttt ctctattatt taatctcaaa aataatcacc catctaactt
      181 tttattctaa atctataata ttctccatct cttacaatac tgtctatgta ataatatttt
      241 atgtacttta ttttccttaa attgtttaaa acatctaaat cactacacac accttccatc
      301 tctagcttat ttttatctat tgatatttta gtaatactat ttattccaat tgtttcatat
      361 atttttttta tttcattaag ttttatttta gaaaatttct ctctagtttt atctgttgtt
      421 ggtaaagtgt tctttgattt atccactagc ttattttgac ttatataatc ttcaagttca
      481 tttatagatt tcacatacat aaatgattta aataaaatac acgttaaaat taagactatt
      541 attattttat agattttatt acatggttta gtttttttaa atccattaag aaaatttatt
      601 gttttatcca ctttcactta aataatcatt cctatactat ttatataatt tatcctattg
      661 tcttctaaat ctgcatcaaa attattttct acttttactt tatcttttaa tcttaatgga
      721 tttacttcaa tagatttatc tgtatattta aagtattctt caataaaaca attttctata
      781 ccatagtaat aaacatactt aaaacccctt aataatcttt ttatgaactc atatgatttt
      841 ccctcttttg atagcaagta tgtttctctt atttttttat cttttgttat atttataatt
      901 aactgacttt ttttacattc tataaatacc ccttcttctg aaaaattctc tattaaattt
      961 aagtttataa gcttttgtaa tatatcaaaa tttatattta tagtctttgg attcatattt
     1021 agattttctg aaatgttctt aattaattga ataatattct ttgggaaaag aattacttgt
     1081 atattcaatt tatcattttt agaatttata actttatatt ttattgaata gttatttatg
     1141 tttattggaa tgtattgtgt tatttcaaat tcaatcatgc ttaatatgtc tctttttttc
     1201 ttagtattta gaacttcaac atttctaatg actatatcct gaatttgaat gttaaagtat
     1261 aaatttttat ataatatttt tgatttacca atctcctctt ttacaaatct aactacatct
     1321 tctatatatt taatcttctc aatcgaatta ctatcatttt ttatatcaaa gttaatctct
     1381 tttataattg atttatctat aaaaactttt ttatgacttt ttcttaaaaa tacaagcctt
     1441 atataatcat catttatttc taagtttaga atttttttaa ttatagccat ctccttctta
     1501 aataaaaata attttaaaac ttacagcaat caatataaaa tattattttg gataaaattg
     1561 cttagaattt atatttacgc ttatatctgt tccgctatca tttatgacaa gttcgaattt
     1621 acctgatgta ctctgaggaa ctggaacagt atttaggtat cccttttcct tcaatgtctc
     1681 tacacttaga gagtctattg tcttttcatc atcattaatt gccatgctag ctgcggttac
     1741 tatattagca gcattcgtat agtctgcatt taatttggca ctttcttgaa ccttactata
     1801 cttaactaat gctatactag atataacgcc taaaatagtt actactacaa tcatttcaac
     1861 aagtgtaaat ccttttcgtt ttttatttat caaatttatc aattaaatat tctctccttt
     1921 tcaaaagttt catctaataa aatttatttt tctctctaaa aattagtaat tgcatcaaac
     1981 ataggaacaa ccattgctaa aataaaagta ccaataatta atcccataaa tacaattagc
     2041 ataggttcta taaatttcat tatttgctct attttgttat ttaattcatt ttcataaaat
     2101 ttatttatta catttagact tttatctagt ttcccacttt cttctcctgt atttgccatt
     2161 gaaataaata attttggaaa aatatttgct aaattcaaag atttacctat gctatttcct
     2221 ttttttatat aattattaga aatctttaac ctttcataaa taaattcgtt gtctataaca
     2281 ctagctgaca tatctatagc ttccacaatt tgtattccac tgctaattaa aacggacaat
     2341 gcccttgaaa atcttgttgt agctactaac tgattaattt tttttataaa aggtattttg
     2401 aattttaacg aattaatcaa tttttttata ttatcatttg ttataaagaa ataaatgctt
     2461 cctattgaaa acaatataaa actaaatatt atataagcta agttatttgt aaccactgta
     2521 gatattccca ttaaaactct agttattaaa ggtggttcaa ttccattgtt ggtaaaaacc
     2581 acctgaaaat taggtattac aaatacgaat ataaacaaca ttgataagat agataatatt
     2641 ataagtatta ttggatatat tgaaatagtt attattttca tttttaattt gtattcttta
     2701 tcataataat tagataaatc actcattatt atatctaagt ttccactagt ttctcctgct
     2761 tttatcatac ttataaagaa tttagaaaat atacctgtta tttgaaatga ttctgctatt
     2821 gagttacctt tttgaatatg atttgatact atactaagca agttctttac ttttttactt
     2881 gactgactct caatgatttc aaatattttt gtaatttcac atccagattc cagcaatatg
     2941 cctatctctt tgcaaattac tctaatttct ttatcactga ttctatttaa tactaaataa
     3001 aacttttcta aattataaaa tatattttta gataatctca tgtactataa taccttccaa
     3061 attatattaa ttacaattaa ataaacttaa tattttctaa tttaatgcat ttaattttat
     3121 ttcacttaaa tataatttat actcaaacat cttaattaaa atagattaaa taaagacaac
     3181 tgaccaaacg aagtttaact atacaattca gttaaatcat ttcatttact actaaacact
     3241 cttctaatgt cgttattttt tcatttatta gtcttttaca actatcttca aatgttatca
     3301 ttccattatt ttgtgctaca tctttaatag tactagaatc ctccatattt cttatacatg
     3361 tctttatatc atcattaatt tctaagattt cataaattgc tgttcttcca aagtatcctt
     3421 tatcattaca tttttcacac cctacagcta ccttagtatt tacatctcca ctaaaattat
     3481 cttttatcat aatctcatgg ctacaatgat tgcagacttt tctaaccaac ttttgagata
     3541 taacacctat aagagaggca tttaaaagat atggtggtat ttgcatttct agtaatctag
     3601 ctatggaaga tactgcatca ttagtatgta aagtacttat aactaaatga cctgttatag
     3661 ctgctcttac tgctatctta gctgtttctg catctcttat ttctccgacc attattatat
     3721 ctgggtcttg tcttaatatc gctctaagac ctacatcaaa gctcaaccct acttttgaat
     3781 ttacttgaat ctgacttatg ccatccatct tatactctac aggattttct atagtcataa
     3841 tatttttatt tattcctttt aaatcattta ataaggaata tactgttgta gtctttccac
     3901 tacctgtagg acctgtcact aataatattc ctgctctttt atttattata cgatttatag
     3961 attttatagc ttcctctgaa aaaccaagtt ctaatttgtc ttttaaaaaa gtatctctat
     4021 taagtatcct caaaactatt ttttcaccat agacagttgg tattgatgaa actctaatgt
     4081 caattaaatt tccacctaac tttatatcca ccctaccatc ctgaggaagc cttttttctg
     4141 ttatatccat tgatgccttt aattttataa ccgtagacaa tgatggatag acatcaacta
     4201 aaaactttga tacttccttt agttctccat ctaccctcat tctcactatt aaatgttctt
     4261 taaatggttc tatatgtata tcacttgcgt tttcttttat agctttttct aaaagatttt
     4321 caaataattt ttttgcataa tcctcatcaa gttcaaagct attataatat ttttcaatct
     4381 tttcacttat aagttctttt ttctccttct tcaatataat ttctttacct gtagctaatc
     4441 tcaagtcctg tagagcataa atatcctcat cttctatttc tacaactaaa ttatcatctt
     4501 gaaactcaac tggaaataca ttatattttt ttgcaagttt ttctggtatt aacattggca
     4561 aactgctttc ctctaatata atcttaatca ccctttatat actttaattt attaggtata
     4621 ttcatttttt tcatagagat attttcttca gtttttatgc ttattttcat tggataattg
     4681 gtttgtatgc tatctaaata tttactacct ttatctacat aattagatag tttctcctta
     4741 tctccaatgc actttatttc atatggttgt gctacaggaa ctccatttat atttatatgg
     4801 cttcctgcaa gtacaacttc tgaatattga ttaattcttt gaccatttat ttctatgtat
     4861 tttcctccgt ttacttttaa ttcatttata acttttacaa gaatcttatt atagtctata
     4921 ctattagcta tattccctgt tttttcatct atagcatcta tacttaatat tatcccacta
     4981 ccctttacat ctgaatatga tagactatct ctaagtgttt ctatttctga tatttcttca
     5041 gaattttttt gattttgttt cattgaatta atttctttat ctaacttgtc tttctctttg
     5101 gttaattctt ttatagattt tcttgtcatt tttatttctt ttcttattga cttgtctttg
     5161 cttgtataaa ctgtttcttc attgctataa tctctaaata tggttccaac aagtatacct
     5221 aaaatagtac ttgctagtat tataaacaca tattgttttt tcatactgta ttgctcctta
     5281 caaatttatt tttatatgat aattttcttt aattaaatat taaacattta catatgctta
     5341 ttatatcaaa gttatatatt aaaattcatc tttagtatat aatatcatac attgaactcc
     5401 tatcactaat ttaccaatat caagtatgca tttttagatg ttcttaaaat tatataaact
     5461 ttcaataaac attgttgtga tattataaaa aaacataaat taggaaagga aataataatt
     5521 atgaaaaaca aataatggct ttggagatta ttaatatcat tttttagcaa aaaaagtaaa
     5581 atggcatagc ctcaaagcta caccaattta ctaaaaatta tattattact aacgttaatt
     5641 ttaaacatat gcgtttataa gttttcctca tattttaaaa ttcttttaat taacttatct
     5701 cttaatttcc ctacttcatc aaatctcata ttaggactat agtaactttc tataatatca
     5761 tgtttagact tggctctctt aagatttagt actgcccaac aaattaaatc atctaaaact
     5821 ttttcatcaa atttaatatc ttcttcatat ttaagtagtt tttctctatt taaaaactga
     5881 tttaaatcaa ttgtatcttc ttctttaaaa gtcgtactta tagttatgcc tatatctaaa
     5941 tcagttaata gtacagtttc aattttctct ggaataagtg gataatgata aacttctaca
     6001 tttaaacctt tttcaatagc tttcttatat actttgttta ataatgtagt tttcccagtt
     6061 cctatttctc catctaaata atagactttc tttacatctt ttaaaatgtt ttctgtataa
     6121 tctaaacacc ctacaggagt tatagctgaa ccaaataaat gcttttctct ttttagattt
     6181 cctgtgcttt taactccatc aaataacctt tttataaatt cttctgtgac caaatttact
     6241 tttccataat ccatacaatc agaatatttt tcttctatgt ctctaaagat tggttctgct
     6301 gattttaaaa acttaaatgc tcttttaaat gaattactaa tatctttatc ataaactgat
     6361 atttcttgtt tattcttttc taattcttct gtattccagt aatcacctaa attaattatc
     6421 tcatccaaag ctccaggtag ctttgggtcc atagtatgtg gggcagttgc atccataagt
     6481 accacgccta atttctttat aactaaacca tccaacgaac ttgggtcaga aggacaatga
     6541 tataaatcaa tatcatatcc tctatgcaaa aactctttat aaattttttt cataagtgag
     6601 gattttccta ctccaggtcc accctttaaa caaaaaactc tttttacatt ttcaggaatt
     6661 atatagtcaa aaaaagaata tgagccttgt gatgtatttc cacccgggaa tgccttcttt
     6721 acctttcctt ccatattagc gcctcctcat aatttacaat atctcttaat taagatattc
     6781 atattataag aattttatta cagctttctg attttgataa atttaaaact taccatctaa
     6841 tactaataac aggttaattt tatctaatta ttatagattc tcatactgtt ccttattcta
     6901 tctataaata caatttaagt gtccatattg aaatatttgt attgtaatac agctggatat
     6961 tacttaaatc caattgtttc cattataatt ttatgttaaa ataatattac aaaatacatc
     7021 tgtttttctt cataaacggg tgaaattccc tatcggcggt aaaagcccgc gagccttatg
     7081 gcataatttg gtcatattcc aaagccgaca gtaaaatctg gatggtagaa gaaaatagta
     7141 tatgagtacc tttatgtaat tttacatgag taatctatac aaatccttca actaccgtat
     7201 ttattcatga aattagacac attcaaggta cctaatatac aggtgctttt tttgttgttt
     7261 attttacaat tatatcgtac ttataaaatc tattaagatt ggagtgttat catgaaacaa
     7321 aaatggatag tattgattat catctgtatt ggtgtattta tgtctactct tgatggaagt
     7381 atactaaata tcgcaaatcc tacaattgct gctgacttta aaattaacat gtcacaaatt
     7441 cagtgggttg taacagctta tatgcttgtt gtaacagcta ctatgttatt tttcggaaaa
     7501 cttggcgata aagtaggttc aaatcgtcta tacacattag gattttttat atttacaata
     7561 ggttcttttc tgtgtagtat gtcaaacaat ttatcaacat taatttcttc aagaatcttt
     7621 caggcagttg gagcaagtat tttaatggct actgggcttg gtatagtttc caatgcattt
     7681 ccagcaaatg aaaaaggaaa agctatagga atcactggag ctgtagttgg tataggaaat
     7741 atgagtggtc ctgtaattgg aggtataatt ttagaacatt ttggatggcc ttctattttt
     7801 attatcaata tacctattgg tattatagca gtctttcttg gcattaaatt tctacctaaa
     7861 cctgttttgg atgaacaaaa caagagcttt gatattccag gtcttctact tttcgcatct
     7921 tgcactacgc ttatattact tgcaatgaat gagaagggta atacaagatt gtatttaggt
     7981 ataacagcac ttataatctt cttattgttg gcattaagag aagtaaaatt tgaacaaagc
     8041 tttattgacc tacctctatt taaaaataga aactttactg taggcaatat cattggagta
     8101 gcttgttatt ttcctcaaat ggcagtatct tttctacttc cattttattt agaacaatta
     8161 aaaaatttat ctcctatgat ggctggttat gtaatgactg tacatccact tattatggta
     8221 cttatagcac ctattgcagg ttcactttct gataaacacg gtgcaaaaaa cattctaaca
     8281 gcatcttttt catttatgac catctcacta gttggaatgg cacttttaaa agcagattct
     8341 ccattgtatc ttttaattgt atgtttagtt atttttggac ttgggctagg agcatttagt
     8401 tcaccaaaca atagtagtat tttagcagat gttcctcctc aaaaacaggg atacggaggc
     8461 agttttcttg caactattcg taatttatca tttgctcttg gaacagcatt ttttagtagt
     8521 ttttttgcac aaagtcttac atacaatcaa aagtttaaaa gtcacacatc agcctatgtt
     8581 atagctagca atcagtctta ttggattgca gcatcagtat gttttatagg gcttatacta
     8641 actgttttct ttatgagaaa aacagataaa tctatttctt aaatgctatt tattgctgac
     8701 tacataagtc aataattttt tagttgaact aatttatact aattttccca taccttacta
     8761 aaatttatta gtatttaaca aaattactga tatttattgt ttataaacaa taaggatgcc
     8821 aaaaaaagtt cattaaaact attttaaggc atcctatatt ttgctttaat attaatataa
     8881 ttaaaactaa ctattattac atttattatt caaataaatc tcatgtcaat atttaacatt
     8941 ttaaatttaa tctactgtta tttcatatcc atgttgattg tattgattta caatctcttc
     9001 ccataattct tcaacaccag tcttttttag tgaagaaaca ggaattactt tttcactact
     9061 ttccatttgc aatttttttc ttattatact tatgtgtttt tgatattgtc ctctagatat
     9121 tttatctgcc ttagttgcaa taactacaca gttatatcca aagtgcttta tccattcata
     9181 cataagctta tcatctgctg taggctcatg tctaatatct acaagtagga atatagaaca
     9241 taactcttgt ctactttcta gatatcgttc cattatacct ccccactttt ctttttcaga
     9301 cttagctatc tttgcatatc catagcctgg aaggtctaca aaataaaatt cattatttat
     9361 tagataaaaa tttatagttc tagtttttcc tggtgtttga ctggttctag cgaagtttct
     9421 tctgttaagc aatgtattta ttattgatga ttttccaaca tttgacctac cagccaatgc
     9481 tatctctggg attccttctg ctggatactg tgatttattc acagcactca tagttatctc
     9541 tgaacttcta attttcatta ttttcaccac tctttaataa agcttgttct aatacttcat
     9601 ccatatgttc tacaagcaca aactccattt tttcttttac attttctggt atttcatcta
     9661 aatcagcttc acattcttta ggaattaata cttttgttat accagctcta tgagctgcta
     9721 ataacttttc tttaactcct cctactgcta agactctacc tcttaaagtt atctctcctg
     9781 tcattgctat atttccaggc acaggtcttt tagtaagtgc tgagattact gcaagagcca
     9841 tagttatacc agctgatggt ccatcttttg gaacagcacc ttctggaatg tgtatatgaa
     9901 tatcattcgt cttataaaat tcagggtcta tatcaaactt gtccactatt gaccttatat
     9961 aagagatacc tgtcttcgca gattccttca taacatctcc taattttcca gttaatacaa
    10021 tttctccttt acctttaagt acgtttactt ctacttcaag tgtaactcca ccaacttctg
    10081 tccatgcaag accatttact aatcctattt gagggttaac ttctgcaagt tgatacttaa
    10141 acatgtcttt tccaagatat gtctctaggt cagacttatt tataacaacc tcttcaagag
    10201 ttgggtcttc aacgtatttt ttggcaacct ttctacaaat cttaccaata gttctttcta
    10261 gtgttcttac accagcttct cttgtataat ggttgattat acttcttaaa gtttcatcat
    10321 ctatttttat aaagttttct tttaaagcat gttctttaat ttgttttgga agtaggtatt
    10381 tttttgctat atttaatttt tcttcttcta tatatccaga tacttctatt acttccattc
    10441 tatctaataa tggtcttggt atattactta gactatttgc tgttgtaaca aatagtattt
    10501 ttgacaaatc aaaaggtatt tctaagtaat ggtccacaaa atccttattt tgctctgggt
    10561 ctaatacttc tagcattgct gaagctgggt cacctttata atctgctgcc atcttatcta
    10621 tctcatcaaa taagaataca ggattttttg tttgggcttc ttttacccca ttaattattc
    10681 ttcctggaat tgaacctaca taagttcttc tatgacctct tatttctgct tcatctctaa
    10741 ctccacccaa agatattctt acaaattttc tatttaatgc tctggcgatt gattttacta
    10801 tagatgtctt tcctgttcct ggaggaccaa ctagacatat tataggccct ttaagtgact
    10861 tagcaagagt tcttatagct agatactcta gtattctctc cttaactttt tctaatccat
    10921 aatggtcttc atctaatata tcttttgcct tagttatatc taatttatct tttgtctctt
    10981 tattccatgg caatgagaat atagtatcta aatagtttct acttacagat acatctggag
    11041 acatagaaga tatttttgaa aatttatcta tctctttttc tattttctct ttagttgtct
    11101 taggtgcttt tattttttta agcttttctc tatactcatc tgcttcagag tttatatctt
    11161 cttcttcacc taattctttc tgtatagctt ttaattgttc tctaagataa tattcttttt
    11221 gaaccttatt catctgtttt tttactctta aagttatttt cttttctatt tttaatatat
    11281 ctatttcctc taataatata gaataaataa gttcaagtct ttttctaata tcaaattctt
    11341 ctaatatttc ttgtttttgg cttgatttta gatatatgtt tgcagctatc gtatctataa
    11401 atctatctac atcttctata tcagctaaag atattaatat ttctggtgat accctatttc
    11461 caatatttat atactcttca aaggcatcaa atacatttct tacaaatgct tcaatttcaa
    11521 cttctgtttc actatccaaa ttatcactat caaatactat ttcttctatt actgctctga
    11581 aatacccatc ttcttgttct attttcttta ctctacccct tgatacacct tcaaccaaaa
    11641 cacgaactgt atctcctggt aattttatca tttgcttaac tttacagatt gttcccacat
    11701 gataaaaatc ctcttctcct ggctcatcta cctctgcttc tttctgagaa gttaaaaata
    11761 taagttcttc atccatcata gcttggtcta aagctttaag agatatctct ctacctatat
    11821 caaaatttaa tatcatatat ggaaatatag ctaatcctct tagaggaatt aatggtaatt
    11881 catgatctat tttagtataa ttttgttcca tcaacattac tccttttcta gtctaattgc
    11941 tttgctttta atattatata tttgaacaag ttaattttca acattaatga taaattctta
    12001 gaatactatc atactatatt atctttttat tatgatataa tattaatatt agtatactta
    12061 gatgttattt atcttttgga ggtttctatt atgttgaaat tgtttttaac ctttttaaaa
    12121 attggtgcgt ttacatttgg tggtggatac gctatggttc ctcttataca agatgaactt
    12181 gtaaacaaac aaaaactttt agagcaggaa gaatttattg attatttatc catagctcaa
    12241 agttatcctg gagtactggc agttaatata tctgtattat taggatataa actttatggt
    12301 ataccaggag ttcttatatg taccttaggc tctgctttac catcattttg tatagtctta
    12361 gtcttatcgt atctatactt taaaaattca tcatctaaaa ttttagatgg attttttaaa
    12421 ggagttgcgc ctgttgtaat agctcttata ttgtattctt ttacgagcat gttcaagaaa
    12481 ctaccaaagt ctaaaactaa catactacta ttaattatag cagttttatc tgttggtgta
    12541 ttcaatataa gccctattta tttaataatt ggaggaggtg tatatagtct atgtcagaaa
    12601 taatactact cttcttaata ttccttaaaa taggggcatt tagttttggt ggcggttatg
    12661 ccatgctacc atttatccag caagaattta tatctaaata tcactttata actaatcaag
    12721 agtttttgga tttacttgct atatcacaat ctacaccagg tcctgtagca ataaatagtg
    12781 ctacatttgt aggatttaag actgctggct tctttggttc tctagctgca actattggtg
    12841 ttacaatttt tgcattaata ggtctaacta ttatatcaaa gttattttct aaatttaaaa
    12901 ataataaaaa ggtagaaaac ttattgacag ttcttagacc aattactcta ggattcatat
    12961 tatcagcagc tttttcatct gccaaagaag ttaattggga tttatatgga ataattgcat
    13021 ttatagcatc attttatctt ttatataaag gaaaaattgg ttccattgca attgtattcg
    13081 tctttggtat tctaggtata ttactcacat ttttataata atgataccta ttttatattt
    13141 agcatattaa cataaaaaaa ggagctttta aagctccttt tcatcatctt atgcactttc
    13201 ctcttgatcc ttaagtacta tgataggatt tactgagtct tcgttcacag atttctctgt
    13261 tactataacc ttctttatat tatctctaga tggtacttca aacatagttt ccatcattac
    13321 gctttcaact atacttctaa gtcctctagc tcctgtattt ctttctatgg cctttttagc
    13381 gattgcacga agagcacctt cttcaaattc tagctcaaca tcatctaact ccaataactt
    13441 tttatactgt tttactagag catttttagg ttcctgtaat atctgcatta atgcatcttc
    13501 atccaacaac tctaaagtag ctaaaactgg tattcttcct ataaactctg gtattatacc
    13561 atattttaat aaatcctctg gaagtacttt ttcatacagc ttccctaaat ctaattcttt
    13621 tttactttct attttagctc caaaacctag tgttttatct ccaccacgtt tttgaattat
    13681 cttctctaat ccatcaaaag ctccacctaa tataaataat acattagtag tatctatctt
    13741 taaaaattct tgatgaggat gttttcttcc tccttgtggt ggaacatttg caacagtacc
    13801 ttctaatatt tttagaagtg cttgttgaac accttcacca ctaacatctc tagttataga
    13861 tgggttttca gattttcttg caatcttatc tatctcatct atatatatta tacctctttc
    13921 agctttttct atatcaaaat cagcagcttg aataagtttt aaaagtatat tttcaacgtc
    13981 ttctccaaca tatccagctt cagtcaatga tgtagcatct gccattgcaa aaggtacatt
    14041 tagagttctt gctagagttt gagcaagtaa tgtctttcca gaacctgtag gtcctaataa
    14101 aagtatatta ctcttttgaa tttctatatc cttactacta gattttttac tgtatattct
    14161 cttataatga ttataaaccg caactgataa agctttttta gctttttctt gacctattac
    14221 ataatcattt agaatctcca tcatttcctt tggttttggt aaagatgtag tatcttcatc
    14281 tatagtatct tctatttctt cctgaataat ttcgtcacaa agttcaacac actcgtcaca
    14341 aatatataca tttggacctg caattaatct tcttacttga tcttgatttt taccgcagaa
    14401 tgaacacttt aattgtcgtt tttcttcata tttagacata ttaacacctc tctctatcta
    14461 cggtctcttt gtaaatactt catctattaa accatattct ttggcttcta gtgcactcat
    14521 gaagttatca cgttcagtat ccattttaat tttttctaat ggctgaccag ttctttcaga
    14581 taatatctca tttaaagttt cttttatttt taaaattctc tttgcatgaa tttctatatc
    14641 tgttgcttga ccttgagctc cacctaatgg ttgatgtatc attatttcac tatttggtag
    14701 tgctagtctt tttccttttg ctcctgctgc caacaagaat gctcccatag aagcagccat
    14761 acctatacat atagtactta catctggttt gatgtattgc atagtatcat atatagccat
    14821 tccagaagtt atacttcctc ctggagaatt tatatataaa tgtatatctt tgtctgggtc
    14881 ttcagcttct aaaaataata gctgtgctac tataagccct gctgtggcat cgttaacttg
    14941 atctcctaag aagattattc tgtcttttaa tagtctagaa aaaatatcat aagatctttc
    15001 tcctcttcct gtttgttcaa caactacagg tactaatgcc atggtttcat acccccttta
    15061 ttctatataa tttacagtag ctttttttaa gctactgtaa attattatac gcatttaact
    15121 aaattatatt tgcacatcat tatatactaa acatttcata acgtcaacta tattagttag
    15181 cgtttatttt attaagctat tgttgcatta tcaactaata aatctattgt ttttcttatt
    15241 tttatttgac cttttatatc ttctttgtca gcatctctta aagaagcttc tattttttct
    15301 acttccatat tgtaagcaga agccattttt tctaactcag ccttaaattc ttcttctgtt
    15361 gcttcaattc cttcagcttt agttattgct tctaatacta aagaagactt aactaatttt
    15421 ttagcatctt cttttctctc ttctcttaat tcttccatag ttctaccagt catctctagt
    15481 aattgttgta atccaaatcc ttgatattgt aattggtagt taagttcatt taacatatta
    15541 tctatttgat gttgtatcat aacttctggt atttctattt ctgtattttc agcaactttt
    15601 tctactactg aatttctagt ttctgcatct gctctatttt tagcttcttc ttctaactta
    15661 gctcttacat ctgcttttaa ttcatctaaa gaatcaaatt cactagtatc tttagcaaat
    15721 tcatcatcaa gtgcagataa ttcttttact tttacatcat ttatttttac attaaataca
    15781 actggttttc cagctaaatc ttgtgagtga tattcttctg ggaaagtaac attaacttct
    15841 acttcttcac cagctttttt acctactaat tgttcttcaa atcctggtat aaatgtgttt
    15901 gaacctataa ctaagttata attttcacct ttaccaccat caaaagctac accattttca
    15961 aaaccttcga agtctattat agctgtatct ccatcttcta aagctttgtc ttctacactt
    16021 actaatctgg catttttttc aaccatttct tgtagtttag catcaacatt ttcgtcactt
    16081 acagtattct caaccttagc tatttctata cccttgtagt ttcctaattc aaactctggt
    16141 ttaacttcta cattaagaac cataaccaac ccattgtctt tacttatttc ttctatatct
    16201 aaatctggat tgtctattgg gtctatgtct aattccttga tagcttctgg ataaacttct
    16261 gggaataata tttctatagc atcattgtag aatatacctt ttccgtattg agtctctata
    16321 acaattcttg gagctttacc ttttctaaat ccaggtatat tgtatttatt tctgctttta
    16381 ttataagcct tatttactgc tgcttcaaat ttattgttat ctactgttat ttttaaagta
    16441 actttattac cctctttttt aattagttct gctttcatcc ttctaatcct cctagtgata
    16501 tttatgtaat acccgataac ggtattataa ttcattctat ttataattca accacttaat
    16561 tatatcacat aagttttttt atttacaagt gttccattaa tatcgttgta cttatttgat
    16621 aaaatttaat tttttgagcg ttttttgcca tttttttaga taaatacacc tttatatgcg
    16681 tatttcttca atttttatgt catcttcttc tatatccata atgacaaaac ttttatacga
    16741 tactcctctt ggaagtgaag tgcttccagg atttatgtaa agtacaccat ctttaatttc
    16801 tctaaatggg gtatgggtat ggccaaatat aactatatca gcatcaactt cagttgcctt
    16861 tttttctaac atattagttc catattttac accatactta tctccatggc ataagaatat
    16921 ggttttattt gccacttcaa agacaacttc ttcctcaaca gcatcaaagt cacaattacc
    16981 tttaactgca ataataccta cattagtcat gctatgaata tatcttgaat cagtaaagtt
    17041 atctcctgca tgaattatta ggtcacattc tttcaaatat ggtattgttt tttccatatt
    17101 tttaatcatc atatgtgtgt cgcttactat gcctattttc attacttcac tacccttttt
    17161 ataaactctt gtttcataag ttttagtgcg tttgctctat gactgattga attttttata
    17221 acactaggta tttcaccaaa agtcttatca tatcctttta ctataaataa gctatcatat
    17281 ccaaaaccat ttttaccttt ttcttcaaat cctatcattc cttcacaaat tcctcttact
    17341 acaaattctt ttccatcagg aaaaactact gctattgcag agacgaatct tgcatttctt
    17401 ttgctcattg gtacattttg catagctttt aacagttttt ttctattttc ttcatctgtt
    17461 gcatgttctc ctgcatatct agctgaataa acaccaggtt ttttgcctaa agcatctact
    17521 tctaagccag aatcatctga tattgctata agtttagtct tttttgctat tgttctagct
    17581 tttatcaacg cattatgctc aaatgttttt ccatcttcta ctatctctat acctgctaaa
    17641 tcaacatctt ttaaagagta cactttatat tcaaaatctt ttaagatttc acctatttct
    17701 tctagcttat gagcattatt agtagcaatc actacttcat taccataatc catacccaat
    17761 atctcatctg atatttcacc taaagcttct ctttgaagcc ttattaattc tttattccct
    17821 ttttcaccta actctaacaa taaatttaaa tctgctctag aaaaaggttt ctcttctcct
    17881 gtcccctgta cttctacaaa ctcacactta tcagtcatta ttatattcat atctacctgt
    17941 gctttagaat cttcttcata acataaatct agcatgtgta ttccatttac aataccaaca
    18001 ctaattgcag acacaaaatt tgttattggc atctctttta ttgtctttgc tttatataat
    18061 ttatatattg cttctgcaac tgctacaaat gcccctgtta tagatgcagt tctagttcca
    18121 ccatctgcct gaatcacatc acaatctacc caaatagttc tttctcctaa tttattcaag
    18181 tctacaacag accttattgt tcttcctatt aacctctgta tttcttgtgt tcttccatca
    18241 atttttcccc tagatgcatc tcttatcttt ctttgttgtg ttgctcttgg tagcatagaa
    18301 tattcagcat ttatccatcc agtaccacta tttcttaaaa aaggaggaac tttttcttcc
    18361 actgatgcag tgcatataac ctttgtctct cccatttcta taagaacaga accttctgca
    18421 tactttgtat aatctctagt tatctttaca ggtcttatct gttcattact tctatcataa
    18481 cttctggtca ttttttatat cctttctcat attttttatt ttttataaat ttatttttat
    18541 aatttttaat tttgtcaaat attaattatt gtcatttttc gtaatcttat tagcttactg
    18601 aactattcta tcattaatta tagtagtttt ctactaaatt aagcgacttt ttaactctct
    18661 aatacagctt ttacttcatc tttcgatgat aactttaata attctaaaaa tctctcttca
    18721 tctataactt taattcctaa atcatttgct ttagtaagtt tagaaccagc ttcttcacct
    18781 gccaaaacaa atgttgtcga tttactaaca ctagatgtag cttttccacc tcttttttct
    18841 atcatatctt ttgcatcatt tctttttaat gtaggaagtg taccagtcaa tacgattttc
    18901 ataccttcaa atattttttc tactttttca cctgtattat ccaatgattt tgtatttact
    18961 cctacatttt ttaacttgtt tataacgttc atatttttgt cttccttaaa aaactcgaca
    19021 acgctgtttg ccattatact tccaaattcc tctaattctt ctagttgctc actagttgca
    19081 gaaattactt catctaaatc cttaaagtta tatgcaagta tcttagctgc tttaactcct
    19141 ataaatttaa tacctaatcc atttataagt ctccataaat cattttcttt tgatttttca
    19201 attgaattta ttaaattgtc agcagatttt tcgcccattc tctctaggtc gactacatct
    19261 tcctttttta tataatataa atcagataca tcttgaataa ttttttcatt taatagcagt
    19321 gtaattatag attctcccaa tccatctata ttcattgcat cccttgacac aaagtgtata
    19381 attccccttc ttatttgggc tgggcaagac atattgatac attttacagc agcttctcct
    19441 tctaatctta ttgttggctc tgaacaaact ggacactttt ctggcatttt aaaatctatt
    19501 tcatctccag tacgttcttc ttttataact tctaaaactt gaggtattat gtcccctgct
    19561 ttttgaacta atactgtatc atttattttt atatcttttt ctcttatata atcttcattg
    19621 tgaagtgtag ctctacttac agtggtacct gcaagtctga ctggttcaag aattgctgaa
    19681 ggtgttattg taccagttct cccaacttct actattatat ctactatttt agttttcttc
    19741 ttttctgctg ggaatttata tgcaattgcc catctaggac ttttggctgt atatcccatt
    19801 tcgtctcttt gttttatatt gtttactttt attaccatac catctatttc gtagtctaaa
    19861 ttacccctgt tatctgtcca gtattcaata tgctcaataa cttcatctat acttttacaa
    19921 actttataac tctgacttac tgaaaatcct agtttttcta agtactctag tgattcactg
    19981 tgactttcta aatcgtattc ttctatattt tccatattaa atacaaatat atctaatggt
    20041 ctctttgctg ttagttttgg gtctaattgt ctcaatgaac cagctgcaag attccttgga
    20101 ttagcaaata atggctgttc ttgttcttct tgttgttcat ttacttttct aaaattatct
    20161 tttgatatat atacttctcc tctaactatt agttcttttt tatcatttat cttaagaggt
    20221 atgcttttta ctgtcattaa gttttgagaa atatcttctc ctacaactcc atctcctcta
    20281 gttgctcctt tttcaaactc tccattgtta taagttagac ctacagaaag accatctatt
    20341 ttaaattcaa caacatattc aacattttct cccacatatt cttgaactct cttatcaaaa
    20401 tctctcaaat cttgctctga aaaagcattt gataaactca acataggagt tttatgtaca
    20461 atttgattga acttatctaa tggcttacct cctaccctat ttgttggaga atcaattctc
    20521 tttagttctg gatgttcttc ttctaaagaa attaactcct tcattaactt atcgtattca
    20581 taatctgata tttctgggct atcttgatta taataccttt cattatggta gtttattgtc
    20641 tctataagtt tattaatttc atcttttacg ctcacttttt gcacctcttc atatttttaa
    20701 aattaaatta ttttgaaatt tccctataat taattatctc ctatttatat attttttagt
    20761 atatcacttt ttgggcttta aatttattac aaaaaaaact cttattagta tctttaatac
    20821 gactaataag agtttttatt tttatcaaaa ttttttattt ttaatagcct cttgatatgt
    20881 ctacaacatt atctacaggt ttattctcca ttatatagtg tttcaaatta ttataaacca
    20941 tattaaatgt tctctctttg tttttatctg acacccaaga attatgagga gttactatga
    21001 cattttcgca ctcccacaac ttattgtcct tgtttaaagg ttcactttca aatacgtcta
    21061 atgctacccc tctaaatttc tctacacatt cttctaaatc tttctgatta actgtatttc
    21121 ctctacctac atttataaat acagaaccat ctttcataag tttaaacata tctttgttta
    21181 ctattccatc tgtacttttt gtagaaggca ttgctacaac tactatatca cattcttcaa
    21241 atacaatatt catatcctca gtagcaaaac acttatcaaa gtatttttta ctacttccat
    21301 cagtattgac accccatatt tctacaccaa aagcttttaa tctttttgct gcttctgaag
    21361 atatagtacc tgttcctaaa aatccaactc tttgtccata caattctgat acagaaaaat
    21421 ccatcttcca ttctttattt tgttgttgtt taaaaaattt tgcactattt ttatatattt
    21481 ctaaaatata caatacaata ctttcaccaa taggtacact atatccgcct ctattattag
    21541 ctatcttaat atttctcttc aatacctctt cttttggaag ttggtctata cctacactag
    21601 ttgtctgtat atattgtaaa tttttcatct tagaaatatc taatctctca aatggattgt
    21661 atgtaactag tacatctgct gtatttacat cctcattatt ttcaatacta tcttcagagt
    21721 aatgtatgac atcatagcct aattctctta ttttatcaaa tttttctttt ccataattta
    21781 ttgtaaataa aactttcatc tcaaatctcc tccatctacc cacatttaaa attttctatt
    21841 ctaaaatata atatgatact caatgtacat tagttaaaat tactataaga tatcttaaat
    21901 ttacttatgt tcaattgttt ttcctaaaga catagcatta cgttcttgaa ctaaattttc
    21961 tattaaatta ttcaatgtcc aatcaataga tgtctctgtt accatagctg taaaaggtct
    22021 atttattctc acttttttta gcccttctaa aaatgcattt acttgtgtac ttggagtgtt
    22081 attaaatatt acagacttac tcttaatccc attttcacat tcatttgata tatttccatc
    22141 taaaatgtct cttattatat tctctccatt tttatcactt aagatgatat ggtctcttat
    22201 tccagcaaag ttacttacat ttttaattaa tgataattct tttttattaa agtttacaag
    22261 cataatacac ttcctattgt gtattttatc taggttatta ttatttattt tttcaaatga
    22321 cattttatcc atcctttcat ttatttatat actttaattt accccataca tttaaaataa
    22381 tcaattttta tagttaattt ttatattaat acccaaatta tatttaaata gttaggctat
    22441 ccaaaactaa tatataaaat tagaataatt agattaatat ctacaaatca taacctaaag
    22501 gagagatacc atgaatacat tactaaacaa taaatttagc attaaaaact tttcaaatgc
    22561 ttgggttata gtacttggtt gtatgttaat acaagctgtt ccatttagta ttgtagcaag
    22621 tatacagcct caatttataa gttatgttgt agaaggagaa ggctttagtt tagcttcttt
    22681 ttctctaata ttcactattg gtactattgc ctcagcaatt gcatcacctt ttataggtgt
    22741 attattttct aaagtcaata ttaagatact atatttatta ggttgtataa tttctagtat
    22801 tgcttttgct actttttcta tatgtactga actatggcaa ttttacactg tatcatgtat
    22861 tgttcaaatt ggcgctacaa ttatatctgc tataggaata cctgttttaa taagtgcttg
    22921 gtttgatgaa gaaagcaaag gaaaagcttt gggctttgct tttgctggtg gttctattgg
    22981 tagtatattt ctacaaccac ttgcagttcg cttattagca actcaaggat acagtcgttc
    23041 ttatctaata tttggaatta tagcattaat agttggtata cctgtaatat tattcatact
    23101 caggatgcct aaagatatta atgaaaaggt aaaatctaaa ggtaaaaaag agaaagcatc
    23161 tgatacaatt tctccttcag tatggggtta tacattaaaa gaagctaaaa aaattaaata
    23221 tttttggatt atgactattg gatttatatt tgttggtata tatgtttcat cattatctgt
    23281 acaatatcca acttatctta gaaattatct aaatcctatt ctaataggaa cagtaggctc
    23341 cacatttgcc ttatttgctc tcattggtaa tttatttgga ggattattgt ttgataaatt
    23401 cggtcaaaca aagccactta ttatatcttt tgtattggtt cttatagcaa ctttatcatt
    23461 actattggta gataaatctt ctacttttgc atttgtattt gccattttga aaggtctttc
    23521 tgtatttgct tatatgattg gtcctgctta tttaactggc tctttctttg gtcaaaagga
    23581 gtttggtagc atatatggaa tcgtacaatt aatttttgca gtgggatttg cattaggttc
    23641 aacattattt ggattgttag ttgataattt tggatacaga ttttcttggt ctattataat
    23701 tttatcagtt ataattgcct atactttact tattataact tctataggaa tgaaaaaatt
    23761 gaataaagaa agattagata aactaatgca ataattgatt tttagatata taaatattat
    23821 aataaaaaag atggagcttt taatcataaa taaaactcca tcttttttat attacaatat
    23881 agctaatttt ctcttatttt taactttact atctatatcc tcatccttaa ctatagtaaa
    23941 tgccttagtt ggacaaacat ttacacatgc aggcccttct ggcgattcta tacaaaaatc
    24001 acacttattt gctgtcattc tttctctaaa gcaaagcttt ccttcatcta catcttttaa
    24061 tgtaactatg tctattgcac ctataggaca tgctaacata caagtcttac atcctataca
    24121 attttcttca tttattttta ttgtgttacc aaccctaact ataccaccat ttggacatgc
    24181 ctttgcacaa ggagcatctt cacaatgtct acactgtatt ggagcactta cttcttttgt
    24241 ctttatgaca gaaagttttg gattaaaatt tatttcatct ggattttggt aaaaaatatc
    24301 ttcatctgaa tgtgcaacta cacatcctat catacaagtt ctacatccta tgcatttatc
    24361 agggtctgct ataacaaaat agttcatcac ttagccactt cctttcctat acaatatgta
    24421 gtgtgtaata gatgatgaga tttttctcct aatggttcta ccagatattc atcatacagt
    24481 tttttaatat caggattctc atgagatttt ctaattgcta aatgttcatc atgcacgtat
    24541 gttgcttttg ttctattttc ataagccaat tccttatatt cttctagtaa aatttttggt
    24601 tgacctccac cacttacaca cccaacagga caagtcataa cttcaataaa atctacatct
    24661 aattttcctt tttccaagtc ttcaagaact ggtattacat tttttaatcc acttactact
    24721 ccaactctta agtctaagtc tcctatttta atagttgact ttctaaaacc attttctcct
    24781 cttacttgtt ttacttctac atcaggaata ctttctccag ttataagctc atatcctgtt
    24841 cttatagcag cttccataac cccaccagta acaccaaaga tagttccagc tccactataa
    24901 ctacctaaag gactatcaaa tttttcttct cttaaatttt taaaatcaat tcccatgtct
    24961 tttattaaat aagctagttc tctagtagtt aatactacat ctacatctct atatccacta
    25021 gaatccattt cttctctatc acattcatat tttttacatg tacatggcat tatagaaaca
    25081 ctaaatatct ttgatgcatc tacattattt atctctgcac cataagtttt aaatattgca
    25141 ccacccatct gctgaggtga tttacaagaa gataaatgat tagttaattt aggataattt
    25201 ttttccatga attttaccca agctggacaa caagatgtaa acattggaag agttccacct
    25261 tctgtcactc ttttaattaa ttcactacct tcttccatta tagttaaatc tgctgcaaaa
    25321 ttagtatcgt agactctatc aaaatttaaa gctctaagtg ctgctgccat tttcccagca
    25381 cctaagtatc ctaaataata tccaaaatct tctcctaaag caactctaac tgctggtgca
    25441 cattggacca tagtgaatac atttgggtca cttaaagctt tcttcacctt atctatatca
    25501 catacattgt atgctgcaaa tataggttca tttatagact ctggtatctc tctttcttgt
    25561 tttttctctt gtaaaaactc aaatccttca tctataactg aggcataaga tttacaagtt
    25621 tgaacacatt gaccgcacat aacacatcta tctaaattta tctcttgtgg ctttccctct
    25681 tcaccttcaa tagcatttac tggacagacc tttgaacatt ctctacatcc agtacataaa
    25741 tccttatcta tatttattat cataaacgtc acctacccct gtatccttta tttatttgtt
    25801 aacaataaac taagtgcagc ttgtctattc ttagcttttt tgtcatctat tggagtaaca
    25861 agttttaatg cttcctgagg acatgcactt atacaagcta ttttttcatt gtctttacat
    25921 aagtcacatt tataagctat cttcttattt tcatctaaaa ttacttgttc tacttcttta
    25981 ccatcttcat attgtggaag taagtctata gctccaaaag gacatgctag cagacaagtt
    26041 ttacatccta tacacttttc ttcatttaca gacataatat tgttttcttt aactatagct
    26101 ttttgtggac atgagtttaa acaaggagca tcttcacaat gtctacactg tattggcata
    26161 cagaaattgt caccttttac taagtaaagt ctaggtataa caggtatatc aactgttcca
    26221 actgtataac ccacattatt atgctgatta tgcattgtaa aacaagctac ttcacaagct
    26281 ctacaacctg tacatttgtt tgggtctgct attacaaaag agcctatttt atttttcata
    26341 ttatcccacc tttcaatttg tatatttcca accctctagt tattttcatc atatgtctac
    26401 ttactttcat catatatatt tatatatttc actattttct cagtacttat atttcgttta
    26461 cattttttgc acaaattgta tgtgttatta tacaccttat cagtatcatc aattaatact
    26521 ccacagattt cacactttga atatctttgc tcttcttttt tagtacacat atattcattc
    26581 tcactttttg ataactctct gtggatttgc ataaatattc atccgtcttc ctcttacaaa
    26641 tccaataagc gttatattta attttttaga aagctctatt gataaattag tcggtgctga
    26701 ttttgatact acaataggaa tttgagtcat agctgctttt aatatcattt ccaatgatat
    26761 tcttccactt acagccacaa ttttatcttt taatgttatg tcatttaaaa cacaaaatcc
    26821 aatagcctta tccattgcat tatgccttgc tacatcttca caagtgacta tttttttatc
    26881 aaaattaaat acagatacgc tatgtacccc accagtattt ttaaataatt ctgatgaatt
    26941 taaattttta tccattatac tgtaaatcgt atcataattt atttttatat tgctctttac
    27001 aggagcacat gttatatagt ctagtgaatt taaaaatata atttgctcat cttgattttt
    27061 ttgtttctct tgaatagtta cttttgcaaa acgttcactc tcatttatct caaattttaa
    27121 taagcttttt ttgttttcta tgtatccttt tgttttcaag aatcctacta taagttcctc
    27181 taaattatat ggggtacata aaaatgtatt tacatattta tcatttaaaa taaaactaag
    27241 tggatattct gcaattattt cttcattatc tattgatact tcatgttcat ttattttgct
    27301 tacttctaca ttaaaagaat ttaaatattt gtagtccact agactataac tattttcaaa
    27361 aactttatcc ttactatttt cttgtaattt agtttttgag tatatattgt tcataaaata
    27421 ttagcctttc tgccttttat aaatcttaaa tctaataaat cattagatat ttgtagctgt
    27481 cattttgtct ttcaaacttt gatactcttc tcttacacat ttttctgcta attcttggtc
    27541 ttcaagtttt tcaagtttaa cagcacaata tttatactct ggtgttttag atatagggtc
    27601 taaatttgct attgtaagtt cattgcaagc tccaacccac cactgataag tcatatatgt
    27661 tgcaccttcc ttaactctat cagtaactgt tgctcttgtt attacacttc ctcttcttga
    27721 actaattcta actaattcat catcttcaat tcctaatttt tcagcatcat tagaatttat
    27781 ttgaacacgt cctggttcat cttctaatga actaagtgtt ctacaattac cagtcattgt
    27841 tcttactgaa tagtgtccaa cttctcttac agtacataaa ctaaatgggt attcatcatc
    27901 ttcaacttcc atagggggtc tccattcagc agcgaacaaa ttacctttac cattaggagt
    27961 agaaaacttt tgaccttcat acagatacat agttccttta tcttccatac tttcactctt
    28021 acaaggccat tgaacgcatc cttgagtttc tatcttttca taagtagcac ctaagaaact
    28081 tggacataat tgtctaagtt cgtcccatat ttccttagta tttttataat tcataggata
    28141 tcccattgct gtagaaattt cgcttataat ttgccagtct ggttttacat ctccttgtgg
    28201 ctctatagct tttctcatca attggaatcc tctatctgca catgtatata ctccctcatg
    28261 ttctccccaa gaagtagctg gaagtataac atcagcatga agtgctgtct tattcataaa
    28321 tatatcttga acaatgacaa attccaattc atctaatgct tctctaactt ctgatgcatc
    28381 tgggtcactt tgcactgggt cttctccaaa tatataataa gctttaagct ttttctcttt
    28441 tagtactaaa tttggaactt gagttaaact ataaccatta ttaggagata attttacccc
    28501 ccaagctttt tcaaactttt ctcttatctt atcatcagtt acattttgat agccaggata
    28561 tacattagga agagctccca tatcacaagc tccttgaaca ttattttgtc cacgtacagg
    28621 tcctattcca acgctttctc taccaaaatt acctgtaagt agagcaattg atgcaagtcc
    28681 cttaactaca tcaacagctt gaccaaattg acagacaccc attccataaa gtatcatagc
    28741 ttttttacct tttgcatatt ctctcatagc ttttcttata agctcttctg ggattcctgt
    28801 aatcttttca gcatatttag ctgtatatgg tttaacaatt tctttatact catcaaatcc
    28861 ttgtgtatga ttttgaacaa attctttatt gtaaagacct tcttctatta atacatttcc
    28921 aaaagcattt actaaaacca tgtttgttcc accttttata ggtagatgta tatcagcaat
    28981 tcttgcagat tcagttactc tagggtcagt aacaattaat ttagcaccat tttttttagc
    29041 ttttactatt ctatttgcca ctattggatg tgaatctgct ccattatatc caaatataaa
    29101 taaaacatct gcattttcta tctctggtat agaattagac attgcaccac tacctaatga
    29161 gtaagccaga ccggctacag atggtgcatg tcagacacga gcacaatggt caacattatt
    29221 agttcctatt gtagctctca taaatttttg cattatatag tttgcttcat taccaggtcc
    29281 tcttgcagaa cctgttccca taatagaatc aggtccatat ttttctttaa tttcatttag
    29341 tcttgaagct gtatagttta tagcctcatc ccattcaacc tcttctagta caccttgttt
    29401 tcttatcatt ggctttttta gcctaggtgt tagaatttta gggtcattta gaaaatccca
    29461 tccataacga ccttttaaac acaaactttc ttgatttgtt ctaccattag ccggttcagc
    29521 tcttaatatc ttattatctt ttactactag atataattgg catccagctc cacagtatgg
    29581 acaaactgtt aaaatttttt tctccataca attcccccct tagatttatc ccctgataag
    29641 taaaatttat cttaaagata atgagtgttt ttataatatg caaaatcata aatattttgc
    29701 aatactatct attaaattca acaattttct cacaattcct cttaaatttc aaaaaaaata
    29761 tgtcataata atctttatat ttcaaccatt taattaaaat attctagttc ttatgacata
    29821 ttttttaata tactcaatat atattcttta aacttattta gttttcactt aaaacaatat
    29881 ttctgccaat tctgtttttc attctatagt gagtaattac agtaaatatt ccacttccaa
    29941 tcagaatcaa atagtacata aatccaccat ataaaaatac tgcatatccc atcaaaggtt
    30001 gagggaatac tgatttgaat atagtgaaaa aggctaattc attagctcct acattcccag
    30061 gtgttggtat tggagatatt gccatatata aaaatgcttg taatgtcagt atataaatat
    30121 agctataacc ttgtaaatta aatgctttat atatccaaaa tgaaacacta aagtaagcac
    30181 ttaattgaat aaaagttact attatagttg aaattaatac ctttttattc tttgcaaaaa
    30241 aatttattgc tttactataa tcatctataa aactttctat ggattcaatc ttattatcta
    30301 aaaattttaa aaacttaatt tttgataaat gctttatagt aattctcata aaatatttta
    30361 ttttttcagg atttaaaata actaatatta tcataattaa gacaaatgaa ttaatggcta
    30421 tccctagaaa cactagaggc ataatcattt tcatttgttt ttctaacata gcaaaattca
    30481 ttaaaacaag tattgtacaa taaaaagtaa ctactatttg gaagataata gacttattgg
    30541 taacaactgc acttgcctta ctaagtggca ttttacattt tttaagtaca tatatctgta
    30601 ctggttggct tcctgaagca aaaggagtta ttaaattata ataaaatccc ataatggcta
    30661 gcttaaaacc aataaatcta atattaactt tgtgagtgct ttctataatt atcttcatta
    30721 ctactccttc aagcattatg tgacacatta ttgccaaagc acctatgaat aaaaacttct
    30781 tatctaccat aaccataaca tttgatagca ttttaatatc caatgtttta aacactaggt
    30841 aaattgttat acctattaac aaaaataaaa atgtatactg tagtaaactc tttctaatat
    30901 tctttagtct atccattaga aataaccatc ctttgctcaa gatttatact aaatactata
    30961 ttattagtat acactatgaa tcttaatatt ttataacaaa ataccttaca attttgtcat
    31021 ttacaaaaaa tattaatcta taaattaaat tttaatatat agattaatat tttacaaaca
    31081 ttttgtagat atatataata ttttcactaa acaacactta tacttattac acccatttat
    31141 accctatccc ccatacagtt tctattgtat aaatatcata ttgttttaat ttgcttcgta
    31201 tattttttat atgctctgtg atagtactta tatcactttc tccatcaaaa ccatataccg
    31261 actcatatat tctttctttt gaaaatactt gtcccttatt ttttgctaga aattcacata
    31321 ttagatattc acttttagta aaattaattt ttttaccatc aattgaaact tctttgccta
    31381 aaaagttaaa ttgaacattt gagatagtta ataaattctt tttttctcta ctttctcgcc
    31441 ttaaatgagc agttatccta gcccttaatt ctcctacacc aaaaggtttg gttatatagt
    31501 catctgcacc tataccaaga ccgtacataa tatcactttc catatttttt gctgtcaaaa
    31561 acagtattgg acaatcaacc ttatttctta tagttttaca tacttcaaaa ccatccatct
    31621 taggcatcat aacatctaat attattagat taaaataatt aaaatccatg ttcatagctt
    31681 tttctacatc actaattacc tttacttcat gtccatctct tttcaacaca ttttcaatca
    31741 actttaacat atctaactca tcatctacta ctaatatctt agacataata actcctccat
    31801 ttatccgttt aatagtattc taatttatat atttaataat atctttaaaa ttcacattaa
    31861 ccataatact ctcatattat agcttacaat aattaattac tattttacaa ataaaatatc
    31921 ttagatacaa aaactttata tttcaaaact ttatattcat tttacacgta tttacttaaa
    31981 tataaaaatt tatttaacaa tttattattg taatattttg aatatataat aaaattaata
    32041 ataagaaaac tttttataaa tatactttta attgtttaaa ttggaggaaa aaattatgga
    32101 tgaaaagata acaaaaaata attataatgt tccatttgac tatgttcgaa aaaaactcaa
    32161 agaattagac cctcatacaa ttagtaattt aagtaatata cctttcaata atgatttaaa
    32221 tagattcaca ctcaattttt ttgatagaca atatattata gactatccaa gtggagaagt
    32281 ttggaatagt aatggtagcc tttgcaataa ctttgagctt aaaatcttga tagttagata
    32341 tttaatcaat gctaaaggta ttcctagaac taataaatat gtaactttta aagaaatacc
    32401 aggaggcaat gtttattatc caaatttttt aaacaggact ctatataggc tttcagaagt
    32461 ttttacatct caaatagaca aatataagtt agttatggag aaaattggag ctgaaaaagt
    32521 aaacatggga gatttagctt ataggtttac atatatgggt aatacatcta tggtatttat
    32581 actttggttt ggagatgatg aatttcctcc aaattcaaat atattatttg attctaatat
    32641 agtttattac ttcaatgcag aagatttagc agttgttcct gatacagcta taaatgcaat
    32701 tttaaataaa ataaatcaat tataaatata aataatatta taataaagtt tttgatagat
    32761 ttaatagaat ataatatgaa atggagtaaa aaatgaatgt agaaaatcaa tctttatcag
    32821 atattgaaat aatattatca cacaggtatg ataatggtgc agactattgg actacacctg
    32881 ataagcgttt aataaaaggt tctcctttct ctactcttga tagtgtactt tatctattag
    32941 aattaggtgt gaaccctgat gatactttac ttaaagaatg tgctgacttg atatttagca
    33001 catggcgaga agatggacgt ttcaaactat atccaaatgg aggtatttac ccttgtcata
    33061 ctgcaaatgc tgctaacgta ctctgtcata tgggatatgc atctgacatt agaatgcaaa
    33121 aaacgtttca acatttttta gatacacagt atactgatgg tgggtggcgt tgcaataaat
    33181 ttagttttgg tcgaggtcca gaaactgaat actcaaatcc actaccaact cttaatatat
    33241 taaatgcatt ccgttttagt aattatctaa ataaagaatc aaaacttgac aaagctgtag
    33301 attttctact tgaccattgg actattaaaa aaccaatcgg cccttgtcac tatggaattg
    33361 gaaccttatt tatgcaagtt gaatatcctt tccgtaatta taacctattc ctttatgtct
    33421 acgttttatc attttataat tgtgcaaaaa aggataatag atttttagaa gcattgaaga
    33481 tacttgaatc taaaatgata ggtaataaaa ttgtcgttga acgtgtagtt ccaaaactat
    33541 ctaaattatc tttttgtaaa aaaggcgagt caagtgaaat agccacaaca cactattacg
    33601 aaatattaaa aaacctagaa aaataatagt tatatagata aaacatatta aaaaatagtg
    33661 gctttaaatt ttttaaagtc actattctat ataaatataa cattataaaa aatatattaa
    33721 actatttact cttttttaaa accgttgatt ttcttgctat ataagaaact attattatag
    33781 aaataactac tagagaacct ctatttataa agaaataatt ctttaattca ttaaatctaa
    33841 catctatatt gttaggcata taaaacatct tttgaatata aaatccattt acattaatca
    33901 tactaacaaa tgggtacata tgtaaaataa aaaatatatc agcaaatgta tatgtaatta
    33961 atattgctat tgttgtaaat ctattttttg ttaatgttaa tatcattagt ggaaatgttg
    34021 tataccataa taatgtagga aatgtcttta aaaacacatc tattggcttt acactataaa
    34081 caaaatctat tttgtctatg ttaaaacctt tcactgacgt aaatgtatat actattgtaa
    34141 ttgaaattaa taataataca gtatatatag ctaatataac acaccatcta aatactataa
    34201 attgattata ttttttttta ttatataaag taagtatctc atttatatta cccgaataat
    34261 cttttgaaaa cacataaatt attgtgattg ctgacattat tggtataaaa acatctgata
    34321 tctttataat tcctaaccat cctgctatag aatatgtatc cattataggt acatcattat
    34381 aaaatattaa ctcattataa tctaatatgc ctgtaataaa tatgcataag atagaaataa
    34441 ttacaaatat taacacatgt ttaagctcta tttttttaag ctcaaatttt attaactcca
    34501 atatacacat ttattttaac tccttatgta aaaagtttat ataagcatct tgtaaagttg
    34561 tatctatcct taagctatct ttaagtttct ctgtattttt acctatatac tttacttgca
    34621 caccatcttt aactctccta taatcaataa tatcaatatt acgttctaaa tattcaaatt
    34681 catcattaga aactactacc gaatacacct caaccatatc aataaactta ttaattgtac
    34741 catcatataa tattttgcct tgatttaata taattaattt gtcacagtta aattgtatgt
    34801 cttctactat gtgagttgaa attaatacta tagaattttt acttatttct ggtaacaatc
    34861 ttctaaattc attcctttgc tctggatcaa gaccaactgt aggttcgtcc ataactatta
    34921 acttagaatc acctattaaa gcctgtgcta tacctagtcg tctctttaca ccacctgagt
    34981 atgtacctat ttttttattt ataaacttgt ccagattaac ttttgatact actttttgta
    35041 tatgtttctt taaatcattc ttagatacac ctctcatttt agctacaaat tctaagtatt
    35101 cttgcccatt tatattatta tacatatcaa aatcttgagg tacatatcct acatttgcct
    35161 tgattttctc attgtttttg ctatacttta tattatttaa ttctatctct ccaattgaag
    35221 tttcataaag agtcactaat aacctcatta atgttgattt tccagcacca ttaggaccta
    35281 ataatgcaac tactccatct attagggtta aatttatatt atccaaagct tttttatttt
    35341 tgtaattaaa atttagattt cttatttcta acttgtttat catattaacc tcctttatta
    35401 tttatctatt ataaggatat ttcctgaaag ccaatgataa atagttatgc taattttctc
    35461 tccctttgag tcttcatata aaaattcatt gctaggtaag tcagcaacta atatcatatt
    35521 aatttttttt ctttttattg attccataac attatctact tcatctacat attttttagc
    35581 atctacagat gctttgctat cacctaatat ttcatttctt gtttcaatta aaaatttaat
    35641 tttttcctct atatccatat ttacattact atctgatgga tagattattt ttatatcatc
    35701 ttttacctct tctttaaact cacctgcata tattgcaata tcatttgact ttccttcaaa
    35761 tatataatct ttttttaaaa aattactagt cttattcagt aaaggtaaat ttgaatagac
    35821 attattttta gaacttaaat ttattttata agccatacaa cttgattgat ttacttttgg
    35881 gtaccacgct aaagaacctg gtggaagcat aatatcatct ttagtagcta tatatttatt
    35941 ttgattccaa gatgtcaaca cattaatata gccttcatac tctatatcta atgtcacctc
    36001 gtcattaggc tctaaacttt cctttaaatt aacttcaatt aaatcctttt ttttgttaaa
    36061 ttttatattt tccgaattta tttttatata ttttatatca aatatttcat ctaaaatcat
    36121 attaatattt gttaactctt catttgatgt gtttaataaa acaattctat cttttatatt
    36181 tgacttgctt ttaaagtcaa tcttcatatc atgcgatttt attttaatat cactaatatt
    36241 agatttcttt aaatactctc cataactacg ctcctcatct ctatatgttg acatatctat
    36301 tgatatataa tttaatccaa taaataaaat actgtacact actataacta ttaatgattt
    36361 aatctgtttc tttctaatat catttaatat cagatatgct actattatta aagtaattgc
    36421 aaatagtgta ggtataatct tatctataac atatgctgaa ttattaactt tccctacaaa
    36481 atcagaatag taagcatatg attgatcttc aaatatgtta agtgatttaa ataatgcttt
    36541 aaaaaatggt tctttaatat caataactct atccaacata ttagttgatg ttaaatataa
    36601 aaatactgat gataaggtaa tctttagtat taaattattt ataataactg aaataaatga
    36661 tatcactata gataaaatag taagtggtaa aaaccattca aatatcaata taaatatagt
    36721 tgatttaatc gttacaattg acaatgttct tattaaactt gagtatgcta tagcatatat
    36781 aattggtatg caacaaaata taaagttgtt tctagttaca gcaacagaaa tacctaaaaa
    36841 ataatcaaaa ttatttctac ataatatttt tacagtatca taactttttc tcatataaaa
    36901 aattacattt aacatggtta aaatactaat tgtcatacaa gtatatacac tcaaggataa
    36961 gtaatcatta aatgaaattc ctctattgga tatatgtgtc catcgttgta tgagatggta
    37021 atttataata ataattgtta ttgcaaataa aatatttttt ctaattattt tggttataaa
    37081 gtctgatata gccttaatac tactcatatt tatgcccctc ctagccagtc ttttgataca
    37141 acctacaata gattcaacta tatttaataa gttcaaatac ttaccttgac attatttttt
    37201 taaagttaat attaattagt gaagttaatt aatttagaaa atgttttata atcttactga
    37261 tcactattaa ccttcattcc aatatatatt aatatagcaa tccaatatat agaaattatt
    37321 gcataaatca catattgctg aaaaatatta agtattttac ataaaataat taaacatatt
    37381 attacgaata gtattttttg cacagtcttt ctataatgct ttttctcact ttcactaaga
    37441 ggtttgtttc tatgttctaa aggagctaac ttgtaaattg ttagtatact aataatcatt
    37501 aataatacta ataaatactt atatttattt atatctatat tattagctga aaaaatagtt
    37561 atcaaataaa taactgcaaa tgttaatgta cactctttat aattcctagc atgataacct
    37621 cctgtgaact gtcttaaagt gcaataacaa cttaaaaata taactgtatg tataaatcta
    37681 tcaaaaataa ttcctataaa taatgctacc aaaatattaa ctatgaaata aatcaaagtt
    37741 tcaaatccat atctatatat ttcaaaatct tcactttcta caatctcatt attaactaat
    37801 atattggcaa atttatatga taatctttta aacatatttc ctcctcaaca tgtaaaacct
    37861 ctcctttaat aagaagaggt ccttttatta atgttatttt tttagttttt gtaaagcttg
    37921 tggctctttt gcctgatgtg ctacccaaga acttgcactg tttgctgata atacagctat
    37981 cccaaaactt aatgctgaga tgtttttaag taagtttaac gctatttttt tcattttgta
    38041 ctctcctaac tttttattgg tatcattatt tttaatataa actcattatc tgagtaatta
    38101 actattgttt caccaccata tttatttact atatatttaa tactagcaag tcctattcca
    38161 tgtatcttat catcatcctt actagtttga atacgtttat caataagttt aatatcattt
    38221 accttagtat ttataaattt tattattgca aatttattta tatatgtagc tttaacttct
    38281 attctttttt ctatctcatt atttatatcc atacatgctt caattgcatt atctagtgaa
    38341 tttgcaaata ttgaacatat atcattattt ttaataaagt ttaatttaga tatatttatg
    38401 ttatcttcaa tttgaatatc atacttttta caaatactta ttttttctgc taaaattaaa
    38461 tctaatgttt tatttccagt atttttaaaa tgttcaaatt cacttatctg aaactctaag
    38521 ttatttatat atgatataat ttcttcttta gtgtcataat tttttataca aatcatatgg
    38581 tttttcaagt catgataaac atattttaat ctatcatgaa cttcgtttat attttcataa
    38641 ttcctgtagt ttgtttttat tctttcattt ataagttcat actctagatt taatttatcg
    38701 tcttgtacaa ttttccctat tactattaaa agtattatac ttgatgataa aatcaataaa
    38761 attgcaaata taaatataaa taagttttct gtagtaattg tattgactct taaattatat
    38821 tcaaatataa gtaataagct aactatatta gataatatag gtattcctat tagtatcata
    38881 tctttaggtt taaaatctaa agataattta aaatacttaa acaatataaa acctataaat
    38941 aaaaatacct ttgacataat aatagcttgt attctaaata agttttcatg ttgtgttatg
    39001 ttaatattat ttaatttatt tataaatact actagaccta ctgcagaacc ttcagcaacc
    39061 attaatccta gccaaaacaa taaactaact attatacttt tatagatttt atcttcatat
    39121 gatgttatat aaaatgatat tcctattacc atacatagaa gaatcttaat atttggaaac
    39181 actttaacta catttatgta tgccataaat ataattataa ttattgttgt aaatatatat
    39241 cttatattag atattttcct ctttccaact aagtggatta ctaaaaaaaa tataatccaa
    39301 tctatagtac ctgaaactaa attaataata ttccaaaatc ctacacttaa atttgttagc
    39361 atactatgtc ccctaatata ttagttatcg caagcttcaa gcctttaacc ctatatttac
    39421 ttattggaat actttttcca tctactataa cagtacttcc actcattgat tctactaatt
    39481 ttaagttaac tatatagcta ttatgacatc taaaaaatcc atattcattt aatatctttt
    39541 ctattttaga tatactcatt ttagtaatat acatatcatc atgtgtataa atcaatatat
    39601 tcggtctatc tgtttctata taagtaatag aatctatttt aattcggtct acataatttt
    39661 ttacatttat agttaagtaa ttatttctct ttttcatcat ctcatttata caaggtaata
    39721 cacttttaga aattttttct ttatttattg gctttagtat atatctgtat gcttttacct
    39781 cataaccttc ttgcataaac tctacaaatg aagtaacaaa tattatttct aacttatgat
    39841 caaactctct tatcttccta gcagtatcca taccatttat tgttttcatt tgtatgtcca
    39901 taattaatat gtctaaatct ttaggatagt tatttagtaa ctcttctcct gctgaaaatt
    39961 catatatgtt ataatttata ggatatgaac ttaatatttc acttaatata tcttttattt
    40021 ttattcgata atgtagttca tcatcacata ttcctatatt aatcacaatt cttaaacatc
    40081 ctttcttata tatttttaat aaaaatatat aaatttctat tcattttaca tattttaaca
    40141 tacttctgta gtaaatagct atttagattt taattttagc tttttggaca ttaagtatta
    40201 ctttttggat ttgcaatcta ttttgatttt cattataata caatttttta atatatatta
    40261 aatatattta gggattttaa taaaatattc ctcgaaaagt ttttaatatc tagtttttgt
    40321 taaataaaaa gtatgcacct tcctttttat agtaatattt taattatcgc tataattttg
    40381 aagaatacac actatatttt aaatattaat ataaaacata ttaaaaaata gtggctttaa
    40441 attttttaaa gccactattt ttttaaatga tgttcctgaa aatatattaa gttagttatt
    40501 ctaatcaatt tgaagacttt tgcttctcct tgcctatatc taacataatt tcataattta
    40561 atttaccttt ctttaaatca actcctgagt tacttctaat aatcccacaa tccaacattt
    40621 cgtcaaatac cttcctagat aaacctagtt tctcacgtaa taatgttgat acctttaact
    40681 gacatggata tttactaata atatgaactt gtgtaaggtc attaatatta atttcttgtc
    40741 ctactatttt atactctgga ataccaactt ctgctccatt tctatgtaaa agttccatat
    40801 tcatagcata ttgcttaaca agattataat cgtttgtatg gaattgttct agtatttctg
    40861 gatttaagct ttttggatta acacgagaat atatagttga gttccatgtt gaatcacaat
    40921 ttgaacactt gtatattaac catatatcca aatatttatg ttgagcatta accctaaata
    40981 aacctgaaca aatatattca gtcttttttc cacatttttt acaataccta attacaggta
    41041 actgggagat atattccacc tcccaagtaa tttttttcat aaatgcaatt tcctttctat
    41101 aagattacta aattaataga aagttatgaa aaggtggaat ataatcaagt atttttttca
    41161 aaacaagacc tcctaatttt aataaatttt aatttcaaac atataaaaag ctactacttc
    41221 ttattagata ctttacaacc agctttgtta gctactgtat aagttattga aattacttaa
    41281 attatatcat ccacttaaaa gaaactatac ctcaaacact tttatcaaaa gaaaatgagc
    41341 cagtataatt ataaaactat actggctcat ccttagatat cttttctttt ttcttgtaat
    41401 acaattcttt ggatactttt caaagataaa taatactttt ccgataaata ttgcaaatca
    41461 tatccaactt gatagtcttc gtaaatctgc atatttctaa ttgacaactc ctcacgagta
    41521 ggtgtattac ttccccattc ttttttatta ttagattttc taggaatata gatacattca
    41581 ccatctacat attcctgtat taattcaagt aattcttctg gtaatatatg tattgctttt
    41641 ttatagctca ttttgctctc ctattaaata gtaatttttc attaggaata gcaaagggct
    41701 atttatatat taaatttcat agttgcaata gcccttgcta tgcaaaaata aagattaaat
    41761 caaacataaa atctcaaccc tccttatata tcacagcgat acatataagt ctatcaacag
    41821 tataactggt tgtcaatcaa cacaaattat ttatccattt tttaaacaca tttatttagt
    41881 ttttatatta tatttatttt gataactaaa attttttcat tataaactat ctttaaaatt
    41941 attagtcaac ttaaatccat tatcttgatg ggtttattac tgaacaagga actccaacta
    42001 tacactttcc acaaatatca catccaccta tataagtgta ttttttttca tttatcatac
    42061 acatttcata acacttattc ctattaaatc catcttcttt taaagcttct ttcacgcatt
    42121 tgttgacaca ttttttgcaa ctattattat gtttatacaa gcagtattca ttttctggtc
    42181 ttttagttgg ctctaattct aaacttgtga ttatgcttcc tattcttcca caacaccctt
    42241 tttctgtaat aagcatatta tttaaaccaa aagttccaag acctgctatg aatgctacat
    42301 gtctttgtga ccagtcactt accaattttt cactgtcaaa attatacgac ccaggaatag
    42361 catatgaatt agcccccatc ttctctagtt cttcacataa gaaatcattt aaatttgaaa
    42421 tcaacatatt agtttctatg tacgctcttg cccatatttc agaacattca aaccctccaa
    42481 tattactatt atttacactt tcatcaaaag gtataaaata tgtaattata gtttttccat
    42541 tttccagaaa atcttttggc atagcatgag atgttcctac aacacttttt agcttctgaa
    42601 acatatcatc ttcagcagat gcataagcaa acaaaagttc ttgccacttt gtagttatat
    42661 tatttgattt atgataattt tttacaaagt tgttcattga tatttcaatc ttatttttca
    42721 tagtatcacc cctagataat tctatatttc caatttaatg ttattatttt atattatata
    42781 atagtatttt ataatatgcg cactaattaa tattaaaact atgttcatca atcattctta
    42841 aaaaagctcc taatatcaat acgatattag aagccttttc ttatctaatc taatttattg
    42901 caattcctgc acttgcatat cctgctacag tattaactaa tgaaagacct gaagctgttg
    42961 cagtaaatac tagtaagaat cgagtttctg ctgttgctgc tatatttaat cctgttacaa
    43021 ttccactaga aattgaacca actgataata ttcctgtaag tggtggagct agtgtaactg
    43081 tcgctcctgg tacagctgta aatgagttat ttggtgcagt agattggtaa agtgttgctg
    43141 taattgtaac tgttgagcca acaagtgaaa gtgctgctgt tgtactgaag tatgctgaaa
    43201 tagatgttat tgttccagct cttggcattg aaaatgcaaa gttagtcaat gttcctgctg
    43261 cgtttgtaag gtctattact ccaccaacta tacttaatcc tggagctgaa ctaccaaagc
    43321 caacaaaacc tggtgtacct actaatcctc cagctatagt tgtaagtgat agtggtatac
    43381 ctgatgcaaa aggtattatt gcactagcac ctgttggacc tgttgctcct gttgctcctg
    43441 ttgctcctgt tggacctgtt gctccggtcg ctccggttgc tccggttgct cctgttggac
    43501 ctactaatcc atctgcccct gttggacctg ttgctcctgt tgcccctgtt gctccatctg
    43561 ctcctgttgg acctgttgct cctattgggc ctgttgctcc tgttgctcct gctaatcctg
    43621 ttgctcctgt tgctcctgtt ggacctacta atccgtctgc tcctgttgga cctgttgccc
    43681 ctgttggacc tgttgctcca tctgctcctg tatttcctgt tgctcctgtt gctcctgttg
    43741 gacctgctac tccatctgct cctgttgctc ccgtcgctcc tgttggacct actaatccat
    43801 ctgcccctgt tggacctgtc gctcctgttg ctcctgttgc tcctgttatt cctactcctg
    43861 ttgcccctgt tggacctgtt gggcctacta atccatctgc tcctgttgct cctgttgctc
    43921 ctgttggacc tactaatcca tctgctcctg ttggacctgt tgctcctgtt gcccctgtcg
    43981 ctccggttgc tccatctgct ccggttgctc ctgttgcccc ggttggtcct attagtcctg
    44041 ctgctcctgt tgcccctgtt actcctgttg ggcctgttgc tcctgttgct cccgttgctc
    44101 cggttggacc tattaaccct gttgctcctg ttattcctat tcctgttgct cctgttggac
    44161 ctgttgggcc tgtcactcca aatcctgttg ctcctgttgc ccctgttgga cctgttactc
    44221 catctgctcc tgttgcccct gttgcccctg ttggacctgt tactcctgtt gctccgtctg
    44281 ctcctgttgc tcctgttgga cctgttggac ctgttattcc tattcctgtt gctcctactg
    44341 ctcctgttgg gcctgtcgct ccaggtgttc ctgttgctcc tgttggacct attaatcctg
    44401 ttgctcctgt tactcctact cctgttgctc ctgttgggcc tgttggacct gttactccaa
    44461 atcctgttgc ccctgttgct cctgttggac ctgttattcc atctgctcct gttgctcccg
    44521 ttggacctgt tggacctgtt actccatctg ctcctgttgc tcctgtcgct cctgttgggc
    44581 ctgttggacc tgttactcca aatcctatag ctcctgtcgc tcctgttggg cctgttgccc
    44641 ctgttgctcc tgttggacct cttaatcctg ttgctcctgt attccctgta tttcctgttg
    44701 gaccagtcgg accggttggt cctgttgggc ctgttgcccc tacacctgga cctgttggac
    44761 ccgttggacc tgttgggccg gttctacctc ttggacccat tgggcctgtt ggacctacac
    44821 atgatggtgg acagcaatga tggcaatcac agctattata atcatcacga ccattgttac
    44881 aatcatcata tttatcatag ccattgttgt aatcattatc atcaaatggt ccaaaatatt
    44941 tatttctact cattattaaa agcacctcct gatatatttg agcttttaag ctctatatat
    45001 aatatgtcaa atttagtatg attgtgaccc atcaactatt tttaaattta ctaaaataat
    45061 tttcattgtg caaaacagca ctatcattag gtttgatttc ttttgttttt tcatgataat
    45121 aaatagcttt atctgattct cctaacctgt cataacagac acttaattgt atagatggta
    45181 tatatccata acaatcatct gacttaaagc ctccatcagt atcattctta tctcttgtta
    45241 atgctacctt ataccaaaaa atggcttctt tatatttttg tctatcaaac atatgctttc
    45301 ctatgtcaca acaaatttct gctcttggct catcaaattc gaaacttcta aacaatgaat
    45361 ataaggcact tttttcatca ttaattaagt aataacatgt ggctaaatct ctgcaagcac
    45421 tgatacaatc ctcaatccat cctcttgatg agtctaaaaa cttagtaaaa ccttctattg
    45481 cttctttata cctagcatta tagtacaact ctctagaata atagaattgg tgtcttggct
    45541 ccaatgtttt tccttctgaa atcatttttt caaaaattct aagatttctt agagggtcct
    45601 gtctatgaag ctttttatga gatattgcaa tgtcagaata aaatacctct ccaaaaagag
    45661 gtactacttc atgaataggg tcaacccatt tatgattaga acttctttta aacaatcgtt
    45721 ctctataata tgagagagtt gggttaccat tttcatcaaa ggatacattg tattttgcca
    45781 taacaatatc aatggaagta tccaagcttt ctttaatctt taataatttt tctctatcag
    45841 cttctaaaat tatgtcatct gcatcaagcc acatagtata gtctttagaa gctttagaaa
    45901 atgaaaaatt tcttgcagca gagaaatcat ctatccattc gaaatcgtac accatgtctg
    45961 tatattttga gactatttct tttgtggaat ctgtagaacc ggtatctaca attataattt
    46021 cgtctactat atcctttaca cattctaagc atctgcctat aacttcttcc tcattcttaa
    46081 caatcatgca caaacttatt gtaatcataa tgtacaactc ctttgtcttc tatatattgc
    46141 agacataagt aatatatggt attttctctt gttttgttat taaatttata aaaacttttg
    46201 acgacagaaa gttactaaag aaccttatct tttatgtatt ttttatagta gaatttatta
    46261 aacttgaaat ttggtaataa ttgtcgaaat gataaaacca tatttaattt catacaaaaa
    46321 agagtgtccc ataaactaaa aagttcattt tgagacactc ttatttaata ttatatatat
    46381 atttgtaaaa cagacatttt aatatgatac tttgatactt tattcattcc tattcattat
    46441 ataatcaatc agcccgtact ctttagcctc tagtgcagtc ataaaattat ctctatctgt
    46501 atctctccta atatcttcta ttgatttacc tgtattttca gaaagaattt tatctaatcg
    46561 ttcttttatt ttcaatatat tatctacagc tatttttaca tctgttgctt gacctttagc
    46621 tcctcccatt ggctgatgaa tcataatctc tgaattgggt aaagcacatc tttttccatg
    46681 tgttcctcct gcaagtaaaa aagcactcat gcttgcagcc ataccaatac aaatagtcga
    46741 tacatcacat ctgacataat tcatagtatc atatatagca aatcctgatg tagcagagcc
    46801 accagggcta tttatatata taattatatc tgaatctggg tcttctgatt ctaaaaacag
    46861 taattgagct actactaaac ttgcaatagc atcattaatt tcttctccta taaatataat
    46921 tctatctttt aataattttg aatatatatc ataagacctt tccccttgac ctgtttgttc
    46981 aattacatgt ggcactaaac tcatgctact ttcacctcac aattaatatt aaagttcaga
    47041 taagaattga tttcatctct tctttgttta taattcatat ttgcataaga aatacatttt
    47101 tcactttgaa tttgatgatt tagatttaaa acaaatctct gttgaatagg ataaaattca
    47161 tctttatttc tatatacttg tggagattct ttatataaat ttctaaatgc tagtgtaaaa
    47221 gattgatgtg tagaatatcc tactgtaata gctatgtcta taatatattt gtctgtaaat
    47281 ataagttgtc tagctgcttc agtaagtctc ctcctctgta tgtattgatg gatagtacat
    47341 cctacaacat ttgtaaacat tctgtgtaaa tggtattttg aataattagc aatataagct
    47401 attttatcta aatttagatt ttctgtaaga ttttcttcta tataatcaat tatctccata
    47461 ataacatctg tatttgtttt caactctatc ctctcctttt aagtattata acctatgcaa
    47521 aaagtttttt cttaatataa attgctatct taatttagtg tatagcaata aagtatcgat
    47581 aaaaaaatat caggtatttt tatacaaaaa tattaatata tatttctttt aaagtttaaa
    47641 cttaattaca tctattatat gtagattgct acatgtttta tctatgtacc ttgattcata
    47701 tgtattattt tgctgagtat ttttacacct ttttcaattt catcaaaact aagatgagca
    47761 taacctagta taatttcata gtcatattct aatatattct caattgcata ttgttgtata
    47821 ggataaactt ttatacctgc ttcaaaaatt ttatgaatta aactttccgt aaatttaaca
    47881 ttatagaaat gagcaactat atgaagccct gctgcttcac cttttatttt aaattcattt
    47941 ttaaattccg tgtttaaaac ttttatgagg tacttacgtt ttctattgta caacttcttc
    48001 attctaaata tgtacttctc taaacctcca tttccaataa attcagctaa tacatgttgc
    48061 aatattgttt ctgtatgaac atcagagtac atttttaagg ttttatagct ttctaaaata
    48121 ttgtctggta ataacataaa tccaacacga atagctggag ataatatttt actaaaagaa
    48181 cctatgtaaa ttactttatt tggactaagt tcataaagag aactaattgg ttgaccctca
    48241 tatctaaatt cactatcata atcatcttca attatatagt aatcattctc tgttgcacat
    48301 ttaataagct ctagtcttct ttgaataggt aaaatccctc ctaatgggta ttgatgagaa
    48361 ggtgtagtat aaacaaaaga aatccccttt attgatttta ttaagttggt attcattccc
    48421 ttatcatcta ctttaattcc aacaactgaa tatccaatag atgaaataac ctttaataag
    48481 cctttatgaa tagggtcttc tacagcaacc tttttattac ttttgtataa aagctgtgaa
    48541 attaaagata aaccttgtgt tgaaccagaa acaatcataa tgtttttggg attacatcta
    48601 attcctctag tacgaaataa atacttagat atttccactc ttaaaaccca tacaccagct
    48661 gtacttccat atctgaaatc cgaatctgat aaatcataca gtatttgttt atatagcttt
    48721 gaaagttctt tgcgtggaaa tagttttaat tctggaactc ctgaacgaaa atctatataa
    48781 tttttttgtt tattagtatg taaatctttt tcatctctaa aagtatcatt taacttaaaa
    48841 tcaactttat gccctgatat tcctttagct acaattgttc catatccatt ccttgtttcc
    48901 aaatatcctt ctgcaattaa ttgattatat gcatctaaaa ctgtattacg agatacagat
    48961 agttcttttg ataaagtcct agatgatggc agtttttgct caaactttaa gcttccatcc
    49021 agaatcatat cttttatctc acaataaatt tgtcttgata atgatatatt actatttcta
    49081 ttaatcgtaa tccacattct aatacctcca aaagtggttc tataaaatta ctataaaaat
    49141 ggaactacaa aatccacttt taatttgcta agattatatc ataaaatata aaataaaaag
    49201 gagtctatat tatgttagaa atatcacata aagttaaaga aatttatccc aatattaaat
    49261 ttggagttat ggttataaat attacacatt caaaacctaa taaagaaaat tttttagttc
    49321 ttaaaaactc tacaattaaa aatattatag aacaacaccc agaatacaat cgtaaagaaa
    49381 aaattaaaac agagcctgtt tcaagttata taaagtatta taaaaaattc aagaaaactt
    49441 accctgtatt gcttcaatta gaatctattc tattaaaatc aaaaggtatc ccagatgtag
    49501 gtgtaacaat tgagtcaatg tttttagcag aattgaaaaa tcttcttcta acagcaggtc
    49561 atgaccttga taaaatggaa ttacctttga aaatagaatt agcaaatgga agtgaacatt
    49621 tttatggtat aggtggtaaa gaacatattt taacaaaaga tgatttattt ttatcagata
    49681 atattggtat attatctagt attcttaatg gccctgataa tcgaacagct ataacaaaag
    49741 atactaaaaa tattatgtat tttgtatatg gacctgataa aatatcagaa aagcaaattc
    49801 aagaccattt aaatgacata aaacactata tttcaagtgc ttttccagat ttaaaagtta
    49861 attcaattga tatattttag ttaatatatt aaaatcctca taaatatttt gaataaataa
    49921 attagtgaag tatacattct agttaaacta gatatacttc actaaaatta attatatgta
    49981 gatattattt attttgaatt ttgtttataa taaacacttt attacattta tcctatgaat
    50041 aacttaattt tataattaaa gagtttcata agaaacaatt tcacttaaag gaacacgagt
    50101 tttttcatgt ctctctgggc tctctggtat cttagattca tatcccatca ataatatatt
    50161 tattggttct agattatctg gcaaactaaa ctcttctcta attatatctg gattaaaata
    50221 gcacacccaa acacttgcca atccaagttc agttgcctgt aacatcatat ggtctgtaac
    50281 tatacttgtg tcaatatctg taagttgttt cccatcaaaa ggtcttgtcc aaactttgtc
    50341 tttatctcca catacaagaa ttgctaatgg agcatcatat atattggcag ccttagaaag
    50401 tttatttata ccttcttttt cctgaataac aatcaatctt tgaggttgac gattaccacc
    50461 agttggagct attcttgcaa catctaaaac cttctcaagt ttttcttttt caacctttct
    50521 atcttgataa ttacggcaag aatatctttt ttttgcaagt tcaacgaaat tcatttattt
    50581 acctcctatt gttttattag gctaagttga tgatttattt aacctatgct ataattttat
    50641 cactatggta ctatattaca ataatgcact tttttgtaat atagtaccta aaaagatact
    50701 ttaaaataaa taaaggagcc caaaaatgaa aaaatgttta gataattaca gttgcccaat
    50761 agaagcaaca cttgcattga ttggtggtaa atataagaca cttatacttt ggcatcttaa
    50821 agacactata ttgaggttca atgaattaaa aaaattaatt cctaaagcaa cgcctaagat
    50881 gttaactcaa caattacgtg aattagaatc agatggttta attataagag tggtttatcc
    50941 tgttgttcct ccaaaagtag aatactcgct atctgatttt ggaaagagta ttattccaat
    51001 tttagattcc atgtgtgact ggggttctga ttacttagaa aatctataaa ccattacagt
    51061 ttaaatccca ttgctaattt aacttcatcc atttgttctt tagatgtcaa catttctaaa
    51121 agcttaaaag ctacatgtgg agctgtttct ggacaatatg aggttataat atttttatct
    51181 acaacaattg gttcattaac tacatttaca tcaaactcac ttaactgtct ttgtctttta
    51241 ccatttttca aatgatatgt agtagcttta cgatttttta aaacaccact ttttccaact
    51301 ggcaatgctg ccacacaaat agaagcgatt attttttctt ttgaattaaa ctcccttatt
    51361 agatttaaaa aagatgaatc atatgcttca tcataaaatc caaattcttc aaatccccca
    51421 ggaatagcta gagcatcata atcatcaaca catacttctt ctatcgtctt atcaactaat
    51481 acttgtatat taaatgtgct cataacttgt tttttaaatc cacaagtcac tacatcaata
    51541 tcatgcccat aatcgttacg agcccatccc ataacatcta caaatacact aaattccata
    51601 gtttcaaaac cttttgctaa aaaaactaaa actttcatat tctattttcc tcctaaaata
    51661 taattaacac tttactagta aattatttaa aaaatatctt ttattgtatt aaattcttct
    51721 ttaattgtag ataaaacaat ggttgtatta gctttttgaa tccccccaat ttccttcaaa
    51781 gtcactgata taaactcttc aagattactt gtatcttctg ctaatacttt tattaagtaa
    51841 tcatactctc ctgaaatatg atgacattct aatacagtat catattccaa tatagttttc
    51901 ttaaattctt tcatactttt agataaatca gtattgataa atataaaagc caaaagctta
    51961 tattttgttt tttcccgatt aatttgaata gtgtactttt gaattaaatt tgactgctca
    52021 attttcctta tacgctctga gactgctggt actgataaat taatttgtct gcttatttca
    52081 gaggttgaag ccctactatt cttttttaga gaattaatta tttttaaatc tatttcatcc
    52141 agagctactc acctccttaa ttatttaata caattttagc taataattta cattaagtaa
    52201 ataaatttaa tcatttcaat aaaatttaat acaaatatgt tatttttcct taaaatttta
    52261 gttttcttta atcaaaacac atataatact taaaattatt taagttgaat taatgttttt
    52321 attttgtata atatattaga accttaatat atcattgaaa attcaacaat aatagacata
    52381 tacttctaca tgttgtaata tttatgaaaa tagtatccct atatcaaaat tataaaactc
    52441 ataatcttaa ttttcttata gactataaaa ttttattgat agcgagtctt caaataactt
    52501 tgtaatgata gatttactaa attaatataa taatagtaaa tataactttc ttattgacta
    52561 taactaagta ttatgataac ataacttaag ctataattaa taaaacatat tttaaataaa
    52621 taagataatc cacaaagtac aaaagtacaa tttaaataca gataaactag aattaatact
    52681 aagattagga tgtgaataat tatgatttat atagaattag ttaaacttgt tagaaaactt
    52741 aactctacac aaatgtatac gatgaagcgt tgtgtagagg ttaatctgaa agcttaaagc
    52801 tttctatctt catcggcaaa tataggttta gtatagtttt ttggctatgc ttatttaaaa
    52861 ttacctattt gccgatagat aatatagatt ttctggtcat agacaacaat gcttgtctat
    52921 gaccttttgt ttttccaaaa gcagaggtcg gcattttaca atgccattcc tctgcttttt
    52981 ttatttccat aaatttttta ataaaagaaa aggagtttga attatgagtt tattaaaagt
    53041 aactaattta tcacaatgtt ttatggataa atcactgtat gagaaagcta actttgattt
    53101 attcaaaggt gaacatatag gtgtagttgg ccaaaatggt acaggtaaaa gtacattaat
    53161 taaaatttta ttgggagaag ttgttccaga ttcaggagaa atcaagtggc aacctaatat
    53221 caatataggc catttagacc aatatgcaga aatcaataga gatacaacaa tatcactcta
    53281 tttacacact gcatttgaag aactttataa aatagaaaag gaaatgaatt tactctatca
    53341 aaaaagtgct atatctgaaa atgagcaata tctaatcaaa gcatcagatt atcaggaaca
    53401 actaatagca agtaatttct attctataga taatgaaata aataaaatag caaatggtct
    53461 tggattagat tcaataggaa tgaatcgtgt tgttggagag cttagtggtg gtcaaagggc
    53521 aaaggtaatt ttagcaaaat tactactttc taaccatgat gttctattat tagatgaacc
    53581 aacaaacttt ttagataaag aacatgttga atggctctca aattatttaa atacatttaa
    53641 tggggcattt attgtagttt cccacgattt tgacttttta gaaaaaattt ctacaggtat
    53701 tcttgatata gaatttggca tgattaagaa atatcatgga aaatattcag agttccttaa
    53761 acaaaaatca catttaagag aagattatat tagaaggtat caagcacaac aaaagaaaat
    53821 agaaaaagaa gaaacattta tacgtaaaaa taaagctgga gtaaattcaa aaattgcaag
    53881 aggaagacaa aaacaattgg ataaaataga aagaatagct cctcctagtt ttacaggaaa
    53941 accaaatatt caattttcag aaattgaaat atcggctcaa aatgctctta ctataacaaa
    54001 tcttgaagtt ggatactact attctttact tccaaaatta aatttctctg ttgatggagg
    54061 tcaaaaaata gttataactg gttttaatgg aatcggtaaa tctaccttgt taaagacatt
    54121 agtcaaagat attcctcgta tatctggaga tttccagttt tcagaacaag ttaaaattgg
    54181 ttattatgag caagatttaa aatgggaaaa tcctgataaa accccacttc aaatagtagc
    54241 agataaatat ccaaaattaa atacaaaaga gattagacgt catctagcca gatgtggtgt
    54301 gaaggaagag catgtttcaa ggagtgtatc tactttaagt ggtggtgaac aatctaaggt
    54361 taaattatgt tgtatgatgt tatctccttg taattttctt atcctggatg aaccaaccaa
    54421 tcactttgat gcagagacaa aggatgcttt acaaaatgct ttgaaacagt ttagaggaag
    54481 tataatccta gtttcccatg aagaaaaatt ttataaaggt tggattgata aagtatttaa
    54541 tatagaaaaa caattagtat aagtttatac acaatataca atattattta attagtattt
    54601 caatgcaaat tttaatgttc tagttaatgt ttcactgcaa ataaactaaa aaaattttat
    54661 tagaagataa agtacattac caggatataa acttaatgag gctatctcaa aatagagata
    54721 gacctcttct tcattgttga gataacgaaa atatatttga ttttacccat aaaagaatgc
    54781 ctcattaact aaaaattcaa cttgagatat cttcattaat attaaaatct attctaatcg
    54841 attctattca caagtttata tcctagtcct ttaactgtta taagtatttt aggatttgag
    54901 gggttatctt caattttttc tcttatatgt cgtatatgca ccataattgt attgtcacaa
    54961 atactactat atactcccca tacttgttca tagagtcttt cttttcctat aattttattt
    55021 acattttgag ccatgtagct taaaagatga tactcccttc cagttaactc aatctcattt
    55081 ctatctttat atacacggtt tccttctata tcaatagtaa tatctcctat tttaataact
    55141 tcactatctg atggtacaat gctttgatac tgttgtctac gtaattgcgc tttaactcga
    55201 aaagcaattt ctcttggact gaatggcttt gttatatagt catctccccc actactaaga
    55261 cctagtattt tgtcaatatc atcatttttt gatgataaaa aaagaataga acagtatgaa
    55321 aactctcgaa tttttttaca cacttctata ccatctatat ctggtaacat tatatcaaga
    55381 atgacaacat ctggacatgc tatcttacac agcgaaatag catctagacc attataagct
    55441 ttaataatgt ttttaaaacc atcatttatg agtacttctt caattaaatc tactatatcc
    55501 ttttcatcat caacaagtaa tattttttta ttaaaacatg tatccatatt gaaatctctc
    55561 cataaaaatt tattatattc taatcttgca gtatcactat cttgtttttt atattcacta
    55621 ctcttgcttt tacattacta atagtaacat ctctaataga caaattcaat cactatgaaa
    55681 attaagataa gtttctttaa aataaagtgt atgatacatt aaaacctata gaaaattaat
    55741 ttcatccaaa gtttataata ttagactaat acacttacag atacagatta atacaatagt
    55801 aaaataaaag gagttgtctt aaaattgact ttttctaatc aaactttaag ttcaactccc
    55861 ttttaaactt ttatatgtta tttttattaa ttctttatat caattataac tttaattttc
    55921 aatagtatat atctcccact aattgaatta cttattaaaa cataaaatat ctacctaaac
    55981 tatttattta atatctacat atatttatat ctattcttat acttacatat acaaaaaatg
    56041 gttaaaaata aagttaaaat ctcagcactt ggtactgcta accatatccc atttatattt
    56101 aatatatttg gcaatatcaa taaacctata gttataaatc caaatgtacg tagaaaagat
    56161 aatattgcag acacctttcc atttgataat gctgtaaaca tagacgaagt aaaaatatta
    56221 tacccacaaa aaataaaact aaaagaaaat atataaaatc cagaccttgc aatttcataa
    56281 actcggcttc ctctttgaga aaaaatctct acaatgattg gtgaacatga tatagaaaag
    56341 ataaatacca ataaagatgc tactgaaata aataatatac aaatcttaaa aacatgattt
    56401 aactgcgtat tattttcact tccatagtta taacttatta ttggtgcaac acccattgag
    56461 aaacctatat aaagcgaagt taaaagaaat tgagaataaa taattattgt aatagctgca
    56521 accccatctt caccaattaa tttcatcatc atagcattaa ataaaaaagt agtaactgct
    56581 gttgatagtt gacccaccat ttcagatgaa ccattaaaac aagcttcaaa taatattctt
    56641 ttatctagtc taggtataca aaatttaagt aaccctttat tttttgaaaa gaaaaccata
    56701 cctgaaacgg taggaattag gtatcctact ccagttgcaa gtgctgctcc actaataccc
    56761 atttgtaaca gaacaatgaa cacatagtca agaattatat tagcacaccc agcacctaac
    56821 actagccata atccaaattg aggctttcca gctgttacaa ataaacattg aaataatact
    56881 tgtagtatat ttgcagaagc aaaaaccaag ataataaaaa gatatgaacg acaatattta
    56941 attaaaactt cacttgctcc taaccatata atgatataat ccataaatac aatacctata
    57001 ccagtaatta ttaccccaat tacaactcct gataatacga ttaaagaaaa agctttattt
    57061 gctccctcaa tatcaccatt tcccatctta cgtgctacaa tagcacttcc tccagtggca
    57121 agcatagttc caattccaac tataagatta ataattggac atacaatatt aattgctgat
    57181 agagcatttg tattaataaa tcttgataca aatatagtat ctacgattgt atacaacccc
    57241 ataaaaatca tcataaaaat agtaggaaat gcaaacttca atagagtaaa taaattaaat
    57301 ctttgtgcca gtaaattatc ttgctcataa agattatctt gttcatgatt ttctgaataa
    57361 tatacatcac tcattttgtt tatttttctg tacattaatt atttttaaaa tatattccaa
    57421 acaattagac atttacagac ctcctttctg cataagaatt atatctaatt atttggatat
    57481 agttttaatt aatcttgctt ttttacacaa ttatatttct actaaagaaa cttttaaatc
    57541 cttactttaa taattttata gctttcagtg cctttagcgc aaatacttgt tcacatagtt
    57601 tagtgtcata tgaaacgtat ttatatatag ttatacattg tctattttta ctaatttatt
    57661 aactacttgc acttttataa tgtctcatac ctaacttcca aaatctccac ataaacaatg
    57721 taaacaaaaa tacaattaaa attgagtata ttaatccatt aattgttatt gtcccagcca
    57781 aaatttgagt cggaattgtc gacataattc catatggtaa gattaaatag aataatactt
    57841 taaaaacacc ttcatatatt acaccaggaa ttttcatatt cattggcaat aaattatctt
    57901 ctaatctcat tatcgcagaa gatgatataa caaaaaatga aactgttcta agtatgatac
    57961 atacatcata aaaaagtaat gtcatcaata acaccatgaa aatatatatt attatcataa
    58021 ctaaagttat ctctgagttt aactcagata ttccatataa aacaataagt atacttgcta
    58081 gaattaatgg tgatgaacca atattaatat tttcaaatgt taacctaaat agtgggtcta
    58141 atggttttgt aatatagtag tctaattctc cttcttggat ttttcgtgga atatcataaa
    58201 taccaaaaaa gaaaatcgtc atatttatcg catttattaa tgagaaagtt cctataaata
    58261 ttaacatctc tccttgttgc catcccccta ttgagtctac atgagaatat atactattaa
    58321 acattagaag ttgtaatata aataatgaac tatctacaaa aaatgctcca aaaaaatcga
    58381 atctaaatac catcatcttt gataatttca tttttaataa cataaatatt attttcaaat
    58441 tttttttcat catataccca ccccatcaaa taatgttctc attcttttat acataacttt
    58501 atttactaca gaaaaaaata aaacccataa tgctagaata attaatcctt gtattgcctc
    58561 attgctgttt ctacctatta gtaacataga tggcaaatat gttgtataat aaaatggaaa
    58621 taccttcata actgttacta tagtttctgg taagagtaca agtggtatca ttgttcctgt
    58681 aataaattca acaatatttt caattatcat tttaaaaaaa ctaatctcaa gaaatttaaa
    58741 tgacaaaatc ccaataaaga agtggagttt catcataaaa ataaacccta gaaaaatcat
    58801 caaaattgaa attaatattt gaactggatt tgatgtaaag gcaaaatcta ttttaaatat
    58861 aaaaatccat acaaatgcag aaatcatatt aaatacaaaa taaaaacatg tcattccaaa
    58921 atttttagca ataaaataat tttgaacatt aattggaata atcatgtact ttgaaaaggt
    58981 gcccaatttt acacgattac atatctcttc actggtactc tttgacatat caatacctgc
    59041 taaaaatgag cttattatat agtaagacaa catagagtta aatgtaaaac cagagactat
    59101 tttattttca ccaaaaattg ctttccataa aatataagca aataaaattt ttacaatcgt
    59161 aaacaccata ttaaccacca catcaaatct ccagataagt tgcactttaa agttaatttt
    59221 ggctacttca taataccttc taatactctg tttaaattta taaaacattg aatcatttga
    59281 acttgcttca ttatagttgc tcataatact catcacttcc tttcttatat attctctcta
    59341 caatactacc tatatcttct tcttcaatca ttacatcttt aacatcatat ttatcaaaaa
    59401 tccatttaat cgtttcattt cctctttctt ttggcacaat tactgatatc ttatgatttg
    59461 tttgttcaac aactttacaa tcttttggaa atacaatgtt agtttcattg gaaaaactaa
    59521 ctgttatttt tttatatttt tgaaaatgat taaatagttt ctctgtagaa ccatcatata
    59581 ttttacggct attttctaca acaatacttc tactacagag actcttaata tcttccatgt
    59641 aatgagaagt aagtataata gtagttcctt ttgtttcatt tacttctttt aggaaatacc
    59701 taatttgttt ttgggctata gcatctaagc ctatagttgg ttcatccaaa aataatattt
    59761 ttggattatg tagaagggct actattagtt ccattttcat tctttctcct aaagaaagcg
    59821 tcctaacttg aacatttaaa tagtcagata cctcaaataa ttcgataaag taatccaagt
    59881 tttttttata ttcttcatta gatatatcat ataattcttt aaataaaaga aacgtatctg
    59941 ctggtgtcaa ttcaaagaat agttgacttt tttggcccat aacaacagca tattgttttt
    60001 taaaattatt ttctaatttg ttagggtaat aacccattac agatatttct ccagaagacg
    60061 gtgcaataat accagttaac atcttaataa gagtagtttt accagctcca ttttgaccta
    60121 ttaatccaac aaattctcct tcttctatct ttaaatcaaa tgacaatata gctgtttttt
    60181 ctgtatattt cctattaaat aaagacttta cactccccat aaatcctgcc tttttctcaa
    60241 atcttctata agctttagtc agttcattaa gttcaattat actcatttta actacctcca
    60301 tatttcataa tttatataaa aaaagtccac aggtatatat ctgccttttt ctgtgcttat
    60361 atttaacagc acaaaaacag aaaactcctg tggactagaa aaattaatgc tctctatttc
    60421 aaaatagtta tcaaaataat actatacaaa attaataaaa gtgtagcaga ttaaagccta
    60481 ctacaccata atttaacaaa gtaatatata ttttaaatac tatttgttgt catatgtcag
    60541 ttatctataa aggcagaata agacacaaaa attaaatgta ttaaaaatac acttgaaatt
    60601 tctgattata cagaatatat gtgtctccaa ctatttaatt gttggttacc attatataac
    60661 tccgacatca aggcactcct ctcataatat tattattgat atcatatcat aaaggttatt
    60721 tatgttcaag catggattaa tatactaata catttagctt atttcttcaa ggttgaattt
    60781 aaatttcatt tataatattc gtttataaat taaaaatata atacgtatat taaaactggt
    60841 cattttactg taatattttt attgtaaaag atatactttt acctatacag gatgtaattt
    60901 ctttagttct atattataat aatattgtaa taagagttca acttataata cattaaaata
    60961 taaactgtaa atcttaaggg ggtaatctat ggaaaataat acaatacaaa aacaattaag
    61021 agatttatca aaaaaaattt ggcatattta tattttaaat ccagaagaag aaaaccttaa
    61081 atttattatt tccttattag atgacaacct tgcattaatt ggaacaggaa aacatgaatt
    61141 ttacgaaaca ttagaagaat ttattatagg tcttgaaaaa gaccaaacag aatcagagca
    61201 aatagacttt gagattatag atgagtggta ttcatgtacc aaagtcacag acgaagcttt
    61261 tatagtatat ggtactcttt gggtaataga aaaagattac ctagaaaaag atatctatgt
    61321 agaaatggat actcgattta ctatggtata cagagttaca gatgatatga ttaaacttat
    61381 ccatgttcac cattctattc caaactttga ccaaatgaaa gatgagtatt accctaaaac
    61441 aataactgaa aaagcacaag aagcactagc aatggcaaat gaatttaaac taaaatcaga
    61501 aaaagacctt atgactggac tatacaatag acattttttt gaagtacaca taaaagaaat
    61561 gcttaaaact gtttcacatg gatactttta catctttgat ttagataatt ttaaacaggt
    61621 aaatgatatc tttggtcatg ttcgaggaga tgaaatacta atacattttt caaatatttt
    61681 gactaaagta ttcgtaaatg atgctattat tggaagactt ggtggagatg aatttgctgt
    61741 ttttgaatat agtaatcaat caaaagagaa tgttgaaaat aaggcaaaga aggttttaga
    61801 attatgccta gagagtaatt cttgttctat aggtatatct aaattagaaa ataactctga
    61861 tactttccag tctttataca aacaatcgga ttatgctttg tattattcaa aacgaaataa
    61921 taaaggaaac tttagttggt tttagtacaa aagatattaa aataggcaaa tttattagag
    61981 tatcttgtcc tatatactaa taagtaaagt aaacaacaag ctatctttgc atatatttat
    62041 ataaaccaac tattcttaat aaaactacaa tagtcaacat gaccactatt atagaatata
    62101 ataaactaaa aacatgatta atattatatt ttaataattt tatttttaaa agtaagtcca
    62161 aaatgttata tgttttgatt aaaactgatg ttgatataaa aaattttact taaaaaaaca
    62221 cttagaaagg ctttataaag agcctttttt gcttttattg attaaatttt catataaaat
    62281 tccttattaa atttcaaatc aataaaaaaa ggctctataa gaagcctccc cctattttta
    62341 ttgattaaat cctaatatag acaatgcctt agcatcatag taagtagtca tctctcttgt
    62401 tgcatccttt aatttacggt aatcaaagtc taatgatcca ccaaaagctg atatttcaga
    62461 cccatcaaac ctataccatt tcccatcaat ttgtatatag ttccaagcat gatttaatga
    62521 tggatttaat atagatagtg aatccattcc cattaacctt gttaataaat gaaagctact
    62581 tgcaaaactt ccacatacac cacgtttatc aaccagatta ccatagacta tgttacaata
    62641 tttagagtct gcatattttg cttcggttac caattttgca gctctattag ctctttccat
    62701 ctcagatgca tttctccagt caaagctatt taaaaaattc ctgattactg taagtgtatc
    62761 ttgagcttgt ttgtaagaaa tccaatttcc atcagctatt acttctatag ggtcaagttt
    62821 tccaaagcct attctctgat atgagccacc tgacaatgct gccgcataaa atcctagccc
    62881 ttccataccc ttatttatat aataatattt accatctgct cgaagctcaa ctccattggg
    62941 atttcctcca tagagtaaat cccatccctc tttacctgtt gtattaagac caagttgtgt
    63001 taacattcct ttaagaggat atttagggtc ataattagca cctttactta ttgcatttgt
    63061 ttgcactctg ccatccattg tgcctatatt agtctgatta cgttcatttg cactctgttt
    63121 tttcaagcta gatgtttcat cttcttgata tggctctgcc attactgtca tactcatcat
    63181 caatataata atcatggtaa tcatagtagt taatatattc ttcttcatac tactccccct
    63241 tatttaattc tttttaaaac ataaaactat taactcataa atatatggtt attagtaaat
    63301 tgaaattaag tctggttcat aaattattgg tttaatagaa gtgtattgaa attgtacctt
    63361 tatattttca ttatataata aacttatttt tttaaaacat attgtttaaa tcagtacatt
    63421 tatatgtttt ttttgttcat ctgttttatc aaatatatta gtctttgaat tttgtttgta
    63481 ttcttcagca tttttatcta acttttctat tctcgactca agtttcatta tttcttcttg
    63541 tgttaaattt ataaaatttt taagcatatt ttcaagctct tcacgagttt tacctactaa
    63601 agataagtcc tcagatactt tctctttttt attagaatcc tctttataat cctttttttg
    63661 ttctaactct tctaattttt catcatttaa tatttcttct tcaatttttt cattagactc
    63721 tttagtgatt aaagattctt tttcatcatt tgtatttaga taagttgaat tttcatctaa
    63781 cattgtactt ttatttttct cttctataat gctattttta atacctattt ctatattaga
    63841 aaaattatta gttgtttctc ttatttctga tttacataag ttcttaattg cgtcatttat
    63901 ttttttagta taaacctctt gtctatattt taaatctcta attctatcat taagttcttg
    63961 cttttgtttc ataatattag ataaataagt gtcttttcct acagtttcat ttttcttaac
    64021 catactttca tttattcttg tattatttat attactttca tatctaaatg aatattgact
    64081 tctatattga ctagatattt tcatttattt cacctcctta aaataaattt atgtttataa
    64141 aaaccaactt aacttaatta tattttatca tcaaaagtat acatttacaa atattagtta
    64201 atagttttat aggttcgagc attattttca acaaaaaatt tttattataa attaaaacaa
    64261 taatttttat aaatatatta tttgtatttt ataattcata tatttttact ttataaatta
    64321 gcaatattgc agttttgctc ttaagaaaca tctctatcat acataagcat atactctatt
    64381 atgtattagt ataaatatgc cttttcttat cttacataag acctctttat aaaggagcct
    64441 atcgtaacaa atatgtatac attatcacat gtatatatca tcatataaaa aatcaaactt
    64501 tattaaaaaa attaataaat tgctttaaat aataccacaa accaaatttg ccatcaaaat
    64561 cacattaaag taacaaattg atggcaaatt taatatatta atagtatata gactcataga
    64621 atgaacaaaa aaactacact tatcttctaa tcttcttatt tatttttgag atatttttaa
    64681 atttcttttc tttttttgct aagtattcgc ttttattgtc tgcaaatact gcttcagttt
    64741 taagcctacg ataggattcc catcttttag gcgaaagttc ttgatgagca atagcttccc
    64801 agatagcaca gcctggttct gaggtgtgag tacaattacg aaaatgacat tttccgaaat
    64861 aagcttctat atctgcaaaa cttttatcta aaccttctga aacatcccac attcccaact
    64921 ctctcatacc tggtgtgtca ataacaatac caccacctga aattaaaaat agctctcgtc
    64981 tagtcgtggc atgtcttcct ttatcatcat ctatgcgaat ttcctttgta tttaaaaatt
    65041 ctccacccat cagacaatta attaatgaag attttccaac tcctgaagaa ccaagtaagg
    65101 caacagtaca gcctggttta atatacttag aaatctgttt ataaccatct ggctctatag
    65161 atgaggtaac aactacatct acacccaagg ctatagaatt aactacttgt aatttacgat
    65221 gcacattgtc acataagtct gatttagtaa gtactactac tgggatagct ccactttccc
    65281 atgttaaaga cagatacctc tccaatctat gaatgttaaa atccttattc agtgacatgc
    65341 acaaaaaaac aatgtcaata ttagctgcaa ccacctgctc ctcatttacc tttccagcag
    65401 ccttacgcac aaacaagctt tttctatgaa gcacatgatg aataatagca ttaccatttt
    65461 tattattgca acgatccaac ataacgaaat caccaactgt gggaaaatca gattgtatag
    65521 tagcctcaaa acgcagcttt cctgaaacct ctgcaaacag tacaccattt acacatacag
    65581 cttgataaaa gttcttttcc tgcgagataa tccgagcaag gtataaatca tgatattcca
    65641 tactttgagt aataaaattt ctatttagac catagtcttg taaattgatt tgtttcatac
    65701 cattcattct gtttcctcct tttccaactt ttcaatgatt ctttctagtg ggaaaggggg
    65761 ctgtgccagt ggttttaatg caattgacat taatttcact gaattatcat cagctgcaat
    65821 acgattagaa ctaagatgtc cacagcgtgt acaacgatga ataattgccc actcaccatt
    65881 ttttctcacc cacactccaa taggttccat attagcatca caatctgcag cacggtcacc
    65941 aggaatgata tctacatgaa gactatgtaa acaatgtgga caatgatttc tatgttgtgt
    66001 accagctcct tcaggaacta caaggcaacc acaatttttg caaataaagt tatccataca
    66061 ggaattttcc tgataataag ctcgttttct atttttataa ttcattttta tttcctccgt
    66121 atcgtgtatt ttctacaaaa aaagtccaca ggtatttata tctgcctttt tatgtggtat
    66181 acatatttac ctacacaaaa acagaaaacc cctgtggact agaaaattta gtatcttcta
    66241 tttcaacata attatattca aaataatact gtgtaaaatt aataaaagtg tagcagatta
    66301 aagcccacta caccacaatt taacaaagta atatgtaata ttattatttg ttgtctaatg
    66361 ttagttatct ataaaggcag agtaagacac aacaatcaaa tgtatcaaaa atacacttaa
    66421 aaattctgat taaacagaac ccatatgcgt ctccaactat ttatttgttg gttaccatta
    66481 tatataactc tgacatcagg caagacactc cttcactaat aattattatt gaaattttat
    66541 cataaaatat aagtatgttc aagtataatt taatatattc atatatttat gtttgtctca
    66601 ttttcttatt ctataaaatc tagtggtatt ttgaaattta atgttcacct agtattaata
    66661 ataaaaatta actgctatta tttgttttct cattttatgc ctgttgctca atttatctca
    66721 cttaaattaa taacaactgt cttttcatac aaaacctaca atcaagtttt tccatatatt
    66781 ctacatgcat agtctatttt ttttgatata aaataacgtt catatttgta aatttattta
    66841 attttcaaat aagtatattg aataataagc aattctattg tattattgta attgtagaat
    66901 aaaactaaat cggcaaaact agagaaattt agtgacgcaa agctataggg actaacgctt
    66961 ataaaataaa ctatgagtta tgtcagccag ttgccaaaaa gagatattct ttttgttttt
    67021 atgaaagttt tttaggggga taataatata tatgtttaga gataaaatgg atagatgtac
    67081 acatatgttg actgcttata ttggtagttc atatgattat tgtgatttta tagatacaca
    67141 actagatgat tttatactag agtacggaga aaatgtggta gaatcttgtt tgcatcaagt
    67201 gatggtatta gtaagtaagt ataattaaat agatttggtt ttctagagaa ccctttttag
    67261 ttttatctac tttaaatagc cttatgttag aaagctaatt aagatagatt tctagtattc
    67321 ataattttat tttttattta aaatacatat aataaagatg taaatttgta tcaaataata
    67381 aatatgagaa cttaaattct cttatctatg tgaaaataaa catctcttaa aatgaaattc
    67441 tgtaaatata aatgattttt tagtttgaag gattagatta acaaaattat aaatttgcaa
    67501 ataagctata ttaaaaaata taataatttt ttatataact tattctttta taacttttaa
    67561 ttttaaaaga ggagaatgta tataagagta ttcaatgtac tagaaaaaat caaaagaaat
    67621 attagaagaa atggatattg agtaatcaaa gaaaagattt tgatattaca aatgatatgt
    67681 ttcaagaaag cattcctatc acaacaaaaa ttattcttga agacatgcct tcaaatgatg
    67741 aattaaatca tgttttttca aaaggttgtg aacgaaaaat gaaaaagaga aaaattgtat
    67801 taatcacact actattaata ggtgttttgt tattaggaag tatcctatat aatctttttt
    67861 taggaaaaac agctaatatt tcaatgctaa aggaatcttg gaattttgat ataccaatac
    67921 ctaacaaaga aattgaagta tttgatactc aagatagtat taatggagat ggacaaagtt
    67981 attttattca aggattttct gaaaaaaatt tcaaaaaagt ttttaattta aaaggtggta
    68041 tagttgtaag caaagataat attaatgaaa tagaaaaata tattgataaa tttaaaagag
    68101 attctgttaa cattaataaa agtaataaaa ataaaattga agaagatttt aaaaaatata
    68161 agttagaagt taaaaaagat gataagtata tatataagag aaattatgag aactacgtag
    68221 ttttaattat aaaaaaagat gaacaaaaat tatacagttt aatatggaat cagtaggtac
    68281 atatgtatta ttaagaatta taaataataa aaataaattt tagtcaatat acaaaaacct
    68341 tttaaattgt ctggaaatca cctcttcatt tgttataaaa acatacaaca aatgaagagg
    68401 tgattttatg gttttaaaaa aaataattat aagtggatat gtgctttcac atgtttatca
    68461 acatttgtaa tgcctatatt tgctaatgaa ttataaaaat attaaataat attcaactat
    68521 agattatgta aattactcta tgaaaaaaat gagttgtata aaatctggct actgtagatt
    68581 gactatttct tttaaaatta caattataat aagttttata gaattaaaaa taaaacttta
    68641 gagatttttg ataacattgt agactaaatt tgccaccgaa atcgctttac agtggcaaat
    68701 cggtggcaaa tacaatatat tattagtata taatctatat aaacattact attgtttgat
    68761 tatcttagct acagtttcca catgtgtaga atgagggaac atatctacag gttgtgcctc
    68821 aacacattta aatccgtgaa catttagata atctaaatcc cttgctaaag tagaaggatt
    68881 acaagaaaca tagacaattc tttccggatt cattgaaaca atagtctcta ataccttttc
    68941 gtcacatccc tttctaggtg ggtctacaac gactacattg gcagtcttac cctctctata
    69001 catcttaggt acaatttcct ctgcctttcc aacataaaat tcagcattat gtatattgtt
    69061 taatttagca tttatcttag catcttttat ggcatcatct ataatttcta ttccataaac
    69121 ctttttagcc ttttgtgcca aaaataaaga tattgtacct atcccacagt aaatatcaaa
    69181 aacagtatca gtatcattta aatttgcata ttcaagagcc ttatcataca atactttagt
    69241 ttggacaggg tttacttgga aaaatgataa tggtgaaatc tcaaacacta agtctcctat
    69301 ataatcgttt atttttccat ctccatatat aactatattt tctcttccta gaatagcatt
    69361 cgtatctttt tgatttacat ttacaaccaa agtcttaaat ccaggtatat tttccttaag
    69421 cactgaagct aattctttaa gatatggaag tttgtttcca tttgctacta atacaaccat
    69481 tacttcttta gttgtaaaac ctatttttgt tactaaatgc ctaatcaacc ccgtgtgagt
    69541 cttttcatca tatatgctta ccttataggc ccttatatat gtttttatta ttttaacaat
    69601 tttatcattt atctcatgtt gtattacgca tttttctgtc gatattacat catgagtctt
    69661 ctttttatag aagccaataa ttggaatacc attattcttt tgtattggaa attgtgcttt
    69721 atttctatat ctatatggat tatccattcc tatagtgtca tgaacttcta catcctctaa
    69781 cttacctatt ctcgaaagta cttgtttaac ttcatttgtt ttcatttcaa gttgtttatt
    69841 ataatctaag tcttgtatct gacaccctcc acaatcccta agattgtcac tacacactct
    69901 tttcaccctg aatggagact tttctattat ctcaacaata tctcccacag cataattttt
    69961 ctttacttta ttaatcctaa ctttaacttt atcatgtatt aatccgccct ctacaaaaac
    70021 tgtaatacca ttgtactttc ctactcctac tccgccttga ccaatgtcta ctatatctac
    70081 aatatacatc tcgtttttcg aaagcataaa tcaccctcca atcaatctat tatatttaaa
    70141 tctttacaaa cttttttctt taaatttata ttaaaatatt gctattaatt aaactatatt
    70201 ttttactaag ctacaatatt ttattttacc aaaaaaaaag gagcttataa agccccttag
    70261 taaataaatt tattattttt cttataaaac tctagttgaa cctctatata ccacgcctct
    70321 tgatgcatcc acagttacaa cttctccatc ttttactaga tttgttatat cagctgcaga
    70381 cacaataact ggtatatcta aatttatacc tacaatagct gtatgtgatg tcattccacc
    70441 attctcagtt actacagctc ctgctttttc tatataattg ttcatttcag catcagtagc
    70501 agtagaaaca agtatgtcgc cttcattaaa gtctacacaa gcatttccat tctttattat
    70561 acgaacttta ccttctactg tttgagtacc tacacctata ccttgcacga tttcttcgct
    70621 tattacatga acctttatta aattagtagt tccacttact cctactggta ctccagcagt
    70681 tataactact aattccccat tatctatata atcagcttgt ctagctgcct caattgcctt
    70741 ttctataact tcatctgtat ttcctgcaac agaagactta actggatata ctccccatgt
    70801 aagagctaat ttattcatag tcttttcatt atttgtagta gctattattg gagattttgg
    70861 tctgaattta gataccattc tagcagtata tcctgaagaa gtagatgtta ttatagctga
    70921 tgcatttaaa tctactgctg ttgtacaagt tgcatggctt attgcatctg ttacagtaac
    70981 attgttagta ccattttcct ttagtaatct atcataatct aaagtctctt ctgttctttt
    71041 tgctattgta gccatcattt ttactgcctc tacaggatat tttccagcag cagtttctcc
    71101 agataacatt atcgcatctg ttccatcata tatagcattg gcaacgtctg taacttctgc
    71161 tcttgttggt cttggatttc ttatcataga atcaagcatt tgagtggcag taacaactgg
    71221 ctttgctaat tcattacatt tttttatcat cattttttga actattggca tctcttcagt
    71281 tggtatctca acacctaagt cacctctagc caccattata ccatcagaaa cttttaatat
    71341 ttcatctagg ttctctacac cttcttggtt ttctatttta gatattattt gtatatcagt
    71401 tgcattattg ttctctaata cttctcttat tgctagtaca tcagatgcct ttcttacaaa
    71461 agaagcagct atataatcta ttccttgact tattccaaac tctatatcac ttatatcttt
    71521 aggagttatt gctggtaaat ttatcttaac accaggtaca tttactcctt tatgattttt
    71581 aactattcct gaattttcaa caacacaaac tatatcttct ccatttattt cttttactct
    71641 taagccaact aaaccatcat ctattagtat agaatctcct gatttaacgt cttcagcaag
    71701 acctttatag cttactgtac acatttcttt agtccccata atatctttca tagttatagt
    71761 gaacttttgc ccttcttcta aaaatacctc tgggtcttca aagttacctg ttctaatttc
    71821 tggaccttta gtatctaata atatagcaac tggcttattt aatttttctc taactttctt
    71881 tgccatatca attctttctt tatgttcttc atgcgaaccg tgagaaaagt taaatctaca
    71941 cacatttaga ccattttgca taagttgagt taaaacttcc tcactttgtg atgctggacc
    72001 tagtgtacaa acaatttttg tcttctttac attatttaac atatttgctc ctcctgagat
    72061 attattccac agatttattt ttaatactaa aacttatata gataatattt ttgccatctc
    72121 ataagcttct ttatcaaata ctttcttttg agctaaagct tcacttattt ctaaatcaat
    72181 tattttgttt tcttttattc ctacaactct agctgatttt ccatctaata ataattctac
    72241 tgctcttact ccaagtctac ttgctagtat tctatcagat acagttgggc ttcctcctct
    72301 ttggacatgc cccaatacag tgactcttaa atctgctcct gactcttttt taagttcctt
    72361 ttctaaatca ctagcacttc cgactccttc tgctaaaacg attatactat gtctcttacc
    72421 tcttttttga gtagtcttta atcttagagc aacttcatct actgtaattt ctacttctgg
    72481 tactattata gtttccgctc ctcctgcaag acctgcatat aaggctaaat ctccacagtg
    72541 tcttcccata acttctacta tgttaactct ttcatgagat gatgatgtat ctcttatctt
    72601 acctatagca tctataatag tattcattgc agtatcaaat cctatagtat aatctgtata
    72661 tgctaaatcg ttatctatag ttcctggtat gcctatagct gggaatccta agtcactaag
    72721 tttttgggct cctgcaaatg aaccatctcc accaattaca actaagcaat caattttata
    72781 ctttttaaga atttttactg cttttaatct accttcttct gttttaaatt cttcacatct
    72841 ggaagatttt aatatagttc ctcccctgtg tataatatct ccaacagaag ataagttcat
    72901 ttcagttaaa tcttcttcta gtaatccctt atatcctcta ttgattccat atactttgca
    72961 tccataatat attgctgatc ttacaaccgc tctaatcgca gcattcattc ctggagcatc
    73021 tcctccactt gttagtaatc ctatagtttt cattttttac ctccttggat tttatgtcaa
    73081 ttttttatta taatcattgt tattgtattc taatggactt tttttgtccc tatatcactt
    73141 tttaatccca tcaagactaa aaaaattaaa tgtccttaaa tgtccatttt gtgatattat
    73201 atcacaaaac ggacaatatt tttcatatta ttataatata tttagatttt tatataaaaa
    73261 atcaagcttt tacttttatg ttatctttag gaagtaactc ttctaagtta tttataaatt
    73321 catcacatat acaagtcaat gccccggtca ttttatatgc tttacgttta ccatttatat
    73381 tttcatccat agggtataag tatatactgt cattacctgg atattgtttg gctatttcta
    73441 caatattatt agttgtatca gtatcagaca tactatctat tttcaaatac aattttttac
    73501 cactttctgg cttaatattt tctacatttc tagttcttcc tgaattgtag tttctatctg
    73561 caaagtcagt atcatcattt atatctttta tctctctagc tataagtttt gcattttcgc
    73621 cttcttttat acttaatgtt ccttttacat atattatatt atcttcattc aaaatgacat
    73681 tatacttttg taataattga ggaaaaacta ttatttctat agcaccatac aaatcttcta
    73741 attcaataaa tgccataatt tcatttcgtt ttgttgtttt aatagtttta gttacaatca
    73801 ttccacccat tataacatct gtttcattca tttgtaggta tgactcttct tcctctttta
    73861 atgaatttaa ttttccatta tccatagatg ttttttcttg tagttctttt tcaaattgtg
    73921 ataaaggatg tccacttaca tacattccca agacttcttt ttcaaggtta agtctctctc
    73981 tttcttcgta ctcttgcctt ttaggcaaac tgtatatttg ttgaaaatca ctatcttcct
    74041 cactatctcc aaaagcatca aataaagaaa tttgaccttg aacatttttc tttctatcca
    74101 tagatacact ttctaataaa ctttcataac cagccatcag actagctcta ttttcactta
    74161 tattatcaaa agctccacat tttattagac tttctattac tctcttattt gtatcttttt
    74221 ggtctaatct tttgactaaa tctactaaat ctacaaataa tccattagat tctctttcag
    74281 cgattatatt gtttattata tttacgccaa cattttttac ggctgctaga ccaaatctta
    74341 tattgtcgcc ttcaactgaa aactttgaaa aacttttgtt tatatctgga ggtaatacat
    74401 ctatttctag tgctttacat tctcttatat actcaactac cttatcagta tttcccatta
    74461 cactggttat caatgctgcc ataaattcaa ctggataata tgtttttaga taagctgtct
    74521 catatgctaa aaccccatat gcagctgcat gtgatttatt aaatgcatat ctagcgaagt
    74581 ctatcatatc atcaaatatt ttattagcta tttcctcttc tacaccattt ctgatacacc
    74641 ctgcaatttc tatattacca tcttcatcaa atttaccatg aataaaatat tgtctttctt
    74701 cttccatcac gtccatcttt ttcttactca tggctcttct aactaggtca cttcgtccgt
    74761 aactataacc acctaaatct ctaacgacct gcataacctg ctcttgatat accaaacaac
    74821 catatgtaac ttccattata ggctttaatt tttcatgtag atatgttaca tcttttgggt
    74881 tgcttttatt ttttatatat gcaggtatag aatccattgg acctggtcta aatagggata
    74941 ttcccgctac aatatcttca aaattgtctg gttttagctg tttcataaaa cttctcatgc
    75001 cagcactctc caattgaaac acccctagtg tatttcctga tgaaagcatt tcatatactt
    75061 tcgggtcgtc ataatccatc tttgaaaagt ctattttttc tgtgtaattt tttataactc
    75121 tatttttttc tattaaatca agagtatctc ttataactgt aagagttctt agcccaagaa
    75181 aatccatctt taaaagccca agttcttcaa gtgttgtcat agtaaactgt gttgtgattg
    75241 catcttgatg tttgtacaaa ggtacatatt catctacggg attttttgat ataacaactc
    75301 ctgctgcatg agtagaagca tgtcttagca ttccctctat ctgttttgat atatttatta
    75361 tttcttttgt ttccgggtct tggtcataca attctttaag attaggattt gtatctaggg
    75421 ccttatctat tgtcattcct aaagcaaatg gtatttcctt ggctaccttg tcaactttat
    75481 tgtagcctat atccaatacc cttccaacat ctcttattgc agcttttgca cccattgttc
    75541 caaaagttat tatctgggct acatggtcat caccatattt tctctttaca taatctataa
    75601 cttcttctct tctctcataa caaaaatcta tatctatatc tggcatggat atacgttctg
    75661 gatttaaaaa acgctcaaat aatagagagt actttattgg gtctatatca gttattttaa
    75721 gtgtatatgc tactatcgaa ccagccgcac tacctctacc tggtcctact ataatattat
    75781 tttctttaga aaaatttata aaatcccaaa caattaaaaa atactctaca taacccattt
    75841 tttcaattac atctaattca taatttaatc tctctagtat ctcattacta ggattatcat
    75901 atctctcttt taatccatta aaacacaact cccttaaata agtttctggt gtatatcctt
    75961 ctggaacatc atacttagga agatgtatag tattaaaatc aaactccaca ttacacatat
    76021 cagctatttt cacagtattg tcaatagcct ctaatgcata tggaaataat tcttccattt
    76081 cttctcttga ttttaaataa aactcatcac taccaaatct cattctattt gggtcattta
    76141 atgtttttcc catttgaata cacataagta catcatgtat tttcgaatct tctttattta
    76201 catagtgaac atcattagta gcaactagtg gtataccagt ctcttttgat aattttacta
    76261 aaccagagtt tacttctttt tgctcaggta aattgtgatc ttgaatctcc aaaaagaaat
    76321 tttcttctcc aaatatgtct ctgtagtcaa gagcaaattg ttttgctttt tcataatttc
    76381 tattcattaa agcacgtgct acatctcctg caaggcatgc agaaagtgct ataatccctt
    76441 tgttatgttt tttaagttct tccatgtcaa ctctaggctt atagtaaaaa ccttctacgt
    76501 atgaagttga aactattttt attaaatttt tatatccttc catattctta gctaacagaa
    76561 ctaaatgtcc ttgatatttg tcatagtttg ggtctttatc tctcaatcca cgtgcagctg
    76621 tatagacttc acaccctata attggcttta ttccttcttt tatagctgtt ttgtagaaat
    76681 ctataacacc aaacatacaa ccatggtcag ttatagctat tgagctcatg tttagttcct
    76741 tagccctttt tataagtttt ttcaccctcg aaaacccatc aagtaaacta tactcagtat
    76801 gtacatgaag atgagcaaaa tctttgttca ttaaatcacc tcctgcttta taattttaat
    76861 tttaccacaa atatatgtcc taaaacaaaa acagagactt ttatctcaaa ataggaatta
    76921 acttcaacgt ataccattat atgttataaa ttaatgctta ttctgagatt gtcccctcaa
    76981 tttattgcta tgattttttt ttcaaatctg ttgcaatttc ttctattttt ctaagtctat
    77041 gattgactcc agattttcct attggtggtt ctaacatctc tccaagttct ttgagagtca
    77101 tatcctcata ctcgattcta atcttagcta tctcttgtaa attttctggc aaactgctaa
    77161 ttcctatagt ctcttgtatg agcttaatat tttctacttg tcttactgct gcatttactg
    77221 ttttagaaag gttagctgtc tcacaattta ctatcctgtt tacattattt ctcatttctt
    77281 ttactatttt agtattttga agacttaaaa gagcgtggtg tcctcctata atactcaaca
    77341 aatctgatat ttgttcactc tctttgatat aaacaacgtg attatttttt ctagccacaa
    77401 ttttactatt aaaacctaaa gagtttataa gttcttttag agaatctgca aactcctcat
    77461 tattagttac aaattccata tgatagttct tttctgggtc gcttatagaa cctccaccaa
    77521 gaaaagctcc tcttatgaaa gctctcttac atccttcatg cttaattaag ttctctgaaa
    77581 ttttgtttat agtaaaaaat ccttctttat cttcaagtat acctaacttt tttaataaca
    77641 tttctgaacc catttcgctt gttaccatta atacataact gttatttctc tttagcattt
    77701 gattcttatt tactgagatt gtagtattga tattaaaatt cttttttaat aatttaaata
    77761 ctttacgggc tattgaattt aactctgttg ttatctttag atttagcttc ttatatccaa
    77821 caatttgtat acttccccct gatttaacta gagctgacaa ttcagctata ttacaacatt
    77881 cattcccaga tataactctt gctagttcat ttttggtttc agttgaaaaa gacatatcta
    77941 tcacctatta actaattttt ctttaacctt ttttttattt cttgtaaggc aacctttatt
    78001 atctcctttt catcatcata cctagctaag tctactactc tatctaggtg gataaattta
    78061 gcatcaataa agccttcctc tttttgtgga atagtatcca tattctttgc ttgcatcaaa
    78121 taccaaaaaa ctgttttatg aactgctcta ttttcatccc agttttcttt gaatgtatag
    78181 tgtatttcac ctaaatattt caatatatct gcttttactc cagtttcttc acttacttca
    78241 cgtaatgctg cctcttggtt attttcccct tcttctactt ttcccttggg taacacccaa
    78301 tctccattga acttccttag taaaagaatc gtattgccaa acaggactac accaccagca
    78361 ctaacctctt ctctcatcga tgttcactcc ttaaaactaa cataatgata aggatttcta
    78421 cacaattttt taaaatcctt ttttactaac ttttgttgta attatgattt agggctaact
    78481 caataactat gttagatata tactttgcat catgtcttat ataattattt tttatttcaa
    78541 ttaaattctt ctctacagtt tttataccca tctcttttaa tttatctttt tgctttttat
    78601 ctaacaagac ttgctctgct ccatcttgtt tatataagtc aagcatttct tctggcaata
    78661 tctcattatt tgcgattaca taatctatta aattctcatc agtgtgtttt actatagcat
    78721 taacatgctc taatacattg tacccttctg tctcacctgg ttgagtcatt atatttgcta
    78781 tatatacttt tggagctaca gacgatttaa tagcgtctac aatcccctct acaagtaaat
    78841 ttggtattat acttgtatat aaacttccag gacccataat tataatgtca gcatcataaa
    78901 tagatgcaat aacatccttt aatggtttta catcttttgg atttaaaaaa attttatcaa
    78961 tagagctttt ttgaattttt gactcttcag gtatactaga ttctccattt attatattgc
    79021 cattttctaa tttggcaact aaatttacat cttccaaagt tactggtaaa actcttcctg
    79081 ttatagcaaa aatttcactt aatttatata cagctttttc aaagtttcca tacaacccat
    79141 tcatagcagc caaaaacaaa tttccaaaac tctgaccttt taaaagccct tctgtaaacc
    79201 tatattgcat aacttcattc attgttggct ctatatttgc aagtgctaat aaacagtttc
    79261 ttatgtctcc tggtgggagc attcctaagt cttctctcaa tacacctgaa cctccaccat
    79321 catcagcaac agttacaatt gcagttatat tttgcgtagt gtgtttaaga cctcttaaaa
    79381 ataccgattg accagtacct ccacctatca caaccacatt tggttctcta ttttcaacaa
    79441 tagacctttg aaatagtttt actttttcat tttttgcttt tttatatata aataacgata
    79501 caattaatgc aatccaacct aaaattccta caataataag taatttagcc atgtttgcct
    79561 ccattatcgc aacatagagt ctctatgttt ctttacaact ctatatccct tattagataa
    79621 atcctccgct ataagatttg ttatagtaac tgacctatgt cttccacctg tgcatccaac
    79681 accaattact agatgttgtt ttccttcctc tatatattga ggtactaaaa aatgaatcat
    79741 atctaaaagt ttcacataaa attcttcact gattttagaa ttcataacat aatctctaac
    79801 ttctttatca tcaccagttt tagctcttaa ctcttcaaca taatagggat ttggaagaaa
    79861 tcttacatca aatactaaat ctgcatcagc aagtatacca tgtttaaatc caaaagatac
    79921 tacagaaata gttaggtttg gattatcttc accagaagaa tatattttct ttatttcttc
    79981 cttaaggtct ttaggtttca tgttagttgt atcaattata catgtagata actcctttaa
    80041 aggttccatt atctttcttt ccatttttat accttctgga atctgcatat cttttgccaa
    80101 tggatgattt cttcttgtca ttttatatct ctttagcaat acatcatcat tacaatccaa
    80161 atataccatt tcatattcat aattttcttt ttctaaataa tttaagcttt catgaagcgc
    80221 ttcaaaaaac tttctccctc ttatatctat ccctagagca actttatcaa tacttgaatt
    80281 tggttgataa cataattctg caaatttagt tatcaaagca ggaggtagat tatctacaca
    80341 ataaaatccc atatcctcta aagctctcat tgtctcactt tttccagatc ctgaaagacc
    80401 tgtaactata acaaatttca ttgctaactc ctccctttta tcttatgttt attatattat
    80461 aatgttttct actaacgata tatccagatt agagtcagat atagtcttca tagctagttc
    80521 aaaatcacca actctttctg gacaatgtgc atctgagcct attataaatt tattttctag
    80581 cccttttatt ttatttaatt gatttacatt caaaaaagaa tggctactat ttatctcaag
    80641 tctagtattt gtcttttttg ctgtctttgc tacttcttct atatacacat cgcctttatc
    80701 tcctggatga gtaattataa aaatatcatt attacttatc gcatttatta cagcttttgt
    80761 attatattct tttgcttttt cagttttaaa aatatagtta ttacaatgat ttaataagct
    80821 tcttaatgat gttggagtag aaccaaaatg atatccagcc ataatataat caaaatctct
    80881 aataaattta tcatccatat ctatatttcc tttatcatct aatatgttac actccatacc
    80941 taaaagtata tttatatcag tgtacttatc atttaaataa tctacttctt ctctcatttt
    81001 gagaatatct tttttcttta caccatatgc aatatgttta taactatggt cagatattcc
    81061 tattgttttt attcctttag aaatggctac ctttacatta tcctcaatag tccctttgcc
    81121 atgactatat aatgtatgtg tatgataatc agccagtatt ttcatttgtt aatcctctcc
    81181 aagtattttg acttcttctt ccaacataac cccaaacttg gcatatacag cactttttat
    81241 aacatatatt aaatccaaaa tatctttagc acttgcttct ccttgattga ctacaaatcc
    81301 acaatgcttc tcagatactt gtgctcctct taaagtaaga cctctcaatc cagtttcttc
    81361 tattaattta cctgcaaaat atcctgttgg tctcttaaat gtacttcctg cactagcaaa
    81421 atttaatggc tgtttagtta ttcttctcgt cgctaaatca tccattaaac tttttatttc
    81481 atcataattt ccttcttgta attctaattc tgctgataaa gctatatatc cgtttttcga
    81541 tattatactt tttctatacc caaattccat ctgttcattt gataattcaa atatattgcc
    81601 ttccatatcc atcagtctta ctgacttcac tatatccttc atttcaccac catatgctcc
    81661 agcattcatg gctaatgctc ctcccaatgt gccaggtatt cctgatgcaa actcaaaccc
    81721 ttttaattct tgcttttgga gagctttccc taaaactgat aataaagctc ctgactgagc
    81781 agtcatccta gtatcattta tctcataaga attgaaatta tcagctaatt ctattaccac
    81841 tcctcttatt ccaccatctt taattaaaat gtttgaacca tttccaatta ttaaatacgg
    81901 aatagactct ttttttacca acttaagtac attttttaat tgttcttcag ttctaggtct
    81961 aactaatata tctgctggtc ctcctaccct aaatgaaata tgttttttca taggttcatc
    82021 gacttttata tcctctttac ttaatatatt taataaattt atatatatat cttgattatt
    82081 cattgtattt cctcccaaat tatactgaac tttagctgtc attattattt aaattacatt
    82141 aatatatagt attaactata ttcaataata tatgattttg cttatcatat ggattttata
    82201 ttttaagctt cttaattaca aactatattc aataaatata taataaagta aaaaaaaaaa
    82261 aataacaaca tataaaagaa tataaataat ttaaaaaaag catcaatatc actgatatca
    82321 atgctttaaa aggcaattta attcgtatta tttatctaac tatttttgac atagcaaaga
    82381 taataaatcc ctccaactac tatggaacct ccaacgatat ttccaagtgt tacaaatacc
    82441 aaattagtta taaattgaac catagttata tttgctccta atgccattcc cattggtata
    82501 aaaaacatgt ttgcaacact atgttggaat ccacataata caaatagcat tattactgtc
    82561 caacatgaag ccaattttga cattatatct tgagatgctg tagccatcca tacagctaaa
    82621 acgactagaa tattacaaag tattgctttt aaaaacattg atgctatagt tgaattaacc
    82681 ttagcttctg ccacagctat agcttttgta gctgcatctc ctgtcaatgt agaagaataa
    82741 aacacaagtg caactagcaa aattgaacct actaaatttg caaaataaac cagtccccag
    82801 tttcttaaca tttttcctaa tgttattttt ttgtccataa gtgcaatagc cattaaagtg
    82861 tttccagtga ataattctgc tcctgccact actacaagca taagacctac tggaaagacc
    82921 atagcactta aaaagctagc tacccctggg tctatcttgc caaaagtttg actaactatc
    82981 atactcccca atgccccaaa acctataaat gctcctccaa ataaacctaa tagaattgca
    83041 ttttgtgtcg aaatatttgc ttttttaatg cctgcactta tagttgcttg tgcagtttcc
    83101 ccaggtgtca agaatccttt actcataaat ccttccccct taaaaattat actttccctt
    83161 gtctttttat ggcaataatt atataataac aatatgatat ataataaatt ttattaacat
    83221 aaaaaatata ctatatactt atatatactt gtctcatata attatactat ttattatata
    83281 acaactttgt taatttcgaa ataatttata ttgaatttga aaaatttacc ttttgattat
    83341 ttattttact ttttaattat tgaggtatta tatataatag atatatgttt gtgttattat
    83401 atataattac ttattttaat aaatatttga aggagggaat tttatgtttg atatatcatt
    83461 tttcgaaatg tcaatatatg aaatagcagt tactacaata tatataatta atttcatggt
    83521 tatactaaac cttatcttta gagaaaaaag aaacataaat acaactttaa catggcttct
    83581 tatattagta ttgattcctg ctcttggatt catactttat atagcttttg gcagaaatat
    83641 ttcaaaaaac aatatgttta gattgaaaga aaaggatgat aagataatca aaagtaatat
    83701 actagatact caggttaaac tccaatctac atctgaaatt gattcagata tacatcaaca
    83761 taaggatatg atttatgcat tggctaattc aaataatgct cattatacca ataataacga
    83821 tgtgtggatt tatgctgaat ctagtcaatt ttttaacagt cttcttgaag aacttaaaaa
    83881 agctaaaaaa tatataaaca tacaatttta cattttcaaa gatgacaaaa tcggaactga
    83941 aataatagac attcttatag acaaagctaa agaaggtgtt gaagtaagac ttttatttga
    84001 tgcagttggt ggtagaacac taaaaaatag tacactttcc agacttaaag aaagtggtgt
    84061 taaagttgga agcttcttcc catcattctt gaaaattgta aactttaact taaactatag
    84121 aaatcaccga aaaattgttg tcattgatgg taaggtagga tttgtaggtg gatataatgt
    84181 tggtgatgaa tatttaggta gaaatccaaa atttggtcta tggagagata cacatactaa
    84241 gcttactggt gattgcgtaa ttgacctaaa tatgagattc atccttgatt ggagatatac
    84301 aactaaagaa gacttagatt tagaaaaata ctttatagaa gaagatacaa attctaatca
    84361 tccttctgaa aatattggga ttcagatagt atcaagtggt ccagatatat cagaacttga
    84421 tgagattaaa tatggttacc ttaaaatgat tcagaaagct agaaagtata tatacataca
    84481 gagtccttat ttaatacttg actcaacttt tatagatact ttaaaaatag cctgtttatc
    84541 tggtgttgat gtgagagtga tgattccttc taaacctgac catccctttg tatactgggc
    84601 atcatattca tatgctggag aacttttaaa gtttggagct aaaatctata cttatggtca
    84661 aaatgcattt ttacatgcta agacaatagt tatagatgac tctatatgct ctataggaac
    84721 agctaatatg gatatcagaa gttttgagct taatttcgaa gttaatgcat ttatgtactc
    84781 ttcaaaaaaa gcattggaac aaagaattat atttgaaaat gatattttaa attctaagga
    84841 aataacatta gatatatata attctaggtc tacttatata aaaatcaagg aatcaatttc
    84901 aagactatta tcagcagttt tataaatctt ttattttata aaaattttac attttggttt
    84961 attttttaaa gttttttata tgtattattt tttttttatt gaatttagct catttttttt
    85021 atttttttta actattctga attattttat attataatag actagtatag tttttatttt
    85081 taataggtaa cttttttgca ttgtttttat aaataaagaa attgttgtat attattcttt
    85141 ctttattgtt ttggttttgt tcacccacat aatgttgggg tcaactcatg gttaattatt
    85201 ttatgaaaaa taataacttt aaaaagaaaa gggggatttg ttatgtgcga ttttatttca
    85261 cacaacaagc aattatttga cgaactagat atatttattg actctcttgc aacaaaggaa
    85321 ggtgcgttaa tacaagttct acatgaagcc caaggtatat ttggatatct tccaaaagaa
    85381 gttcaacttc atgttgctag aaaacttgga gtagctcctg caaaggtata tggggttgtt
    85441 acattctatt cttattttac tacagaacct gtaggtaagt ataaaatcag tgtctgtcta
    85501 ggaactgtat gttttgtaaa aggtgctgac aaaatcttaa gtgcttttga aaaacagctt
    85561 ggcataaaag ttggagaaac tactagtgat tttaaatttt ctttagaagg tttaagatgt
    85621 ttgggggcat gtggtttagc tccagttgtt actgtaaatg gtaaagttta tggtaaagtg
    85681 aaacctgacc aagtaagcga aatattagat acttatagag aattagaatt gaattgttaa
    85741 gaggggtgaa taaataatgc gtaaagtaaa ttcatttgat gaattaaaag ttttagctga
    85801 tgagctgaaa cctaatctta gcttaagaaa aaattattct aaggaaaatg tattaagaag
    85861 agaattactt gtttgttgtg atactggatg tacatcttca aacagtctag aaatagttag
    85921 tgaattagaa aatgaaataa agaaatcagg aatccaagat aaagttagtg ttcgtttgac
    85981 aggttgtttt ggtttctgtg ctcaaggtcc tattgtaaaa gtttatcctg ataacgtttt
    86041 ctacgtaaaa gttgagccat ctgatgctga aaagatagtt caaagccact taattagaaa
    86101 tacagttgtc gaggaattat tatatgagga agaatcttta agtaaaaaag ttgaagaaca
    86161 agaagaaatg tctttctaca aaaaacagtt acgtatagct ttaaaaaatt gtggtcttat
    86221 agacccagat agtatagaag aatatattgc caatgatggt tatttagctc taggcaaatg
    86281 tttaacttct cttactcctc aagaagttat tgcagaggta aaaacttctg gccttcgtgg
    86341 tagaggtggt gcaggtttcc ctactggctc taaatgggaa gctgcttcaa aaaatccagc
    86401 tggtccaaca gataaaaagt ttgttgtatg taatgctgat gaaggtgacc caggtgcatt
    86461 tatggataga tctgtacttg aaggtgaccc tcatagtgta ctagaagcta tggcaatatg
    86521 tggatacgct ataggttctg atacaggata catatacata agagcagaat atccaaaatc
    86581 tatagaaaga ttaaaagtag ccatagctca agctgaaaaa tacggtttac ttggcgaaaa
    86641 tatcttaggc actggattta actttaaatt agaattaaaa tatggtgcag gtgctttcgt
    86701 ttgtggtgaa ggtacagcgc taatgcattc aattgaaggt agacgtggtg aacctagaat
    86761 gaaaacttat agttcttcta agagtggtct atggaaatct cctacttgct taaataacgt
    86821 tgagactttt gctaacatac ctgcaattat acttaagggt ggagattggt ttgccaactt
    86881 aggtactgaa gattctagtg gtactaaagt ctttgctctt ggtggaaagg tagaaaatgt
    86941 aggacttgtt gaagttccaa tgggtactac tttaagagat attgtatttg aaattggtgg
    87001 gggaatccct gaaggtaaga aatttaaggc tgttcaaaca ggtggaccat ctggtggatg
    87061 tataccaact gaacatttag acactcctat agactttggt tcattaagtt ctatcggttc
    87121 aatgatgggc tctggtggta tgcttgttct tgatgaatct gactgtatgg tagatatagc
    87181 caagttcttc cttgagttta ctgttgaaga atcttgtggt aaatgtacac cttgtcgtat
    87241 aggtactaca agattattag aaatattaac taaaattaca gaaggtaatg gtactctaca
    87301 agatttagat gacctagaaa accttgctga aactatacaa actgcttcat tatgtggtct
    87361 tggtaaagct gctccaaacc ctgttttaag tactcttcaa tatttcaaag atgagtatat
    87421 agctcatgtt gtagataaga aatgtcctgc tggtaaatgt cagaacttac tttcttactt
    87481 tataacagat gactgtaagg gttgtacaaa atgttctaga gtgtgtcctg ctggttgtat
    87541 aacaggttct gttaaggaac aacatacaat agatacttct aagtgtctaa aatgtggtgc
    87601 ttgtatagat aattgtacat ttaacgctat aatcaagaaa taacttattt ggaggaggaa
    87661 taacaatgag tttagttaat ttaactatta atggcaaaca cgtttcagca cctagtggaa
    87721 cttctatctt agatgctgcg aaattaataa atataaaaat acacaacctt tgtcatttac
    87781 atatgaatga aatagataag cttgacactt gtgcttcttg tcgtgtttgt atggttgaga
    87841 cagagagagg attagttcca gcttgtggaa cagttataaa ggaaggtatg aaagttcaga
    87901 ctaatagtgc taaagcatta aatgctcgta gaactattgt tgaattattg ttatcagacc
    87961 atccacaaga ttgttttgtc tgtgaaaaaa atggagattg tgaattacag actatagctg
    88021 ctgatttagg agtaagaaaa ataagatatc aaggttctaa atcttttgca ggtaaagata
    88081 cttctactaa atctttagtt aaagaccatt ctaagtgtat attatgtaga agatgtgaga
    88141 ctgtatgtaa tgacatacaa acagtaggag cattatctgg agttaatcgt ggatttaata
    88201 ctttagttag tacattcttt aactctgata tggttgaaac agagtgtaca ttctgtggtc
    88261 aatgtatatc tgtctgtcca actggagcat taacagaagt agataatgta cctaaacttt
    88321 gggatgtttt aaataaaaaa gaaaagacta tagtagtgca agttgctcct gctgtaagag
    88381 ttgctattgg tgaagagttt ggattagaac cagggtcaat ctctacaggg aaaatggtag
    88441 ctgctttaaa agcacttgga tttgaacatg tatttgatac aaactttggt gcagatttta
    88501 ctataatgga agaagctact gaatttatag aaagaatcca aaaaggtgaa aatcttccta
    88561 tattaacaag ttgttgtcct gcatgggtaa acttcttaga gcacaattat ccagacaagt
    88621 taaacttagc atctagctgt aagtctcctc aaggtatgtt tggttctata gctaaaaact
    88681 actatgcacc taaaatatta ggaatcaatc ctgaagaact ttatgttgta tctgtaatgc
    88741 cttgtgtagc taaaaaatat gaagcttcaa gagaagaatt aagtgaatct ggaatactag
    88801 atgttgacct ttctataact actagagagt tagctaagat gataaaagaa gctgcaatcg
    88861 atttacctaa tcttcaagac caagattttg ataatcctct aggaaaatct acaggtgctg
    88921 catctatatt tggagcaagt ggtggagttt tagaagctgc cctacgtact tcatatgaga
    88981 agataactaa taagacttta gataatgtaa acttcactaa tgttagaggt cttaaaggaa
    89041 ttagggaagc aagtatagat gttgatggaa ctacagttaa tgtatgtatt gtaaatacac
    89101 ttaaaaacgc tagaaagatt atggataagg tcagaagtgg agaatgtaaa taccatatca
    89161 tagaagttat ggcatgtcct ggtggatgtg ttggaggtgc tggtcaacct tatcaccatg
    89221 gtaatactga aatagttgat agacgtgcaa atgcgttata tgaaatagat agaaataagg
    89281 ctattcgtaa atctcatgag aatcctgatt tacaagctat ttataaagac ttctttggag
    89341 aaccaaatag tgatgtagct cataaatacc ttcatactca ttactttgat aagagttgtg
    89401 tctatggaga gtgtccacaa gaatgtgctt gtgaagaagc taagtagtca taacaaagta
    89461 taaaatttat tcttaaaaaa ggattgtctt aaaatacttt tacgtaattt aagacaatcc
    89521 ttattaattt cacaaattaa attatatttc taaatagact tatttattaa ttaacctatc
    89581 tattaattaa agaatacctg ccattctcat aagttgtttt gcatttgtag ttttacatct
    89641 tccatctttt atcttatagt caaaatgaat tttatcgtct acatagtact ctgagaaatg
    89701 atagtttaca acttcaacat ctccgctacc tgaaaggtca catagctcaa agtcatgtgt
    89761 tgtcaccata cttataaccc agggttttga taatttcttt actgcctcac ttgctccaat
    89821 aattctatca gcagagttag ttcccttaaa aatttcatct actagaacca acattggcag
    89881 ttcttttaaa ctatattgaa tcattgattt tattctaaga atctcagcat agaaagttga
    89941 tattccttga cttacatcat cttgtattct catagaagta aatatagcca tacaggtcgc
    90001 atcaaaactc ttagcgcata ctggagcacc tgcatatgcc agcactaagt ttacaccgat
    90061 acttcttaaa aatgttgtct ttccagacat atttgaacct gtgataatac aagtctgaga
    90121 atttaattgt atcgaatttg ctatgacact tttttctgct attaatgggt ggtatacctc
    90181 ttcaactttc aatcttggag tatcttcata ttttattact ggaaaacatg tattttcctt
    90241 tacatgagaa acaactgtta aacttattag agcttcaaat tctccaatag attcaaacca
    90301 atctctaatt tgatttccat atataccttt ccatcgctcc aaagcttctg cacaatgata
    90361 gttccacatc aaaacaccac aggcaattat atatagtatc tgattatacc ttaggtttac
    90421 agcatttcct attgaattta attgtttaac acctcttgaa actccacttc ccttagaaag
    90481 tttatcttgt aattctttca aatatgcact ctcaaacgtc tctttttcta aaacctcaaa
    90541 cattttttca tatacttgta tacttctact aaatgaaaat aatggagcaa gagtttgcat
    90601 aagtcttgga tatccaaaac cactaaaccc taactggatt ataaaaagta aaaaaacagg
    90661 taaaacaggt aaaaatccta acatacaaaa tataaaagat aagattgttg cagttggtaa
    90721 accccaagta aaaatatgca tccattttgg tatatacaca ttcttatttt ctccatattc
    90781 tataaacgat tctatactac tatctgaatt gtttttttgc tctttaccaa ttatattact
    90841 gagtgtttga atatgaatag aaaaatcatc ttttgataaa agttctttta tagctctctg
    90901 tctttctaaa attatattct catctggatt ttcattccac aaatatttgg ctaaaaaatt
    90961 tttcccataa gatgtattag ctacacagat atattgatac aatgatgctc ttccaaataa
    91021 gtctaaatct tttgcttgcg aattttcatc ttttaagtat tctttaccat ctattttaaa
    91081 ttccttccaa ccatctccaa atctcttaat atacttattt aatacttcgc tcttactttt
    91141 aaaatatgtt tcttcttctt ttactttact atgaataact aagagtgcta taaatattat
    91201 tattgataaa actccaacaa aaatacctac tatattttta ttataaaatc caatagcaag
    91261 ccctgctatc actatcaaaa aagatattaa tcttgctgta cttatgttat ttgacttttt
    91321 acttaagagc aattcttttt cagtactact ttctaaaagt ttactaaaat attctattcc
    91381 cataaaaaat tccaactttc ttgttatata atgaaactca aatataaaat taatctagta
    91441 ttattataat ctaacactgt actttaaatt atccttatat aatatatctt attaataaac
    91501 attatatata caacttgatt ttaatttata aggtgtagga cataatccta atcaatattt
    91561 aattaaatga tgtgtagtcc actattattc agtactactt agtatcgatt aattttgata
    91621 attttttagt aaattcttga gctgcatcgt atcccattcc tctttgtcta aaattcattg
    91681 ctgctacttc taatatcata gcagtatttc ttccaggttt aacaggtaca actaatttct
    91741 ctacattaac accaagtatt tcttcatatt ctttatccag tcctagtcta tcataatact
    91801 tagtttcatc ccaagcttct aaatgaacta ctaaatctat cattttagaa tttttgattg
    91861 cccctactcc atataaactt ctaacatcta ttattcctat tcctcttatc tcaagaaaat
    91921 gtcttattag ttcaggtgac tgacccacaa gcatactttc atctaatttt cttatttcta
    91981 cagcatcatc tgcaactaat ctattacccc tttgaatcag ttctaatgct gtttccgact
    92041 ttcctatact actttcacct tttatcagta cacctatacc ataaatatct actagcacac
    92101 cgtgaattgt tgtatgtggt gctaacttat tatccaaata gtttgacagt ctatttataa
    92161 gtctagtggt atttctattt gtgctcaaaa gtattctttt atatttttta gccttttcaa
    92221 ttacatctgg cttgatttcc aagtctctag aaattattaa cgctggtatt tcatatgaga
    92281 aaaatctatc tagtacttct tcccgaatat gactatccat taatccaaaa aaattatatt
    92341 ctgtctttcc aactatttgt attctttcat atgcaaaata atcaaaatat cctgcaaact
    92401 gtaatcctgg tctatttata tcttgagagt agacataata atctacatca tcaggcatgt
    92461 atactacttt taacttcaac tcttttatta tctctcttat tgatacttta ttgctagttt
    92521 ccattaattt tcttcctttc taaaaaaatt ataaacatta tctgcaatgc ttttattcat
    92581 tccatctact tctaaaagtt cttccatagt agcttttttt atagcatcta catctttaaa
    92641 gtgatttaat aatgatttct ttcttttttc acctattcct tgtatatcat ctaagataga
    92701 ttttgtcaaa gctttatttc ttagacttct atgatatgtt attgcataat tatgaacttc
    92761 ttcttgtata ctagcaataa atctatataa attagttgtt ttatctagtt ctatttcttt
    92821 ttctttgcat atcaatcctt tagttctatg cttatcatct ttgtacattc cccaaagagg
    92881 tatatctaca tcattgagtt ctagtacttt ttttactgca ctaacttgtc cttttccacc
    92941 atccaacaat atcaagtcag gtaaatttcc atgttttaat cttctatcta caatttcagc
    93001 catagaatca taatcattag gtccaataac tgtctttatt ttatatctcc tatattcctt
    93061 cttatctttt ttagcattcg tatacactac catagaacca atcgaatcta caccttgtat
    93121 atttgatata tcataagcct ctattcttat tggcaacttt tctagattta atatttgctt
    93181 taattcttcc aatgctccaa tacttttttc atattttctt ttattcatat cagaaaattt
    93241 ttcaagatat tctacagcat ttttcctaac catttcaact aagcttttct tctctccctt
    93301 ttgaggaact cttattgtta ctttttgccc ctttttagat gacagccatt cttctaatat
    93361 agaactatct tctatctcat cttctataat taattcttta ggtatatact cttgttcatt
    93421 ataaaattgt tttacaaatg aacttaatat agaagctctt ggactatcca ttaccccttc
    93481 taatataaaa tgttccctac ctacaatctt accatttctt ataaaaaata cctggacaca
    93541 agcttcatta tgagctcttg ccattgcaac cacatcttgg tttaagtcac ttacagtagc
    93601 atctattttt tgtttctgta tcatctcttc aaggctcttt atcttatccc tatatacagc
    93661 agcctcttca aatctaaagt tcatagaact ctcattcatt ttttccttta aaagttccat
    93721 caatttttct tctttaccag ataaacacat tataatctct tctatcattt ttccatactc
    93781 ttctttagaa acatttcctg tacaaggtcc taaacattta tttatgtgta gatttaaaca
    93841 aggtcttgtc ttattcttta ttgctttatc tatatctatt ttacagcttc ttattggata
    93901 cgtactactt ataagctcca aggtatcatt cacagcagtt atattggtat agggcccaaa
    93961 atactttgcc ttatctttaa gaactcttct aacttttaag attcttggat aatcttcatt
    94021 cgtagttact tttatatatg gataagtttt atcatctcta agcactacat tatactttgg
    94081 tctgtatctt tttattaaat tacattcaag tattaatgcc tctaactctg aatccgttat
    94141 aatatactca aacttatata tattcttaac catagatttt actttagatg tatggttttt
    94201 tgaagattga aaatactgcc ttaccctatt ttttagagat atggcttttc ccacatatat
    94261 tatatggtcg tatttgtctt tcattaaata tactccgggc tcagaaggta atttttttaa
    94321 atgctcttgt atatcaaaca tctcgccctc caaaacatta aattttatac taaaattata
    94381 ccctaaaata tgaattttaa aaagttatac aatttccagt gctttaaaca agttaaagcc
    94441 catattccct tctataattg atttgaaaac aatgttaacc atgaagagta atatggactt
    94501 tactttgtat tactatttac aacttatatt tgactaattt acttataaca aaacagttta
    94561 tcgtatatct gctctaaaat ttttcacaat ttatataact taagaaaaat atttctttaa
    94621 gaattgacct gtataagaac catcaacttt agatacttct tttggtgttc cagttgcaac
    94681 tatagttcca cccttatctc cgccttctgg tcctaagtct attacataat cacaagtctt
    94741 tataacatct aaattatgtt ctatgactat aattgtattt cctgtatctg ctagtctttg
    94801 taatacttgg attagcttgt ctacatctgc catatgaagt ccagtagtag gttcatctaa
    94861 tatatacaaa gtctttcctg ttggtctttt actaagttca gcagctaatt ttatacgttg
    94921 tgcttctcct cctgataatt gagttgaagg ctgtcctaat ttaatatatg aaagtcctac
    94981 atccattaaa gtttctaatt ttctttttat atttggtata ttttcgaaaa actcaacagc
    95041 ttcttccaca ttcatatcta atatatcaga tatagtttta tctttgtatt ttacttgcaa
    95101 tgtttctcta ttgtatcttt cacctttaca aatttcacaa ggaacatata catctggtaa
    95161 gaaatgcatt tctattttta ttattccatc acccttacaa gcttcacatc taccaccttt
    95221 tacattaaaa ctaaatctac cttttttata acctcttgct ttagcttcat tagtagatgc
    95281 aaaaatatct cttataaaat caaatacacc tgtataagta gcaggatttg aacgaggtgt
    95341 tcttccaata ggactttggt ctatatttat aactttatct atactttcaa ttccttttat
    95401 ctccttatgc ttaccagctc tttgcttaag tctattcact ttacttgcaa cacctttgta
    95461 aagtatatcg tttattaatg tactttttcc agaacctgaa acacctgtta tgcatgtaaa
    95521 cttacctaat gggaacttta catcaatatt tttcagatta ttttctgatg ctttaactat
    95581 ttctataaaa cttccatttc cttctctagt agtctcagga agcaatattt gttttttacc
    95641 acttaaatat tgacccgtta ttgagttctc attttcaatt atttcatcta aagtaccctg
    95701 tgcaactatt tcaccaccat gtatacctgc acctggacca atgtctataa cataatcagc
    95761 ttctctcatt gtgtcttcat catgctctac aacgattaag gtatttccta aatctgttag
    95821 atgtctaaga gtagttataa gcctatcatt atctctttgg tgaagcccta tacttggctc
    95881 atcaagtaca tataaaactc caaccaatgc agaacctatt tgagtagcta atcttatacg
    95941 ttgcgcttct cctcctgata aagttccagc ttttcttgat aaatttaaat aatcaagacc
    96001 tacatctctt aagaacatag ctcttccttt tatctctttt ataatctcat gagctataaa
    96061 tctctgtttt tctgtaagat ttatattttc cataaagtca atcaattcat ttacagataa
    96121 gtttgtaact tccattatat tttttccatc aactaaaaca gataaaactt cctttttaag
    96181 tctcatccct ttacactttg gacatggtct ctctgccatg tattcttcaa tcttatctct
    96241 catgtaatct gaattagttt ctctatatct tctctctaag tttactatta caccttcaaa
    96301 gtatgattta taatttctag ttcctccaaa ttttgactca taagtaaact ctacaattat
    96361 attatcttta ccataaagta attcctccac aaaatcatct ggtaaatctt taaacggtgt
    96421 tgtcaaagat acattaaaat gctctgctaa actttttatc attttactgt aataagtgtc
    96481 atcattaaca cctgttgaac cccatgctgc cactgcacct tgttttattg ataaatcttt
    96541 gtttggtact actaattctg ggtcaacttc tttactttcc ccaagaccat tacaaacatc
    96601 acaagcacca aagggagcat taaatgaaaa cattcttgga gataattctt ctatgccaat
    96661 tccatgttct gggcatgcaa atttagttga aaaaagaatt tcttctccat ctattatttg
    96721 ggctattaca agaccatcag atagcttaac tgcagtttca attgaatctg ctagtcttga
    96781 ttctattcca tctttaatta ctattctgtc cactacaact tctatattat gtttttgatt
    96841 tttatttaag attatatcat cactaacttc atagttttca ccattaactc taactcttac
    96901 aaatccttct tttttgatat tatcaaagac ttttttatgt gtaccttttt gtcctctaac
    96961 aattggtgaa agaatttgta atttcgttct atctggaaaa tctagcattt tatcaactat
    97021 ttcttgaata gtcatctgac ttattttttc accacatgta gggcaataca catctcctac
    97081 tcttgcaaat aatagtctca aataatcata tatttctgtt actgttccaa ccgtagaacg
    97141 tgggtttttt gaggtagtct tctggtcaat tgagatagct ggtgataaac cctctatata
    97201 ttctacattt ggcttttcca tctgacctaa aaattgtctt gcataagcag atagactttc
    97261 tacatatctt ctctgtcctt ctgcatatat tgtatcaaat gccaaagaag acttcccaga
    97321 ccctgacaaa cctgtaaaaa caataaattt atctcttggt aattctaaat ctatattttt
    97381 taaattatgt tcttttgctc ctcttattat aattttatct tccataatgt tgtttttacc
    97441 tttctatttt tccaattttt cttctaattg tttcacttta tctctaagtt ctgctgctct
    97501 ttcaaattgt aagttttgag cagcttccat catctctgat tttaatttat ctatattagc
    97561 tcttatttcg tctttatcat ctgtatcaga tattccatat acaacttctt cctctgcaac
    97621 cttagtagct tctatactat ctcttatatt tttctgtata gttttaggaa tgatattgtg
    97681 ctcttcatta tataaatttt gtatatctct tcttctcttt gtttcatcaa tagcattttg
    97741 cattgagtca gttatcctgt ctgcatacat aattactctt ccattttcat ttctagctgc
    97801 acgtccaata gtctgtatta aagctgtttc ggaacgtaag aaaccttctt tgtctgcatc
    97861 taaaattgct ataagtgaaa cttctggtat atccaatcct tctctaagta agtttattcc
    97921 tacaaggaca tcaaacttcc ctaatcttaa atctcttatt atttctgttc tttctaatgt
    97981 aactatatct gagtgtaaat atttaacttt tatccctatt tctttaagat agttagtcaa
    98041 gtcctcactc atttttttag ttaaagtggt tataagcact ctttcatttt tttctatagt
    98101 tttagttatt tctcctacca aatcatctat ttgattattt ataggtctta cttctacaat
    98161 tgggtctaaa aggccagttg gtcttattat ctgttctgct attgtctctg agtgttgaat
    98221 ttcataaggc cctggtgtag cacttacaaa caacatttga tttatatttc cttcaaattc
    98281 ttgaaaattt aatggtctat tgtcaagtgc agaaggtaat ctaaaaccat tctctataag
    98341 tgatgttttt ctagacctgt ctcctgcata catacctctt acttgtggta tagtgacatg
    98401 tgcctcatct acaattatca aataatcatc tgggaagaaa tccattaaag tatatggtct
    98461 ttctccttct gaccttcctg taatatgtct agaatagttc tctatcccct gacaaaatcc
    98521 tatttccttt aacatttcta tatcatactt agttctttgt tcaattcttt gtgcttctaa
    98581 tagcctatca ttttccttga aaaatttaat ttgttcttga agctcgtttt ctatttctac
    98641 aattgccttt tcaattcttt ctggcgttgt tacatagtga gatgctggga atataacaac
    98701 atgatttcta gtaccaacaa tcttccctgt tacataatca atttctgtta ttctatctac
    98761 ctcatcacca aaaaattcta ttcttattgc tttttcatca ttactagctg ggaatatttc
    98821 tagtatatct cctctaactc taaaggtacc tcgtgtaaag tttatgtcat ttctttcgta
    98881 ttgtatctct attaaccttt ttattacatc atctctgtct ctttgcatac ctggtcttat
    98941 agataacata agttctttat agtcttttgg gtcaccaagt ccatatatac aagatacaga
    99001 tgatattatt atagtatctc ttctctctaa aatagatgct gtagcagaat gtcgaagttt
    99061 atctatttca tcatttatgc ttgcatcttt ttctatataa ttgtcactgt gagctacata
    99121 tgcttctggt tgataataat catagtaact tacaaagtat tcaactgcat tgtctgggaa
    99181 aaattcttta aactcactgt aaagttgtgc tgctagagtt ttattatgag ccattattag
    99241 ggtaggtttc ttaacttgtt gaatgatatt tgccattgtg aatgtttttc ctgaacccgt
    99301 tacacctaat aatgtcgaaa atttctcatt tctatttata gaatccacta ttgatttaat
    99361 agcttctggt tggtcacctg taggtttgaa gtctgatttt attttaaaat ccatagtatc
    99421 actccttttt tcgaacatta gttcctgaca tgtattatta tatcatacct ttataaaatt
    99481 gtttaagtta ttttaaatat tttacccatt taaattttac ttaaatctaa aacataaaaa
    99541 aagacttcta tctttgcaat agaagtctat aattcatgga aaataaatac tttattcaac
    99601 ttacataata aaatcaaatg cttctttttc aactccatct aattttaaat taaacatttt
    99661 taatatagtt gctgcatgtg ctctcaggtc tccttcttct ataaccacac cacctttaat
    99721 atttggtcca aaagctataa atggaggtct attaccttta gtaggtaaat gaccatgtgt
    99781 cgcaacagca aacttgtaat cttttatatc tgaatctttt ataatacttc cttcgaaatc
    99841 atttccaaat gaaattcctt caagaccttc aattaccatt tcaaaatcac cttcaagacc
    99901 aaggttagat gcttcctctt tggtaaaaat attctctatg aataattctt ctttaaattc
    99961 ttcaagcagt tctcttaact ctgttaacct atctaagtta tttactatta tttgagctga
   100021 tattccagaa gaatttacat aggcatcaaa atccttaact tctccatttt caacttttat
   100081 aaatccattt gatttaagca agacatttgg atttataact ttatcaactc taagatgacc
   100141 atggtcccca agtattataa aatttgtatc tttgtatgta cctgcatcct ttgtagcctg
   100201 aataatatct cctagccatt tatcattcat ttttaatgct ttatctactt cgtcattaaa
   100261 taagccataa ttgtgtcgtg tatggtccaa tgtagctaaa tgtattagca ttaaatctgg
   100321 tttatattcc tttattatat ctactgaaca gtttactcca aataaatcca tgttgggttc
   100381 taatttccaa tcgatataat gacaatgtct attgtaaata ccgtccatta taaccttact
   100441 ggaacttcgt tcaaaagttt ctcttgggtc atcttctcta ctaggagccc atatttctgc
   100501 aatattataa tctatatttg gatttgctcc cattacaggc cataatacac tagaggtagt
   100561 tagattattt tcttttgcta catctataat agtttttgct tttatgtcct ttgagtacca
   100621 gtaccagtct ttattttctt tatttgggtc aaatttttca ttatgatata taccatgttt
   100681 tttaggatat actcctgtaa ccatactagt atgacacgga tatgtcagag tgggataaac
   100741 acaatttata ttttttacta tggagcaatt ttctagtata gatttaaagt ttggtagatt
   100801 ttttacaaat tccaagtctt tttcaaataa agaatctact gataatacta taacctttgt
   100861 catttacatt acctcttcat ttatatatta ttgaatactt ttaatcaact tacaagtcta
   100921 tataattatt tatttgtttt atctattact gcttgcattg cagaagttcc tttgcttact
   100981 acatcttttg gatttgcatt taaatcagta agcccctttt taatctcatc cattagctta
   101041 tcagttgctt ccttgtatcc atttaacatt ggaagtgcaa cagcatattg taattggtca
   101101 gttgcaacct tatattgagg tttttcttta tataatgttt gtaacttttc tgagtttgtt
   101161 gcatcttcag ttgttggtaa gtatcctgtt gatgaactaa actgaacgat tttatctgtt
   101221 tgtgtcatcc attctataaa ttctgcactt gcttcttttt ctttatctgt tccaccttca
   101281 agcataacta gattagctcc acctgtagga gttgcgtatt tttgattttt tggtaagaat
   101341 gctgttgata tttcaaactt accttctgtt tgttgcataa gtcctgctaa atctgctgtt
   101401 gatgtaaata tcatacctac tttaccattt acaaaatctt gttttgctac gtcccaggca
   101461 ttataatctt ctccaggagg cattttcatt attccagctt tcatcatatc ttgccagaac
   101521 ttaactggtg cttgtcctgc ctcattatta aatgctactt ttttaccatt agttatttct
   101581 cctccacttt gcattaccat agcttcatag aaccaaatat ctattggtgt ttcaaaacca
   101641 taagtatctt ctgatttatt tgtcatttta ctagcataat ccttaagctc ttcccatgat
   101701 tttggacctg aagggtcaag acctgcttta gttgccatat ctttatttat gtataatatt
   101761 ggtgtacttc tattaaatgg cataccaaca aacttatcct cataatatga atttgttaaa
   101821 agaccagaaa agaatctttc ttgatatgtt ttatcaaaat atttactcat atctactaaa
   101881 gcacctgctg atgcgaatgt acctgtttga gttatttcta ataatgttac atctggttgg
   101941 tctttagatg ttaaggctgc ctgtagtttt gttgcatttt catagtaatc tttctgtttt
   102001 aacatattta cttgtacatc cttatgttca ctattaaatt ggtcaaccat tttttgagca
   102061 gtttctccat tagttccacc taaaggagcc cagaaatcta tcttcgtaat attgctatca
   102121 gtcttttctc ctttagatgc atttgcatca ttagaatttg aacacccaac taaagaacat
   102181 aataataatg ttattgctaa tcctgttgct aattttcttt taagcttcat tttcatcctc
   102241 caaatttagt ctttaatttt ttattttatt cctgaataca taaaagcttc ttttactttt
   102301 ttattcgcaa atatataaac aagaattatt ggtaaaatta gaattatatt tcctgccatt
   102361 attacattcc atgcagtaat tccttctgaa gatttaagga gcgcaactcc tattggtaaa
   102421 gttcttattg catctgtatt tgtcattact aagggccaga aataattatt ccaatggtat
   102481 ataaaattaa ataatataaa tgttattact gctggttttg ccataggaac cattattcta
   102541 aacattattt ttaagtcaga agcattatct agtttagctg cttcaataat ttcatctgaa
   102601 atctgcataa aattctgtct aagcagaaat atcccaaatg aagatgtcat aaatggaatt
   102661 ataagtggaa cataagtatt tataaatcca aacttactca tcatcatata gactggtaga
   102721 aaagttactt gtggtggaat cataagtcct acaagcaaaa gcccaaatag cattttttca
   102781 cctttaaatt taatcttaga aaatgcatat gctgctggaa ccataactaa aaattgtaca
   102841 attaaaacac ttcctgttac aaatacactg tttagcaaat atctaaaaaa tggacctgaa
   102901 tgccatgctg tactataatt ttctaacact atatcatttg gtataatagt gggcggtaat
   102961 tgcatcattt cttgtggatt tttaaaggat gtagatagca tccatataaa agggaatata
   103021 aatattgcag ataaaagtaa tagaattaat atttctattg attttgatgc tatttttttt
   103081 atatcaactt tgtctttttt gataggttgt ataatatcca ttgtctcctc cttttggata
   103141 aaccttataa tatctacttt ttctttattg ataatgaact ttctttgata agagtctaaa
   103201 gttagcatat gtagcaatca taactatagc taacaaaaac atactcgctg cactcgcttg
   103261 ccctatgtca tagtatataa atccagactc atatatataa taagataaca tgttagttga
   103321 acccactggc ccaccttgtg tcattatact tatctcatca aatacttgaa aagatgcagt
   103381 tgttgttgtt atcataagaa aaaatattgt tggtgataat agtggcaatg tcactttaaa
   103441 gaaattcgtt attttgtttg cattatctaa tttagatgct tcatatatat agtttggtat
   103501 ggatttaaga cctgataaca ctattaagat attatatcca agagtcttcc atattcctat
   103561 tagaattagt gacattaaag ctgaactttc acttgaaagc cactttgatg ttggtaaatg
   103621 taatgtcgat agtatccagt ttaaaaagcc atattgtggc tccataatcc aaagccacaa
   103681 tacagctaca gatactagtg aaattacatg tggtgtaaag attatacttt gcgttaaatt
   103741 gtgtattata gaatttttat ttagccaaac agcaagtact atggctagtg atatagatat
   103801 agtcaccata aaaaaagtat aaattgcagt gtttgttata gtttgtataa actctggatt
   103861 ctttaaaagt tctgtataat tgtttaagcc tacattttca aaggttgaac taagaagact
   103921 accagatttg aagcttagag atatcgtctt aattattgga tagactataa acaataaagt
   103981 tcctataata gcaggtgtta tatataaata tgctttatta aattgctttt tcataaatat
   104041 ctcctttgat tttagaatac atacttttac atacttttac atcttctatt ctttctccat
   104101 ttttatcaaa ataatataaa tctttttcta atacacttat tcttaaagtt cttccaatat
   104161 caaatatatc attttccgtt ttgactctta tctctctatt aagaacagat aaacaatata
   104221 tagtttcatt tcccaacact tctttagtta caacttttcc aaatagacta aatacatcat
   104281 catttttccc atctatgttt atactctcta gtgttgcttt ttctggtctg aatccaaatt
   104341 taatatcatt ttttatatct agtatgttca tacaaggact ccctataaat tgagctacaa
   104401 ataaattttt aggattttga tatatcttct ttggagaacc tatttgcatt atctttcctt
   104461 tgttcattat aactatctta tctgccattg acattgcctc aacctggtca tgagttacat
   104521 aaacaaatgt agactttagt ttttgatgta attgtattaa ttctgttctc atttgatttc
   104581 ttaatttagc atctaaattt gacaatggtt catccattaa aaataccttt ggatttttaa
   104641 ccatagctct agcaagtgcc actctttgtc tttgacctcc agataagttg cctggtttca
   104701 catttaaata ttctctcaat cctactacat caataacttc tgttataagt ttttctcttt
   104761 tttctttttc tacttttttg tttttaagac caaattctat gttttctcta acagtcatat
   104821 gtggatataa tgcataattt tgaaatacca tagctatatc ccttttacta gcatccgttt
   104881 ttgtaacatc attttcttca atcttcaacc tacctgaagt tatatcttca agacctgcaa
   104941 tcattcttaa cgtagtagat ttaccacatc cacttgggcc taccaatact gtaaaagaac
   105001 catcttctat agtaagattt aaattttcta taactgtatt ttttttatta taagattttg
   105061 ttacattttc tagtcttata tctgccattt ttatcctccc aaaatattta atttaattct
   105121 acgatttact tatagattaa taatactctt aatttgtaaa ggatatatta agacaaagtg
   105181 aatatatggt aaacttatta aaataaaggt taaattataa acattttttg ttaaatatac
   105241 tgaagttaag atgatataca gcttatgtta gctataattt tctatatttt tcatattcat
   105301 ggtttgctat cacaatgtaa ttttaattta tgaagagtca tataaaacta tttatttttt
   105361 atataatttg gtttgaaagt aaaaaagacc tctatggggg atagaggtct ttaagtgggg
   105421 gagattgagt aatttatttt tgctatatta agttttacaa ttatattatg tcccaaattt
   105481 acaaggttta aacataaaaa ataaaaattt tttaattttt ataaatttac ccaaaaaa
//
LOCUS       DAMJYX010000002        96494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJYX010000002 DAMJYX010000000
VERSION     DAMJYX010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 96494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.002"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            39..155
                     /gene="rrf"
                     /locus_tag="PC479_000091"
     rRNA            39..155
                     /gene="rrf"
                     /locus_tag="PC479_000091"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            275..466
                     /locus_tag="PC479_000092"
     CDS             275..466
                     /locus_tag="PC479_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2802682.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            621..2030
                     /locus_tag="PC479_000093"
     CDS             621..2030
                     /locus_tag="PC479_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2802683.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2161..2850
                     /locus_tag="PC479_000094"
     CDS             2161..2850
                     /locus_tag="PC479_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2802684.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2853..3788
                     /locus_tag="PC479_000095"
     CDS             2853..3788
                     /locus_tag="PC479_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2802685.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3785..4678
                     /locus_tag="PC479_000096"
     CDS             3785..4678
                     /locus_tag="PC479_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2802686.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4756..5502
                     /locus_tag="PC479_000097"
     CDS             4756..5502
                     /locus_tag="PC479_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2802687.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5503..6336
                     /gene="rsmI"
                     /locus_tag="PC479_000098"
     CDS             5503..6336
                     /gene="rsmI"
                     /locus_tag="PC479_000098"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2802688.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6359..7063
                     /locus_tag="PC479_000099"
     CDS             6359..7063
                     /locus_tag="PC479_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2802689.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7251..7679)
                     /locus_tag="PC479_000100"
     CDS             complement(7251..7679)
                     /locus_tag="PC479_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2802690.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7716..9146)
                     /locus_tag="PC479_000101"
     CDS             complement(7716..9146)
                     /locus_tag="PC479_000101"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2802691.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9278..9862
                     /locus_tag="PC479_000102"
     CDS             9278..9862
                     /locus_tag="PC479_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2802692.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9878..10399
                     /locus_tag="PC479_000103"
     CDS             9878..10399
                     /locus_tag="PC479_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HDF2802693.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10465..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10795..12732
                     /gene="metG"
                     /locus_tag="PC479_000104"
     CDS             10795..12732
                     /gene="metG"
                     /locus_tag="PC479_000104"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2802694.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12762..13532
                     /locus_tag="PC479_000105"
     CDS             12762..13532
                     /locus_tag="PC479_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2802695.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13870..14301
                     /gene="phnG"
                     /locus_tag="PC479_000106"
     CDS             13870..14301
                     /gene="phnG"
                     /locus_tag="PC479_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2802696.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14316..14891
                     /gene="phnH"
                     /locus_tag="PC479_000107"
     CDS             14316..14891
                     /gene="phnH"
                     /locus_tag="PC479_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2802697.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14894..16009
                     /locus_tag="PC479_000108"
     CDS             14894..16009
                     /locus_tag="PC479_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2802698.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15999..16868
                     /locus_tag="PC479_000109"
     CDS             15999..16868
                     /locus_tag="PC479_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2802699.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16861..17727
                     /locus_tag="PC479_000110"
     CDS             16861..17727
                     /locus_tag="PC479_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2802700.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17768..18481
                     /locus_tag="PC479_000111"
     CDS             17768..18481
                     /locus_tag="PC479_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2802701.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18482..19651
                     /locus_tag="PC479_000112"
     CDS             18482..19651
                     /locus_tag="PC479_000112"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2802702.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19655..20491
                     /locus_tag="PC479_000113"
     CDS             19655..20491
                     /locus_tag="PC479_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2802703.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20492..21487
                     /locus_tag="PC479_000114"
     CDS             20492..21487
                     /locus_tag="PC479_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2802704.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21505..23193
                     /locus_tag="PC479_000115"
     CDS             21505..23193
                     /locus_tag="PC479_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2802705.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23205..24272
                     /locus_tag="PC479_000116"
     CDS             23205..24272
                     /locus_tag="PC479_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2802706.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24756..24872
                     /locus_tag="PC479_000117"
     CDS             24756..24872
                     /locus_tag="PC479_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF2802707.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            25036..26103
                     /locus_tag="PC479_000118"
     CDS             25036..26103
                     /locus_tag="PC479_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2802708.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26295..26825
                     /gene="rnmV"
                     /locus_tag="PC479_000119"
     CDS             26295..26825
                     /gene="rnmV"
                     /locus_tag="PC479_000119"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2802709.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26996..27865
                     /gene="rsmA"
                     /locus_tag="PC479_000120"
     CDS             26996..27865
                     /gene="rsmA"
                     /locus_tag="PC479_000120"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2802710.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27931..29193
                     /locus_tag="PC479_000121"
     CDS             27931..29193
                     /locus_tag="PC479_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802711.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29344..30459
                     /locus_tag="PC479_000122"
     CDS             29344..30459
                     /locus_tag="PC479_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2802712.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30645..31541
                     /locus_tag="PC479_000123"
     CDS             30645..31541
                     /locus_tag="PC479_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2802713.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31611..32408
                     /locus_tag="PC479_000124"
     CDS             31611..32408
                     /locus_tag="PC479_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802714.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32463..33815)
                     /locus_tag="PC479_000125"
     CDS             complement(32463..33815)
                     /locus_tag="PC479_000125"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2802715.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            34034..34912
                     /gene="purR"
                     /locus_tag="PC479_000126"
     CDS             34034..34912
                     /gene="purR"
                     /locus_tag="PC479_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2802716.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            35068..35346
                     /gene="spoVG"
                     /locus_tag="PC479_000127"
     CDS             35068..35346
                     /gene="spoVG"
                     /locus_tag="PC479_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2802717.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35559..36938
                     /gene="glmU"
                     /locus_tag="PC479_000128"
     CDS             35559..36938
                     /gene="glmU"
                     /locus_tag="PC479_000128"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2802718.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            37033..37983
                     /locus_tag="PC479_000129"
     CDS             37033..37983
                     /locus_tag="PC479_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2802719.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38821..38906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            39068..39589
                     /locus_tag="PC479_000130"
     CDS             39068..39589
                     /locus_tag="PC479_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2802720.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            39740..41416
                     /locus_tag="PC479_000131"
     CDS             39740..41416
                     /locus_tag="PC479_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2802721.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41428..42636
                     /locus_tag="PC479_000132"
     CDS             41428..42636
                     /locus_tag="PC479_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2802722.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42651..44363
                     /locus_tag="PC479_000133"
     CDS             42651..44363
                     /locus_tag="PC479_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2802723.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44356..45264
                     /gene="pilO"
                     /locus_tag="PC479_000134"
     CDS             44356..45264
                     /gene="pilO"
                     /locus_tag="PC479_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2802724.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45291..45857
                     /locus_tag="PC479_000135"
     CDS             45291..45857
                     /locus_tag="PC479_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2802725.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45894..46421
                     /locus_tag="PC479_000136"
     CDS             45894..46421
                     /locus_tag="PC479_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2802726.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46442..47980
                     /locus_tag="PC479_000137"
     CDS             46442..47980
                     /locus_tag="PC479_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802727.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            48080..49162
                     /locus_tag="PC479_000138"
     CDS             48080..49162
                     /locus_tag="PC479_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2802728.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            49143..49925
                     /locus_tag="PC479_000139"
     CDS             49143..49925
                     /locus_tag="PC479_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2802729.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            50027..50677
                     /locus_tag="PC479_000140"
     CDS             50027..50677
                     /locus_tag="PC479_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2802730.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50691..51251
                     /locus_tag="PC479_000141"
     CDS             50691..51251
                     /locus_tag="PC479_000141"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2802731.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51268..54654
                     /gene="mfd"
                     /locus_tag="PC479_000142"
     CDS             51268..54654
                     /gene="mfd"
                     /locus_tag="PC479_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2802732.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54685..55680
                     /locus_tag="PC479_000143"
     CDS             54685..55680
                     /locus_tag="PC479_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2802733.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55804..56343
                     /gene="spoVT"
                     /locus_tag="PC479_000144"
     CDS             55804..56343
                     /gene="spoVT"
                     /locus_tag="PC479_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2802734.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56463..58076
                     /locus_tag="PC479_000145"
     CDS             56463..58076
                     /locus_tag="PC479_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2802735.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58183..59631
                     /gene="mazG"
                     /locus_tag="PC479_000146"
     CDS             58183..59631
                     /gene="mazG"
                     /locus_tag="PC479_000146"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2802736.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59720..59998
                     /locus_tag="PC479_000147"
     CDS             59720..59998
                     /locus_tag="PC479_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2802737.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            60070..60312
                     /locus_tag="PC479_000148"
     CDS             60070..60312
                     /locus_tag="PC479_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2802738.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60374..60628
                     /locus_tag="PC479_000149"
     CDS             60374..60628
                     /locus_tag="PC479_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2802739.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60629..61162
                     /locus_tag="PC479_000150"
     CDS             60629..61162
                     /locus_tag="PC479_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802740.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61168..61452
                     /locus_tag="PC479_000151"
     CDS             61168..61452
                     /locus_tag="PC479_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2802741.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61565..62485
                     /locus_tag="PC479_000152"
     CDS             61565..62485
                     /locus_tag="PC479_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2802742.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62575..64947
                     /gene="spoIIE"
                     /locus_tag="PC479_000153"
     CDS             62575..64947
                     /gene="spoIIE"
                     /locus_tag="PC479_000153"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2802743.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65298..67190
                     /gene="pepF"
                     /locus_tag="PC479_000154"
     CDS             65298..67190
                     /gene="pepF"
                     /locus_tag="PC479_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2802744.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67304..68269
                     /gene="galU"
                     /locus_tag="PC479_000155"
     CDS             67304..68269
                     /gene="galU"
                     /locus_tag="PC479_000155"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2802745.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68769..70373
                     /gene="rho"
                     /locus_tag="PC479_000156"
     CDS             68769..70373
                     /gene="rho"
                     /locus_tag="PC479_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2802746.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            70526..70726
                     /gene="rpmE"
                     /locus_tag="PC479_000157"
     CDS             70526..70726
                     /gene="rpmE"
                     /locus_tag="PC479_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2802747.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70871..71764
                     /locus_tag="PC479_000158"
     CDS             70871..71764
                     /locus_tag="PC479_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2802748.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71782..72630
                     /gene="prmC"
                     /locus_tag="PC479_000159"
     CDS             71782..72630
                     /gene="prmC"
                     /locus_tag="PC479_000159"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2802749.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72674..73738
                     /gene="prfA"
                     /locus_tag="PC479_000160"
     CDS             72674..73738
                     /gene="prfA"
                     /locus_tag="PC479_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2802750.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73867..74547
                     /locus_tag="PC479_000161"
     CDS             73867..74547
                     /locus_tag="PC479_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2802751.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74576..75616
                     /locus_tag="PC479_000162"
     CDS             74576..75616
                     /locus_tag="PC479_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2802752.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75633..76082
                     /locus_tag="PC479_000163"
     CDS             75633..76082
                     /locus_tag="PC479_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2802753.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            76099..76551
                     /gene="rpiB"
                     /locus_tag="PC479_000164"
     CDS             76099..76551
                     /gene="rpiB"
                     /locus_tag="PC479_000164"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2802754.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76587..77216
                     /gene="upp"
                     /locus_tag="PC479_000165"
     CDS             76587..77216
                     /gene="upp"
                     /locus_tag="PC479_000165"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2802755.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            77511..78326
                     /locus_tag="PC479_000166"
     CDS             77511..78326
                     /locus_tag="PC479_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2802756.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            78552..78989
                     /locus_tag="PC479_000167"
     CDS             78552..78989
                     /locus_tag="PC479_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2802757.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            79174..79401
                     /locus_tag="PC479_000168"
     CDS             79174..79401
                     /locus_tag="PC479_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2802758.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            79406..79792
                     /locus_tag="PC479_000169"
     CDS             79406..79792
                     /locus_tag="PC479_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2802759.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79795..80499
                     /gene="atpB"
                     /locus_tag="PC479_000170"
     CDS             79795..80499
                     /gene="atpB"
                     /locus_tag="PC479_000170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2802760.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            80568..80828
                     /gene="atpE"
                     /locus_tag="PC479_000171"
     CDS             80568..80828
                     /gene="atpE"
                     /locus_tag="PC479_000171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2802761.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80951..81448
                     /gene="atpF"
                     /locus_tag="PC479_000172"
     CDS             80951..81448
                     /gene="atpF"
                     /locus_tag="PC479_000172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2802762.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            81445..81990
                     /locus_tag="PC479_000173"
     CDS             81445..81990
                     /locus_tag="PC479_000173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2802763.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            82007..83509
                     /locus_tag="PC479_000174"
     CDS             82007..83509
                     /locus_tag="PC479_000174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2802764.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            83537..84415
                     /gene="atpG"
                     /locus_tag="PC479_000175"
     CDS             83537..84415
                     /gene="atpG"
                     /locus_tag="PC479_000175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2802765.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            84428..85822
                     /gene="atpD"
                     /locus_tag="PC479_000176"
     CDS             84428..85822
                     /gene="atpD"
                     /locus_tag="PC479_000176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2802766.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85825..86085
                     /gene="atpC"
                     /locus_tag="PC479_000177"
     CDS             85825..86085
                     /gene="atpC"
                     /locus_tag="PC479_000177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2802767.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            86547..86927
                     /locus_tag="PC479_000178"
     CDS             86547..86927
                     /locus_tag="PC479_000178"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2802768.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            86942..87403
                     /locus_tag="PC479_000179"
     CDS             86942..87403
                     /locus_tag="PC479_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2802769.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            87424..88014
                     /locus_tag="PC479_000180"
     CDS             87424..88014
                     /locus_tag="PC479_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802770.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            88029..89186
                     /gene="alr"
                     /locus_tag="PC479_000181"
     CDS             88029..89186
                     /gene="alr"
                     /locus_tag="PC479_000181"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2802771.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            89219..89506
                     /locus_tag="PC479_000182"
     CDS             89219..89506
                     /locus_tag="PC479_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2802772.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            89475..89858
                     /locus_tag="PC479_000183"
     CDS             89475..89858
                     /locus_tag="PC479_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2802773.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            90029..90856
                     /locus_tag="PC479_000184"
     CDS             90029..90856
                     /locus_tag="PC479_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2802774.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            90849..91790
                     /locus_tag="PC479_000185"
     CDS             90849..91790
                     /locus_tag="PC479_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2802775.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
     gene            92213..93307
                     /locus_tag="PC479_000186"
     CDS             92213..93307
                     /locus_tag="PC479_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802776.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            93533..93949
                     /locus_tag="PC479_000187"
     CDS             93533..93949
                     /locus_tag="PC479_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802777.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(94131..94700)
                     /locus_tag="PC479_000188"
     CDS             complement(94131..94700)
                     /locus_tag="PC479_000188"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2802778.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            94854..96011
                     /locus_tag="PC479_000189"
     CDS             94854..96011
                     /locus_tag="PC479_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2802779.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        38729 a        10387 c        17728 g        29650 t
ORIGIN      
        1 tattaaaaac ttaatagaaa tattaagcat aaagattatg tggttacaat agcagagagg
       61 atacacctgt tcccattccg aacacagaag ttaagctctc tagcgctgat ggtacttggt
      121 gggaaactgc ctgggagagt aggacgtagc cacgtaattt tttttatttt ttgtcaaaaa
      181 aaacaaaaaa tacgtatttc ttttttcatt tcgggaaaac taaacacagg aattatggac
      241 tttataaaaa tttaattctt gaggagtgat ataagtgaaa aaagttgctt taattttagc
      301 tataataggt gcgcttaact ggggtgcaat tggtattcta ggtacagact taataggtag
      361 tatctttggt ggaacatatg aaatggtaag tagaataata tacttcattg taggtttagc
      421 tggattatac ttaataccaa gtttaatgtc tgatgataga gaataattta tgcttttatg
      481 ttaaatacct cattctaaat gtttagaatg aggtatttaa ttttataaaa atattaattt
      541 gtttcttttt taatggtaaa atagataaga attgaagttt taaaaagaag tattatgtaa
      601 aaaaggagta ctattaatat atgtttatta ttaatgaatt aaataagatt gtaaatgaag
      661 aagtaatatc ttttcatatg cctggtcata aaaagggaca ggtttataaa aagttaggat
      721 ataaagatat actaaaaaat ttgtataaaa tggatacaac tgagatatta ggaacagata
      781 atttacactt tccggaagga attataaaaa attcacaaaa aaaagcatca agagtattta
      841 aaagtgatta tacttgctat ttagtaaatg gtagtagctg tggaatacaa tctgcaataa
      901 tgtcagtatg tagtcctaaa agtaaaataa taacaaatag agactgtcat cagtctgtta
      961 taaatggatg tattttagga gatatagata tagagtatgt gccttgtgag ataagtgaag
     1021 agacaaatgt cttaaaaggg atatctataa gtaatgtgat tgatataata gataaaaact
     1081 tggatgcaaa agcagtactg cttacttatc ctacatatta tggaatgaca tatgatttag
     1141 aatacatatg taattatgca catagcaaaa agatggtagt gatagtagat gaagcacatg
     1201 gtgcgcattt agggcttagc gagaggcttc ctaaaacagc tcttgagcaa ggcgcagata
     1261 tagttgtaca gagtacacat aaaactcttc cagcatttac acaatcgtca atgatacata
     1321 tacagggaga ccgagttgat atagataaaa tattgaacat gttaagaatt acagaatctt
     1381 caagcccttc atatttatta ttatcttctc tggaattagc agtagatata tatgaaaata
     1441 aaggaaaaga attaatggaa gagcttttaa ataatattat aactttcaaa aataaagttg
     1501 attcaaatat taatatatac gacacaaatg ataaaacaaa aatatttata tcatctaaaa
     1561 atatgggttt aacaggttat gaactagaaa atattttaag aaacaaatat aacatacaag
     1621 tggagttatc taattattat ggagttcttt taatatgtac aataggaaat actgttgagg
     1681 atttctctag tttagaaaca gcattaaaca atattgtaaa ggaagacttt aaatttgaaa
     1741 agttagaaaa taaaaaatac cctgttgtta ttccaaagaa aatattaaca cctagagaag
     1801 cattttataa aactaaaaaa agtgttaaaa tatatgacag cataggtaaa gtttgtggtg
     1861 aatgtatagt tccataccca cctggaataa gtgtaatatc tcctggtgaa gtaataagta
     1921 aagaaataat agactatcta atgttttgtc attctaaagg catgattgtg agtggattga
     1981 aagatgtaga tttaaatttt atacaaataa tagatttaga agacatttaa aaattatgat
     2041 ataaaagtag atagaagtag atagtttaaa attgtgaaga gaaataaata gactgcttat
     2101 atagagctat ctaataaaag tactaaaaaa gtaaaaagtg tttgtatgga ggatatgaaa
     2161 atgaaaggta agttaataat tatagagagt ggttcagatg caagtggaaa ggctactcag
     2221 acagaaatat tatatgaaag attaaaaaaa gaaggctata tgattaaaaa ggtagaatat
     2281 ccaaattatg aatctccatc atctgccttg gtgaaaatgt acctaaaagg agactttggt
     2341 aaaaatgcat cagatgtaga cccatatata gcatcaactt tttttacagc tgataggtat
     2401 gcatcattta aaaccgattg ggaggagttt tacaatgaag gtggaataat tatcgcagat
     2461 agatatacaa cctctaatat ggtacatcag gcctctaaaa tggatatttg tgatagagaa
     2521 gagtatttaa attggttagc tgattatgaa tttaatttat ttaagatacc acaacctgat
     2581 tgtgttatat ttttagatgt gcctattgaa tttagtaaaa agcttatgga gaatagaaaa
     2641 aataaaatta caggggaaga aaaaaaggat attcatgaaa gtgacataga gtatttagag
     2701 aaatcttaca acaatgcttt atatatagca gataaatatg attggaaaaa aataaattgt
     2761 gttttagata acaaattaag aagtattgaa agtatacata atgaaattta taaaatagtt
     2821 cttgattcaa taaaacctat ggagagataa atatgtattt tgaaaatatt atagggcaag
     2881 attttgcaaa gaaatattta acaaattcta taaagaaaaa taaacttaat aatgcgtata
     2941 tgtttgaagg aatggatgga ataggaaaaa agaaatttgc agatgaatta tcaaaactat
     3001 tacttgatta tgaaaatctc gaaaatagtc cagactatgt actcataaaa ccagatggaa
     3061 atagtataaa aatagctcaa atcagaaatt tacaatcaga tatagtaata agacctcata
     3121 aagactataa aatatatata atcaataatg ctgaaaaaat gactgtggaa gctcaaaatg
     3181 cacttttaaa gacgctagag gaacctccaa actatgctat aataatctta gtcacaaaca
     3241 acaaggaatc tttattagaa actataaaat ctagatgtga tataataaag ttctcaccta
     3301 taccaattga agacttaaaa cgttatttga taaatacagg tatagaagaa gaaagagctc
     3361 aattattggc tattttttcg agaggaagta ttgagaatgc tctaaatcta tctcagtctt
     3421 ctgagttttc aatgatgaga gaagatatac aacagtatat tcagataatg ttagataaaa
     3481 acatagtgga gattttaaat ataccaaata atatggaaaa atatagaggt aagattatag
     3541 ctttattaga tatgatgata aactattttc gagatatcat cctattaaaa gaaaatgtaa
     3601 acaagaacat gttaataaat gtagataagc tagttttcat tcaaaatatg agtggaaaaa
     3661 ttagttattc tcaattgtcc aagattattg atataataga agatgcaaag agtaaaataa
     3721 aaagcaattg caatttcaat ataagcatac aagttatgtc tttaaatata tacgaggtga
     3781 ttaaatgata aagatagtag gtataaggtt taaaagtgct gggaaaatat attattttga
     3841 ccctgttgat tttaatatag agcaagatat ggatgtagtt gtagaaactg caagaggctt
     3901 ggagtatggg aaagtagtcg ttggtccaaa agatatggat gaaagcgaat tagtttctcc
     3961 tttgaagcca attataagaa tagctacaga agaagataaa aaagtatatc tagaaaataa
     4021 ggaaaaggca aaagaaactt ttgagttgtg ccaacaaaaa ataaaagaac atgagttaac
     4081 aatgttttta atagattgtg agtatacatt tgataggaat aagttgattt tttactttac
     4141 agcagaagga agaatagatt ttagagaatt ggtaaaagac ttagctgcta tatttaaaac
     4201 tagaatagaa cttagacaaa taggtgtcag agatgaagct aaatctatag gaggtctagg
     4261 gccttgtggt agaaaacttt gttgttcatc ttggttaggt gattttcaac cagtttcaat
     4321 aaaaatggca aaagaccaaa gtctatctct aaatccaaca aaaatatctg gaatatgtgg
     4381 gagattattc tgttgcttaa aatatgagca tgatgtatat agtgaagcaa tagaaaaaat
     4441 gcctgttgta ggtgcattgg tgcaatcaga tgatggaaaa ggaaaagttg tagaagtgaa
     4501 tcctttatta gaacaaatta aagtggaatt tcaagacaag acagtaaaga tatacacaag
     4561 agaagatata aaaatactta gagagcctaa aaaatgtgat ggctgtggtg gtaaatgtaa
     4621 ggataatgat ggattagatg aagccacact aagagagcta aagaaattag aagattaaat
     4681 ttaaggtagg gcttttccta ccttttttaa agcaattaat gcttaaagtt attaatattt
     4741 gaggagaatt tatatatgca attaaaagat tcagaaagaa tagatgattt acaattaaaa
     4801 ggccttaaaa ttatacaaga tactaatggt ttttgttttg gtatagatgc agttttatta
     4861 gccaatttta ctaaaataaa aaaagatgcc aaagtagtag atttgggtac tggaactgga
     4921 attataccta ttttgatagc tggaaaaagt gaagctaaaa aaattattgg cgtggaaata
     4981 caagaagatg tatatgaaat ggcaactcgt tctataaaat taaatggatt agaagaaaga
     5041 gttgagatta taaatgaaga tataaaatac atagacaaag tcttagacat aaatggttac
     5101 catgtagtca catcaaatcc tccatatatg catattgatg gaataaaaaa tccaaatgat
     5161 aaaaaagcaa tatcaagaca tgaagtaagg tgcaatttgg aagatgtaat tagagctgca
     5221 tccagattgg ttatgcctag aggaaaattc ttcatgatac atagaccaac aagattggtt
     5281 gatataataa ctttagggag aaaatataaa ttagaaccaa aggttattca atttgtgcat
     5341 ccaagacctc aaaaagcacc gaacttagtt ttggttcaat ttgtaaaaga tggaaggcca
     5401 gagcttaaaa tattggaccc attgtatgtt tatggagaag atgggaatta tacaaaagaa
     5461 ttaaaagcaa tatataataa taaagatata ggagagttgt aaatgagtgg gaaattgtac
     5521 atatgtccaa caccaatagg aaacctagaa gatataacat atagaacttt aagaacatta
     5581 aaagaagtag acttgattgc agcagaagat acaagacata gtataaaact tttaaatcat
     5641 tttgaaatct caaaaccact aactagttat catgaacata ataaggattc aaagggagat
     5701 tatttgataa ataaactaat tgatggtgaa aatatagctc tgataagtga tgcaggtatg
     5761 cctggtatat ctgacccagg agaagagata ataaaacaag ctatacaaaa taacatagag
     5821 atagaagtat tgccaggtgc aacagccttt gtaacggctt tagttggctc tggtatggac
     5881 acacatagat ttgtttttga gggcttttta gatagagata aaaaagttag gaaagcacaa
     5941 ttggaagaat taaaggaaga aagtagaact ataattttct atgaatcacc acatagattg
     6001 aaggatacac taaaagatat gcttaaaatt cttggtaata ggaatatttc tataaataga
     6061 gaacttacaa aaaaatatca agaaataata agagaaaata tagaaactac cataaaaata
     6121 tttgaggaaa aagaaataag aggagaattt gtattaatag tagagggttt tcatggtgag
     6181 aagtcaaaga aagaaaatta tgatgggctt acagataggg aatatgtttt aaaactaatt
     6241 gaaagtggta tgaacaaaaa agatgcaata aaaatagttt gtaaagatag aaagttaaag
     6301 aaagatgtgg tttataaaca agttttagat ttataaaacc tgaatctgag gtgattttat
     6361 ggaaataggg aaaattataa tagagagttt aaagagggaa gatacacata aaatatttat
     6421 agaattagat aataagcatg aattagaaaa aataattcct aaaataagct gtatgaagtc
     6481 tgtaggtgaa tgtaaatatc atgtagtaaa ttgttttgaa cattctataa acgcattaaa
     6541 agaattagaa atagtattaa atgataagga tttttttcca atacatctaa gaaaacatgt
     6601 gtcaaactat ttaaacacat atattgaaga aggaataaat aagttacatg tattaaagct
     6661 gggaacattt ttacatgata taggtaagcc tgacagtatg accttagatg aaaccggaag
     6721 agttcatttc acaaatcatg aaaaaatagg tgctcaaata attgacaata tgggaataaa
     6781 gttagagtta tcaacagaga catatagact tataagtaaa tatgtaagat atcatatgat
     6841 acttttgtct ctatataaga aaaatgattt gagtaggaaa gaattaatta atgtatttaa
     6901 cttagtagat gaggacacta taggtgtaat aatgcttggg tatgcagata tagtgtctac
     6961 agtaaaatta ttgggcaaga gtggagaagt cagtgtgtta aagacatata tggaatatat
     7021 tttaacaaat tacttgtata agagtaatta tacacatgta taaatttagt taatataatt
     7081 gagttttaga tgattaattg aagtttgaaa tatacggatt tcaaaaacat aaaagaagtt
     7141 gtctttgttg taaaattatg aaagacaact ttttttatat caaaaattga aattttatta
     7201 aggatactta atggtctatt aattaataaa ccattagtat ttaatttata ttacaatatt
     7261 gattttgctt gtattggcgt tgatagtata acagaggtct tagtagaacc aattttcttt
     7321 atagtatcaa tcacattttc taactcgtac atatctttta caattacttt taaaagtaag
     7381 caatcatcac ctgttatatg gtgacattct acaatcctag ggtcctttgc agctgactca
     7441 ataaattctg tatatccatt gcttggaaga gaaatatgaa taaatgcctt tataactctg
     7501 cctaatgaat ctgggttgac aatagcttta tatccttcta taacaccaga ctcttctaat
     7561 cttttgactc tttctgaaac tgcaggagaa gttaaaccaa ctatttttcc taagtccttc
     7621 atagaaattc tcccatcatc ctgtaaaatt tctatgattc tgtaatctgt aacatccata
     7681 aataaacagt cccccttaaa taaaaataat taatgctata aaactttttt agaatctatc
     7741 atttgtgttt ttaaatacca aagatttcta gataaatcaa ccttataaat gtgtggtttt
     7801 gaaagtcttt tgtattgttc ccagatagta cttaattcat attgtacatg attttttatt
     7861 tcaagaacag cttttttatt atatttgcat ctgcctttta tatacaatgg tttaagaagc
     7921 tctttaactt tgtaatttgt aaataccttc gttttccatg tataagtagg atggaagatt
     7981 tctaaaggtt tagactcatc tataacttca tcatgtaaca taatcaaatc tgcctctgcc
     8041 ttattgtctt cgttatatat tcttataact ttcttgtaac caggattatt tattttttct
     8101 ggattctcag aaatctttat cttaggttca agaactcctt tttcacaaga tgcagctaat
     8161 ttataaactc ctccaagaga aggtgaatcg aaagatgtaa tgagctttgt acctactccc
     8221 catgaattta tagtagctcc ttctgcttta agtgatgtta tggtatattc gtctaggtca
     8281 ttagatgcag tgatgctgat atctgaaaag ccagcattat ctaattgttt tttagcttct
     8341 ttagataaat aagctaaatc tccagagtca agtcttatac ccataggttt gtatcctttt
     8401 tcagaaatat ttttgaatac ttttattgca ttaggaactc cactgtttaa tacatcataa
     8461 gtatctacaa gtagtaggca tttatctgga taaacatcag catatgcttg gaatgcttca
     8521 agttcattat caaatttttg tacccaactg tgagcttgtg taccaattat aggtatatca
     8581 aacattttcc ctgctagtac gtttgcagtt ccagcacaac caccaactac agcagcccta
     8641 gccccatata tccctgcatc tggaccttgt gcacgacgta atccaaattc aaatacaggg
     8701 tctccttgag ctgcaaagca aactctagag gctttagtag caataagaga ctgaaaatta
     8761 actattgtta acaatgctgt ttcaataagt tgagcttgat ataaaggtgc cttaacagta
     8821 attaatggct cattagggaa catgatagtt ccttcctcaa cagcatatat atctcctgtg
     8881 aatttaaatt cttttaaaaa ttttaagaac ttgtcagaaa ataaatttaa gttttttaag
     8941 tactctaaat cttcatctga aaaatgaaga ttatctatgt attcaacaac ctgatcaata
     9001 ccacaaacta tagtataccc tccatcacaa gcgttttttc taaaaaacat atcaaataca
     9061 acaatgtctt catgaatatt cttttcaaaa tagccattta acatagtaag ctgatataaa
     9121 tcagtaagaa gtgttagatt tctcattatt tctctcctta attattatta aatattaaaa
     9181 attgtaaaaa ctcccacact tatactaaaa cataatatag tacaactttc aagtataaag
     9241 tattacctaa ctatcataaa attatttaaa gaggaggatg attataatag gaaaaatatg
     9301 gttttatatg gtatcgatag gaataatagg aagcttgatt tctaaaaata tgggtgaatt
     9361 aaataaagtt atattaagtg aaacatcaaa aggtatagag tttgcaataa gtctagctgg
     9421 tatcatggca ctctggatgg ggataatgaa tatcgcaaaa gaatcaggtt taattgaaaa
     9481 gataggtcaa aaactaaatc ctataatgag aagattattt ccttctattc caaaaggtca
     9541 taaagcaatg tcttatatag ttatgaatat tgctttaaat atgttgggtg ctggaaatgg
     9601 tgcaacagca tttggtttaa aagcaatgag tgagcttcaa actttgaata acaagaaaga
     9661 tacagccact aatgatatga ttatgtttat ggtaatcaat atatcatcta tacaaataat
     9721 tccatttacc ataatcaaac taaggatgga tatgggggct caaaatccaa gtgaaataat
     9781 ttttacgact ttatttgcaa ccataatatc tacagcagta gcaataataa catgtaaatt
     9841 ttttcaaaga agatatagat aaaggggatg tagatacatg gagatatttt caaacttact
     9901 tataccaata ataattctgt acatagtaat ttatggtaaa tataaaaaaa tagatgtgta
     9961 tgatagtttt gtaaaaggag caattgatgg attaaaagct gcatgggata tcctaccata
    10021 tataataggt atatttttgg caataggaat attcaaaact ggtaaaggtc ttgatatgtt
    10081 agaatggcta tttacaccaa tagcaaatat gatgagtata ccaaaagaat taataggtct
    10141 aataagtgta aaacctttat ctggaagtgg tgctttaggg atgtacagtg aacttgcaaa
    10201 tagagttgga ataggaagtt tagtagaaaa aatgggtgct acgattgtag gttcttctga
    10261 gactattttt tacacaatgg caatatacta tggaagtctg aagataaaaa atactagaca
    10321 tacattatca tgtgctatga ttagccatgt tgcaggtgtc atagcagctg tttttatttg
    10381 ctatgtaata tttgtataat ttgttgacaa tatgggaaaa attttataat atagtgatta
    10441 tatagaaaca aatagtaata caaattacaa gctatgaaaa gaagagtaaa taaaggtttt
    10501 ttctatcaga gagctaggtt aggtgaaagc tagtgtcaaa agctttattg aagatggtct
    10561 tggagctttt tatctgagca agtgtgctag ggtaaaacgg ttggacacgt tatagtctac
    10621 taaggtatct ataaaatgtt tctacatttc atgtgaaaca taactattat agatgaatta
    10681 gggtggtacc gtgaataatc tcgcccctat gttaactcat aggaggcgat tttttattat
    10741 ttaaatgaaa gaatcatatg attacgaacg ataaaaaatt aggaggtaaa taacatgagc
    10801 aaaccgagtt tttatgtaac aacaccaata tactatccaa gtggaggatt acacatagga
    10861 catacttatt caacagtagc tgcagataca atagctagat tcaagcgttt ctgtggatat
    10921 gatgtaaagt tcttaacagg aacagatgag catggtgaaa aaattcaaaa gaaagccata
    10981 gaacaaggaa tgtcagagat agagtatctt gatggaatga taaaagatat aaaggcctta
    11041 tggaatacta tggatatatc ttatgatgat tttataagaa ctacagaaaa aagacataca
    11101 gacataatac aaaagatatt tactaaacta tatgagcaag gtgatatata taaaggtgaa
    11161 tatgaaggta gatactgtac tccttgtgag agtttctgga cagaatctca attactagaa
    11221 ggaaataaat gccctgactg tggaagagaa acatatttag taaaagaaga atcttatttc
    11281 tttagattat ctaaatatga agacagatta aaagagttat ttaaagatga tagtttctgt
    11341 tttcctgctg ctagaaaaaa tgaaatggtg gcaaactttt tagacaaagg attagaagat
    11401 ttaagcgtaa ctagaacgac ttttgactgg ggtataaaag ttccttttga tgaaaaacat
    11461 gtaatttatg tgtgggtaga tgctttatgt aactatataa cagcattagg ttatatgact
    11521 gataatgatg aagaatttaa aaaatattgg cctgccaatg ttcaaatagt aggtaaagaa
    11581 atagtgagat tccatacaat aatatggcca gcgcttttaa tggctttagg tctagaagtt
    11641 ccaaaacaag tatttggaca tggatggata ctatttgctg atgataaaat gagtaaatca
    11701 aagggtaatg tggtttatcc agaacctata atagaaagat atggaataga cacacttaag
    11761 tacttcttat taagagagtt tgcatttggg caagatggaa gttatactca tagaaatttt
    11821 gtaacgagaa taaattatga tttagcaaat gatttaggaa acttaataag tagaactgta
    11881 gctatggttg aaaaatacaa taatggtata ataccaacag caaaagtttc aactgatttt
    11941 gatgctgact taaaagaaca agctgtttca actagagaaa actttgaagc tgaaatggat
    12001 aaaatgcaat tccatgaagc attagagagt gtatggaaat tagttagaag aactaacaaa
    12061 tatatagatg aaactatgcc ttgggcacta gctaaagatg aaacgaagaa agatgaatta
    12121 gacacagttt tatataattt atgtgaatct ataagaataa tcgcaacact aataaatcct
    12181 ataatgaatg agactgctaa taaaatatat gaacatatag gaataaaagg tcaagatgat
    12241 ataactacat gggaaagtac aaaaacattt ggtcttattg gagaaaatgt aaaagtattt
    12301 aaaggtgaac ctttattccc aagattagat gtagaaaaag agatagaaga attaactaaa
    12361 atgttctctg ggaaacctcc agtagaagaa aaacctttag agcataaaga agaaattact
    12421 attgatgatt tagacaaaat agagcttaga gtagggaaaa taataagctg tgaaaaacat
    12481 ccaaaagcaa ataaattatt agtatcacaa gttaagattg gaccagaaac aagacaaata
    12541 gtatcaggaa tagctgaata ctataagcca gaggatttag taggaaaaga ggttacagtg
    12601 gtatgtaact taaaaccagt taaattaaga ggtgtagaat ctcaaggtat gatattagca
    12661 gcaggtgatg atggagaccc atatgtttta ccatttacac aaggggctaa agatggatgc
    12721 gaggttcgtt agtaggtaat taaatagtaa ggagattaaa gatgttattt gactcacatg
    12781 cacatttaaa tgatgaaagt tttgatgaag atagagatga acttattggt tcattaaaag
    12841 ataaaggtgt agatttggtt gtaaatccag gagcagatat agagacgtcg ataacagcaa
    12901 ttgaacttgc gaaaaaatac gactttatat attcagcagt tggggtacat ccacatgatg
    12961 tatcaaaact agatgataca gcaatagaaa ctctaagaaa acttgcaact gagaatgaaa
    13021 aagttgtagc tattggagag attggattag attattatta tgattattct ccaagagaag
    13081 aacaaaaaga gtggtttaag aagcaaattg aattagctaa tgagctaaaa cttccaataa
    13141 taatacatga tagagatgca catggagata cttttgagat aataaagaat actaaaaatc
    13201 ctgaaatagg atgtgtactt cattgttata gtgggaatgt agagttagct agagaatatg
    13261 ttaagatggg ttgttatata tcaattccag gcacggttac ttttaagaat aataaaaaaa
    13321 ctagagaagt agtaagagaa atacctctag aaagattatt catagagact gattcaccat
    13381 atatgtcacc agagccacat agaggaaaga gaaacaatcc ttcccaagta tcttttgttg
    13441 ctgataagat agcccaggaa aaagggatat cttatgaaga agtatgtaga gttacaaaag
    13501 aaaatgcaaa gaaatttttt aatataaaat agacaaatta aatcttataa tgatatatat
    13561 aataagtcat tataagtata ggatgagagt gcctcaaaat gagtttttta gttcatgagg
    13621 cactcttttt tgtatgaaat caaatatatt ttcatcattt caacaatgaa aaagaagttt
    13681 atcatatttt gagatagttt gtttttattt atttaattca tattttaaaa tatattttct
    13741 taacattgtt aaaaaagaat ttacaagaga ttcattagaa ttaactcaac tctaatattc
    13801 acttaactta agttgtttat tatattatta aagagaagta aacgaaaggt agattaagga
    13861 ggttgaattt tgtatacagt tgatagtact aagatattga taaatatgaa cttagagaaa
    13921 ctagaaaaga tatactcaat gataaaagat acatcaagta tttctataat taaagaacct
    13981 aacttggcta cagtaatggt aagagctaat gaaagtgtaa aaaacacaac atttaattta
    14041 ggagagatat tagttactga atgtagtgta aaggtagatg aaagcttggg ttacggaata
    14101 gtttcagaaa acaataataa gaaagccatt tatttagcag ttatagatgc agttttacat
    14161 agcaataaca gtaaatttga tgagttaaag aattacataa ataaaagtgt atatgaagag
    14221 aacttaaggt atgaacaaga gattattgat gaatttagct tgattaataa aacgaaagtt
    14281 caattcaatg ctatggatta aataggagga gaatcatggt aaattataca caagaaatat
    14341 atagaaagat gctagatagt gcttcatttc ctgggaaatt aaataatata gataatataa
    14401 atatagaaaa taatacaaat ctattgaatg gagcaattgg tatagcaatc actttattag
    14461 accaagaagt taccttctat atagaaaatt atactcaaaa agatataaaa aatataagag
    14521 cacttacaat gtcaaatcaa atagattata gaaaatcaga ttatatattt ttagatatta
    14581 attcagaagt ggatatatta cagattaaaa taggttcttt tgaatatcct gatgaaagtg
    14641 caactatagt acatcaagta aatgatttat caacgtactc tcaaactaag tatataaagc
    14701 ttgatttgag tggtcctggt ataaaatcta aaaatagtat atatataaat ggaattagag
    14761 aagaatttat agaaagatta tctagtataa ataaagatta tccaatagga atagatttga
    14821 ttttagtaga taaaaaagga gaaattgcat ttataccaag gtcatcaaaa ttaagttggg
    14881 aggtattata gaaatggctt atgtagcagt taaaggtgga gaagaagcca tattacaggc
    14941 taaaaatata cttgagtttt atagggtaaa aggagatagc ttgcctatag atgtaaagca
    15001 gattgaggaa caaatgtctt atgctgtaga caaagtaatg tctgaaggaa gtttatacaa
    15061 caaggaacta tcagcattgg ctataaaaca agctcaaggt gatatattag aagcctcttt
    15121 ctatgtaaga gctttaaggt caacaatgcc tagaattaca tattcagaac ccataaatac
    15181 agaagaaatg agaataaaaa gaagaatatc agctactttt aaagatatac caggaggtca
    15241 gtatcttggg gcaactagag actatgaaaa tagaatattg aatgtagaat tattagaaga
    15301 agatgaaaaa aagaatagac attttagaga tagttttata aatgaattag aaacagtaga
    15361 tgaaatccct gaatttacaa aaatatctaa tatatttaga aaagaaaatt tattacaaga
    15421 agaagtttgt gaaaacaaat tggaagaagt tattgatgat attacaagaa atagtttaag
    15481 atttccagtt tctagggcag caaagttaca ggcattaact agaggtgaaa gcggagtagt
    15541 atcaggtttt gcttatgcta ttgtaagagg ctttggtgat gaacatccat atataagtga
    15601 acttagaact ggcaatgtgt ttataaaaat aaaacatccc atctatgaag atgaagaaat
    15661 aacaataggt gatatgttgg ttactgaatg tgaaatagtt tcaaaagcat cgcaaaataa
    15721 gactgaaaat tctaaaaata attcggaaga taaaaataaa atgtctttag gatatggtct
    15781 ttgtatgggg aacaatgaga ataaggctat atctatggct atactagaca aatctttgga
    15841 gccaaatagt gaaaaactag cacaagacga agaatttgta ttgcttcata tagatggaat
    15901 agattcatta ggatttatat ctcactttaa gcttcctcac tatgtagatt ttaaagcgga
    15961 tgtagaaaga ataaaaaagt ttgaggaggt aagtttaggt gatatataac tatggttttt
    16021 tagacgaaaa cacaaaaaaa gaaataagaa gaaagatatt aaaagcaata tcaatacctg
    16081 gttatcaagt tccctttggt tcaagagagt tgcctatagc taagggatgg ggtactggag
    16141 gtttacaatt aacactatca ttaattggta aaaatgatgt agtaaaagta attgaccaag
    16201 gaagtgatga ttctacaaat gcttgtaata taaggagctt tattgcaagt gtaagtaatg
    16261 tagaaaccac aaaagataca ttagaagcta cattgattca aacgagacat agaataccag
    16321 aagaaaaact taaatctaat caaatactaa tatttcaagt accaatacca gaaaccctta
    16381 gaatagtaga acctagtgaa gtagaaacaa gaagaatgca ttcagaagaa gattatagta
    16441 ggatgtgggt atatctttat gaagatatag ttagatttaa tgatatatct atagcagttg
    16501 aatatccatg taaagtaaat gatagatatt taatgaatcc atcacctata cctagatttg
    16561 atataaaaaa attaaatatg tcagacaatt tattcttgtt tggtgcaggt agagaaaaaa
    16621 gaatttatgc tattcctcca tatacaaagg tagaaccttt agagtttgag gattataagt
    16681 ttgaagagga aaagtttgaa ggaaaatact gtagtttatg taacagtaca aatacatttt
    16741 tagatgaagt atatgacagt gacactaatg aaaagtacta tagttgctca gatacaagtt
    16801 attgtgaaaa agttagatta aaaaataaca gtatagatgt gacaatagga ggtacttgga
    16861 atgagtaaaa taaatgactc taaaagatta gaaaatatag acaagcaagt attaatagga
    16921 agaaatatca atcttattta tggagatggg tgtgaaaagt gttttgaaag cacaggatat
    16981 gaaagcaata atatatgtaa gtattgtggt tctatagttg catgtaataa tgtaaattta
    17041 gaactttatg aaggagaagt ccttggaatt gtaggtgaat ctggttcagg aaaaagcact
    17101 ttattaaaaa tactgttttt ccaagaacat gcaaatagtg gagaagtata tatatcccat
    17161 tacaataaag acacaaatat actagagctt tcagaccaaa aaaagagata cataaagaat
    17221 cattttatgg gtattgtgta tcaaaatccc caccttggtt taaatttaga ctttagtagt
    17281 ggtggtaata tagcagaaaa attattgatg gcaaatttat ataatgttga aaaaataaga
    17341 gatagaggca aagaactttt aataaaaact aatatacctg tagaaagaat agaccataaa
    17401 ccaaaatact ttagtggggg aatgcaacaa cgagttcaaa tagcaaaagc tctttcaaat
    17461 aaccctccaa tattgctatt agatgaggtt acaacaggtt tagatgtttc tgtgcaagcg
    17521 gaagtattgg atttaataag agaaatccaa agagaattta agatttctat gatagttgta
    17581 tcccatgact ttgatgttat aaagatgctt gctgatagga caattgtaat gaaaaatgga
    17641 agtgtaattg agtctggtct tacagaccaa ataatggagg acccacaaca cccatatact
    17701 cagcaactta taaattcatt gctataaata tcgtttgtaa ataagcaaaa tgggggtttt
    17761 taataaaatg aaagaaacta tacttgaagt aaataatttt agtaaagact ttgagttaca
    17821 tgcattgaac aagactataa cggcatgtag taatataagt tttgaagttt caaaaggtga
    17881 gtttttagga ataataggta aatctggagc aggtaagtct accatattaa aaagtatcta
    17941 taaaacatat ataccaacaa ctggagaaat aatttttaat tcagaaatat atggtaatgt
    18001 agacctggct aaaattggag accgagaaat gataaatctt agaaagaaag aaataggata
    18061 tgtatcacag tttttaaaga cacttccacg aataactgca attgaactgg tagtacattc
    18121 tttaattgag agtgggtttg aaaaggattg ttcgtatgat atggcaaaag atatacttaa
    18181 ccaatttgaa ataaaggaaa atttatggga tgcgtatcca aataattttt ctggtggaga
    18241 aaaattaaga ttaaatttag ctcaagctat ggttaaaaag cctagacttt tgttacttga
    18301 tgaaccaact gcatcacttg ataatcaatc taaaatatat gtaaaagaaa aaatgttgga
    18361 gcttaaaagt caaggaacta caatgatagg aatttttcat gatatagagt ttatggaaac
    18421 tgttatagat aagaccttta ctatgacaaa agggactata agtgaaagtg gggtagccta
    18481 gatgataaaa ctaattcata atgcaaatat tgtattagaa gatatgattt tagaaaatgg
    18541 tcatttaatc atagagggag atacaataaa aaaagtatca aatgataaga ttgattacgc
    18601 atttgacttt aatgagatag atgaaataat agatgcaaaa aacttatatg taatacctgg
    18661 tattatagat attcacagtg atgccattga aaaggaaata gagccgagac caagtacatt
    18721 attaccattt aatatggcat tttatgaact agataaaaaa ctacctgcaa atggaataac
    18781 tacagtttat cattctatat ctttagggga tggagtagga gttagaagta ttgataactc
    18841 tttaaagatg attaaaaata ttgattcata taaaaatata gatagtaaga gcataaatca
    18901 taaagttcat ttaagatatg aagtgttgta ttatgaaggt ttagaaaagg ttttagaact
    18961 attagatgaa aataaaatag actacttatc aataatggac cattctccag gacaaggtca
    19021 gtatacaaat ccaacatttt ataaagagta tgctactaaa gtgtggggag ttacagaaaa
    19081 ttatgtagat acatggttag atgatttagt aaatctgcat gataatttag attggaataa
    19141 aatagctaat attataggta tagccaaaac taaaaatata aatgttgcat cacatgatga
    19201 tgatacatta gaaaaaatgg atttcataaa aaatataggc ataaatgtat ctgaatttcc
    19261 tataactctt gaagttgcaa aacattcaaa gaaattgggt gttctaactt gtttaggtgc
    19321 accgaatatt gtaagaggta aatctcataa taacaattta aaagcaatgg atgctatatt
    19381 ggaagattgt tgtgacattg tgtgttcgga ttatcttcca tcagctatga taaagtctat
    19441 gtgtatagtt gcagaaagaa taaataattt aaataaagca gtcagtttat ttacctcaaa
    19501 tcctgctaaa gcagttggta tatatgatga aagaggtagt ataaaagaaa ataaaaaagc
    19561 agatttagtt ttagtagata ttgatagtga atatccaaag gttgtaaata ctattgtaaa
    19621 tggaaaaaca gtttataaaa gggaggtata aagattgcta acagagttac attgtcatac
    19681 aagtatatca gataataatt ttactactga agaaataata aaaaaagcag cagaaaagaa
    19741 tataaatatc ttagcaataa caaatcatga cacattatct ggattggatg aagctcttgt
    19801 gcttggagag cattatggaa taacaataat accaggtata gaaatttcag cctatgatta
    19861 taaaaataga aaaagagttc atattcttgg atataacata gatttaaaca gtcaagaaat
    19921 aaaaaatttg tgcaatccaa tggtatgtga cagacataaa gcatctgtta agatggtaaa
    19981 taaaattata gatttaggat acaaaatatc tttggaagat gtaaagaaat attcatcaaa
    20041 gactggaata tttaaacaac atataatgaa agctcttata gataaaggat atacagataa
    20101 tatatattca agtctatata aagaattgtt tcatagagga aacggcaagg tatatgtatc
    20161 tttaaaatat gtggattaca gagatgcaat aaaagctata aaaaattctg gtggaatttg
    20221 tgtgttagct cacccgggtc aaatggataa ttttagcgct attgaagaga tggtaaaagt
    20281 aggattagat ggaatagaag tatatcatcc atctcacaac aaagaactag aaaaagagtc
    20341 gctttcttat gctaagaagt ataatctagt ggtcacagga ggctcagatt accacggatt
    20401 ttattctcaa cataattatg aacttggaag caagagctta aataaagaag atttatttaa
    20461 gtttaggtta gctttaggag gggtattata aatgtctttt ttacaagtaa aaaatgtagg
    20521 taaaagttat ggacaagtta aggtgttaaa agacatatct attgatattg aaaaaggaga
    20581 gtttatttgt ttactaggtc caagtggttg tggaaaaagt acactgctta gaataattgc
    20641 agggcttgag gataaacatg gtggaaaaat aataataaat gataaggata tgactaattc
    20701 accacctgaa agtagaaatt ttggtatagt ttttcaatca tatgcattat ttccaaatat
    20761 gaatgtttac aaaaacatag cctttggact agaaaataaa aacataagta aatctaatat
    20821 tgataaaaaa gttaaagaag ttctagaagt tgtagaatta agtggatatg agaaaaaata
    20881 tccatcacaa ttatcaggtg gacaacaaca aagggttgca ttagctcgtg cgattgcttt
    20941 agagccagac tttttacttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact
    21001 taaacttagg gagcagatta gaagtttgca tagaaaacta ggcattacaa ctataatggt
    21061 aacgcatgac caagaagaag ctctttgttt agctgataag atggttgtta tgaatagagg
    21121 agaaataata caagttggaa cgccaaagga ggtatataaa aatccagaaa caccttttgt
    21181 agctgatttt ataggaacaa taaattttat agatgatggt attaataaga tagctattag
    21241 accagaagat ataaaagtag agtctaatag agattctaaa gataaggata taaaagtggg
    21301 agaaatttta gatatcgaat ttagaggatt taactataga attacagttg agtatcgttc
    21361 taagcagatg aaattagatg tagtttccaa agttgcagaa caaatgaaac tgtgtatagg
    21421 ttctaaaata aactttaaga tacctaaaga aggaattgta caatataaat cagagggatg
    21481 tgcttaatag ctaggggtga ataaatgata acatcaaaaa ataaaagaag aataagtgat
    21541 agcacaatac aaaaatattt cttgattttt tcggtaattg tactaatagt atttatatta
    21601 tttccattag tatcattatt aaaaaatgca tttatggata caagtggaaa ttttatagga
    21661 atttcaaact ttagtaaata tgttgaaaat ccaagtctta tagcatcatt taaaaattca
    21721 atttttgtat ctacagtctc atctttaata tcactatgtt tagcatttat atattcatac
    21781 gcaataacaa gaacaaacat aaagtttaaa gaatttttta aaactatggg aatgtttcca
    21841 ctttttgcac caactatgtt atatggtatt tcacttatat acctttttgg aaataaggga
    21901 atctttacaa ccatgggtct ggaagtacca ctatatggtc cattaggaat aattatttct
    21961 cagattctat ttacatttcc acaagctttt ttaatattat ctgtagcact ttcgatggct
    22021 gattacagac tttatgaagc agcagatagc ttgggtgcta gtgaatttaa gaaattttgc
    22081 aaaataacaa ttcctggtat aaagtactca ttacttagtt cattctttgt atcctttata
    22141 cttgcattta cagattttgg agcagctaag gtagttggag gaaactacaa tgtactggct
    22201 actgacatat ataaacaagt agttggacaa tttaacatac caatgggagc tactgtttct
    22261 atggtaatgt taattcctgt actaatagca tttacattag ataagatggc aagtaagaaa
    22321 caaggtatga ccataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga
    22381 gatagagtat ttttaatagt atgtagtttt ataacattgc taatttttat tttaatattc
    22441 gtttcagtta taggttcagt tataaagtta tggccatata atttaagctt tacaatggaa
    22501 cactatacat ttaaaggtat tttgggaagt ggaataaaaa catatttaag cagtttgaaa
    22561 atatcaactt taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata
    22621 gaaaaggtgg ataagtttcc taaattaaga caaacagctt accttttatc aatgatacca
    22681 atggccttac ctggcttagt tttaggtata tcatatataa ctttttttaa ctcagcaagt
    22741 aatccattaa attttttata tggaagtaca gcaatattag ttatagtaaa tgtagttcat
    22801 ttttattcag tttgttttat aacctcaaat tcatctttaa aaatgttgga taaagagtat
    22861 gaattagttg caaaatccat aaatattcca ttctataaag tattttttaa tataacagtt
    22921 ccaatgtcta taacatcaat acttgaaata gtagtatatt attttgtaaa ttcaatggtt
    22981 actgtttcag cattaatatt tttatataca cctcaaactc agactgcatc aatatcaata
    23041 ctaaagttag atgagatagg atatatagga ccatcagctg ctatggcagt attagtgctt
    23101 ttaacaaata taatagtaag acttttgtac gaatttgtaa caaagaaatt aaaaaataac
    23161 acacaaaaat ggcagcaaaa agatgttgca taggggaggt aaaaatgaaa tttaagaagc
    23221 tagtttcttt aggagtatta tcaacacttt tattaagtgt aataacagga tgtagtactg
    23281 attcatcagg ttcagattca aagtctgata gtaagacaaa aggggattta acagtttaca
    23341 cagcaattga agaagatagt atagagccat atttagctac atttaaagag aaatatccag
    23401 atattaaatt aaatattgtt agggcatcaa caggagatat aacagcaaga ttattagcag
    23461 aaaaggataa tcctcaagca gatgttgtat ggggagttgc agccacaagc ttattggttg
    23521 cagatgacca ggggatgtta gaaccatatg ctccaaaagg ttctgaagaa atagagtctg
    23581 catttaaaga tgataaagaa gttccttctt gggttgggat agatgcttgg atgacaggta
    23641 taacagtaaa cactaaagaa ttaagtgata agaacatccc agttcctagt tcttacgaag
    23701 atttaataaa gccagaatat aaaggtctta tatctatgcc taatccatct tcttctggta
    23761 cagggtactt aacagtatca gcccttatac aaattatggg ggaagaaaaa gcatggcagt
    23821 atatggataa gttacatgag aatattggtg tatacactca atctggttca gctccagcta
    23881 catcagcagc ttcaggagaa tatcctattg gtatatcttt tggatataga ggtataaagt
    23941 taaaagagga aggctatcca gtagaagtag tatttccaaa agaaggttct ggatgggata
    24001 tagaagcaaa tgctcttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag
    24061 attgggctat aagtaaagat gcaatgaacg aatactctaa aaactatgca gttactacta
    24121 tttctacagg aaatccaata ccagaaggat tccctaaaaa gccattagag caaatgatag
    24181 ataatgattt aaaatcagca gcaaaaaata gagaagatat actaaataaa tggatatcaa
    24241 aatatgatgg taagacagaa aaagagagtt aagtaaaact atacaaatgg agtaaaaaga
    24301 aaaatttttt ttaagtagat tttattatta taatataagt tgagaataat ataggtcaag
    24361 aaaagtgaat attctataaa aatgtataga gtaagtatac cattcagttt gaatggtata
    24421 ttttttattt ttagagatta atttagaaat agatatacta cagtttaaaa tagtatattt
    24481 taatttcatt attaaagtat agatgttata tataaagtaa ataagatatt aaataattat
    24541 aaactaaagt aaataaaata ttgagcgatt attaaaaaga tatatatata atttaatttg
    24601 agcagtttgt tatatattat aaaaaaagag aaaaataaaa ggtaaattgt ttctggataa
    24661 cttaaaattg gaagtaagaa tatttaacta gagaagaatt atttaagttt aggttagctc
    24721 taggagtagc tgattttata ggaacaatag attttatgga tgatgatata aacatggtag
    24781 caattagacc agaagatata agggtagaat ttaatagaga tttcaaagaa attatagacc
    24841 tcaaactcag accgtatcga tataaaaact aaagttagat gatatagtgt tgtagatagg
    24901 accatcagct gttatggcag tattagtgct tttaacaaat ataatagtaa aacttttgta
    24961 tgaattgata ataaaaaaat taaaaaccaa gacataaaaa tggcaacaaa aagaagttga
    25021 ataagggagg taaaaatgaa atttaagaag ttagtctctt tgggaatatt atcaacactt
    25081 ttattgagtg taataactgg atgtagtaat gtttcatcag gttcaaactt aaagtctaat
    25141 aataagacaa aaggagagtt aacagtttat acagcaattg aggaagatag tatagataca
    25201 tacttagcta catttaaaga gaaatatcca gatattaaat tgaatgtact tagattatca
    25261 gtaggaggta taacagcaag attattagca gaaaaagata atcctaaagc agatgtgtta
    25321 tggggtgttg cagctacaag tttgataatt ttagatgaca aagaaatgct agagccatac
    25381 gctccgaaag gctttgataa ggtaaattct gcatttaaag atgataagaa agttccttct
    25441 tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga attaaaagct
    25501 aaaaatatat cagctcctag ttcttatgaa gatttaataa agccagaata taaagatctt
    25561 atatctatgc ctagtccagc ttcttcagga acaggatatt taacagtatc agaactcata
    25621 caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa aaatattggt
    25681 gtatacactc agtctggttc agctccagct atattagcag cttcaggaga atatcccatt
    25741 ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc agtagaagta
    25801 gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt gaaaaaagat
    25861 aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga tgcaatgaat
    25921 gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt gccagaagga
    25981 tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc agcaagaaat
    26041 agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga aaaagagagc
    26101 taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt atatctaggc
    26161 tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag tttagtacct
    26221 tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta aaaacgaata
    26281 aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat gacgttacag
    26341 cagttaagag agcaatagat gcagaactta taactacagg tggatttgga tttccaaaag
    26401 gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata tttacagacc
    26461 cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca ggatgtaaac
    26521 atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt gagaatgcta
    26581 ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca gaaaaaagag
    26641 atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa gaagcatcac
    26701 ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca aaacaatttt
    26761 taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca ttaaaacaaa
    26821 tctaaacgta atgtttaatg tatgaaaaat atgtatataa aataaattgt ggaattacgg
    26881 ttaatttaag atgaattatt tataatatac accttatttt aaatcaatgt aaatacatat
    26941 aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt aaataatgga
    27001 tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt ttaaattttc
    27061 aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa tcttatctgg
    27121 agctagaatc acaagaggag ataatatcat agaagtagga cctggtatag gaactcttac
    27181 tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata gaaatttaat
    27241 tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa atcaagatat
    27301 actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc cagttaagct
    27361 tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt tagaggaaga
    27421 catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata gaatgaatgc
    27481 tattccgggt actaaagatt atggggcatt atctatagct gttcaatact attgtgatac
    27541 agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg tagattccac
    27601 agttataggt ctacatgtaa gagataaaag aaaatatgat gttcataatg aagacatatt
    27661 ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta attcgttggg
    27721 aggtcttggt tttcttaata aagatgaaat aagagaagta ttaaaagaag ctaatataga
    27781 cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt caaatataat
    27841 aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa catatattgt
    27901 ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt taaaagagac
    27961 tatataatac tagaagctaa agacataaac tttagataca aagaacgagt actgccaaaa
    28021 gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata tgtagaaaat
    28081 ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca tggattaata
    28141 gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt agatttagaa
    28201 gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat acctttaatt
    28261 ggatttaaag gaaataagct tgaaaattat gaggacatat tatttcaaga ggtagaggaa
    28321 gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga ggagaatgag
    28381 gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata tgatgaagat
    28441 gagtatgaag actattggga agatgatgaa gatattgaag aaggagagtt aaaagcaaga
    28501 acggaagtag aagattctga tgatgaggaa atagaagagt tcaataatta taaggaaata
    28561 gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata tgatgaagaa
    28621 gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt ttcctcaaaa
    28681 cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat aaaaagagct
    28741 aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact tatgccaaga
    28801 caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac agattatata
    28861 gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg atatacaatg
    28921 ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc agttttacat
    28981 tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa taagagaaaa
    29041 cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg aagcacagga
    29101 tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg gctatgtttt
    29161 atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa aaatttagac
    29221 tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta aaaactattg
    29281 ataatattta aatattacac tacaatatat cttgaaatac ctattatatt atggaggaaa
    29341 attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca gatagatagt
    29401 ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga agaaataggt
    29461 tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg taatataata
    29521 gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca tatggataca
    29581 gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat aataaaaagt
    29641 gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat attagaaggc
    29701 ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt attctctata
    29761 tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat agattctgaa
    29821 tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa agctcctgca
    29881 caagatgtaa taaatgttaa gatattagga aagactgctc atgcaggtct tgaacctgaa
    29941 gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa tttacttaga
    30001 atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc tacaaatata
    30061 gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa taaattagat
    30121 gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt tggagctcaa
    30181 attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga ttctgaaata
    30241 ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac tgaatctaca
    30301 ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat aactttagga
    30361 attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga cttatatgat
    30421 tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat agtgtataat
    30481 atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt gataatctct
    30541 attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg ttcaaatgta
    30601 ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa acaaggtatg
    30661 aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta acaataatta
    30721 aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga ttacattcag
    30781 catcagatat tataagttct ataggagtac taataggcaa ttatgtatct tctagacctg
    30841 gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca tttgtgttat
    30901 caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa tctttattta
    30961 acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt atttcaatcc
    31021 tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata aattcaccag
    31081 ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta gctgctttta
    31141 taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg tcagttgtag
    31201 tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt aatgaattga
    31261 tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta gcagatacag
    31321 aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg gcatatgtag
    31381 atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat attgctacca
    31441 agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca gttcacgttg
    31501 aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt aaaaaaaagt
    31561 gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa atgagcaaga
    31621 aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc tttataatat
    31681 actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa gtgccaaaga
    31741 aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa gagaagaaga
    31801 aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat atgtgggatg
    31861 aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct tcttacgaag
    31921 cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt aactttttta
    31981 gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct aaggatgctt
    32041 ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt cattataaag
    32101 attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat agatggaaat
    32161 ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt caacctgaga
    32221 aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat aattttgatg
    32281 taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa aatgtatctt
    32341 actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt gaaattggaa
    32401 agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa ttattttata
    32461 tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc agcagttatt
    32521 actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc aaattctttt
    32581 atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt agaatgtata
    32641 tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt atcagcagca
    32701 tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt atgtggctgg
    32761 aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt tgcttttaac
    32821 tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta ccctttagtt
    32881 tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt ctctaagtca
    32941 ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt atgaagacca
    33001 tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct aaatattcca
    33061 tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga atctctgccg
    33121 tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc atcatcgcca
    33181 tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa agaagccttg
    33241 atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa aactatagat
    33301 atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt tataaagtgg
    33361 ttggaattac ctatcttaac attaccacct atcatactta agttaccacc aactagtata
    33421 gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt agaagttttt
    33481 ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg gcctaaaaaa
    33541 gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc ttcattgtct
    33601 ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt atcttttttc
    33661 tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta tgattcgttt
    33721 gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa tgcactcatg
    33781 ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt ctttttaatt
    33841 aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat attttataat
    33901 taatatgcat ataatattat atgtatttta tttttgaata taataaaaca taagtaatat
    33961 tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa ggtacttgta
    34021 ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag ttaaaatact
    34081 gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat ttaatgcagc
    34141 aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga agctacattt
    34201 aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac ctaaaacttc
    34261 tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg acaagtctag
    34321 aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta caatagctgc
    34381 taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt atgttgtgac
    34441 aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga atttacctct
    34501 agttataata agaaaagata taaaggtatc agaaggtcct acacttagca tgacatatgt
    34561 gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc ttaaaccaga
    34621 tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa aaggaatggt
    34681 agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca tatctacaac
    34741 gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg atgttaatgg
    34801 agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata gaaatgaaga
    34861 tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt aataaaaaaa
    34921 atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca tagaattatt
    34981 taaaaaacag tttatattaa tttattccag cgataataat tttaatataa attaatttct
    35041 gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt aagaaaatta
    35101 acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt gtttgtagtg
    35161 catgatataa aagttataga aggccataat gggctattta tagcaatgcc tagcagaaaa
    35221 gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat gagacaagtt
    35281 ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga agtagctgct
    35341 gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt tttaaatgta
    35401 gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta gtgataatgt
    35461 atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt aagagaataa
    35521 tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa gctataatcc
    35581 ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata cataaagtat
    35641 gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg gtaaaagata
    35701 ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca gaagaaatta
    35761 taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg gctaaagaat
    35821 atattaatga tgaagataca atagttgtac tttgtggaga tactccactt ataaaagaag
    35881 agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct acagttctta
    35941 caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag aagggtgacc
    36001 ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata agtgagataa
    36061 actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat ttgctaaata
    36121 ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg agagataaag
    36181 gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc gttaattcaa
    36241 gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa tctcatatgg
    36301 taaatggagt aactataata gacactaact ctacctatat agaatctgat gttatgatag
    36361 gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg ataggttcag
    36421 attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat ggtacagaaa
    36481 taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt gggccatatg
    36541 catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat tttgtagaag
    36601 taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat ataggagatg
    36661 cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac tatgatggta
    36721 aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat tctaatttag
    36781 ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca ataacacatg
    36841 atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa gaaggatggg
    36901 tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta ttattttggc
    36961 taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa aattttcagg
    37021 agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg taattcatcg
    37081 aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga ttgtgaagta
    37141 ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag aggttgtgat
    37201 gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga gttattaatc
    37261 ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat tccttactat
    37321 ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc taaattagtt
    37381 gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt acatgcagct
    37441 caaatacaag gatattttga tataccatta gaccacttac taggtggaac aatattagca
    37501 aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga cttaggaagt
    37561 gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat agctataata
    37621 gataaaagaa gaccgaaagc taatgtatgt gaagttatga acttaatagg tgatgttaaa
    37681 ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat agttaatgct
    37741 gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac tcatggtgtg
    37801 ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt aatagttctt
    37861 gatactatac aacttcctga agagaaaaga atagataaga taaagataaa aacagtagct
    37921 cctttatttg gagatgctat aagaatgata ttctctaatg aatcagtaag taaattattc
    37981 taatttattg tatttatgag agttgtttaa aaggatataa accttttgag caactctttt
    38041 ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat attattgtaa
    38101 aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat aaagggtata
    38161 ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattgaaaa aattcagatt
    38221 atgtagtatt atgtaaatat aaataaagaa agaggtgttt ccaatgggaa ggttagaaga
    38281 tgaaccccta ataattaaat gattcagtca caatctaaag tgaatataaa aatctattga
    38341 aagtttattt ataagaattg aaaatagtat ctattctatc tttagtgttt gtgagagggt
    38401 tctggattat ataagattac tgtaaagagt aattttgcac aataagcata tctgtaaatg
    38461 aagagtttcg tttatataat gattgtggct gaatcataac tataatagat agaatgttta
    38521 taatagatga ttttataaaa tactatattg taatagcttt tagctttcaa ataaatatta
    38581 gcaaaggatg gaaatgacaa tgataaaact aaatgataat ttataaatat ctccttacga
    38641 tatataataa ttgtagggag atgtttcttt ttttgtatag cataattttt ctagaaattc
    38701 tgtttcaata tgtaaaaagt tggtatatat aaatagtata aacagtgttg ctaaaatatg
    38761 gtagatatta tataatataa gtggatatgt atatacatta tgagttatat aatttgaatt
    38821 taataaaata gaaacgttga tttatgttct gtaatgtggg caccttggag catattgagt
    38881 tagtggtgca accggctatg aaattgtatt tatttataaa tactattatt ttcatagctt
    38941 tttttattta aaaaatggtt atatgttaga aataaatttg gttagtacgt ttaaataatt
    39001 ttacttagat ttttaaccag atatattgta agtaagtatt aattaaaaaa tttaaggggg
    39061 aataaaaatg aagttaaaaa agaataaaaa aggtttcact ttagtggaat tattggtagt
    39121 aattgcaatt ataggtatat tagcagtagt ggcagttcca gctttattta gtaatataaa
    39181 caaggctaag gtagcaagtg ttgagtctga ttatagttca gttaagagtg cagcattatc
    39241 ttattattca gatactaata agataccagt tacaccagat ggtcaaactg gtttaagtgt
    39301 tttagagact tatatggagt ctctgcctga taaagctgat ataggtggaa aatataaatt
    39361 gattaaagtt ggtaataaat tggtattaca gataggtaca aatactgaag gagttacatt
    39421 aacagaggca caatcagcaa agttattgag tgatataggt gaaaataaaa tatatacaag
    39481 cgctacaaca aatagtttgg gaaatccatt aacaagtaat acaaaaatag ataataacgt
    39541 tctatatata gtacttatag ataatactgt gatggacaca acaaaatagt taaaacaata
    39601 tttttataaa aatattgtaa atttaatgaa aaatataata taaaagacag gaagaaaatc
    39661 ttcctgtttt tttatactat tttctttaat gttcaattat gaatgatgat ataattgaga
    39721 tagtaaagga gtgaaacctg tggctaaaaa agttagaata ggagacaaac tggtcgaaaa
    39781 aggatatata acagaagaac agcttaaatg ggcactatca gaacaaaaaa acagtggaaa
    39841 aagactagga gaatttctag tacaagaagg gcttatagat agtaacttac taataagtgt
    39901 attaaaagaa ctcttggata tagaaagtat atttttagaa gggacagaaa tagacacttt
    39961 agctacaaaa atggttccag aaaatatatg caaaagatat actgtatttc catttaaaat
    40021 tgatggaaat aaaatatgtc tagccatgtc agacccacaa gatagagaag cagttcaaga
    40081 tgtaaggcgt atgtctggta aagacgtaga gatatttata tcaagtacag aagatataaa
    40141 caaagcaata ggtcatgctt atgcacattc agaaataaat aaggcaatga ctgagtacaa
    40201 caaaaataga actggtggag taagagaaac agtaatactt gaagaagatg taaatgctgc
    40261 tccaatagta cgattagtaa ataacatact agaaaatgct gttagaatgg aagcaagtga
    40321 tatacatata gaacaaagtg aaaattacat gcgtgttagg tttaggatag atggaatgtt
    40381 aagagaatat atgaggatga actcagcacc atataaggca gtaatcagcc gtatcaagat
    40441 aatgtcagat ataaatatat ctgaaaagag aataccacag gatggtagaa tctatttaaa
    40501 agtagataat aaacccatag attttagggt ttctacaatg ccaactaaca gggatgaaaa
    40561 gatagctatg agagttcttg ataagagtaa ttttatggtt agtaaagaag tactaggaat
    40621 agacgaacat ggctctaaga tatatgatga attgataaac actccttatg gtcttatatt
    40681 ggttgttggc cctactggaa gtggtaaaac cactacattg tattcaatgt taaatcaact
    40741 aaatacagaa aatagaaatc tattaacaat agaagaccct atagagtatg aattacctgg
    40801 tgtaaatcaa tctcaaataa atgaaaaggc agggcttact ttcgccagcg gacttagagc
    40861 ttttatgcgt caagacccag atataataat ggttggagag ataagagata ctgagacagc
    40921 agaaatagca attagagctt ctcttacagg acatttagtt ttaagtacat tacatgcaaa
    40981 tacagcagtt ggagcaattt cacgtctttt agatatggat gtggaaagtt ttttaataac
    41041 ttcatctgtt ttaggtgtaa tatctcaaag acttacacga aaaatatgtg agcattgtaa
    41101 ggtttcatat gaagcagata ttggagaaaa aaaggctctt ggtattgatg taaatgaatc
    41161 agtaactatt tatagaggaa aaggttgtga aaggtgtaat aatactggat ataaaggacg
    41221 tctaggtata tttgaaatgc ttgaaattac tccagaaata aaagagttaa tagattcatc
    41281 tgcaaatcaa agagaaattt taaagatggc tagaaaacaa ggtatggtta gcttaaaaga
    41341 ggatatagtt aaaaaagttt taaatggaaa gacaacagta gaagaaatga ttagaataat
    41401 tttgatgact gattaaagga tgatattatg aaaagtttta aatacacagt aataaaagag
    41461 gacggaaaaa agcaaactga tgtgattgaa gccaacgatt ttaatgaagc tagaaatctt
    41521 cttagaaaga gaaatcttag agttattgaa atcaaagaaa gcaaaaataa aaatataaga
    41581 ttatctagta gaaagagaaa aaaagactta ggagctgacc agataagcca cttttgtaga
    41641 cagtttgcta ttatagtatc ttctggtata aatagtattt ctggattaga aacattagct
    41701 agaaggtcaa ctaacattac attaagagaa gaaattaatc gtatagtggc agaaataaaa
    41761 gtaggttcta ctattgcaga ttcaatgctt tcatcaaaat caaagtttcc aaagttactt
    41821 ggtgctatgg tagctacagg tgaagctaca ggtaaattag atgaagtatt aaaaagtatg
    41881 gcttcatttt atgcaagtga acacagggtt aaacaaaagc ttagaaatgc tgcaacatat
    41941 ccattaatag ttttgatttc ttcatttata atgattttta tatttacaac atttatggtg
    42001 cctaagatga ttaattcaat tacaacagta ggagcaactc ttccacttat aacaagaatt
    42061 gttatggggt ttggtatgtt tatgaagaag ttttggataa tagtactttt agttattata
    42121 ttatttatct atcagtttaa aaaatatcta aaaacgtcag ttggaagggc tcataaagat
    42181 agagttataa ataagatacc tgttttagga aaaggaataa attgtatggt agctacacga
    42241 ttttcaagag cattgtatct atttgtatca acaggatacc cattagttca agggcttgat
    42301 tatattatag atagtgtaaa taacaccatg gcagagaaga ttttagcatc agctaaagat
    42361 ggaataacaa gaggagaagg cttagcagaa aatttagaga gatatacgta ttttgactct
    42421 gtgttggtac agatgatagc tataggggag cagacagggg aattagagaa tataagtaga
    42481 cagatggcag aattttatga gtatgaatcc gagatttatt taaataggat ggcctcaatg
    42541 atagagcctg ttttaataat tgcagttggt attatagttg cgatactggt tgtatcaata
    42601 ttcatgccaa tgcttagtat atatgatgcc atgtaggagg gaaaggacaa atgaacaagg
    42661 tttatgtatc atattatggc gattctattt ccatagttga gggcagatat aaacacgata
    42721 aggataagtt ttatgtaaaa aattttaatg tgttttcaat agaggatatt gcacaagatt
    42781 tttctaagga aaagtatgct ctattgagat acgcattaga aaatgttaag ttaaaatcaa
    42841 gacatgtagt tttttgttta aatacaagag atgtaatttt aaaaccccaa aaacttccta
    42901 atatggacaa aaaggattta gacggattta tgaagctaga gatggacgaa atgatggctt
    42961 tggagtcaga tgaatatgta ttttcgtatg aagtatctag tgaaacacct gataattcag
    43021 atggcgatga atcaagttta aatcttatta ttggagctat acaaaaggaa gaggtaaatg
    43081 caattgtaga gataatgcat gaatttgatt tagtattaga tagaatagat acattatcaa
    43141 cagcttactt aagaatgctt aaaaatttag actacaatga tataatggta accaatataa
    43201 gtgacaatag cacaattata aatatatata agaaagatac tttatttatt agtgataata
    43261 tacctataaa gataactgct aaagatatgg acatgcaatt attaaatgta gtagaagaat
    43321 caaaaggtct tatgaattac tattcgtcaa gaaactttgg taaggttacg gatactatat
    43381 taatattagg aaaaagatat tccaataaat taatttatga aacttttagc tctgcatttt
    43441 caagctatgt ttctcaaggc cttactgaga taatggatat atctgatatg ataactggag
    43501 atatagatga agatgatata aattcaattg tagagctttt aggaagtatg atagaaccta
    43561 aagggaaaag agattttgaa aatataaact ttttaccaat ggatatactt agaaaacaga
    43621 taaaagctaa acaaatgaaa aacatactga aaattgctcc aatagttatt ttgattcttg
    43681 ctatacctta tttatgttta attttttcaa ataatttagc aaataaggaa cttgaaaatg
    43741 ttaatggtga aataagaaat attgaatctg catattatca gattggaaat atagaaaaaa
    43801 tgatacagag taaaagcgaa gaaataaagg tatatgatat gttaattggg aaaaaggtaa
    43861 agtgggaaaa tttattggat gatatagata aaaatactcc aagtaaagtt gaactaacaa
    43921 gtttatcaac aacttatcaa aatcttggaa ctaataataa agctgataat tcaaaaagta
    43981 ataagactac aacagataaa tcaagtaaca atgaaacaaa atcaagtacg aaaagtgata
    44041 gtaatggaga taaaacagat aatgatttaa atgaagataa gactacaaat gtgtcagaag
    44101 cattaactgg aaatagtgag actaatagta ataacacaga taaagatgat aaaactccat
    44161 tgtatgataa gataccaaat gttataaata tagaaggaaa tgctaaagat tcctcttatg
    44221 taggtcaatt tttatataaa ttaaagggat tatactattt caaggatgtt aaattacata
    44281 gcataaaaag taatgcagaa gattctaact atacatttag tatgacatta tacctgaaag
    44341 aaggtgtatt aactaatgaa taaagatata tttaaaaaag atataaaaga tatatttaaa
    44401 atggaagtta gtctaaaagg gattcttata aaggttataa ttttattagc tattttatca
    44461 tatggttatt ttggacttta ccctcaatat cagaaatata ttgctacaaa aaattctgta
    44521 aaacaggcta ctgaaaaatt aaatatatat aaagagaaaa ctgcacttat gccaaaatat
    44581 gaaaaacaac ttgaagaagt tgaaagtgaa ttaaaagaaa aaagcaaaaa tcttttatat
    44641 gatatgcaag atggtctttt tttaataggt ttagataaaa aaatgaaggc ttataatatt
    44701 gacttaacca gttatgacgt tggaaatgtt aaagaatacg aacattttta tggtatatca
    44761 acaacaataa gtgttagagg tgattataga aatgtcaaaa aattaatggc ttatttagaa
    44821 gaacaaaaaa atgttactca aatactagat tatacaatga cttcatatat agaaccagaa
    44881 aaagaagagg aagaaacaac aaacaataag gatttaatta aaaatgtgaa cataaataaa
    44941 gatacaaaaa tatactgggt ggaaggcgaa aataagtggc atttatataa ggattgtcca
    45001 ttgataaaag agggagagat tctaaaaaat gacaaacttt tagataggtc tactaattat
    45061 ctatgtaata cttgtgtact tagagctgca acagaaaaag aagcaaaaaa agaagtcgta
    45121 gctccaaagg cagaaggtgt agtagatgct acatttaaat ttataatgta cactaaagag
    45181 aatactacaa tgatacttga tacagataat ccatcttcat ggagaccagg aaaatataat
    45241 ccatttaagt ctagtgcgaa ataaagctaa gtaagcttta aagataggtg atgagtttgt
    45301 acaaaaataa tgaaaaaggt cttactttat tagaagtaat aatagctgtt tttatactga
    45361 caatagtttt aagtatttct tataaagtgt tcaatggtat aacatcagca gtaaaaaagc
    45421 aacagattat tacagatgct caagtaaata ttaatctaat taataagtat ctaaatagag
    45481 atttggaaaa ctgtaaagaa ctaactaaaa ctggttcagg taataattat gaatacaata
    45541 tagagatgcc agataatgtt gtaaaatatg aagtttctat agaaactaaa aaaaatacag
    45601 aggtatattc tgtaacgaga atccaaaaca atacaattga tacagaaaat gaagttagag
    45661 aagagataat ctataatcaa ccactagttc aaaacaataa agaaatgaag gaaacaccat
    45721 ttaaaataga aaagcaaact ggtaaatcta tttatactgt aagtatatac tataatgaat
    45781 cagtgcaaga aagtcataaa aatagcaatt taaataacaa aacttataca tttgatgtta
    45841 tgtcaagaat aggataataa gaatttatta aaaaataagt aggttgataa aatatgagta
    45901 aaaagaatag tgaaagagga ttttccttaa ttgaagtgtt agtagctatg gctattatgg
    45961 gaattgtact atttgcattt tttaatatta tcaatactaa taacaaagca aacactaaaa
    46021 atgacacaga tataacttcc ttaaactatg tccaaagtga aatagaaaat ctaagagaaa
    46081 agataaaaag tggagaattt gattttgata gtttagataa actagaagat ggaactgtcg
    46141 tatatgaaaa attaatagat aaatcaaaaa aagttgtata tgataaagtt cttagtgaag
    46201 gtgatgtgag cttatatgat actccatatg aaaagattac aacaataaaa gatgaagatg
    46261 gtaatttaat cgacaaagga aatattacaa ataagataaa gactattgta gaagataaaa
    46321 gtggtcagat atataaaata gctgtaactg ggaaaagcat gaatgactat tcaagtaaaa
    46381 aagaggttaa aattgtaact gaaatattta aagataaata attttacttg ggggtgagat
    46441 tttgaggaag tggaataaat ttaaaagtga aagaggggca gctttagttt tagttttggt
    46501 tgtagtagca ttgctttcta ttgttggatt gatattttca aatcagatag ctaatagaat
    46561 aaaatctaca aagactacta atgaaggtat acaagcaaag tatttagcag agacatgtgt
    46621 ggaaaatagt attgataaag catatgaaaa attgtatgat gagcttgaga aaatggataa
    46681 tgagtttaaa agtgaaaatc aagaaaaatc catatctagg tcaaaattaa gagatatatc
    46741 agatgaagat tttaataatc aagatgagga aaatattgaa gcagaaagac ttggatatat
    46801 gaataatata aatttctatt taaataaagc ttcttcagac ttggaaaaag ctagtatgga
    46861 attgaaaaag ctatatgatt tagatatgtt ggactataga gacattgaat atgtagatgc
    46921 aaatattatt tcacatagag attctatctt agaaatttgc aagaattata cttctggaga
    46981 tattagtaaa ataaatgaat atattctgaa agaagatata gactcaacta cattgattga
    47041 ggcaaagtta gtgaataatg atattctttt aaaaatgttt ttagaagaaa ataaaattga
    47101 aaatgaacat ttaaattcag ccttttcaca tacttataag gcattagata acatttcttt
    47161 ggcgatgcaa aatatgattg agtatagaca tacgtttcat atagatgagc caaaagtaga
    47221 agtatctaat gggattccag attctcaaca atattatgaa ctaatacaaa atcctataat
    47281 aaatagtatg gagtatatct ggaatagtaa atgggataca ttagaaaaac tattggaaat
    47341 tttaccaaat caaactcaag gatttaattc tttaagagtt catttgagaa ataatgtaag
    47401 aaaatttgag aagttaagcg ataatatatc tagtggtaaa aaaaatacag caaaaaattt
    47461 cttaaagtat aaagagttat tgtatgaaat atcagaccaa tgtaatcaac taaaaagtat
    47521 gtcttatgag aaaatacctg ttaaatatga taacatggct ttgataacaa cttttgacta
    47581 tatacaaaat gagcttttag cagagataaa atgcagatta aaagaattaa aaccacaaga
    47641 aattgataaa acagaaggta ttactataaa aatacctttt tataaggctg actacgacat
    47701 gacaaaagaa ggatggccta aattaaaaga aaatggttca ggggcggagt taagcttaat
    47761 ggtaactggt gataaagatg gaataaaaga agtagaagtt acagatggta aaaaaaatat
    47821 tattggttta ggtgttgaag aaaattcaaa ttcaaagtat aaggttgacg ccattgtaaa
    47881 ctttaatcta aatatagata caaatgtagt tggaaattat gatataaaag ataaaatttt
    47941 gattaatcat gatataagtt catacaaaaa agtaaactaa atataagaag caacttagta
    48001 taaataaata ataaaataat tttattattt actactaaaa aaataagtaa actaatagaa
    48061 gatatggaga gtaaatatta tgtatattta tgatttatta gagcaaggta taagactaaa
    48121 cgcatcagat atacacataa cagttggaac aaatcctgta gctagagtaa aaggtggatt
    48181 tgtaaaacta agtgaacaaa tattgacatc agaagtaaca atgcaaatgg caaaagacat
    48241 agcaggggaa tctatgttta aagtaataga agaacatgga gaagctgatt tttcagcttc
    48301 tttaaaaaca ggagaaagat ttagggtaaa tgcatatagg caaaaaggaa attacgcaat
    48361 agcaataagg actataacag cagaaattcc aacatttgaa aaattaggcc ttccagagag
    48421 tataaaatct tttactgaaa agcataaagg attagtttta gttacaggtc caacaggaag
    48481 tggtaaaagt acaacacttg caagtatgat aaatataata aatgaaaaac aacaaaaaca
    48541 tataattaca cttgaagacc cgatagaata tgtacatcat cataaacaaa gtttagtaaa
    48601 tcaaagagaa gtaggaactg atacagaaag ttttcattca gcccttagag ctattttgcg
    48661 tcaggaccca gatgtaatac taataggaga gatgagagac cctgaaactg tttcaatagc
    48721 attaacagca gcagaaacag gtcatttagt attttcaaca ctacatacag tgggggcagc
    48781 taagacaatt gatagaatag tagacatgtt tcaaccatcc caacagcaac aaataaaaac
    48841 acagttatct acagtttgtg aaggtgtggt atcacagcag ttacttccta cagcagatgg
    48901 gaaaggcaga atagcagcaa ttgaattgat gtttgctact ccagcaataa aaaacctgat
    48961 aagggaaggc aaaacatatc aaattccaaa catgatacaa actggagtta aagtaggtat
    49021 gaaaactatg gaccaagatt taatggaact ttataaaaat ggaaagatta caaaagatat
    49081 ggcacttagt aggtgtactg accaagagtt tatgacaaga atgataggtg gagtaaacta
    49141 caatggatat tataatcgtt aatctatatg tatttatggt gggtatagtg tttggaagtt
    49201 tctttaacgt ctgtattttt aggattccag aaggtatatc tatttctaga cctccttctc
    49261 attgtatgag ttgtaatact agactaaagc caatagactt aataccagtg ttaagctatt
    49321 taatgagtgg cagaaagtgt agatattgtt atgagaaaat ttctagtaga tatgctatag
    49381 tggaattaat tacaggactt ttatttttaa cagtatttat gttatatgga atatctacat
    49441 caactattta ttatttggtt cttgtgtctt tactgattat aattacattt atagatattg
    49501 accactttat aataccagat aaaatattgg tttttggagc tatattttct atgattttta
    49561 accttttatt taaagaaatt ccagttaagg attcaattct aggtgttttt atttgtggag
    49621 gaagtgtctg gattatagtt cttttgattg aatttgtaat aaaaaaagag tgtatgggtg
    49681 gaggagatat caaactattt gcaatgctag gtttttacat gggtgttaaa aatggtcttt
    49741 tgacagcctt actaagcgtt tatgtgggag cagtctatgg aatctgtgta ataattataa
    49801 gtagaataaa aggaaaggaa tataattctg ttataccata tggacctttt atatctattg
    49861 gagcattaat aacaatctta tgtggaaagc aattacttga actttatttt tctatggtta
    49921 tatagatgat agtcaagtta aataaagttt taatttaaaa aaatgaatta aagtatttaa
    49981 taaggacaat attattatta accctattac taaagtgcta caaactatgg agtatataat
    50041 aataatttcc ttattttttg cacatttgat aaataaatct ctatgtgttt tttgtgatag
    50101 agaaaagaaa aatttataca atacgttttt tatttacata ataactgtag ttttatctgt
    50161 attaatatat ctaaaatttg ggattagtgt agaatgtgta caatatatgg ttttaattcc
    50221 atttttaata attatatcca ttgtagatta ctacacaata tatgtatatg atataacaat
    50281 agttagtggt ataatagttc aaggtataat ttttttggtc tttttcaata tagttcaatt
    50341 tgattatata aatcatatat ttggactatt gataggcttt attatacctt atattattgc
    50401 taaactaaca aaaggattag gctatggaga tgtaggtctc tatagcttat gttgctttgc
    50461 attgggaaat aattatgggt tttatgtgat agttttatca tttattttag cgtttttgtt
    50521 ttttattatt ttatacataa ctaaaatatt taaagatgta gatataagaa agcaaattcc
    50581 ttttacacct tttatttcct tgtcaacaat tttaataata tttacagagt ataatatatt
    50641 taatttttat tataatttgt tatacaaatt tatataacag gaggtaagaa atgtacgtag
    50701 tagtagggtt aggtaatcca ggaaagaagt atgagaagac tagacataac gttggatttg
    50761 atgttataga tattcttgct aaagaataca atattagtgt tactaaaata aagcataaag
    50821 ctttgattgg tgaagggaga gtaggtactg aaaaagtatt attagttaaa ccacaaactt
    50881 atatgaattt aagtggagaa acattaatag atatatataa atattataag gttgatttaa
    50941 gtaatatagt agtagtatat gatgatatag atttagaagt tggaaagata cgtataagaa
    51001 aaaaaggaag tggaggaact cataatggca tgaaatctat aactaagtgc ctaggttcaa
    51061 atgactttcc aagagtgaga gtaggtgtat ctaaaccaga agctggtcaa gacttagcag
    51121 actttgtact ttctagattt agaaaagaag aatctgataa cataaatgaa gcattagaaa
    51181 aagcggcaga tgctattgac tcaataataa gagaaaatat agatatgtct atgaacaaat
    51241 ataatggata gtgagaggga gatttaaatg aatgatgttt ttttatatcc tttgcaaaat
    51301 tcaaaagagt ataaagatat aataaactgt ataaaaaata caaaaggctc attattggtt
    51361 aatggacttt taccagtaca aaaacctcat atatcgtatt ctatatttaa tgatttatca
    51421 cgacaaatga tttttataac aagtagtgat ttagaagcaa agaaagtata cgaagattta
    51481 agtttttata tggaagataa agttgaatat ttaggttttc aagatattta tttttatcac
    51541 ttggatgcaa aggacagaaa cgaagaagcg aagaaattaa aagtattgtt aaaactagca
    51601 aataaagaaa aaatcatttt agtaacttct attgaggcag ttttaagaaa atacatacca
    51661 aaacaagttt tgctcgatag tgtatctcat tataaggtgg gagatagctt agacttagaa
    51721 aagttaactg agaaattagt ttcattggga tatgaaagag tttcaaaaat agaggggttt
    51781 ggtcaattta gtataagagg tggaattatt gatgtatttt cacttgaata tacaaatcca
    51841 attcgtatgg aattatttga tgatgagata gattccatac gaacttttga cgtatattca
    51901 caaaaatcaa ttgataaatt gcaacaattc tctataactc catctaggga atttatttat
    51961 cccgaaaaaa ctacagatgc attagttaaa ttaaaaaaag aaacaactaa aaatactgat
    52021 gaaggtacat ttcaaaatat agattatata tcaagtaaga cttattttga aggtgtagag
    52081 aactatatag attatattta tccagaagaa aacaaaagta tatttactta tttagcagat
    52141 gatgcaatag tatttattaa tgatataacg agacttaaag aaagatgcga aaattatata
    52201 aatgagttta gagaaaatta taaattaaat cttgaaagag gtctggctat taaaaaccaa
    52261 ggtaaattgt tatatcacta tacagattta gagtacttag taaaagacaa gtctgtggta
    52321 ttaaactcgc tattgccaaa gtctataaat aacttttcta taaaatctat aataaatttt
    52381 gaaagtagag aagttcctac atttaatgga aaggtagatt tgcttgtaga agaattaaat
    52441 cggctgaaat ataatggata caaaataatt ttagcaacaa ataccttaga aagagcaaat
    52501 aaattaggaa aagatttatt ggacaaaggc ttagaaacga ccatttctaa agatagagat
    52561 attgaaatta aatcttcaca agttataatt gtacctgcac acataaacag tggttttcag
    52621 tataaatcta taaagtttgt tgttataaca gataacgaaa tgataggtgt ttataaaaga
    52681 gcgtctaaga ctagcaataa aaaagttaaa aagggaaaga aaatagaatc ctttttagat
    52741 ttaagtgttg gagattatgt agtccatgaa aatagtggtg taggtagata cacaggaatt
    52801 gaacaaatta cagtcaatgc tattaaaaaa gattatatga aaatagtata tcaaggtgga
    52861 gataatctat atgtgccaat agaccagatg gacaaagtac aaaaatatat tggtgcagaa
    52921 gtagaaaaag ttaagttaaa taagcttgga acaaatgagt ggacgaaagc aaaagcaaaa
    52981 gttaaaaaag aaattgaaga tatgacaaaa gacttgattg agctttatgc aaaacgtgaa
    53041 aaaatacaag ggtataagtt ttcaaaagat acaccttggc aggcagagtt tgaatcgttg
    53101 tttccatatc aagaaacaga agaccaatta aaggctatag aagaaactaa aaaagatatg
    53161 gaatcaagta aggtaatgga taggctagta tgtggagatg ttggttatgg aaaaacagag
    53221 gtagctatta gaagtatttt taaagcttgt atggaccaaa aacaagttgc tgtattggtt
    53281 ccaacaacaa tattggccca acaacattat aatacattca aagagaggtt tgaaaattat
    53341 cctttgagag tagaagtttt aagtaggttt aaaacaccaa aacaacaaaa acagattatt
    53401 gaagatgcta aaaaaggatt agtagatatt ttaattggaa cacatagaat aatatccaaa
    53461 gatataaatt taccaaactt aggtcttgta gtcattgatg aagaacaaag atttggagta
    53521 aaacataaag aagctttaaa gaaaattaaa tctacagtag atgtacttac actttcagca
    53581 actcctatac caagaacatt acatatgtct ttaagtggaa taagagacat gagtgttata
    53641 gaagaacctc cacaagaaag acatcctgta ataacatatg ttacagagag taaggaaagc
    53701 gtaattcaag atgagataga gagagaatta agtagaggtg gacaggtatt ttttgtatac
    53761 aatagagttg aacacattga agaaatggca agtatgattc aaaaactcgt accagatgct
    53821 agagtggctg ttgcacatgg aagaatgact tctaaatcat tagaaaatat aatcctagga
    53881 tttttaaata aagattatga tgtactagtc tgtactacaa taattgaaac tggtatggac
    53941 atatcaaatg caaatacaat gattatatat gatgcagata aaatgggatt ggctcaactt
    54001 taccaattga gaggtagggt tggaagaagt tcaagacaag gatatgcata tcttttgtat
    54061 gaaaaagata aaactttaag tgagatagcg gagaagagat taaaagctat cagagaattt
    54121 acagagtttg gttctggttt taaaatagca atgagagacc ttgaaataag aggagcagga
    54181 aatattttag gttctcaaca acatggtcat atggcagtta taggttatga tttgtacgtt
    54241 aaaatgctta atgatgcaat taaaaaagtc aaaggtgagc ctattgtgga ggaaattgat
    54301 gttgaaatag atttaagtgt aaatgcatac attccagata attatatcaa agatgagttg
    54361 attaaaattg aaatgtataa gaaaatagct tctattgaaa acaaagaaga catgttggat
    54421 atacaagaag aacttgagga tagattctct gatataccta aaccagtcca gacacttctt
    54481 acaatagctt atataaaatc gttatgtaag atattaaaaa tagaaaagat aagacaatta
    54541 aaagatgaaa tattattagt tccaataaca aagtatagaa ctaaacagaa aataggttat
    54601 aatatagtta cagaattaga agaattatta gaaaagatgt gcaaggtaaa ataacttgta
    54661 aatttaagaa gggatgtttt aaatttgaaa aaagtaataa cattggtaat agctatgatt
    54721 ttggtagtat ctgtgactgc ttgcagttct agtaaaggag aaactgtggc aacagttgaa
    54781 ggtacaaaga tatcatcaga tgaatttaaa aaaactatag ctctttataa agattctatg
    54841 gaacaaactt atggaaaaga tatatgggat aaggaagttg aaaagggtgt aaaatacaag
    54901 gacaagttta aagatttaat tcttgaccaa cttataacta cagaagtaat atattctcaa
    54961 gctaaaaaag ataacttact tcctaaaaaa gaggatgttg aaaaaagttt taaagaatta
    55021 aaagacgcta tgggtaaaga tgaaaaatat aaagaacaac ttaagaaatt aggtatagat
    55081 gatgagtttt taagagacca acaagagaaa gatttagcaa tgcaaaacta tcaaagtaac
    55141 tttgctaaaa agactaaaat atcagatgaa gaaatgaaaa aatactatga tacacataaa
    55201 gatgaattta aaaaagatga ggtagaagct tctcatattt tattgaaaac agtagatgat
    55261 aataacaagc cactttcaga taaggaaaaa gctgaagcta agaaaaaggc tgaagaggct
    55321 cttaaagaag ttaaatcagg tgaggatttt gcaaaagtag caaaaaaata ttcacaagat
    55381 acttcagcaa gtgatggtgg taaattagga ttcttttcaa ggggtcaaat ggttgctgaa
    55441 tttgaagatg ctgctttttc tatgaaaaag ggtgaagtat ctgatttagt tgaaactcaa
    55501 tatggatacc acataattaa agtgacagac agaataaatg agcaaacatc atttgaagat
    55561 gctaaagaaa ctataaaaga ccaactgttg aaaaataaat atcaagaaca aatagaaaaa
    55621 ttaactaaag aagctaaggt agaaaaagat gaaaaagtaa taaataaaat cacaatctaa
    55681 gacttaaaaa aagagtaata tgcttaaaaa agctatatct ggataagata tagctttttt
    55741 tgtatatttt tcttaacaat ggtaaaaata aacttaaagt aatttatgga gggattaaaa
    55801 aatatgaaag caacaggtat agttagaaga atagatgatc ttggaagggt agttatccca
    55861 aaagagataa gaaagacatt aagaattaga gaaggagacc ctttagagat attcacagca
    55921 aaagatggag aagtcatact taaaaaatac tctccaatag gtgaattaaa tgaattttcg
    55981 caagaatatg ctgaaacatt agctgaagta ctaggatatg gagtgttgat aacagactta
    56041 gattctataa tagctgtgtc taagcttcct aaaaaagatt ataaagaaaa aagtataagt
    56101 gatgagttag aagcaataat aagtgaaaga acagtaaaat attcaaaaga taaaaacatt
    56161 ttaccattat tcaaggaaga ccaaaaagaa tatactagtc aagttatcat gcctataatt
    56221 agtgcatctg gtgactgtat aggttcaatt gtagttgttt caaaggataa agatacaatt
    56281 gcagaaagtg aagaaaaatc tttaaaaatt gctgctaact ttttaggaaa acaagttcaa
    56341 taattttatc ataaagacct tttacatgaa ggtcttttga agtttttgtg taaaatattg
    56401 aatatagcat taaaaatatg gtatattaaa gtttgttata taaacagtat ggaggttatg
    56461 ttatgaacaa taacaaaaat aaagactcct ttctaaaagg agcccttata cttgggctag
    56521 caggtgtaat cgtaaaaata atgggtggat tttttaggat tccccttgga aacatgattg
    56581 gttcagaagg aatgggatac tatcaggctg cttatcctgt gtatactcta tttttaacat
    56641 tagctactgc tggatttcca acagctgtgg caaaattggt ttcggaaaaa gttgcaattg
    56701 gaaattttaa aggtgcaaat aagatattta aggtatcaca tacagtttta ttcattaccg
    56761 gtataatatc attttgtata ttattttttg gagccgacta catagtaaca aacgtaatga
    56821 aaaatccagg tgcgttatac tctatgaaag ccattgctcc agcattacta tttgttcctg
    56881 caatgtctgc atatagagga tattttcaag ggagacaaga tatgactaag attgctgtgt
    56941 ctcaagttgc agagcaattt tttagagttg tattaggtct tactttggct tacttgctta
    57001 tgaagagtct aggtcagact tttggtgcag caggtgctat atcaggggct acaataggtt
    57061 caatagcatc tatgctttat ttggtgtttg cttatatgct agggagaaaa gagcgtagag
    57121 cagagataga tgcaagccaa agatttaaag atgaaagagt gtcttatatt tttaaaaagc
    57181 ttttaactgt tgccattcct ataacaatag gagcatcagt aatgccactt gtaaatatga
    57241 tagataatgt tattgtaata agaagactta tggaagcagg ctttacatac aaagtggcaa
    57301 attccatgtt tggtcaatta acaggtatgg caatggccac aataaattta ccagcagtca
    57361 taacaactgc aatgagtatg agcttagttc cagcaatttc taaagcatat gcattaggaa
    57421 ataagtcaaa agctagaaaa gatactaaga gtgcagttaa ggtaacttta cttatagtat
    57481 tgccttgtgc gtttggtatg gcttctttag caataccaat aatgggtctt ttatttccac
    57541 atgaaccatc ttctgtagga actattttgt ttactcttac tccatgtgta ttatttttag
    57601 gattaataca gacattgact ggtataattc agggaatggg aaaaccaata gtacctgtaa
    57661 ttgctttatg tgttggtatg ctatgtaaaa ttgtaataag ttatacttta acaggtatac
    57721 cagatattaa tgtactgggt tcagcatttg gtacagttac agcatacttt gtggcagcca
    57781 tgattaactt actatatgtt aaaaaacaca tgaatgtaaa cttttctaaa aaagaattta
    57841 taataaagcc atttataaca gttatgacaa tgtttataat ggttaagttg agttatggtg
    57901 ctttagttgg attcttagga aacagcatat ctacaataat tgcaatatgt gttggtggaa
    57961 ttgtatatgt agtagtaata cttggaatag gtggaattaa aaaagaagag attctaacta
    58021 tgccaaaagg tgataaatta tataaattgt taaaaaaagt taaacttata agataatata
    58081 ggaatatata tgcaaataaa ggaaatacta tacaaatagt aaatctcctt atttgtataa
    58141 taatataaaa ataaagtgaa atttgctaat aggagtgatg ttatgggtaa gataagtatt
    58201 gtaggtcttg gaccaggaga ctattctctt ataagccaag gtgtgttaga atctttaata
    58261 aatagcccta aagttttttt tagaacagag aaacatccaa taattgataa acttaaagaa
    58321 actatacaat acacttcttt agactacttt tatgaaaaag aggaaaattt tgataatgtt
    58381 tatttgaaaa tatctgaatt tatcatagaa gaatcagcaa agggtgacct agtgtatgct
    58441 gttcctggtc atccaagagt tgcagagaag actgtaagta taatagagag attagccaat
    58501 gaaaagcaca tagcagtaga aatattgcct tctatgagct ttgtagacgc tatgtttaac
    58561 tatttggcta tagacccatc tgagggattt aaactggtag atgcttttga aatagaaaat
    58621 tcatatatag acttggatac aagtatgata ataactcagg tatatgataa atttatagca
    58681 tctaatgtaa agttaaaact tatggaacat tatgactata atcaagaagt atgtatagta
    58741 aatggagctg gtgtcaaaga tttagaaagt aaaaagttta taaagctttg tgaattagac
    58801 agaaatgaaa acttttttga ctatttaact agcttatata tacccaaaag ttctaaaaag
    58861 atgtataata cagtacatga cttagaaata atagttaata cacttagaag tccatctgga
    58921 tgtgaatggg ataaaaaaca aacacatcaa tcattaaaaa attctgttat agaggaagca
    58981 tatgaacttt gtaatgcaat tgataataat gatatagatg aaatggtaga agagttaggt
    59041 gatgttcttt tacaggttat attccattgt cagataggaa atgaggaggg ctactttgat
    59101 ttaaaagaag tagtaaatgg catatgtaaa aaattaattc atagacatcc tcatatattt
    59161 agtggtgcaa aattagatat gtctaagttt gagaaaactt gggaagaatt aaagaaagaa
    59221 gagaaaggcg aaagtactat aacagatggc cttagaagga taccaaaaca tttaccagct
    59281 cttatgaagg ctagtaaagt tcaacataaa gcagcacttg tcggctttga ttgggacaat
    59341 gtagaagatg tatttaaaaa aattgaagaa gaatatcagg aattacttga cgaacacagg
    59401 gtcgggaata taaaatacat aaaggaagag ctaggcgatt tattattttc gatagtaaat
    59461 ctagctagat ttttagacat agactcagga gaagccttga actgtactac agaaaaattc
    59521 ataaatagat ttgactttat agaaaagagt gcaatgaact tgaataaaaa attagaagac
    59581 atgacacttg aagagatgga tgagttttgg aatcaggcta aaaaacaata aaattgtgga
    59641 aggaaatttg tcaaaaatct agaactttta tcacaaagat agaagtctat attataaaca
    59701 tttttaggag gtaaatatcg tgaataaagc tgaattagta tcaaagatgg cagaaaaaag
    59761 tggattaaca aagaaggaag cagaagctgc gttaaacgca tttatgagtt ctgttcaaga
    59821 tgcactagta aataatgaaa aagttcaatt agttggattt ggaacatttg agacaagaga
    59881 aagagctgct agacaaggaa gaaatccaag agatccagag caagttatag atataccagc
    59941 ttctaaagca ccagttttca aagctggaaa aggattaaag gatataataa atggataatt
    60001 agaaatcttg tgaaaaagtg tggataaatt tgccacactt tttctataga accaaggaag
    60061 gtgataatta tgagattaga taaattttta aaagtatcta ggattataaa aagaagaact
    60121 gttgctaaag atgcatgtga taaagggatt gtcactataa atggaaaagt tgctaaatct
    60181 tcttcagagg tgaatattgg agatacatta gaaatacaat ttggagagaa gaagatgaag
    60241 tttaagataa atgaagtaaa agagcatgta ttaaaaaatg atgcaaaaga aatgtacgaa
    60301 attttagaat aaaattaata gtcctctagt atattaatag tagtattatt gaaatattta
    60361 ctggggggat attatggaac agaacataac tttgaaagat agatcaaagc tggttatttc
    60421 gggagtcgag cacatatact cttttaatga caaaagagta gagctcaaaa cttctgtagg
    60481 cgagatggta attgaaggcg aaaacctaga tatgagcaag ctaagtatag acgaaaatat
    60541 aattagcata gatggaacta taaattctat ggtatatgca aagcctaaaa agccacaaga
    60601 gagtttcttg aaaaaggtat ttagatagat gatacctttt actcaagatg taagcatttt
    60661 ttatgctact atttatggtg gaatactcat aggggtgttg tttgattttt atagagggct
    60721 aagagggaat tttaaattca tcaattattt tgctataata tttgatgtat tgttttggtt
    60781 tctagctacg gttataatat tcgtaacaat aaaccttaca gaattttttg atttaagata
    60841 ttatcatttt gtggctttgt ttattggttt tattttatac tataacacca taagtaaaat
    60901 tgttttatca attatcaata aaataattcg ttttgtgaga aattctttta aaaaagttac
    60961 acattacata gtcagttttt taaataattt gtattacgtt ataatctatt cactacacct
    61021 actatttgat attatatttt atataccaaa catatttata gcaacaagaa aaagtataaa
    61081 aagaaaatca aataaaaaat taaagaacaa gaaaaaatca aaacctaaga agaaaaaaaa
    61141 taaaactaaa aaaagggtgt aggaaaaatg aacttaagaa aaaaattttc aggacagatt
    61201 attgttatca gtttattttt aggaatatca atttttagta tgatgacagg atttgtattt
    61261 gagtatacaa aagctaagga atataaaaaa gaaatagcaa gtctaaataa acaattaaaa
    61321 aagactgaaa tacaaataaa cagcttaaag aaagatgaaa aatcatatga aggagactta
    61381 gaggatatag caagaaaaag attgaatatg gtaaaaccaa atgaaacagt ttatgtagac
    61441 ataaatcgtt gattaaaact tgctaataag tttacagatg tattgatttt aaaatttaag
    61501 atttaataaa aaagtcattc agttaaggat ggctttttgt ttttattgag gagggaattt
    61561 cataatgaaa gtaggagcta tagatattgg gacaaactca atgagattat tgatagcaga
    61621 ttatagagat gggaatttta taaacagaaa aaaatttgtt aatacaacta gaattggtta
    61681 tggtgtggat agtaaaggat atataaaaga agatgctatc aaaagaaata taaaagcatt
    61741 aaaagaattt tctgataagt gtaatgatga aaagtgtgaa aaaatttatt gtatggggac
    61801 atctgctctt agagacagta aaaatggaga ttactttgtt aaattagcta aagaagaaac
    61861 tggaatagat gttgatatag ttacgggaat agaagaatca aatttgggct ttaagggtgt
    61921 tttagaagga atacaaggtg atgatactat tattgttata gatattggag gaggttcaac
    61981 agaatttatt gtaggtgatt cagaaggaat tatatttaat aaaagtgaga atgtaggtgc
    62041 tcttaggatg acagagaggt ttttgaagca cgatattatt gaagatgaag agtttgaagc
    62101 tatgagttca tttatatatg gtgagattga taaaacgtta aatcatataa aagacaaagg
    62161 attaaaaact cttataggta taggaggtac gattacatca ttatcagcaa tgaatcaaga
    62221 attagagatt tactctatgg aaaaaataca taatagtaaa atacacaaaa aagaaataga
    62281 agaaattcta caaaaattaa taaagatgac attaagtgat aaaaagaact taaaagggct
    62341 acaagaaaaa cgtgcggata ttataactgc tggtgtaaaa atattgaata taataatgga
    62401 aaaattagaa atagaagaaa taaccgtaag tgaatacgat aatttggaag gcttaatatg
    62461 tcaaaagtca aaaaaaatgt attaatattt tttgattttt aaaaccgatt atgacaaaaa
    62521 aaattactcc tctttgttac aattcttaat ataaacaaaa gaggaggttt ttttatgcaa
    62581 agaagtgtag cagttgtgaa gaataagact gtaaaaccaa atgtcataaa attaataagc
    62641 ggatatattg attttactac ggcggcattc gctgtgatgg gatttttact tagtaggtct
    62701 attatagtag actctgtagc tcctttagga attgcctttt ttatatgtgc atcaaagatt
    62761 gataagtaca agatacctgt attcatatca acattattag gaatattgtt atcatctaat
    62821 gattttggaa atgtagcaaa atatgctgtg tgccttacaa tatttatggc tatgaataaa
    62881 aaattaaaat tatcagattc aattgctaag gttgcattta taggtgcaat aatactactt
    62941 ccaatctcca tgggacaagc tttcttttca aataggtatg tatatggtct tcttatagca
    63001 ggagtagaag caataataat gtttatatct atatatgtgt tttcttatgg gataggttta
    63061 atggccaatg tcaacaatag gatgtctgta cggacagaag aagccatatc aataagttta
    63121 ctgctaacct ttagcataat gggaatagga gatatatctc tgtttggaat atcaataagg
    63181 accattttat cgaccgtatt gatattgctg acctcaatag taggtggagc tactatggga
    63241 gcatctagtg gtgtaattat tggtgtagca tccattttaa ataatatgac atcagctgtt
    63301 tatatgggga tatattcttt ttcaggatta ataagtggag catttaataa aataaataaa
    63361 tatttctgta tactaggata tattctaagt tggacaataa tatacctata tacttctggt
    63421 attacttcta atatgatgca attaagagat atactattag gttgtttgat agtactagta
    63481 ttgccagagc gattatttaa taagattgaa aaattaataa aaagtaatgt ggcttcaaat
    63541 gaaatcgtat acgattatat tatgagaagc aaaaacctaa ctaatagcag attgaacagt
    63601 atttataaaa cttatgatga ccttgcagat acatttgata agataagaga aaaagacaaa
    63661 gtgctagacc aaagagacat agctaatgta atagatatga ttcataatga tgaatgtaaa
    63721 agttgtagta tgagaagaat gtgctgggag tcaagattta atcatacata cactatgata
    63781 tataacattc ttgaaaaaat tgaggagaaa ggggagttaa gtctaaacga tatacctaaa
    63841 aattttagaa aggaatgtat gaaaccagag tcaatagtga aaatatcaaa tcactattac
    63901 aaaatgtttg tattagatta tgattggagt gtgaagtttt cagaaagtag aaagttaata
    63961 gcaaatcaga taagaagtat ttcaaaatcc attaaatcct tatctcaaga tttagaaggc
    64021 gacataatgc tagatataga aaaggaaaaa aatatatatg aacagttaga aagatatgat
    64081 ataacagttg ataaagtaag ttacttaaca aaaagtaaca gtgagtttga aatatcaata
    64141 gaaaagaaaa cttgccatga tggatgtatg tgtgaagata aaattgtaaa tataatttca
    64201 gacctagtag gggaaaatat gtctgttaga aaaataggat gtcactgttt aggaggtaaa
    64261 tgcaaggcaa cattcgttaa atctcaaaaa tataaggctg ttacagaagt ttcagcaatg
    64321 tcaagggatg ggcatatcct ttgtggagac aattacacat acatggagat aaatgatggt
    64381 aaatacatga tggctataag tgatggtatg ggaaaaggta aaaaagcata tgaagaatct
    64441 tctgccacta ttgatatact ggaaaaaatg atagatgcaa aaattaaaga tgagatagtc
    64501 attgatacta taaataatat gttgttgtta aagtcttcag aagagatgtt ttcaactctg
    64561 gatttgggaa ttttagattt aaaacgaggt tgtttagaaa ctataaagat gggagcgtgt
    64621 tctacttata taaaaagaga ggatggagaa gttgacttaa tatcgtcatc atcattacct
    64681 gtgggtatat tatcagatgt aaaaattgat agaaaaaatg taaaagttaa ggaaggagac
    64741 tatgttataa tggtttctga tggaattgta gatgcaggaa gaaataacaa tttaggagac
    64801 aattggttaa tatacttctt aaagaatata gagactacaa atccaaagga aatttcaaat
    64861 ttaatattgg atagagcatt agagttacaa gcgttacaaa ttgaagatga catgactgtt
    64921 ttagttacga aaatatgtac aaaataaggt ttacaagatt atgtgtataa gatgtggata
    64981 acttgtgtat aatatgttaa tagacttttg aaaaaagtat agttattaac aaaaataaca
    65041 tataaatcaa caaaatttgt tgataagtat ggcagaaatg ttaataaaat gtaaaaaaag
    65101 aaaaaacaaa agctattatc aaaagagatt catgttgaaa taactaaatt tctaagtaaa
    65161 gaaaacctgt gaatatgttg attattatgt ggataactat taattcgata tttatttata
    65221 tagtatgaat aattgaataa tatatctaaa aaaagccatc ctattattaa gtaaacttat
    65281 tgaaaggatg gctttttatg aatagaaaat tgtgcaaagc aataatacct ttatttattt
    65341 ctattttttg catgttaata ataagtataa aagctgacca aggatatcaa aagaataatg
    65401 atagagcaat tactgtcatg aataataatg atactgaaga tgatgctgaa aaaaaacaag
    65461 aaatagacta tgaaaaaaca tgttggaatt tgcaatcttt gtttaaaagt gatgaccaat
    65521 ggaaaaaaga acttaagagt tttgataaag atactaaaga gttgaaaaat tacataggta
    65581 aagtaacaaa atcgccaaca catttgtctt ttgccttaaa cattaaagaa aaacttgata
    65641 taagactaaa taaactttct gcctatgtaa aattaaaaca agatactaat aaaaattcgt
    65701 ataagtactt agatatgaat gatagtatga gtaaatccta tgggaattat atggggattt
    65761 gttcagattt agagttagaa atactaaaat tatcaaacaa agattacaag aaaattattt
    65821 caaataaaaa tataaatagg aaatatggtg catatttaag agatataaga aggaataaag
    65881 tacattattt ggatgataaa tcagaagata ttctaagcaa agtatcttca ataagtagtc
    65941 ttccaagcca agtatatgaa ttatttagaa atatggatag aaaaactaat ttgacaccag
    66001 ctcaatatgc gagtgaatta gagtcatcag atagagaaaa tagaaaaaaa gcttatcaag
    66061 atgaacttat aacttacaat gataatataa atacaattgc aggtttaatt actggtcaag
    66121 ttaaaaagaa tattttttat tctcaggaaa gaggttatga gtcttcttta gaaatgtacc
    66181 tggaatctga taatgtagat gaaaaagtat acaataattt gataaggact gtaaatagta
    66241 atatggacag tctacacaaa tatatagatt taagaaaaaa agtattaaaa ttagataagg
    66301 ttcattcata tgatatgttt gtgcctatgg ttaaggctgt tgataataat attaattatg
    66361 aaaaggcaca aagtatagta tattcagcgt tgtcaccttt aggaaaagaa tacaatgatg
    66421 tagtatataa agctttcaat gaaaaatgga tagatgttta ttcacatgaa aataaagtaa
    66481 gtggtggata ctgcttatct gtttatgaaa atcatccata tatactttta aattataata
    66541 attcattagg ttcagtttcg actttatcac atgagcttgg acatgctgta tatgaatatt
    66601 taagttctaa gaatcagaat tattttaatt caaatccgtc tatatttact cacgaagttg
    66661 catctacaac gaatgaagca ttactttacg agatgcttat aaaagaagct aaaggcaatg
    66721 atgaaaaagc ttattatata acacagtatt tagacttgat aaaagatact ctttatacac
    66781 aaactatgta tgctgaattt gaaaaaacta tacacgagtt agtagaaaaa ggaaaaagtg
    66841 taaacgcatt agtattaaat gatgtttggg gacaacttct taaaaaatat tatggacaaa
    66901 gctatgaatt agaccaatta tctaaggtcg gatgggctag aatcccacat ttttataata
    66961 gtttctatgt ctataaatat gctacaggtt gtagtgcagg agtaagtttt gcccaagaca
    67021 tcctaaagaa tggttcagat aactatatga gctttcttaa aaagggaagt tcagattatc
    67081 caataaactt gttaaaatca agtggcataa atttaactaa tacaaaaccc atagaaaata
    67141 caatcaaaaa atttgatagt ttagtagcag aacttgaaaa gctattataa acattattgt
    67201 aattcacatg gtaaatatat cgaattaatg ataaaatata ctaaaagttt aaagtcctat
    67261 caagatagga ctttttattt attaattagt gggagatgat tctatgcaag ttaaagttaa
    67321 aaaggctgta ataccagcag caggtttagg aactagattt ttgccagcta caaaggcaca
    67381 accaaaagag atgttaccaa tagttgataa gccaactcta caatacataa tagaagaagc
    67441 tgtggcctca ggaatagagg aaatactaat aataacaggt agaaataaaa aatctattga
    67501 agaccacttt gataaatctg tagaattgga acttgattta gagaaaaaag gtaagaaaga
    67561 attgttggag gtagtacaaa acatatctaa catgattaac atacattata taagacaaaa
    67621 agagccaaaa gggttgggag atgcaattta ttgtgcgagg cattttatag gtgatgagcc
    67681 gtttgcagta atgttagggg atgatattgt agataatgat gtaccttgtc taaaacaact
    67741 aacagatgca tatgaggaat atagaactac aatattgggt gttcaaaagg taaatcaaga
    67801 agatactaat aaatatggga taatagaagc aaaaaatata gaaggtagag tttacaaggt
    67861 aaaagacatg gtagaaaagc cagagtcagg aaaagctcct tcaaatattg ctattttagg
    67921 tagatatata ataactcctg aaatctttga tattcttaag gatttaccac caggtaaagg
    67981 tggagaggta caacttacag atgctttaaa aatattatct aagaaagaag ctatgtatgc
    68041 atacaatttt gaaggcaaga gatatgatgt tggggacaag ctaggatttt tagaagctac
    68101 tgttgacttt gctctaaaga aagaagacct taaggaagat tttataaaat atttgaaaca
    68161 tgtatgtagt gaatttgata aaaataacaa tgtgttatac aataatacag atgaaaaaat
    68221 agatttaatt gaagtcaatg aagctagaga acttgaaatt caaaaataat taatgaaagg
    68281 tgatgtatgg tggcaaaatc aagtcagaaa agaattaata gaataatagc tgttgggatt
    68341 gtgggaatcc tcgtaataac gagtataaca gctgttttga gtatgattgc aactatgtaa
    68401 taagataaat gacagcagtt gatatacata aatataaaaa ccatatataa cttgatgtag
    68461 tcaaaaactt acaatatttt agttatatat ggtttttaga atttataaat gtaaaaaaaa
    68521 atttttataa tagttgcata ttttaaagga aaatattatt ataagatata aatatagtga
    68581 taaatttata aaaacgtgct aagattggac ttgaaataca gtaatatagt aggtaataat
    68641 gttttaaatt ttatttattg accataatat ataaataatg atattattag acttaactgt
    68701 ttaaataaaa cttttctttt tcatgccttc aatttccaaa ataagtatgt atataaatga
    68761 atatatagat gaggaggatt tttttgattt tagatgataa agtattagaa ggattaggta
    68821 gtaagacctt agttgagcta cgagagatag ctaaagaatt aaaaataaaa tcaattacta
    68881 catataagaa aaatgagtta attgagatta taaattccaa aagtaaaaat gaggaaaaag
    68941 taaacgaaga gactaaagaa aaaaatgaag atattaaaga agacataaag aatagaaaag
    69001 aaaatataga gcgtgaaagt atagaaaaag aagttgaata taagagccct accaaatcat
    69061 acaacaagaa agaaatagat acccaaaata tttcccaaaa ccaagaacat agaaatcaaa
    69121 gaaatgacta taataagaca acgagtgtaa atgatagtaa taagtcatca aatatatcta
    69181 atagaatggt cagaaataat aataaaaact attatatgcc taaacaagtt gatgaatcta
    69241 agatagtaga tgaatttaac acatcaaaag aggatgaagt agtaggtgta cttgaaattc
    69301 tgccagatgg atttggtttt ttaagaggtt caaattattt gtctactgaa ggagatgttt
    69361 acgtttcacc atctcaaata agaagattta atatgaaaac aggagacaag attaagggaa
    69421 taactagaca tccaaaaagt ggagagaaat ttagagcact tttatatgtt caaaaaataa
    69481 atgatgaaaa ccctgacaca gctatacaaa gaaatgcgtt tgagacatta acaccaatat
    69541 ttccagaaga acgacttaca ctggaaacta atagaaatga aatagctaca agaattatag
    69601 atttaatttc acctatagga aaaggacaaa gaggattgat agtagcacct ccaaaagctg
    69661 ggaaaacagt tcttttaaaa agcgttgcaa atagcatagc taaaaatcat ccaaatgttg
    69721 aacttatagt actccttata gatgaaagac cagaggaagt tactgatatg aaagaatcta
    69781 ttgaaggaga cgttatatat tccacttttg accaagtatc aagtcatcat gtcaaggttg
    69841 cagagatggt gttaaataga gctcaaagat tagttgaaca tggtaaagat gtagtaattt
    69901 tactagatag tataaccaga ttggcaaggg catataacct tacaatatct cctacaggta
    69961 gaactttatc tggaggtatt gaccctggtg cacttcatgg acctaaaaag ttctttggtg
    70021 cagcaaggaa tattagacag ggtggttctt tgacaatatt aggaacagca ttggttgaaa
    70081 ctggttctag gatggatgat gtaatatttg aggaatttaa aggtacagga aatatggagc
    70141 tacatttaga tagaaaatta gcagaaaaaa gaatattccc tgcaatagat atttacaagt
    70201 ctggtacaag aagagatgat ttattgcttg atgatgaaga aaagactgct ctttggagat
    70261 taagaagaga aatgagtaat aattcagtaa tggaaattac agataaagtc attgaactta
    70321 taaagagaac taaggacaat aaagagtttg taaaatcaat aaagaatttg taaaatcaat
    70381 aatacaaatg caaatatgga ttatataaac taatagaaaa atcacttgaa tggcataata
    70441 agttgtgcta taataatgta gttgataatg taaaaagaaa gttaatataa ttaatttttt
    70501 taaataaaga gaagaggtga ctataatgca aaaggaaata caaccaaaat acaatccagt
    70561 tgaagtgcgt tgtgcttgtg ggaacacatt tgttgctggt tcaactaagg acgaaataaa
    70621 agttgaaata tgttcagagt gccatccatt ctatacagga aaacaaaaga acatagaaaa
    70681 aggtggaaga atcgacaaat tcaagaaaag attcaaaatg gactaatttt tataaaagtg
    70741 gagttggagg aagccaactc ctattttatt acaaaaaaac attcatattc tactttaaat
    70801 tgtctttttg tattataata ataaatatgt aaaattgagt attctaaata caataaaatg
    70861 aggtgagtgg atggcaaaac aagctgtagg tgggcaagct gttattgaag gtgtaatgat
    70921 gcagtcaaaa gacaaaagag cggtagctgt tagaaaaagt gacggagaaa tagcactaaa
    70981 agaagataga ataaaaagtt gggtaagaga taagaatata gataagatac cttttgttag
    71041 aggttctttt gttatgatag atacaatgat tcaaggcata aaaagtttaa acttttcttc
    71101 agagtttttt atggaagaag ctgaagagga taaatttgat ttatttataa agaaaatttt
    71161 taaagataag gcaaatgata taattataat attttcattg gtcatagcaa tgttactttc
    71221 tgctggatta ttcattttta tacctacatt ggtaggagga gctttttcta aagtaatgcc
    71281 taatgatttt atgcttaatt taatagaagg aatcataagg atagctatat tatttgcata
    71341 tatagtattg atttcacgaa gtaaggatat agaaagagtc tttcaatatc atggagcaga
    71401 acataaatct atatattgct atgaaaatga tttagaactt acagtagaga atgctagaaa
    71461 atttaaaaga ttacatccta gatgtggtac aaatttccta tttatagtta tggctgtatc
    71521 aataatattg tttgcatttt ttggttggcc aaatccaatt cttagaatat ttatgagaat
    71581 tatatgtgtg cctatagtag caggtttatc ttatgaggta attagagttc ttgggaaata
    71641 tgataatgga tttactaaaa ttattgcata tccaggaatg atgttgcaat attttacaac
    71701 aaaagagcct gatgatgagc aattagaggt tgctttggaa gcattgaaag cagtagtaga
    71761 ttagagaaaa ggatatttgt tatgacgata aaagatataa ttattaaata ttcagataaa
    71821 ctaaaagata taagtgatac tccaagatta gatacagagt tattgttaca aaaaacattg
    71881 ggtgttgata gattatatat tcacttaaat ctgaataaag agctaactga agaacaaaaa
    71941 acaaaattta tgggttttgc agaagaaaga ttaaatggaa gaccaatagc atatatagtt
    72001 gaaaataggg agtttatggg attagatttt tttgttaagg aaggcgtatt gattccaaga
    72061 ccagatacag aaactttagt agaagaaata attgaaatat gtagagaaaa aaaagatgta
    72121 agtatattag atataggaac tggttctgga gctattacaa taagtttagc aaagtacatt
    72181 gaaaattcta aaatcatgtc ttttgatata tcagagacag cattagaaat agctaaaaaa
    72241 aatgctatta taaatgaagt tggtgaaaaa ataaagtata ttaattctga tttatttaca
    72301 gcaataagtg atagtaacat aaaattcgat atcatagttt caaatccacc atatattaaa
    72361 aaacaagata tagaaactct gcacaaacaa gttaaagatt atgaaccata taatgctctt
    72421 gaaggtggag aagatggatt agacttttat agaagaataa cagaacaagg taagaaatat
    72481 ttaaataagt gtggaatatt agcatatgaa gttggtcata atcaagctga agatgtaata
    72541 aatattatga aaagtaatgg atataaaaaa atatacacaa agaaggatat tcaaggcatt
    72601 gatagagttg ttataggtta caatatatga tataatcaac aattgatatg ttacatttaa
    72661 tcgaggtgaa agtatgttaa aaaaattaga ggtattggaa gatacatata aagacttaag
    72721 tgaaaaaata ggtgatccgg atgtaataaa tgatcaaaaa gtttggcaga aatatataaa
    72781 agaacatgct gacttggaac ctattgttat gaaatacaga gagtataaaa gcgttttaga
    72841 tagtataaaa gaatcaaaag aaattttaca agaagagtca gatgaagaat taagagaatt
    72901 agctaaaatg gagttagctg agatggaaga aaaagtagca cctctggagg aagaaataaa
    72961 aatactatta ttgcctaaag accctaatga tgataagaac gttatagttg aaattagagg
    73021 tggagctgga ggagatgaag cagctctatt tgctggtgat ttatttagaa tgtactcaag
    73081 gtatgctgaa agaagaagat ggaaaataga attattgagt gcaagtgata ctggagttgg
    73141 tggatataaa gaagtatctt ttatgataaa aggtaaaggt gcttattcaa gattaaagta
    73201 tgaatctgga gttcatagag ttcaaagaat accatcaact gaatctggtg gaagaataca
    73261 cacatcaact tctactgttg cagtattacc agaagttgaa gatgtagagg ttgaaataaa
    73321 tccaaatgat ttaagaatag atgttttccg ttcttcaggt aatggaggac aaagtgtcaa
    73381 tactactgac tctgctgtta gggtaacaca tatacctaca ggtgaagtag tatcttgtca
    73441 agatggaaaa tcacaattaa aaaataaaga gcaagcttta aaaatattaa aagctagact
    73501 ttacgataaa gcattggcag aacaacacaa agatatagca gcagaaagaa aaagccaagt
    73561 cggaacagga gacaggtcag aaagaataag aacatataat ttccctcaag gtagaataag
    73621 tgaccataga ataaatttaa ctttatataa gttagatgca ttcttagatg gagatataga
    73681 tgaaatgata gatgctttaa ttactgttga ccaaacagaa aaaatgactg caatataagt
    73741 aatgttttaa agaagctgtt tcttcctaga aattattttc tattttgaaa cagctatttt
    73801 gcattttagg tcatgttatc atattctatt gtatataatt gttatataca agtataggag
    73861 ggttatatga tactaaaagt aacaataatt gggcttttgg caggtgttat agggacagga
    73921 ttaggaggag taatatcagc catttttaaa agagaggtcg ataaatatct aagttttttt
    73981 atgggattat ctggtggaat aatgttggct gtagtggtgt ttgatttaat gaaagaatcc
    74041 atggacaaaa tggggataat caatacagtt atatttacgt ttgttggagc tctcataact
    74101 atgtacataa aaacaaaatt agatgtgtct ggaaatatgg catctggata tcttatattc
    74161 ataagtatat tactacataa tttaccagaa ggactagcca ttggttcttc ttttatgtca
    74221 actgaaagtt taggtattac acttgctata gttataggtc tacataatat accagaaggg
    74281 ttagctatgg cattaggctt agtttgcaat aaaatgaaac taagtaaagt tattttgttt
    74341 actgtaatag ctggattacc tatgggtcta ggaagttttt taggtgttta ttttggagga
    74401 gtatttactt ctcttatagg agtatttctt gctacagcag gaggtactat gatgtatgtt
    74461 gtattggaag aaatttttcc acattcaaaa agtgtgtatt gtataattgg tttcttgtta
    74521 ggtacaatga ttgtaaatta tatataaaat taaaatgaaa gcaggcatac aaaagatgaa
    74581 aactattata agtaatattg atataaataa tattgattat gaggaaataa aaatacaagc
    74641 aaaacttctt agagaaggta aaacggttat ttttccaact gagacagtat atgggttggg
    74701 tgcaaatgca ttagatgaaa atgcagtaaa gaagatttat gaagctaaag gaagacctag
    74761 tgataatcca ttgatagtcc atatatatga aaaagaagaa gtatatgact tagccaaaga
    74821 tataagtgat aaagctaaac ttgttataga aaagctttgg ccaggaccta taacaattat
    74881 attaaataaa aaagatataa ttccatacaa aactagtgga gggcttgata cagttgcaat
    74941 aaggatgcct tctaatgtta ttgccagggc aataataaaa gaagctggta tacctattgc
    75001 agctccttct gcaaatatct caggtcgccc atctccaaca aaagcaaaac atgtatatga
    75061 agagatgaat ggaagagtgg atggaatagt tttaggagga gacagtaatt ttggattgga
    75121 gtcaactgta ttggatttaa cagaagagac tcctatgata ctgagaccag gaagtataac
    75181 taaagaagtt ttagaggaac ttcttggaga agtaaagctt gacccatcat taagtaaaaa
    75241 agaagataat caaaaagcta aagctcctgg aatgaaatat aaacattatt ctccaaatgc
    75301 agatgtatat ataatatctg gaaaaagaga aaatgtagca acaaagatta atgatatgat
    75361 aaaatctaat agagacaaag gtctaaagac aggtgtaatg tgtattagta gaaaccgaga
    75421 gttttatgat ggagaagtaa tcgatttagg aaactctctt gaagaagttg caagtaatct
    75481 gtttgatgct cttatagaaa tggacaaaaa gggtgttgat gtcatatatt cagaagagtt
    75541 ccccaagact ggagttggac aagcaattat gaatagacta ctgaagtcag caggatacaa
    75601 agtgataaag gcttaagagg gagttgtaaa atatgaatat acttattgtg tgtacaggta
    75661 atacttgtag aagccctatg gctgaggcta tactaaggaa agcaattaaa gaatctggaa
    75721 gaagtattga agaatattct atatcatcag caggtatatc tacagcaaat ggaatgggtg
    75781 cttctgaaaa ttctatagaa gtactaaaag aaatcggtat agacttatca aatcatagaa
    75841 gtaaagtaat tactaaaaaa ttaattgatg aatcagatat aatactaaca atgactaaat
    75901 ctcataaaga aattttagta caagcagttc caaaatgcaa agaaaaagtg tatactttta
    75961 aagggtttgc aaataaaaat gaggaagata tatctgaccc atttggtgga aatttggata
    76021 tttatagaag cactatgaga gaaataatgt attctgtcaa cgaaatagta aaaaaaatat
    76081 gaaacggagg ataaacaaat gaagatagga ttaggctgtg accatggagg atataattta
    76141 aaaaaagaaa taataagcta tttagaaagt aaaggtatag agtgtgtaga ttatggaact
    76201 aataatgcta cagattctgt tgattatcct gtttatggag aaatagttgc aaattcagtt
    76261 ataaataaag aagtagatta tggaatatta tgttgtggta cagggatagg aatatcttta
    76321 gctgctaata aggttcctgg gataagatgt gcagttgtat cagatgtatt ttctgcaaaa
    76381 atgtcaaaag cacataatga tgcaaatatg ttatcattag gcgaaagagt tttaggaaaa
    76441 ggtctagcac ttgagatagt tgaagcatgg attaatacgg attttgaagg tgatagacat
    76501 gctagaagag taaacatgat taaatcaata gaagaaaaac ataataaata ataaaaatta
    76561 cagttttatc aggaggaatt atcaaaatga gcaaagtggt agagacaaac catccattaa
    76621 tacaacataa attaacttta atgagagata aaaatactgg ttctaaagac tttagagagc
    76681 ttttaactga aattgcaatg ttaatggggt atgaaataac aaaagatata ccattaaaag
    76741 atgtagaaat agaaactcct attcaaaaaa catcatctaa agtagttgca ggtaaaaaat
    76801 tagcaataat tccaatttta agagcaggtc ttggaatggt agatggatta gtgagcttaa
    76861 tgccagcagc aaaagttgga catgtaggac tatatagaga cccagaaaca ttaaaaccag
    76921 ttgaatatta ttgtaagctt cctcaagaca taggagaaag agatataata gtagttgacc
    76981 ctatgcttgc aacagggggt tctgctgtag cagctataga tttacttaag tctaaaggag
    77041 ctaaaagtat aaaattagct aatttagtag ctgcaccaga aggtatagca gaagtgcaaa
    77101 aatatcatga tgatgttgat atatatgttg catcagttga cgaaagatta aatgaacatg
    77161 gatatataat tcctggtctt ggtgatgctg gagatagatt gtttggaact aaataaaaat
    77221 aaataactat aaatatttat tagggacttt cttatatagt gtatataatt ttatgtaaga
    77281 aggttctttt ttatatattt tttctcaaaa taggaattat tgaattgata aatttctcga
    77341 attgagaata aaaagttttt accaattaaa aaagattgct tattttacaa ggttttatag
    77401 aatagtagtc tattttaatg tggcatgtta gttgcttacg attataggta ttaagtgtat
    77461 tagttaaatt gagaattgaa tattaataat attattggga gtggtaatac atgaagaatt
    77521 gtatagtagt aagaggggct ggagatttag caacagcagt tattcataaa ttacacaaca
    77581 gtggttttaa agtagttgcg ttagaaatag aagagccatt agctataaga aggcaaattt
    77641 ctttttgtga ggctgtttat gaaaaaaaag taactgttga aggtgtagaa tgtatatttt
    77701 gcaatactga aaaagaaata gaggaagttt tatctcaaca taaagtagcc ttgattatag
    77761 acccaaaagg agaaaaatta tctaaattaa atccaatcgt tttggttgat ggaatactcg
    77821 ctaagaaaaa cttaggtaca actaaagata tggcaccact tacaatagct ttaggtcctg
    77881 gattttgtgc aggagaagat gtagatgttg ttatagagac aatgagaggt cataatctag
    77941 gaagaataat aactaatggt agagcagaaa aaaatacagg agtaccagga gcaataaaag
    78001 gtttttctaa agagagagtt atgcattcac agtatgatgg cataatggaa aatgtatgta
    78061 agataggtga tatagttgaa aagggagacc tactttgcta tattaataat tatgaaaaaa
    78121 aacatgaagt tagagcaaca atatctggag tccttagagg tttattaaag gatggagcaa
    78181 aggtaaacaa aaatttaaaa atactagaca tagaccctag aaaagaagaa gttaagaatg
    78241 cttttacaat atcagataaa gctagatgta tagctggagg ggttttagaa gccatactaa
    78301 acaatgggat ttatccccaa aattaattta tattagataa agctattaat attttattaa
    78361 gatgatataa taaatttaat agattaaaga gagcatatga cgggtattta aaatttaaat
    78421 ggtaaaaatg ctcttttttt attggacatt caattaacat gtgtatataa tataaaaata
    78481 agtatttatt taggttgaaa tctaataaat atataatacc agtacataag caaagaggag
    78541 aaaagtaaat tatgagacca tcatgggatg aatacttcat ggaaattgca gaagtagtga
    78601 aaaagcgttc aacttgtatt agaagacaag taggagctgt aattgtaaga gacaaacaaa
    78661 tattaacaac tggatataat ggttctccga gaaatcttga acactgtgaa aatattggat
    78721 gtaagaggca ggaattaaat attccctcag gagaaaggca tgaattatgt agagcattac
    78781 atgctgaaca aaatgcgata atacaagctg ctcataatgg tattagtgta gatggggcaa
    78841 ctttgtatgt tactacaaga ccttgtgttt tgtgtgcaaa aatgtgcata aatgcaggta
    78901 tattgaaaat tgtttatgaa ggtgattatc cagatgatat gtctacagaa ttattaaaag
    78961 aagcaggaat agaactcata aaattttagt ggataaaatt tatatgacaa taaatgtcaa
    79021 attatgacac tgaaatacaa tggtatcaaa tgattaaaag aaagtatttg aaaaatcatc
    79081 agattagtag taagatatat tttataatta aataaattta tgagatagat taattacatg
    79141 ttatttattt tataataatg agggatacta tttatgagta aaagaaatac acatacagaa
    79201 attgcaaagt tgtttagttt aataagtcaa ataggattga tgattgtaat atcaatatta
    79261 ggatgtactt tcttaggaaa atttttagat agtaaattga atactactcc agttctgaca
    79321 ataatatttt tattgttggg tgttggaggt gcttttagtg gtgtttataa aactctaata
    79381 gtatatacac gaaggaagtg atatattgga tataaaatta cttaaccagg taaggtcagt
    79441 aacaaaaggt atagtaatat ttgatgtagt tttaattgtg ctaatgttga taacatcaac
    79501 attaagtaaa ccagtattat taggacttat atttggctct ataatagcta tgttaaattt
    79561 tagattattg gcgatatcac tggaaaaatc agtgacattt cctgctggga aggcacaagc
    79621 ttatgcatca gcacaatatg ctgttagatt atttataatg gcagttgttc taattgcatc
    79681 agctacagta ccacatttaa atataatcgg aacagcattg ggattattga gcacaaagtt
    79741 tgtcatatta tcaaaaaact ttataaataa attaaagagg aaggaggcat aagtatgaat
    79801 caagcgcatt attatctcat aggaaacttt aagattagtg aaacaatact agtaagttgg
    79861 ggaataatgg cctttttagc gatagcatct tattttttga ctagaaactt aaaaaaagtt
    79921 ccaactagta aagttcaaat attcttagaa tttgcagtag gtggtcttgc taaaatggtt
    79981 acagatgtta tgggtaaaaa tacagtaaaa agaatgccat atatgatacc atacatagga
    80041 agcttgtttt tatttttcgc atgttcaaat ttaagcccat tacttggatt aaggtcacca
    80101 acaaccgact tagacacaac tgctgcatgg gcaatgatta cattttttat gatttatttt
    80161 gcaggagtta aatttaaggg gttatcatac tttaaagaat tggctcaacc acttgccata
    80221 atgttaccaa tcaatattat aggtgagtta gctagaccaa tatcattaag tttccgtcca
    80281 tttgggaata ttttaggtgg aacaataata atgggtctgt tttatcaact tttaggtttt
    80341 atatctagtt taataccagg attatcaata ccaattggag aaatattaat accagtccca
    80401 ttacacttgt actttgactt atttgcagga ttgttacaag catatatatt tataatgtta
    80461 acaatggtat ttatatcaag tgcaggagag ttagattaag aaatcaactt atattaagat
    80521 aaaaataaaa taatttaaaa aattaaagtt ttaaggagga aataattatg gaaacagcaa
    80581 tagtagcggc agcatcagca ataggagcag ggatagcagt tgcaacaggt ataggtgcag
    80641 gtataggtca aggtatagca gcagctaaag ctgcagaagc agtaggaaac caaccagaag
    80701 ctaaaggtga tataacatca actttattac taggagttgc tatagcagag tcttcagcaa
    80761 tatatggttt ggttatatca ataatacttc tattcgtaaa tccattcttt aaatacttag
    80821 gaatgtagat ttagtcctta aatattttga gataggttta taggtaaatc atctcaaact
    80881 tataatcatg agatgaactt tatcatctcg tgattataag tagttatgat ttatggaaag
    80941 gaggataatt atggaaaaag cactagtagg tataacgtgg gaatttgtgt tccaaattgt
    81001 taatactttt ataatatttt tacttctgag aaagctttta ttcaagccag tattaaatat
    81061 aattgaatca agagaaaatg atattaaaag tgatttggca gaaggcgaaa aagcaaaaaa
    81121 tgaaggttta gctctgaaaa aagaatatga gtcaaaaatc aattttgcca aggatgaagg
    81181 tcaagaaatc ataaaacaag caactataag agcagagcag aaatcagatg atatagtaaa
    81241 tactgcaaag aaagatgctc ttgatataaa agaaaaagca aacaaagata tagaacaaga
    81301 aagacaaaag gttataaatg aaatcaaaaa tgatatatct aacatagcac tacttgcagc
    81361 atctaaagtc attgaaaaag atttagataa atctaaacat gaagaattga tagaaaactt
    81421 tataaaagag gtaggggaag ctaaatgata aatgtaatag caaatagata tgcagaagcc
    81481 ctatttcaat taggagaaga agaaaattca actgatgtct tattcaaaga actagaaaaa
    81541 gtagtagata tgatgactaa agttagtaaa gatttctaca aagttttaaa atcaccatta
    81601 gtttcaaaat cagaaaagaa aaacttggta gaaattattt ttagtaaaga agtaagttct
    81661 aacataaaaa actttttaaa agttttagta gataaagata gaatatcata tttagaagat
    81721 atcgaattag cttataaaga attattaaat aagaaaaaca atgtaattga tggagtagca
    81781 ataagtgcta ttccaatgag tgaaacagat attaaagaac ttgaagtcaa actttcaaat
    81841 aaatacaaca aaaatgttac tatagaaaat gttgttgaca agactatact agggggagtt
    81901 ctagttagaa ttggaaatga acaaatagat ggcactgtga aaactcgtct tgataagatg
    81961 aaagaaaagc tatctgaagt aatatcttaa gaatggcggt gaacccatga acttaaaacc
    82021 tgaagaaata agttctataa ttaaacagca aataaaaaat tatgagaata aagttgagtt
    82081 gacagataca ggtagtgttt taacagttgg ggatggtata gcgagtgtat atggattaga
    82141 aaaagctatg tctggtgagt tgctagagtt cccaggtgaa atatatggta tggcacttaa
    82201 cttggaagaa gaagtagttg gagctgttat ccttggagat gacagtgaaa taaaagaagg
    82261 cgatatagtt aagagaactg gaagaatagt tgaagttcca gttggagaag ctttaatagg
    82321 tagagttgta aactcactag gtcagccaat agatggaaaa ggacctatag cgtacacaaa
    82381 aactagacca gttgaatctg aggcacctgg tataatagat agaaggtctg tttatgaacc
    82441 attacaaaca ggaatcaaat caatagattc aatgatacca attggtagag gtcaaagaga
    82501 gttaataata ggtgacagac aaactggtaa aacatctata gttattgaca ctatactaaa
    82561 tcaaaaagga aaagatgtta tctgtatata tgtagcaatt ggacaaaaac gttctactat
    82621 agctcaatta gttagctctt tagaaaaagg tggagctttg gattatacaa tagtagtttc
    82681 agcaacagca tctgaatcag caccacttca atatatagca ccatatgctg gagcagctat
    82741 gggtgaagaa tttatgtaca atgggaaaca tgtacttata gtgtatgatg atttaagtaa
    82801 acaagctgtt gcatatagag aaatgtcatt gttacttaga agaccaccag gacgtgaggc
    82861 atatcctgga gatgtattct atttacattc aagattactt gaaagagcag ctaagttatc
    82921 tgatgaattg ggtggaggtt caatgactgc cttgcctata atagaaactc aagctggtga
    82981 cgtttctgca tatataccaa caaatgttat atctataaca gatggtcaaa tatacttaca
    83041 accagagtta ttctattcag gtgtaagacc agcagttgac cctggtatat cagtatcaag
    83101 ggttggtggt tctgcgcaaa ttaaagcaat gaaaaaagtt gcaggaacat taaaacttgc
    83161 atattcacaa tatagagaac ttgcagcatt ctcacagttt ggttctgact tagatgaaga
    83221 tactaaaaag agacttgctc aaggtgaaag aatcgttgag atattaaaac aaggtgagca
    83281 tcaaccaata aaagttgaaa atcaagttat gataatatat gctgttataa ataatcattt
    83341 agaggatatt ccgatagata atatagctag atttgaatca gaattgtatg catttgtaga
    83401 taataattat ccggaaatat caagaaaaat attaggtgga gaagacttca ctcatgattt
    83461 aacagatgca ataaatgaat ttaaagaaaa gtttgttgtt gaagtataat cctttttaaa
    83521 aaaggaggta aattaattgg caggagctgg aatcaaagca atcaaaacac gtatgggaac
    83581 tgttgaaagt acaaagcaaa taactaaagc aatggaatta gttgcatctt ctaagcttag
    83641 aaaagcaaaa gaaaaggctg aagattcaag agaatatttt aatactttat acaatacagt
    83701 aaaagatatt gcccaaacaa cacgtggaat tagaaatgaa tttttaaaag aaagagaagt
    83761 aaaaaataaa tgctatgtag tagtagctgg agacagagga cttgcaggtg gatacaattc
    83821 taatgtatta aaggaatctg tctctcatat gaaaggtaaa aaggaaacag tgattactgt
    83881 tggaaagaaa gcacatgaat ttttttctaa aagaaactac aatatagcaa aagatataaa
    83941 tagtgttgaa gcttgtggat atgaagatgc gacagaaatt tcacaaacta ttattgacct
    84001 gtataaaaaa ggtgaagtag atgaagttta tattgcttat acaaaattta aatctgcttt
    84061 atcacaagaa gttgaaattt tgaaattgct tccattagtc tttgaaaaag atacagacga
    84121 acaaggaatg gcagtaaaag aagaaaaaca aaatagatca aaagtacaat atataccatc
    84181 agcagaagca gttttaagtt atattgtacc tcagtttata tcaggtacag tatatggtgg
    84241 aattttggaa tcttttgcat cagagcaagg tgctagaaga actgcaatgg aatcagccac
    84301 agacaatgcg aatgaaatgc tatctaattt agagttaagt tataacagag caagacagtc
    84361 agctgtaact caagaaataa cagagatagt tggtggagtt gaagctctaa aatagggagg
    84421 ttaggaaatg gcaaatgtag gtaaagtagt acagattgta ggagctgtac tagatgttaa
    84481 gtttgatagt gaacaaagcc tacctaactt attaaatgcg ttagtaataa agttaggaga
    84541 taaagaaata gttgcagaag ttgctcaaca tataggtgat gatactgtaa gatgtatagc
    84601 tatgagtgca acagacggac ttgtaagagg tatggaagtt gtagatacag gagggccaat
    84661 aagtgttcct gttggagatg aaactttagg tagaatattc aatgttcttg gaaaaccagt
    84721 tgatgggaaa ccagctccaa aatctgcacc taaattacct atacatagac cagcaccagc
    84781 atatgatgaa ctagaaacta cagcagagat actagaaact ggtattaagg tagttgactt
    84841 actagctcca tatctaaaag gtggaaaaat aggtctattt ggaggagcag gagtaggaaa
    84901 gacagttcta atacaagagc ttataaacaa tattgctaag caacatggtg gtatttctgt
    84961 gttttcagga gtaggagaaa gaacaagaga aggtaacgac ctttatggcg aaatgagtga
    85021 gtctggagtt ataaataaaa cagctctagt atttggtcaa atgaatgaac cacctggagc
    85081 gagaatgaga gttgctttaa ctggacttac aatggcagaa cattttagag atgagcaagg
    85141 acaagacgtt ttacttttcg ttgataatat attccgtttc acacaagctg gttcagaagt
    85201 ttcagcactt ctaggacgta tgccatcagc tgttggttat cagccaacat tagctactga
    85261 aatgggtgca cttcaagaga gaataacatc aactaagaaa ggttcaataa catctgttca
    85321 agcagtatat gtaccagcag atgacttaac tgacccagca ccagctacaa cattctctca
    85381 cttagatgca aaaacagttt tatcaagaca aatatcatca ctgggtatat atccagcagt
    85441 tgaccctctt gaatctacat caaggattct tgaccctagt atagtaggaa aagaacatta
    85501 tgaagtagct agaggtgtac aatcaatact tcaaagatat aaagaacttc aagatataat
    85561 tgctatactt ggtatggatg aattatctga tgaagataag ttaatagttg cacgtgctag
    85621 aaagatacaa agattcttat ctcaatcatt cacagttgct gagcaattta caggaaatcc
    85681 aggtcaatat gttcctgtta aagagactgt aagaggattt aaagaaattc ttgaagggaa
    85741 acatgatgac ttaccagagt cagcattctt atttgtagga acaatagaag acgctgttag
    85801 aaaagcgaag ggaagtatgt agtcatggcc agtgaatttt cattgaaagt tgtaactcca
    85861 agtaatactt tttatgatgg agatgttgaa atgatagtag ttagaactac aaaaggagac
    85921 aaaggggtgt tgaaaggtca tataccattt gtttcaggta tacctgtagg aactttaaag
    85981 ataaaaaaag agggaagttt taaagaggcg aaaatagcag aaggattcat taatgtaact
    86041 caagataaaa ctgtcatatt gacagaatct gctgagtgga tttaataaat taatttagag
    86101 tataaaaagt cattttgacc aaaaaaactt ttgggtaaaa tggctttttt gcgttttggt
    86161 gttaataaaa taacgataaa attgtaagtt tttagtgata ataaattcac aaaatgttta
    86221 aatttactgc caaattatgt tgtaattaat aagataaaga agatttagat agcttttatg
    86281 taagtgaaaa agtcttcata taagaaatac atataaaatt tttataaaat ttaaaaaatt
    86341 tgcttagttg tgtagtataa taaataatat aatagatttg tatataatat tcattacaaa
    86401 taattttaaa agttacagtt ttattataat gattattaaa ttaagataag taacataaac
    86461 taaataataa tgtgtattat cgatagtaaa tttataaatt cattattaat tattgaaaga
    86521 agtattataa attttctgga ggaacaatga atatatttga tattggcgta gatataattg
    86581 aaattgatag aataagaaag gctgtagaca aaaataatag gtttttggag aagatattta
    86641 cagatagaga aatagagtat tttaattcta agaattttaa agcagaaagt atagcaggta
    86701 atttcgcagc caaggaagct ataagtaaat ctataggtac aggaattaga ttgtttaatt
    86761 ttaaagatat agaagttctc agaaatgaaa tgggaaaacc aatagtaaaa acttataata
    86821 atctagcaaa aatgtgcata gattataatg ttttagaaat aaaagtttca atatcacatt
    86881 caaaagatta tgctatagca aatgcgataa ccataattaa ggactaagga gtgattacat
    86941 tatgatggat aaaacagcaa aagaaataat gacaacagat gtaatagtag caaaacaaga
    87001 tgatagtata gcagatgtag ctaatatgct aattgcagaa aaaataggag gtctgccagt
    87061 tgtagattca gagaatagag ttgttggaat aatttctgaa acagatattc ttaaaaaaga
    87121 aaaatatata gaagcacctc tctatataaa tctattacaa ggattgattt tcttagatga
    87181 cttaaaaaaa gttgaaaaag atataaaaca agtagctgct tataaggttg gagaacttat
    87241 gtctaaagat ataataaagg ttcatgaaga tgataagttt gatgatgtag ctaatgtaat
    87301 gattaaaaaa tcaataaaca gagttcctgt agtagatgat gacaacaaac ttaaaggtat
    87361 aatttgtaga tatgatataa taaaagcttt atacaatgaa taaactgaag taggaggtgt
    87421 gatgtgagaa aaaagtggac catagtatgt attatgttct tggcactggt tataattgta
    87481 ataggatgcc aaaaacgaca gtccacaaaa gaagaagtat acaaagattt tcaaaagcag
    87541 atttctgata tgaattatta cagttgtaaa gctgaagtag aagtagttgg aaataaaagc
    87601 ccacacaatt atgttttaat tcatacttat aaaaaaaccg ataactataa attagaagta
    87661 atttccccaa aacatttaaa agggaaaagt atagaatatc aagatgataa aatattagtt
    87721 aaaaatccaa agattagtga tgtagtggag ttacctaata caggaaaaaa taatcaatat
    87781 ctttttgtag gagactttat aaaaaactat cttcaaaatg aggagatgaa agtaaagtta
    87841 tccaaaggac atttggtttt agagactttt attcctgggg ataataaata ttttaacaag
    87901 caagtattat atgtaaatgc tgacacaaaa aatcctgaaa aaatggaagt gttagataaa
    87961 gagggagtgc caagatttac agtaaaatac aaagattttg aatacagaaa ctaaggggga
    88021 ctaaagacat gcaaaaaata acagtgccta catgggcaga gataaatcta gataacttaa
    88081 gatttaactt aaataatatt aaaaatttat tagaagaaga tattaagatt tgtggagtaa
    88141 taaaagctga tgcatatgga catggtgcag tagaagttgc aaaattgcta gaaaaagaaa
    88201 aagtagatta cttagcagta gcaagaactg ctgaaggaat tgaacttaga caaaatggca
    88261 taacacttcc tattttgaac ttgggatata ctccagacga agcttttgaa gattctataa
    88321 aaaataaaat aactatgaca gtttattctt tagaaacagc acaaaagata aatgaaattg
    88381 caaaatcttt aggagaaaaa gcctgtgttc atgttaaaat agactcaggg atgactagaa
    88441 taggtttcca acctaatgag aagtcagtac aggaaataat agaattaaat aaattagaat
    88501 atatagattt agaaggtatg tttactcatt ttgctacagc tgatgaagta agtaaagagt
    88561 acacttataa acaagctaat aattataaat ttatgtctga taaattagat gaggctggtg
    88621 taaaaatagc tataaaacat gtatcaaaca gtgcagctat tatggattgc cctgatttaa
    88681 gattaaatat ggtaagagca ggaataatat tatatggtca ttatccatct gatgatgtat
    88741 ttaaagatag attagaatta agaccagcca tgaaattaaa atcaaaaatc ggacatataa
    88801 aacaagttga accaggtgta ggaataagtt atggactaaa atacacaact acaggtaaag
    88861 aaacaatagc tacagttcca ataggatacg cagatggatt tactagaatc caaaaaaatc
    88921 caaaggttct tattaaggga gaagtgtttg atgtagttgg tagaatatgt atggatcaaa
    88981 taatggttag aattgacaaa gatatagaca taaaagttgg agatgaggtt atactatttg
    89041 gagaaggcga agttacagct gagcgtatag ctaaagactt aggaactata aactatgaag
    89101 tgttatgtat gatatcaaga agagttgacc gtgtttatat ggaaaataat gagcttgtac
    89161 aaataaacag ttatttgcta aaataaaacc atgatattat aatctgggag gcgattttgt
    89221 gtacgataag agtaaagaaa aaatagaagt tactttacct gactctctga tttctgaagt
    89281 tgatagtata gttcagatgg agaacagtaa cagagaagat tttgcaaaag ctgcttttca
    89341 gttctatata actcagaaaa agaaaattca tattaaagaa tctatgaaaa cgggttatag
    89401 agaaatgggt caaattaatc tgtcactagc tgaattaggt atgacagttg aagaagtatc
    89461 ttctgatgat aaaaatgaag ggaagattgc ccaaggtgag tactaattgt gaaataaagc
    89521 gtggtgattt atattacgct gatttaagtc cagttgttgg ttcagagcag ggaggcgtta
    89581 gacctgttct gataatacaa aatgatgtag gtaataaata tagtcctaca gttatagtat
    89641 cagctataac atctcagatt aataaagcca aattacctac tcatgtagag ataagttcca
    89701 atgaatatgg gcttaataaa gactcagtaa ttcttttaga gcaaattaga actatcgata
    89761 aaaagagact gagggaaaaa attggttgtc tagatgaaaa tatgatgata aaagtagatg
    89821 atggacttca aataagttta ggattgttta ctttttaaag agggatttgt cttttattag
    89881 acaatccctt ttttctttta aatatctctt aaggtttaat tttaatgtac tatgttaata
    89941 aaattaaaaa taacgcaaaa aaatagcata aaagtgatat aataatatgg aattgatata
    90001 aaattacgaa ctcaagggag gtaattgcat gagagaccat aaaggattaa atgagattgc
    90061 gcgtatcata agaagagata tagtttcaat gatacataga gccaaatctg gacatccagg
    90121 agggtcatta tcagtagttg aaatacttac tgcgttatac tttgatgaaa tgaatgtgga
    90181 ttcttcaaat cctaaaatgg aggatagaga tagatttgta ttatcaaaag gacatgctgc
    90241 accagcatta tatgctacat tggctgaaaa aggctatttt gataaggaag aactgaatgg
    90301 attaaggaag ataggaagga tgttacaagg tcatccagac atgaaaggca cgccaggtgt
    90361 ggaaatatct actggttctt taggtcaagg tttttcagtt gcatgtggta tggctatggc
    90421 atcaaagttg gataatgctc cgtggaatgt gtatactttg cttggtgatg gtgaggttca
    90481 agaaggaatt gtttgggaag ctgctatgag tgctgcacat tataaacttg ataatttaat
    90541 tgctttttta gataataatg gacttcaaat agatggagat atagaaagcg taatgagttt
    90601 aggttcaata gttgataaat ttaaagcttt tggttggaat gtaattgaga tagatggaca
    90661 tgattttgac caaatatttg cggctcttga tatagctaaa tcaacagtta aaaaacctac
    90721 aatgatagtt gcaaaaacta taaaaggcaa aggaatatct tttatggaaa atcaagcagg
    90781 atggcatggg acagctccaa gtgatgagga gctagaaaaa gctttgcttg aattaggagg
    90841 tgctgacaat gagtaaaatg gcgacgagag aagcatatgg taaagcatta gtaaaattag
    90901 gtcaaataaa tgacaatgtt gtagtattag atgcagattt atcaaagtct acaaaaacac
    90961 atgattttta caagtcattt ccagatagat tttttaatat gggtatagct gaacaaaatt
    91021 taataggcgc tgcttgtgga ttatcaactg ctggtaaaat accttttgca agtacttttg
    91081 ctatgtttgc tacgggaagg gcttttgaga ttataagaaa ttcagtttgt tatccaaagc
    91141 taaatgtaaa aatatgtgct actcatgcag gtcttactgt tggagaggat ggagcatctc
    91201 atgaaagtgt ggaagatatt gctataatga gagctatacc aaatatgaca gttcttgttc
    91261 cagctgatgg agttgaaaca gaaaaaatta tttttgaaat agctaagtat aatggacctg
    91321 tttatgttag attagggaga agttcagtac cagttttatt tgatgaagat tataaatttg
    91381 aaataggaaa aggtacagtc ttaagagaag gaaaggatgt atcaattata gcttgtggaa
    91441 ttatggtaaa tgaagctcta ttagcacaag aaaagttgca agaagaaggt ataagtgcta
    91501 gagttataaa catgtcctcg ataaaaccta tagacaaaga tttaatatta gaatcagcaa
    91561 aagaaacgaa tgctattgta actgtagaag aacatagcat aattggggga ttaggttctg
    91621 ctgtaagtga ggtagtagga gagtcttgtc caaccattgt aaagaaagtt ggaattaaag
    91681 atacatttgg agaatctggt actccaaatg aattgttaaa aaaatatgaa ttaacttgtg
    91741 atgacataat aaagactgtt aaagaagtta ttattgcaaa aagaatgtaa gagatataaa
    91801 ttattcatgt ttgttaataa tataatatat ttaaaagaga ggtataaagt gttatattaa
    91861 tttatatctc tctttttttt atatgatatc aatatatcaa aaatgtaata aaatattcaa
    91921 atttttacaa aaatagagaa aatattcaaa tatatattga ctattcagaa aaatatgact
    91981 aatatataat ttatagagaa actaatttta tatataatga ctacttagat aaaagaatct
    92041 aaaaaaatta tgagaggagg agtagggtat aaaatcagat tataagtttt aagttattat
    92101 actaaaaagc tataaaacag ttatcatgta ttttatgtta aaggaaattg taggtagttt
    92161 atcgaattgt ttaatcagaa gaaaaaattt cacatgggga ggtaactgag atatgtcaga
    92221 aaaagtaaat gttaaaacag tcatcagttt cgcaggtgcg tatgtagcaa cagtaatcgg
    92281 ttctggattt gcaacaggac aggaaataat gcaattcttc tctttttatg gttttgcagg
    92341 tattattggt gggataattt caatggtatt attctcatgg atgggtgctt cagttatgtc
    92401 aaaaggtaaa gagcttcaac ttaaagaacc tatcaaaatt tatcgtgttt attgtggtaa
    92461 gtatttagga atattctttg aatggtttgg acctttattc ttatttggtg ttttcgtagt
    92521 aatgatttca ggagcaggag caactttaac tgaatactat ggattaaatc catttgtagg
    92581 tagagtaggt atggcagtag tagcattatt aactgtttca ctaggtcttg ataaattatc
    92641 aaagatacta ggtggaatag ggcctataat aataatcttt acacttttag ttggtggtat
    92701 aagtttagct aaaaacatag gtaatatagg agaagctgca caagttctta gcactgtaaa
    92761 gatagctaaa ccagttccaa atgcctatct atctggtgta atatatacta cttataacac
    92821 aattgttgtt atggcattct taactggatt aggagcaagt gctgctaaca aaaaagaagc
    92881 tatttgtggt ggtatattag gtggagtagc acttatggct gcagctataa tgatgcactt
    92941 agctattctt tctgatatag gaaacttata ttcaaaagca ataccatcat tattcttagc
    93001 agataagatt tcaccagtaa ttggagtttt attctcaata attcttatat taggtatata
    93061 tacaacagct gtacctttat tatggtcagt aactaataga tttgtagatg ataaacatcc
    93121 taagtttaaa ttaataactt taatagcagc tatattaggt cttataggtg gcttcctacc
    93181 atttgataag ctggttggta ttttatatcc atatactgga tatatgggcg tcataatact
    93241 ggtttgtgtt ctttatagac agattacaaa aacatctgga tatcaagaag gaatcagtga
    93301 caaataagta ttttttaata ttgtaaactc aagagctata tgaaaacaat gaaaatgttt
    93361 tcatatagct ctttttattt tttagctcgt ttattatgaa atagaaaaaa catgtttttt
    93421 agttacatta catatattta taattatgta taaatatata ctaatcaata atttagagtc
    93481 taagataagt ttataaatca tatatttaga atatatggga ggagtgataa gattgaatgt
    93541 aaagtttaat attaaaggta taatttatgg agttatagct cttgtattcg ttataggggg
    93601 atttatattc ataccaagta tgtttgtaga gaaatctaaa cccatagatt atacagtatt
    93661 acaaagaaat tcaataccag aaaagatatt agatgtgatg gataagtata caaatgaaga
    93721 aagagcatta gcggctaaaa tcgataacaa aatatacatt atagtgacta gaggaaataa
    93781 taaatatggt atagaaatgg acaaaataga aagtgtggtt gaggaaggaa aggatgtttt
    93841 gagagtcaag ataaagtata aagataagga agattctttt ccatacatag ttgttgaaac
    93901 aaatatgagt gaacttccag atagaataga attaaatgcg acgaaataag aaggtatttt
    93961 ataattaaat aactgcatat tgtacaataa agtcatggtt tttattaatg taagtaaatt
    94021 gtatatggtt cttattgttg taaaagccat gacttaaaat gcttataaag taagatatta
    94081 acagtagtat ttattactat attaaatggg gtctggaaat acttaatatt ctaattattt
    94141 ggagtgataa ttctagtttc agtaactatg tagtctaatt gtgcatcatg agtttctttt
    94201 ggaatagaat cgaatatctg gaagtcaaat gctatgccga tagttatagc atcttttctt
    94261 aagttttcta aaaatctatc ataaaatcct ccaccatatc ctaatctata gcaatgtata
    94321 tcatatgcta cagctggtac aattacaaca tctaaggcct taatatcaat tgtaggtgat
    94381 ttttcgttag gttctcttat tccatatgct ccaattctca atccctcttt taaatctgtt
    94441 atttgagtag ggattaatgt atgattttct tttattgtga tgggacttga tactattttc
    94501 tttagagaca aaagtttttt gattaaatta tctgtttgaa cttcattgtt aaaatttaga
    94561 taaagcatta tattagttgc atttcttata tcttccattt gaagtaattt ttcagtgatt
    94621 aaatttgaat tactaagtac gaagtttgca ctcttatttt ttcttgattc aataactttt
    94681 tttctaaatt cttttttcat aaaataattc ctcccttaat atgttataat gtgtacagca
    94741 attgtattta atataaattt tagtataatt tattgtaata aagcaatata tttgggtaaa
    94801 tgtaataaaa ataacagcta gtcatataat aataatagtt tatgtaaaag gagatgtatc
    94861 ttgtgatttc aaagaagaaa gctatttttt taggggtgat tttagtaatt ataacagcta
    94921 tggttacatc agcatttcag ttgacattgg ggaataaagt tgtaatatcg aaagaactct
    94981 atgaagacta taaaaaatat gataagttgt taggactaga atctattata aaacaagatt
    95041 tttataaaaa agtatcagat actgatttgg taaatggagc agcaaaaggt ctatttctag
    95101 gaacaaatga taagtattct ggttattata ctaaagatga aatggagaat ctaattaatg
    95161 atagtgaagg ttcttatgtt ggtgtaggta tgtatatagg ggcatcaaaa gatggtggct
    95221 tagtagtagt tcccatgaaa gattctccag cagagaaagc aggtgtaaaa tcaggggata
    95281 aattagtaaa agtcaatgga aaatctgtct catataaaaa ttcagatgaa gctgttcgta
    95341 tgatgaaggg taaaaaaggt aaaacagttg aactgactat attaagagaa gataaacaat
    95401 tgaactttaa agtaaaaaca gaccagataa ttgagaagag tatagaaagt aaagtaattg
    95461 ataatgattt aggatatata gaaattaccc aatttatatc aagtacatat actgactttg
    95521 ataaagcttt aaaagaatta aaagcaaaaa atatcaaggg attagtaata gaccttagaa
    95581 ataatcctgg tggaatgcta gatatatgta aagaagttgc tgatgagtta attggtgaag
    95641 gaactatagt atatacaaaa gataataagg gaaatactga gtacttgaaa tctgataaag
    95701 aaaaattggg acttccaata gtagtgttaa ctaatggaga aagtgcttct gctgctgaaa
    95761 tattaacagc tgcaatcgtt gataataaag aaggtatttc tgtagggact acaacttttg
    95821 gtaaagggtt ggtacaatca gttgtaagat tgaaggatgg aacaggatac aagttaacta
    95881 ctgctcaata ttttacacct aatggagatt atatcaatga aaaaggtata aagcctacta
    95941 ttgaagaaaa agatgaaaat aaacagttag atgtagcaac taagtggctt agagaacaga
    96001 ttgataaata ggatttaatt tttataaact tatactataa aataaagaac attataaaag
    96061 agatgtctca aaatgaactt tttagttcat gagggagtct ggaaatattt atgtgtattc
    96121 tttaaataaa aatttatttt ttaagtgata attaaatttt tacagacagg gctatggttt
    96181 taaaaatcat agccttattt tgatattttt taaaaacaca ttgcaaccca gttgttccaa
    96241 atattatatc atttactttt aaaatatcgt aaactgcata ctatatattc attataatat
    96301 aatttatcta actagaacct cagtttacta attaaattag atgcatagaa taactttttt
    96361 aaacattgaa aataaattaa actctaaaca catttaaata taaatttata tatcattttg
    96421 acttggttaa acaatttcat acttatcgat ataaaaatag gttataatat aaatgttcat
    96481 tatcttataa ccta
//
LOCUS       DAMJYX010000003        96263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJYX010000003 DAMJYX010000000
VERSION     DAMJYX010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 96263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.003"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(173..1774)
                     /locus_tag="PC479_000190"
     CDS             complement(173..1774)
                     /locus_tag="PC479_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2802780.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1885..2562)
                     /locus_tag="PC479_000191"
     CDS             complement(1885..2562)
                     /locus_tag="PC479_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802781.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2575..3270)
                     /locus_tag="PC479_000192"
     CDS             complement(2575..3270)
                     /locus_tag="PC479_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2802782.1"
                     /translation="MDYLKLANHPLLWIVSTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3322..4521)
                     /locus_tag="PC479_000193"
     CDS             complement(3322..4521)
                     /locus_tag="PC479_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2802783.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4782..6827)
                     /locus_tag="PC479_000194"
     CDS             complement(4782..6827)
                     /locus_tag="PC479_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2802784.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7238..8047)
                     /locus_tag="PC479_000195"
     CDS             complement(7238..8047)
                     /locus_tag="PC479_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2802785.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     QKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8131..8664)
                     /locus_tag="PC479_000196"
     CDS             complement(8131..8664)
                     /locus_tag="PC479_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2802786.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8814..10163)
                     /locus_tag="PC479_000197"
     CDS             complement(8814..10163)
                     /locus_tag="PC479_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2802787.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10175..11557)
                     /locus_tag="PC479_000198"
     CDS             complement(10175..11557)
                     /locus_tag="PC479_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2802788.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11801..13015
                     /locus_tag="PC479_000199"
     CDS             11801..13015
                     /locus_tag="PC479_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2802789.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13192..15105
                     /locus_tag="PC479_000200"
     CDS             13192..15105
                     /locus_tag="PC479_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2802790.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15376..16350
                     /locus_tag="PC479_000201"
     CDS             15376..16350
                     /locus_tag="PC479_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2802791.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16371..16646
                     /locus_tag="PC479_000202"
     CDS             16371..16646
                     /locus_tag="PC479_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2802792.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16659..17822
                     /locus_tag="PC479_000203"
     CDS             16659..17822
                     /locus_tag="PC479_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2802793.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            18159..20438
                     /locus_tag="PC479_000204"
     CDS             18159..20438
                     /locus_tag="PC479_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2802794.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20533..21873)
                     /locus_tag="PC479_000205"
     CDS             complement(20533..21873)
                     /locus_tag="PC479_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2802795.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21885..22667)
                     /locus_tag="PC479_000206"
     CDS             complement(21885..22667)
                     /locus_tag="PC479_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2802796.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22682..23704)
                     /locus_tag="PC479_000207"
     CDS             complement(22682..23704)
                     /locus_tag="PC479_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2802797.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23710..24723)
                     /locus_tag="PC479_000208"
     CDS             complement(23710..24723)
                     /locus_tag="PC479_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2802798.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24851..25789)
                     /locus_tag="PC479_000209"
     CDS             complement(24851..25789)
                     /locus_tag="PC479_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2802799.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26059..26523
                     /locus_tag="PC479_000210"
     CDS             26059..26523
                     /locus_tag="PC479_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802800.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26635..26952)
                     /locus_tag="PC479_000211"
     CDS             complement(26635..26952)
                     /locus_tag="PC479_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2802801.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26965..27696)
                     /gene="chbG"
                     /locus_tag="PC479_000212"
     CDS             complement(26965..27696)
                     /gene="chbG"
                     /locus_tag="PC479_000212"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2802802.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27722..29032)
                     /locus_tag="PC479_000213"
     CDS             complement(27722..29032)
                     /locus_tag="PC479_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2802803.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29129..30442)
                     /locus_tag="PC479_000214"
     CDS             complement(29129..30442)
                     /locus_tag="PC479_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2802804.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30464..30775)
                     /locus_tag="PC479_000215"
     CDS             complement(30464..30775)
                     /locus_tag="PC479_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2802805.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30975..31679)
                     /locus_tag="PC479_000216"
     CDS             complement(30975..31679)
                     /locus_tag="PC479_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2802806.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31770..33131)
                     /locus_tag="PC479_000217"
     CDS             complement(31770..33131)
                     /locus_tag="PC479_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2802807.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33630..34784
                     /locus_tag="PC479_000218"
     CDS             33630..34784
                     /locus_tag="PC479_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2802808.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34866..36584)
                     /locus_tag="PC479_000219"
     CDS             complement(34866..36584)
                     /locus_tag="PC479_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2802809.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36589..37578)
                     /locus_tag="PC479_000220"
     CDS             complement(36589..37578)
                     /locus_tag="PC479_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2802810.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37674..38723)
                     /locus_tag="PC479_000221"
     CDS             complement(37674..38723)
                     /locus_tag="PC479_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2802811.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(39000..39668)
                     /locus_tag="PC479_000222"
     CDS             complement(39000..39668)
                     /locus_tag="PC479_000222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2802812.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39674..41047)
                     /locus_tag="PC479_000223"
     CDS             complement(39674..41047)
                     /locus_tag="PC479_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2802813.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(41044..42822)
                     /locus_tag="PC479_000224"
     CDS             complement(41044..42822)
                     /locus_tag="PC479_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2802814.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42939..43262)
                     /locus_tag="PC479_000225"
     CDS             complement(42939..43262)
                     /locus_tag="PC479_000225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2802815.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43249..44226)
                     /locus_tag="PC479_000226"
     CDS             complement(43249..44226)
                     /locus_tag="PC479_000226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2802816.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44241..44804)
                     /locus_tag="PC479_000227"
     CDS             complement(44241..44804)
                     /locus_tag="PC479_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2802817.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44856..45347)
                     /locus_tag="PC479_000228"
     CDS             complement(44856..45347)
                     /locus_tag="PC479_000228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2802818.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45350..47275)
                     /locus_tag="PC479_000229"
     CDS             complement(45350..47275)
                     /locus_tag="PC479_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2802819.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47277..47588)
                     /locus_tag="PC479_000230"
     CDS             complement(47277..47588)
                     /locus_tag="PC479_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802820.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48218..48493)
                     /locus_tag="PC479_000231"
     CDS             complement(48218..48493)
                     /locus_tag="PC479_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2802821.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48608..50017)
                     /locus_tag="PC479_000232"
     CDS             complement(48608..50017)
                     /locus_tag="PC479_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2802822.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50388..51050)
                     /locus_tag="PC479_000233"
     CDS             complement(50388..51050)
                     /locus_tag="PC479_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2802823.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51304..53523)
                     /locus_tag="PC479_000234"
     CDS             complement(51304..53523)
                     /locus_tag="PC479_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2802824.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53799..56453)
                     /gene="adhE"
                     /locus_tag="PC479_000235"
                     /gene_synonym="adhC"
     CDS             complement(53799..56453)
                     /gene="adhE"
                     /locus_tag="PC479_000235"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2802825.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            56957..57157
                     /locus_tag="PC479_000236"
     CDS             56957..57157
                     /locus_tag="PC479_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802826.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(57364..57954)
                     /locus_tag="PC479_000237"
     CDS             complement(57364..57954)
                     /locus_tag="PC479_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2802827.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(57957..58832)
                     /gene="dpsA"
                     /locus_tag="PC479_000238"
     CDS             complement(57957..58832)
                     /gene="dpsA"
                     /locus_tag="PC479_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2802828.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(58908..60299)
                     /locus_tag="PC479_000239"
     CDS             complement(58908..60299)
                     /locus_tag="PC479_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2802829.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(60303..61439)
                     /locus_tag="PC479_000240"
     CDS             complement(60303..61439)
                     /locus_tag="PC479_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2802830.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(61471..62016)
                     /locus_tag="PC479_000241"
     CDS             complement(61471..62016)
                     /locus_tag="PC479_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2802831.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            62340..63110
                     /locus_tag="PC479_000242"
     CDS             62340..63110
                     /locus_tag="PC479_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802832.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            complement(63564..63992)
                     /locus_tag="PC479_000243"
     CDS             complement(63564..63992)
                     /locus_tag="PC479_000243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2802833.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(64188..64421)
                     /locus_tag="PC479_000244"
     CDS             complement(64188..64421)
                     /locus_tag="PC479_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDF2802834.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            complement(64701..66617)
                     /locus_tag="PC479_000245"
     CDS             complement(64701..66617)
                     /locus_tag="PC479_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2802835.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     NNYLKEIRLNLVNF"
     gene            complement(66617..67363)
                     /locus_tag="PC479_000246"
     CDS             complement(66617..67363)
                     /locus_tag="PC479_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2802836.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(67467..68318)
                     /locus_tag="PC479_000247"
     CDS             complement(67467..68318)
                     /locus_tag="PC479_000247"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2802837.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(68586..69590)
                     /locus_tag="PC479_000248"
     CDS             complement(68586..69590)
                     /locus_tag="PC479_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2802838.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            complement(69600..70292)
                     /locus_tag="PC479_000249"
     CDS             complement(69600..70292)
                     /locus_tag="PC479_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2802839.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            complement(70607..71566)
                     /locus_tag="PC479_000250"
     CDS             complement(70607..71566)
                     /locus_tag="PC479_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2802840.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            complement(71599..72477)
                     /locus_tag="PC479_000251"
     CDS             complement(71599..72477)
                     /locus_tag="PC479_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2802841.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            complement(72470..73252)
                     /locus_tag="PC479_000252"
     CDS             complement(72470..73252)
                     /locus_tag="PC479_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2802842.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            complement(73253..73882)
                     /locus_tag="PC479_000253"
     CDS             complement(73253..73882)
                     /locus_tag="PC479_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802843.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            complement(74277..74903)
                     /locus_tag="PC479_000254"
     CDS             complement(74277..74903)
                     /locus_tag="PC479_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2802844.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(74979..75950)
                     /gene="nrdF"
                     /locus_tag="PC479_000255"
     CDS             complement(74979..75950)
                     /gene="nrdF"
                     /locus_tag="PC479_000255"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2802845.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(75963..78062)
                     /gene="nrdE"
                     /locus_tag="PC479_000256"
     CDS             complement(75963..78062)
                     /gene="nrdE"
                     /locus_tag="PC479_000256"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2802846.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            78540..79265
                     /locus_tag="PC479_000257"
     CDS             78540..79265
                     /locus_tag="PC479_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2802847.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(79393..80589)
                     /locus_tag="PC479_000258"
     CDS             complement(79393..80589)
                     /locus_tag="PC479_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2802848.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(80592..81617)
                     /locus_tag="PC479_000259"
     CDS             complement(80592..81617)
                     /locus_tag="PC479_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2802849.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(81689..82819)
                     /locus_tag="PC479_000260"
     CDS             complement(81689..82819)
                     /locus_tag="PC479_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2802850.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(83047..83228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(83281..84174)
                     /locus_tag="PC479_000261"
     CDS             complement(83281..84174)
                     /locus_tag="PC479_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2802851.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(84254..85156)
                     /locus_tag="PC479_000262"
     CDS             complement(84254..85156)
                     /locus_tag="PC479_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2802852.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(85206..86366)
                     /locus_tag="PC479_000263"
     CDS             complement(85206..86366)
                     /locus_tag="PC479_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2802853.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(86522..86827)
                     /locus_tag="PC479_000264"
     CDS             complement(86522..86827)
                     /locus_tag="PC479_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2802854.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(86925..87899)
                     /locus_tag="PC479_000265"
     CDS             complement(86925..87899)
                     /locus_tag="PC479_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2802855.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            88481..89257
                     /locus_tag="PC479_000266"
     CDS             88481..89257
                     /locus_tag="PC479_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2802856.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            89590..90735
                     /locus_tag="PC479_000267"
     CDS             89590..90735
                     /locus_tag="PC479_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2802857.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(90890..91759)
                     /locus_tag="PC479_000268"
     CDS             complement(90890..91759)
                     /locus_tag="PC479_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2802858.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(91877..92590)
                     /locus_tag="PC479_000269"
     CDS             complement(91877..92590)
                     /locus_tag="PC479_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2802859.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKSKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            complement(92961..94349)
                     /locus_tag="PC479_000270"
     CDS             complement(92961..94349)
                     /locus_tag="PC479_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2802860.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            complement(94470..95747)
                     /locus_tag="PC479_000271"
     CDS             complement(94470..95747)
                     /locus_tag="PC479_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2802861.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            complement(95884..96219)
                     /locus_tag="PC479_000272"
     CDS             complement(95884..96219)
                     /locus_tag="PC479_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2802862.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
BASE COUNT        32073 a        16637 c        11024 g        36529 t
ORIGIN      
        1 attaaaaata ttaatttttt cactataaag tataaatatt atctgattat taaaataaat
       61 attaatacta taaataaaaa cgaagcatga tttttattta aataaatctc aaacttcgtc
      121 tttacttact tagatattta tttactttct aaatcctatt atttttaaaa ccttatcttt
      181 ttaatacttc accacattta ttctcagtaa tctctccatt ttctagcgca atttttccat
      241 ttacaattac atattcaaca ccattggcta gtaattgtgg ctttgaaaaa tcaatatctt
      301 ctgacctagg ataactatag ttatccatgt caataactgc tatatcagct atcaaccctt
      361 ctttaagaat acctctattt tttattttaa attgcctagc tggtaatgaa gtaactcttc
      421 ttacaatctc ttccaatcta acaccagact ttctgtacat attgaagaaa attggaaaag
      481 ctcctcttct ttgtagttca aaccatgaca tatccccacc cacactaaac aaacaatctg
      541 aaccaaccat aacaaatgga ttatcaatta ttttatcatc atgtagttta tctgggaaat
      601 cagctctata cattcctcct aaacaaaaga taagttcttc gtctccatct aaaagtaaat
      661 cgagtaataa cttgtcctca tctattcctt tttgtgtagc tatttcacgt attgatttct
      721 tttccatatt aatatcttta gtgtttagaa gtattatact ccccctatct ccaagtataa
      781 atgcttcttt taatataagt gttcttccct cttcatttct aagagcatcc attaccccat
      841 ctatacctaa ttcaaataga ctagatgata ttgccataat aaattgaatt actctttttt
      901 tcttcataca atgcccagaa cttcttggta ctgtgtcaac cattatttca acaccattat
      961 acctagcctt taatatctca tcaaatgctt ctatttcaac tggacttaag tgagatactt
     1021 gaaccctagc accacttttc tgcccaacct ctatggcttc ttttactgca ttatatcttc
     1081 ctatgtaatc tctaatatga gatgtatata taccatcatg ctctttaatc tgccttgcaa
     1141 tatcaacaag ttcatcaata tcagcatact tacttggtat atatgccaat ccagtagata
     1201 ttccaaaagc accacttttc attccatcat ttattatatc tgtaatttca tttttttcct
     1261 tttctgtagg aggtctatcc ttagaacctc ccataacact ccatcttata gtcccatgcc
     1321 ccaacaagaa tcccatattg acagaaatac cacttttaga tataaggtca caatattctg
     1381 taaaattatt ccaacattta tttttatcta taagatactt tttttcttct tcaagtaata
     1441 gaccattttt atacatatat tcatatacat tttcagaaga atatggtgtt atagaatgtc
     1501 cacaattacc atttatagtt gttgtaactc cttgttttag aaacttttca aatttaccat
     1561 ctagtattgc tactatttct tcatgaacat gagggtcaat aaatccaggt gttataattt
     1621 ttccataaca atcaatctct ttatttgtat tgctatttat cttagaatct atttttgcta
     1681 ttaagttgtc tttaattgct atatctccat aaaaaccagg attacctgta ccatctatta
     1741 taaatccatt ttttaagatt aaatcatata tcaaaatatt ccctccttaa aaaccaattt
     1801 ttaaataatc ttaatgtaat ttttatattt gaaaatcaaa ttattaaatt tcatatttga
     1861 aaattcacta ttatttcatt gtaattagtc actaagctta tctttataca tagctcttgc
     1921 aattaatata gtaccaaata cacatataag aactactaga gactgtggta ttataccttg
     1981 ctttgataat ataatagtca atacagatat tacaattgct actagaccta attttttatc
     2041 ttgtaaaaat acttgtccaa aaactgaccc aaatagagct ggtaaaatta agtttaattt
     2101 ttctactact tcagctggaa ttttagatag tacgcttcct cctaaaatta cccctatagt
     2161 tagtatactt atattcacta aaatagatac agctattcct attgtagata ttatcgagcc
     2221 ttcttctgta cctgattcaa cttctgctgc cttttgagct gctattgaac atggtagcct
     2281 catattagat atattaccag aaagaaaact catataagtt cctgggatac ctaagattgg
     2341 tgaataagaa actggttcta ttatgtaatt tggagcactc atactgacaa ttgccattgt
     2401 acttgctaat aatgctgata ttggaggcat aataccaaat ccaaagctca aaattattcc
     2461 aggaactaat gctgctataa tacccaaaga tagtgttagt cttccatacc ttatcataaa
     2521 aggtaaatat tcttcattgt atatagattt tactttactc ataaaactcc ccccttatat
     2581 tactgaagct attatcattc cgctaaacat tgatattgta agcccccact ctcttaacca
     2641 ttttatattt ttcttctttt caagcataca caatattacc attatagcca ttccacttat
     2701 acatgcaatt gttccattat caaaacgcaa caccctatca gcatttagat atgcaaatga
     2761 acccaacatt gctcctagag atattattgg tataaatgat ttcttgccac tagatataac
     2821 attggttatc ttatctagtt tatgtccaaa tagtaatgtg aatattatcc atcctaaaga
     2881 acctaatatc atagtccaaa ctgcatttgt aaatgcaagt tttgtcattt caggagaacc
     2941 taaagttata cctaatgcat ctgttccaaa tccggctgac attgattcaa aaattacaga
     3001 acctataaat gataatctca tccatgctat aggcccaccc atagttacta taagcgatac
     3061 cattccagca aatatagtca ttgaaggccc aatagctgaa atagcactac tcttaacagc
     3121 tgactttatt tgtttatctg ttattcccat tgttttacca actttgcaag attttatagc
     3181 aaatataagg gattgtacca ctactattga tactgctact gtactcacta tccacaaaag
     3241 tggatgatta gctaatttta aataatccat ttataaaacc tccctataaa tcctcaaatt
     3301 ttattaatat ttctaatata tctatttcca aacctgtttt agtactctaa gtatatttcc
     3361 acctagtacc ttctctattt cctcatcact atatccatgc tttactaaat atctaacaat
     3421 gttttttgaa gtttctgttg ggttctcaag accttttaca tattctactt tgggatgttc
     3481 ttttgttcct gttatttttt ttatatcaaa tttagcagct agtactgagt gtaattttac
     3541 atggtcgcca tatactgtat ctggtccaaa agctacatgg tctattccta ctaaattttt
     3601 aatatactca aaatgttcca taaatgactc tattgaatgt tctttattct tctctgttat
     3661 tgtagtatga ggtgctgctt ctacacctat aacccctcct tttttagcac atgctattaa
     3721 tacttcatct ggagttagtc tctttgtgtt ccataaactt cttgccccaa catggcttaa
     3781 tattattggg tccttgctat attctattac atcaagtgta gtttgaactc caacgtgaga
     3841 gcaatctatt gccattccaa ttttattcat tctttcaaca gctttttttc cgaaatttgt
     3901 aagacctcca tctttctctt cttttagtcc tgaacctaat gcattggatt cgctgtatgt
     3961 tatacccatt aaccttatac caaatccata cagtatatct attctatcaa gttcattttc
     4021 tattggtgct gcaccttcta atgttggtat caatgctact ttaccttcac ttttagctct
     4081 gtatatatct tctacttttt cacactttat aacaaaatct tgatgagcta aatcacatag
     4141 tctcatgcct aaatctatta atatatcatc ccatttccac cctgaatttg atgttatcgt
     4201 acatacacca tccattaaat tatcaaatat agcatcataa tatcctcttg ataatgcttc
     4261 ataagcacat ctttgtttac cttccctatt atattcaaat atatctcttt ctaggtgttc
     4321 tggaaataat actggatgtt catgtaatga tataaatatt ttttcatcca ctatactttt
     4381 aaccttagct tcttgaatat catctaattc aataaggtat tctttaactc ttttatctcc
     4441 tttacacatc tcaatagatg aaatatcttt tcctttttct aaataactat aggcttcata
     4501 tcctttgtaa gctgacttca taacaacatc ctccaagttc tacaattttt tatatttttc
     4561 ctatgtagca attgatgtgc caaaatataa attttaattg tataataaat cttgcaaaaa
     4621 ataaaaacag tagtaaggtt gatatttact actgttttat taaaaataat cttcttagat
     4681 tatttattca atatttttga attatcttaa tcttaaagat aattttattt atttctatcc
     4741 attataggga tttttttagg gtgttttgcc tttttagtcc cctatatcac tcccttaatt
     4801 tttacatatg atataaagtt ctttccttca tcagttataa aactaccttg tttttttact
     4861 ccagataaaa gtaaaccatg gctctttaac ttatctattc tttttctaat ttggtctaca
     4921 cttaaaacta tatctttttc ttttaaaatc tcttgcagtt tatttcttcc taataatata
     4981 tttttattat tccaagtttc tatactggtc aatatacata tactttcctc aaagaaatta
     5041 gattgtttga aatctaagat aattgaatcg aaattttcat taactaaagt attgtttttt
     5101 tcaaatctaa attgttctgg caaatgctcg taatctactc tatcttcctc aactatagta
     5161 tcaatataat aaattaaatt tttaagctct cttacatttc ctggccattc atacaatgtt
     5221 aaagcttgca ttgattcctc tgtaaaacat ttgttgctat ttatttcatc taaaaaatac
     5281 ttacttataa gaggtatatc ttccttacgt tctctaagtc taggtatctc tatatgtaat
     5341 acattaatcc tataatacaa atcttctcta aaagaacctt ctttaatttt tttctttaaa
     5401 tctttatttg ttgcagctat aattctaaca tctattggta taattttact tccgcccatt
     5461 ctcataactt ctttttcctg taatactctt agcaatctct gctgtacatc taaagatata
     5521 tcacctatct catctaaaaa aattgttcct gtatgtgctc tttcaaatat acccattttt
     5581 ccacctttta ttgctcctgt aaaacttcct tcatcatagc caaaaagttc actttcaata
     5641 agtgtatctg acaaagaaga tagattaact gcaacaaatg gcttgttttt tcttagagat
     5701 tcattatgta ttgcctgggc aaatatttct tttcctgttc cattttctcc aagtatcaac
     5761 acagaaaagt ctgtagttgc tatttttctt gcaatattaa ttttttcttt tattatttta
     5821 ctctcaccta caacactctc aaatgtatac tttgatacaa aaccttttga ctgaagttta
     5881 ttttgaatct tgtcctcaag cacttgaatt gcacttatat ttttaatgct tataatactc
     5941 tttattcgtt cattattttc ataaacattt actttattaa ttatcagttt tttgttattt
     6001 aaatttacta cttcatcatt aacttcatct tcttgaaaaa atattttttt gacaacctta
     6061 tcactaaata agtccataaa gttattagat ataatttcat tttggtctat tccaactaga
     6121 ttagaaaata ctttgttgca atatataaat tttcctaatt tatcaacaga agcaatacct
     6181 tcatctataa tctccaaaaa actcttcatg gttttattca tagttgtata atacctatat
     6241 ccacttactg tatcttcatt gtatttttcc ttaataatat gtagtttatc tatagatata
     6301 tttagttttg taaagatttc tataactgta tttatatcaa ttaccttatc acctatgtca
     6361 attatttgct ttatgttttg tggtatgctg tttctgcttc ccgtaataat tccaatctca
     6421 catttagact tatcacaacc tggataatat ggtattagat taatatgaat taatcctagt
     6481 tttctaatta tcttggcagt ttcatctgct gactctttat aagcatctat aaccataaca
     6541 tcagaatcat tttctatact tattatctga tttatatttt caatatttat agtacgatgt
     6601 actattacaa taggtatatt tttatcaata ttattatgta tatattcctt tatatcattg
     6661 tctgaacata ctacaagctc atagcttgac agttcaatat ttatcctatt tacatatacc
     6721 aagttatcta tattacaaaa ttctccaaat atattttcca attgttttga tatatctata
     6781 tttattttgt catcagttga tacaaataaa atattatgtt ttttcatagt cccacctcct
     6841 gtttaacgag tatagaaagt aataatttgg tctattgtag cactttttaa aaaagtttta
     6901 aatcttaaaa atcaaatttt gtaccattta ctattatttt ccataataaa ataatgtaag
     6961 ctatcttttt tcacatataa tttaatttct aaaacggtta gtttgacatt attggaatat
     7021 aaataaaaat ttaatataaa ttcgttaata tattaataca tttttaagaa tgacaaggaa
     7081 taaattataa caaattttag gaatatataa taatagggtc tcagaactta gttacaagtt
     7141 ctaaaaccct atttgtttag tgtaaaaaac atcataaaat ttagcttaaa tcttttcaca
     7201 taatattact ctttttctaa gtgataataa aatatatcta ttcctctaaa aactgtaaaa
     7261 attcctgatt aaatagttta agctcatcat agaatatagc atgaccacta tactcaaaag
     7321 tatataaaat tgaatcttct attttttcat tcataaaaac tgcaaattca tacggacata
     7381 tttcatctaa tttcccatga aatataccag taggcactct tacacatttt aaatcattaa
     7441 ataattcttc atctcttaaa gatacagcag ttccaatagt gccaatacct gatgcactaa
     7501 aaccaatatc attaaaccat cttctaaagc tttctggtgg atttgatgca aatacttttt
     7561 cgccaaaatc ggttaccatt tctggtctat cagtacatgc ttgtgctatc aatttattta
     7621 catcttcacg tgtcatacca tatggaaatt ctggaggtct ttgtacaaat gatggtgcag
     7681 cagctgcagc taataccaat tttgacactc catatccatt aaataaactc atatacctaa
     7741 gtacgattgc tcctcccatt gaaaaaccaa caagggtaaa gtcttttaat ccaatagcat
     7801 gtaccacctt ataaatatcg tctgctaatt ggctataagt atatcctcct gatgttgcat
     7861 cagatttacc aaaacctctt aaatcaattg aaactgttct atatccgagt ttttgcaata
     7921 tatttgtttg atattcaaat atcttatgtc ctaagggcca accatgtata aaaagtacag
     7981 tttttttagc actcggattc aaatcatata ctgctatgtt tatatcattc acatatacat
     8041 aatacatatc aacagcaact cccttcaaga aaatgtatga tggttatttt atatatatga
     8101 ataaaatgat gaataactaa attttcaaat ttatttacaa tacttctcta ttgccttttc
     8161 aaataaactt acatgaagtt tttcatctag gattattcta tttaatatag cttttatata
     8221 agggtcttca ataagactta tatctttata ataattatta atagccacat gctccaaatg
     8281 caaatctagt tttaatctgt tacaaataga atttccataa catacaaaac caccattcca
     8341 atatgcacca caagaacttg tagaacctct atatacagga tttccaccca ataatcttat
     8401 agtctttgct aatatctcca tatgtaacat ttctgttata gatatattta tccacatttc
     8461 acttaactct ctatgcgttt catctaaatc aaaattatgg tatagatatt gatttacact
     8521 cgtaaattca cttgatactc cagcatagtc atccatcaat aattctgcat aatacttatt
     8581 tggacctaaa acttttatct ctggatatgg ctcatcactt gaatatccat ttcgcttgtg
     8641 catgtcataa tgtgcaccac tcataatatc tcctccttta aatttaatgt atgatattaa
     8701 ttgtatgcat cacaactttt tcatataact tagataataa aatctactta aataataaaa
     8761 agccatatta attaaaatta acatgacctt ttatccacaa aattactttt taattattca
     8821 tcttttccag ctactgatag ctctttaata acaactgaag gtgaacctat actactcatt
     8881 ggaaattcta aatcttctcc tatagcttca atatctttca acaggtcaaa atagtttcct
     8941 gctactgtaa tttgctccac tggaaatact ttttttcctt ctttgatata aaagcccttg
     9001 gcagctaaag aaaagtctcc tgttactgaa tttgcacctg aatgaagtcc tgcaaagcta
     9061 gtaaccatca atccttcatc tatatccttt ataatttcat ctaaagattt atctcctttc
     9121 tctatataaa aattagttgg atatatgctt ataggtgatg aatatgaaga ttttattcca
     9181 tttcctgtag tctttacacc tgccttatta gccgttttta agttatgaag taaagttata
     9241 agctttccat ttaaaacaac ttctttttta aatgtagcta ctccttcatc atcaaaagga
     9301 gtagatgcca taccattttc taaaagtggg tcatcaacta ttgtaactat aggagatgct
     9361 atcatgtctc cttctctatt tttcaataaa gataatcctt tttgagctga atctgcatta
     9421 aatatcccag aaaatgtgct aagcaaagat accatagctt cattaagcaa tatagtttta
     9481 tatttacctg atgtaatact cttaccacct actttagata aagcattatc aacaccaagt
     9541 tttgctatct cacaaggttt tatttcttca atagaatttg ctatattata tcctattcca
     9601 tcatattttt gtccattgtc ttctatgata ggaactacaa accctataag catattagtt
     9661 ttattgctta aattaagacc tttagtatta gatataccat tacttgatac agaataagat
     9721 atagtacatt tagataaatt taccacctta tctgaataag ctttagtctc tctttccatt
     9781 tctaaagcta aatcaatcaa cttatctgcc tctaaatttt ctagctcttt agaataagtc
     9841 tttacatcat tatagtgctt atctccttca tatataaatt gtacatcttc attttcaata
     9901 gtgtttactc catcttttac attttttatc aacatatcaa cagctttatc atctaaaatt
     9961 tctgtataag aataacctat ctttccattt accttacctc taaaagataa accaaaagat
    10021 ttatcaagat tgtatttctc aacttctcct tcatatatat ttatacttaa attttctcct
    10081 gtataataat atatttcaca ctcttcaaaa ccttcactta gagccttttt aaataatata
    10141 tctttaaaat ttttaagttc cacaattatc cctcctatct tccacctaca gtaatttctt
    10201 ttacccttat cataggttgg cctacattag ttggtatact tcctgatgat gagccacaca
    10261 tcccctgagc ttgttttacg ttattcccaa ccatatctat attcataagt atatcgctac
    10321 cttttccaat taaactagca cctctaactg gctcttgtat ttctccattt tttattaagt
    10381 aaccttctga aactgcaaaa ttaaactctc ctgttacagg atttacagaa ccaccaccca
    10441 tttttttagc ataaagtcca tctggtattg actttataat atcttctggt ctatcttctc
    10501 cagcagcaat ataagtgtta gtcatccttg aagttggtgc aaatttgtag ttttgtctac
    10561 gagaacttcc tgtaggattc attcccattc gtctaccatt aagtttatct atcatataag
    10621 acttcaaaat tccactttca atcagtatat tcttttgagt tggatttccc tcatcatcta
    10681 tattcataga accccagtag tttggtattg taccatcatc tatagctgta atctttgtgg
    10741 aagcaatctg ttgacctaat ttatctgcaa atacagagtt cccctttgca actgatgtag
    10801 cttccaaaga atgtccacaa gcttcatgaa atattactcc accaaatcca ttatctattg
    10861 ctaccatcat attacctgca ggacaatttt tagcatgcaa catagtatgc gctactctag
    10921 ctgattcttt tgcataatat tctgggtcta tttcttcaaa tatttcaaac cctttacaac
    10981 ctccaggacc ttcaaatcca gtttgatttt cattaccctt agatgcaatt gaacttatcc
    11041 ctagtctagt tcttattctt ctatcctcaa catatatacc ttcagtgttt gcaattaata
    11101 ttttttggtc tttatccaaa taactgacac ttacttgaga tatatcatta ctaaattctt
    11161 ttgcactctt atatgcaacc ttcataacac ttactttatc tttatttcct attgaatttg
    11221 gataaatttt tatattatgt atgttattta ttttaatact gtcatttaat attacacttt
    11281 ttccatcttc caatttacct aaagcaacag cagctttgta tgctacatcc aacagactat
    11341 ttaatgaatt gtcacttgta taagcatata tacttttcaa tcctttaaat attcttattc
    11401 ctataccata atttcttcca cctattgcat tttctacttt attatctatt aaactaatag
    11461 aattatttat agtgtcttct tcaaaaatct cagcaaaatc tcctccagta attagacatt
    11521 tttctaatac cttagaagct atattttttg aaagcataaa tttacctcct actttaaatt
    11581 tttaattaac ttttattcaa tctttttact tgttaacaaa cattatatac tatattactt
    11641 aaaataaggt aattatgaat atattataca tatgttttat actaaaatgt tatcacattt
    11701 tatctataca tacccatata actagataat taaaatgtat aatattattt gctatagaaa
    11761 ataatacact catacataat aaattttagg agtgaagtat atgaatcaag aatatgtaaa
    11821 aggaaatcct atttttacta atattaataa agttccaaga caatttccat accttactga
    11881 tgatattgat actgatgtta taattgttgg tggtggcgta actggatgta tttgtgctta
    11941 ctatctagct aaaaacaata taaagtctgt cattcttgaa aaaggaagga ttgcacatgg
    12001 aagcactagc gtgacaactt ctctattaca gtatgaatta gatgacaatt tgatagattt
    12061 aacagaggtt atgactctaa aagatgctct aaaagcatac aacctttgta cttcagcact
    12121 tgaagaatta gatacattta tagagctata tggaaataag tgtgattatg ccaaaagaga
    12181 tactctactt tatactgcca acaaacttga agttaaggct atcaaagaag aatataactt
    12241 aagaaaagaa aatggatttg atgttgaata tattgatgaa tccacaaacc cttttagctt
    12301 tgatttaaaa tcaggattga tagcaaagaa tggaggacga gaattagacc catataaata
    12361 tagtcaccat ttgattgatg taagtttaaa aaatggtctt caagtatatg aaaatacaga
    12421 agtaaaaaaa gtagatttat caaatgataa ggttactgct gaagtatcgt atggatacaa
    12481 agttcatggt aaaaaattaa ttgtagctac agggtacaat acaagtttat ttacaaaaag
    12541 aaattttgca actaagtcaa atacttttaa tatagcaacg aaaccactta aaaacatagc
    12601 ttcttggaaa aacaacattt taattagaga taactgtgac ccatataatt atctaagaac
    12661 tactaaagac aatcgattaa ttataggtgg tgaagatgta agttttgatg atatagaaaa
    12721 tgaaacttta gccaatgaaa agtatgatat tttagaacaa agattgaaaa gtatgtttaa
    12781 ggatataaaa gacattgaag ttgaatataa atattgtggt tgttttgctt caactttaga
    12841 taatttaggt tttattggtc cagataataa aaataacaat ctatggtatt gcttaggtta
    12901 tggagcaaat ggtatactct ttgcaatatt aggaggtata atgttaagtg aattatatct
    12961 tggcaaacaa aataaaaata tgaaattatt taaagtagat agatttgata aatagatatt
    13021 atatattaaa agactgtaaa aagatgaaac tttataattc taaaagttaa ctttgataca
    13081 gtcttttata atgttccttt taaaaatact ttatttataa ttaaatataa agtaaaatgt
    13141 taaaatataa ctaataaaat ttagtcataa gataatattt aagtttattt gatgaaatat
    13201 ggaggaatta ttatgaagga aatactcgtt attgctccca ctaaaggaac ctatgaaaaa
    13261 tcaattcaca tagttaaaaa aaacaaatat acaaatattg atgttgtatt tggcaatcta
    13321 aaagaaggta tacctcttgc tgaaaaaagt attaatcatg gtactagaat tatcataagc
    13381 cgaggtggaa cttacaatat gttaaaagct acttacaata tacctatagt tgaaattaaa
    13441 gtagatgctt atgatattat aaaaagctac aaggaggtaa aaaattccaa cgaaccattt
    13501 ggaataattg gatttaataa tgtcatctac ggtttcgata ttatagaaga aatattgaat
    13561 aagaaaataa ctatgattga aattgaaaaa gaagaagaaa tttatgatgc tatagaaaag
    13621 tacagaaaga aaggtataaa cacatatata ggagatacca ctgttgcaca tattgttaag
    13681 agacttaatt gtaagggaat attaatcgaa tctagagaag aaaacatact tagagccatt
    13741 caacaagcag aacaaatact cgaagcaacc aaggatgaac aaaaaagaag attacagata
    13801 gaagctatga cagattttgt acatgatgga ataataacag ttgataaaaa ttttaaaata
    13861 acattattta ataagagtgc tgaaaaaata tttggtataa aaaaagaaaa tgcactttat
    13921 cataatgttg tagatgttat cccaaacaca gttcttccac aagtagttga gactatggaa
    13981 cctcaaattg gtgaaataca aaatataggg agtacaaaaa ttattacaaa tagagttcca
    14041 ataattgttg atggtcagat tcaaggggct gtttcaacat ttcaagatag caaggagata
    14101 agttcttttg aacatacaat aagaaggagc ttattaagca aaggtctatc tgctaaatac
    14161 acttttgatg atataattca tgctagtgaa aagacaaaag attgtataga acttgcaaaa
    14221 aaatatgctt gttatgatac tcctatgatt attactggag aatctggagt tggtaaagaa
    14281 ctattttgtc aaagtataca taactatagt aagcgtaaaa atgcaccttt tgttgctgta
    14341 aattgtgctg ccatacctcc atctctaata gagagtgagt tttttggtta tgaggaaggt
    14401 tcatttacag gtgctagaaa aaaaggtaaa gcaggtgtat tcgaactagc acatgaaggc
    14461 acaatcttcc tagatgaaat aagtgaaatc cctcttgaac ttcaaggtcg attattgagg
    14521 gtattgcagg aaaaacaagt tatgagaatt ggtggtgata aagttattcc tatagatgta
    14581 aaaattatct gtgcttcaaa taaagactta aaaaatatga tgcgcaatgg tttatttaga
    14641 caagaccttt attttagaat aaatatacta acattatatc ttccttcatt aaaagaaaga
    14701 aaagaagata ttataaaatt atcagaattt tttatacata aatattcaag caaatacaat
    14761 aaaacgcctt taattatcac tccaaatatt aaaaaggcac ttcttgaacg agaatatgaa
    14821 ggtaatataa gggaattgga aggtatgatg gaaagggctg ttattgtgga atcatttgac
    14881 tcgattttat tagaaaagct tagcaatgaa tatacaaata atatagttga taaagataat
    14941 agtgattttg aatgcagtaa tttagattta aaaactttag aaaaaaaata tatctataaa
    15001 atttataacg aaaataatag aaatactaca aaaacctgtg aaattctaaa gataactcgc
    15061 tccactctat ggcgtaagtt aaaagaaata gaagatgatg tttaaaaata atacatatct
    15121 atatatatca aaacgtttta tgtttaattt taatacataa gacgtttatt tgttttcaat
    15181 aaaaaataca attatatgct aaaaagaatc tttgtaagtg aaattctcgt caatcatact
    15241 tttaattata agtaatctac agcatttcaa tcgattatta aaaataatca tgataactaa
    15301 agatacaaac tctatatatt ggcatagtaa ttgcttttat aatacctata taaaaatgaa
    15361 agggagtgat actagatgca catcttaaaa tcagtgaaaa agataccagg aggacttatg
    15421 gtctgtccct tgttattggg agcactattt aatacagtat gtccacaagc tcttgaaatt
    15481 ggaggattca caacatcctt atttaaaact ggtgcaatga gtatacttgc tctattttgt
    15541 ctatgcaatg gagctcaaat caatatcaga caagctggta aaccattgac taaaggtata
    15601 gtacttacag caacaaaatt tataattggt gcagtattag gtattattgt tagtaaattt
    15661 attggaccta aaggtataat tggtattaca cctcttgcca tagttgctac catgactaat
    15721 tcaaatggag gtctatttgc tgctcttgca ggtgaatatg gagactccac agatgttgga
    15781 gcaatatcta tcttatctct aaatgatggt ccttttttta caatgcttgc ttttggagta
    15841 actggacttg caaatgtacc tatagtagca ctatttgcag cacttatacc aatactactt
    15901 ggatttatac ttggtaacct tgatgaagat atacgagaat ttcttgcacc tggtacaaca
    15961 ttattaattc ctttcttcgc ttttccttta ggagcagcat taaactttaa tcaaattata
    16021 actgctggac tacctggagt tgtacttggt ttaggagtta ctataataac aggtatgggt
    16081 gggtactttg taatgaaact tatacgtgca gaacatccag cagtaggtgc agctacaggt
    16141 acaacagccg gaaatgcagc tggtactcct gaagctttag cagctataga ccctactcta
    16201 gctacaatag cagctgtttc aacagtacaa gttgctgctg ctattattgt tacagcaata
    16261 tgttgtccaa tgttagtttc ctatctagat aaaagagaaa aaaagaaaaa atctattttt
    16321 aaaacaaata aaaatattga agtaaattaa tacttaagga ggtaaaatta atgaatgcaa
    16381 ttattcatgc aaatgaaaaa gataatctag ttacatgtgt cagaccttta attaaaggtg
    16441 aaaaagtttc aattgatgga aagtcttaca tagtaaatga taatatacct gtctttcata
    16501 agatggctac agaagatgta aaaaaaggtg atgttgtata caaatacggt gaaattatag
    16561 gaattgcaac agttgacata aaaactggtg actatgttca tgtacataac attgaaagta
    16621 cacgtggtcg tggagataaa aaataggagg aagttaaaat gaaattaatg ggttatttaa
    16681 gagaaaatgg tcagtttggt attcgtaatc atgttttaat tattccaaca tctgtatgtt
    16741 caagtgagac agctacaaga atagcatcac ttgtgcctgg agcaatagca atccctcatc
    16801 aacatggatg ttgtcaaatt ggttctgata tagagcttac agcaaagaca ttaataggat
    16861 ttggaaaaaa tcctaatgtt gccgcagttt tagtagttgg tcttggatgt gatggtattc
    16921 aagcaaaaga attagcatct gaaatagcta caacaggaaa aaaggtaaat tacgttgtta
    16981 tacaagaatg tggtggaaca ttaaaaacag tttctaaggg agctgaaata gtaagtaaaa
    17041 tggctcgtga agtatcaaaa gaagtaagag ttgaatttgg tatgagtgaa attacattag
    17101 cacttgaatg tggtggttct gacccaacaa gtggaatagc ttcaaaccct tcaataggcg
    17161 ttgcatcaaa ccttcttgta gatgaaggtg gaagtagcat actaagtgaa actacagaag
    17221 taattggtgc agaacatttg ttggctaccc gttttgaaga tgaagaaatg aaagataagt
    17281 ttcttaaatt tgtaagtgat gtagaaaaaa gagcaattgc tatgggagaa gatttacgta
    17341 gtggtcaacc tactccaggc aataaagctg gtggactttc aacaattgaa gaaaagtcac
    17401 ttgggtgtat gtataaggca ggcaataagc cattcaaagg tgctcttgaa tatgctgata
    17461 ttgtacctcc tgataaaaaa ggattatatt ttatggatac tccaggtcaa gatatagatt
    17521 ctatcactgg tatggtagca ggtggtgcac aaattgtaat cttttcaact ggtagaggta
    17581 ctccaacagg aagccctatt tcaccagtta ttaaaatcac tgggaatagt gatacataca
    17641 acaaaatgcc agacaatatc gatataaatg caggtagaat tataacagat ggtgctaaaa
    17701 tagcagatat aggtcaagaa atatttaatg aaataataga agtctgtaat ggtaaacata
    17761 ccaaagctga aagtcttggt catagagaat ttggcattta cagaatttca tctacatttt
    17821 agattgtaac aaaatatctt ttataaatat aatacttatg tagatacata agctaaaagt
    17881 tctttctaat actataaaaa ttttctatac tgaaaaggaa ctgtatcaaa attattttga
    17941 aaacagttcc ttttttaacc tcttttactc aaaatattat aaaataagat ttaaacaata
    18001 aacgtatctt gattaatctc taagaagaca taaataaagc taaatgtaaa gtgggataaa
    18061 taaaatttaa tatctttgta tgaaaagata tttggtataa tatgtctgag agaattctta
    18121 tttgtaaaca aataaaggat ggaggatgaa acaaaagaat gaactttgaa aacacaaaaa
    18181 ataaaaaaat aatagctact ttactattga cactcctttt aatcgtgtca ttaacatgtg
    18241 tcacttttat tatgaatcta aataaagtaa cacataatga aacagaacga tatcttgtag
    18301 aagtttcaca aagtatttcc actacaatta atgtgaaatg taaaagtata tttaggacac
    18361 tatattctat gggagatact tattttgaga ttaatgatag tgaaaaagaa gtcaatagat
    18421 acttatcaaa aaaattaaaa ttatttgaat ttgattggtt aggtattata gattctaatg
    18481 gaaattttgt aagtgccctt aaagatacag gtaatgttaa aggacaagac ttttatagaa
    18541 aagcccttca aggcaaagaa acaattactc ctgttaaatc acaatctggt acatatggaa
    18601 ttttatattc tgtaccacta tacaatggtg ataaaattgt tggagctgtt gctgcttgga
    18661 atactattga tactatgaga aatttattaa gtgtagaaag ctttcatggt gaaggatttt
    18721 cccaaattat cgaccaagat ggaaatttta ttgtaagttc agcaaacaaa aatgctccaa
    18781 aaaatgtggt aaattttttt gacatgatta aacaccaagg aaaacaggtt gataaaaata
    18841 aacttgaaga aatgaaaata aacttaaaaa agaacaaaac tggttctatg tattatacat
    18901 taaatgatgg tatttctaag gcaatgcatt atcttccatt acaagaagaa aacttatacc
    18961 tcctttcaat tgttccaaca aaaacagcaa gtgcacagtt tgatttactt ctaaaaaaag
    19021 caatcttcat caatatttta attgttatac tatttttatt gcttattata ttgattatgt
    19081 ttataaatca aaagaacagg gcacaactta taaaaatcgc ctatgtagac tctgtaacta
    19141 aaggttacag taaagcacgt tttgatattg aagctaaaaa aattatacag tcatcttcat
    19201 cagaaagcta ttcattggta tctataaata ttcagaaatt caaacttatt aatgattctt
    19261 ttggtagtga agcaggagat aaaacattaa agtacataca tgatgtcatt agtaattatc
    19321 tagaaccagg agaattgctt tgtcgagttt ctaatgacca gtttaatgta cttattaaaa
    19381 ctattcctca aaaagaaatt cttaagtaca tagaaaatat caccaatgat attaatagtt
    19441 tcaataaaaa tgaagaatgt aaatatttta tagctctatc tataggtgta tataaaattg
    19501 atgacccaaa tctaccttta gtttcaattc aagaccgtgc aaatgttgca cgaaaaaata
    19561 ataaaggaaa cattggaaat catccacttt atacatgtgt tttttatagt gatttagagc
    19621 gtttaaaaat gattaaagaa aaagaaatgg aaaatagaat ggaggaagca ttaaataata
    19681 acgaatttat agtatatctt cagcctaaaa taagcctcaa agataatact attgtaggtg
    19741 cagaagcatt agtaagatgg caagacccaa ataaaggtct tataccacca gacgagttta
    19801 ttcctttctt tgaaaaaaat ggttttatta caaaactcga ccactatgtt tttgaaaaag
    19861 tatgcagtat gataagaaaa tggatagata atggagaaac tccaatacct gtttcagtta
    19921 atttttcacg tgctaattta aataatatag attttttaga aaaatacaga atgcttcgta
    19981 ataaatacaa cattcctgct aagctcttag aaattgaatt aactgaaaca cttgtatttg
    20041 aaaatctcca aaaattaatt aatataatag accaaatcca tgaagaaggt tttcaatgtt
    20101 cactagatga ctttggaagt ggatactctt cactcaatat gttaaaagaa ataaaagttg
    20161 acacattaaa acttgaccgt gcttttttta gctcacctaa tgcagataat attgctgaaa
    20221 atcatgtaat agaatctata gttaatttgg caaaaaaatt aaatatgaat agtatatcag
    20281 aaggtgttga aacaatcttg caaatggatt atcttaaaaa aattaattgt gatatgatac
    20341 aaggatatgt tttttcaaaa cctctacctc aagaaatatt tgagaaaatg acttttggaa
    20401 aaaaactaga agaaaccagt atggatagtt ccgtttaaat aaaataaaaa acttgtctct
    20461 ataaaataat tttaatcatt ttataaagac aagtcttttt attttccaat ttaattttat
    20521 tttctaaatt tcttacactc ggcttgtttt aaattccctt tttaagaata ataatacaat
    20581 tatcatagca ataaaatctg caactggctg tgccaaccaa acaccatcta aattaaattg
    20641 aattggaagt acaatgatta atggtataaa taaaataaat tgtcttaaaa gagttaaaaa
    20701 cattgaatgt ttaactttac ttatacactg aaaatacaca ggtcctacaa tagaaatccc
    20761 cattataggc aatgtaatta gatttaggct tatacctctt actgctatat ccatcaaatc
    20821 tgaatcttta ttaaatatac ctacaaatac ttctgggaaa acttgtacta ctaaaaatcc
    20881 aaatgacaat atgacaactg acactatgat agataatatt aaagttttct ttgcacgctc
    20941 atattgtttt gcaccataat tgtatgatat tatagtttgc ataccttgat tcaatccaaa
    21001 cacaggcatc ataaacatca ataaaataga tgtaagtgct gtcatagctc caatagctaa
    21061 atctccacca taaaccttta gagaattatt tgtaaacatg tgagttatac ttatagctac
    21121 ttccatagca aaaggtgtca ttcctattgc aaataccttc tttactatat ctttattcaa
    21181 ttttaagtta cattttttta gttttaaatt ggacttccct cttataaaat aatgtattac
    21241 ccaaaagaat actactattt gacagagtac tgttgctata gctgctcctt ttatacctaa
    21301 attaaataca aatataaata tagggtccaa tatcaggttt aatacacatc ctacaatcat
    21361 tgtccttgct gctaattttg gattaccttc tgccctaatt gcattattaa gagaaaatgc
    21421 aactaaatta aatatacttc caaccaatat gacactcata tagtcttttg cataaaatat
    21481 tgaatcttta cttgcaccca aaataaaaag tatatcttct aagaaaacta atcctaatat
    21541 agttataatt aaagagataa ttatagatag tgtcaaagca ttacctaata tcttctctgc
    21601 ttcttctcta tttctttccc ctaactttat agacaatctt gtgctagcac ctactgatac
    21661 caacattcca aaagctatta ttagagtaaa tactggcata gttacaccaa gacctgcaat
    21721 agctatagag ccaatacctt ccatagaacc aataaaagct ctatctacta cattataaag
    21781 tgaagttacc atcattgcta ttattgcagg tatcgaatac ctaaataata aaatccatat
    21841 tttttcatgt cttaatgctt cttgattctc catgttgtca ccatttacac catgtcataa
    21901 gttatacaaa ttggtttatt tgttcttggg tctgttgcta tttctgcatc aatattaaat
    21961 aattctttta gattatcctt cgtcataaca tcatatgcat caccttcaca aactatttta
    22021 cctttcttaa tacctatcat gtggtcagca aatcttgctg cattattaag ttcatgtatt
    22081 accatgacaa tagttcttcc ttgtttttta tttagttcat ctaataattt tagtatctcc
    22141 aattgatgtg ccaaatctaa atatgttgta ggctcatcca gtaataatat atcagtttct
    22201 tgtgccaaag ccatagctat ccatgccctt tgtctttgtc ctcctgataa atcttccata
    22261 tttctatcct taaattctgc aattccagta acttctaaac tccaatttac aatctctctg
    22321 tcttctttac tagaattacc aaatccactt ttatgaggaa atcttccata agaaattaat
    22381 tctgaaacgg taagaccact aggagcttga gggctttgtg gaagtacagc catatctttt
    22441 gctatttctt tagacttctt tttgtagatg tcctctccat ttatatatat tttcccacta
    22501 ttagggctaa taattctacc tatagtttta agtattgtcg attttccaca accattagcc
    22561 cctatgatgg tagttatttt tccttttggt atatttaaat ttaaattttt tataatatct
    22621 gtgtttccat atgatacatt tagattctta gtatttatac agttcataac aacacccttt
    22681 cctaatcatt taccaacatt aaataaataa agtacggcac tcctattata gaaactacta
    22741 tacctactgg tatttctata ggtgctagaa gatttcttga tatagtatct gctattaaaa
    22801 gtaataatgt tcctatcaat gctgatactg gtattaatct tttatgcttt ggtcctatta
    22861 tttttctacc tatatgtgga gctattaaac ctaaaaatga aatactacct gctactgctg
    22921 tcgatacacc agataaaatt actgcatata ttataagtct tcttctttgt ttttctacat
    22981 ctacaccaag acttatagat acttcatcac ccaaatttaa aacatctata tatttagatt
    23041 tataaatagt tatacccata aaaatcacta taaatggaag cacagctatt acatatttcc
    23101 aactggttcc ccaaatgctt cctgatgtcc atgccataac cctattaaat tcctgtgttg
    23161 taaatttcaa ttggaatatt gtcaaaattg aagtaaaagc tatgtttaca cctataccaa
    23221 taagtagtaa tcttgatgaa tttataccac ctttccatgc aagagaatat ataagaaatg
    23281 ctccaaataa agctcctata agtgcaacaa taggcattgt aaatacagtc aagttactca
    23341 taccttcata tacattacca ttcataaggt atatataaac tactacaaac aatgcagcac
    23401 ctgaatttat tcccaaaatt ccagaatcag ctaaatcatt ctttgtaact ccttgaagta
    23461 ttacacctgc tgttgataat gcagaaccaa ccaaaagtgc tattactatt cttggtaatc
    23521 ttaatttaaa tattgcaatt tcatgattac gctgaccctg ccctaaaaaa gtctttataa
    23581 catccattgg ttctatagaa aatgagccca tatttaaact caataaaaaa gtgataaaaa
    23641 taagcaccat taaaattgat ataacaagta atgttttaga atttattttt ataatatttt
    23701 tcatagtacc tatttctccc cctttctaac aaggtatata aatattggca ctcctataag
    23761 tgctgttata gaacctactg gtgtctcata tggaggattt atcatcctac ttaatatatc
    23821 tgtatataca agcaatacag ctcctaatac taaagagctc ggtattatat acttataatc
    23881 tgaacctact attcctttag ctatttgagg aactataaga cctataaaaa taatattacc
    23941 agacactgac acagatgctc cagtcattaa tattacaagt actatacata taaatctaac
    24001 aaagttagtt ttttgtccta atcctattgc cacttcttct ccaagactta atatagttat
    24061 tcttggagcc ataataatag ctaaaataaa tccaatacca ccagctacaa atagcaattt
    24121 tataccttcc cacttcactc ctacaagccc tcctgatatc cagaaactta attgttgaga
    24181 taggttaaaa tacatagata ttgctgatgc caaagaaatt agcaatgtac caagagcgac
    24241 tcctgcaaga gctaatttta cattattaac tcttcctctt gatttagaac ttataaaata
    24301 tataaataat ccacttatac ttgctccaat aaatgcaaac atcattacac taaaacttcc
    24361 atgtataagt tgaggaagtt ttttttgaag taccagtgat atcgaaattg cgaatgtagc
    24421 tccttgtgta attcctataa cagatggttc agctatagga tttctcgtta ttccttgcat
    24481 tattgcccca gaaactgcta aaaatcctcc aacaagtgca gctgctattg cccttggaag
    24541 tctaacatcc tttactattc tcatatttat tccatcttcc atttttatca aactatctat
    24601 aacagttgat agattcatat cttttgcccc aatagttatg gataatacaa ttccaaatac
    24661 aagtacaact atacttacta atatcaacaa ataagttata ttttttttac tcagcttagt
    24721 caattatcct acctcctttt cccttttaat cgtcagtata atatcatcta ttaagtattt
    24781 taattttaag atttattaaa aataggtgtg aaaaatgaat atcattatct cacacctact
    24841 aagttaagaa ttactttgtt aattcatttt taactgattc taataataac tcttttccaa
    24901 ttggattgta tgattgactg aaaaatggag cagcatcaag tattgttaca ttaccatctt
    24961 ttactgctct tatttgagcc cagatagcac tattttctaa atctttttta tctgcttctg
    25021 tagcaattac aactatatgg tctgcatcta tatctgttaa accttccata cttaccattg
    25081 gtagtgttat tccatcctgt tttggcatat tcttaggttt agctaatttc atatcatcat
    25141 tgattagagt acctattcca ccatcactaa ataccataaa ttgacctgaa cttgccaaca
    25201 ctggtaaata agttttttct ccattctttt ctataacttc cttacctaac tttgtagctt
    25261 tttcatcata cttctgtagc caacttttag cttcttcttc tttgtcaaat aactttgaaa
    25321 catcagttaa cttgcttctc cagtcatttg cataatcttt catcataact acaggagcta
    25381 tttcttttaa ttggtcatat attttttctt gtctttgact cattattatt aaatcaggat
    25441 ttacttctaa tatagcttcc atatccatag tatccatcat tgagtgccca actatcttta
    25501 cacctttaag ctcttctctt atgtaacttg gtaatttatc agtttcatat gagtctacat
    25561 ttgctgttgc aacaggctta taacctgcta ataatagttc ttcactactt cctgatatat
    25621 caactatttt tttaggattt gatggtatct taacctctcc tttaacagat tgtacaactc
    25681 ttgtctctga tttatcttta tcctctgtat tcttatctga acatgctgta acaagtacta
    25741 acgtagttat aatagatata aatattgcaa gtgattttat ttttttcatg attacttctc
    25801 ctacttgttt attatactta catgatattg attatcataa gcacatattt attatagcat
    25861 ttttctattt ttagtcaata ttattttgta aaaggataaa aattcctaat taatcttaat
    25921 tatatatagc ttagataagg ttttcttata ttaaacagta tttttttaaa gaattggaca
    25981 caaatacaat gacttttcag ttaaacaatg ataaaatgca atttatagat aatgaattta
    26041 gtaaggagga ttcaaaacat ggtacattta gtatattgtg ataatgcagg aaaaaaagga
    26101 gaaaaggtct tagataaaat cttggctgga acaaaaacaa tggttattag aggtgctgct
    26161 ggtagaaaaa taccacatag tagagtattt gaagatgagt tactttattt tatggaaaaa
    26221 ggaactgcaa aaatatctgc aaaagccact gttaaaaagg tagaaaacta tgttaaatta
    26281 acagaagatg aaatttctaa aattttaaat gaaaatcaac caaaattaaa tttatctgaa
    26341 aagcaaaaag ttcgttggca taaaaaatgt ttatgcttag ttgaatttga aaatgttgaa
    26401 gaaataacac ctcttgattt tgaccatcaa ggaaatatgg atgattggct tattgttgaa
    26461 aaaattgaag atgtagttgt tggtactagt ataccttaca attatgagaa gtctaaattt
    26521 taaagtatca ataacataat actatattgc taaactctac ttaaataaaa aaagagtgca
    26581 tttaataatt ataaaaatta tttttataca ctctttttta ataataattt taaattactt
    26641 actttctaat ctttcatata catctataaa ttcttctgct aattgttgaa aattcatagc
    26701 tgtcattaaa tggtcttgtg catgaataag tataacactt atttcagttt tattaccatt
    26761 agcttcattt tgtatcaatt ctgtttggaa tttatgagct ttatgaattg attctttcga
    26821 cttttcaata cattctctag ctttatctat attcccgctc tttgcttctt ttattgcttc
    26881 aaatgctagc cctttcgcat cacctgcaaa tccaattatt ccaaaagata tctctatcat
    26941 cttctcatcc atttattcga cctcctaaat atctctatag cttgatatga ctaatccatt
    27001 cttttctaaa aattctttga cttttttaga tgtaagtatc ttgtgctctt tcgttctatc
    27061 aatagcataa gatgtagaat ttaaaatata atcatctaaa aaagctgggt gagacataat
    27121 atccactaca tcatacttca ttatttcgtc tatatttttt ataaaatatt cttcacttac
    27181 attttcctta taaaaactat ctattaaagg aactacttta gaatactcta aattatattc
    27241 aaatccacct cttattggaa gattatactt ggttacaatc ttctctatta ctggctttaa
    27301 acttaataaa gtatggatat gatggtgact atctaaatga gatatatcta ttccaagccc
    27361 ttttacccta tctatttgag cacaaagttc tctataaatc tcatcacaat ccattttttc
    27421 aataatttcg tctgtaatcc tcctaataaa aaaaccgtct ttatcaacaa tagtttttaa
    27481 gttagataat aatggtctac cacaacttaa agtcatatga actccacaat ttaaagtttt
    27541 attttcttta agtaagccta ctccatgttc taccccaggc atatttgcca tcatagaagt
    27601 agacctaact ataccattat tatgagctga aattattcca tagttcacag cttcacaata
    27661 tccaaaatca tctgcattta ttataatttt agtcatacca taacctactt tctatagaca
    27721 actatctatt aaatctagga agataatctt tatgagcttc caacatttcg tctaatatag
    27781 tttttgctaa gtcatcagat ggaatcaatg ggtttataca aagtgccaaa agagcagatt
    27841 cataatcgcc atatacagct gctttagctg ccaatacttc aaatgatttg atttgactta
    27901 ctaatccatg cacagaatct ggtaaatatc ctatacttat tggttttgga ccattttttg
    27961 ttattacact acttacttct accgcttgtt catctttaaa atctcttata gaacccttat
    28021 ttaatgtatt aactacttgg atatcttttt tgtcgttgta tatagaactt attaaattac
    28081 aagcagcatc actatagtaa gcccctcctc ttttttcaag ttgaggtggt tttacatcta
    28141 atttttcatc cttatataat tcaaataatt gttcttccaa ttctttaact acttgacctc
    28201 tagcttttcc ctctttaaac tctctcaact catcctcaag catttcttta gttttatagt
    28261 agtatctatg atatggacaa ggtattgcac ccaaagattt tacaaattca gcattaaaat
    28321 caattgcctt tatattttgc atacttatat ctgctttgac aaatttatca attgcttctt
    28381 ttgtaacatc ttctccatct aaagatacat ttagaccata aaccatatga ttaagacctg
    28441 caaaatccat acttattctg tcactttcta cattaaatag tttggcaaca tcatttttta
    28501 aatgaattgg cacgttgcat agacctatat atcttctaaa attagtatat ttgaatacag
    28561 cctctgttat tattcctgtt ggatttgtaa aattcaccat ccatgcattt ggacataatt
    28621 cttccacatc ttttattata tcaaatataa ctggtattgt gcgaagggct ttaaataaac
    28681 ctccagcacc attagtttcc tgccccataa ctccatgaga taaaggaata gtttcatctt
    28741 ttattctcgc atctaaaaga cctactctaa gttgtgttgt cacaaagtct gcatctctta
    28801 gagcttctct tctatctaaa gtaagattta ttttcatatc tactccagct ttttttacca
    28861 tcctcttagc tagatttcca actatctcta atttttcttt tccagcttct atatctacaa
    28921 gccataattc ctttacaggt aactcttcat atctatttat aaaaccctct acaagctcag
    28981 gagtataact tgaacctcct ccaattgtaa ctatttttag tccctttttc atctcattat
    29041 accatcctta cctataattt aatatactct acaaaacccc atacatttat ttaaaactct
    29101 atacatttat ttaccgaaat aaaactacct agttgttaat tttcaattta ttagcttcca
    29161 ttttttcaga cgctatgaca aatggaatat atattaatat tgataatatt aaattaaata
    29221 gtgctaaagc tccaccagtc cagtcacctg tagccattgc tccacctaat ataggaggtg
    29281 ttacccaagg tattttaggt aaaattactg gtgctactaa tccaaaatct attgctaagt
    29341 atgatacagc actacatatt actggtgcaa gtacaaatgg aatcataaat ataggattta
    29401 aaactattgg aagtccaaat atcaatggtt ctcctatgtt aaatattgca ggtggaccac
    29461 ctaatactat catctcttta cgtcttcttc cagctattaa taaagctact attaatccca
    29521 ataccattcc agaaccacct aaatatacaa atgcatcaaa gaatgcacct gcaagtacat
    29581 ggtatccttc cgttgctcct gcttgagcca aggcagcatt ttctccacct aatttcataa
    29641 gtatagtttc tgtaaaagga agtgctatat ttgcaccatg aagtccaaca gtccaaagta
    29701 tatgaattat aaatgcaata agcattgtag aacctagact atcagctaca tttgatagtg
    29761 gtgctcctaa ataagtattt aaaatatctg ttaagaaact tccattagaa agcattttta
    29821 taaatattcc tattacagta aatatcatta tagttaacat tcctggtatt aactttgcaa
    29881 atgatcttga aactgctggt ggaactccat ctggcatttt tatagttaat ttctttacct
    29941 tagataatct tacaaatatc tcagttgata ataatcctat taatatagaa gagaataaag
    30001 ctgctgtacc taagtaggtt tgttgtatca ttccccatcc ttcaactact gtagttcctc
    30061 cctcaggaac tattttacca atttgtggag ccattataaa aaagatagat aaagctataa
    30121 gcccagattg taacccatca ctctcataag attttgctag aaaataagca acacctatta
    30181 ctaagaatac tgccattgta cctattgctc cccaccaaag gtcgcctcca aatgtggtcc
    30241 agttttctcc aaatatactt gccatcaaat ttttgtatgc ttccaatggt aagttattta
    30301 tcaatgttcc caatgcacct accatagtta taggtatcat tactataaat gcatctctta
    30361 ctgctactaa gtgcctttga gctcctattt tagcagctac aggcactata tatttatcca
    30421 taaatgacat gaatttttcc attttacatc ctccagtaca actttatttt gaactattta
    30481 actctacagc tctatctaac acagcttttc cattacatct tccataatcc atcatattta
    30541 taacttctac tggaatatta tatggttttg ccatttcagt tgcttttcct aatacatatc
    30601 taacttgagg ccctaaaagt aagacatcac aattttctat ttcatttttt aagttcactt
    30661 ctgatatagc ccatattttt gcttctatac cgttttcctt tgctgcatcc tccatttttg
    30721 taactaataa actagttgac attcctgctg aacaagctaa cattattttt atcatttttt
    30781 cccctccaaa ttagattcat taaatatttt ttaagtcata agttttttaa actatacatt
    30841 taaaatatgt ttttctatta attttttctt aatgtatata tcttattttt aaatgtatat
    30901 acatttaatt cttgatatta ttatatacca aatgtatata catttcaaga aaactttttc
    30961 attttattta aattttattc gacaacagca ttcaaagtta atgtatatcc atagtgatat
    31021 gtgtgtgagt actcaaaaat ctcactattt tgtaacttac aaatttggct tatatataaa
    31081 agtggctctg gttcttctaa ttttagatta tccatacact cttcattcga tattacagct
    31141 tgtacttctc tctctgacat ggctatcttg tatccacaca cttcttcaac aaaattataa
    31201 agagaatttt cacaatgagt cttatttaaa tttggaaata acttaactgg catataagta
    31261 atcatatatg aaactcttac atcattagct aatcttactc tttttatttc ccaaacattt
    31321 tcttctaaac ttatattcaa cttacttgat aaattttcat ctgcctgaac tatttcaaga
    31381 gataccaata tattagatac tttatagcct ttactttcca tctctttagt aaatccactt
    31441 acagacgata tattatcata tatattattt gcaataacta tactacctac acctctttgt
    31501 ctagttatat agccttcttt aactagatta ttaagtgcag accttacagt cattctagtt
    31561 acattaaatt cttcactcag ctgtttttca gaagggatta aatccccttc tttcaaactg
    31621 tctgaattta ttaactctct aacatgattt tctataactt tataaattgg ctctttcatt
    31681 taattcactc cttattaaat cttttatata ataagataat tttatataca aacaaagaaa
    31741 atgtctatac atttaagata atttaaattt tattcagaaa tcttgtacaa ctttccgagt
    31801 tttaataaag atataaatag acttatcatt aatatacctt ttaatatact tgataaggtt
    31861 atactccacc atacaccatt tagacccaac atttctggtc tagatataaa gtaagccaat
    31921 ggtactcttg caccagtcag tatagtaatt ataatagaag gtatataggt tctccctaat
    31981 cctttgaaag ctcctgttgt tattatttct aaacacataa aaaattgaga atagcctaat
    32041 attttaagat aaactgctcc tttttctatt gcctcacttt catctataaa aatagaaaat
    32101 atactctctc ctgcaaacac taatactaaa gtagttatta taccaagtac agaagataca
    32161 gttatagcta ttttgcaacc tttatttatt ctagaatact tcttggcacc ataattttgt
    32221 ccaacaaaac tgcctaatgc aactgatagc ccttcagccg tcatccagga aattgcttct
    32281 atttgtgagc ctactttttg tactgcaaca gccacaggcc cccatgatgc tactattact
    32341 cctaatagca ttgagaatac tgtaaacata ccactttgta ttgctatagg taatcccaat
    32401 ttatacagaa ctttataata tttagtctct atttctttaa atagtcttaa tttaaaatat
    32461 tcttctttac ttttaactat tatatatata aaacatgtag taactactat ctgagcaaat
    32521 acagttgcaa tagcagctcc tgcaacacca agttttggag caaatcccca tccaaaaatt
    32581 aatatagggt caagtataat attagttatt aatcctactg tattaatacg aaaaggagtt
    32641 ttactatttc caagaccatt gaaaattgca gtaaatactg gattaataaa ataaaatacc
    32701 atacctagag ctactattat caaatactgt ctagacatgg ttataacttc taagtctcct
    32761 aatctaaaaa aacctatcaa ttgtttatta agtactatta aagaaattgt atatattatt
    32821 gctagtataa tatttatttc tattgctgac ttcatatatg atttagtagt gcttatatta
    32881 tttgcaccaa tactttgtgc tacttttatc tctccaccta cttttgaaat cataataaat
    32941 gccattgcaa gccatggaaa aaaaccagct gttccaactg cagctactgc tttacttccg
    33001 gctttaccaa cccatatcat atctatcatg ttatacccca tctgtataaa tgaagtcccc
    33061 attattggaa gtgatagctt aagtaatttt tctgtaattc ttccttctgt taaattaagt
    33121 ctgtctttca ttttccacct cttttatatt aaatttattt gcttatatag ctagattaat
    33181 cattttattt gattgagttc actatccttc aatttatatt atcatatctc aggattgcat
    33241 ctcaatcata cttatgttta agatacagta ttttacctat ctatgataaa attaatactt
    33301 atgaatataa atctagccat ttcatcaata catattctaa cctttaactt aacttcaatt
    33361 aagtatacat atcgtaacca attagaatat tttatacggt atcttccatt aataaacaac
    33421 caatatcaat gatttatata taattttatt tatacataaa atagtatact ataataataa
    33481 aaaaactata tttgaatgct ttaagcatgt ttactaattg taatttccac acaaatttga
    33541 taatatatga atatattatg gtgaaattta tagatatata ttaattatgg ggagattgtg
    33601 ggaggttctt ttcacaagac atttaagcta tgaaaataaa taaaaaattc aatagagttg
    33661 atatgcaggt ttcaatactt actgcaatta ttgtaataat atcttcctca tgtgtatatt
    33721 tttttaatta tacactaact tataagagca tgatatactc tttaagtgag aggtcaaaac
    33781 atatctatga atatgtagaa agaaatattg ataaaaatac ctttactgaa attaattcta
    33841 aagaagacca atcaaaaaag tcttataaaa gttctaaaat ccttttagaa tctgtaaaaa
    33901 atactactgg agtcatgtat ctatatactg ctaaaaaaac aaaagatgga tcactagtat
    33961 atgttgttga tggtttaaat tcatctagga gtgattttag aaatgctggt gatttgatag
    34021 aaaaagagat ttggggagac cttaacaaag cattagataa tcatatattg ttacctaaaa
    34081 agattaaatc aacatcttgg ggatacatat ttatatcgta ttttcctatt cacaatgatg
    34141 ttggtaaggt tgttggagta ataggaattg aatttgaagc aaaacatcaa tacaatacat
    34201 ttaaatttat aagtatagtt acaccaataa tagcattatt aacttgtcta atatcagcat
    34261 tgattgcagt gattctattt aaaagaatat ctaatcctac atatactgat tttgctaata
    34321 cagattattt aacaggccta aaaaatagaa atgcttttga aatagatatg aataacttaa
    34381 acaattcaaa tctaaagaat ctaatatcta tcatatcaat agatttagac ggtttaaaaa
    34441 agattaatga taaatttggc catcagacag gagacttata tattaagcaa gcaagtaaaa
    34501 ttatacaata tgtaataaat aaaaaatata tagtttatcg tattggaggt gatgaataca
    34561 taattattct aaaaaattta tctcacaaag aaataaataa tattataaat aatattaatt
    34621 taaaaatagt tgaagaaaac tcaactaatg aattaaaatt atcaatgtct attggatatg
    34681 ctatttttga tgaaaaagta gatgtttctt tatttgatac atatcgtcgt gcagattctc
    34741 aaatgtatga taacaaaagg aaaattaata aacaatatca ataataaatt taaaaagtct
    34801 attctttaaa taataaatca caagaataga cttttttaaa tttgtgtttt tataagataa
    34861 attatttaat taaagtttgt tcttttttct aaacgattct ttataaattc atatattact
    34921 ttcactaata tatttgtgac taatattaac acagctagtg atgctgcctg tgcagtactc
    34981 cccttatcat ctagttgtac aattgatatt gcagctactt ttgaacttgc agtatatata
    35041 aaaactaatg ctgatattgt caccatggag tttaggaaaa agtacataaa gttctctaaa
    35101 actgctggca aacaaagtgg tatagttacg ttcttaagaa ttgatgcgct gtttattccc
    35161 attgatttag ctaccatatc caattcttta tctactttct taatagcagt agtagctgtt
    35221 ataaatgcta ctgaaaagaa atgaactata ttacatataa ccattataag taatgttcca
    35281 tataatccat taaacaaatt cttaatgtat attgtatcca taaaattaaa ctctaattta
    35341 ttaaaaaata atacataagc tatacctaaa actaatcctg gcaatgccat aggcaatgta
    35401 cttaaaaaat acccaaatga ttttaaatat ggagcttttt ctgttttttc attcaaatat
    35461 gcaaataaga aaacaaagct cgtccctaga atagctgtca atagtgacat ttttattgaa
    35521 tttataaatg gtttaatccc atcaatactg tttttcatag aaaaatgttc cagtgtcagt
    35581 cttaaatcat atggccaaac tgatattaat gaagataaga ttacagttaa cattacagct
    35641 attataccaa atgcaataat tcctacaacc cctcctaaaa tactatctct taacttatta
    35701 gttttaacct tatatggaat tgactttgaa gttatatatg aattttgatt tttttcaatt
    35761 tttttagtta ctataaacga tattattgca ggtattaaaa gtaatattga tacagttgct
    35821 cccattggca tattctgttg ccctataatt tgcttatata catctattga taatacatta
    35881 aactctcctc ctatcacctt tggagctcca aaatctgtaa aacataatgt aaacgatacg
    35941 aatgcagaac ttattaaaga gtattttata cttggtattg ttatgcttat aaattctctt
    36001 atcttgctta tccccatagt attactagct tcatatagtc tataatcggt tgaacttaga
    36061 gatatataaa gtatttgaaa tgattgagga aatacatata taatttgtgc caatattatt
    36121 cccactgctc catatattgg aacatgcact ccaagtaaat tagttaccaa accttgattt
    36181 ccaaataaat atactagacc tattgcatgc atcattgttg gagcaaacat aggaaacata
    36241 gctaatgatt taaaaaaacc tttaaattta atctttgttc tagttaatgc atatgcatat
    36301 actaatgcaa gtgttagtga tattacagtt gatataactg atacaaatac tgtatttttt
    36361 agagaattta atagagattc agaagccaag tattcttgaa agttttgtaa tcctacaaag
    36421 actccatctt tatctaaaaa agcctgtaca aatagatata ttataggtgc taataaaaat
    36481 attactaaag acataactaa tactgtaagc atacattttt ttatatctaa attcattttt
    36541 gatttaagac ttatactttt agaacttata gcattattat tcatacactt atgctccttt
    36601 aacttttgga tactttaaat agtatttttc gtctatactt atttttacaa ggtcaccttc
    36661 tactaaattt aaattaaccc actcttttat agaagcatca cttattatga aattttcatt
    36721 taatttatct tttatttcaa ctctcaataa attccctcta aactctatat ttgatattat
    36781 tccttggtag ttttcttttg tcgatttctc tatttgaaca tattctggtc ttacagttaa
    36841 tatagaatct ccattatcaa aacagtttgt atctcctata aattgagctg taaataggtt
    36901 ttgaggattt tggtatattt cttctggagt ccctatttgc attatatctc ctccatttag
    36961 aatagctatt ttatctgcca tagttatagc ttcttcttgg tcatgtgtta ccattattgt
    37021 cgttatatta agttcttttt gtaatctttt aatgtccatt ctaagcttgt gtctaacctt
    37081 tgcatctaat gcggacattg gttcatctaa aagtaaaaac tttggttcaa gagctaatgc
    37141 tcttgctatt gcaatcctct gttgttgccc acctgataat gctttaggat atttatgagc
    37201 ttcattagtc aatccaactg tttcaagtac ttccttaact ttattatcta tttttccttt
    37261 tgaaactttt ctctctttta gtggaaaagc tatattgtta taagcagtca tattcggaaa
    37321 taatgcatat gattgaaata ctataccaaa acctctcttt gatggctcta aatttgtaat
    37381 atctttatcc tggagaatta tagttccgct atttacatct tcaagacctg ctattattct
    37441 aagtaatgta gttttaccac atccactagg tccgagtaaa catagaaatt ctccttcttc
    37501 aatatccaaa ctaatattat ttaaaaccct tttttggtca taacttttaa acacattatt
    37561 tatttttaag taactcatat cttatctctc cttatatagt tattttttta ttttaaattt
    37621 tatatcctac tttttcttct aaaattcttt catctaaatt attaatttaa actttactta
    37681 ctttcagatt ttgaaccgta ttttgattcc caagttttaa gtactgtact tctgtttttg
    37741 gcagcagagt ttaaatcaac ttcttttata agctgttcaa atggttgttt agaataacct
    37801 tttggaactg gattatctgt ctttatggaa gtaactgcta cttcatgtgc atatctatcc
    37861 atagctgaat cacttattgc ccaatctaaa aatagctttg cttctttttt tatattatct
    37921 tttttaacta aggcatttgc ttctaagtcc cagcctgaac cttcctttgg aaatacaacc
    37981 tctataggtt ctccactaga taattgttgt gtagctctat atccaagtgt aatacctatt
    38041 ggatactctc cagagccagc taacttcgca ggttttgaac ccgagtgtgt atatgtggct
    38101 atatttttat gtaatttatc catgtaattg taagcatctt tttcacccat catttgaata
    38161 agccctgata ctgtaagata ccccgttcca gatgatgctg gatttggcat tgttataagt
    38221 cctttatatt ctggttttat taaatcttca taactttttg gaatatctat acctagtttc
    38281 ttaagttcct tataattaac tactattgct gtctctggga cactcattcc tacccaagat
    38341 acatcatctt cagtatcttt aaatttttta tcaactttat cataattttt aggcttatat
    38401 gcttcaagtg caccatcttt ttttagctct atcatgttca ttgctgataa tccccatata
    38461 atatctgctt gtggattatc tttttctgct aatagttttg aagctattac accatgagaa
    38521 tccataacta tctctacatc tatattggga tactcttctc taaaaccttc catataataa
    38581 gctatctgct cttcctctag tgctgcatat attgtaagtt tattatttcc ttttccttga
    38641 gtagtttgtg gtgttgttga tatgcaactt gtcataaatg taatcactac aatgcacaga
    38701 caagtaaata taaattcttt catatcttcc ccctttgtgt tttttccttt ttttatttgt
    38761 tttttattgt ttttgcttat gtttatataa tacacctaat ttgttaatat aaagttaagt
    38821 gtaaattaac agttcgttaa tttttagaaa tttttgttaa atatattatt tttagttaaa
    38881 tacagtctgt ttttaaacaa tatattaatt tttatagtat ctacatttta atttaaacat
    38941 aaaaaataag tctaacctct ataaagtaga atgttagact tatttttata agtatttaat
    39001 tatacaacca aatcattttt tttcttttct tcaagtgctt ttttaagcac catatcctta
    39061 accttcataa gtctagtagt acttgctctc tcattttctt caagcttcat agctatatac
    39121 ttaatagtct caatgtaatt tggtatcatt acattttcaa gagcattaac acgtctacgt
    39181 gtcttttcta tctcttgtgc aagaagctgt gcactttttt cactttctgc aagtcttaaa
    39241 agtggttgca tagcatctga gaaagcttcc atagcagaat ctaattcacc tgatgtaaaa
    39301 gcaagaccat aaggataaat atcactttga ttgttttcag ttttaaagtc aaacacagga
    39361 acatcaacac tcattatatt tctcgtagaa acatctactg aaactgattg ctttggcatc
    39421 ataagtgctg aacccagata ctcctgactc ataactgctc ttgcgattat aaagttttta
    39481 taagcagtat caagagcatt ttctgcttct tcacgcaatc gcttattttc tcttacaatc
    39541 tcaagaaatt gtttcataag ttcatcaagt ttatctttaa gaagcttatg acctcttgta
    39601 gcagttttaa ggagtttctt aagtctagtc atttccatac gtgttggatt tatatttaat
    39661 ctagccacag atatcactcc ttaggcatat atttttcaaa atattcatca cggatacgtt
    39721 taagttcact ttttggtaac atactaagaa gcttccatcc gatttctaat gtctgctcaa
    39781 tagttctatc agttctaaat ccttgtgaaa catattcttt ctcaaattca tctgcaaact
    39841 tagcatacat taaatcaatc tcagaaagtg cagattcacc aagtatagcc ataagttctt
    39901 ttgcatcctt acctcttgaa taagctgcaa aaagctggtt cattgtatct gcatggtctt
    39961 cacgagtctt acctttacca atacctttat cttttagacg agaaagtgat ggtaaaacat
    40021 ctataggagg ctgtatatct tttttataaa gttcacggct aagtataatt tgcccttctg
    40081 taatgtatcc tgtaaggtca ggaattggat gtgtcttatc atcctcaggc attgtaagta
    40141 ttggaatcat agttatagac ccttcatgcc ctttcatttt tcctgctctt tcatacatag
    40201 tagcaaggtc agtataaaga tatcctggat atccacgacg tcctggaact tcttttttag
    40261 ctgctgaaac ttcacgcaac gcctctgcat agtttgtaat atccgttatt ataacaagta
    40321 catgcatatc ttgctcgaaa gcaaggtatt ctgctgctgt aagtgccatt ctaggtgtag
    40381 atacacgctc tacagctggg tcatttgcaa ggtttacaaa cactacagaa cggtcgattg
    40441 caccagttgc tttaaaatcg cttataaaaa attcagcatc ttcaaaagtt ataccaactg
    40501 cagcaaatac aacggcgaac ttagaatcag ttccacgtac ttttgcctgt cttgcaatct
    40561 gaactgcaag attggcatgt ggcaaaccag accctgagaa tataggaagt ttttgccctc
    40621 taaccaatgt attaagtcca tctatagcag agactccagt ttgtataaac tcggccggat
    40681 aatcacgtgc tgctggattt attggagctc catttatatc aagtctttta tctggtataa
    40741 tttctggacc tccatcaata ggtctaccca ttccactaaa aacacgacct aatatatctg
    40801 gtgaaactcc aaagtcaaga ctctttccta aaaaacgaac tttactttca gcaaggttta
    40861 tacctgttga gctttcgaaa agctgaacaa gtgcagtatc tccatttacc tctagaactt
    40921 tgcaacggcg aatttctcca tttgaaagtt caatttctcc aagttcatca aattttacac
    40981 cttctacgcc gtttattaac ataagaggac cggctacctc ttgtattgac ttatattcct
    41041 ttatcatagt tcttcagccc ccctcttttt aataaggtct gcaagctcgt tatctatttc
    41101 atcttcaatt gaatcgtaag ctgcatctat atcgccttct tctacatact tagcacgacc
    41161 aatcttttca agaactgaaa gcttgataat atcgtttata gtaacatttt gcttgatagc
    41221 atcttgtgct gacttataga attttaatat aagtcccatc attctatatt gtttatgaag
    41281 tgatgtataa gtatcaactt catggaaaga gttttgatga aggaagtctt ctcttataga
    41341 acgagtaacc tctagtatca atctatctcc atctgaaagt gaatcaaccc caaccaattg
    41401 aactatttct tgaagttcag attcaacttg taaaagtttc atagcttgag ctctttgagc
    41461 tgagaaatta tcatttacat ttttatctgc ccatacatca actttttcct gatagagtga
    41521 ataactagtc aaccagttaa ttgctgggaa atgacgttta tatgctaaag atgcatcaag
    41581 accccagaat acttttacta tacgaagagt tgcttggctg acaggttctg atgtatcacc
    41641 accaggagga gaaactgcac caattgctgt aagtgttcct tctctttcat ccatacctag
    41701 acaatttacc tttcctgctc tctcatagaa ttgagcaaga cgtgaaccta agtatgcagg
    41761 ataaccttct tcaccaggca tctcctctaa acgaccactc atctctctaa gagcctcagc
    41821 ccatcttgat gtagagtctg ccataagtgc aacactatat cccatatctc taaaatattc
    41881 agctattgta ataccagtgt atatagaagc ttcacgtgca gcaaccggca tatctgatgt
    41941 atttgctata agcacagttc tttgcataag tgactcgcct gttcttgggt cttttaattc
    42001 aggaaattca agaagaacat ctgtcatttc atttccacgc tcgccacagc ctatatatac
    42061 aactatatct gcatctgccc atttagcaag ctgatgctgt gtaacagttt ttccacttcc
    42121 aaaaggtccc ggtatagctg ctaccccacc ttttgtgatt gggaaaaatg tatctataac
    42181 tctttgacct gtaacaagaa gtgaatctgg tgttttcttt tctctatatg gtctttcttt
    42241 tctaactggc catttttgca tcatagttac aggtatatca ttaccttttt catctgtaat
    42301 aacacaaact gtatcttcaa cagtaaattc accagaatat atttctttta tagtaccctt
    42361 aactccataa ggaaccataa ttttacattc tactactgct gtttcttgaa cgccaccaat
    42421 tatatctcct gctacaacct tatcaccaac agatttttta ggtttaaatt cccacttagt
    42481 tgttctatca agtgaagata cttcaacacc tttagttata ttagtacctg aaatatcata
    42541 cattttttca agtggacgtt gaataccatc ataaatagtt gaaataagac ctggtccaag
    42601 ttcaacactc ataggcattc caagagaaac tacttcttcc cctggtccta gaccggaagt
    42661 ttcctcataa acctgtatag aagctttatc tccatgcatt tctataatct cgcctataag
    42721 atgtttatct gaaactctta cgacgtcaaa catattcgca tctctcatgc cctccgctac
    42781 aacaagcggt cctgacacct ttacaattgt gcctgttttc atattctctc acctgcaatc
    42841 atatacttta tcaaaattta aatattccct cctatatttt attagcacat atatttagca
    42901 ccaataaaat ttgagattgt cttttattca actttcatct attctttaaa taatatatct
    42961 gcacctatag cacgtttact agattctctt acttcattca tacctaatcc catacttcct
    43021 ccaatacctg gaataactat aatggcagga agttggtaat ctttgtattt tgctattgat
    43081 tcttttgcaa gcaatgatgc ctgttctgta atataaatta tcgcatattc atcttcaacc
    43141 aacttatgta tacttttttt aatttcgtca ctatcatagg ctgggaagat atctattcca
    43201 agagctaaaa atcccatgat gctatcttta tctccaacta ctcctacctt atacataagc
    43261 atcccttagc ctttctttta tagtatctgc gtctatatta ttaagcttac tagtcaaaat
    43321 aattctaatt gtttttatct ctgattcttt agcatataaa tatgcaatta gtggttctaa
    43381 tgtaagaggc ttaaattttg ctcctctaac atattccata agatagtcat cacaaagttt
    43441 ttcaaagaca gtaaaaccac tatccatgcc atttttacag acatcagagt aagaagtaga
    43501 tttaaaacat gatgctggag tatctgagga aaaagcttct aagaattttt ccttatcaag
    43561 tgagccacct gaaacaaaca cattcataaa catatcaaaa tctttcttca tatttttgac
    43621 tcttataaaa ctctttagat ttgttaaatc acaaactttc ataacataat ctattacaaa
    43681 tttgttttgg ctttctttag cagtttcttt catactggta aaggttgctt tatctagaac
    43741 aatatcaacc atctggccat tttgtgtctt attataaaca tcaaatgcct ctaatatagc
    43801 tgatgacatt atcttaggta aatcactaaa actacgattt gctaaagatt ctctaagctt
    43861 aataagagga attgtacctc catcaattaa atatttctca ccatctacac cagaaatttc
    43921 ttctttaatc agcactttta aattgtgata gtcattttta taaagaaata catcaacaaa
    43981 tttttcttca ggagctagac ttttaagaat ttcatatgtc tttgataatt cttgagtcaa
    44041 aatattctca aaattatgta tattattatt tgatgtatca gcataacctg cctcagctat
    44101 aattcttaaa gagtcttcag ggcttttggc ttctgccatt tgaataaaca tttgtctgtt
    44161 aagaagctgt ttttcaagca ctcttattcg tgcaacggca tagggataag ttttttcttc
    44221 tgccattttt taacccctcc ttaattaaac aaaatttctg ctgcaatttg ttcaatatat
    44281 tcatgctcta ctgcaattat ggcttcaaat gaataattgt actcaatgtc acctttttta
    44341 actatgaaac ctccagtaat atctctagtt tcatcagaca ctttaatgcc tttttttgat
    44401 agcacatctt ttaatgtttt tttatctttt ttagataata ctatctctga atcatctgta
    44461 caatttgatt tttcaatcat gtttagtata atctcttcat attcattatc tttaagattt
    44521 aaaagtttgt ttttagcttc taaaattacc tcttctaaaa tctcttgttt ttttgataat
    44581 atttgctttt tagcctccat ataagctcct gatatttcct tagaagccgc tttttctgct
    44641 tctgcctctg caagctttgt ataggaagcc atttcctttt cagctttttc attagctgaa
    44701 tttactttta aatctgcttc acttttagct ttatccaata tttcttgtgc ttcttgtttg
    44761 gcatcagcta taattctgtc aatcattttt tgttcattac ccataggagt gcttcctcct
    44821 tttcattttt tttacttatg aattataatt tataattaac caattgcaat tccattgtaa
    44881 gctaagaaag aaacaagtag tcctaaaaga gcatatgttt caactattgc tgcaaatgtt
    44941 atagctttac caagttcttc aggtcttttt gcaactattc caactcctgc tgctgctgct
    45001 ttaccttgtg ctatacctga tactaaacca actaaaccaa ttggaagtcc tgccataaga
    45061 agttgtaaac cttgagctga tgttggtatt gcatctccac caataattcc tgcttttgat
    45121 aaaataagga aagctatgat aaatccatac agaccttgtg taccaggaag aagttgtaaa
    45181 actagaagtt gaccgaattt actagggtct tctgttacaa cacctgctgc ggcttgacct
    45241 gctattgaag tacctttagc agaacccata cctgcaaata atgctgctat agcagctcct
    45301 gatattgcca aaaattgacc atttcctaac gcgtttaaaa attccatatt tacatctcct
    45361 ttttattaat gcttgtatat tttgttttat acttaaacgg aacaaaagga actcctccgc
    45421 cttcataaaa cttattgaaa aattctacat actgtaacct acttgtatga acatatgcac
    45481 ctagtgcatt tataagaagg tttattgtgt gtcctacaac accaattaca actgcaagta
    45541 tcggaccagc tatagctcct aaaaggttga taacttgtgc tataactcct gttgttaaac
    45601 aaagcgccat aattcttgta taagataata tatcagcaaa ataacttgtt ataccataga
    45661 gacttgaaaa tcctttaaaa agcttaacag gaataccttt aaagcttctt ccttgagtaa
    45721 gtacaagacc tatagcacct ataattgcta aatattttcc tacttctgaa aatacaccta
    45781 tatcccctgc aacaaaaggt attataagta gacatacacc tgttatacac aaataccaaa
    45841 aacctatgtc aaagattgca tctattacct ttccacttct tattaatgta tatgctttta
    45901 ttcccaaacc tacatatata tggataatac cgaacagtaa cgacattccc ataagtgtca
    45961 taacatcctc taaaggatta attaatgctt ttataggaat taaatccccc aaaatacccc
    46021 cgaatataag accccataca gtagttgaaa ctccacagat accaataagt ttaattaaat
    46081 ttccttcacc tttttgtatc tttaatttat atacaacaaa tccacagact actgcaatta
    46141 ttaacccata tgcggcgtca cttagcatca ttccaaagaa tacaacaaag aagaaagtta
    46201 aaattgtatt tgggtcaata tcttttgtac ttggatagct gtacatgtta gttacacttt
    46261 caaaaggtgt tactaattta ttgttttcaa gcagaattgg gaagccttct tctttacttc
    46321 cttcttcagt ctgaacacaa caattaaact tttctgtaat ttctttaatt aattcatctg
    46381 cccttttgct tggtaaccaa ccattaagac agaatgttgt ttttgttttg acaagtctct
    46441 ctataatctt tttttggtct ctttctatag tgtaaaaatc atataggttt tcaagcatgt
    46501 gtatactatc tgcatgtctt ttaatttcct ctgttttgtc cttagaaacg ttttcctgtc
    46561 taattatcat ttcattgtat ttcttgatag cttgtaacgg tgtaccttct tcatttggta
    46621 atgaaaccac tgaaaaatta aattctttta aaacttctaa aacatcatca aaagtttctt
    46681 ttaaagtaat taaataaaca tagttcatta acttgtcaga attgacaatc ccaataacac
    46741 tttctggtat ttcatcttct aattttactc ttaaagcatc aatgtttgcg cttatgggca
    46801 cagttccaag tatagttttt gtatacttag tttccatatt ttcaagagga atatcaaagt
    46861 tcacccatgg ttctaacata cttttattgt tgttaagtaa gtttatgtta gaccttattg
    46921 tacctatctc cttgtgcaaa ttgtttaact ccagagcttc agcataaact tgcccttctt
    46981 tttcactatc aattttgtta aactctcttt ttggtgcaag tagggatttt ttttgtttca
    47041 ctagtgaaga aacaatatta attgcttggt ttacatgaga cattttctgc tctagcatta
    47101 taacttcaga ttcttcacta tcttttacaa gataattttt taattcctcc tcctcagtaa
    47161 gaaaggcact atcatcaatc tgaacaaacc cacggttcat aagtagcttt aaaatctggc
    47221 ttttttgagc ttctaagcca acaatagata ttttgttcat attaactatt gccatattat
    47281 ctcactacct ttccaataac cgcttcaata gccttacttt ttctttcttc agcagttttt
    47341 tcaattttca gacattcact tttagcatta gctaagattt tatcaacctc tgctctactg
    47401 tcggtgactg ccttctcaat aagagtttct tccataagct ttgcttgttt ttttacatca
    47461 gcaaatatgc tttcacattt gtttacagca tcagctctga tagactctgc atcagtaaca
    47521 gcttttactt tgatactgtc tgctgtctgc tctgcctcaa caatgctttt tattatttca
    47581 aaagccatcc aaatcacctc tcttaccttt ttactatgta tataaccaat tagatttttt
    47641 tattctaatt agattactac ctcattgtta aagtataaac agcattttta tataataatt
    47701 aatatttttc aacgtttatt tcttaataaa acatatttta gaatagtact tgcctagatt
    47761 tacatccttt tttgacttac ttttagacta tgacatgagt atggttttta aataccatta
    47821 ttttcaataa ttataattta aagcgatata gtttgtcaat agcgttattt tgttttaaaa
    47881 ttaacgttat gtcgacattt taaattttta ttttattttt tttataattt taatttaatt
    47941 tattacatat aaaagttttt tatattcttt ttcaacctct aaccattgaa gtttattgtt
    48001 ttttttgaat ttttagaaat atctcgcact attttatcta acttttataa atcatcaagc
    48061 tatatcgatt tttgtcacct tatttttaat ctaatttttt tatatctagt tagaaataaa
    48121 tcaatatttt taaagattga taaaacttac acctagttct aatagtttta ttttaaggca
    48181 atgtattaat cttaagtaga taatatcatt agttactcta tatattcgac aacattataa
    48241 aaaaatcctc gtaccaatca ctccaatctt caataggata tgctgctcct ccatttacac
    48301 ttttcagtag attggatgta tattcttctc ccatagaaaa aagttcccct actgtcatat
    48361 cataacctct agatgtaaca accttacaaa atgaatccat aacatcacgt tgttctcttg
    48421 tttttataag ttcttttcta aattcatcat cattcttagc ttcttctaaa attttagtta
    48481 atttttcatt cattatgtat ttctcctttt aagtagtctt tctgtaaact tcaataagct
    48541 ttatataaaa aggaagccat cctaaataaa ctaagatgac ttcgcttaac tgttagcaac
    48601 acatttacta gtataaaata attggtgtac ctggttctat attttcatat attttctttg
    48661 ccttagaaaa aggtgtattt acacatccat gtgaacctga agacttatat atacttccac
    48721 caaaagaatt tctccaagta gcatcatgaa tgcctatgtt cccattaaac ggaagccaat
    48781 aacttacagg tgaactgtaa ccttgaccag ttaatgaaac atttctagcc ttataattaa
    48841 gaggatatat acctgatggt gtagcaaatc ctctagctaa atcacctgtt acaacgtctg
    48901 ttgatacaag catttctcca tttttaaaga accacatatg ttggccagaa agactaatct
    48961 ctacatatgt atttccaata tcatctttat tacgagatac agctttttgt atgtaaatag
    49021 gttctcttgt aacattttta ccagctttta caacatcaac taatgcttca gcttctttct
    49081 ttttatctat caaccaacca taaattccac ctgaaattgt aatatcttta cctgtggcag
    49141 ttttaaatgg tcttgagtca ccaaaagtac tgtatttttt acttaaacct cttgtatatt
    49201 ctaccatagc atcattatca aaagttatgt cataattctt ttctgagtct acttttaacc
    49261 atttattaat ttcagaacca tctaaagttt ccttacggtc actaaaatca tatgtaacga
    49321 ctacaccaac atacttatct aatgtcttaa tagcttgttt caacttttct gaatcctttg
    49381 tgttgtttgg tgcttcataa actttacttt catccagaga aatagaagta ttcccagaat
    49441 taattgagtt ttgtactaac ttataaaaat catctttttt cactttgttt ccaagtacct
    49501 ctggtactat aacataagcg tttttagtgc tatcatactt aggataagca ctcttaggtt
    49561 ctacaacatt tttactatca aaacaagata aagcattaaa ccttttttct agtaaatctt
    49621 tattatatga aagagttatc atattattat actctttaga cttaaatatt ccaccaatcc
    49681 atccaatagc gctctgacta tcttttatct tttgaacttt atcatctggt gtaaatttta
    49741 aatcaatatc ttttgctttt atttgttctt tcttattgtt tctttcctct aagtttaaag
    49801 tataggaatt agtactagat gttaacaaat catttacttg ctcaactgtt tttcctgaag
    49861 cctttacgcc attaatagtc gttccaaagt aaaaatgatt tctaaaaaag attgccattc
    49921 ctatataagc tacaaacaaa acacctacta ctatagctat cttaaatttt agattttttt
    49981 gtccactttc ttgttttacc tcttttccat caatcataat taaatccttc cttacattgt
    50041 aattacctat gtaccttatt ggtttttaaa caaaaccatg tatgccttat tttatatacc
    50101 caaattactg tatcattatt tatattttta aatatatagt tatttctata aaaccatttt
    50161 atcttttaga taaaatgatt attataaatc ccccctttca aaatccctta aacacaatct
    50221 ctgctatatt aatatattac catactttta ataatcgtgg gactatctat ctatttttgt
    50281 aacactaaac tactatttta aaaaagaaac taattttgtg ttagtttctt tatcttaact
    50341 tatttttata ttgaatttta gcttaacata acgttgttat atagattcta tttaattatt
    50401 ttagtttcta tatcttttcc atctgcatat tctttaagta attccttagc atatccaaaa
    50461 tattcttcaa tatcagattc ttgactaaaa gaaaatatac aaattaatat ttcatttgca
    50521 tcttctgtta tcattgctct ttcagaatca ctagttggac tcccaaattt acccactgaa
    50581 tcagataata taggaagatt ttctatattt attaattctt tcccaatacc cttatactgt
    50641 tctccttcat ctcctacagt aaaacatact ggtgaatgta atttgcttac attatatgta
    50701 cctactggat ataatgattt tattgatatt aaattgttta tttctactat attatttatt
    50761 gtataaagac ccttcccttg aataattctt ctaaccaatg cttctgaaga tgttctatat
    50821 ctacttggat tttttccaag ctctttataa gcatttcttg catctttaat tttatctagt
    50881 ttcgtaatgt cttcaacttt tagactttga tttatagtat cacaaaactc atttatttca
    50941 ttaattaatt tttctgagct tttatttact gctacttttg cttgaatgca acccagctta
    51001 gcattaggcc atctttcctt aagtttgtca tcaatactta tatctatcat acttaaacaa
    51061 ctcctataca ctaaattttt attaaagttt ttatcatcta tacattatag tactattctt
    51121 ttgttaataa catgtttatt agcaaaataa atttattttt aatataattt ttataaaata
    51181 caattatttt ttgctttata ttagacaata cattgataaa ataaaaaaac actaattatt
    51241 tagcaatgat ttattaaaat caagcattca taatagtgct ttatataaaa atcaaatttt
    51301 gttttaaagt aaatctaaaa aattcttagc tggcattggt cgtgcaaaat aaaatccttg
    51361 aactaaatca catccaaatt gtttcattaa ctcagcttgt tctttactct caacaccttc
    51421 tataattaca actaaatcta gtgattttgc tagagacact attgattttg ctatattgcg
    51481 acttctctca tcatgttcaa tgccaccaag aaaagactta tctagcttca atatatcaat
    51541 tggcatatct cttaaacaac taatagaaga atatcctgta ccaaagtcat ccatagaaat
    51601 agaaaatcca tacgtcctca actcatttaa aacattcttt aaaatattta actcattaat
    51661 agccacagtt tctgttatct ctatttcaat gaaatctgaa cttaaattat acatttttat
    51721 tgttttattg ataaatttta caatatctgg ttgatacaac tcgaccctag ataaatttac
    51781 tgaaactgga actatctttt taccttgctt tttccattca tataaatcat tgcaaaccct
    51841 ttcaaataca aatctcccaa tttttaaaat caacttactt ttctctgcta ttggaataaa
    51901 tactgcggga cttataaaac catgttctgg atgattccat cttattagtg cttcactccc
    51961 tatcatttct ccagttacaa gtgaaaactt aggttgataa tacacttcaa attgattttt
    52021 taccaatgct atttttatat catctaaaat cattgattcc tcactaagct tattcctaac
    52081 atcttcatta taaatagcat aatttatatt tttaccttta gcaacagacc tagccatatt
    52141 tgctttattt acagcctttt gaatatctac ttcaccttct tcaacaaaat aaatacccac
    52201 acacagagaa atttcaaaga ctatactcaa attactttca atattacttc gtatattatc
    52261 aagttttctt ataattttat ttcttccatt tttataatca cagaaaattg caaaataatc
    52321 acttgaaagt cgtgcatata cctcaccttc tgttaattct tcttttaaag cttttgctat
    52381 tgtaattaat aaattgtcac caaattcata tccaaaagta tcattaatca tcttaaagtt
    52441 attaatatca aaatatacta taatataatt taaagatggt ttatctaaaa gatacttact
    52501 acattctcta taaaatttat tttgatttcc tatatatgta actttatctt cataagccaa
    52561 acttaatatt tctttttcat tagatttttt tatataaatt atataaaaaa taattgcaat
    52621 aaatattaat acaatagata acactgcata tacagctctg ttgattattt gttttgaatt
    52681 actaaataca acagtttctg gaattgaaaa aattaaattc cagtctttta tataattatt
    52741 ttcactattc ccattatcca tagtggcata tcctaaatat cttctttctt ccaacatatt
    52801 taatacagtt gcacctgttt tattttcttg caattctttt tttaatctat ttacttcttg
    52861 aatatcatta tcctgtttta acaatttata aatatttttt gataggctat ccccttgtgg
    52921 atgaaggata attgtaccat tattttttac aacataagat atactattat tttcataaaa
    52981 agatatatta ataagtttca tcagagtatt gatattgtaa gtacaatata aaactccaac
    53041 tactttatca ttatttaata atggtactga aatagcaatc ttctttatag acttatttac
    53101 ttcatcaaca tatggacttg aaacatactt cttaccttta attgagtttt tgaaaaattc
    53161 tctatctttc actgaaaact tatcaccttt ggtattttca gcattaccat ttatatcaac
    53221 tattgccata gtgtaaaatt tttgattttc catttcatct aaaagatttt tcattttttc
    53281 ctcattaggt atatctggat ttgaagcata atagttagaa aaaatattta aaacatctaa
    53341 atctttttca atttgtcttt gtgctagttg agagccttgt actgcaactt cttttatatg
    53401 tatttttgcc tctgacataa gaagatgttt tgtatcttct atatagatat accccattgt
    53461 caaaaataat gatgttaaaa aaagaattgt aattaatgta aaacttttat ttctccctgt
    53521 cattcgactc ctcccctaat tagtcaatac ttcatataga taatatatcc tttttatacc
    53581 aattcatcat ttatattgta tataactatt cttaaatgtt acacataaaa tcaacataat
    53641 aaccataaaa acattctttt attattataa taacactatt taattgaatt ttaaattaaa
    53701 gaataaaaac tatccatttt ctcaaacaaa aaaactctta tacaataaaa ctttgtttta
    53761 tgtataagag tttttatttt atctatttta tagcaaactt atcttccata atatgaattt
    53821 aaatacatat cttttatttc attcataagt ggatatcttg gatttgctcc tgtacattgg
    53881 tcatcaaatg cttgaattac catttcatct attgaatcca taaacttaga ttcttcaacg
    53941 ccaaactctt ttatagattt aggaatacca actttaagtt ttaaatcttc aatagctacc
    54001 aataaacttt ggaatttctc ttcatcagaa tttcctttta atcctaaaaa tgaagctatt
    54061 tcagcatatc tctttaatgc ttctggatat ttatattgag agaatgctcc cattttcttt
    54121 ggtgcatctg aagaattaaa tttcataact tctgttatca aaagagcatt tgcaacacca
    54181 tgtggtacat ggtggaaagc acctaattta tgagccatag agtgacaaac acccaaaaat
    54241 gcatttgcaa aagccatacc tgccattgta gaagcatttg ccattttttc tctagcttct
    54301 gggtcttttg caccattatc atatgcacgt ggaagatatt caaatatact cttaatagct
    54361 tgtaaagcta atccatttgt ataatcggtt gcaagcatag atacatatgc ttctaatgca
    54421 tgagttaaag catctatacc agaagcagat gttaagcttt ttggcatatt catcatcata
    54481 tcagaatcaa ctatagccat atttggcatc agttcataat ctgctaatgg atattttact
    54541 cctgtatctt ggtcagttat aacagcaaat ggtgttactt ctgaaccagt acctgctgat
    54601 gttggtatag ctacaaaatt agctttttca cccattttag ggaatgtata tacccttttt
    54661 cttatatcca taaatctcat agctaggtct tggaaatcta catctggatg ctcatacaat
    54721 gtccacatta tctttcctgc atccattgca cttcctccac ctatagatat tattacatct
    54781 ggattaaatt ctctcatagc tttagcgcct atcttagcac attccaaagt tgggtctggt
    54841 tctacttcaa agaatgtagt gtacttaata ttcatttcat ctaataaatc agtaactgct
    54901 ttagtatatc cattattgta aaggaatgta tctgttacaa taaaagctct cttcttattc
    54961 attacatctt taagttcttt taatgctact cctaaacaac cttttttgaa ataaactttt
    55021 tctggtgctc taaaccaaag catattttct ctcctctcag ctactgtttt aatattaagt
    55081 aaatgcttaa ctcctacatt ttcagatact gaatttccac cccaagaccc acatcctaaa
    55141 gtaagagaag gagctagttt aaaattatat aagtccccta taccaccttg agaagaagga
    55201 gtattaatta aaattctaca agttttcatg gctgaagtaa acttatcaag tttttctctt
    55261 tgatttacat catcaatata taatgaagat gtatgcccaa atccaccatc ttctactaat
    55321 ttttctgctt ttctaactgc atcatcaaaa tcacttgcct tgtacattgc aagtactggt
    55381 gaaagtttct catgagcaaa agcttcttct atttcaactg attctacttc acctattaaa
    55441 accttagtat ctactgggac ttcaaagcct gcaagttttg ctatagtaca agctgtttgc
    55501 ccaactattt tagaatttaa tcctccattt actaaaatta tatttcttat tttttctgtt
    55561 tcatctttat ctaataaata agcaccacgc tctttaaatt cttttttgac ttcattgtat
    55621 atattttcta atactataac cgattgttca gaagcacata tcataccatt gtcaaaagtc
    55681 ttagatacaa gaattgagtt tacagcagtt tttatatctg cactatcatc aattattgct
    55741 ggagtattac ctgcaccaac tccaatagct ggttttccag atgaatatgc tgatttaacc
    55801 attccaggac ctcctgtagc aagggtcaaa tccacttctt tcataacagt tgtagttaat
    55861 tctaaagatg gctcatctat ccatcctatt ataccttcag gagctccagc taaaacagca
    55921 gcctcaagaa caacttttgc agcctctata gtagaatttt ttgctcttgg atgtggagag
    55981 aaaattattc catttctagt ttttaaagct aaaagtgctt taaagatagc agtagaagtt
    56041 ggattagttg taggtacaac tgctgcaata acacctattg gttcagcaat tttagtcata
    56101 ccaaatgctt cgtctttctc tataacacca caagttttag tttctttata tgcattatat
    56161 atatactctg aggcataatg attctttata accttatctt cagcaattcc cataccagtt
    56221 tctttttgtg ccattatagc tagaggaact ctttgcttat tagctgccaa agatgctgct
    56281 aaaaaaattt tatctacctg ctcttgacta tatgtagcaa atattttttg agcttctctg
    56341 atttttgcta gttttttaac taagctttca acgttattta cagtatctaa accaactgac
    56401 ataacttcat tttctttttt attttctatt attacttgtt ctttatgagc cattttaaca
    56461 acctcctgag atagtttttt atttattatg ttaatttatt aacattatag catttacttt
    56521 tcctattttc aagttatttt tttaacattt tattttttat ctaatataac atcatttcta
    56581 ctaataatat ctagctttac aaatacttaa tttaactaga tatcacttta ttttttacgc
    56641 aaatcactaa tgaaatcatc taaaaactta aggataacgt tattttttta acgtttatat
    56701 ttttgtattt tttgcaaata aaactttata aaaagaattt ttaatatgat actctatcaa
    56761 gtttaaaaat aaaaatcctt gaaaatgaaa attaaaactt aattaacaag ttaaagtaaa
    56821 tatagttaaa tgtacaaaca tttacaacat atagtataat taaaaaaata tatattcatg
    56881 atttttatat aatatgaaat aaaatatatt taatattata tagataaatt tgaagtttat
    56941 aagaaggagg ataagcatgg aaaaattaaa aacaattagc gttttttctt tgattataag
    57001 tgtaatttta acgattggag gtattggaat agtaacttat tatgtagata atttgtttat
    57061 tagaggacta tcagtatttg ttttaattat gtcctcttct tttgtatcaa caacagttag
    57121 attgatattt gaagagtcaa aacgatataa attctaattt aaaaattgga ttaagtaaat
    57181 aattttaata tagcaaaaat tacggagatt agctcaaaat agacttttta gtccatgagg
    57241 catttttttg tatgaaatta aatatatttt catcatttca acaagaaaaa gaggcttctc
    57301 tctattttga gacagcctct ttgctagtat tgtatctaat acataatatt ataagaacca
    57361 attctattga tgtggacttt ttataacagg ctgtagctgt ttgttctcta gagcaagttc
    57421 tatagttgga attattagct cagtatgtgc aatcatagaa ttaggctttg ctttgcaatt
    57481 atcttgacca aaaggtacaa aatatatatt tttagaatta aggagaatgc caatattttt
    57541 gaaattaaag cttagagcat cattcgttga tattgatatt actagtggtt tatcattacg
    57601 aagatgacct tttgctgcca ttaatactgg tgaatctgta ataccatttg ctaattttgc
    57661 agcagtattt ccagtacaag gagctattat tataatatca gcaattcctt taggtccaaa
    57721 tggctctgcc tcttcaatcg taactatagg tttatttcct gtaagttcat atgctttttt
    57781 cataaactct tcagaatttc caaatctaca atcgatattt tgtgatacat ctgaaaatat
    57841 agtgtgtaca ttagctcctt ctttaaccaa attctctaac tctataaata ttttatcata
    57901 agtacaaaac gaaccagtaa atcctactcc aattgtaagt ccttcaagtc tcatttctaa
    57961 tcccttcttt catttataat attttctata gcgttaacaa gaatttcacc agatgtcttt
    58021 ggtgaatatt taccaggaag cccaaggcaa agagtcgcat taatccctgc ttctttaaca
    58081 acagaataat ctactcctcc aggagaagaa gctatatcta taatagttac ttcttgtgat
    58141 actttttcta acctttcttt cgttaaaata cactctggta tggtgttaaa aatataatca
    58201 aattgtaaaa gtttttcatc aagttgactt aaagaaatac tggaatatcc aaatgcatta
    58261 gccgtacttc ttgcatcatt atttcttgcg ccaacacata catttgcatc caaagcgtat
    58321 aatttttttg caagtatttg tgcacatctt ccataaccta atactaaaca gttactttga
    58381 tgtatattga ttatacttct tttaatagct tctgctatag ccccttcagc tgtagcaact
    58441 gcatttaaaa tagcaacatc attcattttc ataaaatcat aaatctgaat agagtgttgc
    58501 tcgcaaaatt tacaaacttt agttggtatg ttacctccaa ataatatttg attaggagat
    58561 aaattaatta gcaagttatc aatagtagca tcaagacaat catccgtatt ttcaatatta
    58621 acttgattct ttgtaaatgg aattggacat aaaataatgg aacttttgga tagtgcttca
    58681 gctagtgaag ttgattttcc aacgattcca tttataattt catttgatat cccataagtg
    58741 actacagaat accctttatt aattagtttt tttaccatgt acacttgtcg caggtctccc
    58801 ccaataacag ttatatcgta ttgatttgac atattgtaag cctcctttac tatatacaat
    58861 atgtttttat caatacattg gttcactaaa attactaaat attatactta taacattttt
    58921 aataatgcta atctatctat cttttcagaa gaggtatatg gaaatgaatt taaaaagata
    58981 attttctttg ggatttcatt tttcattaga ttatctttca ttttaatcaa caaatctttc
    59041 ttcgtaattt tgtctatagt tattacaaat aatacaattt gatttcctct tatagtgtct
    59101 gagtacggca atacaattgc atcttcaatt tgaggaattt ttttaacttc attttcaatc
    59161 ttagtagaac taactttaaa tcctccaata tttataatgt catcacttct tccttcaaat
    59221 attaagtatc catcattatc ataatatcca atatcattga cagaataagg tttggttgca
    59281 ttatatactg catattctgt attcacaaaa atttttcctt ctctaatata tatatctatt
    59341 cctggaaatg gtctgcctac acttagaggt ttttcaatta gttcatcata gcataaatat
    59401 gtaatataat ttaattcact tgccccatag tataaaataa tttcagactt tggcatatat
    59461 ttttttagat ttcttgcagt atcttcaaat aataattgag accctgtaaa aatacttaca
    59521 actttgagta ctggttcttt taagtgttta accaaaagtt gaagttttgt aggtataagg
    59581 tatatgttag ttacattata ttgttttatt gtccttatcc atgatttaca attaagacct
    59641 gaacttatta caatactacc tccttcatat aatactgaca ttatagaatt tagattccct
    59701 gtaaaactaa gtgttccatt aacaaacaag attgattccc cagagatatt aaaaatacca
    59761 ttttgtatag gaaaaaatcc agcccaactc tcatatgttc tatataatac ctttggaaca
    59821 cttgtagaac ctgaagataa agctcccata caaatacttt tatctaaata atttataagc
    59881 ttcttatatt gatacatata tatattaaat ttaggattaa aaatagaaaa attaactttt
    59941 tttgctactt cattattgtt atcatcatat aaatcaataa cgtccataga ttcatcagat
    60001 ataatgattg aaatattatt ttttaataaa atatcatcta atactttttt agaaagatta
    60061 taatgacaaa taataggaat attttttgaa taattaatag caaaaaaact aatcaactga
    60121 aacattatat ttttagaata tattaaaatt gggatatttt cacctaaaga aatttgcttt
    60181 cctagttcct cactataact caatatattt tcataagtat atttttctcc atctataatt
    60241 aaaaaatttt tatttttatg cctctctttt tttattctta aaagttcata atagttcata
    60301 atctacaccc tttcaagcag taaagctgaa cctagaccac cagctgcagc tatagagcaa
    60361 attccatatc tacccttagt cttttctaat gcttttaaaa gatgtaatgc aataattgca
    60421 cctgaagcac catatggatg tccatacgct aaagctccac caaatatatt atatctatct
    60481 attaactctg gatactttct ttgaaacaac acatctataa ctgcaaatgc ttcattaaat
    60541 tcaacagcag atacatctaa ataattaagg ctttctatct ctaaaagttt atccattgct
    60601 tgtatcgctg atgttggact taaggttgca tctgttccta tcgtgcatgt gttaataatt
    60661 tttgctttag gcttcttttt tacatgttct aaatacttct tagaacatat tataataaat
    60721 gaagctccat cattaataag acatgaattt gctgcatttg taatagtttc ttttcctaaa
    60781 attgatggca tcctatctaa taacctttga ctcatcttat cacgtatacc ttcatcatag
    60841 gtactattat ttataggaga tataatatct tcaagtattt tttcctctct tgcttctttt
    60901 gctcttttat gactttcctt tacccaaaaa tccaagtctg ctttctcaat ttgatataac
    60961 tctgctactc tttccgcacc ttcgagcata ctattttggc tattatcatc aggagaaaat
    61021 tgagcaactg tataatttgg attatttgta ttatatcgct tatcattctt atgatatgtg
    61081 cgcattggtt gcagagaact actttcaaaa ccacctgcaa taattaaatc acactgacca
    61141 gatttaacct ttgaaaaagc tatgtctata ctcatcatag cagaagcaca ctgcatatcc
    61201 acagtaaaag caggtacttc atttgacact cctgctgtta aagtcattaa tctagctata
    61261 ttccctcctg tcccaactgc atttccacat ataatctcat caattttgtc aatttcatac
    61321 tttttaatta aatcttttaa aacacttgct cctagtaatt caggttgaac aaattgaaaa
    61381 attccatttt ttagacctat atgacttctc aatcctccta atataaaaac ttccttcata
    61441 cattacaccc ttccttatat tatagattta tcatagattt cttctgagta cattgttcag
    61501 tttagtgcct aaaaaagatg caataactga ctttattgta tctccaatta aaaatggtac
    61561 tactgctgca aaaagtgctg cttctactgt catttgatta aaatagcaca taaatactgt
    61621 tcccataaaa tatataatag gcattccaac aaaaattgtt attaatatat atctcttaat
    61681 atcattattt cgtcctttta ataaactaat aattagtgct gcaaacaaaa acccaaaata
    61741 aaaaccacct gttgggctta ataactttcc aataccagat gtacctccac caaatactgg
    61801 aatcccaatt aaacctatca ttatatatac accaactgta ctaaatgctt gtcttggtgt
    61861 taaaatcaaa gctattaaat ttatcattat tgtttgtgct gttactcctg ctggagtaaa
    61921 tggcaatggt atatatatgt aagatgatat acacaataat gctacacata ttgacatttt
    61981 agttaaatct tgaatattta ctttagtatt tttcattttt ataattccct tcatatgtct
    62041 tatttcattt agtttatatg tacatataca aagcttctat taaagctttg ttttagtata
    62101 aaataaagac gtttttttgt caactattat aattatttag gtttacattt aatttataag
    62161 taaactttat actgttactt tatattttaa tagtcattta taaaactttg cttaaaaaca
    62221 gctctattta gactatactt gtaaattata atatgatata tttttatata agattttatt
    62281 ttatataaaa aatagtaaca aatagtttat acacaagata tttaaaagga agtgatgata
    62341 tgaaagttaa tgaaaataat atagatgaag agttagatga acttagtagg aaagtttact
    62401 tagagtcacc agatttttgg ataagtattt ctgacaaaat aatagataaa gtatttgatg
    62461 aaatgttatt attttttgca gttaaatata actatttcaa tatagtatgc tttgctattg
    62521 aaaacaatta tctagattta aacatgcctt caaaaaacaa aaaatacact aatataatgg
    62581 agcatttgtt agatacatca aaacagaatg aagataaaaa aatttataat tatcttctaa
    62641 gcttaaataa aggtaataac ctagatgaaa gaccaatttt agatgaatta aactgtaata
    62701 ttagtagtga tacttttgaa aaaaaagata tatatcttcc aaagtactta tgtccaagtt
    62761 gtaattctaa tatatttgat acaggttata aagtatcaaa tgacataaca tataaatttt
    62821 cttctaaaga agctgaacct attgaaatgt gtagtgaagt ttcttctatc agatgttgta
    62881 attgtgatga attattagaa gatattacac ttgataaact atataaccta tgcaaaattc
    62941 aaaactgtaa taattgtggt tctaatttaa ctaaggtagg aattattgca aagaaaaaaa
    63001 tgaactttga tgagaatact gaaaaattta aagatgtctc aacattttac tgttgtggaa
    63061 attgtgactc tgaaataagc ttatctcaaa gagaacattt taagatttaa atatttatag
    63121 gctaaagtaa taagattaca ttattagata ctgtataagt tttttaaatc tgtaaaaata
    63181 aatacactta taagaattaa aattgctatg aaactagttc ttgatgaact aatttcatgg
    63241 caattttcta taatctaata attattaaat tttaacaaac ctttattagc attccataat
    63301 tatttaaaat tctttcaata ctctatagtt tataggtgaa agtatcttta atattatatc
    63361 aaacttatta tctttaaata gtaaatttgt taaatctgaa atacaccata caattttatt
    63421 ttcctctttt gtagctatat caattctgat taacttacat ctataataaa tatctttatc
    63481 ttcatatagt ctattttaaa aattactctt aaaaaatcaa ctagaaaata aattaattat
    63541 ccttttaata gaatgtaatc atattattta ctatattcca ttatgtactc tttaaatcct
    63601 gaatcttcat ttacttgttc ttttactata ttaaaacctt ttttattata aaacacaact
    63661 gcttttttat tatctttata tactgctaag cttagacttt tatatttttt agttgcataa
    63721 tctaaaagtt tacctccgat acctagattt tggtatttgg gctttataaa taaagctcct
    63781 ataaagtttt tatctattat gcttataaat ccttttattt catctccatc atcatataca
    63841 aatgtatctg atataggtat atattcgttt ttaacagtat tatagttatt ttgccagtat
    63901 tctttactta taaagtcatg tgctttgatt gtactttttt cccatatttc catgatttta
    63961 tttatgtctt tactgtttaa tttctttatc ataaattttg tttcacctca tttttgtatt
    64021 gattttataa tgtttgtagg taagtgtatt ttatttagat atgatttagt taatataagg
    64081 tggatgtttt agtatatgct attgttgatt ctacaatttt atatactgct ttattaatat
    64141 aaaataagtt tctaaatact cgttgatatt gctaatattc tacttatcta ccacttagtt
    64201 gatataaaaa ttgaaacttc actatctaat aatttaatta atgatatgct attacattta
    64261 tctatttcat aaccaccata aatcccaggt gttgaattta taaatatacc agcttgctct
    64321 aaatacatct tgtaactttt tatttgcctt tcacttactt ccaactcttc tgataattct
    64381 ttgcatttca ttttgcctct gctttgaatt aatatcatca ttctaagtgt attagctaat
    64441 ttactaatga gtaacccccc cctaataaca ttaattgata tttttattat ttatttctac
    64501 acatatgtac atataacctc tttatatttt atataagtag aattttttta taattttgac
    64561 atttaccata ttatatttta tatttgtaca aaattttaat agttaataca ataaaaaata
    64621 ttactgaaaa aataaacatc tataattcca gtaatattaa ttaattttaa acccttttta
    64681 ttatcattta ataatagtaa ctaaaaattg actaaattta accttatctc ttttaaataa
    64741 ttatttctta caataaaaaa ataaactatt tgaaccaaga acaagcttcc cattaccaaa
    64801 acagctgatg atgctacttc tatatcaaaa gaatttttaa gtgcagtaag tgcaaatatt
    64861 gaatgtataa cagccactac ataaggaaac aaaaatacta ttccaatctc aattgttgaa
    64921 gcttttttaa tctcattata tgtcataccc aatttattta actgttcata ctttttctta
    64981 tcaacttggc aatccatata aaatttatta tataaaaaac tacttgtagt aacaaagaaa
    65041 attaaaccta taaatattgc taaaaatata aatgtagaat acattaattt cccagttcct
    65101 atcattaatt ctttagagaa aaacaaatat ggttgcatcc ctgactcatt attaaattta
    65161 ttttcaaatt ctctgcatat ctctatagtt ttacttgaat cctctacttc aaatgcagta
    65221 aatctatcca ctataaattt tgattctata ctatcataaa aattatcctt taccacatac
    65281 atatttttgt aatatgctgg cataacacat tcttccactt gttttgccac ctttaaaccc
    65341 ctattcccta caataatatt ttctttgacg ttattatttc ctggtaaact tgatgataat
    65401 aatgatatac attcattatc ttcaaaactt atagacttaa cccctatagt ttctgtaatt
    65461 tctaagtact tagattcttt cataattctg acattaaaat cacttttctt gggaaataca
    65521 gttttaaatt caccattagt ccttgtatag tctacattct ctttttttag attattttct
    65581 ataaacatca ttctttcctt gtctttttct ttgctatcag gctttttagg gtcattatgc
    65641 aatgtcattg ttgaataata tattgtgtta ggatatatta aattaatttg acgctctaca
    65701 tctttccaaa aggaataaac tgtacctata gcagtaaatg ctactgcaga agttatagtt
    65761 atcaagaaaa atattctagc attatccttt accttataaa tcaagttcga aatccaaagc
    65821 atatttgtat tttttctata aaatctcttg tttagtttta gcttcttcat aaaaaataca
    65881 ctaaattgag aaaagaacaa gtaagttgct actatagtaa gggatgtaac tggaactaag
    65941 cgcagatatg ttgtatattg gtcggttgag gtaatagcat tataatacgc tattataaac
    66001 aaacaaatac ttaatatagc aagcattagt gatgttttag gctctggttt tggagttttt
    66061 gtacctttaa gtaacttcaa cacctgattt tctttaacta taaaagaagt aaatattgat
    66121 gttaatacag ctaaaatcat aaaatagatt aaagtagaca atatcgcatt aaatggtata
    66181 tagaatttta atggtgataa ttctaaaaaa gtagacataa tcactaaaaa tatctttgca
    66241 aagattaagc ctattattat tccaattaca gaagatacta tatttattat catattttct
    66301 ataaagatta atttcattac ttggcgcttt gatatacctg taatataaag tattccaaat
    66361 tctttatatc tacttttaag aaacacacta actgaataaa acacaaagaa gaataagaat
    66421 agatatgcaa ttatcttact catcttgaga ccttctttta aataatcagg aaattgagat
    66481 acatctaaat ctggatgact tgtaaacata ataaaagaaa atagcagtga agatgatata
    66541 acaatacttg ctaagtatcc taaatacgct ttaatatttc ttgaaatatt gttaatagca
    66601 aactgtttta tattcattag ttatcgcctc ccaataatgt aagtacatcc attatctctt
    66661 gataaaactg ctctctacta ctacccttat atatttcatt atatatagac ccatctttta
    66721 tgaataagat tctactacag tagctcgctg ctaatgggtc atgagttacc atcatagtag
    66781 taactttatt ttctttattt atcttttgaa atagctccat tacaaccttt gatgactttg
    66841 aatctaaatt acctgttggt tcatctgcta aaagtattga tggattgtta atcaatgctc
    66901 tagcaatagc agtcctctga gcttgaccac ctgatacttc aaaagttctt tttcctaata
    66961 aatcttttat acctaatatt gtagatactt cattcaatct ctcatcttgc tcttttacac
    67021 tgaccttatc taatgtcaat ggcaatacta tattttctcc tatagttaat gtatccaata
    67081 aattaaaatc ctgaaataca aaacctaact ctcttcttct aagtaatgca agttcctcac
    67141 ctcttaacaa taaaggattc ttgcccttta attccatagt tcctgtagtt ggcttgtcaa
    67201 tagtagaaat catatttaaa agagtagttt ttccactacc agatggaccc ataattccta
    67261 caaattctcc atcttctata ctaaaattta tgtcatttaa tgcgttgaaa attatttttt
    67321 taccatatac tttacttaaa ttatttacag ttaaaatttt cattatttaa ctatccttcc
    67381 taatttaatt tttgaaataa catatatata attgttaatt ataatttatc ttagtttaat
    67441 ttttatagat gataaatcta ttttttctat acactaatta tgttagtaaa agctaatact
    67501 gcaagtacta cacccattat tattctgtat acagcaaaca ctctcattgg ttttttcttt
    67561 aaaaaattca cgaatttttc cataactatt attgatacaa taaaagctac tataaaccca
    67621 actaccaaag caatccacaa tgttggtgtc attattgaat aatcaaattc aaataaatct
    67681 tttcctgaag aagccaccat agctggtact gctaaaaaga aagaaaactc agctgctgta
    67741 ggagaattaa gtcctgctat ccatccaccc ataatagtag atgcactcct tgacatacct
    67801 ggccatgcag acaaaacttg taatgtccca acctttaaag cttgtattgg agtaatttta
    67861 tctatattat ctgtggaatg attctttttt ctaaacaaag tttctactac taataataaa
    67921 atcccaccca ctataaatcc tataattaca gattgtaaat taaacaatga ttttatatta
    67981 tcgtaaaata gaactcctat aatcgcaaat ggtatacaac ctataattac attcattcca
    68041 aatctaaaac caatctttcc ttcttttcca ccagtaaata taaatttaaa aaattctata
    68101 acgctatctt ttatcttttt ccaataaagt actactactg ctaatatagc cccaagttgt
    68161 attacaacct caaacatttc agcaaattgc cccttaaaat ctattaaatt accaaccaaa
    68221 atcatatgac ctgtagatga aactggaata aattctgtaa gcccctctac tatagctatt
    68281 atgactgatt tcaatataaa aataacatct aaactcatca ataaacatcc ttcctttttt
    68341 acaaaatttc tttttttatg ttatttctct tatgttattt cttatattaa ttgtaattta
    68401 gattagaaaa tttaactatc tgcaaacatt acacaaacct tacatctatg taaggtttgc
    68461 aaataaacgc tcttaaattt tgattattta actatgaata tttcatttaa taaattgtat
    68521 aataaaaaag tacaattaac taaaaaccct attgtaacag ataatttaca tctttatcaa
    68581 actatttaca cctttattaa actatttaca tctttattaa agtagattga aacttctgta
    68641 ccttttccta cttctgattt tatacttact ttatgaccta aatcatcgca tacctttttt
    68701 actagataaa gacccatacc tgtagattct ccaaacttac gaccattttc tcctgtaaaa
    68761 aatgggtcaa ataccctttt taaatcttta gaaacaattc caattccttc atctattata
    68821 cttaaaatta catatttatt attttcttca gattttatag ttaggtagtt actataatct
    68881 ttagaatatt tcactccatt tgtaattaat tgacctatta taaatttcat ccattttaca
    68941 tctgtataaa cataagtaaa tttgtctact tgaatcttag gaatcattcc actttttata
    69001 aataaccttt tttcttcatt tacaatacta gatacaattt catttaattt ggctttttca
    69061 actataaagt ctttctgaaa attatctgcc cttgcaaaat gcatagccat atctaaccct
    69121 ttatttaatc tatctatttc ttttcttata ttttcagtta cttcttctcc ctcataatct
    69181 tgaagctgaa ggcttataac tgatattggt gttttcatct gatgaatcca ttggttgatg
    69241 aaagttaaat gttcactatg tatcttttta tgctcttgta tcattgattg atacaattta
    69301 tgttgttgtt ttaaaataac tgatatattt ctccccaaga atgagcttcc taaatacaaa
    69361 aatgactcct ccaaagaatg tactcctttt tcaaacagat tatatacttc tctattttta
    69421 taatacttat atattaaaaa acataacaat ataaaagaac taaataaaag aatatataaa
    69481 ctctctccaa aactaataaa cttcataaga ttacagtaaa taattgtcat aactatgcct
    69541 atataatata taaaaatata acctttataa tcttttaaaa ataacttcat actttctcct
    69601 taccaattct tatttaattt atatcctaac cctcggacag tctgaacagc attatctata
    69661 cctaattcct caaatctttt ccttatcctg ctaacattta catttaaagt attttcctct
    69721 acaaactcaa tatcatccca tatcttttct aaaagataat ctctacttac tacttttggg
    69781 tatctcttga taagacattc aatcagtata ccttcttttt tagttataat tagattctca
    69841 ccatttaatt ctagttctaa cctctctgga taaaacttta atccatctag ctccactatt
    69901 ctttcgtcta ttttaggtga atattcacca tatacccttc ttatatgact ctttattttt
    69961 gccattacta catcatagta aaaaggtttt gttatataat catctgctcc actttctaaa
    70021 gctctaattt ggtctacatt accatctcta gctgatataa aaattatagg taaattcgac
    70081 tgttgtctaa tacatctaca ccagtagaaa ccatcaaatt taggtaaatt tacatctaat
    70141 agaactaagt gtggcttaaa actttcaaat gtaatcatta tatcatcaaa gttttcttca
    70201 acaatcactt caaatccata tttctcaata taatctttaa gtaatgtact tattgatata
    70261 tcatcttcca caatatatac tttataactc atataattcc tcctaatata cctatttact
    70321 catcatatta acagtccaat tttaatatat tataatcttt ttacaatata taaatttaat
    70381 catattacta acttcacaat tactgacttc ataagtataa ccatattatt agacatctta
    70441 ataaaactct ataaaaaagt atctttttat gattaattaa tcattattat caactaatca
    70501 ttctaaaata aattgaaatt actaattcaa aatagaaaaa gttaccttaa taaggtaact
    70561 taaactttta ctatatttct tacttagtat tattttatca tagtttctac ttagcattat
    70621 agattaattc ttttatgttc acttttttct cttgtaactt attttttatt aagaagtctt
    70681 gtgtatcttc aagcgaagat atgtctttat ctgttatatc taaactaaaa tcataccaag
    70741 catataattc tttagtttct tcaagactta aatcaacttc tttagctact ttttccatag
    70801 cttcatcaaa attattattt acatattcta aagtttcctt ttcaaccttc ataaatcttt
    70861 caactatttc tggatgtttc tctgcaaagt ctgtgctaac agcagttact attataccat
    70921 ctactaaacc ttcaccattt gctacaagtt tactacctga cttcatagct ttaagagctg
    70981 ctggaccagc aatcaatgca gcatctacgc tgccatcaga aagagctgct gatgcctctg
    71041 gaatacccat attaacaaat tccacatcat ccatacttaa tccttcttta tcaagtgctg
    71101 aaatcaatac ctgatgtaat atagtccctt ttgggcctgc caccttctta cctactaaat
    71161 ctgctgctga ttttattgaa ttattattag tcaaaatcat aaatcctttt ggtgacctgc
    71221 tataagtgtt taatatcttt aactctactc cattagatgc agctattaaa gctgatgtac
    71281 cacctagtgc atgtaagaag tctatttctc cagcagctaa agcttgtgtt tgctctggac
    71341 ctgtagtaat ttcgtggaaa ttaactttta tattatcctt tttaaattcc tttccaaata
    71401 aatcatcctg tttttgaatt atagaaggaa catttaatgg tgattttaca taagttaaat
    71461 ttatttctga aagtttttct ccattatttt ttttagcaca tccagctaaa gatgctgttg
    71521 caagtgttaa tattgttata cctatcaata gcttttttaa tttcatttac tttactcctt
    71581 aaaatattat taatttcttt aaatgccttt atttgatttc attgtcatta aaatatcttt
    71641 ttttaattta atatagtaat ctgaagttaa atctctatta taaccatctt ctatattaaa
    71701 ttcttttact tttctttcgt ctgtgaaaac aactattttc ttgcctatct ttaacgcttc
    71761 atcaatatca tgagttacaa atatcacgcc tttattagtt gacttatgta ttcttataac
    71821 ttcattttgc atatcaatcc ttgtaaaata atctaatgcc gaaaaaggct cgtccataag
    71881 caacatatct ggattgaaag aaagagctct agctatgctt actctctgcg ccatacctcc
    71941 tgacaattca tttggataag agtttttaaa tttctgcaag ttcatcatct taagatattt
    72001 ttctacatca atattacttt tcctattata tttatttgat ttgttcaaat tatcatttgt
    72061 ttctaagctt ctaaaattat catttataat tttactgcta attcttttat tgtgtttaaa
    72121 ttttctaagc accttgttta aaatagtatt ttttctttga ttatgaaaag ctatattttc
    72181 acttacgttt aaccaaggca ttaatctgct ctcctgaaaa acaaaaccaa cattaggtct
    72241 atactcttta ttatcattaa taaacctaat ttcaccacta tttatacctt caagtgatgc
    72301 aattgctcta agcaaggtag tctttccaca accactttca cctaaaatta ctgttatatg
    72361 gtttctatct atgtttaaag agatatcact aagaactaca tgttcttcat tgtcaacttt
    72421 ataactttta tataaatttc taattatata tccagagtca catactaaat tattcatatg
    72481 cttccaccat ctttccctta cttaccttct tcacaataat cgaaaatatg taatctgtaa
    72541 ttattcctaa aagtccaatt acgattatac ctacaataac aacatctgtt ctagacattt
    72601 cttttccatc agatatcaag tatccaagac cagatgaagc tgctacaagt tctgccccaa
    72661 taatggctct aaagctatat ccaagagcta actttaaccc aacagcaata tcaagaatgg
    72721 catttggtat tattatttta aatattatct gaagctttga aaactcaaat actctaccca
    72781 cttcaatcag ttttgagtca caatttttaa tccctttcaa tgtgcttgta aagattggga
    72841 aaaaggatgc taaaactata attataattt ttgattcctc tcctatacca aaccataata
    72901 taagcattgg tattaaagca aggggaggtg tattccttaa aaaatttatt agagatttaa
    72961 agtattcgta tactccacta taaattccaa agaatatccc taaaggcaca cctatagcag
    73021 tacttattgc aaaacctatc aaaactcttt tcatacttgc atatacatta ataaatattg
    73081 aaccatcact tatcatattt aaaaatgtac tatatacctt ttcaggtgat ggtaatatat
    73141 aatcactcca aataccaaga taatttgtaa ttttccataa taaaagaatt ataaaaaata
    73201 tcaaaacact ctttactagc cttaaaaact tattattaaa tttattgacc atttacaaac
    73261 catctccatt attacaacca tctttacaat aaaacatttc tactatgtca tacttatctt
    73321 caaagccatt tcttattttt tgtatttcac ttaatatttc gtcttcactt gatacttcaa
    73381 gaactttttc aaaagtatct ttgaaatatc ctaatggaat tggtgattca tctatttttc
    73441 caagtgattt tacaccttct tgttcagcaa actctttcca tgtataaaac atagcattac
    73501 ccttatcctt aaacttttca cttgcaaagt ctcttaattg tgtacatgga caggttacta
    73561 ccaacatatc atctttactt tttatatatt tatcgtataa aaccctacaa gtccttacta
    73621 atattggttc tatcataggt acttcaaaat catttatcaa attatttctt tttaaaagac
    73681 ttatactttc tggacatcta caatccaaca ttgtattttc agtctttttt gtatactctt
    73741 tgtactgctt ttttacatat tctaactgtg gttcacattc tgctattatg tacccctttt
    73801 ttatcaaatt tgctttaata aggtgaaact tttctccata cattctttct gccactggat
    73861 ttattagaag atatttattc ataacgcctc ctattattta atattgaata ttattatcaa
    73921 ttaatagttt aaatgagcac ttaaattttg tcaatatatt aattttttct taataaaaca
    73981 atttttatct acatttttta atattttaaa tgaaatatta aatagtatat gtaatttatt
    74041 ttaattaata atgcatacat cttactaata atctatttat ataataatct ataaatatta
    74101 tataataata cttatataat tagtattatt atttaatagc aacagttatt cacactaata
    74161 aaagtagaaa gtttttaaaa tattatgaaa catcaaattt aattttaaaa ttgcatcaaa
    74221 aaatgaagtc tcaacagact tcacttttta taactcttat aaattattca aaatatttat
    74281 acttcccttt ctacttcaaa acgtaaaacc gtcttcaatg attcaggcga tacttttcta
    74341 gcatcactta tataaatttc acgatgtgtg taagattttc ttataagttt attttttttg
    74401 caaaattcgt tcataatgtc aaagctttct ggttcattat catatgaacc tatatgaagc
    74461 atttgaacac atagcccttc attaatagac ccaaatctaa ctttttcaag aagattatga
    74521 ggttttttct tctttactat atcagtagct ttatccaaca ggtcttgtgt aacaaaatct
    74581 ggctgacgta tcattatagt gtaaattaaa tcttctttaa taagtacatc taagccttta
    74641 cttttttctc ccttatccca tataccttct aaaggaaaaa ccgtatagtc aaaataccct
    74701 tctggtacaa ttcctttttt aggcatggtt tttattgcat atgccaaaga ataaagtact
    74761 cctattgctt cagtaaattc tttagaattt gggtctccac tcccttttaa catgataaat
    74821 ttctgttctg gtatttcaac tattacagga ttctttttag gtaaatataa ctctttatct
    74881 ctttttctcc attcatattt cataaaaata cctccatata tattttatac attgatatta
    74941 tcacgctaaa agtgacaaca gtgtgtcata attataaatt atattataaa atcttcatcc
    75001 tctaattcct catacactcc tttttggtat ccattaccct tagttgaaaa aaaatcatgt
    75061 gttttggttt cagtgctaag accatttaat acaattggat ttatagcatc tacagtatat
    75121 agcttctcaa accctagatt ttctaaagct ctattcgcat tgtattttaa aaagttcaca
    75181 acctcttttt caagcccact ttctctgtat ataacatttg tatactctat ctcattctcc
    75241 ataaggctat aaagtataga atacatcttc tcttctaaca tctccttatc cattttatca
    75301 aatgaatcgt atatttcttg tgctagcaat cctatatact taccatgtaa agactcatct
    75361 ctaagaataa gcgatattat ttctccacta gctaccatct ttccttgacc agatagatac
    75421 aatggataaa agaatcctga ataaaacaag aaactctcta aaaatacact tgtaaccatt
    75481 gataggtata atccctcttg atttgtagta ttttcgtatt gagctaaaac taaatcagcc
    75541 tttctttgta atgttggttc tgtttctatc cattcaaata attcatctat ttctaaatta
    75601 gaaagtagag tcataaatat agaagaataa ctttttgcat gtatttcttc catagtcccc
    75661 ataaaagaca agacagcttt tctttgtagg ttttctgtta aactcatcat agatggtatt
    75721 ccattattcc cctgttttgt atccagtaat gttaaaccac ccaatacttt tttatataat
    75781 tccttttctt tattacttag ttcattccat acctttatgt ctttagaaac agatatttct
    75841 tcaggaagcc aaaattgttt tacattttgt tcccaaaaag cttgtgtaaa tccatcttct
    75901 tctctattcc agttaactgc attatgtatt ttatgtaaat taaatgtcat atatttctct
    75961 ccctatactg aacacactag acactcatga gtatctcttg tcatctttgt tcttgtgtaa
    76021 tataaacttt tcaaaccttt tttataggca tatatgtaat gtctagctat atctctagtt
    76081 gtcttttcat ctgtaacaaa cagtgttgtt gatatacctt ggtcaacatg gttttgaact
    76141 gttgccacta aatctatgac ctttctcata tccatcctgt atgctgattg ataatacaac
    76201 atattatcat ttgttaaaaa tggcattgga tatattgtag tagagtctcc atatgttctt
    76261 acttctactg gctcagtaat tggcattata cttgatgtag cattttgaac ataactaatg
    76321 ctttgagttg gtgctactgc cattaaatat gcattatata tccctttttc ttttacctca
    76381 tccaataatt ttgtccagtc ttcttttgtt ggtatataaa tcccttcaaa taatgctctt
    76441 accttttctg acttaggtag ataagattgt tcatagtatt ttgataaaac cttgctattt
    76501 cttcccttaa catattctga tttatcaaat ccttcaaaag tttgatttct ttctatagct
    76561 attttcatag aagctttaat tgaatagtat ctaaccattg caaaaaatac attagaaaaa
    76621 tctatagcat catctgaagt atataaaata ttttctctta ctaagtatcc atgtaaattc
    76681 atagctccta atcctataga atgagacttg ctgttactat tgcttaccgt tggcactggt
    76741 tttatatctg tgctatctgc tacaaatgac agtgctctta ttgctgtttc aactgtactt
    76801 tctatagttt tattatccat tacattggct atgtttaaag aacctaggtt acaacttata
    76861 tcttgtcccc attcattttt tcctccatac ccctctattt ctgaaggtgt ttgatattga
    76921 aatatctcac aacataagtt agacatttta ataattccaa catcttttaa tgtatgttct
    76981 ctattagctg tatctatgta cactacatat ggatatccac tttcttgttg catctgtgct
    77041 atttttgtca acatctgcct aggatttatt tctttttttc ttatatcact atcattaacc
    77101 agtttatcat accacttatc catttctatc tcatcaagag aaactccata tttcttatac
    77161 acagtatgag gataaaatgc atatgctact tcattttttt ctgccaactc cataaattta
    77221 tctggtatta tagctcctaa tgataatgta gcaagcctta ttttttcatc tgcatttatc
    77281 tttttagtat ccatcaacaa ttcaaaatca ggatggaata cagtaaggta caccgcacca
    77341 gaaccttgtc tagctcccat ttgattaaag taactaaatg attgttctaa cattttagct
    77401 actccaacaa cacctccagc tgcaccttct atatctttga tagattcgcc agatgctcta
    77461 agtttagata aatttagtgc aactcctcca ccaatctttg ataagtgatt tgatgaagat
    77521 atagcatacg atataccttc tgtactatct tctacagata gtaaaaaaca tgataccatt
    77581 tctccagccc tttttcttcc agcatttaaa aaagtaggtg tagctggttg aaattcttgt
    77641 tttactattt tatctgccaa gtttaaagct aaatctttat cgccatttcc tagtgtcaaa
    77701 gctacaatta acactttatc attatatgtt tctaatattt cctttccatc attgctcttt
    77761 aatgcataat tttgataaaa cttatttgca ctcatatatg attgaaattc aaacttatgt
    77821 ttatcaatca tatcatatat atttttaata acatctatgc tatactgatt tattacttct
    77881 tttgaataat aaccttcatc tattaaatac ttcaatcttt cttctaggtt attaaatttt
    77941 ctcaactttg gctgtataaa ttcttctatg taactactta aggcttcctt atccttttcc
    78001 aattggtact tgccttgttc atttttaatt atcacttgat tgttaagctc tatccaattc
    78061 attatttacc gtccttttaa cataatcttg tgccataatt tcacttaatt tgcttattat
    78121 ttcttttggt acttatcagt tgaaatccca aaattaatct ataattatag aaatgcaata
    78181 agtatttttc aaaaacaaaa gcactacata tagtcagtgc tttatctatt atatactaca
    78241 tatagtatat acacaaattt ttttatatct aaacttttat tactttataa ttgacattat
    78301 ttttatttaa agaaattttt atcaactcaa atataaaaca atacaagctt attatgtcct
    78361 gtttaaaaat ttaaattatc tctaaatagc ttaaaaatat attctgatat attgaaaaaa
    78421 gtactttcaa gatgatattt tatatattat ttagaagttt tttatttatt gttatataat
    78481 taaatatatg actattttgc tactacatat ataaaaataa atttatgaag gtgattttaa
    78541 tgaaaactat ttctaaacaa gaattagaaa aaacacttga gaaacattat ttatggctta
    78601 aaaacaataa tgctggagaa aaggcagatt taaaagaatt aagattagaa aatatggact
    78661 taagaggata ctgcttaagt aatattgact tttcgtggtc tgaccttatc aatttaaatt
    78721 tagaaaaagc agatttagaa aactctattt ttaataattc ttatctttcg gattgttcat
    78781 taaaaaattc tatattaaaa aatgcagact taagtggtgc aagttttaga ttttgtgact
    78841 tatctaactc agatattaga ggagctaatt tagaaaattc caatttagaa tatgctactt
    78901 taaatggagt aatatcagat gaaagtactc gtttttttaa attgtattgt cctgaaacag
    78961 gagcatttat aggctataaa aaatgtttta attttagaat ggtacaatta ttaataccca
    79021 gcgacgcaaa aagagtgtct gctacatcaa atgcttgtag atgtgataag gcaaaagtac
    79081 tgtcaattac tagtgtagat ggtaaagaat cttttaaaag tgctcgtgca tatgctgatg
    79141 aaaattttat atatagagta ggagaaacga ttgtagctga agattttaat gaaaatagat
    79201 ggaaagaatc gactacaggg attcactttt ttttaactag agaggaagct attggttatc
    79261 tataaaatgc taaagtttta taaatatata aatacataaa caaaataaca aaagagtata
    79321 gattgtaatt tcaaaaaaat tataatctat actctttttt ctataacaca taagtacatt
    79381 ttatttcata aattataata aattatttat atctgcacta ttgattattt gagaaacttt
    79441 atctatatgt tctatcttac tatccaaact gtcataatac ttcttactct catctctatt
    79501 tctaaaagaa taaacttttt tatccaattt taaagcttct tttataatat ctatattcct
    79561 cttatttgtc tctcctacac taaatcctgt atctattata atcttggaat catttaaatt
    79621 tctaatagcc aaatcgaaac tctcatcact tataggctca aatggatttt ctgtaaacat
    79681 ctttatcccc atagttctac caatttcata atcaatatca ttttcatgta ttattccaga
    79741 atacagacct ataccttttt tagtaaaagc tctataaatt ggtgttgctt tacccccacc
    79801 accaattata aaaacttcat ttttagattt attactaatt tctactgaac ctaaaagact
    79861 attaaagttt ttatcatcca attcataaag cgaatttata atatcctcat ctactacatc
    79921 ttcaggctgg ccataagcta tgactttatt attttttata agagcaactt tatcacaact
    79981 ttttaatgct atgtctattt catgcaatga aagtattact gatattgact tttctttact
    80041 taattttttc agtatgttta tgagttccaa tctatgtttt atatccaagt gagttgtagg
    80101 ctcatctaat ataattattt ctggttcttg aacaagagcc cttgcaacca acaccttttg
    80161 tttttctcca tcacttagtt catcaaaata tcttttcttt aaatgtaagg catctacaga
    80221 ctccaatgct tcatctacca ttattaaatc tttcttagat agactcccaa aaaatcctgt
    80281 atatggatac cttcctatat tgactatatc ttgtacagtc attaaatcac ctaataatct
    80341 atttgtaagt actaaagcca ttttttttga taatgctttt cttgatatac aatttatgtt
    80401 tacaccattc aaatatattt caccttttat tggtgatata agtttagata aggaccttaa
    80461 tagggtagtt tttccagctc cattacttcc caaaagacat agtatttctc cattttttac
    80521 ttctacattt atattactta taacaacttt gttattgtat cctacactta aattattagt
    80581 tttcaacatg tctaagccct atccttcctt ttcaccaata acattactac aattggagct
    80641 cctaataaag atgttattgt acttattggt aattctactg gtgataaaat tactcttgat
    80701 attaaatcac aaagagctgt aactatactg cctaaaaata ttactactgg tatcaaaact
    80761 ctattatctg atgttttaaa tatcattttt gaaatttgtg gcactgcaag tccaataaac
    80821 cctataggtc ctgcaaatgc tgatactatt gctgctaata tactagatat aaatactatc
    80881 aaaactctaa aaaatcttat atttatgcca atactttttg catactcttc tcctagtaaa
    80941 aaagcattta aaggttttat tatgaataca gtcatcaaaa gacctaatat accaactatt
    81001 ataagtattt ctaccttttc ccatgtaaat ccagaaaaac ttcccataga ccacatagta
    81061 aattctttta atttatcact atttgcaaat gattgtaaca catttgttat accactacat
    81121 acatatccgg tcataattcc tattattaaa agagttgtta tattctttac cttatttgca
    81181 aataaaatga ctatagacat tacaattaaa gctcctataa aagctgctac aaatatactc
    81241 attgaattta cactctcaaa tcccaatgtg tatcctccca aaatcatgag agctactgat
    81301 aaagtagaac ctgaagatac tcctaatata tacggttcaa ctattgggtt tttaaaaaat
    81361 atctgtaata aaatacctga tactgctagg catgctccac ctaccatggt tgcaagtact
    81421 ctagggattc ttatattgta tattatttta taatgttctg tattatccat acctcttaat
    81481 acaacatcta ttacttcttt aaattcaatt tttacagagc caaatgctac acacactaaa
    81541 aaagtaagta tcatacttat aattaatact ataaatattg tcgtattcct tattctcatt
    81601 ttattgataa aatccatttg tatactcctt ttagcaaagc tatattaact aaaatctata
    81661 aaacctatct ttttgtgtcc tctctgtact atttatcata atttatataa tgtttgattt
    81721 tttcacctgt agtacctggg tggaacatct caactaaatc tactattaat tcgtctgtct
    81781 tatctactga ttgataataa tctggagcaa acacccatag gttcccgttt tttacaacat
    81841 ccaaatcagc caatacaggt gcactagatt ttatagaatc taaatttggt gcataatctg
    81901 aacttgatga gtatacaaat atatctgcat cttttgcaac ttcatgcaat tgttcaatac
    81961 ttactcgccc ttcatttgaa tctattgaag cacatgcatt tattccacca gccattttta
    82021 tcatattatc cacatatgaa tctttaggag ctatataagc acttccttca tatacagatg
    82081 cccatacaac tttaggttta gctttgtttt ttattttttt acttacttct tctacctttt
    82141 gtacagtttt atttagttca ctagtagcca tatcttcttt atcataaaat gcagctatta
    82201 aacttgtcca ctccattctt ccaaatggat tttgctcttt atactcattg tctacaacat
    82261 aattaagccc taattctttt aatttgtcaa taaattttgt atcagcctct gataaaccac
    82321 tactcactat agttaagtca ggatttaagt ctactatttt ttcataatct ggtgctaaat
    82381 ttgaaccaac gtaatgtatt tttccatctt tcattaaatc tttaatttct ttaatagtcc
    82441 agtactgctc ttgtgttgtt acggctttta tactatctag ttcatctatt gttcttagag
    82501 aacaagcctg tgttgtagat gctattaata cattgtccac aggcgttcta actatattag
    82561 catttttata tttttcagga attttaatct tctttgggat taaaatcagt tctcgattat
    82621 ctccatcagt tactttttta attccatctt ctaaatcttc aacactaaat ccactagcat
    82681 attttaattc taatttctta ccagtactag cttgtttatt ctctttgtca cttttactac
    82741 accctataag acctcctacc ataaaaatta ccatagcaat taaaaaaaac tgctttaaaa
    82801 catttgattt aaatttcatc tctttcactc caatatctta ttttttatga gtggctaaaa
    82861 aactttaata atatatgctt tttaaagtat acttattaaa gttatcctaa agtcatcttt
    82921 aataagtata accttttaat aaaataaaaa acttctccta atttaataag gagaagttca
    82981 tatatcaact ttacaaaaac gaattgatac ttcattttat tataaattac aatctccacc
    83041 ctaaattgaa cttatataat actgggcagg tctcctgact cacggctttt cctccatcac
    83101 ccttcccaaa atttttataa atttcagtgg attttgattt cgtatccgta tacagtagcg
    83161 ggggctgtag tgaattttca tcactttccc tattaaacta atatagtacc ctagtattat
    83221 tttaatttaa tttttattaa gataattgta atttattgtt gacctctttg caaggttgat
    83281 ttaacatata tttaattcat tcatcaattt tttaatttca catacttgtt tttcatttgg
    83341 ttttacaaat ggagcagtca tataatcact tatttgtaac cctctgatat tcatagcttt
    83401 tttcattgct ggtataaatg gattggttat taagtaaaaa tccattgctt tattaatata
    83461 tttttgaatt tctataacct tctccatatc tctattattg taagcttcag cccatttgga
    83521 gcaaacttct ggaattaaat ttgataaccc ccctatacaa cctgctcccc cagacattag
    83581 attatgtata aagttttcat cataaccact ataaacctta aattctggga actctatttt
    83641 catagtattt attaaatctc tggtatgtcc catcaaagtt actgtatcct tatatccaac
    83701 tatgttttta tgttttcttg caagtcttaa tgtaagttct ggacttaaat cataaccagt
    83761 tctatctgga taattgtaca aatatatttc agcctctgta ttcttagcta tttcactata
    83821 aaataactca atactatcat catcaagaga gaaataataa gggcttatta tcataacacc
    83881 atctgctcca ctattatgtg catagtttga aagttctata gattcattta tacacatacg
    83941 acttgtacct ataaacactt ttactctctt atttacatga ctagttacta aatctataag
    84001 ttctttttgc atatccatag acatattaaa gaactctcca gtacttccca ttatgactag
    84061 tccatctaca cctccatcta taagatggtc atacactttt ttattacctt ctttatctaa
    84121 atttccttct tcatcaaata ttgtaacaac tggtgtcaaa aattctgctt tcataattta
    84181 aactcctaaa atttatttta atatgttcat taatacatca gatttagctt taatattaat
    84241 tatattaaaa cagttactta atgtaatttt ttaaaattcc aatactctca atctcacatt
    84301 caactacatc tccactactc atatatcgag gaggtgaaaa tcccatccct actccagaag
    84361 gtgttccagt agctataatg tctccagcct ctaaagtcat accttttgaa agttctgaaa
    84421 ctatgtctga aacgtctgat atgaataacc ctgtattaga actttgtctt tcttcaccat
    84481 ttaccctact acatacttta agttttatgg gaaaaggaac tgcactttta tgtaaaatac
    84541 atggacctaa agaggtaaat gtatctaatc cttttcctct aaaccattga acatgttctt
    84601 tttgcaactt tctagcagaa atatcattta taacagtgta accaaagata taatcttcta
    84661 cctcttcttt tctaatatta acacctgttt tacctattac tactccaagt tcaacttcat
    84721 agtctagttg attatttatc tcaaaatgac catctatctc atcttctgga cctattgcct
    84781 ttgtcacacg ttttgtaaaa taaaccgttt tttgtggttc ttcaaaacta tcatcaaagt
    84841 gagtttgagt ttcttcaaga tggtctttat agttcactcc cacacataag atatcatgta
    84901 ttggtttttc tataggaaca tgtacagtac atctctctct tttaaaaaca tcataattat
    84961 agaatgtact ttcatttata atagttttca acttaataat atcttcacta gatacatttt
    85021 gaattaattc atttaaatcc ttaaaatttc tactgagatt taaagagtta acatctataa
    85081 taaataattc atctttagaa aaaactccag ttcttttaca ttcattttct ccttcagtaa
    85141 aacttacaaa cttcataatc tcacccttta ttaaaattct atgtatttat tttcttactt
    85201 aaagattata catagttgca tactctagct attgccattt cagtatatcc aagggactct
    85261 gcttttgatt gttttccatt tgctacatct acaaccatat ttaacaaatc atctgttaat
    85321 tcttccattg tttgtggacc atatataact ggactagcat caaaatccag attatcttcc
    85381 atatttgcaa aagttatttt atttccagtt atttttatta ctggtgctat tggatttcct
    85441 gatggtgttc ctcttcctgt actaaatacc actatttgtg ctcctcctgc taccatacca
    85501 gcaacagatg aagcatcatt tccaggggta tccattataa caagaccttt cttatcaatt
    85561 tgtttggcat aatcatatac ttcacttatt tgtctatgac cacctttatg tatacaacca
    85621 agtgattttt cttctaaact tgttaaacca ccagctatat ttccaggaga tggattacct
    85681 tctctaactt cttctccaac caattttaat gaattttcat atctatgtac tatatgtaat
    85741 attttttctt ttacttcttc atttactgct ctcctagcta gtatatgctc tgcacctata
    85801 aattcagtag tttcacttaa aattgatgtt gctcctaaat cgacaagttt atcacttaat
    85861 tcacctatta atggatttgc tgctaatcca cttgttgggt cagacccacc acattcagtt
    85921 cctagtataa gttcagatat tggaaattct tcttttctaa gcaaagaagc ttcttgtgcc
    85981 atttctctag cataacgaac agctttttct atagttttta aagttccatg ttcttcttgt
    86041 attacaagag tttttattgg cttatttgtt ctctctttta ttgcatcaac taccaaatca
    86101 ttttggcaat tttcgcatcc caaagataca actattacac catatacatt tggatttgct
    86161 gcataaccag ccataacatc catagttaat tgttggtctg aatgtacttg tgaacatcca
    86221 ttctgattat taaaggtaac tgccccttga acttgtgatg ctattattct agttgtatct
    86281 gatgcacaca cactcgtagg caatattaat atgtggtttc ttataccaac tcttccatct
    86341 ggtcttttat atccataaaa tttcatactg atttcctcct aaaatagtct atatactttc
    86401 tataattttt atatattata atttttatta cactcactaa tcaaacagct aaaaacctat
    86461 ttaaaaacta tttgacacta aaatcaatga atacaacttt tataactcta taaatattat
    86521 tttatatatt agtcataact tgtttatcca aatcctctct aacacttcta acattatgaa
    86581 tatgtacatg ttgaccactt tttatctcct gatttgcttc tcctatatgt tcaccatatt
    86641 tagtaacttt atcaccttgg gccatatctt ttatagccaa tttatgataa atagttatat
    86701 catctagtgc tgtaatcgtt tttactgttt tgtcttttaa tttaaaacta atttcactac
    86761 ctttagctat tgcttcaata gctactgcaa cattatcttt ttcatcaatt atcactgcat
    86821 ttatcattat tcttgctctc ctttataaat aatataattt tattaatatc ctaaaatcta
    86881 attaaagaat ttattatttt accaacaact cactgtaata taaattaagc tgttattgta
    86941 ccatcctcat tttgttttgg gaacttagga catccatact tcttagccca ccaactagtt
    87001 ataagaggta ctaatattgc agtgacaact actgcggctg ccacttgtgt tgtagctgtt
    87061 gcaacatatg gttgccatga tgggtcaact aatgcaacag cagcaggtac agcaattgca
    87121 tttccagcag ttgtagctac agcccatcct gcatatcctg gacgttttcc tataaattta
    87181 tcacagaata ctataaatat acctcctaca aatacagata ttaatccaag taatactcct
    87241 gataaacctc cttttagtat acttgcaaga tttatgctac taccaagtgc aaaccctaca
    87301 aaaggtacga gtaaattaca ccctggctct aaaaactctt tcattttttt gtcaaaattt
    87361 cctattatca taccaacaag tattggcact attgcagcca ataatgacaa caatggtaca
    87421 tttgcaagac ctgaagtccc aagagctatt aatgtaaaaa atggtccatc atttaaagta
    87481 agtaaactca tagttgcaca atctgtttca tctccatatg aagtcatcaa tgaaagatat
    87541 atactaccat tactatttgt aactgcactt ataacagcca atgttgtaag accaaggaca
    87601 ccatccattc caaatagctt tcctataagt atacctatca aagcacctat agcaaattta
    87661 gaaccaagaa gtataccacc tctctttaaa acttttggca tttctctaaa ttgaagctga
    87721 gttcccaagc ataccagttg aaccccaatt atagttgcag acccaacact tgaaaatact
    87781 gctgtcgtaa acgaacctat ttgtacaata tctgggcaaa aggtattaat gaaagctgct
    87841 ataaacattg gcactatcat catacctgca ggaatttttt taataaaatc cattatcata
    87901 aagttaatcc tcctaaaaca tgatatttaa ttactcttat taagttttta gctttaattc
    87961 taacggccgt ttcggttcgt atttaagaac taagttttta attacgaatc tttgaattaa
    88021 tgatagcatt ttcctattta tgagtcaata aaaaaaataa atttttgtca aaattttttc
    88081 aaactttctg ataatacctt gtatattatc aaatgacttc cttatatgca aaaatacagc
    88141 gtatttaaaa ttatatagta ttcaaaacta taaagttttt atacagtgta aaaaatagat
    88201 aaaatataag ttcataatat tttgatattt acatataaaa ataactattg catatattta
    88261 cttaaattat ataactaaat atcacttact ttctttaggt acattttgga aattagttat
    88321 tccttaatgt acagtctaaa aaaatatcta tctgtatcta aatcaagagc ttatttatat
    88381 ataaatttac atatttattt tctacattgt aaaatatcgt atagtgtgct aaaatcaaca
    88441 tattctaata ctttaaatta ataaaaagag ggggaaattt atgacttcag ataatcatcg
    88501 cccaacagct aggatattag atattttaga agcattagca gaatccgaaa atggatatac
    88561 tcttacagaa atagcagatg ttatcaatgc tccaaaaagt agcatctttc ctatagttca
    88621 tacacttaat gctagaaaat acattactat tgataaaaac acatccaaat attctatagg
    88681 gatacgttct tacacattag ggtcttcatt ttttgaaaaa agaactattt ttaactatat
    88741 aattgacgaa atgactaata tagttaattc ctgttctgaa acttgtcagc ttggtatact
    88801 tgacaataat gaagttcttt atattggaaa agtagattct ccagaaccaa ttagacttat
    88861 ttcttatgtt ggaaaaaaaa tacctgctaa ttgtacaggt cttggaaaag ctcttttatg
    88921 tgattttgac aaggaaaaac taataagctt atatccagat ggtctaaaag gttttacaga
    88981 aaaatcaatt acagattttg atgttttata cgaccagctt cttgaagttc aaagaacaca
    89041 aatagcttca gagtgtgagg aatcaatgga acacctaaaa tgtcttgccg taccaataag
    89101 aaaaaatgga tgtataagag ctgctgttag tgtaacactg cctttattta gagctaatga
    89161 cgaaaaaata gaactgataa aaaaattact cttaaatgct cagaaaaata ttcaatcaat
    89221 gatggaagaa cgtaatgaag acattgtaat aaattagatt aatattctgt atatggaaga
    89281 tatcaaaaca taataattaa aaattttgtt ttaaactaat gtatttattt gatttatact
    89341 ataaaaacaa agttgctgat ttaactacag taactttgtt ttttttataa tattttttat
    89401 atttttattg acatgataaa aatactatgt taaattctaa tcataatcgt aattacgaat
    89461 taagttctta tataagaacc aacaatccta tctcaatcta agatacgata tattatgagt
    89521 aagttagtga aaattgttag atatatattt ttacataaaa atcaatgaaa ataaaaggag
    89581 attttagtta tgagcattta ttatgtacca ccaataaatt tacttgggaa aggatgtctt
    89641 aatgaagcta aagattcaat taaatcatta ggaactaaaa aagcatttat tgttagtgat
    89701 aaatttctag tttcaaatgg tactgtaaaa aaagtaacag atattttatc tcagatagat
    89761 gtagactttg ttgtttatga tgaagttaaa caaaatccaa ctgtaactaa tgtgaataaa
    89821 ggacttaaaa tattaaaatc agaaaattgc gacttcgtta taactatagg aggtggttct
    89881 ccacaagact gtggtaaagc tatctctata ttagccacta atggaggaga tattagagac
    89941 tatgaaggta tcaacaaaac ttctaaaaaa tgtcttccta tcatagctat aactactact
    90001 gctggtacat ctgctgaagt tacaataaac tatgttataa ctgatgaaga cagacatgta
    90061 aagatgataa tggttgatac caattcacta gccacaatga cagtaaatga cccagaatta
    90121 atgattagca aaccatctaa tttaacagca gcaactggta tggatgcact aactcatgct
    90181 atagaagctg tagtagctaa tggtgcttat gatgttacag attcaactgc cttatatgct
    90241 ataaagcaga tatttaaata ccttccaaga gctgttaaaa atggtaatga catagaagct
    90301 agagaacaaa tgtgttatgc ttgtttttta aatggaattg cctttagtaa tgcaggtctt
    90361 ggaaatgtac atgctatggc tcatcaatta ggaggtttat atgatttacc tcatggagta
    90421 tgtaatgcca tgctacttcc aatagttgag gaggaaaatg ctaaatcagc ccctgccaaa
    90481 ttccgaccaa tagctgaagt tattggaatg gatgtaaata ataaatcaga taaagagtgt
    90541 gttgactttg taatagataa gataaaagct ctttctgaag aggttggaat cccaaaatct
    90601 ttaaaagatg taggtgttga taatcctaat tttgaattgc ttgcagaaaa ctctatgaaa
    90661 gatgcttgtg ctggtgctaa tcctgttttc tttgataaag ataagattat agaattgttt
    90721 atgaaaatat cttaattatg tatatttata agagagtgcc tcaaaatatc tttgttttgt
    90781 taatagcact ctcttattac actttttaaa tagtactctt tatgagaagt taatatataa
    90841 ttctattaat ttaagaatta ttatcccaat aaaaaactca aaatttttct taatgaatta
    90901 taactgttgt tgtatctggt atattatcat atatccactt tgcattttcc gtacttaatc
    90961 ttatacatcc atgactaaga gcttcaccaa gtctgccatc tattatataa cttcctgttg
    91021 aatcatatag tacagaatga tagtaatatc cacctttaat tctagtagca tacttaactg
    91081 aatactcatc cgttccaaaa gaaggttttc ttccacttat ataaaatata cctgttatag
    91141 ttggagtctc aggcttgcct atagtacatt gccacttgta aagttgtccc catcctccat
    91201 tatctttctt aaaaatataa gtatatttat ttctaaggtc tgttacaatt agataatcgc
    91261 tagtactttt aatgttattt tcgttaacaa atttaatcat gtcagtataa aaatacttat
    91321 aatctaattc atttggttta ttcccatcaa ttgataaaaa ataattaaaa acatatccta
    91381 ttttatcatc atatacaacc ttactccaat ctccatctac ttgaaggact tcaaccctag
    91441 atttttcagg aattactgta attatattgg aagttgtaga tttatcttct cttaagttta
    91501 agttactcca agcatattcg cttactgata ccaaatcctt atacacatat ccttcttgag
    91561 aattgtacat caccttaatc cagtcatctt cttcatccaa tacttccatt ttggctccct
    91621 gaggtattac tgttatgata cttgaattag tagatttagc agacctcaaa tttacatttg
    91681 ctaaagcata tttatatata ggtgctctat attttttaat atttagttta gttaatttca
    91741 cacatatcca tcctctcata tgtcttttta caacatttta tgaaattcga tatgtattcg
    91801 ttctcctatt ttcaaacttt acttgtatta ctaagaatat tgctacatat acttatatat
    91861 tctcacctat atttatctat ttatttaaat ttatgatatt acattcttta atatactctt
    91921 caattatacc tctagccaca ttaaaataac ttatacactt gtcaactatg actgcatcac
    91981 tactaactac tctatctaat ttttccaata caacatctgc attttttact aattctactt
    92041 cagtttcaat tccccaagtt ttggcatgct ctaatatcct atactttaaa tttcctgaat
    92101 tagaaactgg agcatctaaa taaaatttaa gattctgtgc attgtattta ttaaaaaatt
    92161 tacctatcag attcaaagct tcttctgttt tatctataat cttataggtt cccctaagac
    92221 ctgctaaatc tcttatattg ccatcacttc ctattactag agtaccacca gataatgcta
    92281 cctctatgga tattatcaaa ttaaagccat ctatatttac ggtatcttct ttgattttat
    92341 ctagagaaag cctttttgat tgtcttataa tatttttttc atcagtacag actgcccttt
    92401 ttaatgcatc tctttgtctt atagaaaact gatatctatc accaacaaaa gtaacgactg
    92461 ggtctatttt gtaatcccta tttaataacc attctatttc ttctttagac tttataagtc
    92521 ttgttaattc tttacttgaa aaccatcttt tatcactatc atcaaaccct cttttagata
    92581 tcttatttat atcatttcac caccttgctt taaagatata actgtactac attaaataca
    92641 gattatttca ccttttaaga taatttttaa taccattatc ttatccattt agattattca
    92701 atatttataa ttaattataa cgcttaaccc cacttaataa tacagaataa tagattctat
    92761 attatattta taagtttata acataaatag gtagtgtttt aaaataaact ttttagttca
    92821 taagataatc ttcttttata aaatcaaata tatttttatc atttaaacag taaaaaagag
    92881 acttatctct attttttgag atagcctctt tctaaaatct acttataatg ataattttag
    92941 gtttatatat taaacttatg ttatacttct tttatcatat taggagatag aatatcatta
    93001 agagcttctg tcatagttgg atgtgcataa acacggtctc ttaaatatgt atactctact
    93061 tctaaatcta cagctaattg aataagatgg ataatctcac ttgaattttc acatatcata
    93121 ctagctccaa gtatcttatt ggatttttta tctatcacta tttttataaa cccatctgcc
    93181 ttgccaatct gtttagctct tggaattgct tctactggca tctttgccac taatacttca
    93241 tatccctttt ctttagcttg tttaacatta agaccaactc ttgaaaatgc tggacttata
    93301 aatatagaat ttgggatatt ctttctatca ttagttgttc tagtcttatc tccaaacaac
    93361 tgattaataa ctatgcgata atcatccaat gagatataag taaattgtgg acctccattt
    93421 atatctccta tagcccatat atgctcttta ttagtcttaa gagtttctga gactttaata
    93481 aatcctcttt catttaattc tatacctgca ttttctagcc ctagctcctc agtatttgct
    93541 tttcttcctg tagctacaag tatcatatta cttgttaatt ctttagattt accatctact
    93601 tcatattcta ctattgctaa ttcagaaact tctttaatct cttttatatt tgcattatta
    93661 acaattttta catttctctt tgcaagtaat tttattattt cttctgaatc ctcaacatct
    93721 tcatttggta aaatgccatt attagagttt aatattgtaa cttctgctcc aaatgaacta
    93781 taaataccag caaattctaa acctataaat ccagctccta taattgtcat ttttttaggt
    93841 aaagttctta gattcataag agactcacta tcatatacta tattcttatt ttcaattccc
    93901 ttaatattag gtataaatgg tcttgaacca gtattaataa atatattatc tgctactaac
    93961 tcataaattt cattttcagt tttgacttga actgtttttt catcaatgaa agttcccatt
    94021 cctgtgaata tagttacatt ctcatttgaa tttaatttat tatagttagc ttctctgagt
    94081 tttgtaatta aagtttcttt tttatctata gctttctcat attcagcttg tacttcatcc
    94141 caagaattta tattctttgt ttttacgctg tttgctgaat tttctaagct ttttgttggt
    94201 atacatgcca catttacaca tgttccacca tacatcttgt ttgacttttc tattaaagct
    94261 acttttaatc ccctgtttgc caaatcccct gctaatgttt ttccaccttt tccaaatcct
    94321 attattatag catcaaatgt ttttttcata taaaattcaa ccctttcttg tattgtttat
    94381 acttttacta taacaagatt atttaggtgg tacaattagg cacttttttg taaccttaaa
    94441 ttgatttata aacttatact taaatacttt tatttatttc tctgattttc aataaatgat
    94501 gccatagttg ccttatccat agctcctgga atatactgag ttacatatcc ttccttgtca
    94561 attataaatg tagttggaaa agcattaatt ccatattgat atgctaatgc tccatcttca
    94621 tcaagtacaa cagggaatgt ataaccttgg tcttttaaga agttaactac atgttccctt
    94681 gaaccttctc tacctaaatt aggtgaagct actcctaaaa ttactacatc atcattattt
    94741 ttattatagt ctttatacag ttgttctata taaggcattt cttctttaca tggaggacac
    94801 catgtagccc aaaaatttaa gaatacaact ttgccttcat aatcacttaa tttatgtgtt
    94861 ttaccatact ggtctgttaa agtaaaatca atagacttta ttctatcttc atcattagac
    94921 cctttatcat tattgtcatt attagagtct ttttgactat cattaccatc agaattttct
    94981 tctttatcag tagaattttc tcgcttattt tcttcttgct tgctttcaat tttactattt
    95041 tgtgacgtat taaagtgttt gcttatactt ccaaatccat ttaccatcat taatattcct
    95101 gatactataa gtataatccc acctattttt ttaataattt ccatgttaga ttttatctta
    95161 tctatagttt taaatagttt actgtaaaac atagctgtaa ttatgaatgg caaaataaaa
    95221 ccaatagtat atactgctat taaaagattc gcactaagat gattacttga acttgaaacc
    95281 atgactaata cagatgccaa tattggacca atacaaggtg tccatccaaa actaaatgta
    95341 aaacctaata taaaagctgt tattgctttc atttctttaa acttaacatt aaatcttttt
    95401 tctctattca atattgaaga tttaattatt cccatgtaaa ataatcccat gataataatt
    95461 ataatcccac caataaacat tattaaatct ttattttcat taaaaaacat actcaatact
    95521 tttactgatg aacccaatat aaagaatgtt gttgagattc caagagcaaa aaatatagta
    95581 tttttaaata aagaacttcc tataaaactc gtctttcctt cttttaaatt ttctacacta
    95641 ctatttgaca atatactcaa ataaataggc aaaattggca gtatacatgg tgaaaagaat
    95701 gatactatac cttctataaa tactaaaaat aaatttacat tttgcaaatt aaaattcctc
    95761 cttattttat ttccaatatt acaatcccat ttctaaatta aaatctaact aaaaaattat
    95821 gtaattttaa tacatttttc tactcatagc ttaattaaat tttcattact tacagagtaa
    95881 attttagtat ttatcacaat ttaaattttt attgctccac ataatcattt tttccatgac
    95941 aggaagcaat cgtaatcctg attcacttaa aaaatatgtt gactctacaa cattattaac
    96001 caactttttt tggttgacta taatcccctg ttcaattaaa aaatctagtt gttgaactaa
    96061 cattttctta ctacagtctg gtatagctct ttgtagctct ccaaatcgtt ttttatcttc
    96121 ttgtattttc catataatta tagccaccca ttttttacct aaaatatttg ataaagcttc
    96181 tactggacaa ttatatatca tatcatctat gttgtacatt ttattctcct cttttcttta
    96241 tttaatttat taaacaaact ttt
//
LOCUS       DAMJYX010000004        86535 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJYX010000004 DAMJYX010000000
VERSION     DAMJYX010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 86535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.004"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            153..491
                     /locus_tag="PC479_000273"
     CDS             153..491
                     /locus_tag="PC479_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2802863.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(618..1907)
                     /locus_tag="PC479_000274"
     CDS             complement(618..1907)
                     /locus_tag="PC479_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2802864.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1987..2691
                     /locus_tag="PC479_000275"
     CDS             1987..2691
                     /locus_tag="PC479_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2802865.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2783..3829
                     /gene="selD"
                     /locus_tag="PC479_000276"
     CDS             2783..3829
                     /gene="selD"
                     /locus_tag="PC479_000276"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2831..2833,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2802866.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3847..5259
                     /locus_tag="PC479_000277"
     CDS             3847..5259
                     /locus_tag="PC479_000277"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2802867.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5373..7256
                     /gene="selB"
                     /locus_tag="PC479_000278"
     CDS             5373..7256
                     /gene="selB"
                     /locus_tag="PC479_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2802868.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7361..10099
                     /locus_tag="PC479_000279"
     CDS             7361..10099
                     /locus_tag="PC479_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2802869.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10218..11162)
                     /gene="manA"
                     /locus_tag="PC479_000280"
     CDS             complement(10218..11162)
                     /gene="manA"
                     /locus_tag="PC479_000280"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2802870.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11335..12696
                     /locus_tag="PC479_000281"
     CDS             11335..12696
                     /locus_tag="PC479_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2802871.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12784..14673
                     /locus_tag="PC479_000282"
     CDS             12784..14673
                     /locus_tag="PC479_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2802872.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14688..15143
                     /locus_tag="PC479_000283"
     CDS             14688..15143
                     /locus_tag="PC479_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2802873.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15202..15519
                     /locus_tag="PC479_000284"
     CDS             15202..15519
                     /locus_tag="PC479_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2802874.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15544..16806
                     /locus_tag="PC479_000285"
     CDS             15544..16806
                     /locus_tag="PC479_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2802875.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16911..17975
                     /locus_tag="PC479_000286"
     CDS             16911..17975
                     /locus_tag="PC479_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2802876.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            18029..19207
                     /locus_tag="PC479_000287"
     CDS             18029..19207
                     /locus_tag="PC479_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2802877.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19398..20456)
                     /locus_tag="PC479_000288"
     CDS             complement(19398..20456)
                     /locus_tag="PC479_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2802878.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20523..20894
                     /locus_tag="PC479_000289"
     CDS             20523..20894
                     /locus_tag="PC479_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2802879.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20931..21605
                     /locus_tag="PC479_000290"
     CDS             20931..21605
                     /locus_tag="PC479_000290"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2802880.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21854..22693
                     /locus_tag="PC479_000291"
     CDS             21854..22693
                     /locus_tag="PC479_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2802881.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22937..23033
                     /locus_tag="PC479_000292"
     tRNA            22937..23033
                     /locus_tag="PC479_000292"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22971..22973,aa:Sec,seq:tca)
     gene            23183..23890
                     /locus_tag="PC479_000293"
     CDS             23183..23890
                     /locus_tag="PC479_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2802882.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(24032..24868)
                     /locus_tag="PC479_000294"
     CDS             complement(24032..24868)
                     /locus_tag="PC479_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2802883.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            25056..25961
                     /locus_tag="PC479_000295"
     CDS             25056..25961
                     /locus_tag="PC479_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2802884.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            25970..27154
                     /locus_tag="PC479_000296"
     CDS             25970..27154
                     /locus_tag="PC479_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2802885.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            27269..28570
                     /locus_tag="PC479_000297"
     CDS             27269..28570
                     /locus_tag="PC479_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2802886.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            28524..29567
                     /gene="holA"
                     /locus_tag="PC479_000298"
     CDS             28524..29567
                     /gene="holA"
                     /locus_tag="PC479_000298"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2802887.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(29754..30020)
                     /gene="rpsT"
                     /locus_tag="PC479_000299"
     CDS             complement(29754..30020)
                     /gene="rpsT"
                     /locus_tag="PC479_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2802888.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            30598..31119
                     /locus_tag="PC479_000300"
     CDS             30598..31119
                     /locus_tag="PC479_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2802889.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            31153..31929
                     /locus_tag="PC479_000301"
     CDS             31153..31929
                     /locus_tag="PC479_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2802890.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            32271..33224
                     /locus_tag="PC479_000302"
     CDS             32271..33224
                     /locus_tag="PC479_000302"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2802891.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            33242..34261
                     /locus_tag="PC479_000303"
     CDS             33242..34261
                     /locus_tag="PC479_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2802892.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            34292..34543
                     /locus_tag="PC479_000304"
     CDS             34292..34543
                     /locus_tag="PC479_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802893.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            34825..36630
                     /gene="lepA"
                     /locus_tag="PC479_000305"
     CDS             34825..36630
                     /gene="lepA"
                     /locus_tag="PC479_000305"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2802894.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            36779..38116
                     /locus_tag="PC479_000306"
     CDS             36779..38116
                     /locus_tag="PC479_000306"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2802895.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            38349..39662
                     /locus_tag="PC479_000307"
     CDS             38349..39662
                     /locus_tag="PC479_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2802896.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            39834..41009
                     /locus_tag="PC479_000308"
     CDS             39834..41009
                     /locus_tag="PC479_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2802897.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            41112..42131
                     /gene="hrcA"
                     /locus_tag="PC479_000309"
     CDS             41112..42131
                     /gene="hrcA"
                     /locus_tag="PC479_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2802898.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            42161..42781
                     /gene="grpE"
                     /locus_tag="PC479_000310"
     CDS             42161..42781
                     /gene="grpE"
                     /locus_tag="PC479_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2802899.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            42873..44720
                     /gene="dnaK"
                     /locus_tag="PC479_000311"
     CDS             42873..44720
                     /gene="dnaK"
                     /locus_tag="PC479_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2802900.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            44931..46085
                     /gene="dnaJ"
                     /locus_tag="PC479_000312"
     CDS             44931..46085
                     /gene="dnaJ"
                     /locus_tag="PC479_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2802901.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            47051..47986
                     /locus_tag="PC479_000313"
     CDS             47051..47986
                     /locus_tag="PC479_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2802902.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            48022..49395
                     /locus_tag="PC479_000314"
     CDS             48022..49395
                     /locus_tag="PC479_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2802903.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(49583..50350)
                     /locus_tag="PC479_000315"
     CDS             complement(49583..50350)
                     /locus_tag="PC479_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2802904.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(50380..51489)
                     /gene="ugpC"
                     /locus_tag="PC479_000316"
     CDS             complement(50380..51489)
                     /gene="ugpC"
                     /locus_tag="PC479_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2802905.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            51747..52484
                     /gene="cas6"
                     /locus_tag="PC479_000317"
     CDS             51747..52484
                     /gene="cas6"
                     /locus_tag="PC479_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2802906.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            52489..53964
                     /locus_tag="PC479_000318"
     CDS             52489..53964
                     /locus_tag="PC479_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802907.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            53984..54997
                     /locus_tag="PC479_000319"
     CDS             53984..54997
                     /locus_tag="PC479_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HDF2802908.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            55013..55810
                     /gene="cas5"
                     /locus_tag="PC479_000320"
     CDS             55013..55810
                     /gene="cas5"
                     /locus_tag="PC479_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2802909.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            55837..58245
                     /locus_tag="PC479_000321"
     CDS             55837..58245
                     /locus_tag="PC479_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2802910.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            58686..59633
                     /gene="prmA"
                     /locus_tag="PC479_000322"
     CDS             58686..59633
                     /gene="prmA"
                     /locus_tag="PC479_000322"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2802911.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            59652..60410
                     /locus_tag="PC479_000323"
     CDS             59652..60410
                     /locus_tag="PC479_000323"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2802912.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            60412..61710
                     /gene="mtaB"
                     /locus_tag="PC479_000324"
     CDS             60412..61710
                     /gene="mtaB"
                     /locus_tag="PC479_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2802913.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            61753..62103
                     /locus_tag="PC479_000325"
     CDS             61753..62103
                     /locus_tag="PC479_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2802914.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            62266..62445
                     /locus_tag="PC479_000326"
     CDS             62266..62445
                     /locus_tag="PC479_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2802915.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            62475..62918
                     /locus_tag="PC479_000327"
     CDS             62475..62918
                     /locus_tag="PC479_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2802916.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            63029..63526
                     /locus_tag="PC479_000328"
     CDS             63029..63526
                     /locus_tag="PC479_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2802917.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            63719..64282
                     /gene="raiA"
                     /locus_tag="PC479_000329"
     CDS             63719..64282
                     /gene="raiA"
                     /locus_tag="PC479_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2802918.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            64495..64731
                     /locus_tag="PC479_000330"
     CDS             64495..64731
                     /locus_tag="PC479_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2802919.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            64744..66087
                     /gene="yqfD"
                     /locus_tag="PC479_000331"
     CDS             64744..66087
                     /gene="yqfD"
                     /locus_tag="PC479_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2802920.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            66026..67141
                     /locus_tag="PC479_000332"
     CDS             66026..67141
                     /locus_tag="PC479_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2802921.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            67198..67659
                     /gene="ybeY"
                     /locus_tag="PC479_000333"
     CDS             67198..67659
                     /gene="ybeY"
                     /locus_tag="PC479_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2802922.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            67665..68384
                     /locus_tag="PC479_000334"
     CDS             67665..68384
                     /locus_tag="PC479_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2802923.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            68503..68904
                     /locus_tag="PC479_000335"
     CDS             68503..68904
                     /locus_tag="PC479_000335"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2802924.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            68968..69861
                     /gene="era"
                     /locus_tag="PC479_000336"
     CDS             68968..69861
                     /gene="era"
                     /locus_tag="PC479_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2802925.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            69873..71252
                     /gene="mgtE"
                     /locus_tag="PC479_000337"
     CDS             69873..71252
                     /gene="mgtE"
                     /locus_tag="PC479_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2802926.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            71325..71480
                     /locus_tag="PC479_000338"
     CDS             71325..71480
                     /locus_tag="PC479_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2802927.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            71446..72219
                     /gene="recO"
                     /locus_tag="PC479_000339"
     CDS             71446..72219
                     /gene="recO"
                     /locus_tag="PC479_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2802928.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            72212..72811
                     /locus_tag="PC479_000340"
     CDS             72212..72811
                     /locus_tag="PC479_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2802929.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            72971..73849
                     /gene="glyQ"
                     /locus_tag="PC479_000341"
     CDS             72971..73849
                     /gene="glyQ"
                     /locus_tag="PC479_000341"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2802930.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKDIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            73849..75915
                     /locus_tag="PC479_000342"
     CDS             73849..75915
                     /locus_tag="PC479_000342"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2802931.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(76081..77628)
                     /locus_tag="PC479_000343"
     CDS             complement(76081..77628)
                     /locus_tag="PC479_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2802932.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            77908..78675
                     /locus_tag="PC479_000344"
     CDS             77908..78675
                     /locus_tag="PC479_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2802933.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            78958..79155
                     /locus_tag="PC479_000345"
     CDS             78958..79155
                     /locus_tag="PC479_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2802934.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            79184..80260
                     /gene="vorB"
                     /locus_tag="PC479_000346"
     CDS             79184..80260
                     /gene="vorB"
                     /locus_tag="PC479_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2802935.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            80263..80982
                     /locus_tag="PC479_000347"
     CDS             80263..80982
                     /locus_tag="PC479_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2802936.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            80995..81534
                     /locus_tag="PC479_000348"
     CDS             80995..81534
                     /locus_tag="PC479_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2802937.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            81691..82749
                     /gene="buk"
                     /locus_tag="PC479_000349"
     CDS             81691..82749
                     /gene="buk"
                     /locus_tag="PC479_000349"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2802938.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            82767..83660
                     /locus_tag="PC479_000350"
     CDS             82767..83660
                     /locus_tag="PC479_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2802939.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            83690..84898
                     /locus_tag="PC479_000351"
     CDS             83690..84898
                     /locus_tag="PC479_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2802940.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            84922..86181
                     /locus_tag="PC479_000352"
     CDS             84922..86181
                     /locus_tag="PC479_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2802941.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        34528 a         9059 c        15256 g        27692 t
ORIGIN      
        1 aaaggagtag tatttaaaat aaatacaaat aaaaaattat tattaaaatg ataattaata
       61 tcaataagta ttgacaaaat tatttctaaa tactattata tataatataa ataataatga
      121 aaatcaatat caaataaatg ggaggaaatc taatgaactt attagtgatt ggtggtcatg
      181 aaagaatgga aaaagattat atggttatgg ccaagaaaaa aggttataag acaaaggtat
      241 atactaccat gtcatctaaa ttaaataatt ctataggaaa accagatgca gttgtcatac
      301 ttacatcaac tgtttcacat aagatgtcta gaacggttga gtctcaagct aaaaaacaag
      361 atatattaat cgtaaggcat aaaaatagta gtaaggtagc atttaatgaa tgtctagata
      421 tggttgatga atgtctggga aattgttcaa aatgtaagct taaagacaaa caaaaacaaa
      481 aaaatatata ggtataaaaa atacataaac ataaaattaa aagtactgag ataatagtta
      541 taattattat ctcagtactt ttagatttat aaggataagt atagtataaa ttttttatta
      601 taatttccaa ctacttgtta ctttttcata ttttttttac ttctagcttt ttttcttttt
      661 ttcttcttta tatttgaagc tattataaat aaaactaaaa gcactagaat acctgcaata
      721 atcttaacaa aagtcattaa aacattattt tcagtcaaaa atggaagact gctatcaaga
      781 ttattttttg caattaagtc tatactgttt tctagtttac cattattata tacatttaaa
      841 gtaccaacct tagctccttt ttttataggc aaatcaattt tatttaactc tgttttggta
      901 tcataattgc cttgagattc atctttttct aagaatatcc tataactatt ttttggttca
      961 taaatcaatt catgttgttt tgtaaacaat acttttttag tatctgtgta tttttcttta
     1021 tcaacaatag tagcacactt aaagttatca aatccataat caatcagaat acgtgagtct
     1081 aaatataaat cagcattagt tgaattaaat acagcaacga taactcttct gccatctttt
     1141 acagcactcg ataaaagaca tttaccagca gcatcagtat atccagtttt tataccatca
     1201 actatatcat attttatatt tataggttga cctttgtatg taatcttatc atgtgatgtc
     1261 aaaaacttat tagtgtttac aaagtatctc tcataaggat atgcttttgt agcttcaaat
     1321 ttaagtgact tagtttttac aatttgtcta aaagtatcat tattcatagc ttctctagct
     1381 atgagagcca tatcatgagc tgttgtgtag tgatttgggt ctggtaatcc atgaggatta
     1441 ttaaaatgag tattagtagc tccaattttt tttgcctcag agttcatgag gtcaataaat
     1501 ttttgtacat tgccaccacc aacatatcta gctaatacaa aagcagcatc attagcagaa
     1561 tgaaccaaaa gagcatctag taactgcttt acagtaaaag attccccatt ttttagatac
     1621 atactagagc catctatttg aggtaaatct ttaatttcta ttacttgatt taaatcttta
     1681 acctccttta gaactaaaca agctgtccat actttagttg ttgaagctgg ataaagctta
     1741 gaatttccat ccttattata tagtattttg ccagactcat agtccattaa aactgcatgt
     1801 ttagcaacta tagctggttc attatctgca aaagagatat tagagcttcc taaaaatgaa
     1861 gtaaatatca ataaacagat taataaaagt gataaatttc ttttcattgt gagcctccaa
     1921 attttaaatt catcttttag tatagcatat atttgtcaat aattaaaaga aaggagtgat
     1981 taatatatga aaaaaaaggt aaaatttatt tcgttttttg ttttatttat tttaataata
     2041 tcatcaataa tcgtttataa atcgcttatt ttcaaagatg atatttatgt tgtaagtaaa
     2101 aaaaatttac ctaatgaaaa aacacatgtt gtagagaatg aaattaaagg tgaagataat
     2161 aatcacagta aagtaactaa agacatagat aaaaattttc aaaataataa aataactata
     2221 tacataagtg gagcagtaaa tagacctgga attgtaacta tagagtcaga caagagactt
     2281 tatgatgctg tagaattact aggaggaacg acaaaagaag cagatttaaa tggagtcaat
     2341 ctatcagtaa gattagaaga tgaacaacat tacataatac ctaaaatagg tgaagcaaca
     2401 tctgttacaa gtaatgatga ttccaataaa ctaaatcaga agaatgagtc aagaggtgag
     2461 cctaaaaata aatccaataa aaatatatct aacgaatcaa aagtaaatat aaatgtagcg
     2521 acgatagaag agttggattc tctacctggg gttggagaag ctacagccaa taaaatttta
     2581 caacacagag aagaaaatgg acagtttagt tcaattgagg aaataaaaaa tgtaaatggt
     2641 attggagata aaaagtatga aaatataaaa gatttaattt gtgtagacta aaatataata
     2701 caaaacttac atatagatat gttattataa atatgtatgt atatgtgtgt acatgtggaa
     2761 atttagtaaa ggaggcactt ttatgaaaaa agaacaaaaa aaacttacag agatgacaac
     2821 agcaggtggt tgagccgcta aaatagggcc agaggttctg gcatcagttt tatctcaatt
     2881 gcctaaaaat gataatatag aaaatttatt agtaggacta gatacagcag atgatgctgc
     2941 agtttataaa ttaaatgatg atatggcact tatccaaact ttagacttct ttacaccaat
     3001 ggtagatgac ccatacgtat ttggtcaaat tgctgcatca aattcactat cagatgtata
     3061 tgctatggga ggcaaacctt tggttgctat gaatattgtt tgttttccat catgccatga
     3121 tatggatgta cttgcagaaa tattaaaagg tgggtttgat aaagtgaaag aaagtggagc
     3181 acttttggtt ggagggcata ctgtagatga caaagagcct aaatatggtc tttcagtttc
     3241 tgggattgta agtccaaata aagttttatc aaatgctact gcaaaacctg gtgataagtt
     3301 aattataaca aaaccaattg gagtgggtgt attaaataca gccatgaaag aaggtatggt
     3361 agaacaacat atagcagata aagtaataga aataatgata catttaaata aatatgcagc
     3421 aatgagtttt gagaaatttg atgtaaattc ggttacagat ataactggtt ttggtctact
     3481 tggtcatact ttagaaatgg ccaaagcttc tgaagtaagt attgaaattg aatcaaaaca
     3541 tgtaccaatt attgaaggtg caatagaaat ggcacaaatg ggaattatac ctgctggtat
     3601 gtataaaaat atgcattatg tatctaaaga tgtagaagtt gtaggaaata tagaagttgc
     3661 agttcaagat attttatacg acccacaaac atcaggaggg ttgttaattt cagtaaaaga
     3721 agaacttgca gaggagcttg taaaagatat gaaattaaat ggagcaatag aagctaaaat
     3781 tataggttct gtagttccaa aaggggaaaa atatattaca gttttataaa atattataga
     3841 gaaagagtga ttgttttgag caaaagagaa ttgtttgcaa tgttgccttc agttgatgaa
     3901 gtattaagtg ataatagaat tgtagaaata ataaatgaat atcctagaag tttagtttta
     3961 gaatctgtga gagaagtaat agatttaaaa agacaattta tattaagatt aaaagaagat
     4021 gcatcaaact ctgtaacaat agagtttggt gaaataatag aatctgcaat agagagagtt
     4081 aagttaaatt actcattatc tttaaaaaaa gttataaatg caactggtac agttattcat
     4141 acaaatttag gaaggtcttt acttagtgaa gatataaaag atgaattatg gtgtgctgct
     4201 tcaaggtatt caaacttaga atacgatttg gataatggag aaagaggttc aagatactct
     4261 catttaacta gtacaataaa aagacttact ggagcagaag atgtactagt tgtaaataat
     4321 aatgcagcgg cagtacttct tgtacttagc acaatggcta aaggaggaga agcaattgta
     4381 tctcgtggag aattagttga agtaggtggt tcatttagga ttccaagtat aatggctctt
     4441 agtggtgcag aattagttga agttggttca actaataaaa ctcatttgaa agattacaag
     4501 gaggccatta cagaagatac aaatgtattg atgaaggttc atacaagtaa ttatagaata
     4561 atgggattca cagagagtgt ttctatagaa gaattggtta atttaggtaa aaaatataaa
     4621 ctacctgtaa tagaagactt aggaagtgga gtttttatag atttatcaaa atatggttta
     4681 tcttatgaac caactgtatt ggattctatt cgacaaggtg cagatgtagt aacttttagt
     4741 ggagataaga tgcttggagg tccgcaagca ggtattatag ttgggaaaaa agagtatata
     4801 gaaaaaatga agaaaaatca acttactaga gcacttagag ttgataagct tacaatatgt
     4861 gctcttgaag caacattgag aatgtattta gatgagacaa aagctataga aaatatacct
     4921 actttaaaaa tgttgacata taaaattgaa gaattagaag taaaagcaaa taaactattt
     4981 gaaaaaataa ctgctttaaa cttaaatgca aacataaata tagaagatgg attttcacag
     5041 gtgggaggag ggtctatgcc tcttgaaact ataagtacaa aggtaatatc aataactcct
     5101 gaacatatga atgtttcatc tttagaaaaa aaactaagac ttagtgaagc acatataata
     5161 gccagagtgt atgataataa atatgtacta gatgtaagaa ctatatttga tgatgaattt
     5221 gatgtaatag ttgaggaatt gagaaaagca tttaattaaa taaatttgag ttaataaata
     5281 ttaaatttag aagtataagt atgaagtttt gattaaaaaa tagctacaca cacttgtgac
     5341 tttagtcata gcaggtttag agggggcaaa aaatgaaaaa tgtagtaatt ggaacagcag
     5401 gtcatattga ccacggaaaa actactctta taaaagcatt gactggaaga gagactgata
     5461 cgttagatga agaaaaaaag agaggtattt caataaactt aggatttact tattttgatt
     5521 taccaaacaa tagaagagct ggaattgttg atgtcccagg acacgaaaaa tttataaaaa
     5581 acatgttagc tggagcatca ggaattgata tagttttact tgttgtagca gcagatgaag
     5641 gggttatgcc acagacagtt gagcatttag atattctaag ctttttaaat atcaaaaatg
     5701 gaatagtggt tctaactaag tctgatactg tagatgatga atttagagag ctagtaaaag
     5761 aagatatttg tgaaaaaata aaaggaactt ttcttgaaga tgctgaaata attgaagttg
     5821 attctatatc taaaagaggt atagacactc ttattgaaaa aatagataaa atgagtgatg
     5881 atatagaaga taaaaatgaa aactctccag caagacttaa tatagacaga gtattttcaa
     5941 taaaagggtt tggtactgta ataacaggca ctttaataga aggaaagata agtatagaag
     6001 atgatttggt tatatatcca caggtattaa aaacaaaaat aagaagtata caagttcatg
     6061 gagaaaataa agatacagct tatgcaggtc aaagaacagc aatcaatata tcaaatgtaa
     6121 aagtggatga tataaaaaga ggagatgttt tagctgcacc aaactcattg gaggaatcaa
     6181 tgatgttaga tgtaaaatta tcattggtaa atcatttaga taaaggatta aagcactggg
     6241 atagattaag attgtatcat ggaactagag agatactttg tagagcagtt ccactagata
     6301 aagaattaat tgaaagtgga gaaagtggct atgttcagtt gagacttgaa gaaagcatag
     6361 tatctaagaa aggtgatacg tttgtagtga gacgttattc tccaatggaa actataggtg
     6421 gaggtgtaat aattgaccca tctcctaaaa aacataagaa atttgatgaa aaagttatag
     6481 aagcattaaa aataaaagaa aaaggcgaac taaaagatat aatagaagag tacttaaaaa
     6541 ggaatttaaa aaattatcca aatataaaag aaataatgag ctatagtggt gctcatgaag
     6601 aagatgtaaa aagagcgctt gaaacactta tatcagaaga taaagtattt attataggaa
     6661 atatgtatat gcatataaat caatacaata aattaaaaga aaatacaata aagcttttaa
     6721 gtgagtacca taagaagtat aggcttagaa aagggatact aaaagaagaa gtaaggtcta
     6781 agatagagag taattttaag actagggaga tggatatttt attagaaaaa ttgtctacag
     6841 aaaatgctat aaaaatagaa aataatatag tatctctatt agattttgag gttattttaa
     6901 atgataagca aaaagaaata gcaaaaaaaa tagaaaaaag attaaaatca tgtggagttt
     6961 cttctatact gacaatagat gaagtttctg aaggaaatca taactatgct gaagtattgg
     7021 agtcaatgat aggtaataaa gtagagaaat tagatgattt gtatataatg gacaaagata
     7081 tttatgaaaa tgcaaagaac attttgataa attatataaa agaaaataaa gaaataacat
     7141 taggagaata cagagatttg attgattcta gcagaaagaa ttgcatgatt atattggaaa
     7201 acttcgatag aaacaaaata actaaaagag tagaaaacaa aagaattttg ttttagcaga
     7261 aaatgtctgt ttttgcttta aggtataaag aaggaattat aatgtcgagt atcgtataat
     7321 taattagtat aaaatattaa ttaagaggaa gtgtaaagta ttgaagaata taatacctga
     7381 tgtaaaacca gatataaaag aaagagaaga catgagcctt ttatttataa taatgggctc
     7441 agtactttta ttatttatta taaaagatat agagaaatat gaaactttat tacatgccat
     7501 atatacgata tttttagtag ctacaggagt aattatattt aatactacaa aatttaaagt
     7561 agattcattt tctacatttt taggattaat ttttatagct actggagtat tggagtgtat
     7621 atatttattt aattccttgg ggattaaaac taagtctata atggagatta acatcacaat
     7681 atcagcaata acagatttat ttcctatact gggtgtatac ttaagtttta aatttgttaa
     7741 ggataacaaa caaatttatt caagtgtaat tttatttata gcaactgcta cattaacaat
     7801 aacatcttta ttcataatat caggaatttg tgactatctg ggaaatggta aatcacaata
     7861 tgctttagga gttataatat ctatattcat aataattatt tctatcatta gtggaattga
     7921 attaaattca agttcaaata aaggtaaatg ggaatatgat gaaaaaaaga tttttaacag
     7981 aataatagta ataataatct tatcaagagt tccaaatcta ttgcatatag ttattgataa
     8041 tagaaatgta gagaggatat tatcacaaat aataataaat gtagccttat attatttata
     8101 taattacata gtatctaaga atattaagaa aacagcactg gaattgcatg atacgaatga
     8161 agaattaaca aaaaaaacgg aaagtttaaa agaaaagaat aaaaaattaa tctatgagac
     8221 agaaaagata gaggaactta agggaatact tatgaaaaga gaatctaggc tacaatctac
     8281 attagatgta gcagtaaata gcatagtagt atttagcaaa gatggagaaa ttacatatgc
     8341 aaataagagc tttagaaata cttttggaga ctataaagaa caagataaat tgactgataa
     8401 aataaaaaac ttcaatgatt tgatagaaag cattcattat gtgtttgttc atgagaagaa
     8461 tgcagagaaa cttattagta caagcgataa taaagtttat caagctatat ttgcaccttt
     8521 aattattgct tcacaaacag aaggagtatt gtgtgtacta gaagataaga ctaagaaaaa
     8581 ggagtacgaa cgcaaactta ttgaagcaaa taagagaagt aaagattttt tagaaagtat
     8641 tggagatggc atagtagtgc ttgaaggtaa taacaaaata tatgttaata atgcatgtag
     8701 ggaaattttt aaaaataatt tagagagtat tgatttttca ttagtatgta aaagtgaaga
     8761 aagtatagaa aaaagatatg taatagatgg tgaagtaaag tatgtagaga tgagtttttc
     8821 tcagtatact aatgctggta ctaataaaac tattattgta atacgtgata caacctctcg
     8881 aaaaatatct caaataaaac ttgaggaaag tcaatcatca tattcaagat ttatagatat
     8941 attacctgat ggcatatgct tattaaaaga agatttaagt ataaactatg caaataagtc
     9001 gtttttagat atgctagctt ttacaaacat agatgatata aaagattcta atattaaact
     9061 attaatgaat gcgaatactg aagaaaaaat gaaatttacg gataatatgg ctaaagtttt
     9121 aaatgaaaat agaagtatac ttttactgga acatgaattg ataaatagtt atgatgatat
     9181 agtagaagta gaattaagtg cattaccttt tgctatatac aatactagat acataatgct
     9241 tattataaaa gatttagtac ataagaaata ttccgagcaa gcagaaaaag aactgcttga
     9301 aagatttaaa acagataaaa tcaaaacgga gttttttgca aatatgtctc atgaactaaa
     9361 aactccacta aatgttatat ctagttcaaa tcaactggta gattctttct atagaaatga
     9421 aaagataaaa gattacaata acaatattaa atctcatgtg gatttagtta gacaaagttc
     9481 ttataggctt caaagattaa taaataacat tatagattta acaaagatgg agtctgggtt
     9541 ctatacacta aaacttgcga aatacaatat tgtatctgta atagaagact tatttatgaa
     9601 tatagaagag tatgcactta gaaaagacat aaaaattctt tttgatacag accttgaaga
     9661 aattaatgtt tatatagaca aggttgaaat tgagagaatt atgttgaatc tattgtcaaa
     9721 ctgtattaag tttactgata atggaggctg gatatatgta agtattcatt ataaaataga
     9781 taaagtaatc ataagtgtaa aagacactgg agttggaata ccacaagata aattagagtt
     9841 gatttttgaa gaattttcac aagttgataa gactttatcc agaaatactg aaggaagtgg
     9901 aatagggctt gctatagtta agaatctagt aagtcttcat ggtggagata tagaagtagt
     9961 aagtgaagtg aataaaggca cagagttctt aatttcactt ccaatgaagg gatttagtag
    10021 tgaacattac acagaagata agagaatata taatattcaa gaaaaaataa aaatagaatt
    10081 ttctgatata tattattaaa aatgattatg agcaaaaata aaataaaaat actatttgtt
    10141 taaatattta agttgtaaga aataggctat cataaggtaa attattatga tagcctattt
    10201 ttagctaggt tttatatcta tatgtgagaa catataatct ctaaattacc ttcaaaacta
    10261 aaatctccaa agtcgtttgg cagtataaaa tgcataccct tttttaaatt atattctaca
    10321 gaattgtgta taagttttcc atttccatcc aacacactgt ataaagaaaa aggtgtattg
    10381 tgtgtaaaat tacattttcc aaaaacatct aatttataaa cactaaaaaa ttcgtttgac
    10441 acaaatgttg tgcatttaaa gtctgatgtt gatacgattt tatagtcagt atcgaaattt
    10501 atatgaggaa cattcgtaac atctattgat ttttgaacat gaagttcacg cttattacct
    10561 gaattgtcag ttctatcata atcatagact ctataagtag tatctgagtt ttgttgagtt
    10621 tctaaaatca atgttccttt gcatatagca tgtatagtgc cacttggaac atagaaaaag
    10681 tctccctttt ttatattaac ctttctaagt agtttgtccc attcattatt atttaccata
    10741 tttacaaatt ctttatgaga ttttgcatta tgacctatta taatttctgc atcatcagaa
    10801 caatctatta cataccaaca ctctgtctta cctaactctc cattttcatt ttttttagca
    10861 tattcatcat ttggatgaac ttgaactgat aaattatcat ttgcatccaa tatttttgta
    10921 agtaaaggaa acttatcacc aggtgtgttt ccaaataatt ctctattttt attccacaat
    10981 tcagatagtt ttttaccttt atattttcca ttttctatta agcaatctcc atttttatga
    11041 gagcttatag cccaacattc accagttgtt ggcgaatcta tttcataatt aaatttatct
    11101 ttaagtgcag taccacccca tattctatcc ataaaaatag gttttaaaaa taatggttcc
    11161 ataatcctgt tcctcctatg atattaaatt tatcctacaa ataattatat ataaatagat
    11221 tataaaatca atagtaaaaa agttttgtcc cttgaaatga cataaagtat aaggagttat
    11281 taaataagat aaactatgat atataatata aagttctaaa ttggagtgat aattatggag
    11341 ttgtttaagg cagaaagcaa aaaatataaa aaagaatttt taaaattatt ggagcaatgg
    11401 gttagtatac cttcatttta tgatagaaaa actgtatcta aagacatgcc ttttggaaaa
    11461 ggagtatatg atgcattaaa ctggtttgaa aatctaggta gagaaaacaa tttcaaagta
    11521 aaaaatatag ataatcatgc tgttcaaatt gaatatggaa atggtaaaga gtatgtagat
    11581 atatttggac attgtgatgt tgtaaatcca ggtgaaggat gggatagtga accatttaaa
    11641 ctaaatatta taggtgataa attagttgct agaggtgtat ctgataataa agggcctatg
    11701 atagtgaatt ttttagcact aaaaatgata aaggatttag atattaattt aaaaagaaag
    11761 gttagattaa tagctggggg aaatgaagag agtggattta aatgtataaa gcattattat
    11821 agcaaagagc catatggtgt atgtggattt actccagatg ctaaatttcc tgtattaaat
    11881 ggagaaaaag gtggagcaat aataaagtta atttcaaata tagacgataa atctttatat
    11941 ataagtggtg gaatagagtt taatacaata ccagataaag tttatataaa gaatgtagaa
    12001 aaattgggta aagataatat atgttttgat attaacaata tatcgataaa ttatgataat
    12061 ggcaattata ttgtgtatgg aaaaggtggt cattcatcta aacccgaaaa atcaataaat
    12121 cctatattag ctacaattaa acttttgtca gaaaatattg atgaaaaatg gacaaaagat
    12181 ttatataagt tgataaatca agataatata aatggaaact tatttggttt aaatattgag
    12241 gggaaatgtg gaattttatc aatggtgcca acaataataa atatagtaga tggaaaatta
    12301 gaagtagttt taagcgttag atatccagaa atattaacca ttgaagatat aataaaaaaa
    12361 tttaatttgt atatggagca aaataatatt aataaatttg agcttattgg ggaaaattta
    12421 aagcaagcaa attatataga tagaaattca aaacttgtaa gaagtttgca tgatatatat
    12481 ataaagtatt ctggagattt aaaaaatgat gttagagtaa ctagtgcagg aagttatgct
    12541 tctgaaatga ataattcagt aatatttggt tgtgaatttc ctgatggtag ctttggcaat
    12601 gtccatagtg cgaatgaatt tgctagctta gatagattta taactgcaat aggtatatat
    12661 gcagaggcaa taattacatt atgtaataaa atataaatac aaaatgtccc ttatatagac
    12721 atattatata tacaattaaa attttacttg atatataatt gaaatcaaag agaaggtgat
    12781 acaatgataa ctaagaaaca tgcaataata gtaaagagtt taagcaataa agatggatat
    12841 atgacatcaa acgaattagc tatcaaattg gatgtgtcta ctaaaactat taaaagatat
    12901 atagcagatt taaatagtgt tttaagcaaa tatgatttgg taatagcttc ttctagaggt
    12961 ataggataca agttaagcgg ttctaaaaac aatatagcca gagctgttaa agaagcaaat
    13021 aaatatattg atggtttttt agatgactct gaagagtcaa gaatgtcaaa tataatatgt
    13081 atgcttatta atagaaatta tatgagtata gaagcaatgg cagaagaatt aaatctaagt
    13141 atagccgcta taaataaatt gtctagtaag cttaaaagaa aacttgaaaa gtatgactta
    13201 gttataaaat ctaaacctta ctatggaagc tatatacatg gtgaagaaat taatataagg
    13261 caattaatta ctgactatgc aataaaatta gatgaaaaaa gtaaagtgaa ggtgttttta
    13321 gatgacatta gtgaaaacga tatacactgt atagaaagta ttttagaaaa gcacttaaga
    13381 gaaaaagata caataatttc agataaagat tttaatcttt tgctttcaaa aataatcgtt
    13441 tctgtattta ggagtaagag aggtcatagt aacaatataa acttaatgga tacaagttat
    13501 agatttcata actactactt tatagaaaat ctaatgaaag aaatatctga taaaattgga
    13561 tttaaactta ttgaagatga agtaatttat atttcaaatt attctggtgt aatagcctat
    13621 aaaggtacac aaggaagaaa aaacacaagt gaaattgagg aacgaataag tagtgttata
    13681 agtagtgcac ttcaagatat attccttatt tcaggtagtg attatacaaa agatgatgag
    13741 tttatggttg caatctctga ccatataaag agatttttaa atagagctag agctaatgta
    13801 aaatcaaata atcctttact tcatcaaata aaagagaagt ttcctatagc ttttaattta
    13861 gccgtgtttt tatcaaataa actagaaact gagtttaatt taaaacttga tgaagatgag
    13921 cttggatata tagctatcca ctttgctgct tcaaatgaaa gaatgaaaaa aaatactagt
    13981 aagaaaattt gtatagtttg tcattatggg ataggaacag gacaacttat ttctgaaaag
    14041 ctaaaacaga atataagtga tttaagtgtt gtaggtgttt atccagttag atatctcgat
    14101 atggctataa gtcaagatgt tgatttgata gtatctaccg ttgaattaaa aggatatgaa
    14161 aaacctgtct tatatataga aaatatattt gatgatagct taattgaaaa cgtaaacaaa
    14221 gccttttatg aaaaggaaga aagaagaaag ataataagca acatgtttga tgaaaaagct
    14281 tttttcagta taaaggctaa tactaaagaa gaggtcataa tttcactttc aaataaacta
    14341 aaagaaagag attttataga ggaaagttct ataaaaagta taatagatag agaaaatatt
    14401 tcatcaactg aaataggaaa tctagtagca ataccccaca caatagtgaa gggagataaa
    14461 aaatctataa taggagttgg aatacttgaa aatccaataa tttgggataa gcaagaggtt
    14521 cagctagtat ttatggtatt ttttaatact aaagaaaaac ataatttctc gatatttaaa
    14581 tacctttata attttataaa agatgaaggc ggagtaagag gaattattaa aatatgtgac
    14641 tttaataaat taatggcact gataggtaat taaggaggaa attaataatg ggaacaaaca
    14701 tatttaacaa agaatatgta tttttaaatg ttgatgctaa gtcaaaggtt gaagttttaa
    14761 aatttatatc aaaaaaagct aaagacttaa acttggcaga agatgagagc ttagtatatg
    14821 aaggtttaat ggctagagaa gaacaattta ctacaaattt aggagaatct atagcaatac
    14881 ctcacactaa aaatgatgca attgaaaatc cagcagtagt agtattgaag tttaatgaag
    14941 atgttgtttg gaatgaaggt gaggataagg ttaaattagc aataagctta cttatgccag
    15001 gaaaaagtaa agagaatatt catcttaaat tattatcatc tctttcaaga aaattaataa
    15061 ataaagaatt taaagattct cttttaaaaa gtgataatgt tgaagaaata tctaacttaa
    15121 taaatgaagc tttaggttta taattaaaca ctaaggttat aaaataaaaa aataattaac
    15181 taaaaatagg aggaatttga aatgaataaa aaattagtag cattatgtgc ttgtccaatg
    15241 ggactagctc acactttcat ggcagcagaa gcaatagagc aagcagcaaa ggcattaggt
    15301 tatgaagcaa aagttgaaac tcaaggtgct gatggtgtac aaaatgagtt aacaagagat
    15361 gatatattag gagcaactat gataatacat gcagtagcaa taacaccaga aggtatggaa
    15421 agatttgatg gttgtgaagt ttatgaagtt gaattacaag aagctataaa aaatgcagaa
    15481 ggtgttataa aagaaataga agaagattta ggaatataat aaatttattg tggaggaaat
    15541 agaatggcaa ttaagaaaaa agttataaac agttcaggtg cttctacaac aggaggaaat
    15601 aatgtaggaa aatctactcc atctagtcca actcaatcaa agggaaatgg aaaatggaaa
    15661 gaaataagta aacatataat gacaggtata tcatatatga taccagtttt agtaatgggt
    15721 ggacttatag gggccttatc tcaattaata ccatatgcta tattaggact tgacccatct
    15781 gtaggtatag ttgatgctat gaactcagga gaatttacag ggttcaagct tagtctttta
    15841 aatatagcac aattaatgtc aaatttcgga tttactttgt ttggatttgc gataccactg
    15901 tttgcagcat tttgtgcaaa ctctataggt ggaaaaacag ctttaattgc aggatttata
    15961 ggtggatata tagctaataa acctgtaggt gtaccacaat tcgttgatgg acaatggact
    16021 gaagttgttc cagttgcatc aggattttta ggtgcaatac taatagcatt tataatagga
    16081 tattttgtaa aatggttaaa taaatctata aaagtttctc ataactggtt agcttttaaa
    16141 acaacatttt taataccatt aatagcgtca ttagcttgta tggtattgat gatatttata
    16201 ataactccat ttggtggatt aattaatgaa agtatgaaaa acttcttaac agcagcagga
    16261 gcagctggag aatatgtata tgcaacagct ttggcagcag ctacagcatt cgacttagga
    16321 ggaccaataa ataaagcagc aggattcgtt gcacttggtt taactacaga gaatgtactt
    16381 ccaataacag caagaacaat agctatagtt gttcctccaa ttggattagg gttaacaaca
    16441 ttattagata aaagattagt aggaagaaga gtatatgata gacaattcta tcaagcagga
    16501 aaaacttcta tattcttatc atttatgggt atatctgaag gagcaatacc atttgcactt
    16561 gagagaccag gttttgttat acctttaaac atagttggaa gtgtaatagg agctattaca
    16621 ggtataattc taggagcaat tcaatggttc ccagagtcag ctatatgggc atggcctcta
    16681 gtagataact tatttggata tataataggt atagcagttg gtgctatatt catagcagta
    16741 ggaaatatat tctatagaaa taaattaata aaagatggta aacttgttgt agattatatc
    16801 gactagtatt taatttttac tatctataaa taactttggc tggtaatatg gttttaatca
    16861 atattaccag ccttacctat atgattaatt aaaggcggtg gagataagat atggataaga
    16921 tagaaaaagt aagagagtat tttagagaat atgatataga tggatttctt ataaatagtc
    16981 ctacaaacaa gttttatgtt ggaaatttat ttagctcttc aggttatgtt tttataacga
    17041 aagaatcaca atatataata gtagatttta gatattttga agaaattaaa agaaaatcta
    17101 gtttgtttaa tgtagtatta atggacaaaa ctagaactca ttttgatata attaatgata
    17161 tttgtagaga gcagaatata aaggaaattg gatttgaggg aaatgaagta tcttttgatt
    17221 tatatagaag tatgagtaat aagttaagtg caaccttaaa atcagttgat ttatctacat
    17281 taagggaaac aaaaaatgaa gacgaaataa aatatataaa aaaagcttgt gaaatagttg
    17341 atgctacatt ttatcatata gtagacttta taaaggttgg tatgactgaa aagcaagttg
    17401 aaaatgaaat tgttagaatt ataaaagatt taggtggtca aaaagaatct ttcgatacaa
    17461 tagttgcatc tgggcttaga ggagcattac ctcatggaaa agcaagtgaa aaagttatag
    17521 agtatggaga ttttgttacc tttgactttg gtgctaagta taataattat tgttcagata
    17581 ttaccagaac catttgtatg ggtactataa ataaagagtt agaagaaata tataatatag
    17641 taagaaaagc taatgaagag tgtataaggg ttttaagacc gggaatgact actggagaga
    17701 tagataaagt tgcaagagac ataattggtt catatggata tgctggtaat tttggtcata
    17761 atctgggtca tggtgtagga attatggttc atgaatatcc tgcattagct cctgaatcaa
    17821 atgaagtatt aaaagaagga atgattgtaa ctatagagcc tggtatatat gttccaagtc
    17881 ttggaggcgt aagaatagaa gatgatgtac ttataacaca agatggatgt atgagattga
    17941 ctacatctac taaggattta atagttgtaa gctaaaaata aattagataa atattttata
    18001 acaataagaa aagaagaggt aaatttaaat gtataatttt gatgataaac cagatagagt
    18061 aagtgaaaag tgtagaaaat gggatttaaa tattataaga gataaatttg gagatattag
    18121 agaagatttt ataccaatgt ggattgctga tatggatttt aaaataccaa cagaaataga
    18181 aaacaaattt attgaagctg taagaagagg tgtatttgga tatacatact gttatgatga
    18241 attttatgat tcagttattg gttggcaaaa aaatatgcat gaggtaaatg tagaaaaaga
    18301 atggattaca ttaacttatg gaactgtatc aacacttcat tatgtagtac aagctttttg
    18361 taagaaaggt gatagtataa tattaaatac accagtttat gacccctttg aatcatcagc
    18421 aaaaaaacaa ggtgtaaatg taatttgtaa tacattagat gttgtaaatg atagatatta
    18481 tataaatttt gataaattag aagttcaaat taaagaaaat aagcctaaat taatgatgtt
    18541 ttgtacacca cataatccat ctggaaggat atggacaatt gaagaaatga caagggtagc
    18601 aactatatgt aaagaaaata atgttatttt ggttgcagat gaagtacatg cagagcatat
    18661 tcattatggt acatttaatt caatacttaa gattggaaaa gagcttttag aaaatgttat
    18721 tctattgaca tcaccaaata aaggatttaa tcttggtgga ttaaagactt cttattcaat
    18781 agttataaat aaggatataa gggacaaatt tagaaataaa ctaaagcaaa attctataac
    18841 ttctccaaat gtgtttggaa taataggatt gataaccgct tataatgaat gtcatgagtg
    18901 gttaagtggt gtaaatgaat atataaaatc aaattatgaa cttttagaaa cttgggtaaa
    18961 taaatataat aagataaaat tgatgaaaat ggaatcatct tacttagctt ggatggatat
    19021 aagtggatta ggtattagtg caagtgagtt tacagataaa ttggctatag atacaggagt
    19081 attattagaa gatgggagtc attttgtaaa agatggtgag aagtatgtta gaataaattt
    19141 aggaactcaa aaggaaaatg taattgaagc tttaaataga atggatttat ttttaaagtc
    19201 tttataaaaa tttggttgaa ttagtatttt atataaattt aaactattaa tatggtatat
    19261 acataagtag aatttgtttt agattactta agtatatacc atattttata taaaaattaa
    19321 atttgcatta gtcatgtgat aactatttaa attcatatgt attttgtatg tataatagat
    19381 ataattatta tttaaactta ttgtttattt aatttttcaa tagaaaaaga tttatctgta
    19441 agtttaaaat tatcttttat acccttagtt aagtatactt tcttatcctt agttatgaat
    19501 atagcatcta cattatcaat tttattaatt aaattcatac ctttttcaag accaagacca
    19561 aatgtagaag tggataaggc atcacaatta atagatttat cagagattat tgtaacacta
    19621 cttaaattgt tttcaaaagg ataccctgta aatggattta acatatgatg atatattttt
    19681 ccgttttttt cgataaatct ttcataaatt ccagaagtaa caacagactt atttgtagtt
    19741 tctatgtttc ctatggattc accattttta ctagttgggt cttgaatacc tattttaaaa
    19801 ggtttatcat ttttaccatt ttctaaagta aatatatttc ctcctaagtt tactaaacct
    19861 ttttttacgt cctctgattt taaataggct acaattttat cagcagcata tcctttagct
    19921 atggcaccta aatcaatttt catattttct ttggttaatt ttatagattt atttttttca
    19981 tccaatatga tgttcttata atcaataaga ggcagaagtt tttgtatctc agaatcactt
    20041 ggaactttag ctttatctgt tccaatagcc cataaatttg ataatggacc aattgttatg
    20101 tcaaaatatc catctgataa tttggaatat tgtatagctt cttttacgac gaaaaatgta
    20161 tcatcagaaa ctctgacaaa ttttttacca gcattatcat ttattttaga aacatcactt
    20221 cctggtatat gagtgctcat tttgttgtca atatatctta aaattgaatc acattcattt
    20281 aagattttat cacttttgga ttcttttaca ttaaatacag taacttcgtt tactgtacca
    20341 aggtagtagc tagttttact atatttagta ttctggcttg aactcctaaa aaatgaaaat
    20401 aaaattaatg ctaaaatcaa tatcacaaaa ataaatgtga tttttttctt attcatggta
    20461 tattcctcct atgaaattgt gttattattt tattataaca taatttcata ggaggacaaa
    20521 aaatgaagaa aaaagacatt cttcttattt taggcatttt ggttgttatt acagcatgtt
    20581 atgggataat aaatgttata aattctaaaa atgctggaaa tattgagatt tatgtagata
    20641 ataaattgta taaaactgtt tcaataaatg ccaaagaaga atttaaaata gagaatagag
    20701 gggggtataa catagtaaag attcatgata aaggggttga gatagtagat gcttcatgtc
    20761 ctgataaggt ttgcgtacat actggattta taaacaagcc aagtcaaagc atagtttgta
    20821 tacctaatag agtgagcata aaaataaata caaatgaaaa gaatgataat caagaagata
    20881 taatttctaa ataaaataat aaaaataatt gaaaggaatt ggtgataatt atggttaact
    20941 taggaaacga ttgggatgaa ttattgaaag aagaatttga aaaagattac tacttaaact
    21001 taagaaaatt tcttatagaa gaatataaaa cacgccaaat tttcccaaat atgcacaata
    21061 tatatgaagc tttaaaacac acttcataca aagacacaaa agtcttaata ttaggacaag
    21121 acccttatca tggagataat caagctcatg gtttagcttt ttcagttcaa ccacaagtga
    21181 aaactccacc atctttatta aatatgtata aagaattaaa agatgattta ggttgtttta
    21241 tacctaataa tggatattta atgccttggg cagaccaagg agtgttactt ttaaatactg
    21301 ctcttactgt tagagcgcat gaagctaatt ctcataagaa caaaggatgg gaaattttca
    21361 ctgatagagt aatatctatt ttaagtgagc gtgaagaccc agttattttt gtactatggg
    21421 gaagtaatgc acgtaaaaaa gtagagttga ttgatacaag taaacattat attttggaag
    21481 cacctcatcc aagcccattg tcagcaagta aaggcttttt tggatgtaaa cacttctcta
    21541 agataaatga aattttgaaa aagctaggaa aagaacctat taattggcag atagaaaata
    21601 tataaaagta aaaggtaaca aaatgataac aaaacaacaa taaacaatga cattgttgta
    21661 actaatagaa atatgcttaa atagttgaaa atactatgta tgcatatttc tatttttttg
    21721 tcaagttgac aactcttgaa aaaatagtta caatttagtt acacaaaaaa cacaaataaa
    21781 aaaagaaact tacaaagcag aattttatga agcaaaatat atagtgtaaa aaatcttagg
    21841 gaggaaattt tatatgaatt ttaaaaaatt gagtaatgct aaagtctcgg taatggcagt
    21901 tgttttatca gttggaatat tatcgggata ctctgcgctt aataaacaag taacattagt
    21961 agtaagggga gaagaaagaa ctatatcaac atttagttct aatgtaggag accttttaga
    22021 atctcaaaac attagctatg atgcaaatga tatagtaact ttagatgaaa atacaaagtt
    22081 atctaatgga gataaaatag aagtaattga tgtaaaggaa aaaacagttg tagaaactaa
    22141 agacattcca tttgaagtta cagttgttga agatggctct ttattaaagg gtgattctaa
    22201 ggttaaagaa gaaggaaagt ctggtaaaag tgaattaata tataaagaaa cttatcacaa
    22261 tggaaagaaa gtagaaaagg aatttgtgaa aaaggttgtt aaagctgagc cagtaaataa
    22321 ggttgtaagc aaagggacaa aggtagaaat gcaagtagcc tcatcaaggg gagaaagttc
    22381 aagaagaatg gcttcatcat catctagtag cagtagtagt aacaatggta aaaatatgag
    22441 agtggtagca actgcatata ctggtcacag tataactgca acaggaacaa aacctaagtg
    22501 gggaacaata gctgtagacc ctaaggttat accatatgga actaaagttt atatacctca
    22561 atttgacatg gtgtttactg cagaagactg tggtggagca ataaaaggaa ataaaattga
    22621 tatctacatg aatgattcaa aaacagttta taactgggga agaaaaacta tagatatacg
    22681 tatattaaaa tagttaaata atttatattt ttactggaaa ttatattttg attatataga
    22741 gaagaaactc tttatagatt ctaaaaatat aatttccagt tttttatttt ggatatgtaa
    22801 taaaacaggt aaattttaac catgtataga taaaagctat ttaaattata atttactatc
    22861 atataaatct ataaaaattg ttgcaaagag tcaaaatata tgatattata gatttcgttg
    22921 agaaggattt cattatggga gtggatgggt gccggtgtgc cctctagtct tcaaaactag
    22981 tatgaggggt taagagcctc ttaggtgggt tcgattccca cgcattcccg ccaatatgta
    23041 aaaggtatct tttaattaaa agataccttt ttaaatttgt tttaagataa tttagtttta
    23101 atgatatata atattttgta ttatataatg taaattttat aatactatta ttgttcagat
    23161 aaaagtctag gagagtgaaa agatggaaaa taattttaca acgagaacta gttttctagt
    23221 tggagatgat gggatagaaa aacttaataa ttcaaatata atagtatttg gagttggagg
    23281 agttggtagt tttactgttg aagctttagc aagagctgga gtaggcaata ttactattgt
    23341 agattttgat gatgtagata taaccaatat aaatagacaa ataccagccc tacattcaac
    23401 tgtaggaaga tacaaagttg atgtcatgga agaaagaata ttagatataa atcctaatat
    23461 caatataaaa aaaataagaa gtctttataa taaagatact agtgacgaaa tactaactga
    23521 aagatatgat tatgttgtgg atgcgataga catggtaagt tccaaaatac atctaattga
    23581 aacttgtgaa aaaaaaggat tgaaaataat aagttctatg ggtatgggaa ataaattaga
    23641 cccaactaaa atagtcgtta cagatataca caaaacaagt acttgtcctc tagctaaagt
    23701 tatgaggaaa gaacttaggg atagaggtat caaaaagtta aaagtagtat attctactga
    23761 acaaccaata gaacttaaga agaaagtaat gaatggtaga aaagtaacac ctggtagtgt
    23821 atcatttgtc ccatctgtag gaggtttaat aatcgcatct gtaatagtaa atgaactatt
    23881 aggacaataa gttttaaata ttttaattta taaattttat atacaaattt gaaggctaga
    23941 aggatgaata taaagcaatt ttatattcat acttttagcc tagttatttt atattacctt
    24001 attttatgta gttgtagctt tattttttag attatgtatc tatttcataa ttatattttg
    24061 taaatataat ttctccatca ctataaaaaa caatatctcc tattctgtct gttctataaa
    24121 ttgatacatg gttttgtttt agattattaa taacctccct atgtggatga ccatattgat
    24181 ttttatatcc acatgatatt gcagctatat ctggactaat tttttttata aattctaaag
    24241 atgaagaaga attacttcca tgatgaccta tttttataaa attaatatta tttaatttaa
    24301 aagaatgtaa aatttctttt tcattttctt cttcagcatc acccataaat aaaaaagaat
    24361 tatcattaaa agttaatttg aaaactatag aatttaaatt agattcttct tgtatatatg
    24421 atggacttaa tacatatata tttatattgt tatctatgtt taaaacatca tttttataga
    24481 gatgctgaat agataagttt ttatccgtac aagaatttat tagattttga taagcctcac
    24541 tatcagttga ttgttcaggc atatatatgg agtttacatt aaattttttt ataatattat
    24601 caagacttcc tatgtggtct gaatctggat gagttgctat tataatatca atagttttta
    24661 ttcttttttg tctaagataa ctaataatta tattttcgct atcttcatca cctccatcaa
    24721 ttaatatatt cttatttgtg ggtgtttgaa ctaaaatact atcaccttgt ccaacatcaa
    24781 tcatatgtat agatagtaga gattttttgt cacagccgat taaaagtgat attataataa
    24841 gtattgataa aaaaatttta tttctcaata aaaaatcact ccattcatta catgtttata
    24901 taaatttatg atgggtaata taagatatat atgacttatt atattttata ttttaaacaa
    24961 tataaatgaa attaatacca taataaaatt ggagtttttt atatatacaa aataaaattt
    25021 ttaaaattaa gcaataaaaa aggtaggagg attttatgtt tttcaaagat attgaactaa
    25081 attcaaaaaa agagttagac ccatattttg atttagtaga ttatgaagct tgtgagtact
    25141 gttttagtac tttatatatg tggcaacatg tttataagac aggatattat ataggtgaag
    25201 attttgccgt tttagttggg gaatacgaag gagatagctt ctcaatatta ccacttgcta
    25261 aaaaagataa acttccagaa gttgtagatt ttgtccttga atatttttct aaaaataata
    25321 aaaaaatata cttaagaggt attactactg aagtagtaga gtttttaaaa gaaaaatatc
    25381 caggtagatt tgagtatata gaagaaagag atttatttga ctatatttat gatgcagaaa
    25441 gtttaagaac tctagctggt aaaaagaatc aaaagaagag aaaccatata aactatttct
    25501 taaaagagta tgcaggtaga tatgaagcta aattattaga taaagaaaac tttgatgagt
    25561 gtttagtact aatgaaggaa tgggaaagta ataaggaaga aaataatgaa tttgatgaaa
    25621 gtatggatga tgaattaata ggtatcaaaa agatatttaa ccattatgac atattaaaag
    25681 ataaggtaaa agtgtttggt gtttatgtag atggaaaatt agaagctttt agtataggag
    25741 agcttttaaa tccaaatatg gcattgattc atatagaaaa agctaatcct gatataagag
    25801 gtctgtatcc atttataaat caacaatttt tggtaagtga atttaaggac gttgagtttg
    25861 taaatagaga agaagattta ggtattgaag gattaagaaa agctaaactt tcttatcatc
    25921 cttgcagatt tgttgaaaag tacagtgtta gggaggctta atagactata tggagattag
    25981 gtatgctaaa gaagaagaga tagaaagtat aaaagagatt tggagttatt gttttaacga
    26041 tacggagagt tttatgaagt actattttaa tgataaatat aaatctgaaa atactgtagt
    26101 agctttagat gaaggaaaaa ttatttcttc tcttcagcta aatcagtata agttattact
    26161 aaatagcaag gtatataata catcatatgt agtaggagtt tctacacttc cagagggaag
    26221 aggagctgga tatatgaaca aagtaatgaa atttacttta aacgaattgt ataaaaaagg
    26281 gcaattagtt tctatattga tgcctattga ctatagactt tatagaagat ttggatatga
    26341 gcattgttac gaccagattg aatatactat taacacagat gatttaaaaa attttaagtc
    26401 aagtggaaaa atgataaagt ctaatttatc acaaattgat gatttgatac aaatagatag
    26461 aactttttta aatgaggtta atggtaatgt gttaaaagat gaacattatt atgaaaactt
    26521 atttaaagag attcaaagtg aagatggatt tttatacatt catgaaggaa atgaaaaaga
    26581 tggatatata gtttattttc ttcaggaaga taaaatgttt gtcagagagt tattttataa
    26641 aaatatagat gcccttaaat caatgcttaa atttatatat aatcacaata ctcaatgtaa
    26701 gatagttact atatctactc ctacaataga taagataaga tttatattag acaaccctaa
    26761 agatagtgat ataaaaataa aaccttttat gatgggaaga gttataaatg taaagaagtt
    26821 tattgaagat atagatattg agaatgatat taatagctca tttaatttat taatagaaga
    26881 caagtttata gatgaaaata atggtctatt taaaatttct atacagaata agaaggtatc
    26941 tgtagagcag ttagataaaa aaggtgcaga aaagcctcaa gaagattttg atattaaatt
    27001 agatataaat accttaacac agttatcatt ttcttatata gatgtaaatg aagctatttt
    27061 cttaaatgat ataaaagatg tttcagaaga aacactagaa acacttaatt gtattttttc
    27121 taaaaaaaat aactatataa atgaatatat ataggtagag tacgttaaaa gattttaaaa
    27181 atcttttgac gtattttttt gtttagataa caatttatct tgtttaaaaa ttttctattt
    27241 gaaaataatc taaataatat acttttatat gggagtgatt ataattcaaa taagaagacc
    27301 attattaatt atattaatgt ttgtggtagc tatatcattt atcattacaa ataacacaaa
    27361 aaacgacgaa gaacttaatg ataagataat aactatacaa ggagttgtca aaggaaaaat
    27421 tgaaaagaaa agatacaatc agtacaaggt aggggttttt ctaataaatg attatacaaa
    27481 aaataaaaat ttgaagattg gtcagaaagt taatattaca ggtaaattca aaagtttaga
    27541 taaaatgaag tatgatgatt ttgattatgg aaggtatata aaaagtactg gatataaagg
    27601 tatagtgtat ataaataact ataaaattat tgataaaaat aaactttact ctttaatagg
    27661 ggaaataaag ttttatatta gtaaaactta taggtactta tataagaaaa actcagattt
    27721 tataaattct atactattag cagaagttga aaatttgact gaggaacaaa aagaaatttt
    27781 tactagaact ggaactagtc atgtaatatc tatatcaggt cttcatacag gaatattatg
    27841 tgtaattata tcctttctat taagaggtat aaataaattg tataaattat taatactgtc
    27901 gatttttata actttatatt gtataatggt aggtgcgtct ccatccataa ttaggtctat
    27961 agcatttgtt atgatttttt atttatcaat atttattgat agaaagaaag atggaatatc
    28021 ggcattgtct ctaattggga taattttaat tatgaataac ccctatgtta tatataatgt
    28081 aagttttcaa ttgtcttttt tagctacact ttctatatta atattctata ataaaataaa
    28141 tagtattata aaattaagta tggtatcatt gacaatatca tcaaatatat taactttacc
    28201 tataatttat tatacattta aaggtatacc attgctttca attataggaa atttaattat
    28261 agtaccattt gtgggagtaa taatgtattt aagtattgca agtttaatcg tatttaaagt
    28321 aagtgtagta atagctaaaa ttatatcttt ttttaatagt acatttatag aaagcatatt
    28381 ttttttacta gaaaaaatca gcaatttaag ttttgcatac attaatatag aaaatcctaa
    28441 attttatatt gttgttattt attacatagg agtgtttttt tacatatttt acatagaagg
    28501 aaaagagata aaggagcaag agaatgaatt acaaggatat tataaggaat gtaaaagaga
    28561 aaaactttga aaaaatgtat cttttttatg gaagagagta ttatttaatt gagaatgcaa
    28621 taaaagcatt taaagatagt ctaaacgagg gaatgcttga ttttaactta gatataatag
    28681 atgggaagga aatagtctta aatcagctta taagctctat agaaacactt ccatttatgg
    28741 atgatagaaa gatagtaata ataaaagact ttgaactatt aaaaggaaag aaaaagaatt
    28801 ttacagatag tgatgagaaa tacttaatag agcatttaga caatattcca gacaccacaa
    28861 ctatagtttt tgttgtatat ggagatgtag acaaaagaaa atcattagtt aagaaaatag
    28921 gaaacaatgg aattgttttt gattgtgata agctttctga tatggatttg tttaaatgga
    28981 taaaaaaatc ttttgcttta aatgatgtta taatagataa ttctcaaatc atgtatttta
    29041 ttgaacaaga aggatacaga gataagagta gtgaaaagac attatctgat ttagaaaatg
    29101 aaataaataa aataagttca tttgttggta aagggaataa tgtaaccaat gatgtaattg
    29161 ataaattatc tcaaaaaaaa gttgagaatg atatattcaa attaatcgat tatattggag
    29221 aacaaaatgc ttctaatgca atgaaaatat taaatgatat gatacaagaa ggcgaatctg
    29281 tattaggtat attttctatg atagcaagac aatttaaaat aattatgcaa gtaagacagt
    29341 tacaacttga tggatattca acaaaactta tagctgataa attaaaaatg catcaatttg
    29401 ttgtaggaaa agcattaaag caaactaaaa atttttctga tgatataatt gtggaaatat
    29461 taaactatat attagaaagt gattataaaa taaagacagg tcttataaga gatactttag
    29521 cagttgaaat gttggttagt agatactgta aaagagaagc aatttaaaaa atctatatat
    29581 taataaaaaa taaatagcaa gatatgttat agttcagagt agatgtaagc taaattaagt
    29641 ttgatataca atcaactata atatgtctaa ctatttattt tttattgtat aaatttacaa
    29701 aataaaaaac ccctacatag gggttattat atataagaaa tataaactgg taattaagcg
    29761 ttcataccgt ttaatttttg agctaattta gctacttttc tagctgcagc attcttatgt
    29821 atagttcctt tagaagctac ttggtatatt cttttttgag cgtattggaa cttagcaaca
    29881 gcatcctctc tgtttccagc tgcaacagca tcttcgaatc ttcttattgc agtttttatt
    29941 tgagattttc taactctatt taaagcagtt ttcttttcga taacacttat tcttttctta
    30001 gctgatttta tatttgccac cgtgttcacc tccctgtatt tttttatcat attcatcgtc
    30061 aacatattag attttaacag aaataaatct aaaaatcaag gattattatt tatatatttt
    30121 aataatgttt tttattttga ttttttagac tgtaaattgt gtataaataa tttgcaatat
    30181 cttgttttta aattggtata ttttgtgttt taaatatttt gacatatata aattaatatt
    30241 ttatttttgt aacaaatatt ttgagataat acaaactatt aacatacatt ttgatagtat
    30301 ttccaataat tataagataa tacaagaaat tactcatttt tgtaaattta atgaaaaatt
    30361 ataaaaatat aaatttatat gcttaattaa gataattttt ttcattagta tgtatgatag
    30421 atgcatcaaa caatataact aatatgttag aaggttatat gtaattaatg tcagtgttta
    30481 tatagaatag catttgtagc atttattata gttgtaatta ctatttatat catagacttg
    30541 aatagtctaa aaaaatagac tataatattt ttatagacta aagaaagggc tgataagatg
    30601 gacatagcaa aactaatttt aaatcctgca aggcttcgta ttttacaata tattcgactt
    30661 tatggcagtg ttcgtacatc tgatattgta aagtatttaa atgatattcc tcgtgcaaca
    30721 gtttatcacc atgtaaaaat tttagaagag aacaatatga ttgaagtaat taaagaaaat
    30781 cgtgtgcgtg gtactattga aaaggtttat gcactaaagg aatatacaac atctatggaa
    30841 ggagagacct tcgttgcact ttctacagca ttttatgtag gtctgatgca ggaaatgaat
    30901 gaatacttta gcagaaaaga gcaagaccat aaaaaggata atgtattttt ctcttcagca
    30961 ttactatata tgagtgataa tgaatatgaa aatcttctta aaagtatagc tgatttattg
    31021 aaaccatata tagaacaaaa acctaaatca aatctaaaac ttagaaaact ttcaattata
    31081 tcttctccac ctgtagctga taatgataaa acaaaataac atgcaaccca tttttataaa
    31141 aggaggttat atatgaaatt tcatgagttt ggaaataaaa acagaccaca tattatgttg
    31201 attcatgggg gagggaatgc atggtggaac tatttgcgac aagcagaagt tttgtctaac
    31261 aaatatcatg tgatacttcc tacattggat ggtcatggag aagaatatga aacaacatac
    31321 atttctacag aggatacagc agataagttg atatcatata tagatgaaaa ttgcagtgga
    31381 catcttttcg ctttgtgtgg ggtatcactt ggtggtcaaa ttgtcatgga acttttatct
    31441 cgaaaaccta atattactaa aaaagcaatt attgatggta gtatttgcta cccaagacct
    31501 cttatggcaa ggttttgtat tgctggtatt cgattttttg gaggactttt gtttagtgag
    31561 aaggcttgtc gctttcaaat tgctatgatg ccaaaattgt tacctgaaaa tatgcaatat
    31621 ccagataaaa taaaagcgta ttatatgaca gatatgccac atgtaagaaa agaaacattc
    31681 tataatatgt atcgtactta catgatgaac tatacactta aagagaatgt taaaaaaacc
    31741 acagcacaag taatgtactg gtatggagaa aaagaaatga agtgtgtcaa aaaatctgca
    31801 aaactgtttc agtcttatgt gccctcttgt gagatttatg aagctaagag ctataatcat
    31861 gggtatttat cactttatct tccaaatgaa tggttggaaa ttgcagaacc atttttccaa
    31921 aaggaataaa ggtgttaggt gattattgta taattattgc tgttttttta tatagtaaat
    31981 aaattaggta taaatataaa aaattatttt agttaatata ttagtgaaag cttaatagga
    32041 aaattataaa tagattaaag gtagcaccta tataaatagt tgctaccttt ttctgtttgt
    32101 aaataaatga tagataagaa ggtttactga caaatcactg actaaaccca attttcttac
    32161 attgaaagcc ttaattttac ttgttttaaa taaatttaca atttcttgtt ataaaatact
    32221 aaattttgtt aacaattata attagcattt aatttatact ggaggtttat atgattagtg
    32281 taagaacaga tttagcttta gaagctagtg aaatgtgtga aaagtctcaa gaaggaagta
    32341 gtattcctgg tgtaaaaata gaaactaaag aattagaaaa ttgcattgta actaaagttg
    32401 aagtaataga caggcaaggc gcagagataa tgaataaaga tataggaaag tatataactc
    32461 tggaaagtaa tcttatgaag tttgatgatg atgagtcaag agaagaaatg ataagttatt
    32521 taaaagagga gttagtagat atatttggcc aagacaaaaa taaaaaaacc ctggtaattg
    32581 gtcttggaaa tagaaatatt acttctgatg cgttaggacc aaagagtgta tcaaaaacac
    32641 ttgtaactag acatcttttt aaaaattata ataaagacta tgatgatgat ttcactgagg
    32701 tatcagcttt aagcccagga gttatgggtg ttactggaat agaaactagt gaaatagtaa
    32761 aatcattggt tgagaaggtt aagccagata gagtagtagc aatagatgca ttggcttcaa
    32821 gaaaaatgga aagagtaaac tctactatac agatatcaac agcaggaata tcacctggtg
    32881 gaggtgttgg caatactaga aaatctctaa ctaaagaaac tttaggcgtg gatgttattg
    32941 caataggcgt tccaacggtg gttgatgcag caactcttac aatagatgtc ttagatatgg
    33001 caatagacaa tttaatagct caatcagaag aaacagaaag tttttatgaa atgttgaaaa
    33061 aacttaaaga agaagaaaaa tatcatctaa taaaagattc gttagaccca tatgacaaga
    33121 acttaatagt aacaccaaag gatatagatg atacaatcga aaatctatct ataataataa
    33181 gtgaaggctt aaataggtct ttacatccag gtagattggt ataaaataag gaggagcaat
    33241 tatgtttaaa aaatgcatta aagtagtaac cttgactttt atattagtat gcattttacc
    33301 aggaaaatca ttagctttga atcaagatga ttttttaaag tttttagtaa attcatctta
    33361 tccagaagct aaagtcgaag gaaatgatac agaaaataaa aagaataata aaaataaaga
    33421 aacaagtaag gaaaataagg aagaaagtaa agaaggaaac actaaatcta aagatgcaag
    33481 taaagttgat aataaaaaag agagtgaaaa agaatatata aaactttatg ttggtaaaga
    33541 aaatgttcca gatattgagt caaaaaattc agacacaact gaaactaaca caacatctag
    33601 ttcagattat aaggatgatt taagagtaac caaagaaaat ccaagaattt tgatatatca
    33661 tactcatgga tgtgagactt attcaaattc acctgatgga aattatcatt caagagataa
    33721 gaaaaattca gtaatggaag taggaagtgc attaactagt gctttagata gcaaaggatg
    33781 gggtgtagtc catactacta aataccatga ctatccatct tataataact cttatgcaag
    33841 tagcttaaaa acaatacaaa gtatattacc taaatataac tcagtggata tagcgataga
    33901 cttacataga gatgctaggg atttaactaa tccagccact aaggaaaaag accatttaaa
    33961 atatacaact atgataaatg gagagagagt atcaaagttc ttctttgttg taggaggtaa
    34021 aaatacaaat agaaaacaac ttagagcttt agcagaagat ataactgctt ttgctgagaa
    34081 aaagtatcct ggtctagtat ctccaatagt agaaaaagac tatgctagat tcaatcagtt
    34141 tgcagttaag aatcatatgt tagtagaaat agggaataac gcaacaagtg tagaagaatc
    34201 taaggctact acaaagtatt tagccgagat tttggatgaa tattttaaac aaaaaaatta
    34261 gagctttaag ttataattag gagcatgcta aatgagccga ttagaaaaaa atatagaaag
    34321 taaaagtaaa ctaagaaaat acaagtatat tatgagaatc ttatttttga ttactatgat
    34381 tatatgtaca tcagtttgtg tatttatagt ggacagtaat gcaaaaagta tgctaggaat
    34441 gaaacaatat gattttccaa gtattaaatt tgatataaca aaatttgatt ttgacatacc
    34501 aaattttgat atacatgaaa tcataaaaaa atttaataag tagtaataag tgaataaggt
    34561 ttattgatta aaataagcct tattttagtt atatattttt aatgtataaa ataaaaattc
    34621 attaactgta ttttatagga tttactttaa gtttatgata ttatataata tttagtgtta
    34681 gacttgataa tccacatata atgtggataa ttttgtttag tgccaattag tttatattca
    34741 aaaattttaa atttggtata taaagagttt ttagataatg taaaataata actataagaa
    34801 taatcaagag gagggaattt taaggtggac aataaacaaa gtagaactag aaattttagt
    34861 ataattgcac atatagacca tggaaagtct accttagctg atagattaat acaacaaact
    34921 gggcttgtta gtgaaagaga catgaaaagt caattacttg acaatatgga ccttgaaaga
    34981 gaaagaggaa tcactataaa gcttcaaaac ataagattaa tgtataaagc gaaggatgga
    35041 aatgaatatt atttaaatct tatagataca cctggacatg tagactttaa ctatgaggta
    35101 tcaagaagtt tagctgcatg tgaaggagca ctattagtag ttgatgcagc tcaaggtgtt
    35161 gaggcacaaa ctttagccaa tgtttatctt gctatagacc aagatttaga aatattacca
    35221 ataataaata aaatagattt accaagtgca agaccagagg aagtaaaaaa tgaaatagaa
    35281 gatttaatag ggcttgattc aagtgaagct cctcttattt cagctaaaac tggtttaaac
    35341 atagaagatg ttttagaaga tattgtaaaa aatgttcctc cacctaaagg agataatgaa
    35401 gctccattaa aggcattaat attcgattct tactatgatg cttacaaagg tgttgtagct
    35461 tatgttagag tatttgaagg aactgttaaa aagggtatga caataaaaat gatgaatacc
    35521 aataaaaagt ttgaggtaac tgaagttggt gttatggctc caggtcaaac tgaacttagt
    35581 gaattatcag caggtgatgt tggttatatt gctgctagta taaaagatat aagaagttgt
    35641 cgtgttggtg atacaataac agattcaaat aatcctacag aagaaccttt accaggatac
    35701 aaaaaagcta ctccaatggt atattgtggt atatatccag gagaaggaga aaagtatgag
    35761 aatgttagag atgctcttga aaagcttcaa gtaaatgatg ctgcacttga atatgaagct
    35821 gagacttcag cagcactagg atttggtttt agatgtggtt tcttaggtct tttacatatg
    35881 gagataatgc aagaaagact tgagagagaa tttaaccttg atataattac tacagctcca
    35941 tctgttatat atagagttac aaaaatggat ggagaggttg taatgataca aaatcctgca
    36001 aacttaccag aaccatctga gataaagatg atagaagagc ctatagtaaa aggagatata
    36061 atagttccta aagactatgt gggtgttgtt atggagcttt gccaggaaag acgtggaaat
    36121 atgttaaata tggaatatat agatgataga agagtaatgc ttcactatga ccttcctctc
    36181 aatgaagttg tttacgattt ctttgatgct ttaaaatcaa gaactagagg ttatggttct
    36241 cttgattatg aggttaaagg ttatgtagca tctacacttg taaaacttga tatattaatc
    36301 aataaagagc aagtggatgc acttagtttt attgtccatg agacacgtgc tttccctaga
    36361 ggaaaggcaa tgtgtgagaa attaaaagga gaaattccaa gacatcagtt tgctatacca
    36421 atacaagctg ctgttggtaa taaggttatt gcaagagaaa caataagcgc tctaagaaaa
    36481 gatgttcttg ctaaatgtta tggtggagat atatctcgta agaagaaact tcttgaaaaa
    36541 caaaaagaag gtaagaagcg tatgagacaa attggttctg ttgaagttcc tcaaaaagca
    36601 tttatgtctg tgcttaaatt agatgagtaa atccttataa atttagataa agatatttaa
    36661 aagctagagt gattttagaa ttatttctaa attagtctag cttttttatg gtatatttta
    36721 agtaaactca tagagaatat aagtaagaat attaaagaag aataaaaagg tgatatttat
    36781 gattaatata gaaattccta aaaaagtaga ctatataatt aaagaattag aaaagaatgg
    36841 ttatgaagct tatatagttg gaggatgtgt tagagattgt ttattagaaa gagttccaaa
    36901 cgattgggat ataacaacta gtgctagacc agaagtggta gtagaattat ttgaaaaaac
    36961 aattccaact ggtatacaac atgggactgt aacagttatg attgaacatg aaccatttga
    37021 agtgacgact tatagaattg atggaaatta tagtgatggt agacatccag attcaataga
    37081 atttacaaat aacatagtta aggacttgtc aagaagagat tttactatca attcaattgc
    37141 atacaattca aagacaggat tagtcgaccc atttaatggc tatgaagata tacaaaataa
    37201 gtatatacgt tgtgttggaa atccagtaga tagatttgaa gaagatgcac ttagaatgct
    37261 tagagcagtt agattttcag cacaactcaa ttttaaaata gctgaaggta ctaagcaaag
    37321 tattcataaa aaagcagatt tgataaaaaa tgtttctatt gaaagaatcc agacagagtt
    37381 taataaaata ttagtatctg attcatctaa attaaactta ttaaaatcaa caggtttgtt
    37441 gaaatttata attcctgaaa tatgtgaatt agaagatgtt actcaacaca atccatatca
    37501 catctatgat gtacaaaaac atactttaat agctacagaa gtaattgaag atgaactgta
    37561 tttaaaactt acaatgttgt ttcatgattt aggtaaaaaa gtaactaaaa caactgataa
    37621 aaatggtgtt gaccattttt atactcatag tagagaatct gtaaagattg caaagaagat
    37681 tttgaaaaga cttaagtatg ataattatac tattaataaa gttttaatat taatacaata
    37741 tcatgattat agaatagaac ctaaaaggaa gataattaaa aagttattaa ataaacttga
    37801 agatgttgaa ttatttgaag atttaataaa agttaattgg gctgatacac ttgcaaaaaa
    37861 tccaaagtat gcaaaacaaa agatattaaa tcttattgag tgtgaaaaag aatttaaaca
    37921 tataataaat caaaaggagt gctttaatct taaagattta gctattaatg gaaaagattt
    37981 gatatctata ggtataaaac caggtaaaga tataggatat attttaaata agatgcttga
    38041 aatagtcata aataatccag agttaaatga aaaagaaata ctaaaagaaa aagcattaaa
    38101 tatatataca ttttagattc aaatataaaa aatttaatta ctttaaaaaa catagaataa
    38161 aatacttatt tagataaaat aaataatttt tagagaatat tttagaagtt acgattgtat
    38221 gatttttaaa tgcataggca tttactctag gactatagac taatataaaa aaaaactttg
    38281 ggaatactaa aaccataaat ttataaaatt aatattttat atactgtaaa acagtgagga
    38341 gggacagtat gcatactcaa ctacaaaaga ctattggtct ttcagcagct ctttctactg
    38401 ttgtaggtat ggttataggt tcgggagttt tttttaagcc acaagcaata tacactacaa
    38461 ctaatggagc tccaggtcta ggtattatag cgtggctttt aggtggtttt ataacaataa
    38521 ctgctgggct tacagctaca gaaatttcag ctgctatacc aaaaactggt ggtatgatga
    38581 tttatatcga agaaatatat ggagaaaagt taggtttttt aactggatgg atgcaaactg
    38641 tattattttt cccaggtaca tcagcggcat taggagtaat atttgctcaa caagcatctg
    38701 aacttttagg aatgagccca aataatatgg caaatgtact tcctatagca attggagtta
    38761 tactattttt agcgttatta aatataatag gttcatcctt aggaggaaaa gttcagacag
    38821 tagccactat tggaaagatg atacctttaa ttttaataat agtctttgga tttattaaag
    38881 gtcaaagtag tgaagtatta aatccatttg taggagatgg tgtaaatgca tcaaatgcac
    38941 ttggacaagt attaatagct actttgtttg catatgatgg ttggataaat gttggagcta
    39001 tatcaggaga aatgaagtct ccagagaaag atttaccaag agcaatagtt ggtggtttat
    39061 cattagttat ggcagtttat attataataa atatagctta tctttgggta gtaccagcaa
    39121 gtgaattagc aacagttact tccccagcta cattagtagc aacaagattg tttggaaata
    39181 taggtggaaa agtaataact gtgggtatat taatatcagt atttgggaca ttgaatggat
    39241 atttattaac aggttcaagg ataccttata cattagctga aatgggaact ttaccagcat
    39301 ctaaaactct tttaaaagta aattctggag gttctcctgt aaattcaata ctacttataa
    39361 ctgtgttagc ctgtgtatat gcattatctg gtcaatttaa tcttttgaca gacttaacaa
    39421 tattttcaat atgggtattt tatgtattga cttttatagg tgttatgagg cttagaagac
    39481 aaaagccaga tttacacaga ccatataaag tccctttata tccaataata ccaattatag
    39541 ctatattagg tggactgttt gtaataataa atcaaatatt gacttcaaca gtcatatctt
    39601 taggcggaat atttataaca ctattaggtt taccagttta ttattatatg aaaaaaagat
    39661 aactacttaa tttaagttag ataaatttgg gtaaaggtga aaattaactt ttatccaaat
    39721 tttttatact gttatacaat ataagtatta tatattgctt gatgtgctat tttgtttgat
    39781 ttataattta gtaattgatg ttttaagata atttaagaaa tgaggagtga aagatgctag
    39841 gactttatgt acatattcca ttttgtgtga aaaaatgtaa gtattgtgat tttaattctt
    39901 ataaaatgga catagattca aaaaaaagat atatagaaga cttaaaaata gaaatggaat
    39961 tatatagtaa taaattatat aaagataata aatataaaaa taaagaatgc tgtagtctaa
    40021 ataaaaatga caaaataacg agtatatttg tagggggagg tacacctagt atactaacct
    40081 ctgatgaaat aagagaagta tttataagta taaaagaaat gtttgatata gatgagaatg
    40141 cagaaataac tatagagtgt aatcctggga cattaacatt agaaaaatta aaaactatga
    40201 aagagattgg cataaataga cttagtatag ggctacaagc tattcaagaa aagcatttga
    40261 actttatagg aagaatacat acttatgaag aatttgagaa aaactataaa gatgcattaa
    40321 gtgtaggttt taaaaatatt aatatagatt taatgtacag tcttccaaat caaactctat
    40381 gtgattggaa ggaaactctt gaaaaagttg tacacttaaa tccaacacat atatcagctt
    40441 attcccttat actagaagaa ggaactgaac tatacaatat gtatgaaagt aataaatttg
    40501 aacttataga tgaaaatgta gatatagaaa tgtatgagta tacaataaac tatttaaagt
    40561 caaaaggata taatcaatat gaaatatcaa actactcaaa agaaggctat aattgtgagc
    40621 ataatatttt atattgggaa tgtgaacatt atatagggat tggagcagga gcttctggct
    40681 atattaatga gaacagatac aataatgtag aatctttaga agattatcat ttaagtttgg
    40741 taaaaagaga aaaaccaata caagaaaatg aaattctttc tgagaaagat atgatagaag
    40801 aaaagatatt tatggggctt agaatgaata aaggcataaa atttgaagat tttaaaaaaa
    40861 aatttggaat agattttaga gagaagtata ataagcaaat agaaatgcta ttagctagaa
    40921 agcttataaa tcagagcttt gaaggtatac aattaactca aaaaggaaga gaaatatcta
    40981 atagtgtatt tatagaattt atggaataat ttttaaggtg ttgacaaaat aaatttataa
    41041 tggtatataa tatatataat ctttttagca ctcgaactta gtgagtgcta acagctagga
    41101 ggtataataa tatggaatta aatgaaagaa aattaaatat cctcaaagct atagttaaag
    41161 attatataga aacagctgaa gctataggtt ctagaacaat atctaaaaga catgatttag
    41221 gtgttagtgc tgctaccata agaaatgaaa tggctgacct tgaagaatta gggtatttaa
    41281 ttcagcctca tacttctgcg ggtagagttc catctgaaaa aggctataag ttatatgtaa
    41341 actctcttat gagtaaaagc gaattggatg ataatgataa gattcttatt gaacaatgta
    41401 tgaatcataa tataaatcat ataaaagaat tgattcatga aacttctaag ctattatcgc
    41461 aactaacaaa ctatacaact gtagcagtta ctaagagttt aataaatcaa agtgttataa
    41521 agcacataca gttggttgct atgaatgaca ataatatttt acttatagtt gtaacagata
    41581 aaggggactt aaaaaaagct aaccttacaa ctaatgtata tttagatcaa tctaagttaa
    41641 atttgatttc tgataatctt acaaggaagc tcttaggtaa aagtataaca gatttggatg
    41701 ataatctaat tgcttttatt aaatacgaaa taagtgagta ttctgggctt atagatgagc
    41761 ttttaaatgc tttgaactca aatatgaaag aagaagattt ttctctatca ttaaatggag
    41821 ctactaacat atttagttat ccagaattta atgatgtatt aaaagcaaag tcatttttaa
    41881 atatgttgga aaaaaaggaa acaatagctg acataattaa atcaaaggga atacaaaaag
    41941 acaatctaaa tataataatt ggtagtgata atgattgtga actggcacag gattgtagta
    42001 tagttacagc aacttataat gtagatagag acttggttgg gcgaataagc tttataggcc
    42061 ctaccagaat ggattatgct agaatttact ctataataaa ttatatgagt ttattaatta
    42121 atagaaagta aagaaatttt aaattgcgag gtgtaaagtc ttggataaaa aaaatgagca
    42181 acaggaagta agagaagaaa atgatacttc tattaaccaa gaatcggaaa ctcaagtaga
    42241 gttagaagag gaagttgtta atgaagaatg tgaaacatca agtgaaaaaa ctgatgaaaa
    42301 agaagttgat gatgaaaatg taacagatat aaattctaag ttagcagaaa aaaaattgca
    42361 ggatgagttg gatgaattaa atgataaata tcaaagactt caagccgaat atgcaaacta
    42421 cagaagaaga actcaacaag agaaagagac aataggcgta tttgcaaatg aaaaaataat
    42481 aactgaattg ataccagtta tagatagtat ggaaagagcc ttagatgcat gtgaagataa
    42541 ggaagatact atgtataagg gtataagcct tgtacataaa caattaatag acactttagt
    42601 aaaatttgga gtagaagaaa tagaagcaga atctaaggaa tttgacccta atctacatct
    42661 agcagttatg caagaaagtg tagatggagt tgaagcaaat caaatagtaa tggtacttca
    42721 aaaaggatat aagctaggaa ctaaagttgt aagaccttca atggttaaag tttcttgcta
    42781 agtataaatt aaaggttaaa ttatttaacc taatataaaa tattaaaata agtaaaactt
    42841 aattatataa ttactcgagg aggattttag tcatgggaaa aataatagga atagatttag
    42901 gaacaacaaa ttcatgtgtt gcagtgttag aaggtggaga agcacaaata atagcaaata
    42961 gtgaaggtat gagaactact ccatcagtag tagcatttac aaaagatggt gaaagaatag
    43021 ttggagaacc tgcaaaaaga caagcagtta caaatgcaga taaaacaata acttctataa
    43081 aaactcatat gggaactgat tataaagtaa atatagatgg aaaatcatat actccacaag
    43141 aaatatcagc aataatatta caaaaattaa aatcagatgc agaaagttac ttaggacaaa
    43201 cagtaacaga agcagttata acagtaccag cttactttac agatgctcaa agacaagcaa
    43261 ctaaagatgc tggtagaata gcagggcttg atgttaagag aataataaat gagccaacag
    43321 cagcagctct tgcttatggt atggataaat tagaccaaga aaagaaaata ttagtatttg
    43381 acttaggtgg aggaactttt gacgtatcta tacttgaaat aggagatgga acttttgaag
    43441 ttttagctac agctggtaac aatagattag gtggagatga ctttgaccaa atagtaatag
    43501 attatttagc agaggaattt aaaaaagctg aaggtgtaga tttaagaaat gataaaatgg
    43561 ctcttcaaag attaaaagaa gcagcagaaa aggctaagaa agaattatca tcaacaatga
    43621 gttcaaacat aaacttacct ttcataactg ctacagcaga aggaccaaaa cacttaaata
    43681 tagatttatc aagagctaaa tttgaagaat taactagagg tttagttgaa aaaactatgg
    43741 aaccaactaa gacagcttta caagatgcag gactttctac aggtgatata gatgatgtat
    43801 tattagtagg tggttctaca agaataccag cagtacaaga agctgttaag aagtttatag
    43861 gaaaagaacc tcataaaggt ataaacccag atgagtgtgt tgcagcaggt gcttctatac
    43921 aagctggagt tttagcaggg gatgttaaag acttattatt actagatgtt actccattat
    43981 ctcttggtat agaaactatg ggtaatgtaa tgactaagat aatagaaaga aatactacaa
    44041 taccaactaa gaagtctcaa atattctcaa ctgctgctga taaccaaaca gctgttgata
    44101 tacatgttct tcaaggtgaa agaagtatgg cttatgacaa tacaacttta ggaagattcc
    44161 aattaacaga tataccacca gcacaaagag gaatacctca aatagaagtt acttttgata
    44221 tagatgcaaa tggtatagtt aatgtttcag ctaaagattt aggtacagga aaagagcaaa
    44281 aaataactat aacatcaaac actaatttat cagaagcaga aatagagcaa aagataaaag
    44341 aagcagaaat gaatgcagaa gctgacaaac aaaagaaaga aaaaatagaa gcattcaatc
    44401 aagctgaatc tactatatat caaactgaaa aaactttaaa tgaacttgga gataaaataa
    44461 gttcaggtga aaaagaggat atagaaaaag ctatagcaga cttaaaagct gtaaaagata
    44521 atcaagatgc tacagcagaa gaacttaaga aagctacaga tgaagtaatg actaagttcc
    44581 aaaaagtatc tcaagaaatg taccaaaaag cagctcaaga acaacaagcg gcacaaggtg
    44641 cagaacaagc acaagacaat ggacctaaag atgacaatgt agtagatgct gactttaaag
    44701 aagtagatga agataaataa gctatataat atgtaacact aaatgataat tggaacatat
    44761 atgatttgtg aaaatcatat agatgttaaa gatatatgga caatatacat cataaagaat
    44821 ataatatatc atagattata taacttaaag tagaatataa gaattaatga tagcttgtag
    44881 tttattattc tacaagctat cgttttgtaa aatgaaggtg gtgtcaattt ttgagtacaa
    44941 aaagggatta ttatgaagtc ttaggtatca gtaaaggtgc agaagctcaa gagataaaaa
    45001 aagcttatag aaaattagca atgaagtacc atccagatag aaatcctgga gataaagagg
    45061 cggaagaaaa atttaaagag ataaatgaag cttatgaagt tctatctgat gatacaaaga
    45121 gaaaaactta tgaccagttt ggacatgatg ggctaaatgg tcaaggcggt tttggtggtc
    45181 aaggaggatt tggcggtcaa ggctttggtg gttttgaaga tatgttcgga gatatatttg
    45241 gtgacatgtt tggaggaagc ttcggaggtg gaagagcaag aagaagaggg cctcaacgtg
    45301 gtgcagatat aagacagagt gttactatat cttttgaaga agctgcattt ggtaaaaaga
    45361 tgtctataaa agtaaataga agtgaagaat gtgaagaatg taatggaact ggagcaaaac
    45421 caggaacatc aaagaaaact tgttcaactt gtaatggaac tggtcaagtt agaactgttc
    45481 aaagaactcc atttggtaat atagcaagtt caagaccatg tagtgcttgt aatggaactg
    45541 gagaagtgat tgaatctcca tgtagtaaat gtcatggaac tggaaataca agaaaagtta
    45601 agactataga agtagatata cctgctggta tagatgatgg tcaaatgata aaactttctg
    45661 gtcaaggtga agtaggagaa aaaggtgctc caagaggaga cttatatatt gtagttaatg
    45721 ttaagtcaca tccactattt actagagatg gaaatgatat ttattttgaa atgccaatta
    45781 catttgttca agcaacttta ggtgatgaga tagaagtacc tactttggat ggaaaagtta
    45841 aatatagtgt gccagagggg acacaaactg gaactgtatt tagacttaaa gagaaaggaa
    45901 tccctagaat tagaggtaat tcaagaggag accaatatgt taaggttgta gttgaaattc
    45961 ctaaaaaatt aaacgataag caaaaagaac tattgagaga atttgcgaag gagtgtgggt
    46021 cgaacgtcca cgaaaagaag aaaaccttcg gacaaaagat agaagatatg tttaagaaaa
    46081 agtagttaaa gtagccaata tataatcata tttttaaaat cttagttatt tataagattg
    46141 tcaaaggaga tattatgatt tttatattgg ctatttttgt ataaaaaata aatgtgcata
    46201 aaataaaact gaatataaat aattttctga aaaatacttg tcctaaaata tataatatgc
    46261 tataatataa tcaaataaaa gaaaggggtg attccagtgg gaacggatgt ggcactgcca
    46321 aatgatgaag aaaactttat tgtaatatac aaaataaaat tagatacttt tagtgctaga
    46381 ttaagtactg gaaaaactca tgagctggga taattattgt aattacctag actcaaactt
    46441 ttagtagtaa aatagtaata ttatatagac aaggtatcta atttttaaat cttttgtatg
    46501 aattaaaatt tttataaagg gtatatctga ttagaatgta attagggaaa cctagttgtg
    46561 aattactttt agtatataca tgtgtaaaga aatatgtata tggtattggt tattgtgcta
    46621 gatgtgtatg taaaggaata tgtgtctagt tgggtatatg taaagaaata tacttataat
    46681 ctatgaaaaa tttttagtat aaattgctaa aacattgaga ttgtttttta tagaaagtta
    46741 gtttatacca actaaaaatg tgcttaagat ttgtttgatg tttaattata aataaggttg
    46801 tagataaaaa aataagtaaa gacaaaggtt taaaatactt gctttaaata tagtttgttt
    46861 tgaacacttg ccaaattaaa ataattaata aaatagacat cttctcatag taaaatatac
    46921 tgtgagagga tgtttttgct ttgttcataa acttttatga aaaatttgca tagtgtcaca
    46981 ttttaatata ctatatatat aataaaatat agataaaatt aaatgattca aaagagaaag
    47041 gtgaaaagag atgtattata taggtgtaga cataggagga acaggtatac aagcaggagt
    47101 agttgataac tatgggaaaa taatttttag gtcagagtgt aaaacagtaa ttgaaaaagg
    47161 ctttgaagga atacttaatg acataaaaat aatgatttat aagttgttgg aagataataa
    47221 acttactatg agtgacataa aatctattgg atttggtgtt ccaggtttta taaataaaga
    47281 aggattagta acttgtgtaa atttaaaatg gaataaaaaa gcttttaata aagagttaaa
    47341 aagaagattt ccagatgttg aaattcatgg agaaaatgat gcaacagtag cagcacttgg
    47401 agaagcaaag tttggaagta tgaaaggtgc taatgttgga gtattatata ctttaggaac
    47461 tggagtaggt ggaggaatag taattaatca aaaagtcttt tctggagcac atggattggg
    47521 ttcagaaata ggtcatcaaa ttataggaga aaactatttt aattgtaatt gtggaaataa
    47581 tggttgtgta gaaacttttt gttcagcaac tgcaattata aagtattctc aaaaactgat
    47641 agaagaagga gaaaagagta ggattttaga tttggcagag ggaaatttag aaaatgtaaa
    47701 tgcaaagatg gtgtttgatg catatagaga aaatgattta gtggcaatta aagtaataaa
    47761 tagatttaaa gagtatttag caaaaacttt tgcaaacaca ataaattctt tagacccaga
    47821 aattatatct ataggaggag gcatttctaa atctagcgat ataatacttg atggaataga
    47881 agatttggta agaaaatttg tattatacaa aacagaagat atagctacaa ttacatgtgc
    47941 aactttaggt tcagacgcag gaattatagg agcagctttt ttataatgta aatgaactgc
    48001 ttattggagg cgtgatgata tatgggtaaa acaaatgcta gaagaacgct ttttttgata
    48061 gcaataggtt caggaactat gctgaatcct cttaattctt ctatgatttc acttgcttta
    48121 catagtatac aaaatgattt tcatattagt tttacaactg tatcttggat tgtttcagca
    48181 ttttacctag ctagtgctat tgcacaacca gtttcaggta agataggaga tttaataggt
    48241 agaaaggtct tatttttgag tggtttgatg cttgtaattt tatcagcatc catagtacca
    48301 cttgttcaat cattctttat attgattttt ataagagtaa ttcaagcaat tggaacaagt
    48361 accctatatc catctggagt tgctcttata caaaataata taaaagaaag acaatcttct
    48421 gcattagcag ttctaaccat ttttgcttca actacagcag gattaggtcc gacacttgga
    48481 ggattattgt tggacttagg aggatggcat gctatatttc ttgtaaatat acctgttgtt
    48541 ttggtttcta tatgcttagg gtatttttta ttcccaaaag aagtaaaaga aaagaaatct
    48601 ataaaagaaa ctttaaaaaa tgttttgtca agattagata ttattggcat acttctattt
    48661 tcaattgcaa tgatttttat attactattt ttgctatcta tgaaggagtc ttttaacttg
    48721 gaacaacttg tatttggtat aatactgatg tgtcttttta tttggcatga attgagaaca
    48781 aaattgccat ttatagatat taagcttttt gtaagtaatc ctaaattgtc aaaagtttac
    48841 ttgcaattta ttatattaaa tttctttaat tatattttgt tttttggtct tcctagttat
    48901 tttcaagatg ccttacatta tagtgcaaag tcgactggat tatttatgct atttatgtct
    48961 ggtataggta tatttatatc tccattaact ggaaaatgga tagataaatc tggaactaga
    49021 tttcctcttg ttacatcatc aatctttatg tttattagtg cagtacttct ttcagtagtt
    49081 tttatccata tacctgtaat aggaaagggt attatattat cattagcagg tattagttat
    49141 ggagttggta atgtagccct gcaatcctct atgtttgaag aaagtcctcg tgacagtata
    49201 gggacagctt ctggtctgtt tcaaactagt cgttatttag gttctatttt agcaactgtt
    49261 ttacttggaa tggtatttga agtaaatata acatcagaac aatttcagat attaggttat
    49321 gttatggtgg tcttaggtac aataagtttt ttattaaatt ttatattaaa gaaagaatta
    49381 agaaaagtag aatagtggat ttatggattt aataagtcta ataactgata agtttaaaaa
    49441 tattgtttgt aaaacataac tggtttgata tttattaatt ctaagttttg aagcatagag
    49501 tactcgataa gtatacaata ttttgaatta aaattaagca gaaatttgaa ctagtctttc
    49561 aaatctctgc ttaatttagt agttaaattt ttttatagag aaaataagat ttgacactta
    49621 gataaaaaat cattgcattt tcaaaattct ttaaatcaaa cccaagtatc tcattaatct
    49681 tttccaatct atatactagc gtgtttctat gtaaaaacat actagtagat gtcttagaaa
    49741 tatttaaatc attatttata aattttattc ctgtatatag aagttcttcg tcaatagatt
    49801 taatcttatt aaaatctatt aatttattga gtatattatc ctcattaatt aaatctaaag
    49861 ttttatatct aataaagtca aacaaattta aaaacttatt ttcagcgttg tcttttataa
    49921 tatgaggtag taaatcagaa cataactgta atctaaaatc aaaatcagct ttactatcta
    49981 attcaccaag ataaaaaata taagtacttt gataagtttc agtttcaatt atattttgtg
    50041 tagttacatt aatatctaag ttatatttag aatttactat aaaaaaacca ttgtgttgta
    50101 ttatatttat atcttcaaat aaattataaa aaatattttt tagatattca aagtctatat
    50161 gagattggta aattaaataa aagttgcctt ttaaattatc gagattattt ttatctagaa
    50221 aatcaataac ataaatagca ttattgaaag taactaaatg gctactagca tctaactttt
    50281 ggttttcata tggatagata tttatttttt tattaagctg tctttctaaa aaatctttta
    50341 actcttccat aaagtctcct taaaaataag attatattct tatcttatag ctaaagtagt
    50401 ttctttgtca aatatgtgta ctctactatt gtcaactgaa agtgttaagt catcaccaac
    50461 attaattctg tttttactgt caaatctaat tgttatcgta ttttcgccta gttttaagtg
    50521 agcataagtt tctgctccca taagttcttt tatttcaatt cttgatttaa aacaagtatc
    50581 tggattagat ttaataaaag tatcttctac atgaatatct tctggtctga tacctataac
    50641 aacttcatta ttaatataat tgtcttttat taaatcatag tgtttttctt tattcaattt
    50701 aagcttaata tgttcatttt gtgcataaat ttcatcattt tcttctatta atattacatc
    50761 aataaaattc atctgtggtg ctccaataaa tcctgcaaca aatatattag ctggataatc
    50821 gtatacatct tgtggagttg caatctgctg aactactcca tccttcatta ctacgattct
    50881 atcagccata gtcatagctt caacttggtc gtgtgttaca tagataaatg tagttccaag
    50941 ctgttggtgt agcttagtta tctctgttct catagcagtt ctaagttttg catctaagtt
    51001 ggatagaggt tcatccatta agaatacctt aggatttcta actattgcac gtcctaaggc
    51061 aaccctttgt ctttgtcctc cagataatgt tttaggtttt ttatttaaaa gaggtagtaa
    51121 gtctaatatt ttagcagctt ctttaacttt tttgtctatt tcatctttag gcaaatttct
    51181 aagtttaagt gcaaatgcca tgttttcgta aacagataag tggggatata aagcatagtt
    51241 ttgaaaaacc atagctatat ctctatcttt tggttctata ttattgacaa gtttatctcc
    51301 aatgtatagt tctccttcag agatttcttc aagaccagca atcattctaa gtgtagtcga
    51361 tttaccacaa ccagatggtc ctactaaaac gataaattct ttatctttaa tgtctatatt
    51421 tatatttttt actgcgttaa atccatttga gtaaagtttt gatatatttt ttaaaattac
    51481 ttctgacata atatcctcct tgatatttaa attttaatta tatagtatca aattttttta
    51541 ttatattaaa tgtgtaaaat gactaaatgt atttgtttaa tatgtatatt tagtttttag
    51601 caaagaataa aactaacagt agaatatatt taaaaaatta aaaaacaaaa ttaaatttat
    51661 tatcaaaaaa tcattttatg atatgtttta tttttatatt acaaatagag aataatgaat
    51721 tatactagaa agtaggaggt gaaaatatga gcagaatttc aatttttatg gaatcatgta
    51781 atggcaaatt tccaaatgaa aattcaatgt tgtcagtttc ctttataaaa aatattttaa
    51841 atgcagaaaa caaagatttt gctaaaaaca tatttaacta tggggaagaa agaaaaaaca
    51901 ataagcaaat aaaggatata aatacagcca tttacatacc aaatcttata agaagcaaaa
    51961 atgagttact tgttgatgga gatataatat tcaatataag tttttataat tattctatgt
    52021 tcagtaaatt atacaatgga attttaaaag taaaggagct agaatataaa ggttatagat
    52081 ttaaaataaa aaatataaaa atacataaag aacacgagat aaaagaaaat ggtgttatat
    52141 ttaaaactat gtctcctata atagtaaaga ataaagaagg taaatattta gatgtagaag
    52201 attcaaatta tatagaatgt ttaaactata tagctaatct aactctggag agtataagag
    52261 gtagtgggct tagaaagcca ttggaattta tacctattaa cttcaaaaaa agagtgttaa
    52321 aagaaaagat tcgtggattt aaagtgagag aatattacta tataaatgct tatgcaggaa
    52381 catttttttt aaaaggagat atggaagatt taaatgcact gtataaaatg ggaattggat
    52441 atagaaggac agaaaatgca ggaatggttg atatattaaa gtagcgaggt gagagaaata
    52501 aaagctaaat tttatagatc aggtgatatt gtaagagata ttggcattgt ttacttttat
    52561 gaattactaa tggatttaaa aaacgaacta gaaaaaaatg attataagct aaaatttact
    52621 tgtgagttaa atagaaatta tttatcttta gaatctgcag aatatataga ccctaagtat
    52681 atatctgatt atatattaga aaatcgactt tttaaggtat ttaaaaatgg gaaaaaggaa
    52741 accttagaaa aaatatttag taatatagat aatttaacgt atgtaaattt tattgaagaa
    52801 ttagataaat cttctgcaac tgctaaacaa aaggaagata ttaaaaaaga ttttaaaaac
    52861 aggaggtttc catatgttag aaatagtggt aagtatggat taaacacgag tttagaaaat
    52921 atggaaacaa atgtaaaaag aatagtagat actgtaatta aatcaaatat agagatagat
    52981 gaaatagata aacttgattt gacacaatat gaagaaaaag atagtgtatg taatgtttgc
    53041 aatataaata aaacaacaaa actagatatt gatttaagag aacgaaagga ctctaagtat
    53101 aactttttat ttagaggtgc agaaaaatca ggctttaaaa gaagtggaca agttgaaagt
    53161 aatatttgtt ttgagtgtga atttttcaac ttaatgtgtc ttttatatat aaatttaaaa
    53221 agaccaatgg tttttgcata tacgaatgat ttaagagaat tagcattttt aaatcataag
    53281 ataatgctta aaagaaaaat gtattcagat aaatcatttt ataaaaaact tttgcatgaa
    53341 aaaataagtt caattagact atatagcttt gatattgata cgaataaagg tataattcta
    53401 aagtttgatt ctattataga gtataaagaa ttattaaaaa aaattgaact tatagatatt
    53461 atagataatt ataatttttc aagagagcca ggtaatacta gaaatttggg taaaagaatg
    53521 attgataatg ggaatttatc aaatttaaaa gaattattac ttgataatat aagtgtttta
    53581 aagcaatcaa ctggaacttc tcgagaaatg gattttgtat ctagctcata taatatcggt
    53641 ttatatataa aattgtgttg gattgtagat ggaggggagg agtataaaat gaaaaactat
    53701 atggaaagta atttaatata ttcaagagta ggtaaagact tatttaataa aatgacagat
    53761 gaatccagaa gaaaaaattt ttctatgaaa gtgattcaac tacttaagtc taatgataga
    53821 actcagctat ttcaaactat aatgcatgtg ttagtttcta atggaatctt aataggagaa
    53881 gggtttgtag aaggtctaat gcagagttct gaattagaat taaatacgaa tgtaggtcta
    53941 tttattcaag aaatcatgaa ataaaaaagg gggaaaattt aaaatgaatg aaaaaagagt
    54001 aaaaggagta acattaacta tactgacaga aagcccagtt ccactttcat atgaccaagg
    54061 gtatggaaat tatacaccaa taaaaaaaga acaatataga gaaaaaatac atgcaaaaac
    54121 tagtatagcc acaataacat atgatttaag aagaatgctc caccaagaat atggttggaa
    54181 tttaagtaat attgtttttg gaaaaaaagg aaatatatat ccatctatta agaaaaatgt
    54241 aggatgtgta gatgaaaatg gattagagac tgatgtattt ggttatttaa taccacttaa
    54301 agataaaggt tctataagta aagcttctcc acttagaata atacctttta ggtcattaaa
    54361 tccatataaa ggttcaacac agctaataac aaatagaggc tttttaagta gtgaatttgg
    54421 tagaaaatat tatgatgaga aggaagaaaa tgaagtacct agagatgaaa actttccaac
    54481 atcacaagca ttagcaatag aagagacttt atcagattac tacgtttata caataacatt
    54541 ggagcttgat agaataggag tagtagaagt agaagatgga aagttacttt taccagaaga
    54601 aagaaaattt atgagtaagg agttaagaga aaaagcagta aaagatatat tagatgcaat
    54661 tactgtgttt acaagaaaca taaaacattc atcagtacta ttaaaaccac ttgcagtaat
    54721 aggtggagct tttgataaag ttgtaccatt tttttgggat gatgtagatt ataatgctga
    54781 tagtggtgag ataaatttag agggagtcat agaaactata gaaagttata gccttaaaga
    54841 aagtaacaca atacttgcta tcaatgatag attaaaaatt tctaataaaa aggaattaaa
    54901 taagtttaat ttaagtaaat accctgtaaa agagattaaa aatctagcag atagattgga
    54961 aattggagaa gataatatgt ggtatttaaa agagtaggag gttttttgaa ttatgaaagt
    55021 attacaatgt aaacttaagc aaccaactgc tcactataga gacccaaaag tatttcaaaa
    55081 tgaatatata agtactttaa atttaccatc taagaccaca ataatgggaa tgataacgta
    55141 tttatgtgat agaagattaa actctgatat agatatagga ataattggaa cacatcatca
    55201 tagagaacta gaattttcaa gaggagaaaa cattgatttt tggaatgaat atataaaaat
    55261 gggaaaaggt aaagataaag aaaagttttt gcttaaagga aactactatg attactataa
    55321 agagcataaa gctcaaaata gcatattaaa ctatgaagtt cttaaagaag tagaactgac
    55381 tatatttatt tcttgcaagg atgatgaaga acttgaattt attagaaaaa agcttgaaag
    55441 tccatgtaaa tatgcaaatt taggaagaaa agaagatttt gtaataccaa gtgaaaaagg
    55501 gtgttttgtg aaagaagtta agttaaaaga ggttatacct atgaatactc gtgatgctat
    55561 aaaagaaaat ataaaattaa agaatacata tgtaagagtt gacttaagag ataaagagaa
    55621 tgtcgagact ataataaatc aaggtgtatt gattgcattg cctcataaat ataaagattt
    55681 agaagcaaat agagatgata gagtttatga gttttgccat tatatatatg ttgataatga
    55741 tggtatatat cctaaaaata ttaaggtaaa tgtatgtatt gatacaaaag aagtttttac
    55801 atggctttaa attattttga aagataggtg tttatcatga aaaatatcat gcaaaaagaa
    55861 atattagcta agtcatacaa tttaagaact aatcaacaag aacaaacatt agttgaacat
    55921 attaaggatt tatttgaagt attagaatct atattagaat taaatctata ttcagataaa
    55981 gatgtagaga tactaaaaat atgttgtgct ttgcacgatt taggaaaaat taattcaatt
    56041 atgcaacaaa aaatggagat taataataaa atatcgtata gttgctcaga agaagagagg
    56101 aaaaagttag agtctgacaa aaaatcttta aaaaagatag caagacataa tatattttct
    56161 ggagcatttt taaaagatat attagaaaaa atgaacttgt cagaagaaga taagttatat
    56221 atatataaaa gtataatgtt acatcatgga aactatgaag attatatgag gttaagtatt
    56281 agtaaagttc aagaagaaat ttatgagtat attgaaaaag gcattttaga aaaagaagag
    56341 tttaatctga aagatataga aagttatata aatagcattt taaatgtaga ctttaaattt
    56401 ggtgaagatg tattggatta tgattacata gataaattaa gtgaaagtat gtttatagac
    56461 agtgattata acaatggtca aatagatgat tcagttttaa attatagaaa gtttaagtat
    56521 attttatata aagggatgct gaacttaata gaccatagtg catcaagtag acagaaaggt
    56581 ataaaatttt ataacgattt tactgatgaa gaaattgata atatgatttt aaatgagata
    56641 tataagagtc agggtaattt ggaaaaaaat aatatagagt tcaatactat acaaaaaagg
    56701 ttgagagaac tttctggaag aaatgttctg actgttgcat ttacaggttc aggaaaaaca
    56761 gctgcagatt atagaaagac ttttaaaaga aaattctttt tagtgccaaa taaaatatca
    56821 gcagaaagtt tttatagaaa aaatattttt caaaataaaa atgatttaga tacaagaagt
    56881 agtaatgatt acattggatt acttcatgga gatataaact tatattcaga gaatgaagat
    56941 aatggagaac atgattttgt attgacatta agggatatag atttgagtat aaacttttgc
    57001 aaaccttgtg taattgcaac agttgatcag ttattactta gtatgtttaa atttccggga
    57061 tatgaaaaaa tattcgcagc cgtaaaaaat gcatctataa ctgttgatga agtacattta
    57121 ttggaaccaa agatgttttt gattttgata tattttatgc agtttgcatg caagtactta
    57181 gatgttgatt ttcacttaat gacagctaca cttccaaaat catataaaga gcaaatgata
    57241 aacaagggaa ttatttttca agaagaaagt aatgaaaatg ttactgataa aggagaaata
    57301 gtttttattg agagcaacaa ggaagaagag atttgtgaag gtaaagatgt aaaggtttct
    57361 tttataaagg aaaaagaaat caagtcaata gttgaagatg ctttagaaaa taaacaaaaa
    57421 attcttataa taaagaatac tattgattca gtcaatagaa catatgagtt tttaaaagaa
    57481 aatttatctg ataagtatgg tggtgtagat atagatgttt tacatagtag atttaagttc
    57541 aaagataaaa aagagaaata tagtaaaata ttaaatggag agggtgatat ttggatatca
    57601 actcaatcag tagaaatatc cttggatttg gatttcaata taataataag tgatttagca
    57661 actatggata gcttaataca aagaatgggt agatgcaata gaaataataa gtatgaatat
    57721 ggaaactttt atattctacc tagtgaagat aaaatttatg acaacaaact taagagcaca
    57781 actaaaaaca tcttaaagga catattaaaa actaactcta tttttacaat gggaattaga
    57841 aaaactattt tggatgatta ctatgataat agtgtagtca aaaaatattt tgaagagaat
    57901 tttataagtt gtgataaaga aattaagaat atttatggtg taaataaaga aatttttgat
    57961 ggattggatt taatttttaa ttttgaacct tacaaaaata ttgttgatag taagaaggaa
    58021 gcagctaaaa tttttagaga tgttgatgtt agttacaaaa ttattctgga agaagatttt
    58081 tataaggaag atagagagtt acaacaagat tcgattcaag tatcagggtt tatatttaat
    58141 agattatatt attatgggtt aataagcaaa gtagaaggat acatggtact aaaatctagt
    58201 agtaagtttg agtataatag tactgtaggc ttaattttga agtgagcata tgtttaaata
    58261 taatatacct ttagacattg aaaatacaaa aagtactact gtaagctcac tgtaaaaact
    58321 attttttaaa aggaaaaata agttgtgaga tagagtgttt acaagtgtat atagataata
    58381 aatttgaggt ttaagattaa ctaagtgggt ttcaggtaca tcgcgaaaat aattataagt
    58441 tggagatttt gtatgtttaa gattaactaa gtggatttca aatattgata tagtagatat
    58501 aatagaagtt ttattattag gtttaaaatt aactaatcgg atttttaaat attctgacta
    58561 ttatataagg atattattag attgaattta aaaccaataa tataaaacaa caaaatatta
    58621 tatttatgat ataataaaag ttatggaaaa acaggaatat tactatagag aagagaggta
    58681 aacttatgaa taattggata gaagtaacta taaaaacaac aacagaagca gttgaaccta
    58741 taacgaatat actatatgaa caaggagctg gaggagctgt aatagaagac ccaaaagact
    58801 ttttattcca aaagaaaaat gaactagact gggattatgt tgaagaagaa gtatttaaaa
    58861 agaatgaaga agatgatgtt cttataaaaa cttatgtttc tgaagaaaaa aatgtgatgg
    58921 aatttgtcga aataataaag caaaaagttt taggattaaa agattttggt atagatatag
    58981 gtgaaggaag tgtatcattg gaccaagtaa atgaagctga ttgggcaaat gcatggaaag
    59041 catactacaa accaacaaaa gtaggacaaa gggtagtagt aaagcctaca tgggaagatt
    59101 atgctatgca agagggggat ttaatcatag agcttgaccc tggaatggca tttggtacag
    59161 gtacacatga gactactagt atgtgcatta gagaattaga aaagtatgtg aataaagatt
    59221 ctaaagtatt tgatattgga tgtggtagtg gaatccttgc aatagcagca gctaaattag
    59281 gagcaaaaga agttgtagct gtagatttag atgaagtggc tgtaaaagtt gcaaaagaaa
    59341 atgttcttga aaataaagtt gaaaaaagtg tatctgtaat gcatggtaat ttaactgatg
    59401 taataaaaga taaagcggat gtaatagttg caaatataat agctgatata ataaagatac
    59461 ttgcaaaaga tgttcaaaac tttatgaaag aagatgctat attcatatct tcaggtataa
    59521 ttttggataa agtagaagaa gttaaagaaa gcttaattga aaatggattt gaaatagttg
    59581 aagttcaaaa attaggtgaa tggtcagcaa tagtttctaa gcttaaaaaa taagggatat
    59641 ggtgacgtat aatggataga ttttttgttg aaaaaaataa tataaatcta caagataaaa
    59701 cttgcactat agaaggtgaa gatgtaaaac atatttcaaa agttttaaga tgcaaattag
    59761 gtgagaaact tgaaatatgt gacaagaata ataatgagta tatttgtgag ataatgaata
    59821 tagataagag tatagttaat ttagaaattt tagaaaaagt tgatataaat agagaatcag
    59881 aacttaaagt aaggctatac cagggacttc caaaagctcc aaaaatggaa ataatacttc
    59941 aaaaattaac tgaagtgggt gtagaagaaa taatattagt tcaaactaag agaagtgttg
    60001 ttaaagttga tgacaaaaaa gaagataaaa aatttgaacg ttgggaaaga ataatttatg
    60061 aagcagcaaa gcaaagcaaa agaggtaaga taccaaaatt aagaggtgtt ttaagtttta
    60121 aagaagcttt agaagatatg aaaaaaaata gtgtaaatat ttgcccttat gaaaatgaaa
    60181 gaacagtttc aataaaacat gcactaaaaa aatgtgattc taatatagat agtgttggaa
    60241 tttttatagg tccagaaggt ggattttctg aagaagaaat agaacaaata caaaaaaata
    60301 attgtaatgt tgtatcattg gggcctagaa tattaagaac tgagacagct tctgttgtag
    60361 catcaacaat agctttgtat gaactaagtg atttaggagg agaaaaataa attgaaaaaa
    60421 gtagcttttt atacacttgg gtgtaaagtt aatcaatatg aaacagaagc tatgctagaa
    60481 ctatttgaga aagatggtta tgaacaagtc aatagtgaag agtatgctga tgtttatgtt
    60541 ataaatacat gtacagttac tcatatgagt gatagaaaat ctcgtcaata tataagacga
    60601 gttaaaaaga agaatccaga tgcaataata gccgttgtag gatgttattc tcaggtatct
    60661 ccggaagaaa ttttggatat agaagaagta aatttagtca tgggtacaaa tgatagaaga
    60721 aagattgttg aagaaattaa aaagataaat tcaagtaaga aagttagtac agttgatgac
    60781 ataatgaaag ttaaagcttt tgaagaaata gaaatcagcc aaactaatgg aaagactaga
    60841 gcatttatga aaatacaaga tggatgtgac agattttgta cttattgtat cattccttat
    60901 gcccgaggac gcgttagaag tagagatatt gatagtatag tcgatgaagt taaaaaattg
    60961 gctaataatg gctataaaga ggttgtatta acaggaatac atgttgcctc ttatggaaaa
    61021 gatttaaagg atagggatat taaacttctt gatgtaataa aacaaataaa ccagatagaa
    61081 aaaatagaaa gaattaggct tagttctgtt gaaccaattt tatttacaga tgaatttgta
    61141 aatgaagttt taaagatgga taaagtttgt cctcattacc atttatcact tcaaagtgga
    61201 tgtgatgaaa ctctaaaaag aatgaataga cgatacacaa ctttagagta caaaactatt
    61261 gtagatagat tgagaagtaa aatgccagat gtggcaataa ctacagatgt aatagtaggt
    61321 tttcctggag agacaaatga agagtttaaa aaaacgtacg agtttttaaa agaaatagag
    61381 ctttcacaaa tgcatatttt taaatattca ccaagaaaag gaactccagc tgcaactatg
    61441 gaaaatcaag ttgaccctca aatgaaacac tttagaagtg aacaactttt aaatttaagt
    61501 aaagtaaatt ttaataaatt tgcaactaaa tttatcggcc gtgagttgga tgttttattt
    61561 gaacaaaata tagaaggcaa taaatatgag ggattaactt caaattatat aagagttgtt
    61621 gttgaaagtg acaaaaatat acaaggtcag atattaaaag tgaaaataaa tgatgtaaaa
    61681 gatgaatatg tagaaggaat tttgctataa catgtagaaa ttataatatt tataagatag
    61741 gaggtggaaa gaatggattg tattttttgt aaaatagcga acggagaaat accttcaact
    61801 aaagtttatg aagatgatag agtcttagct tttaatgact tgaacccagt tgctccatat
    61861 catatattag ttgtacctaa aaaacattat gatagtttaa ttgatattcc tgataaagag
    61921 atggacattg tgtcacatat tcatgttgtt attaataaaa tagctaaaga gaaaggtttt
    61981 gaccaaactg gattcagagt cataaataat tgtggaagtg atggagggca agaagttaaa
    62041 catttgcact atcatatttt agctggtaaa aagcttccta attacgaagc gggacaaaac
    62101 taaactaaat tattgaaaaa ttgagttcct tcttgataaa aatacttgat aaaagtaaaa
    62161 atttagttta taataaacaa agtgttaggt tgtaatgcat attatgcaaa aagaacatag
    62221 ttacagtccc agcataattg cgctattgga gggagggaaa aagaaatgtc agaagtaaga
    62281 gttagagaga atgaaacatt agacagcgct ttaagaagat tcaagcgtca atgtgcaatg
    62341 tctggcatca tgtcagaagt gagaaaaaga gagcactatg ataagccaag cgttaagcgt
    62401 aagaaaaaag ctgaagcagc aagaagaaaa aacgctaaga aatagtagta aataagaata
    62461 aagaggtgaa ggggatgtcc cttaagcaaa agttacaaga ggatttgaaa tcttcgatga
    62521 aaaacaaaga tacagtgaga aaatctgtag taactttaat aagagcttct ataaagcagt
    62581 atgaggttga taatagggtc gagcttgatg aagatggaat tatcgatgta atagcaaaac
    62641 aattaaagca acgtagagat gcattagttg aatttgaaaa agctggaaga gaagacttaa
    62701 taaaagaaac agaaggtgaa attgaagttt taaaagagta cctacctcaa caattaagcg
    62761 aagaagagtt agaagaaatt gtaaaatcta ctatatccga agtaggggca acttctatga
    62821 aggatatggg aaaaataatg tcagttatcc aaccaaaagt taaaggaaga gctgacggaa
    62881 aacttataaa taagttggta aaacaaaact tacaatagat tgatatagcc tagagagaaa
    62941 tctctaggct ttttatagtt tttgggaata acgagtcata tttgagcata tctataatca
    63001 aagaaaattt tgaaagaagg ggaaaaattt gattatatca gtaaaagaga ttaggaattt
    63061 tttgataaac atatttatac cactaataat aggttactta agtagcatct taacaatgat
    63121 tatcagtgga acagatattt caacctatta tttacaactc acaaagccaa gctttgcacc
    63181 accagctttt atctttccta tagtttggac tatattgtac atattgatgg gtatatcttc
    63241 atacctaata ttaagaaagg gttacaactt acctaaagta agagatgcaa ttttttatta
    63301 tggattacaa cttgtattaa actttatttg gagtatacta ttttttggat ttggacttag
    63361 atttacagct ttgatagata taataataat gattttggtt tctataataa tgatttcgaa
    63421 gttttctaaa atagataaga gatctggata cataaatttg atttatttaa tgtggcttgt
    63481 ttatgcagga tttttgaact attttatatg gtttattaat aaatagaatg taaagatttt
    63541 gtaaattacc ctgtactttt aataatatca taacaaacga aaaataaata gactattaaa
    63601 aataatcatt ttcatataca ataatattaa tttaaataca ataaatttat aaaaattgta
    63661 tttaaagatt aacatattaa agtattagaa ttggaaaggc ttgaagggag aggtttttat
    63721 gcaaataata gtatctggga gacaaatgaa attaactgac ggaataaaag gatacgtaga
    63781 tggaaaatta agtaggttag aaaaatacct tgatcctgaa tcagaagtga aagtaacagt
    63841 aagtgcgaaa aaggatagac aaaaagtgga ggttacaata attcctatta acggtcaaat
    63901 aataagagca gaagatgtag aagatgactt atatgcagca attgatattg tttgtgacaa
    63961 attaagtaga caagttgtca aatataaaac taaagttaaa gacaaagttc aaaataataa
    64021 gagcataaga tttgaaaatc ttgactttat agataatagc agtgaatttg atgattatga
    64081 ttatgatgat gaagaagatg aaaatatagt aatagaaaga agaaagaaat ttaatgtaaa
    64141 acctatgagt tctgaggagg cgatacttca aatggaacta gtaggacaca atttctatat
    64201 gtttagaaac caagataatt ttgaaataaa catagtgtat aagcgtaaag ctggtggata
    64261 tggtttaata gaacaagatt aataaaaatt gtatacgttt agattttata taagaaaaaa
    64321 taaaaaggta atcatgttga ttttttatca atatgattac ctttttcttg tttaaataat
    64381 atattagata ttaggtattt attatataaa aattttaaaa ctaatctgtt acaacatcaa
    64441 taaagtgcat aaatacctat ttcaagcata aaattaatta agggggtaat gcttatgtta
    64501 gaaatatcca ctgatttaca agtaaatcaa ccagttatta ctgttacaag taatacgttt
    64561 attagtatag aaaattatct atcaatatta gaatatgaag ttgatttaat aagaataaag
    64621 actaaagtta aaacaattaa aatatctggg gataaacttt cacttaagta tattactgat
    64681 agtgagatag gtataaaagg gattatctac aatgttgagt atgttgatta ggaggagata
    64741 cacttgataa gttttataag gggatactat gttatagttg tggaaggagt aggactcgaa
    64801 caatttttaa atcatcttat aagaaatggg ataagtgttt acaatgtaac tagaataaaa
    64861 aatactaaaa tggagtttca tatagataga caagatataa aagactttaa gaatgtttat
    64921 agaggaagta agtttgatat aaaggtaaag caaaaaactg gggtcccttt tattatcaaa
    64981 agagtttata agcacaaagg tatgtggatt tgtgctcttg tttctttatt tttattgatg
    65041 tctacatcac agtttgtgac agatgtatat atacaatctc ctgaaggtat aaaaaaagaa
    65101 gctcttagaa atgagcttta taaggttgga gtaaggccag gagtctataa gaagagtatt
    65161 gatagaaaag aagttagaga ccatatgatg tcaaaattta atgatgtggc atatttatct
    65221 atcaatgtaa aaggaacaaa tatatttgtt actgttacaa aaaaggcaga atccctaaaa
    65281 tctacagacc aatcaaatta ttgtaatgta attgctttaa aaaatggtat aatagaaaag
    65341 gtaatcccta gaagtggtaa atcagttatt aaatctggag acatagtcca aaagggagat
    65401 gtcttattaa atggagctaa taccaagtca ataccagaag tatgggcaag tactttttat
    65461 gagtccacaa aaaaggcaag ttatgtagat actgtaaata aaaaaactgg agaaaagaaa
    65521 aatatatata cactaagttt ttatgataaa gagtttacaa tgagaaaaaa tataaaatat
    65581 aaagattatg ttgttgaaaa taaagaaaaa aaactatcaa taggaaacta tacttttcca
    65641 ataaagataa aaacgagtac tttttatgaa acaaaaaagg ttgaagtaaa aaggaataaa
    65701 gaagagttaa aaaaagaatt atctgaaaaa gctttaaaag agttagaata cataatccca
    65761 gcttcggcta gaataataga tgtaaaacat aactttaagg ttaataaaaa tatgttagaa
    65821 tatttaataa cagttcaaac tagtgaaaat attgctaaaa tttatcctct tagtaagtca
    65881 gaagcagaaa ggtttataaa ggaagaaagt aaaccagatg aaggtgaaga ggaagttcct
    65941 tccaatccag aaaaaagacc attaaatgat ataagaaatg agtttgatga ggataataag
    66001 gataataaag accaaaacaa ttctgatgaa aataatagta atcaaaatag taataatagt
    66061 caaaataata atagcaataa caactaataa aattaaactt aaaatcttag gaggactaat
    66121 tcaattgata atacaaaaga aatttacaat agcagatggt aactttgaaa gagagttatt
    66181 tggcaacttt gatgaaaatg taaaattaat agagaagact ttaaatatag atgtaatact
    66241 tagagaaggc aatataattt taattggaga agaaaaaaat gtagattctg cattaaaact
    66301 tatgaatgaa ttacaccaaa ctgtatcaaa tggtaagcat ttagataagc agagtatttc
    66361 ttattcatta tctcttttgc ttgaaggaag tgaacaaaaa ataaaagaac ttgaggggac
    66421 aatcgtcata actcaaaggg gaaaagctgt tcaacctaaa actttaggtc aaaaagaata
    66481 tataaaactt atagaaaata atgatataac ttttggtgtt ggacctgctg gaacaggaaa
    66541 gacttatctt gcggttgcta tggcagtaaa agcttttaaa agagatgaag ttagtagaat
    66601 tatacttaca agaccagctg tggaagcagg agagagtcta ggttttttac caggagattt
    66661 aaaggacaaa gttgacccat atttaagacc actatatgat gccttatttg aaatgttggg
    66721 tgctgataaa tttaataagt atttagaaag aggtactatt gaggttgcac cacttgcatt
    66781 tatgagaggt agaacacttg ataattcatt tataatattg gatgaagctc aaaatactac
    66841 ttctgaacaa atgaaaatgt ttttaactag acttggattt ggctctaaag ctgtagttac
    66901 aggagatgta actcaaacag atttaccaca aaataagaag agtggtctaa tacaagctac
    66961 agagattctt aaaggtgttc caggtattgg gtctataatg ctaacagata gagatgttgt
    67021 aagacatgag ttggtacaga gaataataag agcttatgag aagcacgaca aaagagaaga
    67081 atttaaaaaa gaagaacgta aaaaagcgaa aatgcaggaa aagactttca aaagaaagta
    67141 aaagtgtagt aatatagata tatagcatta ttaatgaata aaaagtaggt gtagtgaatg
    67201 gatttgatac tagatgatag acaagataaa ttagaagtaa gtgaggagtt aattgaaaag
    67261 ataaaggata taatcataga atgtttagat tatgaaggat atgatgataa ttatgaagtg
    67321 agtctttctt ttgtagataa taaagagatt catgaattaa atagagaata tagaggagtt
    67381 gatagagtga ctgatgtttt atcatttcct cttttaagtg atgattttga agatgttgag
    67441 ttagaagaag aatccctagg tgacatagta gtatctcttg aaagagcctt agaacagagt
    67501 atagagtata accatagttt tgaaagagaa gtatgttttt taatatgtca tagtatgttt
    67561 catcttttag gttatgacca cgatacagat gaaaatacaa aagaaatgag agaaaaagaa
    67621 gaacacatat taaataagtt aaacataaca agggagtagt ttttatgaaa ccagaaaaaa
    67681 ctcgtcaagg tataattaaa gcatttaatg cagctataga agggatattg tatacattta
    67741 aatttgaaag aaatatgaaa atacattatt taggttcagt tgctgttctt ataataagtt
    67801 tgttttttaa cttttcaaaa ttagaaatga taatgttact tatgtccata tgtttagttg
    67861 tagttgctga aatgtttaat acagctatag aaaaagctgt tgatttagta actgatgaat
    67921 atcatgttct tgctaagata gcaaaagatg ttgcagctgg aggcgttcta gtggcagcac
    67981 taaattcagt agtagttgga tatattttgt tttatgataa attgacagat atatctggaa
    68041 tattaattta taaaataaga gaatcagaat tacatataac cttgatatgt atattattgg
    68101 ttcttatagc tgttgtagtt gtaaaagcat tgacttctac aggtactcct ctaaaaggag
    68161 gaatgccaag tggtcatgct gccttagcat ttgctatagc aactgcaatt acacttatga
    68221 ctgaaagagt tgttgcctct actcttgcat atataatggc tgtattagtt gcccaaagta
    68281 gaattgaagg aaaaatacat acattttggg agactattgc tggagctttg ttaggtgtgt
    68341 tgatagctat attagtattt cagcttggaa tgttctataa ttaaaataaa tgtacataaa
    68401 atatttaaaa taaaacaaat ctgtgctaat taaattttta tgatataatg tagagtatta
    68461 aataaaaata acttttaatt attgtataca ggaggagttg ttgtggataa catagaattg
    68521 ttaaggttgg cagaagatgc cagacaacat tcttatgctc cttactcagg ttttagagta
    68581 ggtgcagctc ttttaactaa gagtggtaaa gtttatactg gatgtaatat agaatgtgca
    68641 tcattaggtg gaacaaattg tgcagaaaga acagctattt ttaaagcaat atcagagggt
    68701 gataaggata tctataaaat agctatagca agtgataact cagaaaataa tgagcaaaca
    68761 tatccatgtg gaatatgcag gcaggtaatt atagagtttg gttctgatat aaaaataata
    68821 acgggatata ctaaaggaga ggtgtttgag catagtataa aggatttgtt gccaaactat
    68881 ttctctggaa atgatttaaa ataaatttaa aagtataaat aaatttaagt aattaaaaca
    68941 agaagataaa aagttaggag acagttaatg ttcaaatcag gttttgtcag tatagtgggt
    69001 agaccaaatg taggaaaatc tactttaatg aataatgtag ttggagaaaa aatagctata
    69061 atgagtgata agcctcaaac tacaagaaat acaatacaag cagtatatac agatgaagaa
    69121 atgcagatag tattcttaga tacaccaggt attcacaaac caaaaaataa attaggtgaa
    69181 tttatggtaa aggcagcaac agaagctttt aaaaatgtag atttaatatt atttgtagtt
    69241 gatgattcta aaaaaatagg tcctggagat agaaagataa tagaggattt aagaagtgtt
    69301 aagacaccaa taattttagt tgtaaacaaa atagaccaac tagaccaaaa agatgagcta
    69361 tttgatataa tcaaaatgta tgatagagaa ggtattttta aagaaatagt accaatatca
    69421 gcattaaaag gaaagaatac ggatacctta ataaaagtta tacaaaatta tttagaagaa
    69481 gggccaaagt attttccaga ttatatgatt acagaccagc cagaaagagt attaatagca
    69541 gaacttataa gagaaaaagt tcttcactat cttaatgatg aaatacctca tggtgtagct
    69601 gttgaaattg aaaaaatgaa agcgagaaat gataaggaaa tagtagatgt atctgctgta
    69661 atatactgtg aaagagattc acataaggga ataataatag gaaaaaatgg taggaaacta
    69721 aaaggaatag gtaagtcagc aagacaagat atagaattgc ttttaggttc acaaataaat
    69781 cttcaattat gggttaaagt caaggaaaat tggagaaatt tacaaaatta tataaataat
    69841 tttggatata atgataaata aaggtgggtt atatggatag gaaactggat gcttcccaag
    69901 atttattata tgaagtaaaa tcattaattg ataataataa agtattggag ttaagagaat
    69961 taatagagga atatcatata attgatatat tcgatataat ggaaaattta gaggaagaca
    70021 tgaaaattca attatttgaa gtacttcctt tagatatggc atcttctata ttagaagaag
    70081 gtagtgtaga gttttttatt agtattttat ctaaactgga tgtagaacac tcgaaaaata
    70141 tacttgaact tatgtctctt ggagatatgg cagacaagtt gagtgaactt gaagaagaag
    70201 aaagagaaca cataataaat cttttgaatc aagagaatgc cgattatgta aaagagttac
    70261 tattctatga tgaagattct gctggtggaa caatgaccac tggatatatt tctataaata
    70321 aagatatgac agctcttgag gctatagacc atatgagaga agaagctgaa gaagctgaaa
    70381 ctatttatta tatatatgtg gttgatgatg aagaaaaact agttggagta ttgtcattaa
    70441 gagaattaat aatagctaga gacgcaaata tagtagaaga tttgatgagt gaaaatataa
    70501 tctctgttta tgtagatgaa gatagagaag aagcagtaag attagtatct aaatacaatt
    70561 taatagcaat tccagtagta gacagacaag aaaaattaaa aggaataata acagtagatg
    70621 atataattga tgtaatggaa gaagaagcaa ctgaagatat gtataaattt gcaggaagtt
    70681 cagaacatga aagagaagtt gcggaaaaag aaaaccctac attgagagag caaataatat
    70741 cagcacttag aggacgatta ccttggttaa ttattacctt agttggaggg ttgttggctt
    70801 cgttaatact ttctaactta gactatataa tgaatcctgt atatgctcca ttagtatttt
    70861 ttatacctgt agtaattggt atgggtggaa atattggaac tcaatcatca tctgttacag
    70921 taataacatt gtctaataag gatttaaatt ttagcaatgt ggttagagaa ggtattgtag
    70981 gtattattac aggtcttttg tgtagtataa taacaggtat tgtaatttac tttgttatga
    71041 gaaagttaga cattgtatta attgtttcaa tatcattatt tataaatatg gttttaggtg
    71101 caacaatagg tgcgtttatg cctgttttat taaaaaaaat ggatgcagac ccatctacag
    71161 tttcatcacc aattatatct acagctcttg atataactgg tatagcagtt tactttataa
    71221 taacaacggc attattgtca aaaattgttt aataacttta tgtatgattt atcctcttct
    71281 tactaaaaat ataagtataa atgtaagaag ggggtttttt aaaaatgaac aatatcagtt
    71341 gggaagtttt taaaaaaaca ggtagtatag atgcatattt atatttctgt gattgcaaaa
    71401 acatacatga agaaattgaa gaagtaaggg agaaaaaaga agacgatgat aatcctgaac
    71461 acccagggga tagtacttaa agctataagg tataaggaaa gtgatataat acttacacta
    71521 tttacaagaa agcttggaaa agtatctgca atagcaaaag gagctaaaaa aaataagagc
    71581 tctttgcttt catcatcaca gttattttct tatagcaatt ttaccttaaa aaaacaaggt
    71641 aatatgtaca aggttactca aagtgaaata ataaaaagtt tttataatat atcctatgat
    71701 atagaggcat tttcttatgc tacatatatt acaaaattag ttgaaaattc tatacttgaa
    71761 aatcagacta ataataggct atttatatta cttgcgcaga ctctgtattt gtatacacag
    71821 gataatacag acaataggtt tataacagca gcattcgagt taaaattttt agactatata
    71881 ggatttaaac ctatagtaaa taaatgcatt aattgtgaat gtaaaatttt acaaaattct
    71941 gtgtttaata tctacgaagg tggtatacta tgtagtaagt gtagtaaact ttttgataat
    72001 aatataaaat tagacttaac aactataagt cttatggaat atgtattaag aaatgatatt
    72061 ttaacatgta gtaaagctaa agtatcaaaa tatataacac atgaacttga gaatattcta
    72121 gacaagtatt tgaaagttta tgttgataat attaatttta aatcattaca cgtattgcaa
    72181 agtgtaaaaa ataataaggg agtggataat gatgagtagt aatataacta ttgaaatggt
    72241 tgattcagtc tttgaaagag ttccaggagc aacttatgca gaagcaaaag aagcattagt
    72301 aatgtgcaat ggagatgtaa ttgaggcagt aatatattta gaaaataaaa aaaatacttg
    72361 taattgtaaa gcaaaatctg ctaaagaaac tatggaagaa gtatttggca aagatggaga
    72421 aaatataaag tttcaattaa aagagcttct tagaaaaagt agtgtagtgc gaataatagt
    72481 tgaaaaagat ggaaaaacta tgatgaacat tcctttaaca gttggagttg ttggattagc
    72541 ttttgtacca ttagctactt tagttggatt atcagctgct gttataagta aatatcgtat
    72601 aaaagttcaa aatgaagaag atggagaaat tgtagattta ggtgaattga atgaagaaaa
    72661 acttcacgtt ttgaagaata tgataactaa tgcagcaaaa gatgtgaagg atgtagtagt
    72721 tgacaaaaaa gaagatgaca aagatgtaac tgctgacctt atgaaagaag aatctgaaaa
    72781 gaataaagaa gacgaagacg aaaataaata gatataaagt gacttttaaa aaatgaagag
    72841 aagataatta taagaattat cactaacatt gacaaatttt atagaatttg atattctaaa
    72901 agttgtagaa gaactattaa aatagccttt cgtgtagacg agaggcttta ttgttatagg
    72961 agggattttt atgaattttc aagagatgat actggcgttg caaaaatact ggtcaaagca
    73021 aggttgtatc atgatgcagc cttatgatat tgaaaaaggt gcaggaacta tgaatcctaa
    73081 tactttttta agatcattag gacctgagcc ttggcaagta tgctatgtag agccttcaag
    73141 aagaccagca gatggaagat atggtgaaaa cccaaacaga ctatatcaac atcatcaatt
    73201 ccaagttata ctaaaacctt ctccagataa tatacaagaa ttatatttgg aaagtttaaa
    73261 agatattgga attgacccaa gtgagcatga cataagattt gttgaagata actgggaagc
    73321 tgctacagtt ggtgcttggg gacttggatg ggaagtttgg ctagatggta tggaaataac
    73381 tcaatttacg tattttcaac aagtaggtaa tattgaatgt gagcttgaga caggagaaat
    73441 tacatatgga ttagaaagat tagctatgta cattcaagaa gttgatagtg tttatgattt
    73501 aaaatggaat gataaaataa cttatggtga agtatttaat aaagctgagt atgaaaactc
    73561 aatgtacgca tttgaattat gtgatgcaga tatgttgttt aatctgtttg atatatatga
    73621 aaaagaagca ttaagactta tggaaaatgg ccttgtaata ccttcatatg actatgtgtt
    73681 aaaatgctct catgcattta atacattgga tgcaagaggt gcaataggag ttagtcaaag
    73741 agcttcattt ataggtagag ttagaaatat ggcaaagact gttgcagaaa cttttgtaaa
    73801 acaaagagaa gaaatgggat tccctctttt aaaggacggt gacaaataat gaaaaattat
    73861 cttttatttg aaatcggtgt tgaagaaatg ccatcaagat ttgtaggcag tacactagaa
    73921 caattaaaaa acaatcttag taaattattt gatgaaaata gaatagcttt tgataaaata
    73981 aatgcgtatg ggacaccaag aagacttact ttagtagtcg aaggattgag tgatagacaa
    74041 aatgatttag aagaggaaat aaagggtcct gctaagaaaa ttgctgttga tgcagaaaat
    74101 aatttaacaa aaccagcatt aggatttata aaaagcaaag gattaagcga atctgacata
    74161 tattttaaac aagttggaaa agaagaatat atatttgcga ctataaagca agatggaaaa
    74221 gaaacttcag aagtactaaa agacatatta ccagagacta taaaatctgt agtattccca
    74281 aaagctatgc gttggggagg aaaaaatctt aaatttgcaa gacctataag atggatagtt
    74341 gcattgttaa atgataaagt attagagttt gatttagaag gaataatatc ttctaatgta
    74401 acaaaaggtc atagattttt aggtgaaagt acaatagaag ttgattctat agaagattat
    74461 tttgaaaaat tagaaaaaaa ttatatagtt ttagaccaaa acaagagaaa agaaatgata
    74521 agaaaacaat gtatagaagt ggcaaattct ttaggtggag aagttgaatt tgatgaagat
    74581 ttacttgaag aggtaactta cttagttgaa tatccaactg ctttttatgg agaatttgat
    74641 gtggattata taaaactacc aaaagaagtt gttataactc ctatgaaaca acatcaaaga
    74701 tattttccag tacttaaaga aggaaaactg ttacctaact ttatagctgt tagaaatggt
    74761 gattcttaca gaatagacaa tgttaaagct ggaaatgaaa aagttttaga agcaagactt
    74821 gcagatgctt tatttttcta tagagaagat actaagagaa acttagaaag ctacatagaa
    74881 aaattaaaaa ctgttgtatt ccaagtaaaa ctaggaacta tttacgacaa aactttaaga
    74941 cttgaaaaat taagtgctga tatacttgat agtttaggtt tagtagatga aaaggcagat
    75001 actataaggg ctgctaaatt atctaaggca gatttagtta ctggaatggt aggagaattt
    75061 gatgaattac aaggttttat gggaaaagaa tacgcaaaag ttggaggaga aaatgatgtt
    75121 gtaagtgaag ctatttttga acattatctt ccaagatttg caggagatat acttcctaag
    75181 actaatgcag gagttgcctt atcaatagct gataagttgg attcaatagc aggtttcttt
    75241 gctataggaa tacaaccaac aggttctcaa gacccatatg ctttaagaag acaagcttta
    75301 ggtatactta gtatacttat ggacagaaaa gttgctgtgg atataaaagt acttattgaa
    75361 catgctttag acaattattc tgaattagat tttaataaag aagaagttgt tgagcaaata
    75421 atgaatttct tcaatgagag agtaaaaaat ctatttaaag atttaggaat aagatatgat
    75481 gttgttgatg ctgttttaag ttctgatata aatgatgttt ctgatatgca tatgagagct
    75541 ttagagctta ataattggct agaaaaagat gaattagtgg agatgcttac tgcatttaat
    75601 agagtttcta ctctagctca aaaagcagaa agtgataaaa ttgatgaatc tttattaaaa
    75661 gaagatgctg aaattaaact ttacaatgaa tttaaacaag taaaggtaaa agttaatgaa
    75721 ttattaaagg ataagaaata tggtattgct ttagatgaat ttgcatcttt aagaccagta
    75781 gttgataatt tatttgatac agttatggtt atggataatg atgaagctat taagaacaat
    75841 agacttgctt tacttaaaca agtatatgat actatgctag gaatttgtga tttatctaaa
    75901 attgtttata aataaaattt aaactattta taagaaataa ggctgtctca aaatagagat
    75961 agtcttattt ttcattgttg agatgatgaa aatatatttg atttcataga aaaagagtgt
    76021 ctcataaact aaaaagttca ttttgagaca cccatgcttt ttgtaagtaa atataataaa
    76081 ttatatttta tacttatcag ttattttgtt taaaatatta tcatttgaat ttacccattc
    76141 ataaaaattg cctgatgatg cgtccgatat tttataaagc atttcaggaa tatcactagc
    76201 tttaaaatct ccaagtgatt ttcccaatct agtcttacct atttcaaaac tgcctccaac
    76261 aaataattca aatgcatctg tagatatacc atcaacattc ttctttttac cacataaacc
    76321 tatgctacca acttgatgaa caccacatga atttaaacat cctgatatat ataacttagg
    76381 tgctttattt aaaatttctt tattattttg taatctaaaa taatctatga tttcatgtaa
    76441 cattttctga ctattttgta tgcccatttg acaaattgga acacctatgc aagatatact
    76501 ttgttctaat tgagaattac aactaatagt tttacttatt tctaatattc tttttgcttc
    76561 atttccattt aaatttaaaa tatataatcc ctctgtcatt cctagtctaa tcataggatt
    76621 ttcaatatta tctaattctt taagtaaagt acttaaatcc tttgttaaca aaatacctcc
    76681 aactggatgt atatatacag tgtataaacc atcctgtttt tgaggtatta gtctagaatc
    76741 ctgtatatct attttaaccc caggtttaga gtaatctatt tcttcaatat tgagttcaag
    76801 actaccattt tctttttcta atttatagta ttctttaaat ttttctaaga ataatttttc
    76861 accaagtgat tcgaccatat atctaaccct agctctgttt ttattttcat aatttccata
    76921 atccataaag aatttaatca taccttcaac acaatataag gcatctttcg gttttataag
    76981 ttcatcaagt tctaaagcaa ctttgggttg tttacctaga cctccaccta agaaaactct
    77041 gaaatatggt tcatcatttt tcaaagtagc aacaaatcct aagtcttgta tactacaatg
    77101 agcagtatct gtgtaacaat tagaataaga aacttttaat tttctaggta aatggtatgt
    77161 agtagcattt ttgataaaat atttatcagt agcaactgca tatggagtaa catcaaagac
    77221 ttcactaggg tcaactccag ataatggaga taatccgaca tttctaggat aattaccacc
    77281 accacctcta gtgaatatat cattttttat tccttcttcc atgatatcaa caattgaatt
    77341 tatagataaa tcgtgtaatt gtatagcttg tctagttgtt aagtgtatct tatctaagtt
    77401 gtgtttaata gctatttcat agattttatt aagttgatac tgagataaaa ctccagcaga
    77461 tactctaagt cttatcataa aagatttttt gtctctttga gcataaacac catatccacc
    77521 agaaaatccc ttatattgca ttttatttat ttctccattt aaaaattttg aagaatattc
    77581 tttaaaatta ggtagttcat ttaacaatat ctttttaaaa ttatccatta tttcacctca
    77641 ttgttagtct tgtataaaaa tatatctatt gtataataga tatatatatt attatctagt
    77701 caggataaaa atataacaga ataattctat taaaagtaag aataataagg acatagtgag
    77761 tattttgttt ttattttaca aattaagtat aatatttttg ttttataaaa tataattaag
    77821 ataaatagat aattttgtaa ttttaatata taatatttag tagagtgtgg gagtagcact
    77881 taatatttga aggaggagtt tttgtatatg ggagaaataa taaatgcatt agatagagcc
    77941 ctagacataa ttttgctgtt atatcatgaa aagagagaaa tgggaataac tgaaataagc
    78001 aaggctatgg gagtatataa aagtactgta catagaactt tggttactct tgaaaacaaa
    78061 ggttttgtta tacaaaatgc agagaatggt aagtattggc taggaataaa tttatatgca
    78121 ataggtatgg ttgtgggaga aaaaatgtca ttgactgaaa tagtaaaacc atatacgaaa
    78181 aaattaaatc aagaatttaa cgaagtagtt aatgtatcta tattagaaga aagagctcaa
    78241 gattctccaa gaagtatcat aatacataaa gagtatggtt caaatcaatt actttcagta
    78301 aatccatccg ttggttcaag tagtgaatgt tattgttcag cagttggtaa atgtcttatg
    78361 gcatttaatg attctataga ttttgaaaaa tatagaaaaa caccaataca caagtataca
    78421 gaacatacaa ttgataattg ggatgatatg atgctttttt tagcaaaaat aaaagaacaa
    78481 ggatatgcta tagatgatga agaactagaa catggactaa cttgtattgg agcacctatc
    78541 ttagataaaa ataataaagc tattgcagcc ataagtttgt caggtccaac tataagaatg
    78601 agagaagggg actttgagta taagattaaa agagttatag aaactgcaaa aagtatatct
    78661 gaacttttta gatgattttt aatctcgata ataaaaatag gctatattta aattgttatt
    78721 tgtaaatatc agtttaaatg tagcctattt ttctttaatc taaaattaaa tattcttaaa
    78781 atttaaaatg ttattgaaat atataagcga atgtgttatc ttttaattaa gcaataaaaa
    78841 tagaacaagg ttctatacta tagaacaaaa ttgatgcata tatagtattt atacaaaata
    78901 taaatattat atatttaaaa ttaagtaaaa atattacata aaggggagat ttgagaaatg
    78961 gaaaaagtca agttgagtgt tgaaagatgt aagggatgct atctttgtat tgaagcttgt
    79021 aaaaaagatg caataagtct ttctgactac acaaataaaa aaggctttgt aactattaaa
    79081 gtcgatgaag aaaaatgtat tcaatgtggc gcttgttata caatgtgccc tgatttagta
    79141 tttgagatat tataaattat ttttattaga ggggagaaaa ggtatgcaaa gtttattaat
    79201 taaaggaaat gaagctttag cagaaggtgc tatacgtggt ggctgcagat tttatgcagg
    79261 ttatccaata acgccacaat cagaaatatt agaaatgatg gcatggagac aaaaagaagt
    79321 tggaggagta tttatccaag gtgaatcaga aatagccagt gtaaatatgg ttatgggagc
    79381 aagtgcactt ggagcaagag ctatgacttc ttcatctggt ccaggatttt cattaaaaca
    79441 agaaggtatt gcatattggg tatcagctgg tttgccagca gttgtagcat gtgtgcaaag
    79501 atatggtata ggtgatggat ttataggagc tggtcaagat aattattggc aagcagtaaa
    79561 aggcggtgga aatggagatt atcatttaat cgtgttagca cctaatagtg ttcaagaaaa
    79621 tatggatatg gcatatgaat cttttgaatt ggctgaaaaa tatatgcatc cagtacttat
    79681 actttctgat gctacaatag gtcaaatggt agaaccttgt atagcaccac caatgaaaga
    79741 atacgatatg gataaagctc catgggctgc aaaaggaaca cctgtaggag aaaaaaataa
    79801 agtaataact gatataacat attttgaaga ccaagataca tggcaaaaag ggtatataga
    79861 aaaaatgaga aaagttcaag aagatgaaca aagatgggaa gagatagaaa tagaagatgc
    79921 tgaagtggta tttgtggcat acggtatttc atcaagaata gcagaaggtg cagttagaga
    79981 tgctagagca aaaggaatga aattaggtct aataagacca aaaacattgt ggccttaccc
    80041 aagaaaagca tttaagaaat taagtgattc attgaaggga cttgtaacag ttgaaatgaa
    80101 catgatggca cagatgaagg aagatgtaat tatagcatgt gataatagat ttccagtata
    80161 ttcagtagca acagctcaat atatggcaga agtaaatcaa ttaatagatt ttgctcaaca
    80221 gattatagat ggaaaagcta agcaagagga ggtattttaa ttatggctaa aaaagcacca
    80281 gcaataatga tgtgtgaaaa tatgttctgt ggaggctgtg gacactctat agtcgataga
    80341 attattggag aagtattaac agaaatggat atatgtcaag atacaattat atgttcagat
    80401 ataggttgta atcatatgtt tcaatttagt atgaatgtag atgttgtcgt tccacctcat
    80461 ggtaaaatgg gagcagcaat ggcagctatg aaaagagtta gaccagataa atatatattt
    80521 acaatagctg gagatggtgg agcttatgca atagggcttg gagaaactat gtcagctgct
    80581 actagaaatg ataatgtaat atttcttgtt atgaacaata atatgtttgc aatgacagga
    80641 gggcaaatgg ctccaactac tatagaaggt caatatacag cttctactcc agcgggtaga
    80701 aattacgcag atcatggttc aacatttgat gttgtaaaag ttatgggaaa tttagatata
    80761 gcatatctag caagaggaac aataactaac cctaaagaaa taaatacaac aaaaagatac
    80821 atcaaaaaag ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt
    80881 gtaccatgtc caacaaactg gggattaaag ccagtagatg ctatgaagag aattgaaaat
    80941 gaacttgttc caaagtatcc acttggagaa tttattgatt agggaggaaa taatatgttg
    81001 aatgaaatta tatgtgctgg ttttggagga cagggagttc ttacagcagg aaaaattata
    81061 atgtatgttg catataaggc agaaaagaaa attacatggt tcccatctta tggaaatgag
    81121 atgaggggag gaagtgcaaa ttgcaatgtt gttgtaagtg atgaaaaaat tgcaaatcct
    81181 tgtgtaaaaa atccagatat actattggga atgaacagcg tatcagtaga taagtatgaa
    81241 agtttaatga aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag taaggataga
    81301 aagtacagag aagacattaa tgttataaaa gtacctgtta ctgaaatagc acaaaattta
    81361 ggcaatgaaa aagctgctaa catttgcatg ttaggtgcta tggtaaagag tttggattta
    81421 tttacaaaag aagaatttga aagtggtatg tgtgaatact ttgaaaatca aggcaaaggt
    81481 aaattcaatt ctaaaaatgt agaagcattt gaagctggat acgactacga atgaatattt
    81541 taaatattta aaataattat tgaaatactt atttttatat gttagtattt aattaataaa
    81601 atatagaacg tcgttctgga caatgggacg aagtctttag gtcggaatac aagaacggcg
    81661 ttctgtataa tagaactagg gggtgttaaa ttgaaagttt tagtaataaa tcctggtggt
    81721 acatccacaa aaatttctgt tttccaagat gaaaatgaaa ttttgaaaaa aaatataacc
    81781 catacaagag aagatttaaa aaatttttct aaagtatttg atgaatatga ttatagaaaa
    81841 caactgatag tagatatttt aagtagtgag aatcatagta taaattcttt tgacgcagta
    81901 gttggtcgtg gtggacttat gaaggctata aaaggtggta cctatactgt gagtgaagaa
    81961 atgattgagg atatgagaaa tgaaataaat ggggaacatg catcaaattt aggtgcatta
    82021 ttagctaaaa ctatagctga tgaaatagga gtacaatcat ttgtagtaga cccagtttct
    82081 gtagatgaat ttgatgatgt atcgaggatt acaggaatat cagacataga aaaagcaagt
    82141 tggttacatg cattaaacca taaagctgtt tcaagaaaaa ttgcacaaaa actaggggga
    82201 aaatacgagg attataactt tattgtagct catttaggtt caggaatatc gattgtagca
    82261 cataaacaag gcaaaatgat tgatggaagt ggtggtaggt ctgatggtcc attttctcca
    82321 gaaaggagtg gtggattact tgcatatcct cttatagaat tgtgttactc cggaaaatat
    82381 actcatgatg aaatggtcgc aaaagtaagt tcgattgggg gaatgtacga ttatttagga
    82441 acaaaggaca tgatagaaat tgaaaacaga atcaaggagg gtgatgaaaa ggcaaaactt
    82501 ataatggatg catttattta tcaagtagca aaagaaattt ctatgtatgg agcttcactt
    82561 tcagggaatg tagatagagt tatcttgaca ggagggatat ctcactcaga aatggtagtc
    82621 aaaggagtag cagaaaaagt ttcatatcta gctccaatag agatagtacc aggagagatg
    82681 gaaatggagg cacttgctct aggggcactt agagtactta atggagaaga aatagcaaaa
    82741 acttattaat atttaggaga aagagtatgt taaaagattt taataattta ttagaagagg
    82801 ctaaaaagct tgaacctgtt attgtaagtg ttgctgttgc agaagacaaa gaagtattaa
    82861 aggctgtgaa atcagcaaca gatttaaaca ttataaagcc aattcttgta ggagacaagg
    82921 ataaaataga aagtatatca agagaaattg gattaaaagg tcatgaaata ataggttgtc
    82981 aaaatcccga tgaatcagtt aaaaaagctg tagagatagt taaaaataag gaagctaaag
    83041 ttcttatgaa gggattagta aatacaagtg tctatatgag agctatatta aataaagaaa
    83101 cgggtcttag aacaggaaga ttattaagtt tgcttgcagt atatgaatta cctcaatatc
    83161 ataaacttct ttattgtact gatagtggaa taaatgtatc gccaaactta aatcaaaaaa
    83221 aagatataat gacaaatgca cttcttacta tgaaaagtat aggcatggaa aatcctaaaa
    83281 ctgcgatact tactgcaaat gaaatggtag accctaagat acaatctaca gtggatgcca
    83341 acctacttgt agagatggaa aaatcgggag aaataccaaa gtgtatagtg gaaggtccaa
    83401 tagcatttga tgtagcattt gatgcacatg cagcagaaca taaaggtatt aaaagtaata
    83461 ttgctggtga tgttgattta ttagtatttc caaatataga agcagggaat atattaggaa
    83521 agtcatggct tagatttaat aaagctaagt gggcaggtat agttttagga gcaacaaatc
    83581 cgataatttt aggttcacgt tctgacacag ctgaaataaa aataaattca atagcattag
    83641 cgtgcctagc atcacaataa gaaattatta ttaaggagga aaaagtatta tgaaatatgc
    83701 aaaaagaatg gatatggtaa aagcttcagc tataagagat tctcaaaaga aaatagctca
    83761 gaaggtagca agtggtggaa ctgttatatc ttttgctgca gggcttcctg acccaaattt
    83821 atttccaata gaagagataa gtgaaataac tcaaaaagta cttaatgaaa ggggaaaatt
    83881 tgccctagca tatggaccta ctaaaggtga agatgagttg ttagatctat tagtaaaaag
    83941 aatgaaagaa gaagaaaata ttgatacaaa agcagaaaat attataataa ctactgggtc
    84001 acaacaaggg atagcattaa gtgctatgat tttattagaa aagggagatg tagtagttac
    84061 agaaaatcca agttacttag gtgcaataaa tgcatttaga ccttatgaat gtgatttttt
    84121 aggagtagat acagatgaag aaggaatggt aacagatgat ttagacaaat tattatctga
    84181 aaattctaac ataaaggtga tatatgtaat accaacattt caaaacccta caggaaaaac
    84241 atggtcttta caaagaagaa aagattttat ggaagtcgtg aataaatatg atgtcatagt
    84301 aatagaggat aatccttatg gagaaataag atttacacca gaacctctac ctacacttaa
    84361 atctcttgat actaaagata aagtattgta ccttgggtca ttttcaaagg tattatgtcc
    84421 aggatttaga gttgcttgga tgtgtggtaa tgtagacatc atagataagg cagaactttt
    84481 aaaacaaggt atagatttac agtcaaatca attttcgcaa gtacaagtaa tagaattttt
    84541 aaagaaatac aacttaaatg aacatataga aaaaataaga gcagagtata agaaaagatg
    84601 tctgctaatg ttagatgcta tgaagaaaca tttcccagag gaagctaaat atacagaacc
    84661 agatggagga atgtttatat gggttgaact tcctgaaagt ataaatgtag atgatttatt
    84721 agataaagcc attgatgctg gagttgctta tgtttctgga gagtccttct ttgcaaataa
    84781 tggtccaaaa aatactatga gacttaacta tacaacaatg tcagaagaac aaatcgttaa
    84841 aggtgttgag atacttgcag aagttataaa aaaagaactc aattatgctt gtgaataaat
    84901 ataaaaagtg agggtttaaa tatgggagaa aaagaaaacg gtataaagtt tggaaagtta
    84961 atgtttcaac ttgttattat agcaatatct tgggaattag tttatattat accatttatc
    85021 caatatacat tatatgatcc aatcctaaaa gcattagaat gtacaaacac tcaactaggt
    85081 tttctactta caatttatgg gttaggtaat atatttggag ctcctatagg aggctggtta
    85141 gcagatagat ttgattatag aaagataatt ttaggttctg tatttcttaa tggagtagta
    85201 tcatttttat ttgcatttaa tatgaactat ccatttgcag ttgctacttg ggtaggttgt
    85261 gcaatcacat cattagttat gaactatcct tctatggtaa aaatacttag agttatagga
    85321 aaagataatc aaggtaaggt ttatggattt aatgaagcta tggttggagt ttctggagta
    85381 gtaatgggag caatattctt atatatttat acatgctttg caactcctac attgggtatg
    85441 agatgggtaa tgatatcttt aggaatatta tcaatagcaa tgtgtccagt attatggttt
    85501 gtaattaaag atgtagatat taaagaagaa agagaagaag caggtgaaaa aatgtctgct
    85561 ggtgatttta taacagtact aaaatcacca aatacttggc tagtaggaat aagtatattc
    85621 tgtgtatatt cattcacagt tacaatgtct tattttacac catatataac atctgtactt
    85681 ggaggttctg tagcattatc tggtgcatta gcaataataa gacagcatgg tctaaaatta
    85741 tttggagcac catttggagg atattgtgca gacaaattaa aatctcctac aaaagtactt
    85801 ttaccaatat atgtatttgg tatagcagta atagttttat tcctagtatt acctgcatca
    85861 actccaatga ctatattcat agctttaaca tttgtagtag gtatattagg ttacatggga
    85921 aaaggtatat attatgcagt acaagatgaa gtaaaagttc cagttaaata ttcagcaact
    85981 actataggta tagctgcagc tttaggattt tcaccagatg tattccaatt tgcattaata
    86041 ggacactgga tagatactta tggcaataaa ggttacacat atacatttat attccaaata
    86101 gcaattctag ttattggaat actatcttgt ttatatatat taaaggttaa aaagagaaga
    86161 gcaagtaaat tagaagctta aatattcaat ttggtaatta agtatatata aatacaatac
    86221 ttattttgat gaatgtgatt gtataaataa cataaaaaat atcaatagat taagttttat
    86281 aataatgttt agagaataaa gtataaaaat caaaatgtta tcaagttttc taggttttaa
    86341 cataaagtta cccctgaagg tgtaaaattg agtggcaatt attatctatt ttcttaccta
    86401 cataaaccta tttaatttaa attctaagag attttcaaaa tagatggtag ttgtactcaa
    86461 ttttaaacct cataataaat acgaaaattt aaaatctaac tagatactat ttaattatat
    86521 attaataaat atata
//
LOCUS       DAMJYX010000005        84393 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJYX010000005 DAMJYX010000000
VERSION     DAMJYX010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 84393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.005"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            273..806
                     /gene="pyrR"
                     /locus_tag="PC479_000353"
     CDS             273..806
                     /gene="pyrR"
                     /locus_tag="PC479_000353"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2802942.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1090..2301
                     /locus_tag="PC479_000354"
     CDS             1090..2301
                     /locus_tag="PC479_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2802943.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2600..4252
                     /gene="abc-f"
                     /locus_tag="PC479_000355"
     CDS             2600..4252
                     /gene="abc-f"
                     /locus_tag="PC479_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HDF2802944.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4467..6242)
                     /locus_tag="PC479_000356"
     CDS             complement(4467..6242)
                     /locus_tag="PC479_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2802945.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6538..7572
                     /gene="ltaE"
                     /locus_tag="PC479_000357"
     CDS             6538..7572
                     /gene="ltaE"
                     /locus_tag="PC479_000357"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2802946.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7859..8689
                     /locus_tag="PC479_000358"
     CDS             7859..8689
                     /locus_tag="PC479_000358"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2802947.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            8693..9574
                     /locus_tag="PC479_000359"
     CDS             8693..9574
                     /locus_tag="PC479_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2802948.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9604..10221
                     /gene="gmk"
                     /locus_tag="PC479_000360"
     CDS             9604..10221
                     /gene="gmk"
                     /locus_tag="PC479_000360"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2802949.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10224..10490
                     /locus_tag="PC479_000361"
     CDS             10224..10490
                     /locus_tag="PC479_000361"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2802950.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10495..11694
                     /gene="coaBC"
                     /locus_tag="PC479_000362"
     CDS             10495..11694
                     /gene="coaBC"
                     /locus_tag="PC479_000362"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2802951.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11933..14458
                     /gene="priA"
                     /locus_tag="PC479_000363"
     CDS             11933..14458
                     /gene="priA"
                     /locus_tag="PC479_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2802952.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14476..14916
                     /gene="def"
                     /locus_tag="PC479_000364"
     CDS             14476..14916
                     /gene="def"
                     /locus_tag="PC479_000364"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2802953.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14931..15860
                     /locus_tag="PC479_000365"
     CDS             14931..15860
                     /locus_tag="PC479_000365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2802954.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15870..16622
                     /locus_tag="PC479_000366"
     CDS             15870..16622
                     /locus_tag="PC479_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2802955.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            16691..17392
                     /locus_tag="PC479_000367"
     CDS             16691..17392
                     /locus_tag="PC479_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2802956.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17454..18779
                     /gene="rsmB"
                     /locus_tag="PC479_000368"
     CDS             17454..18779
                     /gene="rsmB"
                     /locus_tag="PC479_000368"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2802957.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18787..19818
                     /gene="rlmN"
                     /locus_tag="PC479_000369"
     CDS             18787..19818
                     /gene="rlmN"
                     /locus_tag="PC479_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2802958.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19838..20590
                     /locus_tag="PC479_000370"
     CDS             19838..20590
                     /locus_tag="PC479_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2802959.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20607..22622
                     /gene="pknB"
                     /locus_tag="PC479_000371"
     CDS             20607..22622
                     /gene="pknB"
                     /locus_tag="PC479_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2802960.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22730..23467
                     /locus_tag="PC479_000372"
     CDS             22730..23467
                     /locus_tag="PC479_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2802961.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23565..24449
                     /gene="rsgA"
                     /locus_tag="PC479_000373"
     CDS             23565..24449
                     /gene="rsgA"
                     /locus_tag="PC479_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2802962.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24454..25125
                     /locus_tag="PC479_000374"
     CDS             24454..25125
                     /locus_tag="PC479_000374"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2802963.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25134..25775
                     /locus_tag="PC479_000375"
     CDS             25134..25775
                     /locus_tag="PC479_000375"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2802964.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25906..26535
                     /locus_tag="PC479_000376"
     CDS             25906..26535
                     /locus_tag="PC479_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2802965.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26590..27246
                     /locus_tag="PC479_000377"
     CDS             26590..27246
                     /locus_tag="PC479_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2802966.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            27660..28376
                     /locus_tag="PC479_000378"
     CDS             27660..28376
                     /locus_tag="PC479_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2802967.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            28351..30684
                     /locus_tag="PC479_000379"
     CDS             28351..30684
                     /locus_tag="PC479_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2802968.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(31001..33679)
                     /gene="mngB"
                     /locus_tag="PC479_000380"
     CDS             complement(31001..33679)
                     /gene="mngB"
                     /locus_tag="PC479_000380"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2802969.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(33754..34860)
                     /locus_tag="PC479_000381"
     CDS             complement(33754..34860)
                     /locus_tag="PC479_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2802970.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            complement(34974..35282)
                     /locus_tag="PC479_000382"
     CDS             complement(34974..35282)
                     /locus_tag="PC479_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2802971.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            complement(35285..35737)
                     /locus_tag="PC479_000383"
     CDS             complement(35285..35737)
                     /locus_tag="PC479_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2802972.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            35971..37899
                     /locus_tag="PC479_000384"
     CDS             35971..37899
                     /locus_tag="PC479_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2802973.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(38159..38920)
                     /locus_tag="PC479_000385"
     CDS             complement(38159..38920)
                     /locus_tag="PC479_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2802974.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            39139..39615
                     /locus_tag="PC479_000386"
     CDS             39139..39615
                     /locus_tag="PC479_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2802975.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            39713..39865
                     /locus_tag="PC479_000387"
     CDS             39713..39865
                     /locus_tag="PC479_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2802976.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(40025..40213)
                     /locus_tag="PC479_000388"
     CDS             complement(40025..40213)
                     /locus_tag="PC479_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2802977.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            40574..40924
                     /locus_tag="PC479_000389"
     CDS             40574..40924
                     /locus_tag="PC479_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2802978.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            40943..42586
                     /locus_tag="PC479_000390"
     CDS             40943..42586
                     /locus_tag="PC479_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2802979.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            42667..44724
                     /gene="recG"
                     /locus_tag="PC479_000391"
     CDS             42667..44724
                     /gene="recG"
                     /locus_tag="PC479_000391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2802980.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            44783..45337
                     /gene="rsmD"
                     /locus_tag="PC479_000392"
     CDS             44783..45337
                     /gene="rsmD"
                     /locus_tag="PC479_000392"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2802981.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            45339..45836
                     /gene="coaD"
                     /locus_tag="PC479_000393"
     CDS             45339..45836
                     /gene="coaD"
                     /locus_tag="PC479_000393"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2802982.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            45840..46364
                     /locus_tag="PC479_000394"
     CDS             45840..46364
                     /locus_tag="PC479_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802983.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            46708..48627
                     /locus_tag="PC479_000395"
     CDS             46708..48627
                     /locus_tag="PC479_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2802984.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            48640..48927
                     /locus_tag="PC479_000396"
     CDS             48640..48927
                     /locus_tag="PC479_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2802985.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            49060..50319
                     /locus_tag="PC479_000397"
     CDS             49060..50319
                     /locus_tag="PC479_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2802986.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            50322..51062
                     /locus_tag="PC479_000398"
     CDS             50322..51062
                     /locus_tag="PC479_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2802987.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            51385..52695
                     /locus_tag="PC479_000399"
     CDS             51385..52695
                     /locus_tag="PC479_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2802988.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            52793..53710
                     /locus_tag="PC479_000400"
     CDS             52793..53710
                     /locus_tag="PC479_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2802989.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            53726..54535
                     /locus_tag="PC479_000401"
     CDS             53726..54535
                     /locus_tag="PC479_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2802990.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            54593..56341
                     /locus_tag="PC479_000402"
     CDS             54593..56341
                     /locus_tag="PC479_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2802991.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            56603..57625
                     /locus_tag="PC479_000403"
     CDS             56603..57625
                     /locus_tag="PC479_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2802992.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEVSRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            57630..59936
                     /locus_tag="PC479_000404"
     CDS             57630..59936
                     /locus_tag="PC479_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2802993.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            59908..61023
                     /locus_tag="PC479_000405"
     CDS             59908..61023
                     /locus_tag="PC479_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802994.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            61041..62459
                     /locus_tag="PC479_000406"
     CDS             61041..62459
                     /locus_tag="PC479_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2802995.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            62474..63682
                     /locus_tag="PC479_000407"
     CDS             62474..63682
                     /locus_tag="PC479_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2802996.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(63756..65144)
                     /locus_tag="PC479_000408"
     CDS             complement(63756..65144)
                     /locus_tag="PC479_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2802997.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            65485..66819
                     /locus_tag="PC479_000409"
     CDS             65485..66819
                     /locus_tag="PC479_000409"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2802998.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(66961..67878)
                     /locus_tag="PC479_000410"
     CDS             complement(66961..67878)
                     /locus_tag="PC479_000410"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2802999.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            68086..68970
                     /locus_tag="PC479_000411"
     CDS             68086..68970
                     /locus_tag="PC479_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2803000.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            69183..71024
                     /locus_tag="PC479_000412"
     CDS             69183..71024
                     /locus_tag="PC479_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF2803001.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVED
                     IKIENKSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            71329..72036
                     /locus_tag="PC479_000413"
     CDS             71329..72036
                     /locus_tag="PC479_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803002.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            72030..73340
                     /locus_tag="PC479_000414"
     CDS             72030..73340
                     /locus_tag="PC479_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2803003.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            73457..74140
                     /locus_tag="PC479_000415"
     CDS             73457..74140
                     /locus_tag="PC479_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803004.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            74133..76520
                     /locus_tag="PC479_000416"
     CDS             74133..76520
                     /locus_tag="PC479_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2803005.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            76800..77942
                     /locus_tag="PC479_000417"
     CDS             76800..77942
                     /locus_tag="PC479_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2803006.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            77961..78485
                     /locus_tag="PC479_000418"
     CDS             77961..78485
                     /locus_tag="PC479_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2803007.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            78585..79403
                     /locus_tag="PC479_000419"
     CDS             78585..79403
                     /locus_tag="PC479_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2803008.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            79481..79792
                     /locus_tag="PC479_000420"
     CDS             79481..79792
                     /locus_tag="PC479_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2803009.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(79969..80652)
                     /locus_tag="PC479_000421"
     CDS             complement(79969..80652)
                     /locus_tag="PC479_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803010.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(80745..81386)
                     /locus_tag="PC479_000422"
     CDS             complement(80745..81386)
                     /locus_tag="PC479_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2803011.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(81560..83563)
                     /locus_tag="PC479_000423"
     CDS             complement(81560..83563)
                     /locus_tag="PC479_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2803012.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(83716..84354)
                     /gene="recX"
                     /locus_tag="PC479_000424"
     CDS             complement(83716..84354)
                     /gene="recX"
                     /locus_tag="PC479_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2803013.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        32700 a         9261 c        14459 g        27973 t
ORIGIN      
        1 gtattttgtt taaaaaagct tgaaaaattt gaaatataaa tatataattt gagttgatag
       61 attaaaaaat tatctttaaa cgatacagag atatcgtaag acaattgcta aattacactg
      121 tatatttttt atataagagc ttcttgtctt gtgatgagaa gtttttttgt tgtttaaatt
      181 gaaattttaa ataaaaataa atttataaaa aattaaaaga aaggagtaga tttgtcaaat
      241 agatatatta tatctttgac aataattatg atatggtaga aaaagctcaa ttaatggatg
      301 aaaaggcaat agcaagagca attactagaa taagtcatga aataattgaa agaaataaag
      361 gtgtagaaaa tctagtttta gttggaataa agactagagg tgtgccaatt gcaaatagaa
      421 tttctaagaa aatagaacaa atagaaggaa caaaagtaga tacaggagat attgatataa
      481 ctttgtatag agatgatttg gaaaaaattc atgttgaacc tgtagttaaa ggaacttatt
      541 tagattttaa tgtaaatgat aagacagtag ttttagttga tgatgtttta tacactggaa
      601 gaactgtgag agcttcacta gatgctatta tagatatagg aagacctaaa tcaatacaat
      661 tagcagtgct agtggacaga gggcatagag agttgcctat aagagcagat tatgtaggaa
      721 aaaatgtacc tacttctaga catgaaatta tatctgtcag tttattagaa attgatggag
      781 aagattcagt tacaatcaaa gaataattat ttttaagtat tatataaaag cttagcattg
      841 aatagtcagg gaggaggata aagcttacat agattgcaca aaataaatat tataatttta
      901 tggatgtgag tcttaatgta gtttttattc tcctttattt gagtttttga tgtcgtaaaa
      961 tgtaaaataa tgataaatta tataataagt atatagaggt taaaaaatag aatctaggga
     1021 atgataatta attaaaaggt attaattaaa cagaggattt atttatatat aataattttg
     1081 gaggttttta tgaaaaaaac gataatgtct ttacaacatc tattagccat gtttggagcg
     1141 acagtattag ttcccatttt gacaggtttt aatccgtcag ttgcaatttt ctgtgctggt
     1201 gttggtacat tgatatttca tttttgtaca gaaggaaaag tacctgcctt tttaggttca
     1261 agttttgcgt ttataccagt tatactagca gctaaagagg cgtatggtgg agatttagca
     1321 tatgctcaag gtggtataat agtagcagga ttaatttata ttataatgtc gattatagta
     1381 aaagtagttg gtgtaaataa aattaaatta tattttcctg cacaagttac aggagctatg
     1441 atagtagtta taggattaaa tttattgcca acagctttta gtatggcttc agccaatttt
     1501 gtgatagcat ttataacttt agcaatagca attttaacca ataagtttgg tagaggattt
     1561 ataaaacaat taggaatatt aatagcagtt ttttctgggt atataatctg tttgatatta
     1621 agattagtag atataactac tattacagaa gcaagtttat ttgcaatacc taactttaca
     1681 gttcctaagt tcagtttagg agcaatagtg attatatctc cagttgtatt agctgtattt
     1741 atggaacata taggagatat gactactaat ggtgctgtag taggtaaaaa ctttatagaa
     1801 aatcctggat taaatagaac attacttggg gatggatttg cgactatagt tgcagggtgt
     1861 ttaggaggac cagcaaatac aacttatgga gaaaatacag cagtacttgc tataactaaa
     1921 aactatgacc cttcaatact tagacgtaca gcaatatttg ctatattgct tgcatgtgta
     1981 ggcaagtttg gtggatttct tcaaagtata ccaggttcag ttatgggtgg aattagtata
     2041 atgctatttt cgatgataac ttatgtagga ttaaaaacta taagggacag ttcttgtgta
     2101 gaaagtaaaa taaatatatt aatcatagca gttatattat taataggtct tggaacaaca
     2161 tatttatcta ataaaggtat ttctgttgga atccctataa caagtacagt aaagataact
     2221 ggtcttagtt tagctgcaat tgttggaatt gtattaaaca gaattttaaa taatcaggac
     2281 tttaaagttg aagaagaata aatttaaaga ataagttgta tattgaataa ctaaaattag
     2341 ttaattttct atagacattt gtaattaatg ttgatataat gattaaaaat aaatgaaaga
     2401 aagggcaagg taactaggac aatgagaata taatctattt tttataattt aaagtattgt
     2461 aatttttgaa aagcctattt tggtggcttt atttgcaatg cttaaattaa ggagagtaga
     2521 ttatagtcta tacgtttatt tgttatgcct atttatagaa tctctcctta tatttaagtg
     2581 tgttaaggag ggatttttta tgttgttagt aaaagtagag aatttaaaga aatattatgc
     2641 agataaatta attttggata tagataaact tgaaatatta gaaaatgaca aaataggtct
     2701 agttggttca aatggtcaag gaaaaacaac attattaaaa gcaatattgg gagaaataga
     2761 aattgatgaa ggatatactt accttacaga aagctattct tatataagtc agagtgaaaa
     2821 taatattgaa acatgtagtc atagtaagga gaagagtctt ttaaatgctc cagataaatt
     2881 tgaagaacac ttatcaggag gggaaaaggt taagttaaaa atagcagatg cgctgagtaa
     2941 taagaaaaat atcataatag ctgatgagcc aacttcaaac ttagataaaa aaagcattgg
     3001 ggttttggaa gatatgttta aaaggcatga gggagcatta ttattaatat ctcatgatag
     3061 acgttttctg gatgagttat gtacaactat attagaacta gaagatggaa aactaaaagc
     3121 ttacaaaggc aattacactg attatttaat gcaaaaagat gaagaagtaa aaagagctga
     3181 ttttgagtat caagaatatg ttaaagagaa aaaaaggctt gaaaaagctc ttttatataa
     3241 aaaagcttta agtgatggca taagaaaaac accaaaaaga atgggtaatt cagaagctag
     3301 gttgcacaaa atgggtggtc agactaataa gaaaaagttg gactcaaatg tgaaggctat
     3361 aaaaagcaga attgataaac ttgaggtaaa gaataaacct aaagtttcta aagaaatgaa
     3421 cattaagatt caagatggta tggagataat tagtaaaaat ctagtagagg taaaagatat
     3481 gactctaaag ttagaaaata agctcctgtt agataatgtt tcctttaaga taaaaagagg
     3541 taaaaagata gcattattag gtgataatgg gtgtggaaaa agtactctga taaaagaaat
     3601 attggctgat aaaaatgaca atataaaaat aaataacaag gtaaaagtag gttattttga
     3661 ccaaaatcaa agtttattag atgaggaaaa gagtgttcta tacaatacta aagttaatag
     3721 ttcatttgat gaatctttta taaggataaa cttaagccta tttgggttta aaggtgatga
     3781 tgtctacaaa aaagtaaaag tacttagtgg tggagaaaag gtaaaaatag cactatgtaa
     3841 aataatattg gaagataata attttttagt atttgatgaa ccaacaaatt acttagatat
     3901 aaaatctatg gaagcactgg aaaaggcact gataaatact gataaaacta tgcttatagt
     3961 gtctcatgat agagtatttg tatcacatat ttgtaactat attatagaga taaaagatgc
     4021 taaaataagg gagtttgatt gtaattatga cgaatataca attagcagaa ataaaaaaac
     4081 tcctagtaga gaaaaccaaa tcaaaaaaga aaatcttcta gtattagaaa atagacttac
     4141 aactgttata tctatgttat ctatagaaaa agataatcta aaaaaagaat tgtatgaatc
     4201 agaatataat gaattactaa aacaaatcaa taaattaaaa aatagctttt agtgttaact
     4261 ttttaattac atctatataa gaagactgaa aatatttgtt gggaatataa gtatagttat
     4321 taaaacagaa aagtattttg ttagaaaatt aaaagtaagc taattagaaa gtggccttat
     4381 tattgtactt tataagaagg ccactatttt tatttagaat gataatgtgt agttgtctaa
     4441 catttatttt gtatttagtg cagaatttat tcagatttaa ccttaagctt ggctactgtt
     4501 tcttcttcag gagtaacata aatagtactg ttagtttcat aaataaccat acctggttta
     4561 gaaccactag gttttttcac attttttctc ttagtatagt ctacaggcac ctgagaagaa
     4621 agttttgact tactaaagaa tgctgctagc atagctcctt caaatacagt attatcaggg
     4681 acttccttac cagcacactt tattattaca tgagaaccag gaatattctt tgtatgcatc
     4741 cataagtcat cattatcagc aagtcttagt gtaagatagt cattttgctt attgttttta
     4801 ccaaccaata ttttaaatcc atcagaagaa acaaattcat gaggttttgt agatgggata
     4861 gtatcctttt tactgttttt cttttgagtc ttgatgtatc caaccttagc aagttcttct
     4921 tttatatctt gtaattcagc taaattttca cagttttcaa tacttaaaat tatgttttct
     4981 aagtaatcta tttcttcctt atttaaggaa atttgatgag atatctcgac tttagcatgt
     5041 ttcattttgt tatacttttt aaagtatttt tgagcatttt cagatggtgt caagttttta
     5101 tttaattcta ttgttacatc attacaattt tcatcataga agttagcaac ttcaatactt
     5161 tccatacctt tttctaccat ataaatatat gaagtaatca attctccttt tatcttataa
     5221 atgtctgcat tttcggattc tgataattct tcttcttgtt ttttaagttt attataaagt
     5281 ctatcaagct taacagatat actcttcttt aaatcagatg aacgttgatt aattctatct
     5341 ttaatatcct tagttctata gaaatcttcc agaatacgag acatactatc cttatttata
     5401 tatgataaat cgctaaataa tgtaaggttt atacaactga aatcaactac cttatccacc
     5461 tttttatcta taataatgca aggagagtat ttgttagaat ttatatcatt gaataaatta
     5521 caaaatactt tgtgtaaaga atcaaattgt tcatcagata tatcctttat gatagcattt
     5581 tgatttacac ctgctctgta gcaaatttct ttagctataa tagggcttat acctagaaat
     5641 ttagaatata tagacttaaa gataggtcct tccaattcct ctaggttttt aataaacaaa
     5701 tccttagata tatcatcaag agggttgagt ttgttttgtt ctggtggcaa tgaataatca
     5761 tgaccaggta aaacttgacg aactctactt atgctaaaag ggactctttt tatagaatct
     5821 ataattttat tttcttcacc atgagttatt attatgttgc tatgtcttcc cataatttca
     5881 atcataatat ttttaactgt cttttctttt aattcatcta aagattcaac acttattttt
     5941 attattcttt caaaacctat ttgagaaatt tctactatat ttccaccttg tatgtatttt
     6001 ctaagtaaca tacaaaacat aggcgctttt agagggtttt ctttttgata attacttgta
     6061 agataaactc taggatttga ggcacttgca cttaaaacca atctaaaatt ttctttatta
     6121 tttcttatat taaaaattac ctcatcatct tcaggttgat gtatcttatc tatttttcca
     6181 cctgtaagtt ttgatgataa ttcatcgact attgaatgta taactaatcc atctaatgcc
     6241 atttatagca cccctttctt tgaccaaaat gagtcagtaa attaattata cacataatat
     6301 acgctttgtg actaggggga atttaaacaa tagagattaa tatttaaatt gtataattat
     6361 ttatatttat tagagaggaa actttatttt taaaattaat aatttaaagt agattaaata
     6421 taaagtaaat atagtataat tattgtgtag agtataagct atgaaagtat atgaacttta
     6481 cagaaaaaat caattttaag gttctatagt atataaatat gaaagaaggt tgaaaaaatg
     6541 agatttatcg atttaagaag tgatactgtt actatgccta ctgacgaaat gagagaagct
     6601 atggcaaagg ctcctgttgg agattcaatc tttagagatg accctactgt aaataaactt
     6661 gaagaattag cagcagctaa agttggaaaa gaagatgcta ttttccttcc aagtggaact
     6721 tttgggaatc aattagctct atttactcat tgcctaagag ggcaagaaat aattattggt
     6781 aaaggttatc acattgtgac tcatgaggtt ggggcacctg ctgtaatagc tggagttcag
     6841 cttagaacta tagatgaaga cgaaagtgga gctctaaatc ctgaacttgt tgaaaaggca
     6901 attagaagag atgacataca tgaaccacag acagggctta tatgtgtaga aaatgcatat
     6961 tcaggtggaa cagttgtgag tcttgataat ttaagagaaa ttaaaaaaat agctgagaaa
     7021 cataacttac cagtacattt agatggagca agattattta acgcagcatt aactttgggt
     7081 gtagaagcta aagaaatagc aaagtgttgt gatagtgtta tgttctgtgt gtcaaaaggt
     7141 ttagctgcac caatgggttc tatacttgct ggaaataaag aatttatagc aaaagctaga
     7201 agaaagcaaa aagttatggg tggaggaatg agacaagtag gtatagttgc agcagctggt
     7261 ataattgcca ttgaaaaaat gactttaaga ttgaatgaag accatgaaaa tgcaaaatat
     7321 ttagcttgtg aattaaataa aattgatggc attgaagtaa acaatgttaa tccagatata
     7381 agtatggtat tttttaagat gagtgaagat attataaaag aggatgtttt aataaaagaa
     7441 ttttttgaaa gaaatataaa aattaataaa atggaaaatg gtgaatatag atttgtaact
     7501 catgtagata ttactaagga agatttagac tatgttttaa aaacacttaa agaattaata
     7561 ggtgtatgtt aatagatatc tactagaagg attatagcta gttaccagat gtttaattaa
     7621 aaaattcata gacagtttaa ataaaacata cataaaattc atatagaaat attaatatat
     7681 aaacatagaa agaactagct taaaaacata atataattac cctaattgta atggcaactc
     7741 tccctcacat cctcaaatta tagttgtcat tacaaaactt ttcccataaa ttataagttt
     7801 atgggaattt ttttatgtta taatagttta agtgttaatt tacgaaggag cgatacttat
     7861 gaaattttgg aaactacatg gaataggaaa tgattttata gcaatagatg gaagattcga
     7921 ccagataaat ccatctgatt atactgcttt agcaaaaaaa gtatgccata gaagattttc
     7981 tgtaggagcg gatggattgt tagttgtgaa agattcaaaa gttgcagatg tagaaatggt
     8041 atattataat tctgatggtt caagagcagc tatgtgtgga aatggcctta gatgtttttg
     8101 taaatttgtt tatgataatt gtatagttaa aaaagaaagt tttacagtag atacattaga
     8161 tggtataaaa gatataaaag taaatcttac taatagagaa ataaactcta taagagtcaa
     8221 tatgggaaaa ggaagtttta tagctaagga cgttcctgta cttacaaata aagaacgttt
     8281 tgtgcaagaa agtataaagg ttttagatag ggagcttaaa gttacttcta tgttgatggg
     8341 ggttcctcat acagtagtta tagtagatga attagatgta gatgatgttt gtaaatatgg
     8401 aaaagagatt gaaaataata aaatatttcc tgaaaaaacg aatgtaaatt ttgtaaaggt
     8461 agaagataag tataatatac atgtttatac ttgggaaaga ggttgtggat atactttagg
     8521 ttgtggtact ggtatgactg catcagcaat agtgtgcaat ttacttaata tggtagaaag
     8581 tagtgtaaat gtaacgtctc aaggtggaac agttaaaata gatgtaggag atttctctta
     8641 tatgacaggt ccagcagtaa aaatatgtga aggtattttg gaggtttaat taatggctat
     8701 aagtatgact ggttttggaa gaggagaata caaggatgat aattattact ttttagtaga
     8761 gtgtaaaacg ataaatcata agtattcaga tataaacata cgtcttccaa gaaaaatttc
     8821 atttttagag gataaagtta gaaatttagt aaaaaattat gtaaaaagag gaagagtaga
     8881 tttatacata aagtttgact tattaggcaa agaagatgta aacttaaatt ttgatgaggg
     8941 attagcatct cagtatatag acatactaaa agaaataaaa aataaatttg atattataga
     9001 tgatattagc gttatgaatg ttgcaaagtt tcctgatatt gtgaaaatag aagaaaagga
     9061 agaagatgaa gatttattgt ggtcaatgtt gaatcaagca gttgaagatg cattaatcaa
     9121 attaagagaa atgagaagtg aagaaggtaa aaaattagct gaagatattg ccatgagatg
     9181 tgatttatta aaaaatcata tagaagagat tgaaaaatat tcaagtagtg ttgtagaaga
     9241 ttatagagaa aaattgaatt tgagaataag tgaattactg gatgacccaa gtataataga
     9301 tgagaataga ttagcacaag aagttgcaat atatgcagat aaaagcagta taacagaaga
     9361 aatagttaga tttaaaagtc atatagggca acttaaaaat actatattta aagacgactc
     9421 tataggtaga aaaatagatt tcttaataca agagatgaat agagaaacta acactatagg
     9481 ttcgaagtca tctgatataa atataactaa tttagtagta gaagtaaaaa gcgaattgga
     9541 aaaaataaga gaacaaatac aaaatataga gtaagaatac aatattagaa ggggaatgct
     9601 taaatgtttg tgaaaaaagg attattgcta gttgtatcag gaccatcagg tgcaggaaaa
     9661 ggtactattt gcaaagaact acttaaagaa aatgatacaa taaaactttc tgtatcagct
     9721 actacaagaa aacccagaac tggagaagtt gatggagtaa attatttctt cataagtaag
     9781 gaaaagtttg aagagatgat agaaaaaggt gagtttttgg agtatgctca aatttatgat
     9841 aatttttatg gaactccaaa agcggcaata atggaatgtt tagaaaaagg acaagatgta
     9901 ttactggaga ttgaaatgca aggagcaaaa caaataaaag aagtttgccc agagggagta
     9961 tttatatttg tactaccacc atcacttaaa gaattaaaaa atagaattgt tggaagaggt
    10021 acagaaacag aagctgaaat agagaagaga tttagctgtg catatgaaga aattaaaatg
    10081 attaaggatt atgattattt tatatttaat gaagatgtaa aaacatctgc taaagaaata
    10141 gaaggtataa tatcatctga aaagaataaa gttagcagat ataaaaatat tataatagag
    10201 aaatttaagg aggaattata attatgttaa aaccatctat caatgaggta ctagaaaaaa
    10261 tagacaacag atactattta gtagggactg tttcaaagag agcaagaaaa ttaattgatg
    10321 gagaagaacc atatgtttca aataaaacta aggaaaagcc tgtatgtgta gcaactaaag
    10381 aagttgcaag tggaaaaata acatatagat tattgacaga agaggaaata gagatagaag
    10441 aagctagaca tcatgctgag caacaccaac aaatttcaga agaggagtaa ggtcatgcta
    10501 aaagataaaa ctgttgtaat tggcataagt ggcggtatag ctgcatataa ggcttgtgat
    10561 gttgtaagta agttaaaaaa actaaatgca aatatccatg ttataatgac aaaatcagca
    10621 acagaatttg ttaaaccact tacatttcaa tcgctgagtc aaaattatgt agtagaagat
    10681 atgtttgaag aaccaaaaac ttgggaagtt gaacatatat cattagcaaa aaaagcagat
    10741 ttattcttaa tagtgccagc aagtgcaaat gttataggca agattgcaaa tggtatagct
    10801 gacgatatgt tgacaactac tgttatggca acaaaagcac aggttcttat agcaccagct
    10861 atgaatacta atatgtatga aaatccaatt gttcaaaaaa atatacaaac tttaaagagc
    10921 tttggatata aatttattga accggaaagt ggaaggcttg catgtggaga tattggaaca
    10981 ggtaaactag ctaatccaga tataatagta gaggaagttt tgactaatct tactaaagag
    11041 caagatttaa aaggtcaaag tatgatagtt acagctggtc caactgtaga gagtatagac
    11101 ccagtaagat atttaactaa taggtcttct ggtaaaatgg gatattctat agcaagagaa
    11161 gctatagcta gaggtgctga tgtaacatta atatcaggtc ctacaaatct ttctattcca
    11221 caaaatctta aaaaatttgt tcaaatagag tcagcacaag atttgtatga agcagtagta
    11281 tcaaatttaa gtgaaaataa aatagtaatt caaagtgcag cagttgcaga ttataaacca
    11341 aaaacttatt ctgacaaaaa aattaaaaag aagaatgatg atttatcaat agaactaagt
    11401 agaaattatg atattgctta tgaaatagga aaaataaaag aagataagat actaattgga
    11461 tttgcagctg aaactaatga ccttatagaa catgccaaag gaaaaataaa aaagaaaaat
    11521 ttagatttta ttgtggcaaa tgatttaact aaagaaggtg ctggttttag agctgatact
    11581 aatattgtaa aaataataga tgtagaggga aatattactg agtatcctaa gatgaaaaaa
    11641 gaagaagtag ccaatgttat tttagataaa gttaaagcgt tgcttgagaa ataagacctt
    11701 ttttaaatat gatatttgtg tattttatca cagctaaagt ggctgaaagc tgcaatttaa
    11761 ttttagcgag taaaaactcg caaatgtcag ttaaattgta gttttcagca tttattttgt
    11821 cataaagtat aaaatataat taatagttaa ttattaaaat aaagttaata ttaataactt
    11881 gaaattgctt ataaattctg agattttaat atcttgtaaa gtatattaaa atatgaacaa
    11941 tatattaaat ttaagtagag gttttataat gaaaaaatat gcaaaggtaa tagttagaag
    12001 taatactata tatacagata atttatttac atatcaaatt cctgtatttt tatctgatgt
    12061 aataaaaatt gggcacagaa tattagtacc atttggaaaa ggtaataaac caacagaagc
    12121 ctttgtattt caatttacag atagcttaga tgaaaaaata aaaataaaag aaattattga
    12181 tatattagat gaaaatccga tatttagaaa agaagattta gagttggttt attggatgaa
    12241 aaatagatat ttgtgtactt atatagaatg tataaatttg atatatccaa agggatacaa
    12301 attaaataat tataaagtag tcctattagg tgaaagttta agtggattaa atgatattga
    12361 attgaaggaa aaaatatcaa ctttaagtga taaaaataga gaaatagtag agaaagtgat
    12421 tgatagtaaa ggtaaaatta aggttgataa gttaaaatat ataccaaact taaatagttc
    12481 tttgtacaca atgaataaga atggaattat aaaattatgt tgggaatata aaaatcacaa
    12541 aaatgaaaag aaggtatgtt atatatcttt gagtttagaa agtgataaga ttgacgatta
    12601 tatagagcaa aataaaataa atgtgggaag taaacaaaaa gagatattaa gttttcttaa
    12661 aaataatgaa aatgtagaga taaatgattt attggattta ttaaatgctt caaaacaaag
    12721 tataaattct ctaagtaaga aaaagctaat aaccctagaa tttaaggatt attatagaga
    12781 gccaaaaagt atttacaaat ccgtactaaa aagtataaaa ttaaacaatg agcaacaaga
    12841 agcagttgat gagataaagt cgaatatgtt tgtagatgat aaaaaaccat acttaattca
    12901 tggagtaact ggaagtggaa aaactgaagt ttacatggaa ataatagaat ttgctctaaa
    12961 tcaaggctta gacagtatat tcttagtacc tgagatagca ttaactcctc aaacaataga
    13021 taggctaaag agtaggtttg gagatttggt tggagtgttc cacagtaaac tatctgaagg
    13081 agaaaaacat gatgtttaca aagctgtcaa agcaggtaaa gtaagagtgt taattggagc
    13141 taggtcagct ttatttgcac cttttaattc cttaggctta ataataattg atgaatgtca
    13201 tgagtcatca tataagtctg agaagaatcc aaaatttaat gctatagaag ttgctagatt
    13261 tatggcatta aaaaataata taactcttat tttaggttca gcaacgccct caattgaaga
    13321 gtattatagg gcaaaatctg gagaatacaa acttataaat ataaaaagta gagcaaatga
    13381 taagcctctt ccaaacattg aagtggtaga catgaaagat gagttagata aaggaaatag
    13441 aagtatattt agtttgaaat tacaaaaaga aatacgttat gcgattgaag aaaataatca
    13501 agtaatacta tttttaaaca gaagaggtta tgcaaatttt gtatcttgta gaaagtgtgg
    13561 atatgtgttt caatgtgaaa attgtgatat atctttaact tatcataaga aaagcaatac
    13621 tggaagatgc cactattgtg gatatgagaa agaaattcca aaagagtgtc ctgaatgtaa
    13681 aagtacttat gttaagccat ttggagttgg aactcaaaaa attgaagagg aattaaaata
    13741 tatttttcca gatataaaaa ctttgagaat ggataaagat acaacttcaa aaaaaggtgc
    13801 tttagatgag atactaaata aatttaaaga taaagaagca gatgtactta ttggaacaca
    13861 gatgttaagt aaagggctgg attttgagaa tgtaacttta gtaggtattt tgtctgcaga
    13921 catgatatta aattttccag actttaaaag ttttgaaact acatttcaat tgataactca
    13981 agtctctgga agagcaggga gagctgataa agagggaaaa gtagtacttc agacttatga
    14041 cactgaacac tatgctatta agcatgctat tgaatatgat tatgagggtt tttatgaaga
    14101 tgagataaaa ataagaaaag catttggata ttctcctttt aataatatgt taagtgtagt
    14161 tgttagtggt gaagatgaaa gactagtgat aaaaaatata aaaaatatgc atgcatcatt
    14221 gatttattta ttagaaaaaa ggggtataaa tgatttagga tttatattag gtccaaatcc
    14281 atgttcaata tcaaaaataa atcaaaatta tagatggcaa atactcttta aagatgaaaa
    14341 tattgaaatt aatctcttaa agggtataat aaaatatata tgtataacaa aacgagattt
    14401 gatatttgat aagaatatca atgtatctat agatattaat ccaaacagtg tattataaat
    14461 ttaggaggaa ttataatggc tttaagacaa atagttcaaa taggagaacc agtattaaga
    14521 aaaaaatcaa aaaaggttga aaaaattgat gaaaagataa tacaattatt agatgatatg
    14581 gcagaaacta tgtatgatgc agatggtgtt ggactggctg cacctcaagt tggaatatta
    14641 aaaagagtag tagtaataga tattggagaa gagcttatag aacttataaa tcctgaaata
    14701 atagaaacat ctggggagca aatagatgag gaaggatgtt taagtgttgt tggagaagcg
    14761 ggaaatgtta gaagaccaaa ttatgtaaag gtcagagctt taaatagaaa tggtgaaaca
    14821 atagagttag agggagaaga acttttagct agagcatttt gtcatgagat agaccactta
    14881 gatggaattt tgtttgttga caaaatagaa aagtagtagg agttgatttg atgaaaatag
    14941 tatttatggg aactcctgat atagcagttc catgtttaca aaaaataata gatgagaagt
    15001 atgaaatatt gggtgttgta actcaaccag ataaaccaaa aggaagaggt aaaaaactag
    15061 gtatgagtcc agtaaaagaa ctggcaattg aaaataatat acctgtatat caaccagtaa
    15121 aggctagaga taaagaattt atagataaaa ttaaatcttt aaatccagat gtaatagtag
    15181 ttgtagcttt tggacagata cttccaaaag aaatattaga gattcctaaa cttggatgta
    15241 taaatgttca tgtttcttta cttccaaaat atagaggtgc ggcacctata aattgggtaa
    15301 taataaatgg tgaagaaaag actggtgtta caactatgta tatggatgaa gggctagata
    15361 ctggagatat gatactaaag acagaagtaa atcttgatga aaatataaca gcaggagaac
    15421 ttcatgataa aatgatgaat ataggcgcag aaactttaaa agaaacatta agattaattg
    15481 aagaaggaaa tgctccaaga gaagtacaaa atcatgaaga attttcctat gcgccaataa
    15541 tgaataaatc attaggtaac atagattttt caaaaagtgc cagagaaata cacaatttag
    15601 ttagaggagt aaatccatgg ccaagtgcct atactaccta taatgatgtc ataatgaaag
    15661 tttggaaaac taaagtgtta gatgaaaaaa gcacaaaaga tgtaggtaca ataattgatg
    15721 taagcaaaga tgggataaaa gtaagtacta tagataatgt acttttaatt gaagaaattc
    15781 agatgccgaa taaaaagaga atgttggttg gtgaatatat aaaagggaat actatagaaa
    15841 ctggtttagt attgggttag gtgaaaagta tgacagatat aaagaagtat ttaacaagtg
    15901 ttgggatttt gattttaata ttaggaattt tgataggagt tgttaatttt tttataataa
    15961 gtttaactca aatattctcc ttgatgataa atattataat aatatcacta atacttataa
    16021 tactatgttc aacagcagtt acttatagag taattaaagg aaagtatgtt aactcaaata
    16081 taatgaaaat aaattttaat attgtcagtg gattatttcc tattatatct ttcattgcat
    16141 catcatttgg tatgtcaaaa agtgatatta gaagaatata tataaagtta aataatgaat
    16201 atatatacag taataaatat aattttaatc cagaggatat aataatttta atacctcatt
    16261 gtattcaaga gaatagttgt aaattaaaag ttactaatga tatagataat tgtaaagaat
    16321 gtggaagatg caacatagga gaattaatta agctaaagga aaaaactaat gtaaaaatat
    16381 ttgtagctac tggaggaacc ctagctagaa aaattataat ggataataaa cctaaagctg
    16441 ttgtagctgt tgcatgtgaa agagatttga catctggaat acaagatatt aagaaaattc
    16501 ctgttttagg tgtatttaac aaaagaccta atggaccttg tgtaaatact aatgtagaca
    16561 ttatggatat tgaaaaagct ataggctttt taacaggaaa aaatatatta gtttgtaatt
    16621 aaactgtttt taagtgaagt gaatattaag aggatgtata ataaaaatat attttaggag
    16681 agtgttattt atgtaccctg tatatggcgg tttttgggga tttgacccaa caatggttgt
    16741 attaattcca gctatattac ttactatata tgcacaattt aaagtgagtt caacaacaaa
    16801 taagtattta agagtaaata cacgtcgagg atatactgga gagcaaacag caagaagagt
    16861 acttgattca aatggtttgt atgatgtaaa gatagaaatg gttagagggc atctaagtga
    16921 ccattatgac ccaagaagaa aggctgtaag gttgtcagaa gatgtatatt atggaacatc
    16981 tataacttct gttgcagtag ctgcacatga gtgtggtcat gccatacaac atgctaaagg
    17041 ttatgcacct cttcaaataa gaagtagttt agtaccagta gtgaattttg cttcaagtat
    17101 atcttggttt ttaatatttt taggatttat tatggctggt ccatttttaa aaattggtat
    17161 actattgttt tcggcttcag tgttattcca aataataaca ttgcctgtag aatttaatgc
    17221 ttcaagtaga gcaatcgttc aacttgggaa tttagggatt atagatgaaa gtgagtcaag
    17281 acaaagtaga agagtacttt cagcagcagc tttaacatat gttgcagcag cattggtttc
    17341 aatacttcaa ttacttagat tgttactaat agctcaaaga agaaatgact aaaagtaaag
    17401 tgggttgttt agaaacaacc cttttttatg tttaataaaa gggagaaatt tatatggatg
    17461 caagagaagt tggatttaaa gtattatgtg atatagaaaa aaatgacaat tattcaaata
    17521 ttgcaataaa taagcatttt aaaaatttag aaataagtga tatggataga ggacttgcaa
    17581 cagagttaat ttatggagta atagaaaata aatattattt agattatata atcaataaac
    17641 tttcaaaaat aaaggtaaaa aaaatgtcaa cttatgttaa aatcttttta agaatgggaa
    17701 catatcagat tttattttta gatagtatat cagattatgc agcagttaat gaaactgtaa
    17761 aattatctaa gaaatatgat aaaaaatctt ctggtttcat aaatgctatt cttagaaatg
    17821 aaataagagc taaagagact ataatggaca taactgagga agatagtgta aaatacttat
    17881 ctataaagta ttcctataac tcttggatta taaaaaactg gatagataaa tttggacaag
    17941 aatttgctga agatttgttg gaagcaaata atgaaaagcc tagtatctat ataagaacga
    18001 atacacttaa aataagtaga gaagaactta ttgaaaaatt aagtgaaatg ggtattgtat
    18061 gtttaaaagt tcctatggta gaagaagcta ttaaagtcga aaaaatgaaa aatatagaaa
    18121 aaaatgaatt atttaaggct ggtttattta caattcaaga tataagttct atgatagtag
    18181 ggaaagtaat taatccaaaa gaaggttcat ttgttttgga tgtatgtagt gctcctggag
    18241 ggaaatctac tcacttagct actttaatga acaatactgg tcaagtagta gctagagata
    18301 tttttgagca taaactaaag cttataaact ctactgtaaa tagattgggg cttaaaaatg
    18361 tgtttgtaga aagatttgat gcttctgaga ttgatgaaaa tagtataagt aaatttgatt
    18421 atgttttagc agatgttcct tgctctggtt ttggtataat aagacgtaaa ccagaaatta
    18481 agtataagaa agaagaagag ttaaaagaca tcacttctat acaaaagaaa attttagaaa
    18541 atgcatctaa atatgtcaaa gttggtggaa ctttagtata tagtacatgt acaattcaag
    18601 atgtggagaa tataaatata gttacttctt ttgttgaaga aaatcacaac tttgaatttg
    18661 ttccaattga tacagtaaat gtagacttag acaatcaaga taaaggatat ttaaagatat
    18721 atcctaatat tcatggtata gatggttttt tcatagcaaa attaaagaga ataaggtagt
    18781 gagagaatgg aaaagaaaaa gatagtatta aaaaatttta ctgaagatga acttaaagag
    18841 tttatgaaaa ctatagatga aaagcccttt agaggaagtc aaatattctc ttggatatat
    18901 aagggtgcaa aaacttttga tgatatgaac aatataccaa aaagtttgag aaataaatta
    18961 gaagaggttt catgtatagg gcatatagat atagaattaa agttagaatc taaggtagat
    19021 aatacaaaaa aatatttgtt tttattagat gatggaaata taatagaaac tgtgatgatg
    19081 gattatgata gtagagttac tgtctgtgtt tctaaccaag taggttgtag gatgggatgt
    19141 aatttttgtg catctacaat ggatggatta attagaaact tagagccatg ggaaatttta
    19201 gaccaagtta ttaaaattca agaagatact ggtaagaggg tatcaaatct tgtattgatg
    19261 gggagtggtg agccacttga taactttgaa aatacaaaac aatttttaaa aataattaac
    19321 gaaaaaaatg gtcttaatat agggtataga catataactc tttcaacttg tggaatagta
    19381 cctaaaatgt atgagttggc agacttagaa atagctataa atttagcttt atcacttcat
    19441 tcaccatatg atgaagaacg aagaaaaatt atgcctgtag caaatgctta ttctattgaa
    19501 gaaatattga atgcatgtag atattatata aaaaagacaa atagaagggt tacatttgaa
    19561 tattctctta taaaaggagt aaatgactca gaaaaagaag caaaggcatt agctaagctc
    19621 ctaaaaggta tgctatgtca tgtaaattta attccaataa ataaagttga agaaagagaa
    19681 tacgaaaaac cagataaagc atttatttat aaatttaggg atagtttgga gaagaacaat
    19741 atacctgcca cagttaggat gtctatgggt tctgatatta gtggagcttg tgggcaattg
    19801 agaagaaaat acaagtaatt tgtaaggagg aagtcgtatg gtttatagtt gtgcctccca
    19861 tataggaaaa ataagaaaaa acaatgaaga ctattgtgag ggagaagtta tagatacaga
    19921 acatggtcca ataggaatat ttgccatagc tgatggaatg ggtggacata aaaaaggtga
    19981 agtcgccagt aaattagcag tagaaaatat aattgatttt ctaaaagaaa acttattaca
    20041 acatgataat gtaaaaatag actatataga tgatatatta aaacaggctt ataataatgt
    20101 aaactcaatt gtgcacaaaa aatctatgga agatatagaa tttgaaggaa tgggaactac
    20161 attagttacg gctatagtgt ataataatgt cttatatgtg gcaaatgttg gagatagcag
    20221 atgttatttg ttaacagatg aaaaatttga taaaataact atagaccatt ccgtggttga
    20281 agaacttatg atggcccatg tcattacaga agaagaggca agaagacatc cacagaggaa
    20341 tagaataact agagcaattg gaacagatga tatggttgtt gtagatattt ttaagaaaga
    20401 aatcaaaaaa agtgatataa tacttcttgc gacagatgga ttaactgggt ttattgatga
    20461 tgaagatata agagatttaa tattagatta tgaatatgag agaactagta atataagtga
    20521 agaattgata agtatggcca atgatgtttc tggtaaagat aatgtttcgg ttatagtgat
    20581 aaaagtataa ttaggacggt gatattgtgg gagatacaat tttaggaaat cgatatgaaa
    20641 tcatcaggaa gattggtgat ggaggaatgg cctttgtata tgaggctaaa gacagattat
    20701 taaacaggac tgttgcactt aaagtactta gacctgaatt tgtagatgat gatgaatttc
    20761 taacaaagtt taaaagagag gcagaagcag tagcaagtct ttcacatcca aatatagtaa
    20821 atgtttatga tgttggtgaa gatggaaaag ttcactacat tgtaatggag tttgtagatg
    20881 ggaagaatct aaaagagatt atacaagatg aaggtatatt agatgaatat actgcattag
    20941 atataacaaa acaaatagct atggcactta gtgcagcgca caaaaagggg attatacata
    21001 gagatataaa acctcataat attctcatat caaatgaagg aagagtagta aaagtggctg
    21061 attttggtat agccaaagct gtttcaaatt caacaatgac taatattggt agtataatag
    21121 gttcagtgca ttatttttca ccagagcaag ctaaaggtaa atttgtaact aataatgctg
    21181 acctgtattc tctaggtata gttttatatg aaatgttgat aggaaaagtt ccatttaggg
    21241 gagatagtcc aatttctata gcacttcaac acattaatga tgatatagac tttacatcag
    21301 aagaaaaagt taggattcct caaagtgtaa gaactacaat aaaaaagctt acagaaaaat
    21361 ctagtgcaga tagatatcaa actgctgaag aattgataga agatatagag tatatagaaa
    21421 aaaatattga tttagatttt ataaaagaat atgatgattt tgcaactaag aaaattgatg
    21481 aaaaagaaat taataaagtt gtaaatccaa cacttgcaaa accagctcct gaaaaagtgg
    21541 ttaagcctgt tgaagtagct gatttggatg atgatgaaga ttactatgat gatttttatg
    21601 aagaagatga tgatgaagat gaagaagagg aagagataat gagagctaaa aagaatcaga
    21661 gacctaagag tactccaagt aagagaacta agaaaaagaa gaaaaaacag gaaagcccaa
    21721 aatctagaag aagattaaaa gtaattgcgg ctgttttgat actgatattg tgtgctcaag
    21781 tattcttagc atataagttt ttatttgctg gagggtttgg caataaatct ttgactgttc
    21841 ctaatcttgt aaatatgaca ttagaagaag ctcaaagtgc tgttgaaaaa gaaggattat
    21901 acctgagtgt aaaaagtgaa gagtataata gtgaagttga tgaaaattgt ataatatcac
    21961 agacacctga aggtggaagt acaaatgtca aaaagggtga tactataaat gttgtcgtaa
    22021 gtaaaggttc aagtcaggca tctgttccta atgtagtggg acttactttg agtaatgcaa
    22081 aacaacttat tgaagaaaat aacttaaaag tgggaactgt taagtatgaa tacagctcaa
    22141 tatataaaga aggtacagtt ttaagtcaga gtccgggtgc tggctcatca agagctcagg
    22201 aaggtgatga ggtaaaccta tatgtaagca aaggttcaga aaaatcaaat acacagactc
    22261 caacagtacc agataaaaaa ccaacaacac cagagaataa tacaccaaca gaacctggtt
    22321 caaactcagg aaacagtggt ggaaattcag gaggctcaaa ttcaggaaat agtggtggaa
    22381 actcaggcaa taatggaagt aactcaggag gttcaaactc aggaaatagt ggtggaaact
    22441 caggaggctc aaactcagga gataatggag gaagttcagg aggctcaaac tcaggagaca
    22501 atggaggaag ttcaggaggt tcaaactcag gagacaatgg agatagttca ggaggctcaa
    22561 actcaggaga taatggagga acttcatcag gaactggcgc aaacattggt aaaacagaat
    22621 aaatattaat aaaataagag ctgtcctttt atagaaaata ttttctttaa agggcagttt
    22681 gttttgtata aaatactgaa ttggttataa tataaggaga aaagtattta tgaagaagaa
    22741 aacagtttta attacaggag gagctagagg gattggtaaa gctatgtcta aagcttttgc
    22801 aaaagaaggt tacaatgtat tagtaaattt taataagtca gaaaatgaag ctaaagagtt
    22861 gtacactata ttaaatgaaa aaaatttttc tgtaaaactt tttaaggctg atatttcaaa
    22921 tagagaagat gtagaggata tggtagatta ttgtataaaa gaatttggag gattggatgt
    22981 acttgtaaac aatgctggtg taagtcaaga taagttgttt acagatataa ctgatgaaga
    23041 ttgggataat atgatgaata ttaatttaaa aggaagcttt tattgttcac aagttgcttt
    23101 aaaatacatg atatcagaaa aaaaaggaaa tataattaat atatcttcaa tctggggtat
    23161 atcaggtgcg tcttgtgaag tgcattattc tataacaaaa gcaggcataa taggtatgac
    23221 aaaggcatta gctaaagaag taggaccatc aaatattaga gtaaatagca tagctccagg
    23281 tgtcattaat actgacatgt tatctggata taatgaggaa gatatagacg ctcttgtgga
    23341 agaaacccct ctaatgaggc taggaactcc agaagatata gcaaattgtg ctattttctt
    23401 ggcatcagac aaatcaaatt ttatcactgg tcaggtaatt agtccaaacg gaggatttgt
    23461 tatttaaaaa aaatgtggta ttatataaaa ataggaaaga ataacacgat atactactca
    23521 taaacagtat gtgtttatat aaattttata aagggaggca tttgatgcta gaaggaaaaa
    23581 ttataaaggg gattagtgga ttttattatg tagatactta taatggtatt tatgaatgta
    23641 aggctagagg gatatttaga aagcagaaaa taacaccttt ggttggtgat agagtcaaaa
    23701 taagcattgt tgatgaaaat gaaaaaaagg gtatcttaga agaaatcgac agtagagata
    23761 cagaacttat aagacctcct attgcaaatg tagataaagc attaatagta ttcgcaataa
    23821 aaaatcctaa accaaactta tctcttttag atagatttat agttttggca gaaaaagaaa
    23881 atttagaaac tgtaataatc cttacaaaag cagatttaga tgataatgat acattagaaa
    23941 ctgttaaaaa tatatatgaa cttagtggct ataaagtcat tcctgtaagc aatattacta
    24001 agttaaatat agataaagta aaagaagaat taaaagaaaa tgtagtagtg tttgcaggac
    24061 catctggagt tggaaaatca agcttattaa atgaaataga tgaaaatttt aaacttcaaa
    24121 caggagttgt aagtgataaa ataaaaaggg gaaaacatac tactcgtcac gctgaacttt
    24181 taaaattaga atttggtgga atggttgcag atacaccagg ttttagttcc cttgcccttg
    24241 aagatataga ggaagttgaa cttaaagatt attttataga atttgataaa tttaatgatt
    24301 gtaagtttgg ttcaaagtgt attcatgaaa atgaacctaa ttgtgctata aaagaagctg
    24361 ttacaaatgg agaaatatct aaagaaagat atgatagtta tatacagctg ctacatgaaa
    24421 taagacaaaa taatagtagg aggtattaga aacatgatta aattagcacc atcaatatta
    24481 tcagcagatt ttgcaaaatt attagaagat gtaagaaaag tagaaagtgc aggatgtgaa
    24541 tatcttcata tcgatgtaat ggatggacac tttgtaccaa acattacatt aggaccactt
    24601 gtagtcaaaa gtttaaagaa agaaaatata aatatggtat ttgatgctca tcttatgata
    24661 gaaaatccag accagtatat tgaagaattt gtaaaagcag gatgtgatat aataactgtt
    24721 caccaggagg cttgtgttca tctacataga actatacaaa atataaaatc acatggaata
    24781 aaagctggtg tagtacttaa tccagctaca ccagtagata cgattaaaca tgtactacct
    24841 gatttagata tggtattatt aatgtctgtt aaccctggat ttggaggaca atcatttatt
    24901 ccttgtgttt tagataagat taaagagtta aaagctatta tagatagtca aggattaaac
    24961 attgatatag aagttgatgg tggtataagc cctaaaaacg tagccgaagt tgtacaagca
    25021 ggagctaatg taatagtagc aggttctgca atatttggaa gtgatgacat tcaagaaaca
    25081 gttaatttat ttagaaagaa tgcttctctt gaagaattag tttaaattta ataatgaaaa
    25141 tctgtattgt tttaaatggc gaaattgaag attacgaagt tacaagagat attataatta
    25201 aggaatgtta tgactgcata atatgtgcag atggaggggc aaatcacact tataaaatgg
    25261 aaataatgcc agattatata ttaggagatt tagattcagt agaagaagaa aagataaact
    25321 tctataaaaa taaaggtgtt aaatttgaaa aatttccatc taaaaaagat gagactgata
    25381 cagagctttg tcttttttta gctaagacac ttaaagcaaa tcatattgat ttttttggtg
    25441 ctttaggggg aaggattgac cacactcttg caaatataaa gctattatat tatttaaaag
    25501 aagatggtat atattctagg atactttctg ataaagaaga gatgtatata gttgaaaatg
    25561 aggaaatttc attgtatgga aacccagggg acactatttc tgtaatagct ataaatggtg
    25621 atgctaaggg tgtaacactt actggcttag aataccctct cgataattat tatatgaaat
    25681 actctgtacc tattggaata tcaaatgtta tgttgagtaa ttcttgtaag attaaagtag
    25741 agcaaggctg tgtacttgta gttagaaatt tataaaattt gattttgaca attggtttta
    25801 tattgtagag cagatacaat caagtaaaat atatttatga ttgtatctgc tttttagtat
    25861 ataattataa atatataata aactttgaca taagggggat tttttgtggt agatagtatt
    25921 atttttgatt tagatggaac attgtgggat tcaactgaag aagtatgcaa agtctggcag
    25981 gatgttttaa gtgagcataa ggaaatagaa ttaagtgtca caaaagattt atttagaagt
    26041 cttatggggc tttcttttga ggaaatagca aatcgtttat ttccaaattt aagtgaagaa
    26101 gaaagaatga atttattgaa tgaatgttct ttgaaagaat gtgactattt atctgaaaat
    26161 ggagctaaat tatttgatga tatagaagat actcttaaat tactatctaa aaaatataaa
    26221 ttatttatag taagtaattg tcaagctgga tatatagaat cttttttaaa agcacataaa
    26281 ctagaacagt attttataga ttttgaatgt cctggtaata ctggtttgca taaaggagaa
    26341 aataataaac ttataattga aagaaataaa ttaataaatc caatctatgt tggtgatacg
    26401 caaggtgatg caaatagtgc taagtttgta ggtatacctt ttatatatgc caaatatggg
    26461 tttgggaatg tagatgtata tgattatttt atagattcat ttaaagattt gttagaacat
    26521 gatatattaa aataagatga gtttaaaaat tatattaaaa taataaaaaa gttatggggg
    26581 gatttaaaaa tggaattaca agatactatt tttaaaagac aaagtgtaag aaagtttaaa
    26641 aatcaagatg tatcagatga agatatttta aaaatgataa aagcagcagg tgcagcacca
    26701 tcaggcaaga atattcaaaa ctggcatttt gttgttataa aacgccgtga tttaatggaa
    26761 aagatagcag atgtgataac taaaaagcaa caagaaatac ttgtagaaat ggataaagta
    26821 tcagtggata aggctaatag atttagaaaa tttgttaaaa attttacact attttactta
    26881 aaagctccag ttttagtatt agtttttaca aaggtatata atccatcagg atattatgaa
    26941 ttagagctta tagatgcacc taaggagact atagataaat tatttataag aaatccaggt
    27001 atgcagagtc taggtgctgc aattgaaaac tttacactat cagcaattga acttggatat
    27061 ggttcttgtt ggttgacaag tcaaaactat gcagcagatg agatagaagc tgttttagaa
    27121 gcagaaactg gttttgaaaa gggagagtat tttttaggtg caatgttagc tcttggagtg
    27181 ccagaggaca atttgaagag tccatctaaa aaaccagtag aagaaatatg tacatttata
    27241 aaataaaagt caaggacatt ttaaaatcga agtaaattaa aaacaaaata aattaaaaat
    27301 aaatatttaa aaaattaaaa agtaggtttt attgatatta attttatatt tcaaatcatt
    27361 gatttaatta atacaataag aactactttt tttgtatatt aattttaaat atatttaaat
    27421 ctttagaaaa tctttagaaa agtatatgta atatcttaag tttttaattt taatattaag
    27481 tatataaaac aaaatgaaaa aatattaaat ttaaactaga aggaggtttc taagatggac
    27541 attaaataca aataaccaga acaaaagata taaatatata tataaatgga aaaacttcta
    27601 tcatattgta taatcatata tgtaaagaaa atttataaat taatataaag gagattagaa
    27661 tgaattcata taatattttg gtggtagaag atgaaaagga gatagcagat gctatagaaa
    27721 tatatctatt aaatcaagga tataatgttt ttaagggata caatggatta gaaggtctta
    27781 aggttataga aaaccaagaa atacatttag ctatagttga tataatgatg cctaaaatgg
    27841 atggtataac acttacaatg aagcttagag aaaactataa ttttccagta ataatgctat
    27901 ctgcaaaatc agaggaagtt gacaaaataa tggggcttaa tataggagca gacgattatg
    27961 taacaaaacc ttttaagcca ttagaactat tggctagagt taattcacaa cttagaaggt
    28021 acactaagta tctaaacatg gttggaaata aagagcaaaa ggtagaaagt gacgatagtg
    28081 tgtttgcaat aggcggatta gaattaaatg aaaatacaaa agaagttagt attgatggaa
    28141 agtatataaa aacaactcct atagaattta aaatactaag tttacttatg agaaatgcag
    28201 gaagagtttt ttctgctgat gaaatatatg aaagagtttg gaatgagaat gctgtaaata
    28261 cagatacagt tatggtacat gtgaggaata taagggaaaa gatagaaatt gacccaaaaa
    28321 atcctaaata tttaaaggtg gtgtggggtg ttggatacaa aattgaaaaa atttaaagat
    28381 aaatatatag ttactattat agtttttata cttatgttat cagcatcatt aggtatgatt
    28441 agtcaatatt ctactataca agcaagtgct aaaggtggag cagagaatcc atttgaacag
    28501 gaaagctttg tagatagtat atataaagga agttatgttt tatatcataa tatgaaggaa
    28561 gaacaagaag gtaagatgat acaaccatca aaactattta tcaatgaaga aactatgaac
    28621 tatataaaaa atgtaagtaa agaaaaagat acttatgatg gttcagaaac ttactataca
    28681 gatgatagta ttaatgcaga ttttaatgaa aactttgaaa attgggagca tttcattaaa
    28741 gcgtcaggaa agaatttaaa gtactattta gttgataaag aaagcaattt tgaatttttt
    28801 aatgataata aagaattagg agtacttgat acaacaaaaa atgatgcttc aaaagaaaat
    28861 tctgatgaaa atcagtcaaa aaatacagaa atgaatgatg aggaaagaca aaagcagatt
    28921 acagatataa aagaaaacta tagattctac gttgtaatga attttgataa agatggaaaa
    28981 gtaaatattg tggattctta tggagtaaat caaaaaatta ttaatcaaat gttgttatct
    29041 aaagcaagag aagatttaat ggatacaagt ttaggaggaa attcaatttc atataagtta
    29101 tctccaataa aaaatataac attagtatat gctgtaccta aagtaataag taaaggtgat
    29161 agtgtagagt atggttatgg aaatacttat tttgatgatg gtataatttc atatataagc
    29221 aatgtagaaa ataattctta ttataagatt tctggaatga ttttaaaaat tatattagct
    29281 atagttgtta ttgtagcttt agcattccca tatagaaaat ctaaagagtt gctaggattt
    29341 gatacattat caagaatacc gttagagata ctctttattg ctcttgctat tatacagggg
    29401 cttgtagatt tatatcctac tgttataata agtaatacct tgagtgggaa ttatgtagag
    29461 taccttataa acaatgacat aagtgctaaa atggctagta tattggtaag tttaatgaat
    29521 ttaacttatt ggtttgtaat attctcaata atatttgtat ttataatact tttaaaacat
    29581 attttaaatg ttggaataag agagtatttt acaaaacata ctgtgacagg aatgtttttt
    29641 gtatggctaa aaaaagcagg aaaaacagtt attaatcaaa ttagaacaat aaatctaaaa
    29701 gaaaagccaa ataaaactat agttacaatt ttgggaataa atttattgat tatagttata
    29761 atgtgtagta tgtggttctt tggaataata ctcgcattaa tatattcagt agtattattt
    29821 aaaatactgt cagattactc taaaaagaca atatatgaat ataatcaact tttagatgtt
    29881 acaaagaaaa tttcagatgg aaacttagat gtaaatatgg aaaaagattt aggatttttt
    29941 aatcctataa aagacgaatt aggaaatatt caaactggat ttaaaaaagc tgttgacgaa
    30001 gaggttaaga gtcaaaagat gaaaacagag cttatatcaa atgtatcaca tgacttaaaa
    30061 acacctctaa catctataat cacttatata gatttattaa aagatgaaag tataacagat
    30121 gaaaatcgta agttgtatat tgatacatta gacagaaagt cacaaagatt gcaacatcta
    30181 atagaagatt tgtttgaagt aagcaaggca aatagtggag atgtttattt gaatatagta
    30241 aatgtggata ttgtatctct aatgaagcag acacttttag aattagatga caaacttgca
    30301 gattcatctc taatagtaaa gaataacttt tctaatgaaa aaataatatt accattagat
    30361 agtcagcgta catttagagt atttgaaaat cttataataa atataagcaa atatgcaatg
    30421 ccaaattcaa gagtgtatat tgatatttta gaaacagata ataaagtaaa tattatgctt
    30481 aaaaatatgt ctgcatctga gattgatttt agtgtagatg atataatgga gagatttgta
    30541 agaggagata aatcacgtaa cacagaaggt tctggtcttg gtttagcgat agcaaaaagt
    30601 tttatagaat tacagggtgg taagatgaat ataagtgtgg atggtgattt atttaaagtt
    30661 acaattactt ttaacaaaaa ttagattaga cttctttaat aattataaag tctataggat
    30721 aaaagagtct ggaaatattt atgtgtattc tttaaataaa aatttatagg tattgtttta
    30781 aaataatttg taagcattat ttcaaaacag cttataaatg ttattttaaa acagaatgtt
    30841 gatttattta aaaaaggtga atcctaatat acaaggttca cctttttatt ttacaaaatg
    30901 ttatttagtg ttacagagct tataaatagc ataatatctg gctttactat taacatggta
    30961 ctagcaattt attttgaaat cttgtataag ctattttata ttatttaatt actattgttt
    31021 gtactttaca atgtgataaa gaatttacct ctacagtttt agtatttact ttaagcttgc
    31081 tagtattatc atagatttcg tcgagcattg cactataagc atcattgaca tttttattga
    31141 tgttaagact tcccttagca tcctcatttt tcataccatt gaagattcta actacaattc
    31201 catctccttt ttcttctttc tttattgcac ttactgtgaa atttgaatta tttacattca
    31261 tcaaactaaa ttcattttca tatatttgtt tttcgtcatt gaatgcaaat ataagtctac
    31321 catttaaaaa atcagcatat tcatatattt gtataggtgt taagaatctc ttagctgtat
    31381 tagctacatt tgcttcatca aatttattgt tatatatgta aagaccaaag tcaaagttta
    31441 actctttaag taattgtgca tctggtgttg ctattatttt ttctccagaa gctcttcctg
    31501 gtctataaag taaattttct cttcccatat atccaaatga cctgaatagt gttaatgcta
    31561 ttgtatctaa attatcacca actatttgat attctcttac acaaccagtt attaatgcta
    31621 ttcctctttg tccatcctct aaagtaacaa aactttgcat tggttcgata gttataggtt
    31681 tttcttgcca tgagtaattg tctcttttcc acaatgcaag gtctttttct aatactgttg
    31741 gtctttgtat tattccaaat tgttgatctg ctatagaaaa cttagatgct atttctgtat
    31801 taaataaaac tcttactcta tgagatagtg cttggttatt ttttatatta acattaaact
    31861 taattatttg ttctttaggt tctaaggtga ttttcattgt tactggcatt ttcacagaaa
    31921 ctttacctat agccctttct tccagtgaag ctggtatggt caatgaatat ttaagtatca
    31981 tttcttcttg aatgtttgat tttagcactt ctactgaaga tacagaatct aaagatgaaa
    32041 cataaatatc ttttcttgga ggagaatagt tgtatgaatc tccatcatct ccattgtctt
    32101 caattacagc ttggttttta taagtcttat tagattcttt atctaatatt tctaatgaac
    32161 catttttatt ggctgttatt ttgtagtatt tgttttctat ttccttgcaa ttacttgttt
    32221 ctatttcaca tttattttta gtatccaatt caaaaactag ttgagtatat cccattggtg
    32281 gtatatcttt tactgaaagt gcaatttttg ctctatatac agttttgggt atatatattt
    32341 ttttacttgg gtttaatctt attgtttgag ttaatatata atttgtaata tcttcttttt
    32401 cttttattat ataattaaga atttttccat ttttattctt tatcacaaaa tcttgacctg
    32461 gaatataagt atcaaattct acaacttcat ttctgacttc tgggaatcca ttgaatactg
    32521 ttaatgttat ttcttcatta ttatttttga tttgagtact tataagtctc atatgcaaat
    32581 ctaataaatt tatactcaaa tctctggctt gtttgtatct tgtgtataca tcctcatttg
    32641 ttgtgtcact attacaagaa cctatgctgt catgagctgc attttcaaac ataagtttcc
    32701 atatatcttt tataactagg tgagggtatt catttccaag tgaataactt aatgttaata
    32761 acggttcaag tacatttgta atataatttt ctaatttgtt attcattatt ttaaggtctg
    32821 accttgagga gaatatggat ttgtgtattc tcatatgttt agcatttaaa aattcacctt
    32881 taagctctgt aaattcattt ctttcatttc ttactgaatc tatatagttt tctatatcac
    32941 ttatttcgta ttcattttca ttatctattt cattagcttt ttttaatata tcaggaaggt
    33001 tttttcttat tggtgcttgg tcaaatccat ttggaaaata tacgttatta gtagaagctt
    33061 taaccttaag ttttacgatt atatctttta aatattcttt taaatctgaa tcatcttctg
    33121 gtatattacc tccatagtaa taaccaaatg gtatttgaac tgctaatact tttgaaccat
    33181 cagaaccttt ccatataaac tctgttgtat ctaccatatc atcagaaact ccacgccaaa
    33241 ataatgaact atctattcca aaacctctgt atatttgtgg catttgagct gattgcccaa
    33301 aagaatctgg tacatatcca accctcatag gttcaccaaa ttcattgcat ctatctattc
    33361 catagtaaag gtttctaatt atagattctc ctgatataac caattggtct gtttgagtat
    33421 accaaggtcc aataactaat cttcttcttt gaactaaatc tcttattcta tatttatcct
    33481 gaggtctcca ttttaaataa tcatctaaga gagaagcttg tgcatccata gtaaagtatt
    33541 taaagtcttc tttttcttcc aatatgtcta atatatcttg aaaatctttc attaaatata
    33601 tttttgacct tgatgttgta aaataccatt ctctatccca atgactgtgt ggaactacat
    33661 gtacttttgt ctttttcata aaaatcctcc caaaatttaa ttgagaattg agagtacact
    33721 caattctcaa ttatctaaaa ttaagttttt aatttacatt atatctaact ctatttcatc
    33781 aaattctgta ttttccgtag tttcatcttc attgaagtct actaaagttg ttgataatat
    33841 tgctattact aaagctccaa atattgcacc tactaagtaa gcccatccag aacctattga
    33901 tataaatcca tataatccac caactggagg cattatagca tgtacaccta acaaagctga
    33961 taatcctaca cccaaagcgc atcctatcat attaacagga actaatttta atggatttgt
    34021 taaagtgaaa ggaattgcac cttctgttat tccaattata cccataacta tattgccttt
    34081 tcctgtttca gctaatgcat tggaaaattt tgattttctt atgaatgtag ctaaaccata
    34141 tcctagtgga ggtataacta ttgcgcagtt taccaatatc gctggtagat agattccctc
    34201 taacaataat gcatttcctg ccatccaagc tgctttattt actggtccac ctaaatcgaa
    34261 tcctatcatt gcgcctaaaa ttaaagccaa taccaccgaa tttgttccat tttgacacat
    34321 ttcagctacc caagcattta atgaagtatt tatgtttcct aaaggtcctg ctaatacaac
    34381 actcattaaa aaaccagtta atccaactgt tataagtggc attattataa gtggcatcat
    34441 tcctgatgct gacccagata ttttaatttt tttctttaaa aacttacaaa catacccagc
    34501 aaagaaacct gctattactg ctcctaagaa tcctgcatta gtattacttg ctatgagccc
    34561 accaataaaa cctgcaccca tagctggttt ctcacctatt gaaaatgcta taaatcctgc
    34621 taataccgta tttaatagac ctatgccagt ccaaccaact tgttccatta agtaaagaat
    34681 atgataaaaa ccagatacat ttttataagc atctaaactt gttataccca ctgctaaagc
    34741 tgtaagcttt gggatagcta caactaaaga tgagcctatt acaagtggca acatatatga
    34801 gatacctgtc attaagtgtc ccttaagttc acttaaaaga gatttatttt ttttactcat
    34861 ttttaatcct cctatataag tttgttatat tttttgataa tatgttataa ttacattaga
    34921 caatttttta atttttgatt tttctcattc tatattagag tgagattttt ttattaagaa
    34981 atagcttcta tacactttgt taagacagct tctggttttt ttaacgctat acctattttg
    35041 acttttatca cttttttacc atcaaatctt tccattccag atatagtttg gtcagatgct
    35101 ataagtataa agtccgcatc ttgtgcttct tcttttgtta tagtatttac tcgtcccata
    35161 gcaccttgtt gttctacttt gatttcatga cccatttttg atgcagcttg ttctaaagct
    35221 tttgctgcca taggtgtgtg tgctaatcct gctatacatg atgttatagc tactattttc
    35281 ataattattc ctccttcatt ttattagtta tatattttac taaatgactt gcatcactag
    35341 catttttaac ttcacttgta aaatcatcct ccattaaaca tgtggctaat ttagatagag
    35401 ttttcaaatg tatatttcct tcatttttat gtggggctaa caaactaaat acatatctaa
    35461 ccttactatc atctaaagtt ttccattcta actcgttagt tgttttaata tagagtatgg
    35521 aagcttcctt tacatttaca ctctttgtat gaggtatcgc aaatccatct tttattcctg
    35581 ttggaaattc attttctctt tttagaaggt cttttaaaag agcctcttta ttagttgtaa
    35641 tacctagtat ataagcacta tctgcaataa aatttaatac ttcttctttg ctttttaagt
    35701 ctttatctat aaatatatat ttttcttgta gtaccatata tttttccact ccattcataa
    35761 tgtcttaact tatatttata ttgtaatcat tatcctaatt aaattaaata taactaattt
    35821 ccacttcgac atggaaattt atattatgat aataactaat ttccatatag aagtggaaat
    35881 aaattattat aatacaattt tattagagta attgtattat atgagtgtaa atagtataat
    35941 ttagaagaaa ttctataagg ggggatattt atgcacaaga gattgctcac gttgtttaaa
    36001 ttattaaatg aaagtgatga taaaattact tgtaaaactt taagtaatca tttaaaagtg
    36061 agtgagagaa ctataagaaa tgatattact tctataaatg gaactttaga gaaaaatggt
    36121 gctattatta aaataaaaaa aggagaagga tattatatag atattcttaa tttagcatta
    36181 taccaacagt atctagcatt aatatctgat gatattatgg attcaagtga aataccagat
    36241 tctcctatag aaagaaatca atatatttta aaatatattc tctataacaa tacatatata
    36301 aaactagaag atttagctaa tagcttgtat gtgagtaaat ttacaatact aaatgacata
    36361 aaaagaataa agcctatttt gtcaaagtat aatttaatat tagtttctaa gccatattat
    36421 ggagttaaag ttgaaggtaa ggaaatagat attcgaagat gtatttctaa taatatgata
    36481 aataggaatt ttgaaaacta tataattgga attacagata gagaaataga attatttaat
    36541 aatgtagatt taatagagct tcaaagggta gtgttaagtg aaattaataa atttaatatc
    36601 aatttcctag attttaatct aaaaaacttt attatccatt tagctattac tgtaagtaga
    36661 atacttgatg gttactgctt agataatgtt ttagatgttg ttttaacaga ttttcaatcg
    36721 aatacaactg ttgaaaatat atttaattat attgagagta aatatactat aattatttca
    36781 aaggcagaca gagtttatct atataatcat tttataacta aaagttctct tttggataac
    36841 gtttccaata gggtagatac taaaattata gagtatgttg aagaaatttt agaagtaatc
    36901 aacaatcaat atacttttga ccttagaaat gatagtgtat tatttgatga tttagtactg
    36961 cactttaaat caattctcaa ttctaaatct tataatttaa ataaggtgaa tccccttata
    37021 aatacaataa agagtaatta tcctttagca tttgaaataa ctttaaatgc tatagaaaaa
    37081 gtttttaaaa atagtattta ctctttaaca gaagatgaaa ttggatatgt atccctgcat
    37141 ataggtgctg gaatagagag attttttcaa agtaatatta agtgtaaaaa cgtagtatta
    37201 gtatgtggtt cagggtatgg ttcaagtagg ttattagagg ttcaattaaa caaagttttt
    37261 catgataaaa taaatatact tcaatgtcta tcatttaatc aatttttagc tagtgaattg
    37321 tcagatgtag atataataat atcgacaatt cctttaaatc atgatagtat tcctattgta
    37381 ttagtggatt taaaactatt aaaaaaagat atagagaata tatctaagtc aatcactaat
    37441 aattcccata tttatagtaa tcttttggac aatttctttg ataaaaattt atttattgtt
    37501 aatcctaaaa tcaaagataa agatgagctt ataaagttga tgtgcaacaa attaactcaa
    37561 agtgaaattg tatttccatc atttgctgaa tctgtatttt acagagaaag tttatcttct
    37621 accaatatag atgatttttt agctatacct catcctatgg aattaagctc tataagaact
    37681 aagatttgta tttccatatt gaatgaacca atatattgga gtgaaaattc tactgttaaa
    37741 ttaatcatga tgttggctat aaataaagat gattatataa aaataaactc catttatgat
    37801 atccttttaa agataattca tgacaatgat attagagata gtatatctaa ttgtaatgat
    37861 tttgataatt ttctttccat aatcaagtct atagtataga tttttatatt acataagttt
    37921 taaaaacttt aataaaatat tttttgtttt tagcataaat agtataagta agttttgtat
    37981 aatttgttat tattttgtat tgattataat tattatacaa taaagattct gtctcaaaat
    38041 agagagagaa acctgttttt cattgataaa atgataaaaa tatatctgat ttcatacaaa
    38101 aaagagtgtc tcatgaacta aaaagttcat tttgagacac cacctttatt attttatatt
    38161 atttatctct tgtatatatt actatagcat cccttaaaaa cttagctaat ccaggttgtt
    38221 ctctatcata atatgatgta aaacgttcat catatacata catctgtgct agagaggaat
    38281 gagcttcttt agagtatgtg ttccaagtgt aacacaacca tttatgatgt aattttgcaa
    38341 cttcctgagc cagttcacta gaggcatcac cagttttaaa agctttttta agtttactaa
    38401 ttatttcgat atttaggttt tgccaatttt cataatcttg cttagacata tttttaagtt
    38461 ttttatttga tgtatctata atatctttac catatttctc tctaatttca gttccataat
    38521 ttttttcatt ttcatcaatc atcttttctt taaaaccttc aaatttttct atatctgtca
    38581 tagtgtattt tccctccttc agagctatag ttttagttac attttctatt agtaaatcaa
    38641 tttgctttct tttggctagt aatttattat ggtgttcttt taacgcactt aaactatcga
    38701 agtttggtga atttataatg ttttttatat tttctaagtt aattcctaat tctctataaa
    38761 aaagtatttg ttgaagttta ttaacttcat tttgaccata tatacgataa cctgaagaat
    38821 ttattttaag tggcttaaga agacctattt catcataata tctaagtgtt ctagtactta
    38881 taccagctag tttacttaat ttttgaactg tatattccat aagtagtacc tcctataaat
    38941 taaatataca cttttacgca acgtaaatgt caatacatta aaatataaaa atttatatct
    39001 tttaataaaa atataaaaaa atatctttac tggatatttt acacacaact gtcaatactc
    39061 actagtgaaa attaaaaaat aaaatttata gactatcttt actaaaaaat agaaggtcta
    39121 tagtctagga ggaactgtat gaatttaaaa agaagcaatg aaaaacgaga agagtacaga
    39181 agaatgtatt cagataaaga aagtttttta agtttgattc aaaattttac aagtgtctca
    39241 atagcaaaag aattaactct taaaaatata gagttagaaa catcttttat atgtgaatat
    39301 aaaggaaaag aagttgatat aatttataag gtattttcta aaagcggcaa agtatcacat
    39361 tatatagtgt tagaatttca aacagaaatg gatactgaaa ttgtcccaag attgaaatcg
    39421 tatagagaac aaatttggaa aagttttatt atgaaaaaaa gccttgaaga aatagaagat
    39481 aaaaatttta aacttcctaa agttatacca gttgttttat atagtggacc tgaaaggtta
    39541 agtagaaaaa aaggaatttt ggatattgta gaatctatat cggagttcag taataattct
    39601 agtatgaaaa attaaaatta cataatgtgt ttattggtct aaaatggaaa actgtcgcat
    39661 aaacttgaat atgaaaatat ttatagatac aggttctatg tggaggtgtt ccatgagaaa
    39721 taacaagtct aaggtattgc taggaatact ttgtctagca acaggaatgg ctgtaattct
    39781 gtcaatgttg cttcctggct ggatttggtc agcacttacc gcattaatac ttattggatg
    39841 tggagcatta ttattttttt gttaaaatag ttttaactat aaagtgtggg gtgagtattg
    39901 atgaaagtag taacaataaa attaccaaaa tggatttcaa gtataatcct aaaatttatg
    39961 agaaagagta aaaatgatta aattaaaaag cctacttacg gaagtaggct tttaaacgtg
    40021 gttactaagc tctttcaatt ttaccagaac gtaaacatct agtacaaact tttactctct
    40081 taggagctcc atctataatc gctcttacgc ttcttaagtt agctgaccat gttcttttat
    40141 tatgtttatt tgagtgtgat acttggtttc cagaaacctt acctttacca catacgctac
    40201 atacttttgc catctacgca cacctcctta aaatgataac tatttaaaga ctaaaaacat
    40261 atttatatta tcataaataa caaaaatttg caatagaaat atatatttgc cttacacata
    40321 ttgaagaatt tcttattatc atataaaatt atatatataa ttttatataa agtaaatata
    40381 tttggaaaat aattattatt tttctttgaa tatatatact atagaagaaa ttttatccaa
    40441 aaacttttct aggtttaaat tataccatat ttatagtatg atatgattat aattttatat
    40501 aataaataag taaaaatagt aaaaaataaa ttaagaaatg tttttaataa aaataagctt
    40561 aagggggtca accatgagtg caaaggtaac gaatcaatat ggaaatatag aaatagataa
    40621 tcaagtaata gctcaagtcg tatatagagc tgcaatggaa agttatggct tagtaggttt
    40681 agcatataag tctaaaggaa tagtagagct attaaaaggc gagaacgcaa ctaaaggtgt
    40741 aagagttgag gaagtagaag aagatgccat agcaatcgaa ctttatgtta taatacaata
    40801 tggtacaaat atatctactg ttgccaataa tataatagat agagtaaaat atgtagttga
    40861 aaaaatgact ggcgtaagag ttactagaat tgatattaat gtacaaggaa taagagttaa
    40921 gtaatttcat aggaggaaac aaatgattca atatatagat ggtaaaaggt taagggagat
    40981 gtttatttca ggtgcaaaca atctccaaaa caataaagag ttagttgaca agttaaatgt
    41041 attccctgta ccagatggag atacaggaac taatatgtca ttaacaatat cttatgcctt
    41101 aaaagaatta gcaaaagttg aaaatgataa tatatcagat atcggaaaag cattatctaa
    41161 aggttcttta atgggagcta gaggaaactc aggagttata ctttcacaaa taatcagagg
    41221 tatagctaaa tctatagaag gaaaaagtaa attatcaaca gaagatttag caaaagcttt
    41281 taaaaatggt tcagacacag catataaagc tgtaataaaa ccaattgaag gaactatact
    41341 tactgtagtt agagaaagtg gagagtttgc aataaaaact gcgaaaaaag agaaagatgt
    41401 agtaaagttt ttaagtatgc ttgtgaagga atcaaattct tcattagaaa gaactccaga
    41461 ccttctaaag aatctaaaag aagctggtgt ggttgactct ggtggaaagg gtcttgtact
    41521 tatatatgaa ggaatgttag cttctataaa aggcgataac atagaaataa aaaatgttga
    41581 cctagatact aatatttcaa caagtatgga ttttgcaaag agtactacta gcacagatga
    41641 tataaaatat tgctattgta cagaatttat tttagaaagt tctaaggtag aagatactaa
    41701 aataagagac ataatgatgg cttatggtga tagcctagca gtagttggag atgatggagt
    41761 cataaaggta catgtacaca ctaatgaccc aggaaatgta cttcaagaag ctctaaagta
    41821 cggtcaatta ttaactataa aaatagaaaa tatgaagtta caacatgaaa atacattact
    41881 ggatgtagaa gaaaagaaag aaaatgatag tgaaccttta gaagaagaaa aagagtttgg
    41941 gtttatagct acttctatgg gtgaaggttt ggcaaatata tttaaagatt tcggagttga
    42001 ccatataata gaaggtggtc aaactatgaa tccaagtact gaagatttta tgaatgctat
    42061 aaagcatata aatgctaaaa atatatttat tttcccaaat aacagcaata taataatggc
    42121 tgctaatcag gcaaaagaat tgagtgataa aaatataata gttataccta caaaaaatac
    42181 gcctcaagga tttgctgcat tagtaacttt taatggagaa ttaagcgaag atgaaaataa
    42241 agaagctatg atgaatgctt taaactcagt taaatcaggt caagtaactt ttgctgttag
    42301 ggatacagtg atgaatgaaa ttgatgttaa agaaggtaat ataattggaa ttgcagaagg
    42361 aaatttatta tcagcaggag attatgtgga tgaagtaaca tcaaacctta ttgaaaagtt
    42421 ggttgatgaa gatactgcta taataacatt attctttggt gaagatgtta cagaatctca
    42481 agcaaatgaa ctacgtactt ctttagaaga aaagtttgaa gatgtagatg tagagttata
    42541 ttatggagga caacctcttt attattactt aatttcagtt gaataatgat tataagaacc
    42601 tgtatgacca agtagctttt ataaaattgg ttgtacagga tttttgtgta gtaggtgata
    42661 tatttgttgg atttatataa agatgttcaa tatgttaagg gaataggacc taaaaaagca
    42721 gataaattaa ataaactggg tatatttaca ctaaaagacc tattgtatta ttttccaaga
    42781 caatttgaag atagaaataa tctaaagaaa atagcacaat tagaagatgg tgagaaagtt
    42841 acgataaaag cagtaatatc aagtataaat acatttagcc caaaagaagg tatgacttta
    42901 acaaagattg atgtaaaaga tgagacaggc tcggctaaat tagtgttttt taataagagt
    42961 tatataaaaa atacttttcg tccaggagat tcaatattag tttttgggaa ggttaagaaa
    43021 aaatttaata acttagaact tacatcatgt gaattagaat atcttactaa ttcacctaag
    43081 aatacatgta gatttatgcc tgtgtaccag cttacatacg gtgttacaaa taaagaaatt
    43141 atgagtataa ttagaacagt acttgaagat aaagaattaa ttatacaaga atatatgcca
    43201 caaagaatta tagaaaaata taggctatgt agtatagatt ttgcagttag aaacatacat
    43261 tcaccaagta gtaaggaatc attaaaaata gctttatata gaattgtatt tgaagaatta
    43321 cttatactac aactagggtt gtttgtattt aaaagtggca gaaataaaga agatggaata
    43381 aagtttgaaa caagtaaaga cttaaaaaaa ataataagtg ctttaccatt taaactgact
    43441 aaagctcaaa acagggcatt agatgaaatt atacaagata tgaatttaga gaaaataatg
    43501 aatagattgg tacaaggtga tgtagggagt ggtaaaacag tagtagctct cttagcactt
    43561 gcaaattgtg ttttaaatgg ttatcagggc gctttgatgg cacctacaga gatattagca
    43621 gggcaacatt atatttcact tactgagtct ctaaaagatt ttggaataaa tgtaggtcta
    43681 ttaataggta gtcttacaaa aaaacaaaag gatacagtat tggaacaaat aaagaataat
    43741 gagattgata tattaatagg tacacatgca ctaattgaag ataaggttga atttaataat
    43801 attggtttag ttataacaga tgaacaacat agatttggtg ttatgcaaag gagcaaactt
    43861 tcattgaaag gagcaaatcc tgatatatta gtaatgacag caacaccaat accaagaact
    43921 ttagcactta tactctatgg agatttagat atttctatta tagatgaact tcctccagga
    43981 agacagccaa tagaaacaat tgctattgaa aaaagtaagc gagatagagc atataataat
    44041 cttgtaagaa gagaggttga atcaggaaga caagtgtaca ttgtatgtcc cttggtagaa
    44101 gaaagtgaag ccatagaagc caaatcagca gttgaacttg ttgaggagtt aagagcggaa
    44161 tattttcacg acttaaggct tgggttgctt catggtaaaa tgaagtcaag tgaaaaagat
    44221 gaagtaatga gactttttaa agataaagaa atagatattt tagtttcaac aacagtaata
    44281 gaagttggtg tcaatgtgcc aaatgcaaca ttaatgataa tagaaaatgc agaaagattt
    44341 ggacttgcac aattacatca gcttagaggg cgagttggaa gaggtagtca taaatcgtat
    44401 tgtgtactaa tttatgattc taaaacagat gtatgtagac aaagaatggc tataatggaa
    44461 gagaccaatg atggttttaa gatttcagag aaggatttag aaataagagg acctggggaa
    44521 ttttttggga caaggcagca tggtcttcca gaacttaagg tagcaaactt atttaagcat
    44581 ataaaaatac tgaagttagc acagcaagaa gctcgatata tattgggtga agataataat
    44641 ctacaattaa aagaaaacat ggcactcaaa aaagaaatta tagataaatt taaagataca
    44701 ttgaaagaaa tttcattaaa ttaattttaa attgtgttaa aatattacta atgtaataat
    44761 gtgctgaagg gagattgtca tattgagagt aatttcagga aaagcgagag gattaaaact
    44821 aaatactcca aaaaatgaag atgtaaggcc aactacagat agagtaaaag agtctctatt
    44881 taatatgata aattcatata taatggaaag tgaagttctg gatttatttg ctgggactgg
    44941 ttctttagga atagagtgct tatctagggg tgccaagtca tgtacttttg tagatattag
    45001 taaggaaagc atagatattg ttaagtccaa tataaaaaag gcaagagttg aaagtgaaag
    45061 tattatttta aaccttgatt ttaagactgc aatagataaa cttaaattac aaaatagtaa
    45121 atttgacata atttttatgg accctccgta ttataaaaat atgtttatag aagctattga
    45181 aaaaattgat aattctaatc tattgaacga agatggaata atagttgtag aacatgatac
    45241 gaatgattta tttccagata agatatgtaa attggagaaa actagagata agaaatatgg
    45301 aaatactaca ttaacttttt ataagatgga ggcataatat ggaaaataaa cctagaaaag
    45361 ctatatttgc aggaagtttt gacccaatta ctaatggaca tttagatatt atttgcaggg
    45421 cttcgaagtt atttgacgaa ttacaaatag gagtattaaa taatcctaat aaaaagggtc
    45481 tatttagctt tgatgaaaga gtaaaactta tagaaaaaag tacaagccat ttaaataata
    45541 taaaagttgt gacttttgat ggtttattaa taaattactg tcaggaaaat gggattggag
    45601 cattagttag aggtgtgaga agtggtgctg atgttgatta tgaacttcag atggcacata
    45661 tgaatagaga gcttaatcca gatatagaga ctataatttt accaagttgt acaaaatatt
    45721 catttataag ttcatcttta ataaaagagg ttttgctctt tgatgctgat ataaaaaatt
    45781 tggttccaaa gattgttttg gaagaattaa agaaaaaaac tagtgggggt aattgatata
    45841 tgaagataga tttagaaatg attgaactat ttgagcagtt agaggagata tttagaagtg
    45901 cttctacaat ccctttctca cataaatgca cagtggataa agatgaggtt ttagcaataa
    45961 ttaatgatat aagaacttta ataccagaag aagtaacaca ggcagtatgg attaataaag
    46021 aaagaaataa aataataagt caagctaagc aagatgctac aaatatagtt gaacaagcga
    46081 aaaaagaagc tgagagaata caacaagaat atcaagataa tatagaggaa ttaaagaaaa
    46141 attctgaaga tgtagtaaag gcatatgtag aatccagtga acctgttgtt caagcagacc
    46201 aaagagcaaa agatatagtt gatagagctg agagaatagc caatgagata agaattgggt
    46261 caattgaata tgcagaggat gtgctttcta gtgttgagta taatttaaga gaaatattag
    46321 aagaagtaaa aagagataaa gctgaactta gacctaaaaa ataaatttta aataaataaa
    46381 aatggagcta tcacacaaaa aattccaatt tatgtgtgat agctctcttt taatatagca
    46441 attttttgtt atataattgt gctttttaga ttacttatat cttattttga tattataatg
    46501 tagtttaatc ttttatttgt tgaaatcaac ttatttattt gtataaataa tataggttat
    46561 tttcaaattt ttttaataat tttaccactg tcttctttta gattagagtt agactgtgca
    46621 aaattataaa ataatactaa atcatgtgca attatttggt atggcactac atgtagaaaa
    46681 tatagataat aaaattaagg aggtagtatg aaggaaaggg ctaaaaaaat attagatttt
    46741 attattaaaa ataacaatgt aacatcacaa gaactacaag aaaagtttaa tgtttcaaag
    46801 agaactattt attacgatat tctagctata aatgaacaac taggaaaaag tggtaatata
    46861 aaaaatgtaa aacacaaatt tatatttgaa ggtaatttat gtgatgcaag gaaaataatt
    46921 tcaactgaag aagataaatt tttagatagt gattacagaa aaacttttat attaaacaag
    46981 atattacttg gcgaaaaaat atcaatagaa aaactaacaa atgaaatgtt actttcaaaa
    47041 aacacagtag tacaaacaat aactgatgta aaaaaatacc ttcaaacaat ggggttaaga
    47101 ttagagtata agggaaagta caaaattata ggtgatgaat acgttattag agagctgttt
    47161 ctgataattg ttcaagaaaa tatattggaa attaattcta taagtgagga agtaagtagt
    47221 ttcgatacaa aaggacatat aaaacttact gattattctt tattaaatct aactaagttt
    47281 gtggaatttc taaataaaag gataagagat gggaaaacat tatactcata taaatatcta
    47341 aatgaagcta aaaaaattag ttactttagt aattgtaaag aacttttatg tgaagaagct
    47401 aatgaaaatg aacaagccta tatatgcact tacataagtt cacttcccag cttaaatagt
    47461 gaagttaagg aagatgttgt tgaagaatat gtagataaac ttatagataa gtttgaagta
    47521 aatacagcaa taaaattaga aagtaagcat gagtttaaga aaaatatttt aaggcacctc
    47581 cattcttctt acaacagaat acgatttaag tttcctatta gaaatccaat gttagatgag
    47641 actaagtata aacatgagtc actttataaa attataaaat caattataga aaatgaagaa
    47701 gagtttcctg tatttgaggg gataagagaa gaagaaatag gatttatagc agcgtatttt
    47761 ggaggatatt taagagggtc tagggataat ggtctaagaa gaaataaagt cttactagtt
    47821 tgtcctaatg gtcttatggt atcaaagagt ttggaaatac aactatataa atacatacca
    47881 accatagaaa tagtaggtat agtatccatt aagcaactaa aagaagtaaa tgtatactac
    47941 gattacataa ttacaactat agatatacaa aatgtaaaca acgtaattgt tgtaaatcct
    48001 cttttaacat catcagatgt tcaattactt atgaacaaac taataagtgt aaaagaaaac
    48061 gagaagtatt tcaatcttga attaataata caagctataa gaaaaaatgg agttatcaat
    48121 aatgaagaag cactaaaagc tgatttatta aacataatac acaagataga tgaaggagag
    48181 atgtatcaac caatgttaaa ggaattaatt aatgcagaaa gggtcaatat aataaaaagt
    48241 gttagagatt ggaaagaagc aataaaaata gcctcaaagc cattgttaga agataactct
    48301 atagaagagt tatatataga aaatatgata aagtcagttg agaaatatgg accatatatt
    48361 gttttagctg atagatttgc tctaccacat gcaagttcta aagaaggagt aaacaagctt
    48421 gctatgtctt tactaatagt agaagatgaa gttgatttat taggaaaacc agtaaatata
    48481 tttatggtat tagctgctgt agataacact actcacatta gagcattagc atcactatca
    48541 gagatgatgt acgaggaaga aaatgtaaaa ctaataatta atggtgataa aagttcaata
    48601 ctagaactta taaataagca aaattaggag gaatgaaaaa tgaagatatt agcagtatgt
    48661 ggttttggtg taggttcatc aatggtatta aaaatgacaa tacaaaaggc tctaaagcag
    48721 ttaggtatag aatgtgaagt agaaaataca gatataaatt cagctagagg tgctgattgt
    48781 gactgtatat tcacaagtca agaattgtta aatgaattaa aaggaacttg taatgtagat
    48841 gtatatccag ttaaaaagta catggattta gcagaagtca cagaagttgt tcaaactttt
    48901 ttaagtgata aaaatgataa gaaatagtat aagttttaaa aagtaaattt aagttaatca
    48961 gaataaaata aaaacttact aaattcaaaa atagtaagaa agagaggagg gatttagtgg
    49021 tagaaaactt aaacagttga cctatcacta aaaaatataa tgcttgattt tatttcaaat
    49081 aatattttac gtaatccatc tatgttaatt ggattaatag cagcatttgg tcttgtacta
    49141 cagaagaaga aagcatctga tgttataaag gggtcactac ttgcagcttt cggaattata
    49201 attatggaga ctggaacagg tatgttagtt agttcaatag caccaattaa tggagctttc
    49261 caaagtataa gtggtggtgc agtaacaaaa ggtttaagtg atgttacttt tactgcaagt
    49321 tatggtggaa ttataggttt agcaatgttt gcgggtttag ctattcacct aatgatagca
    49381 agatttactc ctattaagac aatcttttta acaggtcaca tgctttattg gttcccattc
    49441 atatttgtag cttcaggagt tgaagctgga ttaaatggtg gagtattaat agcttttgca
    49501 gcagtattat cagcactcta ttggtcaata atgccttggt tattaagaaa atatgtgttt
    49561 gatgttatag gagatgattc ttttacatta ggtcatccat ctggttgttt agcacttata
    49621 tcaggatttg ttgctaaatt agtaggaaat aaagaaaaat ctacagaaga tataaacata
    49681 cctcaaagtt tatcattttt tagagaaata tctataagtg gtggaatggc agtgttttta
    49741 atgtttatag ttgttgggat agtaatacca ggtgcgttta aagatagtaa tgagtttatt
    49801 ggaacgtatg caataaagca aggtctttct tttggagcag gtcttataat aatgcttcat
    49861 ggggtaagaa tgctaataaa tcaaataatg ccagcatttc aaggtatatc tgaaaaagtt
    49921 gtgccaaatg cactacctgc ttttgactgt ccaatattat ttaattataa accaaatgct
    49981 gtattaatgg gattcttagt tgctatgcta acatctacag ctatgatttt aatatgtaac
    50041 tactttaatg tatttaattt gctacttata ccacttgtta taacttcatt ctttgagtgt
    50101 ggagcagcag cagtaatagg agaatcacaa ggtggtatac gtggagctat aataggtaca
    50161 gctatagctg caatatttat ggtagtttta gtaggaatat ctgctgtaat ttactcagga
    50221 actattcaaa attggatact aatatttgga ggtaatgacc tttcattatt tggtataatc
    50281 agtaggttta taggttcact atttggagga atattataga tatgaaattt aaatacatag
    50341 atgagataag aagattaatt gatataatag aaaacgaaga aaaagtcaat atggaaaaag
    50401 cagtacaaat ctttgttgaa gcaatagaaa gtaaaaatgc tatattttca tttggagctt
    50461 cacatgcagg aatattaaca gaagaattat tctatagagc aggtggctgt gttcttataa
    50521 atccagtatt tgcaaaatct ctaatgttag atacagcgcc tataactcac acaagtaaga
    50581 tggaaagatt agaaggatat ggtacagctc tagctgataa aactccaatt aaaaaaggag
    50641 atgtagttat agttcattca gtatctggta gaaatccagt aagtatagag tttgctatga
    50701 aagctaagga aaataaggca atagtaatat gtataactaa tttaagttac tcaaaaagtg
    50761 tatcttcaag acattcttca ggaaaaaaac tttatgaagt atgcgattta gtgatagata
    50821 atcatggtca aaagggagat gcctgtgtag agatagatgg attaaaccaa aaagttagtc
    50881 caacttctac tgtaatagca actactgtga tgaactctat tatagctcaa gctactcagg
    50941 aattagttaa taaagggcta aagaatcctc cgattttcta tagtgcaaat attgatggtg
    51001 gagatgagtt aaataagaag atttttgatg aatataaaag tgtaattcat tatgaatatt
    51061 aaggttaaaa aaatatgtta atgaactttg aagtttatca acaaagaata ttaataatat
    51121 ataataaatg ctatcattta aaagctgtag gtaaaaatct acagcttttt attattgtag
    51181 attaaaaaga tgcgtaatta aattttaaat tggagaaaat aaattggaag gtatcaatta
    51241 taaatattag aaacgttgtc atagaaaaaa actatattaa aaaaaagtac atagaaaaaa
    51301 ctgattagac aataaataca tgctttgata atattttaaa tggagtgaaa atgtgtagaa
    51361 ttataatgaa aaaagaaaat aaaaatgaaa aaagaaaata aaatggaggg aataaaagtg
    51421 ggtatgttaa aaaaattatg ttcactagct atggtagcaa ttatgactac aacacttata
    51481 acagggtgtt caagtggaaa tggaaaagat tcaaaaaagg atggcgaaaa agaaaaacta
    51541 gttctttgga tgccaccagt agaaaataat atgaaggaag tttgggaccc tattttagat
    51601 aaatttgagg aaaaaaataa ctgtgaagta gatttacaga taattccttg ggataattat
    51661 tctgaaaaat ttgcaacagc tataagtgct ggcgaaggtc cagatgtagg atatatgtat
    51721 gcagaaatgt atccacagtt tatagaaagt ggagcagtag aagatttgac taactatgca
    51781 actaaagagg ataaagagca atctatttat ataaaaacat ctgaaatgat gggtggaatg
    51841 tatggtatgc cattccaagc agcaaatcca ggggtattat actataacaa ggatatttta
    51901 gagaaactag gagaaaaacc tcctaaaaca tgggaagatt ttaaaagaat ttgtaaaaaa
    51961 gcaacgcaag atactgatgg tgatggaaag atagaccaat ggggattagc tcagggatgg
    52021 ggagctaaaa cttttggtaa cttaaactgg aactggtatc cttatttatg gcaagctggt
    52081 ggagatatat ttaatgatga tttaaaatcg gttaaattta atgataagag tggtttagaa
    52141 gcagctaagt tcttaaaaga attacaagca tatgtgcctg aagattcttt atctaaagat
    52201 agtaatgaaa tgatagaaag tgtatttgga ccagggaaag cagcatttac aataatgtta
    52261 tcttctgctg caacaagtgt atttgatgaa tcattcccgg atttaaattg gggatttgta
    52321 actggactag aagataagaa agcagcaact tttggagcag tagaccattt atctttaatc
    52381 tcatctgcga aaaataaaga acttgcatac aagttaatac aacatatgtt aagtgttgaa
    52441 tcaatgacag aatttcataa agcaatacca agagcaccaa taacaaaagg tgaaccatat
    52501 caaggtgatg aaagattcaa agaaatggtg gaaaatgaca aaaatgtata tagaccatta
    52561 gtagtaggac ctcatggagt tgaaatatat gaatatttat ggaaagaact tcaaactatg
    52621 atttcaggag ataaaactcc aaaacaagcg ttagatgatg ctgcaaaata ttcaaatgat
    52681 ttattagcac aataaaagtt taaagctgta atatgttgta aagtacttta cagattaaaa
    52741 ttagcattgt atttataaat acaatgctaa tatttatatt ggagaggata aaatggaaaa
    52801 cttagttaga gagtttgaag aaaattcaaa aagaaaaaaa acaaaattaa atattaaacc
    52861 atatatgtat atattgccat tgggaataat tttagtggcg ttttatgtga taccaataat
    52921 aatgtcaata tactttagtt ttacaaagta taatattatt agtccagcta cttttatagg
    52981 tttagaaaat tataaaaaat tatttacaga tgaaacatta aaagtatcta ttataaatac
    53041 tattaaattt actgttgttg tagtaccttg ccaaactata ttatcattga ttttggctgt
    53101 ttggattaca ggaaaaggaa atagtaaaat agcttcattt gctaaaggtg ctatatttat
    53161 accagtatta tcatctatgg ttttgatagg tatggtatgg agggctttat tgaatggaga
    53221 aggttctatt atatatcagg tattaggtgc ctttggaata gaatcatcta aacttttagg
    53281 agatagtaag acagcattac ctacacttat gtttatatct atgtggaaaa atatagggta
    53341 tttcatggta atttatattt cagctataat gaatttacca aaacactgct atgaagtagc
    53401 gaaggttgat ggggctacaa aacttcaaga atttataaaa ataactgtac ctttattgaa
    53461 accaactaca ataatggttg tatttttagg gtcgatatgg tcattacaag tatttgactt
    53521 aatttatact gtgacaggtg gaggtccagg tatatctact atgagtatag ttatgcatgc
    53581 atttaactta aactttaaaa actttaattc tggatatgca atgactgtag caaacgtatt
    53641 atttttgtta atagcagtag tttcgatttt acaaaataag cttgtaaaac gagacaattc
    53701 agatttttag aaaggtaggt ataatatgaa aacagcaaga agattattaa gtgatggttt
    53761 acttttattt atagcgtgtg caagtttggt accttttata tacatgttga taatatcatt
    53821 gaagataaca tataactcat atagtttgga tatatctttt tcaacagtaa cccttcaaaa
    53881 ctatatagat atttttacta aaaaaggatt tgcacaatat ttttttaata cagcaatagt
    53941 atctttttca ggtgtattat taaatttagt atttagtaca ttagcaggat attcttttgc
    54001 gaagatggat tttaaaggta gtgataagtt atttttattt atgataatga ctttaattat
    54061 accttcccaa gttacaatga taccactata tataataatg aaacacttag gatggataaa
    54121 tagttatctt gcacttatta tgccaatacc aactgcattt ggcgtattta taatgagaca
    54181 agctatttta ggagtaccaa aagagttatt agaatcagca aagatagatg gttgttcgga
    54241 ttttagaatt ttaatacaaa tagtgttgcc tttaattaaa ccagcattaa ttacactagc
    54301 aatatttact tttatgggag catggaacga gtttatgtgg cctcttatag ctacaactaa
    54361 agatgcaatg agaacattaa cagttggatt atctactctt aagacattcc agataactaa
    54421 ttatggacag atgatggcag gggctacaat aacattttta cctccattta ttttctattt
    54481 gattttacag agtaaatttg ttgaaggtgt gtctttgtct ggtataaaag gttaaatagt
    54541 ctaatttaat aatatataaa gaaacttata taaatatgga ggaatttaaa ctatggaaat
    54601 ttcatatgta ctgaaagatt gcacattcaa acacaataaa tactcagttg tagaagctga
    54661 aacttggaaa gataaggatt tggatattgt agtagctaaa ggtgaaaaat ttgcatttca
    54721 aattatgtta aaagcaacag aagaatttaa ttgtacaatt gataaaagta gtgctatatc
    54781 atggaaaggc ctgggaaata gagtaaggtt agcattaaat atgcctaata gtttagaaaa
    54841 taattttagt ataaatatat taggatatgt tcaagatgat acaaaggcat ttgtgaatga
    54901 tgctatttta agggataaag atgtgttagt agagcaatat ttgcctcaaa cattttggat
    54961 tgaaggtcaa gtaccagaag attttgaaga aaataacctt gatataggta ttgatatatt
    55021 taaaagcttt ggatatgaag atgaagaaaa agtttgtaca attgatgttc ctgtaaaagt
    55081 aagaaatgtt gtgctaaagc ctttagatga aagcaagttc tttttagatt tatggcaaca
    55141 tccaagctgt ttagctagaa tgtataaagt tgagttatgg tctgatatac attttgaaat
    55201 agtcgataac tatttaaaag aattagcatc tttaggagaa aaagttgcaa cagttatagt
    55261 ttcagattat ccatgggcag ggcaaagttg ttataaagtt tataaaaatc catcaaactt
    55321 atatgaatat aacatggtaa gtgtatcaaa aggattggat ggaaagatta aatgtaactt
    55381 tgagagtatg gataggtata taggcatagc tgataagtat aaaatggcta aggagataga
    55441 tttatttggt ctattaggta attggtgtgc tggtgagttt ggaaatcctg tagaaggtta
    55501 taaggaccct ataagagtaa gatattttga tgaaaaggat aaagtattta aatttataaa
    55561 caatacaaat gatttaaaag aatatatagg attagtatta aatcatttaa tagagtgtgg
    55621 attatgggac agagtaagga ttatagctga tgaaccaaat aatccagaag tagtcaaaga
    55681 gtgtatagaa tttataaatt ctactgtagg tactcatcaa gttaaatata aatcagcaac
    55741 tcacgaccaa aactttttag acagagctaa agatgaaatt gatgatatgt caataaattt
    55801 aaaacttact atacaaaatt ataaagatat agaaagtctt aagaaaaaaa tcaatgacaa
    55861 gggtgggata ttgacttggt ttgtatgctg ttttccagaa aaaccaaata gttttttaag
    55921 ttctcctttt gtagaaaata gaataatagg atggtatact tattactttg gtcttgatgg
    55981 atttttaaga tgggactata atttatggac agaagaccct tggaaagatt caagttacaa
    56041 attccctatt tggaaagctg gagatatgtt ctttgtttat cctggaaaag atttaaagcc
    56101 agttagaagt gtcagaatgg agaatttaag atttggaata caggattttg agttatttac
    56161 aatgttagaa aaagaaaagg taagagagta tatagaagtt gagcttatgc aagaattatt
    56221 aaataaaaaa gaaaatgctg aaattaaagg ttttggtgac atagaactag gatattcact
    56281 tgataattgg agatatgata aagttaaaaa atatgtatta gagttgttag acagagtata
    56341 aaaaatataa gagtgtctca aaatgaactt tttagttcat gaggcactct ttttgtatga
    56401 aatcaatata gtttcattgt ttcaacaatg aaaaatagga ttatttctat tctaagacag
    56461 ccctttagtt ttttagttgt attcaatgtt ttaaaactaa ctataaacgc tataaagttt
    56521 ataaatgaca ataattatat ataacatgat gtactttaat ttgctataat agggattgaa
    56581 taatattata tggaagataa cattgtttgg ggggattaat ataaaaagaa taggtattat
    56641 tataatgttt tttttaagta tatcttttat tggattttat cttgtaataa gagataacag
    56701 tgatacagtt aagatgcaac gagggataaa tataggtaat gcactagaat cacctaagga
    56761 ttttccgtgg gatgtaaaaa tgtcaaataa gttttttgat gatataaaag atgcaggttt
    56821 tgacacagta cgtataccag taaggttttc tgattatacg tcagattctg ataattttaa
    56881 aattgatgaa gaatttttta aaaagattga taaatatgta gattatgcat tggataaaga
    56941 cttaatagtt gtgttggatt tacaccattt tgaagaaatt atgaaagaac caagagttca
    57001 taaagaaaag tttttaaaaa tatggcaaca gatagctaat aggtatcaaa aatatgataa
    57061 aaaattggta tttgaattat taaatgaacc taaagaaaat ttatcttcac aattactaaa
    57121 cgaatacatt gaagaagcaa taaagataat tagaaaaact aaccccaaaa gaactattat
    57181 agtaggacca tataatttct atcaaattga ttatttgaat gaattaaata ttcctaaaga
    57241 ttctaatata gtagtttctt ttcactatta cgagccaaat gattttgcat ttcaaggaaa
    57301 tatctatcat aagggatttg agcacttaag caatattact tgggaaggaa caaatgaaca
    57361 aatggattat ttaaaaaaaa ggtttgatac tgtggaaaat tgggcaaata aaaataaggt
    57421 aaaaatcttc ttgggtgaat ttggaataac aaaagaggca ccagaagttt caagaagagc
    57481 atggataaaa gctgtaagag aagaagctga aaaacgaaac ttttcatggg cttattggga
    57541 gcttgcatct ggatttggta tatacaatca aattgaaggg acttgggata gagatatatt
    57601 aagtgcatta atagaaaaga ggtgatttta tgaagaaaaa ttcaaaaaag atacttataa
    57661 ctttaactat tattactaat gtcatatata tattgtggag aattttttac acagtaccta
    57721 aagaagaagg gaagtttgcc ttaatatgtg ccataatact tctttttgta gagataatgg
    57781 gtatgatgga aatgtttgtg cattattatg gtatgtcaaa tattgagtat cctgaaaaac
    57841 caatcataag tgaagaactt tatccacatg tagatgtatt tattgcaaca tataatgaat
    57901 ctgttgattt ggttagaaaa actgtaaatg gatgtattca tatgcagtat ccagataaga
    57961 aaaaggtaca tatatacata tgtgatgatg gaaatcgtga agaaatgcgt attctagcag
    58021 aaaaaatggg tgtaaattac ataactagaa ctgaaagaga aggagcaaaa gctggaaatt
    58081 taaataatgc aatgcaacat acaacttctc ctttgattgc tacatttgat gcagatatga
    58141 taccaatgca tgacttttta attgctacag taccatactt tttaaaaaat gagcaagcta
    58201 aaaaagatgg agaaaaagag gagtatgaaa aggttggttt tgtgcaaact ccacagagtt
    58261 tttataatcc agatttgttc caatttaatc tgcactctga aggaaggatt ccaaatgagc
    58321 aagattattt ttacagagat attcaattgg ctagaaatag gacaaattct gtaatttatg
    58381 gtggtagtaa tacagttatt tcaagggaag cacttgaaga agttgatgga ttttatactt
    58441 attctattac agaagatttt gcaacaggta tcttaattca aagtaagggg tatagatgtt
    58501 atgctattcc agaagtacat gcatcaggtt tatctcctac agatttaaaa agtcttatta
    58561 agcaacgtga aagatgggca cgtggatgta tacaaactgg tagacgtcta aatattttgt
    58621 ttaggagagg tttaggtttt tggcagaaaa taagttatat ttcttcaatt acttattggt
    58681 atgcaagtat aaaacgcttt gtatatataa tggcacctat tttattttct gtgtttaatg
    58741 tgattgtagt aaaatgtacg ttacttcaag tattggtatt ttggttgcca atgtatattt
    58801 taagtagttt atctcttaaa atattttctc aaaatataag aaatactaga tggaccaata
    58861 tatatgaaac tataatgttt cagtctttaa tgccagcagt aatacttgaa acgtttgcaa
    58921 tatctaaaaa taaattttca gtaacaaata agagtaaatt agaagaaaat agaatgtata
    58981 agtttttaca gggaatacct tattttattt acatggtctt atctatcata ggaattttaa
    59041 aaatgtttgt agcaatattt aaaatgagtt caatgacata ttcagttgta ttattttggt
    59101 tgattggaaa cctttttaac cttgttatgg ctacattatt tatatcaggg agacagcagt
    59161 taagaaagtc tgagcgttat attgctgaaa ttgattttaa gttaaagcaa aattcttatg
    59221 ttctttcttc aaaaacaatt gatatatcag aaaatgggtt tgcatttttg ctagaaaatc
    59281 cagaatatat ttcaccagaa gaagagtttg aggtagagtt tagagaaaaa tctggaaatg
    59341 aaatgtatat agccaatatg aaagcaaaga ttgttaatgt tgtggaagta aattcaaagt
    59401 ggaaatatgc agcttatatt acacatattg aagatagtga aatagataat tggatgtgta
    59461 ttgttcacga tagaatacct actttaccaa tgacaatatc taatcaacta ggtttctttg
    59521 atgatttgca aattaatgtg aaaaaacgta ttgaaaaaac tagaacatta tcacgaagaa
    59581 gtccaagaat caatatgaat tttcagatgg atataaaaaa tacaggtaaa ttaagaatag
    59641 tgaattttaa ttatcaatat gtattattaa attttgagaa taaaagtatt tatcctaaag
    59701 aaatagcatt ggaaattaat gaaaatattg tgttagaatg tgatttgtgt gagggtaaaa
    59761 ttgatgaaag aggtatctta tataaagtaa ataacattga cagcataatg caaaatttct
    59821 ttttaagaga tgaaatgatg gattggatat tacaaaataa gacaattctt gtttcaaagc
    59881 ctagtgaaaa gaaggaaaaa agtatagatg agtttgaacc catggaatat atttaggaga
    59941 gatgaaatga agactttaaa agtgtttttt tgttctacaa aatatcttat tttggtgttt
    60001 atttttttta taaatactac ttttataagt tcagcaacaa gtgataaaat tttagatttg
    60061 tcctttaaaa agatagagac tgacttaaat tctaagatta catatgaaga tacaggtgta
    60121 aaaatagaaa cggatagttc taaaagcgat aaggaacgtt atttatatat ttaccaaaat
    60181 ataaaagaaa attggagcat gtataataat ttttatattg aaattcaaaa taaaaataaa
    60241 agttctcaga gaataaattt aagcatacaa agtaaaaata tgcttgaatt tagattaaaa
    60301 gaaggtagtg aagtattttt agaaggtaaa aatataatat attcagataa aataaaagaa
    60361 ggatgcatag aagtaccagg tgaatttgaa ggcaaaattt atgtgaactt taatagcctt
    60421 ataaatgagg aaagtaatgt tgtgctggac tcaaatatgc tttcaaacat tgttagttgg
    60481 ggaattacgt ttattccatc tgatgaagaa cataatattg ttattataaa aaagatatct
    60541 ttattgtctg aagagaaatt aagattttta aataatatta aaataattgg cgatgaagaa
    60601 gtacaaatac cagtattggg tcaaagtata agtcagtatg aagtattagg tcttaaaagt
    60661 gattctaaaa ttaagtactc tcttatggga aaacaagata atgtaagcat atctcaaaaa
    60721 ggtaaattaa cattaagtaa taaagccaaa cctggacaaa ttattttaca agttaatgtt
    60781 gatgataagt ttaaaattgg aaagaaaata acattaactg agtcttggag tgttaataaa
    60841 aaagataagg atggtgttcc atatacatta gtatatcctg agcaatcacc aacagtacaa
    60901 gatatgaaaa aaataaactt tatgaataac atcataacgt ttgttcgaat tttatttgta
    60961 tctctagtaa taatatgctt tggaatatat ttatattgga aaaaatgttc aaaaactaag
    61021 taaagagagg aggttagatt gtgttatttt caagtttaat ttttttattt tattttttac
    61081 ctataacttt ggttttatat tatgtattta ggtttaaccg aacgatacag aacatgatat
    61141 tgcttgctgt tagtttattt ttttatgctt ggggagagcc aaaatttgtt gttatcatga
    61201 tagtttccat aataatgaac tatatatttg gtttactggt agatagatat agggaaagta
    61261 aaataaaagt aaaagttttt ttagttttga tgtgtgcata caatataggt gttttattta
    61321 tatttaaata tctagctttt gcgctaagaa atattagtga acttattagt actgaattga
    61381 ctatacctag tattgtttta cccataggaa tttcattttt tacatttcag ggaatgtctt
    61441 atgttataga tgtatatcgt agacatggag aagtccaaaa gaaccctttt tatgtgggat
    61501 tgtatatagc atttttccca caattaatag caggtcctat agttagatat gagagtgtgg
    61561 cagaacaaat tctcaataga aaagaaactt ggaataaatt ttctattggg acttgtaggt
    61621 ttataacagg tctaggtaaa aaagtattaa tttcaaataa catggctata gtagcagact
    61681 atatttacac tatgaatagt caaggagaaa tagctgcatc acttgcatgg cttggttcaa
    61741 tagcctatac tctgcaaata ttctttgatt tttctgctta ttcagatatg gctataggct
    61801 taggtcttat gtttggtttt aaatttgaag agaactttaa ttatccatat atatcaaaat
    61861 caattagtga gttttggaga agatggcaca tttctttggg aatgtggttt aagagctata
    61921 tatactttcc gcttggaggt tctagagtag caaataaaga tattatgatt agaaatatgt
    61981 ttatagtgtg gttatttact ggaatatggc atggagcaga atggacattt gtaatttggg
    62041 ggattttaaa ctttgttttc ctaattatag agcgatttat aatgtttgaa aagattgaaa
    62101 atcacaattt tataaaacat atttatgctt tattagtagt taattttggg tgggtattat
    62161 ttaggtcacc taatttaaaa gaagcatata attattttaa agctatgttt ggaggcaatg
    62221 gagctatatg gagtgattat acatatatgt ttttaaaaga atactgggtt ttctttatat
    62281 ttgcatttat ttttagtgta cctattgcaa agaaaatcaa taagtttgtt gttgaacaag
    62341 ctaaatatag tatgatattt aacgtatttt atccaatctc agctatttta ttgtttttta
    62401 tttctgtgac atatttagta actggaagct ataatccatt tatttatttt aatttttagt
    62461 tgggaggaaa agtatgaata gattttttaa agtaagatta ataactgcaa ttatgttctt
    62521 aattatttta ttcgctttct ctatctttaa tgtttgttat tctggaaaag atacgataca
    62581 ggaaataaaa aattcaatag ctgaaaaaag tagtttaaaa gatacaatat catctattga
    62641 taatacagtg aatgacaaag taatatacaa aaatacgttc attgaaactt atggctattt
    62701 acaaaaatta atgggtaaaa atgagttttc aaaatttagt gttgtaaaag atacttctgg
    62761 aaagatgcat tatacatatt ttgctaaagc tccaaatcca gttggtgtga tagcagatag
    62821 ggtaagaaaa ttttctgatg aaatggaaaa gcaaaacact aaattggttt acctaatgac
    62881 tccagataag tatataaaag gagtgacaaa atttccaaaa ggaatacctt attcttatag
    62941 taatgagaca gcagataatt ttttaaatca actaaaaaag tataatgtgg actatattga
    63001 ttttagagaa aatattttaa agagtggtat tccaaaagat gatttatttt ttaaaacaga
    63061 ccatcactgg aaagtagaaa cttctttttg ggcatttgga gaattagttg agcaacttaa
    63121 taaaaaatac aatatgaatt tagatgaaaa tcattattat agagataagg acaattataa
    63181 ttcaattgta tatcctaagt catttcttgg ttctatggga agaaagactg gtatcttata
    63241 tggtggtata gatgatttta cattaattta tcctaaattt aagactaatt atacttatta
    63301 tacagattct aaatctcaaa aatttgaatt aaaaggaagg tttgaagaga gtttaatatt
    63361 atcatatcct tttaatgcag accttgattt aatggatggt caatcagata agtattttac
    63421 atatttattg gggaacagac cattagttaa aataaaaaat atagaaaatc cagatggatt
    63481 aaaagtgctg tttgttaaag attctctaat tgtaccaaca gcagcatttt tttcctctgt
    63541 ttgttcatct atagatatga tagaccctag atattatgat ggagatatac tagaatatgc
    63601 aaaatcccat gactatgact ttgtttttat atctgtatat ccccaaaatc ttacaaaaga
    63661 attttttccg ttttgtgagt aaatttctaa aaaagttttc tgataaaata aatggctaac
    63721 tggtaaaaag ttagccattt attttgagtg ttattctaag aaatattttt tttactaaat
    63781 acttctttat ctatttttcc aagtgatttt cctacagtaa ttgatgatac catagcacca
    63841 ttaacattta tcatagtacg tcccatatct agtatagggt ctattgcaag tataccacca
    63901 actaatggaa aataagaacc aagaccaact ccagagataa caactgaaac ggccattgta
    63961 gcagttcctg gaagaccagc tattccaaac gaactaattg ttataacaat taacaacata
    64021 acataaaagc tcatatccat gtttactcca gccatattgg caagtgttac agccattaaa
    64081 gcaggatata tacctgcaca accattcata cccatatttg cacctaaact tgctgtaaag
    64141 cttgatacac cctcatcaac tccgtgattt tgtactagac tttctatcgt tacaggcaat
    64201 gttcctaaac tagaacgtga agtaaatgcc agaagtaaag cttcagttga attttttata
    64261 taagtaatgg gatttagacc atttaaagtt ataaaaatta aatgtataat aaataatatt
    64321 gcaactgata tataaagagc taatataaac tgtaaaacag atactattga actaatacct
    64381 tgtgaagtta ttgtatttgc aagcagagca accacagcat aaggcataaa tttaataaca
    64441 gtcatagaca cacttacaat tattttgtaa gatgcttcta taaaacctat aaatggtttt
    64501 attgtttcaa aatgtttttt acttaatctc tttattgagg tagctataaa tgtagcaaat
    64561 attattgtag ctacaatatt tccatttgcc atagccatta caggattaga tggtagtaat
    64621 cctcttaaag tatccacaag agagtttatt tctttaagtt cttgattttc aactaaagta
    64681 ggcatattag ctcctacacc taacttcatt atatttgcaa cgataattcc aacaattgca
    64741 gctatagcag tagtagcaag aagcatacct aaagatttaa atgtcagagt tccaagattg
    64801 ttatctttca tatttataat tacttttata atggataaga aaactaaagg tactactaac
    64861 atttttaata agtccataaa tccagaacca aataggctat accacttact tacttcttgt
    64921 agccaaatga cttgagttgt atcattagga aaaccagcaa aagcttgtat tatgattccc
    64981 aatatgagtc ctagtactgt agcatatatt gttctcaatg aaaattttat ttttttcttt
    65041 tcaaattgtc taacaataaa gaacgtacct agaagtaata ttatgaaaaa aattgtttta
    65101 aaatctgtaa tcataagaaa ttgtgataaa aatgaacttt ccaatatttt tctcctttta
    65161 tttaaaaata aatttttcta agtttaaatg atatcaatat attaaaaaaa tgtcaatatt
    65221 atatcacaat ttacttaaat agagtaaaac aagttgattt tgaaaaatgt gattaagtac
    65281 agaaaaatag ggtataataa atgtttaaag aatttgaatt gtggtaagaa aataaaaaat
    65341 agcttttata agatttgatt tcaaattaaa atgtgttaaa taaataacta aaaattatta
    65401 taaaaaatgt tttcatagaa tatatatcaa atgaaaagct taaaataagt gaagaaatta
    65461 actagacatc aaaggagaga taaaatgaga gtaataataa ttggtggtgt tgcagctggt
    65521 atgagtgctg cagcaaaatt aaaaagaata aaacctgagt atgaagttgt tgtttatgaa
    65581 aaaactgaaa tagtatcttt tggtgcttgt ggattaccat actttgttgg tggatttttt
    65641 aatgatgctg atgaattatt agcaagaaca ccagagaaac ttagagaagc tggaattgat
    65701 ttaaatatat ttagagaagt cgttgaggtt gatagtgaat ctaagaaaat aaaagtaaaa
    65761 aacataaaaa cagatgaaat atatgaagat tactatgata aattgatgat agcaactgga
    65821 gcaagaagta ttatgcctcc tataaaaaac ataaaattaa agaatgtttc tacattaaaa
    65881 agtttatatg atggagaata tttaaaaaaa cttttaagta atgaagataa taaaagagtg
    65941 acaataatag gagctggttt tatagggtta gaagcagttg aggcatgtaa gaaattaggt
    66001 aaagatgtag atgtaattca gttagaagat agaatattgc cacaagtatt tgataaggaa
    66061 atgacggatg tattagaaga agaaataaat agacataatg ttaatttgca cttagatgaa
    66121 atggttgtag aactttgtgg agaagataaa gtagaaaaag ttataacaaa caaaggggaa
    66181 atagatacag atgtagtaat aattgctact ggcgtgagac ctaataccga gtttttgtca
    66241 aatactaata taaaaatgct aaaaaatgga gctatagtag ttgatgagta tggaagaact
    66301 tctgtagaag atatatactc tgctggagat tgtgcaacta taaagaatat agtaagtaat
    66361 gaaaatgtat atgttccttt agctactgga gctaataaat tgggtaggat agtaggagaa
    66421 aacctagcag gaagagaaat atcttatcaa ggttcactat cttcaagctg tataaaaatt
    66481 atggatatgg aggcagcatc aactggaatt acagaaaggc aagctaaaga tttaggcata
    66541 aatgtcaaat ctaaatttat ttctgattat aatcaaactc attattatcc aggaagaaat
    66601 aaaatatatg taaagcttat ttatgatgca gatacaaaag ttattttagg cggacaagtg
    66661 gctgggttta aggatgctgt ccaaagagca aatgtattgg cagctgctat ctttggaaaa
    66721 atgaccacaa atcaacttgg aatgttagat ttatgttatg ctccaccatt tgcaagaaca
    66781 tgggatgttt taaatgtagc tggaaatgta tctaaataat atttagatta tagctaatat
    66841 agttgacaag caattaaggg aatgaattta cctaaactat ttttatcatg gtaaaattca
    66901 ttcctaaatt tttattccaa atttttattc caaattttta gaaataagga ttccattaaa
    66961 ttattcatca aaatttaaac gcaagcttaa tctatcttct ccaagaatag tattttcaaa
    67021 aacattagta gcattttcta aatacatact aggtatgtct aaagaaggac taaaatgagt
    67081 cagtaaaagt tgtttgacat ttccaagttt tgcaagatta gcagcttctc taaaagtcat
    67141 atgtttgttt ctaacagctt ttgatatatc taaatcatca ccatacatgg cttcacatat
    67201 aaataaatca ctatctttta tgaaatctgg tattgattct ataggtcttg tatcggttgt
    67261 aagacttatc ttaactccct ttctcaattc tcctaagacc atatctggag tgtattgttt
    67321 tgaatctaaa actatatttt gaccttcttg gagtttgttc cataatattt taggaacttc
    67381 gtttttagtt gccttatcta tatcaaattt agggtttctt ctaaaattaa aactgtatcc
    67441 taaacactct atagagtgtt ctaaactgat tgttgaaatt tctaaatttc ttaaaacttt
    67501 attatctaaa gaaaaattgc cttgagggtt ttctataatt tttaaagtgt atggtacata
    67561 ttctactaaa tttctcatag aacgtataca atcgactatt ccaactggac caactatagt
    67621 taaatcactt gtacgaccac tattcccaat tgttgataaa agtcctaaaa gtccaaatat
    67681 atggtcacca tgtaaatggg ttatacaaat caagtctata tctttaaagc cacatttttt
    67741 tagcttcata gatacctgag taccttcacc acagtcaatt agtatttttc tacctttata
    67801 gtttataaat acagaggata gaaatctgtt tggcataggt acatttccac cacatccaag
    67861 taaagttaaa tctatcattt tttccccttt ctaaaattaa cacaaatctg ttgtttgttt
    67921 ttattatgat atcctattat aattatttta acattaaata taacaataaa tgtgtatttg
    67981 agtcaagaaa tattgtcaaa taaagttttt atatagcaat gtataaaaat ttggaaaacg
    68041 aaaagtgaaa attatattta ggagtattta aaggaggaaa atttaatgaa agtattaaag
    68101 aaaattacta ttttgtcatt aattacatta accttagttg gttgtagtaa cggagaagag
    68161 aaaaaagtgg ttgaaggttc taagaaaact caaaattcta aaaaagatga acagcctata
    68221 atagtacata aagatgcaaa tatattgaaa gataggtatg agccaccaaa aggatttaaa
    68281 agagttgatg tagaaaaagg tagttttgaa tcatttctta ggaatactaa gctaaaaaat
    68341 tatggagaga aagtaaagta ttatgatgga agaactaagg aatcaaaagg tatttatgat
    68401 agtgtatttg atgttgatat tggaaataga gatttacatc aatgtgcaga tgcaattatg
    68461 cttatgagag cagaatactt gtatcagaat aaactatatg atgaaataag ttttgatttt
    68521 gttgatggtt ttaaggcgaa gtattcaaaa tgggcacaag gatatagaat ttcagtaaaa
    68581 gatagtggtt caagctggta taaagcaact gaatattcaa catcttatga gagtttcaga
    68641 aagtttatgg atatagtgtt tgcatattca ggaacgttat ctcttgaaaa agaattggag
    68701 cctgtaaaag ttgaggatat gaaaatagga gatgtgttta tagttggtgg aagtccagga
    68761 catgcaagta taattatgga tatggcagaa aatgaaaaga ctggtgaaaa ggtatttatg
    68821 atagcccaaa gctatatgcc tgcacaacaa acgcaacttc ttattaatcg taaaaatcca
    68881 aatttgagtc cttggtatag tttagatttt gaaggtgatt tgacaatacc tcaatggact
    68941 tttaaaagag accagttgaa aagattttaa aataataatt ttatataata aaagaaaaat
    69001 atagatgtga aaaaatgtaa ttagattaaa actaattgga tatggacaaa attaaattga
    69061 tagataaata ttaaaaatta cactaattaa tcttactata gtttaatatt tatctaaaaa
    69121 gttttatgta aaatataaat tttatattaa agctaaaact aatttgagga ggctaattta
    69181 tattgaagat tagaaggtat ttaattttgg taattataat tctaattaca acaagtcaga
    69241 gttttatttc ttatgggcta gagcgatttg aagaaataac catttttcac actaatgata
    69301 tacatggaag atatgctgaa ggtgatgacc atatacaaat aggaaatcta gccacattga
    69361 aaaaagaaac tccaaactca attttagtag atgcaggaga ttgtttgcat ggacttccaa
    69421 tagtaaatat ggatagaggg aaaagtgcaa ttgaattaat taaatgtgct ggatatgact
    69481 atattactcc tggaaatcat gactttaatt atggaaaaga tagactgttt gagttatcta
    69541 aaagtgctaa tttaggagag aatactttaa aatttttatc atcaaatatt tttgaaaatg
    69601 ggaaaaaagt ttttgatagt aatgatataa aagaaataga tggaataaaa ataggatttt
    69661 ttggactctc aacccaggaa acaaagagca aaactggatt taaaaatgtg gaaggattag
    69721 agtttaaaaa tcctatagaa agtgcaacag aacaagtaaa tgaattaaaa ggaaagggtg
    69781 ccaatgtaat agttgcaata tgtcatattg gtacaaatag tttaagtaag ccaaatagta
    69841 tagatatagc aaatgaggtt caaggaattg atttgattat tgatggacac agccacacta
    69901 agtttgaaaa tggtaaacaa gtgggtgata cattgattgt aagtgcagga caatatttag
    69961 aaaatattgg cgagataaaa ctaactttgg atactaatga tgtagaagat ataaaaatag
    70021 aaaataaatc agctagatta attggaaaag aagaagcatt aaagtataag cttgactccg
    70081 ttgtaagtaa aaaggttgat aaaataaaag aacaacaaga aaaaatatta gataaagtta
    70141 taggggtaac tacaaatact ctagatggtt catatgaaaa tgtaaggaca aaagaaacta
    70201 atttgggtaa tttaataagt gacattttac tagataaaac taaggctgat atttctttat
    70261 ttaatggagg gaatattcga gatacgatag aaaaaggcaa tataacaaga agagatattg
    70321 tagatgtatt tccttttagc aatactatag ttaccaaaga acttacagga gcacagatta
    70381 aagatgtatt ggagcatgga gtaaaacttt atccagaaaa aagttcagcc tttttgcaag
    70441 tgggaggaat ttcatattac tttaatccta aacaaaaaga aggcgaaaaa ataacaaata
    70501 ttcaaaaaga aggaaagcca ttagatttaa ataaaaagta tgtagttgca acgaatgatt
    70561 atatagcatc aggtggagat gaatttcctt gtttttcaaa agagccagta ctaaaggaat
    70621 ttggaaattt agagagtgca gtcattgaat atatagagta caaaaaagaa atatcaaaaa
    70681 atgtagatgg aagaattggt attaaatcta aagatgagtt aataagcaag aatcatgaga
    70741 gtgatttgat attaagtgaa aatgaaaaaa atatcaatga taatgaagag aatctatcta
    70801 ataatataaa aagctctgat agtgataaaa ataaaagctt agataaaaca agttctagag
    70861 aaagttctaa tctaggaata tctaaaagag aagctaaaga aaaatctcct aaaacaggag
    70921 atttaggttt ttcaaatagt ataattatat ttatagtttc aagtacattg atatgtttat
    70981 tgaattttaa tcaaaaagag ttaaaggata aaaagtcaaa ataaaaatta atgagcaaaa
    71041 attagtagat agtaatttaa ttttagataa tagtaaacag caaaggaaat tagttatata
    71101 aatactaatt tcctttgttt tatgttttaa catgattact tttaaataaa attgtatttt
    71161 atgaaaaata attatactaa atggcatagt attatgggga tttttagttt ttattcaagt
    71221 aaatgaaatc atattaacaa cttctgatat atttacaaat tgataaaaaa atgattttat
    71281 tagtatgata taatttagct agtatgtatg ataatggagg atatagggat gaataatata
    71341 ttacttttgg aagatgacaa atctttaaat agaggaatta gttttaaatt aaaaaaagaa
    71401 ggatacaatg tttttagtgc atttggtata gaagaagcaa aaaatatttt cgcaaaagaa
    71461 gagatatgct taattatttc cgatataggt ctacctgatg gtagtggttt tgacttttgt
    71521 gaggagatta gaaagaaaag tgatgtttac ataataatgt tgacagctct tgatgaagag
    71581 gtggatattg tcacaggata tgatttaggt gcagatgatt atatcacaaa gccttttagc
    71641 ttgatggtat taatatcaaa agtgaatgcg cttatgaaaa gagtaaatac agtaaaaaat
    71701 tatactctat tagtttgtga tgatttattc ttttattata tagaaaataa attaattata
    71761 agaacagatg ataaggaaga agagataatt ttaagtaaga cagagacaaa actattaaaa
    71821 tatttgatgg aaaattctat gcagacattg actaaagagc aactgttaga atctttatgg
    71881 gatagtagtg gtaattttgt agatgataat acgattgcag taaatataag aagacttaga
    71941 caaaaggttg aaaaaaatcc atctgaacct aaatatataa aaacagtaag aggggttgga
    72001 tatatgtggg gagaaaggag tataaaaaaa tgctaaaaaa tatgaacctc aaagatggtt
    72061 taagtgtgaa agatgattat ataatgagaa gagttattgt atattctgtt ttatctgtac
    72121 taatagtaac ccttgtattt atactttttg aatatagcag attacataaa ttgtatgaac
    72181 atcaaataaa aaatactcaa aatgttgctg gaatattgat tgaaaaatat cctaatgatg
    72241 aaatagatat agtaaattct atttataatt ctgattatag taaggtgtct actggagaaa
    72301 aggcatttaa gaaatttggc tatggattgg aaaataaaat gtcaaatgat aaaaatttta
    72361 gcttatactt aaaatctttt tttaaggaat cattttttat ttttttaatg atgtttatag
    72421 tagttatagg tataatatat tattttataa gatacataaa taaaagattg agtaaaattt
    72481 attttatagt agataagatg tcacaagaaa actacttgaa ggacactact gagtcagaag
    72541 aaactggtta tctagtagaa tataatgaat actttaaaga aggcatattt agtaagataa
    72601 ataactgtct atatgagctt aatagaagtt taaagataaa gtttattaaa ttagaaaaag
    72661 aaaaagaaag tgtaaaatca ttagtaacag atatttccca tcaattaaaa actcctttag
    72721 catccttaaa attatacaat acattactta tagaagaaga attagaagaa gatgaagcca
    72781 acgagttctt attaaccaat aaggcttcta taaataaatt agaaaattta ataaattctt
    72841 tagtgaacat atcaaggtta gaaattagca tgataaacat aaaaaaagaa gataatgata
    72901 taaaaaatac tattctaaat gcagtaaaaa gtgttacccc taaggctaag agcaaaagta
    72961 taacattaaa tttaaatgaa tttaacagta gaattatacc acatgataaa aagtggactg
    73021 aggagagtat ttttaatgta ttagacaatg ctgtaaagta tacacaggta aatggagaaa
    73081 taaatatatc tgtagaagaa actgctaatt tctttaaaat tataatagag gataatggca
    73141 tagggattga aaaaagtgag tttaataata tatttaaacg tttttataga ggttctgctg
    73201 atgaaattga atctgtagaa ggtagtgggg taggattgta tttaagtaga aagattttag
    73261 aagaacaagg tggaaatatt atagtttctt caaaagtagg aatagggagc aagttttctt
    73321 tgtttcttac aactatgtaa tattaaatga aaggaaaatg taagcctcta catttattat
    73381 ataagtataa acagctccag aaataaaaaa ttaataaaat atggagtaat ataacttaga
    73441 atgagaggat aattttatga aaatactata tactgaaaat ttatcaaagc attatggaaa
    73501 aggagaaagc ttagtaagag ctttggacaa tgttgactta gaaataaatg aaggtgaatt
    73561 tgttgcaata ataggaaaat caggttctgg aaaaagtaca cttttgcata tgattggagg
    73621 tcttgatata cctacatctg ggaaggtgta tatagataat aaaaatatat ttacattaaa
    73681 agaagaagaa cttgctgtat ttagaagaag aaaaataggg tttatatttc aaagctataa
    73741 tttaattcca tctcttaatg tatgggaaaa tgtagtgttg ccaatcggtt tagatggtag
    73801 agaggtagat gaaccgttta taaaagagct tttaaaatca ttaggacttg aaaataaaca
    73861 cgacgtttta ccaaatacat tatcaggtgg tcaacaacaa agggtggcaa tagcaagagc
    73921 attagctaca agaccagcta taatacttgc tgatgaacca actggaaacc tagattctaa
    73981 aacatctgat gaggttatga gtatattaaa atctatgtct aagaagtaca gtcaaactct
    74041 agttatgata actcatgatg acagtatagc ccaaatggct gatagagtta tattcataga
    74101 agatggcagg gtgtcaaagg ttggtgataa aaatgattaa tactactaaa atagcaaaaa
    74161 gtaatttaaa gcaaaataaa tcaaaaagta ttctaataat aattactata atactttcaa
    74221 ccttaatgct ttcttctatt ggaatttata ttgttgatgc aggagcatat caaaaagaaa
    74281 atactataaa gtattcaggt aattatcaag gtatattagc aaatgtagac gaaaaacaag
    74341 ctgatatatt gagtaatcat gcagatgttg aattaactgg taaaatgaat ggagtaggtg
    74401 ttgaaaaact ggaagatgat tcaaatattt ctctagcata catgaatgaa gatgctttga
    74461 aattaaatag ttttgaattt atcaaaggaa aattgccatc aaaagagaat gaaattgtgt
    74521 tagatagtgg agctttaaag gcacttggtt ataaaaataa agatttaggt gaaaagataa
    74581 aaatatctta taatgactat aaaagtgata aaaaaattga aaaagagttc attataagtg
    74641 gaatcttaaa aactagcgaa atatctgaag caggtaaata ttattatgct attatatcag
    74701 aatcatatat gagaaatact agagatatgt cacaagaaga ttttaatata tatgtgaaat
    74761 taaatgataa aagtaattta tcgatagaac aagcaaaatc aaaattaaat aagattgcta
    74821 atgatgtagg attggacact attaatgtag ctgtaaatga aaactatata aatgctttaa
    74881 aaccagatat ggaaacaatt atgggtggag tatttgtagg gttagtaatt gtattgtcta
    74941 gtattttagt aatatacaat atattttata tttcaatagt tacaaaggtt caagaatttg
    75001 gaaagcttag agcaattggt gcaactaaaa aacaagtaaa aaatatagta tttaaggaag
    75061 ggttcatatt agccggaata gcaataccaa taggaataat tttaggctat gtacttgcaa
    75121 atattataat aaaatcattt atggatatag atgtaaaatc atcacgatta ccagttatat
    75181 tatcggttgt ggtaataagc tttatatctg ttgttttatc acttttaaaa ccaatgaaag
    75241 tagcatcaaa agtatccatt gttgacgcag taagatatac aggaaataaa ataagtaata
    75301 agagtaagag aaaaggatat aaaaatataa atctaaagag attatcacat gccaatttag
    75361 aaagaaataa aaaacgtact tacatgacat tggcatcact tatacttagt ggaactatat
    75421 ttataacagt atcaactgcc ttagaaagtt ttgatgctga gaaaatggca agagaacact
    75481 ttccttatga tatagaagtt agactatctg gttatgaaat gaacagtgat aaaaatccta
    75541 aagataattt gaatatttta caaatggata atcctctaag taaagatttt tttaatcaaa
    75601 taaagaacat ggaaggaatt aaaagaatag aatctgcaag aagtgtaaaa attggtatgg
    75661 aggactatga tgttgaattt aagtatgacc ttttacaaag tataaatgaa aatgatgtaa
    75721 aatcattaaa caagaatata atagatggaa aaataaatct tgaaagactt caaactggag
    75781 atgaaatagt aattactcat gttgatacag caaaagaaat gggagtaaaa gcaggagata
    75841 aaataagatt aactttatat gatggagata aaaagataaa aaaagaattt aaagttcaag
    75901 caatagctat gggggttcca agttttggta tagggaagga ttttattgat agaactttga
    75961 aatacgattc aactagtagc ttgggaatat atacagaaga aggaaagtat caagaaatca
    76021 aagatagtat caagaagatt gctaaaaata atggattttt agagactgat tttatagata
    76081 gtagaattga gtcaaataag gctactattt catttataaa gataatggga tacacgctaa
    76141 caggcataat aggtgtgatt ggatttatga atcttgtcaa cacaatgata actagcatag
    76201 ttactagaaa aaaagaactt ggtatgcttc aagctattgg tcttactaat aaacaattag
    76261 tgaaaatgct taattcagag gcaatgtatt acacttctgg aatgatgata ggaagtattc
    76321 tatttggagg tatattaggg tatattgctg ttatatttct aaaaaagact ggattatctt
    76381 atgcaacata ttctttgcca atagtaccaa tcttgttgat gattgtgtgt attcttatag
    76441 ctcaatttat aacaacttat ttgataggac gaagttttaa taaagaatct cttattgata
    76501 gagtaagata cagtgaataa aatacaaata tttatggtta gtttaactag caaaaaacat
    76561 ataaaaaaat atagttaaag agattcataa ttaaataggt taaagcatag tacagagcaa
    76621 acaatttaag cagatgtttg ctctgtattt tattatgtaa tcaataatat atctaatata
    76681 ctgatattat tcatgttaga aaatgtaccg gtacaattct aaaaaagtat aaaattgtac
    76741 catataaaat gaaaattttg tataataatc gtatagaaaa taaataggag ctgatatata
    76801 tgaataagaa gattaattat gcaccaggac caactgaaac tagggaaaat gtaagattag
    76861 caagagcaga aaaaacaaca aatccagata tagatattga ttttgttgaa ttttataaaa
    76921 agacatgcga gaagtttggt aatataattg gaacaaaaaa cgatgtttac atattgagtg
    76981 gtgaaggaat attaggtctt gaagcagcat gtgcatcact tacagaaaaa ggagatagag
    77041 tacttgtaat tgacaatggt atatatggag aaggttttaa agattttgta acaatgtatg
    77101 gtggggagta tgtgcttttt tcaagtgaat atactaaaag tatagatatt gatgaattga
    77161 aagcattttt agatgaggat agtaatttta aatatgcaac agtagtgcat tgtgatactc
    77221 caactggcgt attaaatgat gtaagcaaaa tatgtccatt acttaaagaa tatggaatat
    77281 taactgtagt tgattctgta gcaggtatgg taggagaaag attaagtgta gatgaatcta
    77341 aaatagatat aatattaggt ggttcacaaa aagctatttc tgcaccagca ggacttacta
    77401 tagtcggaat aagtcaagat gcaaaaaatt gtataaaaaa tagaaaaact gatgtaatag
    77461 gtttttattg taatttaaat atatgggaag gctattatga aaaaaaatac ttcccttata
    77521 caatgccaat aagtgacata atggggcttg ataaagcact tgataatata ttagaagaag
    77581 gaatagaaaa tgtattaaat agacatgaaa agatagctta tagtgttaga aaatctattg
    77641 aggaatatgg tctagaacta tttttagaag aaggatattc taatacagtc acagctataa
    77701 aaattcctga aagtatagga gctttagaac ttactgatta tatgcttaaa aattataata
    77761 cacttgttgc aacatcacta aatcaatata tggatactat tttaagaata ggtcatatgg
    77821 gagagaatgc aaatctaaat aaaatagaac atgtactaaa tgttttggat aaaagtttaa
    77881 gtgcattagg attcaaagga aatggaaatt tgttaaattt atttaataag tattattttt
    77941 aatagatagg gggatatagt atggaagtta cagctaagca aaaatttaca acgaaagaca
    78001 tggtggaaac ctcactatta atagcattag tatttattgc aactaaattt ataaacataa
    78061 gattaccaat atctataaat ggtgggcttg tgcatttagg aactgcaatg ttgttcatat
    78121 cagcaatagt atttggaagt aaaaaaggtg ctttatcagg agctattggt atgtcacttt
    78181 ttgatttgat atctgggtgg actttatggg caccgtttac ctttatagtt agaggagtta
    78241 tgggttattt acttggaaag attgcatggg caaatggtaa taatgggaat aattttttaa
    78301 tcaatgtaat aggaatatgt gtttcatcaa tttggatgct atttggatat tatgtaacag
    78361 aagttatact ttatggtaat tttatagtac ctcttacttc aataccaggt aatttaatgc
    78421 aagtattgat aggtcttata atagcgcttc ctatttctaa ggtattgaaa aaatgtataa
    78481 aataaatata aagtgaacta aaatttatga tagcataaat tatactgtat aatattagga
    78541 tggagtacat aattaaaaaa ttttagtata gaggttagtt cacaatgtta tcagttacaa
    78601 gatattcaat caaaaatgtt cgtttaaagc cgtgggtaaa gtttatttgg tattttgaga
    78661 caaaatcaaa tatactgtta aataataagt tacttccgac agacagtata gatattattc
    78721 tcaatctttc tgatgttatg gaatataaaa ttgaaaatca agactatatt gctagtaata
    78781 ttcattttaa tggaataagg gataaacatg gatttattat tcaacatgga aatatacgtg
    78841 ttatagggat ttctttttat ccatttggtc tctatccatt tttaaagata ccaatttcag
    78901 agtttaacag acaaattgtt gacctagaag cagtatcaaa actatttgca aaaaaattag
    78961 aagagtcatt aaatccaaca aaagcaatag aaaaagttgt tttatgttta gaagaagtat
    79021 tattatccat attagaagaa gatttaattt caaataaata tgtaaagctt ctaaattcat
    79081 ttatatatgt taatagatat agcaatataa aagcattttg tgatgataca aatataaata
    79141 ttaaaacatt ggaaagaatc tgtttaaaat atacagggta tacacctaag attttaaaga
    79201 gaatttacag atttaaaatg gcaagcaatc aacttattta taattataaa gatgatgaac
    79261 tttttgattt tatttatgaa aatgagtact atgaccaagc acactttata aaagaattta
    79321 aaaaattttc agggacgtca ccaattaaat ttagtggtga gaataagacg attaaagaaa
    79381 atataaagta cagctattta taattatact gtcgattttt tacaatacct ccaatttatt
    79441 ttttgatatg atataggaaa ataaattgga ggttttacat atgaaaatta ttacagaaat
    79501 agttgagttt aatgtggaag aaaatttatc aaaagaagag tttatagaaa ttgtaaatga
    79561 cttagaatta aaatttcatt ctattcaatc tggttttatt gatactgagc ttttatatga
    79621 tgaaaagtct caaaagtgga ttatgataca acattgggaa tctatagaag acctcaagag
    79681 tgtttctagt aaaatgttta aagattcttc aactgagaaa tttagaaaag ctttaaagaa
    79741 tcaaactgtt aagatgaata taataccaca aattaaaagt tggaaattat agaactgaat
    79801 gtaaaagata gttaaatagt atcaaactaa attagccaaa taacattttg tatttaaatt
    79861 gttatttggc ttttagtgtt tattttaata tgagtgttat atactagaaa aaccaacaaa
    79921 ctgttagagt gtggattatc ataatgtcat attcgggagc taaaaggctt aatactgatt
    79981 tgccaattgg tcaatccatg catgtccatc tggatatgct gttattaaag catttacaag
    80041 ccaatccctt tgctcttgtg ataatagtgg gactgttaca tcgaagtctt tttgataacc
    80101 aagcctatct atgtatattg atttgcaaaa caaaaccact tcgggagcca tatatgggat
    80161 attatctgat gttatcagtc tggccttatc catatctctt ataattcttt gattttggga
    80221 aaaaagaaat tttccatctt tctgtggatt atatatcaat tcaataaaat cgaactgtaa
    80281 ctgttcgtca cactgtatat tcattttata gttatcatcc tcgacttttt ttaattcaat
    80341 aaatgagcaa tcaggcttaa tcgcaaaaac acaaatttgc tctatatgtt gcttgttaag
    80401 gtcgataata ggtcgtaata aataattacc caagggctca tatatatccc atccttgatt
    80461 aagcatatac tgaatgctct tttctttatc tgtgtcagaa atccatatgt caatatcact
    80521 gtgtgggcga atatgcatat tcgcaaataa ttctagggca tatccaccac atattgaata
    80581 tgaaaagcca caatcattaa aaaagtttac acaatcttta attagtttgt tcatagcaac
    80641 ctccttttgc atgtactgaa ataaattaaa ttttttaaaa taccaactaa atgtttgttt
    80701 tgtgtaaata taaaactgcc gatattaagg cagttttata tttactattt agaaaattta
    80761 ttaacaaatt tttgatattc acacattatc ttatcattca taaaagaaat acccataaaa
    80821 gcagcttgct caccaaacaa agtaaaacgt tctatcatct gttgtggatt acctggataa
    80881 actgttggaa tacaccatgt gtttagaaga aaaccaaaca tttgagcagc aatctctgga
    80941 tttttaactt ccatagaatt atcttgattt ccttcttcaa tcaatgcttt tatccaaggc
    81001 acaatatcat ttataatctc atcaaactgc gtagctaaat acttaggatt acgcaatagt
    81061 gatgatgaca ttaaaaaaat gtcttgttta ttgtgctgtt cataagtatg aagcaaccta
    81121 tattgtaatt tttctaatgc ggtctttcct tcatcatttt ttacctcata cccataaaat
    81181 ccatcattat acaaccgact tgtgattgcc ttaattattt cgtcttttga tttaaagtga
    81241 tgataaatag cacctcttga aaggccatct aaattatcta caatgtcttg aatcgttgtc
    81301 tgctcatacc ctttttctaa gaataactta tgtgaaatat caagtatttt ttgtatagta
    81361 ttatcaggtt ctttttttct tcccaaaaaa ctcactcctc gaaacaatca tttggtatgt
    81421 ttatcttagc aaatacataa tgatttgtca atgtgtcttt ttagcatgtt gaacaggtat
    81481 attgccatgc tttcattatt ttattttttt gccttatact ggactatatt tgcagtttcc
    81541 taatatattt ggctcatatt taaataccat tatctaaaag cactattgct gtacgagagg
    81601 caataggcat tttagaagaa ggaggagttg agctaaagta tacatcataa tctcttaagt
    81661 tttctttttt tgtgtttcct ccaaactcta ccatatcagt atttaatatc gttttgtagt
    81721 ttgtattttg aaactttgaa atatcaactg attgtattag attagggtgg aaattaaata
    81781 taaataaata tttgccctta ctgtacacca aaatcttttt atcattatcc atatgtataa
    81841 gttcatgact attttggata aaaatatcac tgtattttgt aagttcgagc atagatttat
    81901 caaaatttaa taattgttta tactttaagt tgttatcttc tgaaaggctc cattgtcttc
    81961 tggcatactt atagctccaa ttgttgcctt ctcgtggaaa atcaatccac tcaggatgtc
    82021 caaattcatt tcccataaaa tttagatatc cttcacctgc tagagaaaaa gttattaatt
    82081 taattaattt aataagagat attgctctat tgataacatg gttatttgag tttatttcca
    82141 tattccagta catttccttg tcagctagcc aaaaaataat tgttttatcg ccaactaatg
    82201 cttggtcatg ggattcacaa tatcctatat ttttttctcc aggtcgtcta gtagtaagtt
    82261 cataccacat tttgcccaaa tcccagtctt catcagataa gttagagata gtttttatcc
    82321 aaaaatcagg tactcccatt gcgagtctat agtcaaatcc aattccgccg tcttttatag
    82381 ggatacacat accaggcatt ccactcatat cttctgcaat acttatcgaa ttaggtttaa
    82441 tttctttcat taattcattt gcaaattgga gatatgttat agcttcaata tctgtattca
    82501 tactaaaata tttttcataa ctatcaaatg aaactccaag accatggtta tgatataaca
    82561 ttgaagttac accatcaaac ctaaagccat caaaatggta ctcatttaac caaaatttta
    82621 tatttgaaag caaaaaatgt attacttcag gtttaccata gttaaaaagt tttgtacccc
    82681 aagcaggatg atttcccttt gaacctgagt ggaaaaattg atgttcactt ccatcaaact
    82741 catttattcc ttctaaagta ttctttacag catgagaatg aactaaatct aataaaacag
    82801 caatccccat tgaatgagca gtgtttatga ggttttttaa gtcttcagga gtaccaaacc
    82861 tagatgagat tgcataaaag tttgaaactt ggtatccaaa agatgcataa tatggatgtt
    82921 ccataatagc cataagctga atggtattgt atccagcttt ttttatctta ggaagtattt
    82981 tttcagtaaa ttcattataa gttccaatag attcgctttc agtagacata cctatatgac
    83041 attcatagat tagtggagaa gttatgtttt ttaagtcaaa atcattgtca gtccagataa
    83101 atggagtttt tggttgccat atttgtccat taaatccacc aaaatctttt tgaattactc
    83161 tttttatata taatggtata cggtcaaatg tttttccatt tgcagtaact tgtaccttta
    83221 cctctgattt gtgaggtaag ctattttgac caggaataaa tatttcccaa ttaccagacc
    83281 caatttttct tagaggatga gattttctat tccaattatt aaagtcacca attagtgaca
    83341 aactatctgc atttggagcc cattccctat agaagaatcc atcatttgtc tgatgaaatc
    83401 catagtaaag atgagcattt gcaaaagaac taaaatcttt atagtttttc aatgtttttg
    83461 atttcatatc ctcataatgt ttcattcgct tttttatatc agattcaaat ggttgtaggt
    83521 atacatcaat atctaaaatt ttaaatcttt tttgactttt cattgtattt ctcctccata
    83581 ataaatatta catctaaaaa ataatattta tctcatacta tcttctctgt ataattgata
    83641 tatattatat aatcctaata aaaagcttaa gccaccaatg attaatttca taatttacct
    83701 ccctagtatt tatacttata tatcgtattc atcaaaattt aaaagtttgt ttaaaactct
    83761 tttaataata tcataatcaa agcctttata ggaaagatgt tgagaaattt tttgatatat
    83821 tttcttttta tcttctttct ttactctttc ataccttttt tttgctagat acatagcatt
    83881 ttcaaattca gtgtccttgt ctaattcaga tactgcttca ttgattgtac ttctattgat
    83941 acctttatta tataaattct gcttaattct gtttttgcca catttattta ggttaacatt
    84001 tgtgttaaca attttttgag ctaatacact gtcatttacg agattataac ttttaagaaa
    84061 atctatgacc ctctcaatta tattttcatc aaattctgat gatagttttt cttttaattt
    84121 tttttcagat tggtcagcat gagataaaat atttaatgct ttatttttag cttttatata
    84181 catttcatca tctaaaatag gttttaattc ttcttcattt atttcattcc ctttttttaa
    84241 attaaatgta taaaccagtt ctttaaatat agcaataaaa aatttatcat ctacataaat
    84301 attaactcta tcatcatttc ttttttgttg ttctattttt gtaattatac ccataagtcc
    84361 tcctttttta tatgtaatta tattaacaaa agc
//
LOCUS       DAMJYX010000006        81146 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJYX010000006 DAMJYX010000000
VERSION     DAMJYX010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 81146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.006"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            223..600
                     /locus_tag="PC479_000425"
     CDS             223..600
                     /locus_tag="PC479_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2803014.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            593..1741
                     /locus_tag="PC479_000426"
     CDS             593..1741
                     /locus_tag="PC479_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2803015.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1758..2360
                     /locus_tag="PC479_000427"
     CDS             1758..2360
                     /locus_tag="PC479_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2803016.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2453..3475)
                     /locus_tag="PC479_000428"
     CDS             complement(2453..3475)
                     /locus_tag="PC479_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2803017.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3804..3986)
                     /locus_tag="PC479_000429"
     CDS             complement(3804..3986)
                     /locus_tag="PC479_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803018.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4299..4718
                     /locus_tag="PC479_000430"
     CDS             4299..4718
                     /locus_tag="PC479_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2803019.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4849..6690
                     /locus_tag="PC479_000431"
     CDS             4849..6690
                     /locus_tag="PC479_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2803020.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6717..8604
                     /locus_tag="PC479_000432"
                     /pseudo
     CDS             6717..8604
                     /locus_tag="PC479_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            8612..10612
                     /locus_tag="PC479_000433"
     CDS             8612..10612
                     /locus_tag="PC479_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803021.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10874..12133
                     /locus_tag="PC479_000434"
     CDS             10874..12133
                     /locus_tag="PC479_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2803022.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12194..13981
                     /gene="iorA"
                     /locus_tag="PC479_000435"
     CDS             12194..13981
                     /gene="iorA"
                     /locus_tag="PC479_000435"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2803023.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            13983..14558
                     /locus_tag="PC479_000436"
     CDS             13983..14558
                     /locus_tag="PC479_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2803024.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14611..15681
                     /gene="buk"
                     /locus_tag="PC479_000437"
     CDS             14611..15681
                     /gene="buk"
                     /locus_tag="PC479_000437"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2803025.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15899..16600
                     /locus_tag="PC479_000438"
     CDS             15899..16600
                     /locus_tag="PC479_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803026.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16660..17166
                     /locus_tag="PC479_000439"
     CDS             16660..17166
                     /locus_tag="PC479_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2803027.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17341..18363
                     /locus_tag="PC479_000440"
     CDS             17341..18363
                     /locus_tag="PC479_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2803028.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18510..18818)
                     /locus_tag="PC479_000441"
     CDS             complement(18510..18818)
                     /locus_tag="PC479_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2803029.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18878..19306)
                     /locus_tag="PC479_000442"
     CDS             complement(18878..19306)
                     /locus_tag="PC479_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803030.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19529..20986)
                     /locus_tag="PC479_000443"
     CDS             complement(19529..20986)
                     /locus_tag="PC479_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2803031.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21404..22318
                     /gene="nadA"
                     /locus_tag="PC479_000444"
     CDS             21404..22318
                     /gene="nadA"
                     /locus_tag="PC479_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2803032.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22331..23632
                     /locus_tag="PC479_000445"
     CDS             22331..23632
                     /locus_tag="PC479_000445"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2803033.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23613..24449
                     /gene="nadC"
                     /locus_tag="PC479_000446"
     CDS             23613..24449
                     /gene="nadC"
                     /locus_tag="PC479_000446"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2803034.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            24705..25037
                     /locus_tag="PC479_000447"
     CDS             24705..25037
                     /locus_tag="PC479_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2803035.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            25199..25384
                     /locus_tag="PC479_000448"
     CDS             25199..25384
                     /locus_tag="PC479_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803036.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            25411..26496
                     /locus_tag="PC479_000449"
     CDS             25411..26496
                     /locus_tag="PC479_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2803037.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            26489..26947
                     /locus_tag="PC479_000450"
     CDS             26489..26947
                     /locus_tag="PC479_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803038.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            26982..28073
                     /locus_tag="PC479_000451"
     CDS             26982..28073
                     /locus_tag="PC479_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2803039.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            28078..28302
                     /locus_tag="PC479_000452"
     CDS             28078..28302
                     /locus_tag="PC479_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803040.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            28312..28875
                     /locus_tag="PC479_000453"
     CDS             28312..28875
                     /locus_tag="PC479_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803041.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            28836..29465
                     /locus_tag="PC479_000454"
     CDS             28836..29465
                     /locus_tag="PC479_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803042.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            29505..29642
                     /locus_tag="PC479_000455"
     CDS             29505..29642
                     /locus_tag="PC479_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2803043.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            29642..30394
                     /locus_tag="PC479_000456"
     CDS             29642..30394
                     /locus_tag="PC479_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803044.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            30407..30892
                     /locus_tag="PC479_000457"
     CDS             30407..30892
                     /locus_tag="PC479_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803045.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            30899..31048
                     /locus_tag="PC479_000458"
     CDS             30899..31048
                     /locus_tag="PC479_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803046.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(31184..32005)
                     /gene="yidA"
                     /locus_tag="PC479_000459"
     CDS             complement(31184..32005)
                     /gene="yidA"
                     /locus_tag="PC479_000459"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2803047.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            32498..34192
                     /locus_tag="PC479_000460"
     CDS             32498..34192
                     /locus_tag="PC479_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2803048.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      34524..34634
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            34738..35109
                     /locus_tag="PC479_000461"
     CDS             34738..35109
                     /locus_tag="PC479_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2803049.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            35204..36151
                     /gene="trxB"
                     /locus_tag="PC479_000462"
     CDS             35204..36151
                     /gene="trxB"
                     /locus_tag="PC479_000462"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2803050.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            36255..36572
                     /locus_tag="PC479_000463"
     CDS             36255..36572
                     /locus_tag="PC479_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2803051.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            36841..38127
                     /locus_tag="PC479_000464"
     CDS             36841..38127
                     /locus_tag="PC479_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2803052.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            38239..38712
                     /locus_tag="PC479_000465"
     CDS             38239..38712
                     /locus_tag="PC479_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38371..38373,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2803053.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            38744..40054
                     /gene="grdB"
                     /locus_tag="PC479_000466"
     CDS             38744..40054
                     /gene="grdB"
                     /locus_tag="PC479_000466"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:39791..39793,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2803054.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            40310..41842
                     /locus_tag="PC479_000467"
     CDS             40310..41842
                     /locus_tag="PC479_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2803055.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            41853..42998
                     /locus_tag="PC479_000468"
     CDS             41853..42998
                     /locus_tag="PC479_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2803056.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            43308..44387
                     /locus_tag="PC479_000469"
     CDS             43308..44387
                     /locus_tag="PC479_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2803057.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            44500..45099
                     /locus_tag="PC479_000470"
     CDS             44500..45099
                     /locus_tag="PC479_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803058.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(45285..46157)
                     /locus_tag="PC479_000471"
     CDS             complement(45285..46157)
                     /locus_tag="PC479_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2803059.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            46574..47977
                     /locus_tag="PC479_000472"
     CDS             46574..47977
                     /locus_tag="PC479_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803060.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            48004..49548
                     /locus_tag="PC479_000473"
     CDS             48004..49548
                     /locus_tag="PC479_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2803061.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            49579..50970
                     /locus_tag="PC479_000474"
     CDS             49579..50970
                     /locus_tag="PC479_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2803062.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            51163..52659
                     /gene="abfD"
                     /locus_tag="PC479_000475"
     CDS             51163..52659
                     /gene="abfD"
                     /locus_tag="PC479_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2803063.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            52794..53069
                     /locus_tag="PC479_000476"
     CDS             52794..53069
                     /locus_tag="PC479_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803064.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            53093..54400
                     /locus_tag="PC479_000477"
     CDS             53093..54400
                     /locus_tag="PC479_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2803065.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            54420..55538
                     /locus_tag="PC479_000478"
     CDS             54420..55538
                     /locus_tag="PC479_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2803066.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            55825..56745
                     /locus_tag="PC479_000479"
     CDS             55825..56745
                     /locus_tag="PC479_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2803067.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            56860..58017
                     /locus_tag="PC479_000480"
     CDS             56860..58017
                     /locus_tag="PC479_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2803068.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(58102..59601)
                     /locus_tag="PC479_000481"
     CDS             complement(58102..59601)
                     /locus_tag="PC479_000481"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2803069.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            60197..61621
                     /locus_tag="PC479_000482"
     CDS             60197..61621
                     /locus_tag="PC479_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2803070.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            61656..63785
                     /locus_tag="PC479_000483"
     CDS             61656..63785
                     /locus_tag="PC479_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2803071.1"
                     /translation="MKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDVVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            63815..64237
                     /locus_tag="PC479_000484"
     CDS             63815..64237
                     /locus_tag="PC479_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2803072.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            64256..65407
                     /locus_tag="PC479_000485"
     CDS             64256..65407
                     /locus_tag="PC479_000485"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2803073.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      65513..65614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            65797..66369
                     /locus_tag="PC479_000486"
     CDS             65797..66369
                     /locus_tag="PC479_000486"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2803074.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            66433..67083
                     /gene="fsa"
                     /locus_tag="PC479_000487"
     CDS             66433..67083
                     /gene="fsa"
                     /locus_tag="PC479_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2803075.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            67235..70159
                     /locus_tag="PC479_000488"
     CDS             67235..70159
                     /locus_tag="PC479_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803076.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            70748..71206
                     /locus_tag="PC479_000489"
     CDS             70748..71206
                     /locus_tag="PC479_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2803077.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            71267..71542
                     /locus_tag="PC479_000490"
     CDS             71267..71542
                     /locus_tag="PC479_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803078.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            71601..72875
                     /locus_tag="PC479_000491"
     CDS             71601..72875
                     /locus_tag="PC479_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2803079.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            73005..74057
                     /locus_tag="PC479_000492"
     CDS             73005..74057
                     /locus_tag="PC479_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2803080.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            74057..75088
                     /locus_tag="PC479_000493"
     CDS             74057..75088
                     /locus_tag="PC479_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2803081.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            75180..75992
                     /locus_tag="PC479_000494"
     CDS             75180..75992
                     /locus_tag="PC479_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2803082.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            75995..76915
                     /locus_tag="PC479_000495"
     CDS             75995..76915
                     /locus_tag="PC479_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2803083.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            77090..77536
                     /gene="rpiB"
                     /locus_tag="PC479_000496"
     CDS             77090..77536
                     /gene="rpiB"
                     /locus_tag="PC479_000496"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2803084.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            77566..78231
                     /locus_tag="PC479_000497"
     CDS             77566..78231
                     /locus_tag="PC479_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2803085.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            78341..79843
                     /locus_tag="PC479_000498"
     CDS             78341..79843
                     /locus_tag="PC479_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2803086.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(80155..>81146)
                     /locus_tag="PC479_000499"
     CDS             complement(80155..>81146)
                     /locus_tag="PC479_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2803087.1"
                     /translation="ISIVVQAIIQYLVLIFFMYIYYAEAIAIVILFTLVNTVFHFYIY
                     NFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDAN
                     IKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKL
                     LIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFT
                     MIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEEL
                     IERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT        32075 a         8838 c        14643 g        25590 t
ORIGIN      
        1 taaatagttt taatgtgtaa ataacattct acactattgt aattaaatat gtaaaaagta
       61 aaatatttaa atagatatat tttaaaatct aacttttaat attgtaaaaa aattagaaat
      121 ttaacaaaaa gtacaaaaat tcacattttt ttcataaagt gtttgactaa aaaattcaca
      181 tgttgtagaa ttaaggtagc aggagttttg agggaggtgt aaataatgat aagaaaaata
      241 ccacaagcag aactaaaagt aatgaaattc atttggaaag tagatgttac agtgacatca
      301 aaagatgtta ttgaagctat ggaacaaaaa tatggctgga aacaaaccac cacactgaca
      361 cttttatcta ggctggtaaa aagaggattc ttagatgctc aaaaaataga cagatataca
      421 cattacacaa taagagtaaa acacaaagat tatttaagtt ttgagacaaa ggatttccta
      481 agtaatatac atgatgactc tttaagaagc ttaatttcag cattacatga tgatgaaaat
      541 ataggtaaag ataagctaga ttttctagaa agttattttg ataatttaaa ggatgaataa
      601 ttatatgcta tatgtttttg aaaatcttat aaagacaact attgtatgta gtcttggtat
      661 ttgtctatta ttatttttga aaagatattt atttaagaag tttagtaaga gattcaatta
      721 ttatatttgg ttgataatag tctttagaat gttacttttt ctatttaatt atacaatagt
      781 gtatgaagta aaagaaccca aagaaaatgc attaggaaat aatattacac aaattgatat
      841 ttctacagat aataatctta tgctgtatgt agcttattta tggttatttg taactatagt
      901 cattgcagtt tatacattta taaagtatac aagatttaaa aatctagttg ttgatgtatc
      961 ttatgatatt gaagataatg atataaactg tatctacaaa aacttgttaa aagaattaaa
     1021 tataaaaaag aaaattgaat taagaggttc tgatgaattg ataagtccag caggtatggg
     1081 attatttaaa tcttacatat tcttaccaga ctatccttat agtaaagatg aactaacttg
     1141 gattttgaaa catgaattaa tgcattttaa aaataaagat attttaataa agtttttagt
     1201 tttgtctgtt agaataatat attggtttaa tccacttgta tatgtaatga gcaataaagt
     1261 aaatctagat tgtgaattat gttgtgatga aagtgttttg gtagaatgtt ctttaaaaga
     1321 taaaaaagaa tatgcactag cacttataaa gtcaattaag tttagtaaaa attataatag
     1381 taagattttg acaaccgagt ttgataagac aaatttggaa aagagacttg agagtattgt
     1441 taaaaagaaa ggcaaaagtg gaatactaat tgctgtttta gtatttatga cttctataac
     1501 atcttttgtt gaagttgatg ctcaagttag aattgatgag ggtaataatg gaatgaataa
     1561 caatattaat gaatttttat tgaataagac gactgataaa attataaata tttattataa
     1621 agatgcacca aatgaaatta ggaaatttta tgaagataaa tgcgaattag aaggtaaaat
     1681 acctagagac tatgacataa tagaaatgga tatgaaagat tataatcaat taataaaata
     1741 attataagga ggattttatg ttaacaaaat taagaaaaat agtagctatt atgtgttgct
     1801 tattgcttac ttttacaagt gtggcatttg ctgacacaag taaagagaaa ggaggaagta
     1861 aagagactgt tccagagaaa aacctagatt cttttactct ttctgggtat atatatacat
     1921 atgaaaatgc gccacaagaa ataagagatg aacataaaca aaattgtgaa gagataaata
     1981 tagaccctaa gccagatgat gagatatttg ttccagaatc agctaatcta atgaatgagg
     2041 acaatagtaa aggtgtttat gcaagttata ctgtttctta taatataggt gctaaaacta
     2101 taacaattat gagtaatgag tatttgacta taagtacaac aaaagttata cgaaagggta
     2161 atagcggaaa agaagtaaaa gctgcacaaa tcatgttaac tttacttggt tataatgtag
     2221 gtatagatag ttcttttgga agtaagacat ataatgcagt tgtaagtttc caaaaaaaat
     2281 acggtttgtc tgcagatgga ataattggtc cagctacttg ggataaattg ggtagactaa
     2341 ctgaccctac tctttcatag gattaaaatc taaaaatata ttaaatagtg tctcaatatg
     2401 atttttcaat ttaaatgaga cactatttaa atataaatgt tataaaacta ttttacaata
     2461 aattccatat aactctacca tctatatcct taacattttg acaaccagtc aaaatcatag
     2521 tctcacataa ctcattccta accttctcta taaaaaactc cacaccatca agaccaccgc
     2581 caaaagaagc agtaacaaaa ggtcttccca tcaaaacagc atcagcaccc aatccaagca
     2641 tcttaactac atcaacacca gtcctaactc caccatcaac aagtattgtt atctttccct
     2701 ttacagactt agctatatct ggaagtacct cacaagtacc aggagtataa tcaagtactc
     2761 ttccaccatg atttgaaaca ataatggcac tagcaccaga ctctacagcc atcaaagcat
     2821 catctacagt cataattcct tttataataa aaggtaatct agtggattca accaattctc
     2881 taatctcatc aattgtttta ggagaaaaag gattttcttg aaattgatta ttaataagtc
     2941 cacaagcatc aaggtctaca ccaactgcaa aagcacctgc ttcttcagac aatctaattt
     3001 tatctattat ctttgaatta ttccaaggct ttataaatac aattccattt cccctattat
     3061 ccttcaatac atctaaatta tccagtaaaa aagtatctac attagtatcg ccaaccatag
     3121 cataaattcc agaattagaa caacctctaa caacaggctc tatgtattct ttttcggaaa
     3181 ctttaccacc catgttcaat atagtacctg atacaggtgc agcgaatata ggagaactca
     3241 tttttctacc aaataattct atggatgtat ctggctttga aacattatgt atgactctca
     3301 tatttatctt tattttttct agactttttc tattttcaat gaaagacgaa ccacttcctt
     3361 taccacccat accaggaaca tctccagcac aagctaatcc attacaaatt ttacatactt
     3421 tacatgttcc attaaagttc tctctagctg attttagtaa gtcattgtaa tccatacaaa
     3481 atctctcctt tttataatat taaaattgag tattactata ttgaaaatat cttaattgta
     3541 aatataggga tgttattatg atgagctaaa ctagataata catattagat attttttatt
     3601 gttgtagata gttaaattat atcatattag atagtcaaat tttgaaaaaa actaacttat
     3661 ttaagtatac attttgaaaa aattaaaaaa gctatatata gatttttaaa gaaatctata
     3721 tatagctttt aagtttatgt caatcttttt tgttaatgaa taaataatat agaatacaat
     3781 atacaaaaaa gtttcttgaa atgctaagat aatccccatt tacaaagcaa catcattata
     3841 ctgtgtaaat ttgaatcaac aatgttttca ccatatttca tcataaaatc ctctaattgt
     3901 gtgtcaataa atgaataata tccacttata ctatcgatgt atgcagtcat taagtgtata
     3961 cattcatcac ttttatcttt atacatatat atctccccct atatacaata tataaattta
     4021 gataccaaat cgtatcatat taaatagtta agtgtgtaac atctatgcag ttatatattt
     4081 atatcattat attaataact aaagtgagtt ttgacaatag attttgggaa actggatgtt
     4141 caaagtgcaa acttggattt taaaatcatt aattaagata gcgcatagga atatatgtat
     4201 acataactta attataaaga ttataaacaa ttacttttat taactttatt taaatggtag
     4261 aataacttgg agattaataa ttataaggag gatataatat gagtttatat aagtttatat
     4321 atgatgataa ggaatattta ttaaaagagg agaactgtag tgctttaata aatgatgaag
     4381 aaaatccagt tcaaggagta agtatttcaa aaataattga tatacttaat gaagcagaag
     4441 aagttgattt tgatgtagaa tattatcaag aagcatgtcc attatgttta gaaggagtaa
     4501 aagaaaagaa aaaattcttt ccttttttag aatatcactt ttatattttc agtaaagatg
     4561 gagaatatgt aataagcaat atatctgttg attataaagg actatctttt aataaactat
     4621 caagggcaaa taaagttgat aatagttata ttgtaagtgt gataatttgc gaaaattgtc
     4681 aggattatat agttcagata gaaaattgta tagtttagtg taaagtatgt cttaatttaa
     4741 gagaaatttc taaattgaga catcaacttt tttcaaaaat agtatggaaa attactaaat
     4801 ttatgagata atgtaagtcg gaggtgagtt acaatatata gtaaatattt ggaagagata
     4861 gatgatacaa ttttagattc aatgtttaaa ctaaccttga atgaaagatt attgactaat
     4921 aacgatttta ttgaaaataa aattgatgta gtaaagaatt taattgattg ctataaaact
     4981 gaacaaaata gtttaatgtt attaaaatca aattttttgc tagctttatt atatggtatt
     5041 caagggttta gtgaaaaaat gaaagagtat attttgattt cctgtaaata tattgataaa
     5101 tgcttaccca aagattataa atttttagca cgattttatt cacaaatagc tattttaagt
     5161 ttaaaaaatg aagatgtaaa taaggcaaat ttatatatgg ataaatttaa tcttatttgt
     5221 gatgaaaata attttctaat tgaagaaatt atttttaaat cacaacttat ttacattaag
     5281 gcaagtaagt gtatagaatc ttctgtaata attaaagaaa tagaaacgtt gtatataaag
     5341 ataaaagaaa ttaacgattt taattgtaaa aaaatatatt tttttattat tggtaaaata
     5401 tattttttgt ttttggatga tgctctaatt gctaaggtaa attttttaaa agcaagaaaa
     5461 tatgcagatt tactaaagga tatgcaagta tcttcactat gtaatataaa gataggtgag
     5521 tgtgaatgtt cgtttgaaaa ttatgaatgt tccatggaat atttcaatga agtaataagc
     5581 aataaaaagt atagaaatgt aaatataatt caaaaatata gagcatcaaa caacataact
     5641 aaaatattaa taaagactag aaattatcct gtcgcaataa ataatttagc aaaatctgaa
     5701 gtatatctag aaaaaattaa aaatcatagc ttaaaagaag tagagaagtt tgatttattt
     5761 ataagtttag ccatgtatta tgcaaaaagt aatgaaaaat cttttgaaca atcaatatgc
     5821 tatttagata gagctaaaaa catattaaat atgataaaaa atatagaaga atatataata
     5881 tatgatttag aaataattta tcaccaggca aagatatatt atatctttgg atattatgac
     5941 aatgccttaa atacaaataa aaaactcctt gaaaaatcta aaaatgctaa aaattataat
     6001 tatgtgaagt tagcatacaa gagtatatat ttgtgttttg aaaagattca aaattatgaa
     6061 atgagtatga aatactttaa aatgtattat gagttaaaaa tgatgtatat aaaagctcga
     6121 aatagaaatt atatagattc cttaaattat agacatgagt atatagaaaa agaaataaat
     6181 aacatgagaa aagtaaaact tgatttagat aaaaaaaagt atgttgacca tttgacaagt
     6241 gcatataata gaactttttt gaacgatttt ttagaaaaac aagaagttag tgaatattgc
     6301 acagcattta tgatagatgt agattacttt aaagaataca atgatactta tggacattat
     6361 aatggagata ttgctcttaa aaatataact ataataataa aaaaatatct aaaaaaagac
     6421 atgcttatga taaggtatgg tggagaagaa tttctgatat tatctgtatg taaagattat
     6481 aaaaaaagta aaatatttgg aagaaaatta tgtaggattg taaagaaatt tttaaataat
     6541 aatttgacag ttagtattgg cattgataca tgtaaaaata gtagtataga tataaaagaa
     6601 ataattgaaa atgctgataa agctttatat aaagcaaagg aaagtggtag aaatagatgt
     6661 ttgcattatc atgattttaa gaatttttaa ctgttgaaat aggattggtg atttacttga
     6721 aaaggagttt aatagaagat ttaaagagaa atataaacaa gatagaatac gatatgttga
     6781 ctaaattcta tctaaatttg acagatttaa actacatctt ggattatcac aaagaccttt
     6841 taaatacaaa attagaagat aatagtagag tctatacttt atatttaatt agtcttataa
     6901 actatataaa aaaagataga gaagcatcat acaactattt gatagagtca ttaaagtata
     6961 ttaaaaaatt gtcattaaaa gatgattcag atataatatc taaaatttat atttatctta
     7021 ctttatcagc tatttctata agaaaatatg ataaggtaaa taatttttat ttctcagcaa
     7081 aaaaaattat aaggcaaaat catttaaatg agttgttggt attgttaaat atgagtttat
     7141 gtgtggaaat aagtgcttta tatcagacca aaacggatgt aatagcttta gtagaagaag
     7201 tgtctttttt taaaacttta aaaaataaag ctcttgtaag tagggcaaat tatatatttg
     7261 ggagagttta tctatattta tttaataact ttataaaaag tatggaatac ttaattgaat
     7321 gtctaaaatt ggctcaagaa aataatttat actctataga ggctttttgt aaatgtataa
     7381 taagtttgtg ttatttagag agtggaaata atgtagaagc tataaaatac ataaataatg
     7441 tattagatgt tgcacacagt aataactcaa tatcagtaac tgaaagagtg tctataaaaa
     7501 tagaccttat atgggcatat ttaaaagaag atatgaataa agaagcagaa gttattttat
     7561 taaagactat aaaattaatg aaagttgttg aaggtgtata taaggattat ttatattcat
     7621 ttatattttt atattcagct gaaattgagt taaagaaaga taattgtagt tttgaaaagg
     7681 taaacaccta tttaatttta tcaaaaaata tatataaaag ttttggagat gtttttatat
     7741 ataattctaa actagattac atatataagt tgtatggaga tttatatata aaatttggta
     7801 atatagatga aggtataagt tgttatataa aaggtttaaa tttagttaaa aagagttcat
     7861 tgaatcttag aaaaatatct atatttatat gggctaatag caaatggata tgagctaaaa
     7921 aaagatttta aactggcaat agaatattat aaaagtatgg attggtattc tgataggtgg
     7981 gaaaaaaata attcctataa aacatctcaa gcaattcaca aacaatatga actcagacaa
     8041 aaacaagaga gtttaagatt actagtggat gataataaga aattagaaaa tgatatttta
     8101 aaagatggac ttactaaact atataataga agatatttgg aacagcagtt ggatttattt
     8161 aatggaacca gagacaaaaa tatggtcgca atactggtcg atatagattg ttttaaaagt
     8221 tataatgata attatggtca tctagcaggg gataaagtta tagttaaggt aactgatatt
     8281 ataagaagtg tatttataga tattaccgaa cacataatca gatatggggg agaagaaatt
     8341 ttaatattag tagattctat agaatcaatc ggtgttgaag agattgataa tatagaatta
     8401 tatattaaaa gagttttcaa acttcttgaa tttgagggta ttgaacatat atattctaaa
     8461 ataaaaaact atataactat aagtgctgga gtaagtataa aaaggtgtcg tagtagaaaa
     8521 gatgttgaaa tattaattga agatgcagat aagaacctat ataaatcaaa aaaagcaggt
     8581 agaaatcaat acattataat ttaaaattga ggtgattttg ttgcgaaaaa aaataggaat
     8641 tatagcctct gatattgaat taaaagaaag aatagaagaa ttatatagag aagatgtaga
     8701 aaatggaact ataataatcg acatacttaa cctagactta atggaaaatc aaggtcgtat
     8761 acttgttgaa aaaggtgccc aagctataat tggtagaggt ggaggttata gcttagtaat
     8821 agatacagta aatgtacctg ttataccaat gaatatgaag tcaactgacc ttttaagagc
     8881 aatagaaata gcaaagaaat attctaaaaa agtagtattg attcttggag acaatgaagt
     8941 ttcttttgac tatgttggct ggagaaatgt cataagtact gaaataactg aagaatggtt
     9001 tgaatctaag tatgagataa gaagtaaagt tgtaaaatat atagaccaaa aagatgaagt
     9061 tgtaatcgta ggaggaggtt tggcatgttc ttttgcaagg caatatggaa ttgatagtgt
     9121 atttgcaact gctagtgatg aatctataag agaagctgta gaatattgta aaaaattatt
     9181 agatacatta ggtgaagaaa agtttaataa tgaagtgtta cgaaatatac ttgatggtat
     9241 aaaagatgga gtaattgcta tagatagtaa tggcagtata attttgtaca atgaaagtgc
     9301 aaaaaacatg cttaaagtag agagaaaatg tgcacttaat aaatacatct tagatgtatt
     9361 tcctaaaatg gagtggatgt tagattgttt acatgaaaaa gaggacgtag aagatagaaa
     9421 gataagaaat ataaataatc taattgttaa tactaggaca acattgataa aagttgataa
     9481 tagtacttat ggagttttag gaataataca agatataacc aagctacaaa acttagaaag
     9541 aaaaataaga tttgacttga atcaaaaagg tctctatgct agatatactt ttgatgattt
     9601 tttatttaaa gataaattaa ctaaagaatt tattgaagaa gctaaaaaaa ttggaaaaag
     9661 tgattataca actttacttt atggagaaag tgggtctggt aaagaaatta tagctcatag
     9721 tatacacaac ataagtaaaa gaaaagatag gccttttgtt gctataaatt gtgctactat
     9781 agcagaaaat ttacttgaaa gtgaactttt tggctatgaa gaaggtgcat ttactggtgc
     9841 tagaaagggt ggaaaaagag gactatttga acttgctcat ggagggactc tttttttaga
     9901 tgaaataaat agtctatcct ttaatattca aacaaaacta ttaagagtaa tagaagaacg
     9961 tcaaataatg agaattggtt ctgattatat aatacctctg gatataagga ttatagcagc
    10021 aacgaatgaa tctttaaccg aaaaaatagt tatgggtaca tttagagcag acttatttta
    10081 tagattgagt agtttagaga taaatatacc tcctttaaga gacagaagag aagatataat
    10141 acctttgttt aacaattttg taaatgaagt attaaaggat gatggtctca atggaataaa
    10201 cagtatagat gaaaattttg ttttgactaa agatgaaatg gataagctat ataattatag
    10261 ctggcctgga aatgtaagag agttaaagac aatagctcaa aaatatgttg tgacaggtaa
    10321 aattaaattg agacaagata gagattttaa aactaaaaaa ttacttccta actctgaagt
    10381 agacaaattt aattctgaaa cgacagcaag tgtagaagtc caagatgaat ctataaatat
    10441 aagtaagata aatgatggta agataagtat agatataaaa gaagtaaata agtatgttga
    10501 agaaaaaata ataagtatgc tttttgctca gggactttct aaaaatgaag ttgcacaagt
    10561 tcttggaata agcaggacat ctttatggaa gaaatacaat aaaaatattt aaatataatt
    10621 acatataaat tattttgaag taatgttaat tttttaaaac aagtgttaag aaaaattaac
    10681 attacttttt tgcaaatttt tattttttta gacaggcata tatatttata agaaaaatag
    10741 ttaaaacact taatttaata atgaaaaaaa taataattct aaacaaaatc aattttacat
    10801 aaagtgggat ttattggcat agtaattgct taatatataa tgtaagagta aatacaattt
    10861 taggggtgat ggtatgagtc aaagtatggt tggtaagcat gcaatgtggc caaaagaaaa
    10921 tgatgttata tttagcatat ctggcagagc tcaagcagca gagaaggctt ttggtatgga
    10981 taatgtaata aatgcaacaa taggcgctct aatggatgat tcaggaaaat tgataacaat
    11041 gaaaactgta tatgaagaat ataaagcatt agataactgt gagataggtg catatgcagc
    11101 tcttgaaggt caaccagatt acttagaagc agttaaaaaa gtatttttca gagattactt
    11161 accagaagga catataagag tattagcttc accaggagga agtggtgcaa taaaattagc
    11221 tgtatggaac tatactaatg aaggagatga ggtattaaca tctgattggt tctggagtcc
    11281 atatgtaagt atagctgaag aggctaatag aaaagtagtt aattaccaac tttttgatga
    11341 aaatagaagg tttaattttg agtcgtttaa agaaaaattt gtaaatatag cagaaaaaca
    11401 aggtagagtc tttacaatta taaacacacc agcgcataat ccaactggat atagtgttgc
    11461 agatgatgag tgggataaaa tattagactt atcaaaagaa gtcgcaaaag ataaagacaa
    11521 aaagataata tttttcgtag attcagcata tattgacttt gcaggtgatg atgatgtatg
    11581 tagaaagttt tttaagaaat tttctaattt gcctgaaaac gttctcgtat tggttggatt
    11641 cagtatgtca aaaggattta cagcttatgg tatgagaatg ggagctataa tttgtatatc
    11701 ttcaagtgaa gatgtagctg aagagttcca ttactcatgt gttcattcat gtagagcaaa
    11761 ttggtcaaat tgtaatagaa gtgctatggc agtattatct aacattgtaa atgaccctaa
    11821 aaaattcaaa gaatatgagg atgaaaaaga aatatacaaa aacatgctta ctagaagagc
    11881 agatgtattt gtaaaagaag ctgaaagagt aggattagaa attcttccat atatagcagg
    11941 tttctttgta agtataccat gtgataatcc aaaagaagta tgtgaagaat tgactaaaca
    12001 taatttattt gcagttccat taaaaatggg cttaagattt gcagtttgtg cagtttctga
    12061 agataaatgt aagaaagcac caagtattat aaaagaagca cttgaaagtt tagaagtaaa
    12121 aataaataat taatttcatt gaacaaattg ggtggactat gaatttaata attgtagatg
    12181 gagggaattt tatatgaaac aattgatgac aggtaatgaa gcaattgcac gtggagccta
    12241 tgaggctggt gttaaatatg catctgcata tccagggact ccaagtacag aaatattaga
    12301 aaatatagca acatataaag atgcaatagt tgcagagtgg gctccaaacg aaaaggtagc
    12361 acttgaggct gctatagggg gttcaattgc aggagcaaga acaatggctt ctatgaaaca
    12421 tgttggtttg aatgttgcag ctgatccaat atttacttat gcatatacag gggtaaatgg
    12481 tggtatggta cttattacag cagatgagcc gggaatgcat tcatctcaaa atgagcaaga
    12541 caataggatg tatgcaaaat ttgcaaagat acctttattt gaaccatcaa ctagtcaaga
    12601 agctaaagat atgataaaag aagcttttga agttagtgaa aaatacgata caccagtttt
    12661 atatagagtt acaacaagac tttgtcattc aaaaggatta gtggagtgct atgatagaga
    12721 agaagttgaa attaaagagt atgttaaaaa tgctaagaaa atggtaactg ttccagcaaa
    12781 tgctcaaata agaaggggcg ttgttgaaga aagaatggaa attcttaaaa aattctcaaa
    12841 tgaaactgat ttaaactact atgaaataaa tgatacaaaa ataggtgtta tagcatctgg
    12901 tatgtgttgt aattttgcca aagaagtttt tggaaaaaat gcatcatata tgaaactagg
    12961 atttacaaat ccattacctt atgaaaaaat aaaagaattt gcagaaaaag ttgataaaat
    13021 atatgttata gaagaaaatg acccatttat agaagaacaa ataaaagcat atggaataga
    13081 ttgtattgga aaagatgtaa taccaccata tggagaaatg acaccagatg ttttaagaaa
    13141 agctatattt ggaaaaacaa atgatactat agaatacaaa tctgaattag taacaccaag
    13201 accaccaagt ttctgtgcag gatgtccaca tagaggattc ttctatgaac ttggaaagag
    13261 aaaaaattta atagttggtg gagatatagg ttgttatact cttggatttg ctcctccata
    13321 taatggaata gattatgttg tttgtatggg ttctgctttt ggaactgcac atggagctca
    13381 aaaagttctt aatatgaagg atgataatga aaaaagattg gtaggtgtac ttggagactc
    13441 aacattcttc catacaggaa taaatggact tttagatgtt gtttacaata gaggtaattc
    13501 tatttcggtt atattagata atagaataac tggaatgaca ggtcaccaag aaaacccagg
    13561 ttcaggatat actttacaag gtgctaaaac taaagaagtt gatatagaag gattagttaa
    13621 agcttgtgga atagaacatg taagagttat aaaccctaat aatttaaaag aagtaaatga
    13681 agcactagat tgggctttag ctatagaaga tgaaccatca gtaataataa caagatggcc
    13741 atgtgtactt aaaaaattct ctaaagaaga tatagaagaa tttaataatc catttaagac
    13801 taagtgtaaa gttgaccatg ataaatgtat aggatgtaaa ttatgtttga aaactggatg
    13861 tccagctcta tcatttgata aagagaataa gttatcaaat atagatagaa atcagtgtgt
    13921 aggatgtgga gtatgtgcac aagtttgtcc aaaacaagca atagttaagg aggaaaaata
    13981 atatgactaa gagtatactt ttggtaggtg taggaggtca aggaactata cttgctagta
    14041 aattattaac tatgggattg atggaagctg gatatgatgt aaagatgagt gaaatacatg
    14101 gaatgagtca aagaggaggt tcagtttcct cacaagttag atatggagat tgtgttcatt
    14161 ctccagttat agaaattggt ggagcagata tattagtatc ttttgaaaaa atggaagctt
    14221 taagatggtt caattattta aaaccagaag gaaaagcttt agtgaataat cataggatag
    14281 attctatgac tgttttaatc ggaggagctg aatatcaaga aagtgatata gatgcagagt
    14341 taagtagatt aaatgcaaaa gttataaatg cagctgataa agctgaagaa ctaggaaatt
    14401 caaaaataat gaatgttatt ttattaggat gtctagttaa atctatgcaa ttagagtcta
    14461 ttgattggga aaaaattata agtgaaaatg ttaaaccaaa atttgttgaa ttaaacataa
    14521 aagctttcca tgaaggtatg gaaatggtta gcaaatagtt tataatagat ataaatagac
    14581 tatagaaatt aaggtgaaag gaagaaaatt atgacttaca gaatattagc cataaatcca
    14641 ggttctactt ctacaaaaat agcagtatat gatggagaag aacaaattct tgtgaagacg
    14701 atagaccatc cagctgaaga gattgcaaaa tataatacta tacaagacca gtttgaaatg
    14761 cgtaaggaag cagttttgaa tattcttaaa gaaaatagta tagacttaaa atctcttagt
    14821 gcaatagtag gaagaggtgg agttttacca ccagtaaaat caggagcata tttagtaaat
    14881 gaagaaatga ttgatgtact aagacataga ccagtacttg aacacgcttc caatttaggt
    14941 gctgttgtgg cacatgcaat atcagaacct cttggaatca actcatatat ttatgattct
    15001 gttgcagtag atgagcttat agatgtagcg agaatatctg gactttgtgg aatggataga
    15061 tcaagtgcag ggcatgcatt aaatactaga gcaatggctt taaaatatgc taaggataaa
    15121 ggaaaagatt ataagagctt aaacttaata gtagctcaca ttggtggagg agtaagtatt
    15181 tatcttcatg aaaaaggaag aatggttgat atgctatctg atgatgaagg accattttct
    15241 ccagaaaggt caggaagagt acctgctaca aaattagtgg ctgcctgtta ttcaggtcaa
    15301 tattcagaaa gagaaatgac taaaaagata agaggtaaag gtggtatagt ttcataccta
    15361 aatactgtag atgctagaga agttgaaaaa atgatagcag aaggaaatga agaagcaaaa
    15421 attatttatg aagcaatggc ttatcagtta gcaaaaggta ttggagagtt agcaactgta
    15481 gtagatggaa aggtagatgc tataattata acaggtggaa ttgcatattc tgaaatgttt
    15541 acttcaatgg ttaaaaagaa agttgagttt atagcaccag tagaaattat ggcaggagaa
    15601 aatgagttgg aatcacttgc ttttggaact ttaagagtac taaatggaga agaagaagct
    15661 agaatttata gtgaaaatta ataagaaaaa ctaaaaatgt ttatatacta aaaaagtggt
    15721 atatccgttt tttatacacg aaagcttaga gtataggtga aaatgtataa aatttggatt
    15781 gccacttttt ataattaaaa ttttatctta acctaataaa taattctgta aaatttcgta
    15841 tttatatata tattttattg gtatttattg ttattattat tatgaggagt gatttttgat
    15901 gggaaaaata agtttaaaaa gtatgataag attattttca ggttttttta tatttgcaat
    15961 ttcttctgtg cttatgatta atgcacatgt aggtcttatg ccttgggatg tacttcacca
    16021 aggtttatct ataaagctag gcataactat aggtcaggct tctataatgg taggagttgt
    16081 aattgttata ttggatgcag tttttggtga aaatatagga tggggtactt tactcaacat
    16141 gacatttata ggaattttta ttgatttagt aatattttct ggagtgatac ctcatgcaag
    16201 caacacttat ataggtgtgt ttatggttgt tataggaatt attttggcag ctatagcaag
    16261 ttttctatac ttaggtgttt gtttaggaag tggaccgaga gatgggctta tgattgctct
    16321 tcagaaaaaa actaataaat ctgtacgtct tgttagaaca attcttgaaa tactagcatt
    16381 agttgttggg tggttattag gaggttctgt aggtattggt acgcttgtaa gtgctttagg
    16441 tttaggatat gtattacaaa tagtgtttag aatatttaaa tttgacacaa aattgttgaa
    16501 acatagattt attatagatg atataagaga atggaaggaa aagaaaagca atgaacataa
    16561 atgtaaatca agtattgtaa taaaaaatga gcagaattaa ttatgctaaa atatatttgt
    16621 agtttatcta atttatagta aagtcaagga gattttgaaa tggaattatg ggatttatat
    16681 aatgctgatg gaattaaaac aggaaatgtt ataaaaagag gtaattctat tgaagaaggt
    16741 tattaccatt tggcagttga agtgtggatt ttaaatagta actctcaaat tttaattcag
    16801 aaaaggtcta aaagtaaaaa gacattgcca aatatgtggg gaatgacaac tggatgtatt
    16861 gtttctgggg aagacagttt agaaggagca attcgcgaag caaaggaaga aatagggata
    16921 gatataacta aagatgagat gaaagttttt agaagtatga ttcatgaaga tactctttgg
    16981 gatgtatact tagttaaaaa agaatatgat atatccaaag ctattttaca ggaggaagaa
    17041 gtaagtgata taaagtgggt aagcacagat gaaataagac aattactaaa agagggtttg
    17101 ttttttgaat atccagagat atacgaattg ctatatgaga tagataataa taaattaaat
    17161 atatgatgta gagcagattt cagtataagt aagattattt aaaatgtatc ttctctaaat
    17221 tttaatattg ttttatgaaa attaataagt tatttttagt atttttctac ttgttttttg
    17281 agattaaaat aaaaagggca gtcattataa agtcataaaa atttggggag gatttaaact
    17341 atggaagata acaaaggatt ggcacgtgac agttcaaaat ataaaattgt ttctatatct
    17401 gtcgtagtaa ttattctaat tacattttgg tatatgttaa atatggtgct tttaacattt
    17461 attatgactt ttattttcta taacttgctt gtagccacaa gaaaacgaat taagaagttt
    17521 tcttctttaa atattccaga ttcattaata attatagtgt tatatgcact atttgctata
    17581 ttgttagtat tgattagcta tgcagttgta ccaataatta tagttcaact tacagagttg
    17641 agtagggttt ttagtgattt tgatgtaaat caatttgctc aatctttagg accaaaattg
    17701 tatcctattg tctcaaagct ggattttaat aaatatattt cacaagcagg gctacttata
    17761 gcatcaacag caacaaaagt tggaagtttt ggagtaaata tattattggc atttttactg
    17821 agtttactcc ttttacttga aaaaaatgaa attaaaaatt ttggagataa actttctgat
    17881 agtaagattt cttttattta taattcgctt gtattttttg gaaagagctt tgttaaaaat
    17941 tttggtgaag taatgaaagt acaagttatg attgccttta taaattctgt tgtttctatg
    18001 attttcttag gtttcatggg attccctcaa atatgggctt taggatttat gatttttgtg
    18061 ttgggtctta ttccagtagc aggagttata gtgtctttga ttccattaac tgtaatagca
    18121 tttaatacag gaggaataac aaaggttttt ggagttttac ttatgatttg tatagtgcat
    18181 gcagtagaaa catacatatt aaaccctaag ttgatgtcga atagaactaa acttccagtt
    18241 tgttttgttt ttattatttt acttgtagga gaacattatt taggtgtatg gggattacta
    18301 attggagttc caatatttat gttccttatg gatattctgg gtgttaaatt tacttctagg
    18361 taatatatta aaataataat ttatctttat atataaatta taaattgttt taaaaaagaa
    18421 cctataaata gacatcatta ataaagacat ctatttgata ggttcaattt tgctttgtat
    18481 aaatactata aaactaaata aaaagaaatt tatttcattt caaaagtttc acaacttgca
    18541 tttctttcat ttatatgaac tttttcagct ttgcaaagtt catttttatt gtatttacat
    18601 tttacagctt cacaatgaat gttatctgta tcaacttggt tagtttcatt agaacgacta
    18661 gttataccag atgtatattt gtcttcaaaa gaagcacaat aagtattttc tgtagtagtt
    18721 gcttgcattc catctacttt tatactacca gcataacaat atccactagt attataagca
    18781 caattagtag cagcacaatt taaattatta tttttcataa ataacctcct aagttttatt
    18841 taaacttatt atgtgaagtt atctaaattt tatacactta tttacaatta catttatcac
    18901 atttaacaac tacatctttg gaagtttccc ttcctaaatt tttattaaca tgatacacat
    18961 caacattttc agttgatcca catatagaac atttaaaatt gttttctagt agggttcttt
    19021 ctttaaataa cttccaatac atagaattga tgtaatcatt ataatttacc ttgaggcatg
    19081 tctcaagaaa gtttatgtat agattgtcta aatctcctgt atttaattct gtatattctg
    19141 gttcaaaata ttcacaacat cctctaaaat cgggctttgt atctgaaata acacatctaa
    19201 tatcatatct atagtctagg ctaggcccct taaatgaaag atgtgtgcaa aaagaacaag
    19261 atgtttctgg tagatttttt tcaagttcat gtaatttatt attcataagt attcctccaa
    19321 aaaatttatt ttaagtatat aaacgaatag attattttaa aacttttatt tatttatatt
    19381 agagatttag gataaatatt ccaataagga gtaaatactt aagtatttat tactgtatta
    19441 aataaaaaag aggcaaatct aacttgagat tgcctacttt tttatcattt tttaataaaa
    19501 tttattctaa gtattatttt atttagattt agtcagccaa cttagtatct gctttgcttt
    19561 ttaactttct accagagcgt acagttaaaa tagcatataa tacagtaaat atggcagcta
    19621 aaacatatga tatatttatg tttaagttaa atccaatttg agcattaaat atataactga
    19681 atataacaaa ggaataaaac ataccaggaa ttaagcttat aatataattc ttttctttta
    19741 ttataaggta aactgtaatc attgcaaatg caaatatggc aatagtttga ttactccaag
    19801 agaagtatct ccacaatata ttaaatccag aagcactaag ctttgcaaat ataaggatgg
    19861 ctatgacagg aataaatata cagattgaca atataacacg atgttttttt tccttttggt
    19921 cataatgaaa gtaatctgca agcattaatc ttaaagatct taaagctgta tctccagaag
    19981 ttattggtaa aacaattaca cctattatag ctatgatacc accaatagaa ccaagtaaat
    20041 ctcttgcaac caaacctatt acatcaggag aacctactaa ttccttaggt attcctttat
    20101 tgtaaatacc catagcagct gcagcccaaa tcattgcaat taaaccttct aaaatcatca
    20161 tattataaaa agtagttttt ccttcttttt catttgaaac tgatcttgct ataagagtag
    20221 cttgagttga gtggaatcca gaaacaatac cacaagcaac tgtaacaaag aatgtaggaa
    20281 taagaggaat accatcagga tgaatacctt tccaatttgc aagacttaaa ttagctaaat
    20341 cataaccttg tgtaaagatt ccaattccaa caccagcagc agataaaagt agaatagctc
    20401 caaatattgg atataccttt cctatgattt tatcaattgg aaatagtgtt gcacacaagt
    20461 aatatagaaa gataagtcca taaactatcc agacaactgg attatttata gtcgatttca
    20521 tatttaaaat ttgagtaaca actaaatccc caggtgtata aataaaaact gcaccaacta
    20581 aaagcattaa aagacaaagg aatatattat agacttgaaa tactttatta cctaaaaatc
    20641 tactgataag agaaggcatt tgtgctcctt tttctcttat actaatcatt ccactcatat
    20701 agtcatgcaa agctcctcca aatacacagc caataggtat tagtatgaat gctattggac
    20761 caaacaatat accttggata ggtcctagaa ttggtcctgt accagctata ttaagcaact
    20821 caattagact atttttccat tttttcattg ggacatagtc tactccatca ctttgagcaa
    20881 gtgcaggtgt ttttctgtca tctgggccaa atactttttc acaataagca ccgtataaag
    20941 cacctcctat tagtaatata gccaagccga ttaagaatgt tgtcataata tcaccccatt
    21001 tataaaattt tttggtcttt gtatacatat tatctaaatg tgtagattta tattgaattt
    21061 aattctggac aacctacaaa ttctataaag tattacaact aatataattt cagaaataaa
    21121 tacatataca taaccatgta cttagtatac aggtatttgg taatattttc aatgttatag
    21181 caataaagaa tatagacaaa attctgataa tattttacaa aaaaattaaa aaatataaaa
    21241 attagtttaa atagatggag aaaaaaggaa aatcaattga gaatataaat gtctatatct
    21301 taacaatatt aagaggttta aaattcttga agaaagaata gtcaagtgat ataatgagtt
    21361 tacaggtgta tatacactag tacatataga aagcggtgca attatggata aagatttaac
    21421 atatcaaatc aaagagctaa agaaagaaaa aaatgcaata atacttgccc atttttatca
    21481 accaccagaa atacaagaat tagcagatgc agttggtgac tcatattatt taagtgagat
    21541 tgctagagat tgtaaagaag aagtggttgt attttgtgga gtaaggttta tgggagaaag
    21601 tgctaagata ttatcacctg aaaaaactgt attgatgcca gtaagtaacg ctgggtgtgc
    21661 tatggcagat atggtagatg aagaaggcgt gataaaatta aaacaacagt atccaaatgc
    21721 tttggttgta tgttatataa actctacagc taaagttaag gcacattgtg atgtttctgt
    21781 tacatcttct agtgcaatta aaatactaga aaacatagat aataaagaaa ttatattcct
    21841 tccagataaa aatttaggag gatatattgc agaacaattt ccagataaaa actttatatt
    21901 ttgggacgga tattgtaaat accataacaa tataagagct gaagaaataa ttgagttaaa
    21961 agataagtat aaaaatgcag aagtattagt acatccagaa tgtaaaaaag aaataagaga
    22021 tttaggagat tatgtaggta gtacatcagg aataataaaa tatgctacta atagtaaaaa
    22081 caaagacttt ataatagcaa ctgaagaagg tatattacat gaattaaaga aaaataatcc
    22141 taataaaaac ttttatattc ctggtggaaa aatactatgt acagatatga aaaagacaac
    22201 attagaaaat ttatatagta cacttaaaaa tatggaaaat gaagttattg tagaagatga
    22261 aatcatggaa aaggctttaa attcactgct taacatgcac aaattagcag aaggatagag
    22321 ggatagactt atgaatttag aacaagatgt tttaatagtt ggaagtggag tttcaggatt
    22381 atactgtgca ttaaatttag ataaaagttt aaatgtgcta gtagtatcga aatcaacaat
    22441 tgaaaacaac aatacatatc ttgcacaagg tggaatttca actgctagaa atattgatga
    22501 tatagaatcc tttgtagaag atacaatgaa ggctgggcag tataaaaata gagttgaagc
    22561 agttcaagta cttgcagatg aatctataga aaatgtaggg cagatagtag aatatggtat
    22621 gcctttagac aaagaaaatg gagagataga ttacacaaga gaaggagcac atagtgtaaa
    22681 tcgaatagtt catagtaagg ataatactgg agaagttgtt tttaaaactt tattaaaaga
    22741 agctaaaact agagaaaata taactcttat agaagatgcc tatcttttag atatattaaa
    22801 agatggcaat aaatgtatag gagctagaat ttttaaatct aaaaaagaga ttcatgtttt
    22861 ttcaaaaata gtagttttag ctacaggtgg aattggtggg ctttttaaaa attctactaa
    22921 tcaaagacat cttacaggtg atggaattgc aatagcttta agaaataata taaaaataga
    22981 aaatttagaa tatattcaaa tacatcctac agccttttat gaagaaaata atgaaggtag
    23041 gagaatgctt atatctgaat cattaagagg agagggagct aaacttttaa ataaaaataa
    23101 agaaagattt gtggatgaac ttcttcctag agatgtagtt tcaaaagcta tttttgagca
    23161 aatggaaaaa gataaattac cttatgtgta tttagatgca acacatttag atagtgaata
    23221 tttaattaat agattttctt ttatttataa tgaatgttta gctagaggaa cagacataac
    23281 gaaagaatgt ataaaggtat ctcctgcaca acattatttt atgggcggga tacatgtaga
    23341 tttggattca aaaacatcta tggaaaattt atatgcagtt ggtgaaatta gttgtacagg
    23401 tgttcatgga gccaatagat tggcaagtaa ttcattactt gaagggcttg tattttcaaa
    23461 aagagcagct aaaaatataa actcagttat agataatgta aaagttaagt ttatagatgt
    23521 accagatatg gatatagata tagaacaggt aaaaaaagaa aataaaatat tagtcataaa
    23581 agaaattgaa agaacaagtg aggattttgg cgatgaatta tttgattatt gatagaatga
    23641 tacaggatgc tttgatagaa gatgttccaa gtgaagatat aacaactaat tctattgtgg
    23701 atgaaaattc taaatctact gtagatttaa tttgtaaaca agatggtata atagctggct
    23761 taggtgtatt taagagagtt tttgagatac ttggtgatgt agatgtaaaa ttatataaaa
    23821 atgatggtga tagagtaaag aatcgtgaaa agatagctgt attaacaggt agtactagaa
    23881 acttacttgt aggagagcgt gtagcattga attatttaca gagaatgagt ggtatagcta
    23941 ctattacaag taggtatgtt gaaaagcttg aaggtacaaa tacaaaatta ttagatagta
    24001 gaaaaacgat acctaatctt agaatattag ataagtattc tgttaaagta ggtggaggat
    24061 gcaatcatag atttaattta tctgatggaa tattgcttaa agacaaccat attggggctg
    24121 ctggtagtgt taagaaggca gttgaattag ctagaaaaaa tacttctttt gttagaaaga
    24181 tagaagtgga agtggaaact ttagacatgg taaaagaagc aatagaagct aatgcagata
    24241 taataatgtt ggataatatg agtttggaaa tggcaaaaca agcagtagat attataaatg
    24301 gaagagccat tgtagaattt tccggaaatg taaatttaga tactatagaa gatataggaa
    24361 aaattggtgt ggatgttgtt tctgtaggag cgcttacaca ttctgttaaa gctctcgata
    24421 taagtatgaa gaatttgaga aatatataaa ttttatattg aaataaactt aattaaaata
    24481 gactccttaa atttgtttta taaatttaag gagtctattt tttattcatt aaaattaata
    24541 aaaatagaaa aaaatcaatc aataatttaa taaaaataga taaatactat atacactttt
    24601 aataatatag ttaatatcta ttttacagat aaataagaat aaacttataa tataattgta
    24661 tctgtaaaat tttgtaaaat ataaaaagta tagggggaat aaaaatgaaa gtagcttata
    24721 tttttagtag tactaataca cataaaattc tagacaaaat gataatacca caacttgaac
    24781 aaggaactca tggagtagac gttctaggga tgtttttctt tatggataat accttatttt
    24841 tatctaaagg taatcctgta ggcgagagac taagtaaatt acatgaaaaa actgatatga
    24901 taataatggc ttgtgaccaa tgtgctatag aaagaggtat agagaataat ttggttgatg
    24961 gagctacaat tggatgtttc ccaaacctat atgcttgttt aggcagtgca ggtgtggacc
    25021 aagttataac gttataaatt agtataggat acaatccata gaaaaattta ttttctgtgg
    25081 attattttaa gtaaaagata aattgtttaa agtaggggat taacaaatgt ctaaaatttt
    25141 agttgaattt ataaacactt gaccatcttg tgatgaacat ggagtgttta taaaaaaaat
    25201 gtcagaaaaa tatgctgata aattggaaat aaaattatat caagcaggga aagatttttc
    25261 gtatataaaa aaatatggga ttataacaaa aggaacttta ataataaatc agaaaaagaa
    25321 atatgataga ttaaataaag atacaattga aagggcaata gtagaagcta taaacaataa
    25381 ttaaaagttt aaaaattagg agtatcaaaa atgaatatat tagaaattgt tttaaatgga
    25441 gttaaagata ctatagtagc ttctatagtt acactgaatg gagcatcagg atggcttatg
    25501 ataagtttta tattggcagg tgtacttcac aatgttttaa ctccaagaag gtttcaaaaa
    25561 caacttggaa ataagaaatt ttcttcttta attaaaagta ctatctcagg aatgttttta
    25621 ccaatatgta gttgtggagt aataccactt ggaataagta tgtattattc aggagcatat
    25681 ttaggaccag tacttgcttt tatgacatca actccaataa taaatccaat agctgtagtt
    25741 ctttgtctag gtcttttagg tcctaaagtt acaatcattt atctaatagc tggtttttta
    25801 gtaccatttt tagtaggatt tttaggaaat tgttttggaa aagaagaatt atcacttcat
    25861 aatgattcag aggaagaagt aatagaatta gaagaagaaa aaagaagttt tatgggaaat
    25921 atgattgatg gtcttaaatg gtcttttggt gaattagccc ttactattag taaatatgta
    25981 gtaatgggta tggtttttgc aggatttata acaactattt ttccaaatag tataattcaa
    26041 aaatatttag gaaatccagg tttgctatct cttggaagta tagcaatact tgcatgtgtt
    26101 atgtatgtat gtgctgtagg acatataccg tttatagcag cccttgttgc aagtggagca
    26161 tctcctggta tagcgataac atttctaata gctggagcag ctacaaatct accagaactt
    26221 ataagtatgt ttaaacttat tggaaaaaga actgttatta tatattctgt aactttgaca
    26281 ctgtcttcaa taataattgg atatataaca aatttacttt tagtgaataa taactcagta
    26341 aatttagatg caacaaattc ttcaatacag ttggcaaata aactaatgtt aaatatacct
    26401 gatagttttg agtatgtatg tactatttta attattttat ttgcatttaa atcaataata
    26461 ccaaagatga aagaggtcat aggaaatcat gcttaaaaag atagttataa tatcctgtat
    26521 agtagttgtg ttaatcattt tatcaaaaat agttgatgat aacatcaaag aagatgcttc
    26581 tattccaaat gtgaataaag aaacattaga atattttaga aaaaattata aagaagacat
    26641 aataacttgt gctgaagaag acttaaataa tgatggaaaa aaagatttag tagttatata
    26701 taaaaaaagc aataattcaa atgaaatggt agtcgtagtt tctgataaaa atagccacta
    26761 tataacaaag ccaataccag cacctataga gaatcaaacg ataaccttta aaaatattga
    26821 tgataaagca ccaatagaag taattgtatc agggtctaaa aatggtaatg taggatatgc
    26881 tatttacaga gtagagggta aaaaatttgt tgatttattt ggcgaagata tggacaaatg
    26941 ttgctaaaaa taaaaataat acataaaggt gggaaattta tatgaaatta tctaagaaga
    27001 ttcaagcatt aatcttattt ggtgtaatgg gtacatctat attaacagga tgtagtagta
    27061 aaccaaaaga aaaagaagaa gcaaaaactg gaaaagatga ttatacaata acagtaggtt
    27121 attataattg tgaccatatg actgcgggac cagtagcaga atcggcagga atatataaag
    27181 atttaggtct taatgtaaaa actgttggaa atggtaaagt tcctgaagct atggcagcag
    27241 gaaaaatgga tgcaggttac ataggaacta aggggttagt tggagcaata cctaagggtt
    27301 cgccaataac aatagcagct aacaaccata ctggtggttc tgaatattta atagtttcaa
    27361 aagatataaa agaacctaaa gatttaatag gtaaaaaaat tgcaacagat atgagtgatt
    27421 tcttatggac aagtgattat ggtccagaaa caggtctacc aactgaccca tcaaaatatg
    27481 aagttgtaaa tatggattca gacaaagata aatatttagc tcttaaaact gggaaaattc
    27541 aagcttttac atcttgtgac ccttggggtt ctgtagctga aaatgacggt gcaggaaaaa
    27601 taatagcatc tactcaatat aaagaaaaag ctaatggcaa agaatataac tgttgttcat
    27661 tctcattaaa caaaaacttt ataaaagaac atccagattt agcaaaaaaa ttggttctag
    27721 cacatacaaa agcaatagaa tatatatata caaatccagc tgaagcagct aaaatatttg
    27781 caaaatatta taatgtagaa gaagaagttg cacttagaac tatctataag aagactgttg
    27841 gagaaggtag aactcttact tggaaagtta caggtgaaga atataaaaat aatttacaaa
    27901 tgtataaaga tttaaaggct ttagatgata tacctaaata tgaagatacg attgatacaa
    27961 gcttattgga ctcttgtgga gctgatgatt ttgacaaatt tataaaagaa aaagttaacc
    28021 cagagtttcc tgaaggtatg agttatgaag actggaaagc aaaggtatta taaataaatg
    28081 gagaatatga aaaatataat aatacttttt atcattattt tgggaagtat atttataata
    28141 ttttttaatt ttgaagataa agaagaaata aaaggaaaat taagaatagg ggtatctgat
    28201 gatacctctg gttttgtaat aaattatatg gtaaatagag actactttga aaatataaaa
    28261 gtaagtgata taatggatat ttttacaatt aatgattgct gagcaagtac aatgcagtgg
    28321 gctctgagtt cagaggaaat agatatagca atcatatgta aagatgctgc aaaacaatat
    28381 gtaaatgtaa atagtggatt tgaaattgtt ggtactgtag ttcaaaattc tgatatattt
    28441 ctattaagtg ataataatcc tgaaaagata ggagtcattc aaaatagaga ttaccagtca
    28501 gaattggtta aaaaatacta caagaatgtg gaagtagctc ccttattagg gactgcacta
    28561 gcatatggat tagaaagtaa tttagtagat ggtgttgtaa tagattgcat aaagtcaatt
    28621 gggttagagg gtaaaagaaa atctacaact aaattaggag attatgatac ctatgtatta
    28681 gttgtaaata aagagtttaa aaaaagtaaa gcatatagtg attttataac actttacaat
    28741 aaaagtgttg atgctttaaa gaataaagct acatttaaga aagtattaaa agagtacagg
    28801 aatataagaa atatagatat ggaggaaata agcaattgga aattaaaatt tctaaagata
    28861 aaagcgatag attaactaag aaaaaaactg atggtagtat ggatatttta tataaaataa
    28921 tactattagt tattatattt ttagtctggc attttgcagc aaaagatata gggagttctt
    28981 tgttacttcc aatgccagta gatgtaataa aaggtttctt tttctgtgtg acagatgcag
    29041 agacagtcac taatcttttt ataacattgc agagagtact taaaggtttt atgtatgcat
    29101 tattgtttgg gcttccaata ggatttatta tgggattttc taaaactttt gaaagggttt
    29161 tatctccagt agtagattct gtaaggcaag ttccaataat ggcatgggtt cctcttacta
    29221 tagtatggtt tggtatagga gatggtccaa caatattttt aatagctttt tcaggggttt
    29281 tccctataat tttaaatact attcaaggag taagagcaat atcaaaggat tattataatg
    29341 cagctagaag tatgggagca tcgccaattg tgatatttac aaatgtaatt gttcccgcat
    29401 ctcttccaga tatactaact ggttcaagga ttgctataag tacaggatgg atgtctgtta
    29461 tctgagccga gtttatagct acgagtgccg gtctcggtta ctcaatggta caagctcaaa
    29521 cattaatgca tacagatgta ttaattgggc ttatgatatt tgcagcttta ataggattta
    29581 taatagatag ggtattaaag tttataaata aaatcctttg tagatggagg tttgcagatt
    29641 aatgaagtta agtgtaagag atataaataa gacatttgta aataatagag tacatacaaa
    29701 ggttttagaa gatataagta tggatataga tgatggacaa tttgtctgtc ttttaggacc
    29761 atcaggttgt ggaaaaacaa ctttacttac tataataggt gggtttcaaa aatcagaacg
    29821 tggagatgtt tttataaatg ataaaagagt taaaaaaccg ggcatagata gagcttttat
    29881 atttcaaaat tatgctttat ttccttggaa aactataaga ggtaatgttc tatttcctat
    29941 gaaacagcag aaaataccaa aagaaaaaag agaagaaatg ctagaagaac ttttggttat
    30001 gtcagacttg aaaggaaaag aaaaactgtt tccgcatcaa ttatctggag gaatgaaaca
    30061 aagagtggcc atgataagag ctttagcatg taatccagag gtattactaa tggatgaacc
    30121 attaggagct gtagattttc aaatgagaca aaatttacag gaagagctag aacgaatatg
    30181 gataaagaag aaaataactg cattaatggt aactcatgat gttgatgaag ctgtatatat
    30241 gagtgataga gttatagtaa tgtcgagaga taaaggaaga attattgatg atataaatat
    30301 agatattcca agacctagaa ttagaggaag ccaaaaatat gaagagtaca aaaacaaatt
    30361 aactgatact ttatcaaagt gttatgaagt ttaaagagga gtagacatga gtcaaataga
    30421 aaaatacaat agtttggata atacacaaca gaaaataaga atctctatta tggatatgat
    30481 aatagataaa ggttctagtg ttacacttca agaagtgact gaatatgtaa gtaaaaagtt
    30541 gaatatagaa aaagaatata tagaaaggac actacaatat tttatttata aaaatataat
    30601 ggttgttgat gggaatagta taaattttat atatcctgta tcagcattgc caacaaatca
    30661 taaagtaact ttgagagata atagaagttt cagtgcaatg tgtgctatag atgctatagg
    30721 aacctcttgc acattcaacc aagatataaa aataaactct atttgtagtg ctacaggtaa
    30781 ggaaatagaa gtaattataa aaaatgaaaa gattgagtat gtgaataatc caaacttaag
    30841 agtgcttcat ataaatcttg ataagcatct aaattgggct gctagttgct gaaatataat
    30901 gaatttcttc tggacgaaga gtgattttga tgcttggaca aacgaagcgg gattatctga
    30961 tgatgaagat atatattgtt tagatattaa tgaggcaata gtagaatcat ataaaatatt
    31021 taaactcaaa caaaaggtac tatcatagat atacatatat gaaaaactaa aaatgacctt
    31081 tagataaaaa tgataggtaa tatccatact atataaatta gtatagagta gggatgtgaa
    31141 aagtatttaa atctaaaggt atatttttag tttttttaga tgattagata gccttattaa
    31201 gtatatattt atttataacc tttgcaactc cattttcatt attagtatca gtaacataat
    31261 ctgcaatttc ctttatacta ttacgtgcat tccccatagc cacaccaaga cctgcatact
    31321 ttatcatatg ttggtcattt tcttcatcgc caacagcaat agcattttct ataggtatat
    31381 ttagattcgt acataatgtt ttaagagcag ttcctttatt agtagtttta cctaaaaatt
    31441 ctagataaaa aggagcagac cttactatag tatatttatt gaataaattt ttaggtagtt
    31501 gctgtatgat ttcttccaat ctttctggtt catcaactat cataatttta cagattttaa
    31561 tagattcatc aattttatct ttttgtatat atttgacttc tatattattt aatttcgctt
    31621 catgaagtgt atatttgcca ggcgtttcat ttggtgtaat taaatttgtc attgtattta
    31681 tgtgtatttt agtcttatat tttttacaaa cgttattata tataaaatca aaatcatcta
    31741 aactcatttc tacatcatgt atgattttct cacttttaac ttcttgaata attgctccat
    31801 taaagcatat aacataatca ccttcatctg ttaaatttag ttgatttaga agttctttta
    31861 caccaggaag aggtctgcct gttgttataa ctacttttgc cccagccctt tttgcctcct
    31921 gaatggactc aaagacttca ggagtaatcc ttttttgtgt atttaatatt gttccatcaa
    31981 tgtctagtgc gattaatttg tacatatcaa cctccaataa attatatact caaatttcct
    32041 gtgaaaacgt tgacacagct agtgttgaac aaagtttcag tgggaaatat ggtttgtttt
    32101 gtatactatt atatcaagag ataagtaatt tagaaagttt aataatatta attttttgat
    32161 aaaaatatta cttatgtgag atgagaatgt aaaaactttg taatcaataa ttccaaaatt
    32221 cttaattaca aataatggta tcgtttgcat tgttaatata aataaatgta tattatatag
    32281 ttagaagttt gaataaatag actgatggta atttgttata ttagttagtt ttgtgttata
    32341 aaatattgta attccattaa tcatatgtta ttattcaaca tgattataat ataaagaata
    32401 tttgatatat ataataattt ataaaagagt attacatagt acatataaat ctatatttat
    32461 aaatacaata aagtaaagtt gtaaaggagt gctttaaatg aagttttcag agtttaagta
    32521 tgaaagaccc aattataata gtatgaaaaa agagtttttg agttgtgtag aagatataaa
    32581 taattctcgt aattataaag agcaacaaaa aaatatacat aagattaatt tattaagaaa
    32641 taaaatagaa acgttgtcaa atatagcttc gataagatat agtacagaca catttaataa
    32701 attctacaaa gaagaaaaaa attattggga tgaatatatg cctttgtatg aagaattaaa
    32761 ttcatatttt tataatgcca ttgtaaattc aaaatttaaa tatgatttga taaaagagtt
    32821 tggagagcaa tttttcacaa tagtagagta ttctttaaaa agtttttcca aagaaataat
    32881 aagtgaattg caggaagaaa ataaattgtg ttcagagtat actagacttt tagcttcagc
    32941 tgagataatg tttgatggga aaataagaaa tttatctggt atggggaaat tcatgtattc
    33001 taagaataga aaaacaagag aattagcaaa caaagcatat tataatttct ttgaagaaaa
    33061 tgaaactaaa tttgatgata tatttgataa attggttaaa cttagagata agatgtctaa
    33121 aaaattaggt tttgaagatt ttgtagagct tgggtacatt agaatgatga gaagtgacta
    33181 cagagaatat atgataaaaa atgttagaaa acaagtacta aaatatgttg ttccaatggc
    33241 aaatgagctt tatgaaaaac aagctaaaag aattggtcta gaatatttaa gttacataga
    33301 tgaaggcgtg gaatttttaa caggaaatgc aagtttaaag ggagattcaa gatacataat
    33361 taaaaatggt aaaaggatgt attctgaact gtcaaaagaa acaaatgaat tttttgattt
    33421 tatgttagaa aatgaactta tggatttaga aacgaagaaa ggaaaaggag ctggagggta
    33481 ttgtacttat ataccagatt ataagtcacc attcattttt tctaatttta atcaaactgc
    33541 tgatgatata gatgtattaa cacatgaggc aggacatgca tttcagttgt atatgtctag
    33601 atggattgat atgccagaaa ttaattttcc aacgcttgat agttgtgaaa tccattcaat
    33661 gagtatggaa tttataacat ggccgtggat ggatttattt tttaaagaag atacagataa
    33721 gtacaaattc actcacttat catcttcaat taaatttata ccttatggag taattgttga
    33781 tgaatttcaa cactatattt acaaaaatcc taatgtagat aagtcaaaac gaaaagaaat
    33841 ctggagattt ttagagaaaa aatacttacc tcatagaaaa tatggtgata attctttttt
    33901 agagagaggt tgttggtggt ttaaacaagg acatatattt aaaaatccgt tttattatat
    33961 agattatgta ttagctcaaa tttgtgcgtt acaattttgg aaaaaaatga ttcaagatag
    34021 agatgcagga tggaaagatt atataaatat ctgtaaagtt ggaggaacaa aatcattttt
    34081 agatattgtg agtatgggaa atttgtattc tccatttgat gatggctgta ttgagtctat
    34141 tataggtgat gttaaatctt ggtttgatga aataaatgac agcaaattat aaaaaattat
    34201 gagaatttta ttgtataaaa gtaaaaaatt aaaaaatata taaaaagtga ttatttgttg
    34261 tgaaaatagt cttgttttta actaaattga taaaataaac taaaaaagat ttgtacttaa
    34321 aaaaaaaaga aaatacaaga aatatgcaat atcaacattt ggaaaaaaaa gtaaaaatgt
    34381 atacaaaata catataaaaa aactattgca aattttatag aatgtttata tacttaagtc
    34441 aacagagata gaaaaaagct atttatagaa aacaaaggat aaattgctta attttaaatt
    34501 ttctgaaaaa aataataatc cggatgaaga gtatgggaga ctgctttata aaaagcgacc
    34561 gaagggacaa gtttaatctt acttacccaa aagattagat gaaattctca ggtaaaggtg
    34621 actgtacttg gacggggctc tgaaaatgat tttaacaggg aagacaaatt gggagatttg
    34681 tttttcctgt tttttattta tcaagtaata aataaaagtt tgaaagagag taaaaaaatg
    34741 ataataatta cgaataatcc aaaagtaaaa gaagaagttc aaggcagaga agttttgttt
    34801 aaagacacaa cttatattgg aatcttagaa gcaagtagag atttgataca tgaaggttat
    34861 gaacttttat ctcatccact ctatgggagt gtaaagccaa atgaaacacc ttacaggact
    34921 gttatactaa agaaaggaaa tcgtttggat ataaactctt taaccttaat tgaagaagca
    34981 atcatcactg caagtaaatt tcagaataat aaaaagactc caaaatggac agaaagtgtt
    35041 caagatgatt ttagagtaat tgattatgat atattttata atacaataca aagaatgcag
    35101 tatgaataaa aaaattgtta aaaagttatt aaaacagcct aaatattaaa tctaatttaa
    35161 tattaaataa aatgagaaat ttaattgggg aggataagtc aaaatggaaa atgtgtatga
    35221 cttagtaata attggttcag gaccagcagg gcttgcagca ggtctttatg gagcaagagc
    35281 aaagttaaaa actctaatcc tagaaaaaga caagactggt ggacaaattg taataacaca
    35341 tgaaattgca aattatccag ggtcagttcc aaatgcaaca ggaccaagtt taatagctag
    35401 aatggttgaa cagtgtaaag agtttggagc agaaatgcta agagacaaca ttgttgatac
    35461 tgagctagat ggagatataa aagttttaaa aggggaaaag gcagaatata gagcaaaagc
    35521 agtaataata ggaacaggag caacaccaag aaagattggt tgtcctggag aaaaagaact
    35581 tactggtaaa ggggtttcat attgtgcaac ttgtgatgca gatttctttg aagattttga
    35641 agtatttgtt gtaggtggag gagacagtgc attagaagaa gcaatgtatt taactaaatt
    35701 tgctagaaaa gtaacaattg tacatagaag acaaggattt agatgtgcta agagtgttga
    35761 agaaaaagca aaagctaatc caaagataga gtttttatta gatacagtaa ttgaagaaat
    35821 caaaggtgat ggaatattag aatcagttgt atttaaaaat aaagttactg gagaaactca
    35881 tgaatacttt gcagatgaag aagatggaac aatgggagta tttgtgttcg ttggattaga
    35941 tgctcaaact gatttattca aaggtaaagt tgatatggat gaaaaaggtt acataataac
    36001 tgatgaagat atgagaacta acataccagg cgtatttgca gcaggagatt gtagatctaa
    36061 gactctaaga caggttgtaa ctgcaactaa tgatggagca attgcatcta tagtagctga
    36121 gaaatacatt gatgaaaaat ttgggaacta gaatctgtaa attaagaaat agtgtctgta
    36181 aatttaatga ataatattta aaattaaaat taatatttaa aataaaaata aaaatttcaa
    36241 gggggaataa gaaaatgtta gatttagata aggctacatt tgaagaagaa gttttaaatg
    36301 cagaaggttt tgtatttgtt gatttttgga gtgaaggttg tgagccttgt aaagctttaa
    36361 tgccagatgt tcataaatta gctgagactt atggggataa aataaaattc tgtaagatgg
    36421 atacaacaaa agctagaaga ttagctataa aacaaaaagt attaggtctt ccaactatgg
    36481 ctatatacaa agatggagaa aaagtagacg aagttactaa ggatgatgct acagttccta
    36541 atatagaaaa catgattaaa aaatatcttt aatatgttga taaattatta acattaaaag
    36601 aaaaaatcta tttttatctg aagataagaa gaaaaaggtt tttatgtcca actttgttaa
    36661 aaaaataatt ttaaataaaa taattttttt acaataaata ttttttagta ataaaataaa
    36721 gatttaaggc ataaaaacac aataatatat ctttaaaaaa ataagataaa ttgtaaaatg
    36781 atataatttt gagaaaaact caaatgcata tacacaaatt aaataacagg aggtgcaagt
    36841 atgcgtcttg aattagggaa aatctttata aaagatattc aatttggtga tgtaacagaa
    36901 gtaaaagatg gagtattatt catcaataag caagagatgc tacaagaaat tggtggagat
    36961 gagcatatta aatctataga tatagaatta gctcgtccag gtgaaagcgt aagaataaca
    37021 cctgtaaaag atgttattga gccaagagta aaagttgaag gtaatggggg aatcttccct
    37081 ggtatcatgt ctaaagttga cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta
    37141 gctgttgtaa ctactggaaa aatagtaggt ttccaagaag gtataataga catgactgga
    37201 gaaggagcta agtacactcc attttctaaa ttaaataatg tagtagtaat agctgaacct
    37261 attgatggtt taaaacaata cgctcatgaa aaagctgtta gaatgatagg attcaaagca
    37321 gcaatgtact taggagaagt agcaagaaat cttactccag atgaagtttc agtatatgaa
    37381 actaaacctc ttcttgaatc aataaaagaa tatccagagc ttccaaaagt aggatatgtt
    37441 tacatgcttc agactcaagg tttattacat gatacttatg tatatggagt agatgcaaaa
    37501 caaatagtac caactttatt atatccaaca gaattaatgg acggagcaat tgtaagtggt
    37561 aactgtgttt ctgcatgtga caaaaaccca agttatgttc atataaacaa tggggttgtt
    37621 gaagacttat atgcaagaca tggtaaagat ataaactttg taggtgtaat tataactaat
    37681 gaaaatgttt atctagcaga taaagaaaga tcttcaaact ggacttctaa attatgtaaa
    37741 tatttaggat tagatgcagt tatagtttca caagaaggtt ttggaaatcc tgatactgac
    37801 cttataatga actgtaagaa gatagaaatg caaggtgtta aaacagttat agttacagat
    37861 gaatatgctg gacgtgatgg aggttctcaa tcacttgcag atgcagatgt aagagctgat
    37921 gctgttgtaa caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt agataaagtt
    37981 attggacatt tagaagtagt agatgtaata gctggtggtt ctgatggaag tttaagagct
    38041 gatggaacta ttgaagttga aatccaagct ataacaggtg caactaatga aacaggattt
    38101 ggtcatttaa ctgctaaagg atattaagaa gtattttatt aaacgattga gtaatgttaa
    38161 taaaacatat tatagataat tataataagt gttatagata tataaaaata tattagaaat
    38221 ataataagga ggattattat gagtttactt agtaataaaa aggttcttat aataggtgac
    38281 cgtgatggta taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag
    38341 gttgttttct catctacaga atgctttgtc tgaacagctg ctggggctat ggacttagaa
    38401 aatcaaaaca gagttaaaga tgctgctgat aaattcggag ctgaaaatgt tgtgatttta
    38461 ctaggtgctg ctgaagccga agctgcaggt cttgcagccg aaacagtaac tgctggagat
    38521 ccaactttcg ctggaccact tgctggagtt gccttaggat taagtgttta ccacgttgtt
    38581 gaggaaccaa taaaatcatt atttgatgaa agtgtatatg aggaccaaat aagtatgatg
    38641 gaaatggttt tagaagttga agaaatagaa gaagaaatgt ctggtataag agaagaattt
    38701 tgtaaatttt aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc
    38761 agtacattat attaatcagt tctttgctgg tataggtgga gaagaaaaag ctgatactaa
    38821 gccacatgtg gcagaaactt tacctccaat aagtttacaa cttgataaat tattaggtga
    38881 agatattgaa atagttggaa cagtagtttg tggagatagc tatttcaatg aaaatataga
    38941 tagtgcaagt gaagaagttt tatcaatggt aaaaggtttt gaaccacaac ttttcatagc
    39001 tggtcctgca tttaatgctg gtagatatgg ggttgctgct ggaacaataa ctaaagttgt
    39061 aaaagatgct ttgaatatac cagctttaac aggtatgtat gttgaaaatc caggagcaga
    39121 tatgttcaaa aaggatgttt atgtagtaga aacttctgat tctgcagcag gtatgagaaa
    39181 ggcacttcct aagatagcta aacttgcagt aaaacttgct aatggagaag aaattggaac
    39241 tcctaaagat gaaggataca tagctagagg aataagagtt aactacttcc atgaagacag
    39301 aggttctaag agagctgttg acatgttagt taagaaaata aaaggtgaac catttgaaac
    39361 tgaataccca atgccaaact ttgatagagt tgacccaagt aaagctgtaa aagatttatc
    39421 aaaatgtaaa atagctttag ttacttctgg tggtatagtt ccaaaaggaa atccagatag
    39481 aatagagtct tcttcagctt ctaaatatgg aacttattct atagcaggag ttatggattt
    39541 aacagaagag acttatgaaa ctgcacatgg tggatatgac ccagtgtatg caaatttaga
    39601 tgcagataga gtattacctg ttgatgttct tagagattta gaaaaagaag gtgtaattgg
    39661 aaaattacat gagactttct atacaactgt aggtaatggt acatctgttg caaactctaa
    39721 aaaatatgct tctgaaattg gagcagcatt agtggctgat ggtgtagatg cagtaatatt
    39781 aacttctaca tgaggtactt gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa
    39841 aactggactt ccagtagttc atatgtgtac agtagtacct atttcattaa cagtaggggc
    39901 taatagaata gttccaacta tagcaatacc tcatccactt ggaaatccag cgcttgaccc
    39961 tacagaagaa aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg ctttaactac
    40021 agaagtagat ggtcaaacag tatttgaaaa ataaaaataa cacacacata tattatatag
    40081 ataaaaatat aaacacacac taaaaataaa aaataactat acacaaaaaa ataatataga
    40141 tttcatgaat ggataaattg gagttattca ttcatgaaat cttaaaaaat gccaaaatac
    40201 ataaacaata atagaatcaa atatacaata aaatacaatg attagagctt ttaataagtt
    40261 taataagatt aaaaaataag tgttcataaa atttatggag gtgcacaaaa tgacttatcc
    40321 agttttaaaa ggggcaggct atgtacttat tcatactcca gatatgatag tgcaaaatgg
    40381 aagtacttgt acagttgaaa gagcaacaaa tccagattca gaatttttaa aagaagtaag
    40441 taatcatata agaagttatg aagatgtagt taactacatg ccaaatcaag tttatatagg
    40501 aaatagaaga ccagaagaat taagagattt accaatgcca tggtgtgaac aaaagattga
    40561 gggaactaga aatggtaagt ttggagaaat aatgcctcaa gatgagttca tagctttaat
    40621 gcaaataagt gatgcatttg atttagtaaa attatcacaa gaatttattg atgaagtaaa
    40681 gccaaaaatc gaaaataact accctgaaat tgcaccattt gtaggtaaat taaagggtga
    40741 tgatatagaa gaaggtaaag aattagtagc aacacacata gctgaaggat tataccatga
    40801 tggaaaattt gttggttatg taaaaagagc acatgatgta gatgttaact taaatgccca
    40861 tacaatgttt gaaaatctag tagtaaaagc atctggagtt ttatctgcta ttcaaatgct
    40921 aagacatagc aaaatagacc ctgctgaaat agattatgtt attgagtgtt cagaagaagc
    40981 ttgtggagat ataaaccaaa gaggtggagg aaactttgct aaatcaatag ctgagatagc
    41041 aggacttcaa aatgcaactg gttctgacac aagaggattt tgtgcagcac caactcatgc
    41101 attaattcaa gcagcagctt tagtaaaagc tggtatacat aaaaatgtaa tggtagtagc
    41161 aggtggagca agtgctaaac ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc
    41221 tgtacttgaa gatgttgttg gaggatttgc agtacttgta tctgaaaatg atggtgtaaa
    41281 tccagttata agaactgatt taacaggtaa gcatactgtt ggaactggtt cttcaccaca
    41341 agcagttatg acagcactta taacttcagg tcttgataga gctaatttaa aaataacaga
    41401 tgttgatgtg tattcagttg aaatgcaaaa cccagatata actaagccag caggagcagg
    41461 agatgttcca gaagcaaact acaaaatgat aggggcactt gctgttaaac gtggagactt
    41521 agaaaagaaa gaattgaaag attttgtttc taacaaagga ttaccaggat gggcaccaac
    41581 tcaaggacat ataccatcag gagctcctta tataggattt ttaatagatg atttaactac
    41641 aggaaataga aatagagcta tgatagttgg aaaaggtagt ttattcttag gaagaatgac
    41701 taacttattt gatggagtat cttttatagc tgaaagaaat acaggtgtta ctgaagagac
    41761 ttcaggaata tctaaggatg aaattaagaa gataatagct gaatctatga aaaaactagc
    41821 attagatatg ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat
    41881 tcttagaagt agctaatgca atagaaagcg gcgaatttgg caaaaaagta aaaattggtg
    41941 ttactacact tggaagtgaa catggtgtag aaaacatggt taatggtgca caattagcaa
    42001 aatctaattt atttgatatt gtgcttattg gaccaaaagt ggaaactgac cttgaagtag
    42061 ttgaagttaa tgatgaaaaa gaaatgcatg caaaaatgga agaattatta gattcaggat
    42121 atattgatgc ttgtgttact atgcattata attttccaat aggtgtatct acagtaggta
    42181 gagtaataac tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg
    42241 ctactaatag aatagaggca atggtaagaa atgccattta tggtattgct acagctaaat
    42301 caatgggtaa caaatgccca aaagttggaa tacttaatgt tgatggagca agacaagttg
    42361 agaagtgctt aaaagaatta aaagataatg gatatgacat ggagtttgcg gattcaatta
    42421 gagcagatgg tggatgtgta atgagaggta atgatttact tgtaggtgca cctgatgtta
    42481 tggttactga tacattatct ggaaatatat ttatgaaagt attttcttca tatactacag
    42541 gtggagatta tgaagctcaa ggctttggat acggacctgg agttggagag gactatgata
    42601 gaaaggtact tatagtatca agagcatcag gttctccggt agtagcaaat gctttaaaat
    42661 acgcttatga tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg
    42721 ttaaaaaagc aaaatttgat gacattatat cctctcttac taaaaaagaa gttaaagctg
    42781 aaaaagtaga agttaaaatg ccagataaag aaattgttac tcgtcaaata gctggtgttg
    42841 atataatgga tttagaagat gcagtatctg agttatggaa aaacggaatc tatgctgaaa
    42901 gtggtatggg ttgtacaggt ccaatagttt tagtaaatga agctaaagga gacttagcag
    42961 ttgaaacttt agttaaagct ggatatactg caaaataagt acatttttta tatgtaaata
    43021 agaacatata gttataattt tattgattat aattatatgt tctttttatt ttatatcttg
    43081 tcaatattta tatattaaca caatccattt atttgattct ctagctaatt tattatattt
    43141 attcagttaa aattaagctt tctaactaaa taaaaactaa tgaagtatta aaatgaaatt
    43201 gataaaatat gaaaaaattt taggaataaa gtatctgtta gataaaaata agattaatta
    43261 gtgatataat ataattaaga taaaatttat tatttggagg caaaatcatg agcagagtaa
    43321 aaaatgtagt agaattgttg gaaactaaag gtgttgatgc actatatcta acaaagaaaa
    43381 ctaatgtaaa ctatataagt ggttttcctg atgaagaagc atatgcagtg atttgcaaag
    43441 atggaaactt tttagttact gatagtagat atatggagct tgcagaaaag gtttgcaaag
    43501 attttgaaat tataaattgg cataattttg atagaagtgt ggcaaaggca gttaaaagtg
    43561 tatgtgataa agtgggtata aagaaacttg ggtttgaaag aactaatata gtgtttgata
    43621 aatatgaaga acttaaaaat ctgattgaga aggataatgg agaattaata cctacagaaa
    43681 atattgtaga gactttgaga tatgtaaaag acaaagatga aatcaaaaat acaagaaaag
    43741 cttgtgaaat agcggataag gcattagaag aattgattcc acatataaaa gctggagtta
    43801 gcgaaattga actggctaca aagttagagt actttatgaa aatgaacgga gcacaaaata
    43861 tagggtttga aactatactg atatcaggag caaagacttc attgcttcat ggtaagccta
    43921 gtgataagat tatagaaaaa ggtgattttg tacttataga ctatggcgct atgtataatg
    43981 gctatatatc tgatacaact agaactttta tagttggagg agcttctgaa aagcaattag
    44041 aaatatataa tttagttaag gaagctcaaa atgtaggggt tgagaacatg aaagctggtg
    44101 tacatgcgac aattccagat gctgagataa gaaaagttgt caaaaaatat gaagactatt
    44161 actatcaagg tataggacat ggagtcggta gagatgttca tgaagaacct ttcattggta
    44221 attatggtga taaaatcata gaagagggat gtataataac aatggaacca ggtatttatt
    44281 tccctggatg gggtggagtt agaatagaag atactgtttt gattactaaa aatggtccag
    44341 aaagacttac taaattccct aaagatttga tgattttaga caaataattt aatttatata
    44401 tattttttta cataaaatat aacataatct cactattaag ctctatttac atattatagt
    44461 aaagagggct taattttatt gtgagaggtg atagtatata tgtataaaaa acataaattt
    44521 ataaaaaata taaaaaaagc ctggtataaa atcaaattaa ttgatagatg ccttataatt
    44581 gtaatggcaa tattaatgtt tcaatctata tataatttat ttgttaatga agtaaattca
    44641 caagatacta caactattga tgtagtagta agaaccactt cagcagctat atttgggtat
    44701 tttttaagtg ctaattttat aaagagacct ttaaggaagt atacaaatac tgatgtatct
    44761 tcaaatcttt ttatagataa taatttatcc ccaaaaggtc aagagtctaa tcaaaataat
    44821 ataatgaatg tcaaaaatac cattggattt acttcagaag ctgataatta tgaaaagaaa
    44881 atacctatta ataataatga aggttttgaa gaaggagaaa ctagtgaatt gcaaattatt
    44941 atagctactg taatttgtat agtagcatta acagtacttt ttgttgtaag aaattttaca
    45001 actacaacat cagcttcttt aggtactatt tcacagatga gagattttgt ctcaggatgc
    45061 gtgggttttt tgctaggttg tccaagtaaa cctgattaaa aaagtattat caataaaagg
    45121 tattttaaaa agtatcagta taataatatt tagatttaaa tgtatacaat tataattcta
    45181 aacattctat attgtatttt tatactgata ctctaaacta tactatttat taaaatattt
    45241 taacattgta tttaaacaaa atattatatc tgtataatat aaatttagtt tatatgaata
    45301 gatgccatat actcaataaa aagtttcata ctatgagata tgtattgatt tcttctccaa
    45361 agaaccccaa tattcgcagt aattgcacgt tctaatggta ttgaacagag attttctgta
    45421 ttttgaaata tttctttata caatattgtt gaagccaagt cattttctag taaataacgt
    45481 atggtagaaa gttgatttga gtgtaaaatt atatttggct tagctttttc aagtcttttt
    45541 gtaataatct tatagtgata agaaccttca gataagatta caaaaggtac attttttaac
    45601 atataattcg atataatggg atgttttgcc aattcatttt tgggatttgt aactaagtat
    45661 aattctgttt tatagattgt ctttgaatct atatcatcat aaatatcact gtcaattata
    45721 ccaatacaaa aatcaagtgt agattcttca atcattttag ctccaacaaa tgttggaact
    45781 tcaaaaattt taagctctat atctggataa attacattaa atctaggaag tatttttgaa
    45841 agcaaaaacg tacctaaaac tgtaggaata cctattttga gagaagcttt tctctttttt
    45901 cctaaatcaa gtgcgtcaca ttgaaaatcc tcaaattgtt ttataaaatg ttttgacttg
    45961 tctaaaaaaa tttttccttc aggcgttaac tctattttat tacctattct gtaaaaaagt
    46021 tcaaagccaa gttctttttc taattcttta attgctaatg ttatggatgg ttgagaaata
    46081 tgaatacttt ccgcagcctt agtcatgttt ttatatttac agacagtttg aaaatagtat
    46141 aaatagttta tattcatttg gtattagttc ctttcattta catattagtg acaagtatat
    46201 aattaacaat agcataagtt ttgtatattg ttgtaaaaaa atagaatagt tttaaactat
    46261 catagcataa gtttttgata ttttcaatct attaagaata gtgctattat taaattaaca
    46321 aaagagaata gctggtatgg tttagagtaa atcaatctat attacagtta tatcaacgaa
    46381 tatcaaaaaa ttatctatta gaaaataaaa attattttta caattaaatt gttaattaaa
    46441 tcataaagaa aaaagtgata ttaacaagat aaaatttttt taatcagaaa tgttaattta
    46501 ataacaaaat agagtattgg ggagaaagga gaaatatatt ttttaataat atttgttaat
    46561 ttaataacga aaaatgaaag ggagtgattt agtgaagttt gaattgatgg attttttaat
    46621 gaatcctttt gtccttatgt ttgctgcagt aattacagga attttatttg gaaaaattaa
    46681 atttgggaag tttaattttg gggtatcagg agcattattt acaggattat ttattggatg
    46741 gcttgcatat agcttgggga atctaataat agcaaaaggg gaaacagcag caggatataa
    46801 ggcagcaact gtaatgatgg gtaatggaat tatttcttct gacttctttg atttcttttt
    46861 aataatattt gttgcagcag ttggtctatt ggcagcaaaa gatatgaaag ctgtattaaa
    46921 gaagtatgga gcaagatttg taattttagg agttcttata acttttatag gtggctctat
    46981 gacttatgca atgactttac tatcaagtga taaaggaagt agtgcttatg aagtttctgg
    47041 cgtttacaca ggagcattaa caagttcgcc tggattagct gctgctttag aaacagctgg
    47101 taaacatgca gaagatgtat caaaagaatt tgaaaaagct tcaataaaag ataaaaaaga
    47161 aattttaaaa gtagtcgacc cagaaggcaa attggatgtg aatacaacta catcactgac
    47221 tcaagagcaa atagataagt atattgcata tgctgaagct ggtgttggta taggtcatgc
    47281 agtcgcatat ccttttggag ttttaatagt aatcttaggt gtaaatttcc ttccaaaatt
    47341 atttaggatg gacctaaaag aagaaagaag aaaatatgag aaggaaatga aggaagcaag
    47401 agatagtgtt tctggtaaaa cgataccaga agttccattt aatataataa catttttctt
    47461 gacatgttta gctggatact tagttggagg aatacatgta tttatgggac cactagggta
    47521 cttcacacta ggcgcaacag gaggttcact aattgtatct ctagttcttg gatatatagg
    47581 taagataggg gtagtaaatt tcagaatgga agaaaaggtt ttaaatattt taaaacaaat
    47641 agggctagta tttttcttag caatagttgg attaagatat ggtggaaaag tagttgattc
    47701 aattatgaca tctggtatgc acctagcctt agtagcaata gcagtaggtg ttacagctat
    47761 gatgatagga ttcttagttg gcaaatacgt atttaaatta aattggattt tattatctgg
    47821 agctgtctgt ggtggtatga catcaacacc aggtcttgga gctgctgttg atgctcttga
    47881 tagtgatgac ccagcagcag gttatggtgc tacgtatcca tttgcattat taactaaagt
    47941 tatattggtt atagttttac acaaacttcc aatgtaaata agattttata tggggggata
    48001 tttatgaata atgaaggagc atttgaagaa ttaagtggaa gattaagaaa taaagaattg
    48061 tgtgatttaa taatgaatgc agatgaagct gctaaaatta tagaggatgg catggttatt
    48121 ggagttagtg gatttacacc ttcaggctat ccaaaagcag taccattagc agtttcagaa
    48181 agagctaaaa gtggagaaaa gataaaatta accgtatatt caggagcatc tttaggtcca
    48241 gaagttgatg gagcatggtc agaagcaggt ataattgaaa gaagacttcc ttatcaaact
    48301 aattcaatac ttagaaataa tattaataaa ggtgttgttg attatataga tatgcaccta
    48361 agtcattcta ctcaattttt aaattatgga actataccta aagtagatgt agctatagta
    48421 gaagcacttg caataactga agaaggaaat attataccaa ctagtgggat aggtaattct
    48481 ccatctttta taaaaagtgc agataaagta atagtagaga taaatcttgc aaaaccaatg
    48541 gaaatggaag gtatggcaga tatatatatt acggaaaatc caccaaatag aaaaccaata
    48601 gaaataaatc atcctaagga tagaattgga acaacatata taccttgtgg tttggataaa
    48661 attgcaggag tagtaattac caatatgcaa gataagaccc gtccattggg agttgtagat
    48721 gaagcttcga aaaaaatatc aaataatatt attgcttttt taagagaaga agtaaaggca
    48781 ggcagattat caaagaattt acttcctctt caatctggtg taggaagtgt agcaaatgca
    48841 gtattatatg gattatgtga atctgaattt gaaaatttaa cttgttatac agaggttgta
    48901 caggactcta tgttggattt aataagaatg ggtaaagtta caatggcatc tactacatct
    48961 gtaagtccat ctccagaagg attaataaaa tttgaaaaag atattgattt ttttaaggat
    49021 aagattatat taagacctca agaaattagt aacaatccag aaatagccag aagaattggt
    49081 gtaattgcaa tgaacactgc aattgaagtt gatatttatg gaaatgtaaa ttctactcac
    49141 ataatggggt caaagatgat gaatggaatt ggtggttctg gtgattttgc tagaaatgga
    49201 gcaataacta tatttagtac tgagtcaatt gcaaaaaatg gtgatatatc atcaatagta
    49261 cctatggtat ctcacgtaga ccacactgaa catgatgtaa tggtaattgt aacagaacaa
    49321 ggatatgctg atttaagagg tcttgcacct agagaaagag ctataaaaat aatagaaaat
    49381 tgtgcccatc cagattataa agaacaactt agggattatt taaatagagc ttgtaaaagt
    49441 ggagcaaaac aaacacctca catattggac gaagctttat catggcatag taaatttatg
    49501 agtacaggta caatgaaaaa ggctgaaact tttaaaagtg cattataaaa tatttaataa
    49561 attggaggta gattttaaat ggaaaaagca gtggaaaact ttgaagattt aagtaaggaa
    49621 tacattaatg gttatattga gagagcaaga aaagctcaaa gagagtttga atgttatact
    49681 caagaacaag tagataaaat agtaaaaata gttggaaaag tagtgtatta taatgctgaa
    49741 tatttagcta aacttgctgt agaagagact ggaatgggag tatatgaaga taaggtagct
    49801 aaaaataaaa gcaaagcaaa agttatatat aacaatttaa aagataaaaa atcagttggt
    49861 ataattgata tagatagaga aactggaata acaaaggtag ctaaaccagt tggagtagta
    49921 gcggcaataa ctccttgtac aaatccaatc gttacaccta tgagcaatgc aatgtttgca
    49981 cttaagggaa gaaatgcaat tattatcaca cctcatcata aagcaattgg atgtagtaca
    50041 aaaactgtag agatgattaa tgaagaatta gaaaaaatag gagctccaga aaatttaata
    50101 caaatacttg accaacaatc aagagaaaat acaagaaatt taatttcatc agcagatgta
    50161 gtaattgcca ctggaggaat gggaatggta aaagctgcgt actcaagtgg aaaaccagca
    50221 cttggtgtag gggcaggaaa tgttcaatgt atcatagata gagatgtaga cattaaagaa
    50281 gcagtaccta aaatcatagc aggaagaatt tttgataatg gtatcatttg ttcaggtgaa
    50341 cagtcagtaa tagtagcaga agaaatgttt gataaaataa tggatgaatt taaaaataat
    50401 aaaggtttta tagttagaga taaagtacaa aaagaagctt tcagaaatgc tatgtttgta
    50461 aataaatcta tgaataaaga tgcagtaggt caatcagttc atacaattgc aaaaattgca
    50521 ggtgttgaga taccagaaga tacaaaaata atagtaatag aagctgatgg accaggagaa
    50581 gaggatatta ttgcaaaaga aaaaatgtgt ccagttatat ctgcatacaa gtataaaagt
    50641 tttgaagaag gtgtagctat tgcaaaagca aatttaaatg tagaaggaaa aggacatagt
    50701 gtatctatac attcaaatac agttaaaaat atagaatatg caggagaaaa tatagaagta
    50761 tcaagatttg ttattaatca atgttgtgct actagtgctg gaggaagttt ctttaatggt
    50821 cttgcaccta ctaatactct aggttgtggt tcttggggaa ataacagcat ctctgaaaat
    50881 cttgattata agcatctaat taatatttct agaattgctt attatatgcc agaaaatgaa
    50941 gtccctacag atgaagaact ttggggataa aatttagaca aaaaatattc attctaaatt
    51001 aaagtaggaa tatattttaa tttagaatga atttaagaca taaattaaat ttaaattagt
    51061 ttattggact aaatttaaaa tattaagaga catatagaag aaggaagtat tataatttaa
    51121 aataaataaa aaaatctata aaagagaagg agtggtttta ttatggcatt aatgacagga
    51181 gctcaatata ttgaaagttt gagaaaatta aatactaagg tttatatgtt tggtgaagag
    51241 gttaagaatt gggttgacca tcctatgatt agaccatcta taaattgtgt agcagcaact
    51301 tatgacttag ctcatgaccc agaatatgca gatttaatga cagttacttc aaatatcaca
    51361 ggtgaaaaaa taaatagatt tggtcatctt catcaaagtg ttgatgattt gataaaaaaa
    51421 gtaaaaatgc aaagactatg tggtcaaaaa acagcttctt gtttccaaag atgtgttggt
    51481 atggatgcgt ttaatgcagt gtattcaaca acatttgagt gtgataaagc ccatggaact
    51541 aattatcatg ataattttgt aaaatactta acttatatac aagaaaacga tttagtagtt
    51601 gatggagcta tgactgaccc taagggagat agaagtctat cacctagtgc tcaacctgac
    51661 ccagatatgt tccttcatat agtagaaaga agagaagatg ggataattgt aagaggagca
    51721 aaggcacatc aaactggttc aataaattca catgagcacc ttataatgcc tactataagt
    51781 atgactgaag cagataaaga ctacgctgta tcatttgcag ttccttcaga tgctgaagga
    51841 gtgtttatga tttatggaag acaatcttgt gacactagaa aattagaaga aggtgcagat
    51901 gttgatttag gaaataagga gtttggaggt caagaagctt tagtagtatt tgataatgta
    51961 tttgttccaa atgatagaat tttcttatgt ggagaatggg atttctctgg tatgttagta
    52021 gagagatttg caggatacca tagacaaagt tatggtggat gtaaagtagg agtaggtgat
    52081 gtaataatag gagcagcagc tttagcagct gattataatg gagcaaacaa agcttcacat
    52141 ataaaagata aactaataga aatgactcac cttaatgaaa gcttatattg ctgtggtata
    52201 gcatgttcat cagaaggtca caaaacagaa gctggaaact atcaaataga tttacttctt
    52261 gcaaatgtgt gtaaacaaaa tgtaactaga tttccttatg aaatagtaag acttgcagaa
    52321 gatatagctg gagggcttat ggttactatg ccttcagaaa aagactttaa atcagacctt
    52381 aaagttggaa caagtggaat gactattggc gaagtatgta acaagtattt taaagcttct
    52441 tcagtggctt caacagaaga aagaatgaga atattaagat tcttagaaaa tatatgcctt
    52501 ggttcatctg ctgttggtta tagaactgag tcaatgcatg gggcaggttc accacaagct
    52561 caaagaataa tgatttcaag acaaggaaac ataaatcaaa agaaagaatt agctaagaaa
    52621 attgcgggaa taaaaaaaga ggaagcttta aatttataaa aagttgatat ataaagggaa
    52681 attatatatt aatgtgtgtt aattaatgaa aaaatatgta gtataagaga tagttataaa
    52741 attatggtgg tttttagcta agtaaagaaa tttttaataa ggagaattta cacatgacct
    52801 gtggagttag attttgtggg ggctgtaacc ctagatacga aagagggaag gctcttacaa
    52861 taatcaaaga gcacttcaat ggtagggtag attttagtat agcacaagag aacgtcgagt
    52921 atgatttgtt acttataatt ggaggatgcg caactctatg cgcatcctat caacaatata
    52981 aagtgaaaaa tgaatgtgtt aagatgtggg attttagcca tatagaaaga gttataaatg
    53041 tgattgaatc agcaatttgt gaaacttaaa aaagctaaat ggggggattt cattgagttg
    53101 gcaagaattg tatcaaagta aattatgtag tgctacagaa gctgtaaaac agattaaaaa
    53161 cggtgatact gtagtatttg cacattgtgt aggtgaacct cctgcactag tagaagctat
    53221 gattgaaaat gctgaacaat ataaagatgt tgagattaaa catatggtaa gcttgggtag
    53281 tggtggatat actgctaagg gaatggaagc tcattttaga gtaaatccaa tgtttgtaag
    53341 tggaaatgta agaaaggcaa ttgaaaatgg agatggtgat tttacacctg cattcttcca
    53401 tgaagtacca aagttgctta gagaaaaaag attaaaatgt gatgttgttt tagcacaggt
    53461 aactccacca gatgaacatg ggtattgtag cttaggaaca tcagttgatt atacttatga
    53521 agccattaaa actgctagaa cagtaattgt tcaagtgaat gaccagtttc ctagaaccta
    53581 tggtgaggta gtccatgtat cagagtttga ctatatagtg gaaaaatcac aacctttatt
    53641 tgaactacaa cctgcaaaga ttggagaagt agaagaagca attggtaaaa attgtgcttc
    53701 attaattgaa gatggctcta ctttacagct agggattggt gggataccag atgcagttat
    53761 gttattttta actgataaaa aggatttagg gattcattca gaaatgattt cagatgggac
    53821 tttggccctt tatgaaaaag gtgttataaa tggtaaatat aaaaattttg ataaagaaaa
    53881 aatgacagtt accttcttaa tgggtactaa aaaattatat gactttgcaa ataataaccc
    53941 tgcagtagag gtaaaaccag tagactatgt gaatcatcca gcaattataa tgaaacaaca
    54001 taagatggtt tctataaatt ctgctataca agttgattta atgggacagg tagttgcaga
    54061 ggctatggga cttagacaat ttagtggtgt tggaggtcaa gttgacttta tacgtggagt
    54121 gtctatgggt gaagatggaa aggcaattat agctatgcct tcaataacta caaaaaaaga
    54181 tggtacagta atttcaaaaa tcgtatctat tgtggatgaa ggtgcaccta taacgacatc
    54241 aagaaatgat gttgactata ttgtaacaga atacggtata gcagaattaa aaggaaaatc
    54301 tcttagagaa agagcaagaa atttaataaa tatagctcat ccaagtgtaa gagaacatct
    54361 agcagtagaa tttgaaaaga gatttaagga gaaatattaa taatagaggg gtgttaataa
    54421 tggaagcact tagagtagtt ccaaagatat tttactttga tacatttaaa gaatttaatg
    54481 aagaatttaa aataggaaaa aatgatttag taattactaa tgagtttata tatgagccat
    54541 atatgaaacc tcttggaata gatacaaatt taatttttca agaaaagttt ggaacaggtg
    54601 aaccatctga tgaaatgata gactccatga caaaggaaat gaagaaatac aattttgata
    54661 gaattattgc atttggagga ggaacaatag ttgatatatg taagatatta gcacttgatg
    54721 ttccagaaaa gtctattgat ttatttgaag gagaagtatc acctaaaaaa gtaaaagagt
    54781 tagtagtagt tcctacaaca tgtggtacag gtagtgaagt aacaaatgta gctattgcag
    54841 agttaaaatc taaacatact aaaaaggggc ttgcagtaga agaaacatat gcagactatg
    54901 cagtgttaat tccagagaca gtaaaagggt taccttacaa attctttgtt acaagttcgg
    54961 tagatgctct tatacatgct attgagtctt atctttctcc aaaagcaagt ccatttacag
    55021 agatgtattc tctacaagca ataaaaatga ttatggatgg gtataagaag atagttgata
    55081 aaggagaaga agagagattt aatcatctta gagactttgt acttgcatca aattatgcag
    55141 gaattgcttt tggaaatgct ggttgtgcag cagttcatgc actatcttat tcaataggtg
    55201 gagctttcca tgttgcacat ggtgaagcaa attatcagtt ctttactgaa gtatttaaga
    55261 tgtattctag aaaaaaacct aatggaaaaa ttataaaatg tacaaaaatt ttagcagatg
    55321 cccttgaatg tgaccctaac tgtgatgttt atggagaatt agaaaagttt ttaaataaat
    55381 taatagctaa aaaagctttg agagaatatg gaatggtgga aagtcagata gatgaattta
    55441 cagatagtac aatagcaaat caacaacgac tacttgctaa taattatgta gagcttagta
    55501 gagaggaaat aagagagata tttgcaaatc tttattaata caatttttta tttatatgta
    55561 tagtcaacat aaacaatgct aattatattt atgtacaaat aaaaataagg gaacttaaat
    55621 tttaattaaa gttaagttct cttattttta tatggagtta aatataagta tatttttaat
    55681 ttaaaatatg cttatattta agtctattac tttatttgaa acaatttgta gaatttcaaa
    55741 ttccttgaca gtaataagta agtaattgta taatcaaact gcaatatatt aataatatta
    55801 gtgaaaatga aaggattata aaatatgtat aaatcagaaa atttttcaaa aaaatctgta
    55861 ttaataaatt catttttaag taaattttta aaaataattg gatggggttt ggtaagctta
    55921 tttacattat cagccttagt actttcaact atttccatta taattggatg ggctgtaata
    55981 cttcctattc aagtatgtgc agtattttta cttataggag gagtttgtat ttttaaaggt
    56041 aagagattag gagaccaaat ttctcgttat aaaaaatatt gtacaattat aaacaataga
    56101 aatattattc cagtagaact tattgcagaa aacacatcta aatcattaaa ttttataata
    56161 aaagatgtac aaaagatgat agacaaaaac tactttataa atacttatat tgataaaaga
    56221 aataatcaga ttgtgttaac aaatgaagat tttgttccac caagatatga agatgtagca
    56281 tatgaagtca aagaagaaga aaagactgat gaatccaatg aagttgatac aattataaat
    56341 aaaggaatga attatctaac acaaataaaa gaagctaata aaaatattaa atcagaatca
    56401 atgggcgata aaattgtaca agtagaagat gttacaagta aaatatttga tgttgtaaaa
    56461 catgacccat caaaacttac tcagatacaa aaatttatgg attattatct tccaaccact
    56521 ttaaaactat taaattcata tcatacattg gaagaacaag gaattgatag agaaaatata
    56581 acaacaacta tggaaagtat tgaaaataca atgaatacga ttgtagtagc ttttgaaaat
    56641 caattggact atctgtttga agatgaagcc attgatattt ctacagatat tactgtttta
    56701 gagaatatgt tagtccaaga aggattaacg agtggtaaat tttaacttca atagaaaaaa
    56761 tattctgtta tttttaaaca attaattagt cataaagtat aaaatgtgta ttatataagt
    56821 taatcagtac tttatgaagg aagaggagag aaagatacaa tgaataattt agatgatata
    56881 ccagttatgc caactttgac acttgaccca tttggagaag aatcaaacac taatgatata
    56941 gataattctg acttattaat gaaaaaggat gaaaaagacc cagaagaaga aaaactgtct
    57001 gaatctgaac gcaagatggt aaaggaattt gcggaaaaaa tagatattac aaatacaaat
    57061 atgattttgc aatatggagc tggagctcaa aaaaaggtag caagtttttc tgaaacagct
    57121 ttaaagagtg taaaaacaag agatttagga gaagttggag atatgcttac aaatctagtt
    57181 actgatttaa aagcattttc agcagatgaa aaagaacaaa gtggtttttt aggaattttt
    57241 aaaaaggcaa ataataaaat ttcaaattta aaaactaagt atgatagtgc agaggtaaat
    57301 gtggataaag taagcaaaga gcttcaaaag catcaggtta aaatattgaa agatatagca
    57361 atgcttgata agatgtatga attgaactta gcttactcaa aagagcttac tatgtatatt
    57421 atagcaggaa aacaaaaatt aaaggatatg aaagaatatg aaatgccaaa gttgagagaa
    57481 aaagctcgtt tatctggttc aacagaagat gctcaatctg taaatgatat ggtttcactt
    57541 tgtgacagat ttgaaaagaa gattcatgac ttagaattaa ctagaatggt atcgcttcaa
    57601 atggctcctc agataagatt ggttcaaaat agtaataatc tgatggcaga aaagattcaa
    57661 tcaactatag taaataccat tccattatgg aaaaatcaaa tagtgcttgc tcttggaatt
    57721 tctcacttaa atcaagctat gaaagcacag cgtgaagtta gtaatatgac aaatgagctt
    57781 ttattaaaga atgcagaaac tttgaagatg ggaacaatag aaacagcaaa agaatcagaa
    57841 agaggaattg tagatataga cacaattaag aaaactaatc aatctctaat atctactatt
    57901 gatgaggtag ttaaaataca acatgatggt agaataaaga gacaagaagc agaagttgaa
    57961 ttatcaaaaa tagaaaatga acttaaaagt aagcttcttg aatttacagt taaataaaaa
    58021 ttatactaat ataaaaagag actgtctcaa aatagagata agtctctttt tcattgttta
    58081 aatgattaaa gtatatttat attataaatt tctattatct tttaacataa catcatgagc
    58141 tccaccttca actatactag tagaagatac aagtgtaagt ttagcatttt tttgaagttc
    58201 aggaatgcta agtgctccac agttacacat agtagaacga actttactta aagaaagagt
    58261 tacattgtct ttcaaagaac cagcataagg cacataagag tcaacacctt cttcaaaaga
    58321 aagtttctta tctccaccaa ggtcatatct ttgccagttt ctagcacgag cagaaccttc
    58381 tccccaatac tctttcatat aactaccatt tatattaact ttgttagttg gactctcatc
    58441 aaaacgagag aagtatctac caagcattat aaaatctgca cccatagcta aagctaaagt
    58501 tatatgatgg tcataaacga taccaccatc agagcaaatt ggaatataaa tacctgtttt
    58561 ttcaaagtat tcgtctctag cttgagcaac ttcaattata gaagtagctt gtccacgtcc
    58621 tatacctttt tgttcacgag ttatacatat agaaccacca ccaataccaa ctttaacgaa
    58681 gtcagcacca gcttcagcta ggtaaaggaa tccttcgcgg tcaactacat ttccagctcc
    58741 taccttaact gtatcaccat atttttcacg aatgaaatca agtgttattt tttgccactc
    58801 agaaaaacct tctgaagaat ctatacataa aacatcagca ccagcttcaa ctaaagcagg
    58861 aacacgttca gcaaagtctc tagtattaat accagctcca actacatatc tcttagaaga
    58921 atctaaaagt tcaagtggat tttctttatg agaagagtaa tctttacgga ataccatata
    58981 cataagatta ccattatcat caagtacagg aagagaatta agtttgtggt cccatataat
    59041 attgtttgct tcctttaaag taacatcttt gttagcataa actatagatg acataggagt
    59101 cataaattca ctaactttag tatctaaatc catacgactt atacgataat cacgacttgc
    59161 aacaataccc aataatttac cagttgaagt tccatcttca gtaacagcta ctgtagaatg
    59221 tccagttttt tcttttaagt caagtatgtc ttttaatgtg ttatcaggtt taatatttga
    59281 atcacttaca acaaaaccag ctttgtggga tttaacacgt gctaccatag cagcttcatt
    59341 ttcaatagtt tgagaaccgt atataaatga aataccacct tctttagcaa gtgcaatagc
    59401 cattttgtca tcagaaacag attgcataat agcagaagtc aaaggtatat tcatataaat
    59461 atctgcttcc tcgccctttt taaatttagt aacaggtgtt tttaaactta cattagcagg
    59521 tatacaatca gtagaagaga aacctggtac aagaaggtat tcactgaaag tatgtgatgg
    59581 tgtatcaaaa taaaacgcca ttttttaatc actccttata tttttatact ttttataatt
    59641 gatagtatta attataactt attctttaat tttattaaag tattttgtca aatattgaga
    59701 tttttgaaaa atttaaacta ttttaattaa atttgtttaa tatatattgc cttatagggt
    59761 aaagttgtat attaaataat gtttagtgtt taatagtagc taatttaaaa ttaatcttta
    59821 gtgattcata taattacatt tgtatagttt acattgataa tattactaga gagtgcataa
    59881 acctcaaaaa tacagataaa ttaaatctat attagtatat ccaaaagtac atttaataaa
    59941 tttatttaac tgaattatta ttttcatttc tattaaattt aaatatttta taaaaatagt
    60001 attatttaca aaatcatata ctccaaaata ctacataaat aaaattaaac aaaaactaaa
    60061 atgacaaatg tcattatcta aagtgacata gaaacggttt cttaaagttg ttactataaa
    60121 atataaactc tataataaaa gtataacaaa agggaatcat atcgatatag aatataattc
    60181 aagaggaggt aaaattatga gtagttctaa tcaatcgaca ataaagacat ctatacagcg
    60241 agtagggaaa tttttaagtg gtatggtaat gccaaatata ggagctttta tagcatgggg
    60301 tcttataaca gcattattta taccaacagg atggatgcct aatgaaaaat tgagtactat
    60361 aggtgaccca atgataaaat accttttacc actattgata gcttatactg gtggtaaagc
    60421 aatagcagga caaagaggtg gcgtaatagg tgcagcagca gctatgggag taattgttgg
    60481 tgcagatata ccaatgttta ttggagcaat gataatggga ccatttgcag gatgggtaat
    60541 taagaagttt gataaatttg tagatggaaa gataccaaca ggatttgaaa tgttagtaaa
    60601 taacttttca ataggtataa taggtatgct tcttgcaata ctaggatttt atgcaattgg
    60661 accagcaata gtagcaggga cagctcttat agaatctgga gttcaattta tagttagcaa
    60721 aagtttattg cctctagttt cagtatttat agaacctgga aaagtattat tcttaaacaa
    60781 tgcaataaat catggtatat tagggcctat aggtatagct gaggcaaaag aagcaggtaa
    60841 atctataatg ttcttactag aaagtaaccc tggaccaggt ttaggagttc tattagcata
    60901 ttggatgttt agtagaggaa gtgtaaaaca atcagcacca ggagctgtaa taatacattt
    60961 ctttggtggt atccatgaaa tttacttccc atatatttta atgaatccag ttttaatatt
    61021 ggctacaatg gcaggtggag cagcaggaat attgactttt tcaatattag gagctgggct
    61081 tgtagcagca ccatcaccag gaagtatatt tgcacttatg gcactagctc caaagggagg
    61141 attacttcca gtattagcag gagttgcagt ggcaacagtt gtatcattct tagtggcagc
    61201 accatttgtt aagagagctt cagcaaatca aagtgaagaa gacagtactt ctttagaaga
    61261 agctaaagca aaaatgagtg atatgaaatc tgcaagtaag aactctgaga aaaatattga
    61321 ggaaaaacag cttgaagtaa atgagattaa gaagattgta tttgcatgtg atgcaggaat
    61381 gggttcgagt gcaatgggag cttccagatt taaaaataga ataaaaaact tagacttaaa
    61441 tatagaaatt acaaattcat ctgttgataa tcttccagat gatacacaaa tagtagtaac
    61501 tcacaataca ttagttgaaa gagttgctaa gaataattca agtgttgaga tagtttcaat
    61561 aaacaatttt ttaaatgacc caaatttaga tacattgttt aaaagattag aatctaaata
    61621 aaaataaaaa gtaccactaa ttaggagtgg ataatttgaa aaaaaagaag attacatcta
    61681 ggcaaaaaaa gataatacta atgatagtag caaattcaaa gaaaaacatt ccaataacta
    61741 tttctgagat tgcagggact ttagagttaa gttcaagaac tgtacttaga gatatgtctg
    61801 gaattgaaaa atggtttgat gaaaatgatt ttaactttgt aaaaaaacca ggagtgggat
    61861 tgattttaga ggaaaatata gaaaatcaaa actttataat tgagttgtta gaagaagaaa
    61921 aaatagaaaa agaatacagc aaggaagaaa gaaatttaat tatattatca aagctactag
    61981 tttctaacga gccagtaaaa tcctattatt ttgcaaaaat attaaaggta tctgaatggg
    62041 tactgaataa tgattttgcc ttagcaagta aatggttgga aagatttgat attgagcttg
    62101 ttagaaaacc agggctcggt gtctacttaa aaggtcaaga aaaaaacttt agagaggcat
    62161 atgtaaatct tatatacgat tcattcaatg aaaaagaaat actagatatg gtcagaaata
    62221 taagtgaaaa tatacaaaca gataaagcaa tagaaatttt aagtgaaaat agacttttaa
    62281 atttaatgga caggtgcata ataagaaaag ttgaagctac attaacaaag aagctttcag
    62341 atttagatgt aaatttagct gatagtgcat atatagggct tgtagttcat atttcattag
    62401 ccttacagag gataaaaaat ggtgaaaata taactatgga taaagagttt ctaaaagaat
    62461 tatctataac agaagagttt aaattagcta aagaaatcgt aaaaggtatg gaaacagatt
    62521 ttaacatgga tataccagta gatgaggttg gatatataac tatgcatata agaggtgcaa
    62581 agcaaaggtc atcttcaaac cataaggctt taaacttaga tgatatagaa ataatggaaa
    62641 ttacaaataa gatgatagat ttggcggaag atgaatttaa aataagttta aaaaatgacg
    62701 agaggttatt taaagaccta gcaaatcact tagggccttc tataaatagg ttgaatatgg
    62761 gattagaaat aagaaatcct cttttagatg aaataaaaag caagtattca tatgcctatg
    62821 atgtagttga aaagatttct agaattataa aagataaact aaatataaat tcaatacctg
    62881 aatcagaaat aggatatatt gcaatgcact ttgcatcagc catagaaaaa aatcttatga
    62941 tgaatactaa tataaatatt gttgtggcat gtcccactgg aataggtact tcaagattct
    63001 tatcaactaa gattgaaaat aaatttccta atttaaatat acttgaaacc atatcagcta
    63061 taaacataga tgaagagtat ttaaaggaaa aagatgttga tttaatagtg tctacagttg
    63121 agttaaatac aagtttaaat tatatatgtg taggtccatt tatgagttta gatgatgaac
    63181 agataatcaa ggaaaaaata aaatctatag ctcaaaataa attaatcaat ttaaatgcga
    63241 aaaatgatac taagagtaag aataaagtat atgaacagat aacagaaagt atgaatatag
    63301 gaaaagatat tcttcaattt ttagaggaaa ttagatttga aaaatttgaa agtaaagatt
    63361 taagtgagct tattgaagac tcatcaaaaa tatttgcaaa atctagtgaa gatataatct
    63421 ctataaaaga atcgttaaaa gaaagattaa aaatttcaat tccatatata gaagaatcaa
    63481 aaattctatt gttacattgt atgagtgaaa gaatagatat tatgaagtta tcaataataa
    63541 aactagagaa tagaatcgtg ctagattcaa atgaagaaat cgacaatgta gtttttatgt
    63601 tacttcctaa aaattcacca agttatcaaa gacaaataat gagtgaaata agtggttcat
    63661 taatagacaa tataattttt actaataaga taaataaatt ctctatagaa gagatggcat
    63721 tagaggtaaa agatatagtt tttaattttt atacaaacag attaaaagca tttatagata
    63781 aataaaatta atattaaaaa ggaaggttga tactatgagt aaagtattga atgagaataa
    63841 tatattttta gggttagata gtgtttcaaa ggaagaagct ataactttag caggaagaaa
    63901 attagttgaa aatggatatg taaaagaaga atatatacca gcaatgttag aaagagaaaa
    63961 agtaatgact acatatatgg gaatgggagt tgctattcca catggtgtaa atgaagcaaa
    64021 aaaagaaata ttatcttctg gaatagttat attacaattc cctaatggta tagattttga
    64081 tggagaaaaa gcatatttat taataggaat agctggtgtt ggagatgaac atttagaaat
    64141 actatctaat atagcagtag ttttagatga tgacttaaca gaaagactaa aaaattcaaa
    64201 tgacaaacaa gcttttatgg aagcttttgc caactaatct taggagggat ttaaaatgaa
    64261 aaaggcaatt cagtttggag caggaaatat tggaagaggt tttattggag gcttgttagt
    64321 taagtcgggt tatcatgtag tatttgctga tgtaaatgag gagatattaa actcaataaa
    64381 caaagataaa aagtatacta tacacataag agatgtagaa tgtatagatg aagtaattga
    64441 taatataagt gcggtttcat ctataaaaga agaaataata gatgagattg tacaggctga
    64501 aataataact acagcagttg gacctttagt tttgactaaa atagcctcta caattgcaaa
    64561 aggtataaag gctagaaaag aaaagggatt aacaagtaat ctaaatataa tagcatgtga
    64621 aaatgcaata tacgcaagtt cttctttaaa agaagaggta cttaagtatt taaataagga
    64681 agaagtagaa tatttagaga tgtatgttgg ttttccaaac tgttcagttg atagaatagt
    64741 tcctcctggt aaaaacgaaa atcctcttga tgtaacagtt gaaaattttt atgagtggaa
    64801 tgttgaaaaa caaggattta aaggggaaat accaactata gttggtatga atttagctga
    64861 taatcttatg gcttatatag aacgtaaatt gtttacactg aatacaggtc atgctataac
    64921 agcttatata ggatatttaa aaggatataa gactatagaa gagagtataa aagataaatt
    64981 tatctgtgat atagtcaaaa gtgcaatggt agaaagtgga gaaggattga ttaaaaaata
    65041 caattttgac tcagaagtgc actataaata tattgataaa attttaaata gatttaaaaa
    65101 tccatatctt aatgatgatg tattaagagt cggaagggaa ccattaagaa aactaagtga
    65161 taaagataga ttgattaaac ctttaatgac agcaaaatca tatggattat cagttgataa
    65221 tttgattctt ggaataggtg cagcgcttca ttataataat agtgaagata ctcaaagtgt
    65281 agaattacaa gagttgataa agtctattgg agtaaaaaaa gccgttgcta aaatagctaa
    65341 tataagcaat gacgaagaac ttttaaacaa tatcgaaaaa agttatatat ttatgaaaaa
    65401 tttataaatt tacttgaaaa aaagtagttc tgataaagta actataatat attattctac
    65461 acagtttgaa ttatttgttg aataatagag aattataata tataattaaa aggaataaat
    65521 taatatattt acctgtatat gtttgataat atggttcaaa tgtttctact aagctaccat
    65581 taatagcttg actacaggag tgaagaagat atagtttata cttattttaa attatatatt
    65641 aagttaatta ctttgaattt taagctcctg tctatgatat agacaggagc ttttttagta
    65701 ttttaatata aaatacttac aagaatagat agcaaaatta gaacaaatat aattttttta
    65761 ttaatataat tttagaaagg gctaggtgga agttcaatga aggctttaca agaaaagata
    65821 ttgagagaag gcagtgtttc aggaaatgat atactaaaag ttgatagttt tttaaaccat
    65881 caaattgatg tagctttttt aaatgaaata ggaagagaat ttaaagagag atttaaagga
    65941 gaaaaggtgg ataagatatt tacaatagag gcttcaggaa tagctatagc atcaattgtt
    66001 tcacagtatt ttgataatgc acctgtagta tttgcaaaaa aatcagaatc taaaaatttg
    66061 gataaagatg tatatgaaac taatgtatat tcatttacta aggcaagaga gtattcagtt
    66121 aaagtttcta aaaagtacat aaacaaaggc gaaaatatat tgatagtgga tgattttttg
    66181 gcaaatggaa gagctgctct tggacttaaa gatttaattg agcaagcaga agctaactta
    66241 gttggagtag gaattgtcat tgaaaaagga tttcaagctg gaggagcact tttaaaggct
    66301 aatgatgtaa gattagagtc tttggcagta gttgaatcta tagataatgg aactgtaaaa
    66361 tttaggtaat ttatgatatt atatacttgt gtttagcaca tattttaact atgtaattat
    66421 ggaggatttt ttatgagatt ttttatagac acagccaata ttgaagaaat aaaagaagca
    66481 aatgatttag gtgtaatctg tggtgtaact actaatccgt ctctaatagc taaagaaggt
    66541 agagatttta tagaggtagt taaagaaata agtgaaatag tagatggacc aataagtgca
    66601 gaagtaataa gtttagaaca taaaggtatg atagaggaag cagaaaaatt atctaagata
    66661 cataaaaata tagttataaa gatacctatg acagcagaag gtctaaaagc ggtgaaagta
    66721 ctatcaagta aaggtataaa gactaatgta acgcttatat tctctgctgg acaagctctt
    66781 ttagctgcta gagctggagc tacatatgta agtccttttg ttggtagatt ggatgatata
    66841 tcacaaaatg gtcttgattt aattgaagaa atcgttgata tatttagtgt aaatagtatt
    66901 gaggctcaaa taatagttgc tagtgttaga aatccaatac atgttttaca agctgctaga
    66961 atgggagcag atatagcaac agttcctctt aaagttataa accaaatgat taagcatcct
    67021 ttgacagaca aaggaataga tagctttatg aaggattggg aaggtgcttc tttaaaatta
    67081 taattataaa ctacgcttta taagtatatt aaagaggtta ttatggaaat tctaagtaat
    67141 aaggctaggc tattttgcct agtcttattt tggtgtataa tatattgaaa catattattg
    67201 aaatagatta attttttaag tgaggaggcg aaaaatggat aaaatattaa gagagataca
    67261 aagagaagat aaaaaaaatc catatactga ccaagaactt gctgaaatgt taaatgtagc
    67321 taggtctgaa gtgatatcag ttaggaaaaa aaataatatt ttagattcga gagaaagaag
    67381 aaaaaaaata ttaataaaag atatatcaaa aattgtaagt gaaaatcctc aaatgtctga
    67441 aagaaaaata actgaaatat taatagaaaa tggatataat atatcgagaa gtgcagtttc
    67501 aaaattatta aaagaggaaa atttaaatca gtcaaagatt gggattaagg ataaagaccc
    67561 agcatcatca ggaaagtatg tgaaggtgag taaagttgta gaagaagatg gatttgaaga
    67621 attgataggt ataaagggaa gtttgaaaga aaaggtaaat ctagctaagt cagcaataat
    67681 gtatcctcct aatggccttc atactataat atatggagaa acaggagtag gtaaaagtga
    67741 attagctaca tgtatgtata aatatgctat aaaaaacaac attaaagaag agaacagccc
    67801 atttatagtg tttaactgtg cagactatgc agaaaatcct aatctattga tagctcaact
    67861 ctttggggta gtaaaaggtg catatacagg agcagatgca aatagagaag gtcttgtaga
    67921 acaagctaat aatggaatat tgtttttaga tgaaattcat agattgcctg ctgctgggca
    67981 agaaatctta ttttcattaa tagatagagg agaatttaga aggttaggag aaagtagttc
    68041 taatagaaag gcaaatgttc tcataataag tgctacaaca gaaaatccag attctaattt
    68101 acttcaaaca tttagaagac gtataccaat ggtaataaat ttacctaata tgagtgaaag
    68161 accaaaatct gaaagatatg aaataataat aaagtttttt gaaagagaag caaagcgagt
    68221 aaacaagaat tttataataa ctaaagaagt tatgtgtgca cttatgaatt ataagtgtac
    68281 tggaaatata ggtcagttaa agagtgatat acaagtaact tgtgctagag cttttagtaa
    68341 atcgatattt tctagcgata aagtaattat agatttagat agtttaaggg attatataaa
    68401 aagtggatat tatgattgta attgtaaaaa tgaaaagtat tctgagtttt cagttgagga
    68461 tatatatata gatattagtg aattggtgag tggaaaaaat agaattttat ctgataatga
    68521 gataagcgag atatataatt atgcagaaaa agagcttaag gttttggaaa gtaagtgctt
    68581 ttcagaagaa gagttaaaaa atgcatttat agaaaagtta gataagaagt ttaatgctat
    68641 taaaaacaat aaaaatttat ctgatagaag aagtcgaata gactggggag gacaattaaa
    68701 agagtctaca ctggagataa tggataaggt aattgtattt attaaggagc agtttaagca
    68761 tgtaaatcaa ggtctgtact tggctctagc aattcatatt gagcatgcta taggtaggat
    68821 aaaagatgga aaaactataa taaatccgag tttagataaa ataaaagcat caatgccaat
    68881 ggaatatgag ttgtcaagat atataatggg tatagtagaa gatttgacac aagtaacatt
    68941 tccagaagat gagcttggat atttggctta ttatataaat aaattttgtt ataatgagga
    69001 aagtataaaa gataaagtta aagtagtaat agttacgcat ggtaaagttg gaattgaaat
    69061 gtctaaagtt gtaaatcata ttttaggtat agaatgtaca ctaggtatag aaatagcact
    69121 tacagattca ccatcagagg ggatagagca tgtattagaa gaacttcaaa agatagaagc
    69181 tagaaaaggt attctagttc taattgatat gggttctttg gtaattcttg gtgatgaggt
    69241 tgaaaaaagg cttggaatta gatgtaaaac tgtaaataga gtagatacat tattggcaat
    69301 ggaagcagga aaacttgcaa caattgaagg aaaaagttta gatggtataa tagctgattt
    69361 aaagaaaaat aagaattatg ctatgataaa tacaaataaa tttagttaca gaaaaaacga
    69421 gtatggaaag aaaaatgtaa tcattacttt atgtttgagt ggtgtaggta cagctttaaa
    69481 tttaaaagag catatagaaa aacaaattaa ggaatatgac actttaattg aagtaaagcc
    69541 agttgctttt ttaaataata atttagaaga agagttaaat gatattgaat ctgaatataa
    69601 tattattgct atctgtggaa ctattgatat agattataaa aacgtgcctt ttatttctta
    69661 taatgaggta cttggtaaaa atggtattaa taaagtatta gagcatttaa atagtaaaca
    69721 accagcttta aatattgaaa accagttgaa taatttaata catgaagatt tgattttata
    69781 caattttgaa ggaatttcaa aggagtatat aattgatact cttgtttcaa aacttgaaga
    69841 aggtggatat gttgactcaa agtatatatt aagtgtatat aaaagagaag caatgggaag
    69901 tgttgtaatg gcctctaagg tagccgtgcc acatggactt ccagaaaacg ttatgaagcc
    69961 tgcaatagca atcgcgaggt tgaataaacc tatagtatgg gacaataaat ttatggtgga
    70021 tttggtggta ctactggcac ttaaagaaaa taataagaaa gaaatacgaa gcttattttc
    70081 aaagattaat gaccagcata ctttagaagt tttattaaat actgaagata aaaatgaaat
    70141 aaaagaactt ttaagctaat tattagtacc gagaagaaag acaaaaaaat ttgctttagt
    70201 ataaaattta aaactactca attgctaagt agttaattac tctatataag gctagttcta
    70261 tgaaatttat ttatatgatt ttatagaatt agcttttttc ttggtaaata aaatatataa
    70321 atttatttat gtaaaaagta aattttattg gacaattcta taaaaatttt tcctatattt
    70381 cttataaaat atagaatata taaaaaatat ataaaaataa attaaattca taataaataa
    70441 ttttataaaa tagttgttaa acataaaaaa ccatttgtaa aaaacaaaaa aatcatttct
    70501 ctaacaaaaa aaaccacaaa aaatataaaa aagtatggct agttaagtga ttaacaagta
    70561 aaaattaaaa taaaaatcaa aaaaaattta tcaatgaaag tcagctaaaa tcctggaaga
    70621 gagcattatt tttgggaaaa cattttcttt tgtttcacaa aaaaagccac tttttggcac
    70681 acaacatgct ttatataaag tcaagaaaaa ctaaggtata agacaaaggc aattaggagg
    70741 tgtaaatatg gagtttataa attttgtaag tgataaagtt atagacctta aaatggattg
    70801 taaaacgaaa gaagatttct ttaaagaaat acataataaa gtttttgatt taggctttgt
    70861 aaaagaagaa tttggagaaa aaatattagc aagagaaaat gtatttccaa caggtttaaa
    70921 tttaggtgat tatggagtag caataccaca tacagatgcg gaatatatta aagagcaatt
    70981 tatatcagtc tgtactttta atgaaccagt agtatttagt tctatggaag accaagatga
    71041 aaaagtacct gtaaatttgg ctttcgtatt aggacttaat caaccacaca gtcaattatc
    71101 tgtattaaca gaattaatgg gaattatgca aaataaagag ttagttgata agttgatgag
    71161 ttctactgat aaagaagaag ttttaaagac actaaagtct ttataaaaat aaaaaagtac
    71221 taaaaataaa aaatataaaa ataaaaatta ttaggaggaa ataaaaatga aaaaaatatt
    71281 agtagcatgt ggagcaggaa tagcaacatc aacagtagtt tgtgacaagg tagagagatt
    71341 agttaaggaa aataatatac aagcagaagt tatacaatgc aaaatagctg aatgtgcaac
    71401 aaagcaagaa ggagcagatt taatagtatc aacaactata ctaccaacaa cttataatat
    71461 accagcaata aaagcaacag catatataac tggagtaaat acagcagcat tagataaaaa
    71521 aatattagat gctttaaaat aatcctatca tagatacatt gaaataatat tttataaaac
    71581 agaaaaagga ggatttataa atggataaat tattagcagt agtacaatac attttaaata
    71641 tgggaccgac agtaatacta ccaataacaa ttgtagtact aggaatgata tttagaatta
    71701 agtttacaaa agctttaaag tcaggtctta caattggtat aggatttgtg ggtataaact
    71761 tagttgtaaa cctattaaca tcaacactag ggccagcggc tcaaggaatg gttgaaagat
    71821 tcggacttaa tttaacagta atagatgttg gttggccaac aacagcatca gcaacatggg
    71881 cttcaccagt tgcaccaata ttaataccag tttgtttatt agtaaactta ctattattat
    71941 acttcaataa aacaaagata ttaaacgttg atatatggaa ttattggcac tttatagtag
    72001 caggtgcagt tggatatata gtaacaggaa gttatgtttg ggctatattc tgtgcaatac
    72061 taatggagtt attagtactg tttattgcag aaaagacagc aaaaaatgtt caggagtttt
    72121 atgatttaga aggtatagca ttaccaactg gttcaacagc ttcatttgct ccacttggat
    72181 atttagtagg taaatttgtt gaaaaaatac caggaataaa taaaatacaa gcagacccag
    72241 actctataca aaaaagattt ggtatatttg gagaaccaat gatgatgggg attatactag
    72301 gtataatact tggagcactt gcgggatatg atgtagcagg aatattcaat gtaggtatat
    72361 caatgggtgc agtaatgtta cttatgccta gaatggttag aatattaatg gaaggtttaa
    72421 ttccaatttc agaatcaata agagatttct tacaagcaaa atatggagac agagaacttt
    72481 atataggtct ggatgcagca gtagcaactg gacatccagc agttatatca acagctctta
    72541 tacttgttcc tataactttg ttcttagcag ttatactacc aggaaacaaa gttttaccat
    72601 ttggagactt agcgacaata ccattttata tggcatttat agtgtgtttt agaaaaggta
    72661 atatagttca atcagttata acaggaacaa tagtaatagc tatatcactt tatatggcta
    72721 caaactttgc agatgttcat acacaattat tacaacaagc aaatatgatg ccagaaggaa
    72781 caacaatggt tacaagtata gatacaggtg gaaacttgct aaaatgggga atattgaaat
    72841 tatctcaatt agtttcttca tttggaatat tctagtatgt agttaataga gattactctt
    72901 ttttagtaaa gtatgcgaaa atttaacatt tagcagctac taaaaaagag taatattttt
    72961 tataaatttt taaaaataca aaatacaaga ggaggagatt tatcgtgaaa gcagcagtat
    73021 tacatggcac aaatgatatg agatttgaag atatagaaat aaaaccatgt gaaagtgatg
    73081 aagtaaaaat aaaagttatg gcagcaggaa tatgtgggtc agatcctcca agagtattaa
    73141 aacattggaa atatccagtt ccagctatac caggacatga gttttctgga gtaattgctg
    73201 aagttggtaa ggatgtaaaa aatgtaaaag ttggagatag agtagttgca attcctttta
    73261 taccttgtaa tgaatgtgaa tattgtaaaa gaggattatt ttcattgtgt gatgaccatg
    73321 gaatgttagg agctaaaagt tttggagctt ttgcagagta tgtaaatata aaagctacaa
    73381 atgtacttcc aataggagat atgaactttg aagatgctgc aatgatagag ccactagcag
    73441 ttgctatgca tggagtactt aatataggtg ttcaagttgg agacactgta gctgtaatgg
    73501 gtagtggaac aatggggcag ttggtaatac aaggacttaa aatagcaggg gcaggaacaa
    73561 ttatagcagt tgatatatct gataataaat taagagaatc aaaggaatta ggggctgata
    73621 taataattaa tgctaaagat attaacccag tagaaaaaat aaaagaactt actggtggaa
    73681 aaggtgttga catagctctt gaatgtgcag gttcaaaaat aactcaagaa caatgtttac
    73741 ttataactaa aaagaaaagt aagataggat ttttagggat agcatattca gatataactt
    73801 tatcagaaga agcatttgaa aatatattta gaaaagaact agaattaaaa ggtttttgga
    73861 actcttattc tgcaccattt ccaggacaag aatggactaa gggaatcaat ctagttaatg
    73921 aaggaaagat aaaattaaaa gaaatggtat ctcatagatt ctcacttgaa gatacttaca
    73981 aagcatttga aatgataaga gatagaaaag aagaatttaa caaaatatta attttaccac
    74041 aaggggttga aaaataatga aagcagtagt taaaactaaa ccaggctatg ataatgtaga
    74101 agttttagaa gtagaagaac caaaagctac aggagacaag gttaaaataa aagtagaata
    74161 tagtggtata tgtggttcag atatacattc atttaaaggt gaatatgcaa atataaaagc
    74221 cccagtaaca ttaggtcatg aattttctgg aattgttgta gaagtaggag aagatgttaa
    74281 aaatataaaa gtaggagata gagttacatc agagactact tttgcaactt gtgagaaatg
    74341 tatatattgt gaaactaaag attataactt atgcccaact agaaaaggta ttggtactca
    74401 agtaaatgga agttttgctg agtatgtatt atcaagagaa gaaagcatac atgtacttcc
    74461 tgaaaatgta tctttattat cagcagccct tacagagcca ctagcttgtt gtgttcatgc
    74521 agctcttgaa aagactacta tagaaagttc tgatacagtt ttaataatag gaccaggacc
    74581 aattggttta ttattagctc aagttgttaa atctcaaggt gctacagtta taatgtctgg
    74641 tgttacaaaa gataaggaaa gattagaaat agctaaaaat cttggagtag atagaactgt
    74701 aaatgtaatg gaagaaagtt tggcagatgt agtgaatgaa atgactgatg gaaatggagt
    74761 aaacaaagga tttgattgtt ctgggtttat gccagcagtt aacgaagcct taagactgct
    74821 tagaaaaaaa ggtactttcg tacaagttgg aatctttgct aaaaaattaa atgaaatgga
    74881 ccaagaagca ataatccaaa gagagttaca atatatagga agcaggtctc aaaagccaag
    74941 ttcttggatt acagcattag atttaatggc aaatgggaaa gtaaatacag aagctttagt
    75001 tactagaact gttggtttag acgaatttaa agaaggtatt acagctttaa tgaacgggga
    75061 agaaataaaa gtagcagtaa aatcttaaaa aaacatttgt attattttaa aaaaaattgt
    75121 aaaatctact gtaggataaa aaaatgtaag gtcttaaaaa ataacaggag gaagaaagta
    75181 tgagcactct tagagatcat tctaataata ttagaaaaaa cataataaaa atggttgcag
    75241 aagctaaaag cggacatcca ggtggttcat tatcaatagc tgatatgttg acagtattat
    75301 attttgataa gatgaacgta tgtgcagaaa atccaaaaat ggctgataga gatagatttg
    75361 ttctatcaaa aggtcatgca gcaccagctt tatatgctac attagcagaa aaaggatttt
    75421 tcccagaaga agaattaata acactaagaa aatttggttc taagttacaa ggtcacccag
    75481 acatgaaaaa agtagcagga atcgatatgt ctactggttc tcttggtcaa gggctatctg
    75541 cagcaaatgg tatggcactt gcaggaaaat tagataacaa agactataca gtatatacta
    75601 tattaggtga tggagaaatc caagaaggtc aaatatggga agctgcaatg acagcatcac
    75661 attacaaatt agataattta attgcatttg ttgacttaaa tggattacaa atagatggtt
    75721 ctaatgaaga agtaatgaat gtaagtccag tagatgacaa attcaaatct tttggatgga
    75781 atgtaatagt aataaatgga cattcttttg aagaaatagg aaatgcagtt gatgaagcta
    75841 aaaaagttac aggaaaacca actgtaataa tagctaaaac agtaaaaggt aaaggtgttt
    75901 catttatgga aaataatgct gcatggcatg gaacagctcc aaatgcagaa caagcagagc
    75961 aagcattgaa agatttagaa ggaggtctat aggaatggga atagcaacta gagaagctta
    76021 cggacaagtg ttaaaagaac ttgcagaaaa taaagatata gtagtattag atgcagattt
    76081 aggaaaagct acaaaaagta tatcatttaa agaagttgcc ccagatagat tttttgacat
    76141 ggggatagca gaaggtgaca tgattggtac agcagcaggt cttgctactt gtggaaaaat
    76201 accttttgcg agtacttttg caatatttgc agcaggaaga ggctatgagc aaataagaaa
    76261 ctctgtagca tatccaaatt taaatgtaaa aatagcagct actcatgcag gtgtaacagt
    76321 aggtgaagat ggtggtagcc atcaagctat agaggatata tctctaatga gaggtatacc
    76381 aaatatggtt gtattaaatc cagcagatgc tttagaagca agacaagcta ttcttacatc
    76441 tattgattat aatggacctg tttatataag attaggtaga gctgcaactc ctgatgtaaa
    76501 cagtgaaaat tataaatttg aaataggaaa aggaactgtt ttaagagaag gttctgacat
    76561 aacagtaata gctacaggaa taatggtagc aaaagcctta gaagcagcag aagaacttgc
    76621 taaagaaggt ataaatgtag aagttgttaa tatatctaca ataaaaccat tagatgaagc
    76681 attaataaaa gaaactgcta agaaaactgg aaaagtagtt acagcagagg agcacagtat
    76741 aataggtggt ttaggttcag ctgtatgtga agctttagca gaaactaaag atgtagtagt
    76801 tagaagaatt ggtgttaaag atgtatttgg tcaatcaggt acaccagctg atttattaaa
    76861 acactatggt ttaactacag aagatatagt aaaaaatata aaagagttgt tatagatata
    76921 aatatatcta aataacaaat tgatttgaca taagtcaatt aaagctgtct taaaaataga
    76981 aagaaatttc tacaatggga cagctttatt tttacaatac tttattttta tgataattat
    77041 aaaatagtct gaaagtaatc aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc
    77101 tattggatgt gaccatggtg gttatgaatt aaaggaatat ataaaggaaa atttaataaa
    77161 taaaggtata gaagttgaag atgtgggtac aaattcaaaa gaaagtgtag attttccaat
    77221 atatgcaaaa aaggtaagtc aaaatgttca aagtaaaaaa tctgatttag gcatactttg
    77281 ttgtggaaca ggaataggta tgtctattgc agcaaataag tttaaaggaa tcagagcagc
    77341 tgttgtttct gactgttttt cagctaaggc aactagagaa cacaataata caaatgtact
    77401 ttgtttagga gaaagagtta ttgggaaagg attagcaatg agaattgttg aagaatggtt
    77461 aaattcaagt tataatggag aaagacatgc aagaagatta caaatgattg atgatataga
    77521 aaaagaaaat ttataaatat ataatttata acaaggggga gcgttatgtc tattatttgt
    77581 gcttcaataa tgtgtgcaga ccaaatgaag ttaaaagagg aattagaagc tcttgaagaa
    77641 gcaggtgtaa aattacttca ttgtgatgtt atggatggaa tatttgttaa aaacttggct
    77701 atgggaccag agttattaaa atcaataagt gaaaatacaa caataccttt agacatacac
    77761 ttagccacag aaactccaga taaatatatt gatatgatgt cttatataaa gccaaaatat
    77821 atttcttttc atgtagaatc aagtacaaat gtaaaagctg atatacaaaa gttaagaaat
    77881 tatggtatag ggccagtgtt agcaataagc cctcaaacaa gtgtggataa aatagaagaa
    77941 tatataagtt tagttgaggg tatcttagta atgacagtaa atccagggtt tgcaggtcaa
    78001 aagtttaatc tttctgtatt ggataagttg gataaactta cagagatact aaaagattat
    78061 gataatcctc catttataga ggttgatgga aatataaata aggatactat taaacttatg
    78121 aatggtaaaa aagtagatat atatgttgtt ggtacttcag ctttatttaa tgataagcct
    78181 ccaatttcat ataaagataa aatagaagaa ctaaaagaaa gtataaagta accatgtaat
    78241 aaaattataa tatgtttctg tttaatagta aaaatttagg tggaaacaag aaattttata
    78301 atatttaata aaaattttgg ttgagtaagg agaatttttt atgggagacc tatataaact
    78361 acaaaatggt actgatatta gaggaatagc gtatgaaaat gattctaaag aagttaacct
    78421 aactgtagaa gaagtaaaaa aaatagctaa agcgtttcat atttggttaa aagaaaagac
    78481 taagaaagat aaagtaactg ttgccatagg aactgattct aggataacag gaagccaatt
    78541 tagaagtact gttattgaaa ctttaactaa tgacgattgt aatgtaattg attgtgagat
    78601 tgccacaact ccagctatgt ttatgacaac tattatggat ggatacaatt ctgatggggc
    78661 gattatgatt acagcaagtc acttacctta ttactataat ggtttaaagt ttttcacaga
    78721 aagtggaggt ttagaaaaaa ctgatataaa agagatgttg gatatagctg ttaaaaatga
    78781 tagtgtagac tatgaggaag taaataaaaa aggtgaagtt atagtaaaaa atttaataga
    78841 agattattct aatttattga tagataaaat tagaaaaggt gtaaattcaa gtaaaaatta
    78901 tgaaaagcct ttttctgggc ttaagatatt ggttgatgca ggaaatgggg ctggtggatt
    78961 ctttgctgaa aaagttttac acatactggg agcagatacg acaggtagtc agtttttgaa
    79021 cccagatggt atgttcccaa atcatatacc taatcccgaa aacaaagaag caatggaaag
    79081 catatgtaaa gctgtgttag ataataagag cgacctagga ataatctttg atacagatgt
    79141 agacagagct gctattgtag gtaaaaatgg caaaccaata aataaaaatg ctcttatagc
    79201 tgttatatca tcaatagtat tagaagaaca tccaaaaaca gctatagtaa cagattcgat
    79261 tacttctgaa ggacttgcta aatttataaa tgaattacaa ggaagacatc atagatttaa
    79321 aagaggatat aaaaatgtaa taaatgaagc tataagattg aatagtgaag gtgaagagtg
    79381 ccatttagct atagaaacgt ctggacatgc tgctttaaaa gaaaattatt tcttagatga
    79441 tggggcatat ttaatagcta aaattttaat aaaagtagca aagctatcct tggaaggaaa
    79501 gactatagaa gaattgatag aaaaccttga agaagctcaa gaagagaaag aagttagaat
    79561 aggcattaat aaagaagatt ttagaccata tgcagaggat atattaaaag atttagattc
    79621 tcatgtcaag agtattgatg gatggtctgt agcaccaaat aattttgaag gcataagagt
    79681 taattgtgat gaatcaaatg gagatggatg gtttttactt agaatatcac tacatgaacc
    79741 attattagct ttaaatattg aatcaaataa aattggcgga gcagaacaaa tttattctaa
    79801 gttaaaactt ttcttagaaa aatatgattt aaaaggtctt tagggtaaat agaaagaata
    79861 tatttatatt gaagaataaa tgtaaatttg tacctaagat tacaaatatt acaaaattgt
    79921 aatcttaggt aacagagatg taactgatta tggaaacact atgtgatatt atagatacag
    79981 agagatgaaa tatgtgatga attatagaga aatataattt aaaatgtatt aatatcacac
    80041 caaaaaggta taatcaaaaa atatgtaaat ataaacccca acatttagaa gaagacctct
    80101 aaggaggttt ttttcttgtt taaataactt aaaatatatt tttatatcaa tatatcaatt
    80161 aactcttcta aaagtaagca ttaccttaaa taaatcagca tctataatta tttccatgtg
    80221 tgcattttct aattcaatta tattttgagc aattgataaa ccaagaccag agccatctgt
    80281 ttttctagat atatcacccc ttctaaatct ttctattaac tcttctggct ttagatttag
    80341 tttatcattt gaaatatttt taaacgttaa tattatattt tcttccttta taaacatatc
    80401 tatgtagacc ctagtgttag gcatagagta ttttagtata ttgcaaatta gattctcaaa
    80461 aactctccaa gtttttttac catctatcat ggtaaaaaca gcatattcag gtgtttctat
    80521 tataaaatct aaactgctat tttgaatttt gtcttcaaac tctacaatac tttgaattaa
    80581 aagttctctt atatcggttt ttgttatatt aaattctaca tctccactac ttagcttaga
    80641 tgcttcaaat atgtcatcta ttaataattt taatctttgt gattttttat ctaaaatatt
    80701 tatatagtca tttatagtct ctctgtcaaa gtcattattt tttaacatat caacatagct
    80761 taatatagat gtaagtggag tttttaagtc atgagatacg tttgtgataa gctctgcctt
    80821 catcctttca ttttttatat tagcatctac agcagtttca agaccacctg ttatattatt
    80881 tatacttttg gctaaagtag atatttcatc atctcctata acttctaatt tatactttaa
    80941 attgccattt tctatatttt ttatattttt tgatatgtta tttatgtaat taaacttaac
    81001 agataaaaag ttgtatatat aaaaatgaaa aactgtgttt acaagtgtaa ataaaattac
    81061 tatagctatt gcctcagcat aatatatata cataaagaat atcaaaacaa gatattggat
    81121 aatagcttgt acaactatag atatta
//
LOCUS       DAMJYX010000007        77327 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJYX010000007 DAMJYX010000000
VERSION     DAMJYX010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.007"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            776..2446
                     /locus_tag="PC479_000500"
     CDS             776..2446
                     /locus_tag="PC479_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2803088.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2568..3302)
                     /gene="pflA"
                     /locus_tag="PC479_000501"
     CDS             complement(2568..3302)
                     /gene="pflA"
                     /locus_tag="PC479_000501"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2803089.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3462..5693)
                     /gene="pflB"
                     /locus_tag="PC479_000502"
     CDS             complement(3462..5693)
                     /gene="pflB"
                     /locus_tag="PC479_000502"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2803090.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5986..7047
                     /locus_tag="PC479_000503"
     CDS             5986..7047
                     /locus_tag="PC479_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2803091.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7138..8751
                     /locus_tag="PC479_000504"
     CDS             7138..8751
                     /locus_tag="PC479_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2803092.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9026..11011
                     /locus_tag="PC479_000505"
     CDS             9026..11011
                     /locus_tag="PC479_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2803093.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11016..11450
                     /locus_tag="PC479_000506"
     CDS             11016..11450
                     /locus_tag="PC479_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2803094.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11479..12039
                     /gene="srlA"
                     /locus_tag="PC479_000507"
     CDS             11479..12039
                     /gene="srlA"
                     /locus_tag="PC479_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2803095.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12103..12471
                     /locus_tag="PC479_000508"
     CDS             12103..12471
                     /locus_tag="PC479_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2803096.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12496..13062
                     /locus_tag="PC479_000509"
     CDS             12496..13062
                     /locus_tag="PC479_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2803097.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13103..13468
                     /locus_tag="PC479_000510"
     CDS             13103..13468
                     /locus_tag="PC479_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2803098.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13569..14342
                     /gene="srlD"
                     /locus_tag="PC479_000511"
     CDS             13569..14342
                     /gene="srlD"
                     /locus_tag="PC479_000511"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2803099.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14691..16835
                     /locus_tag="PC479_000512"
     CDS             14691..16835
                     /locus_tag="PC479_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2803100.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16923..17258
                     /gene="spoIIAA"
                     /locus_tag="PC479_000513"
     CDS             16923..17258
                     /gene="spoIIAA"
                     /locus_tag="PC479_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2803101.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17272..17700
                     /locus_tag="PC479_000514"
     CDS             17272..17700
                     /locus_tag="PC479_000514"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2803102.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17723..18487
                     /gene="sigF"
                     /locus_tag="PC479_000515"
     CDS             17723..18487
                     /gene="sigF"
                     /locus_tag="PC479_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2803103.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18767..19204
                     /gene="spoVAC"
                     /locus_tag="PC479_000516"
     CDS             18767..19204
                     /gene="spoVAC"
                     /locus_tag="PC479_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2803104.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19371..20384
                     /gene="spoVAD"
                     /locus_tag="PC479_000517"
     CDS             19371..20384
                     /gene="spoVAD"
                     /locus_tag="PC479_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2803105.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20398..20760
                     /gene="spoVAE"
                     /locus_tag="PC479_000518"
     CDS             20398..20760
                     /gene="spoVAE"
                     /locus_tag="PC479_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2803106.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            20943..21959
                     /locus_tag="PC479_000519"
     CDS             20943..21959
                     /locus_tag="PC479_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2803107.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22334..23176
                     /locus_tag="PC479_000520"
     CDS             22334..23176
                     /locus_tag="PC479_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2803108.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23191..23655
                     /locus_tag="PC479_000521"
     CDS             23191..23655
                     /locus_tag="PC479_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803109.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23642..24814
                     /locus_tag="PC479_000522"
     CDS             23642..24814
                     /locus_tag="PC479_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2803110.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            24858..25793
                     /locus_tag="PC479_000523"
     CDS             24858..25793
                     /locus_tag="PC479_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2803111.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26067..28760)
                     /locus_tag="PC479_000524"
     CDS             complement(26067..28760)
                     /locus_tag="PC479_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2803112.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTKVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            28974..29672
                     /gene="yunB"
                     /locus_tag="PC479_000525"
     CDS             28974..29672
                     /gene="yunB"
                     /locus_tag="PC479_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2803113.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(29884..30891)
                     /locus_tag="PC479_000526"
     CDS             complement(29884..30891)
                     /locus_tag="PC479_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2803114.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            31119..32003
                     /locus_tag="PC479_000527"
     CDS             31119..32003
                     /locus_tag="PC479_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2803115.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            32205..33755
                     /locus_tag="PC479_000528"
     CDS             32205..33755
                     /locus_tag="PC479_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2803116.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            33784..34431
                     /locus_tag="PC479_000529"
     CDS             33784..34431
                     /locus_tag="PC479_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2803117.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(34573..34833)
                     /locus_tag="PC479_000530"
     CDS             complement(34573..34833)
                     /locus_tag="PC479_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2803118.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            35050..36333
                     /gene="hflX"
                     /locus_tag="PC479_000531"
     CDS             35050..36333
                     /gene="hflX"
                     /locus_tag="PC479_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2803119.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            36341..37018
                     /locus_tag="PC479_000532"
     CDS             36341..37018
                     /locus_tag="PC479_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2803120.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            37104..37547
                     /locus_tag="PC479_000533"
     CDS             37104..37547
                     /locus_tag="PC479_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2803121.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            37852..38496
                     /locus_tag="PC479_000534"
     CDS             37852..38496
                     /locus_tag="PC479_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2803122.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(38582..38959)
                     /locus_tag="PC479_000535"
     CDS             complement(38582..38959)
                     /locus_tag="PC479_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2803123.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(38959..39345)
                     /locus_tag="PC479_000536"
     CDS             complement(38959..39345)
                     /locus_tag="PC479_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2803124.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            39524..40279
                     /gene="nadE"
                     /locus_tag="PC479_000537"
     CDS             39524..40279
                     /gene="nadE"
                     /locus_tag="PC479_000537"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2803125.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            40362..41081
                     /locus_tag="PC479_000538"
     CDS             40362..41081
                     /locus_tag="PC479_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2803126.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(41248..42252)
                     /locus_tag="PC479_000539"
     CDS             complement(41248..42252)
                     /locus_tag="PC479_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2803127.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            42518..43414
                     /locus_tag="PC479_000540"
     CDS             42518..43414
                     /locus_tag="PC479_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2803128.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            43535..44797
                     /locus_tag="PC479_000541"
     CDS             43535..44797
                     /locus_tag="PC479_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803129.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            44873..46066
                     /locus_tag="PC479_000542"
     CDS             44873..46066
                     /locus_tag="PC479_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2803130.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            46695..47489
                     /locus_tag="PC479_000543"
     CDS             46695..47489
                     /locus_tag="PC479_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2803131.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            47584..48924
                     /locus_tag="PC479_000544"
     CDS             47584..48924
                     /locus_tag="PC479_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2803132.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            49061..50293
                     /locus_tag="PC479_000545"
     CDS             49061..50293
                     /locus_tag="PC479_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2803133.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            50333..51475
                     /locus_tag="PC479_000546"
     CDS             50333..51475
                     /locus_tag="PC479_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2803134.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            51486..52280
                     /locus_tag="PC479_000547"
     CDS             51486..52280
                     /locus_tag="PC479_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2803135.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            52291..53313
                     /locus_tag="PC479_000548"
     CDS             52291..53313
                     /locus_tag="PC479_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2803136.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            53412..54788
                     /locus_tag="PC479_000549"
     CDS             53412..54788
                     /locus_tag="PC479_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2803137.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(55120..55464)
                     /locus_tag="PC479_000550"
     CDS             complement(55120..55464)
                     /locus_tag="PC479_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803138.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            55952..56962
                     /locus_tag="PC479_000551"
     CDS             55952..56962
                     /locus_tag="PC479_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2803139.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            57098..57586
                     /locus_tag="PC479_000552"
     CDS             57098..57586
                     /locus_tag="PC479_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2803140.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            57762..59690
                     /locus_tag="PC479_000553"
     CDS             57762..59690
                     /locus_tag="PC479_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2803141.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            59867..60277
                     /locus_tag="PC479_000554"
     CDS             59867..60277
                     /locus_tag="PC479_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2803142.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            60299..60601
                     /locus_tag="PC479_000555"
     CDS             60299..60601
                     /locus_tag="PC479_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2803143.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(60818..61510)
                     /locus_tag="PC479_000556"
     CDS             complement(60818..61510)
                     /locus_tag="PC479_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2803144.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(61536..62723)
                     /locus_tag="PC479_000557"
     CDS             complement(61536..62723)
                     /locus_tag="PC479_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2803145.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            63059..64936
                     /locus_tag="PC479_000558"
     CDS             63059..64936
                     /locus_tag="PC479_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2803146.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            65293..66699
                     /locus_tag="PC479_000559"
     CDS             65293..66699
                     /locus_tag="PC479_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2803147.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            66727..67575
                     /locus_tag="PC479_000560"
     CDS             66727..67575
                     /locus_tag="PC479_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2803148.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            68236..68928
                     /locus_tag="PC479_000561"
     CDS             68236..68928
                     /locus_tag="PC479_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803149.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            68925..69860
                     /locus_tag="PC479_000562"
     CDS             68925..69860
                     /locus_tag="PC479_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2803150.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            70008..70931
                     /locus_tag="PC479_000563"
     CDS             70008..70931
                     /locus_tag="PC479_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803151.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            70915..71685
                     /locus_tag="PC479_000564"
     CDS             70915..71685
                     /locus_tag="PC479_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803152.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            71695..72477
                     /locus_tag="PC479_000565"
     CDS             71695..72477
                     /locus_tag="PC479_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803153.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            72854..73390
                     /locus_tag="PC479_000566"
     CDS             72854..73390
                     /locus_tag="PC479_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2803154.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            73422..73811
                     /locus_tag="PC479_000567"
     CDS             73422..73811
                     /locus_tag="PC479_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2803155.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            73848..74234
                     /locus_tag="PC479_000568"
     CDS             73848..74234
                     /locus_tag="PC479_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2803156.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            74361..75803
                     /locus_tag="PC479_000569"
     CDS             74361..75803
                     /locus_tag="PC479_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2803157.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            76100..76915
                     /locus_tag="PC479_000570"
     CDS             76100..76915
                     /locus_tag="PC479_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2803158.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT        29097 a         9094 c        13920 g        25216 t
ORIGIN      
        1 attttgtagt gtaatcgtat tttttattaa ttcttacaaa atcaatgaaa ataagtatat
       61 ccattttaaa gtacataaat ttaaaaatcc agaaaaatat acttaaatta tgtaagtaaa
      121 agaggggttg aatcagttga aaatgtaata ttagtaaata gtacatcaat cctatatacc
      181 ctgtaagcaa ctctattttc atgtactaaa aatgtaccta ttttattaac tgaaaagaat
      241 aaattaaatg taagtacata agaagataga aaaaattaaa aacaaaaaat atttatataa
      301 ataaatataa aaaatgatat aatccctatt aaaaaattta tagatgatga agaaatatca
      361 aaatcttata ttataggaag agacacttct ttgccaaaat ctatagaaaa gcaataccaa
      421 atccaaagag aataggcgaa gaaaaaaata aaataaattc aagagtaata gaaaagtttt
      481 atagaataaa gaactgataa ctctttatat aacgaaaaat agaattttaa gagaaaatga
      541 tttaataaat gatttaacat ggtgttttag caggtaaaaa taaatcacca gtaatgatta
      601 tagaaaaatt actttatgaa tctcaaaaaa cttagttaag agtaagagat ttgataagat
      661 aactaaagtt ggggaaatgt gtttgatgag acaaaaaatt agtaaaatag tataatctaa
      721 ataaggatat tttgttgatt aaactacact aatagtaata ttgtaggggg aaaagatgag
      781 agaagaatcg aactctttaa gtttagacca tatagaaatt ttctttgaac ttttatctca
      841 aagtacagaa gattatatat ttttttggga tataaatagg aataaattta aaatttcttc
      901 agcaattttt gatgaattta acttaagtaa agagatagaa tctgatttgg tcaattgttg
      961 gagtaaaatt gtttatccgg atgatgttca aatatggaaa gatgatatac aagaattact
     1021 acatggaaaa aaaggtgagc acaatttaga atataggttg attaataaat ataaagaaat
     1081 agtatggatt tcttgtagag gtaaagttta tgtgtcggat gacccaaaaa ctatatttct
     1141 agtcgggaga ataaagaata ttggtgaaaa aaataaattt gattctatca caggaacgtg
     1201 gaatagagaa caatttgaac atagaatgaa ttatcttata aaagagaaaa tttataaaaa
     1261 tggtgctatg tttattatgg atattgataa ttttaaaaat ataaatgaaa aatatggaca
     1321 ttcttatggt gacaaagtat tgcgtgcaat tgctactgaa gtattagaat atcttccaaa
     1381 agatgtaaga ttgtataggt tagatggaga tgaatttgca tttttttacc ctatgtgtac
     1441 taaagaaact atagagaaaa tatatgaaaa gatacagatg tatacaaata ctcagcatga
     1501 aattgaatca aataagtact attgcacagt gactgcaggc gttgctatgt atccagaaga
     1561 tggagataat tatttagatt tatttaaaca tgctgatatt gcattggata tagcaaaaat
     1621 tagtggtaaa aatagaatta agtttttttc acaagagctc tatgaaaata aactaaaggt
     1681 aatttcaatg caacaaaaac taagagagtg cgttgaaaat aattttaatg attttgagtt
     1741 gttttttcaa ccccaggtaa atgctgttac aaaagaggta ataggggcag aggtactttt
     1801 aagatggcat tcgagtactt atggagaagt atctcctgtt gaatttatac caatactaga
     1861 acagagtaat ttaattatac cagttgggaa gtggattata aaagaagctg taaaacagtg
     1921 taaagaatgg cataaaataa atccagactt taaaatatct gtaaatgtat cgtatattca
     1981 gttaaaggaa gattttttta gagattttat agtagaatgt ttagtagagt atcaattaag
     2041 acctgaattt ttaattttgg aattaacaga gaattgttgg ataccagata taaatttact
     2101 aaatgataaa tttattagtt taaagggtat aggagtatat attgcaatag atgactttgg
     2161 tacaggatat tcatctttaa attaccttaa agaactgtct gttaatataa taaaaattga
     2221 aagaagtttt gttaagaata ttacatataa tagttatgaa tatacttttc ttgaatatat
     2281 tattaaatta gctcatatta taaatttaaa agtttgtgtt gagggaatag agtcttatga
     2341 agagtatgat atagtaaaaa gcttaggtgt agatattatt cagggttttt tatttggaag
     2401 acctgtaagt gcctctgaat tttataaatt tcagctatca aagtgatgta tataaaatga
     2461 gatatatagt atataaaaat agggcaaagt ggaagttttg aagtttatta aaaatttaca
     2521 ctttacccta ttttgttatg tcttctataa aggattaata tactacacta actattatat
     2581 actttcactc caaatttaga gattatttca ctagcttttt ggacatcttc cttagtggca
     2641 tcaggaacat cttttagttc ataatcgata cctaaacttt cccatttgtg tattcccata
     2701 gaatggtatg gtagcaattc aaatctttct acatttttca atgtagctac aaattgacct
     2761 agtttttcta aattatctat atcatcagta atgccaggaa ctaaaacatg tcttatccat
     2821 acaggaatat ttcttgattc taagtgtcta gcaagtttta atgccttatc cataccaaca
     2881 ccagtcaaat caatagattt ttcctcaacc atatgtttta aatcaagtaa aaccaaatca
     2941 atattatcta aaactgggtc tatagtttct atatctacaa atccagatgt atccaagcaa
     3001 gtgtgtatat cattttcttt tgctttttta aataattcac ttaaaaattc tggttgaaga
     3061 gtggattcac caccagaggc agttatacct ccaccagaaa acttcataaa agaagtatac
     3121 ttaagagcat ccgttattat ttcatcaact gtgtattctt ttcctgattt aatatcccat
     3181 gtatccctgt tatgacagta tttgcatcta agaggacacc cctgaaaaaa taatatatat
     3241 cttattcctg gtccatctac agtaccaaac gtttctatag aatgtatctt tcccttaatc
     3301 atagagttca cctcacaata tattttaata accttatcaa tagttataat atttctctaa
     3361 aaaaggccat cttataccag aaataattca aatataagat agccttttat ttttattttt
     3421 tacacagtag taatcttagt taaagtataa aaatttaaag actaagccat tttaccgtgg
     3481 aatgttctgt ttataacatc taattgttgc tctttagtta attttatgaa gttaactgca
     3541 tatccagata ctcttatagt taattgtgga tattcttctg gatgttccat agcatcttct
     3601 aaagtagctc tatcaaatac atttacattt aagtgatgag cattttgagc gaagtatcca
     3661 tccatcattg cagaaagatt gtttattctt tcagtcatat cttttcctaa agcaccaggt
     3721 actatagaga aagtattaga tataccatct tgagcatgtt catatggtaa tttagcaact
     3781 gaagataatg aagctaaagc accattagta tctctaccat gcattgggtt agcaccagga
     3841 gcaaatggag caccagctct tctaccatct ggtgtattac cagttttctt accataaaca
     3901 acatttgaag ttattgtaag tatagattga gttggataag aatttctata agttttattc
     3961 tttcttattt tattcataaa gctttcaact aactctacag ctatactatc aactctatca
     4021 tcattatttc cgtattttgg ataatctcct tctatttcaa aatcaacagc tattccttgt
     4081 tcatttctaa tagttttaac cttagcatgt tttatagcag ataatgagtc tgcacaaact
     4141 gataacccag ccataccaca agccatagtt ctaaatatat ctctatcatg taatgccatt
     4201 tctaatgctt cataagaata tttatcatgc atataatgta ttacatttaa agtatttaca
     4261 tataatgttg ctaaccaatc agtgaatggt tcaaatttac tcataacttc atcataatct
     4321 aagtactcag aagttatagg ttcaaatctt ggtccaactt gaacaccaga tttctcatct
     4381 acaccaccat ttatagcgta taataaagtt ttagctaagt taactctagc tccaaagaac
     4441 tgcatttgtt taccaattct cattgcagat acacaacaag ctataccata gtcatctccc
     4501 cagtatgctc tcattaaatc atcattttca tactgtactg aacttgtatc tatagatact
     4561 ttagaacaga aatctttgaa tccttgaggt aatttagttg accatagaac tgttaagttt
     4621 ggttctggag aaggtcctaa tgtatataga gtatttaaca ttctaaatga gtttttagta
     4681 actaatgttc taccatctat tcccatacct cctatacact cagttaccca agttgggtct
     4741 ccagagaata ggttattgta atcaggagtt cttaggaact taaccattct taatttcata
     4801 acaaaatggt ccatatactc ttgtaattct tcttctgtta cgattccagc ttttaaatct
     4861 ctttcaaagt atatatctaa gaaagtagat gttctaccta aagacattgc agcaccattt
     4921 tggtctttaa cagcacctaa ataaccaaag tataaccatt gtacagcttc ttttgagtta
     4981 gttgctggtt ttgatatatc aaatccataa gattcagcca tttttttaag ttcatttaaa
     5041 gctgatattt gctcagttat ttcttctctt aatctgataa cttcctcatc catgcaagaa
     5101 acttctaatg atttcttttg ctcattttta tcttctatta aagcatcaac accgtataga
     5161 gcaactcttc tgtagtcacc aattattcta cctctaccat aagcatctgg aagacctgtt
     5221 attataccag attttctagc agctctcatt tctggagtat aaacatcaaa aactccttgg
     5281 ttgtgtgttt ttctgtattt agtaaatata tcttttattt ctgggtctaa ttcatatcca
     5341 aaagcttcac atgaattttc aaccatcttt ataccaccat atggcatgac agctcttttt
     5401 aaaggggcat ccgtttgtaa cccaactatt gtttctaatt ctttatctaa atatcctgga
     5461 gcatatgcag ctattccaga aacagttttt gtatcaacat ctaaagtacc accattttct
     5521 ctttcttttt taaataggac cattgcttcg tcccataatt ttttagtatt ttcagtggca
     5581 ccagctaaga aagagtcatt tccctcatat ggagaatagt ttaattgtat aaactctcta
     5641 acatttattt ctttagtcca tcttcctgtt ttaaatcctt gccatgcatt cattaagtat
     5701 ttcctcctta gatttaaatg ataaataata ctattaaaat taatttttat atacttaatt
     5761 tattttatat atttttttaa aaatatattt tctaatataa taatagcata ttattcgtat
     5821 cctgtataca taaaaaataa aaaattgtcg ctttaaagta taaaacagat tatatgttaa
     5881 taatgtggtg aaattatacc catatataaa taaaataaaa caaataaata gctaattaag
     5941 gagaaaatat tttttatttt ttgtttagga taaataaatt ttattatgga aaaaagaaaa
     6001 agcatacatc ttcacaaact gaatatttat ttaatagggg gaggtgatag attgtttatc
     6061 acagtggtat tatttttagt aggtttccta ttaattacta aaggcgcaga tatctttata
     6121 aattgtactg tagagatagg taaaaaaacg aatatatcag aaattatact tggagctact
     6181 atagttagtt ttgcaacaac attacctgag tttacagtgt ctctacttgc ttctattgat
     6241 ggtcatacta ctatgagttt aggaaatgca gttggttcta taatatgtaa tacagggtta
     6301 gcattagggt tggttgtttt tataagtcca tttaatgtag ataaaaaaat gtttttttct
     6361 aagtctttac tattaatagt atccgttata gttttaattt tattgagttt agatggagtt
     6421 ataacaagag gtgattcatt attacttata ataatattaa tcttttatat ggttaataat
     6481 tatagaagtg tcgttggaaa atctgatact aaaaatagaa atattaaaaa taacactatt
     6541 aaagacatat ctactaaaaa agggaaaaat tatagaggtt tttccgtatt agaaattgta
     6601 aaaatactat tactttttgc tacaggattg ataatgatga taataggttc acaaatcctt
     6661 atagagagtg gagtaataat agctagtttt ttaaatatac ctcaaggtat agtaagttta
     6721 actataatag cacttggaac ttctctgcca gagatagttt cgtcgattac tgctataaga
     6781 aaaaatcatc atgaaatatc agtaggaaat attttgggtg caaacatatt gaatatagta
     6841 tcagtaatag ctgtatcagc aataccaaat aacataccaa tactatctca aaatagacaa
     6901 ttggatatac cattcatgat attattatta ttaatagtaa taataccaac acttaaatct
     6961 aataaactta gtagaataca aggtatattg atgttattca catattttct gtacatatca
     7021 attttgtatt tcatgtatat tatttaacta tactgtttaa aatattaatt gcagaatatc
     7081 ctaaaaaagt atataatgga taggttaaga ttaattgata acggagagga ataatatatg
     7141 aatataacaa agtttgaaga tttaccaata agtgagggaa taaaaaaagc tatagctgaa
     7201 atggggtttg aagaaccatc tcctatacaa gcacagtcta tacctgcgat tttgtcaggt
     7261 aaagacgtta taggacaagc tcaaactggt acgggaaaaa cagctgcttt tagtatccct
     7321 atactagaaa caatagaccc aaataataga agtttacaag ctgtagtgct ttgtccaaca
     7381 agagaactgg caatacaagt atcaacagaa ataagaaagt tagctaagta ttcgcacgga
     7441 ataaagacat tacctatata tggtggtcag ccaatagata gacaaataaa atctctaaag
     7501 agtggtgtgc aagttgtaat aggtacacca ggacgtacaa tagaccatat caatcgtaaa
     7561 acattaaaga tggataatgt aaaaatgatt atattagatg aagcagatga aatgctagat
     7621 atgggattta gagaagatat agaaatgatt ttaagtaaga ttccagaaga aagacaaaca
     7681 actttctttt cagctactat gccaagagga attttggaat taactaagag atatcaaaaa
     7741 gacccagagc atataaaagt tgttagaaaa gaacttacag tttcaaatac aaagcaatac
     7801 tatatagaaa ctaggtcatc aaataaatta gaagtattat gtagattagt agatgtatat
     7861 gaccctaaac tatctgttgt tttctgtaat actaagagaa aagcagacga attggtagga
     7921 gatttacaag caagaggata ttttgcagat gcattacatg gtgacttaaa acagactcaa
     7981 agagatatag taatggataa gtttagaaat ggtacaatag atatacttgt tgctactgat
     8041 gttgcagcta gaggtataga tgttgatgat gttgaatgtg tattcaacta tgacttacca
     8101 caagatgaag aatattacgt tcatagaatt ggtagaactg gtcgtgctgg tagagaaggt
     8161 atgtcattta cttttgtatt tggaaaagaa atgagaaaga tgaaagatat agaaagatat
     8221 actaaatcta aactaataaa gcataatata ccaacaataa ctgatgttga agagaaaaaa
     8281 gtaggtactt tctttgcaca agttaaacaa actatagaag aaggtcactt aactaaacaa
     8341 ttacaatggt tagaaggttt ctgtaatgat gaagattatg caatggttga tatagctgct
     8401 gcacttgtaa aactatctct aggtgaagaa atgaaagaag aaataataga agaaaaacct
     8461 agaagagagc gtggagatag aaaaggtggt actggagcta aagatggtat gattagatta
     8521 tttatcaata ttggtagaaa tcaaagagta caagcaaaag atatagttgg tgccatagct
     8581 ggagaagttg gaataccagg taaagtagta ggtactatag atatatatga taagtatact
     8641 tttgttgaaa tacctaaaaa agatgcaaaa acagtaattg aaaagatgaa agatattaag
     8701 ataaaaggta ataagattaa tatagaaaaa gcaaataaga agaaaaaata acgtatagta
     8761 tacaaaacta taattagaaa atatataaaa taatatattg gatatttaaa aagcctgatg
     8821 tgttttcaaa acagatagta gataatctgt aagagttaac agcataaggc ttttcttgtt
     8881 ttatatgatg ttataatttt ccagattgtt gtgaaaaatg tatattagtt cctttttttc
     8941 aaatctgatt tgaaaaagag aatattaatt atctatatag tttagattat aataaaaaat
     9001 gtaagataac aaaggggaga aaaagatgtc aatgacttat atggacccta gggttttttt
     9061 gattttagaa atggtgttag aagaagacac aaatactcta gaacaatttt caaatgtttt
     9121 gggtgttagt actaggacta tcagaaacta tataaaccag attaatcgag aattaaaagg
     9181 tggtattgct aaaatagtta aaaatgaaaa aggtacatat agtataagaa ttgaggatga
     9241 aagtaaatta attgagattg taaattttaa tagaggaaag ctatcaaatt ttataaattt
     9301 aaattcacct gatgaaagaa taaattatgt tcttgatatt ttaactatga ccaacaatcc
     9361 aattaccata gatgatttgg ctgaagagat atgtatagga agaacaacat taataaagga
     9421 tttaaaaaaa gcagacagaa ttttagctga atatgattta gaattaaaaa ggaagccaaa
     9481 tataggtatg attttagttg gtgaagaaat ggatataaga ttattaatcc ttgatagatt
     9541 gtatgaaaat tatattgatg tcttggaaaa cgttaatcag ataaatttag ttaaagatta
     9601 tgatattgaa tgtcttagag atgaattaaa aaagttattt aaaaaagaag aactctacat
     9661 aacagaacag gtcttgagag atgttgaaag atatataatc atgtctgtct taagaaatct
     9721 aaatgggtac aaatttgaaa agatagataa gagatttgaa gtgataagat gttcagatga
     9781 atatacttta ggattaaagc tcaaagcttt attggaaaag atatttaata tagttttaaa
     9841 tgaaaaagaa accatatttt tgacaatgcc attaataggt agaaaagctc ctagtacaaa
     9901 attagcactt agcagtataa agataaatga tagcgtaaag gaagttgtag atgaagtaac
     9961 tagtttttta ttagagacct cagggattga ttttaaagaa gataaaaagc ttatagaaaa
    10021 tttggaatat cacttgtatt ttgcacttaa tagaatgaga ttcaacataa gggtaaaaaa
    10081 tcctctttta caagaaataa aggaaagata tacatttcca tatagtgttg caaagatagc
    10141 tgcaatgata attgaggaaa agtttgactt gaaaatatgt gaccacgaaa taggatatat
    10201 agcattacat tttggaagtt attttgaaaa aaattcaaga aaagttctaa atgtaaatag
    10261 ggtagctgtt gtttgtggaa caggtcttgg gactgcacaa cttattagaa taaagatagg
    10321 aaaaatactt ggagaaagtg tagaagtaaa tactttttct gatatagaag ctaaagctaa
    10381 tttagattta ttaaagtatt atgatttagt atttacaaca gtagatttca aaacagactt
    10441 agatgttcca atttttagag taaatgcgtt atttaatgaa gaaagtataa agaaagagat
    10501 agaaaatgca atacttttga aaaatagtat aaatcttaag tctataaaaa agattgaaaa
    10561 aaatatccct tttataggta aattaattga agaggataag ttctttatat tgaataagag
    10621 tacgtttatg gaaaatttag aagatatgtt agaaaaatta gttcataaag gaattattga
    10681 tatagagttc aaaaaaaagg tattaaaaag agaaaagaaa tcttcaacag cattagataa
    10741 ttatatagca ttgcctcact cagttaatac taatggggaa aatctttatc tggcatttgg
    10801 aatattagaa aaaccagtga tttgggatac aaaggaaatt agaataataa tattgatgat
    10861 aattccagat aagaatgtag atagtacaga cctgataata aaaagttatg aagaagtact
    10921 tgaagtaggg agagataaaa agatggtaga agaacttgct aaggcaaaat catttgatga
    10981 attttataaa ataattacca agaggaggta ataatatgga cccagtaatt tttattatag
    11041 catcacttat tacagtgtat ttattaaata tttttcttgg atacttacag cttaaagatt
    11101 ttaataaaaa ttatattgaa ctaaaaagaa aaggaagagt ggcaattggc agaaaaaaag
    11161 gaagaatttc atcgggtaca attgtgttga tacttataaa tgatgatggt gtgattgtcg
    11221 agactagaaa aatgcaaggt gttacagtat tggcaagagt aaagtcattt gaaggtttgg
    11281 taggaaagaa cttagagtat atagaaaaat cagatttaac tgtttacaat aaactactta
    11341 aaaaagcaat actagatgca gtcaaacagt acataacatt tgagaaaaat aaatgtgaag
    11401 atacaaataa tctgagtact gatgaagagt gtaggatgga agtagtttaa aaaaatatat
    11461 ttataaggag gagttaatat gacgcaattt tttcaagtat tagcaagtgg tgcagatagt
    11521 tttatgaatt tatttagggc aggtgggcaa caatttgtag gttttgtaac agatatagta
    11581 ccacttttag tagcattatt agttacaatg aatgcaatta tcaactttat tggtacagat
    11641 agagtcgaga gacttgcaaa aaaatgttct gcaaatatat ttactagata tttgatatta
    11701 ccagtccttg gtacatttgt atttgcaaac ccaatgacac tatcattagg aagattcttg
    11761 ccagaaaagt ttaaacctag ttactttgca gcagcaagct tttcatgcca tacaatgaat
    11821 ggattattcc cacatatcaa tccaggtgaa ctatttatat atctaggaat tgcaaatggt
    11881 ataacaactt tgggattctc aacagcagat ttagcagtta gatatttgtt agtgggtata
    11941 gttgcaaact ttattaaagg tgtagttact gactatacaa caaaatttgt agagaaacaa
    12001 caaggaatag cattaaattc agatataaag attggataat tactttaagg tataaataaa
    12061 tttttcaaat aaaaagatag tgtatagagg aggataaatt atatggcatt taacaaagtt
    12121 aaaatagtaa aaggttctgg aggatggggt ggaccattga taatagaacc aacagaaaag
    12181 aagaataagg tagtatatgt gactggggga gcaaagccag aaactgcagt aaaaatagct
    12241 gaacttacag gatgtgagtt aatagatgga ttttcacagg gtgttaaaga tgatgaaatc
    12301 gcatgtgtaa taataaattg tggtggaaca ttgagatgtg gtatatatcc tcaaaagaaa
    12361 ataccgacta taaatataat gaaaacagga aaaagtggac cattagcaat gtttataaaa
    12421 gaagatatat atgtttcagg ggtaaaacca acaaatgtag agcagttata attgaggtag
    12481 taggagggaa gaaatatggc ttatgatact aaaaagaaga taagtgagca gagtcaagga
    12541 attgttgcaa aaataggtat agcactagga aatgtgacaa gtactctttt tcaagctggt
    12601 agggatacta tagatacatt aatacatact atactgcctt ttatggcttt cgttgcagtc
    12661 ctaataggta taataaatca atcaggtata ggaaattggg tagccaattt acttactcca
    12721 ttaatcggaa atgttggagg tttagttgtc ttatcaataa tatgttcatt tccgttgttg
    12781 tctccgtttc ttggaccagg agctgttata gctcagatag tgggagtatt ggttggtaca
    12841 gaaataggaa aaggaaatat tccaccaagt ttagcattac cagcattgtt tgcaataaac
    12901 tcacaagcag cttgtgactt tataccagta ggtcttggtc ttgcagaagc tgaacctgat
    12961 acagttcaag taggagttcc gtcagtttta tattctagat ttttaacagg tgcaccaact
    13021 gttctattag catggttagc aagctttggg ttatataaat agaagaatac tttataaata
    13081 attagagaga ggttcagttg atatggaatt aatatacagt actcaaataa ataaaatagg
    13141 agataatgca aatgaatttt tacaacataa tatgtttgta actttcaaag ataatgctcc
    13201 tcaagagtta gaaaattact gttatataca ttctgaaaat aatttagtaa aagaaatact
    13261 tacggatgat gtgctatcaa taaataatgt tgattacaaa attacttctg ttggtgagtt
    13321 agtaaaccaa aatctttcag aattaggtca tataacattt aagtttacag gtgaaaaaga
    13381 tggagcaatt ggaggaacct tatatcttga agaaaaagaa atagcaccta ttggcgtagg
    13441 tactatacta aaaataatta gaaaataaaa tttaaaacga atctaatcat attataagta
    13501 aattatagat aaaagtcaat ttaactaatc aagttaactt ttaatgtgat aaaaaagggg
    13561 aaatgaaaat gagaaaagtg gctttagtcg ttggaggagg aagaagttta ggtgaatatt
    13621 tatctaaaca tttagctaaa gtaggttatg atgttgctat tgcagattta aatgaagaca
    13681 acgcaaaaaa agtagctgaa gaaataaaaa ctgaatttgg ttgtaaatct gtaggatata
    13741 gttgtaatgc catagatgaa agacaggtta taagtactgt caaagctatt gaaaatgatt
    13801 ttgatagaat agatttatta gtgtacaatg caggagtggc tgtaagtaaa aaaataactg
    13861 attttggata caaagatttt aaatttattg tagacgtaaa tttaaatggg tactttctat
    13921 ttgcaagaga agtatctaaa gtcatgatag caaaaggtat tgagggtaga ataatacaga
    13981 taaattcaaa atcaggtaga gtgggaagca agcataattc aggatattca gcaggtaaat
    14041 ttgctggagt aggacttact caaagtttag cgctagattt ggcagaacat aagataactg
    14101 taaatgcact gatgctcggt aatttacttg attctgatat gtttgaaagt ttaattcctc
    14161 aatatgcagt aaaattggga atacctgaat cagaagtcaa gcaagtttat atagataaag
    14221 tacctttaaa aagagggtgt agatttatag atgttgcaaa tatgcttaca ttttatgctt
    14281 caaaggaagc tgaatactgc actggtcaat ctataaatat tactggtgga caagttatgt
    14341 aaggttttta tggagtgtct caagatgaac tctttagttc atgaggcact tttttgtata
    14401 aaattaaata tattttcagc atttaaacaa tgaaaaagag gtttatcttt attttgagat
    14461 agcctcattt ttgtaattaa aaaacatagt gtttaagtaa acatataaga ttgttagttt
    14521 tttaaaagag aattttttaa taatattata tttatgaagt atttttaaac ttattttgat
    14581 aaaatatagc tatgttttat atactgtagt tttgaacata gaaagtttaa taaattatag
    14641 tatatatgtt aatttttaat aaggtaagaa agtagaaaga aggatatttt atggatataa
    14701 taaaaaaatt aagtcatggg tgtacattag attgtcatga ctgttgtaaa ttcaatgttt
    14761 atactaaagg aaataatgta gtaaaaattg aaggagataa aaatcatcct tatacaaaag
    14821 gttttatatg caaaaaaggg atggcccatt tagatagatt aaatcataaa gatagaataa
    14881 agactcctat gcttaaagtt gatggagtat ggaaagagat ttcttttgat aaagctatag
    14941 aaataatggc agaaaagctt acatactata aagaaaagta tacatcaaag tctgtcatgc
    15001 attatgacca atatggaagt gggtctgtgc taaaatacat aggagatata ttttttaatt
    15061 tttatggagg tgttagcaga cataaaggtg gtccctgttg gagtgcaggg atgcatgctc
    15121 aaaaatatga ttttggagta gctaagagtc atgctataga agatatgtta aatagtaaaa
    15181 gtatatttgt gtggggaaaa aatccagcat atactacaat tcatactatg caaataatta
    15241 aaaaagctaa agaaaaaggg ataaagatag tagtgattga ccccatatat acaaaaacag
    15301 ctcaaatagc agataagtat gtacaagtaa atcctggaac agatggagct ttagctatag
    15361 ctatggcaaa aattatagta gaagataaat tatatgatga agaatatata aactcgtatg
    15421 ttattggttt tgaagaatat aaaaaatatt tatcttcatt ggaattaagt tttttaattg
    15481 atgaatgtgg tgttaaagag aatgatataa gagagttagt ggatttatat actaacaaat
    15541 attcaagtat aaatgttgga tatgggcttc aaaaatataa aaatggtgga aacacaataa
    15601 gagcaataga tgctttaggt gctataactg gtcaaatagg atttagtggt ggaggggtaa
    15661 actatgctaa taaagtttat ccgagtgtta taaattctga cccttacaat agccaatcat
    15721 atggtgaaga tagggagttt tatgtaagta atatcagtaa atttatagaa gaatctttaa
    15781 aaaatacatc aaataaagta aattatgcat cagatgaact tgatatgaca tcaaataaag
    15841 taaattatgt atcagatgaa cttgatataa catcaaataa aacagattat atatcaaatg
    15901 aattgtataa tttatctaat aagtcaatca aaggtaatat tcctataaag atggctgtaa
    15961 taactaaaag taatatgctg aatcaactgc cagatttagt agaattggag agggtgtttt
    16021 caaaaattga atttaaagta tgttttgata tgtttataac tgatacagct actttatgtg
    16081 acatgtttat accgtgtaca aatacactag agagtgaaga tataatttat agctcaatga
    16141 ccaatccata tataacctat aatgaaagag ctgtaaagcc agcacataaa ttgatggatg
    16201 aatattactt ctttatggag ctagctaaaa agatgggatt aaatgattat ccttttgtag
    16261 aaaagagaac ttatttagaa aaagtgattg aaccacttaa gcgttttgac aaaaatctgg
    16321 atatagaaaa acttaaaaac aattacttta ctatacataa ccctgttgct tgggaagata
    16381 agaagtttga gactccatct ggaaaatacg agctttattc agaaagtata aaaaacttgg
    16441 gaataagtcc aacacctgta tatataagca ataaatataa agaaatagaa gataaaaata
    16501 tttcttttag attattaact aatcatcatg cagatacttt atttagtcag cattttatgg
    16561 acaaaaaatc tatagctcaa gcatatataa atcggagaat ggctaaaaaa gtaggtattg
    16621 aggacaaaga tattgtgata ttgaggtcaa aaaaagcaaa aataaatgta caaataaata
    16681 tagatgatgg agtaggtaat tatatagtaa aaatgtatgt tggttggtgg aagaaacatg
    16741 gaaatcctaa tagtttaaca gacactggta tatcagattt tgggggtcag gtaacatata
    16801 atgaaagtat ggtagaaata atacgacaaa actagttaaa tttcattgaa ggaataaaaa
    16861 tataattata gaattgatta aaagttacta tttttacaaa actattgaag gagggatttt
    16921 tgatggtaaa ttattcctta gaacataaga atctatacat agaatttatg tgctcagaac
    16981 tagaccacca tgtagccaat gaaataaggg aagaaataga taacttatta agtgtaaatc
    17041 aagtaaaaaa tgttgtattt aattttgaaa atattaattt tatggattct tcaggtatag
    17101 gcgtcatcat aggtagatat aaaaagattt ctaatgaagg gggacgagtt tcagtaatta
    17161 atataagttc acgagttaag aagatttttg atttatctgg gttaaataaa attattggca
    17221 tttatgatac gtatgaagaa gctctaagtt ctttgtaggg aggagtatgt gatgaataat
    17281 attatggaag ttaaattttc ggcaatatct gaaaatgaaa gtttagcaag agttatagta
    17341 gcatcctttg cagctaagtt agacccaaca gtagatgagt tagtagatat aaaaactgct
    17401 gtctctgagg ctgttacgaa tgctataata catggatatg aagaagacag ttctaagttt
    17461 gttttcttaa gatgtgagat agaaggaaat actataaaga ttatagttga agatgaaggg
    17521 tatggaatag aaaatgtgga gaaggcaatg gaaccacttt atacatcaaa accagaactt
    17581 gatagatcag gaatgggatt cacagtaatg aaaagcttta tggacgatgt agaggtaagt
    17641 tctgtaaaag ataatgggac tagaattgaa atgactaaaa aaataaatgt gcctaaataa
    17701 agtccaaaag tgaggtttta ttatggaagt aactgttgcc agagaagaaa aaaaacctct
    17761 tcttagtcat gaagagactc tagaacttat tgaaaaagtt caaaatggag atgaagaggc
    17821 aaaagaaata ttaatatcaa gcaatttagg tctagttagg agcgttgtat ctagattttt
    17881 aaatatagga tatgatagag aagatttatt tcagttaggt tctataggct taataaaatc
    17941 aatatataaa tttgacccaa aatttaatgt aaaattctct acttatgcag taccaatgat
    18001 tttaggtgaa ataaagagat acttaagaga tgatggtatg ataaaagtat caaggtcgtt
    18061 aaagcaaata gctataaagg caaagatgga aagtgaagct ttaactaaaa aattaggtag
    18121 agagccgagt atagaagaat tagctaaagc aatagatgta gaaaaagaag atttagtaat
    18181 ggctatggaa gctaatttca atgttgagta tttgcaagga gttattcatg aagaggaagg
    18241 ttctccaata tgccttattg ataaaataag catgaaagga gaaagtgaag aagaaaaagt
    18301 agtggataac atattgttaa aagacatact tggaagactg gataagagag agcgtcaaat
    18361 aatagttctc agatactttg aagacatgac tcaaagtgaa ataggagaga tgctcaatat
    18421 atcacaagtt caagtatcta ggatagagaa gaaagtattg tctaagttaa aagaatatat
    18481 atcgtgataa tacttatgat atgtagaata ataaaatata ataaatatat tactaaaata
    18541 aaatgtaata actttttatg ttaagttttt aaattattaa aagatattaa aataataaaa
    18601 agcattaaaa aagtaaaaat ataaaaacaa ggtgtcttta tggaaaaata gattttttct
    18661 ttattaggca tcttttttat tttttgacca aactttagat agtcaaaata aataataggt
    18721 ttattttttt aaaattaaca aataatactt tagagaggtg tttagtatgg ataaaaatta
    18781 taaaaaatat gtagaccaaa taagcccaaa acctacatat ttaaaaaact atacacttgc
    18841 atttattgta ggtggaatta tttgtatgat agggcaagca ataaatgatt tgtatatgaa
    18901 agttggaggt cttgataaac taggtgctag ttctgcaaca tcaataacat taatatttat
    18961 aggggcattt ttaactggat taggagttta cgacctaata ggaaaaagag ctggagctgg
    19021 ttctataatt ccaataacag gttttgctaa ctcaatagta tctccagcaa tggagtataa
    19081 aagagaaggc tatgtattag gtgtaggtgc aaacttattt aaaattgcag gaccagtttt
    19141 agtttatggg ataggttctt ctattttatg tggaattatt tattatattt ttaagatgtt
    19201 ttagatgatt tttatttaat ttaaatttta ttaattatga agaaggtata aaatctgaaa
    19261 atagctacta aagtaactaa ttgaaaaata attctgcaga tttaattaat atttttaatt
    19321 aatattaaaa atttgaatag taaaatgcaa aattaaaaag gtgtgaaaat atgaaaaata
    19381 aaagaattgg aaaaagaaca gtcaagttag aaaataaacc aaccataata tcaactggaa
    19441 ctatagtagg tcctaaggaa ggtgaaggac cactaaagga ctattttgat atgattatga
    19501 ctgacgattt atatggtgaa aagacttggg aattagctga aagtaaaatg gtagagactg
    19561 catctcaaca agctatccaa aaggcaggta aaaaattgtc agatgtaaat tatatgctag
    19621 gtggcgattt aataaatcaa atagttccag catcctttgc agcaagagaa ctggctattc
    19681 catttttagg tatatatgga gcttgttcta caatggcaga aggtctttgt ataggttcta
    19741 tgcttataga tggtggtttt gctgatttag tactgtcagg tacatcaagt cactattgta
    19801 cagcagaaag acaatttaga ttcccacttg aacttggaaa tcaaaaacca atgacagccc
    19861 agtggacagt tacaggagca ggaagtgtat tattagctcc taatggagac ggtccaaaag
    19921 taaagtatgt tactgttggt aaagtaatag atgagggtat tgatgatggt aataatatgg
    19981 gaccagctat ggctccagca gcaatagata ctatctactc ttactttgaa gatactaatg
    20041 atgacccaaa tagctttgat ataattgcta ctggagattt aggtactctt ggaaaacaga
    20101 tagcagaaga ctttctaaaa gaaaaaggtg tagatatatc aaaagtttat actgactgtg
    20161 gtatagagat gtttaacctt aaagaacaag atgtccactg tggtggaagt ggatgtgggt
    20221 gttcagctac agtgtttgca ggatatatat atgataagtt gagaaaaaaa gaatttaaca
    20281 aagtaatgct tgtatcaaca ggagcactac tttcaccgac aagtacacta caaaagcaga
    20341 caataccatg tgttgcacat gcagtagtaa tagtaaatga gtaggaagtg atataatatg
    20401 ttaatggact atgtaagagt atttattgta ggtggaatta tatgtttaat agcccaagta
    20461 atgatggatt atttcaaaat gcaaactcca tatgttatgg taacgtatgt aacaactggt
    20521 gtagttttaa cttttctagg actttatgag ccattagtta agtttgcagc agcaggtgca
    20581 acagttccta taataggatt tggatattca cttgctacag gagtaataaa atcaattcaa
    20641 agtgatggtt ttttaggtat ttttttagga ggtacaactg ctacagctgg tggaatagca
    20701 gcagctatat tctttggata tattatgtca gtagtattta ctccaaaagc aaaaccataa
    20761 atctttatta aaagaataat aagtttttga aataataact ttttataaag caggtggata
    20821 tcttttagat tccacctgtt ttttatataa agaagcgttt acataattga agttgacaat
    20881 tatttacaac atagtgtagt attatattat caaaataaaa tgaagtgaag taggggataa
    20941 agatggtaac tattaaagat attgcaagga atctaggtat cagttattca actgtttcta
    21001 ggtgcttaaa caataatcct aatgtatctg aaaaaacaaa aaagaaagtg gtagaagagg
    21061 caaatagact agggttccat tttaatgtaa atgcaagaaa tttagcaaaa aaagaaacta
    21121 acaggatagg ggtaatcttt tcaaataatt ttaatcatca agatactaga aagtttttta
    21181 gtgatataat ggatagttct ataaattcta ttgagactaa taaatacgat tttataatac
    21241 agcctaataa taacattagt ggtgatagta atgtatataa aatggtaaat ggacaaatgg
    21301 ttgacggatt agtaattgtg tctcaatcaa taaaaaaaga agagtatgat tttttaaaag
    21361 acaataattt tccacatgta tttatatatt ttaaaccatc ttttatagga gaagtagata
    21421 atttcttttg ggacgataac gtgtatggag gttatatagc taccaagcat ttaattgacc
    21481 atggtcataa agatataata acagtaactt cagatgataa gagttcaaaa atgcatgaag
    21541 atagaaccaa gggatatttg aatgctatgg aagaagcaaa tttaaagaca gaagtcatta
    21601 aatctaaaat ggattttgaa tctcaagttg aatttttaga aaaatacatt gataaaataa
    21661 aaaaagcttc agctatattt gtacaacaag atgtaccagc gatttctata atacaagagt
    21721 taaaaactac ttatggtata aatgtaccag aagatatttc cataataggg tataataata
    21781 ttgaattaat atcatatttt aggtcgcatt tatcaacaat agatgaccca agagagcaag
    21841 ttataaaaaa tggagtagat agtttagtaa atataataaa taaaaagagt acagaacaca
    21901 ttccaagaaa attatatcca aggttaataa tacgaaatag cgttaaaaga ataaagtagt
    21961 aagcttttaa gtatcaaacg tttattaaaa atattataga ttatgtgaag ttttttaaca
    22021 aataatataa ataaaaatta aaaggagtag gtgtatataa gtacattgta ctcctttatt
    22081 tattgagata gtttttataa tatgaatgaa taattttatt caattatttg aaaatccgtt
    22141 attttaacaa tataaattaa taaattattt gtattttata tcatgaatct ataatatttt
    22201 tttgattcat aactaaaagt atataaaaat gatgaataaa tatatattat acattatcaa
    22261 tgataggtta tataataaaa tcactaacaa ttatattata agaaaattat aaaaattaca
    22321 aggaggtata gaaatgaaag aaaaagcagg gattagaaat gccataatag cagcctttat
    22381 aatcacaata ttggcagaat taattggacc tatttcattt aaagttatgg gaattaatgt
    22441 aactttactt agtatactat gggctatatt cataggaatg gctgtttctc ctcatttatt
    22501 agggagagtt ataccagcct taaaaaagtt tataggggat aatgaaataa atgtatctcc
    22561 atatttatta tctttgacac tatatccatt agggataatg tttggaatta atgcagggcc
    22621 taaagttggt atattacttc aagcaggacc agcactttta ttacaagaat ttggtcatat
    22681 gggtacaatg ttgatagcat tacctgttgg agtagcatta ggtcttggaa gaagtgcatt
    22741 aggtggtact ttttcattat gtcgtgacac agcactagga attattggag ataagtatgg
    22801 attaaactca ccagaaggta taggaacact aggaacttac ataagtggta gtatatttgg
    22861 aacattatta ttttcatttt tagcaccagt gggtgtactt ttaggatttc atccatatgc
    22921 tttagcaatg gcatcaggaa tgggtagtgg ttcaatgatg ggagctgcaa cagcatcatt
    22981 aatgaataca gttccacaaa tgtcagagca gattttagca tattcagcta ctagtggatt
    23041 gattacagct gttactggaa tatatataga attattctta tctttaccag tagccaacta
    23101 ctattatagc aaagtagctc cagttattga aagatttaga ggaagaaaaa caagaagaac
    23161 aagtaataaa ttgtaaagga ggatatatat atggaaaaaa cttttaagac tattatggag
    23221 cagataaaaa tagtattgtt tatatatatt attatttgct ttggacaaat gatagcactt
    23281 aagactcagg ctcaagattt gttgattgga agtgtatctg gatttttgtt ggtgataatt
    23341 gctattataa taaagaattt tgttaaaaaa cctaatttac caggttttgc atgggctaca
    23401 ttagttgcat ttgttttatc gcttccaata tctccagtgg gggatatcat agcaacaaat
    23461 gtagggaaaa tcaattttat gtctacagta acaccattat tagcttttgc aggaatctct
    23521 gtaggtaatc aattagatat attaaagact ttgagttgga agttagtagt tatatcattt
    23581 atagttatga catctactta ctttggttct gcttttatat ctcaaacagt tttaagatta
    23641 aatggaatgc tttaaggagg tagtaaatat gaatgaatta aaagcaaaaa ttaaagagga
    23701 aataaagtta ttatctgaag agaaaaagac atcatttgaa aaagtttctg actatttatt
    23761 ttcaaatcca gaattagcat ttgaggagta taagtcacaa aaagcattat gtgatttgtt
    23821 agaagaaaat gaatttaatg taactaaagg tgtaggagga ttagaaacat cttttgaagc
    23881 tgtgtattca aatggaacta atgggaaaac agttgctttt ttagctgaat atgatgcact
    23941 tccaggaatg ggacatgcat gtgggcacaa tatcataggg acaagttctg ttggtgcagg
    24001 tatcatatta aaagaaatca tgaaaaaaca taatatagaa gggactgtaa aggtttttgg
    24061 aacacctgca gaagaaagag taggtggaaa aataactatg attaaagaag gtgtatttaa
    24121 taatgtagat gctgcattaa ttttacatcc aagtgatgct tcaatgcctg atgatatatc
    24181 atttgctcaa gttaatttaa aatttgattt tactggtaag gcatcacatg ctgctgcttt
    24241 tccttgggaa gggaaaagtg ccttaagtgg agtaatagca ctatttaaca gtgtaaatag
    24301 catgagatta catcttaaag attatgcaag agtacatgga attataactg atggaggtag
    24361 catacataat ataattcctg aaaaatctac agctatattt aatgtaagag ccttaagtat
    24421 agaatattta aatgagattt gtgaaatgct taaaaattgt gctaaaggag ctgcaatttc
    24481 tactggaact aatgtagaaa tagttcaatt agatgaaata tacaaagaga ttaagaatga
    24541 ttctgaatta gtgaatatag ttagacaaaa ttttgaagtt ttgggtgaag actatgttga
    24601 aagagattta agtcaaggta taggttcaac tgatacagga aatttaactc atgagatacc
    24661 tgcaatacaa gcatatataa aattaaaaga aaatactgca actcatacag atgaatttgc
    24721 tgttgcagct ggtggtgaag aaggcagagt agcacttata aaagcaatta aggtgttagc
    24781 aatgtgtgga gtagatattt tgtatagtaa ataatatata attttaaaat atattaagaa
    24841 aggaaatcgg attagacatg atattaaaac aagtaataga ggtttatgac attttagata
    24901 aagcaaatgc taatggggaa gaagtaaaaa cttacttaca aggttatggt gaagtagatg
    24961 taactgtaaa agaattgtct tcaagtaaag gttcaacaga tttagtaaag cttacaattc
    25021 ctggaaaaaa tggaaaatta aaaggtggag atgctcctac tcttggtatt ttaggtagac
    25081 taggagggat tggagcaaga ccagaagtta taggttttgt ctcagatggt gatggagcat
    25141 tggtagcaat tgcagtggct gctaaacttt tggatatgca acgaaaaggg gatatattag
    25201 atggtgatgt tgtaatttca actcatattt gcccagatgc tccaactaaa gaacactatc
    25261 ctactccttt tatggattct ccaattgata tgatgactat gaatgaaaat gaagttgata
    25321 gtagttgtga tgcaatactt tctatagata ctactaaagg aaatagagta atcaatacta
    25381 gaggatttgc aatatcgcca actgttaaag aaggctacat attaaaaact agtgataacc
    25441 ttttagatat tatgcagact gtaacaggga aatctccatt tgtattccct ttgtctattc
    25501 aagatataac accatatgga aataatctgt atcacttaaa tagtatttta caaccagcag
    25561 tagctacaga tgcaccaata gtaggagtag caattacaac agagatgcct gttgctggat
    25621 gtgctacagg tgcaactaat tttagtgatt tagattcagc tgggagattt gcaattgaag
    25681 ttgccaagct atttggtaga aataagtgtg atttctatga taaagaagag tgggaaatgc
    25741 ttattaagag atatggcaaa ttaaatagat ttcaaacatt tggtatacag taattagaaa
    25801 aatattaagt ttatataatt tgtgatataa ctatttaaga atatataaac aattaggaat
    25861 aaaagtaaga ataaagcact tagttaaaaa ctttaaaaat aaaatattta tttgtttata
    25921 taaaaaggag gattctggtt attgggattc tcttttttat gaatacaaat aatatattgt
    25981 aattataaaa aaaggagcgt ttcaaagtga tttttttaat caccttgaga caactcctta
    26041 tatttttatt taaattctat tttttattaa gttgctggag ttccaccacc atcaggagga
    26101 gtagtagagc caccaccatt atttccacca ccatcaggag gagtagtaga gccaccacca
    26161 ttgtttccgc cattatcagg aggagtagta gagccaccac cattatttcc gccattatca
    26221 ggaggagtag tagagccacc accattgttt ccgccattat caggaggtgt agaaccgcca
    26281 ccattatcac ctgtgttatt attgttatta ttattttctt ctggttggtt attgtctggt
    26341 ttttttgtat cttcagatga tggttgagaa cttactttag taggagcagt accttcaata
    26401 aatgctgctt tagagccaga agattgcttt cctccatcct ctaaattaat ttttgtaaaa
    26461 tatacatttt taggaacttc aaattcagta accgttaaat tagcatgtat tttttccata
    26521 attttttccc atagttttgc agtactagta cttccatgtg gtacttctct acgttttata
    26581 gtgttaccag aatcatcttt tctaccagca tcatctccaa tataagttgc accaacataa
    26641 taaggagtgt aaccgacaaa ccaagcatca tatgcactat ttgtagtacc tgttttacct
    26701 gctacaggca ttccctttgg aagtgtagca acaccaccag taccttcagt aattacgctt
    26761 tctaacatat ctgtaacaac ataagctact tcagggtcta caactttatg ttcttcagga
    26821 gcatttttaa ctaataattg accatcaaag gtagttatag tagtaaaaat agaaggttct
    26881 acataaatac cgtgatttgc caatgtacca taagcagcag ccatttgaag tggagtaata
    26941 ccattcgcca taccacctag tgtcaatgat gggaattttc tatcacttgc ttcagatgaa
    27001 cctgagtgat tattctttaa agttgttata ccaaagttct ttaagtaatc catcattata
    27061 tctacacatt catcataaga gtctccaagc atttcagcag ttttgacagc tatcgtgttt
    27121 gaagatttag caagtgcttt acgtaaactc atagaaccat gaccagcagt agtagttctt
    27181 ggattccatt tattattttc ttcaaactta tatcctcctc gagaatcact tagagcagtt
    27241 gcttgtgtta tttttagagt atcaatagct ggcgtatata ttgctaaagg ttttatagaa
    27301 gaacctggct gatgagggtt agtagcacga tttaaagttt ttctaccact tatatttctt
    27361 cctccagcta gtgctcttat ttcaccattt ttataatcta agattaccat tgcagcttgc
    27421 ggttggctaa ctccatcagg accgaccata tgtcctggga aattgctatt tctatcaaat
    27481 tcttcttcta aagcatcttg cattttaggg tctatagttg tgtttacaat tagaccacca
    27541 ttgtagaata agttttgagc ttcatcattt gtatgtcctt gttccattaa agcatttata
    27601 acttcacttt cgataaggtc ttctacagat gaagaaactt tatctgatga ttgaggcaat
    27661 tttatttcta ttttagcttt gattgcttca tcatattcac tttgttttat atatttcaat
    27721 tctaacattt tctttaaaac aatttcttgt ctttttttag cttcaggatt acttacagcc
    27781 tttctaacaa ctaatgtacc tgcttttaat tgtctataag tatcattaga tataagctcc
    27841 caagatttta ttttttcaat catatcaaag tccactttag tagggtcatc taaatcatca
    27901 tcagttgtgt taacaaaaaa taataattta ttctctaaat cctccttagt ttcatttccc
    27961 tctaacttag aagttttata ggctgaaaat cttgatggat ttttagttga accagcaaga
    28021 agtgctgact ctgctagagt aaggtcagct gcagacttat caaaataacc acgtgcacca
    28081 gcctcagcac ctgctagacc tctaccaacg aagaagttat ttaaataagt ttctagtatt
    28141 ttatctttac ttactgtttt actcatctca tgtgcatagt aaatatcctt tattttacga
    28201 ggtatagttt gctgttcagt tgttaacagc attttggata cctgcatagg aatagtacta
    28261 ccaccttgct tagtacctgt aagggtcttt aatactgacc taaataaacc tttaatatcg
    28321 acacccttat gctcataaaa acgctcatct tctattgcaa ttacagcatg ttgaagataa
    28381 ggggaaatct tatctaatgg aacgggtgtc tttttattta cacttggagc tgtagataat
    28441 gtttttccat tagcgtattt aatagtagtt gtttggttcg tctgtttatc taaaacagct
    28501 tctgtaactg gagttacatc tcttagtgac gcaaatacta gaccagcagt acctgctgta
    28561 ccaacaacta gtaaaactaa aaatactatc ccaaatactc ttagtttttt aaatttatcg
    28621 ctttttttag attttttctt gttttttgtt gaacgagtct tgttagctga agttctatta
    28681 attggtttat ttgtattacc agatgagcta actttttttc tcctgatttt atttccattt
    28741 ttatcattat cgttattcat aagattttaa cctccttact taatgcaata ttataacaca
    28801 aataggagta gttttgtaat taatagaaaa aatgaatttg ctataatatt ctgtacattg
    28861 tatatgtata aacgtaatat attaaaaagt ctatattttt attttattta tttttgacaa
    28921 atattataaa aattatgcca aagctacata taaataaata ggaggggata attttgaata
    28981 gaatagtaga tagaaaaaca aaaaatgaat ttagaaaaat catagctatt tttttagtaa
    29041 ttcttttctt gtctgtattt ataggcagtt ttatatatat agataaaact ttaagaccga
    29101 caataacagt tttagcagag actaaagctc ttgaactagc aaataggtcg ataaataaag
    29161 ctgttgctga gatggtagaa ggaaaaataa attatgaaga tttaatggat atacaacttg
    29221 ataataatgg taaaataact atgatacaag caaacactat aatgatgaat gaaattgcat
    29281 ctgcaatagc tttagaaata caagatgaat taaaaaagga taagacagca agttcttaca
    29341 tacctatagg aacagcatta ggaagtccta tattagctaa atacggtcct aaacttgaag
    29401 tatctattga acctatagga acagtatcag taaactttaa aacggaattt gagtcttcag
    29461 gtataaatca aactagacat agaatatatt tagaagctca aacacaagtt aaagtagtaa
    29521 taccacttat aacttcaaca aaacaaatta aagctcaaat acctatttgt gaaactataa
    29581 tagtaggtga tgttccagaa agttatgtaa atattccaga aaaaaatcta ggaaatgttt
    29641 tacctaacac tggaaagaat accaacaagt aatgtaaagt tgtatatttt taaataaatt
    29701 caaaataata gtttataaat aaaatttttg gaaataatat taatataatt ttaataaaaa
    29761 tttatatttt atactatgta aaatatctaa tatatttggt ctgtaggtta aataaaagta
    29821 cctttagacc aaatatatta ttaataactt aataacatct taaaacttat ttatttatat
    29881 tatttacaac atccatttaa taaatacacc ataacctaca gctgggtcgt tttcaattgc
    29941 atgtgacact gctccgatta ttttattatc ttgaactatt ggagtaccac tcataccttg
    30001 tacaatacca ccagtttgag ctaaaagctt tttatctgta attttaattt taaaactttt
    30061 actttctgga tgtttttggt tttctatatc tataatttca atattatatt ttttacactt
    30121 attaaagtta tcttgtaaaa ttatttgagc ttttcctaat ttaacttcat ctaaagatgc
    30181 tactttatat ttttttaact tggaaatatc aaagttatta atttgacctc ttattccaaa
    30241 atcagtattt tctgtaaagc taccaataat attttctcgt tttgcattta tacatccaac
    30301 actaccttta taagattttt ctatattaag gtgcttagtt gtagaaattg aacctgtttt
    30361 aataggtatt tttcttgcat taccaacact tattggatga cctacagctc caaactctga
    30421 tgtatctgga ttgatatacg ttaaagtagc aaatccagaa agtaaattgt taaaactaat
    30481 tttttctaat atgtgtttgg tagttttcat tgttataagt tgtccacctc tatttaataa
    30541 aatagatact tgtttttctt ctggtaaagc atttaaggta ttggcaaagt cactatatcc
    30601 atcaacttca gcattattta cctttactat agcatcaccc aatctaaatg gagatgcttc
    30661 tccaggattt ctaactatga tattttttaa ttctgcgtca atttgaagta cgtttccaat
    30721 tggtattaaa tattcaggtt catttttttg taaattttgt gcataaatta aattatttga
    30781 gaaaaaatat aataaaaata aaaatgttaa aacaattgta aattttgact tagctaagtt
    30841 ggttttttta cttaaagtat tatttttaaa atgaaaattt ttaagttgca taaaacacct
    30901 ctttcttatt aaattatttt aataatagaa ttgaagtttt cttgctgctt ctatattatg
    30961 ttgtgcaaga atcatgtttt gatgcttgga aaactagctc aataacacct tagttttcat
    31021 aaaaataaat aaaataaata gtgtatatga taaaataaat acatattcat ataaataaaa
    31081 ataaacaata actgtctagc ataagaaagg agcaaaaaat gaaaaaatat agattgttag
    31141 ttacttgtat aattgccatg ggaatcataa ttataggaac atcaaatcca gttagcaatt
    31201 tttacaagac tacaaatttg attagttcag gaaatatcac aaatgaaaaa gcagataaag
    31261 atgaaaattc aaacaaaaat aataaagata aaaatgaaga caaatctaat tctaaaagta
    31321 ataaagataa gtcatcagag gacaaatcgt cagataataa aaatgaaatt caaaaaaata
    31381 aaaaactttt aatttgtata gacccagggc atcaaggaaa aggagatagc aatttagaac
    31441 cagtagctcc aggttcttcc tctaaaaaag cgagagtatc ttcaggaaca gagggtattg
    31501 ctacaaaaaa gccagaatat gttttaaact tagaagcatc tcttgtttta aagagtatat
    31561 tagaatctaa aggttataat gtaataatga ctagagagac acatgatgta aatataagta
    31621 attcagaaag agcaatactt gctaatgaca aaaaagcaga tatggttgta agaatacatg
    31681 cagatagttt aaataattct tcaaaaactg gagcatctat attgattcct gaaaaagatg
    31741 gaaaatatac agctcctata tatgaagaaa gtaataaatg tgctgaattt atcaaacaga
    31801 atatggaaca atcaggtata caaatcaatg gaatcgttca aagaggtgat ttgactggat
    31861 tcaactggtc aaaagtacca gctgtattag tagaaatggg atttatgagt aactataatg
    31921 aagaccaaat gatgtcaaat ccagactacc aaagaaaaat gatgcagtgt atagctgatg
    31981 gattggatgc gtattttaaa taagatatga atagagaaaa agttaggttg gtattatttg
    32041 cctaattttt atctctattt tttattgtac ataacacaaa gaagaagttt attttaggtt
    32101 aaattgtcgt aaatataact aaaaatattg ggaatacttg atttaagttt aaaaagaata
    32161 tattatagta tagaatgtgt aaaatttttt tgagaggtgt tgaaatgcta gtagtagaaa
    32221 atgtgagtca tggatttggt gcgagaacaa tattagaaaa tgtatccttt agacttagaa
    32281 aaggtgaaca tattgcacta gttggtgcta atggagaagg aaaatcgagt tttttaaata
    32341 taataacaaa gaagcttatg ccagatgctg gaaatataaa atggtcttca agagctacag
    32401 taggttactt agaccaacat acggtattaa gcaagggaaa aactataaga gaagtgctta
    32461 gagaggcttt taagcatatg tttgacttag agcaagaaat gattgctatg tatgacaaaa
    32521 tgggagaagc tagtgatgat gaaatgagta agcttcttga agaaacagca gagatacaaa
    32581 ctatacttga aaatagtggt ttttatatga tagatgcaaa gatacaagaa gttgcaaatg
    32641 gtcttggtct tggagaaatt ggtcttgata aagatgtaac tgacttaagt ggagggcaaa
    32701 gaacaaaagt acttcttact aaacttctac ttgaaaatcc aactattctt atattggatg
    32761 agcctacaaa ctatttagac gaagagcata taacatggct tactaagtac cttcaagagt
    32821 atgagaatag ttttgtctta gtttctcatg atattgaatt tataaataat acatgtaatg
    32881 taatatatca tatggaaaat ggagagttaa atagatataa aggaaattat gatgaatttg
    32941 taagacttaa tgatataaag aaacgtcaag aagaacaagc ttatgataaa caagttgaag
    33001 aaagaaaaag actagaggat tttgtagcta gaaataaagc tagagtagca actagaggaa
    33061 tggctaacag taggcaaaag caacttgata agatggaaat attagaaaga ccaaaagaaa
    33121 aaataaaacc tacttttgct tttaaagatg ctagagcagc tagtaaaata atttttgaaa
    33181 cagaaaattt agttttaggg tatgatgaag cattgacaaa accacttaat ttccatttag
    33241 aaagaggaaa aaagattgcc ttaaaaggaa tgaatggtat tggaaaatct accctattaa
    33301 aaactttact tggaataata aaaccatttg aaggaaatgt taaattaggt gactatttgg
    33361 aagttggata ttttgaacaa gagagttcaa gagaaaatag taatacacct atggatgaag
    33421 tttggagtga atttccaggg cttacaaact ttgaagttag acaggctctt gcaaaatgtg
    33481 gacttacaaa tgaacatata acgagtcaaa tgagagtatt aagtggtgga gaagctgcta
    33541 aggttagact ttgcaaagta atgttaaaaa atataaattt cttagtatta gatgagccta
    33601 caaatcattt agatgttgag gcaaaagatg aattgaagaa ggctatcaaa gagtttaagg
    33661 gaacagttct tttagtatgt catgagccag aattttattc agaaattgtt gatgatgtat
    33721 ggaatataga agattttaca actaaaatcg tgtagtgatt taacttattg ctggggggat
    33781 ttcatgaaaa agtttctaaa tgtacttttt tcaatttgta ttagtattgt tataatagta
    33841 ggtgttatta attttactgt tggatttaaa caactttatt attttgacat tgattactta
    33901 aatatatcag agttatctgg tttatctaaa gatgatataa aattgaacta cgattatcta
    33961 attgattata acttaaataa gaatgtgtct gagttcaaat taccaacatt aaaatcttct
    34021 ccacaaggga agatacattt tgaagaagtc aggaatatat ttcaaaatat aaataaatta
    34081 gctaaattat tattggtagt ttctttagta ggaattatat tgagtgtaaa aaataaaaat
    34141 ataaaaatct taaaaactac atcaataaca cttataatta tgccattatt attaacagtt
    34201 ccaattttat taaattttga aaagtcattt attatttttc ataaattatt atttagaaat
    34261 gattattgga tatttaatcc cgatttagac ccagttataa atatattgcc agaggaattt
    34321 ttttttcact ctggaatgat gattttaata ttaattttgt tagtaagtat agtactgttt
    34381 gtaatgtata aattatgtaa atctttatca ataaaaatat ttcaatgtta aatagaggga
    34441 tatttttatt tccaaaataa gataataaaa aaggattaga ttaaaagtta tacaataaaa
    34501 atagctgtgc aataatattt agactatgaa agtagttgaa aaatatttat gcacagctat
    34561 ttataataga atttactttt tactagtgat tttaactaaa tcttgaaatt tactcataat
    34621 gtaattgtaa ttatcttttt tatgaggtaa tatacaacta ctgcaatctt tgataccttt
    34681 atctgtatac ctaaagtttc caccacagtt ttctcctaaa gcatacaaag gacaatagca
    34741 aaatagacag ttaaactctt ctggtttatt agttttatga caagggaaaa attcgcaatc
    34801 tttgtgatta aaaaacttat aattttcgcc catattttcc tcctaattaa ataaaattta
    34861 aattttttat actatataag attttggtga taatataatt atactaaata attttatgaa
    34921 tagtcgataa tatagtatta ttaatcatag tattaatggt ataattagtc aacagaaaaa
    34981 taatgtaaaa ataatgtaag tgtattttgt aattagaata tgttttataa caaaaaatga
    35041 ggtgattaag tgaaagaaat tcaagaaaaa gcacttcttg taggtctgaa tttgactaca
    35101 atagtgaaaa aaaatgatga tatagacact aatgaatcta tggaagaatt aaaagaacta
    35161 acaaaagctg ctggggcaga agttgtaggt agcttaattc aaaataaaca ctctgtagat
    35221 gcagcttatt atataggaaa aggtaaagta gaagaaataa gagcctatag tgactcttta
    35281 gatgctactt tagtaatttt taatgatgaa ttatctggtg ctcaaatcag aaatatagag
    35341 aatgttgttg gaagaaaagt aatagataga actactttaa ttttagatat atttgctcaa
    35401 agggcactta gcaaagaagg taaattacaa gttgaattag cacaattaaa atatagattg
    35461 ccacgacttt atggaatggg tggtgagatg agtagaacag gtgcagggat tggtactaga
    35521 ggtcctggag aacaaaaact agaaatagat aagagacata tacttaataa agcagcagat
    35581 ataagacgtg aacttaaaga agtaaaaaag aatagagaga cacagagagt taagcgttta
    35641 aagtcaaata taccgattgt agcacttgtt gggtatacaa atgcaggaaa atctaccctg
    35701 ttgaacgaat taataaagac acacaaggat tatgaacaag aaaaagaagt ttttgtaaaa
    35761 gatatgcttt ttgcaacttt agatgtaact ttgagaaagg ctcttttgcc gaataaaaaa
    35821 gaatttttag tagtagatac tgttggattt gtaagtaaac ttccccatga tttagtagaa
    35881 gcgtttaagg caacattgga agaagtacag tatgcaaatt taatcttaca tgtaattgat
    35941 gcaacaaata ctagttatga attgcaaaaa agtacgactg aaggtgtatt aaaagaactt
    36001 ggtgtaaatg ataaaaaaca tatacttgta tataataaag tggacaagct agaattggat
    36061 atatacccta aaagtcaaga agatattgtg tatatttctg caaaacaagg cataaatatg
    36121 gataagttat taaatatgat agaaattgct ttaatggaaa atacttattc agtaagtttg
    36181 atgttacctt atgagagagg tgatatattt agtagaataa aagataaata taatgtggag
    36241 aattttgagt atggggaaag tggtataacg ctagatgcag atttagatga agaagatttt
    36301 aatatctata gagagtacat actagaaaaa taggttgaat atggatataa actgggaaaa
    36361 tatagataat cttgaagatt attttataac ctatcttctt tataaagaat ctaaaacagt
    36421 atctcaaatt agtaaaatta gaaatatttc aagtactgaa gcaagtgagc aacttataca
    36481 agcaaagtta aaaataaagg aaatgcaaaa agatgatttt gaggcatcta aggatatttt
    36541 ggataaattt ctagaactag ataaaattaa gagacttgat tttatggata gcttagatga
    36601 cgaaaaaatg gtatacttta agagaaaggt tttcaaaaga atccttgtag aaaaaaatgc
    36661 tgaagattta attgttttaa tttgggcgac aggtgagcta aaagatgata ggtttttgaa
    36721 attgcttcat cagctcacaa accacagaca ctcagatatt agaagaataa cttattcagc
    36781 aatcagaaag attgaatcac caagtagtag agaagtactg caaaaaggtc tctacgacaa
    36841 aaatgcacag actaggcagt actgtgcaaa agctctttca aaattgggag atgaaaacag
    36901 tttaaagatt ttacagcagt tgaaagacaa gaataagaat tttgaaaaag aatatgtgct
    36961 tagagcttat gacgaagcca ttaagtcatt ggaaaatgca aaaaatttga aaaagtaatt
    37021 ttttattaaa gcaatttgtt tacttaatga tgtatttcta aagtttaaca aggtaataaa
    37081 taaattaatg ggaggggttt caaatgatag taagacgttg tgcaggtgga gtagtgtttt
    37141 atgctaataa agtacttatt gtaaaaaatg ataggggtga atggacactt ccaaaaggaa
    37201 agatactagg agggggactt ccatatgaga gtgctgttca aagagttaaa gtagaaactg
    37261 gcatagatgc taaaatgata gatgtggctg gcgatactat gtatgaattt ttctcaagaa
    37321 gtagacaaca agaagtttgt aatgcaataa tgtggtatgt tatggaagct tgtaacactg
    37381 attgtgtact tgctcctgaa tttcaagaag gtggatttta taaagtaaag gatgctttag
    37441 aaatgttatc acatcataaa gagcaagctt tagtagaagt atcttataaa aagtttaagg
    37501 aattaaagaa attagtttca gagggcgatg atgttccagc aaattaattg tgaatttaga
    37561 ggattctgag gaagggatag ccccttcctt ttttaattga agcagtttta agcgtaaatt
    37621 atatagtctc tttaaactat gatttatcaa tttagagatt tagtagttta tatttaaaat
    37681 cagtaagata tatttaaaat aaaaattaaa aagtatacaa ttttattata taattgtctt
    37741 atgatgttga ttaaagaagt attaaaagtc catttatttt taaatagaat gattttataa
    37801 atatatattt gatgtgtata ttagaaaatg attaaaatag gaggaaatac aatgagcaat
    37861 tacaggacat tacatgaatt tggaacggat gaaataacga ttgaaaagtc tgtttttatt
    37921 ggatatgcta agcctataaa gagtgaagaa gaagcattag agtttataaa tgaaataaag
    37981 aaaaagcata aagatgcaac tcataatgtg tgggcttata ctgtaggtaa aaatatgaat
    38041 atacaaagat atagtgatga tggagaacca caaggaacag ctgggatacc tacattagag
    38101 gttataaaaa aagaagattt gagagatgta gcggttgttg ttactaggta ttttggagga
    38161 acaaaattag gtgcaggtgg tctagttaga gcttatacaa aaggtgctaa actgggactt
    38221 gaagcaggta agataattta taaggtaatg tatcaagaag ttaaggttaa gatagattat
    38281 actcaacttg gaaaagtgca aaatgagtta atgaatttgg ggtattttat aaaagatact
    38341 gtatatgagg ataatgttga aatagtagta tattcaagat tagaagatgt agagaaatta
    38401 tcagaaaaaa tgattgatat aacaagtgga acaggaaaaa tagttttagg agaggaattt
    38461 tatttgtctg aacaaaatgg agaaatttta atataatcta aaaggttatt agaatagtta
    38521 atacctcaaa tagccaatta gtatttttaa gtgaaaagcc attaaattga tttaatctaa
    38581 atcaatttaa tggcttttta tcggattttg cacagaatcc tttttttact tcaaataaac
    38641 cgactaaaag taaaaatatc ccaaatattt tttttaatat atctgaagac agataattag
    38701 ctaatagtga accaaataca gctcctaaaa ccccaaacac agctataggt ataaccaatt
    38761 taaaaattac atttttattt tttatatgta tagcaagtgc tataagtgtc atagggatag
    38821 atgaaagtaa atttacgctt tgtgctattt tagtgtctat gccagcaagt aaaacgagtg
    38881 caggtataag gattgttcct ccacccattc ccattcctcc aataattcct gcaaaaaatc
    38941 caattagagc aaataacatt aaaacaccat ccttaatgct gcaattatca taattattcc
    39001 aaaagaaatt ctaagaaact ttccagtaac cttatttagt aatttagctc ctataatacc
    39061 accaagaata cttccaactg caactttaat tgttaagtta acatcataag tacctttaga
    39121 tacatataaa acagaacttg ctgtcgttaa gaatattatt atagaaagag cagttgcatg
    39181 agatttatgt tcttctacct taacgatgtc atttaatatt ggaactaaaa gagtacctcc
    39241 tccagaacca aaaactccat ttataaatcc tgtaaaaact ccaattatcg tattttttaa
    39301 attaaaatca aagtattttt tctttaaatc atcagttttt ttcataaaaa tcaccttctg
    39361 aaatagtttt accaaaatag gcaaattata tttatataaa ataagattaa atataatctt
    39421 tcatgtttgt ttaattttgc ataaaaaaaa acatattaaa acaacatatt aatatatggt
    39481 ataatatatt aaaacaaagt agcaaatttg agaggagata acaatgtcaa atataaaaat
    39541 acaaatagac aaaactgtag aatggcttat aaataaggtt aatgaggcaa atgcaaaagg
    39601 attaattgtt ggtgtatctg gaggaattga ttcggcagta gttgctaatt taattaaaaa
    39661 ggcatttcca gaaaactcta tgggagttat aatgagcata aaaagtaatc ctcaagatag
    39721 agaagatgca cttaaggtta tagagggatg tgatatagaa taccttgatt tagaccttat
    39781 agaaccacaa agtgctatat tagacatggt agtaggaaat ttaaaagata aacatttata
    39841 tagagaagaa tatttaaaga tgacagatgc aaacttaaga gcaagagtaa gaatgagtac
    39901 aatttataca attgctaaca atctaggata tctggttgtt ggtacagaca atgctgcaga
    39961 aatacacaca ggatatttta ctaaatttgg tgatggtgga gttgatattt tacctattgc
    40021 aaatttaact aaaggagagg tgtatgaatg ggcaaaagag cttggtgttc atgaagattt
    40081 aataaacaaa gctccttcag caggtctttg ggaaggtcaa acagatgaag atgaaatggg
    40141 aactacttac aatatgatag atgctgtatt agaaggtaga ttggatgaag ttcctaaaag
    40201 agaccaagag ataattgaaa gacttcatag attaagtgaa cacaagagaa aaacgcctgc
    40261 acaacctcct aagttctaat ttactattta taagatttgt gataaaattt acaagtgatg
    40321 aaaattagaa taattaattg tacgtattaa ggagtggaaa tatgggacgt ataggtaata
    40381 taattaatag aaaaggtaaa caagatgctc aaagagcaaa gatatttact aaacatgcta
    40441 gagcaatagc agttgcagct aaggaaggtg gagcagaccc agagtacaat gcagcgttaa
    40501 aaacagctat agaaaaagct aaggcagata atatgccaaa tgataatata gatagagcta
    40561 tcgctaaagg tgctggagca ggtgctggag aagactatga aactatagtt tatgaaggat
    40621 atggacctgg tggagttgct gttatagtag aaactttaac tgacaacaaa aacagaactg
    40681 ctggtaacgt aagatactat tttgacaaaa acggaggaaa cttaggaact agtggatgtg
    40741 tgtctttcat gtttgataaa aaaggacaaa tattagttgg tttaggagat ggagtttcag
    40801 aagaagaatt gatggatgtt gcattagaag caggagctga agatttcata acagaagaag
    40861 atggttatga aataataact acaccagaag atttttctag tgttcgtgat gagttaaaag
    40921 ctaaaggata tgagtttata tctgctgatg tgaagatgat acctcaaact acaactgtat
    40981 tgactgaaga atctcattta aaaatgatga ataaattagt tgatatgctt gaagaagatg
    41041 atgatgttca agatatatat cataactggg aagtagaata aatagtataa gcatattgaa
    41101 atttataagt attgaatcaa ttttaacatg tgataaattt tatagaataa gaaacactaa
    41161 aatagacata cgcacctcta acctatattt gcataataat atgtaagcat aggtaagggg
    41221 tgtttttata tttataggtt aagaaattta aagtaaattt tgagctggac tcgattgtct
    41281 ataaataatt gatgggtata taacttgttg ctgaatacca tttatacgcc cagttactgt
    41341 atcaataatc atcctaagac tacgttctgc tgtctcttcc atttttaaat ctataactgt
    41401 caaaggagga atacttcttg ccattagtcg tgaaggtcca ttacttgctg caaatatttg
    41461 aatatcctct ccaattacaa ttcccgattc atgaaatgca ttcatcatac caagagctac
    41521 cacatcatga ttacaaaaaa ttactttttt tagtgaattt tttgcaagca ttttttgagc
    41581 aatatcatat ccactatcta ttttattaga acaataatat atagaatctg atagatgtat
    41641 tccatgttct ttacatgttt ttaaaaaaga tttacccctt aaagtcatac atgtaaagga
    41701 agttgggttg aatacaacag caatatcatt tttaccattt tgaatagcat gtaaagctgc
    41761 tatacgacca gtttcctcat tatcaataga tacacttgaa aaaccaggta tttcacgatt
    41821 tacaacaact acaggaattt taggtttatt ttttactaga tattctatat catcagcatt
    41881 gatacctgtt aaaacaattc catcataata attatttgca aaaaagtatt catcatcaca
    41941 tagatgacca actttgtaag gtcgtatgat gatatttatg tttaaatgtt ctaaagaaat
    42001 tatgtggttt attgcttgaa gtatattggt tgatgccaac tcaaatccct catcctgcca
    42061 ataaaaagct attttaatat tattatgttt aataggagta gagcgtttat atccaagctt
    42121 atcagctgaa tttaatataa gctcttgtgt atgtagcgga atcctgcgtt catttccttt
    42181 accacttaag acgtacgaga cagttgctga agaaagacca cagtcttttg caatatcttt
    42241 gattgttgcc atgataaaat cctccttaaa tagttgctta aatttgctta taccaataat
    42301 cttaacatat attcaactaa agaacaatat tctacaaggt gcatatttta caggattaga
    42361 ataaataaat taaaaaaaac ttcttactta tgttgacata atttagttta taggattata
    42421 atatgcttaa ataagcttat gattaacttg aattaagcaa agttaagcag gaacatgcaa
    42481 tgctagcttg caatgtgact gcaaaggggg aaaaggtatg agtaaggtaa atatatttga
    42541 agttggtcct agagatggat ttcaaaacct gaaagagttt ttagaaactg ataaaaagct
    42601 taagattatt gatggtctta ttgcttctgg gattaaacaa cttgaaatta gttcatttgt
    42661 aagcccaaaa gcaatacctc agatgaaaga tgcaaaagaa gtagcaactt attgtgtaga
    42721 aaaatatcct gatgtgaaac tttatgcatt agttccaaat ttatatggtg ctaaagttgc
    42781 atgggagtgt ggtataagag atatatccta tgttatatct ttgagtgaaa ctcataataa
    42841 aaaaaatata aatcgtaccc atgaagagtc tattgatgag cttaaaaaaa taatgaatac
    42901 ttatccagac atgaatatat gtattggaat ggctactgta tttggatgtc cttttgaagg
    42961 tttaccaaaa attgaagatg ccataagatt tgctaaacgc ctttgggaaa acggtatcag
    43021 ggagataaat ctagcagata caatagggat tgctgaccct aagcaagtaa gagaaactat
    43081 tagtgcatta gttgaagctt taccagaatg cagatggcaa gtacatattc atgatactag
    43141 aaatatggga atggttaata ctcttgtggc tattgaatct ggaattacta ctgttcagtc
    43201 tacacttgga ggattaggag gatgcccatt tgctcctgga gcatctggca atactgcaac
    43261 tgaagattta gtatatatgc ttgaacgtat ggggtatgat acaggagttg attttgttaa
    43321 acttcttgaa ttatcaaaat atcagaagag ttttgtaaat ggagtatata gtggtcatca
    43381 tgtaaatata aagtctgata gttgtaattg ttagttctat tgattaatcg attaaaaaaa
    43441 tatgctgatt ggtaaaatag atttattgat aaaaaatata aattataaaa tacattatct
    43501 aataaaacaa ataaaaaaat tgagggggat tagtatggat attatagctt tacttttatt
    43561 tttaggagtt attattattg catttgtacg taagaataat gcaggtattt tggctctagc
    43621 tattggtgtc atagctgtta gaatttttgg aatggatgat aaagaacttt atggagctat
    43681 aagcaactca atgtttacta cacttgttgg tataacacta ctctttgcaa tagtgaactc
    43741 aacaggagca ttaaagcttg ttgcagaaaa aattgtagct gcttctggta aacgtgtttg
    43801 gcttatacct atagcagttt ttatagcagg atttgttgtt gctggtgttg gacctggggc
    43861 aattccagca cttgcaatta tcccagcaat ggctgttcct gtagccataa cagttggtta
    43921 taatcctgta atgcttgcac ttataggtga atgtggatta atggcaggtc gtatgactcc
    43981 tattactcca gaaggtcaaa ttataacaac cgttgcagaa ggggttggaa ttgaaaatgt
    44041 tatgcctact atcttagcct gtcaaactct ttctacaatt gtattttcaa tagtattgtt
    44101 tataattttc aaaggatata aacttaaaaa acctataaat gttatagata gtaagcaaat
    44161 agaaaaattc aatataaagc agataatttc tctaataagc atagtagtta tgattgcttt
    44221 tatcataatt tttgatatga atgttgggct tgcagctttt gcagtttcag ctgtacttat
    44281 ttttttcaat attgctgatg aaggagaatg tatcaaatca attccatgga gcacgatagt
    44341 aatggttctt ggagtaggtg caatgatgac aatcgtagat gcagcaggag gtattgactt
    44401 gatgagtaat gcattatcat cactgatgaa tagtaaaaca gctactccaa ttatgagtat
    44461 ttcagcagga cttatgtcta tggttagttc tgctttagca gtagtttatc caactatgat
    44521 gcctatgtgt gttgacatag ctaaggcagt tggaggagta gacccactag cacttattgc
    44581 tgcagttggt gttggaggtt ctcttgctgg tgtaagccca ctttctactg gtggggcatt
    44641 gattttagca gctatgggtt caagtaagaa agactttaat aaggaagaac aaaataaggt
    44701 ttttgtacag cttttcatta tgagtgcagt tggcttattg gttattgcag tagtatcaat
    44761 attatcattt aatgcaataa cacatttaat gaactaatta gaataactga aaataaatca
    44821 ttaaatttaa cttttaatat taatcaatat aatataattt ggaggggaag ttatgaaaaa
    44881 aggagcatta agcaatataa aggtacttga ccttacaaga gttcttgcag gtccatattg
    44941 tacaatgatg ttggcggatt ttggagctga tgttataaaa atagagatgc caagaaaagg
    45001 tgatgataca agaggttttg gaccttttgt aaatggagag agtttatact atgcaaatat
    45061 aaatagaaat aaaaaaggta tcactctaaa ccttaaatca gaagaaggaa aaaatatatt
    45121 tttagaaatg gtaaaacatg ctgatgttgt agtagaaaat tacagaccag gtgtaatgga
    45181 aaagttaggt ctgggttatg atgagttggc taaagttaac aatcaaataa tttatggagc
    45241 tgtatcagga tttggatgtt atggaccata ttcagaacgt ccaggttatg atattattgc
    45301 acaggcaatg ggtggtctta tgagtataac agggcaagaa ggaggagaac caacgagagt
    45361 tggtaatgct atgggtgatg tactaggtgg aatgaattta actattggta tactcacagc
    45421 actcaacgct agaaaaataa taggaaaagg acaaagagta gatgtttccc tcgttgattc
    45481 tgttgtatcg tctctcgaaa ctggtataca gagatacttt gattcaggac aacctgaact
    45541 tatgggtaat agatatgctt cagcctatcc ttatgactct tttaaggcaa gtgatggaag
    45601 ttttgtaata ggctgtggta atgacaaact ttttgaaaag ttatgtaaaa aagttctttt
    45661 tagagaagac cttttaatag atgagagatt taaaacaaat cttaatcgtt gtgaacatta
    45721 caaggctctt aaaccagaaa tagaaaaatg gtctgttaag catacaataa aagaagcagt
    45781 tgacttaata tcagaggctg gagttcctgc tgctcctatc tataatcttg attgtgttac
    45841 aacagatgaa catatagcaa aagcaagaga aatgattgta gatattagtc atcctattat
    45901 aggaaatatg aaagtcaatg gaaatccagt taagttgatg acaacaaaag ctaaaattga
    45961 tatgccagca ccatctctag gggaaagtaa cgaggagatt tataatggtt ggcttggttt
    46021 tgatagtgat aaaataaaag atttaaaaga aaatggagtt atttgattaa aaaatagcta
    46081 aacacacttg tgactttagt catgggaggt tcagttagaa taatatcaaa aaatattaaa
    46141 tcaatactgt aaataaaata tagaattgtc agaagcattt aagatgggaa aagctactta
    46201 gacattgagt ctttgtggct tttcctattt tcattaagaa ttttatatta tcagaataga
    46261 tatgtactaa actaaagtat taacaattta aattaataga aaataaacta cctaattatg
    46321 ttgaataaag actactttag tgaaaataaa cttatagaga tgctatttaa attattttaa
    46381 tggaaatatc aaaaatgaga ctgcattctc aaaaatgaga ctgcgttttt taagtatttt
    46441 ctcgaaaatg agacaaaaat attgttttat gggttttatt gaagaaaaat tcttctgtga
    46501 attacaattt aatcaatttt aaagttgaaa attattaaaa atttagtgaa atttatgtat
    46561 atttcgttta ttttattaaa aataacaatt ttttactaaa aataaagaac ttcaatggtg
    46621 attggcatag tacttgctat tttatattaa caagaaatgt agaaaaaata acaatgtata
    46681 atggggggaa tatgatggaa tctgtaatgg aaaatttgaa caatcttaag gtggaggtta
    46741 aggacaaagt ctgtgttatt actataaata gacctaaagc tttaaatgct cttaatagtg
    46801 acactcttag ggaactatca caagttattg atgtagtatc agaaaatgag gcaatattag
    46861 gagttattat tactggagaa ggtaaagttt ttgtagccgg tgctgatata cgccagatgc
    46921 aaaactataa aagtgaggaa ggcagaaaat atgcaggtta tgctcaggga atttttgata
    46981 aaattgaagc tcttgagaaa acagtaatag cagcagttaa tggatatgca ttaggtggtg
    47041 gatgtgaact tgctatgagt tgtgatatta gaattgcatc agaaaaggct atatttggac
    47101 aacctgaagt taatttagga gttattccat gttttggtgg aactcaaaga ttatcaagac
    47161 ttgttggaac tggaatagct aaagagttaa tttttacggg gagacaagta aatgcagaag
    47221 aagctaaatc tattggactt attaataaag tggttccaag tgatttactt ttagaagagt
    47281 ctatgaagat gatgaaccaa atagttgaaa aagcgccaat agcaataaga tatgcaaaag
    47341 ttgttattaa caaagggata gatatggatt taaagaatgc tcttgaacta gagaaagatg
    47401 ttgcaggttt aacttttgct acaagagata agcaagaagg aatgaatgca tttatagaaa
    47461 agagaaagcc agtttttgaa aataaatagc tattataact aaaattggaa caatgtagaa
    47521 tatattcaaa atctaaattt cgtgaaaaaa tacatatagt ttaattttaa aaggagttaa
    47581 tttatgaata cgggagtaat tataagttta gtagggatta tcatagctct atctcttcta
    47641 gttgtaatgg ttatgaaggg aataaatatt tttattatag caattgtatg ttcatcatta
    47701 gttgctataa caggaagcct aaatgtgtat gatgcactta aagtggatta tatgacaggg
    47761 tttgttggat ttcttcaggc aaatttcttt attttcctaa cagggacatt aatgggaaaa
    47821 ataatggaga ttactggtgg agcaaaatct attgctaaga tgattgtaag atggattggg
    47881 aaggataaag cactactttc tattccaata gcttgtggaa tattagccta tggtggagta
    47941 agtgttttcg ttgtaagttt tgcagtgttt ccgatagctt tagaaatttt taaagaagct
    48001 gatttgccac gaagatttat accagcagct ttgacttttg gatgctcaac ttttgctatg
    48061 gttgcaccag gggcaccaca aattcagaat attgttccag catcaacatt agggacagat
    48121 attatggctg gagcagtaaa tggttttata tcatgtgcag taatgtttat agttggaagt
    48181 acaattttgt acaaaatggt ttcaaaagaa aaagccaatg gtggtcattt tatagcacat
    48241 gaatcagata tatttgatga agcagaagca actaattcta agagagaaaa tggtcccaat
    48301 ggtgtaattg ccttaattcc attgatagtg tcgattttaa taattaatgt aaaagttaat
    48361 ggaaaagcga tagtaccaat tgaggtggga gtatttattg gagctttatt agtatattta
    48421 ttgttaaata aatatcaaga taatactaaa atagttggac atgttggtga tgcatgtaaa
    48481 actacagtag tagcaatatg taatacttgt gctgtagttg catttggttc agtagtaaaa
    48541 tcagcagttg gttttgactt catagttaat gctatgacta atattcctgg tcctcctatt
    48601 gcaggggctg cagttggaac tacagttatt gcaggtattt gtgggtcagc ttcaggtgga
    48661 ctaggaattg cagctccact tcttggacca gtatttttag ccaagggtgt ttccgctgca
    48721 gcattaacta ggactatggc aatttcatca gcagcattag attcacttcc acacaatgga
    48781 tatattgtta ctgtaacaaa tggtctttgt aaagaaacac ataaggatgc ctatatgcca
    48841 atattctggg taactgtttt aaccccattt attggaacca tagtagcagt cctactgttt
    48901 acaatgttcc cgggattgcc ataggagaaa agataaaagg tataaattat attttgatat
    48961 tataaaatca attttcaaaa tatatacaat aaatttcatg ttttaatact gtaaatatgt
    49021 atttaaaata acaaattgac catataagga ggaattgaaa gtgttaaata aaccattaga
    49081 gggagttaag gttattgacc taacgtattt tgtagcaggt ccagggactt ctaaaatatt
    49141 agctgactgg ggtgctgacg ttattaaggt agagccaagc tttggagacc caggaagaaa
    49201 gacaggggca acaatgacaa tgccaataga tgattataat aatccttttt acagcacata
    49261 taattcaaat aaaagaggtt tgtctataaa tcttaagagt gaaactggaa ttgaaattat
    49321 ggataagctt ttaagcgaag caaacgtatt tgtttcaagt tatagaacag gagcattaaa
    49381 aagactaggt cttgattatg aatcattaag taaaaagcat ccacacctta tctggggtca
    49441 gataaatggt tttggcgatt ttggacctgc taaggataat gcaggatttg atacagttgc
    49501 cttttgggca agaagtgggg ctatgcttga tatagcagaa aaagatacaa gtccaataaa
    49561 tccacctata gcctttggag atgcaacaac ttcttgtagt ttatctggag gaatatgtgc
    49621 agctttgtac cagcaagcta agactggaaa aggacaaaaa gtaatggcat ctttatttgg
    49681 gcaagcaata tggaatgcat catctttatt agcatctaca cagtttagtg atgagtatcc
    49741 aaaaactaga aaaaatgcta tttcaccagt aataaattct tatcgttgta aggatggaaa
    49801 gtggatattc ttaagtattt tagagcatga aagatatttt gaggcacttt gtagattatt
    49861 tggaagagaa gacttaataa aagaagagaa atttgcatct tcagtagaaa gtaagaaaaa
    49921 tgctacagaa cttattaata ttttagatgg tgaatttgca aagtttactc aaaatgagat
    49981 ggttgaaaaa cttactgctg ctgatatagc tcatgaaaaa attcaacatg ttaatgatgt
    50041 agtgactgat gaacaggcta ttgctaataa ctatatatat gaacatacaa gtaaagatgg
    50101 aaataagaca atgcttgcat ctactccagt taaatttgga aatattgaag ttaacatgac
    50161 ttgtgatgct ccacttatag gagaacattc agatgagata ttgagagaac ttggatacag
    50221 tgattctgat atagagactc taattgaatc tggaattgta acaatgaata aagaagcatg
    50281 tgaaatatgt taataaacat gtgaagtatg ttaataaata ggagggaatt ttatggattt
    50341 tagattaaca gaagcacaat taatgcttca aagagtagca aaagaatttg cagagaatga
    50401 aatagcacca atagctgctg aaacagataa aacaggtatt tttccaaggg agctttttag
    50461 caaaatggct aaaataggat ttaatggaat tggaacacct gtagagtata gtggttctgg
    50521 tggggcagat atagaaaagg taattgttgt tactgagatt gccaaaaaat gtgcagcatc
    50581 tgcagcaata ttatcaatac acacaatata tgctcaagct attttgaaat ttggtactga
    50641 ggaacaaaag aaaaaatacc ttccaatgat ggctgaaggt gggtgtgtag gtgcttttgc
    50701 cttaaccgaa cctaatgctg gttcagatgc tgcgagagct gcaacaactg caattattga
    50761 tgaagaaact gatgaatatg ttttaaatgg aactaagtgt tttatttctg gtggaggtca
    50821 agcagaatca ttaataatat ttgcattgac agacccatca aagggaataa agggaatgtc
    50881 tgctatcatt gtcgacaaag gtactccagg tttttcaata ggaaaaattg aagaaaaaat
    50941 gggtattcat ggttcagaaa ctgcagagtt aatatttgat aactgtagag ttccaaagtc
    51001 taacttatta ggaaaagaag gtaaaggatt taatatagct atgacttgtt tagatggagc
    51061 aagaattgga gttggagctc aagctgtagg aattgctgaa ggggctttag aagaaagtat
    51121 caaatattct aaagaaagag tccagtttgg caagccaatt tcagctttgc aaggtattca
    51181 atggtatata gcagatatgg cgacaatggt ggaatcagca aaattattag tatattatgc
    51241 tgcagatttg aaagctagag gagaaaaaca cactaaagaa gctgctatgg caaaatacaa
    51301 tgcgtcacgt actgcaagag aggttacaaa tttagccctt caaatccatg gtggatatgg
    51361 atatatgaag gattatccat tagagagaat gtacagagat gccaaaataa cagaaattta
    51421 cgaaggtact tcagaaatac acaaagttgt tatttcaaga gcagtactag gataggagga
    51481 aaagaatgaa tatagttgtt tgcttaaagc aagttccaga tactaatgaa gttaaaataa
    51541 ataaagagac aggtactcta ataagagatg gtgtaccaag tataatcaat ccagatgata
    51601 gaaatgcttt agaagaagct ctaaaaatga aagatgaact aggagcagtg attaaggtta
    51661 taagcatggg acctcctcaa gctaaaagtg ctttaaaaga agctttggca atgggagctg
    51721 atgaagctta tttaatctca gatagagctt ttggtggttc agatacttgg gctacatcta
    51781 caattattgc tgctgctata gagaaggttg gaaagtatga tgtaatattc tgcggaagac
    51841 aagctataga tggagataca gctcaggttg gaccagaggt tgcagaattt ttagggatac
    51901 cacaagttac ttatgcgaaa gaagtaaagg ttcaagatga caaattacta gtaactcgtt
    51961 atacagagac aggggattat ttaatagaag caaagatgcc agttttatta actgctataa
    52021 aagagttaaa caatccaaga tatccaagtg tcaagggaat cttagaagct tataataatg
    52081 gagatgcaaa gattactgta ttaacacttg cagatttaga tgttgataca actcaaattg
    52141 gattaaaggg ttcacctaca aacgtttata agtcttttgt accagttaaa gacaagcata
    52201 atgaaataat agaaggtata aataaaaaag aaaaagctga gaaactaata gaaatactat
    52261 tcgatttgaa actagtatag gagtgttaag atgatgagag caaaagttaa tcaaggtata
    52321 aatttaaatg attataatgg tgtttgggta ataggagagc aaagggaagg taaaattaat
    52381 cctgttacga tagaacttat tggtgaagga agaaagcttg ctgaccaatt aggaaaagag
    52441 cttgcagttg tgatagctgg atatgaagta gagaaagaag tgaaggaatt acttcactat
    52501 agtgttgata aagtatatta catcaatgac cctcttctta aagatttcac tactgatgga
    52561 tatgcaattt caattgcaaa tttaatcgaa agaaaaaagc cagaggttgt attggttggt
    52621 gctacttcta taggaagaga tatagcgcct agaattgcag gtaaggttgg aacaggtcta
    52681 actgctgact gtacaaaact tgaaatagat tcaacagata ataagttatt acaaacaaga
    52741 ccagcatttg gtggaaattt aatggccact attgtttgtc caaaaaatag accacagatg
    52801 tcaacagttc gtccaggagt tatggcaaaa gctgtaagaa acgaatcaga gactggaatt
    52861 ttagaagttg ttactccaga acttactgaa aaaatgatta gaactagatt agtagagata
    52921 cttccacaag aaaagaaatc tgtaaatcta actgatgcta gaataatagt atcaggggga
    52981 agaggattaa aaagagcaga aggatttgag cttataaaag aattggctga taaattgggg
    53041 gcagagattg gtgcttcaag agcagcagta gattcagggt ggatagaaca ttctcatcaa
    53101 gtgggacaaa caggaactac tgttagacca gaactataca ttgcatgtgg aatatctgga
    53161 gcaatacaac atttagcagg tatgagtgat tctaaatata tagttgctat aaataaagat
    53221 gcaaaagccc ctatttttag tatatgtgac tatggaatag taggagattt atatgaaatt
    53281 atacctgaaa tgatagaatc attaaatagg taacttttag aaaacattga atatgtagaa
    53341 tgataagtct tataatattg tattgtaaga cttattattt ttgctgaagg ttatagtggg
    53401 gagatgaaac aatgaaagag cagtggtata aggatatatt tgcaagagta ttatctatga
    53461 ctgatgatgg ttttattgtt gtaaatacaa gtggagtaat aatagatatt aatgataaat
    53521 attgtgactt tttaggaaaa gaaagaaaag atataatagg acaaaatata caaagtatta
    53581 taccaaatac aaaaatgcta gatgttatga aaaataagta ttgtgaagaa ggagctatac
    53641 atcattattc aggtggaaat acaaaagaaa aaagtgtaat agtaagtagg tcttatgtgg
    53701 aaaatgacaa tggagaagta gttgcaggag tagctcaagt taaatttaga ttgcaatcat
    53761 ttgatgtagc taaaaagtta atgtcagaat atatggagct ccaatattat aaagagcagt
    53821 ttaaggataa ctgtggtttt gacaagctca taggtgaaaa tagagacttc atagaactta
    53881 agaaaacagg tgtgaaagct tctaaaacaa actttccagt acttttaacg ggtgaaacag
    53941 gcacaggtaa agaagttttt gctagagcaa ttcacaataa tagcagtagg tcagataaac
    54001 ctatggttag tataaattgc gctgcaatac cagaagaatt attagagtca gagctatttg
    54061 gctatgacga aggagctttt acaggagcaa aaaagggtgg taaaaaaggt aaatttttag
    54121 ttgcgaataa tggtacaatc tttttagacg agataggtga catgcctttg accatgcaag
    54181 caaaactttt aagagttctt caggaaagtg agatagaacc agtgggaggt cttaaaacta
    54241 taaagataga tgtaagagta atctctgcca caagaaagaa tttaagtaaa atggttgaag
    54301 agggattatt tagagaagac ctatactata gattaaatgt aataaacatt catatgatgg
    54361 aattgaagga caggcaagat gatattttat tgctagcaaa ttatatatta aacaaattaa
    54421 atgttgaata caaggaattg aaggttttaa gtgataaggt taaaaattgt tttattaact
    54481 atacttggcc gggaaatata agagaactac aaaatgtaat aaaaagtgcc tatgctgtaa
    54541 gtgatgatat ggtcattatg atgtgtgacc ttccatctaa gatggataat atcagcagag
    54601 tagctcaatg taatgtggat agcaattgtt caattcatga aatggttgag aattatgaaa
    54661 agagcttaat tattgatgtg ttaaggaagt ataattggaa atgttctaag gcagcagagg
    54721 ttatgggaat acacaagagt cttttatata agaaaataaa aaagtatgaa attgaattaa
    54781 ataattaaga taacttgaat taaggcaatg ctaaaaaagc gcccttattc aaagcgaact
    54841 gtataaactg ttttatgcag ttaaaacgtg taaaagtaga tgtgaagtac taatgtcaat
    54901 cattagtact tttttattat atttataatt actatctata tttcagtatc ttttgatatc
    54961 taagattatt atgtttaatt tagtatataa caaaaccctc ttagttatgg acagtttttt
    55021 atattttaac tgtctactaa gaggggatag tttagtttaa atttagagta aattttatct
    55081 agcatttata aaaaatattt tgaatagctt ttttgttatt tagtcataat tttctacttc
    55141 tttatcaagt tctttgccac aagaatcaca cttatatata gcattgtatc cgtaatcata
    55201 ttcatcaact attttctttt gtctaaatac accaccacac tcacatagga aaccaatttt
    55261 tataagtgta acgataaatt gtgattttat aaattttata tcattgcatt tatcgttagt
    55321 gtaaaatgta taaatataag agttatcgca ctcatctctt aattcaaatc tttcaatata
    55381 tgtgttattt tttaattcct caaattcttc tttagttaaa tatgattttt gttgcaattc
    55441 atgtaaacgc ttaatttggt ccattattta tatcctccca ataattttag tattttaagc
    55501 ttagataagg aatctaagtt ttattaaatt tatcccaagg aattatttaa atcatattta
    55561 gttttattaa ttacaacata tataaattac acgaaaataa aactaattgt aaaaataata
    55621 tcctgtgaaa acctaaacta ctaacccctt actaacccga aaactaaaaa aagaaaatat
    55681 atccaaatat aagtgatata taaataaaga tgtttcatat aaatttaact ttaaatattc
    55741 tgacaataat atatataaat atattatagt taaaatatac cacatatttt gggtattgta
    55801 tactggaatt agagtaatta atttattttt taacatacaa atgattttat gaaaaaaaac
    55861 aagactcaaa aatttttata ttctttgata gaataaaatt gagagtaaat ttattgaatt
    55921 ttataaaatt tataaggggt gagtctaaga tatgcatgaa gataaaaatg atatatttga
    55981 atcaatagat actgaggatt ttgataataa aacagaagaa gaatgtttag agctagtaag
    56041 aaaaaatgga ttgaatttaa tatacataaa aaatcaaaca gaagagatat gtctagaggc
    56101 tgttagacaa aattgtaatg caataaaatg tgttcagaat cagacagaaa aaatatgtat
    56161 agaagcagtt aaacaagatt ggagaatgtt agaatttgtg gaagagccaa cagaagaaat
    56221 ttgcatgttg gctattaacc aagatggaac agcattaaaa tatatagaaa atcagacaga
    56281 ggagttatgc ttaagagccg ttgaaaaaaa tggtgcagct ttggagtatg taaaagaaca
    56341 aacagaagaa atatgtatag aagcagttag aaacagcgaa tttggattgg caagagtgaa
    56401 aaatcaaaca gaaaaaatat gtatggaagc agttaagcat tgtagctata atctaaaatg
    56461 ggtagaaaat caaacagaag aaatatgtat ggaagcagtg agacaaaatg ggcttgattt
    56521 gaaatttgta aaaaatcaaa cagaaacaat ctgtttaaga gcagtaagac aaaatggaat
    56581 ggcattagaa tttgtaaagg aacagactgt tggtatatgc ttaaaggcag taagacaata
    56641 tggaatggca ttaaaatttg taaaggaaca aacagaagaa atttgtacag aagctataaa
    56701 acaagataaa agagcactta gttttgtaaa aggagataag gaaaaatata aggctctata
    56761 tgataataat gaaccatttg caaagagata tgttagaaat gtaatagaaa aagaaaacag
    56821 agcattgatt aaaaaaggag aagaaaatgc aaaaatcaaa attgcaggaa aactttatga
    56881 tagaggcatg tcttttgaag atatatcaga tatagttgag attgaaataa gcaagttaaa
    56941 agtgagttta ggtgtttttt aaagtaataa atgtaggtgt tttttatggg aaatagttaa
    57001 taaaaaaata atcgactagc tctttgtagg acagcaaaat tagtgatata atttgatgta
    57061 taataaatta tttttattag ataaggggga attaaagttg aagattacag tattatacca
    57121 aacaagaagt ggaaacacag aaagggtcgc taagctgata gaagagggcg ctaaaaaggt
    57181 ggatggagta gatgtaaagc ttatgagact tgataatatt gatttagatt atttaaatga
    57241 aagtaaagca gtaatatttg gaacacctac ttatttagca aatacaactt gggaagtaaa
    57301 aaaatggttt gatgaagatt caaaaaaagt taatttagct ggaaaattag gagctgtttt
    57361 tgctacatgt gattatatat gtggaggtcc agatgttgcg atacttacta tagtaggaca
    57421 tttgatggtt aaagggatgc ttgtgtattc tggaggtggt tctttaggaa agccatttat
    57481 acatttagga catgtacatt caattgaagg tccagagtta caagatgaga aggctataat
    57541 atttggtgaa agaattgcta ctaaggcaaa agaactattt gcttaatttc tatatgcata
    57601 gtaatgatta gagtaaattt agggttttaa tggagatttg aagtaaaagt tataaaataa
    57661 aaaattttcc ataaaattac tcaagatgaa ttataatata attataaata aattatatta
    57721 ttaaaatatt taaaatcaaa cgtcaatagc gagggaagtt tatggtatgg catatagaat
    57781 atgagatttt ttctgcaatt atagttattt ttttaatggt atactttttt agaagtaaat
    57841 ttatacctac tttacaaaac aaaatatatt gtgcactttt aatattctca tttttattta
    57901 taatttcaaa tattttaggt tcattttgtc taaataatat agacaaaatt ccaattttta
    57961 ttactttttt attgaatcag atatacttat tgttattgcc aataccagca gcacttgtat
    58021 cattttatgt tatggctatt atttatcaag atataaggta tatgaaaaaa agattattat
    58081 ttttatctat accacttata atttgtataa tcttatctat tacaaatcct tttacgcata
    58141 ttctatttgg attgtcaaaa gaaacaggtt atatacgagg ttgtggatat gttgcaactt
    58201 ttgcatcttt tttcttttat gcaatatatt cagcattttt atcttttaaa tatagaaaag
    58261 caatgcatga atcaaaaata tgggctatta gaggttttct tatagtttca attgtggcaa
    58321 ttatcttaca ggcaattttt ttacagtatc ttcttacagg aactgcatgt gcatgtagtt
    58381 tacttttgac atatctttct atgcagaatc gaggtcttat tattgatgac cttactggtg
    58441 tactcaatag acaatctttt attcaagagc tagatttgaa tatcaatact gaacaatcag
    58501 gatttattat tacaattgcg cttgatgatt ttaagtttat gaatgaaact tttggaacaa
    58561 aaaatggaga tattgcacta aaagaagttg gaaaatattt gattcagata catgatgtaa
    58621 accatgtata tagaataggg ggagatatat tttctattgt tgtcaatagt caattaggta
    58681 tagaaccaga tgatattatc agaaagatag aagaaagatt taaaaagcct tggctagtag
    58741 aagagataag ttttaattta gcaactagta ttgctgttgt ttattaccca gaaaatgcag
    58801 aaacgacaga agatgtaatt gttgctatag atttctctat acatgaagca aaacgctctg
    58861 agtcaaggcg agttgtttat gcagatgcgt ctataagtga aaagataaag aagaaacatg
    58921 ttattaaaaa ttgtttaaaa gaagcattaa aaaatgatgg gtttgaagtg tactaccaac
    58981 ctatattttc taatagcagg ggcaggttta cttcagcaga ggcattactt aggctagaac
    59041 acaaagagtt aggttttata cctcctgatg aatttattcc aattgcagaa caaacaggat
    59101 tgattaactc aattggatta gtagtctttg agaaggtatg tagatttata gctagtgatg
    59161 agtttgaaga tttaaaattg gataatatag ctgtgaatct ttctgtagta caatgtatgc
    59221 aaaaaaactt agctgatgat ttactttatc ttatgaataa atataatgta tcaccaacta
    59281 aatttaaact tgaaattact gagactgtgg ctatgggctc ttttaatata attaaagaaa
    59341 caatggaaag attaattgat ttaggtgtta agtttgcact tgatgatttt ggaattgctt
    59401 attctggagt tactaatatg ctttctttac cattttcagt aattaagtta gataagagtt
    59461 tgatttggtc tatgaatgaa gatagtaggc ataaacttac agttgagacg attatagcac
    59521 ttataaataa attgaatatg aaggctgtag cagaaggtgt tgaaaccatt aagtgtgctg
    59581 aagatttaat agctatggag tgtgaatatt tgcaagggta ttatttttct aaaccagttc
    59641 cagaagatgt ttttaaaaat ttgttgaaag aaaatacttt tacaagttaa caccctttat
    59701 ttttttaatt acaactttgt tcattgacac tgccagcgat atattataaa attaacacaa
    59761 taataaatat taaatttatt atacatatta ttaatattaa ttaatggagt aaagatttat
    59821 tatatatttt atttaattat cctaaagtga gaaaggaaga tgtgacatga ataaaaagaa
    59881 aatattagtg ccaattgatg ggacagaaag aagtatgcat tctttggaat ttataaaagg
    59941 aatatttaaa aaagatgaag ttgaagtaga aataatgaat gtaaaagaac tagtatttat
    60001 tgatggcata tccctggctg aagagataaa aaattctgaa aatttaggaa gaagaatctt
    60061 ggataaagca gcagagataa tgggagagta tgatgttaaa gtacatttta cttttggata
    60121 tccaggagat gagataatta gaaaagcaaa agaagataat gtagatttta tagttatgac
    60181 aaaatctact aaaaaaggtc ttacaagaat gataggttca gtaacagcaa gtgtagttaa
    60241 acaagcaaaa tgtatagtga tgattgtacc ggagtagaat tagaaaggta tttgaaatat
    60301 gaataaaaag atagtagcag tttgtgaaag tgaaagaagt ttaaattctc ttaagacagc
    60361 tgcaaatgat ttaggatttg atttagttta tgaaattcaa gaaaatgata atataaaaaa
    60421 tgaactttct atttatgata tagaagatgc atccatagtg ttatttgtta caagtgattc
    60481 tatagaaagc atagagaaga ttgatagatt tatagataga gaattttatg aagttgatcc
    60541 aaagtatata atagaggatg caaaatctat agtaagtgaa attttaatag acttaaatta
    60601 atcgtgggtg tcgcactaag aatagagcga cgccttttta tatattttac acaagaaata
    60661 aagaggttgt atcaaaatat agataagagt ctttttcatt gttgaaatga tgaaaatata
    60721 tttgatttca tacaaaaaag agtacctcat aactaaaaag ttcactttga gacactccat
    60781 ttttatatgt tagttgcatt taaggtaaaa tagaagttta atcatgttga aataactgaa
    60841 ttacatcttc taaatgattg tacattgatg tataacctaa gtaagagtct ttactatcaa
    60901 atgcaatcat tatcttgtta tggtggctaa tcatatcctt gagagaattt ctatatttaa
    60961 aaccaaagta ttggttagaa gaaactgttt taggtattaa ctcataatat tttataaaaa
    61021 ttggattatg agaagctaaa attatctctt tgtgaaattg caagtcagca tcataacatt
    61081 cttctatcaa accatgagaa accttatcca attcctctat tgactttagg taagtatatt
    61141 ttattttttg aagttcttta gatgtagcat ttaaaattgc tagtttagta gcttcgcttt
    61201 cgataagttg tcttgctctt aaagattcta cataaaattc ttgtatgtta tgatttttat
    61261 agatttcacc atcatcaaat aggtcaactt ttttaaatcc ttcaactata ggacttgaat
    61321 aaggcacagc tctaagtatt ccttccttac agagtatatt tatggcattt cttacaggta
    61381 ttctacttat gttaaatagg tcagcaatct ttctctcact tggtaattta ctattaattt
    61441 taaaagttcc atttagaatg tttaatttaa tataatcaat aattttttca gtggcattta
    61501 agcttttcat aaaaactcct aaacatataa tttttttatg attgtattat atcatagtta
    61561 agagttaaat ctactttttt ataatatgta taagacagga tagaggccaa aatagctata
    61621 atagccaaga taaagtatcc atattgtatt gaagtatatt ccgatatttt accaactatc
    61681 attggaaata cagacattcc tatagcataa attgcttgaa aaaaacccat tgcagtagat
    61741 ttctgatgtt ttggtacatt tttcatagac tcgctagtaa ggtaagaaag gagtatacct
    61801 gtagatatac ctggtagtac ttgtagtaca aatattatat taatattttt tattgttgga
    61861 actaaaaagc aataggctga aactaaaata aaaactaaag gaatccaaaa ttttggtcca
    61921 tacttagtac aaaatttagt agaagcaaag ccagaagaag caacagcaca caccatgtaa
    61981 aatatagaag atagacctat gagggtagaa ctagaaccta agtttttaag aatttgagta
    62041 gtaaatgaca ttgttgtaga catttgaata ccctgttgaa ttaatgccat aaaggaaaaa
    62101 aatacaagtc ttttatcctt gcagatagat aaatattttt taattgatat tttttcaggt
    62161 atttttttag aattatgatt ttttatattt aaggctagaa tcgttccaat aactgaagac
    62221 aatacactca tgatacataa ggctgtcatt cctaattttt cataaaataa agtactcata
    62281 acaaatccaa tcaacattcc tagattgttt accaatatta tcgtacttgt agctttttgt
    62341 tgatgttctg tagaaaaaaa gttggtatag tgaaccatgt aagaaatcca agttgatgat
    62401 gatattccag ataaaatgtt agctataaga aaaccattgc ctccaggaga aaagactctt
    62461 ataagagatg ctaaaccagc aaagaaaatt ccagacagta taaatatttt atgtttattt
    62521 atggaatcag caaatactcc aacaggaagc cttaataaac attgtgatat tccatatgca
    62581 cctattatta taccaatcat atttgtagat acattacatg cacttaaata tggattttgg
    62641 aaaggtatat atacatattg tgaaaaccag aaaaatgtaa caatagaaag taaaagtaat
    62701 ttttgattag atattttttt catattatcc tccatgtggt atttttataa tgcaaataat
    62761 ataccattta gattttttaa tcaagtactt tctctattaa aaaaaattta aatatataat
    62821 tattatttac aaaaaaatta tactttatag ttgaattaag aaaacgctta tgatactata
    62881 aagttaacaa atttgaatat ataacagtag acgtgttact ggtaatgcag gcaagatcga
    62941 atcaattttt atagattatc tatagagtac tatgggtata atctatttat ttggttcgat
    63001 ttttttgttt aattggccat aaaacaaaaa gcctatagtt gaaaatggag gattttttat
    63061 ggattataaa aatatagctc aagaaattct actaaatgta ggaggaaaag agaatgtaaa
    63121 tgaagtgact cattgtatga caagacttag atttaaagtc aaaagcgctt ctaaagtgaa
    63181 taaggataaa ttatctaaaa ctgaaggagt aataactgtt gttgagagta tgggtcaaat
    63241 acaagttgta attggaaata aagtaaaaaa agtatatgat gaggtcataa aaattgtacc
    63301 tcaatcaaat aacataggta aaaagaatga aagtcaagaa aagcaaggta taattaattc
    63361 tatattatca gcagtagctg gtatatttac acctacaata cctgctatag caggggttgg
    63421 tatgattaaa ggtattttat cagttcttgc tatgtattat atgaataaaa atggagtaga
    63481 tataaaagaa acgcaaagct atattatctt aaatgctatg gctgattcta tattctattt
    63541 tatgcctatt atactaggat atactgcggc taaagtattt aatgcaaata aaataatatc
    63601 tatggtatta ggagctacac tttgttatcc aacttttact gctttgatgg caggtgaaga
    63661 atctgttaga tttttaggat tggcagtaac aaaagctacg tatacatcat ctgttattcc
    63721 aataattatt gctatatggg ctctttcata cgttgaaaaa gtattagaaa agtatatacc
    63781 tgaggtaatt aaaataataa tggttccaac tttatcattg gtagtaatgt tacctgcaac
    63841 attattttta tttggaccaa tcggaattta tataggaaat gtcataaact tttcttataa
    63901 gtatatttat gaattaagcc cagcattgtg tggagcattt gtaggtgggt tatggtgtgt
    63961 gttagttata tttggtgctc atagagcatt attaccaata ggtataagtg atgttgctca
    64021 aacaggaaga caaaatttat tggcgtttgc aggagctgca aacttttcac aagcaggggc
    64081 tgctctagga gtattcttta aaactaaaaa tcaaggacta aaaacaattt ctatgtctgc
    64141 aactataaca gctttatttg gtataactga acctgcaata tacggtgcca atcttagatt
    64201 aaaaaaacct atggtatgtg ccgtaatatg tggtgcattt ggaggtgcga taatgggaat
    64261 gggtggagct tatggtaatg catttgcaaa ccaaggtgtt ttaactatac ctgtatatgc
    64321 tgaagcaggt gcattaggtt tcttaagtta tctaggtggt tgtgctatag cattttttgg
    64381 ttcagctatt tcaacttatc tagttggatt tgaagatttg gaagagtctt ctaataagga
    64441 aaattcatct ataaaagtag aaacgaagga tggtgttatt gatataactt cacctgtaga
    64501 gggcgaatgt atagaattgt cagaagtaaa ggatgatgta tttgcttcaa aagctatggg
    64561 agaaggtata gctgtgttac ctacaaaagg agtaataaca gcccctactg attgtgaagt
    64621 agcttcttta tttccaacat tacatgcaat aggattaaaa ttagataatg gagcagaaat
    64681 gttaatacat gttggaatca atacagtaga gttaaatggt aaatacttta caaaacatgt
    64741 taatcaagga gatttagtga aaaaaggtga taaacttata tcgtttgata tagataaaat
    64801 aaaaaaggct ggttatgatg taactacgcc agttatagta aacaatacat ttgattttgg
    64861 gcaagtagtt tcttgtaaat ctagttatgt ttcaaccaat gataatataa tttcattagt
    64921 attagctggt aattaagtta atttgactag agataagtaa attgatttaa aaggaaataa
    64981 gtaaatcaac aattaaatac atctaaaagt aagtttattg caatgaattg ttaaagaaaa
    65041 taattaatat ggagtaaatc tattgcaatg aagtaacaaa gaaaataatt aatatagagt
    65101 aagtttattg taatgaattg ttaaagaaaa gagttaatat agagtaagtt tattgtaatg
    65161 aattgttaaa gaaaataatt aatatagggt aaatctatct caatgaaata acaaagaaaa
    65221 gagttaatat agagtgaact tattgcaatg aatcaacaaa gaaaataatc aatataaaaa
    65281 ggagaaatat ttatgaatac aggatttcca aaagactttt tatggggagc atcatcttct
    65341 gcatttcaag tggaaggagc ttgggataaa gacaataaag gaaaaaccgt agccgactat
    65401 aattctttta aaaagtctca tttacaggca gatactaaag tcgcatcaga cttttaccat
    65461 aattatgaag aagatattga attaatgaaa gaattaggga tgaaaacata tcgtttttca
    65521 atttcatggg caagaataat accagatggt gaaggagaga taaaccagaa aggtttagaa
    65581 ttttataata agataataga caaactgata gagtgtgata ttgagccatt cgtaacttta
    65641 taccattttg accttccatt taaattagta gagaaatata atggttggga aagtagagaa
    65701 actgtctatg cttttgaaag atttgctaaa atatgcttta agcattttgg agatagggta
    65761 aagtattggc aacctcataa tgaacagaat ttaattgtta gagtggagga gagaattaat
    65821 atatatgatg aaacagattc atggaagata gataaaatgc gtgctcagat ggattataat
    65881 ctttgcttag cacatgcatt agcagtcaat gcatgtcatg agatgataaa agaaagtaaa
    65941 attggagcag cagtatcatc atctgtaaca tatcctctta cttctaagcc agaagatgta
    66001 tatgcagcta gaatgaatga taattttaaa gtgtactata tgcttgatat gcatcattat
    66061 ggagaatatc cagggtatta tatgaagtat cttgaaaaaa gaaatatagt tcctcacatg
    66121 gaagatgggg ataaagagat acttaaaaaa gctaaaatgg attttatagc tgtaaattat
    66181 tataggacaa attgtgcaga agctttacct gaagattcac aacatccatt tggattaaga
    66241 gaaggaactg ttgattttag tatgtatgga ctatttaaaa tgtcaatgaa tcctaactta
    66301 gaggcttctg agtatggagc agcaatagac ccatcaggtc ttagagttgc cttaaatgaa
    66361 tattggcaaa gatatcactt acctgttata ataacagaaa atggtctagg agctaaagat
    66421 atattagaag atggaaaaat tcatgatgac tatcgtatag actatttaag aagtcatata
    66481 aatgcatgta aactagctat tgaagatggt gtagagatga ttggatattg tccatggtca
    66541 tttacagacc ttttaagttc ttctcaaggt tttaataagc gttatggact tgtttatata
    66601 aatagaactg accatgaagt tttagattta aaaagaatca agaaagatag tttttactgg
    66661 tataaagaag ttatagagaa caatggaata gtaaaataaa cataaatata tattgagggt
    66721 tttagtatgt acattacaaa ggtattaaat aattcgttgc tacttgcaaa ggatgataat
    66781 gatgaagaaa taatattgat ggggaaaggc attggccata attataaggc cgggtctgag
    66841 ttaaataaaa aagatataga aaagattttt gttcttcatg atgaaaatat aaaaaagagt
    66901 attatacaat tggcaacaga aatagatgca gaatattttg gtatagctca aatgataatt
    66961 gcatatggaa tagaaaaata taacttaaaa cttatgaacc atatatattt ggccttaact
    67021 gaccacattg cctttgcagt gagacgtttt aaagaaggta taatgattga aaatcactat
    67081 ttgttcgaaa tcaaggaatt taatccaaaa gagtatgata taggaaaata tgctattact
    67141 gtttttaagg aggttcttgg tttagaccta ccagaagaag agattggtaa tatagcaggc
    67201 cattttataa atgcacaaca agataatcct tatagtgata gaaataagag aagtgctaaa
    67261 attgtaaatg ctattttgca aatagttcat tatcactttt ctattgtata taataaagag
    67321 tcattttatt ataggagatt tgtaatgcat ctaaaggcct tttcacaaag atttctggct
    67381 aatgaaccat caagagaaaa aatagatttt atatatgagc aagttcaaaa aaattgtaag
    67441 gaagagtatg aatgtgtgaa aaaggtagga gcttatatgt ataaggaata ctccagagaa
    67501 ttaccaagac aagaggagtt atatttgatg attcatattc ataagatttt aggagaatta
    67561 tgcgaaaata gttagaatat cttaatatgc tagtaatatt ataaaaaatt aaaaattaac
    67621 tgcactttaa atatgaataa ttgcattact taagggggtt gtcttaaaat tgattttttt
    67681 aattaaattt gaagctcaac tcctttttta aacctttata tcattacaac tttaatgttt
    67741 atacgactgt actttagatg tatatctact ttttacattt ctcaacatgc aaacctacca
    67801 ttttttgcag tatgttaatt gaaataaacc attgagttaa ccctaaattt attctggact
    67861 tataggaata gttccaaaga tactttaaaa tattttctgg aataattgct tatttcacat
    67921 atgttttatt gtgatgtttt gatttaataa attcttcaac tctgtatata gtaaatgtat
    67981 tatcatgttt gttttattca agcacactta taatacgctt gattttttca caatgatgaa
    68041 atatactaat gggtgttata ggtataagaa tcgcattaca cttgaagaag taagtgataa
    68101 atgaggtata aatcctcata tgataaacta ctatttcatt gaggaacata ttctaagtga
    68161 tgtcaagata ctacaatatg actaaattcc tagtgataaa aaagtctacg aatgagtgta
    68221 gaaaggggaa ctaagatgaa taaagtttta attatagatg atgataaaga tctttgtatt
    68281 ttgttaaaaa agagtatttc aattgaacag attgaagcaa actattgcca ttctgggaga
    68341 gatggactgg ttgaactttc aaaacaagat tatcagttag tagttctaga tgtgatgatg
    68401 ccaggtatgg atggttttga aactatggaa caaatacgaa aatatagcag tattcccatc
    68461 ttaatgctga catcaaaaag cgataattac agcaaagtgc atggattgcg agctggtgca
    68521 gatgactatc taaccaaacc atttgaaatt acagaattta ctgctcgtgt actgtcttta
    68581 atccgacgtt acacccgttt taatccaaaa aatgaggagc cacagcaact ttctttcaaa
    68641 ggtttgttga ttgaccttga ccagcataca gttactacca atggcgaacc tatgttgctt
    68701 ccacccaagg aatttggtgt acttctttac tgtgcaaggc atcagggtaa gattttgaca
    68761 aaacagcaga tttatgaagc cgtgtggggt gagccatatg tatatgatga cagcaatatc
    68821 atggctatta tcagtcgcct aagaaaaaaa attgaactag acccatcatc tcccatctat
    68881 ttgcaaacaa taaaaggaat tgggtatcaa ttcaacacgg aggtatgaaa atggatgaac
    68941 aaacgataat tcttttctta ctcttgctgt cgattggtct actttttctt tccatatttg
    69001 ctttttccaa gcttgggcat atttacaagc gcctaaaaga tatagaggag atactggctg
    69061 atgtacaaat tggagacgaa aatcgtaaaa tactaattaa gccatgtgat gttatggctc
    69121 cattggttta tcagcttaat gagattgtct acgattatga aaataagtta ctttctctga
    69181 aaaagtcgga taaagctagc aaacaactta tgacaagttt atctcatgat gttcgtacac
    69241 cattaacaac tctgattggt tatctggatg ctgtacacag tggtatagtt atcgaacaag
    69301 agcgggagga ataccttgaa atcgctaggc gtagagccta cgacctcaag gattacattg
    69361 atgttctttt tgactggttt cggttgaact ctgatgaatt tacgctgtca atagaatctg
    69421 tggaaattgc agagttgtca cgcaatattt tgaaagactg gattcctata ttccatgaaa
    69481 agaaactgaa ttttgaaatt gatattccag aaaatcgact aatggtaaac ctcgacccag
    69541 atggatattc ccgtgtggtc aataatctgg ttcaaaatgt tcttgctcac agtaaggctc
    69601 ggcaaatcaa gattaccatg agtgaggatt ccagaatggt attactccgt gttgaggatg
    69661 atggtgttgg gattgccaga gaaaatttac cacatatttt tgagcgactg tataagtgtg
    69721 ataaaggacg ttcagaaaaa ggaagtggtc ttggtctatc tattgtcagt caaatggtgg
    69781 agcgaatggg tggtcaggtt tccgtagaaa gtgagattgg aaagtatacc gtatttacag
    69841 tgagcttacc attaatataa aattgttttt cccccatgtg acatgtatac acatggggat
    69901 tttttgtaag ttataaagtt gcaaggttaa tacaaggttg ttgcaaggtt aatgtaagat
    69961 tgaggtggta tgataacttt aataaacaaa ggaggctttt attatttatg aataatttaa
    70021 ttattgaaac aaaaaatctc actaagcaat atggtgctca aaagagcgtt tctaatttga
    70081 atattcacgt tcagaaaggt cgcatttacg gattgctagg aagaaacggt gcgggtaaga
    70141 cgacaactat gaaaatgtta ttgggtctaa caaaaccaac tactggagaa attaaaatat
    70201 ttggtcagga cattcacaat aacgagaaga agcttcttcc tcgaattgga tgtttgattg
    70261 aatcaccagg gttttatcct aatctgacag gaacagaaaa tctcaaaatc tttgcaaggc
    70321 ttagaggaat accaaaaaaa aatgcagtag aatcagcact ggatgtagtc ggtttgccaa
    70381 acaatgacaa aaagttattt tctcaatact ctcttggtat gaaacagagg ttggcgattg
    70441 ctcttgccat tatgcatgac ccacaactgt tgattttgga cgagcctatc aatggacttg
    70501 atcctattgg tattgcagaa gtaagaacgt ttattcgtga tctctgcaca gaacgaggta
    70561 aaactatttt gatttccagt cacattcttt ctgaaattgc cttgttggca gatgacattg
    70621 gcattattga ccatggtatt ttgttggagg aagaaagtct tgcccaatta gaagaaaaaa
    70681 acagtaaata catacatttt gctgtatctg atgcagcaca ggcagcaagg cttctggaaa
    70741 cccaacttaa cctgaaaaat ttcaaagtag cagataatta caatattatg ctatatgata
    70801 cagatgtttc tgttgcagaa ataaatcgtg ctttcattct aaacaacatt gaggtatcag
    70861 aatctcattt atgcgaagat actcttgaag attacttcaa gaaagtaaca gggggtgaag
    70921 gaattgcttg atataatttc atgtgaattt tcaaaactca agcggttaaa atttatactg
    70981 atttctattt taggtgcttg cttatttcca attcccgcaa caattttgat tgcaaaggac
    71041 aacttgccct ttgagcaatt gttcaaatta gttgtcaatt ttggatattt tttgctatta
    71101 ccaattgtat taagtattgt tgctagccaa ctatttttta tagaaagaga taatgatgta
    71161 ttaaaaaatc ttgcaacagt gccagtgcaa aaaggaaaac tggctctggc aaaattggct
    71221 gtgttgctgt ttatatcatt gttctactct gttgcagggt taggagctac tatcattgga
    71281 ggtttcattg ttggaatagt agagggggtt gctgtaaaat taggaatgag cattgctttt
    71341 ggaattatgt tttttgttac tgtacttcct gttgtggtac tcattgtata tttcaatcgc
    71401 agctatattt tttctattat actttctttt gtatatgcta ttttcaattt tagtatatcc
    71461 ttaaatatta tcaattttga accaaataac ccaatattaa gtgtattacc tgctcctgtt
    71521 ataatgcgtt ggtggatggc attttgggga gatcccactg gcgaatacac ggcacttaga
    71581 cagccatatt tgctctcaac tcctgcttgt gtaggtattt tatgtttgat aaccattata
    71641 gcagtcctgc tgatttgtat gatatacaaa aagcaggaga attaggaggt atctatgctt
    71701 gatataatta ctacagaatt tcaaaaaata aaaagatata atattctatg gattggtgtt
    71761 gttgctgttc tgttttcagc actattggca gtttttcagg ttgtaagttc acacggcagt
    71821 gaccctctaa cgtatgagag ctttgcaaac ggagttatct ggaataattt cagtcttagt
    71881 tttccatttg ggattacact tattggagga tatcttatta accgtgaata tacagaccaa
    71941 accttaaaaa acatcctaac tgttcctatt tcattacgca aattgctagt gggaaaatta
    72001 attgcggttg gaggaattac tgtattgtta agtcttttca gcttcttatg cacatttatt
    72061 ttggggttgt tgttttgcaa tatcaatata tctgttacac tcgttgtaaa atccttagtt
    72121 cagattttgt ctgttaatct gtattgttat gttgctgttt tgccgattat tgcttatttt
    72181 ggtcgtaaac aaaatgcatt tctgacgggt gtagggattg catttgtata cggtttttgt
    72241 ggtgtgtttg ttgctggaag aaatctgaca gacttttatc caatcacagc aggtcttggt
    72301 ctagttggat acaacaatgg tgctggtact gtttatcagc cacttatcgg tgctatgact
    72361 ttgattgtaa ttctaatctt gacaactatt ctacttgtat ttacacctaa ttatgataaa
    72421 gttatgacca tatctaagaa aaaagggata aagcataaaa agtattctca taaataacat
    72481 aaatattgtt tctattattt aagttgctag acagtgaaaa gaaaaaatct gtcgtacagt
    72541 gtctatatgc tcatgtattc aagaagctac agttttgatt tttcataact gtagcttctt
    72601 tttgtattta ttaaatcacg actatcataa tacttgcaat aggtatagcg acaagagaga
    72661 gtactgtcat atgtgtttta tatattacat taagtatatt ctaaaatata tgttttaaat
    72721 aatatatttg aaaataggaa tatttaaata aaaaaaataa tattatatat agttgacaaa
    72781 aattggcaaa tgagataata tataaatata gaaatagtaa tcattactat tattaaaaag
    72841 ggaggaatta attatgaact taaaaggaac taaaacagaa aagaatttaa tggcaggatt
    72901 tgcaggagag tcagaagcaa gaaataaata tacatactac gcttcaaaag caagaaaaga
    72961 aggttataat caaatagctg ctatatttga agaaactgct aacaatgaaa aggaacatgc
    73021 taaattatgg tttaaacttt tacatgatgg tatgccttca acagaggaaa atttaaagga
    73081 tgcagcagca ggtgaaaact atgaatggac tgatatgtat gcaaaatttg ctaaagaagc
    73141 tagagaagaa ggatttgaca agatagctta tttatttgaa gctgttggaa aaatagagaa
    73201 agagcatgaa gaaagatact taaagctttt agaaaactta aatgaaggaa aaatatttaa
    73261 aagagatgaa gaagttgttt ggcaatgtca aaactgtggt catgtgtatg taggtactga
    73321 agcaccagaa aaatgcccag tatgtgacca tccaaaagcg tatttcaata taaaagctga
    73381 aaattattaa aaattatcat tattaaaaag gaatgttaac tatgaaattt tctaaacaac
    73441 gagaactgat tttaaatgaa atattaaata atccggttca tcctactgcg gattacctat
    73501 atgaaaactt aaaaaaagat aatccaaatt taagcttagg aactgtgtat agaaatttag
    73561 ctcagctaac ggaacatggc tttataagaa aggttagtat tccagggtat ccagatagat
    73621 ttgatggtcg aatagataat cactatcata tcatatgtga agtatgtgga gaggtatacg
    73681 atttagaatc cgaagttctt aataatttac aagaattaat atctgaggag acagacataa
    73741 aaataacatc ttataacata agttttaaag ggatttgtaa taattgtaaa aggtgtagcc
    73801 aagtaggtta gttttcaaat tctaaaaata attaatgagg tgagtttatg tgtagtgaac
    73861 aaaaattttt tatatgtgag acttgtggaa accttgtagg aatgatacaa agtggtggtg
    73921 tacctatttt ctgttgtgga aaacctatga aggaacttgt tcctaataca acagatgcag
    73981 cagttgaaaa acatgtacct gtaattgaag tggatggaaa taatgttact gttaaagtaa
    74041 gtagcacaac tcatccaatg actaaagaac atcatatagc atgggtgtat cttatgactg
    74101 aacaaggtgg tcaacgcaaa tgtctagcag tagatggaga acctgtagtt aaatttgctt
    74161 tgaatgatga tgataaagtg atttctgcct atgcttactg taatttacat ggattatgga
    74221 aggctgaact gtaatattta aataaattga gtcaaaaagg aggctataaa tgtattttca
    74281 tacaaatagc ctcctataaa tttatataaa ttcaagtttt aattttgttt aatgtcaata
    74341 taatataagg agaaaataat atgtcaattt ataaatgtag tgtttgtgga tatatttatg
    74401 atgaatctaa gaatgataaa acatgggatg aattaagtga agattgggaa tgccctgtat
    74461 gtacaaaagg tcgtagttat tttggaaaga taagtactgt ttactatgaa gaagatgaaa
    74521 agatagcaga agacatagtt gaagatgaat ctaaattaaa tactgaaaaa gagggagatt
    74581 taaattactt aagtacatat ttaagaagag atgatgaagt tgagaaacat atggatataa
    74641 ttcacgaaat ggctgttaca ggaaaatcta taatagaacc tatgagaact aaactaccag
    74701 taatttcttg ggatgatatt ttaattatgg gtgctcaatt aaatccatta cctttaaatg
    74761 agcatgatga agtaaataca acaacaataa taggaaaaaa agcaaagaaa cctatgatta
    74821 tagaaaatcc agtttatatt tcccacatgt catttggagc tttgtctaaa gaactaaaaa
    74881 tagctttagc aaagggtgca gcccaaaata agacagctat gtgtagtggt gaaggaggta
    74941 tattaccaga agagaaggaa gcttcataca agtatatttt tgagtatgta ccaaataaat
    75001 atagtgtaac tgaagaaaat cttaaaaatt ctgatgctat agagataaaa attggtcaag
    75061 gaacaaaacc tggtatggga ggtcatcttc ctggcgaaaa agtaactgaa gaaatagcaa
    75121 aagtaagaaa taagcctgta ggtcaagatg taataagtcc atcttgtttt gaggaaatac
    75181 aatccaaaga agatttaaag aaacttgtag atgaacttag agaagtttca gaagggcgtc
    75241 caataggtgt taaaatttca gcaggtcata ttgaaaaaga tatggaattt atagcttatg
    75301 ctaaacctga ttttgtaact attgatggtc gtggtggagc tactggagca agtccaaagt
    75361 tgcttaaaga tgcaacttca attccaacaa tatttgctct ttatcgtgct agaaaataca
    75421 tagatacaca tggattagat atagatttgg ttataacagg tgggcttcgt atatctactg
    75481 attttgctaa ggcaattgct atgggtgctg atgcagttgc tattgcaagt tctgctctta
    75541 tggcagcggc gtgtcaacaa tatcgtatat gtggttctgg taagtgtcct gtaggagtag
    75601 ctactcagga tgaggaattg agaaaaagat tacatataga aaacagtgct aatagagtgg
    75661 caaacttttt aaatgtaagt ttagaagaac ttaagacttt tgctagaatt tctggtcata
    75721 aagatataca tgatttaagt gttgatgatt tatatactgt taactctgaa atatcaaatt
    75781 atacaaacat acagcatgta tagattgaag tttgaaacta ttagatttta gatagctata
    75841 aaagtaaatg ataaatatat tttataaaag agagtgtctt aaaatgaact ttttagttca
    75901 taagtcactc ttttttttat gaagccaaat atatttatca ttttagcaat gaaaaagaat
    75961 cttatctctt tattttgaga tagactcctt ctatttatga aaattaaaga tattaaattg
    76021 taaaatattc tgtgatgtcg tactattaaa ctaaagattc tgtactttaa agtaaataaa
    76081 aatttaagga gtgattatta tggaaagatt gaagatacaa gttttagttg acaataatac
    76141 ttatattgat agatactttg ttggagaacc agcagtaagc tattatattg agattgatgg
    76201 aaataggatt ttgtttgact cagggtattc agatgtattc atatcaaacg ctgaaaaatt
    76261 aaatatagat ttaggaaatt taactcatgt tgttttttct catgggcata atgaccatac
    76321 aagaggtatc cagtttctag aaaataggta tgatttatct acagttgaac ttatttcaca
    76381 tcctaactgt tttataccta gaaaacatgg agagaaaagc ataggagctc cattttctgc
    76441 tgaggaaatt aaaaatattt ttatgtataa tcctaaggat aagccattta atctaagtaa
    76501 gaattgtgtg tttttaggtg aaataccttc tataaatgat tttgaaaaaa gagctaaaat
    76561 tggtaaatgt aaaataggag atttgtggga agatgattat gttttagatg attctgctat
    76621 agtttgtaaa actgataaag gtatttttat tgttacagga tgttctcata gtggaatttg
    76681 taatatagta gagtatgcta aaaaggtatg tggagatgat agagttattg gtatactagg
    76741 tggttttcat ctttttgaat tgaataatag gttagatagt acaatacaat atttggataa
    76801 agaagaaatg agaatgttgt acccttgtca ctgtgtttct tttaaggtaa aagcgaagat
    76861 gaatgaaata ttgaatataa atgaagttgg agtagggctg accataagta tatagtattt
    76921 gaaagtatat aagatttaag ttgaaggtta aaaagatatt ttctatagaa aatatctttt
    76981 ttgttacgtt tattttaaga gaaatcaata tacttttata tgaaatattg taaaaaaatt
    77041 tatttttaat gagaatatat tttcattaat aaacaatttt attatgtatt aataatgtta
    77101 gaataaatat aaataaatca aagttactta cattacagta tattttttca caaaatatat
    77161 tgatttattc ttttaaagtt tgataattcc aatgaatgta ataaaagaag gaattttata
    77221 ctatacgatt attattatat aataataaag aaaaaattat ataataataa agaaaaaatg
    77281 tataatattt aacattaaaa aagatatttc ataaaattaa aaaacaa
//
LOCUS       DAMJYX010000008        73045 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJYX010000008 DAMJYX010000000
VERSION     DAMJYX010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.008"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(72..845)
                     /locus_tag="PC479_000571"
     CDS             complement(72..845)
                     /locus_tag="PC479_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2803159.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(845..1231)
                     /locus_tag="PC479_000572"
     CDS             complement(845..1231)
                     /locus_tag="PC479_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803160.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1256..1573)
                     /locus_tag="PC479_000573"
     CDS             complement(1256..1573)
                     /locus_tag="PC479_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2803161.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1585..1845)
                     /locus_tag="PC479_000574"
     CDS             complement(1585..1845)
                     /locus_tag="PC479_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2803162.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1845..2141)
                     /locus_tag="PC479_000575"
     CDS             complement(1845..2141)
                     /locus_tag="PC479_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2803163.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="PC479_000576"
     CDS             complement(2210..4636)
                     /gene="gyrA"
                     /locus_tag="PC479_000576"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2803164.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="PC479_000577"
     CDS             complement(4656..6557)
                     /gene="gyrB"
                     /locus_tag="PC479_000577"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2803165.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6558..7673)
                     /gene="recF"
                     /locus_tag="PC479_000578"
     CDS             complement(6558..7673)
                     /gene="recF"
                     /locus_tag="PC479_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2803166.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="PC479_000579"
     CDS             complement(7691..7897)
                     /gene="yaaA"
                     /locus_tag="PC479_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2803167.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8034..9140)
                     /locus_tag="PC479_000580"
     CDS             complement(8034..9140)
                     /locus_tag="PC479_000580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2803168.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="PC479_000581"
     CDS             complement(9382..10704)
                     /gene="dnaA"
                     /locus_tag="PC479_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2803169.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11360..11497
                     /gene="rpmH"
                     /locus_tag="PC479_000582"
     CDS             11360..11497
                     /gene="rpmH"
                     /locus_tag="PC479_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2803170.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11575..11919
                     /gene="rnpA"
                     /locus_tag="PC479_000583"
     CDS             11575..11919
                     /gene="rnpA"
                     /locus_tag="PC479_000583"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2803171.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11951..12202
                     /gene="yidD"
                     /locus_tag="PC479_000584"
     CDS             11951..12202
                     /gene="yidD"
                     /locus_tag="PC479_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2803172.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12234..12941
                     /locus_tag="PC479_000585"
     CDS             12234..12941
                     /locus_tag="PC479_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2803173.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12910..13539
                     /locus_tag="PC479_000586"
     CDS             12910..13539
                     /locus_tag="PC479_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2803174.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13651..15030
                     /gene="mnmE"
                     /locus_tag="PC479_000587"
     CDS             13651..15030
                     /gene="mnmE"
                     /locus_tag="PC479_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2803175.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15128..17020
                     /gene="mnmG"
                     /locus_tag="PC479_000588"
     CDS             15128..17020
                     /gene="mnmG"
                     /locus_tag="PC479_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2803176.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17013..17732
                     /gene="rsmG"
                     /locus_tag="PC479_000589"
     CDS             17013..17732
                     /gene="rsmG"
                     /locus_tag="PC479_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2803177.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17895..18680
                     /gene="noc"
                     /locus_tag="PC479_000590"
     CDS             17895..18680
                     /gene="noc"
                     /locus_tag="PC479_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2803178.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18815..19588
                     /locus_tag="PC479_000591"
     CDS             18815..19588
                     /locus_tag="PC479_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2803179.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19590..20453
                     /locus_tag="PC479_000592"
     CDS             19590..20453
                     /locus_tag="PC479_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2803180.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20486..21628
                     /locus_tag="PC479_000593"
     CDS             20486..21628
                     /locus_tag="PC479_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2803181.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21762..22337)
                     /locus_tag="PC479_000594"
     CDS             complement(21762..22337)
                     /locus_tag="PC479_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2803182.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22597..23889
                     /locus_tag="PC479_000595"
     CDS             22597..23889
                     /locus_tag="PC479_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2803183.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            23994..24596
                     /gene="yedF"
                     /locus_tag="PC479_000596"
     CDS             23994..24596
                     /gene="yedF"
                     /locus_tag="PC479_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2803184.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24669..24932
                     /locus_tag="PC479_000597"
     CDS             24669..24932
                     /locus_tag="PC479_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2803185.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24947..25132
                     /locus_tag="PC479_000598"
     CDS             24947..25132
                     /locus_tag="PC479_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2803186.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25235..26431
                     /locus_tag="PC479_000599"
     CDS             25235..26431
                     /locus_tag="PC479_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2803187.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26645..26923
                     /locus_tag="PC479_000600"
     CDS             26645..26923
                     /locus_tag="PC479_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2803188.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26960..27394
                     /gene="ssb"
                     /locus_tag="PC479_000601"
     CDS             26960..27394
                     /gene="ssb"
                     /locus_tag="PC479_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2803189.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27413..27640
                     /locus_tag="PC479_000602"
     CDS             27413..27640
                     /locus_tag="PC479_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2803190.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27783..28103
                     /locus_tag="PC479_000603"
     CDS             27783..28103
                     /locus_tag="PC479_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2803191.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28111..29067
                     /locus_tag="PC479_000604"
     CDS             28111..29067
                     /locus_tag="PC479_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2803192.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29078..31072
                     /locus_tag="PC479_000605"
     CDS             29078..31072
                     /locus_tag="PC479_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2803193.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31073..31522
                     /locus_tag="PC479_000606"
     CDS             31073..31522
                     /locus_tag="PC479_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2803194.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31541..32869
                     /gene="dnaB"
                     /locus_tag="PC479_000607"
     CDS             31541..32869
                     /gene="dnaB"
                     /locus_tag="PC479_000607"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2803195.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33074..33382
                     /locus_tag="PC479_000608"
     CDS             33074..33382
                     /locus_tag="PC479_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803196.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33464..34753
                     /locus_tag="PC479_000609"
     CDS             33464..34753
                     /locus_tag="PC479_000609"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2803197.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35079..35154
                     /locus_tag="PC479_000610"
     tRNA            35079..35154
                     /locus_tag="PC479_000610"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35112..35114,aa:Lys,seq:ttt)
     gene            35161..35235
                     /locus_tag="PC479_000611"
     tRNA            35161..35235
                     /locus_tag="PC479_000611"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35194..35196,aa:Glu,seq:ttc)
     gene            35240..35315
                     /locus_tag="PC479_000612"
     tRNA            35240..35315
                     /locus_tag="PC479_000612"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35273..35275,aa:Val,seq:tac)
     gene            35349..35423
                     /locus_tag="PC479_000613"
     tRNA            35349..35423
                     /locus_tag="PC479_000613"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35381..35383,aa:Thr,seq:tgt)
     gene            complement(35806..36798)
                     /locus_tag="PC479_000614"
     CDS             complement(35806..36798)
                     /locus_tag="PC479_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803198.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36807..37865)
                     /locus_tag="PC479_000615"
     CDS             complement(36807..37865)
                     /locus_tag="PC479_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2803199.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            37990..39453
                     /locus_tag="PC479_000616"
     CDS             37990..39453
                     /locus_tag="PC479_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2803200.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39555..40352
                     /locus_tag="PC479_000617"
     CDS             39555..40352
                     /locus_tag="PC479_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2803201.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40365..42467
                     /locus_tag="PC479_000618"
     CDS             40365..42467
                     /locus_tag="PC479_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2803202.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42535..43014
                     /gene="rlmH"
                     /locus_tag="PC479_000619"
     CDS             42535..43014
                     /gene="rlmH"
                     /locus_tag="PC479_000619"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2803203.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43163..43474
                     /locus_tag="PC479_000620"
     CDS             43163..43474
                     /locus_tag="PC479_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803204.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43522..43839
                     /locus_tag="PC479_000621"
     CDS             43522..43839
                     /locus_tag="PC479_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2803205.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44052..44753
                     /locus_tag="PC479_000622"
     CDS             44052..44753
                     /locus_tag="PC479_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2803206.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            44977..46278
                     /locus_tag="PC479_000623"
     CDS             44977..46278
                     /locus_tag="PC479_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2803207.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46415..47509
                     /locus_tag="PC479_000624"
     CDS             46415..47509
                     /locus_tag="PC479_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2803208.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47511..48644
                     /locus_tag="PC479_000625"
     CDS             47511..48644
                     /locus_tag="PC479_000625"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2803209.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48662..49771
                     /locus_tag="PC479_000626"
     CDS             48662..49771
                     /locus_tag="PC479_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2803210.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49836..50576
                     /locus_tag="PC479_000627"
     CDS             49836..50576
                     /locus_tag="PC479_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2803211.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50581..51573
                     /locus_tag="PC479_000628"
     CDS             50581..51573
                     /locus_tag="PC479_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2803212.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51761..52642)
                     /locus_tag="PC479_000629"
     CDS             complement(51761..52642)
                     /locus_tag="PC479_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2803213.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52915..54303
                     /locus_tag="PC479_000630"
     CDS             52915..54303
                     /locus_tag="PC479_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803214.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54331..55284
                     /locus_tag="PC479_000631"
     CDS             54331..55284
                     /locus_tag="PC479_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2803215.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55344..55568
                     /locus_tag="PC479_000632"
     CDS             55344..55568
                     /locus_tag="PC479_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803216.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55561..56073
                     /locus_tag="PC479_000633"
     CDS             55561..56073
                     /locus_tag="PC479_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2803217.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56105..56959
                     /locus_tag="PC479_000634"
     CDS             56105..56959
                     /locus_tag="PC479_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2803218.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57493..58287
                     /locus_tag="PC479_000635"
     CDS             57493..58287
                     /locus_tag="PC479_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2803219.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58391..59197
                     /locus_tag="PC479_000636"
     CDS             58391..59197
                     /locus_tag="PC479_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2803220.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59259..59780
                     /locus_tag="PC479_000637"
     CDS             59259..59780
                     /locus_tag="PC479_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2803221.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60195..62282
                     /locus_tag="PC479_000638"
     CDS             60195..62282
                     /locus_tag="PC479_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2803222.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62297..62584
                     /locus_tag="PC479_000639"
     CDS             62297..62584
                     /locus_tag="PC479_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803223.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62611..63882
                     /locus_tag="PC479_000640"
     CDS             62611..63882
                     /locus_tag="PC479_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2803224.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63940..64539
                     /locus_tag="PC479_000641"
     CDS             63940..64539
                     /locus_tag="PC479_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2803225.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            64544..65449
                     /gene="dapA"
                     /locus_tag="PC479_000642"
     CDS             64544..65449
                     /gene="dapA"
                     /locus_tag="PC479_000642"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2803226.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65555..66187
                     /locus_tag="PC479_000643"
     CDS             65555..66187
                     /locus_tag="PC479_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2803227.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            66664..69171
                     /locus_tag="PC479_000644"
     CDS             66664..69171
                     /locus_tag="PC479_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2803228.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69513..71675
                     /locus_tag="PC479_000645"
     CDS             69513..71675
                     /locus_tag="PC479_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2803229.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71680..72297
                     /locus_tag="PC479_000646"
     CDS             71680..72297
                     /locus_tag="PC479_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803230.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            72486..72830
                     /locus_tag="PC479_000647"
     CDS             72486..72830
                     /locus_tag="PC479_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2803231.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        27912 a         8677 c        12182 g        24274 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata ccatctttaa aacagtaggt caatactttt
       61 tttatatttt tttataaatt tttttcatat tcttttttca gcttctctac tacctttttc
      121 tctagtctag aaacagtcat ctgtgatatc cctagttctt tagctatact agattgtgtt
      181 ttatcaaaga aaaatctatc tttaaaaatt ttaacttcta attcatttaa agtttcaata
      241 aacttattaa taaaatcttc atactctata cttccaaagt taccttcttc ttttccaatc
      301 ttatcaataa gagttatatc tttatcctct gaatcatcat tacttgatga atccaaagac
      361 attggttgat atccataaga agcctccata gcctctaaaa catcttcttc actaacgcct
      421 atataatcag ctatatcttt tacctttgga tgcttcttat tttcttgttc taatttaatc
      481 ttcgcatcac ttatcttttt gcttaattcc tgtattcttc ttgggactct aagtgtccat
      541 accttatctc taaaatactt ttttatttct cctactatag ttggtgttgc aaagctggag
      601 aactcaaatc ctttctcaac atcatatcta tcaatagcat aaattaatgc caatgaagca
      661 acttgatata tatcttcaaa atcaacacct ttgtttatat acttcttagc taacaatctt
      721 gctaaataca gatgtctttc aataagaata tttctaatgt ctacattttt atttttatta
      781 tataaattaa atagctcttt tgtatccata tttaggtaat gtgtagcatt agctacattt
      841 ttcattaaat atcaactcct aaatatttag tcatttttat ttttgtccct tgattatgtt
      901 ctgattcaat atctacatca tccattaaag ttttaattat aaacaatcca agaccactct
      961 cttttggatt ctctaaatct ggtgtactta ttgagctaac atcatatcct tttccattat
     1021 cttgaatttc tatattaatt gcattatcta aaatattaaa tactatatta aacacatcat
     1081 ctttactgtg ttttattgca tttgtacatg cttcagatac tgctaccttt atatcttcta
     1141 tatcatctat tggaaaaccc atcttattgg caattccaga tgctgttaat cttattatac
     1201 taacataatc tggatttgta gtaatttcca tctttatagt ctcacaagcc aaagtttatc
     1261 cctccacttt aaatatttta tctagacctg ttatagtaaa aatctttctt acgttactct
     1321 taggatttac tatgtatatc tctttttcat ttatttttaa tttttttaat acacctatca
     1381 tagctcctaa gcctgttgag tctatataat ctaaatcttt aagattaatc tttacatcta
     1441 acatattttt ttctattaaa gcatgtaaat gttcctttag tttatctgct gtagaaacat
     1501 caagttctcc atcaagacaa acattccaaa atttattttg tgaatctaaa tttgaatcaa
     1561 tattcataga catgtaaaaa cctcctattt ccttttaata attttaaata tatttcctat
     1621 actcaatatt ttattaatta cataaactat atcatctatt ccctttgata ttgatgatgc
     1681 attttcaaca atatcttgta tatgtctttc attataatct acaatcttat atgccttttc
     1741 aacaacctta tttatgctgt ttagagtttt agctaaataa attgctacta taagagcaga
     1801 gcttcctact aacactgctc caatttgcca cccccatgca ttcattagtt aacatctccc
     1861 tcatcatcaa agcttttact tattactata tcttcttctg aaatctttat atcttcttca
     1921 ttattatcat catccactaa atttattatt ctctctttaa catcattaaa tgtttcatgt
     1981 agaacttctt ttggattttc tttgacatca ttaaacttct cttttgtttc ttttcttagt
     2041 tcagaacctt ttttaggtgc aaaaaacata ccaaatataa accctaaaat agctcctaat
     2101 aataaaaatc cacctttttt acaacttctt ttattgcaca taacaattac tcctttcaat
     2161 tttaaatttt tatctaacta cattatacaa aaagaatagg atatttatcc tattcttctt
     2221 caatatttat tttggcaatc gaaactacat tgacttcatc attcgtcttc attaatttaa
     2281 caccacttgt aactcttcca aacagtgata tttcattgac tcttattcta ataagaactc
     2341 catcagaatt tataatcatt atctcatcat cttcgttgac catatctgca cctacaattg
     2401 taccagtttt ttcagatata ttataagttt taattccttt accagctctt atttgacttc
     2461 tatactcttc tatatttgtt ctctttccaa aaccattctt acttacaact aacacatctg
     2521 tacctttact gcaaaggttc atagacacaa caaaatcttc tttacttaaa gttatacctt
     2581 ttacacccat tgctgttcta cccatatatc ttatatcatt ttcatcaaat cttatagaca
     2641 taccttcttt agttactaaa agtacttctt gttttccatc tgtaagttct actccaataa
     2701 gctcatcatc atctcttaaa cctattgcaa taagaccaga tttatttata tttttgaact
     2761 cttctctctt agtcttttta acaatacctt ttttcgttgc aagtaataaa tattcatttt
     2821 catcattacc atcaataggt attagagtag caattttctc atctgctgac aattgaagta
     2881 aattcactat agcagtacct ttagcctgtc tcttaccttc tggtatttca tatgcattta
     2941 atttaaatac tctaccttta tttgtaaaga ataatagtct actatgagta gttgtagtta
     3001 ccaagtgcct tacaaaatct tcttctctag ttgtaagtgc tgaaatacct cttccacccc
     3061 ttttttgact cttataagta tcagatggaa gcctttttat atatccaaag tgagtaagag
     3121 ttattgctat ttcttcatca cttataagat ctctcatatc tatttcgcct tcagcatgtc
     3181 ttatttctgt tcttctctca tcagagtaat tttcttttat tattgtaatt tcatccttta
     3241 ttacatttaa aagtaatctt tcatcagcta aaatctcttt taatctattt atcttcttga
     3301 ttaaatcttc atattcagct tctatcttat ctctttctaa acctgtaagt ctttgaagtc
     3361 tcatatctaa tatagcttgt gcttggattt cagttaattt gaatttttct attaaaccta
     3421 gcttagcttc ttgcccagtc tttgaagctc ttatcaagct tataacagca tctatattat
     3481 ctaaagcaat ctttaatcct tctaaaatat gtgctcttgc ttcagcttta tttagttcaa
     3541 atttagttct tcttgtaaca acatcttctt gatgtttaat ataatgatat aatatttgtt
     3601 taagatttaa aactcttggc tgaccatcta caagtgcaag cattattata ctaaaagtat
     3661 cttccatttg agaatgttta tacaaattat ttaatactat attagcatta gcatccctct
     3721 ttaattctat aacaattctc ataccatttc tattactttc gtctcttagg tctgatatac
     3781 cttctattct cttttcttta actaactctg ctattctttc aaccagttta gccttattta
     3841 cttgataagg tatttctgta actattattt gctgttttcc ttttggtagc tcttctataa
     3901 aagctctaga cctaacttta acttttcctc ttccagttct gtatgcttct gctatacttt
     3961 cttttcccat tataattgca gctgtaggga aatctggtcc ttgaacaaat tttattaaat
     4021 catctacact acactctgga ttatctatca aatatacagt tgcatcaatt acttctgcta
     4081 aattatgtgg aggtattgaa gttgccatac caacagctat accatttgag ccatttacta
     4141 ataaattagg atatctagct ggcaatactg aaggctcttt taacgactca tcaaagtttg
     4201 gtttaaagtc tacagtttcc ttttcaatat ctctcagtag ttctaatgat aatttactca
     4261 ttttagcttc tgtataacgc atagcagctg gtgaatcccc atcaacagaa ccaaagttac
     4321 catgaccatc tactaaaagt gctctagttg aaaaatcttg tgccattctt accatagcat
     4381 aataaacagc agtatctcca tgaggatggt acttacctaa aacgtcccca acaatacgag
     4441 ctgacttcct gtatggttta tctggagtta aatttaactc actcattgaa tataatattc
     4501 ttctatgaac tggctttaaa ccatctctaa catcaggaag agcacgtcca gctataacac
     4561 tcattgaata atcaatatac gattttttca tttcttccgc tatttcaata gggagtattt
     4621 tgttattttc ttccatgtat actaatcccc tctctctata tatctaagtt tctaacaaat
     4681 cttgcatttt cttctataaa ttcttttctt ggagcaacct tatcacccat aagcattgaa
     4741 aatacttcat ctgcaattgc agcatcttct acagtaactt gtaataaagt tcttgtctca
     4801 ggattcatag ttgtttccca taattgctct gcattcatct ctccaagacc tttgtacctt
     4861 tgaagttcaa caccatttct tccaatttca tctagtaaga tatttaactc tttatctgag
     4921 taaacataat gttccttctt ttgctttgtc actttatata aaggtggctg tgctgcataa
     4981 acatacccct catctataag aggtctcata tatctaaaga agaatgttaa taataaagtt
     5041 cttatatggg ctccgtctac atcagcatcg gtcataatta taattttatg atacctagcc
     5101 ttatctatat caaaatcttc tccaatacca caaccataag ctgttatcat attttttatt
     5161 tcatctgaag atagtatcct atctagtctt gatttctcaa cattaagtat tttccctctt
     5221 aatggaagta tagcttgact atttctatct ctaccttgtt tagctgaacc tcccgctgaa
     5281 tccccttcga ctaagaatat ttcactttta gatggatctt tttctgcaca atctgcaagt
     5341 tttccaggta aagatgtact ttctaataca ctttttctcc ttgttaattc tcttgctttt
     5401 tttgcagctt ctctagctct ttgtgctctt aaagctttat caactatgat tcttgctgtt
     5461 gatgggtttt cttctaggaa agaccccagt tcatcaactg ttacactatc aactatacct
     5521 ctcataaagc tattacctag ctttgtttta gtttgacctt caaattgagg ttctggtaat
     5581 ttaactgata ctactgctgt aagaccttct cttatgtctt caccaagtag gttaacatca
     5641 ttttccttta aaaatttatt tctcttagca taatcattta cagttttagt aagagctgct
     5701 ttaaatccac ttaaatgaga accaccttca tgtgtattta tattgtttgc aaaagaatat
     5761 atgttctctg tataaccatc tgtatattgc atagctagtt caacaaaaca atcatcaacc
     5821 tttttatcta tataaactat gtcatcatgt ataccagttc tagatttatt taaatactta
     5881 atatattcta ccaaaccacc tgtatagtga aattcttttc ttttttcttg tccttctctt
     5941 ttatcttcaa atactatttt tattccttta ttcaagaaag aaagttctct aagcctatac
     6001 tccaatgttt catatttaaa ctctatttca tcaaatattg tctcatctgg cataaattga
     6061 atcattgttc ctttatcttg agattctcca actacttcaa gcttagatgt tggcaatcct
     6121 ttctcatatg tctgcttata tatctttccg tttaaataaa cctctgctac catccatttt
     6181 gacaatgcat taactacaga aacaccaact ccatgaagac ctccagatac tttatatcct
     6241 ccgcctccaa actttcctcc tgcatgaaga ttagttaata ctgtttctaa agttgattta
     6301 ccagttttag gatgtatttc aacaggtata cctcttccgt tatcttttac taaaacactg
     6361 ccatcttcat taatagatac atatatgtct gaacaatatc cttgtaaagc ctcatctata
     6421 ctattatcta caacttcata aactaagtga tgtaaacctc tagggcttgt actaccaata
     6481 tacatacctg gtctttttct aacggcttct aatccctcta atacttgtat ctgacttgca
     6541 ccgtattctt gtttcattta atttcctcca ttctctatgc taataacatc gccattttca
     6601 atattaaata ttgtaacatt tttttcatca aatatttttt tatgtgaaac ttcagcactt
     6661 gttataaaca tttgaacatt gcttaaatta tttactaaaa gtttttgtct tgcttcatct
     6721 aactcactaa aaacatcatc taaaattaat actggatatt cttcaacttc attttttatt
     6781 aattctatct ctgataactt taatgatatt gaagcagttc tttgttgacc ttgtgaacca
     6841 aaaagtcttg catctaaatc atttatgaat atatttaagt catctttgtg tataccatat
     6901 ctggtagtct tagattctat atcattaggt ctattcgatg acaacttggc taaaaattta
     6961 ttatatactg tatctatagt atcttcatca gttatattta tttgattttt atatcgaatt
     7021 gataatcttt caactccatt agtcaaattc atatgcatat tttcagatat attagctatc
     7081 ttttttataa agtctctacg caaaatataa atataacttc catactttgc taacgtatca
     7141 tcatatacat ctaataaagc ttcatcaaca tgtatatttt taagcaccct actcctttga
     7201 gaaagtgttt tattataatt ggtaagatat ttataatatt taggtataat ctgactaatc
     7261 tctttgtcta taaatgccct tctttcttta ggaccctctt taaccaacct taaatcctca
     7321 ggagaaaata taacaacatt taagttgcca agtagttctt gtatcttttg taaaggaact
     7381 ttatttattc ttatcccttt tttatctttt ccaataatta gctctattag actatattta
     7441 ttatattttg aaaaacttcc tccaatatat aagttttcac tattaaatct aaccatttct
     7501 ttatctttgt tagtcctaaa tgattttcca aaagaaagca tataaataga ttctactata
     7561 tttgtctttc cttgaccatt tttaccaaca agtagattta ctttcccatt aaattccaga
     7621 tgcaactttt tataatttct aaaattaaca agttgcaaac ttttaagttt cacaaaaagt
     7681 tctccttttt ttacaatact tttattttag ttccttctat ttcaactata tctcctgatt
     7741 ttatcttttt tcctcttctt aattctattt catcatttac tgtcactaaa ccttcttgaa
     7801 ttaaaaattt tgcatgacct cctgttgatg caatctctgc taatttaaga aattgatcca
     7861 actttatata ttctgattct atagttattt cagtcataat agttcctcct tgtcactatt
     7921 ttaaacaaat tcctattgat acactaaaag tatattatac cataaatatg cttataaaag
     7981 gagttgtcta agattagaaa aattttctac aaaagacaac tccttcatgt ctgctatata
     8041 tttgatgata ttcttactgg aagcagtaag taaatataat taacatcatc tgttggttta
     8101 attatacaag gatttacatt tgtagtaaat tctataaata tttcctcatt atctatattt
     8161 ttcaaacctt cgataaaata tctagaattg aaagctatat ctaggtaatc tccatctaaa
     8221 tctatttcaa cttcttcata cacatttcct ttttctgtat tagaagtaat tgccattact
     8281 ttatctctta tagataactt tataagatta tttttttctg attgagacaa taaagacgct
     8341 ctctctatac tatttaaaag ttccttagta tttaatttaa ctctgctgtt atgttctctt
     8401 ggcaataatt ttttataatc aataaaatct ccttcaagca atcttgtaat aattttcgta
     8461 tcattaatta taaaaatagc atttttttca ttaaatccaa ctttaacatt atcctctcca
     8521 gaaagcaaac tatttacatc tataagtgtc tttccaggaa ttatgacttt tatattttca
     8581 gttaaactat caaccgaaca actttttaca gctaatctat atccatcaat tgctactaaa
     8641 tttaagtttc tatctacaat ttctaaaagt tctcccatta aaacaggttt tgtttggtcc
     8701 tgtgagatag caaatacagt ttgtttaatc atatttttta ggatttcttg aggtatgcta
     8761 tataaatctt cttcatttaa ttctggtaat ttaggaaatt cttttgctgc ataaccttta
     8821 attttaaatc ttgaatttac acaatttatg taaatattat tttctgaatc agtttctatc
     8881 tcaacaaagg aatctggtaa ttttctaatt atatctccaa atagccttgc atctacaaca
     8941 acatcacctt tttctatgat ttctgcttgt acataagttt ctattcctat ttcagcatca
     9001 tatccagtaa gttttaattg accttcttct gtagatataa gtataccttt taataattct
     9061 attgttgttt ttccattaat agctttttga gcaattccaa ttctatttgc taggattttt
     9121 tgattacaaa ttattttcaa tgtggataac ctcctctggc tttttatata taaaaataag
     9181 ttaaaatcat agtagtaata gtagtaggtg ttgttgataa tgttaataag tatgtataac
     9241 attataaata caaagatatt aacatgtgta taagatgtta atatctttgt tattttagac
     9301 ttgataattt gttaataatt tttgttcaaa aaaagtatta acaggttatt caagagatat
     9361 aaacatacaa ttgtgtataa attatccctt caaatctgat ataattttgt ctattttagt
     9421 cttaatttct tcactttctt ctatatcctt agatactttg tcatgagcat gaattactgt
     9481 tgtatggtct ctacccccaa actcttcacc tattttagga agggataaat cagtaagctc
     9541 gcgcgttaaa tacatagcta tctgtcttgg ataagctatt gaacgtgttc tttttttaga
     9601 attaaaatct tccatcttta aattgaatac agaagatact ttttctttta ttctgagaac
     9661 atttatttct tcatttttag aagttgttat aatatctttt aaagcttctg tagcaagatc
     9721 aaaagatatt accctattgc taagagaaga ataagcaacc actctagtta atgcaccttc
     9781 tagttccctt atgtttgact taatattttt tgctatatat gacataacct catttggcac
     9841 atctatacgt tctagttgag ccttttttct aagtatagca attcttgtct caaagtcagg
     9901 tgcttgaata tcagttataa gtcccatttc aaatctcgat cttagcctat cttctagtgt
     9961 aggtatgtct tttggcggtc tatcactaga tataattatt tgtttgtttg cttcatgaag
    10021 agtattgaat gtatggaaaa actcttcttg agttctttct tttcctgcta taaattgaat
    10081 atcgtcgatt agtaatacat ctacgtttct atatttattc ctaaattctt cgtttttatc
    10141 gtcttttata gagtttataa gttcattagt aaatttttct gaagaaacgt aaactacttt
    10201 tgagtctttt ttttgactaa caatatgatg acctatagca tgcattaggt gagtcttacc
    10261 taatccaact cctccataca aaaaaagtgg gttataagct ttagctggag attctgcaac
    10321 tgcaacgcat gcagcatgtg caaatctatt actattacca ataacgaatg tatcaaatgt
    10381 gtattttgga tttagattgg ggtaaagaac tctataattt aaatctgtgc tatcagaatt
    10441 taaatcagca acctcttttt cgctcaatac aaatttaata ttatattttt ttaatgacag
    10501 ttgactaata gcatcttcaa ttaaatgtaa atatctattc tctaagatat ctttattaaa
    10561 atcactagga gccagtaaga ttaaatcatt aagatgtatt tttaatggta cgatgttttt
    10621 aaaaaaggta ttaaaactta ctgaagttaa gtcacctttt attaattgta gggttttgtc
    10681 ccataaagaa actatatcca taatataaac ctccgaacag aataattaat attttccaca
    10741 aagttattaa cagaaaagca aaaatatata taaattttac agaataacta aatttatgga
    10801 taatggtgtt gataaattaa taatagtata aattattaaa aaaatatgaa aagatataaa
    10861 caatatgtag ataaagttat aaataacata aacatacaat aaatggtgat tataactaga
    10921 gtattgtcag taatcttaaa agttatacac aaaagtatcc acaagctgtg aataatttct
    10981 aataagttga taacatttat gcacattatc aacaagtata tttataatat caaaatatat
    11041 gtcaaacttc aatgatttgt aactaattta tccacaatat taactaattg ttgataaaaa
    11101 tataaacatg ttaaaattac tttatttgtg tatataattg aaaaaaataa tttagaataa
    11161 aattaaatag atttttacta gtattttcaa taaaaaaaat acataatata tatatggtag
    11221 attttgtgat ataagcgtcc caaaaactcg ttgacaacag tagtaaaagt atttataatt
    11281 atatgtgtta tgtttacttt gagggtaaag ttattatttt tataaacgga aaataaaatc
    11341 tgaaaggcgg tgcaaaataa tgagtaaaag aacttatcaa ccaaaaaaga gacaaagaag
    11401 taaagaacat ggtttcagaa aaagaatgaa gacgtctaac ggaagaaacg tgttaaagag
    11461 aagaagagct aaaggaagaa atagattaac tcattaatta caaaagggct gctgcaaagg
    11521 tggccttttt gtgtaaataa ttacaattca tagctgtgtt tatggaggtt aactatggac
    11581 tttaatagga ctaaagggtt gaaaaaagac tctgatttta gaaaagtata taaacacggc
    11641 aaatcttttg caaacaaata tttagtaata tatatactta aaaataaatc agattatagt
    11701 agagtaggta tttctgtttc aaaaaaagta ggaaaagcta ttactaggaa tagagtaaga
    11761 agattaataa aagaagccta cagattaaat attgatgaaa aaattaagcc tggatatgat
    11821 atagtattta tagcaagggt atctagtaag gatgctactt ttaaagacat agacaagtct
    11881 ataaaaaatt tagttaaaag aacggatatt tctatttaat tgtatgctat aaatatggag
    11941 gttaatgact ttgaataaaa taatttatat attaaaggaa ataagtaagt atttatcgaa
    12001 tttatgtatt tatttagtga gattttatca aaagtatata tctcccttaa agggacctac
    12061 ctgtagattt tatccaactt gctcacaata ttcaatagag gcgtttaaga aatatggatt
    12121 tgtaaaaggt atgtatttaa cgataagaag aattttaaaa tgtcatcctt ttcatccagg
    12181 aggttacgac cctctaaaat aaaagagagt aactattagg aggtataaaa aaattgaata
    12241 taataggaaa tttcttagga ttgattctta aggtaatatt tgaatttgtt aatcattatg
    12301 gtatttctat aatacttttt acgatacttg taaagattat tttattacca ttaacaatta
    12361 agcagactaa gtcaacaaag gcaatgcaag atatacaacc tcgtataaaa gagatacaag
    12421 aaaaatataa aaataagcca gaaaaacaaa atgaagaaat tgttaagtta tatggagaag
    12481 ctaaaattaa tccactatct ggttgtttac ctttattaat acagtttcct atattaatag
    12541 gattatttag cgttttgaga gaaccagttg ctcatggcgt ttttgcaaac aaagctgcat
    12601 ttttagcagc tgacaatggg tttttatgga taaaaagctt aacatcacca gattatgtat
    12661 tagcagtatt ttcaggagca agtgcatatg tcatgcaaaa agtaatgaca ccaaaagatc
    12721 agttacaagg ctctatgaaa gtaatgacat atgtaatggc aggtatgtct ttctattggg
    12781 gatttatatt cccagcagga cttacattgt actggacagt aagtaactta ttctcaatag
    12841 ctcaatatta cttaataatg aatcctttaa aagctaaact ggctgctaac tctaaggagg
    12901 aaaaagttaa tgagaaaaag cctaaaatta ataaaaagta agagtaaaga ggaagctata
    12961 agtaaagcta tagcagagtt gaatttaaaa gcagaggata tagaagtaga aatacttgaa
    13021 aatccaagta aagggttttt aggttttata ggtgctaaag atggtactta tgaaattttt
    13081 gttatcgaga gggaagtaga tgtagctaaa aattttatag aggtaatgtt gaaaaatgca
    13141 aatgttgatg ccaaagtcaa tgtatctcaa aaagataatt taatcatggt tgatattgaa
    13201 ggcaaagaag ctgcaagttt aattggtaga agaggggaaa ctttagattc tattcaattt
    13261 ttgactggat tagctcttaa taaaataaat aaagattctc atacaagagt acttgtagat
    13321 atagaaaatt atagatcaaa acgagaagaa tctttaataa gatatgctaa taaagttgct
    13381 agagaagtag cgaaaacaag aaaaactaaa aaattagatt atatgaatcc atatgagaga
    13441 agaataatac attcagctct tcaaaatgac aaatatgtaa ttacatatag tgaaggaacc
    13501 gatccatata gaagattagt aattgagtgt aaaagataaa gtcaacctcc taaacattag
    13561 ggggttttat tttttaatat tattaaatca ttaatgattg gttaaatgta aatctatatg
    13621 taaaaaatta tgaattaaga ggtgataaat ttgtttatag atgatacaat tgcagcaata
    13681 gctacagcac ctggagaagg tggtataggt atacttagaa taagtggaga aaaagctttg
    13741 aaggttgcag aagaaatctt taaatctatg tctggaaaga gtattgaaga atacaataag
    13801 agaacattaa tatatggaaa tatagtggat aatgaaaata taattgatga agttttattg
    13861 gcatatatga aaggaccaaa ttcctataca ggagaagatg ttatagaaat taattgccat
    13921 ggtggattta tttcggtaaa gaagatatta gaattaatac tatcaaaaga tgttagatta
    13981 gcagaggctg gtgagtttac taaaagagca ttcttaaatg gaagaataga tttatctcaa
    14041 gcagaggctg taattgatgt tataaaagcc aaaactgata tagctcatga agttgctcaa
    14101 aatcaattag aaggttcttt atcaaagaag ataagagaat tgagagataa agtcactgag
    14161 atattagctc atgtagaagt tgctatagat tatcctgagg aagatattga gcatataaca
    14221 tatcaaactc ttaaagaaaa aactgatgaa cttaaaaaag acattaaaaa attatatgat
    14281 acagctgaaa gtggaaaaat acttagagaa ggcttaaaga cggtaattgt tgggaaacca
    14341 aatgtaggta aatcttcact acttaattct attttaggag agaatagagc aatagttact
    14401 gatataccag gaactactag agatgttata gaagagtttg taaacataaa aggtatacct
    14461 ttaaaaattg ttgacacagc aggtataaga gatactgatg atattgttga aaaaataggt
    14521 gttgaaaagt ctaaagaatc atttactagt gcagatttga tagtaatggt tttagatgca
    14581 tctagaaagt tgtcagaaga agatatagag atattagaaa aacttaagga taaacaaaca
    14641 atagtgctgt taaataagaa tgatttgaaa caagagattg aagaagaaaa aatacttaaa
    14701 tatgtagaaa ataatagtat aataaaaata tcagctttac aacaagaagg aatagaagaa
    14761 ttgcaagata aaatagagtc tatggtatat aaaggtagta tcaaaaataa ttctagttta
    14821 gtagtaacaa attcaagaca taaagatgca cttagtaaag catataaatc tgcaacagat
    14881 gctttaattg ctttggaaca aagtatgcca tttgactttg tagaagtcga tttaaaaaat
    14941 atatgggact atcttggata tattaatggt gatacagtaa cagaggattt actagataat
    15001 atattccata atttctgtat tggtaagtaa tgtacaaaag taaacttgtt tttattatag
    15061 agatttttaa aaaatttgaa gtgtttctca tgaaacatta attttgaaaa gaaaggaatt
    15121 ataaatgata aagtttgaag caggaaaata tgatgttata gtagtcggag caggacatgc
    15181 tggatgtgaa gctgctttgg ctactgcaag aatgggatat aaaactttga taataacaat
    15241 gtctttggat tccatagcat taatgccttg taatccatca attggaggga ctggtaaagg
    15301 tcaattagtt aaagaaatag atgccttagg tggtcagatg ggattaaata tagacaagac
    15361 atatatacaa agtagaatgt taaatactgc caaaggtcca gcggtacact ctttaagagc
    15421 acaagcagat aaatttaaat atcatgaaga aatgaaaaag acattagaag atgaaccaaa
    15481 tcttgatata gctatggatg aagttgtaga aatacttcat gaaggaaatg ttgtaatagg
    15541 agttggaact aaactaggat gttcatttaa atctaaagct gtaatattgg ccactggagt
    15601 ttatctgaac tcaaagatat atatgggtga agttgcattt tatgaaggtc ctaatgcatt
    15661 ggggtatgca aaatatctta cagatagctt agttgagctt ggccttagaa tgagaagatt
    15721 taagacggga actccagcaa gagttcatag agatagtata gacttctctg taatgtcttt
    15781 acaagagggt gatgaaaaag taactccatt ctcatttatg aatgaaaata tagaaaaaaa
    15841 acaagaacct tgttatttaa caagaactac agaagaaact caaaaagtta tactggataa
    15901 tttaaaaagg tctgctatgt atagtggagt aatagaaagt actggtccaa gatactgtcc
    15961 ttctatagaa gataaagtag taagatttag tgataaaaca tcacatcaat tatttataga
    16021 accagaaggg ttaaatacta aagaaatgta tatacaagga atttcaacta gtttaccttt
    16081 tgaagttcaa ttagatatgt acaaaactat aaagggactt gaaaattgta agataatgag
    16141 acctgcatat gctattgaat atgattgtgt tgacccaact caactaaaaa taagtttgga
    16201 aataaagggt gttgagaact tatttagtgc agggcaattt aatggaactt ctggatacga
    16261 ggaagcagca gctcaagggc ttatggctgg tataaatgca gttagaaaga tagaaggaaa
    16321 agagccattt gtattagacc gttcagaagc ttatataggt gtcttattag atgaccttgt
    16381 tactaaagga actaacgagc cttacagaat gatgacatca agagcagaat atcgactata
    16441 tttaagacaa gataatgcag atatgagatt aactcaaaaa ggttatgata taggattagt
    16501 taaaaaagat agatatgaaa ggttcctaaa taaaaaggct gctgtagaga aagaatttga
    16561 aagactaaaa aatgaaagag ttacaccaaa agaagtaaat agtttgttag aagaaaaagg
    16621 agcaacacca ataaaggttg gaatatctct atatgaattt ttaaagagac cagaggttac
    16681 ttacgaattg cttgaagaat tagggaaagg cgctggagaa gatgtttcaa gagaagttaa
    16741 agaacagtgt gtaataataa ctaaatatga aggttatata gagaaacaat tgaaacaaat
    16801 agaccaattt aaaaaacttg agaataaaaa gctggatgag aaaataaatt attcatctat
    16861 agaaggtctt agattagagg ctagacagaa actagatgat ataaaaccta tttcaatagg
    16921 gcaagcatct cgtatatctg gtgtttctcc agctgatata tcagttttac ttatatacct
    16981 agaacaaatc agaagaacta gaggtggcaa aggtgaataa tatagaactt ctgaaaaacg
    17041 ggattgaagg ttttaatata gatacgaatg acagtatgtt agaaaaattt aaaatctata
    17101 gagagatatt agttgactgg aataaaaaga tgaatcttac tggaatagaa gatgaaaaag
    17161 aagtgtatat aaaacatttt ttagactcta tttcagctgt aaagaatgga tatataaaaa
    17221 atgggatgtc tataatagat gttggtacag gtgctggttt tcctggaata ccattgaaaa
    17281 tatgtttaga tagcttagaa cttactttgt tagattcgtt aaataaaaga ataaactttt
    17341 tagaggaagt tagtagagta ctagagttag ataatattac atttatacat ggaagagctg
    17401 aagattttgg taaagatgaa gactataggg aaaaatatga tgttgctacg gcaagagctg
    17461 ttgctggatt accgattctt atggaatttt gtgtaccatt tgtaaaagtt ggagggtatt
    17521 ttatttgctt aaaaggtccg aatgctaatt tagagctaga agaatcacaa aaggctattg
    17581 atgttttagg attggagtat attgaaaaaa tagatgtaga gttaccagag gtagatttaa
    17641 atcataatat attagttttt aaaaaagtga tggaaacacc agttaagtac cctagaaaag
    17701 ctggtaaacc agccaaaaat cctattaagt aatgaaaaat ttattattgg taagcttaat
    17761 tatttctcag gaaataatta agcttactgt aaaaaaacaa aaataaagga attattaaag
    17821 tagatgcaga aataaatttg tatgtacata tagagaacaa aaataattta tatctaaaat
    17881 aagagaggta tgttatggaa gataaaagag ttatggagat acctatagaa gatgtagttc
    17941 caaatccata tcaaccaaga aaaatatttt cacaagtttc attggaggaa ttgagcaatt
    18001 ctataaaggt atatggtata attcaaccta taacagttag agcaaaagat ggcaaatatg
    18061 aactaattgc aggagaaaga agattaagag cagctaaatt agcagagctt aaaaccatac
    18121 cagcaattat aaataatatg aacgatgaat cttctgctgt tctagcgtta ttagaaaatt
    18181 tgcaacgaga agacttaaat ttcattgaag aagcaattgg ttatgaaaac ttaataaaag
    18241 aacatgcttt tactcaacag caattagcag aaaaattggg caaaaatcaa tctactgtag
    18301 caaataagct tagaatatta aagcttccaa acgatataaa aatgaagcta atagagaata
    18361 atttaacaga aagacatgct agagcatttc ttaaactacc aagtgaagat ttaatgcaaa
    18421 gtgtgttaga caaagtcata aaaaatgaat tgactgttaa aaaaacagaa aaattgatac
    18481 aagatgtatt agaagaaact aaagttcaag aagagcctga taaaaaacag aatataaagg
    18541 gtgctatgag tataagaatt tacataaata ctataaagca ggcctttgat gctatatcaa
    18601 atacaggaat tgatgctaag tataatgaaa tagataaggg agattatatg gaagttgttg
    18661 taaaaatccc taaaaaatag agattactgc ttaccagtaa tctttttttg taatataagc
    18721 cttgtaatat aaattgattt atagacatta tttatactta aaaaacacct attcaacatt
    18781 taaaattaat ttaattctta aaggggggaa catcatgggc aaagttatag ctgtattcaa
    18841 tcaaaagggt ggagtcggga aaactacaac aaatgtaaat ttaagtgcta gcttaggaac
    18901 tttgggaaag aaaatattag tattagattt agacccacaa ggaaatacaa ctagtggata
    18961 tggtattaat aaaaacgaag tagaaaatac aatctatgaa attatgttag atgggttaca
    19021 tattaaagaa gcaataataa gtactgaatt tgagaatgta gatgtagttc catcagctac
    19081 tgaactatca ggtgcagaga tagagcttac atctaaaact aatagagagt atattttaaa
    19141 aaattccata aaagcggtaa tagatgaata tgattatata tttttagatt gtccaccctc
    19201 actggggatg cttacaataa attgtttaac tgctgtagat agtgttttaa ttccaataca
    19261 atgtgaatat tatgctttag aaggtgttag tcaattaatg gagactataa aattagtaaa
    19321 atcaagatta aatgctgata tagaaattca aggtgttgtt ttaagtatgt ttgatggaag
    19381 agctaattta tcaatacaag tagttgaaga agttaagaaa tactttaaag gtagtgtata
    19441 tacaacttta attccaagaa atgtaagact tgcagaagct ccaagtcatg gaaaacctgt
    19501 aatttattat gataaaagat gtagagggtc tgtagcttat ttggagctag cagaagaatt
    19561 tatagattta gaagaggagg aatggtagta tggagaataa gtctaaaaga agtaatagat
    19621 taggtagagg tcttagtgca ttgataccag aaattaaagg agaaacttca gaaaaagaaa
    19681 tagtcaacat agatatagac aaaatatatc caaacgaagt tcaacctaga aagcaatttg
    19741 atgaagaaaa gataaaagta ttgtcggatt ctataaaaaa ttatggtgtt ttacagccaa
    19801 tagtggttaa aatggatgaa aataataaat atatgataat agctggagaa agaagattta
    19861 gagcatcaaa acttgcaaat aaaaatcaaa tacctgctat aattaaagat atagatatga
    19921 aagatataat ggagatagcc ttaattgaaa acttacagag ggaagattta aattcaatag
    19981 aagaggctct agcatataag agtttaatag agcattacaa tgtaactcag gaagaaatat
    20041 cagaagctgt tgggaagagt agacctcata taactaatac actaagactt ttaaatcttg
    20101 gacaagatgt tatagaaatg atagacagtg gtagaatcac agcaggccat ggtaaggctt
    20161 tattgagaat tgcagataaa gatttgcaac ttcaaatagc gaaaaaaata gaagaagaag
    20221 aactttctgt aagagaagtc gaaaatatag ctaaaaaaat atctgaaaat aaacaagaag
    20281 aacctaaaaa atctaaacct aaggatgtat ttattttaga tgttgaagat aaacttagaa
    20341 atatatttgg aacaaaggtg aatatttcta aaggaaagaa aaaaggtaaa atagagatag
    20401 aatactataa tgatgatgat ttaaatagta ttgtatcaat gcttcttgaa tagaacaaaa
    20461 atataaaaca gagaggaaga ttgttatgat ttatttagat aatgctgcaa ctacatatcc
    20521 aaaaccagaa agagtgtata atgctgtttt agattgtatg aaaaattatt gtgcaaatcc
    20581 aggtagagca ggccataaac ttgctatgag agctgctagg gagatatatg atacaagaga
    20641 gaatatagct aagttgttca atgtaagtaa tcctatgaat atagtattta catctaatgc
    20701 aacagattct ttaaatttag cgataaaagg tgtattacaa gaaggagacc atgtaataac
    20761 aacgtctatg gagcataatt cagtaataag acctataaaa gcattggaaa aaaggggtat
    20821 tgaaaatact gtagtaaaat gcgattatga gggattttta gattatgaag atttagaaaa
    20881 atcaataaag agtaatacta aactgattgt aacaacacat gcgtctaatg tatgtggaac
    20941 tttaatagat ataaaaaaag ttggagaaat agctaaaaaa cataatatac tatttttagt
    21001 agatgcatca caaacagcag gtgtttatga tatagacgta aatgaatgta atatagatat
    21061 gttggcaatg ccaggtcata aatgcttatt tggacctcaa ggaacaggta tattatatgt
    21121 aagagaaggg ttgaacttaa atatattaaa agaaggtgga acaggtagta aatcagaaga
    21181 aatagttcaa ccagagttat tcccagataa atatgaatca ggaactcata atactccagg
    21241 tatagcaggt cttaaccaag gtatactatt tatatttgaa agaggaatca acaatataag
    21301 acaacatgaa gaagaattat gtcaatatat gatagacaaa ttagaggaag taccagatat
    21361 aaagatatat ggaccaaagg acagtaaaaa aagagcatca gtaatagctc taaatatagg
    21421 agatatggat tctggagagg ttactttctt attagatagt gattataata tagcgactag
    21481 atcaggaata cattgttcac cactagcaca taccacttta ggtactttaa aacaaggtgc
    21541 agtaaggttt agtataggat atttcaacac aaaagatgag atagataaag cagtggaagc
    21601 tttgaaaaaa atttcaaaaa ataaatagca tgagatttaa taatatagaa ataaaaaaag
    21661 gggatatttc aaaataaaaa tttgaatcat ctcctttttt gtacaaaaaa gaaaaggggt
    21721 tcctaagaac ctctaatcta tttgataata gtttcaagaa attactttaa gtaatctcct
    21781 tcttcaagaa ctatatgacc actttcgtaa ttagaaccat caacagtaat ttttatttgt
    21841 tcaacattca tattttttat gtaagtacga gcaactgaat caagcattaa agtttctgca
    21901 tcactaccta agtttgactt tgtaaagtca gagctaacat ctaatatacc agtttttaca
    21961 ccatcaatgt cttttgtagt aaatgaattt aattttgcag attctggaac aactttaagt
    22021 ttttgaagtt cttcaaataa agtgttctca tcaattttat ttaaatcaac tttattttca
    22081 attaaatctg tcttatctac atcaaaagag taaatagtag ctgattgagt tttagtttct
    22141 tcttttggag taggtttagc tttgttatta ggaacctctt tttcagtatt gttttcttct
    22201 tttggttgct caacatttgt attgctctta gtttcttttt tagatgaatc acttccattt
    22261 tgagcattag tacaaccaac tgcgaatata gatatagata atatacttaa cactaacatt
    22321 attttcttat ttttcataaa aatttctcct ctcttaaaaa ctattcttat atataattat
    22381 agtcgttatg agcaaatata tgaatacaaa atagtaacaa atttaaaaaa tgatttattt
    22441 aaattaataa aactcaaaat aaactaaatt ttagtcattt ttatgggaaa tcatttaatg
    22501 tacagtgaaa atattgtaaa ataaaagtag tgtttttaaa ataaatataa gttataatta
    22561 tattataaaa aaatgtcttc aaaaggtggg aatagtatgg aaattttgag cttaggagaa
    22621 aagattaaaa aattaagaaa agaaaaaaac atgacattaa aagaattagc tggagatagg
    22681 ataacagcag cacaaataag ccatatagag agggataaat ctcatactag ttatgaactg
    22741 ttggaatatc taggcgataa gctagatgta agtgtagaat acttattaga gaccaaggaa
    22801 atgcaatcaa aaaagataac tgataattta atactacaga gcgaaattta tataaaagaa
    22861 aatgaattaa acaaggctga ggaccaaata aaagaagttt tagatatatg taagagatac
    22921 gatttaatgg agaactatgg cagatgtaat tttttaatgg ctaatattaa tttgaaaaaa
    22981 tctaattata atgatgctat agaaaacttt gaaaaggcat tgtacttctt tataaaaaat
    23041 agtgatagtg aaaatatact taagtgttat ttaaacatag ggaaaatata tatgcaggaa
    23101 gacttttata aaggtgctat aagccacttt aattttgcag aggaaatttt gtcagagaat
    23161 aaatttgagg atgttaacat acacaaggaa ctatatagta agatggcata ttgttacata
    23221 aaactagata atccaaatat gtcattgaag tacatagaaa agataaatga aatagataat
    23281 agaaatgata gaaaagaaga tgtcgatatt ttggtgttaa aagcaaataa tatgcttgag
    23341 attggaaaat atgaagaatc aagagattac tttaaaaaag cactaaaaat acttgagagt
    23401 gaggataata aaactggatt agcaaatgtg tatttaacaa tttgtgatgt ttacagaaaa
    23461 ataggtgaaa cagatagagt acttgaatat tcacaaaagg tatatgacat aaagaaaaat
    23521 gatgaagatg aatacatgat gtcaggatta tttaagataa tagaagcata tatagataaa
    23581 gaagattatg atttagctag aaaatattgt aagatagcct tagcttcatc aataaaaaat
    23641 aaaaataaat tcaatgaata taaagcattg agattctatg ctaatatgta taaaaatcag
    23701 aatgaagtaa ctcttgcaat tgaatatctg attaaatgca taaaaatagt atcagattta
    23761 ggcaacaata gaatattggc caatttatat atagacttag gtcaattata ttcaaatata
    23821 tcaaaagaaa aagagttaga atattatcaa aaaggtgtat ttatgtacaa aaatttagaa
    23881 ataatgtagg gtaaataaac ctataagggt atagaaacaa tctataacaa gcaagataat
    23941 atctatgata aaatataaat tgtatgaata tttttaagga gatgaatcag acaatgagta
    24001 taaaaataga tgcgagaggg cttgcttgtc ctaaaccagt tataaataca aaaagagaat
    24061 tggataacct agaagaaggt gttgtagtta caatagtcga taatgaaaca gctaaggaga
    24121 atatattaaa acttgctaaa tctttaagtt gtgaagcaaa tattgttgac gaaaaagaga
    24181 attttatatc tatagaaatt agaaaaggta aaaatatact agataaacaa aatgttgtag
    24241 ataataaaga aagtgaatta gacaatacat gtatatttat atcttcagat aagatgggac
    24301 ttggaaatga cgaattagga aaagtgttaa taaaaggatt tatatataca ttaacagaat
    24361 ctaaacctta tcctaagtat gtacttttag ttaatggagg agtaaaatta agtgctgaaa
    24421 atgaagaaac catagaaaat ctaaaaatac ttgagaatat gggtgtagaa gttttatctt
    24481 gtggtacttg tttagattat tataatttaa aagacaaatt acaagtaggt acagttacaa
    24541 atatgtatac tatagttgag acattaaaga atgcatcaaa taccatttct atataatgta
    24601 tgtttgttta ataatataaa attaataata aagattaact caaatagata gtcagaaagg
    24661 attaagtaat gaatcaaatg tatatagtgt catttaattc gacacatcat gcgataagat
    24721 cagaaaagtt atttggtgaa aatagtctaa aagtaatggc tcttccaaca ccaagagaaa
    24781 taacagcaag ttgtggaata tcaattaaat tttcttttga agatatggaa aagataaaaa
    24841 ctattttagt tgaaaataat gtagatataa aaggaattta ttgtataagt aaattagaaa
    24901 atggctcaaa agaagtagag aagttagatt aggaggctaa ttatttatgc caatggattt
    24961 aaaaataggt gatattgttg aattaaaaaa gcaacatgca tgtgggtgta aagagtttga
    25021 aatcataaga acgggaatgg atataaagat aaaatgtact aaatgttcaa ggctaataat
    25081 gcttgataga gagaccttag aaaagagagt aaaaaagcta ataaaaaagt gataaaaatt
    25141 taacacttaa aaaatgtcaa ataccgtata ttaatatcca aaattttggt ataataagaa
    25201 ttgaagaaaa cattttcatg ggggtgcttt aattatggca gttaaatatg caaaaagaat
    25261 gcaaggatta caaggatcag aaatacgtga gcttttaaaa cttactcaac aaccacaaat
    25321 aatatctttt gctggtggaa tgccggctcc agagttattt ccagttgagg aaatgaaaaa
    25381 agtatcagtt gcagtgcttg aagaaaatgg aagatcagct atgcaatata ctacaacaga
    25441 aggatatgaa ccattaagag aaaaaatagc agctagaatg aatgataaaa acaaaactaa
    25501 tgtaaacaaa gatgatatat tagttacaag tggttctcaa caaggtctag attttgcagg
    25561 aaaagtattt attgatgagg gtgatgtaat tttatgtgaa agtccatctt acataggagc
    25621 tataaacgca tttaaatctt atcaacctaa attcatagat gttccaacag attctgatgg
    25681 aatgataatg gaagaattag aaaagatact agaaacaact gatagaataa aaatgattta
    25741 tgtaatacct gacttccaaa atccaacagg aagaacttgg ccacttgaaa gacgtaaaaa
    25801 attcatggaa atagtaaata aatttgaaat accagtaata gaagataatc catatggaga
    25861 tttaagattt gaaggagaga ctttaccatc tttaaaatca atggatacaa aaggattagt
    25921 aatattctta ggaactttct ctaaaatatt ctgcccagga tacagattag gatggacttg
    25981 tgcttctcca gaaattttat ctaagtttaa ctttgcaaaa caaggtgcag acttacaagc
    26041 atcaactata tctcaaatgg aagtaagcaa atttatggat atgtatgacc tagatgcaca
    26101 tgttgacaaa ataaaagcag tttatgttaa acgtagagat gtaatgctta agactatgga
    26161 agaagagttc ccagaaggat tagtatttac tcatccagaa ggtggattat ttacatgggt
    26221 agagcttcca agtaatttaa atgctaaaga attaatgcca aaatgtttag ataagaatgt
    26281 tgcttatgtt gctggaggag gattcttccc taatggtggt agagaaaata ctttcagact
    26341 taactattca aatatgccag aagaaaaaat aatagaaggt ataaaaaata tagcagctgt
    26401 attaaaagaa gctatgggtg tagaagctta attaaaaata ataacttata tataatacaa
    26461 gggatggttg ttgccatccc ttgtttgttt gaaaagtata taattcacct tatactaaac
    26521 tttgtccaaa atttaaattg acagaaataa gaataaatga taaaatgtgt aggtatgaga
    26581 ttttagtctc atttctctgc tctaagagtg tagagccgtt aagtccaaag ggaggtgaat
    26641 tatagtgaga aattatgaat tagtttatgt ggtaaagcca aactctgatg aagaagtaag
    26701 agaggctata ctaaacaaag ttaaggaagt tgttgcaact gacggagaaa tagttaaagt
    26761 tgatacttgg ggaactaaaa aattagctta tccaatagct aagtttacag aaggtttcta
    26821 tgtattagtt aacttcaaat cagcagtaga cgttcctaaa gagatagaca gaaacttaaa
    26881 gataaatgaa aacgtaataa gacacatgat agttgttgct taataactgg ttttattata
    26941 aaaaataggt ggtgttttta tgaatcaagt tgtattagtg ggaaggttga ctagagaccc
    27001 tgaattaaga tacatcccag gtacaggaac accagtggca tcctttacaa tagccattga
    27061 tagagactat gtaaaaaaag atggatctaa agaaactgat tttatacctg ttgaagtaat
    27121 gggtaaatct gctgaatttt gtgctaacta cataactaaa ggtagattag tagcacttca
    27181 aggatctata agagtagaca attatcagac acaatcaggt gaaaagagaa cttttacaaa
    27241 agtaagtact agatcagtac aagctttgga tagtaagaat aaatcggaaa attcatatag
    27301 agagagtgct ccagcttttg agccaagctt tgaacctcaa ggtttagacc cacaaggttt
    27361 ccaagctata gatgatgacg atataccatt ttaaaaagga gggaaaaaag tcatgatgaa
    27421 taaaaaaaga cgtaagaaaa aaagagtttg tcaattttgt gctgacaaaa acgctaaaat
    27481 agattacaaa agtactcaaa gattacaaaa gtatataact gaaagaggaa agatattacc
    27541 aagaagaata tctggaactt gtgctaagca tcaaagagaa ttaacagttg ctataaaaag
    27601 agcgagaaat atagcacttt taccatatac attagactaa aaaaatgagc cttgtgctca
    27661 ttttttatta tatgtattta tatatctaaa tgaggcgagc tataaagctc gtttttttgt
    27721 ttatatactt aaatatatta ttatttatga tataatatta tatattaagg agggttgtaa
    27781 acgtgaaaat agataaaggt tctgagataa cgagaaacat aaagataata gaatggatga
    27841 aaacagaaat attaatgagt gtaagtgatt tgtttaattt attgtttaaa ggggtaaaac
    27901 cattagatga agcaattcaa gatactttag caaatataat aatgataaca tatctcttag
    27961 caaagagatt aggaattagt tttagagatg tggattataa agtcaaggaa aaaataaaaa
    28021 ttggtataga tgaggaccat agcgttgaaa gatggtatgg agatttatct aatttaaaaa
    28081 aacatatgga taatagggag tgatattaaa ttgaataata aaattagact atctaaggca
    28141 atgtctataa tagttttagc tataataatc gctttagtaa tagcctatat tccaatgcta
    28201 ggaatattta gtatgttagc agctgttcca tatgtaatta taggtgctat tactgataga
    28261 aagtattctc ttatatctat tttggttaca ttttgtgtac tgattctatt tgcagacatt
    28321 tcttatgcat taaatatatg tataatgtat tctttaccag gtatagtaat aggaaagatg
    28381 ctaaagagaa gctttgaaca agaggacagt aataaattcg aaccgatata tggtggaacc
    28441 ataatatttg tactttcaat gcttgtatat ttttttgtat taaagacatt tttaaatgtg
    28501 aatctattag atgaagtttc taagatgata tcagaaattg taaatattca aaaaaatagt
    28561 ttttctgcaa ctgaacttaa agtgtttgat aaaatgaagc cagatgagat tgtaagttat
    28621 tttacaaata tgctaccaat gatgttattt ttacaagggt tgttatcagc gtttgtcaca
    28681 tattatttga gtgtattttt tataaaaagg attacaaaga tgaatataag atttcctaag
    28741 tttgctgatt tttatttacc gggaaatgct atactcacaa ctttcttact ttatttatta
    28801 gtcttattta ttcaaattat tggttcaaaa ttatatacag aattaataat gactaattta
    28861 cagctagttt ttaatttaat gtttgtaatt caaggtatag cagtttgcat ttatttctta
    28921 aagaaatgga taagacaggg accaaataaa attatgtttt tttcagggat aattttatgt
    28981 ttatttggat ttatgggaat atcttttgtt ggtatggtag atagtataat tgactttaga
    29041 aaggtaagaa gctataaatc cacttaggag gatagaaatg agtaataaac aaacctttaa
    29101 attaaatatg ccagaaataa atttatatat aattgttatt ggtatttcta gtataatttt
    29161 gctttattac aacttgtatg tagggtgttt atttttctgt atttttgtat atatggtttt
    29221 tcacaattgg agaactacta atattagacg tcatgaatgg acagagtata ttcaaaattt
    29281 atctttagat atagatgaaa caactaaaaa agccataata aatcttccaa taccattatg
    29341 tattttagaa tttgatggaa atatatcctg gtataatgga aagttttatg atatgattgg
    29401 tcaaaaagat ttacttgata aaaatataga ggatatagtt aaaaatctta atttaagaaa
    29461 agtattaaat gaaaataaag agatgtacac agaaataaac tataaagaaa aagaatatac
    29521 aataatttat aatgttataa aaaatgatca agaaaaaaat cctaaatatt tgatgatatt
    29581 atactggata gacaagactg aatatttaaa agtaaaacaa aattatgatg atgaaaaaaa
    29641 tgcaatgatg ctaatacaag tagatggata tgatgaagta ttaaaaagtg cagcagaaga
    29701 taagagagct ttaattaatg ttgaagtaga gaaaatactt tctgctttag aattaaattc
    29761 aaatggtgca ttgagaagaa cttcaaaaga caaattcttc ttggtaatgc ataaaaaaga
    29821 gttaaaaaaa ttggaagctg agaagttttc tattttagat acaataagac atattgatta
    29881 tggaaataat cttcctgtta ctataagtat aggaattggt atagatggag atacattaaa
    29941 tgagaaccta aaacttgcta cgggtgctct cgatttagca cttggtagag gtggtgacca
    30001 ggctgttgta aaaacaaaag ataaatttgt gttttatgga ggaaaatcta aagctgtaga
    30061 aaagaaaacc aaagtaaaat ccagattaat aggtcatgct ttaagagagg ttatacaaca
    30121 aagtgaccaa gtttatataa tgggacataa atatcctgat atggatgcta tgggagcagc
    30181 agttggtgtt tatgatatat gtaaatcctg taacaaaact gcaaacatag ttttacaatc
    30241 tgtaaatgaa tctatagaaa tatttataaa taaaataaat gaaaataatt actataaaaa
    30301 gttgtttatt ggaaaagagg aagctattga caactgcact aaaaatacat tagttgtagt
    30361 agttgatact catagaccaa attatactga atgtgaagaa ctgttaaagc tatcagagaa
    30421 ggtagtagtt atagaccatc atagaagagg tgtagagttt ataaatgatg cagtgctttt
    30481 attccatgag atatatgtat cctcaacgtg tgaaatggtt acagagttag ttcagtatat
    30541 ggatgaagat gtaacaataa ataaacttac agctgaaggg cttttagcag gtataagtct
    30601 agatactaaa aactttgcat tcaaaacagg tgttagaact tttgaagctg cttcttacct
    30661 tagaaaagtt ggagcagata ctatagaggt aaagaaattc ttcaattctg atgtaaaaga
    30721 tttcataata aaagctgaaa taattcaaag tactaaaata attaataata gaatatgttt
    30781 agcatattca agtactgaga tagatagtat aaatgttata atagctcaaa ctgctgatga
    30841 gttattaaat atcaaagaag tagaagcttc ctttgtatta ggtgaaaaag atgatactat
    30901 atttataagt gcgaggtctt taggacaaat aaatgtacat gtacttatgg aaaaattagg
    30961 aggcggggga cacatagata tagctggagc acagttaaaa aatgtttctc taaaagaagc
    31021 ttataagatg gtaaataaaa taatagagga gtatttggag gaggaagaat agatgaaagt
    31081 tatattatta aaagacgtaa aaggaactgg taaaaaggga gaaatgaaag aagtaagtga
    31141 tggatatgca agaaatttct tatttcctaa aaagatggct gtgcaagcag atagtgtagc
    31201 aataaaagaa ttaaatgaaa agaataaatc taaagaaata aaagcacaaa aagagtatga
    31261 agaagcagtt ttattaggta agcaaatgga agaaattaat atagaaatat attcaaaagc
    31321 aggagaaggt ggaagattat ttggttcaat aacttctaaa gaaattgctg agcaattaaa
    31381 gaaacaaaaa gacatagatg tagataaaag aaaaatatta ttagatgaac caataagatc
    31441 tttaggttca actttcgtag aaataaaaat acatcaaaaa gtaactacta aaataagagt
    31501 ggatgtaaaa gaaaaacagt aataactaga ggtgattaag atggaagata tgacgagaat
    31561 tcctcctcat agtgtagaat cagaacaatc aatattgggt tctatacttc tagataaaga
    31621 tgctataata acggttacag aaactataaa gcctgatgat ttctataaag aggctcacaa
    31681 aataatatat gaatgtatga taactttaag taataaaggt gagcctatag acttgataac
    31741 attaacagaa gaactaagaa aacaagggca tcttaatgat attggaggaa tcagttatat
    31801 tacaagtctt tctactatag taccgacgac ttcaaatgta aaatactatg ctgatatagt
    31861 aaaagaaaag tctgtactta gaaagcttat aaaagcttct aatgaaataa taaatctagg
    31921 atatagtgga gcaactaaga ttgaagatgt tttagaacaa gctgaaaaga gtatatttga
    31981 tatatcacag gagaaaacta gtgatgattt taaatctata aacttagttt tgatggatgc
    32041 ctatgatatg atagaaaaat tatacactaa caagagtgat gtcactggta taactactgg
    32101 ttttaaagat ttaaacaaaa aaataaatgg tcttcaaagg actgatttaa tattaattgc
    32161 agctagacca gcaatgggta aaactgcctt ttctttaaac ttggttcaaa atgcagcctt
    32221 aaaaggtgat gcctctgtag ctgtatttag tttagagatg tctaaggaac aattagtaca
    32281 acgtatgtta tcttcacaat ctagtgtaga attaaaaaaa ataaagactg gaacacttaa
    32341 tgataatgat tggcctagaa ttatagatgc tatggcagta ttatcagatg ccaaaataca
    32401 catagatgat actcctggta taaaaatatc agaactaagg tcaaagtgta gaaagttaaa
    32461 aatagaaaaa ggactagatt tagtgcttat tgattatctt caacttatgg aaggtgaagg
    32521 taataatgaa agcagacaac aagagatatc taagatatca aggtcactaa aaatattagc
    32581 aaaagaatta aactgtcctg tagtagcact atctcagtta tcacgtgcac cagaacaaag
    32641 agcagaccat agacctatgt tatctgattt aagagagtct ggagcaatag aacaggatgc
    32701 agatatagtt atgttcttat acagggacga atattatcat gctgattctg agagtaaaaa
    32761 tataggagaa gtaataatag caaaaaatag acacggtgaa actggttctg tggaacttgt
    32821 ttggcttggt gaagttcaaa gatttgggga taagttgaga gacctataat ctaacgaata
    32881 tccacagaac gtcctttctt tctacaggaa aaatgtggat acatgtcact aaatatgtcc
    32941 aaacattgaa atttagctaa aaaactatcc acactatcca cagatggcct gttgataagt
    33001 gtgaataagt agataaagca agcttttggc ttgctttatc tgtgtataaa tattaattat
    33061 taggaggaat aatatggaaa aaataagctt aaaatatata tatcccaata taattaaagt
    33121 acttgatgaa ataaacttat ttagagtaat tgacaataat ttaagagaat ctattgttgt
    33181 ttatgctaat aatgtggata atcagtatca tataaacatg actaatacaa actttggaaa
    33241 tataattaac atatgtaaat tagaaaaact tttagatgtg gataaattta tggaaaaagt
    33301 tataaaatat gaaaaagaaa taatagagaa agaagagttt tcaaaaatag aagaatatat
    33361 gttaaatatt ggagaatact aaaaaagttg aaaaaaatac gaatgtttga tagaataagt
    33421 aagtaaagtg ttcgagagta tataaaatat agaggtgaat tacatgaaaa cagttgcaat
    33481 tgttggttct caatggggag atgaaggtaa aggtaaagtt atagattatc ttgctactca
    33541 agcagatgta gtagtaagag ggcaaggtgg aaataatgca ggacatacat tagttgtaga
    33601 aggtaaaaaa tacgcattac atctaattcc atctggagtt ttaaatccaa atacagtgaa
    33661 tataatcgga aatggaatag tgtttgaccc taaaggattt ttagaagaat tggaaatgtt
    33721 taaaacggat aatataagta cagaaaatat aaaaataagt gatagagcac atgtcatatt
    33781 cccatatcat aaagaactag atgcattgtc agaagaagcc agaggcgatt taaagatagg
    33841 tacaacaaaa aaaggtattg gaccttgtta tatggataaa acagagagat ctggtattag
    33901 aatatgtgac ttaatggata aagataaatt tgctataaaa ttaaaagctc aaatagatgc
    33961 taaaaatgaa atagttaaaa atatatatgg aaaagaagaa ttatttgatt ttgaaactat
    34021 atataacgaa tatctaggat atgcagagca aataagaaaa tatgtggcag atacgtctgt
    34081 tattgtatat gatgcagtaa gagcaggtaa aaaagtattg tttgaaggag cacaagggac
    34141 tttattagat ttagatttag gaacttatcc atttgttaca tcttctcatc caacatctgg
    34201 tggatttgca attggtgcag gtatagggcc aaatatgata aaagacgttg ttggtatagt
    34261 aaaagcatat actacaagag ttggagaagg accgtttgtt acagagcaaa taaatgaaac
    34321 tggagataag ataagagaac aaggacatga atttggggta actactggta gaccaagaag
    34381 atgtgggtgg tttgatgcag ttatagttaa gtatgcagct agagttaatg gattgactag
    34441 tatttcattt atgttacttg atgtattaac tggatttgat aagataaaag tttgtacgtc
    34501 ttataaaatg ggcgataaga taataacgga tttcccagct tcattagatg atttagcaaa
    34561 atgtgaacct gtatatgaag aattagatgg atggaatgaa gatataactc aaatagataa
    34621 ctttgatgac cttccagaaa atgcaaagaa atatgtagca aaaatagaag aactagttgg
    34681 agtaagtgta gatatggttt ctgttggtcc aaatagagct caaactataa taagaagaaa
    34741 tatatttgct taatatttta tcaattaaat aatttaaaat agttgaagaa gagtatctca
    34801 agataatgtt tatagttatt agagatgctc ttttatattt gacttacatt gattttttat
    34861 taagttaaag ctacttatac atattctaac tgaaattaaa gttgaattta tgtagataca
    34921 tataggtttt ttaaaaagaa ttttgttgaa aataaatggc taaagatgat tttaattata
    34981 ttctaaaaaa agtaaaaaaa actattgact aaaaattata tagacgctat aatataactt
    35041 gtgagtgaaa gaaacgacga aaaaagtcga aaaaatgtgg tctattagct cagtcggtag
    35101 agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaaaatat
    35161 ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg cggtaacagg ggttcaattc
    35221 ccctacgagt caccagtatg ggactatagc tcagctggga gagcacctgc cttacaagca
    35281 gggggtcaca ggttcgagcc ctgttagtcc caccaaattt tgaattagag ataaatacta
    35341 gattatatgc tggtgtggct caacggtaga gcagctgact tgtaatcagc aggttgtagg
    35401 ttcgattcct atcaccagct ccacaaagta cataaattca aattaatata attttctatg
    35461 ttacaggata cttttaagta tcttttttta atttcatttt tattgtatta aaaatgaaat
    35521 taaacataca acaaaatttt gaattacttg caatgtgata gttttaaata taaaatttag
    35581 aatcattcta gcataatatt atgtaatata gcaaatttaa tttatagtat caataaatat
    35641 agactactaa gaatgtaaat gatttgttag attaaggtac attagatatg taaatagaaa
    35701 aataaaaagt atgttttaat ttatgtgata taagttttaa acagagaagc aagtttgctc
    35761 ttacctctct atttaaaaat aaaaaaatgt tttttaaggg ctattttatt taaatctttc
    35821 ccatctcaaa tcatctccag taaattctaa aatcctaaac ttatctaata ttctagagag
    35881 agttctttga gtgtaagtat ttcctatttg agatggtgtt aagttggttg aaataatgat
    35941 ttttttacca gcaacaagtc ttgtatttac aatattgaag atttctccac tagtaaaaga
    36001 attattaagt tcagttccaa ggtcatctat tattaataaa tcacaatcaa acaagttttt
    36061 ataattatct tcacttattt gattatttgt gtctcttcta aatttataat cttcaagaat
    36121 atctaaaatt ctaaaagaag tttgatagat tacaacattg cctttatcta acaattcctt
    36181 tgcaatacaa ttgcacatat aggtctttcc tagtccagta gaaccataaa acagtagatt
    36241 ttcactatta tccttcttaa aatcatgaac aaaattttcg cagatactta aattatttag
    36301 catattttct ctaggagata tttcaccatc agagcctttc ttaggagaaa atatattcaa
    36361 gttaaaattc gaaaaatttt cctgacttaa aatcctatct aaattagaca ttttataagc
    36421 ttcatttaca atttcttgtt tgaggcaaga acatttttct ccattaggta aaaatccttt
    36481 atctttacaa gaaatacatt gaaactttat ttccaagtaa tcaagaggta tgttactttc
    36541 agctaaaagt ctttctttct ttacttttaa agattctata ttctctttag tcttctttac
    36601 aatttcatct ttagacttag gatttaaaag tactatctta gctaaactga gacctatttt
    36661 agaaatttca tcatctatag attttatctc agggattcta ttatatacct cattttttct
    36721 atgttctaat aagagttcat tatcatctct tctcttagca tatttagcaa gtatttttct
    36781 tattttatcc tcattcattt aatcacctac ttaaactttt ctttttgact ttttttaata
    36841 atctcatcga gctcatcaga ggtatactga gtgaatgttt cgttgaaatt atgaaactta
    36901 gtttttttgt aagtattgtt gttttgagta gtattaatct gcttcttatt aatattctcc
    36961 ttaactatac gttcttcctc tttttgcttt aaatcattta aattttttat atttttttca
    37021 ttccaatttt taattatacc atttatataa gatatacttg ggtttgaaat attcttagac
    37081 ttagaacatg catttattat caaatctata tccatattaa atttatcaaa ccaggtgtcc
    37141 ataagttctt tttctgattt tgatggttgt cttgaaaatc ccaattcatt gaaaatcgtt
    37201 ttatataaaa tatatcgttc acttcttacc aaaaagcttt cttcaacatc ttttggcgta
    37261 tataaatttg aatcatacca atttctaata atgccttcaa tgtatttaac tggctttgat
    37321 gtaccagttt tatctttgac gtattcatat gcgcaaagta tcatatcggg gctcatattg
    37381 tatttattca ttatatccat aatactcatc ttttcacttg ggtcgagata tcgcccaact
    37441 attttattta tagaattaaa catttttcta atacttggat tttcagaagt agatacaact
    37501 ttatctgtat tagattttat agatgaatta ttgcttaata tattctcaat atagaaattt
    37561 tttaaatcta aaaattctat tgaataatta aaattatcat attcttcatt gtcatgtatt
    37621 tttataatct tcttttcttc ccagaatttc caagcagata acacatcaga caatggtata
    37681 tttaggtttt tggctattga attattgtcg aacttaggat tagaagtaat atcacatgct
    37741 tgtctatatc ctaaaagata aacttttacg tataacccgt ccgccattgg cataaatata
    37801 tctataaaga tattgggaat agtgatttca cctaaatcta tttcattgct ttctttaaaa
    37861 aacattttat cacctcagat taaattatat cattttttat attatatttg tctgtaaaca
    37921 aataaaaggt cacaagatta gcatgtaaat attttctaaa agtattcatt ttgaaaagga
    37981 ggagatattt tggttataaa attcaaccaa aaagtaagaa gaacattatc agtagcgatt
    38041 ctatttatgg tattaataat aggttgcttt atagaaaagg atgacagttt agaaacatac
    38101 aaagaaaaaa ctaattcaaa aataaatcag tataatatgg atgttatatt tgatgatgag
    38161 actaagaggt taatgtgcaa tcagaatgtt gactacataa ataatacaaa atcaaacata
    38221 gataaaatat atttccatat atatccgaat gctttttcca agaaagattt tgctcctttt
    38281 gaaaaaagtg aaatgacaag ggcttatcct aatggattta acgaaggata tatagatata
    38341 aagaatatat taaataataa tagtaaaatg gagtataaaa ttaaaggaga caaaaatgac
    38401 atattagaaa tagaattagg gaaagagtta aaaccaaatg aaagaatgtc attagatata
    38461 aaatataacg taaaaatacc aaattcaatt ggaagatttg gatatggaga aaatactata
    38521 aatgttacta attggtttcc tatagcttgt gtaaatgatg aaaggggatg gaatttaaag
    38581 agttatgaaa ctattggcga cccattttat agtgagacta gcgattttca tgtgaaacta
    38641 ttgattccaa ataaatataa aattgctcat acaggaaaac ttattgatga taaacatgat
    38701 aataagaaaa tgctatatga aatagaagct gaaaacgtta gagattttgc atttatacta
    38761 agtagtaaat ttgatgtaaa caaagtagat tcaaagggag tagctatcaa tacatataat
    38821 ttaaataaaa agctttctaa aaaagcaact gaagtggcaa aagattcaat aaatatattt
    38881 agtgaattat ttggaaagta tccttacaaa gaattttctg ttgtagcaag tgactttttt
    38941 atagggggga tggaataccc tatgcttgtt atgatagacc aaagtttata taatgaaaaa
    39001 aatgaatttt tactagaata tgttattgcc catgagacag cacatcaatg gtggtattct
    39061 gctgttggaa atgatgaaat aagtgaaccc tggttagatg aagcacttac tgaatattct
    39121 acaatagttt attttgaaca aaagtatggt aaagatatgg caaacaagct tataaaaact
    39181 atggagatac agacaaaaag ttatttgagt gaaaatatat ttaagccagc aaatgagtat
    39241 aaaaattcta ctgaatacag cttgaatgtt tatacaaaag gcgcaatagc atttaatgaa
    39301 ataagaaaag aagttggaga taaagtattc tttgagactt taaatgagta ctataataaa
    39361 tataagcata aaaatgctaa tggaagagct tttgtggaac tatggaatag taaaggtgta
    39421 gatataaata agattataag tgaatgtaag taaatagtga atgtaagtaa attttgtgca
    39481 gagtattaaa ttttatttaa tactctgttt ttttggtata atataattta taaggcagaa
    39541 aaagaggtga aggaatgcta aagtattgtt cagtagggag tggtagtagt ggtaattgcc
    39601 actatatagg gtataaaaat actaatatac tagtagatgc aggattaagt ggcaagagaa
    39661 taactacagg tcttaaagac attggtgtag atgcagataa attaaaagga atatttataa
    39721 cacatgagca tgttgaccat ataaaaggag caggtatatt atcaagaaag tttgatatac
    39781 cagtatttgc taatataaaa acatggtgtt ctatgaagga caaattaggt gacatcaaag
    39841 ataaaaatat gaaagtattt gaaaatgata aaacctactc attaggtgat atcatagtaa
    39901 gacctttttc aatagaacat gatgcgtctg acccagttgg atataatttt tatacagaga
    39961 gtgatgaaaa aatgtctata gctactgata taggatgtat aactcaaaat ataaaaaaac
    40021 atctatataa atctaaatta gtagtattgg aatcaaatta tgaccctaat atgcttatga
    40081 tgggttcata tacatatgct ttaaagaaaa gagtaatgtc aagtactggt catttatcca
    40141 atgaagatgc agctaatttc tgcgttgaac ttattaatga aggtacagaa tctatattat
    40201 tagcacattt aagcaaagaa aataattttc ctgagttagc gtatgagact tcaaaaggag
    40261 tattagcatc aaatgatata gtagttggac aagatgtaaa actagatgta ctatcacgaa
    40321 atgatgtttc taatgtttat gagatgaagt agaggtgaat aactttgcta aagagaaatt
    40381 ttgaaaatgt tgaggataat aaaattttat actctataaa gcagggtatg attttagcga
    40441 tacctgctat tatgactggt tcaactgctc ttgtaatttt gaatcttcca ataaaggctt
    40501 atcaagaata tttatcaggc ttgtttaatg gagaggttac aaatattctt aactttataa
    40561 atgattctac tttaggaatt atttcattaa taatattatt aacaataagc tatagttatg
    40621 gaaaaatata tgggagcaag tatacagtac ttgttcctat agttgcaatg tgctcttttt
    40681 tagtattttc acatggtaat gagcttaatt cttatataga aatatttaaa acaaagtggt
    40741 tatttacatc tataattgtt tctatgacat cttctgtatt gtttgtaaaa cttacagaat
    40801 cttttgttac aagtgtaaag tttcatacag aaggggctga tgcagacttt aatatggtag
    40861 tttcggctat agttcctttc ataatagtag tatttttgtt ttctattttt agagtggtaa
    40921 tgataagtct aattggcagt tcaaactttc aagatatatt ttataattta ttttcaaatg
    40981 tttttaataa aatgggaaca aaccttatga gtgctctgtt gtttattttt ttaatgcact
    41041 ttatgtggtt ttttgggatt catggtagta atgtattaga taccgtcgca aaaaatttat
    41101 ttgaaaatag tatggcaatt aacataaatt tagttaataa taatcaatta ccaacagaaa
    41161 tttttactaa gacctttttt gatacaatgg ttttattggg aggatgtggc tctttactat
    41221 gtttagtaat tgcaattttt cttagtgaaa aacgtataaa tgtaagaaag ttagctaaaa
    41281 ttgcttcaat accagctctt tttaatatca atgagatgct agttttcggg ataccaattg
    41341 tttttaacta tattatgttt gtaccttttg taattactcc tataatctta accgttacaa
    41401 gttatgtggc tatgtctaca ggtattgtac catgtacagt ttcagctgta gaatggacct
    41461 cacctatatt tttaagtgga tatatgtcta caggttcaat aagaggtagt ttgttgcaag
    41521 tatttaattt atgtgttggt gttatgattt atattccgtt tataaaaatg tcgcaaaata
    41581 gatatacata tatgtttaaa aataatatag aaaatttaac aaacttatta aaaaggtatg
    41641 aattaacagg tgaacagcct aatcttctaa accataatgg taaaataggt tcaatttcaa
    41701 aaatgttggc atcagacttg aagtttgcta tgaaaagagg agaaatagag cttttttatc
    41761 aaccacaagt caattataat ggaaatgttg tgggagcaga gggattactt agatggaaac
    41821 attcaatagg agggttcatt tatccaccac tagttataat tttggctaaa gaagaagggt
    41881 tcttagatga gcttggagag tatattatta ataaagcatg tattgattta aagaagagtg
    41941 aaaagctatt gagaaatcct gttaagttct ctgtaaatat atctccagac caacttgatg
    42001 accctaagtt accagagcaa ataaaaaata tcattagtag taatgatata aatcctaata
    42061 tgttaggtat agaaataaca gagcaaattg cattatcagg ttcacaaata attattgaac
    42121 gtatcaatgc gatacataac ttgggaataa agttaattat ggatgatttt gggatggggc
    42181 atagctctct tatatatctt caaaataata actttgatat agttaaattg gatggttcat
    42241 tagttaaaga gatacttaca aataaaagaa gtagtgaaat tataatgact attgtaaatc
    42301 tttcaaaaaa tttagatttt gatatagttg ttgagtatgt tgaaaatata gaccaaaggg
    42361 ataagttata tggtttagga tgtgaaatat atcaaggata ttattatagc aaagcaatac
    42421 cttttgaaga gtttgtagaa tatgcaaatc aagaaagaga gatataattt tataagagct
    42481 tgtaaattct agtaatgcat agatttgtaa taaaattaag tttaggagaa atatatgaat
    42541 ataagtatag tagtagtagg taaaattaaa gaaaaatatt taaaattagg tattgatgag
    42601 tttaagaaaa ggctttctaa gtattgtaag ttagaaatta tagagttgga tgatgaaaaa
    42661 gctccagaga atttgagtgt taaggaaatg gaaattataa aagacaaaga aggaaagaaa
    42721 attttaggga agataaagca taatagttat gttatagcct tggctattga tggtaagggt
    42781 ttatcctctg aagagcttgc taagactatg agtgatttag cagttagagg taatagtagt
    42841 ctctgtttta taataggagg ttctttaggg ctttctgatg aggttttagg aagggctgat
    42901 tataggttat ccttttctaa gatgactttt cctcatcaga tgatgagatt gatattgtta
    42961 gaacagattt atagggctta taggataaat aatggagaac cgtatcataa ataaatgata
    43021 cggtttttgt taatttgaca cttaataata ttaaaataac aaaaaagtat tgaaagtata
    43081 ttgcatttaa ggtagaatta aataaaatat tataacatta taatgtttat atggttgtta
    43141 ataaaaattt ggaggaaaat atatgataaa aataatgttg gcctgttcag caggaatgtc
    43201 tacaagttta ttagttacta aaatggaaag tgcagctaaa gaaaatggta tagagtcgca
    43261 aatttgggca ataccagaat caactataca aaatgaaata gaaaaatgtg atgtattatt
    43321 actaggacca caagttagat atgtattacc taaggctcaa gaaattgcta agccatataa
    43381 tataccagta gaagttataa atatgatgca ctatggaaca gttaatggag aagctgtttt
    43441 aaatagagcg atagaactta acaacactaa gtaatataaa ttataatgta tttttaatat
    43501 ataaattggg agaatcaaag gatggatgaa aagactatag aaatatcttt taatataata
    43561 ggttatgcag gtgaaggtaa aggattagct tttgaagcaa taaaagaggc taagaatgga
    43621 aatatagaaa aagcaaaaga attattaaaa gagtctaggg aagttataaa taaagcacat
    43681 agatatcaaa cagaattaat acaaaacgaa gctagtggaa acaggactga aataagtgtc
    43741 atacttatac atgcacagga ccacttaatg aatggcatga attttcaaca gttagctgag
    43801 gaaatcgttg atttacattt gaaattacaa gataaataaa aattaaagat aataaattaa
    43861 gagagttctt aaaagataca ttatgtgtag cacactaatt gttagattat tgattcaata
    43921 tttagtgagt aatacattag gattattttg aggactcttt ttatatttag tttaaagtta
    43981 ataaatattt attattaata atataagatt atatgttata atattataac atataattta
    44041 agaggtgaat catgttaaga aatcaaccat tatatataca agttgtagat atattaaagc
    44101 aaaaaatatt aaataacgaa tataaaattg atgaaaatat tccaacagaa agggagttag
    44161 agcaagagtt tggagtcagt aaaattacta ttagaaaagc tattgagata ttagaatcag
    44221 aaggatatgt agagaaaaaa agtggaaaag ggacaaaagt aataagtaat agccttttta
    44281 ataagctatc aaaagcagag tctttttcaa gtatactttt atctaaaggt cataagttat
    44341 ctaaagagac tatttcaatt gaaaaaataa taaatgatac aaattcacaa gtatttagta
    44401 tatttggaga aaaatgtagc aagattacta gaatgtatta tttagatagt aagccatata
    44461 tattttttac tcattatatt ccttttgatg aaaatctaac tgttgaaagt tttaataatg
    44521 aaagttcttt atatatgtac ttatatagga gaaatataaa tataactaga tttgaagatg
    44581 aattttttat agaagaatca gaactagagg tatcaaaggc tttaaatcta gctaaagaca
    44641 tattattagg aagaaaaaga aagacctatg atgagaatga taatatagtt gagctatcat
    44701 ttgcaagata taatacagaa gtacaaaact atgtaattaa atacgaaata taataagaat
    44761 cctgagttta ttgaaatgat aatatgctca ggatttttgt ttagttgtaa aaataaaaaa
    44821 tattataaca ttataatgtt tacaaccaaa cattataatg ttataatatt tttaataaac
    44881 aagtgatatt atcaattaca taaaaattag tttatgttat gaaatcgtta ttgcattata
    44941 aataaaggat aatatcaata atacaggggg ataactatgg aaaaattcgc aagtttttta
    45001 gagcgcaaga ttatgcctgt agcagcgaag ataagtaatc agagacatat gagagctgta
    45061 agagcaggta taatagcaac attaccacta acaatagtgg ggtcattttt cacaatatta
    45121 ttaaacatac ctattgatgc ttatatggat tttatagctc catttaaagc aatattagat
    45181 ataccattta gatatactgt aggattttta tctctatatg ctacttttgg gatagcatca
    45241 tcacttgcta aaagctacga tttagataca acaggtgttg gtatgcttgc agttatggca
    45301 tttttagttt ctacaatagt tccaactcaa gtagttgaac cagtaggtgg agttatagaa
    45361 gctggtagat ggatggatat ggcaaaactt aattcgccat cattatttgg agctatcgta
    45421 acatcattaa ttacagttga aatatatcgt tttatgaagg aaaagaatat tacaattaag
    45481 atgccagcag gagttccagt agaggtttca aattcattta ctgcattatt accgacagca
    45541 gtaattttaa tgttattctg ggtaataaga catattctag gatttgatat aagtgttgca
    45601 ctaagcgcta tgttatcacc tttaaaagga ttcttatcag gaaatagttt aggtggtggg
    45661 ttactgacag tattcctaat aactttattc tgggtacttg gaatacatgg tccagctata
    45721 atgggaccag taataagacc tatatgggac atgtctatag cagaaaatat aactgcattc
    45781 acagaaggag tatcagctca tgcaatgcca aacatattta cagaacaatt tttacagtgg
    45841 tatgtatgga tagggggagc aggggctact cttgcattag ttgtaatgtt tatgttttct
    45901 aagtcacaat atctaaaaag tttaggtaga ttaggatttt taccagggtt attcaacata
    45961 aatgaaccta taatatttgg agcaccaata gttatgaatc caatactagg gataccattt
    46021 ataattggac ctataataat gacaattcta tcatatgttc ttactattac aggggttata
    46081 cctatgatgg tagcaagatt accatttgca atgccagcac caatagcagc agtaatgagt
    46141 actaactgga gtatactagc aggtatctta gtattaatca actttgtgat atcatttgct
    46201 gtttattatc cattcttcaa agtttttgaa aaacaacaat tacaaagaga acaagaagaa
    46261 atgagtgaaa atttataaaa atacatagat atagaggttt tgtactatat aaaatataga
    46321 aaaagcttat aagttattaa gggtgaaggt aggaggaatg acctttgcct ttaattttat
    46381 atattttaaa taatgtaatt tgaggaggag aaagatggaa agaagattag gaatatcagt
    46441 atatccagag catagtaaca tggaagatga taaggcatat ataaaaatgg cttcaaaata
    46501 cggatttaaa agaatattta tgtgcatgct ttcaattaca agacctaagg aagaagttaa
    46561 aaaacatttt aaagagatta taacctatgc aagagaatta ggatttgaag taatccttga
    46621 tatagctcca aacatatttg atatattaca aatatcttat gatgatttaa cattttttag
    46681 tgagttaaat gctagtggaa taaggcttga ctatggatat gatggaagta aagaagctat
    46741 gatttctttc aatccatatg atttaaaagt ggaactaaat atgagtaatg atgtagcata
    46801 cttggataat ataattactt acaagccaaa tgtaaataat atatatggtt gtcacaattt
    46861 ttatcctcaa aggggtacag gcttgccata tgattttttt gtaagatgtt caaatagatt
    46921 taaaaagtat ggaataagaa ctgctgcttt tgttaattca aaatacgcaa caatagggcc
    46981 ttggaatata aatgatgggc tgtgtacgct tgaaatgcat agaaacttag atattgctac
    47041 agcaaccaag cacttattcg caacaggatt gattgatgat gtaattgtag ggaatgcata
    47101 tgcaagtgaa gatgaattaa aaagaatgtc tgaagtaaat aaatatcaag ttgtatttaa
    47161 tgtagtaact gaagaaaaaa cttctgaagt agaaaagaca ataatggcaa atgagcaaca
    47221 ctttagaaga ggggatataa ccaaccaagt agcaaggtct acagaagtaa gaaaaaaata
    47281 tgctaaagaa aatttcccta agcatgataa tacgaccatc tttaaaagag gagatgtagt
    47341 gattggtaat gatgactttg gaaagtataa aggggaatta cagttgatac ttcaagattg
    47401 tgcagatgaa agaaaaaata aggtgggtag aataaaggaa gacgaaataa tacttttaga
    47461 ctttattgaa ccttggagta aatttaaact tattttagag ggagagtaaa gtgaagatag
    47521 acatattaat aaaatgtgga aaaacagtag ataaaagtat aattgatata gctatattag
    47581 acgataaaat aatagaagta aaaaatcaga tagatgattc taagtatcag gcaaatcaaa
    47641 ttataaacct agatggagaa aaatttatta gtgctggttg gatagatata catacgcact
    47701 gctacgaaag actagacctt tataaggatt atccagatga ggttggaata aaaagcggtg
    47761 ttactactgt agtagatgca ggaacaacag gagctttaga tataggtaaa ttttatgaag
    47821 atactaaaaa atataaaaca aatgtatatg ctttaataaa tattgctaag cagggaataa
    47881 catctcaaga tgaactttca agtatgatga atattgatga atatgaactt agaagagcag
    47941 ttaaaaaata caaagatttt gttgttggaa tcaaagctag aatgagtaaa agtgttgtaa
    48001 tttcaaatga tgttgaacct ttaaaagtag ctaaaagaat aaaaaatgaa cttaatctac
    48061 ctatgatggt tcactttggg agttctcctc caacgataga ggatatattt gactatatgg
    48121 aaaaaggaga tatattaacc catatatata atggaaagcc aaatggaata cttagaggca
    48181 atgaggttaa aaaagaaatc atagaagcta gagaaagagg tataatcctt gatgtaggtc
    48241 atggaaccga aagttttagt atggatattg caatgaaatc taaggatgca ggaatttttc
    48301 cagacactat aagtacagat atatatatta aaaatagaat aaatggtcca gtgtataatc
    48361 tatctacaac aatggaaaag tttatatata tgggctattc acttgaagat ataatagata
    48421 aagttactaa aaatgcagca gatgcaataa gtttaaaaaa taaaggttta ataaaagaag
    48481 gatatgatgc tgatttaact atatttgatg tggttaatga agaaaaagaa cttcaagatt
    48541 ctttgaataa gtcagtaatc acaagtacgt ctataaagcc aagagcagta gtagtcaatg
    48601 gagaatattt aaatataggt aagtctatag gagagaatga gtagaaggga gaaactattg
    48661 tatgagtagt atatttgaaa aatactctgt aagacgtgtt ataaatgctt caggcaaaat
    48721 gactatacta ggagtatcaa cagtagataa ggaagttatt gaagcaatag gtgaagggtg
    48781 taaaaatttc tttataatag aagaacttat aaataaaaca ggtgaatata tagcaaattt
    48841 attagggtgt gaaagtgcac taatagtttc tagtgcatct gctgggattg cacagagtgt
    48901 gggagcaatg atagcaaaag atgatatgag tctagtttac aatgtaaata atcctaagaa
    48961 acaagttaaa agggaaataa taattccaaa gggacataat gttgattatg gagttccagt
    49021 agaagtaatg ataagacttg gaggaggaga agttgttgag gccggttatg ctaatatgtg
    49081 taacaaggaa catataataa gagagataaa tgaaaataca gcagcaatta tgtatattaa
    49141 atctcatcat tgtgttcaaa agagtatgct aagtgtatta gatgcagtac aagttgctaa
    49201 agaatataat ttacctttga tagtagatgc agcagcagaa gaagatttaa atgtatatta
    49261 tgaattggga gcagacctag tgatatacag tggtgctaag gctatagagg gtcctagctc
    49321 agggttagta ataggtaaga aaaaatacat agataatgta aagcttcaat caaagggtat
    49381 aggaagagct atgaaaatag gaaaagaaaa tattgtaggt ctaacatgtg caattgagag
    49441 atatataagt aatgataaag taactttaaa agaaatggag gataagctaa atccatttat
    49501 agacaagata aatactataa agggagtgag tgctagtata actagggatt cagcaggtag
    49561 agaaatactt cgtggagaaa ttgactttaa tgaagatata ataaataagt caacacaaca
    49621 aattataaat gaacttagaa gtggagagat agctatatac actagagact ataaggctaa
    49681 tgaaggcaag attgagattg atataagaag tgtaactgga agtgatttag atactattta
    49741 taataggata aaaaatatag tagaaagtta gagtgtagag taagtgacat tataatgatt
    49801 taataaatta aattttacca atggagggga taaatatgaa ttgtatacca caatattata
    49861 aaaatagagt ttgtttaaat gttcttgcag gaagcataaa aaatgctaaa gaagtatatg
    49921 atgcttgtga cgggcatgta ttgataggaa tactatcaaa gaattatagc actgtagaag
    49981 cagctattga agatatgaaa aaatactcaa aggaaataga taatgcaata tctgtggggc
    50041 ttggagctgg agacccaaat cagtcaaata tggtatctca gatttctaaa gaacttcagc
    50101 cacaacatat aaatcaagtg tttacaggtg tggctacgtc tagagcattg cttggtcaaa
    50161 atgatagtat aataaatgga ttagtttctc caactggtaa accaggattt gtaaaaatat
    50221 ccacaggtcc attaagttca aaagaaaaag atggtatagt tcctatagaa acagcaatag
    50281 ctatgatgaa agatatggga ggaagctcaa ttaagttttt tccaatggga gggcttaaaa
    50341 caaaggatga atatatagaa gttgcaaaag cttgtgcaaa gcataatttt tatttagaac
    50401 caacaggggg tatagactta gataacttca aagagatagt tcaaattgca cttgatgctg
    50461 gtgtagaaaa agttatacct catgtttata catcaataat tgataaagaa acaggtgaaa
    50521 cgaaagtgga agatatagga gtactttata aaataatgaa agaattattg ggatagattt
    50581 atgaatatat tagcttttgg tgaaataatg atgagacttt cagtacctga ctacaagttc
    50641 ttaacacaaa ccaatgaact aaattatata actacaggca cgggattaaa tatattatct
    50701 ggacttaaaa actttggata caatacctat atgttgacaa agttgcctaa taataatgta
    50761 ggtaaagcat caagtgcaaa tataagaaag cttggagtaa aagatgactt tatcacgtat
    50821 ggagggaatc atataggagt gtattttctt gaaaatgggt atggagaaag accttctgaa
    50881 gtgacttatt tgaataggtt aaatagttca ttttgtgaga gtaaattaca agattatgat
    50941 attgataaat gtttagaaga aatggatata gtccatattt gtggtattgc tatgcaactt
    51001 acagattcag ttaaagagat tgctttagaa ctagctagaa aatcttatga aaaggggata
    51061 aaagtattct ttgattttaa ttttagacaa tcattaaatc cagaaaggac ttatgatgat
    51121 ttagtaaatg attataagaa aatactacca tattgtcatg ggctgtttgc tagttataga
    51181 gatataaata agatacttaa tatagatagt ctaaatataa aatctagttc agaattgatt
    51241 aaaaaagagt atgatataga tattgtagct ggaactatga gaagatttga tgatatgaat
    51301 aatagattta taaagggata tatatcaaat gatgatggtt attttgaaag tagggagtat
    51361 aagctagaaa tatatgacag ggttggtgcc ggagatggat atgtagcagg aattatatat
    51421 tcatatttgg aaaatatgaa gagtgaagat atagttgagt ttggggttgc tagttcggtg
    51481 ttagctcata caacttatgg ggataattct ttagtaagca caggtcatgt acttagactg
    51541 atgaataatg aaaaaataga tatattgaga taatacattt gtatataata aaatttttat
    51601 cttttttaga cacatttttg acttgcttat atttgttaaa cagagttatt tttaatgatt
    51661 ataaatatta aattaaaagt taaataataa aatagtagaa aatagcatta actatagaaa
    51721 aaatggttaa tgctattttt atcgttaata ttgattaatt ttatattata gaaatgaact
    51781 tttctaatgc tggatttgta ttttttttat tccatacaac tacaacttca attctatttt
    51841 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51901 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    51961 tatttgaacg ttgtaccaca ttaggtacaa accctgcttc acgatatcta ttctgtatca
    52021 ttgacttttg tccatactca tcccctgttt cctttacaaa aataaattta tcatgttgta
    52081 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52141 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52201 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52261 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaaatatt
    52321 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52381 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52441 cctcagataa aaaaactttt ccagctggtg ttagttcaac atgacgttta cttcttgtaa
    52501 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52561 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52621 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52681 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52741 aaaatatttg tataaaaata tatgttttaa gtatatcttt acattagatt ttataaattt
    52801 attaataaga ataacttata tattccaatg aaaacgatat tggatttaag taagtttgtc
    52861 tcataaaatg aaagtatatc aattgataat actttaaata agaagggaga aaaaatggaa
    52921 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
    52981 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
    53041 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
    53101 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
    53161 aaagagtatc ctacaacaac aactcccaaa attcaaatag cagatgattt aatgatagta
    53221 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
    53281 aagtgtgaag caggaattag aatatatagt ttaaaagaag accctttaaa tcctaagttt
    53341 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
    53401 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
    53461 gtcatagata taataaatcc tactaatcca gtggaaatag gtaaatggtg gagaccagac
    53521 caatatgcaa atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
    53581 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
    53641 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
    53701 ttaggtgaat tgccatttat gcctgcattt tctagtagac ttgcaggtgc aagaactcat
    53761 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
    53821 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
    53881 gatgttagtg acccaacaaa accaacatta attgctcaat ttccatatcc tgaagttcca
    53941 aaagatttcc cttatcctaa ctttaatgtt gcgggattag gaaaaccagg gccatttggc
    54001 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
    54061 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
    54121 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
    54181 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
    54241 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
    54301 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
    54361 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
    54421 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
    54481 gcgtgtgtta aaactttatt atctgggaaa ggtagtatgt gtgtattaaa agatgatttt
    54541 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
    54601 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
    54661 agaggaaata acattatagg aacaaaagtt gcagaagcta taggaggaaa aattccagat
    54721 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
    54781 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
    54841 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
    54901 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
    54961 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gccaggtact
    55021 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
    55081 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
    55141 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
    55201 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
    55261 caagctggag tttcattagg ataataatta aaatactaag actctttttt aaggtatgtt
    55321 agatgttata ggaggcttat tgtatgaatg aaaagtcaag ttttaaacaa attgaaatta
    55381 ttttagcagt tatttttttg gtacttacat attacaaggt tagattaact ccagcattta
    55441 ttgttatatt tataccacta tatttaagtt attttgcagt atctatgatt gtaaatcaaa
    55501 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
    55561 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
    55621 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
    55681 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
    55741 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
    55801 aatcaaaacc tagaattatt tttaacactt agtatattag gtttttttgc agttgtaatt
    55861 gcatccttat ttgaaaaaat aattttccta ccatcatggc tttgtggttg ggctataggt
    55921 ctaactatta tgacaggtga aaatattatt aatttacaag atatttgtat tcaaattgct
    55981 gtagctatgg cagtaggagt atggtacgtg ggtgcaggtg tagatttgtt tacaatatat
    56041 attttgagta aagataggaa aaagggaaat tgaaagcatg tgaatatact ttgattatag
    56101 gagaatggct atgggttttg aatttaaaat aatgagaagt ttaatatatg taggacttgc
    56161 caaggaagaa tatagaccta agctaatgga ctggttatat cgtcaccata ttccagatag
    56221 tattagcact tttggaccat attgtactaa atatgccttt tatcaagcat atcctacacc
    56281 aaatgaaggt gagcgttttg gtgcacgtaa gatgcaacta acagaacatt attggcttgt
    56341 agatgaacat atgcctgaga tggcaaatag aattatgaca gaatatatgc ctatggatgt
    56401 tctacgttgg caagggtgta taccagatgt agaaaataaa agggttcatg aaaatgcaga
    56461 aagtggagat gcaggacgtg cagtaggtgg agataatgga tgtccaccat ttatatttgc
    56521 ctttgttcca ataaactggg aagaagactt tagaggaaaa ggacgtactg tacaagatgg
    56581 accaaactat cgttggcaat ttatgattaa gtatccagat ggtatctcta aagaagaagg
    56641 agaaaaatgg ttctatgatg aggtagtgcc atactttaca aactgttgct atgttaatcg
    56701 ttttgtcagt agtaaaataa tgattaatta tggagcaact gcttttgacc gtgtatcaga
    56761 actatggttt gaaggggaag aagaatggta taaagctgtg gttgaagaga caaagtcgtt
    56821 tattaaaaaa ccagaatggg cacaagaaga ggagttccca tatttaaaac cacaattcaa
    56881 tatcgcatca gtattcttag gtgatatagc aactatggat gcatactcac agtatcgtgg
    56941 atatatacca atgagataaa aattatagtt tgtgataagt agaggaggca tttactctta
    57001 ctggtagaag aagacttatt gtatccttaa aagtaggtga atatatagtt ttatatttaa
    57061 atttatataa aactaagact aataaatatg aaacaataga attagaataa tttgataata
    57121 tttgttaaaa gagcatttag aatgtgttta aagattaaga tattcctttt atggtagcag
    57181 atagttgaaa taataaaact gttatcatga aaggaatatt tttatgtata ggaaattttt
    57241 aaaaataaaa gttaaatatg tttaagaaca tcttaaagtg aaagaatcat tgttagaaat
    57301 atgtagacaa tatagtcttt ataacgtatc atattaacaa gtttacactt ggattatgaa
    57361 ataaaaagag taattccaaa ggttgtaaat actatgctga ctttgatact tcaaagttta
    57421 ggtgattttg taattgttaa tagaaatacg tttaatgagc ataaagaatt tatctaataa
    57481 taggagaata taatgtatac aattggacaa gtatctaaat ttttaggaat atctagagat
    57541 acactaaaat tttatgagga caaaggatta gtaaatccta agaaaaatga tgaaaatgga
    57601 tatagaatat ataatcaggt tgatatctat gatattgcaa caataaactt ttatagagaa
    57661 attgatatag aaattaaaaa gattcaagaa ataagaaaaa gtaagagtat aaacaattta
    57721 gaactcttac tagaagagaa ggaacaaatc atactaaaag agatagaata taaaaagctt
    57781 ctcttaaaaa aggttcagtt agtcaaagaa gattgtgaca aaataaaaac atattttgga
    57841 aaatacacaa taaaagaaat gaagcctttg gaagtaaaag gtaaaataaa acatgttact
    57901 aactatgagg aatatgaagc cttaaaagag aatacaaaca acttaaaaaa agcagttaca
    57961 ttaactgatt taagaagagt aataaatttt aatgaagaag gtataataga agaaaaattt
    58021 ataattgtaa gggaagtaga tgactacaac aaaatcatag aaggtgaagt tctatctcat
    58081 cccaattgta tttatactat tattgaagat ggcagatggt caactggagg agaaaatatt
    58141 gataataaag tggaagacag attaagagaa atagcaatag aaaatgaatg tgaattactt
    58201 ggaatagtct atgttaatat attgcttact acttatgaag aagggattga gcgtatattt
    58261 ttagagattt atgcacctat aaaataatat aaaagcattg acctgggact tactcatagg
    58321 tttaaactca tgatataagc agttataact tatatagcaa ttaaatatta atttgaaatg
    58381 aggattactt atgagaacag agaaatcatt aaaaaggcct atagccagtt ttattattct
    58441 aacaaatata atttttttac cacttttttt attggtagta gtgactataa tgttaaaact
    58501 tccaacagtg atatctgaca ttgcgttgtg tatatcagcg tggtcatcca cttttgcatt
    58561 tatgatatta tttaaaaaaa tttatccagg taagaaattt ttagtacacg taaaagatag
    58621 atttagaaat aaacttaaat tttcaacagt atctataata attagtatac aagtcctaat
    58681 agctgtagta gttatattta tattagcatc taaaaatcat ggaataatgc ctaactttac
    58741 agtttcatca tggggagcat ttatatattt atttttaaaa aatttatttg caggatcatt
    58801 aggtgaggaa ttaggttgga gaggatttgt tcaaaatcat cttcagaaaa gacattctcc
    58861 tttaaaagca tccataatag taggtttttg gtgggggatg tggcatttac caatatggtt
    58921 tacaacagga ttcaaaggac ttgatttaat aaaatatatt atatttttta tgattgcaat
    58981 tatatcaatc agtattataa tgacaacttt ttataatcta aataaaactt tgataatacc
    59041 tattgtaata catcaactat ttaatttttt aatagggata attaatggag atttaataga
    59101 gcttataaat tactatgcaa tgttatattt aatatttgca attgcattaa tagttataaa
    59161 tccaggaaat gtattatata aaaatattag agagtaatat atatctttat ggtaagatat
    59221 aaagatatta ttagaagaac atccaaggag gaataaacat ggttttactg attgttgatg
    59281 cacaaaagtt aataacaaac gagaggttat ataaatttaa tgaatttgta gtaaatgtag
    59341 aaaatttaat tgatacagct agaaaaaata atatagaagt aatatatgta cgtcatgatg
    59401 atggagttga aaatgaattg actaaaggta aaaatggctt tgaaatatac gaaaaattta
    59461 aaccatgtaa tggagaaaaa atatttgata aaaaagtaaa tagtgctttt aaagaaactg
    59521 gtctattaga atacctaaca aataatggtg aaaaagatat tattattgct ggtctccaaa
    59581 cagattattg tattgatgcc actataaaat gtggatttga acatggattt aatataatag
    59641 ttccagctta ttcaaataca actgttgaca atttttttat gtcagcagaa caatcatata
    59701 aatactacaa tgaatttata tggaatgaaa gatatgcgaa gtgtatttca ttagaggaaa
    59761 cacttagaaa aatgaaataa aaatagagta tgaattgtat atgtacataa gttgatttca
    59821 taaaaaagga tgttaaattc caagttaaaa attagattaa atatattata ttatagataa
    59881 ggcacatcaa ttttatttta aattaatgtg ctttttaatt gtagtaattt atttataatc
    59941 aatatgattt ttattattta aatcaatttt tcttgagttt ctttgtctaa agaaactttt
    60001 tttatttata aaatctacag ttatattata gtatctattt tagagaagtt taatttttgt
    60061 atcaatctaa aacaaaagtt atacttaaat gaaattgaaa acgataaaat tctcaaataa
    60121 aataagtatt gtggaggtga tgaaagaatc caatgataat atgaagacta atcttaataa
    60181 gaaagtaggg gttaatggta gtggacaaag aaattgtatc aattctccag attttattaa
    60241 atagcacatt gattacaact aatggattac aagaggagtc taattcatca aagagacaaa
    60301 ttacttatag aataaataaa atcaatgaca tgcttaaagt aaaaaatgta ccacttatat
    60361 gtttaagagc tgataaagat attatcgtta gaaaagaaac aaaaaaagca attaaagaaa
    60421 ttttagaaaa aaactattct aagaatactt attattttag taagacagaa agacttttat
    60481 atatgtattt aatgcttttt attaacatgg atgatatttc aattaatcat tttataaatt
    60541 ctataaaagt aagtaggtct actgtaaatc ttgatttcaa ggatttgata ccagagcttg
    60601 aaaagaagaa cattaaagtt aagaacaatc gtataaatgg atattacata gttggcaatg
    60661 agatggaaat taggagagtg cttattaaaa atattataga aactttgtct aatgagaaaa
    60721 gttcaagagt atttgatatt tttattaaag aatataattt agatagtttt gaagaggcta
    60781 aaaaaattat attaaagcta ataaaaaaat atgaaattac ttttgttgaa gatagattag
    60841 tagaatttat atatatattt atctttctaa aggcaaggat gtattcaact agaatagtta
    60901 ctcaggaaaa tgtagatatt cctaatattt cagttatgag ttcaatgaag gagtataaat
    60961 ttgctaagga attattagaa gtatatggga gtgaagataa gataaagcct cacaatataa
    61021 tgtatattag tgcttggatt ttaggtatat cagtaggaaa ttatgaagaa gatacagaag
    61081 ataaggatgt aatttcaaaa attgtaaata aaatgatgac aaagtttgga tatttaagcg
    61141 gagtttacta tataagccaa gaaaaaatat ttaaacaatt atattctcat tttagacctg
    61201 cctactatag acttttattt aaattaccta tttataatcc gatttgtgaa aaagtaaaag
    61261 aagaatataa attagtttat agaattgtaa gtaatgcaat gaaagagttt tgcggattat
    61321 ttggagaaga aattcaggaa gaagagttag catatcttac aatgcacttt gcaacacttt
    61381 tttcaaataa aaaagaattt gatggtttta gaaaaagaat agcattaata gtttgttcaa
    61441 atggcgttgg tagttcagct attttacatg cagaacttac aaacttattt cctgacttac
    61501 actttatacc aatgtcggaa tcagttggat tagaaaatat aagtgaacct gtagatattg
    61561 tatttactac taattataac gcagagggtt taaaaacaga tgtaccagta attaaagtaa
    61621 gccctgttat gaccacaaaa gaaaaatata agattacacg tgaagtgtat attcaattag
    61681 gtgatatttt tttaagacaa cctaaaatag atgaggttat ggatataata aaaaaatatg
    61741 caaatgtaac ctctgaaagt attctttcaa gtgagctatt agcttatttt acacagatag
    61801 agaattttac atctaaggaa ggagaggggc ctatgctaag tgagattact aatgaaacac
    61861 tagtaaaaat aaaggtaaaa gctaagaatt gggaggaagc tattagacag tcagcttctg
    61921 tattagttga aaataacaaa gtaactgaag ggtatgtaga tgctatgatt aactcagcaa
    61981 aagcatctgg accatacata gtaattacaa aacatgttgc acttccacat gctagaccag
    62041 aagctgggtc aaaagagatt gcaataggca ttgctacttt agatagtcct attgagtttg
    62101 ggaataacga aaacgaccct gtaaaatatg tattttgtct aagtgcaata gacaataata
    62161 gccatttacg tgctatgtct gagctagttg aactattgga agaagatgag ttttttaagg
    62221 ttttagatag tgctaaagaa gctaaagaaa ttattgattt tataaaaaca cacgaattat
    62281 aaaggaggat ttaattatgt ataaaatttt aatcgcatgt agagcaggag ttggctcaag
    62341 tttaatgtta aaaataaaga ctcaacaaat tattaaggaa aataactttc caattgaagt
    62401 agaacatggt tcattggatt cactaaatgg attcaatgga gatgctgtag aaacattaat
    62461 tgatgttgct gaagaattaa aaaataaaaa tttgaagttt gatataattg ggattcacaa
    62521 tattgttgac cgtaatgaaa taaagacagc actagaaaaa ttccttgctt caaaagaggg
    62581 ataaatttaa gatgataagg aggaatattt atggagttta ttattagttt gctgagtaca
    62641 cctgctgttt tgcttggcgt agttgcatgt ataggattga ttcttcaaaa gaaatcagct
    62701 gttgaagtat ttacaggctc ttcaaaaaca cttatcggtt ttttgatatt tggtattggt
    62761 gcctctgcta tgacaggagc acttcaaaat ttcaataaac tgttccaaca tggctttgga
    62821 attactggtg ttatagcttc accagaagca gctacagcac ttgcacaagg tacatatggt
    62881 tttgcagttt catgtacatt aattcttgga ttcatattaa atttagtatt tgcacgtatt
    62941 acaaagttta agaatatttt ctttacaaca ggtcatagtt tgttcttttc ttgtgtatta
    63001 gttttaatta tgaaagcaca tggatttgat aatacaatca ctattttaat aggtggaact
    63061 atcttagggt ttatgtctgc tgcactacct caattctgtc aaccatttat gcgtgaatta
    63121 acaggtggcg acgaacaagc tattggtcac tttaacatga ttggttatgg tttatcaggt
    63181 tatattgggc gtttattttc aaaacataaa gatgatacaa cagagactat agaatttcct
    63241 aagtggttat caatattcag agacttctta atgggtctat ccatagttat gttaatctta
    63301 ttttatattg caacattaaa agctggtcaa gcatttactc aagaaattgc tggttcaaca
    63361 cattggttgg tttttccgct tatagaggca tttacatttg tggcaggtat gtcaatattg
    63421 atgtcaggag ttcgtatgtt cttagctgaa ataactgcag catttgtaat catttcagaa
    63481 aagtatattc caggttcacg tcctgcgcta gatgttccaa ccgtatttcc atatgcacca
    63541 aatgcagtta ttattgggtt tatatcagca tatgctgctg gtctattagc aattgctatt
    63601 atggcatcat ttcctagtat atttcctgta gtaattattc cagctgctca catatgtttc
    63661 ttctcaggag gtacagctgc aatatttggc aatacttcag gaggatggag aggggctatt
    63721 gctgggtcat ttgttgtggg gttattactt gcatttttac cagttatact atatccagtt
    63781 tatgggactc ttggtataga aggagcaact ttccctaata ttgattacaa cgtagttggt
    63841 tcgatattac ataatatttt acaatttata aaacctattt agactaaatt atataaaagt
    63901 atagtcatag ttactatgat ttcaaggagg atacatctta tggaaaatac attatctgaa
    63961 tttttagata atatatctaa tgaattgtca gaatttattg cctcaataga tgagacatct
    64021 ataaataaag catgcgaatt aattcttgaa gctgaaaaaa atcacggacg tgtacatgtg
    64081 actggtattg gcaaaccagg acatgtatcg ggatatattt catcattgct ttcatctact
    64141 ggaacgtcag catatatatt gcatggtaca gaagcagtac atgggtcaag tggtcaagta
    64201 gttgaaggag atgttgttat tgcaatatca aatagtggtg aaactcaaga gttaaaagca
    64261 actcttaaga ctctaaaagt aaatggagca aagataattg gtgtaagtgg taatgagagt
    64321 tcatttttaa aaaatatttc tgatatattc ctgtttgcag gtgttaagca ggaaggagat
    64381 tgtctcaata aagctcctcg tgcttcaatt ttagctgaaa caataatatt acaatcatta
    64441 agtgttgtat tacaatatgc taaaggtctt aatacacagg agtatttgaa gtggcatcca
    64501 gcaggaagtc ttggaaagtc tattagagag ggaatataat tttatgaaat ataaagggat
    64561 tatatgtgca atgataacac catttgatga aaatcaaaat attaattcac aagcaacatg
    64621 tcaattaatt gattatttga ttgaaaaggg aatttatgga ttatttattt tgggcactaa
    64681 tggtgaatgt catgtattaa cagatgatga aaaagttgaa tttgcaaaga ttgttattaa
    64741 tcatacaaat aatcgtgtac ctgtttttgt tggaacaggg gggaattcaa ctcgtgaagt
    64801 tgtagattta tctaagagaa tggaagcgat tggagcaagt gcattatcta ttattacacc
    64861 atactttgtt acaccaacac aacaggaatt aatcttacat tacaaaacta ttgctaattc
    64921 agttaattta cctattataa tgtataatat gccaggtaag actggaatta atattgaacc
    64981 agagtcagta cgtgaattat caaagataaa gaatattgtt ggaattaagg atagtagtgg
    65041 taaacttgac aacatgaaag cttatattga agcaacgaag aatgagaatt ttagtgtgtt
    65101 ttcaggctca gattcactta ttctggatag tctaaaagct ggtagccaag gagctgttgc
    65161 agcaacaaca aacttcctca cagagattgg tatctctatc tatgaaaact ttatgaaagg
    65221 ggatttggaa gaagctcaaa aatctcaaag tagtatagaa gaacttcgac gtatactgaa
    65281 acttggaaca attccatctg ttatgaagaa aactattgtc ttgaatggaa ttaatgttgg
    65341 aactgcacga cttcctgtaa ctgaaccaac tggggaagta cttgaagaaa ttaaacgagt
    65401 agttgaaaat tataggacaa tacttaataa aagtagtgag aatagataat ataaataggg
    65461 aaattacact ggtatataat taatatatat attataaaac atgactaaaa ttaatgaata
    65521 agtaaagtgg tttgatgagt atagaggtga taatatgaaa aagatgaata taaccaaaag
    65581 aatgtcagaa tgtggggctc ttgcaattgt aagagaagaa aatcttaacc gtgcatgtga
    65641 aattgctgaa ggatgtataa aaggtggtat aacagttata gaaatgagtt ataccctaaa
    65701 taatgctgga gaaataattc aagggcttaa taaaaagtat ggtgaaatac tttgtgtggg
    65761 agcaggaaca gttttggata gtgaaacagc tcgtcatgct atattacatg gagcccaatt
    65821 tattattgca ccaaattata atgaaggtgt agcgaagatt tgcaatagat atcagattcc
    65881 ttatgctcca ggatgtacat cattgactga ggcagtagac gcactaagtt taggagcaac
    65941 ttttataaaa gctttcccaa tatcagattt ctatggacct aaactagtaa aggtatttaa
    66001 gacaccaata cctgatatgc cagttttagc aagtggaggg cttaatattg aaaacctaca
    66061 tatatggtta gaaaatggag ttgatgtatg tggatttggt ggtttattaa ctaaaggttc
    66121 tgttgaagat attgctaaaa atgctaacaa gattcgtgaa atcattaaaa atgtaagaaa
    66181 aatttaacat agggctgtat catagtatct tataattact atgatacagc ctttttaata
    66241 ttattcaaat ttaaaacagt ttacatttct attatattac atataatttc agtaatttta
    66301 tcttaatttt tctatctgga aaaaaatgat ttaaaactta agttatgata taatttcatt
    66361 aaaagaaggc gatttttagt aaatatgaga aaataatgtg tttttagggg aaaagttaaa
    66421 tacagattta agtgaacaag aatcgataat taagatatta aatggtgaat agggcatcaa
    66481 aaaaattact aaattaaaag ttttagaggg agaatcttag caagtgtgtg tgaaaattca
    66541 atttcaaaca aaaataattg taaatgaaat caatttatac acgttgaaga ttcactttta
    66601 tcattatatt aaaaagcaga gtgaattaaa agtgagtcta aagataggag gataattaaa
    66661 acaatgaaag aagacattag gttacatcga atgtgtaaaa aagaacaagc aacaaagaat
    66721 gaagttatat cagaggatat tctaaaatct atgagtattg gggtcagtaa acatttttta
    66781 gatgattcat ttacaataat atggggaaat acatcattct atcaattatt aggatataca
    66841 gaagaagaat ttttagttaa attttctagt ttaaaagatt attatgaaca gaatctttat
    66901 cattttgaat ctatgaaaaa tcatttttta gaggcttatc atcatggcga aaagagtata
    66961 gaatatcaaa tttgcatttc agcaaataat ggagaccctg tttgggtaat aatgactggt
    67021 atattaattg aatcacaaga taatgagaaa tcaatggtct atatattgta ttcaaaaatt
    67081 aatgagttaa aaataaagca agaagaacta tctgatatgg aaaaagagag tacagagaat
    67141 tttaaatgga tgatgtcaga gtatgctggg aatgtttata ttagcgatat ggatacatat
    67201 gaattactat acttaaataa gcactcatgc aatactttac aggcatcagc aaatgaactt
    67261 attggaagaa aatgttatga ggtaattcaa ggtagaactt cgccatgtcc gttttgtaca
    67321 aattcgtatt taaaaaaaga tgacatatat gaatgggaat tttataataa aaaactgaaa
    67381 cgaacattta taattaaaga ccgtatgttg aattggcaag ggcgaagagt tcgtattgaa
    67441 ttatcttatg atatgtatag tgctgaatat aaattagcaa aaaaagatca ggaaagagaa
    67501 tcaattttga aaactattcc agcaggaatg gttagattag atgcaagaga ttatcttact
    67561 atactttggt gtaatgatat atttttgaat atgattgaat atacaaagga acagtttgca
    67621 gaggaactta ataatcaatg tggatattta ttttctgaag attataaacg tagtaagaaa
    67681 ctggcacaag atttgaagga gtctggggat aatgttgtat ttgaagcaaa aatatataca
    67741 cgttcaaggg aagaacgtat atggactgtt acattgtgtt atatcagtgg ggaagacagt
    67801 tgggatggaa taccatcttt ttatagtata ggtattgaca taacaaagga aagaaaacag
    67861 attgagaaat tacagcatat atcagagaaa gatgcattaa caggaattta taatcgtgct
    67921 gagactgaaa ggcagattaa gaaatatttt gaaaaaaatt taaatgttat gggtgcactg
    67981 tttatgattg atacagataa ttttaaacag attaatgata cagaagggca tatgattgga
    68041 gatatagtac ttactgagat ggcatcaggt atgaaaaaaa taatgcgtga cagtgatgtt
    68101 gttggaagaa taggtggaga tgagtttaca atttttatga agaatatttc ctcagttaaa
    68161 gatgcagaaa agaaagcaga agaactattg tatatgttcc gtcatctatt tcagaaagaa
    68221 aaaagttttc taaaagtaac ttgtagcata ggaatagcta tatatccaaa ggatggtact
    68281 acattcaagg aaatatatgc aagagctgat aaagccctgt atcaagcaaa aaatatggga
    68341 aaaaataatt atgtaatcta caaccaagat tattttaaag aattagaaga atttgattgt
    68401 tcttctcttg gtacagttat tgattcagaa aaacaatacg ctgaatatcc tgataatctt
    68461 acacgttata tattccgtat gctttatcag actgatgata tagaccaggc gattaatatg
    68521 atacttgagg ttgttgggaa acagtttgat gtaagtcgtg tttatatttt tgaaaataca
    68581 gaagatggta gatatacttc taatacttat gaatggtgta atgaaggaat ctcttctcaa
    68641 atggcttatt tgcaaaacag caattatcaa gactatgagg attatgaaaa gctttttgag
    68701 gatgaattag ttttttattg tcgagatatc catacattaa gtcctaaact agaagaattg
    68761 ttttctagtc aaggtatcca ctctacactt caatgtgcat ataaagaaga tagagttttc
    68821 agtggatttg ttggatttga tgaatgcaca ggactacgtc tatggtcaca agaggaaata
    68881 agtacattat cattgatttc tcaaattata tcaatgtttt tacaacgtaa aaaaaataat
    68941 aaacttaatc aacagattca acaataccag aatattttag ataatattga ccagtgtatt
    69001 tgtgtgatta ataaagaaaa taatttttta ttatatgtaa ataaaaaatt taaaaataca
    69061 tattctaaat tccaaattgg acaaccttat tatcttgatt ctatagatat tcataagata
    69121 tccattatat ggaataaaaa agaagcatac ctatgtacta gcacgattta atggtaaagt
    69181 aaagtttata ttggacttaa attaaatttc gttttaaata agatattatt ttagttgctt
    69241 actgaaaaga ctagaatcta tattaatgaa ttatactaat tttataccac tattaaaata
    69301 ttgtttgcta aaaataaaaa atagccttaa aacagttgat tataaaaaat taactgcttt
    69361 aaggctattt ttatctttgt gacgaaatag acacttgcca tttagaatgt gacaatatag
    69421 tagttgttta gaaaaggcac aaagcattat actttaacta aagtaaaatg tattttgctt
    69481 ttagaataaa aacaattagg agggagctat aattgaaaaa gataatatat gtattattta
    69541 caattgaact attaatagta tctttattag gtctaaatat agttaaagat aatgaaatta
    69601 ataatatttt atataacgat acaacaagta tatctgtaat gtttaaagat tataaaaagt
    69661 taaataaaaa ttatagtaag tggattaaag atattgcaga tgaaaataat gtggctatat
    69721 caaagtatgt ttttaataat aatgaaaaat tatctatata tacgactgat acaagtttga
    69781 ataacaatat aaagttaaaa tcaggaaaat ttcctaatac taattcaaat gaatttatta
    69841 gtaatataaa cactaataat agtaatcaag taggtaaatt ctcaactatg agtaaagaca
    69901 tatcaatttt aattagggat tttggtaaaa taagtgaagt tggagaatca ggaatcttat
    69961 atatttcaac tcaaaatgaa gatacaataa ataaaatatt aagagaacta aatgtagaca
    70021 gaagtatttt tgtagcaaga ttacatgata gatatgttaa ctcaaattta tacttaaatc
    70081 cttcaataat tagagattta atactaatta tcctttgttt cttggctaca ataatacatt
    70141 attcaattag tgtgagtaga gaggcttcta ttttaagatt aaatggatac tctaagctag
    70201 atataattgc tagagttata aaaaatatac ttagaattat gattttatca tctgtcacag
    70261 cattattaat atatataatc tatattttaa aattaaatat aggtgttaga gcatgcttat
    70321 actttgctat gttttcgatt atatgtattt tgataaatat tttgatatct atattaatag
    70381 ttggttttaa tagtagaagt tctaaatatg tgctatctct aaatggaaaa aaatcttatg
    70441 gttttgtaaa tataatgcat ttcacattga aaattgtatt tgtatcattt cttatattat
    70501 ccataaataa ctgtatttta aattataata tgctacaaac tcagttagga aatctatctg
    70561 agtgggataa agcaaaaaat gtttataatc ttactcttaa agatacaggc gaacaatctt
    70621 taggaaagga agaagtaatt aaaaatgcca agattaaagg tttctataaa aatttggtag
    70681 aagaaaaaga tgctttctta atagattcaa ctaattttga aaaacttgaa gatggaagtt
    70741 acatgtatga aataaattca aagggtaaaa acccagaagt aagtcaatat ggtaaaaata
    70801 taaaaataaa taaaaactat cttaaagtta accctattta ctcaaatggc aaagaaatat
    70861 ttaatctaat cgattatgac gataagacta taaatttatt agttcctaaa aagttacaag
    70921 tacatgaaaa tgagattaaa aaagagttta gagaattatt ttattttgaa aaaatagaag
    70981 tagaaaatat gtacaataaa gagttaaata gagagttaaa taaaacaaag aagtccgatt
    71041 taaatgttaa tataatatat gtagataata atcaaagcta ttttacttat aactcccttg
    71101 ttatggatga caataggaat ctcattgatg acccaatagc aatagttgac atagataata
    71161 ttgatgattc tttttatctt tcttatatat ctagatgtgt atattttaat tctaagaaat
    71221 tagatgccct tgcagatata agtagcatta ttgaagctca aggtgtagaa gcgcatatcc
    71281 aaagtttaca tgctgtttat aatgagtatg gtttagaaat aaataaacta gaaaaatatt
    71341 tgaatagtga aatttttact ataataataa tagcaatatc aaacttaatg attacctaca
    71401 atatagtagc tagctactat gaaagaaata aatataagtt atatattaaa aaaatatttg
    71461 gttactcaac tacagctaga agtatgttgt taatagtctc attaatatta acaaacatta
    71521 ttccaatagg cattatatca acaagagtag atttaagtaa taatattatc ttatttggac
    71581 ttctaatatt agttatagaa gtaattgtat ctattgtatt agacaagatt atctcaaata
    71641 gttcatttaa taaaattata aaaggagaac attaaaaaga tgatttcatt aataaatata
    71701 tctaaaaaat ttaaagataa atctataata gacaaattta acctagaaat ttctaagggc
    71761 gagtttgtag cgataacagg aaatagtggt tcaggtaaga caactttact taatataatg
    71821 ggattattag aaaaacctaa ttctggtgat attataataa ataatataaa aaatccaaat
    71881 agtaaacaga taagaatttt acaaagggat ttttatggct atctatttca aaattatgct
    71941 ttaattgaga atgaaaatgt tgaaaataac ttgaaaattg ctttaaagta tcaaaaaaat
    72001 atcaatcata atgaaaaaat aaattacgca cttgagtctg taggtcttag aaaatacaat
    72061 aaaaaaaaga tatacgaatt gagtggagga gaacaacaaa gggtagcatt agcaagagta
    72121 atattaaaag aatgtgaagt aatatttgca gatgaaccta caggtaattt agataaaaaa
    72181 aatagagaca tagtatttga tatacttaaa agtctaaata agaatggtaa aactatcgta
    72241 tttgtaactc atgatttaga tttatcggag caggcagata gagttataaa attgtaataa
    72301 tataaactat aataaatata agaaagagat tacagctaaa ttaagtttaa taataatacg
    72361 aatagaattt aaacattaag taaattagta gtttgtaaat ataagtataa tatgatatca
    72421 taaaggctca aaagaaattt tatataataa gaaatctaaa aataacaaat ggaggacata
    72481 taaagatgga aaatttacca aattgcccga aatgtaattc agaatatact tatgaagatg
    72541 gaactctttt agtttgtcca gaatgctctc atgagtgggc attaggattg ggagctgaag
    72601 aagattcaaa tattatcaaa gatgtaaatg gaaatgtatt aaatgatgga gattctgtta
    72661 cagtaattag agaccttaaa gtaaaaggaa gttcatcatc tataaagatt ggaacaaaag
    72721 tgaaaaatat ccgtttaatc catgactcat cagatggaca tgatatagaa tgtaagatag
    72781 atggttttgg agctatgaag ctaaagtctt ctgttgttaa aaaagcgtaa tattataaat
    72841 aagctgtagt gtttattgtt tttcagtaca gtataaaacc ataagaatct attaagtaat
    72901 aaaagtttgt ttatttatct ttatattttc ttatggtttt attaaatata aattttattg
    72961 aacaccaaaa tattctctcc tctacaaata agccaatctt atttaaacta atatactttg
    73021 tactatttat actgaaattg taaat
//
LOCUS       DAMJYX010000009        72226 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJYX010000009 DAMJYX010000000
VERSION     DAMJYX010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 72226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.009"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(217..858)
                     /locus_tag="PC479_000648"
     CDS             complement(217..858)
                     /locus_tag="PC479_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2803232.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1101..1904)
                     /locus_tag="PC479_000649"
     CDS             complement(1101..1904)
                     /locus_tag="PC479_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2803233.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2060..3235)
                     /gene="thiI"
                     /locus_tag="PC479_000650"
     CDS             complement(2060..3235)
                     /gene="thiI"
                     /locus_tag="PC479_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2803234.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3281..4432)
                     /locus_tag="PC479_000651"
     CDS             complement(3281..4432)
                     /locus_tag="PC479_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2803235.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            complement(5079..5801)
                     /locus_tag="PC479_000652"
     CDS             complement(5079..5801)
                     /locus_tag="PC479_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2803236.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(5860..6537)
                     /locus_tag="PC479_000653"
     CDS             complement(5860..6537)
                     /locus_tag="PC479_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2803237.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(6565..7380)
                     /locus_tag="PC479_000654"
     CDS             complement(6565..7380)
                     /locus_tag="PC479_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2803238.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(7606..7816)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8534..10600)
                     /locus_tag="PC479_000655"
     CDS             complement(8534..10600)
                     /locus_tag="PC479_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2803239.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(10677..13706)
                     /locus_tag="PC479_000656"
     CDS             complement(10677..13706)
                     /locus_tag="PC479_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2803240.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(13759..15219)
                     /locus_tag="PC479_000657"
     CDS             complement(13759..15219)
                     /locus_tag="PC479_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2803241.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(15311..15685)
                     /locus_tag="PC479_000658"
     CDS             complement(15311..15685)
                     /locus_tag="PC479_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803242.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(15861..16601)
                     /locus_tag="PC479_000659"
     CDS             complement(15861..16601)
                     /locus_tag="PC479_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2803243.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(16622..17410)
                     /locus_tag="PC479_000660"
     CDS             complement(16622..17410)
                     /locus_tag="PC479_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2803244.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(17498..17588)
                     /locus_tag="PC479_000661"
     tRNA            complement(17498..17588)
                     /locus_tag="PC479_000661"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17552..17554),aa:Ser,seq:gct)
     gene            17847..18611
                     /locus_tag="PC479_000662"
     CDS             17847..18611
                     /locus_tag="PC479_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2803245.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(18892..20007)
                     /locus_tag="PC479_000663"
     CDS             complement(18892..20007)
                     /locus_tag="PC479_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2803246.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(20048..21574)
                     /gene="glpK"
                     /locus_tag="PC479_000664"
     CDS             complement(20048..21574)
                     /gene="glpK"
                     /locus_tag="PC479_000664"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2803247.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            21867..22964
                     /locus_tag="PC479_000665"
     CDS             21867..22964
                     /locus_tag="PC479_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2803248.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(23455..23967)
                     /locus_tag="PC479_000666"
     CDS             complement(23455..23967)
                     /locus_tag="PC479_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803249.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            24520..25401
                     /locus_tag="PC479_000667"
     CDS             24520..25401
                     /locus_tag="PC479_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2803250.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(25714..26493)
                     /gene="hisJ"
                     /locus_tag="PC479_000668"
     CDS             complement(25714..26493)
                     /gene="hisJ"
                     /locus_tag="PC479_000668"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2803251.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(27039..27389)
                     /locus_tag="PC479_000669"
     CDS             complement(27039..27389)
                     /locus_tag="PC479_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2803252.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(27405..27821)
                     /locus_tag="PC479_000670"
     CDS             complement(27405..27821)
                     /locus_tag="PC479_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2803253.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(27840..28031)
                     /locus_tag="PC479_000671"
     CDS             complement(27840..28031)
                     /locus_tag="PC479_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2803254.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(28182..28997)
                     /locus_tag="PC479_000672"
     CDS             complement(28182..28997)
                     /locus_tag="PC479_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2803255.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(29044..29982)
                     /locus_tag="PC479_000673"
     CDS             complement(29044..29982)
                     /locus_tag="PC479_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2803256.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(29979..30851)
                     /locus_tag="PC479_000674"
     CDS             complement(29979..30851)
                     /locus_tag="PC479_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2803257.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(30876..32804)
                     /locus_tag="PC479_000675"
     CDS             complement(30876..32804)
                     /locus_tag="PC479_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2803258.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(32916..33293)
                     /gene="gcvH"
                     /locus_tag="PC479_000676"
     CDS             complement(32916..33293)
                     /gene="gcvH"
                     /locus_tag="PC479_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2803259.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(33380..35506)
                     /gene="cdhC"
                     /locus_tag="PC479_000677"
     CDS             complement(33380..35506)
                     /gene="cdhC"
                     /locus_tag="PC479_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2803260.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(35563..36369)
                     /locus_tag="PC479_000678"
     CDS             complement(35563..36369)
                     /locus_tag="PC479_000678"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2803261.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(36419..37786)
                     /locus_tag="PC479_000679"
     CDS             complement(36419..37786)
                     /locus_tag="PC479_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2803262.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(37817..38761)
                     /locus_tag="PC479_000680"
     CDS             complement(37817..38761)
                     /locus_tag="PC479_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2803263.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(38781..39551)
                     /locus_tag="PC479_000681"
     CDS             complement(38781..39551)
                     /locus_tag="PC479_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2803264.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(39607..40992)
                     /gene="lpdA"
                     /locus_tag="PC479_000682"
     CDS             complement(39607..40992)
                     /gene="lpdA"
                     /locus_tag="PC479_000682"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2803265.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(41036..41917)
                     /locus_tag="PC479_000683"
     CDS             complement(41036..41917)
                     /locus_tag="PC479_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2803266.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(41952..42626)
                     /locus_tag="PC479_000684"
     CDS             complement(41952..42626)
                     /locus_tag="PC479_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2803267.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(42663..43535)
                     /locus_tag="PC479_000685"
     CDS             complement(42663..43535)
                     /locus_tag="PC479_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2803268.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(43627..44259)
                     /locus_tag="PC479_000686"
     CDS             complement(43627..44259)
                     /locus_tag="PC479_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2803269.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(44364..46040)
                     /locus_tag="PC479_000687"
     CDS             complement(44364..46040)
                     /locus_tag="PC479_000687"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2803270.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(46289..47068)
                     /locus_tag="PC479_000688"
     CDS             complement(46289..47068)
                     /locus_tag="PC479_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2803271.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(47378..49297)
                     /gene="cooS"
                     /locus_tag="PC479_000689"
     CDS             complement(47378..49297)
                     /gene="cooS"
                     /locus_tag="PC479_000689"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2803272.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(49904..50815)
                     /locus_tag="PC479_000690"
     CDS             complement(49904..50815)
                     /locus_tag="PC479_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2803273.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(50931..51509)
                     /locus_tag="PC479_000691"
     CDS             complement(50931..51509)
                     /locus_tag="PC479_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2803274.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            51735..52658
                     /locus_tag="PC479_000692"
     CDS             51735..52658
                     /locus_tag="PC479_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2803275.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(52830..53972)
                     /locus_tag="PC479_000693"
     CDS             complement(52830..53972)
                     /locus_tag="PC479_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2803276.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(53990..55744)
                     /locus_tag="PC479_000694"
     CDS             complement(53990..55744)
                     /locus_tag="PC479_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2803277.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            55880..56086
                     /locus_tag="PC479_000695"
     CDS             55880..56086
                     /locus_tag="PC479_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803278.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(56091..57014)
                     /locus_tag="PC479_000696"
     CDS             complement(56091..57014)
                     /locus_tag="PC479_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803279.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(57268..58968)
                     /locus_tag="PC479_000697"
     CDS             complement(57268..58968)
                     /locus_tag="PC479_000697"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2803280.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(59430..59843)
                     /locus_tag="PC479_000698"
     CDS             complement(59430..59843)
                     /locus_tag="PC479_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2803281.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(59911..62289)
                     /locus_tag="PC479_000699"
     CDS             complement(59911..62289)
                     /locus_tag="PC479_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2803282.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            62608..64059
                     /locus_tag="PC479_000700"
     CDS             62608..64059
                     /locus_tag="PC479_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2803283.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(64263..66776)
                     /locus_tag="PC479_000701"
     CDS             complement(64263..66776)
                     /locus_tag="PC479_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2803284.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(66999..67820)
                     /locus_tag="PC479_000702"
     CDS             complement(66999..67820)
                     /locus_tag="PC479_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2803285.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(68338..68814)
                     /locus_tag="PC479_000703"
     CDS             complement(68338..68814)
                     /locus_tag="PC479_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2803286.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(68848..69426)
                     /locus_tag="PC479_000704"
     CDS             complement(68848..69426)
                     /locus_tag="PC479_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2803287.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(69530..72112)
                     /locus_tag="PC479_000705"
     CDS             complement(69530..72112)
                     /locus_tag="PC479_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2803288.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
BASE COUNT        22473 a        13546 c         7867 g        28340 t
ORIGIN      
        1 ttttattaca cttaatcctt atttttatca ttctacaaaa caaaaataaa aaagaataag
       61 ataataaata aaatacttat caccttattc tttatttaat tattttacaa cgaaaaatct
      121 aaactacttt ttaaatagct tcctcttcca tcaatttatt tgtatattac tgatttaatt
      181 cttcaagtaa ctgattggtt cttgcaatac tttatcctaa actctggaaa ctttgtaaac
      241 tacctctcta gcatttggta aatttccatc tggaatacct acagcaatac caaatggtac
      301 agaataccct tctggtatat caagtagttc tctgtatttt ttatttgtat caaacttcat
      361 ctttattgac tctgattctt ttacatcttc tttatccaat acactcacaa gaccttcaat
      421 atccccttgt tcaacaggtg taaataaacc tctaaccatt cctattatac aacttccaag
      481 ccctagtgct tgtgcagctg ttgttatatt ccctgtaagt attcctatat catatggagc
      541 ccatgaacta ctttcatcat aagaaataaa taaaacggtt ggagcaccat aaaataactt
      601 aaagttttta tttatctcaa cattactcac ctcatgaatc ctagataaag tatcatcact
      661 aatctctttt aaaagtttct ggtcttgcac aacagttatg tgccaaggct gtttattaca
      721 tcctgaaggt gctgcaaatg cacagtgtaa taatgtgttt acttgctcat cactaatttg
      781 ttctgtcgta aattttcgta tacttctacg actttgtatt aaatttattg tttggttgtc
      841 ttgaaaatta ttattcatat ctattcccct ttttatgtat ttcttagtac aaatactaaa
      901 tcttcagaaa attaatctta taattaaatt tctttacaat attattataa ttatttttag
      961 cttgtatgtg aagacttaaa aatataaaca cttttaatat atctcaatta tatatataaa
     1021 tgaaaatctg gtattcgtca tctatagaaa ctgctctttt ctatagttta atcaatacca
     1081 gattaataaa acatgatata ttaatttaaa ggaccccatc cctctttaga atgaatctct
     1141 gtttttacag acttaacact atctgtgtca acatcccatg acctacctgc aacagaaata
     1201 ccatttttac ctttatacca tctaccttca tcataatcgt aaactaggga agtataccaa
     1261 ttacccccag tcttattatt tgcaaataca actacagtag catcagattt agtaggatta
     1321 tatgcatatg gttgtatata tagtgtatcc ttacttaatc catattttgc cttaaattca
     1381 tctggaattg taggccaccc atttttatat ttattgtcta ataaatattt tctaaaatca
     1441 tcattaccct ttgaggctcc tggtattata gaagggtctt gtgcaaatga tgcaatcaaa
     1501 tctttcatac tttgatgtat atccctagcc atttcaacac catctggatg ttttgtacaa
     1561 gtaacataca cttttgcaat gctttctatt ccagttattc catcataacc atcatcaaac
     1621 gttgctgaat atattccacc tgacttacac tttggttctg tttcaaagta cttaccatct
     1681 ttgttttcta atacctcttt catgacttca ttcttgcctt tacttgattc ctcagccatt
     1741 gcaataacaa tttgagtctt tatattttct ctattagaaa gacatgtaac tgctttactt
     1801 ttttctatat ttctaaacaa tgctggaaca gctactataa ctaaaatgcc tattatagat
     1861 ataactacta acaattcaat tagtgtaaaa ccctttttat tcataataat cattcccatt
     1921 aaataaaatt atagtactta tttttaaata ctacatatta ataaattgtc tacataaatc
     1981 ctttcaacag aactactaat atacttacag aactactaat atactcaata ttcaatttag
     2041 taattaaata acttgtatgc taaaactcaa tatcctctac ctctattttg tcaatagcat
     2101 cctgtactaa tttttcaatg tcaagtgctg tttcagattt ttctattttc tctaatctag
     2161 gtttagtgac gggctttcta ggagaaaata cactacaaca atcttcttca ggcactatag
     2221 atgtttcaaa agtccctatt tttttggcta tatcaactat atctgattta tccattgcta
     2281 taagtggtct aaacactggt aagtctacaa cggcattagt acaagtaagt ccttgtatag
     2341 tttgagatgc aacctgccca atactttcac ctgtaattaa agcatcacaa tgtctttttt
     2401 cagaaagtct ctgcgctatc ctcatcataa atcttcttga aagaatagta gattcttctt
     2461 cattacaatt ctcgccaatt gctttttgta tttctaagat attaacttta tgaagcctta
     2521 ctctaccaca gtattttgcc agtattttag ctaaatcctt aactttttcc tgtgaccttt
     2581 cacttgtaaa tggataacta tgaaagtgaa ctgcctctac ttccatacct ctttttgcaa
     2641 ccatccatgt agctactggg ctatctatac ctcctgataa aagagacata gctctaccat
     2701 tcgttccaag tggcaatcca ccatatccag gtatagtatc actatagacc atagtatgaa
     2761 attctctgta ttcgcacttt attttaactt ctggatttct tacatcaaca ttaatcctat
     2821 ctcctacctt tgataataag tatccaccta tatccatact catttcttga gaagtcaatc
     2881 cgaaagactt atctcctctt cttgactcta ccttaaaagt tttatatcca gcctctattt
     2941 tttcttctaa catttttaaa gctatttctt ttaatgtatc ataatctttt ttagctctta
     3001 ctccagggca cacacccact atcccaaata cttttctaac ttcttcaatt acttcttcat
     3061 aatcatagtc ttctaaatca acatatattc ttccatactc tttatataca ttaaatcttc
     3121 caattggttt taacatattt cttatatttc ttataagcct attctcaaat atatatctat
     3181 tctttccttt tactccaatt tctccatact ttactattaa tatattatac accatttcac
     3241 ctcttaaact aatttattat cactatttat acagtattct ttattttctt ttcattattg
     3301 accttaagtc acaaattgat tctttcaaaa ctttcacagc ttctaacatt tcttcttctg
     3361 tattcatatc agataaacta aatcttattg ttccatttat ttcttcattt gtaagaccta
     3421 tagcatttaa tacgtaacta cctttctttt tagaagaaca tgcagagcca gttgatacat
     3481 aaataccttt ttgctctaag taatgaagta aaacttctcc ttttgtccct ctaaatgata
     3541 catttaatat atgacatact ccgtcttctg gagaattgat ttttatatct tctatattat
     3601 caattatttc attttttaat atatttttta agttatctac tttcgaaata acagcatcta
     3661 aatctttgtt tagtattcta acagcttctc caagaccata tattccaggt acattttctg
     3721 ttcccgacct aattccaatt tcctgaccac cacctgttaa cataggctta aggcgattat
     3781 tttcttttat atacataaat ccaattccct ttggaccatg aaatttatgt gcacttacac
     3841 tcataaaatc tatattgtat ttagatggtc taaagttaat ttttccatat gactgaatag
     3901 catcaacatg gaaataaatt ttttctttta aacccttcaa atactttcca acttcaccta
     3961 ttggctgtat agttcctatt tcattgttaa catgcatcat gcttactaag catgtagttg
     4021 actttatcgc attttttaaa tcctctatat ttattttccc atcttttccg acctctaaat
     4081 aagttacttc aaaaccatct tcttctaaat cttttaatgt atttaaaaca gaaggatgtt
     4141 ctatgttagt agatattatg tgattttttc tttttttatt taaactagta atacctctta
     4201 ttatagtatt attacactct gtaccacctg atgtaaaata aatctccttt tctttcgctc
     4261 ctagagattt agctatatct tgtcttactt cttttatagc tttttcaact tctacacctt
     4321 ttctatggac agaagaagga tttccatatt ccgtattaag tgcataaacc attttatcta
     4381 taacttcttg ataaggtttt gtagttgcgc tattgtctaa gtatatttcc aacttaatta
     4441 cacctacctt tatttaaaat cttcaactct aactattata ccactaatta tagtattttt
     4501 tttgttaatc tttattaaaa agacaatctt aaattataaa atattatttc atgaaaagct
     4561 agttttttca ctttattaca aatttttata gtaaaatata atatacttat actataccct
     4621 acaaaaatat attataaaat acaatatatg ataaaataat atattatata aaataaatat
     4681 attatattat acatatactt atttttacta taaaagttaa atttataagc ttacttgtta
     4741 taaaaataac ttttataatt tatttcaaaa aaaatattga tttttgtgtc gcattttgtt
     4801 ataataaatt aaggttatcc ccctggtaac cctagttagt taatatatct attcccaata
     4861 ccctttcata aggatatatt ttatatttat taaaaatcgg attagtaacg gaaattaatt
     4921 cggtggaaaa aaagagctta ccccgagctc ttttttttct gcaaaaaatc gtaaaaataa
     4981 agttaatatt aatataactt taattttaaa attaatatta accatcacta aaaaaggatg
     5041 tattgaattt ctaatatcaa taacatcctt tttatatatt atagcacttt tgctaaaaat
     5101 gcttttgttc tttcatgttt tggatttccg aaaacttctt ctggagtatt atcttcaagt
     5161 atacttcctc catctataaa aataactcta tctgcaactt ccttagcaaa tcccatctca
     5221 tgagttacaa ttgccattgt cataccttct ttagcaagtt cttttataac atccaaaact
     5281 tcgtttacca tttctgggtc aagagcagat gttggctcat caaacaacat catatctggt
     5341 tccatagcaa gtgctctagc tattgctatt ctttgctttt gtcctccaga taattgtgat
     5401 ggatatgaat cttttttatc taaaagacca actctactca acaaaatttc tgcttttttt
     5461 tctgcttctt ccttagtcat cttttttaat ttaattggtg ctaatgttat attatctaaa
     5521 atagtcttat gtgggaataa attaaagttc tgaaacacca tacctatatt ttgtctaacc
     5581 tcatcaatat tagttttttt atcgaccaaa ttttttcctt caaatattat ttctccacct
     5641 gtaggtattt ctagtaaatt catacatcta agaagtgtac ttttaccaga accacttgga
     5701 cctactataa ccattatttc gcctttagcg atttctaagt ctatattttt taatacattt
     5761 aaatctccaa aagatttact taaatttttt attgtaatca tattatcact ttccttttta
     5821 ctattctatg ttttccctaa atgctaaaca tgctctcatc tagttagaca cagatagttt
     5881 cttttctaat aatccaacta atcttgatag agtaaatgtt ataacaaagt aaattgctgc
     5941 tgctactatt aatggctcta atgctctaaa tgctgcattt tttactatat cagcagtacg
     6001 cattatctca acaactccta ttacagatat tattgaagat tcttttacaa gtgtaataaa
     6061 ctcatttgct aatgaaggta atatattttt tatagcttga ggtattatta tatatctcat
     6121 agttgaccaa taatttaatc ctaatgatct acttgcttcc atttgtccat tatcaactga
     6181 ttgtatacct gatctaagta tctctgccac gtatgcacca gagtttactg ataatgcaaa
     6241 tgcactagct ataaattcac ttggtatacc aaacaacata gggaatctta ttcctaattg
     6301 aggtaaacca aaccatacta tatatatctg tactaataat ggtgtccctc ttaaaacctc
     6361 aatatagatt gatgatattg ctcttaatac cttgctcttt gatatttttg ccatacagat
     6421 aattatacct attataaatc caaagcataa tgccacaaga gaaattgata ctgtcacacc
     6481 tgttccttcc aaaaaagaag tcccaaatct acttaaaaaa ctaaaatcta aactcataat
     6541 ttcatctccc acataacttt cttactaatt tgaagcagct tcagtagatg cttcatttaa
     6601 aaattcttct atcttcttgc catcttgtaa ttcttttatc tttttgttta ataattctat
     6661 aaaatctttg ttattgtcag attttttgat tgctgctgcc ataccttctg ttacatcttt
     6721 cccaacttca gtatttgcca tttttattcc atcatatttt ttaacattta ttaaagaaac
     6781 tgcttcatta gtaacaactg catctatgtt tccatttttt aattctgtaa ttaaatcagg
     6841 tattgctttc aaagattttg tagttgtcat ttttaaagtg ttaactacat attcttcttg
     6901 gatacttcct gcttgaactc caactctaag tttctttaaa tcatcttcag tttttacttt
     6961 gtctatatct gcatctttaa ctataactgc atttctactt aaataatata attctgaaaa
     7021 atcaacactc ttttctctct ctggtgttgg agacatacct gcaagaacaa tatcaacttg
     7081 gtctgcattt aaagcaccta ttaatccatc aaatgacata tccttaattt caacctttac
     7141 accaagctct tttgcaaatt cttcagccaa cattatatcg aatcctttta ttgaatcttt
     7201 tccatctact accttatgaa attcaaatgg aggaaattct gcactagtac caacaactaa
     7261 ttttccattg tcttttattt gttctaactt tgttttttct cctccaccac tacatcctac
     7321 tagcgataat gtcaatccta atactaatcc taaacttaat aattttttaa atactttcat
     7381 tttaactacc cctctctttt aattataatt tccctcagat tttaatactt aaatgtttcg
     7441 agttacaaaa aaagtctctc agtctttaat gactaagaga cgactttacc gcgttaccac
     7501 tcttattgat aaatacttat attactttca aaatttgtga ctgttagcat ttaagctgta
     7561 tggatacttt tatttttaaa tcaaaaaagt cccttagctt tacagctaag agacgaatta
     7621 tatccgcgtt accactcttt ttgataagta agtacttatc ctctcaatcc cttaaggcgg
     7681 gtaaaccaat tatcatacta aagtttctga taatctcctc cagagctctc ttcacataga
     7741 cttcattgct aggcttacac caacccctaa ctcgctgtaa atccatatat gctactttct
     7801 ctatcattgg atttgtttcc ttaaattgtt aatacaattg tacttcctta taatattttt
     7861 gtcaataagt tttttatttt tttaataatt tttacttgta aattatctaa atactactgt
     7921 ttatatttat tgatattagt tttgtaatta taaatagtat atttcatatt tttagatatt
     7981 tcttaaataa tactaaataa tatctttcac attgttgctt tttcttaaac aatactaaat
     8041 aatatctttc acattgttgc ttttcttaaa ttacactaaa taatatcttt cacactgttg
     8101 ctttttcttt aataatacta aatagtatct ttcacactgt tgccctttct ttaataatac
     8161 taaataatat ctttcacatt gttgcttttt cttaaattac actaaataat atctttcaca
     8221 ctgttgcttt ttctttaata atactaaata gtatctttca cactgtttct ctttctttaa
     8281 taatactaaa taatatcttt cacattgttg ctttttctta aattacacta aataatatct
     8341 ttcacattgt tgcttttctt taataatact aaataatatt ttccacattg ttgctttttc
     8401 taaacaacta taaataatat attaaaccct tatttctttt tctaataagt taaataatac
     8461 attttacaca tatctatttg tcttgcacat aataaataat acattatatt tctatctaaa
     8521 tttagattct aaattaaata gtataaatta tattcttcaa taaacaagtt ttcaaatcca
     8581 agattcgctc tctatcttta aactttattg taaacataat tccatctttt ttaattattt
     8641 ctccttcatg aaatttttta tggtatacct tatcccctac accaatactg tcaatttctt
     8701 ttttcgttgg agattttata tcattaataa ataatgattt atcaactttc ttaccatact
     8761 tatttaaagt agagcttata cataactttt cttcagctct tgtaatagca acatacataa
     8821 gtcttctttc ttcttctaat tgttcttctt ttttctcttc cttacaaata tcatatgatt
     8881 tttcatgagg tattgttcct tcatttacac ctataatata aacatttcta aattctaatc
     8941 cttttgcact atgcatagtt gtaaatatga caccctcagt ctgcttattc ttgttatttt
     9001 ccgttaactc agtctttact ctatcaatat gctctaaaaa ctctgtaaca gtattaaaat
     9061 tagttgctga actctcaagt tcatttaata tctctacaag tccattagtc tttatctttc
     9121 tgttgctaca ataatctaaa acatatctat cataatcaag actagttcta atataagata
     9181 ttgcattttt aggatttaaa gtatttaaat aacttaaatc tatttccaaa tcatttattg
     9241 ttttaacttg tttaggatga agattacact tatttattaa tgctgttata aagtcttttt
     9301 catccttaac catatttata ctgtctttgg atatatatct aaaaggcttg tttataattc
     9361 tcaaccaatc tttatttgat tttggattaa tacctattct taaataggct agtatgtctt
     9421 gacttgccca atggtcatat attgtaatta ctgagtcttt tactacaaat ggaatcctca
     9481 tatccataaa tacatctact aatgctcttg actgtatatt agttctataa attactgcaa
     9541 aatctgaata ctctacataa tctttttgta tttcgtcaat tatttccctg gcaatatata
     9601 aggcttcttc ctctgaatca tgcggagaaa tataggtaac acttcctcca tccccctgac
     9661 tacacttaat gattttttca tatctatttt tatttttgtc gataagtcta tttgcagtat
     9721 gaactatctc acttttagac ctgtaattta tatctaatat tattttttta gtattattaa
     9781 agtattgttc aaattcaagc aaaaaatctg gtctagcacc tctaaatcca tatatacttt
     9841 ggtcttcatc tccaactgca aatatattgt ttaatgggct acaaattaac tttaaaactt
     9901 caaactgtac tttatttata tcttgaaatt catctatcaa tatatattta tacacatttc
     9961 taaccatttc tagtgcagac ttattgttca aaagtaaata ataagttctt ataagcatat
    10021 catcaaaatc tattttatta actttagatt tttgttcttc gtataagttg tatactttta
    10081 aaaactcatc ttttgttaat acttctgaat taaattcatt tttatccata agttcatttt
    10141 ttacatagga aatttcattt ataacttgtc ctatattctc atcatcatca gcattctcta
    10201 tatttaaact ttttaggatt gccttgattg tcattcgctt tgacttttca tcaaatatac
    10261 tgtctaagtt gtatctttca aagtatctta gaattttaaa aaatacagag tgaaaagtac
    10321 cataggttac cttattaagt cttatgtcat cacttaaatt caaagctcta tttttcattt
    10381 ctattgaaga tgccttggta aaacttattg ccaagattct tgttggtgct atgtttttgt
    10441 ttacaaccat attggctatt ctatatgtaa ttactctggt cttacctgaa ccaggccctg
    10501 caagtatcat gcaaggccca tctatatgct caactgcttc taattgattt tcatttaact
    10561 ttcttatatc tatatttgtt gtgattatta gctccaccac cttgatttat aattatatta
    10621 ttttcttttt aattataaac taaaagttca taaaaataaa tttaattttt cctctattaa
    10681 accacatcat tcctattttc atgacatatc ttgagttctt ttttaatttt gtcgcttata
    10741 tcttcaggta taaatgatgg tttactaata tagtagcctt gcatataatc tactccaaga
    10801 ttaattagtg tttccatttc accttttgtt tctacacctt ctgctatcac tttaatattt
    10861 ctttcgttag aatatgagat aagatttttt aatattttct gtctgttttc atctttatca
    10921 atacctctta caatagacat atcaatcttt acatagtctg gcattatata taatagtaca
    10981 gcatctccgt tgtaacctgt cccaaagtca tctaaagcaa tttctatatt ccaatggtct
    11041 atatatgctt tctttttctc aataaaatct tcattaatct tatcattctc tgttatttct
    11101 agtactactc tatttaaata ttcaccatat aaatcttcaa tttcctcaaa atctactttt
    11161 gacaaattat tattaggaat agaatttaag aacagtttgc aatcgccaaa tttttctttg
    11221 tattttttaa atgatttaag tgctttaaac catgtaatac gctcaatttg atataattta
    11281 gactgtgaac gagctaatct aatgacatct attggcgaac taaaagtttt tattttaggt
    11341 ctcataagtg cttcatatgc aaaaacttta cctttacaat ctacaatagg ctgaaatgca
    11401 tactcaacta attgaccatc aattaacttg tttaattctt ctctattact caataagaat
    11461 gaatccttat tatatatatt aatatcaaat tcactaactt cacccttgat tgtattttta
    11521 atttgataca tagcaaaatc tgcgtatctc attaattcat catagttggt tgagtcatca
    11581 gggtaccagg aaattcctgc tgatgccctt attttaaatt ctgttccatc atgaagtttt
    11641 aataaggtat tattcatctg tttcttaaca gaattaataa tatctcttat actatcttta
    11701 ctttcatatc catatataaa tacataaaac tcatcacctg aaagtctaga tactatacca
    11761 ttccatgata taaacttctt aagtatgtct gcagcacacc taatatattc atcaccagca
    11821 tcatgtccat atgtatcatt aatatatttt agattatcta aatcccacat aataaatgct
    11881 gctatcttaa gttcatcttt attcttaaat ttttcttgta tctgtgaatg aaaagctctc
    11941 ctatttaaaa gattcgttaa aatatcaaaa tctctttcat attctatctt ccttttctca
    12001 ataaattctt ttgttacatc aacaacaaca ccaagaacct ttagctcatc ttcaaagata
    12061 ttcattctta tccacttaag ttttttatct ttagattcaa acttatatat atatgtattt
    12121 tctgtttcat aactttcttg caaatattta tctagtcccc ttaatttttt caggaactta
    12181 tttttttcca tatatccata ttcttcaaat ttgatttcag tagtaaacac gctaaaaaat
    12241 tcttttgtac aaaaaacatt ctctgcgttt ttgtataact caaaagctcc aattggcata
    12301 tctaccattt caataatctg tgataatttt gacgaagaat ctgcaacatt tgcactgagc
    12361 aactctatag ctgaagataa ttcatctatt tcagatatat ttatcttatc aagatttact
    12421 ggttttgatg gattactgtt tctaactttg ttggctaaaa gaacaattgg ttttgtaaat
    12481 aatcttgatg caatataaat acctattaga cctattaata aagaaattgc tatagatatt
    12541 ataactaatt tctctaccct ttttgaaaat gacaaaagac ttttttcttc aataataccc
    12601 aaaagagccc aacgttcatt ttcaaatgga gtattggagt tatataaatc taagtattgt
    12661 acactagcat aaatgttgcc attaccacat tcattatctt tatttttaat cttatatata
    12721 tttttatacg attccttctg ctctaaatta gaatgtaaac catcccaaaa tttttctttt
    12781 aatgagtatc catttgaaat tacattttca aaggccatat cattatttct atctacacct
    12841 aaaatataat tacccttttt attcccattt atctctgagt atggaagtaa ttctcttaaa
    12901 taatttacac ttaaatctat tccaagtact ccatatacag ttccatcttc ataaataagg
    12961 ggaactgtat atgtcataag cctatcacct gcatttgtca ataaaaactt acttccccag
    13021 tatcctaaat tagaacttga tacatttgga ttatcaattg cagctctaaa tggtttataa
    13081 aaaaaatcat ctccatcttg ctcattttca cttttaaatg aaaacttagg ttcccaataa
    13141 ctgtccatag atattccaaa acttcttgct atatctgatg aacctctctc aattaacaaa
    13201 tcagaattat catttggatt aaacttaggg tctaaatccc ttatatacaa tcctgtccta
    13261 cttatttcac ctttactatc tattgactta tcgttaatat ttttgtcact taggattata
    13321 aaagctcctg taacagaatt ttttctcaaa agatatatta aatcatttga tatgcttttt
    13381 ataatatctt tgttaatttc ttcattgttg cctacatcct caattaaggc atttttttca
    13441 tttaatactt tttttacaga ggaattaatt gtagcttccg tttcacttgt atttgaccat
    13501 ctttgtaaca tttcattttg tatataattt ttacgattaa ttactctttc atttaagatg
    13561 tcaaacgaat tatcatttaa tttcttaata gttcctcccc ataaaattgt ggcacaaaat
    13621 aaacttgttt gaattagcat aacaataatt aggggaaata tcatcttttt cattattgaa
    13681 ttgttttttc ttagattgtt ttccataccc attccaccta tacctttgtg ctcgtcaaca
    13741 cactaactgc caattcatct atttttttat agcattttca agctcagcct taaaactttc
    13801 aaaccaagtt ttaaaatttt tatcattatt aaattgtgct actgcatcct tcttagaagt
    13861 tccacttttt actaattcat ttattttatt tgaatcttct ttagctttat caagcattga
    13921 atattcaagt atttgtctag ctttagttcc accatcaaat gctttattag tatataaatt
    13981 acaactgtta atctgttcaa tagatgaggc tattgaacct tcaactttat cagacaaact
    14041 caaattatct ttcttcataa cttcttttat aacatccaaa tcattcgttt ttttctttac
    14101 tggtaagtat ccagaagcaa tagagaattt tatattaggt tctttatctg taaaccattt
    14161 taaaaattct actgatgcat attcttcttc tttagtagat tttgttacag ccattcctgc
    14221 accttgttgt acagcatatt ttccttctcc tttaaaacgt ggtgcttgaa aaacagatgt
    14281 ttcaatatta taactctcat tatcatttag cattactcta tctggaaagt atgctgctcc
    14341 tgaactagaa cctacaagag ctaatatatc acctgttttt gcatcatcag aacgaaactt
    14401 accataagaa gcaaaatatc catttatata tggtacatag aaattatccc atagtttttt
    14461 cataactttt tcatcaatat ttagcgtaac tttaccatct ttaacagaaa aaatttctac
    14521 tcctagttgt ttacttccta taataatata atttgctaaa gcatctcttc caaaaaatgc
    14581 tttaccatca ttaggctctt tcgttaaact atctgtccat tcataatatt tttttgatgt
    14641 ctctactaat ccttcaattg tttttatatc atttatattt gaatttgtag cttttgcaaa
    14701 tttatcccaa tcagttttat ttaacatcaa aatttcagtt gattttgcaa ttgggaaaag
    14761 tttaaactca tccttagaat ttattctgcc ttcttctaca tattcgtcta tgtattcatt
    14821 taattcttct ttacttatgt acttatccat atctactagt aaccctaatt tatccatttc
    14881 atatgcagta tctgagtatg ctgcaaatat atttggtaca tcttcagaac caactttttt
    14941 atttgcagaa tctaaaactt tctctgataa atcaactaca ttaccttgac tgaatgcttc
    15001 aacaataatt cctttttcag cacctacagt gtcattaaat tccttaacaa gcttatcaaa
    15061 agccattttc tgctgtccat tataataatg ccaaatttgt attgatgtcg gattttctgg
    15121 gtctaactta taattatctt ccttctttaa actacatcct gtaagtaata aaacaatcat
    15181 tgatgtgata attaattttg caaacttaca cctattcata aaatctactc cttcaaatta
    15241 taaaatttat aatagcatta aaactgtaac ttcactattt atattaatta tagaacgttt
    15301 attgatatat ttaaacagga tttacaacat atttaaaagc aattaagaaa catactatta
    15361 caaatataga aaataaagct tttacataat ttttatttct aaatgcacca aatgacacca
    15421 atccttgttg aatagctact gtaattacta aaagtatcat tattatatta agttgaacca
    15481 gtttaaataa gtaaagcaat gaaaacacta ctagaagtac acataataca atactagatt
    15541 tattaataat atcatcaatc ttatctactt tcttagattg cttattaatt ttatttaatt
    15601 tttctttatt taaattctca acttttttat tatacctttc tcttgtttta tctttatatt
    15661 tagtattttt atttttattt cccatttatt ttccatcctc tataaatttt ttaattgagc
    15721 ttatacatta tttaaatatt atatatttaa gataccatat attcctacaa aaaggtaact
    15781 tactatgtat ctatttttga ttttcgcata aaaaagccat ctcctttaga attaattctg
    15841 tattgagaca gctaatttaa ctatttatcc tttgaaacat tttctacatt atcatattta
    15901 ctatcaacta aaactatatc aactcttcta ttttttgacc taccttcttc agatgaattt
    15961 tctgctattg gtctaaactc accatatcct acagcagata atttatctgg tgctatacct
    16021 gcattgtcaa taagaagttg aacaacattg gttgctctca taactgataa atcccaattt
    16081 gacttaaact ctgaattttt tatagacata ttgtctgtat ggccttctac tctcatgtaa
    16141 ctattcattt catttaacat cttacctatt tgaataatct tatctctaga attatccttc
    16201 acaatagctt tccctgtatc aaatagtatt gtatctttaa ggcttattac taatcctcta
    16261 tcttgaactc ttaatgatac tgaattagcc ataccatttt cgtttaaata cttctgaatt
    16321 tcttcaccaa tttttttaaa cttagcattt tgtaaagtat ctaaatcatt acttcctggt
    16381 tcaactactg gttctatctt gctattacca ccttcaataa ctccagaaga accaccaaaa
    16441 gcagaattta gagattgaga taattgttta tatttctccg catcaacatt actcatagaa
    16501 tacattatta caaagaaaat cataagtaat gttatgaggt ctgaataagt taaaagccat
    16561 cgctcattgt tttcttcttt ttcatcttca tcatacaaca taaacattcc atccttttat
    16621 attattcagc tctaccacca atttcttcaa gttcaattag ttcttttgaa tttaaaaata
    16681 tttttaattt ttctgtcatt aaattagaat tatcaccctc aagtatggat aatattgctt
    16741 ctatcaccaa aagtctttca cttacttctt gttgatttat agccttaagt tttgatgcta
    16801 ttggtaacca tattaaattg gcagaaccaa caccatacaa agtagccaag aatgcaacag
    16861 aaatttgagg tcctaatgca tctggattac ttgaaagatt acctaaaata tgtactaatc
    16921 ccataactgt acctattatc cccagtgtag gtgcatatcc acctgctgaa tcaaaaattg
    16981 ctattccttc tttatgtcgt tttgatgtta tctgtatctg tgattccaat atatttctta
    17041 tagtaactgc atccactcca tctaccataa gttcaagccc ttttctaata aaagggtcta
    17101 attcatcatt ctcacttatt tctttgtcaa tactaagaag cccctctttc ctagcctttg
    17161 ttgcaatttc tttgaaatag attatatttt ctatgttatt aggcttttta tttttaaaag
    17221 ctaataacat tatctttata gactttttaa acacagggaa tggagtcgaa gctccaacag
    17281 ctcctatagt tccaccaaac acaacaagtg ctgatgaaaa tacaaataat gctccaacat
    17341 gtcccccatc aataacaaaa gcaactacca aagatgctat ccccactatg aaaaaaatta
    17401 aagttgtcat ctattccctc ctttctttag ttatacatat ctaatcattt atgacattat
    17461 aaaaagacta tctttaataa aaatagcctt taataattgg cggagaagga gggattcgaa
    17521 ccctcgcacc ggttacccag cctaatccct tagcagggga tcctcttgag ccacttgagt
    17581 acttctccat atctctatac taataaatta tataataaaa tcatatttta gtcaattgtt
    17641 ttataaaatt tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg
    17701 taagttttaa atatggtaaa attatcttgc ataacactta taatattatt tgataaatat
    17761 acatacatta acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa
    17821 taatactagt ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa
    17881 gtattctcaa atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat
    17941 caatagaaac tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag
    18001 tttatggtgg tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa
    18061 aaaaaataaa ctcaaacgat aaaaaacaga ttgctattaa atgtagtgag tttataaata
    18121 caaatgatac tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga
    18181 aagataaaaa gaatctaact gtaataacca actcaatttt agttgcaaac gaacttatga
    18241 atactaatat aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt
    18301 ctggtccgct tacactgtca gctattgaac aatttaatgt tgataaagct attttaggcg
    18361 ctggaggtat cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta
    18421 gaaaaaaaat gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg
    18481 gtataaattc atttgcatca gtagctccta tagaacatat agatactatt attacctctg
    18541 ctagtataga ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag
    18601 ctgataatta gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg
    18661 agccaaataa ttatttattt agctccattt atgtctaaac tatactttat attttaatta
    18721 tgtaactact ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt
    18781 ataagtttta gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta
    18841 tttaaactcc tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc
    18901 atctttcttc attttaatac tttgttcttc ttccaaggtt aaatcttttg tcataagtat
    18961 agcaagtgca attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat
    19021 ctctggaact actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac
    19081 cagccaagca gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac
    19141 tggaatacta ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa
    19201 ttttccaccc actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg
    19261 aaaagtacct aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg
    19321 tgtcatgcct tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc
    19381 tatcgttatc aatatagata taaattttcc aaaaattaat gacccattta tcataagagt
    19441 agaattaaat tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt
    19501 tataattacc attgataagt taaatcctgc tattaatcct cctataatac ctccaaaggg
    19561 tattgttatc aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat
    19621 aagacctagt cctactggaa ttgaaaatac taatgtacat ccaagcatgg atgacacaac
    19681 caatcctgca agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc
    19741 tcccatatca ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa
    19801 gattggtatt attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc
    19861 aaccattcct aaagccaatg gacccattga attaaaccct tcttcaaact gctcgccaag
    19921 tccaaattta tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat
    19981 gtataatata attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta
    20041 ttgtaactta attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta
    20101 ctgcttggtt ccatccaagt aatatttctt ctcttttttg agtagtcata tttggaatat
    20161 attttttgct tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag
    20221 aaagacctgc catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa
    20281 ttggtatacc taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac
    20341 catctactct caacattttt atttttttcc ctgaggctaa gttcatagaa tcaacaacat
    20401 ctttgacttg aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac
    20461 ttcctgttat cccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac
    20521 ctgcaaatgc tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat
    20581 cagtttctga ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac
    20641 ctgctatata cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa
    20701 cagttgtcaa aataccatta cctgctatat ttagttcctc acctatattc atatatagga
    20761 aacatcctgt accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata
    20821 aagctgctgg ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct
    20881 ccttatctgt agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag
    20941 gtactcctat aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca
    21001 ttgttcttgc tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata
    21061 ttaaccaact atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac
    21121 cttttacatt atctattacc catttaattt ttgtacctga gaaatatggg tctataatta
    21181 acccagtctt atctattatc ttattttcta aacctggtat attctttaaa ttttccacat
    21241 aatcataagt tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt
    21301 tatcccataa cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt
    21361 cattgattcc attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat
    21421 tccaaatttc tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt
    21481 ccatataatc gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg
    21541 ttgttccttg gtcaattgct aatacatatt tcactttaaa tgtcctcctt tattttcaga
    21601 attaataaat tcttttttat tttttgaata tataatctat tcaagttatt tatattttat
    21661 atcatcatgt ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt
    21721 gattgaaaaa gtatttttat tgaaatattg ttgcaatatt tattatttta ttctataatc
    21781 tagttaaaat aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa
    21841 atttaacttt ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag
    21901 taatggttac agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa
    21961 atgagtttat ggatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa
    22021 cttctaatta ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac
    22081 tatcttctat ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag
    22141 aaaggctaga agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat
    22201 gggcagaata cccggaccta gacattttag aaaaatacat aattgaaaac tctgatataa
    22261 aagtaattgc tatggtgttc catgaaacta gttcaggaat gataaatcca gtccctgaac
    22321 taggaaaact tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag
    22381 caggtgaata tattgatgtg gaagagttta atatagatat aataacaggt gttggtggca
    22441 aagctcttgg agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa
    22501 atattaaaga aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg
    22561 ctaaatcatc ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg
    22621 aagctttaac tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg
    22681 ctattttaag gtatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata
    22741 tgtctaatac agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat
    22801 tagggctaga aagggaaagt ggctatgttg tctatccagg aaaaggcaaa ttcctagatg
    22861 ctaacatatt ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct
    22921 tagatatctt aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa
    22981 tcctacaata tatcagtata aattaaatag actgattttt ttaaattttc ctataataaa
    23041 ttatatttta tatttttgta taagggtgaa ctataatatg taaagttcac ccctatttca
    23101 aatctttttc cttcaatata atctttttct tttaatttat tttatatttt tgacgcaatc
    23161 gttttttatt caatcatttt atattcttga tacaatcatt cttacactta attatttttt
    23221 gtttttgaca caatcgtttt ttgttgaatc attttatatt cttgatacaa tcattcttac
    23281 acttaattat tttttgtttt tgacacaatc gttttttatt cagtcattta atattttcaa
    23341 cacaatcatt cttacattct attattgttt gtttttgata caactgtttt tattcagtca
    23401 tttaatattc tcaacacaat taccttttca ttcaatcaaa gtaatcttta agcattattt
    23461 ttcaagctca tcaaagtaat tatatacgat aacctcacct gtctctttat ctgtagtctt
    23521 tataatgccc ttagctccat ttacctcttc ttttgtaaat tcatcatcta acacaacttt
    23581 gaatttatct tcctttccat aaccttgttg aaattcatct ctcaaaattg catttttttc
    23641 tgcttttggc ttcaactcaa attcaattcc agatttcatt tcatcaaaat aatttaatac
    23701 tacaacctca ttattcattt tattagttgt ctttataatc cctttagctc catttacttc
    23761 ttcttttgta aattcatcat ccaatgttat attaaatttg tcttcttttc catattcctc
    23821 ttgaaaatcc ctcttcattt cattgtattt agtttcaaac tctttactct tgtttaattc
    23881 catagctgat acatttgtca atgatgctcc aattgccatt actgttaatg ccattactat
    23941 accactttta aattttttta ctttcatatc ttacctccta aattaaatgt tctcaatgaa
    24001 attatataaa gaaaatccta agttttacag tttcattctt gtaactaaaa ttactattat
    24061 gtaaccaaac tttttgcttc aactattcct taaaagttgc ttttttgagg aatagttgaa
    24121 gttaaataat tagattttat ctatatttat agatttaaaa tcaaatttat ccatataaat
    24181 aaatacttat ttttgcaaaa taaaaatttt taattactcc aatccttctt atttacttaa
    24241 aatcctatga taagaaattt aaaagtcttt accttcttta acaataattt atttactttt
    24301 aacatacaaa atatagagag tatatcaaaa ctattttact gaatattcta aaaataagta
    24361 ccccctatta gtatatatat ttacatatta aaaaaaagtt ttaaataaag ttatattttt
    24421 tacaacagtt ttgtaattaa aaacatattg aaggtaaatt taaaggtact atatactata
    24481 aatatgtcga cacaataaat ttaaaaagga gaactaaata tgaaaaaaag attaataata
    24541 atgatattgt ctgttgtctt agtactatca agtatactta cgatatttgc atattcaaat
    24601 attaaatata ataataacaa attgatatat tctaatatga tagataaaaa aactcaaaat
    24661 agtgtaaagg aaatcctaaa agaaaataaa attaatgaaa aagacataga tacatttatt
    24721 aaatcagtca acaattataa taaattgcaa gttaaaatac ttcaaaataa tataaatata
    24781 tctaaatctg gatattcttc aatcaatgca aaacaagttc catataacct agaaaaattg
    24841 caagataatt gggtgaaaaa attccctgat tatatggatg taaattgtag aataactgct
    24901 tttagacttt ttaaagattt tataaattca aataagaaat ttactggaga ctctattgat
    24961 ttgaatgtcg acttggacac tataatgaat aataaagatg ctaaatttag caccaaagat
    25021 gtagaaaaat ttatcaactt cttttctgca ataccagcta aagatacaga tgatacaatc
    25081 aaaattgcag aacaaataaa aaatgaatgg aaaaaaagaa aaatatcttt taaaaataat
    25141 aaaaatatat ctattataaa tggatttctt cgttatccag aaactaaaaa tgtatttata
    25201 ggtcatacag gcatatgtat taaaactaaa aatggcattc tatttttaga aaaatacggt
    25261 gttacatcac cttatcaagt tactaaattt aaaaataaag aagatgtaaa aaattatatg
    25321 tttaatagat tgaagatgtc agaaggtgaa attgaattac ctgaccctat aataatggaa
    25381 aacgataaac ttatgaaata ataactttaa acgcaaaata gtatttataa ttactaagtc
    25441 aatataatag agaaggtcta tagatttgtg taaatacaag cttatctaca atcttatata
    25501 agaatgttta tataaataag atagattaat tctaagtcct attattctat tgaattttat
    25561 tattatttta tttagattct attgttattt tacttagaac ttattattat tttgtttaag
    25621 ttctattact attttcaatc atataaattt acaaaagact gtacataaat ttattttata
    25681 caaacttatc tacagtcttt tgtgtgaata tatctaaata atatcgtatt tatttatttc
    25741 ctctgcacag tgagaatcag aacctaaaac taatggaata tcgtattttt ttaccaagtc
    25801 taataagtat ccatctatat aaggctcttt gcagtactct tttctatttc cagaaacatt
    25861 ataatcaagt tcataacctt tttctttaat taactttatt aactcttcaa gaattttatt
    25921 tcctttataa tcgtatggaa atacctgatt aaactttcta actaaattta aatgacctat
    25981 tctttttggt ttatgtatac ctaaatctga atttacagct tttatcaatg tttcataata
    26041 cttattataa acagcttcaa tacttcctaa tttgtcgatt aaattttgaa actcctctac
    26101 actgtaatct acacaataat aatcatcatc aatcttaatt atatgtactg ataaaagaga
    26161 atcctctaat tcctttccat atttatttaa attctccttt atcccttctt cgtatccttc
    26221 tataaaatcc acttccgacc caacatttat cttgatttta ccctcatact tttctttcaa
    26281 cttcttgaca tcattaaaat actttggtaa atcttcaata ctcatacaac tatcattttc
    26341 aggagcaggg tctctaaatc catttgcata cggaaaatgt tctgtaaatg tcatttctgt
    26401 tattccaaca tccattgctc tttgtacata tttttcaaaa tcatccttac ttccatgagg
    26461 acaataaggt gaatgtatat gaccatcttt cataaataat tcactccttt tttaaagaaa
    26521 aaatctaata taaatgcata aatcttttaa cttcaaaata atatttttaa tcatgctaat
    26581 ttttattata tctaatatta gagtaatttt aaactgcctt ttaagtatct tatcattagt
    26641 actattttca acttaacatt aatattattt tttaacttaa cattaatact atctttaaat
    26701 agtaatactc aatatataat tacttaacat aaaataactc aaattgcttg atgaggcttt
    26761 tgcagtattt aaagaatata taattattta acattaaata atccaatctt gtaataaaat
    26821 ttactcatta aagtatatta cagaaaatac ctcaaaataa actttttagt tcatgatata
    26881 ctcttttttg catgaaatta gatatatttt tatcatttca acaatgaaag ggaggcttat
    26941 atccatcttt agacaacccc ttagataaaa tttttaataa ttaaacatca caaaatataa
    27001 aaatcgctta tatcaaacgt ataagcgatt aattatattt acatatgttt tttagcctct
    27061 aacaacattt tattagcttc ttccatatac tctatagcct tttcaatact ctttgctgtt
    27121 tcttcttttc caatagctct agccttttca acccactctc taaatccttc ttcatgagtt
    27181 tcattgtgat ttatccaatg tactaaaagt atttttaaag ttttttcatc cttagactct
    27241 gatgattcct catgactatg ttcgtggtcg tggtcgtgag catggtcatg tggatgttca
    27301 tgacatatat ctccatgagt atgttcgtgg cagtggtcat ggtcatgatg tccatgatga
    27361 tatcttacaa tctctttatc aaaaatcatt agttattccc cctgttattt tatattaaac
    27421 tcttttttta taaggtttac agaatctttt acaagtagtg ctaatggttt accttctaat
    27481 ccaactaact ccacattttc tggcataata ggtaaaagta tcttaagtgc ctcactgtct
    27541 gcaatagctt cacttatatt acaagtaact tctcccatca tagaattagg tattattaca
    27601 gaaataggtg caactattat atttgctttt ttagctgata ctactatagc attttcccct
    27661 gtagctcctt tattagcatg agctttcatc atagaactag ttgctataga attagtccct
    27721 agtgcataaa tttctacata tgtaggtaac tcttctctca aagaagaaac tatctgggct
    27781 ccaatacctc cacccattcc atctatcact gctattatca tatgaaattc ccctattaat
    27841 taattttctt gtattattat cttatggtcc atcaatctta tttctttaat tatcccatct
    27901 attatctttt gctctcctaa taaatccgtt aaataaatct ttccatcaca agggtctata
    27961 ttaaccacat tttccataac cttttctaac tcattattac ttttacaaat gaaagctgat
    28021 gattcacaca tataaatctc ctcctaatat gatttttatt aaaaatcaaa aaaagattcc
    28081 cttaggtatt tcaatacacc aaagaaacct tcatgatttc agtttttgta tattattata
    28141 ccttaagtat atcactttaa tttatgtaaa gtcaattaaa gttaacgctt tttattatac
    28201 tcttcattct tgtatttttc atatagttct ttaactaatt gcttctcata atcagtcaaa
    28261 tcgccctcca caaactcaac attaaattgt tcagctaatc cctttactat gtttttctgt
    28321 agaatatcta tgtctatttt tctgccaatt tcattttcta ttccacttgc ttttttagct
    28381 gtaaatttca tagctctttc tttcaattct ggtgtcttag tttttattaa tttaaacaat
    28441 ttttctacat caaaatccag tattattgaa ccatgttgta ataaaacacc atcttttcta
    28501 ctttgagcac ttccaattac cttcttatta ttaatagtaa cttcatatga tgcatgtgca
    28561 ttaaaacatg cagctgataa attttcccta cttattctct cgcctctatt taaattatct
    28621 gtttctatac cacttagatt caacccttta actagacctt cacttatata tctataagat
    28681 aggttgatgc tcttatcgat taaaggatta tcttctccta taattatact ataggttagc
    28741 tcattatcat gtaatacagc tcttccacct gtaattcttc tagtatagtc tatattcata
    28801 catctacact tatctaaatc tatctcatct tccaatttct gaaaatatcc tatactcaaa
    28861 caagctggtt cccatgtata aaatctaaga gttggcttat tatgtccttt cttatatgca
    28921 gtaaatatag cttcgtcaat tgccatattc attgctcctt catatgattt attatgtata
    28981 actctccact gattcatgat tattacgcct ccatttttag tataaatata tcctagaaat
    29041 cctctataga tttatttctc ttattgctaa atctatctca ctttgattta aaggtcttga
    29101 atagttgtat atagtacttc ctggtctttc attatagtat ggtctattac aatccttaca
    29161 acctcttatt tcaaaaggat agcctttgtt tatgtctttt aaaatatcat tgtcaagctt
    29221 tatactattt aagtatccat ttttaaactt aaaatactct tttggataac ctttatttat
    29281 catataagac attaactgta cccttctata actttctatg cttggttgat ttatcttttc
    29341 cattttagtt cctctaactg gagtaaatgc aaataaacta atcgttacat cattttcctt
    29401 taaataagta tataaattgt atatatcttc atgactttca cccataccaa ctattatatg
    29461 tgtacttatt ttattgggat atgattttga catttcagtt ataaatttta acttttcatc
    29521 atagttattt ccttttattt tttcatatag ttctttattt gctgcatcta ttgctattcc
    29581 tattttatca actcctgctg aaaaaaactt ctttatctgt tcatcatttt ctaattttgc
    29641 agatagtgaa attggcatgt ctatcttacc acttatatag tttataaagt ctaaaacaga
    29701 gttatgtgct tcttcacttg ccattgattg aatacatatt ctctttatat tttttccttt
    29761 ataagctttt aaagcatcta atatttcttc ttttgaaaac tctggccaaa ttactcttga
    29821 taatttatct tttctagtac tactttctat tgattgagaa caaaaactgc atttatttat
    29881 gcatttttct ccaatcatta tatatgctgt ggtaggaggt atatcgcttt ttttgtttaa
    29941 tatcccaagt tctattgctg tcccaactga tagtctaatc ataaaacgca acactcctta
    30001 cactcattta ttgtcatatt catttctata gccttattct ttgcagacct tgatggtaat
    30061 actattctat tgactccaca cattatagat aattggtcta gctcttttct atacactcct
    30121 cttggtctca tacaacctag attaatttca gtatctggta aattaattct agcttcacat
    30181 aaaatatctg ctaccccttc taactcagga ggttctacat tttcatactc agtccctttt
    30241 gttggaataa ggactataaa tgtaagctta ttaggaggat ttttttgtag gtattctata
    30301 atttcatatt caccctttat ttgtcctcct tttaaaccaa tacatatatg tggtgctact
    30361 tctgtatgtt cttgtattgt attatagact tcaatataat cttctttact ctttttcatc
    30421 ttataaactt ctcttattgt ctcatcatca aacactaaat caaaagaaac tatatctaaa
    30481 tacttacaca actctacaat gctatcttta tccataagac ctaaatgagc attcaatcta
    30541 tatccttgct cttttaagtt tttaatggta ttaatatgag tatttatagg gacatctccc
    30601 tcataactac aaccgccact caatagaaag ctagttatat tccttgattg tactttttcc
    30661 tcatattctt ctattggcac catattgttt aagtagtgac cgttgcaatg ggcacatttc
    30721 aaactacatt gattgcttgt agtactaaga gctagtgtct ggttaggata agcaaactct
    30781 attttatttc caaaattctt taatttaata tcaaatgcta ttttcatttt ttcagataga
    30841 ttcttattca tagtttattc cttcttaagt acattttact cttctagtaa tttctttgct
    30901 gttgggaact gctctatatt cgtatgtggt aagaaacatg cagaaataaa ttcatccata
    30961 taacttggat atacactaag ttcaacataa gttatttgat tacctatttc ttcaagttta
    31021 ttttttgcat ctttacttat aagcgctaag taagaaccaa caagtgaact attccctata
    31081 taagtaaatt tttctctatc aatatcagga agtaacccta taattattga gttttctatg
    31141 tctaggttgt ttcctatacc acctgctata taaaccctat ctaatatact aaaatccata
    31201 cctaaactat ctacaagagt gtatgcgcct gaatatatag ctcctttagc tctgatgaaa
    31261 ttatctatat caacttcatt gactactata tcattttcta aatcaaattc ttctttaaat
    31321 gctaatacat actctcctat ctcatgctca ttaaatctaa ctctcttatt atctaaatcc
    31381 ctgtatattt tacctcttct atctattaca cctttagtaa tcatttgaca gattaaatca
    31441 attataccag aaccacatat accaactggt gcacattcat caataatctt taatgttggc
    31501 tctaaagtat ctttatcaat aattactttc tctattgctc ctgctgatgc tctcattcca
    31561 cagcttatcc ctccaccttc aaaagcaggt cctgctgaac atgcacagct catcatataa
    31621 tctttatttc caaatacaat ctctccatta gttcctaaat ctataaataa aacattttct
    31681 tcacttgacc atatccctgc tgataataca cctgctgtta tatctcctcc tacataactt
    31741 gcaacagatg gagctaagta cacatatgca ctgtcattta caaaaagacc aacattttca
    31801 cccataagtt ttggagattt taaaaatggt ggtatgtaag gttcttgtct taagaagtct
    31861 gtatacacac ccaagaataa acttgtcata gttgtatttc cggcagccac taatgtaact
    31921 acatcttcct tatctattcc atttctttca tatattgttt ttaatagtgg atttatagtt
    31981 tcctcaacta tagctttatg aagtgtctct aatccatttt tcttagttga atatataatt
    32041 ctatttataa catctgctcc atattttatc tgtgcatttc cagaagatgc tttatctact
    32101 acctcttttg agtataggtc tactaaacaa acaactactg aagttgtacc aatatctatt
    32161 gcaactccgt ataaggaatt ttctttattt ccttgttcta tatttatgat tgtaagtttc
    32221 ttttgttttt gaacatatgt tattgtaacc ttaaaatcac tctttctaaa tacagttggc
    32281 atttttctaa gtatatccaa tctaaaatct atttcattat atcctaggtt atttctaaca
    32341 tatctttcca atctatctac atcacttata ttatcatcta aatttggttc ttccatttct
    32401 atgtatttct tttttatatt tgttttaaat tgaaggttat gttcttcaat tattttttta
    32461 gctctttcaa aaatttccct atcctctttt ttattactac cttctatctt cataccatgc
    32521 atagaagatg atactttaga aggaacttca atttctatat ctgatattac ctttgtacaa
    32581 caagctagta tataaccttg ctcccactca tcatctgtta tatgtcttgt tttttctgta
    32641 tcaacttttc cattcaataa ttttacttta cacttcccac aagacacgtt tccattacaa
    32701 ggagcatcta tgaatatatc tgcatttctt gcaacctcta gtaatatatc cccctcatta
    32761 caataaacct ctttattgtt tggtgtaaaa cttactttta tcatgttatc cctcccaatt
    32821 tgttctatat atgataaaag gaaagtatag ctaaatttgc gtgcactcaa attttactat
    32881 actttcccca attataataa tatcttaatc tttaattatt ctaatccagc ctcgtattct
    32941 ttatcactta ataacgtatc aagttcagcc acatcagcta gttttacttt taatatccag
    33001 ttttcatatg ggtcttcatt tatacattct ggctcatctt ctaatttttc attaacttcc
    33061 aatacctcac cattaacagg agatattaaa tcagaagcta ctttagaaga ctcaactact
    33121 ccaaaatcta ctcccttagt tacagtatct tctacttctg gtgtttcaac aaacaatatt
    33181 tctcctaatt gatcttgagc atagtctgtt atacctatat atacaacatc tccatcaatt
    33241 acttttaccc actcatgatc tttagagtat tttaattctg gtaataattt catttcgata
    33301 gacccccaat attatattat tgattaaatt acttaaatca tattaatatt atatcatatt
    33361 attttaaatt ttccatcaat tacattacac tttccatagc taatgctgga tgaccttttt
    33421 cttctaagaa agctaatact ccttcagaat cgtcagctat agtttcatca caaaccatat
    33481 cacagaagtt ttctattcca gtcatttctt taactgtagc atttaattta tcagctacat
    33541 aatcttttaa ttcttttggc atccaaacta ttctttctgg tccaccttct gcataagcga
    33601 attttttaga agatataaaa tgtcttccgt gtcccataaa tcctggagtt tgaactcctc
    33661 cacctgtcat agaagcaagt tctccgaaag tcataccaac tggagtaaca cttgcaaatt
    33721 ctctgttaac tacaacaaat ccatttgctt ctggcattat accacagata cattcgaagc
    33781 atccacaaga agtcattggg tcttctagta tagagtataa tgtaacagat tcaactgcac
    33841 cttgagatat ttggtttaca gtttcattta ctgaattcca tacacctgtt ctatcatcta
    33901 agcactcgcc tttttctatt ggttgacaag gacctgttgg gtctaattct ttagtagcct
    33961 tagcatctaa ccagctaact gctccacaaa gaccaagtct ttcaggagta acaacacaaa
    34021 cgtgagctgg tgcaaatgat tgacataagt tacaagaata gaaagtatca acactttcat
    34081 caactaatga agctaatctt tcatctctag cattgtactt agctattgct tctcctttta
    34141 attcagaaac tttttcagca tctgtaatta tagttatttc acacttatca actactgatt
    34201 caaattcatc taacatctta gcatataaaa cttctcctat atgctcaagt ctaaatcctt
    34261 tatcaaatgc atctttagag attcttaccc aagccatatc tctttgtcca acgtgcatta
    34321 caccttctat atagtttaag aagtagtgga aacgtctttc aagaactggc tcgaaatctt
    34381 cttgcatatt tttaccagct attttaacta ttacagcaag tggtaatctt aatacaccat
    34441 ctgcatcaac ttcatctata tttggcccta ttatttctat cttatggtct tcaacttctg
    34501 ttgcttcttt cttaacaaca agctcccaag cttcagttct gtttccacca aactcagcaa
    34561 acatatcgct ctttctaatt ctctcacctt caaatgctgc tgcaaatgaa actgggatag
    34621 gtatttcagt aacttttatt tttattcctc tagcttctaa tgaagtagct actatcttat
    34681 cgtaatcttt ttgagttaat aggttcattg gaacttcaag aactgtttgg tcagttataa
    34741 ctgggaatcc taaagctatt gctccagcac ctgcagatac aactaattca cttaatggtc
    34801 caaatgcatt aacaaatgca ggaactctat tagctgtgta ctctaataat ccgtttaaat
    34861 caccaggagt taatccaccg aatattaatg ctgctcttac tgcaacagat acaacgtgta
    34921 taacagaagt tacatcatat cctagaggta taactcttag ctcaagaccc atttttactc
    34981 cagcttctat agcttggtct ataactttac caactaagaa agttagtaaa cctttagatt
    35041 ggtaatcttt tataactttt gctgctgatt ctgcatctgg acactcacca agaacaactg
    35101 caacaccagg tatatctcct gtaactaatg gtacaccaag tgatctgatt atagggtcag
    35161 ttatatgacc tgcacatggc tcactatatg gagcatccat agttgaatat tttaatgctt
    35221 caattacttc tgctgctaaa gctgtagcta aaccagcatt aaaagcatgt tctagtaaat
    35281 gagttctatt tattaaagat tttactactt ctaaagcacc ttctaagtcc cctaaagtgt
    35341 tcatcttttg tcctgttgca gcatatatac aaggtaatga atatgctgta tcagggaaag
    35401 caactttatg ctcttttcca ttcttttcta tagcttcagc taacatagct tgagccgcac
    35461 ctaaagcttg ttctgaccct gtaaagatta tattatatag attcattcta tttccccctt
    35521 taatatttac acctattttt tattaactat accctaaaaa ccctagaaag caaatccacc
    35581 ttcatttaac tctaagaaag agtctgcata taaagatgcc ccgttgatta tgtcacaaga
    35641 ttttacagtt ttcattaatt ctgggtcaca tggattcatt atagctgaac taaatccatt
    35701 agccattgcc attgctaaga atgcactatc taaaacaggt ctaacatgct taggacatcc
    35761 attagatacg ttagataatc ctcctgtagt taaaagtccc atctcagtca tcatttttat
    35821 agcttctaaa acttctactt gtttttcttg catacctttt ataactaagc ataatgggtc
    35881 aaatagtata tcagttggct ccattcctaa cattaagcct ttttctaata tttcttggca
    35941 gtaagccata cgctcatcat tatctcttgg gataccttct tttgcacata aacctataac
    36001 cattgcttcg tattcagctg ctatatctat taaatcaaat cttgagccag catcagcaga
    36061 gtttattatt ggttttgcat tagttctatc ataaacttta agtccagctt cgatagcttt
    36121 tttattagct gtatctaatg ctataggaac attgttgaac tcagattgaa gtaatttgat
    36181 tccccatgtc attatttctt cgccatctct ttcggcaggt cctatattaa aatctatata
    36241 gtgtgctcct gcttctaatt gctcttttgc tctttttaag attggagctg ggtctctttc
    36301 tgctaatgct tttcttattg aaggtgatat acaatgtatt ctctcaccta taatcataaa
    36361 tttttccatt tataatgtcc ccctttaaat ttaatattaa tagttagtta atattttatt
    36421 agtttgtttg gcatgctttt tcttggtatc cttttaagaa cttaggtaat tccatagatt
    36481 cctctggtcc tataactaca ttccatcctg gtaagttatc ttctatatca cctttaagta
    36541 ctgcaacttt tcctggaatg ataaggtctt tacaattagt tacttcttct accttacttt
    36601 ctttaatgaa agcagagatt gagtttccac caaatttacc agcagcccaa gatgtaagaa
    36661 ctgaatatcc acctgcatct ggtattacta accatactgg aacttttgat ctttctatat
    36721 caccagcaac tatgaagtaa gttaatgcaa agtcaacagt aactaatact ggagaatttt
    36781 catctggatt gttgattgga taaatctttg gttcaactct cattggtctt tgtgggtcag
    36841 tgtatatgtt ttgtcttagt gcaaataatg gtaatgcctt agcataacta atatcatcta
    36901 ttactattat agaaccgtat tttatagtga atattgatga taaagcaact tccatcatag
    36961 ggtttgaatt agataatcta tttgcaaata ctattgaagg atatccaaat gttctatctt
    37021 gctcttttaa agctgttctt ctaacttgta tagcattagt gtaagtatct tttatgtttt
    37081 ctcctgttac atctaatact aattctttat atccagcggc ttgaattaat tcaactgttt
    37141 catataattc ttctaagcta ccagctttta cacctaatgg taaacttgct cctttaacta
    37201 cttctatcat atctttgtag ttctctttag ttgctccata taccattggt ctagcatctt
    37261 ttaatacttc aacagcttct ttaactactt gtgcatcatc acaagctaaa atataagcta
    37321 cttctaatcc actgcctttt attttatcta ttaattttaa atacgcatct ttatctccac
    37381 aatactctaa aacagccatt tcaactttca tttgttcacc aattcttaca tagtcaactt
    37441 ttttcatgtt agctatttta gcatctactg cttcatcact catacaagta cagaatgaaa
    37501 ctgcatatct atttctactt actaatgttt tttcatgtct aaataatact gtttctccac
    37561 ctagttcata ctcagatgca ccagcaccaa cttttaaagc tttcattaaa ggtgcagtag
    37621 cctctgataa ttttgctata gcgtcatcag acatatgtgg gcatttacct acttctacag
    37681 ctcctgaagc aaccttcata gagaaagcca tacaagttgg gaatccacaa tccttacagt
    37741 ttttctttgg tgttaattta aatatatcta aagcttttaa tgccattatt ttttccccct
    37801 aaccttaaca atttaattaa gctaatgcgt taaccaattc ttttatagtt tctattgatt
    37861 ttggatgacg aagtataact gcgtttgaac cacctactaa aacacttgct gcagttgaaa
    37921 cttccattgc tatagttctt tcttctggac aaccccaatc tggctcatct tctattggag
    37981 ctatagcttc tttaacatga cctacttcaa aagctactgg tgttatgata ggcatttgta
    38041 atgtcttatc attttgacca aatgctgcaa gtctaattct atccatagta gatgcaacgt
    38101 actcatatcc ataaccaact gctgaacatc caacattcat aactatattt tcacctttaa
    38161 cgcctagttg agttaacaat acgtttaatt gtttagctag gtttatatca acagaagatt
    38221 cagctcctac tttatgtgca taagccatac cagctgatgc tcctactcct ttgtagttat
    38281 cttctactgc tgacataaat aagcagttat gtccatccaa tgtttgagct aatttttcaa
    38341 ataatttacc atctttttca tgatttcctg aaccagctac aactaaaggt aattttattg
    38401 cttcaattac tgccttagct acatcagcac attcatcaac tgacttatct aatccatttg
    38461 ggtcagaacc atcaaatttt aaacaaataa aatctgcttg tgtattagct tcaacatatt
    38521 tagcccactc tactggacaa ttagctacat ctttgtataa ttccttatat gaatcagtcc
    38581 agctttcagg ataaacgtct gatatttgta tacctatttt tggagaatta cctacttctc
    38641 catcaaaact ataaaaaggt aatacatttt ccccacctaa tttaattgct ttttccccta
    38701 ttcctacttc aacttctgat atttttccag aatatttttg agtagacatt ttaaatgcca
    38761 tatttttttc ccccttaagc ctattttaat tctaatttag caaaaatttc tttcatagcc
    38821 actttagatt ttgaatcttc aggtaaatta actaaaggaa tacctgtaga atcatattca
    38881 tatatcaatt catccatagg tacaacaccg attagctcta ggttatgctt ttcaatttct
    38941 tctttaacac catcattcat aaccccattt ggaactttat ttacaatcaa aagtattctt
    39001 ccaacactta actttagttc ctcagctaaa tctcttattc tagcaacagc ttgtatgcta
    39061 cgtcttgagc aatcactaac caataaaagt gtatcaacat gtctagtgac ttttctactt
    39121 agatgttcca taccagcttc gttgtccatg actaagtatt tgtaatgacc agatattttg
    39181 tttacttgtt ctctaagtat cccatttaca aaacagtaac atccttcacc ttctgaccta
    39241 cccattacta ataagtcata tccttcgcct tcttcgatta tagaatttaa tctaaattga
    39301 agatattgtg cctttgtcat accaccaggg aatgcattgc ctaacttttc tctttgattt
    39361 acttcttctc ttatttctcc tattgttgct tcaacttcga tacctaacac ttcatttatg
    39421 ttagcattgg catcagcatc tacaactaat actggtccct ttttgtcctt aaccaaataa
    39481 tcaatcaaaa gccctgtgag acttgtttta ccagttccac cttttcctgc aacagctata
    39541 ttgtatccca ttttaagact tcccccttaa atctagccac agagccttat gtactctgtc
    39601 tgtctattat aatttaggta cagaatgaac acattctcca tgtacatcat gaagtgcttc
    39661 cattagccct tcagataaac ttggatgtgg atgtattgca tcccctactt gttctactgt
    39721 taatcctaga tgaacagcaa gtgacaactc tgtcaataaa tctgtagcat gaggtcctac
    39781 tactgctgca cctattatct tatcagtttc tttgtcagct ataactttta caaatccttg
    39841 gaaatgtcct atagcttgtg cttttccaag acctctaaaa tcaaattgac ctacattgta
    39901 ttctacacct tcagcttcaa gttgcttttc agtcttacca acaccagcaa cttcaggttc
    39961 agtataaaca catcttggaa ttgctctata atctactacc ttagtcttac ctaaagcatt
    40021 ttcaactgct acgatacctt ctttagaagc aacatgtgct aggaaaggag tatctattat
    40081 atcgcctatt gcgtatatac cttctacatt agtttcaaga tgttcattaa ctactacttt
    40141 tcctctttcc atttcaattc ctatatcttc tactccagaa ttgtcaaggt taggtcttct
    40201 accaacacat actaatgcat attgtgcttc tataaccttt ccattagaaa gagttgcaac
    40261 agccttacca tcaactactt cacaagtttg tactccaata cctgtaataa ccttaatttt
    40321 atctttctta aattgtctaa gtagttgttt agctacatct ttgtcttcat ttaatagtat
    40381 ttggtcaacc atctcaacta tagttacttc tgttcctaag gctctaaaga actgccctat
    40441 ctcacatcct ataactccac cacctactat taacatagac tcaggtatct cttcaagtcc
    40501 aagaacttca tcactagtta ttacaacttt tccatcatat gggaacattc ttggtacaac
    40561 tggaacagaa ccatttgcta gtattatctt atctgctttt atagtttcta ctgttccatc
    40621 atctttagtt acttctatag tgtttttatc aattaatttt ccaaaaccat taactaagtt
    40681 tactcctctt ttttcaaata agaactctat accacttatt aattggttta caactttatt
    40741 ttttctttcc attatggcag tgaagtttgg cttaattgta ccatctattt ctataccaaa
    40801 atcctttgct tcttttacag tattcaatac tcctgaagaa gcaagaagtg ctttagttgg
    40861 tatacatcct gcatttaagc aagttcctcc aactcttctt ttttcaacaa ctgttacatc
    40921 tgcaccaagc atagaagctt ttatagctgc tacatatcct cctggtccac cacctactac
    40981 tactatcttc atctttaatt cccccaaacc taatataatc tacaatcttg caatactata
    41041 aacctgcggc atccagtata gcaggtatat tctcttctgc cccaattgcc attatatgaa
    41101 caccatcaca taaatcttct tcttttaatt gtttgataag ttcccctgcc atttttattc
    41161 cttcacttac ccagttttct tttccagctg ctcttaatct ttcgatttgt tcatctggaa
    41221 caaatattcc tggtacattt gcagtcataa atttagccat tcctggtgat tttaaaggta
    41281 ctattcctgc taataccttg caatccatat cccttgttaa ttctctaaac tttctcattg
    41341 tatttatgtc ataaagtgct tgtgtttgga aaaatttagc ccctgctttt attttctttt
    41401 tcatttttaa taattgaact tctattggag aatattctgg agttactgat gctcctagat
    41461 aaaaatctgg agaacctttt aacttattac cagccatgtc cgtacctgcc attaaagtct
    41521 cagcagtttg aagtatcccc acagaatcta aatcaaacac tcccttagct tgtggatggt
    41581 ctcctacgct agtatggtct cctgttagtg ccaacagatt gtttatccca aatacacctg
    41641 ctgacaacat ttctccttgt atagctattc tatttctatc tctccctgtt atttgaataa
    41701 ctggctctaa tccagcatct tttaataatt tacatgtagc aagagaagtt gctctcataa
    41761 ctgccgattg aaagtctgtt acatttgctg catgaactct tccaactaac ggctttgcac
    41821 attctattaa atgtgaaagg tcagtccctt ttggaggagc catttcagta gtaactgcaa
    41881 attttccact ttctaatgtt tctcttaata agctcatctc taatccccct tttaaccttc
    41941 taaaaaaact tttatgaatt tgctgctgct tctttctttt tctttgcact taaacttctt
    42001 ggattatttt gcttagaata gtcttttggt ggtcttattt ctgctaagtt atctaattgt
    42061 cctatagctt ctagtctttc atatatcttt atccaagcac aatcattctc tgagtttact
    42121 tcacacttac catttttagc tccaccacaa gccccattca taagaccttt agcacacata
    42181 gttactggac atatcccacc tgtccaacca agttcacagt ctccacaagc cttacaggct
    42241 tcttcatatt ctccaactct ttgtacttcc cctataaaca aagtattgtt ggctggataa
    42301 actggcttat cttttaaatt cttaactatt gtctgtgtac catctccaca tgctaaagat
    42361 aaaactgcat cagcttcctt agtctcttct tttagagcct ttaaatcctt tttagacttt
    42421 aaaaggttac aagcagggtc aagcattaca tatcctaaga tttttttgcc ttcaccttca
    42481 agagtttcct tcatttttaa aacttcttct tccccaccac ttttacaagt tgctgcacat
    42541 tgattacaac caactaaaac cagcttgtca aaatctttta agtaccctag cacttcttct
    42601 aatggtttat tttcagaaat tatcatatct acttcccccc taaaccttcc acatatatat
    42661 atctatttat tgttttgtaa tttgcaagcc ttaactacat gttttgcaag tatagatgtt
    42721 gtaactgaac caacccccgc tggaactgga gttatcattg atactttgtc taaaactgca
    42781 tttgtatcaa catctccaca taaatttcct tcttcatcaa catttatacc aacgtctata
    42841 actaccgctc catctttaac aaaactttca tcaaccatct tagctctacc aatagctgct
    42901 attagcacgt ctgcctctgc tgctacacct gatagatttt tagtttttga atgacatatt
    42961 gtaacagttg cgtgttcact taataaaagc attgatactg gctttccaac taccattgat
    43021 cttccaagaa ctgttacctt acttcctttt aaatcaacat tatagtgttt taatatctca
    43081 actactgctg taggtgtaca tggtggaaat ccacttttat ccccttccat taccttagca
    43141 gagtttattg gactaaaaca atctacgtct ttttccggtg caataacata ttttataact
    43201 tcttcattta agtgttttgg aagaggtcta aaacataata taccattaac atttttgtca
    43261 tcattaactc tctttaatac atcaatatat tcctcttgtg ttatatcctc agctaactct
    43321 aacacttctg ttgttatacc tatgttttga catctcttta gtgctcctct ttcataagat
    43381 aaatcatcac ttcttgcccc aactctaact attgttagtt taggatttat accttcttta
    43441 attagtaaat cgacttcttt tattaattct tcactaatct tatcagctac aggttttcct
    43501 tttataattt gtcctttagt tgacattcct tccatacatt cacaactccc ctacaagcaa
    43561 acttaaatta atttattcta tataccacat attaataaca tagtatctac tttcgtcatt
    43621 taaaaattat tttccaagag ctttttcaac tttggcataa acttcatcac aaatttttac
    43681 accatcttca actaacttat ctacttctgt ttttacctta ttaacatatt ctttatcttg
    43741 tatagaattt atatttataa ctacatttaa ttgtccacca agtatagcag ctcttaaaca
    43801 ctgaactcct acaccaacat cacttatagc aagtcttgaa cctttatcaa ctaaatcttc
    43861 atgtaactta atagcttcat agcaaactct tactatgttt aatggtactt cacaagcaac
    43921 ttttaatgcc ttttccattg tttctgcttt tattcttttt tcttcttctg tttctttagg
    43981 aagcccgtaa gcttttgata atggtaagaa acattctgca tcatcatcta tcatttttaa
    44041 taaatctttt tctaactttc ctgccttact taatatctct tttacacttt cttcatactc
    44101 agcatacttc ttcttcccta ttgtaaggtt acataccata ctacctaaag ccatacctat
    44161 tgcacctact agtgcagcag ctcccccacc acctggtact gcttctttgg aagatagtac
    44221 ttctacaaaa tcaacacaag ttttatctgc tattttcatg ataatcctcc ttgaaaggat
    44281 gtaggaaaat atctcaaatt gttcgagata ttttacgaac tattttaaga tatctcctac
    44341 tctttattta taaattaatt tccttagaat aatccagcta ttactccatt ttcatcaaca
    44401 tctatttttt ctgctgctgg aacttttggt aatcctggca tcttcattat ttctccagta
    44461 agtgcaacta taaatccagc tcctgctgaa attgttgctt gtcttacagt tattctaaat
    44521 cctgttggtc tacctaattt agtttggtca tcagttaaag aatattgagt cttagccata
    44581 catactggaa ctttagtaaa tccaagtttt tctaagttag ctatttcctt atcagcttca
    44641 ggagtataat ctacaccatc tgctccataa atcttagtag ctatagcatt tatcttgtct
    44701 tttatagata aatcatcttc atagcagaat ctaaagttat tttcttcttc atccaataat
    44761 cttaaaactt ctttagctac tgcaattcca ccttctccac cttttgccca tacttcagaa
    44821 agagcaacat taacacctaa ttccttacac ttgtcttcaa caagtttaag ttcagcttca
    44881 gtatcaagtg ggaatctgtt gattgcaaca actgctggta atccatatac ttgagttatg
    44941 ttttcaacat gttttaataa gtttggaagt cctttttcaa gagcttctag gttttcgtta
    45001 tttaagtctg ctttagcaac tccaccattg tattttaatg ctcttacagt agcaactatt
    45061 ataacagcat ctggttttaa gtttgccatt ctacatttta tatctaagaa tttttctgct
    45121 ccaaggtcag caccgaaacc agcctcagtt attacataat ctgcaaagtg catagccatt
    45181 cttgtagcta taacagagtt acatccatga gctatatttg cgaatggtcc accgtgtaca
    45241 aatgatggtg ttccttcaag agtttgaact aggtttggtt ttaaagcatc ttttaataat
    45301 gcagccatag caccattagc ttttaattgt ctagctgtta caggttcacc ttcgtagctg
    45361 tatccaacta ttatgtttcc taatctttct tttaaatcag atatatcact tgccaaacag
    45421 aacgctgcca ttatttcaga agcaacagtt atatcgaatc catcttgtct aactgctcca
    45481 tcaccttttt tacccatacc atcaacgata tttctaagtt gtctatcgtt catgtctaca
    45541 catcttctcc aagttatttt cttagggtct attctaagtg catttccttg gtgaatatgg
    45601 ttatcaagca tagcagctaa aagattgtta gcagctccta tagcgtgaaa atctcctgtg
    45661 aagtgtaagt ttatatcttc cataggaaca acttgtgcat atcctccacc agctgctcca
    45721 cctttaacac caaatactgg tcctagagat ggttctctta aagcaactaa tacatttttg
    45781 tctagtttag caaatgcatc agcaacacct atagtagtag tagtttttcc ttcacctgct
    45841 ggagttgggt ttatagctgt agttagtatt aacttagctt ttttaccagt ctctcttttt
    45901 agtagattgt agtcaacttt tgctttgtat ttaccataaa gttctacatc atcttcacct
    45961 agtcctaatt tttttgcaac ctctctaata tcttgaggct tagcttcttg agctatttca
    46021 atgtcagatt taaatcccat aactgaatct cctcccacaa cctaaataga tttaaaatat
    46081 aatttttgta aaaattgtat taatatgtaa aaattatatt ttataagata attatacatg
    46141 taaaattact taaaaactag ctactaagct attaaaatat tcaaacttaa tagctagtta
    46201 tttatctaat tacttactta ttacctatct attgcctatg tctaaccttt aaccaactta
    46261 aaatcccaaa tcaaaatttc tatctatttt atttatcctt aagtgacatt aatttatctt
    46321 ttattgcttt aatctgttct ttcgttgttt cactagaatc atatggagat tgattagctc
    46381 tatcagaatc aataacatct tggttataat aaataaatcc taaagattct ccatcttcta
    46441 agttttggat tataaattct tcatcttcct tatttctaat tttatttcct actacaaata
    46501 ctttatttac acctatatca tgtccaagtt tcttaacttt tctataagtc tgtagacttc
    46561 tctctcctgg ttctacaact actataaaag catctacacc ttgggctgtt cctcttccta
    46621 agtgttcaat tcccgcttcc atatccataa ccactacatc tttattttgt agtattaaat
    46681 gtgagcagag tctcttcaaa agaacatgct ctggacatac acatccagta ccaccagaat
    46741 ctacggttcc caatgtaagt aatcttactc cattgtattc tttacagaaa ttttcaggga
    46801 tatcgtctac tttaggattc atcttaaaca tttttccaaa tgaacctaca gaagcagctg
    46861 ttctatctga aactaatttt ttcatttctg atataggtac tattgattca tatacttctt
    46921 ttggaaaacc tagtgctaaa gctaaattag catcagggtc ggcatcaaca gcaacaactc
    46981 tgtatccgtc ttcagcaaac atcctagaga gcattgaaga aaaagtcgtt ttacctactc
    47041 cacctttacc tgttattgct atcttcataa tccctaccac cttttattac tatttgcttt
    47101 ttagtagtta attttacagt taaatatttt ttatactcta tatttttata cactaaaaat
    47161 aaactacagc tagtccttac aggaaccaat taaatttacc ctactagctt ttcaaagcag
    47221 catgtatagt tttattattc tttaatattg tttatatttt atttattcag tatattcatt
    47281 atattttgat gttctttact ttaatatttg actttattca actttattca accagaaact
    47341 tcttataaaa acgtttattc tataacttca ctatttttta agcttcagcc ttttctgcca
    47401 tcttttcttc aactaatgct tcaaagtgaa cacgtttttt ctctatatct tctattattg
    47461 tagctgctaa ctcttttgga tttgtattta cagtaaatgt agctccaact ttatctttta
    47521 attcactagt taatatatcc actgctcttg aagaaccagc tattggaggc attattccta
    47581 aataagtgtc tataccagaa gctacaacat agcatcctat agcaactgct ttttctgaca
    47641 tccactctgg agctatacct acaactggaa ggtcacacat atccatatct aagtaattag
    47701 ctgctgctcc aactaaatct agtatacgac ttatatcaac acaagaaccc atatgtagta
    47761 ctggtgggat tcctactaat ttacagacag ttgcaagacc tttacctgca tatttttcag
    47821 ccgcatcaga ttccattaaa ccaaacttag ctgctgctga agctccacaa cctgtagtaa
    47881 ctactattat gtcgtttttg attaattctt tcattattgt tacgtgagcc tcatttgaaa
    47941 ctcctttagc attgttacat ccaactacac cagctgctcc tcttaatact ccagatttta
    48001 aacagtctgt taatggctta actgttcctg ctgggtctat atgagagttt acaactctgt
    48061 ctaattgatt tactactgct tctacactat acccaactgt tgcgcctgat tttaattcag
    48121 gtataagtac ttttgattta tctctatttt taaagtttaa aatagcttct ttaactattg
    48181 ctttagcatc atcaagtgct tgttcttctc taaattcaat ataagttgaa cctgttatct
    48241 tagcttttgg agaagttgtt acaaatttag tatggtaaca atctgcaact ctagctaatg
    48301 cagggaatat acattgaacg tcaactatta cagcttcaac tgctccagtt attattgcaa
    48361 gttcttgttg atggaagtct cctgctattt ttacaccatg tctcattgta atctcattac
    48421 ctgtacaaca catacctgct aagtttattc catcagcacc aacttcttta gctaaagcta
    48481 ctaagtctgg ctcttctgat gctaaaacta tcatttctga taatgctggc tcatgtccat
    48541 gtaatattat gttaacttta ttttcttcta atactgctaa attcgcctct gttcttcttg
    48601 gcactggagt tccaaataag atatcgctta gacgagtacc tatcatagaa ccaccccaac
    48661 catcagccat agaagttcta agactcatgt gtattaaact atctatatca ccagtacatc
    48721 ctatatgagt agagtgcatt attgttgcaa tttctctatc tatagctctt ggctctatag
    48781 cgtattcttt ccatattttt tgtcttggtt ctggtgcatt ttttataaat cttaatgtcc
    48841 caaaaggttt tccaaattcc attaatgcaa tttctgccac ttcatgtgct acatcataga
    48901 tatctctacc ttctgtctct acatcccatt ctttagctaa agtaattaac ttagcttcgt
    48961 ctcttatttt atagtttcca tttggatcag ctaatgctaa tgtatgtgct atatctctag
    49021 catggtctga atgggcagct gtcccaccag caaccattct tgcaaagttt ctacctacta
    49081 tagtatgctc atctgctcca cataaccccc ttggagtctt agggcttatt ctacatggtc
    49141 ccattccaca tattctacag caaacacctt gaagaccaaa accacattgt gccttaaagc
    49201 cttcttttct atcatacatt gtttctacgc cttcttctct agctttagct atcatcttct
    49261 ggctgtttaa atcaattgtt aacatttttt catccatatt cctatcccct ttttttgatt
    49321 tttaaaatcc cccaggtgac tgacttcatg tcataaaata ataaaataaa tgctttctaa
    49381 gttgattata tactaatttt cagtgctttg ctttagattt aaatattttt ttaacaaaat
    49441 tagataatac ataaaaattt tattttctac acttaattta agttgatttt atcgcttgtt
    49501 tttggctatt tgttaatatc taaacctact tttttgatac ttcagtttaa ttaaattatg
    49561 ttttgatttt tttaaatcaa atttgaaatt tttctagtca tttttatatt attgcagatt
    49621 gtttgtgtat ttattatctt tcactactgt ttggacttat tacagatttt tactccctaa
    49681 acttaattag taaaaacttc ttagaatagc attttttgga ctaatttttt tgaatttttt
    49741 tatttttcct actttaatat taattatagc ttcaaattga acatttgtat atattcattt
    49801 gaagctataa ttatttttta tataaaactt ttttaataca gcattaattt agacattttg
    49861 actatttttt ctactttaat tcatttattt caagcgattt atcctatttc atatttttag
    49921 ctacaatagc cccatatgca atagaatata atttagattc atgtgaatca gctcttgaca
    49981 ctaaaactat aggagctttt gcacccataa ttatacctgc tgatttagag tctgccatat
    50041 atgtaaaggc tttccctatt ccattaccaa cctctattgt tggaaccaat atgatatcta
    50101 catctccaga tacttcactt ttaaaccctt ttatctcact agcttctttt gaaacagcta
    50161 aatcaaaagc cagaggacct tctacaatta cattttcacc aaattctcct tctttacaag
    50221 cttttgctaa catatctgca tctacagttg cttgcatctt aggatttact ttctctttag
    50281 ctgccaaaca tgcaactttt acagtttcca ttcctaaagc ttttgcagct tttatagaat
    50341 tttttaatat ctttgccttt tgttcataat caggagctat attcatacct ccatctgtag
    50401 ttataagtaa tttatggtat ttttctaact catataccat aacatgactt aataaactat
    50461 cagttctaag accatagtct ttatttaaca cttcttttaa tatgacagat gtatctaata
    50521 atcctttcat tacaaagtcg gcttctttac ttgatactaa tttaactgct atttctgcac
    50581 actctttgat atcatcctta tccactattt tgataccact taaatcaaat cctatttctt
    50641 ttgatatttc tcttattttg ccttcatttc caaccaatat aggagtaatt atatttcttt
    50701 ctacagcatc ctttattgcc attaaaactt ctttatcatg agctgctgct acagagagta
    50761 caattttctc tcctccctta agttgttcta aaatatcatc taattttttt atcaaaagta
    50821 acccctccct tatatatatt taactaaata ataccatatt tgctatacct tttctcaatt
    50881 tattttttat gttataaaga atttatataa ctaaaagaca gtttaatatt ttattctggg
    50941 atttcatcaa taactcttaa aagctttgca ggaacaccac ctacaatagt attttctgca
    51001 acattttttg ttacaactgc tccagcagcg ataattgcat tatctccaat agtaactcct
    51061 ggaactacag ttacattagc accaatccat acattttttc ctattgtgat tggagaagga
    51121 taagttgtac cacgtttctc tggtgcaata ccatggttaa gtgtagccaa taccacatta
    51181 tgcccaattt gcgtattatc tccaatctca atccccccct ggtcttgaaa atggcaactg
    51241 ctatttataa aaacattttt tcctattgtg atgttttttc cacaatctgt ataaaatggt
    51301 ggaaacataa aaaatgattc atctaccttt tttccaatta actcagaaaa taaactacgg
    51361 acttcttctg gttcatgata agaagaattt aacttacttg tgattttcat tgcttcatta
    51421 ctcaacttaa ccataaattc atgtatttct gatttactag aaacaggttt tctttgattg
    51481 atatgtgtta aaaattcatt gatattcatt ttatacatcc tttcaatatg tgctttaatt
    51541 tatttgtaca ttctaaataa tattttaata aaaattaatt taatttattt ttattaaaat
    51601 atcattgctt cttttagtgt aatatacaat tatatctata tcaaatatct atagtaaata
    51661 cttaactata cttaaaaagc atataaattg atataattat ataatagata agttaatatt
    51721 gaggagggat aattatggaa ataagagtat taagatattt tttagcaatt gcaagagaag
    51781 aaagtatatc tggtgcggca acctttttac atataacaca accaacactt tctagacaaa
    51841 ttatggaact tgaagaagag ttgggagtta aattattggt tcgaggaaat agaaaaatca
    51901 ccttaacaaa tgaaggtagg cttttacata aaagagcaga agaaattata tcattagttg
    51961 ataaggttga attagaattt gacaatccca atgagattat tagtggtgat atatatatag
    52021 gaagtggtga aactgaagct atgcgactta tagctagaac tgcccatcat tttcatcaaa
    52081 aataccccaa tgtaagatat caccttttta gtgggaatgc tgatgatgtg actgaaaaat
    52141 tagacaaggg actgcttgat tttggtattt taattgaacc tgcaaatata cagaagtatg
    52201 attatgcaca aattcctgct actgatgtat ggggtttact aatgcgtaaa gatagtgccc
    52261 ttgcatcaca aagcacaata aatccaaaag atttatgcaa tatacctctt attatttcaa
    52321 gacagacact tgttgataaa gcaatttcta aatggcttca agacagtaat gaaaaactaa
    52381 acatcattgc aacatacaat ttagtttata acgcttctct tttggtagaa gaaggtctgg
    52441 gctacgctct gtgtttagat agactcgtca atactacaga aaacagtaat ctttgtttta
    52501 gacctctatc tcctccttta gaagctaaat taaatattat atggaaaaaa catcaaatat
    52561 tttcaaaggc agcagaaaaa tttttacttc aaatacaaaa agatttttct tctatcatgt
    52621 tggatacatc taagttggat acacctaaag aaaaataaat cataataaaa ggtgagagtg
    52681 tctcaaaatg aactttgcac tgtataaggc actctttttt atacaaagta aaatatatgt
    52741 tcatcatttc agcactaaaa aagaggctta tctctatttt gagatagccc cacttaacca
    52801 ataaaattaa atacaactta acaatatcct tacttattta ttctaagttc tcttagacaa
    52861 gcaatagtaa tttcatcttg tggatttaat tcataagctc tttctatata gtaagttgca
    52921 tcatcaatat caccattatt taaataagcc attcctaaat tgcaaagtat ttctgggtct
    52981 tcctttatct tagccgcttg ttcaaaatac tctattgctt tatctaaatt attcttataa
    53041 gcctcacaaa gccccaattc cactatagta tccacttgat taggctttat tatcaatatc
    53101 ttctcaagat acatcttagc ctgctctatt tcgcccatac ccttatatcc aagtgctatc
    53161 ataaacagca agttccacca atccatatgt tcttcttcta atggaagtaa cttctctaat
    53221 ccttccttaa acttaccttg gaatacaagt gtatatcctt cttcatattg aactttaaag
    53281 tccatcttgc ccagattctc ttgtacctca gctactaaat cagcatctaa ccctagcctt
    53341 aacgcttctt cccaagtcag cttagtcttt aaatattgac cttgattata ataatggtag
    53401 cctaagtgat aataacctaa tgcaaaattc tcatcaacat caattacctt ttcaagctta
    53461 gccatggctt caagtaagaa tgcattcatt gccttatcat ccatatcttt ttgatactgt
    53521 ttagctatct cttggcaaac aatcgcatag ttatacattg catctaaatc atctggatac
    53581 attgttataa atgcttttat atatattaaa gaatctgtat gttcaccaat ctcaaagtat
    53641 tttctcgaca tatatcccat atacgatttc aaatccaaat caatatcttt tgataaagca
    53701 tttaaaaact tctcatactc tgcattgttt ttaaacttac tatctattcc aattatatat
    53761 atcattgcat ctgctataga cattgaattt aacttatctt gagcagtctt ttcttttatc
    53821 ccttttacta atacctcatt ttttataggt acatctaatc caccttttgg cacttcatag
    53881 ctttttaatt taaactctcc accatgtttt atagttataa aagctaattc ttctgctttt
    53941 tttagcaaat atttttctat cttaaaatcc attttatcct cctaaatact taaatctcta
    54001 ctttatgagt tttagatttc tcaaatattt ttttatcatc atactcaaat aagactacat
    54061 tttctacttc tcttatttct gtatttatat cattttttaa atccacaaca ttaaatctaa
    54121 atggctcaaa atgaacatat tttattatat gtttagctgg tttatcaaca aaactaggta
    54181 aatatgttga taagttatca ttatcttgta aaaatacatg gcatctattc ttaaaatcat
    54241 ccacatctag cttattaaaa aactgtggta tatttgatgt tttacccagt gatatatagt
    54301 catattttaa tatatcgttg aatacatcat tatctatgtt gattttttca ttatagaaat
    54361 ccatcaatat tttatataac tgattctttc cttgtgccaa atcaaagtag ccattttcat
    54421 caaaatactt ggcaaaaacc tcaaagaatt taaaagggct ttttttataa aatctgccta
    54481 taatatatct catagataac acaaagtttt tagaattgta gtatctctcc agtatatctt
    54541 ctatatcctt tagcttcaaa gtttcttcat agcttatata attattgtat aaaaactcat
    54601 atggagcata atccttatat ttaaaaccat gctcatctgc aacttttctc atacctgtcc
    54661 ctttaatcat ctttaaaaag cctaattgca aatgctctat acctagatta aatacatcat
    54721 taaaagaatt ttcaaagctc ttataatctt cataaggcag tcctgcaatt aagtctaagt
    54781 gctgatgtat atttctataa gatgctacag tttgtacaac atgagacaac tttttaaaat
    54841 catcccttct acctactgca tcaagtgttt tttcattcgt tgactgaaca cctatttcaa
    54901 attgaaaaag accttcttta caatcagcca aaaactcaag catcttatca tctatcaaat
    54961 acgctgtaac ttcaaaatgg taagttgtat atccattatc atttttcata aggaaatcca
    55021 tgatttccat agccactttt ctatttgcat tgaatgtcct atctataaac tttatctgtg
    55081 atactcttgc atctattaaa gcttttaaat cactctttat cctgtctaaa ctaaaatacc
    55141 ttagacctgg aatagtagat gataaacagt attggcagtt aaaagggcaa cctcttgatg
    55201 attcataata cactattcta ttttcatatt cttttggatt caagttttca taaggactag
    55261 gaatctcatc caaatcttga agtaattcca taggcttatt cactattatc tcattgcctt
    55321 gtctatatac aagtccattt acatcattta tttccatctt tccttgcaag tgaagtacta
    55381 agtctctaaa aattaattct ccctctccat aaagtatata atccacaaaa tcatactttt
    55441 tcattgcctc ataactatca taactcactt ccggtccgcc caaagctatt ttagtattag
    55501 gtctaatttt ttttagatta tcacaaagtt taactatatc tcccacattc cagatatatg
    55561 ttgagaataa tattatatca tattcatttt tatatatatc ctttaaaata tagtccaaat
    55621 cgttatttat cgtgtattct ttcatattaa cttctatcaa atctcttaca aattctttta
    55681 gatatcttat agccaaattt gtatgaataa acttggaatt taatgttgtt aataaaattt
    55741 tcaaatattt ctcacctctt tttaagatta tactgtcttt ttgtacccct tataggttaa
    55801 aatagtatat gtaaagtcta aataatatct atataattaa tttattaatt tcaagtaatg
    55861 tgaggaaagg aagtttttta tggaattaat tgaaaaagta tctatcaact ctttatcaaa
    55921 aagagattta ctacttatta taaagtctct tgaatacact aatgaaaata ctcaaattag
    55981 tgatttcatt gaccttagga ataatatagt aaaggaatta tgttttttaa ctgacactaa
    56041 ggaaaaagat tttttagatt atctagaaaa aaatagcaca ctctaataac ctaaaccttt
    56101 ttaaacttta atatataaat acctgattta gaatttattt ttatgcttaa tatattatca
    56161 ttaaaggttt tcgacataac tgttatttga ttaaatatag ggtctattat agtattacct
    56221 tcttcaagtc ttataagact tcttgggtct atgcttttat tatatgcctt aactccaatt
    56281 ttatattttc tcaatttaac aacatattcg gacatagaaa aaataaaatc tatattatct
    56341 tcatttaatt tactatcttc acccaaaatg gataatatag atttttttat gctattaatt
    56401 ccttcatttt ctacagttct atcttccata tatacatcat atagaacatt taaaacattt
    56461 aataaaatac tttcttcatc ataatcttca gatttcaaat caatgtactt atcaattaat
    56521 tgtattgctt taatccttgc catctgaaac tttacaacat ctgatttgtc taattctata
    56581 gaaaatccta ttatcttgtc tttaatactc tgtagtaatt cgacatactc tattttacta
    56641 ttatttataa gattgtgcat aatggaatta tacaccacag aacctaataa ataatcaaat
    56701 aaaagttctt cttttttaaa ataaataaca tactttttta ttagtttaat aacctcatct
    56761 tcactttttt gaatattagt atttgataaa attagtggta ataaaatatt taaaaaattg
    56821 atatttccta ttttttctcc ataaaatcta tagtaaataa gttcatctct cagtctattt
    56881 tcgtctatat acacacatct ttgcgtttta tcatctatta aggattttgt atttattata
    56941 gttaaatctt taagagccct acttgcacta ctaaaagaag atgtgtagac tttttttagt
    57001 aattcgtcat tcataaatat atcctttctg aaaaattgaa tgtaaataaa ttgttattaa
    57061 aaaaaaagag acagcccgtt taagaagctg tcatatatag ggggatagta gtgtacaaaa
    57121 atcattgtaa tctactatct atatattatt atacgatact acttttttat atgtaaagca
    57181 tcagttttcg acatccctat attttacagt tatacaaatt gcgtactaag ttcaatttta
    57241 ttctttttat ttcaacattt tatcatgcta cattctttca ggagcgtgca ttcccaacaa
    57301 tttcagagaa ttttctaaag tttgttttgt tgctaaaact aatgcaagtc ttgcttttct
    57361 tatttccaaa ttttctacta aaattggatt gtcatgataa aatttattaa acgcttgagc
    57421 taaatctagc ataaatctag tcactatatg tggctcattt ttctttagag caagtaaaat
    57481 attcttattg aatgattcta taactctaag aacttctgca ctatcttcat cagctaatag
    57541 actataatct atatcagaag tcacttctac ttctgctttt cttaaaactg cacagcatct
    57601 agcatgtgta tactgaacat atggtccagt ctcaccatca aaactaagcg ttctttccca
    57661 agagaaggta tagtctttaa ttctactatt agataactct tggaatacta ctgccccaac
    57721 acctacttgc ttagatattt catccacatt tttagcattt gggtttttag ctagtataat
    57781 ttccttagtt ttatctatag cttgatttaa agcatcttct aaaaatacta ctcttccttt
    57841 tctagtcgac atagtacctt cttcaagtgc aaccatacca aatccaacat gaattaaatc
    57901 ttttgcccaa tcatatccca taagctcaat taccttaaac cattgttgga aatgtaagtt
    57961 ttgttgagaa ccaactacat atatacactt gtcaaaatca taagtttctt ttctatatat
    58021 tgcagcagct aaatctcttg tcatatataa cgttgagcca tcattcttag taataagtgc
    58081 tggtggcatt ttatactctt ctaaatcaac aattcttgct cctttagact cttttaaaag
    58141 atttttctct tctaatagtt cgataactct gttcatttta tcagaataaa aactctctcc
    58201 tgctaaagaa tcaaattcta tatctaaaag tttatacact ctgttaaact cttttaaact
    58261 ctcatttcta aaccattgcc ataattcttg tgcttcttcg tcaccatttt ctaatttatt
    58321 aaaccatgct cttgcttcat cttccatttc tgggtgttgt tcagcttcat catggaatct
    58381 tacgtataat tttagtaatt ctggaattgg attagactct actacttctt tttctcccca
    58441 ctttttaaag gccactatta acttaccaaa ctgagttcca tagtcaccta aatgatttat
    58501 tcttatagtc ttgtatcctt gcgaatcata tatcttatat aaagcattac ctataactgt
    58561 agttcttatg tgccctatat ggaaaggttt tgctatattt ggagaagaat attcaactat
    58621 tacagtttta tcttttccaa aatcactatg tccgtaattt tctttttcat ctaaaacttt
    58681 tttaattaca gtttctgcta attgagattt atttacaaag aaatttacat atccgcctaa
    58741 ttgtattact tttgttatct ctccacttgg ttctatactt tccgccaatt cacttgctat
    58801 catgtttgga gcttttctaa acactttggc taatttaaag catggaaaag catagtcccc
    58861 catatcctta tttggtggaa cttctataag tgcctctatc tcttctttcg ataaatcttc
    58921 tattttttct ttaaggcaat ttgatattgc caccttaaaa tcttgcattc tgttgctcct
    58981 ctctacattg ttcctggttt atattgtgct tatctcttta tgttaaatca aatttcgtgt
    59041 tttttcaagt atttcattac agtgtaaaac actttagtaa ttatgttttc tgtatatatt
    59101 aaaaatacta taattgcagt ttttaatcac actattattg attttttata agactttttt
    59161 ctattttatt gtttcttgta ctatccagtt gactttatat acttttttta atcttttatc
    59221 tattttcata cgtcaatgct aattttatat acttttcttt taatctttta ttaattttta
    59281 tacatcaatg ttaactttat ataagttttt ttaatctttt atctgttttc atacatcaat
    59341 gtcagcttta tatacttttt ttactttttt atctattttt atacatcaat attagcttta
    59401 tatacttttt ttaatctttt atctattttc tataaatctt tttcactctt aacttttatt
    59461 ccttcttctt ctaatagcct tgctgttata cctttccctg gtatcttttt acctgaaaaa
    59521 ctaccatcat atattagatt acatccacaa gaggggctac cctcttttag tattgcttcg
    59581 ctacatacat ataaatttgc tatttttaga gtttcattag cccctcttac aaagttttct
    59641 gttacatcct tattatcttt agtaattact tttccatcaa ctatctcagc aggtggtctt
    59701 ggtgtagata gaccacctaa ctgctctggg catacaagtg taaattcttt gccttttaaa
    59761 tattccttga ctttttcatt atcattatta tcgccactat atttacaatt tattcctatt
    59821 aaacatgcag ataccaatat cataaaaatg tgctcctata caaattctta atatattctc
    59881 atacaaatta ctaatgtatt cttatacaaa ttattttaat ttaacaattg ttactcctgc
    59941 tccaccttct ccatattgac cacctctttg tgattttaca tgtctatttc ttcttaaaat
    60001 gtcttgtagc cctgctttta atactcctgt acctattcca tgtattactg ttacagactc
    60061 taaacctgcc acatatgcat catcaagata cttttctact tccattatag cttcttctag
    60121 gtttaaccct ctaaggtcaa cttcattttt tacactacca gattttgacc ttataatgtt
    60181 tctcgttgat ttagttacat ttgttgaaac atctttgcgt gttttttgta aagacttaaa
    60241 aggtagagtc atcttcatta taccaatttg cactactgct tcttttctct ttttatctac
    60301 tgaaactaca ctaccatttt gatttaaagt tattacctta acttcttcac ctggctttaa
    60361 gtcttttatc tccttatttg atacctttgg aactatcata ctttttactg ttggctgaag
    60421 acttcccata gagctggtca attcttttct aagttcttct atcttacgat tcttttcttt
    60481 tgaagctctt tcttgttcaa gacttctaag ttcttttatt ataatatcta cttcttcttt
    60541 agcttgtctt ataattgaaa atgcttctga tttagctttt tcaatcatct tatctctttg
    60601 actgaccaat ttttcaagct tttcgtcata ctctactttc agtttttcaa tctcttcttt
    60661 taatctttca gcttcttctc tatcttctac tgctttgatt ctattctttt caacattctg
    60721 taacacatct tctaaagcta tgttttctgt atttatatat tctttagcac gtgaaatcac
    60781 ataatcactt agcccaagtt ttctagaaat ctcaaacgca tttgattttc cgggaacacc
    60841 tataagtaac ttatatgttg ggcttaaagt ttctatatca aattcaactg ctgcattttc
    60901 tacacctggt ttagtaagag cataattttt aagctcacta tagtgagtgg tagctataca
    60961 tttagctcct acagaattta tatcttctaa tactgctatt gcaagggcag ctccttcaac
    61021 agggtctgtt cccgctccta actcgtcaaa tataactaaa gagtctgctg ttacgttttg
    61081 taatatagat actatattcg tcatatgaga tgaaaatgtt gatagactct gttcaatact
    61141 ctgctcatct cctatatctg caaatacatt atcatataca cacatactac ttccataatc
    61201 agcaggtata tgaagtccac tttgtgtcat aagtgcaaat agacctacag tttttattgt
    61261 aacagtctta ccacctgtgt ttggacctgt tatcaccaat gtatggaagt ctcttcctaa
    61321 ataaattgta tttgctacta ccttcttttt atccagcagt ggatgtcttc cattttttat
    61381 gtttaaatat ttatcctcat ttaaagttgg ttctattcct ctcatctgta tagaaagttt
    61441 tccctttgaa aatgcaaagt ccaatcttcc aagtatctct tgattagaaa ttaagtcttc
    61501 actaacttct ccaaccatag cagaaagctc tgataaaatt ctttctattt cttcttgctc
    61561 acctagtttt aactgtctca attcattgtt catctctact atagtcattg gctctataaa
    61621 aagtgtcgca ccagaagatg actggtcatg cactatacca gctacttgcg acctatattc
    61681 agattttaca ggtactacaa atctatcacc tcttagagaa attattgcat cttgtaagta
    61741 cttttgataa gtagttgaac ttattattga atttaatttt gacctaatgg attgattttt
    61801 ttgggctatt cttcttctga tatctctaag tgtacttgaa gcattgtcag atatttctac
    61861 ttcactaatt atagcattgt atatctggtc ttctatgtcc ttgtatacat atagagaatt
    61921 tgatagagat tgtattattg gataattaaa atcttcttca tcactactag ataagctatt
    61981 tcttaatctt ctagccactc ttaaggtatc tgctatcata atcagacttc ctgggtcaag
    62041 agaagcacct atataagctc tctttacctt atcttctata tcatgaatac cttccaaccc
    62101 tactgaacct ctctttataa taattgattg agcttcacta gtttcttcta gcatagattt
    62161 cacttctaca aaatcagtat ttggtactaa attttctata tattttagac ctaaagaaga
    62221 tgaggctttc ttctttaaca aatctattat tttattgtat tctagaactc ttaacgattt
    62281 ctcattcatt ttctatattc accatcctcg atattttaac ttttttattt aattttaaaa
    62341 atgtatgttt tcaagcattt ccaataaaaa ctgcaagttt tatttctttt tcatttctaa
    62401 aattattata ttttttctct tttagatata ttttacacat attttgtatt atactatgta
    62461 tgtagctatc tatatattat acataaacat gctttatttg taatataatc tgttaaaaaa
    62521 tcttctttaa tatttttaaa ggagtttatt ttagcttata aaaaaaaata gctacttata
    62581 tagataaatt aaggaggaac atgagtcatg ggaaacgtat tagaaggatt aaaaccagaa
    62641 agtgttttta aaaactttga gaaaatatct caaattccta gaggttctgg aaatgaaaaa
    62701 ggcataagtg attttttact tagctttggt aaaaatttag gtcttgaaac tattcaggat
    62761 gaaagcttaa acataataat aagaaagcct gctacaaaag gatatgaaaa ttgtccaggt
    62821 gtagttcttc aaggacatat ggatatggtt tgcgaaaaag aaaagaatgt tgaacatgat
    62881 tttcttaaag accctataaa attaagaatt gatggtgaca tgatatatgc tactggaact
    62941 actctaggag ctgacaatgg tatagctgtt gctatgggta tggctatatt agaagataat
    63001 actttagagc atcctgcact agaagtttta gttactgtta atgaagaaga tggtatgaat
    63061 ggtgcagatg cactagaccc tagcttaatt aaaggtcaat atatcctaaa tatggactcc
    63121 gaggaggaag gatacttatt agttagctgt gctggtggta aaacttgtgt tgttagttta
    63181 cctgttgaat acaaggaagt taaaggtgac aaacaaggat tattagttga ggttactgga
    63241 ctattaggtg gacactctgg tatggaaatt gttttacaaa gagcaaatgc taacaaagca
    63301 atagctagag tattaagtgt attgaatgtt gattatgaat tagcttctgt tgatggtggt
    63361 actaagcaca atgctatacc tcgtgaagct aaatgtgtaa tagctgttaa taaagctgat
    63421 gttgaatctg ctaaaaaaca aataaatgac attttaactg catttaaaca tgagtttact
    63481 acttctgacc ctggcatgac ttactcagta gctgaaactt ctgtagacaa agttcttact
    63541 aaagattgta aagaaaaagt tgttcaaatg tcttgcttaa ctcctcatgg agttcaatca
    63601 gtaagtcttg atatagaagg attagttgaa agttctacta acttcgctat aatcgaaact
    63661 aaagaatcta ctattgaatt tttaacttct gttagaagtt ctgttatgtc tattagagat
    63721 gaaatcgctg atagaataag acttttggct caagctttag gtgctaatta cgaccttata
    63781 gctcaatacc cagcttggga atttaaaaaa ggctcaaaat tagaaaaaat atgcagtgaa
    63841 acttatgaga aattaactgg taaagttcct actgttatgg ctctacatgc tggtcttgag
    63901 tgtggattat tacttgataa acttcctcat gctgaagcta tctctatagg accagatatg
    63961 tttgatgttc atactccaaa tgaacatgtt agtatccctt ctgtagctaa tgtttgggat
    64021 tatgttattg aaatattaaa atctatgaac caatattaaa tttaagtttt gattctaagt
    64081 agattttaat tttaagtaat ctatataaaa ttaactctgt aaattaatac tactgttagt
    64141 ataatattta ctttcaatat taacttatag aaataaagcc taatgataga taataacatg
    64201 tatcattagg ctttattgta atttaaatat tattaagctt tattctgttt taaaattaat
    64261 tcttatacta ttttaaatct atttcttcct tgtctcttag aatcataaag agcattatct
    64321 gctatttgat acagttcatc atactcttga ccaggctttg ctataactac ccccatacta
    64381 atagataaac tttctccatt tggcaactta atgagatgaa tatgattgta tacatcatta
    64441 catttattaa taagttcttc cttatttatt atatcattga tataaacact aaattcatct
    64501 ccacccaaac gacctactat atcatttttt gcaaaacttt catcaagtac ctttgcaaca
    64561 tcaataagta ctttatctcc taacaagtga ccataagtat cattaatcga cttaaaataa
    64621 tcaatatcta taatcaataa agcaccattt ttttcagttg ataaattcaa caaattttgt
    64681 gcagttaatt ttctaatata tgctgcatta taaatattgg ttaaactatc cctttgagcc
    64741 ttatctaaca atctattctt ttcctctttt tcagaatcaa tattacatat tttcccgatt
    64801 acatacattg gtatttttct atcattataa attatttttg aagtaactct aatccaatga
    64861 aaagtatcat caagcataat agaatacatt tcagttgttc cattttcttg tgattttatt
    64921 acatcaaaaa actttttatt atattctttc cttattttat ctctttcatt ttctatattg
    64981 ccattttcac tttccttatc atttttatcc aaaccataat aagtctcaat acccttttct
    65041 ggcatagaaa ttatcaattt atcttgctta taatcatact caaaaaagta ttcacttgtc
    65101 atttcataca actgagtatg ttttttgagt tctaaagata tcttctcgtt catacttgta
    65161 cgagtatgca aaccaaatgc cataaatcca ataatacaaa ttaataaact ggctacaata
    65221 aaaatagtct caattggatt ctgttgcata atataaagca acgagaagtc ttgtctatat
    65281 gtagtatttt ggtagacaat tgattgcatt ttttctgatg aaatacttat aatagcttta
    65341 ttaaatatat tcaataattc ttgtttatct tttttaatta taccaaaaca aatactcttt
    65401 gctttataag tttgaggaac tagacgcaca tttttatact taggttgatt catatagtac
    65461 tgcatagaat aaccttcgcc ataagtgtaa tctgcatagc ctttattaac agcttctata
    65521 cactcttcta aattatcata ccaaactata ttctcttcaa actgaccata ataattaata
    65581 cctttggcta gagctacacg ttttccatct aaactattct catttaattt attattaatc
    65641 atcatagtat attgagtaga aagaaaaggc cgagacataa ctatattatg ttcacgagag
    65701 tattcataat cataaatcat tcctgctaca atatcaattt cacgcttttt agtcatgttt
    65761 tctaaatctt cttgagattt tacaggcaca agttcaaatt ctaagcctgt tttatcacat
    65821 atatgattta acaaatcaat tgatatacct cttaattcac ctgtatcttt atctatattt
    65881 tgaaatggtg gtttgtttaa aagtactcct acttttaatt tttctgtctc tttaatgtaa
    65941 tcagtttctt catcagataa aaacatttga ttgttctctg gtgtaaagta tttttcatat
    66001 aaagtagttg aaaaatatgg gtctttctgt tcaatattat taattgctga gttaagcttt
    66061 tgagttaaat ttgtactacc tttaggagta atgaaataaa atggttttga tgaaaatttt
    66121 gctacagtac gtacaccttc tagtgggttc atactaacat taagaagtac atctgcttta
    66181 ccactcttta gtgcttctag ttgctcatca tttgttttac aatataccaa ttcaggtgac
    66241 ataagattca ttttacaata ctcatctaac tctttaagtc tagtatcaga attttctatt
    66301 acagcaatac ggattttttt cacatcttgt gaactcatta tgttatcaat atctgtattt
    66361 tcatataatg cttgtagtac tgtatttgct actccataac tatttcctgc ataattatat
    66421 attttttgca ttggctcatt gtataacata cctcccatta tatcaatttc accattttct
    66481 aacatcttca ttaattttgt aatagattca tttatctctc caggaacttg aacaaattca
    66541 taattccatc cagtatactg tgcaatttct agtaaatatt catatgtata cccaaaatag
    66601 ttaccttgct catctatttc agttaaacct ttttgaatag gataagcaac acgaatggta
    66661 cgattatcat tacctatagc gttgatacca aaagaaaata tgcaagaagt gaatatagat
    66721 gtaattacaa taattaataa aagtaatttt tttaaattca ctacacaatc ctccatatag
    66781 ttaagattta cagtataaat tactgcataa acttgtagac atgtaaaaat agattagtat
    66841 ataaaaggat atataaatct acttagaaat ttaaatatcc ttaatatata caatatattt
    66901 aaattataac tcatttatat gtctattaca acaaatttac cctgctaaat acctactcta
    66961 tatcccataa ttttacaagt tgtagtgaag tttaagttct atctcacctc aaatttagca
    67021 gtgtttctat ttacagttct tttatatttc acatctggat aacccaatat aaatgaagtg
    67081 ataacagctt cgttctcgct cattcctaac agttctttta tctttggctc tacatttgat
    67141 gccataacaa aaaatccatt ataacaaatt cctaatcctt gagaatatgc cataagttcc
    67201 atattagatg cagccagtcc tccatctaca taagcacttc cacctagaga catatctcct
    67261 acaactacca taagtagtgg ggcatcataa aataaagaat catttccgtt ctctttatat
    67321 cttttgtaca ttttcttaaa tgtattttta tagatagaac gtacagggtc attgtcatca
    67381 gtatctaatg ccaaattata aagaccttgt attgccagtt ctttcacttc ttttagcttt
    67441 tcttttacta atatgtatct tataggctgt ctattaccac ctgttggtgt gtatctacca
    67501 gcttccacta tgttttttat tttttcttct tctacctcta tatctttata ttgtcttata
    67561 cttcttctaa atttaataaa attcaataac ttttcgggtt ctaattcaaa actttcacga
    67621 ttgtattcta ttacttcatt tttatcaaat ccttgcattt ctattgcatc tgttgggcaa
    67681 attgccatac agtgaccaca gttaatacag agaatatttt taacctttgc tttatcatcc
    67741 ttaactttta ttgcatttac aagacagtct gattcacaca tcccacagcc tacacatttt
    67801 tctaaattta tgtctatcat tccaaattat tcctcctttt aaaaaattta ctttagtata
    67861 aaacaaatag ttgtctaagt caatttttat ttttttaaca atatactagc cttaactaca
    67921 ctttaatgga gaaaaatata cataaattgg aacttaaatt agaacttatt caaagtaaaa
    67981 gataaaagaa gtagatgtct aaaagtgaac tttttagtta attagatatt ctttattgta
    68041 tgaaatcaag tggatattta tcatttcaac aataaaaaag gcttacctct attttggata
    68101 tgtccctgtc aagtagacag actaaaaaaa ggctatcttt atgcactgag atagcccctt
    68161 ttcccaacta ctattgttta tcttaatatt agtcaataaa tttaatttgc tataacctgt
    68221 aagttacatt ttaatttgat gtaactttaa attacatttt taagtaatat ctttaaatta
    68281 tattcttaaa atatatcatt aaactacatt ttaattaatc taaaaactat atcttaatta
    68341 atctaagaaa cttacattat aatcttttag tttcccatta tcatatacaa tctttgcact
    68401 aaagatattt ccagtcttag cccaatttaa gaacagcctg tcaccttccc acatatttaa
    68461 atcgaatatt ttatcattat caacccaaat taaatttcct tctttacaat catttaaatc
    68521 tttttcagat aattcacctt caaactcatc tgagaaatac aaaaacatat accaatcttc
    68581 ttcatcttta aattttggaa aagttatcaa ccctttatat gacaatgatt taagagtaag
    68641 accagtctct tcttttactt ctctaattac acattcttct ggagtctctc cttgctcaaa
    68701 ctttcctcca actcctacat acttaccttc atgtatatca tctttctttt tatttctgta
    68761 caacatcaaa gtcttgtcat ctttttctat ataacaaata gttgtaagtt tcatgtatct
    68821 cgccttgctt tctctttata ttctcaacta attattttca gcactttcaa gcacatcact
    68881 tatattcttc attgcattaa gagaaagctc tgcaaattca tcaaatggaa tccctatatc
    68941 ttctatactt ctcattgcat ctctatttac atttgaagca aatctcgttt ctttcattct
    69001 tttaataaca gacttagtct ttacactggt aagtttttta tctggataaa ctaaagctat
    69061 agcagttata aacccagtta tagggtctgc tgcatatatt gctttttcca ttaggctagt
    69121 tcttgtaact cccattccat catgatgacc aagtattgct ctatacattt cttcgtcacc
    69181 gaagttttct tttttaagca gttctactgt tgttgtagca tgtccctcac atatttctcc
    69241 tggctttctt ccaactatat ctgaatccaa atcatgtaaa agacctgcca ctgcccactc
    69301 ttcttctttt tctggctcta gtcttttggc taattctctc ataacagctt caacagcata
    69361 acaatgtttt ctcattctat cagtttctaa atacttaaat aaaatttcta aagcttcttc
    69421 tctcattttc accaatcccc ctgttttttc acatatatga tatttttata tattaataca
    69481 cataaattca agtttttata tatattcatg tatatttttc atgttatatc tattctacac
    69541 ctttatagta gtgtcctgca actgttccaa tttcatctaa tttcttatac aagtctgtag
    69601 ccctttttcc aagtttataa ttattactca atacctcaat gtgagcttca ataacctttt
    69661 taaccatctc actatcttca actgtaaaat ctatcctaaa tacatctatt ccaatttcct
    69721 ctaactcata tagattgtcc aacatacaag taaccttcga attgtaaatt gtacttctac
    69781 agaatatatc ttgtgttatc ctataacttg catccgtaaa gtctcttaaa gcataactac
    69841 tttccttaca tttagcaact ctcttgtcct ttttacaatc cctaacaact actcccatag
    69901 gacagtattc tgtaaccatc ataggagcat atccatatat tactgcttca atttctacat
    69961 ctgtatattt taaagtttgt ttaatttcat ttaagtttag ttcttgtgac aaacaaactg
    70021 tacttgctcc ctcttctttg aagtggttta tactttcact attgaatgca tttaagtagc
    70081 aatctatatt taagtctttt ttcttaaagt attccataga gccccagtta ccaatttgaa
    70141 ccttattaat tccttttata ttatccaata tctcaaattg cttgtactct ttatttctaa
    70201 caatccttgg tgctgaatag attaattttt tgtcagtgac tgctgtatta gagtggttct
    70261 tattcaaagt atctatttta cttgtatttg acaaagtata tatgttattt gtatttgaac
    70321 ccattagttc tttaggaata cctaaagatg aagaagtata taaatccata gaaatacttg
    70381 aatctataga aatacttgag accatttgta aagcttttga taatgtattt atatcttcat
    70441 aatagattaa atctacatta taatttaata ctgcttctaa ttgctccaag ttcttaactt
    70501 taactctaat cttaggaact gtatcaagtt ttttctgttt atttactcta tttggctggt
    70561 attttaatct attcttctta aattttctat cttttatctt tattctttct tcatctaaaa
    70621 gctcaataca atcccttctc atctgattta acacacttat aggcatactt acaccttcat
    70681 ccaggtcaat ttctatctct tttaacttat atggtgtatt tccaagcttt tgaatttgtg
    70741 tttctatttt ttctttactt aaagccacct ttatagcttt ttcaactatt ttgtctcctt
    70801 ctaccaccga tttatttcca caaaaatcac ttataaaaag ctctggcttt ttacctagtt
    70861 taatactcac ttttgcacgt atattagttt ttatgaactc tttatcttgc tgaaatgtag
    70921 cttgtactct atccatcaat tcgctgtcag aagtcttata tataagctga tttctctttg
    70981 cttctccaac aaaatctaat tctatcaaat cccctttatg tgctattgta gttatctctc
    71041 catcctttat tattcttcca atagtacctc cacctaagtt aattccatca cctttcttaa
    71101 gcgtattttc tagcattact ctaagtcttt tgttctttct attataatct aaaacttttc
    71161 caatatatag cccttgatta ttaggtctat tagagttcat tatatcctta ccaacttcac
    71221 ctaatatata tccttttgta aactttctat taaatatggt atacaaatta tttattattt
    71281 cctcagaaat acttattttt ttcttatcta caaattcatt aatcgcttct ctatatcctg
    71341 aaaccacagt agctacatac tctggctttt tcattctacc ttcaattttt aaagataata
    71401 cacctgcttc aattatttta tccaaatctt ctatagtact taaatctcta ggactcaaca
    71461 aataatctcc attacttttt actacttctc ctgtatttat atctatcaac tcatactttt
    71521 gtctacatgg ctgtgcacac ctacctctat ttcccgacct attgcctatc atactactca
    71581 taaggcattg acccgagtaa catacacata aagctccatg tacaaaaact tctatatcta
    71641 cagttgtatt ttcacaaatc cattttatct catcaacatt taattctctt gctaaaacaa
    71701 ctctatcaaa ccctacactc tgaaggtatt ttacatcttc taaggaatgt gctaccattt
    71761 gagtgcttgc atgtagttca aagtcaggta atagtttttt tataagtctt gccattccta
    71821 tatcctgtac tattaaagca tcaatatcta tatcgtataa aaattttaca tactctacaa
    71881 aatcttctat ttcatactgt tttatcaatg tatttacagt aacaaagact tgcactcccc
    71941 ttatatgaga gtatttaaca gcctcaatca gctcatttct atcaaagtta tttgcagatg
    72001 ctctagcact aaagtcttta ccacctaaat acacagcatt tgctccgttt tgaactgctg
    72061 cttttaatga atcaaaagag cctactggtg caagtaattc tatttttttc atatttttct
    72121 cctctattta tacttgttag gttaatataa ttatatctat aacttaaata tttgtattaa
    72181 atttatatat ttcttactat attttaaatt cttcaatcaa tttata
//
LOCUS       DAMJYX010000010        68678 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJYX010000010 DAMJYX010000000
VERSION     DAMJYX010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.010"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(130..324)
                     /locus_tag="PC479_000706"
     CDS             complement(130..324)
                     /locus_tag="PC479_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803289.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="PC479_000707"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="PC479_000707"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2803290.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="PC479_000708"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="PC479_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2803291.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="PC479_000709"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="PC479_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2803292.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="PC479_000710"
     CDS             complement(3870..4640)
                     /locus_tag="PC479_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2803293.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="PC479_000711"
     CDS             complement(4659..5249)
                     /locus_tag="PC479_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2803294.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="PC479_000712"
     CDS             complement(5432..6400)
                     /locus_tag="PC479_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2803295.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="PC479_000713"
     CDS             complement(6393..7370)
                     /locus_tag="PC479_000713"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2803296.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="PC479_000714"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="PC479_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2803297.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="PC479_000715"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="PC479_000715"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2803298.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="PC479_000716"
     CDS             complement(11260..11688)
                     /locus_tag="PC479_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2803299.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /locus_tag="PC479_000717"
     CDS             complement(11705..12511)
                     /locus_tag="PC479_000717"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2803300.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="PC479_000718"
     CDS             complement(12713..13198)
                     /locus_tag="PC479_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2803301.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="PC479_000719"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="PC479_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2803302.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="PC479_000720"
     CDS             complement(14053..14733)
                     /locus_tag="PC479_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2803303.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /locus_tag="PC479_000721"
     CDS             complement(14717..15607)
                     /locus_tag="PC479_000721"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2803304.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="PC479_000722"
     CDS             complement(15725..17275)
                     /locus_tag="PC479_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2803305.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDNIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="PC479_000723"
     CDS             complement(17369..17635)
                     /locus_tag="PC479_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2803306.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="PC479_000724"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="PC479_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2803307.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="PC479_000725"
     CDS             complement(18727..19665)
                     /locus_tag="PC479_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2803308.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="PC479_000726"
     CDS             complement(19775..19936)
                     /locus_tag="PC479_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803309.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="PC479_000727"
     CDS             complement(19946..20443)
                     /locus_tag="PC479_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2803310.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="PC479_000728"
     CDS             complement(20558..21268)
                     /locus_tag="PC479_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2803311.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="PC479_000729"
     CDS             complement(21444..21974)
                     /locus_tag="PC479_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803312.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="PC479_000730"
     CDS             complement(21991..22221)
                     /locus_tag="PC479_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803313.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="PC479_000731"
     CDS             complement(22236..23630)
                     /locus_tag="PC479_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2803314.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="PC479_000732"
     CDS             complement(23756..25009)
                     /locus_tag="PC479_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2803315.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="PC479_000733"
     CDS             complement(25060..26214)
                     /locus_tag="PC479_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2803316.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="PC479_000734"
     CDS             complement(26774..27343)
                     /locus_tag="PC479_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2803317.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="PC479_000735"
     CDS             27546..27773
                     /locus_tag="PC479_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2803318.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="PC479_000736"
     CDS             complement(27992..28735)
                     /locus_tag="PC479_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2803319.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="PC479_000737"
     CDS             28903..29775
                     /locus_tag="PC479_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2803320.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="PC479_000738"
     CDS             29775..30440
                     /locus_tag="PC479_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2803321.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="PC479_000739"
     CDS             30461..31318
                     /locus_tag="PC479_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2803322.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..34020)
                     /locus_tag="PC479_000740"
     CDS             complement(31474..34020)
                     /locus_tag="PC479_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2803323.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVISKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34020..34706)
                     /locus_tag="PC479_000741"
     CDS             complement(34020..34706)
                     /locus_tag="PC479_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803324.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="PC479_000742"
     CDS             complement(34789..35778)
                     /locus_tag="PC479_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2803325.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="PC479_000743"
     CDS             complement(35765..36451)
                     /locus_tag="PC479_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803326.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36951..40157)
                     /gene="carB"
                     /locus_tag="PC479_000744"
     CDS             complement(36951..40157)
                     /gene="carB"
                     /locus_tag="PC479_000744"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2803327.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40350..41399)
                     /gene="carA"
                     /locus_tag="PC479_000745"
     CDS             complement(40350..41399)
                     /gene="carA"
                     /locus_tag="PC479_000745"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2803328.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41656..44862)
                     /gene="carB"
                     /locus_tag="PC479_000746"
     CDS             complement(41656..44862)
                     /gene="carB"
                     /locus_tag="PC479_000746"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2803329.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44915..45958)
                     /gene="carA"
                     /locus_tag="PC479_000747"
     CDS             complement(44915..45958)
                     /gene="carA"
                     /locus_tag="PC479_000747"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2803330.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="PC479_000748"
     CDS             complement(45984..46700)
                     /gene="pyrF"
                     /locus_tag="PC479_000748"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2803331.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47076..47711)
                     /locus_tag="PC479_000749"
     CDS             complement(47076..47711)
                     /locus_tag="PC479_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2803332.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47735..48649)
                     /locus_tag="PC479_000750"
     CDS             complement(47735..48649)
                     /locus_tag="PC479_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803333.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49250..50479
                     /locus_tag="PC479_000751"
     CDS             49250..50479
                     /locus_tag="PC479_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2803334.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(50684..53065)
                     /locus_tag="PC479_000752"
     CDS             complement(50684..53065)
                     /locus_tag="PC479_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2803335.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(53037..53762)
                     /locus_tag="PC479_000753"
     CDS             complement(53037..53762)
                     /locus_tag="PC479_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2803336.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(53752..54207)
                     /locus_tag="PC479_000754"
     CDS             complement(53752..54207)
                     /locus_tag="PC479_000754"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2803337.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(54489..55670)
                     /locus_tag="PC479_000755"
     CDS             complement(54489..55670)
                     /locus_tag="PC479_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2803338.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(55660..56352)
                     /locus_tag="PC479_000756"
     CDS             complement(55660..56352)
                     /locus_tag="PC479_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803339.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(56486..57202)
                     /locus_tag="PC479_000757"
     CDS             complement(56486..57202)
                     /locus_tag="PC479_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2803340.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            57327..58061
                     /locus_tag="PC479_000758"
     CDS             57327..58061
                     /locus_tag="PC479_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2803341.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            complement(58346..59197)
                     /locus_tag="PC479_000759"
     CDS             complement(58346..59197)
                     /locus_tag="PC479_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2803342.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(59223..60161)
                     /locus_tag="PC479_000760"
     CDS             complement(59223..60161)
                     /locus_tag="PC479_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2803343.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            complement(60264..60914)
                     /locus_tag="PC479_000761"
     CDS             complement(60264..60914)
                     /locus_tag="PC479_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2803344.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            complement(61063..61251)
                     /locus_tag="PC479_000762"
     CDS             complement(61063..61251)
                     /locus_tag="PC479_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2803345.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            complement(61358..62542)
                     /locus_tag="PC479_000763"
     CDS             complement(61358..62542)
                     /locus_tag="PC479_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2803346.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            complement(62684..63601)
                     /locus_tag="PC479_000764"
     CDS             complement(62684..63601)
                     /locus_tag="PC479_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2803347.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            complement(63623..64351)
                     /locus_tag="PC479_000765"
     CDS             complement(63623..64351)
                     /locus_tag="PC479_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2803348.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            complement(64547..64747)
                     /locus_tag="PC479_000766"
     CDS             complement(64547..64747)
                     /locus_tag="PC479_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803349.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            complement(64872..65252)
                     /locus_tag="PC479_000767"
     CDS             complement(64872..65252)
                     /locus_tag="PC479_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2803350.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(65525..66883)
                     /locus_tag="PC479_000768"
     CDS             complement(65525..66883)
                     /locus_tag="PC479_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2803351.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            67331..67783
                     /locus_tag="PC479_000769"
     CDS             67331..67783
                     /locus_tag="PC479_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF2803352.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(67940..68557)
                     /locus_tag="PC479_000770"
     CDS             complement(67940..68557)
                     /locus_tag="PC479_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2803353.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT        21598 a        12629 c         7444 g        27007 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgttgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttgc tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttc ttataactat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaatta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaagcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtacctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag ttaatacatt cctaacaata
     6661 tctactctgg ataacatata tccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggggct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctataatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgct ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcacc atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa gccttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcagtttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaataata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatatttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttccca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatattatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcatt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggca caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaagaaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgccata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccga ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gtattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaaataaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtatcc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gagttcatta atcttctact agcttggtct cttgtcaatc cactgatacc
    25261 atccttaagt ttaaatgaaa ctacgcctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgtgatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tctccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatgcct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcaat acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcattt tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agatatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagagaca cctatgaatc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttgtg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc ccctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttatc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gttcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agtaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttccac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactcccc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 catagaactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 cagtgcgtaa ttttgctctc taatgcgtaa cgtcatttta gttatttcac tttgtagaat
    35521 agaaagctca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc agttgctgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggtt cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc ttcgctgttc cttctgtagc aacaaaagta
    37261 tatcctaatt ctttcatatc ttttgctatc tctataaact catctttatc atgattattt
    37321 acagtagcaa gtactactcc tctttcatca gacatatgtc tacctgctgc caagaaccct
    37381 ttatataagg cttcttctaa attttctcct acacctagga cttctcctgt agacttcatt
    37441 tcaggtccta gacttacttc aactttagat aatttagaca ttgagaatac tggtactttt
    37501 actgatacta attttggctc tttgtaaact cctgtaccat atcctaaatc ctttaattta
    37561 gctcctaaca tacacttagt agccaagtca actattggta ctccacttac tttacttata
    37621 tatggaactg ttctactagc tctagggtta acctcaatta tatataattc attttgaaat
    37681 tctataaact gtatattaac cataccaagc acattaaggt ctaaagctat ttttttagtg
    37741 tattctacta tcttttcttt tatttcatca gatatatttt gacttggata cattgttgta
    37801 ctatctccag agtgaactcc agctctttct aaatgctcca ttataccagg tatcaatata
    37861 tcttctttat cacatatagc atctacttct atttctctac cagttagata tttatctata
    37921 agtactggat ttttatggtc tctatcaaat gcatcatcca aatactgaat tagtttttct
    37981 tcgttataag ttatttccat accttgacca ccaagtacat aagatggtct aactaatact
    38041 ggataaccta actccttagc aatttcaaca ccttcattag ttgtccatac accttttcct
    38101 tttggtctat ttatatctag tctctctagt aaatcatcaa atttttctct atcttctgct
    38161 gcatctatat ctctaaagtc tgttcccagt ataggtatat ttttttcatg taaaaactta
    38221 gccaatttta tagctgtttg tccaccaaac tgtagtatta caccttctgg tttttctttt
    38281 tctattatat ttaaaacttc ttcttctgtt aatggttcaa aatacaactt atcagatgta
    38341 tcaaaatccg tacttacagt ttcagggtta ttgtttacaa ttatagtttc tattcccatt
    38401 tttcttaaag attttacaca gtgaactgaa caatagtcaa attctattcc ttgacctatt
    38461 cttattggac cagaacccaa tactacaact tttctcttat cactaactac tacctcatca
    38521 tattgttcat aagttgaata gtaataaggt gatagagctt caaattctcc tccacatgta
    38581 tcaaccattt tataaactgg ttgtatatta tataaacttc tcaattcata taatttttct
    38641 gggcttatct tcattaagtc agatatacct ttatcagaaa atcccttttt ctttaattca
    38701 tgtaaatatt ctttatctaa gtcttctatt tttagaactt ttaacttttc ttcttgttct
    38761 actatccatt taaatttatt tataaaccac ttatcaacac cagttatttc tgatatcatt
    38821 tctactttat agcctcttct tatcatttca gctaagtcaa atagtctctc atcatcaggt
    38881 actactactc tagcctttaa ttcttctaat gttctacctt ctgatggagc atgaaccaat
    38941 gaatattttc ctgtttcaag tgatcttata cctttaagaa tagcagcctc aaagttactt
    39001 cctatactca tgatttcacc agttgccatc atcttagttc ctaattttct attggcatgt
    39061 ctaaatttat caaatggcca ctttggtatt tttactacca cataatctaa tgttggctca
    39121 aaacaagcat aagttttttt agttactgca ttttctatct catccaaagt ataacctaaa
    39181 gctatcttag ctgctacttt tgctattggg tatcctgtag cttttgatgc tagggctgat
    39241 gacctactaa ctcttgggtt tatttctatt atcgcatatt ctaaactatg tgggtttaaa
    39301 gctatttgaa cgttacatcc accttgaacc tctattgcat ttattatgtc tattgatgcc
    39361 tttctaagta gttgatactc ttcatcactt agagtttgac ttggagcaac aactatactg
    39421 tctcccgtat gaactccaac tgggtctaca ttttccatat tacatacagt tatacagttt
    39481 ccatttccat ctctcattac ttcatattct atttctttcc aaccttttat actcttttca
    39541 agaagaactt gtccaacagg acttagctgt agtccatgtg aaagtatttc tcttaattct
    39601 tcctcagtat cagctatacc tccaccagtt ccacctagcg tatatgctgg tcttacaact
    39661 actggatatc ctatcttatt ggcaaactca agacctgctt ctaaatttgt aactatatca
    39721 ctaactatta ctggttgatt tatttctttc attacttctc taaataaatc tctatcttcg
    39781 ccctttttta tagattctat agatgttcct ataactttta cattatattt atctaatata
    39841 ccagcatcat gtaattctac agctaagttt aatcctgtct gaccacccat acctgcaagt
    39901 aaactgtctg gtctttcttt ttctattatt ttttctataa attctattgt taatggttct
    39961 atatatattt tatctgcaat ttctttatca gtcattatag tagctgggtt actatttact
    40021 aatacaactt caattccctc ttcttttaat gcttggcagg cctgtgttcc tgaatagtca
    40081 aactctgcag cttgacctat tattattggc cctgacccta atactaaagt tttctttata
    40141 ctatctaatt taggcataac tctttctcct tatctaaaca attttcacag ttcttatctt
    40201 catcatctat acaatcaact ttaattactt tattttatct taagttcaaa tttactcatc
    40261 aatacatcta tttaatacat ctatgttaat taaaccaaac tttttgattt aagttattct
    40321 tgttttttag ttagctattc taatgttgtt tattttacta attctaaaaa tttatcaaaa
    40381 atatagtcat tatcttttgg tcctgggcaa gcttcaggat ggtattgaac actgtataca
    40441 tctagcttct catgtcgcat tccttcaaca gtattatcat ttaaatttat atgtgtcact
    40501 ttcatttgtt caggaacttc tgaaacataa taaccatgat tttgagaagt tataaatact
    40561 tttccttctt ctaaatcttt aactggatga tttccacctc tatgcccaaa tttaagttta
    40621 gcagtttttc ctccaagagc taaagctaaa agttgatgtc ctaagcatat tccaactata
    40681 ggttttttac caataagagc ttttatattt tctataacat cttctaagtc ttctgggtct
    40741 ccaggtccat ttgacaagaa tattaagtca gggtttatac tcaatacatc ttctggagat
    40801 gttgtagctg gaaatattgt tatatcacat ccacgagcta taaatgatct taatatattt
    40861 ctttttactc caaaatccat aacagccact tttggaccat ttccttttat atgctctact
    40921 tcttttcttg taacagttct aactgcttct gtatttgaaa acttatccaa tttatgttta
    40981 acatcttcta atttactgtc ttctaatgta attatacctt tcattgttcc attatttcta
    41041 agtatcttag tcaaagctct tgtatctatt ccttcaagtc cgataacttt attttgcttt
    41101 aaatattggt ctatatcaat ttcacatcta aaattacttg gagaatcact tttctccctt
    41161 actatgaatc cctttacttg aactttttca gactctgctg atgataaatt tataccataa
    41221 tttcctacaa gaggataagt catagtaact atttgaccat agtaagaagg gtctgttaaa
    41281 acttcaccat atcctatcat cgaagtatta aatactactt ctcctacact ttcttctaaa
    41341 tatccaaatg cctttcctat aaaaactgtt ccatcttcta atattaacct tgctttcata
    41401 ctacagtacc tcctataatt ctctttagac aaactaaaga acattaacta aatataagct
    41461 tttaaatatc tatatctatt tttcactttt acattctagt tttatactta tttacattta
    41521 ttttttatct ttttctatta tactacaaat ttcttaaaaa aaagaagtta tctcaaaagt
    41581 aaaaactcct agaaaaaata acctcttttt tttatttaat aattaaattc ttaaactata
    41641 caatcaactt aatttctatt cagctatatt gtaaactttt aactcatctt tatttaaatg
    41701 tttcttctta acttctacta aagcttttag tgtatccaaa gatgtcatta tttcagtaga
    41761 aaattcaata gctgttcttc ttatcttaaa tccatctctt gtagagtcat ttcctttagt
    41821 tggtgtattt attactatat caacttgttt atttcttata gcatctaata tgtttggtct
    41881 agattcttct actctattta ctatatcagc ctctattcca ttttctctta agctattagc
    41941 tgttccttct gtagcaacaa aagtatatcc taattctttc atatcttttg ctatctctat
    42001 aaactcatct ttatcatgat tatttacagt agcaagtact actcctcttt catcagacat
    42061 atgtctacct gctgccaaga accctttata taaggcttct tctaaatttt ctcctacacc
    42121 tagaacttct cctgtagact tcatttcagg tcctagactt acttcaactt tagataattt
    42181 agacattgag aatactggta cttttactga tactaatttt ggctctttgt aaactcctgt
    42241 accatatcct aaatccttta atttagcccc taacatacac ttagtagcca agtcaactat
    42301 tggtactcca cttactttac ttatatacgg aactgttcta ctagctctag ggttaacctc
    42361 aattatatat aattcatttt gaaattctat aaactgtata ttaaccatac caagcacatt
    42421 aaggtctaaa gctatttttt tagtgtattc tactatcttt tcttttattt catcagatat
    42481 attttgactt ggatacattg ttgtactatc tccagagtga actccagctc tttctaaatg
    42541 ttccattata ccaggtatca atatatcttc cttatcacat atagcatcta cttctatttc
    42601 tctaccagtt agatatttat ctataagtac tggattttta tggtctctat caaatgcatc
    42661 ttgtaggtat tgagataatt tttcttcgtt ataagttatt tccatacctt gaccaccaag
    42721 tacataagat ggtctaacta atactggata acctaactca ttagcaactt caataccttc
    42781 gttaagtgac catacaccct ttccttttgg tctatttata tctagtctct ctagtaaatc
    42841 atcaaatttt tctctatctt ctgctgcatc tatatcatca aaatcagttc ccaatatagg
    42901 tatatttttt tcatgtaaaa atttagccaa ttttatagct gtttgtccac caaactgtag
    42961 tattacacct tctggctttt ctttttctat tatacttaaa acttcttctt ctgttaatgg
    43021 ttcaaaatac agcttatctg atgtatcaaa gtctgtactt acagtttcag ggttattgtt
    43081 tatgattata gtttctatgt ccatttttct taaagatttt acacagtgaa ctgaacaata
    43141 gtcaaattct ataccttgac ctattcttat tggaccagaa cctaatacta tcaccttttt
    43201 cttatcacta acaactacct catcatattg ctcataagtt gaataatagt aaggagatat
    43261 agcatctatc tcacctgcac aagtatcaac cattttatat gctggtttta tattgtatag
    43321 acttctcaat tcatataatt tttctggact tatcttcatt aggtcagata tacctttatc
    43381 agagaatccc tttttcttta attcaagaag atagtcttta gttaaatctt caattttcat
    43441 aacttttaat ttttcttctt gctctactat ccatttaaat ttatttataa accatttatc
    43501 tatacctgta atttgttcta tcatttctac tttatagcct cttcttatca tttcagctaa
    43561 atcaaatagt ctctcatcat caggtactac aactcttttc tttaattcct caatgcttct
    43621 ctcctcagat ggtgtgtgaa ctaatgaata ttttcctgtt tcaagtgatc ttatcccttt
    43681 aagaatagca gcctcaaagt tacttcctat actcataatt tcaccagttg ccatcatctt
    43741 agttcctaat tttctatttg cttttttaaa tttatcaaaa ggccattttg gtatttttgc
    43801 caccacataa tctatagttg gttcaaaaca agcataagtt tttttagtta ctgcattttc
    43861 tatctcatct aaagtataac ctaaagctat ttttgacgaa actttagcta ttggatatcc
    43921 tgtagctttt gatgctaaag ctgatgacct actaactctt ggatttatct ctataactgc
    43981 atattcaaaa ctatgtggat taagtgcaaa ttgaacgtta caaccacctt gaacttctac
    44041 tgcatttaat atatctaaag atgctttttt aagcattgca cattctttgt tacttaaagt
    44101 ttgagttgga gcaacaacta tactgtctcc tgtatgaact ccgactgggt ctatattttc
    44161 catgttacat acaactatac aatttccctt tgaatctctt attacctcat attctatttc
    44221 tttccaacct tttatactct tttcaattaa tacttgtcca acaggactta attgtagacc
    44281 atgtgaaagt atttctctta attcttcctc attatcagct atacccccac cagttccacc
    44341 tagtgtgtat gctggtctta caactactgg ataaccaatt gtaagtgcat aatcaagacc
    44401 tgcttgtaaa tctgtaacta tatcactaac tattacaggc tgatttattt ctttcattac
    44461 ttctctaaat atatctctat cttcaccttt tttgatagat tctactgaag tacctattat
    44521 ctttactcca tatttatcta gtatgccttt ttcataaagt tcaactgcta ggtttagccc
    44581 tgtttgtcca cccatacctg caagtaaact gtctggtctt tctttttcta ttattttttc
    44641 tataaattct attgttaatg gttctatata tattttatct gccacttctt tatcagtcat
    44701 tatagtggct ggattactat ttattaatac aacttcaatt ccttcctctt tcaaagactg
    44761 gcaagcttgt gttcctgaat agtcaaactc tgcagcttga cctattatta ttggtcctga
    44821 ccctaatact aaagttttct ttatactatc taatttaggc ataaattttt ctccttatct
    44881 aaatggtttt tactatctgt aaataatact aaatctatat taatgctaag aatttattaa
    44941 atatacaatc taaatcttct tttgttgtgt attctggaat gtattgaaca ctatatattg
    45001 gtaattcctt atgtctcatt ccttctatag tattgtcatt taaatttatg tgagttactt
    45061 ctgtattttc tggcatttct gaaacattat atccatgatt ttgagaagtt ataaatacct
    45121 ttccagtttc taaacattta actgggtgat tacctcctct atgtccaaat acaagctttg
    45181 aagtctttcc tcctaatgcc aatgctaaaa tttgatgtcc taaacctatt cctgtcaatg
    45241 gctttttgcc tattaatttt tttatatttt caactatatc atttatatcc tctggatttc
    45301 ctgggccatt agataataag attaagtcag gatttatatt taaaatttct tctactgtag
    45361 tcattgctgg tactatagtt atatcacagt tacaagcctt aagagactct agtacactat
    45421 ttttaacacc aaagtctata actacaactt tagctccagt accttttata ttttcaattt
    45481 cttttcttgt aacatttttt actgaatctt tgttacaatg ctcttctaat ttagactgaa
    45541 catcttgtaa tgatacattt tctaaagtta taattcctct cttagttcca ttgtttctaa
    45601 taattttagt taatgctctt gtatctattc cttcaagtcc tattacctta ttttgcttta
    45661 gatatgtgtc tatatccatt tcacatctaa aattatttgg gttatcactt ttttctctta
    45721 caataagtcc ttttacttgc accttatttg attcaacatc ttctaaattt attccatagt
    45781 ttcctataag tggataagtc atagtaacta tttgtccata ataagttgca tctgtcaata
    45841 gttctccata cccagccatt gaagtattaa atacaacttc tccaatagct tcctttagat
    45901 atccaaatgc ttttccttca aatactgttc catcttctaa aattaatttc cccttcatgt
    45961 aagctacctc ccaaatattt tatctaagca ttttctattt ctcttacaat attaatagct
    46021 gattctcttg gattttctgc ttttgttata ggtcttccaa caactaaata atgagcacct
    46081 ttttttattg catctgatgg agttacaact cttttttggt ctccaacctc tgctgattta
    46141 ggtcttattc ctgggcaaac tgttataaaa tcagttccac atgcttctac tattttatct
    46201 acttcttgcg ctgaacaaac aaccccatct attcctgatt cttttgccag tttagctctc
    46261 tttatagcta actcttctgt tgtcatatta catcctatgt cctgtaaatc ttggttgctt
    46321 aatgaagtta aaactgttac tccaactact aatggctttt ctatatttaa tctttctgct
    46381 tcttccctag caattagtgc acattgtctc attccttcca tatctgatac atgtattgtc
    46441 attaaccaaa cattatctct cactgcagct ttcactgcac ttttcatagt atttggtata
    46501 tcatggaatt taaggtctaa aaatattttt ttcccttttt ctttaagata atctattgtt
    46561 ttaccttttg tggcaacgta ttgctctagc cctaccttaa atatgtctac actatcttct
    46621 aatctgtcta ttagttcttt agctttatca aattcgtcag tatcaattgc aactatcaat
    46681 ttttctttac catttatcat ttttacatct cctttttaat aaagcctaaa taatcataaa
    46741 aaaatcctca caccaaaaga ggcataagga ttttatctat acataactct attcattagc
    46801 tatgagatat ctttcccagt ctctctggac tagtttaaaa gactttatta tatttaaatt
    46861 tttattttat actcatcaaa aacctctaga agatttacta gaggttatat actaacatta
    46921 tctataaccc tttagtctct ctggactact ttaaggaatt tagctgtaat atttttcttt
    46981 atttatataa ctttatgtat ctataggata atttaaagtt ttatcttttt tataattaaa
    47041 ccactaattg ctttttatgt caataagtct ttgaattatt cttcatcttt atcaaacaat
    47101 tttttctcaa aatatgattc tgcattatat attgcagcaa ctggattatg cccagttact
    47161 ctatctttga caactaaagt tgttatagga gcttccatat atttcatggc taaagtatcg
    47221 tgaccaacac ataatccaag aattacatta aggtcaacct tagcttcatt taaaaataaa
    47281 gcttgtccta ttggattgca cattacctca tctgaacact ttgatattgt ttgagatgct
    47341 tctagtccaa ttgtactctt aggcattgcc ccatttttac atataacaga aaatacttca
    47401 aacccatgat gttcaaatat tttttgcact gtttgcattt ctctttttag tccaatacaa
    47461 aatgctatgc caatcttttt ataaccacac tttttagcaa actctataat ctcttttagt
    47521 ctagtatatt gacaataccc ctctgcttca actaatccag cattataagc tatttttcta
    47581 ttctcttctt cttcataaag ttgctttgct ttttgttgta gttcaatttc tctagttgga
    47641 catatcttca ttaatttatc catatttcca ttagaacagc ctctaagatt acattttcca
    47701 cacttataca tatccaatat ccccttccaa aattttattc atcttgcgat tcttcttttt
    47761 ctagagttac attttcttct tgggtttctt cttctggttt cacatgctcg actacagtta
    47821 taaaatatga ttttcctatt ttattcctta taggaagttt ttcatatgaa acatctattt
    47881 tattttttat acaagctata tcaaaatgtc ttaaggctac acctaagcta accttattca
    47941 tatcttcaca gtgtttctta ggtctctttt caaataaatg aagacctcgt tcatccttat
    48001 ggaaatacca cggttgagaa ttttttacag atggagctct tcttgtcaat tctaagatat
    48061 ctttccactt tctattccta tttatcttat ttatcatatc ttttactggc tttctatcag
    48121 gtctagcata tctcgtccta aacaactctt cattttttgc tggatatcca aatgctatta
    48181 ttatataagg attttctatt ttgttttcat catcttcttc tgtttttatt ccagctagat
    48241 aatctaaatc atcttcctca tcttcttcaa aattcttatt cccaatatct acaaattcca
    48301 agatatcttc tctctttaag tttccttcca accagcaagt agctattccc aatgttgtaa
    48361 ggcgcaaaac tacaccttct gtagcaaatc ctatattctg taaatagtcc tcacctttgt
    48421 ctgatgtaac tactatataa tgaggtgcct ttactttaca ttccttcccc atcaaaaaat
    48481 gtattatatg acctctatca acaacatgtg ctttaatatt taaatcttca tttaagtatt
    48541 ctaaattttt acaacttctt tttatttctt ccatcaatgg cttttttatc tttttactgg
    48601 aaaaatttct tacagatttt ctataaaaaa tggcttcata aagctccatt tatgccctcc
    48661 taactgaaat cttctgacgt ataaactcta taaatttata taaatacttt atatttttta
    48721 tagttttatt tcctttttcc tcctgcccta ttttgaaatt cataagaata tctactctag
    48781 tttaatatag cattcaaaag gcttaaattc taccttatca atgtctatac attagcataa
    48841 tttttttatt tgattttcta tgctaattta aataacaaca ctagctatta cctttttaat
    48901 taagctttgc cagaaaaatt ttttttcatt tataaatatc tatctcatta ttatattata
    48961 agtttttttc ggtattcttg tatagctttt cactaaaaaa gtatttttat ttctacttta
    49021 atattattat caacaaaaaa catactactt aaataagttt gttatcctca taatttgttt
    49081 taattaaaat atttttttta tttctcaatc tataaagtat aaatattttg aatatgaccc
    49141 aaaatctaag taaagttata atattttata agttgttcaa tattaaaaac ataagtaatg
    49201 ttataatact ttataagtta ttcaatatta aaaatatagg aggataaata tgaatttaaa
    49261 tagaaattta gaaaaatacg ctgaattagc aataaaagtt ggagttaaca tacagccagg
    49321 tcaaattcta cttataaagt caccaataga atgtgctgat tttgcaagaa atgcacttaa
    49381 agaagcatat aaatgtggtg ctaaaaatgt ttatatagag tggtctgatg aagaaagtac
    49441 tcttattaaa tacttatatg ctccagatga agcatttcat gagttcccca aatggacagc
    49501 agaacaatat gtagatatag caaaagaagg tggggcattt ttatcagtct atgcccaaaa
    49561 tccagattta ttaaaagatg ttgaccctga aagagttgca aattttcaga aggcatctgg
    49621 aaaagcttta aaagaatggc gttcttacac tctaactgat aaatgtaaat ggagtatagt
    49681 ctctgtgcca acaaaagatt gggcaaaaaa agtgtttcca aatttatcag aagataaagc
    49741 aatagaaaaa ctgtgggatg ctatatttaa atgttcaaga gtagatggtc aggaccctat
    49801 aaaagcatgg gaagaacata atgaaaatct taaatctaaa atggactttc taaataaaaa
    49861 taactttaaa actttaaaat ataaatcctc aaaaactgat ttgactctag acttgccaaa
    49921 gggtcatgtt tggctaagtg gagcatccaa agaccctaat ggaataagtt ttaatccgaa
    49981 tattccaact gaagagattt ttggaatgcc acataagttt aaagtaaatg gaactgttta
    50041 tagtacaaaa cctcttgttt atggtggaaa cataattgac aatttcttct taacatttaa
    50101 agatggtaaa ataatagatt tctctgctga aaagggacta gatacactag aaaaacttat
    50161 tgaaacggat gaaggctctc attatttagg agaagttgct cttgttccat acaactcacc
    50221 aatatccaat acggatataa ttttttataa tactctatat gatgaaaatg cttcttgcca
    50281 ctttgcaatt ggttctgctt ataagacttg tctggaaggt ggaaacaatt taaaagatga
    50341 agaattagat gaacatggtg taaatgacag tttaacacat gtagatttta tgattggttc
    50401 tgctgatatg gatattattg gtgagactta tgatggaaag caaatacaga tttttaaaaa
    50461 tggtaattgg gctttttaac atatgatttt aactaaaaag ggctgatgga agttatacca
    50521 gacttcccag ccctttttaa tgttgatgat aaatattttt tatattaatt aatctcatta
    50581 ttatagatta attcctattg ttttaaacat aagtatctaa aacttatatt tatattatat
    50641 ataaaatatc ctatttagta aaacctttat tttaatttat atgctaaaaa tttaactttg
    50701 ctatttcaac agcagacttt gcatcctttg aataataatc agcacccatt tcttcagcgt
    50761 attcttctgt caaaactgca cctcctacaa atacctttgc atttatctca ttatctctca
    50821 gtgcctgaat tgtatctttc atactttgaa cagtcgtagt cataagtgca cttaatccta
    50881 ctaattttat atttctcttt ttagcaactt ccactacttc ttcaataggg acatctttac
    50941 ctaaatctat aacttcatat ccataatttt ctagcattat ttttactata tttttcccaa
    51001 tatcatggat atcacctttt actgtagcaa ctattatttt tcctttggat acattattgc
    51061 tacttttact taataaagtt tcctttattg tatttaatga aacttttacc gtttctgctg
    51121 attgaatcaa ttgtggtaaa aatatatctc cactatcata tctttttcct actttatcaa
    51181 gagcaggaat taaaatctca tctaatatat aattttcatc cttctctttt aaaagattca
    51241 gtgttacatt ttttgcttca tcttttaatc ctctttcaac taaaatatcc aagggcaaat
    51301 ctttatcaat ctttgtagat gaatccgatt tttttgaatt atttatattg ctatattgat
    51361 ttatatagtt tatacatcct ttatctgtgt tatttaatat tttaaaagaa ttgatagctt
    51421 ccatcatact atattcattt ggatttataa ttgctaaatc aagccctgcc cctaaagcta
    51481 atgttagaaa agaagcatta agtgctttcc tatttggaag accaaatgaa atatttgaaa
    51541 cacctaatat agtttttact cctagtgtct ttaccattgt tatagctttt atagtttcta
    51601 ttgcttcttc ttgttgtgct gaaacagtta atgataaaca gtctataaat atatcttttg
    51661 gttttatccc ataagaaact gctctattaa ctatcctttt tgctatttca aatctttctt
    51721 cagccttttt aggtatcccc ttttcatcaa gtgtaagtcc aaccacacat gaaccatact
    51781 ttttaactat tggtaatata ctttctaaag actcctcttt gccatttaca gagtttacta
    51841 ttgttctacc attataatat ctaagtcctt gctctaaggc ctctacattg gaagaatcaa
    51901 cttgtaaagg tgtatctact actgcttgaa tctctcttat taattttggc attagttttt
    51961 tttcatcaat ctctggaaga ccaacattta ctccaagtat ttgtgctcca gcatttactt
    52021 gttctaaacc taaatttata gcataatcaa cattttcatt ttttaatgct tcttgtagag
    52081 attttctacc tgtgggattc aatctttctc caacaactgt aggcgattgt acttccacaa
    52141 attttgatgg tgaacagact acacatttat caattttctc gatacatccc tttgtaacag
    52201 aatttatatt ctcagatatt tcttttataa aacttggatt cgtaccacaa catccaccta
    52261 ttatagtagc accaacttca acgaactttt taaccccttc aaaaaattct tttgcatcaa
    52321 catcatatat agcctctcca tcaactatat ttggaagacc tgcatttgct tgaaccataa
    52381 cagggactgt agctcttgat gctatttttt caactattgg taaaagttgt ttaggtccta
    52441 atgaacaatt aactcctatt gcatctacac ctagaccttc tatagtagcc accatacatt
    52501 ctggcataca tccagtaaaa cttcttccac cttcatcaaa tgtcatagta cagaaaatag
    52561 gtaaatcaga gttttctttt gctgctaata cagctacttt agcttcatat aaatccatca
    52621 tagtctctat tactattaca tcaactccag atttaacgcc ttgtagtact tgtcttttaa
    52681 atatttcata tgctttatca aaacttaaag tacccatagg ctctagcatt tctcctatag
    52741 gaccaatatc taaagctaca tatcttggtt tagtattatc tacattttct atagcttttc
    52801 tagccaccaa aacagcattg tcaatcactt cttctacagt atacttacta tctaatttta
    52861 gttcattaca tccaaaagta tttgtaagaa ttacattcga acctgcctct aagtatgctg
    52921 tatgtatttc tataagttta tctggatttt gaagaccaaa tacttctgga ttttcaccta
    52981 actttaaccc tttttgttga agcattgttc ccattgcacc atcaaatatt aatatattat
    53041 ttttcagata ttgtcttatt tccacaagag ttcgctcctt ttctatatgt acagtttcta
    53101 tagtttttac aattttcaca cgacttttct ttcttagatt tatttattct tgtattagaa
    53161 atacctataa tagccactac tgactttctc ggtatcataa ttccattaga agtaattgtt
    53221 agacctattt gtttttgagc attcaaaacg tttaatatat ctatattttt ttctattggt
    53281 aaatctccat atcctgggct atatcgcata gttataaatt tatcttgctt taaaaaaacc
    53341 ttttctaaat tattttgaac aatgtcacac acttcttcta ttgctgttgt tccacatgaa
    53401 tcaattatta gtccttttgt taaatttgaa taagagtgtc gtcttatttc tttttctata
    53461 tctatcccta tagtagctgc cataattaca cactcattac attcttttaa aagttcatat
    53521 acatctttac tctctaatgt taaatttgtt ccctctaatt ctacaacatt atctttattt
    53581 attttaatag gataaatcct agataaatat ctaggattta ttttattttt agttatagct
    53641 acacattcat caatagtata tttaaggtct ttatctatct cttgacctct atattgtaaa
    53701 tatcttaaaa cttcactttg atttatgctt atatcttctc ttccaattaa attattctcc
    53761 atatatattt aaagaaaatc ctttatctaa aacatctttt gcatattcct ttgccccaaa
    53821 taaagataag tctctgtagt ttccacattc tatagctgta gctgctggca ttttatctga
    53881 ttcaagaact ttttttaatg cttcttctaa accaatctta actgttttgg catctacatc
    53941 tccccatagt attaaataaa atcctgttct acatcccatt ggagataagt ctattacacc
    54001 atctaatgta tctcttaaat aagtagctaa aagatgctct aaaccgtgca tagcagcagt
    54061 tccaaaactt tctacatttg gctgtaaaaa tctcaagtca aatttagtta ctttatctcc
    54121 ctttactcca tcaagtacac tacactttct tacaaaagga gcttttactt ttgtatggtc
    54181 tagcttaaaa ctttctactt tttccattat ttaatcctcc attatacttt aaaatttgtt
    54241 acattaattc taaaaaaaac tcttcctatc tattattgat aagaagagta tattatttca
    54301 agtcatctca tcgttgttag caggaccacc tgataaaagg ttggtatagg atcactgagc
    54361 taactctctc ccctaactct tgataagtat cttattaaac tgacttaaat tatatattac
    54421 ttttttattt ttgtaaacac tttattgtta taatatttat atcacataat cgtactttta
    54481 ttttataact aagctgattg tattattggc agttgtactt caaacacagt ttgatattta
    54541 tcgcttgaaa cagtaatttc tccatcatgt aaatttacaa tattttttgt aatcgcaaga
    54601 cccaatccag aacctccaac atttctattt ctcgattttt caactctata aaatctatca
    54661 aatatatttg gtaaatctaa cactggaatt ggttccccat aatttatcac ttttacaacc
    54721 gccatatctt cctttttctc tgtgactata tctacataat gcccatcttt tccatatttc
    54781 acagcattag ttataagatt ttcaaatgct cttaccaatt tatttggatc agcttttaca
    54841 attaattttt cttcattaaa attaattcta ctaaccataa attcttgttt aaaataaatt
    54901 tcaaattgag atactacttg acttaataac tctactaaat ttatttctat tttgtttaat
    54961 tttattgtat tattttgcat tttagtcaat tcaaacaaat catttataag aacatttaaa
    55021 tttttcgatt tttcatatgc aatatcaaca taatacatca tctgaacttc atctttatac
    55081 ttaccttctt ctatcactcc taaataaccc attatggatg tcaaaggtgt tcttaagtca
    55141 tgtgacacat ttgttattaa atctgttttt gtctgctgag cttgtctctc ttcaatagtt
    55201 atatttctaa gttgatggac tatattattg attttatgtg ctagttcatc aatatcatcc
    55261 ttttctttat cctttaatat atctaaatca tactcaatat ctatactttt ttctaagtta
    55321 ccttcatcca ttaaatttac agcactttta atgatttcta atttttgatt ttttctatta
    55381 gtctttctta taaaatctac aatttgctta tttctcttgt acgatatcat ggaataaatt
    55441 gctaatgatg tgattgtaaa taaaaatgga taaaataaat cataccctat aagacttctg
    55501 aaatctcttg cttgatatac aagttgatta actacagaca tatcactatt gacatataat
    55561 gattttatta tatacatact tgttaaaata agcattgaag ttaaagcaca gcttattata
    55621 agatctaata tgggtcgaaa catatgattt aataaacttt tatttttcaa ttttatatcc
    55681 tactccccat acagtatgaa ttattttttc tccatctgta tatttttcaa ttttatccct
    55741 tattctgctc atatgaacca ttacagtatt attagattgg taatatttct ctttccatac
    55801 cttttcaaat atttcttctg tactatatac tctgccttta ttatttgcta gtaaatataa
    55861 tatatcaaat tctcttgatg tcaaaattat ttcatttcca gccactgtta cactatgttt
    55921 ttccttatct ataattacta ctccaacctt tatgacatct ttatcttcat tattagcacc
    55981 actaaaataa tatgctcttc taatcagcga cttcactctc acagtaagtt caagtgggtt
    56041 aaatggtttt gtcaaataat catctgcacc agtcattatc ccttgtattt tatccatgtc
    56101 ttctgatttt gcacttaaca taagtattgg tatattcaaa ctttctctaa cttttctaca
    56161 aacttctatc ccatccattt taggcatcat tatatccaat atcattaaat gaatattttc
    56221 cttttttaaa atatctagag cctcttttcc atcttcagct ttaattacat tatatccact
    56281 atttaaaaga tatatctcta taaggtctct tatttccttt tcatcatcca caacaagaac
    56341 tgtattattc atttttacac atcctaactt aatctttgta tatagcttta ttcatataaa
    56401 attatactat attttagtta gtgtaatcta taaatttatt aatttctaaa tttccaatat
    56461 aatacaaatt atttacacat ttatattatt tattttgtaa atattcttct aaagttaatc
    56521 cttttgaata aatttcctcg gcagcatcct taccaacata tctaatatgc catggttcat
    56581 atgctgtacc tgttatatct tctttacctt caggatatct tattataaat ccaaacttgt
    56641 gagcattttc tgccaaccat ttagcttctt ttgtacttcc aacaaaccat ctttctggat
    56701 ttgttaaatc aatacaaagt ccagtttggt gctcactgta accaggcttt gctacatacg
    56761 catctgcctt ttctcgcccc tgagatttta cccttcttgt ataagtatcc agttgagtat
    56821 cataagatct gtatgctgaa gaccctaaaa actgtattcc ttctgaatta gcttgcttaa
    56881 ctaaatcttc aactgccttt gcaggttctc ctgccatttg tttttcttcc tctgttgctt
    56941 cttcaacaaa aggtatgttt accttagtaa tattttcagg agtatagttt ttgctaattc
    57001 catttgtctt gtttaccaat aatatatttg atatactatt tgagtgattt ttattttgag
    57061 aatttgcctc aacatcttgt tctgaatcat gttgagcagc tactattgga tctttatctt
    57121 tcttaagtaa tctatcttta gatataaata cacttataaa tactactaaa ataatggctc
    57181 ctattaattt aaattttttc ataattaaat cttctccttt ttgttatgtt tgctataatg
    57241 ttaattttag agtataagac ttaagtattt tataagtaaa tcttaaataa atcttaaatt
    57301 taatattttt aaaggatggt gataaaatgt ctatacaaaa acttctaagt aaagctcgta
    57361 aggctataca agattttgat atgatacaag aaaatgataa aattgcggtt ggactttctg
    57421 gtggaaaaga tagtttaact ctacttcata tacttaaaag ttatcaaaga ttttctccac
    57481 aaaactttga attgatagcc ataacattaa atactggagg tgttgataat tctccactag
    57541 ataaattgtg taaagaaata aatgttcctt tttatgaatt tcaaactgac ataaaagaaa
    57601 ttgtatttga tataagacaa gaaaagaatc cttgtgcatt gtgtgctaat ttaagaagag
    57661 gtgctttgaa cgataatgct aaaaaacttg gatgtaataa agtagctctt ggtcatcata
    57721 aagatgatgc aattgagaca tttttaatgt ctatgtttta tgagggtaga gtaaattgtt
    57781 tttccccaaa gacataccta gacagacagg atttaacagt tataagacct atggtttaca
    57841 tagaagagta tatgactaag aaaatttcaa aagattctaa ctatcctatt atcacaaatc
    57901 cctgccctgc aaatggtcat actaggagag agtacataaa aaatcttatt gctaatttaa
    57961 ataaagagat gcctgatttt aaaagaaatg tatttggtgc tttaaataat tctgagaaat
    58021 tgtttatatg ggataaagag aagataaaaa attttaaata ataattaaaa tttttaaata
    58081 aaaaagcaag attgtattaa ttgtaaataa tctttatata atctacacaa tcttactttt
    58141 ttatttatta tatgttatta tctttaatct atattttaat agttcttaat agtatttatt
    58201 ttgtctaata cattaattat aacttaaata ttttatatat taatttaatc tattaattca
    58261 ttcaatctac tggtttaatc catttttaca gtatatacat ctacatatta taaaaagcaa
    58321 aattatattt tctaaatatt ttatgttaga ttataatatt aaattctatt atctctggta
    58381 tattaaaaaa tctaaatggt aactttgtac ttccaagacc cgaactcaca tataaatgat
    58441 ttccatttat gtcataaaac cctttagtat atttccttcc atatggagga gtaactaatg
    58501 ctcccttaat aggtaatctt atttgaccac catgactatg cccagataga actaaattca
    58561 ttgtatcttt actaagcata tctactaaat ctggttcatg tgctaacaat aaattaaaac
    58621 ttttattatc tatttgactc tccagatatt ttatattagg atttccattt aatatttcat
    58681 caacacctaa tatattaata tatttatcat cttttaattt tattttttta ttttcattta
    58741 ctaaaagatt gaagttggaa tttttcataa tctgcctata atacctagga agtttataca
    58801 tataatcatg attgccaaat actgaaaatt ttcccaacct agcattcata cttgataaaa
    58861 tagttgtaat ttcatctaca cttggatttt tattggagta atcaattaaa tcaccagtaa
    58921 atactattat atctggattc aaagtgttaa tcttattagc tacttttttg agattcttag
    58981 ttgaataaaa actacctatt tgagtatctg aaatttgaac tacttttaca ctttcagatt
    59041 tactctcaat tgaatcattt tttatcaatg ctccattttc acttatatta tattttttca
    59101 caattaatag actcggctct atcgcaactg agtatataaa aatcaaaata aatattaaga
    59161 taaaaaattt taaaaaatac ttaatttttg agttcaaaat atctacctac ttatttattt
    59221 ttttaaacta tttgcattac agactcaact ttgttcattt ttctgtcatg catttttggc
    59281 ttatttgctg aaaaccattt atatgttgct gtaagacctt cttttagtaa cacgtttggt
    59341 atataaagcc catgttcaat aagcttattt atatcaagat taaagtctat atttctaaat
    59401 gggaaatacg ttctttctct aaactctact ttttccatat ctacatattt tattataggt
    59461 tctttgccaa taatttctcc acatgtatat ataaggtcat cccaagaaat taactgtgga
    59521 tttgtcacat tataggcctc tcttacatga ggatttttca ttatgctttc caataccttt
    59581 accaaatccc caatatatat aaattgatta ctaacttgtt ttccttttgg tactggtatc
    59641 ttttcatttt tttctatttt ttcaaagaaa taatactctc tatataaatt attattttca
    59701 ccatatatgt aagttggtct aataattata tatggtatag ggctatttat aataaaatct
    59761 tcagcctctt ttttatttaa cccatatttt ccccagtttt cattttcacc tttctcattt
    59821 tcttctttta tatttcttcc actatcttta tatactgctc ctgctgacaa tactatatac
    59881 ttttttaagt tatccatgct tataaaatca atcagattac ttacatcttc ttttgtatat
    59941 gctgtcatat catatatgta atcatatttt cttcctgtga taatgttttc catatctttt
    60001 cgaacttttc tatcacaaat tagatgttct ttaaaaccat tataatctat ttccttttta
    60061 ccatttgtaa gtatatcaat ttgataccca catttaatca agcgcttagc aagtgcagaa
    60121 cctatgaagt cgcttccacc cataattaaa atagatttca ttttaagtgc atcctcctct
    60181 tttttaaatg acttccttaa ctataatatc atatttttaa atatgcgaca ccattttgta
    60241 atatttttgt ctataaaaga tatttataac aaaatatctt ttatagactc tacattttcg
    60301 acaatataag taggttcact ttcactaatc tcttcaaaag aaccataccc atacaatact
    60361 ccaatagaat ctatacctat ttttttagct ccaattatgt catactttct atctccaacc
    60421 ataataactt tatctttatc ttttacatta cataggtcta gtacatattg tataacttca
    60481 tttttattca ctcttgtacc atcaagattg ctacctgcta tatatttaaa atatctatct
    60541 atatcaaaat atctaagtat agtttctgca aaaactgttg gcttagaagt tgctactaat
    60601 aatattttac catttttata gagcatttct aatatctctt tcatattctc ataaatttta
    60661 ttttcaaata tccctttatc agcaaaatat tctctatact tttcaacagc ttctttagct
    60721 tttttatctt caaatttata atactccttg aaagtatcat gtaatggtgg tcctataaat
    60781 tggtctaagt tttccaaatc ttcttttatg ccaaagctat ttaaagaata ctgaatagac
    60841 tttgttattc cttctttagg gtctgtaagt gttccatcta aatcgaataa tactatttca
    60901 taattttttt tcatatttca gtcctccata tttgtaaaaa gtaatatctt atatattaag
    60961 tttatctatt ttaaaaagct atagtaatag aaatttctaa tactatagct taagtaccaa
    61021 ttaaatctta tatatttatt ttaaaaataa atttaaacag ctctatttaa ctgttatagc
    61081 tgaaactgga catccttctt cggcttcttt acataaatct tgtaaatctg ctggtatttc
    61141 tccttcaatt gctactgaat gcccttcatc gctcatacta aaaacgtctg gacatgttcc
    61201 tatacatgct ccacaagcta cacatacatc cttatcaaca aatgctttca tactaaaata
    61261 cctcccctaa aattattatt ttttaattgt tgtttcctta gatatttatc caatatatac
    61321 caatttaaac atatatttat tattttatca ctattgttta gtttattata gtattttcgt
    61381 atccatgtct ataaacttca ctgcttttca tccatgattt tacagtatat tgcaatctta
    61441 attttttatt taaaacaagt tcattattat tgtagtcttt tataatatca ttacactcct
    61501 ccattgtaaa tattgtaaaa tcaccttctc caccaatctt tattctgcct ttatttccat
    61561 gtaaatcgaa aatctttgtt gggtattcag ttactttttc tatacacttt tctagggata
    61621 tcccaagttc cataatttta tttataacat ttaaaagaga atagtttacg tctttcatgt
    61681 tttttatatg catatcagta cttattaggt caggctctaa accttgcttt attgcttttt
    61741 taaaaacttt aaaatcaaat tggtccactc catgacatac atcaaacaat actcctctat
    61801 tctttgcatt tattactgat tttcttaatg tgccagattc tttaaggatt ccatttgtac
    61861 tatttccata taccggtgta accacatctc cttttcccaa aatattcatt acttcatcta
    61921 tatatgatgg atactctcct atatatacca ctaagggtaa atttaatttt ttagcaactt
    61981 gctttgattt cttaattata tctataccac tttcattaga ttgaaattta ctcgctttta
    62041 cttttatacc ctttatcttt tctatatttt tttctacaat tgactcaacc ttgtcaaaat
    62101 ttataacatc taaataagtt gattcattca atatatttag tccctcattt gtaacattta
    62161 aaaaagaata tacttctgtt ttacactcat ccatatactt ttttttaaaa tcatagtagt
    62221 ttgagggacc agctgtacct gcatcaaata taactgtaga acctttttca attccaattg
    62281 aatcagaatc catacctgta ggtaattttc tcttataaca atgggtgtga acatctataa
    62341 atcctgggga aatatacata ttatcggcat ttattatctc gaactcttta tttgtaagta
    62401 atttttcttt tcttattttc tctttaaggt tcaatccaat agcatatatt ttcccatgta
    62461 aaaccaagat atctcttttt tcaaatttga taccatctgt tggagatatt attgtcccat
    62521 ttcttataag taaatttttc atttattcca catcctaatt acaatataat ttctctaata
    62581 gcttttctta cataaagaaa ataagcctaa aaataaaaat attttagact tattcactat
    62641 attatactat attaattata ttaaattaaa taaaatcttt aatttacttt aagaagcaat
    62701 taagtatttg ttgctcggct tcttcttcag taagacctaa tgtcatcatc tttagtattt
    62761 ggtctcctgc aatctttcca atagctgctt catgtactaa aagagcatct ggatgatttg
    62821 cagcaatttc aggtatagca ctaatttgag aatttcccat aattattgaa tcacattgaa
    62881 catgtgcaga acaaagggaa tccccaataa cacaaggatt aaacttttgc ttagaattat
    62941 cttgcccaac tgtacgagaa actacttgag cagaagagtt ttcaccctct aagataacct
    63001 tcatattaga ttctgcaact tgattaccat gagttaataa tttctcaaat acaactattt
    63061 tagctccttt acctaggttt gcatccattt ctcttttagt tgaatctaca cccttaattt
    63121 gaattgtttc catttcacaa tatgaatcct catccatatt gacaatagta actggattta
    63181 aaattctctt tccttctcca tcaccttcac cataatgttt ttctacatat tttagtcgag
    63241 ctcctttacg taagaaaaat ctatgtatac cattatgttc actatcatca caaccacagt
    63301 tatgtatacc acacccagca attataacaa catctgcatt ttctccaatt tcaaaatcat
    63361 tatataccat atcctgtaca ccagcctctg ttactattac tggtatatgt acattttctc
    63421 cttttgtatt tggctttact ataatatcta ttccaggttt atcagtttta ggaattatag
    63481 ttacattttc agttgaatgt ctctctactc cttgcccatt tttacgtata ttgtatgctc
    63541 ctaatggagt tccacgaagt tcagcaactt tttctaaaag atttaaatct attttgttca
    63601 tttaatttgc cttcctttct aattaattca tgtctttcat taattcacaa cttgaattaa
    63661 cttgacacat gatttcaggt aatatagcct cttttgttcc atggcttttt actgaaccat
    63721 cttgtagaac aattatttca tcagctaact ctaatattct ttcttggtga gaaattatga
    63781 ttatagtctg attagactct tcatgtattt tcttaaaact ttcattgagt tttccaaagc
    63841 tccaaaggtc tatccctgct tcaggttcat caaatataga aacctttaaa tctctagcaa
    63901 aaagagttgc tatctcaatt cttttcattt cccctccaga aagagagtta tcaacttcac
    63961 gatttaaata gtcttttgaa cacaatccaa catctgtaag gtattggcaa catacatcag
    64021 tagacaatgg ttttccatga gcaagtgtaa gtaaattttc aactgtcata ccttttattc
    64081 ttggtggttg ttgaaatgcg tatcctattc ctttttttgc tctttctgta atactaagat
    64141 ttgttatatc ttctccatct aaaattatct gaccactcgt agctttttca attcccataa
    64201 taagcttagc aattgtagat tttcctccac catttggacc tgtgataaca ataagttttc
    64261 ccctctcaaa tgaaaggcta acatcattta ttattcctag ttcctcattg ttagactcaa
    64321 cattaaagga aagatttttt atttcaagca taatacccct cttttctata aaatattaca
    64381 ttaatatcca ttcttttatt atatcagtaa tttttatata tcagcaactc taaggagata
    64441 aaatgtatat taaataataa aacaaagtta ttgaaaagta aaaaagatga gttttatgta
    64501 aactcatctc agaaatttag tatatcaact tatatgtatt taaaatttaa ccttctatat
    64561 actctaatat tattttttga ttatctttta acataatttc ttctttttta gattctattc
    64621 cattcttttt tatctttacc tcagctacaa aggcatcatt accttcaacc atttctaata
    64681 gttcatacgc ttttttacga cctattttag ttgctatatc ttttagtact gccattttct
    64741 taaacatttt atcacctcta ctcaaaattt acacttaatt tttaattata taattctaat
    64801 cctagactag tttataataa ttattgtctc taattatatc agatattgag taaatttaat
    64861 actttgtaaa attatttatc ctctctgacc aaataatcat ttccatcttg gtctttaaaa
    64921 gaaaacatcc ttccatatgg catctccatt aattccccaa cctcaacctc gttatttgcc
    64981 atttgttcat aagttccatt aatatctctt gtacttaaaa ttattgatgg atgagataca
    65041 tttatctctt tgttttgctt catcattaga tttttatcat acaacacaaa tgttgtaaat
    65101 tcatttttac ttggtgctac ttccacccat ttcatatttg gacccatttc cttttcataa
    65161 gttactacaa agttcaattt ttctgtccaa aataactttg catcctcttg attatttaca
    65221 tagagagtta tttttcctat tttatttatc ataacaatac ccacctttat tattaaataa
    65281 atacctaata ttttttatat ttaataatat acccatatat attattttct atagatatat
    65341 atttctaata tttatcgcca aattatgtaa ttctgctcaa tatataattt ataatatcca
    65401 tactattata gcctttattt atgtaattta atcaccttat atatcatact tataattatg
    65461 ataataacta cataaaaaat cacttctaaa tagagttatc catctaaaag tgatttcata
    65521 tattctatgc aactttgtgt tttagccact ttgttcctct taatcttaaa caatataaag
    65581 tccctctaac aagccaatca gtatacatac ctatccaaat tcctaacact ccaaattcta
    65641 atacaacccc caatacgtaa cctgtaaaaa ttctaaaaat ccacatacct attactgcag
    65701 tcatcattgt atatctagta tcaccggctc cctttagccc tgctgataat acaaaggata
    65761 taggccatat taccatagct aaacaatcac ttcgaattaa ttgtgttgag atgtcaatta
    65821 cactagcatt ttgagtatac atacttgaaa tccagggtgc aattggtata aatatagctc
    65881 ctactataaa taaacaaatt gttgcaaatt tagttaaata taataaagta ctcttagctc
    65941 ctttaatatc tccacgtcct atatactgtc ccaccaaggt tgtagcagac aaagctaaag
    66001 cattcccaat tacatttatt atggatgcaa ttgacattcc tattgcattg gatgcaattg
    66061 ctgatgttcc catagtaact ataaatactt gaacaatcaa tttacctgca ttaaaaagta
    66121 gttgttcaac tccagctggt attcctatat taaatataga cttttgagtt tcaacatcaa
    66181 tcttaaatgg aaatatttta cccatagata taaattcgtt tcctttaaat aaagctctta
    66241 taattactat acatccaatt attctagcta tagcaataga tatagctgca cctgttatgc
    66301 cataggatgg tgtttgcaaa tttattccta gaggatttaa atcaatacca tatataagta
    66361 tatatcccaa tgttacattt actatattca taaacatagt cacatacata ggtttcttta
    66421 catctccaca ccctcttaaa ataccatttg ctatttgttg tgtcgcaata aatggatacg
    66481 taaataatgt aaactctatg tataattttg catttgattt taccaattct tctgcggaac
    66541 cataaaataa attaattaca ccagttctaa aaacccacat caatattgtt attgtaaagg
    66601 ataatattac tccagacact agagcctgct taatagtttc attcaccttt tcactatctt
    66661 tttttcctat ttgttgagct acaacaacag tagctccaac agatagtgca gcaaatacag
    66721 atataaatat attgtttatt gaatcaacca ttccaactgc agatacagct tcttccccaa
    66781 tatgacctgc catcattgta ttaaatactc ctaaaagcat gacaaacgtc tgctctatca
    66841 ttataggaat tgctaacttt ataacatttt tccttataaa catccctatt cctcttttcc
    66901 ctctttatat aaatttaatt tttaattagt ttatcgaact gtaaattgac aatgcaaagt
    66961 ttagaattgt attgttcgca ttataatata ctgctttcaa ataatataaa tcttgtttaa
    67021 tatcatttga acacagtata tctttataat tatcttatgt ttattatttt ttatggatac
    67081 cacatttaaa atatttatta atttaatata aatattttcc gttattatta aaagtagcta
    67141 tatttcgtta atattgtatc actatcgtgt agcaataaca ataagtacat tttatatatt
    67201 ttttataata ttatttaatc atccaaactt ttaccacttt ttcaatatat aaaatattac
    67261 actttagatg taacaaaatc gcatccattt aaatattgta ttttatagac taattttagg
    67321 atgtgatact ttggttaatt taaatgcaaa taatctaaat gtaaatatgg tttatcctat
    67381 ttcttccaca gcacccatac gtttcagaag atatactgtt tcatatttag aaaacatgga
    67441 aaatatttat ttaactatag caacatactt tgatacactt ggtttaggca caatgaatat
    67501 tgacaaagtt tatggtcaat gggcttggtt tactgataat atatatgatt taaatctatt
    67561 tgtttttgta ggaaattatc cttatcaaat tgctaaaaac agatatgata catttgtaga
    67621 tattttgcca gtagctatta cttctattgt caatggagat agagtatttt taatttcaaa
    67681 tccttgtttg ttaaattcaa aaattacagt tagatttata tctagtcatc ctactcttaa
    67741 tagaacagtt gattatggca atattagaga ttttattttt taaaactgca acatgatgtg
    67801 ctattaaaaa ataccctaaa tttaatgata ttattaaata actagaatat ttttaattgg
    67861 cacatcatgc ctagctatta taaattcata atattatttt acttaagata agtactgtat
    67921 tttatctatt tgtatatttt tatttaccta gtttctcagt tctatatcct atttctttat
    67981 tagctagctt ataaccttct tgtatcattt cttctctcgt taatgcactc cctaataaat
    68041 tgtcaatact cagttgcttt ctatcactaa aatacttatc caataatgat tcctctatat
    68101 cttcctgata tgccaaattt ccttcatgca ttatatattt tacatcaaaa ccttctttat
    68161 ttaatacttt tcccaccaag atacttctat gacatcttat aggttcttgc atagctccta
    68221 ataaaactat cctataaccc atttgacaac ccttttttaa tctttctatt ccgtttaaaa
    68281 aaatatcttc ttttgctact ttctcaaaat ctgcataacc ttcttgcgta tatacatctt
    68341 tattagttct tttagctcca aattcttttc ccatgtatat atataaaaat ccggaatttc
    68401 taagagattc gttaaatgcc tctttattgt attgaatatt atatttagaa tatggagttt
    68461 ctctaatgtc aactacacaa tttatgtcat atttttttat catattaata agcttatcat
    68521 atggataatt tgaatgtcct attgcgaata tttccatata aacaccctcc tattctttga
    68581 aattagtatc tatatatgta gatacccgat ttacttaaaa ttaaataaag tattacttta
    68641 aatacccaat atcttttttt cttgatattg tatatttt
//
LOCUS       DAMJYX010000011        63860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJYX010000011 DAMJYX010000000
VERSION     DAMJYX010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.011"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            325..3228
                     /locus_tag="PC479_000771"
     CDS             325..3228
                     /locus_tag="PC479_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803354.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            3261..3707
                     /locus_tag="PC479_000772"
     CDS             3261..3707
                     /locus_tag="PC479_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2803355.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            3830..4132
                     /locus_tag="PC479_000773"
     CDS             3830..4132
                     /locus_tag="PC479_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803356.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            4313..5719
                     /locus_tag="PC479_000774"
     CDS             4313..5719
                     /locus_tag="PC479_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2803357.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6063..7121
                     /locus_tag="PC479_000775"
     CDS             6063..7121
                     /locus_tag="PC479_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2803358.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            7220..7906
                     /gene="araD"
                     /locus_tag="PC479_000776"
     CDS             7220..7906
                     /gene="araD"
                     /locus_tag="PC479_000776"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2803359.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            7924..8571
                     /locus_tag="PC479_000777"
     CDS             7924..8571
                     /locus_tag="PC479_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2803360.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(8744..10111)
                     /locus_tag="PC479_000778"
     CDS             complement(8744..10111)
                     /locus_tag="PC479_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2803361.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(10301..10389)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10817..12937
                     /locus_tag="PC479_000779"
     CDS             10817..12937
                     /locus_tag="PC479_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2803362.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13041..14294
                     /locus_tag="PC479_000780"
     CDS             13041..14294
                     /locus_tag="PC479_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2803363.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(14448..15509)
                     /gene="ytvI"
                     /locus_tag="PC479_000781"
     CDS             complement(14448..15509)
                     /gene="ytvI"
                     /locus_tag="PC479_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2803364.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            15783..16523
                     /locus_tag="PC479_000782"
     CDS             15783..16523
                     /locus_tag="PC479_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2803365.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            16535..18313
                     /locus_tag="PC479_000783"
     CDS             16535..18313
                     /locus_tag="PC479_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2803366.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            18480..19397
                     /gene="pfkB"
                     /locus_tag="PC479_000784"
     CDS             18480..19397
                     /gene="pfkB"
                     /locus_tag="PC479_000784"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2803367.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            19434..21353
                     /locus_tag="PC479_000785"
     CDS             19434..21353
                     /locus_tag="PC479_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2803368.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            21949..23475
                     /locus_tag="PC479_000786"
     CDS             21949..23475
                     /locus_tag="PC479_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2803369.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            23503..24060
                     /locus_tag="PC479_000787"
     CDS             23503..24060
                     /locus_tag="PC479_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803370.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            24582..25619
                     /locus_tag="PC479_000788"
     CDS             24582..25619
                     /locus_tag="PC479_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803371.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            25730..26872
                     /locus_tag="PC479_000789"
     CDS             25730..26872
                     /locus_tag="PC479_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2803372.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
     gene            27326..28717
                     /locus_tag="PC479_000790"
     CDS             27326..28717
                     /locus_tag="PC479_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2803373.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            28970..30763
                     /locus_tag="PC479_000791"
     CDS             28970..30763
                     /locus_tag="PC479_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2803374.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(30844..32226)
                     /locus_tag="PC479_000792"
     CDS             complement(30844..32226)
                     /locus_tag="PC479_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2803375.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            32484..32831
                     /locus_tag="PC479_000793"
     CDS             32484..32831
                     /locus_tag="PC479_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2803376.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            32849..33883
                     /locus_tag="PC479_000794"
     CDS             32849..33883
                     /locus_tag="PC479_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803377.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(33932..34981)
                     /locus_tag="PC479_000795"
     CDS             complement(33932..34981)
                     /locus_tag="PC479_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2803378.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            35256..36791
                     /locus_tag="PC479_000796"
     CDS             35256..36791
                     /locus_tag="PC479_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803379.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(36976..37662)
                     /locus_tag="PC479_000797"
     CDS             complement(36976..37662)
                     /locus_tag="PC479_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2803380.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            37787..39169
                     /locus_tag="PC479_000798"
     CDS             37787..39169
                     /locus_tag="PC479_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2803381.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            39571..40839
                     /gene="eam"
                     /locus_tag="PC479_000799"
     CDS             39571..40839
                     /gene="eam"
                     /locus_tag="PC479_000799"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2803382.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(40932..41288)
                     /locus_tag="PC479_000800"
     CDS             complement(40932..41288)
                     /locus_tag="PC479_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803383.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(41439..42533)
                     /locus_tag="PC479_000801"
     CDS             complement(41439..42533)
                     /locus_tag="PC479_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2803384.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            42997..43818
                     /locus_tag="PC479_000802"
     CDS             42997..43818
                     /locus_tag="PC479_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2803385.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            43839..45035
                     /locus_tag="PC479_000803"
     CDS             43839..45035
                     /locus_tag="PC479_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2803386.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            45202..48600
                     /locus_tag="PC479_000804"
     CDS             45202..48600
                     /locus_tag="PC479_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2803387.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            48610..50283
                     /locus_tag="PC479_000805"
     CDS             48610..50283
                     /locus_tag="PC479_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2803388.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            50556..50750
                     /locus_tag="PC479_000806"
     CDS             50556..50750
                     /locus_tag="PC479_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2803389.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    50919..51152
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            51229..52626
                     /gene="asnS"
                     /locus_tag="PC479_000807"
     CDS             51229..52626
                     /gene="asnS"
                     /locus_tag="PC479_000807"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2803390.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(52799..53974)
                     /locus_tag="PC479_000808"
     CDS             complement(52799..53974)
                     /locus_tag="PC479_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2803391.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            54393..55757
                     /locus_tag="PC479_000809"
     CDS             54393..55757
                     /locus_tag="PC479_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2803392.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            55779..56270
                     /locus_tag="PC479_000810"
     CDS             55779..56270
                     /locus_tag="PC479_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2803393.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            56543..57214
                     /locus_tag="PC479_000811"
     CDS             56543..57214
                     /locus_tag="PC479_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2803394.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            57299..58165
                     /locus_tag="PC479_000812"
     CDS             57299..58165
                     /locus_tag="PC479_000812"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2803395.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            58198..59700
                     /locus_tag="PC479_000813"
     CDS             58198..59700
                     /locus_tag="PC479_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2803396.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            59830..60282
                     /locus_tag="PC479_000814"
     CDS             59830..60282
                     /locus_tag="PC479_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2803397.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            60442..61245
                     /locus_tag="PC479_000815"
     CDS             60442..61245
                     /locus_tag="PC479_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803398.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            61303..62190
                     /locus_tag="PC479_000816"
     CDS             61303..62190
                     /locus_tag="PC479_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2803399.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            62209..63057
                     /locus_tag="PC479_000817"
     CDS             62209..63057
                     /locus_tag="PC479_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2803400.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            63120..63809
                     /locus_tag="PC479_000818"
     CDS             63120..63809
                     /locus_tag="PC479_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2803401.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT        25369 a         6915 c        11017 g        20559 t
ORIGIN      
        1 taaaatagaa agggttaaaa acacagttta ttataatttt tattgttgag tagatacgta
       61 ctattatata tgattgagca agtttttcaa tcataaattt ataaagtatt ttgcattaaa
      121 atttaaacaa agaattaaat tatttaagat tatgaataac taatacattt agtaaattac
      181 taaatataca aaatgtaaat acttatcaaa atgtataaat acttatgaca tctagttact
      241 tacagggttt aagtagaaaa aaatatcaaa gtaagtatgg catgatagtt gcttatatat
      301 ttatcaagaa aggagttatt aagcatggag ttgagagata aacttgtaaa catcataaat
      361 acagaaaata aaaaagatcc attaacagat gttcaaatag caaagttttt aagtacaact
      421 agagagaaca taactaattt gagaaaagaa cttaatataa gtaattcaag gcaaagaaga
      481 tatccttatt taaaatcagc aatttcggca atattacaaa agaataagaa tatttctata
      541 tcagaaatta caagggaatt gatgacagaa ggctttaata tatcgagaag agttgtggag
      601 gaacttttac ctaaagaaag tttagaaatg gatgtagtag aggaagatga agaagaatcg
      661 aatgacccat ttgaaactct gataggtaat aaagggagtc ttagaaatgc tgtagagcag
      721 gctaaatctg ctatattgta tccaccaaaa gggttaccaa cgttgattat tggggaaagt
      781 ggagttggta agagtttatt ttctagacat atgtatgagt ttgcaaagca aaaaaagata
      841 gtaaaagaga gctccaattt tgttgtattt aactgtgctg attatagtga caatccacaa
      901 ttattattat ctttgttatt tggatataaa aaaggtgcat ttacaggtgc tgaatatgat
      961 acaccaggtc ttgtagaaga agctgatgaa ggagttttat ttttggatga aatacataga
     1021 ttgcctccaa aaggtcaaga aatactattt tctatcttag ataggggaaa gtttaggaga
     1081 ttaggtgaaa ctaatgctga aagaaaagta agtattatgt tgattggggc aactactgaa
     1141 aatgtagaga ctaatttatt attaacattc agaagaagga taccaatgct aataactttg
     1201 ccaccattac atgatagatt actaaaagag aaaatagacc ttatatacaa tatctttcaa
     1261 caagagtgta atagaataaa tgcaaaaata tttgtagata aaaatgtgat tgaaatactt
     1321 gcactaaaaa agtttagtgg aaatatagga caattacaaa atatgattca ggttctttgt
     1381 gcaagagctt ttatgaagtt tataaattcc aaagatgaag atagtgaatc tatagtagta
     1441 gtagatataa atgaagtttt aaagcttaaa gattcgttta aagatgctgc ctttcaagag
     1501 attgaatata cagagataag aaaatatttg aaaaatgcta tatttatacc atttaattta
     1561 gaagaatcat cagtaaaagg aagtctttta agtgatgaat atatattacc agaggacata
     1621 tacaaaacaa ttgaaaagaa atattatgac ctaaaatccc tagacattga agatatagat
     1681 atagagaata ttttatggac ttttatttta aatagatttg ctgacttaaa gtttaatctt
     1741 gatagtaatg aagaaatttt ttcaatgagt gatttaaata gttttgtaag tgatagttta
     1801 gttaaactgg taaaagaact tatgaatgat atagttgaaa aaaatatgaa tttagaagta
     1861 aataaaaata tgttcaaata tctagcaata cacttagaag aagctataaa aagaattaag
     1921 ctaaatcaaa agataataaa tgtaaattta gaaaaaataa agattgactt ttcaaaagaa
     1981 tatgaaatta gtaaaagttt tgcaataaaa attgaggaat caattggtat taagatttca
     2041 gatgatgaaa ttggatttat aacgctctac ataaagtctg ctctaaaaaa tgaagtgaaa
     2101 aaagataaag tagggttaat aataatttct catggacgta tagctacaga gactgtaaat
     2161 gtagttaaag aacttttagg tgtaaagttt cctgtggcta tagatatgcc tttagatgaa
     2221 aaaccaataa atatatacaa taaggcagta gaattgtcaa aaataataga ccaagggaaa
     2281 ggcatattgt ttttggtgga tattggttca cttacaaaca taggtcaaat agtaaataag
     2341 agaaccaata taagtactaa aactattgat agagtagacc tcctaatggc actagaagca
     2401 actagaaaag tttctatggg agaggaagaa cttgatgaaa tatttttttc tttgctaaaa
     2461 gataggatgg gatataacta tgaattagaa aaacacatag ataaatcaaa tgcaattata
     2521 acattatgtc ttactggaga aggaactgct aagtatataa gtaaaacgtt ggaagaaaag
     2581 tatgataaca ctaaatgtta tcaaatgagt gcattggatg aaagtttatt ttgtaaaatt
     2641 gaaaatctaa aagagacaaa caatatatta gctattgttg ggacaataaa ccctaagata
     2701 ccaggtataa attttatacc ctataataaa gaacttttca aaaatttgga tatctatcta
     2761 tcaaaatcaa aacaacaaga tgacagacca attaaatcgt atgaaagaat gcttgatgag
     2821 gatttagtga tttttgaacc agatatatat tttaaaaagg atttattaga atatgtatgt
     2881 tcaattctta taaataaaga atatgtagaa aaagattact tagattctgt tttacataga
     2941 gaagaaatgc ttccaacata ttctaaagga gctataggtg tgcctcatgg agattcttct
     3001 actgtaaata gtacaaggtt tgtttttgtt aaactaaaga atcctattga ttggggtgta
     3061 ggtaatgtaa atttcatatt tatgccagtt tttcaggcta atgataaaga gattgttaaa
     3121 aatgttcttg agatactaaa agatgcagaa tttatgaaca atgccaataa gtgttttgat
     3181 aaagatgctt ttagaaggat tatatttgat aaatttaaac atctgtagcc atagcaaaaa
     3241 aattgagagg agttagagta atgaatgtag atttaatatc attgggttta gaatctaatg
     3301 attatgaaag tgttattgaa gaattaggaa gtataatgtg taaaaaagaa tatgtaaaag
     3361 aaacatacat aaatgctgtc ttggaaagag aaaggacatt accaacaggt ctagatattg
     3421 gagacatgtg tgttgctatt ccacatacag attcaaagca tgtaaatgag tctaatgtag
     3481 ctgtagggat attgaaaaat cctgtaaagt ttaattcaat gatagaccca aaagatagac
     3541 ttgatgtaga acttgtattt ttattagcag taaagaaccc agactcacaa gtaaaactgt
     3601 taaaagattt aatgtcagtt tttcaaaata taaagttatt aaaaaatata aaaaacgcat
     3661 caacaaaaga agaagtagct aaattattgg attttataga aatttaatgt aggagaattt
     3721 tataaataaa aaattaaaat ttaaacaatc aaagtaagta ttattttttt aataggttta
     3781 atcttatata aaataaaaat ttagagtgaa aaattaggag gaaattatta tgaaacaatt
     3841 taatgtatta tcagtttgtg gttcagggac agttacatca tctatggtag caggtaaatt
     3901 aaaagaagta ctaggtaata aggggatttc tattactaca acagaagcta gaccaacaga
     3961 agcactaaat ttagcacaat caggaagatt tgactttata acatttacga gtccacttca
     4021 aacaggcgat tatggaatac caactataaa tgcatttgct tgcctaactg gtatagggga
     4081 agaggcattc tttgaagaag ttttgactgt tattgaaggg atagataagt aagttttgga
     4141 gacgtgaatt tatagtattt aaatcaatga tttagatact ataataagct aatattaact
     4201 aaatatttgt ggaaaaaaca ttttatatgt tgtcataaat taaaaaatta taaaaatagt
     4261 cacttaagta ttaaattaag cttataaata tttataagta ggagggaaaa agatgttaga
     4321 atttatgaaa tctataatcg atacatttgg ttctgcaatt atagttccaa ttataatatt
     4381 cataatcgca aagatattta aagtaactac taagaaatcg tttctttctg ctgtgtatgc
     4441 aggagttgct ttacaaggtt ttacactgat tttaaattca tttacaccag taataacacc
     4501 agttatcaat aggatggttg agagtacagg agtaaaccta ccagtttttg atgttggttg
     4561 gcaagcaaca tcattggttg cattttcaac aagtgcaggt atgatatatt taggtcttgg
     4621 tatattatta caaacaattt tatttttgat aaaatggaca gatgtgtttc aaccatcaga
     4681 tttatggaat aactattctt atatggtatg gggagcaatg gttataggag taacaggaaa
     4741 ttttccttta ggtatagcat gtatggtttt attaaaccta tatagtttat taatatcaga
     4801 gttagttgca aagagatggt ctagttacta tagatatcct aattgtacaa taatagctat
     4861 gcataatgtt gaggcttctg tgtttgcagt atttgcagac cctatataca ataaattggg
     4921 attaaataaa ataaaattaa atccaaaaga attagagaag aaattagggt ttttaggtga
     4981 accaattact ttaggtcttt ttttaggtat gtttataggt atattaggta atatgacgag
     5041 aataaatact atggaagctt ggggagaaat aatgaaagta ggtatatcta caagtgcagt
     5101 gatggcaatt ttccctaaag tagcaagtat gtttgctcaa gcatttgcac ctataacaga
     5161 agcagctaga aagattatgc aaaaagctgg aaaccgtgag tggtacatag cagtaaatga
     5221 tgctgttgga tatggtgaac ctgcaacttt aatatcaggt cttatattaa ttccaataat
     5281 gcttgtaata gctatggtac ttcctggtaa taaggtatta ccagttgtgg atttattggc
     5341 aattccatat atggttcaag gtttagttgc catacataat ggtaatatac caaaagtatt
     5401 agtttcaggt ataatttggt ttggattagg attatatgtt tgtacatcta cagcaccatt
     5461 atttacagat atggctacaa atataggtgt agctatacct gcaggagcaa tgttaataac
     5521 aagttttaat atactaggaa aacctttgat gggattagtt ttctttgcat ttctaagtgc
     5581 aaatccaatt tatataggtc tttcagttgt aatttacttt gttctatggg cattgtttag
     5641 aaagaacaaa acaagtattc ttgattattt agaaaaacaa gcacttaaaa atgtagaaga
     5701 agagcctgta gcagtttaag tttataaaaa tgaaataaaa ataattaaag gagtttataa
     5761 ttgattttat ataagtatca gataatacct gagaaatatt taaatgaaat atagacttct
     5821 ttttatttga taatatttgt aattataatg cattgtattt ttatagttga gttttctgta
     5881 taacctgcta tttaaaacat actcttttat atttatacag taatgtggtt taattttata
     5941 aaatgtaatc tctactggta aaagactttg tatataaggc atttgcctga ataaaccatt
     6001 tatcttttgt atggcataga tattgctata tagaagtata tagcatgaaa ggagatattt
     6061 atatgggaaa taaaatgaga gcatcagtat tgtataatgt aggtgatgtc agatatgaaa
     6121 tggtaaatat accagaaata acagatacgc aagtgcttgt aaatgtaaaa tatgttggga
     6181 tatgtggttc agatttacct aggtctatgg taagtggttt gagtggaaat acaaaatatc
     6241 cattgatttt agggcatgaa ttttctggtg aagttgttaa aattggagaa aaagtaaagc
     6301 atattaatgt aggagataga gtagctgttg caccattagt accttgtgga aaatgtgatt
     6361 attgtaatga aggtaatttt ggcttgtgtg atgattacaa tataattgga acaagagtaa
     6421 atggagcgtt tgcagaatat gtaagagttc cagaagaaca tattttaaaa ctaccagata
     6481 cattggatta tgaaactgct gccggaattg aaccagcaac tatagcttat catgggattt
     6541 caaagtctaa tattagagta ggagatagtg tagttgtgtt aggatgtggt ccaataggtc
     6601 agtttgttat acaatgggca aaggtgtttg gtgcatctaa gattatagca gttgatattt
     6661 ttgatgaaaa attagaactt tctaaacttc taggagcaaa ctatatactt aactcaaaag
     6721 aagtaaatgt aatcaaagaa ataaaaaaaa taacaaatgg tggtgctgat gttgtaatag
     6781 aaactgctgg tagccgattt acacaagaac aatctctatt tgtagcaaaa aagagaggaa
     6841 atatagtgtt tgtaggtata tcacacacag aattaccact tagtgcagac gctactgagt
     6901 gtatattacg tggagaactt accttgaaag gttcttggaa ttcatataca agcccttatc
     6961 ctggaagagc atggactgca acattggatt ttatggaaaa aggtgacatt atttttaaac
     7021 ccatgatttc agataaaata ggtcttaatg aagtgggtga ctttttaagt aaaatgagca
     7081 agagagaaat taatttcaat aaaatcctag tagaaatata aaaatttaat aaaaaattct
     7141 aaaagcaatt aaattttata actatgacat taaataaaat aaaaataatt ttcttaaata
     7201 aaatatagga ggaagaaaag tgttagaaaa tttaaaagaa gaagtattaa aagcaaactt
     7261 agaattacct aagaaaaagc taattactta tacttgggga aatgtaagtg ggatagatag
     7321 agaaaaagga ttagtggtga taaagccaag tggtgtagag tatagtaata tgactgtaga
     7381 agatatggtt gtagttgatt tagagggaaa tatagttgat ggcaaattaa gaccttcttc
     7441 agataccccg actcatctag ttttatataa agagtttgaa gaattggggg gaatagtaca
     7501 tacacattca agttgggcaa caatttgggc tcaaatagga aaatctatac catcttgtgg
     7561 tactactcat gcagattatt tttatggaag tataccatgt accagaaaaa tgactagaga
     7621 agaaatttgc aatgaatacg aaaaggaaac tggaaatgta atcattgaag aatttatggg
     7681 aagaaatcca attcattgtc caggaataat tgtaaatgac cacggacctt ttacatgggg
     7741 aaaagatgca aatgaggctg tacacaatgc agtagtttta gaagaagtcg caaagatggc
     7801 ttattataca gagttaatga gtcctgataa tattatggat aaggttctta tgaataagca
     7861 tttttcaaga aaacatggaa aaaatgcata ctatggtcaa aaatgattat ctggaggtga
     7921 attatgctag actctttaaa aggtaaagtt gtggaaatag caattaaagc agagcattat
     7981 ggtttatgta aagaaaagac tggtaatttt agtattagag ataaggatac tggatatata
     8041 cttataaccc catcaggtgt aggaagagaa gaattaaaag ttgaacatat ttgtgtattg
     8101 gatttggatg gaaatgtaat agaagttgag aaaggtatta agccaagtag tgaggtattg
     8161 atgcatattg aaatatacaa aacaagagaa aatataaatg ccatatcaca tactcattct
     8221 ttatatgcaa cagcattttc agtagcaaag aaaccaatta taccaattgt atatgaatca
     8281 gttaattatg gcggttatgt agatattgct tcatacgaaa gaccaggaac tttaaaatta
     8341 gctaagagtg tttcaaaatt actgatagat acagatgcat gcttattaga aagacatggg
     8401 ttgacaactg taggtaatga tttagacgaa gcattattaa aatcaagata tgtggaagag
     8461 gtagcagaaa tatactatag gtctttagta ttaaatcaat ttaaagaact tgaagtagta
     8521 gatgttgatg agtttaaatc ttggaaatac ccagaagaaa taaagtttta atctaaataa
     8581 ttttcattga agtggttaat taaaatatat gtattattta tatcaattac tatgttttat
     8641 tatgttaatt accgtatttt gcaaatatat ttattacatt ttttcaaaaa aatatagaat
     8701 ccgaaagcca tataaaattg acttgggatt ctatactttt tatttatgca tttttcttta
     8761 tctgattttc tggagaattt atatgttcaa aatatttttt agtttcagat actattatag
     8821 gtgataatgc aagtaaagct ataaggttag gtattgccat aagcccattt acaatatcag
     8881 caattatcca aacaacttct aactttaaga aagcacctaa taacaccata gccacaaata
     8941 cagttctata cccattcatg ccttttacac caaacagaaa ttcaaaacat ctttctccat
     9001 aataactcca tcccaaaatt gtagtaaatg caaataatgt taaactaact gtaagtaata
     9061 ttggtccaaa tgtaggaatt gctccgttaa aagctgcttg tgtcataaca gctccattta
     9121 ggtctgaaca ccacactcct gaaataacaa gagagaatcc agtaagagta cagattataa
     9181 tagtatctat aaaagtacct gtcatagata taagaccttg ttccgctggc cattcagttt
     9241 tagcagcagc tgcagctatt ggagcactac ctagaccaga ctcatttgaa aatacaccac
     9301 gagcgatacc atttctaatt gccagtgaca tagttgctcc taaaaaacca ccagtagcag
     9361 cagtaggtgt aaatgcactt ttaattatta acataaaagt ttcagggatt ttattccaaa
     9421 aagatattaa aatgattcca cagattacaa catatataac tgccataaaa ggtactattt
     9481 tagtagctac attagatata cttttaagtc ctccaaatac aactaaagct acaaatactg
     9541 ctaatacaac ccctgtaatt ctagggtcta tcccgaaact attatttatt gcatctgtta
     9601 ttgaatttac ttgagtaaaa gtaccgattc ctaaaagtgc aactagtata ccacttatag
     9661 cgaaaaatat tgcaagaggt ctccattttt ctcccatacc atttactatg taatacattg
     9721 gcccaccaga cacttgacca tttttatctt tagttctgta ttttattgca agaacacctt
     9781 ctgaatactt agtagccatt ccaaagaatg ctgctatcca catccaaaaa agtgcaccag
     9841 gtccaccagc tttgatagct gtagcaacac caacaatatt accagtacca actgtagcag
     9901 ctagagcagt acataaggct gcaaaacttg aaacatcacc actacctttg ttttcagcag
     9961 agaatattaa cttaagagct cttggcagtt tagttatttg aacaactcca agcttaacag
    10021 taagtagtat accagtacca actaatagta ccagtagggg tggtccccaa ataaaagcat
    10081 caactttgtt taaaatatca attagattca taaaaacact ccctcatttt ttttgcttta
    10141 cataatcaat agaattttta atgaagtaat tgacaggaaa taagtataat ttattaataa
    10201 tttctagtgt aataaaaaaa gctaaatcta aaaaagattc agctctggat gtacactaat
    10261 tagcaaccaa aataaaagta ttagtaaata ctgtttttgg tagataatcc ctgtcctttt
    10321 gcctgagaga ttgagcataa aagccttgcc ccttcggcgc ccataatggg tctctccaga
    10381 gttccgtaaa ttttacagtc ctacttttaa aagtacctga gagtgttact ccttcggtgt
    10441 gctatatagc aactcttcta taaaatctta accggatatt gatttgtcat agcttattgt
    10501 attaataata ttaatataag tttttatgtt ttgtcaacta aaaaattaaa aaacaaagaa
    10561 aaaatataaa aatgtaagcc tatttacgaa ttataaactt agaaagatga gtaatatacg
    10621 tgaaaatgaa ttttgtaata cacatacaaa tgaagtgtaa aataagtttt aaataaaaaa
    10681 ataataaaat cttaaataat taattagata tgattaaatt atggtaatat tatagttacg
    10741 atattaagaa aagttctatt tagttagatt ttaagcgtaa tatgagatta tgtatagaga
    10801 aaggatgtat ttttagatgg gaaaaatatt agtgctagca gaaaaacctt ctgttggaag
    10861 agatatagca aaagttttag ggtcaaaaaa tgaaaaaaat ggttatatag aggggccaaa
    10921 gtatgtgata acttgggcac taggtcattt ggttacactt gctgaccccg aaagttatgg
    10981 agaaagatat aaatcatgga gcttagaaga cctgccaata cttcccaagc accttaagac
    11041 tgttgtaata aaaaagagtg gaaagcagtt taatacagtt aaatcgcaaa tgaatagaaa
    11101 tgatatagat gaaatagtta tagcaacaga tgcaggtcgt gaaggagaac ttgtagcaag
    11161 gtggataata gaaaaatcac aggtaaaaaa acctataaaa cgtctttgga tttcttcaag
    11221 tacagacaaa gcaataaaag acggatttgc aaaattaaga agtggaaaag aatatgaaaa
    11281 tttgtattac tcagcaatag cacgagcaga agctgattgg attattggaa taaatgcaac
    11341 cagagctttg acaacaaagt acaatgcaca gctttcttgt ggaagagtac aaactcctac
    11401 tcttgcaatg ttgcttaaaa gagaagaaga aataagaaat ttcaaaccta aagagtatta
    11461 tggattagaa ttaattgcta caaagggaaa ttcagatata aaatttattt ggaatgataa
    11521 aaacaataat tcttcaactt ttagtaaaga aaagatagaa tccacattaa aaaaagtaaa
    11581 aggtgtagac ataaaaatat ctgaagtaaa caagtcatat aagaaaaaat attcacctgc
    11641 actctatgat ttaacagaat tacaaagaga tgcaaataaa atgtttggat tttcagcaaa
    11701 agaaacttta tctataatgc aaaaactgta tgaacatcat aaagtactta cttatcctag
    11761 aactgattct agatatttaa catcagatat tgttggaaca ttaaaagata gaataaaagc
    11821 tgtaaatatt agtgattatt caaagatttg taatagatta ttaaaaagca atattatagc
    11881 taataaatcg tttgttgaca attcaaaggt aacagaccac catgcaataa taccaacaga
    11941 acagaaggta ttcatgagtg attttagtga taaagagcga aaaatatata atcttgtagt
    12001 aaaaagattt ttagcagtac tatctcctcc ttttgaatat gagcagacaa ctataagtgc
    12061 aactgtagaa ggagaaatat ttaaggctaa gggaaacaaa gtaataaatt taggttggaa
    12121 agaaagttat aaagatattg aagatgaaga tgagtatgaa agtataccaa atgttttaga
    12181 aaaagatgta cttaaggtca agggattaaa gataacttca ggaaaaacaa atccaccacc
    12241 atacttaaat gagggaacat tattgactgc tatggaaaag aatggtctag ggacagttgc
    12301 tacaagagca gatataatag aaaaattatt caattcattt ttaatggaaa agaaaaataa
    12361 agatatatat ataacatcaa aaggaagaca attattagat ttagttccag tagacttaaa
    12421 gacaccagaa cttacagctt catgggaaaa gaaattattg gatatttcaa agggaaaact
    12481 tagtaaaaat acattcataa atgacatgaa agattattca aaagatattg tactagaagt
    12541 aaaaaatagt gataataaat ttaaacatga taacttaact aaaaatagat gtccagaatg
    12601 tggtaaattt atgttagagg taaatggtaa aaaaggtaag atgttgattt gtgaagatag
    12661 agagtgtaat actagaaaaa ctatatcaca agtaaccaat tcaagatgtc ctaactgcca
    12721 taaaaaatta gaacttcgtg gagaaggtga ggggagactc ttcacttgta gttgcggata
    12781 cagagaaaag ttgtcttctt tcaacaagag aaagcaagaa gaaaaacaga aggcatcaaa
    12841 aaaagatgtt aatcaatatt taaaaaatca aaataaaaat caagagacat ttaataatcc
    12901 atttgcagaa gcattagcta agctaaaaaa caaataattt taaagttatt attatacaca
    12961 attataactt taactgtata ttagttatat gtactgatat aatatagtgt atagtttagg
    13021 catgaaagta gggctgttaa atggatttaa gtggcatatt taaatactat tgtaaagagt
    13081 gtgaaaatac atggaataat tcgagtgttg aattatttga gaatatagaa acgtatagta
    13141 aagattcaca aaaaaagagg gaaaaagaat tagataaatt gctaaataca atatcagttc
    13201 atttagagag gtatccaagt gatgctgtat tgagaaaaat gtgggtaaaa aagggcgagg
    13261 ttttcttaca aaagacattg gaaaaagaaa atatttttaa gttagaaaaa atggatgtag
    13321 aggatagaaa aaaattttta gatataacaa aacagtttat tagagatgct agaaaatttg
    13381 atgatgattt acctataggt gatattatgc aagctatgag gaatgtatgg atttcaaatg
    13441 cattacaatt attatttggt aaagaagtat attattcaaa agctaacttt gcatatagta
    13501 tgttatatcc atatactgat aattatttag acaatacaaa tatagataaa aatgataaga
    13561 ttttatttaa taactggtta gaaaaaaggc tcctgggaga acacattaaa tctaaggatt
    13621 atcatgaaag taaagtatct caaatgatag attatattga aagtgtatac cctagagaaa
    13681 agtttacaga agtttatgaa tcgttattat taatatttaa aagtcaagta aatagtttaa
    13741 aacaacatgg taaggaaaat catttgtgta aagaagattt attatccatt tctattgaaa
    13801 aaggaggttc atccgtttta gtagatggat atttaataag tggattgatg acaaaggaag
    13861 aaatagagtt ttgtatagga tatggatttt tattacaaat atctgatgat ttacaagata
    13921 taaaagaaga tttaaaatac aaccataaaa ctattattac agagatgtca aaagagggta
    13981 ctttagataa agttgtaaat aaactaataa attttactat tgagttaata gatagtttta
    14041 aaattaataa taaaaataaa tctgtaatag ctatgataaa gaatgattgc ttaatgttaa
    14101 ttttattttc tgtagtttat aatgctgaat ttttttctgt aggatatata aaagaagtag
    14161 agaaatttat tccatataca atagattatt cattagagat tgaagaaaaa ataaaagaaa
    14221 aatttaaaaa tatagatgtt ttaaataatg aaaatgaata taaagaaatg attgatatta
    14281 tttgtgcaga gtagaaaaaa agggagtgtc ttagaataga ctttttagtt tatgaggcac
    14341 tctttttgta taaaatcaaa tgtattttca tcattttaac aattaaaaag agcttatccc
    14401 tatttgagaa agcccttttt aatttaaata aaaatgtcaa ggttaaacta tttaaatgca
    14461 gtaataatcc cattatcatg taagtttttt atgattgtag ctgcgatagg gaatccaaat
    14521 aatccagcta ttccaaatag ttgagctcca acaaacatac acattaatgt tattaaagga
    14581 tataggccaa tttggtgtcc tactaccttt ggttctaata tttgtctaac aacggttata
    14641 attatgtata gtattaaaag tcctattgct aaattaatat ttccattaat gagtgagata
    14701 atcatccaag gtgtcaaaat acttcctgta cccaagactg gcaaaatatc aacaagtgct
    14761 atgatgacag atatagtaaa tgcattttca acctttagga tagtaagtcc aataagtagt
    14821 tctatgaatg tcactgatag tagtattgca taggctttta gtagttttaa aagagcatta
    14881 atactgctat tttttatatt tattatcata aattgacttt tttctggaaa ttgttttaag
    14941 ataaagttta ctattttttg atagtcaaaa gtaaagaaaa aagaagcaat taaagtaata
    15001 ataagtttta acataaatgc aggtgtttta cttgcaatac tagcaatagc attgagtgca
    15061 tttgttgaaa tagaagctac taaagacatc atagattgac ttatattatc ccaaccaagt
    15121 attaatgcta ggttaacatt aggtgtacta gcatctatct ttgaaaataa agtatttagc
    15181 gttggttgta tacttgattt atatatttca ggaagtctaa aaaatacatc acttacactt
    15241 gcaaatatct tcacaccaaa gctaactaat aaaaatatac acaacccata aaaaattaaa
    15301 agtattacta tagaaataag tgtacgattt aaatttgtat tatttgtaat aagtcttata
    15361 ataggtctta agatggatgc aatgataaaa ctgattacaa aaggcattaa taatggcaat
    15421 aagaatttta atacaatata tacaatactt gttattaaaa ctacatatgt aaatttaatt
    15481 ataaaattct tttgcttttg aatatccata atataaaacc tccaatgtat ttgatattat
    15541 tctaaatatg gtctttttta cagaaaatat actactgtat attgattata aactataaag
    15601 cttatgtaaa gtaaataaat tatgtaaaat ttaggttaat tttgtataaa gagatacttt
    15661 tatattttac aattaataac caaatactaa aaaatatttt aaataaagaa cttaaaattg
    15721 tgataaaatt aatttataga tattaaaaaa agtataatat atggttttta agaggtgcta
    15781 agatgaaaaa ttttagagat ttaggtggga acaaaactga ggatggcaga actgtaaaga
    15841 agggattatt ttataggtct gcaaaattat caaatctaag tgaaaatgat ataaagattt
    15901 taaaagagtt aaatattaaa tatatatttg attatagaag tgatgaagaa gctcgtaaac
    15961 atccatcaac aataatatca aatataaaaa atataagaat accagcaatg agagagctag
    16021 aagaatcagg aggaagtttt ggttctattg aagatatgat tgatggattg tttgaaaaag
    16081 atggtgcatt taatatgtta aataatagtt actataattt gcctataaat aatccgtcat
    16141 ataaaaaatt agtagaactt ataagagact attctaattt accaatatta aatcattgta
    16201 cagctggtaa ggatagaact ggtgtgggga gtgctataat tcttatgatt ttaggggtat
    16261 cgagagagaa tataatgaaa gattatctaa aaagtaatga ttttgcagat aaagagatag
    16321 aaagatttat agaatataag cctaaattta aggacattcc aaaagaaaat ttgaaatata
    16381 tatttggtgt aaatgaagag tatatgaaaa ctgcatttag aagaattgat gaagaataca
    16441 ttagtgtaga agcatattta tatggagaat ttaatttaaa taaagaagaa ataagaaaat
    16501 taagaaatca atatctagag taaaaggtga gtttatgagt ttaaatataa cagcaagaca
    16561 gataaatata ataaaaatat tgttaaattc aaaagataca attagtggta ttgccttaag
    16621 tcaagagata ggatgttctt caaaaactat acaaaatgaa ataaaggata ttaataaaca
    16681 acttagaaat ggtaaaattt tgtcaataag aggagttggt tataaactag aaggaagttt
    16741 tgatgaggta aatctaaata gcaatctcta tgatgatgta gatagagtag aatatataat
    16801 cagaaaaata ttaaccttaa gtaatgaaga aaaaaatact ataaaacttg aagatttagc
    16861 cgattctatg tatgtaagtg tttcaacagt aaaaaatgat ttaaaagaag taaaaagaat
    16921 gttggaaaag tattcaatat cagttaaatc taagcataaa caaggtatat gtgttttgga
    16981 aagtgaagat aagatattaa attgtatagt tgacttaagc aataagagag ataatcaatt
    17041 gagtttaaat gattttttaa atgatgatat aaaggaaaga aaactattaa taaaaaaatt
    17101 attattagac atattaaatg aaaatggatt agtattaact gatatagaat ttaagagtgt
    17161 gttaaataat atattagtta tgttaagcag acataagtat aatgaaaaag aatttataga
    17221 gaattatata gaaaattata ggagcaaaag aagctcaatt ttagaaaatg aacaaaacaa
    17281 agaaattata ataaattcaa taaaaacatt ttgtaaaaat ttaaaattag ctacatcaat
    17341 agatataagt aatgataagg tatttgaaga gtttttgtat aagcatatta gtagtttatg
    17401 taaaaaaata aagttaggta taaatcaatc acctataatg gctcaagata taaaaataaa
    17461 atatccattt gcttttgaac tagctaatat agctaaaaag acaatagaaa aagatttgaa
    17521 gatagaaatt aatgaagatg aagttgcaaa catagctcta catataggag gagcagttga
    17581 aagagcatct tataataata aagataaggt tttcaaaact ataatagttt gtacttcagg
    17641 cataggcaca tcaatgttaa taaaagctaa attagagaat atatttaagg aaaaattaga
    17701 aatactaaag attattcctt catacttagt tgaatatata aaagttatag atgttgattt
    17761 tgtaatatct actgttccta ttgaagtagg agatgtgcca gtaataaata tatctccaat
    17821 tttaagtgag aaagaaatta gattaattga aaaatatata gaaacaggaa atatatacct
    17881 agatttagat ataaaaagcc tgtttgatag tgaattgttt tttactgatt tggtgtttaa
    17941 aaggaaagaa gaagttatag attttatggc aagtaaactt gtacaaaagg gctatataga
    18001 tgaggatatg aaaaaatctt actttgagag agaaaaaata gcaacaacag aaatagggaa
    18061 tatggtaact ataccacatg gagctattgg aaaaattttt gataataaaa tagcaattgg
    18121 tatattaaag aagcctattt cttgggaaat atcagatgtt cgtttaataa taatgttagc
    18181 ccttgataaa gataaaatat tagattatga agtaatattt tcaaaaatat ataaaagagt
    18241 agattctata gcaaaagtta taagtatatg tgaaaatagt agttttgaaa aatttgtaaa
    18301 tttattcaat tagatgggta gtattgtata tacacctgta aatttggata atataaaact
    18361 aaataagata aaaacgataa atttctcttt taattaagag aaattttttg actttcaagg
    18421 atattaatat aaagttaaga tataaacata aagcaaataa ataaaaaaga ggtggcaata
    18481 tgatttatac agtgacatta aatccatcaa tagattatat agttaagcta gatgagctaa
    18541 agacaggaag tacaaatcgt gtaaaagaag aatacgttta tccaggagga aaaggtataa
    18601 atgtatctcg catatttaag gaacttggaa atgataatac atctttaggt tttataagtg
    18661 gttttactgg agaatatata ataagaacat tggaagaaaa agaattgaag acagatttta
    18721 taaagataaa aagtggtttt tcaagaataa atgtaaaaat aaaggcgtta aaggaaactg
    18781 agattaatgg acaaggtcca aatatagatg atgaagacat tgatacttta tataaaaaac
    18841 ttgataagct aaatcaagat gatattttaa tattggcagg aagtatacca tctactttag
    18901 atgaaaaact atacgaaaat ataatggcta gattagaaaa aaagaatata aaagttgtag
    18961 tagatgcaac taaaaatctt cttcttaatg ttttaaaata taaacctttt ctaattaagc
    19021 ctaataacga cgaattagaa gaactatttg gtgttaaatt aaattcaatt gaagacatgg
    19081 taaaatatgc tagaaggtta aaggaaatgg gagctataaa tgtcttggta tcaatgggaa
    19141 aagatggagc attgttgatt acagaggaag atgaagtgct tataagtgat gttccaaaag
    19201 gaaaagtaaa aaattctgta ggagcaggtg attcaatggt agcaggattt atatctgggt
    19261 atttgaatac tgggaaatat gattatgctc ttaaattggg agcagcttca ggtagtgcaa
    19321 ctgctttttc atatgactta gccaaaagag aatacattga taaattggta aatgagatat
    19381 ctgtaaagca gttttaaaat attaaaaaat atatagaaag gtaattaata tttatgaaaa
    19441 ttatagattt attagatgaa aagtcaatta agttaaattt aaattcaaaa accaaatcag
    19501 aagcaataga agaattagta gatttggtcg caaatagtgg aaatttaaat gataaagaaa
    19561 actacaaaaa agcaattttg gctcgtgaag aaatgagtac aacaggaatt ggagaaggtg
    19621 tagctatacc tcatgctaaa aattcttcag ttactaaagc ttgtatagca gcagcagtgt
    19681 caaaagaagg tatagattat gaatcgtttg acggaagttt atcaaatcta ttttttatga
    19741 tagctgcacc agacggagct aacaatactc atcttgaagt attatcaaga ttatctacaa
    19801 tacttatgga tgaagacttt agaaataaac ttataaattc aagctctgaa aaagaattta
    19861 aggaaataat agataaaaag gaaagagaaa aattcagtga agaatatcaa gatgagaaag
    19921 ttactgaaaa aaatataata gatactaaag agaatgatgc taataagtat cctaaggttt
    19981 tagcagtaac agcatgtcca actggaatag cacatacatt tatggcagct gaaagtttaa
    20041 ataaaatggc agaaaacaaa ggtgtatcaa taaaagtaga aactaatggt tcagcaggtg
    20101 ttaaaaataa gcttacaaaa gatgaaatag aaaatgcaac atgtataata gtggcggcag
    20161 ataaaaatgt tgagatggct agatttaatg gtaaaaaagt cataaaaact aaggtggctg
    20221 atggtataca caaagcggaa gaacttatag acaaggctgt aaatggagat gctccaattt
    20281 accatggagg agatggaagt cacaacgaat caaatgaaga aagtgaaagt ggatttagaa
    20341 aagtatacaa acacttaatg aatggtgtat ctaatatgtt accatttgtt attggtggag
    20401 ggatacttat agcgatagcc tttttacttg atgattatac tattaaccct tcaaactttg
    20461 gttcaaatac acctatagca gcatttttta aaggaatagg agataaagct tttggattta
    20521 tgttacctgt acttgctgga tatatagcat actctatatc tgatagacca gcgtttgttg
    20581 taggatttgt aggaggtgct ctagcaggtg atggtggttc aggtttttta ggtgcactac
    20641 ttgcaggttt tatagcaggt tatttagtag aagggcttaa gaaaatattt agtgtactac
    20701 cagcttcatt ggaaggaata aaaccagtat tgttataccc tttacttgga acacttttga
    20761 tgggaattat aatgacattt ttagtaatac ctccagttac agcaattaat aatgcaatgg
    20821 ttggattttt aaatggttta ggtggaactt caaaaatagt tttaggatta gtattaggtg
    20881 gaatgatggc tgtggatatg ggtggacctg taaataaagc agcatatgta tttggagttg
    20941 catcacttga atcagggcag tttgaaataa tggcagcagt aatggctggt ggtatggttc
    21001 cgccacttgc tatagctctt gcaactacat tttttaagaa tagatttact aaagaagaaa
    21061 gagattctgg taaagttaat tatgtaatgg gattatcatt tgttactgaa ggagctatac
    21121 cttttgcagc tggagaccca cttcatgtta taccagcttg tgttggaggt tcagcagtgg
    21181 caggagcact tagtatgttg ttcaacgcag ctcttcgtgc acctcatgga ggagtatttg
    21241 ttataccagt ggttacccat ccatttgcat atatattagc tatagctgta ggtgctttgg
    21301 ttggaatgat gcttttagca ttacttaaaa aaccattaaa tcaagaagta tagcaattaa
    21361 aggatattca agaacttaaa atatgatgtt ttataaaaat taatatattg ctgaattatt
    21421 ttaaaataag gattttatga ggctgttata taacgatttg taattgttat cttgacagtc
    21481 ttttattttt atataagtaa aataagtgtg ttttgcttta tttgtattta aattgttttt
    21541 aagttagaat ataaagaaaa ttatatatta ttttaatatt cactcgaact agaaaataat
    21601 atttgttgta tttttttgta tataaaatat attgtaatat taaaaaaata atagtaaact
    21661 gattgcataa aaatgatttt tatataaaat ttaagcagaa gaataaattt ctaacgaaat
    21721 tggatatttc ttctgtaaat acatattcaa aaaaagaatt ataagagttt agtcagcagt
    21781 cgtgtaagta ataaggactt tataaatata tagaaatata atgatattaa tataaccaag
    21841 cgatttttaa tttagctttt taataagctt attttttata ctatttataa taaataaact
    21901 ccatgatggg aaaactatta ctatttaata tgtaaaggag tattttaaat gaatgaatca
    21961 tattggtttt taaactcaag tccaaaagaa tataacaaat taggtgaaaa tataaaaaca
    22021 gattgtctta tagtaggtgg tggaataacg ggattgacaa ctgcatattt attagcaaaa
    22081 gaaggtaaaa aagtagtgct tgttgaggct gataaaatag gatatggaac ttctggaagg
    22141 aatactggta aggtaacttg tcagcatgat atattttact ctaagataga gaaaaaatat
    22201 ggattagata aagcaaaatc atattataat gcaaataatg aagctttaaa tttagttgaa
    22261 caaataatag aagaaaacaa tataaaatgt gattttaaaa gggaaacttc ttttgtattt
    22321 acagaaaaag aggatactat aaaggatatt aaagatgaat atcgtacatg taaaaaaata
    22381 ggaataaatt gtgagtatca tgaaactata gaaaatattc cattagatat aaagggggct
    22441 attagtttta ctaatcaagg tcaatttaac ccaaagaagt atattgatgg attggcaaaa
    22501 gcagcagtaa atttagggct aaaaatatat gaaaatacac cagtagtaga ccttgaaaaa
    22561 ggaaaaatat gtagggttaa aacaagagag gataatataa tagaagctga gaatgttata
    22621 atttcatctc atagtccatg gtatgatgga ttgaattttt attttgcaaa agaatatgca
    22681 gagcgtgctt atttaatggt tgcagttttg gaaaataaat tagcagatgg tatgtttata
    22741 agtatagatg acccatcaat aacttttaga caatataatg atggaagtga aaatttactg
    22801 atttttggag gaggagacca taaagttggt cagggaggaa ctgagaaaga aatatttgat
    22861 gatttagagc attatggtaa agaagtcttt aaggtaaaag attttaaggg gaaatggtct
    22921 gctcaagata atatgagttt tgacaatgtt ccttatattg gttatatcaa caaaagagaa
    22981 gacaatattt atgtagctac agggttctct aaatggggaa ttactaatgg aactgctgct
    23041 ggaataataa taaaagattt aataataaat aataattctg attataaaga tacttttaat
    23101 ccatccagac taggtagcta cttttcaaaa gattttataa aggaaaatgc aaacgtagca
    23161 ataaattatg taagtggaaa attaaaaata ggtagtggag atatgcctaa aaataatgga
    23221 gaaggaaaga ttgttaatat tgatggaaaa agatatggtg tatataagga tgataatgga
    23281 gatttttata ttgtagacac tacatgtact catttaggat gtgaacttaa ttttaacagt
    23341 gaagaaaaaa catgggactg tccatgtcat ggttctagat ttgactataa gggaaatata
    23401 cttgaaggac cagctcttaa gccattaaaa ctttatggac atggtgataa tgatgttaat
    23461 cctaagttat tataaaatta tttttaggaa ggtaaagtgt atatgaataa acttttaaag
    23521 ccaataacta tactattgta tatagtttat attttatctg ccgtattttt tgtagcaaac
    23581 aaagaatatg gaagtctagg tctagtagca attagtttag ttggtacttt cattttgagt
    23641 tttataaata agcaaactga aagattattg agtaaagaat tatatataat gcttgcttta
    23701 tttataatgt tttcatcatt gttaggtact tgttttaatt tttatgatat aaattattat
    23761 gatgattttt tgcatttatg gtcaggtata atatcttgta ttgtggcttt ttctataatt
    23821 aaatactttt atacacaaag agagataagt aaaatgagta aagtgttttt agttatattt
    23881 ttatttatgt ttagtatggg agtagcgagt ttatgggaaa taggtgaatt tttaatggat
    23941 acttttgttg ggactactac acaggccgga gggttagaag atactatgat tgatatggta
    24001 gatgctttaa taggcacaat tataactatt ccttttatag taaaaaaatc taatatatag
    24061 ttttaaattt tttatattaa ttataatgga tttaattaat catgttgagt ttcaaggtct
    24121 atatgattaa ttaaatccat ttttgttatt tataattatt attgaatata tttttacata
    24181 taagatataa ggtgatagat agaaattgtt ggattatgta aataattaat atataatacc
    24241 attatgggca ataaatacaa atcaaatgat aatatagtct atttatgtaa atgttatatt
    24301 gtatcctaag tataatagta agttttttga tagtggtgta gaaagtaaaa atctactagc
    24361 ctaaattgat gtatggatta gtttaggaac agtaattgca tatcttaagt ttgatgtcat
    24421 gttgggctac tagaaatagg tcatcttttt cacttgaata tcttttcttt aaacatgggg
    24481 agtatcatat caaaaacata tgaaaattaa gtgatttatg agaacagtaa tattttatat
    24541 gatagtatag aatagtaata aagtattagg aggaacattg agtgttaaaa aagtggtttg
    24601 gtattgtgaa aaaaagacaa aaaagtgagt cagtaaaaga agaaggtgaa gtaatattaa
    24661 aaaacgaaaa aatattatct gaagaaaagt tgatagatga agaaggagtt gtagttaata
    24721 ttgataatca aatattaact aaagtagtaa atgatgacaa tgaaatagaa gaaaaaataa
    24781 tagaagaaga tttgttaatt agtgaaaata ccataaaatt agatgataaa gaagcaatta
    24841 ttaatgataa aaacatagaa ttatgtaaaa aagaagttca agttgaaagt gaaaaaatag
    24901 gtttaaataa gtttgaagga ctggaccaaa atgaaaatta taatctagaa aaaaatgtta
    24961 ttgaagaaaa aaaagtaagt gaatgtttga cagaagaaga tttagagtac ataaaagaaa
    25021 ttaaaataaa aagaggaaaa agtataaaag ctataaactt gtatactaaa gaagagtggg
    25081 tttttgacac tcatatacag tgtagtaaaa aactcaaagt tccattaggg tacataagag
    25141 aaaatttaaa atatggatat atggattact ttggagatgc aataaattat ttaagtgaag
    25201 tattaaatat agatgaatac tgcaaaagtg aatggagcta tctcgataat agcaaatctc
    25261 catctgagat atttaatatt ctaaacaata aaatatttag tataaggctt tctaatgaaa
    25321 aaagaaatga aattttgaca aatgataaaa ttgaagcatt aaaaatgaat tatagatttg
    25381 aatgtattga tgaagaatat gatgaatatt ttaaaaaata taagtctata atcaaaagag
    25441 gtggaaagaa aaaagttgaa ttagtaaata aaaaaggtga cattttagaa atatttaagt
    25501 ctttagaaga atgtgccatt tatttgcaaa aggaaaagaa tgaagttata caaatgttaa
    25561 aatatggaga tacaaaagta ggaagaaact ttataaggta tagtttgaga agtatttaat
    25621 ttcattttaa attcataaga gtttcatttc tgattcacac atttaatatt attttaatgt
    25681 aaaataataa aggtgaagta ttgcacaatt aaaattaagg agggtattaa tgtctgaaga
    25741 aaaaaatcca aaagaaaata cattagatga atctaaaaat tcagataatg taaattctaa
    25801 aaaagatgat gtgtcagatg aaaaaaatac tgataacaaa gttaaaaaaa gggaaataaa
    25861 gctaaacaac aaaacactta aagctattgt agcaattgtt gcagtaggag ctatatgttt
    25921 aggtggaggc tatgtttttg gcaaagaagc tggaagaaaa ttgcctgcta cttcaaaaca
    25981 ctataatagc agtgaggtag tagctactat tggggataaa aaaataacag gtgaaatgtt
    26041 aagaaagaga atggagccat tattttattt aaatgcaaaa actactatga ctgatgacga
    26101 aatacgttca tatgaatcaa atatgattga aactatgatt acatctgata tattatcctc
    26161 tgaagcagca aaagagaaaa taagtataac taatgaccaa ctagatgctc agtataataa
    26221 tttaatgagt agtatagaat cagcaatggg aatgactaaa gaagagtttt taaaacagtt
    26281 tgatttgact gaaaaatata taaaagatga tatgaaaaaa gagctagtag catcaaaata
    26341 tttaaatgaa aaatcaaaag ttactgataa agaagcagag aactattaca ataaaaacaa
    26401 gagcaattat ttacaagtta gagcttcaca tattttaata aaaactgttg atgataaagg
    26461 taagcaagtt agtagtagca aaaaagctga acttaagaaa gaagctgaag aaatacttaa
    26521 aaaagcccaa gctggggaag attttgctac tctagctaaa aaatactctg aagatagtag
    26581 tgcagaatca ggtggagatt taggtttctt tggtaaaggt cagatggtgg aatcttttga
    26641 aaaagcagcc tttgcattga agaagggaga ggtatctaac aaacttgtag aaagtgatta
    26701 tggatatcat ataattaaga aaactgatga aaaatatcag ccatttgaag aggtaaaatc
    26761 agatttagta agcagtttaa catcagaaaa acaaaaccta cttatacaaa atctaaaaga
    26821 aaaatataat gttaaaatta cagataaata tcaaatgaaa gctaaagaat agataaagtc
    26881 aatttattaa aaaaaagaaa aattaactgt atggataaaa gctattttta ctctcaatat
    26941 agagagtata aaatagcttt tattattttg caataaaatc tatttatata tatgaagtga
    27001 taaagaaata acatgcaaaa aggtattaaa aagaaatatt tttaatttga attttttcaa
    27061 taaaaattta caaaaatgaa aaaataaaca aaaaagaatt gaagatttaa gttgttattt
    27121 aaatatatat atattaagat tttctgctga agatactaaa aaaataatat ttttcataat
    27181 tttaaaaata ataataaaaa aatgaaaata caggctataa tctattgagt aaacatagaa
    27241 aagatgatat tataagtatg tgctaaaatg gttaaagcaa tattaatatt aaatttaact
    27301 ataaaacaaa agggggcgtt tttttatgtc aagtacagaa agcgtatcta aaaaaagtgg
    27361 tcatccgaag agtttttggc ttatttgcat gacaattttg tgggaaagat ttagttatta
    27421 tggtttaaca gctttattgg tattattttt tacagcaagt gtagctcaag gtggtttagg
    27481 tctttctact caagaagcaa catctatgtt tggcttattt acaggtctta tatatcttac
    27541 accattagtc ggtggctggt tagcagataa agttttaggt caacaaaaat gtatttctat
    27601 aggtgcactt ttaatagcaa taggagattt cgtactattt gcttcatcta catctattgc
    27661 aatggtttgg gtaggtctat caataattat tttaggaaat ggttttttta aatcaagttg
    27721 ctcaaatata gttggggata tatatcctaa aactgatgtg tcaagaaaag atgcagcata
    27781 cagtttattc tatatggcag taaatgtagg agcatttata gctccaataa tatgtggatt
    27841 gctttctgat aatgtatttg cagtaagagg agcaagtgga gatatagtat cttatggata
    27901 taagatggca tttttaattt gtggtatagg catgatgtta ggtatgatta tatttacact
    27961 tcttgcacca aaattattaa atgaagtcgg aaaatatcct gtgactaaag gtaataaagg
    28021 tgaaaaaatt gagtatggac cacttactaa gttggagaaa aataggataa tagcaatggt
    28081 tattttattt ttatttgtgg tcttattctg gacagcatac tatcaaacac aaagttcttt
    28141 tatgatttat gtaagggata atgtaaatag aactctattt ggatttgaaa tgccagtagc
    28201 atggcttaca tcactaaatg ctatattatg tgtggcttta tgcccagtat tagctaccct
    28261 atgggttaaa ttatctcata ccaaaagagg tgatttctct attccagtta aaatgggatt
    28321 aggaatgata ataatgggtg ttgggtttat agttatggta tttgctacat tagctaatgg
    28381 tccagatgga atggtaaagg ctagtatatt gtttatagta ttgacttata tatttacaac
    28441 agtaggtgaa ttattcttat ctcctatagg acttgcaatg ttcaataaat tagctccagc
    28501 taagtatgct acgttgtcta tgggagcctg gtatctaaca agtttttttg caagtttatt
    28561 atcaggaaag attgctggtt ttacagccac acttggtttt ttacagatat ttggaggaat
    28621 agcaacagtt ttaataatat ttggtttaat attattggga ataagaaatg tattaacaag
    28681 attaatggct atggatttac taacaaaaga aaaataattc atgacgaatt attgtaaata
    28741 aatgaattac tttaaataaa ggaagttaca tttaaatata aaattaaatg taatttcctc
    28801 tttgattatg aaaaaaagct ttacacaaat agatattata attaaaatta taattgtgat
    28861 aattagagtg tataagattt atataaatta tacaaaaaat atttatgtaa taaactttac
    28921 atacttatat atttagttat tcaaactatt atttcgagga ggtcttgttg tgaatataaa
    28981 agatagatta agtggattaa gaaaatttat ggaagaaaaa aatatagatg cctatatgat
    29041 tccatcttct gataatcacc aaagtgaata tgtaggagat tacttcaaat caagagaatt
    29101 tatatcaggg tttaatggtt cagcagggac ggttattgtg actaaagacg aagctgggtt
    29161 atggacagat ggaagatatt ttatacaggc agaatctcaa ttagaaggaa gtacaataaa
    29221 attattcaaa atgggacaag aaggttgccc aactacagat gaatatttat ataaaaatat
    29281 tcctgaagga ggaactttag gatttgatgg tagagttata tctgctagag aaggagctac
    29341 attagctgaa aaattatcta aaaaaggtat aaagatagaa tatcagtatg atttaataga
    29401 tagtatatgg ccagatagac ctgctttatc agatagtaaa gcatttttat tagatgttaa
    29461 atattgtgga gaaagtttta gctcaaaatt agctagatta agagaaaaaa tgagtgaaaa
    29521 aggtacttct actcatgtaa taactactct tgatgatata gcatggttat tcaatataag
    29581 aggtggagat gttaaatata atccagttgt actttcttat gcagtaataa ctttaaaaga
    29641 agtttatcta tttgttgatg aaagtaagtt aaatgaagaa atactaaatg aactagctaa
    29701 ggaaaatgtt caaataaagc catataatga tgtgtatgaa tttgtaaaaa atatagacaa
    29761 aactgaaaaa gttttattag atggaactaa gcttagttat acaatttaca ataatatacc
    29821 ttgtgaagtt gagaaagttg atgagttcaa tccagtaatg ttttttaaag ctcaaaaaaa
    29881 tgaggtagaa ttagaaaata taagaaattc acatgtaaaa gatggtgtag ctttcactaa
    29941 atttatgtac tggttaaaga aaaatgtagg taagatggaa atcactgaaa taagtgctac
    30001 tcaaaaatta gaggacttaa gaagagagca agaaggtttc tttgagccaa gtttcaatac
    30061 aattgctgca tataaagagc atgctgcaat gatgcactat agtgcaactc cagagtcaaa
    30121 ttataaatta gaggcagaag gattattttt agtagattca ggtggacaat attatgatgg
    30181 aactactgat ataactagga ctacagtttt aggacctata agtgatgaat taaaactaca
    30241 ctttacttct gtagcaagag gaatgataaa tctttctaag gctaagttct tgcatggttg
    30301 tagaggatat aacttagaca tactatctag aagttgcatg tggaatatgg gaattgatta
    30361 tcaatgtgga actggacatg gtataggatt tgtgctaaat gttcacgaag ctccaaatgg
    30421 atttagatgg agagtagtac ctgaaagatt tgatagtgca gttcttgaag aaggaatggt
    30481 aactactaat gaaccaggta tatatataga aggttctcat ggtataagaa cagagaatga
    30541 aattgtagtt agaaaagctg aaaagaattt ctatggtcaa tttatggaat ttgaagttgt
    30601 tacgttagct cctattgact tagatggtat agcaccagaa ttaatgaata aggatgaaaa
    30661 agattattta aattggtatc ataagttagt ttatgataaa atatcacctt tcttaactga
    30721 tgaagaaaga gaatggttaa aagtttacac tagagctata tagtttgtac tagatacaat
    30781 aaaaatcgag gtattataat gcctcgattt tttattgtat attttattaa ctttaaaaaa
    30841 tttttaattt gataattcta ctaatttttt attcattata agtagtataa ttccaaatat
    30901 caataaaaat gatggtatca ctatgaagac ttgtaacata ccaagttggt ctataatagc
    30961 ttgcgtatag ccagataatt tatttgctgc aaatttgcct aaaaaccaaa cacccattag
    31021 gaatgaaata tacttttcag gcgaaagttt atttaccata ccatacccga ctggagaaaa
    31081 gcaaatttct cctatacttt ggaatgttaa aaatccaact acccaaaata cacttgcttt
    31141 gtgaattgaa ttagggtctg ttcctgtttg tataactgct actatcataa acaaaaatgc
    31201 tatggctaat agtataaaac ctaagctcat tttttttggc acacttaaat cgcctttttt
    31261 agtttgagat aattttaccc atactaaagc agagataggt ccaagaataa cacaaaggag
    31321 accattgtat gaatctatcc atgaagtagg aatttgaaaa ttgccaacag taagatttat
    31381 gtgttccttt gtatagagtg caatagacat agaggcttgg ttatatgcta tccaaaaaaa
    31441 tactgtgaaa aaagctaaca ccaatattac tattattctt tttttctcaa tcaaagttaa
    31501 aggtcttgaa gcgagcattt tcttatattc tagtgatttt cttatagact ctctcccgtt
    31561 aatagggtca ttattcaaag gtttataaaa ctttctgttt ccaaatataa agaagaataa
    31621 acctattaga accgtaattg atgaaaataa aaatacatat ctatatccat acatacttat
    31681 agataatccg cttttcatag caaaccattc attagcaact aaaccgcata ttaaagggcc
    31741 aaataaagta cctagattag tagccatata cgtaatagag aaagctccat cttttcttgg
    31801 gtcatcattg gtatataagt ttccaaccat agtatgtaag tttcctttat aaaaacctat
    31861 tcctaatcca gataaaaata tcatgatgtt tatatccatc ttactatttg ctatgtatcc
    31921 tagaaaataa ccaattgaaa ccattagcat tccaagtata atacagtttt tgggacctaa
    31981 aaatctatca ctgacccatc caccaataac tggagagaaa taagtccatg ctaaaaaaga
    32041 tgcaagcatt gatgcggatt ctgctggtgt taaccctaaa ccacctttgg agacttgttc
    32101 agctaaaaac aataataata ccaatttcat accatagtat gcaagtccta caaaggtaaa
    32161 tattgaacag cagaaatata aatctctagg ttgtttaagt ttttcttgcg tttttaaaac
    32221 tgacatatgt cgagaacctc cagataaata gtttaacaaa attttaccat actatatgag
    32281 tatatgtttt tttcatatag acgtcaactt gtaagataat ttaattaagt cgaaatatat
    32341 gtaaaaatag tatcattttt gtgtattcag taatattttt ttatgataat atatgttatt
    32401 attatattaa aatacaaaca tatttatgga aaggatatgc caatttagta gttagattga
    32461 ttgtttggca ggtgggatga gctatgataa gaaagaaatc tgaattacaa gtagaagtaa
    32521 aagaaaattt aaatggtggt ataggaaagg ttaagcttga aaacataata caaaatgaag
    32581 agcttaaagg aaaaggaaga ctatttaaaa gagtagcttt accagtagga agttcaattg
    32641 gtgtgcatga ccatacaaca gattttgaag tatattatat actaaaggga aaaggtaaag
    32701 tatttgataa tggggaattt gtagaagtaa atgagggtga tgtagtgtat acagctgatg
    32761 gcgaaaaaca ttctatagaa aatataggag aagaagatct tgagtttgta gcactagttt
    32821 tatatatgta aaatatggag gaggagtcat gacaggcaag atagaagaaa ttaaggacaa
    32881 gataaataga ttttgcaact ccaatttaaa tcaagaatac aaagatataa gctttaaaat
    32941 tttgcaaaac cttttagata ataataaaga gataattcat tcttctagag ctgatatatg
    33001 gtcttcagct atactaaata tagttttaga acaaaatctt ttatacaata agaagcatcc
    33061 actacatata acaaaaaaag agttttcaaa acaaataggt gtttctttaa atactataaa
    33121 taataaatcg tctttaataa aagatgtttg tagtattgac tttttaaatc aagatactat
    33181 agctatttct aatgtagaat tttgggtaaa agagactaat tccaaatcaa agtctgtaga
    33241 tttagattta gaaaagaaga aaatactata taaaagatat attaagctat cacaagatgc
    33301 gaatagtgat attgaatcaa ttagatatat ggaggaagct gtaaatatag gtaaaagtat
    33361 gattactaaa gaagataaag aacttggatt ttggaaaggt ttatctacca gagcctatat
    33421 gatagcactt gaatcccttg ctagaaagtt agaaagtatt aataatctaa aggagtccaa
    33481 aaaagtatta gaatatttaa tagaaattaa tcctagtgat gagcagggaa taagatacaa
    33541 attattaaat gtattaatta gattaaatga tagaacttca ataaataaat tatttgaact
    33601 ttataaagaa gaaaaatctg ctacatggat gtacagtaaa gctctttatt actttaaaaa
    33661 taaaagtatg tttttggcaa atgattctat taaggttgct agaagtaaaa ataagtacgt
    33721 tggattatat ttgttagatt ggcgaaatgc ttttggtaga gaatttgtta cagaggaaga
    33781 aaaggcagaa gctgtctatt actatgatga aaacatagtg atttggaatg aagttaaagg
    33841 ttctatggat tggctattaa aaaaaatgtt ggaattttca taaaaaaaac tactccttac
    33901 ttttatataa gtcaaaggag tagtttttaa tttatacatc tagttttaaa tcttcaggtt
    33961 ttattagacc tttttttctc ataaattctg aaatcaacaa agttacaata gcaggaagta
    34021 taaaattaag taaaagtata ccaacccaca tagatatacc tccttttcca gcactttgca
    34081 tagcagttac tgttccaaat tgtccaacca atccacaagt acccatacca gaaccaatag
    34141 gaatattctc cattttgaat attgttgtag ataatggtcc aagtaatatt gaagctacag
    34201 taggtggtat ccaaattttc caatttttta ttatattagg tacttgcaac atagatgtcc
    34261 caagaccttg tgccacaagg ccaccaactc cattttctct aaagctcata acagcaaagc
    34321 ctaccatttg gcaacaacaa cctacagttg ctgcaccacc agcaagacca cctaaactta
    34381 acatcataca aatagctgca ctacttatag gtaatgtaag agctattcca acaagtgcag
    34441 agactaaagc tcccattatt ataggttgca tagttgtagc ttgcattatt atatttccaa
    34501 atgaagtcat aaaagcagaa acagctggac caataagttc agctacaagt actccaatta
    34561 gtattgttac agcaggtgta accaatatat ctatttttgt ttcttttgaa actaattttc
    34621 caacctctac agcaaaaagt gtagcgacaa atgctccaac aggtcctcct aattcattac
    34681 cacaagctcc aacaagtgca caagaaaata gtacaagtgg aggtgcttga agtccatacg
    34741 caatagccac tgctatagct ggaccagtaa cctgtttagc aagagggctt ataacttcag
    34801 taaaaagaga aatattgaaa ttatctccta atgtattgaa aattgttcca attaaaaggg
    34861 acgaaaaaag accgagtgcc atataactta gtgcgtcaat aagatacctt tgaacagaaa
    34921 tttcaatatt ttttctttta gcaaatgatt tgaaatttct ttttttataa ttgatttcca
    34981 atttaaaatc ctcctcatat aatataagta taatttcttt ttaattaatt atataacttt
    35041 tcagctgaca agacaaatgt tatttcacta aaattagact attgtaatag taagaaatta
    35101 acattaattt tagttttaaa ttttaaattt atggtttata attagtatag ggatattctg
    35161 aaatataata attttccagt ttatcccaat atttaactta ggtagattgc ttacaaaaca
    35221 aatgattata tattaggcta aaaggaagtg gatagatgat atttgtaaaa aataagaaat
    35281 acagaaaaat tgccataatt atatctattc ctatattgct tgttgcgcag tactaccttt
    35341 ttaaatttgg aatacttaga ccaatggtaa agggagtaga aattgaaatt gtagatgggg
    35401 aatatgttaa agatatagat aaatatgtag taaaattaaa tgaaacagtt actttgtcta
    35461 caggtgaata tattaaggtt ccttcgtatg ctaaaaatcc aaatatttgg tttaatgtat
    35521 tggataactc agggacttta aaaatagatg gaaataaaat gacagcatta aaggttgggt
    35581 attcttctgt gggtatcatg aaaaattcaa gagtattaaa aaaggctact ataaaagtag
    35641 tagacccaga ggtagaatca ctagatatgg agattaatgg ggatttaaaa catgttggtg
    35701 atagtgctac tatagaaagt acaatagaag ttgattatga taagtttaaa aaatcatata
    35761 agccagttta taagagtagc aatgaaaata ttttaaaaat caaaggaaat aaagtaagtg
    35821 cagttggagt tggtaaagct acaatatatg ctaagtgtgg aaataaagaa attgagagaa
    35881 catttaggat tcaagctaga gttgctgaaa taaatatgga tgatataaaa atagaagttg
    35941 accagaatga aaaattgaaa ccagatataa taacttctcc gcctggttta gaaccaccaa
    36001 aaatatatta tgagttagta gattcaaaat tacctgtaga aagagcaata agtttaagta
    36061 gtaatggaaa tgttgtaggg cttagagaag gtaaagatag ggtgagaata acttgtggag
    36121 ataaatctaa agtaataaca gtaacagtag tcaaagaaac tataacgaat gactatatac
    36181 aaaacttggt tggttcacat aaaatagtaa acaataaagt tattattaaa cttgaatggg
    36241 attatatgaa agatgtcttt gattatgata tatatcttaa agataagttg tcaggagaat
    36301 catttaagaa aattaaatct ataactataa atgaaagtga tttaggtaag tctaacaagg
    36361 tatatgcaac tatagaagta gatatgaagg gtaatcaaaa tatagatttt gatatttatg
    36421 tcatagggaa aaaggattca gatacaacta agccaagtaa tgtaataaat attaccaatg
    36481 gaaatggaaa ttcaaataat gataataata ttaaagatat gagggtagaa aatatatcag
    36541 ctaatgttga tagagataat aatgtcatta gagttaactg gagtagtata tctcataagg
    36601 actgtactta cagtatatat gtaaaaaata atactaatgg agatggagaa tttactctat
    36661 atcaaagtga tatacaggga aatgagcata ctataagtat tcctgaagga gatttaaatc
    36721 tagatattta tgttgttgca aattataatg gaaatagctc tcaaaatagt gatgttataa
    36781 atgtaagata gatttgtata caaattgaaa gtgttgagtt tatttaaata ttaaattaag
    36841 aaaaaggata gtatagggta attatactat ccttttttgt tacaatatat aggtttaaaa
    36901 tctctagtaa tataaattat aaaaagtctt atttacacaa atctatagat atttttttta
    36961 ttaatcaata ttgtcttaaa ttgaacttag tgcggcttct attgcatgta ttactgtcgt
    37021 atcaaatagt ggaatagaag tgtcattttg tttaattaaa agacctattt ctgtacatcc
    37081 taatataaca gcctctgcac cttgtttgcc aagcttatct ataatagata agaaagcttt
    37141 ttttgaagat tcagatataa ttcctaagca aagttcatta aatattgtgt catttactat
    37201 tatacggtct tcttcatttg gaattaagac ttcaattcca ttattaataa ttacattttt
    37261 ataaaaatct tgttccattg tatatttagt acctaataag gccacttttt ttatttcttt
    37321 ttcccttaat accgtagcag ttacatctgc tatgtgaagt aatggaatat gtatactttc
    37381 ttgtatttta tctgatacct tatgcatagt attagtacag ataacaataa aatctgcccc
    37441 tgcctcttgt aatttaattg cagcatcagc taaaattttg gcgctttttt cccaatttcc
    37501 actagattgg cattcttcaa tttcttgaaa atctacactg taaagtatac atttagaaga
    37561 gtgtagtcca cctaatcttt cttttattac tgtattaata acttgataat atgtaatggt
    37621 actttcccaa ctcataccac caattaagcc tatagtcttc atattatacc tctcttattt
    37681 atttattttg aaatagctat ttacaaatac aagtatagca tctaaaatag aagcatacaa
    37741 tgataaaatt gtatggagta caattataat taagaggagt aattatatgc caataaattc
    37801 ttttgaaaac tatcctatga catggaaacc aaaattagat aaccgaaaac caccaatata
    37861 taaaacttta gcaatgttat tagaagaaga tattaaaaga ggaaatttaa atcctggaga
    37921 taaactgcct ccacaacggg aattagcaga ttttctagat ttaaacttaa gtacaataac
    37981 acgagcattt aaactttgcg aagaaaaggg actaatatgt gcaaaagtgg gaaagggaac
    38041 attcatttct tctgatgtaa atgtaagtaa tacattatta tatcaaacgg aatcaaaaga
    38101 cataattgaa ttagggacag ttcatcctcc gtatgagcaa aatacatata ttattgactt
    38161 tattaaaaat gtactaaaac aaccagaaat ggataggttt ttacaataca tgtctccatc
    38221 tggcacatat atgcaaaaaa agtcaattgc aaaatggata gaaagaaata atgtatacac
    38281 cagtgaagaa aatatattat tatctacagg agggcaaaat gctatatgtg caactttact
    38341 aggattattt aaagctggtg atagaattgc tacagattct ttaagttttt ctggaattaa
    38401 gtcaatagct aaaatgatag gtattcaatt agtcccaatt cctcaagaaa ataatgaaat
    38461 atcgattgaa tatcttgaaa gttattgtaa aaatgaaaat ataaaaggca tatatcttat
    38521 accagattat cataatccaa cgacacatac tatgtctgat ttatctcgta aaaagatagc
    38581 caagatagca aagcaatata atataataat tattgaagat gctataaata gcatttttag
    38641 agatggtata caaactccca tattttcttt agctagtgat aatactatat atatttttag
    38701 tacatctaaa tttttatgtg ctggtcttag agtagcattt gtagttgctc caaaacgata
    38761 tatagaaaat ttagaaaatg cactatataa tatgaatcta atggtttctc catttacagc
    38821 agaaatagta caccgtttat tatattctcc tattatagat aaaattatag aagagaaaag
    38881 agatgcaatt atagaaagaa atgaagttgc tgataaagtt ttgtcagatt ataatttgat
    38941 tggtgataag aattgcagtt ttcgttggtt acttcttcct gatgaattgg atggaaaatc
    39001 atttgaaatt tctgcaaaaa atctaggagt tcaagtatat tgtgctgaac gattttcagt
    39061 aggaaactct acagttccaa aagcagttcg tatttgtgta acagctccaa aagatgtaga
    39121 agaattagaa aaagggctta atataataaa gtcacttctt aagagttaaa tttattttac
    39181 tattatttaa ttaaataaat aaaaaggcgg agaaatttat tataataaac ctccgctttt
    39241 attatttaat gaaatacttt actttttatt aaaaaattta attttaataa tcttgaaaat
    39301 ataatcgttt tatggtaaaa taattcttaa aaatagaaaa aattaatcat ttgttaaaaa
    39361 aaatgcataa tttattgtaa tttattgacg aaggttgaaa aatattcata aatgtgcgaa
    39421 ataacagaaa aaatgcaaaa attaattcaa tttcatttgt tataaaatct atatggcaaa
    39481 attaaatctg cctatcagaa tgatataaac acaccagaaa ggaggacgct atcaaataat
    39541 acaagtagtt aactttgata gcaaaaaatt atgaatgaac aaactagaat atccttagag
    39601 agagctgctg aattaaaatc aaaaattgat gattatattc aggctagaaa aacgattaac
    39661 agaggtcttg aaaaagaaga agagataaat aaacgaaaac agaaaatatt aagtatctta
    39721 aatggaactg aagaggattg gaataactac aaatggcaat tatcaaatag aataacagat
    39781 gtagatactt tatcaaaaat tataactcta actaaaaaag aaaaagaata tataaaagag
    39841 gttggtactc aatttagatg ggcaatatct ccatattatt tgagtcttat agacccagaa
    39901 gatacatgtg acccaataaa attactgtct ataccaacac atatagagtt ggaagatgaa
    39961 caagaagatt tggacccaat gggagaagag tatacaaacc cagcaggatg tataactaga
    40021 agatacccgg atagattaat aataaatgta acaaatgagt gtgctatgta ttgtagacac
    40081 tgtcagagaa gaagaaatat tggacaacaa gattctcata agtcaaaagc tattatccaa
    40141 gaatctatag actatatcag agaaaatgaa gaaataagag atgtactagt aactggtgga
    40201 gatgctctta cattaaaaga tgattattta gagtggattc ttagccaact taaagagata
    40261 ccacatgttg attatgttag attaggtact aggactcttg ttacaatgcc acaaagaatc
    40321 acagatgaat tttgcaatat gctaaaaaaa tatcaccctg tatatataaa tactcatttt
    40381 aatcatccaa tggaaataac taaggaatct aaagaagctt gtgaaaagtt agcaaatgca
    40441 ggagttccat taggaaatca ggcagtatta ttaaatggaa taaataatga taaatttgta
    40501 atgagatgtt taaatcaaga attactgaaa ataagagtaa aaccttatta tatattccaa
    40561 agtaaacatg taaagggaac aaaacatttc aatacatcag tagatgatgg tcttgaaatc
    40621 atggagtatt taagaggata tacatcagga atggctatac caacatatat agtaaatgct
    40681 ccaaaaggag gcggaaagac tcctttgctt cctcaatacc ttgtatcaaa aggaacagat
    40741 tacgttatgc ttagaacatg ggaaggaaaa gttataaaaa tggaagatga acctgctgta
    40801 gatataaaga aacttataaa agaacaagcg caggattaaa aaagtattta aagtttataa
    40861 aaagaaacaa ctttaaaata aaaagaaaat ggatgtcaaa tttatatcaa tttgcatcca
    40921 ttttttatta gttatttatt attttgttgt tgattaacta attcaagata tccacttggg
    40981 tggaagtaat atatctcaga agtattttca tttacaaagt aaccaatatc accatctgca
    41041 tctggtaaaa atacataacc attaagattt ttagctttta atgcttcaaa atcactagca
    41101 tttccttgat atatgtaatc aacttcattg ttgtatttta ctaaaagttc tttagcttgt
    41161 tcttgagtca atttagtatt ttggcttaca ggaacattct tttgagaatc tgcataagaa
    41221 gattcattta ctggtgcagt taataacaaa caagataaaa ctaataaaga tataagtgct
    41281 tttttcatac taaaaccctc tttcatgttt aattatataa gattcattac ttaataatat
    41341 tgtatgtttc ctataattaa atattaccat taaagagaat aatattacag ttacacagct
    41401 gtaacttttg attacaaaat cgtaattaaa ttgtattttt attccatttg tattttatca
    41461 tgtctacgat ttacaaactc acagaagtat atctttataa cgcccatcac aggagcagat
    41521 agaatcatac caggtatacc aaagaatcct ccaccaacag taactgctag gattgtaaag
    41581 aatgggctaa gacctagttg tccacctacg atttttggct ctataaatgt aacttcaact
    41641 tgttgaatta ttattaagta aattaaagaa gttaaagcaa ttgttggatt ggagaataaa
    41701 ttaattatta caactggtgc catacctatt actggaccaa aatatggaac catattcatg
    41761 acacccataa gtgtaccaaa taatagtgca tattcagatt ttaaaaagta aagaccaatt
    41821 ccagaaagta ctccaactat aaaagaatct aaaatcttac ctgtaaagta tttaccaata
    41881 tttaaattta agctttgaca tagttctatt ataaagtctc catatttttt acctaaggaa
    41941 atataaacta ctttttttgt aaacagtaag aatttttctt tttctaataa tatatagaaa
    42001 caaattataa aacctaaaag aaattgtact ataaattttc caactgaaaa tgtagtgcta
    42061 aatatcccac caagtgaacc aataagcaag ttactaaatt taggcattac actcataatt
    42121 ttgtcaccat actcttttaa tgtattaggg tcaactgatt gtaaactctt gcctaagtcg
    42181 aataaaaact gctctgtttt atctatgtat gtaggaattt gagcaactaa atcagctaaa
    42241 ctatttacta tggatggaac tgtaaataat ataaacgaaa ttagtataac tatgagtaca
    42301 ccataagtaa gaagtaacga atatattctc ttaaaattta atttactttc taagaacttt
    42361 actagtgggt tgaatatata tgctaataca aatgctatta taaatggtgt tagtagtgac
    42421 attaataaac ttatcactcc aaaaaaatat ttgtaattat caataacttt aatcaagata
    42481 tatgaaacaa ctacagtgag tacaatattt agataaattt ttttatcttg caaaataaca
    42541 caacctttct tatatttatt aaatttagat atacttaaat ctaagttaaa gttaatattt
    42601 atagcttatt atacaataaa aagcagtatt aatatagtat gattaatatg aaattataaa
    42661 tacgttaaaa ctttatgttt atagctaaaa taataaaaat tatttatatt gtgtttatat
    42721 ataatctaaa atgaaaaaaa gtggtgcctt tgattaaact aagtttttat aatttaaatt
    42781 ttgtacaata agctatcttt tattattctt taaaatatat atttcattta attatgacaa
    42841 ataaagataa aaattaaata ttttagaggt gattttattt agatatagaa ttttagaata
    42901 atactataca aatgtattgt attattattt ttatatcaaa aatagtaata aagtctatat
    42961 acatttagaa aatactgttt aaaaggagta ggatttatga taaaaaaaga gatatctaac
    43021 tttagaggaa gtagtgatta tgtagtatca ccagaattga tggcatcagt taatgttgct
    43081 attgctcttg aaaagccact tttgattaag ggcgaacctg gaacaggtaa aactatgcta
    43141 gcacaagcaa tttcaaatga attaaaaaaa gatttagtaa tttggaatat aaaatcaact
    43201 acaaaagcac aagaagggct ttatgtatat gatactgttc aaagacttta tgatagccaa
    43261 tttggaggag aaggtgtaga tgatattagc aagtacataa aatatggtaa actaggagag
    43321 gcctttagct caaatcaaca agtaattctt ttaatagatg aaatagataa ggcggattta
    43381 gaatttccaa atgacttgct ttgggaattg gataaaatgg agttttacat aaatgagaca
    43441 aaagaaactg taagagctaa acaaagacca atagttatta taacttcaaa tgcggaaaaa
    43501 gaacttccag atgctttttt aagaagatgc atatttcatt atatagaatt tcctgataga
    43561 gacatgatgg aagaaatagt taaagtacat tttgataaag tagaagaaca cctattggaa
    43621 caagtaatga ctacttttta ttggattaga agtttaaagg acatacaaaa aaagcctagt
    43681 acatcagagt taatagactg gatacaagca ttaactttga gtggaatgcc tattgaaaaa
    43741 atagaaaaag aagttccttt tgcaggaata ctacttaaga ataatgagga tattgaatct
    43801 atgcaaagac acttatagtt aataggagct ggataattat gtttattgaa tttttttatt
    43861 tattgaggtc aagaggatta aatgtatctt taaatgaatg gatgacttta attgaagcat
    43921 tggataaagg actgtgctat tcaaactttt caaattttta ctatctttgc aggatgattt
    43981 taataaaaag tgaaagtgat tttgataagt ttgatgcagt gtttttggag tattttaaag
    44041 gtatagaaca ccaagaagaa attccagaag agattatgaa ttggcttaaa aaaccagata
    44101 tagatttaga agagtttgaa agattagaac aaaatccaaa tataaatctt gataaattaa
    44161 gagctaaatt ggaggaacga ataaaagaac aagattctcc tcataatggt ggtaattatt
    44221 ggataggcac aggaggcact tcagagttag gacattcagg aaaagggcaa actggtatac
    44281 gtataggtgg aaattctact tatggtagag ctgttcttga agttgcagga gaaagaaaat
    44341 acagagattt tagagatgat gaagttttaa atatgcgtca atttcaagtt gcactaaaga
    44401 gacttcgtca attttctact agaatagatg ctccaaagac agagcttgat ttggataaga
    44461 ctattgaaga gacttgtaat aatgcaggat atttaaagtt attttttgag aagccaagaa
    44521 aaaatacagt gaagctttta ttgcttatgg attcaggtgg ttcaatgaga gggtatagca
    44581 ctttatgtaa tacattattt cagtctgtaa gtaaatcaaa tcattttaaa gatgtaaaga
    44641 tatattattt ccataactgt atttatgata gattatttac aacacctgaa tgttggttaa
    44701 gtaaatctat taatactgaa tggatattaa aaaatataga taaaaactat aaagttataa
    44761 ttgttggaga tgcttctatg tcgccttctg agttgcttca tattggtggt aactatcgtg
    44821 gtccatataa ttatacacct ggtatagagt ggcttaaaag gtttaagaga aagtacagta
    44881 aaattgtatg gatgaatcca gaacttagag atggatggga ttcaattagt tattggtatc
    44941 aaactcaaag aatgatacaa agtgaattta atatgtttcc attgactgta aaaggtcttg
    45001 agaaagcatt gaaaaattta atggtttcaa gataaagtaa atataaatga tggtatgatt
    45061 tattcaaaat tgtaccatca ttatttattt ttttaatata tctattataa attgtataac
    45121 tgaaacttaa aaaattatat agtctaatac attttagact cacacaaaac atatacatta
    45181 ataataattt tagaagtcga ggtgattata ataaattatg aattaattgt aaaatacaat
    45241 ggagatatat tgaggttaga agaagaatta ggtgtatcag tagagatact taattcatca
    45301 tatgcaataa taacatcaag taatgaagaa gatgtaaata tacttttaac ttatccagaa
    45361 attgaattta tagaaaagcc ttttatatta cagactcagg atgtgcaaag tttttcaagt
    45421 acaggaataa caggatttaa aaatagaact ggtttaactg gaaaaggaac tataattggt
    45481 ataattgatt cgggaataga ttatactcta cctgtgttta gagatagtga tggaaggtct
    45541 aagatattat attattggga ccaatctatt caaggaaatc caccagaagg ttttagagaa
    45601 ggaacattat atactaatga agatataaat aatgcaatag atggaagtat gtatatacct
    45661 atttcaacaa ctagcttaca tggaacacat gttgcaggta tttgtgcaac tattgcaagt
    45721 gatgccagaa taatagtggt tagagttgga aatatacaga ccgatatttt ttcgagaagt
    45781 acagagttta tgagggcaat taaatttatt ttagataggg cattagaatt aagaatgcct
    45841 gttacattaa atataagtta tggaagtaat gaaggttcac atagaggaac atctttattt
    45901 gaacaatata tagatgatat gtgtttattt tggaaaaaca atatagttgt agcagctgga
    45961 aataatgcag ataaaggagg tcataaaagg attaggcttc aaaataatat tacagaagaa
    46021 gttgagttta tagttgggga aggagagcgt atattaaaca taaatatatg gccagatttt
    46081 gttgatgatt ttagtgtaca tttggtaaac ccatcaaata atcaaacaca agcaatctct
    46141 ctaacttcag gtgagataag gaatactttg ggtgaaacta gaataactgg ttatttttac
    46201 cctatagcac cgtattctct cacaagaaga gttactctac aattaagttc aaatacacag
    46261 ataactcctg ggttatggaa aattgtattt gaaccaatag atatagtaac tggtaatgta
    46321 aatatatatt tgccaacttc tgaaggtcta aatagaaata ctagatttct gattcctact
    46381 caagagctta ctgtaacagt tcctggaaca gcaagtagag ttataacagt aggtagtttt
    46441 aattcaagaa cagatatagt atctatattt tctggtgaag gagatacaca attaggtgtt
    46501 tttaaacctg atttattagc tccaggtgaa gatatagtat cttttttacc aggaggtaca
    46561 agtggagcat taactggaac tagcatggct acacctcatg ttacaggggt ttgttcattg
    46621 tttatggaat ggggaattgt aaatggaaat gatttatttt tgtattcaca gaaattaagg
    46681 gcattacttc taaagggagc aaggagatta agtaatcaat cttatccaaa taattcatca
    46741 ggatttggtt ttttaaattt atcagatata gatttgtaca ctctatctaa tataaatcaa
    46801 gatttagaaa cagaggatat tggatataga agtattaata agtcatttaa agatgaggaa
    46861 aatagttata agtttataga tggttataat atgcaaatac ataatgactt agagaatgaa
    46921 atttatataa gtaaaaatgc aagtagacaa tcaggaatac tatcaggaat agatatagtc
    46981 catactcctg aatttgaaga agaactagct gggttaggta tgagtcaaag gttttttaag
    47041 ataagtgatt cattaggtgt actatctata aataatacag actataatag catacaacga
    47101 gttttgcaac ttccttcaat cataagaact gtatcgacta caaagatgac attacttgga
    47161 gaaattaatc gaggtacttt tggaggtgta gttgcaacag aagaaatggg cgtaaatttc
    47221 tttaaaaata atcctaatat taatataaca ggaagaggga ctctaatatc aattgcagat
    47281 acaggaatag actatctaca cccagatttt atttatccag atggcacttc aaaaatagta
    47341 tatttatggg accaaacaaa agaaggaact cctcctgatg gtttttatat tgggacagaa
    47401 tatacacgag aagatataaa tcgagctatc gcagaaaatg accctagtct atctcaggat
    47461 gaagttggtc aaggaactat gttgagtggg atatgtgcag ggcttggaaa tgtaaatagt
    47521 gaatatgcag gtatagcaga agattctgag ctaataataa taaaactcgg caagatagat
    47581 gatttttata atagtgcaat gttgtttgct gcatctcagt atgcatataa aaaagctttt
    47641 gaacttggaa gaccattagt tataaatatg tctttaggta ccagtagcct agcaggttta
    47701 accaatagga gtaatagtga aaaagcgttc tttactagag ggttatgtat aacggcagga
    47761 gcaggaaatg agggcaatac acagacacat acatctggaa taataccata tgttggaggt
    47821 tctgttgaag ttgaattaga gttaaatgaa gacgaggaag aattatcatt agaactttgg
    47881 ttaaatagac cagataaagc tgatgtaata atagtatctc ccactggtga agaaagtaaa
    47941 agtgttggaa tttcaaatta taataaagta acaggtttat ttgatttaga ggggactgaa
    48001 tattctataa catatatata cccaacaact ttttctggac agcaatttac taatgttact
    48061 ttaaagaatg caaaaagagg tgtatggaag attcgattag taggagttta tattataaca
    48121 ggaagatata acttatacct tccaaataga gaacttttaa aaagtggaac tagatttaga
    48181 gaggtagacc ctttttacac tataaattat cctgcgattc aagatgattt aataaccata
    48241 ggagcctaca ataccattaa tggtagttta tggcaaagtt catcaagagg gccaactatt
    48301 gaagacaggt taaaaccaga tatagtagca ccaggtgtca atataatagc tgcttatcct
    48361 gggaacacgt atgcaactat aactggtaca gcagcagcaa gtgcacatgc agctggtgct
    48421 gctgcaatgt attttcaata tacttttgtt gacggtagat atccaaacca agcatatgtg
    48481 caaaaaataa aaacttttat gcaagctggt gctagaaaag attcaaacac agtatatcca
    48541 aatacaaatt ctggatatgg gctcttggat gttagaggaa tgtttgatgt tttaagatag
    48601 gagggattta tggaaaaatc ttattgtata atttatcaag gtgatataga atctgcccta
    48661 caagaaaatg gaataaatag gtatatggtt ttaaatagtc agcttgcagt aatatatgta
    48721 cctttagatt ttgatgaaac tattttaaat aatataatac aagttgcttg gtgggaagaa
    48781 tctgaaccaa tgagcagtct aattgaaata actaataatg taaataatgg agaaaccatt
    48841 acaacagcag ctgaaactga ttatatttat gaaaatccat ataatgatat aacagggaga
    48901 ggaattttat tagcagttat agattctggg atagattatt tgcatcctga ttttataaat
    48961 gatgatggaa ctagtaaagt tttatattta tgggaccaag aagcaaatac aaatccacct
    49021 ccagaaggtt ttatttttgg aagtgaattt acaagaagtg agttaaatat agccataaat
    49081 agaaatgatg ggagtttgag tcaagataat ataggtactg gaactttagt ttctggtata
    49141 ttagttggaa atggaaggat aaattctcag tacagaggta ttactacaga atcagactta
    49201 atagtagtta aattaaaatc atatacagat acttattatg ctggaaggat aaattacagt
    49261 gtttcagatt ttttagcagc tataacgtat gttactaaca tagcaagaac agaaaataaa
    49321 ccactgataa taaatttgac tattggagtt aagtcaagtg ctgtagcaac tacttcaata
    49381 ctagatactt ttaatatatt gagtagtgct ggtgtggtag tagtgagtgg tgcaggaaat
    49441 caagggaata cagatattca ttattctggc agatttagca gtgtgggaga agttcaagat
    49501 gtaataatac aagatggaga tgactatgct cttgatataa cactaaatac gaatggtcca
    49561 gataaagtgg gagcacagat aatatctcct tcaggtgaag ttagtcatga tataaggtat
    49621 tctccagact tttatatata tagaggaaaa tttaacttag aaaatactac ttatgcaatg
    49681 agatttattt atccatatat tacctctgga aaagaaaatt tggagataag attaagagat
    49741 ataaaacctg gagtatggat tttaagattg acttcagaac ttataataaa tggagaatat
    49801 gacatatact tgccaaataa aaatttgata gctccagaca caaggttttt agagcctgat
    49861 tcagtagcta ctataactat gtatgctgct agtgatgatg taattacagt tggtacattt
    49921 aacaataaga ctgatagtat gtggattggt tcttcgaaag gaccaattag aggaagagga
    49981 attaagccag atatagtagc ttcaggtgta gatataatat ccacatataa aaatggaaca
    50041 tataatacag gaacaggaac aggagtgagc agttctatag tcacaggtgt tttagcctta
    50101 ttgatggaat acttagaaaa acaggataat gtaccgagat tatcattgtt tacacaagtt
    50161 ttaaaaacat atttaatatt gggtgctaca aaacttgaga tatatacata tccaaatgta
    50221 tcgcagggat atgggatttt aaatttaaaa aatacgattc aacagatagc aaatactcta
    50281 tagataagaa cctatgtaaa attataattg attattagtt taattgtatc aaatacttta
    50341 gatagagtag aaaaatagca taaaaaataa atatgaaaat taaaaaacag ttttatattt
    50401 tgaatacaca aagtaaatat tttgtatata aataggtata aattaagtat tgaaaagata
    50461 ttatgttaat ttaaaaataa ttataattta gacttatgaa ttttatttgt aaaattgcta
    50521 aaactattac aaaataaata atagaggtga tagttttgga aagtaaaaag gcaagagcat
    50581 taaaaggtaa agataataaa agacttaaaa gttcactttt agttggaaat gagtcgacag
    50641 cagcatgggc tgatgttgaa aaattaaaac ctcatagtaa agtttcaata ccttcaatta
    50701 agggtgtcga agaagcaaaa gactgggttg acaatggaag taaattataa aattttggaa
    50761 attgaagtaa taaagaagta atgaagataa gtatttatta tcattatgta aggtagaatt
    50821 attatatttt aaataacaat cagtatcaat tatataaatt tattgactaa aaatttataa
    50881 attggtataa ttttgttaat tagcagaaat aataaaagat aaatactgtg aagagaagag
    50941 tagatataaa atgtagttct agcgagttga ggatggtgaa agctcaatac gaggtttata
    51001 ttgaagaaca tcttggagtt tctaatcgaa agcttattta gtaagtagac ttagacggaa
    51061 ggcaccgtta taggctttaa gcattgctta actaagaggt cattatgaca actagggtgg
    51121 taccgcgaga gtaatagtct ttcgtccctt tatataggga tgaagggctt tttttattga
    51181 aattatattt aaaagaaatt aataacaatt atactaggag gatttaaaat gcaaaaggaa
    51241 tttataacag taaaagaatt gtacagagaa aaagaacagt acatagggaa gactgtaaaa
    51301 gtagctggat ggataagaac atctagagta tctaaaaatt ttggatttat agagctaaat
    51361 gatggaagct tctttaaaaa tatgcagata gtatttgatg agaaagtaga aaattttaaa
    51421 gaggtaggaa aattagcaat aagctcatct atacttgtag agggagagtt agtatctaca
    51481 gaaggggcta aacaaccagt cgaaatacat gcaaaaagca tagttgtaga aggggaatca
    51541 gatagttcgt atccactaca aaagaagaga catacgcttg aatatttaag aacgatagca
    51601 catttaagac caagaagtaa tactttttca gctgtattta gagtaagatc aatagctgcc
    51661 tatgcaatac acaaattctt ccaagaaaga aactttgtat atgcacattc tccaatcata
    51721 acaggtagtg actgtgaagg tgcaggagag atgtttagaa taacaactat ggatttaaat
    51781 gatattccaa aaactgaaga tggcaaaata gactatactc aagatttctt tggaaaagag
    51841 gctaatttaa cagttagtgg tcaattaaat gccgagataa tggcactttc ttttagaaac
    51901 gtttatactt ttggacctac atttagagct gaaaactctt atacaggaag acatgcttca
    51961 gaattttgga tgatagagcc agaaatagtt tttgcagacc ttgaagataa catggaactt
    52021 gcagaagata tgataaaata tgttataagc tatgtattag aaaatgctcc tgaagaaatg
    52081 gaatttttta atagttttat agataaagga ttgttagaaa gattaaataa gatagtaaac
    52141 tctgagttta ttagaataac gtatactaaa gcagtggaat tattaattga atctgggcat
    52201 gaatttgagt atcctgttga gtggggatgt gaccttcaaa cagagcatga aagatatata
    52261 acggagcaaa tatataattg tccagtgttt gttacagatt atccaaaaga tataaaagct
    52321 ttctatatga gaatgaatga agatggtaaa actgttagag caatggattt actagttcct
    52381 ggagttggag aaatcgtagg aggttctcaa agagaagaaa gagaagatgt attgttagat
    52441 agaattaatg aaatgggatt aagagaagaa gattattggt ggtatttaga acttagaaag
    52501 tttggtactg ctactcactc tggatttggt cttggatttg aaagaataat tatgtatatg
    52561 acaggtatgt ctaacataag agacgttata cctttcccaa gaactcctaa aaatgctgaa
    52621 ttttaataat tatatataat aaaacttata taactttaag tataaacact aaaatgactt
    52681 gcatatttat ctattttaga gatatgcaag ttatttttat tgtactataa atatataatt
    52741 tgattacttt tattttgcga tataatactt ataggtttat gtaatagtat gtaacctttt
    52801 aataaaatgc aacacaatta cgtccttgtt cttttgcagt atatagggcc ttatcacaat
    52861 tacttataaa ctcatttaat gttattgtct ttccaatttt ctttgtataa actccaatac
    52921 taacagttac acattttaaa ggaacatcat cacgctcaaa attgcaggac tctataattt
    52981 ttcgtaaaca ttctgattta agtttagcat ctgcctcatt acagttaaaa atacatataa
    53041 aaaattcctc accaccatat cgtatagcat gttcattttc agaacttgta aaatctaaaa
    53101 ttactttggc aatattttgt agacaatagt ctcccttttg atgtccataa ttatcattaa
    53161 attgtttaaa ataatctata tctatcatca ttccagttat aggttcttga tttgcatggc
    53221 tgattaattt tttttgtaga atatcttcaa ggtacctacg gttgttcatt ttagtaaggt
    53281 catctgtcat aactttgtcg ttaagtaaat tatttatatg agatatttct tcatattgtt
    53341 gactaattaa aagtttatca tagtaagtaa atacacgatt tctatatagc attgtagaaa
    53401 caaaaatggc aagggatgtt ataaaaaatg tgttgacaat attactaaac tcattgcctg
    53461 gtatagtttg aatcccaggt aagaataaat taagaaacaa aaagcatcct ccaaaaacta
    53521 ttactgattc tccaaatttt aatactgaga aagctgcagc tgctaacata atataactaa
    53581 atacacttag gtcatttcca cctaactggt caagtagagt aattacacaa ccccataggc
    53641 agaaaaaagt cacataaatt atccataaat ttatataaaa cgtacagcaa tccttttttt
    53701 tattccaaag gtaagaaaat acaaacataa agacagaaga tacacaaaat agtgttacat
    53761 acatataaaa ataccattgg cgtcttggtg tgacaaaagg gcctccttct ctagctgaga
    53821 ttgatatcat cataattatt tgagatatta ctattattat gcaaatgatt ttacccgtat
    53881 gataaagata attgcagagt tctttctcaa cattgtcttt gtgttttttg tctagaggaa
    53941 atccaaaaaa tttttttatt ttttctaagt tcatataagc ctccattaaa aataaagcat
    54001 aaaaataagt attcctttct atcattagat atttggaata cttcgtttat gatttaatta
    54061 tatcaaaata tattgtgtgt aacaatctaa aattgtgata gataaaattt attagataat
    54121 gtcgagtaac tttattgatt aatactttat tataattaag atttacagat agtttactac
    54181 tgaaaataaa gaatagaata aatttaaatt aagcataata aaatataaat aataaagttc
    54241 gcattaacaa actatttaaa tatcaagacc tgttaaaaaa caacagcaat aaacaaataa
    54301 aatagataaa tatatccaat taaggtataa ttaagtttta tatgatatag ttatatttga
    54361 taatttacaa tagtaaaact tggagggaaa aaatgggtga aaagaaaata aataatatga
    54421 attattcaca taataattat tccaaaaaga caggtgaacc aactagaaaa ataaaaaata
    54481 gccatatcac tcttaaaagt aaagaagaaa aaatttgttc tagttgcaat aaagaaaata
    54541 atactaagga taattactgt aaattttgtg gaaatgaact ttatgaaata gcatcattaa
    54601 gaccacttga aacaaaacta gacttgaaat ctaaaataaa agagctttca tatcatgcaa
    54661 ataaaagagg tgtattttta acaacattta caaccatatt tatattgttt attattgcat
    54721 tgatatttaa agctattata actattcaat ttaatgatat tagttattta ataaatccag
    54781 cacatataat cctagctctc aacttgggtc aaattagtgt atctatgtct accatgatgg
    54841 gttctgggtt tataaatgcc aatataggtt tgttgatatt acttataatc cctattttgg
    54901 tattattaat atcaaaccta atttttatga gaaaaagatg tagagattca aaaactgtat
    54961 tagcaaattc tctaggagtt ggaatttttt atggattagt tttagctatt ttatctattt
    55021 ttactaatgt aaaaactagt tcacatagta tgttagaata tggatatgca ttagaatata
    55081 gttatgaatt ttttagtgtt cttttaaatg gatttgtatt agggtttata tgtacataca
    55141 taactactta taaaaaaagt tatgaaaagg aaaatatgta tttatctctt ttttcaaatt
    55201 ctataagaac attcatatta ggttatgttt tagtattagt aatactattg gtactaacta
    55261 tatctgatag tagttactta aatgaactag atatgagtag ttattcaaat ggattaaatt
    55321 tatttactat actcccacaa atagctagtt atatgtgggc ttttgcaaat ggaatttcag
    55381 taaccataat aaactcaaca gtatcaatgt ttactttaag ttcttcatct ttatttggag
    55441 atactaaatt aatgttttat gcaatgggag cattgtctat gttaatatta ttattaaatg
    55501 gatacaagct aagatttaag tataatacag atagtataag acctataata gtatttagta
    55561 tatattatgc atttttgatg ggaatattgg ctttatttag tacatttata ctagatagta
    55621 atattaattt ctttaataca actaattatg gaacaacttt aataatgcaa tttaaagtct
    55681 tacaagctat tgtaatttca tttgtatata gttttgtgat atctttaata ggatataagc
    55741 taaattcagc tgattaaata tgattggagg gaatttctgt ggaaggaaat actaataata
    55801 aagtagatga taaattttct aaggttaaga aaaagtttgg tagtggaaaa tttaaagttg
    55861 aattatataa agaaaaatca aaattaaata gagagattga tgatattcaa gaaaagaaag
    55921 caaagatatt cttggaaatg ggaatactga catatcaaaa aattagagaa gattctatta
    55981 atgataatag ttttgataaa ttctgtgatg agttactaga acttgataag ttaatttatg
    56041 aaaaaaatat ggaaattaat gaaatggaaa tggcagaaag taatgtaact tgtgagtgtg
    56101 gttatgttgg aaatgtaaat gataaatatt gtgctgaatg tggtaaaaaa tttgaattgg
    56161 aaaatgaatg tgatgatttt ataatttgtg gttattgtga aagtgagata gatacagagg
    56221 ctgaattttg tccatgctgt ggaagaaaaa ttataatgga tttagaataa tattgggaaa
    56281 cctatgtata gtatacatgg gtttttttat tgaaattatt taaatggact tataacagta
    56341 gttatataaa atgaaaataa acaccaaaaa aatgaaataa aatttctttt aaatgtaaaa
    56401 aacaagctaa tatataaaat aaatcagtaa aaatatagat gcgagaaaaa aatatgaaaa
    56461 aatattgcaa agaaaaagtt ttcatgttat tattaataca taaatcaaga aaataaattt
    56521 cagaaacgag gtaatttaaa aaatgctaga taaggtaaag ggaagattaa tagtatcatg
    56581 ccaagcttta gaagatgaac ctcttcatag tccttttata atggggagaa tggctaaggc
    56641 tgctatggaa ggtggagctg ttggaattag agctcaagga gttgaagata ttattgaaat
    56701 aaaaaaagtt actggacttc ctgttatagg tattataaag agaaactatg aagattcaga
    56761 tatatatata acgccaacta aaaaagaagt tgatgaactt ttaacaacag ggtgtgaaat
    56821 gatagcatta gatgctacca atagagttag accaaataat gaagacttaa aagaacttat
    56881 aaagtatata aaagaaaacg gtgttctagt aatggcagat atttcaaatt atgatgaggc
    56941 tatcaaagca caagagtacg gtgtagattg cgtttcaact acactatctg gatatactcc
    57001 atatactaaa acattagaag gtccagattt tgtattaatg gaaagattag ttaaagattt
    57061 agaaatacct gtaatagctg aaggaaaggt aaatactcct caagatttaa aaaaggtctt
    57121 tgagttgggt gttcattcat cagtagtagg ttcagcaata acaagaccac aattaataac
    57181 agaaaaattt gttaaagcaa ttgaaattaa ttaaaaatta atttataaat taaaaaatta
    57241 ttatatttaa ctatatttga aaattaaaat tggaggtaaa gatatgagaa caacagatat
    57301 gaagggaatt tattcagcat tattagtttc atttgataag gaaggaaata taaatgaaaa
    57361 agggttaaga caaataatta gacacaatat agatgtttgt aaagtagatg gtctttatgt
    57421 aggaggaagt acaggggaaa acttcatgct ttcaactgat gaaaagaaaa gaatatttga
    57481 aatagctaaa gatgaagtga aagaagaaat aaaattaata gctcaagttg gttcagttaa
    57541 tttaaaagaa gctgtagaac ttgctaaatt tacaactgat ttaggatatg atgcaataag
    57601 tgctgttaca cctttttact ataaatttga ttttgaagaa ataaaacact attataatac
    57661 aataataaat tctgtagata atagattaat aatatactct ataccatttt taactggtgt
    57721 tgatatgagt ttagaccagt ttggagaatt atttgaaaat gaaaaaataa taggtgttaa
    57781 gtttacagca gcagatttct acttattaga gagaatgaga aaaacattcc caaataaatt
    57841 aatatttgca ggatttgatg aaatgatgtt accagctaca gttttaggtg tagatggggc
    57901 aattggttct acattcaatg taaacggtgt cagagctaga caaatattcg aattaactaa
    57961 gaatgaaaaa atatcagaag cacttgaagt acaacatgtg actaatgatt taataacaga
    58021 tatattagga aatggcttat atcaaacaat taaattacta cttgaagaac aaggtgttga
    58081 ggctggatac tgtagacaac ctatgaaaga agcaactgat gaaatgaaat caagagcaaa
    58141 agaaatatac agaaagtatt tttaataaaa tacttttagt atgggaggat tagtcaaatg
    58201 gtaggattta cttggataga ttttgcaata ctagtggttt acttattggc agttttattt
    58261 gcaggattgt tattttcaaa aaaggaaatg aagggaaaag agttcttcaa aggtgatgga
    58321 actattccgt ggtgggttac atcagtatca atatttgcca cattattaag tccaatatca
    58381 tttttatcat tagcaggaaa ttcttatggt ggtacttgga ttttgtggtt tgcacaatta
    58441 ggtatgttta tagcaatacc tttgacaata aaatttttct tacctctata tagtaggtta
    58501 gaaatagata cagcatatga atacttagaa atgagatacg aaagcaaagg tcttagagta
    58561 cttggagctt tgatgtttat aatatatcaa ataggacgta tgtctatcat aatgtatctt
    58621 ccatcaatag ttctggctgg tcttacagga atacctgtaa atgtactgat aatagttatg
    58681 ggtgtaatag caataattta ctcatataca ggtggattaa aggctgtctt gtggaccgat
    58741 tttatacaag gaatggtatt aatagtaggt gttacaggga cattgttcta tttaatagca
    58801 agtataaatg gtggttttgg aacagtaatg gatactctta caactggaca caagttctta
    58861 gcaagtaatg aagttatgtt tgataaaaat atactatcta caagtgcatt tataatattt
    58921 gttggagctg gtcttaatac tttttcatca tatgtatcga gtcaagacat agttcaaaga
    58981 tttactacaa caactgatat aaagcaactt aataaaatga catatggaaa tggtgtttta
    59041 tcaatgggac ttgcaacagt attttatcta attggaactt gtttatttat atactatact
    59101 caaaacccag atttagctca aacagttcaa caagaccaag tatttgcatc atatatagct
    59161 tatgaattac cagtgggtat aacaggaata ctacttgcag cgatatatgc agcttctcaa
    59221 tcaactttat cgacagggct taattcagtt gctacaagtt ggactttaga tatacaaact
    59281 atcataacaa aagatataag tatggaaaag caaactaaga tagcacaata tatatcatta
    59341 ggtgtaggta tactttctat agctgttgca atagtattag caaatggaga aataaagtca
    59401 gcatatgaat ggtttaatag ttttatgggg ctagtactag gtgttttagc aggaatattt
    59461 gtacttggtg cattttgtaa aaaagcgact aaaatgggag catatgttgg attcatagta
    59521 tcagcaattt tagttgttta tttaaaatac cgtatgccag aagtaacttc ttgggcatat
    59581 tctctaataa caatcacgac ttctgtagtg gtaggtacaa tagttagtgc aatagaagct
    59641 aaagttaaag caaaagtatc tttaccaaaa gctgagacta ctgtttatta tgaaaaataa
    59701 tatttaaatg ataaattgtc ttatagagtg attagataaa taatttagtt actctataag
    59761 attgtataat atactttgta tataaaagta taaaaataga ctttataatt atatagggag
    59821 atagtgctta tgatatttgg aaatataaat catagtaaga catattcaac attgcatgaa
    59881 gatttactta gatgttttga atatgctaaa gataattcat taatagatta tgaaaaaggt
    59941 acttatacaa tagatggtga tgatattttt gtgaatattg tagagtataa aacttgtgaa
    60001 aaagaagata gattctggga agctcataag aagtatatag atgttcattt gatgcttgat
    60061 ggatgtgaac gaatagatat aaattttata gaaaatttag aacaaaaaat atatgaagaa
    60121 aagggcgatt tcttgcctct tgatggtaaa aataatggat acatggaact tagaaaaggt
    60181 gattttttag tttgttatcc ggaagatgct catatgacag gaataaaagt tttagaaaaa
    60241 gaaaatatca aaaaagctat ttttaaggtt ttggttagat aatgataatt tatataacca
    60301 ctagggctga aaatagtgat ttaactttca gcgattagtg agacttcgta aattttagtt
    60361 agattactat tttcagcctt agttttttat gagaaaattg aaaataatta aaagcttaaa
    60421 attttgtaaa ggagttaact tatggaaaat atagatgtat tcttattaat aggacaaagc
    60481 aatgctagag gattgggaaa tccaaaagag agcatcattc caaatgaaaa ttgttttgaa
    60541 tatttgagta ctgatgaaat tataaatatg agatgtgagc tagagacttc agaaggtgat
    60601 ggtactatag cacctgcatt ttcaaatgaa tggaataagc ttactggaaa taaagtttgt
    60661 tttattcaca atgcaaaaga tggttcaaga ataaaaaact ggaatcatga taataactgg
    60721 tttctaaatg acactataga aaaatttaat acaggctgta ctaccttaag taaacattat
    60781 aatatagaaa ataaatacgt tatttggatt caaggtgaaa gtgatgcaaa atatggtagt
    60841 gatgtattgt actataaaga aagtctaaaa aaaattgcgt ataggcttaa agaagaatgt
    60901 atgattgata aaatgtttgt aagtttaact ggttattggc ttggagaaga tgaatatttt
    60961 attagaacaa gacgaattgc agcagcacag gagtcggctt gtagtgaatg tgatatatta
    61021 tgtgtaggaa gcaaaattgc tatgcagttt catgacagag gattaactat tgatgatgtt
    61081 cattacactc aagaaggtct taatatgtta ggagaggact tatgtaaaaa tatttataag
    61141 tatcatataa ccaaagaaaa aagtgtatta agtgatacta tagacttatc agaagcaagg
    61201 aaatatatct gtgaattgga aaagataagt aaaagatttg tgtaatataa aattttaaat
    61261 agagttgtta atttataaaa ataatttgta tggagatatg ttatgaaaaa ttacgtttgt
    61321 atagatgttg ggggtacttc aataaaatat ggttttatta gagaagatgg atttataatt
    61381 gataaatcaa gtcttgatac tgaagcaaag gaaaaaggtg gagaaggaat attagccaaa
    61441 ataaaagata tagtaaaaaa atatatagag gaaaatgaaa taagtggtat atgtatatca
    61501 actgcaggta tggttgaccc agttgaaggt aaaatactat ttgcattaga agaattaatt
    61561 ccaaactata agggcatgca attaaaaaaa gaagtagaaa aagagtttaa tataaaatgt
    61621 gaagttgaaa atgatgttaa ttgtgctgga cttggtgaaa tgtggcttgg agcaggtaaa
    61681 gatgcaacat cttctatatg tttaacaata ggtactggaa ttggtggatg tattataata
    61741 aataataaac tgataaatgg gtttagtaat agtgctggtg aagtaggata tatgaatgta
    61801 aatggaagtt cttttcaaga attggcatct actagcagtc taattaaaaa agtagcaaaa
    61861 ataaaaaatt tgaatgaaaa tgacttaaat ggaaaaataa tttttgattt agcaaagaat
    61921 aatgatgaag attgtttaaa agaactcgat aatatgataa aatcattagc agttggaatc
    61981 gcaaatatat gttatgtaat aaatccagaa gtggtaattt taggtggtgg gattatggca
    62041 caagaaaagt tcctaaaacc aaaaatagac aaagcattaa aggaggttct tatagagaga
    62101 gtttacaaaa acactaatat agaatttgca aagagacaaa atgatgcagg aatgcttgga
    62161 gcattatata attttttaaa caaaatataa agaatagaag ggtttttaat gagtatttta
    62221 gaacaattgg aatcaccaac ttttaaagtc acaaaatcag ataaattatt aatagcttat
    62281 ataaaagaaa atatagatga tgtattttat aaaccaatat cacaaatagc taaagagagc
    62341 aatattggtg aagcaactat aactaggttt gtaaaaaaaa tgaattttaa tggattacag
    62401 gattttaaag ttactttagc acaagaaata tcaactatca ataaaaaaaa tattataaat
    62461 aaaaatatac aaaatgatga gccagctttg gatacagcaa aaaaactttt aagttcaaat
    62521 gtaaccactt tagagaacac agtagagata atcaatagta aggatgtaca tgactgcgca
    62581 aggcttataa taaatgcaaa gaaagtttac ttcataggta ttggatattc tggtataata
    62641 gcacaggatt caaactataa atttatgaga ataggtctta attgtgtaag ttttgacagt
    62701 agtcatacaa tgataatgat gtcttccata atggaagaag gagatttaat aattgcaatt
    62761 tcacattctg gtgaaacaga agagattata aaaactgtaa agcttgcaag agccaataat
    62821 gctaaaatta tatcaattac tgaaaataaa aattctgaat taaaggatat ttcagatgta
    62881 cacttatctt atgtatcggg agaaacagta ctggaaactg gttcaatatc ttctaaatta
    62941 gcacaatttt tcattattga tttagtatat acacaggtag taaaagaact ttctaatgaa
    63001 gctattgaaa ggaaaattaa aactacaaat gctataaaat tgtttaaaaa tgaataaaat
    63061 aagaaatttg taatgtttta tagagctctc ttaaaaattt aggaatggag agatattaca
    63121 tgtctaaatt aaaagattat aatattgata aagtaataat ttttaataat ttaagtacta
    63181 aaacgaagaa tcaacttaaa gaaaaagtta aactaaaaag attacaaaaa aaagaaattt
    63241 tattttatga aaaagatagc ttagacagtg tttatatact tttagaagga aaggtatcta
    63301 tttttaagat aagtgaaaat ggagagagaa aagtaatttt catattaaat agtggggaag
    63361 tcataaatga tattacactt gacgcttcta aaaactctac tgttggctgt gaagcatttg
    63421 aaaattcaat tgtagcttat tatagtattg aagaattttt agaaattatg caaaatgatt
    63481 ttcaacttac aaaggatatt atttcttata tggaaagaag aataagacgt ttatatagac
    63541 agttgaaaaa ttctatatcc ataaaagtag ataaaaaact tgcagcaaaa ttatataggt
    63601 taagtagaga atttggagtt tgctgtggag aatggacttt actaaatgta aatattacag
    63661 ttacatattt agctgatatg cttggctgta aaagggagac tgtttccaga atgataaagg
    63721 tattgcaaaa agaagaactt attaaaattg acaataaagg tttttatgtt aaagaaactg
    63781 aattatcaag gtattttaaa aataattaat tatgattaag taagagtatt attagaattt
    63841 taattaaata aaaatattat
//
LOCUS       DAMJYX010000012        62451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJYX010000012 DAMJYX010000000
VERSION     DAMJYX010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.012"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..591)
                     /locus_tag="PC479_000819"
     CDS             complement(<1..591)
                     /locus_tag="PC479_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2803402.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC479_000820"
     CDS             complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="PC479_000820"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2803403.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2758..3297)
                     /locus_tag="PC479_000821"
                     /pseudo
     CDS             complement(2758..3297)
                     /locus_tag="PC479_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3374..5179)
                     /locus_tag="PC479_000822"
     CDS             complement(3374..5179)
                     /locus_tag="PC479_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803404.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5575..5952)
                     /locus_tag="PC479_000823"
     CDS             complement(5575..5952)
                     /locus_tag="PC479_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2803405.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6857..7342
                     /locus_tag="PC479_000824"
     CDS             6857..7342
                     /locus_tag="PC479_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803406.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7542..8081)
                     /locus_tag="PC479_000825"
     CDS             complement(7542..8081)
                     /locus_tag="PC479_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2803407.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8074..8487)
                     /locus_tag="PC479_000826"
     CDS             complement(8074..8487)
                     /locus_tag="PC479_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2803408.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8523..9644)
                     /locus_tag="PC479_000827"
     CDS             complement(8523..9644)
                     /locus_tag="PC479_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2803409.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10232..11884
                     /locus_tag="PC479_000828"
     CDS             10232..11884
                     /locus_tag="PC479_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2803410.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12183..12932
                     /locus_tag="PC479_000829"
     CDS             12183..12932
                     /locus_tag="PC479_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803411.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12945..14972
                     /locus_tag="PC479_000830"
     CDS             12945..14972
                     /locus_tag="PC479_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803412.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVNQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            15116..16699
                     /locus_tag="PC479_000831"
     CDS             15116..16699
                     /locus_tag="PC479_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803413.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16671..17291
                     /locus_tag="PC479_000832"
     CDS             16671..17291
                     /locus_tag="PC479_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803414.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17480..18406)
                     /locus_tag="PC479_000833"
     CDS             complement(17480..18406)
                     /locus_tag="PC479_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2803415.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            18883..21927
                     /locus_tag="PC479_000834"
     CDS             18883..21927
                     /locus_tag="PC479_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2803416.1"
                     /translation="MKKMLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKQRVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSIEYVKQYTDSHSGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDDNG
                     NYSVAGATYGVFADKDCTKQLATLTTDENGNTDVVEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGRTATLKVSDTPKVTDTLIKLFKIDMETQKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIH
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTYGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGENHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSKSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     FSNPNEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKKVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIDDEGQTITFKEKPEEPEKPKTPQTPEKPS
                     RPSDSPKTGDSTNVMAFIVMLLVSAGGLAGTYLYKRRKMKKS"
     gene            22166..22492
                     /locus_tag="PC479_000835"
     CDS             22166..22492
                     /locus_tag="PC479_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2803417.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVELVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVQEK"
     gene            22508..22891
                     /locus_tag="PC479_000836"
     CDS             22508..22891
                     /locus_tag="PC479_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2803418.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQPKK"
     gene            22993..23430
                     /locus_tag="PC479_000837"
     CDS             22993..23430
                     /locus_tag="PC479_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803419.1"
                     /translation="MCMECYVDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPK
                     RGLQRWNFPFKSLEIAKLYLRTADYSTKKSCGIYELKSENGRLSYKIFADSEDLLLYL
                     KKNKGKTCKDMKPVFMIEEYREYANTQIRKLTSDEIQKYMSER"
     gene            23523..24917
                     /locus_tag="PC479_000838"
     CDS             23523..24917
                     /locus_tag="PC479_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803420.1"
                     /translation="MRMILNKGHRIRASNKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDNFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYMEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNSDLADLGTVMPNVY
                     HIKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVNLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            25100..26296
                     /locus_tag="PC479_000839"
     CDS             25100..26296
                     /locus_tag="PC479_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2803421.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLNTLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGRYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            26309..26443
                     /locus_tag="PC479_000840"
     CDS             26309..26443
                     /locus_tag="PC479_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2803422.1"
                     /translation="MWILLKDFLLVSMGMGIGVVLMCILNVGKEADYEMKQLEESEDN
                     "
     gene            26444..26665
                     /locus_tag="PC479_000841"
     CDS             26444..26665
                     /locus_tag="PC479_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803423.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            26700..27341
                     /locus_tag="PC479_000842"
     CDS             26700..27341
                     /locus_tag="PC479_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2803424.1"
                     /translation="MWIYKILIYGGVILKKAMIYVHGKGGSYLETEQFKNICLDFDVF
                     GVDYNDYFPWIVKNKIMEVYDKISEKYDYIYVLANSIGAYFTMHTLQNFKIEKALFIS
                     PILDMEQLIVDMMSWANVSEKELSERKEIPTDFGETLSWEYLCFVRANPINWKTPTEI
                     LYAEHDNLTSRQTVDKFIDRHCANLTVMNDGEHWFHTDEQILFRDNWLRKVIQ"
     gene            27542..28168
                     /locus_tag="PC479_000843"
     CDS             27542..28168
                     /locus_tag="PC479_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2803425.1"
                     /translation="MKYIMLHGLGQSSESWSDTVKVFNDDFEVLCPNLSDWLSGKTPC
                     YDTLYRTLETYCEQFEEPLNICGLSLGGILAMQYAIEHPEKMNSLVLIGTQYEMPKYL
                     LKMQNLIFRIMPCSAFEKMGFQKTDFINLCKSMTELNFKHELKKISCSVLVVCGDKDK
                     INKKASLELQQQIENAEIAIIENAGHEVNTDNPTLLGQKLNVFFKQYV"
     gene            28257..28553
                     /locus_tag="PC479_000844"
     CDS             28257..28553
                     /locus_tag="PC479_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2803426.1"
                     /translation="MSDIFKDMQANVGCEYISDLPSYKRKVWQEMKRLNPANYEERQL
                     EDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLTCR"
     gene            28472..28975
                     /locus_tag="PC479_000845"
     CDS             28472..28975
                     /locus_tag="PC479_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2803427.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGIGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPNCKSMIDVAKQKLMNDPTFKHLSEDCQAYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            28993..29496
                     /locus_tag="PC479_000846"
     CDS             28993..29496
                     /locus_tag="PC479_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2803428.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIGEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNSMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            29615..30013
                     /locus_tag="PC479_000847"
     CDS             29615..30013
                     /locus_tag="PC479_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2803429.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            29991..32441
                     /locus_tag="PC479_000848"
     CDS             29991..32441
                     /locus_tag="PC479_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803430.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGNLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEYQLPKKKLQKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSDSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDLGEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            32441..34627
                     /locus_tag="PC479_000849"
     CDS             32441..34627
                     /locus_tag="PC479_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2803431.1"
                     /translation="MKERIKGAFTKKKIFHFLKMSLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLFMRHRARCMERRLAGAVTAGGVAGAVAGRTISNRKPTRNTATKDSRGNTTSSMGQR
                     AGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTVYAVYSAKEKAKSSVSDFKRGMVQE
                     QQSRQTGRLEKQEQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     KPSAEKMQEVKRPATATTSKASEPVKTNVIKERPLFSGASDKKATQSAQPVHRQNVEK
                     VVSQETRQNYTKDRRTKVQQTQTVQKNQQTTEKTPNLVMKKGQKKK"
     gene            34624..35631
                     /locus_tag="PC479_000850"
     CDS             34624..35631
                     /locus_tag="PC479_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2803432.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHAFNLA
                     ENFAREKSGGKKVIYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVSQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGILVSPTQMYHAGNPIGYADLSSSYWQQHLIGAGRVK
                     Q"
     gene            35647..36558
                     /locus_tag="PC479_000851"
     CDS             35647..36558
                     /locus_tag="PC479_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2803433.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKAVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIESRTQA
                     INGYLTKELQDLNVDTIRTDIPTSSTVTDVIVWSIEQSGTDTFSATYEVDQQIKEGEK
                     TSNVKATYTVKVHIDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATA
                     FLETFFKLYPTATEKELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGDNVKVKVAVKF
                     LDNQAKATQVSQYELVLQKDSNWKIVG"
     gene            36703..36924
                     /locus_tag="PC479_000852"
     CDS             36703..36924
                     /locus_tag="PC479_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2803434.1"
                     /translation="MKKGQHIFGTVKVGERGQIVIPKEAREIFDINAGDTLLVLGDEE
                     QGIAIVKADVMKELAVKILKGLGNLCLDN"
     gene            36953..37570
                     /locus_tag="PC479_000853"
     CDS             36953..37570
                     /locus_tag="PC479_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF2803435.1"
                     /translation="MKNSISLFLPLALLLDLTIPFFLAPFYKGYSHSMQVMSVLGNNK
                     SPLHFIYNLWLVLCGIILLMSGFKIYEIVSQKSKNIAVILFIVILIYAIGGCILSGIF
                     SVGETKSLNTIPEKIHGYGAVIGFMCLTFAPLLISLYWFRIKEIPLALFSMICFLLAF
                     IFFTIFVMADKPNYKDTIIAFEGLWQRLSLLFMYLPLGCLILLEK"
     gene            37587..37823
                     /locus_tag="PC479_000854"
     CDS             37587..37823
                     /locus_tag="PC479_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803436.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGVSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPAPEEYIYIITKKTVKGLKV"
     gene            37820..38792
                     /locus_tag="PC479_000855"
                     /pseudo
     CDS             37820..38792
                     /locus_tag="PC479_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            38805..39419
                     /locus_tag="PC479_000856"
     CDS             38805..39419
                     /locus_tag="PC479_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2803437.1"
                     /translation="MKKVYVYLLDDMAECEIGYILQAFSMEKLLKNGTKEFEVKTVGF
                     NKEPVLTLGGLTIVPDYSISEVDFNASTALLLPGSSTWGSKENQEILEKAQECLNKNI
                     LVGAICGATLALADYGILDKFKHTSNSLEYLNFFSRNYAGQSLYVCSNSVLDRNLVTA
                     NSAGSLEWTKNILNYLNVYSDNKITAFYNYYSTGNAKYYDELLK"
     gene            39447..39620
                     /locus_tag="PC479_000857"
     CDS             39447..39620
                     /locus_tag="PC479_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF2803438.1"
                     /translation="MGIMPICNNKTRTKIRVDTELISFPLFCPKCKHETLINIKHGNV
                     TTIKEPDAKTQSR"
     gene            complement(39852..40103)
                     /locus_tag="PC479_000858"
     CDS             complement(39852..40103)
                     /locus_tag="PC479_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2803439.1"
                     /translation="MKLFIGDIIQKLRKIKGLTQEQVANALGITTSSVSKWETNNTYP
                     DISLLSPLARLLGTAVDNVFNFKQKLSEEEIDTLLNRSK"
     gene            40368..41072
                     /locus_tag="PC479_000859"
     CDS             40368..41072
                     /locus_tag="PC479_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2803440.1"
                     /translation="MMKAILKTNNLCKDFKKQKAVKNVSITVRENSIYGLLGPNGAGK
                     STTLKMITGMLRPTSGKVLFNGHEWNRKDLEQIGALIETPPLYENLSAVENLEVRAKL
                     LNIPKTRIDKVLKMVDLQNTGRKKAGQFSMGMKQRLGIAIALLNSPKLLILDEPTNGL
                     DPIGIQELRSLIRSFPSKGITVILSSHILSEVQLIADDIGIISNGILGYEGQMNKDEN
                     LENLFVEVVRKSQEER"
     gene            41074..41817
                     /locus_tag="PC479_000860"
     CDS             41074..41817
                     /locus_tag="PC479_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2803441.1"
                     /translation="MKNYISSEYLKHKNTFAKKLVWLAPILTLCLNILAPMWYQQNSY
                     NWWYVFLYPGCLTLLSILIIQRDNGKLKFRAILSLPVDLKKVWYAKIITCIIYIVLAN
                     TILMLCNVAGGFIIQHIFDIPMTISPMQALFGTLCIILASIWNIPLCLWFTKKVGVFA
                     TLILNVGMSFMLGTLGANTSFWFICPYSWVTRLMVPTLGILPNGESVTGSALSTPLPL
                     VIMTICLSLLLLYTISKSTAKSFIKQEVL"
     gene            41817..42560
                     /locus_tag="PC479_000861"
     CDS             41817..42560
                     /locus_tag="PC479_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2803442.1"
                     /translation="MFSIIRCFQAEWLKSRHTPLLLVHVLFPLIGAGVFAGYFHISGW
                     SEMTNVTTFLEVLAIIFPFVIGIVVGMIVELENGAGHFQLILGTIRSRIAVYIGKLVY
                     LIVLATFATILCIFLFAVLYPVMPFSFYIKPLIMLILSVIPLYLISIPVGFSLGKSVS
                     MGIGIVGSLLSALLVTGLGDFIWKFLPWGWGVRFMDYCVLRYVNEGQFHSNLPEFQLG
                     FILMLVFTVVLFIISLVWFQRWEGVKENE"
     gene            42553..43362
                     /locus_tag="PC479_000862"
     CDS             42553..43362
                     /locus_tag="PC479_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HDF2803443.1"
                     /translation="MSKKMMILLFAVIICLSLAGCSLQDKIGDYSREQCYLNKENVTQ
                     FSYKGNDYTILADTVSNSGLGEWIGYIRQLAAVDESGKILLQENLKTATFQTLADLAD
                     LVDKAPNDAYIIPFLNVYAAPNADDYLIVDINGGYHKAVIDENIKGTDTVFDFKDIEQ
                     SMSGKFEINPQNATQLLCDGTVYQVTSDTVSNNELGSYIGILAENVIFNAETKIPLSK
                     EELRKIDWYGENAGQHREQWIYKDIYEIHGTEKTEAVAVQINDRYYIAKRQ"
     gene            43425..44084
                     /locus_tag="PC479_000863"
     CDS             43425..44084
                     /locus_tag="PC479_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803444.1"
                     /translation="MATILMIDDERPILELVKNGLQKDGHLVTVYTSAAQVPLDKLNR
                     YDLIILDIMMPDIDGFSYCDKIRSLVDCPILFLTAKTMENDITFGLGLGADDYLTKPF
                     RITELRARVNAHLRREHRERHIALTFDRIKIDLSAKELRVDNIPVPLTKSEYLICEYL
                     ASNKGQVFSKEQIYEATFSLYGESDSSTISTHIKNIRSKFEKTGVQPIVTVWGIGYRW
                     E"
     gene            44075..45439
                     /locus_tag="PC479_000864"
     CDS             44075..45439
                     /locus_tag="PC479_000864"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012722.1,HMM:NF014567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2803445.1"
                     /translation="MGIKMKTSLTRTFYKFLVCLLGGLCLSVVVPFACMMFASSIGLV
                     TLADNNERQTKALVPILTATPNVSDIHFPTGTKFLRLTKNYGYIDTNMSEEEKEKALM
                     FAKTGFMDTSGKTQFIFITRDDEYIVLQYVIGSQYLNAQFNQYLPSPEIMMISLITIS
                     SLGVCVYLTVHFAKELRKELTPILEATKEIENNNLDFTIGHSKILEFENVIISFDSMR
                     NSLKGSLEKQWRSEKAQREQIASLAHDLKTPLTVMQGNIDLLDETELDEEQKLYLSYA
                     MSSSEQMKQYIKILIDISKASAGYQLQMEDVNFPEFWKHVLSQTEIICKDKGIVIQQT
                     QHNFPQNITGDRMLLERALMNLVSNSLEYSPENSTLYIDVDSKDNYLNICITDCGCGF
                     SQEALDHAKERFYMADQSRSSKLHYGMGLYIVDIIIKQHKGKLLLDNSTETKGAKVTI
                     KLPL"
     gene            45801..46220
                     /locus_tag="PC479_000865"
     CDS             45801..46220
                     /locus_tag="PC479_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2803446.1"
                     /translation="MKLSDFQKTIQCQFECKLKKVTKGIVRNYQKELKRRKNKEIPFC
                     ELPELVIEKLSVLDDYDSDYTLFNVCGNDISILDDELAEALKKLSDRNRENLLMYYFL
                     EMSDTEIAKLQNISRSGVFQNRYNSLELMKKILKGEK"
     gene            46224..46472
                     /locus_tag="PC479_000866"
     CDS             46224..46472
                     /locus_tag="PC479_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2803447.1"
                     /translation="MKHIFKKPSFYLISSATDGDEKAIEKILAIYDPYISKCCLRSLY
                     DEYGNVYIAVDMELKGRIREALIKMILDFDIPLETEEE"
     gene            46848..47051
                     /locus_tag="PC479_000867"
     CDS             46848..47051
                     /locus_tag="PC479_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2803448.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            47130..48320
                     /locus_tag="PC479_000868"
     CDS             47130..48320
                     /locus_tag="PC479_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2803449.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGIDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRQYLMNALKNDLLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEKHLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEIGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILKNRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            48940..49860
                     /locus_tag="PC479_000869"
     CDS             48940..49860
                     /locus_tag="PC479_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2803450.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            49946..52366
                     /locus_tag="PC479_000870"
     CDS             49946..52366
                     /locus_tag="PC479_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2803451.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            52366..53388
                     /locus_tag="PC479_000871"
     CDS             52366..53388
                     /locus_tag="PC479_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2803452.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            53503..54249
                     /locus_tag="PC479_000872"
     CDS             53503..54249
                     /locus_tag="PC479_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2803453.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            54335..55585
                     /gene="cme"
                     /locus_tag="PC479_000873"
     CDS             54335..55585
                     /gene="cme"
                     /locus_tag="PC479_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2803454.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(56102..57115)
                     /locus_tag="PC479_000874"
     CDS             complement(56102..57115)
                     /locus_tag="PC479_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803455.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(57118..58146)
                     /locus_tag="PC479_000875"
     CDS             complement(57118..58146)
                     /locus_tag="PC479_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803456.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(58146..58991)
                     /locus_tag="PC479_000876"
     CDS             complement(58146..58991)
                     /locus_tag="PC479_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803457.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            complement(59411..60541)
                     /locus_tag="PC479_000877"
     CDS             complement(59411..60541)
                     /locus_tag="PC479_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2803458.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            complement(60538..61209)
                     /locus_tag="PC479_000878"
     CDS             complement(60538..61209)
                     /locus_tag="PC479_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803459.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            complement(61423..62073)
                     /locus_tag="PC479_000879"
     CDS             complement(61423..62073)
                     /locus_tag="PC479_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2803460.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
BASE COUNT        21327 a        10280 c        10480 g        20364 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcatttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt ttattttgaa tttttcattt gctttacttt
      781 tttatttgct ttactttttc atttatttag atttttcatt tgttttagtt tttattttat
      841 ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta tttgttaaga
      901 ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac tatatctcta
      961 tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga gttctcatct
     1021 agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga aactaatgct
     1081 ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct tagagggttt
     1141 cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa tgctgatgaa
     1201 taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag ccctgccatt
     1261 atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc tgctgattta
     1321 tagtaacagt cagcctcatc agcctctact actactgtta ttgggcattc atcatatatt
     1381 gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg aacaaatacg
     1441 tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt agccatagtt
     1501 ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc tgtatcttga
     1561 accattactc catggtcatc agcttctgca taatcattag ctaatcttac tgcatcatca
     1621 tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt taatctaaat
     1681 tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt agctgtccaa
     1741 gcaactcctc taccatgatt tccatctgta gctgtaaaga aagttatttc tcctaatttt
     1801 tcttttattt cttttgaagt taatttatca taccctaact cagatatatc tgtatctaat
     1861 ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa tgcgtttaaa
     1921 ccaaatctat aagattcgtc tttaacatat attccgctta cgcctagtga ttttgctaag
     1981 ttatctaatt taactaaagg agttttttca tattgtggaa agctttcatg aaaagcttta
     2041 gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc ctctgattta
     2101 ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc ctttcaaatt
     2161 tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt accgtgccaa
     2221 tattatttat tctaaaaaca atttttttat gttggaatta catactttta taaaatttct
     2281 ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa attctcatat
     2341 tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt tatcttaatt
     2401 cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata tattactact
     2461 tttattttct ataaatatat ggctataacc ttcttacgca taaactctat aaatttatat
     2521 actttatatt ctttattacc caaatattct ttattaccca aagcctccaa aatggcttaa
     2581 atcttttcat agcttcttac agaagtttac atgggcgtcc ccccaataag caaaagtgca
     2641 atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg caacttcgca
     2701 tttatctgat gaaatgcact atacaaaata ttcataacat ccacaacaga gttaaagtta
     2761 cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa ccaatcctat
     2821 tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac ttgcccgaaa
     2881 aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta cccaacgatt
     2941 ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat ataattctct
     3001 ctgaaactgc tacatactgc tattcattat aggtgataca aacaccgttt tagtatcatt
     3061 aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt
     3121 tagcttgtct atacaacaag cctcatccac atcattgaaa caagccaaag taagatgagg
     3181 tcttgttttc cattctaaat acttcgtact aattttttca tcagcgattt ttttagataa
     3241 atcaaaaagt tgcttttccg tttttttatc ataatacagc tctatcgcat attgcataat
     3301 aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt
     3361 taatattata ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc
     3421 ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact
     3481 tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta
     3541 tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc
     3601 cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat attattccat
     3661 catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg
     3721 gccaattata gttaatcaac atattcatta cattttcttc tattttattt aactgaatac
     3781 ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc
     3841 tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc
     3901 taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta
     3961 tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc
     4021 taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac
     4081 cttcatttgc aagttcaaat ttacctactt tacctccact actagctcca ctaaatgcac
     4141 cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta
     4201 tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct
     4261 ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt gctaatttct
     4321 tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac
     4381 tatttcccca gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa
     4441 atattattat tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta
     4501 tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt
     4561 caacagatac tatatttact atattttcta ttatattttt atctatatct aattttttta
     4621 gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt
     4681 caacattttc aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt
     4741 catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat
     4801 tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata gcaccaatta
     4861 ctcctatagt tcgattatta tactttattg gaactgctat tattacttta tttaggcatt
     4921 tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt
     4981 cctgacctgt ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta
     5041 tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa
     5101 tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt
     5161 cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt
     5221 agctatattt taacatatag tcactcttta aaatatatat aaaacattac ttatttatat
     5281 atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa
     5341 ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat
     5401 ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa
     5461 tatttagata gatttttata tagacttttt taatataaac ttttttaatt gtagactttt
     5521 tttattagtt tttaattttt attagttttg ttattggttt tattgttata atacttataa
     5581 tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca
     5641 tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa
     5701 atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc
     5761 ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga
     5821 tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc
     5881 aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac
     5941 tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc
     6001 ctaaacacca atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa
     6061 tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg
     6121 tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat
     6181 tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc
     6241 tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt
     6301 aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
     6361 gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa
     6421 taaaaccttt acccatacct tatttaccct agttatcata gagcctattg aaaatataac
     6481 agctaaatat aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa
     6541 aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac
     6601 ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa
     6661 tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa
     6721 taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt
     6781 taatagtttt atttaatata cttatattaa ttattttcat atgaaattat tatttatata
     6841 gggggatttt tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt
     6901 agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc
     6961 attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac
     7021 taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa
     7081 agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat
     7141 taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg
     7201 ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt
     7261 tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa
     7321 tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac
     7381 agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa
     7441 tgatatagca caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg
     7501 ataagaatat attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt
     7561 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     7621 ccatatacta tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct
     7681 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     7741 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc
     7801 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc ctcaatcatt
     7861 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7921 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7981 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     8041 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatttt
     8101 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     8161 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     8221 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     8281 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     8341 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     8401 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     8461 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     8521 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     8581 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     8641 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     8701 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     8761 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     8821 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8881 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8941 gcaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     9001 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     9061 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     9121 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     9181 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     9241 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     9301 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     9361 tcattaaaaa tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     9421 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     9481 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccct ttctaaaaaa
     9541 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     9601 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     9661 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     9721 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     9781 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9841 aattctcaaa cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc
     9901 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaagat
     9961 ttagaaatag gtcttgtgtc tattttaaaa tatataaaac agtggtaaag acttatttat
    10021 aagataaaaa atgaatagtg tatacccttt ttaaaaagtt ttttcctttt tatatttgtg
    10081 atacaatgtt aaatataaaa aggaaaattt tgtattaaaa aaatcttatt tgttgatttt
    10141 gtaacttcaa actcagcatc aatttgattt taagattata ttttattaag tatgctaaat
    10201 aacaaatttt taaagattgt gaggtaaaaa tatgaataag aaaaatttag ctatattaat
    10261 atcaggtatt atgttttttt ctagttcttc agcaattttt gcagacaata ctacaaagca
    10321 agaaagatta ataggtaaaa caagatatga aacagcagta gaagtaagta aattgggatg
    10381 ggttcaatca aaaacagcca ttatagttaa tggtaattct attcaaagtg cgctttgtgc
    10441 aaatccattt gctaagctaa agaatgcacc tatactttta gtaaacaaca acagtattga
    10501 aaattcaact aaggctgagc ttaaaaggct tggtgtagat aatgtgtaca ttgtagatag
    10561 tggaaatagc atttcttcta aagtagaaaa tgaaataaaa tcacttaaca taaaaataaa
    10621 taaaatagta ggaaacaata tatatgaaat gtctacaaat gtacttaaag aaatagataa
    10681 gataaaaaag gtagaaaatg ttgcagttgt aaatggacta aagggactat ctgatgcagt
    10741 tagtatagct tctccagcag caatacataa tatgcctata atattagtat cagatgaaaa
    10801 cagcaatgat agtagtataa atttcttaaa caataggtct ataaaaaaat cttacatagt
    10861 tggaggaaat aagattttat cagacaatac agtaaagaaa tatccaaaca gagaaagaat
    10921 cagtggttca gatagaaaca atactaatgc aaaaattatt gataaatttt ataaagataa
    10981 aaaaataaat aatgtatttg tagctaaaga tggtattaac caagaaaatg cattagtaga
    11041 ttcattatca ataggagtac tagctgctaa agaaaattct cctataatga tatctaatgg
    11101 tagccttgta aatagtcaaa aagaatttat taaaaataaa gatatagatg aaataacaca
    11161 agtaggagga ggatataatc aaaaagcatt tgaagatatt ttaaaactaa aaaatgtgaa
    11221 cataagaaat aaaactaaaa aaatatcttt aatacaggct aaagaaatat taaaacaagc
    11281 tcttccagga agcaatattt tagtttgtta ttatgatgaa gatgataatg aatatgactg
    11341 caaagttata aaggataata tcctatatga gatagaaatt gatgcctact ctggtaaaat
    11401 aatagaaatt gaaaaagatg atgacttaaa tgataataat ttaggtacac aaaatatatc
    11461 attggataaa gctaagtcta tagcattaga tgctgttcct aatggtaatt taatctattg
    11521 taaatatgat gctgaagata acgaatatga ggctaaagta ataaaaaata agaaaacata
    11581 tgaattagag ataagtgcat ttgatggaag aattgtagat attgacatgg acaatgattc
    11641 tgatgatgac tttgatgata atgataagaa taactctaat actaatgaca acagttctat
    11701 tattagtgct gaaaaagcta aatcaataat gctagataaa gtgcctggtg ggaaaatagt
    11761 aaaatttaag tatgatgaag atgacaaaga atatgaaggt gaaataataa aaggaaaaac
    11821 tgaatatgag ataacaatta atgcaattac tggtaaaata ttagaatttg aaatagatga
    11881 ttaataaaaa atatatattc tattaattat tacaagcaat aaagccataa atattgaaaa
    11941 tttcaatatt tatggcttta ttttattttt tttaatttaa atccacatta tgccattata
    12001 tatacctatg cactctttta aattaaaatc ttgtccatca tcccaaacct aataaagtta
    12061 gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg tgatttactc
    12121 atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag gagacatata
    12181 caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa gggcaacagc
    12241 cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt ataatgggag
    12301 cttctggctc tgggaaaaca actctactta atgtattatc tacaattgat aaaccaacaa
    12361 gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact tgtgctgctg
    12421 attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta gatagtctta
    12481 caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca gataaaatag
    12541 aaaacatggt aaagagtcta gctgaacgat ttggcatagc ttctcaatta aataaatacc
    12601 caaatgaatt atctggtggg caaagacaga gagcttcagc agttagagca atagtaaaac
    12661 atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat tcagctactg
    12721 aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt ttaatggtaa
    12781 cacatgatgc ctatgtagca agttttgcaa atagaatttt gatttttaaa gatggtcata
    12841 tcattaaaga attaaataaa caacagaata atagacatac attttatgag ttgattattc
    12901 aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac attttttata
    12961 cggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta ctttcagtct
    13021 cttttgttat tactgtattt ttcgcattta cttcattttc tgttaataag ataatgctag
    13081 aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg atatttttga
    13141 tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga cgtcgtacaa
    13201 aagaattagg tatatatgct ttacttggct atcgtaagtt tacaatatta tcgctactta
    13261 cctttgaaaa tgtattaatt tgtagtggag cttttattat tggattggta ttaggagcat
    13321 tggcacataa gggtattgtc tttggaatta cagcactact taatttatca attgataatt
    13381 cacagatacc attttttaat ataaatgcaa taaataaaac aattatcttt atagcattag
    13441 ttgttttagt cttagctgta tcaaatggta aattttttta taagactcca cttgtaaaat
    13501 tgataagata tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt ccagccttta
    13561 tggggtttat tatgatttta agcgggtatt gtttatcact agatattctt cgtggagcta
    13621 aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg attatcattg
    13681 taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa aatagtaaaa
    13741 aaaataaacg tactttctac acagatatga agataatagc tattccaaac tttatctatc
    13801 gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct actactttga
    13861 cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt tcgcgtattg
    13921 caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat gtaaaccaaa
    13981 ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca aatatttata
    14041 aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca tctaagaata
    14101 atttatccaa tagtagttct tctaaagaag ttatttcaag agaatcagga tttgaatgta
    14161 tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag ctactaaaac
    14221 aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca aataatgatg
    14281 gatatagtga aaatggaacg atttatccat tacttgttaa tggtaaagaa acacctgtag
    14341 ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt ggaacactta
    14401 tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg acaagaattt
    14461 taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat atttcagatt
    14521 atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat ttattatcaa
    14581 ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt attgctgttg
    14641 gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct gattataaaa
    14701 ttttacagaa aatgggttat actaatatac aaattaaaaa aattattaaa aaacaagtat
    14761 tgccattttt taatattccg tttttacttg gtatagtgga ctgtatattt gctaccttgg
    14821 tttataagac tgctttgatg cagaatttac ttggaaattc actttcacaa tacatacctg
    14881 tgattattgc aattttctta acttctgtag tatatttgat atattacata cttactatta
    14941 aatcctgttg taaaattgta ttaaaaaaat aatatttgcc aaatcgtcaa gcttaaaggt
    15001 gtgacgattt ggtatacttc atttataagt atttgatata atttaaaact ataaattctt
    15061 aagtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta taagattgac
    15121 aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc tcttcatggg
    15181 aatatctaca agcactgaaa cttatatgaa tattcaaagc attattccct taataatttc
    15241 aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag aaaataacat
    15301 tatttcattg ttttgtggat tactagcact agacagttgg tatttactct tatcatttgt
    15361 aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag tgtatgtctc
    15421 tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc gtaagataac
    15481 agacatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta tttctaacag
    15541 aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct ttttctatat
    15601 tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa aacctatatc
    15661 gatttcagta tttataacaa ttataggatt ccttacttat tattttttta cactaagaat
    15721 atcaaatcat attggtaatt ttggaatcta tctaactgta ctggtatttt ttatgagtat
    15781 tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt ttaacataaa
    15841 acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg tactatttgc
    15901 caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc tcattttcct
    15961 ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag ttaaaaatag
    16021 caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa cagagttttc
    16081 taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca tgatgagtaa
    16141 gtcatatcgc ccagatgttc aagatattat atatgaaaca cttaacaatc taaatacatg
    16201 tataaggaag caaatgcaag actatcatcc tgttatttta aaaaatttaa cagtaaaaga
    16261 aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa atatttgtgt
    16321 ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat tgatatatcg
    16381 actgattaaa gaattattga caaatgtgta taaacattca aatggtagcc atgcatggat
    16441 agctttagca ctagaaaaaa atataataaa attaaatatt agtgataatg gtgtggcaaa
    16501 tacatctttt aaaacgcctt tagatataac taaaattact tttgatacaa taaaacacaa
    16561 agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa gtgtttctga
    16621 taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag atggttctta
    16681 taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca attgcactag
    16741 aagaatacaa agaaatcgaa cagttttatt ccacacagga catccataat ctttcagaaa
    16801 tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga ggcttaagtg
    16861 aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact attaaaataa
    16921 taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa ataggtatct
    16981 atggattcct caataagaat attagccctg atagtttaat tgattcatta ttaaaagtct
    17041 atcaaggggt aacttgtttt ccatctgaaa atactgaagt aattattgaa gatttaacta
    17101 ctatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa ataattgctg
    17161 atgaattgta tataagtgaa agaacattat caaatcattt acaacacata tatgacaagc
    17221 tagatgtttc atcatcagtt gaggctatta caaaagctat taaaatgggt tatatttctc
    17281 cagtatatta aaaaaagtga gtgtctcaaa ataaatcttc taactcataa agcactctcc
    17341 tttgtattaa atcaaatata ttttcatcat ttaaacaatt aagaagaggc ttctctctat
    17401 tttgagacaa ctccatttta atacactatt tatatttata tataaacata ttttaaacaa
    17461 ctaaaaagca actaatatct caatacatat cttcataaca catatcacca taattacaag
    17521 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    17581 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    17641 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    17701 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    17761 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    17821 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    17881 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    17941 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    18001 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    18061 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    18121 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    18181 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    18241 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    18301 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    18361 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    18421 aatttaaata gataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    18481 atttttatta aaaagtattc tttgatattt tcattttaaa gttttaagta taataagtta
    18541 atagtgtaga tttaactttt ttatttaaac aataaactta atattatttt tataataaaa
    18601 acacaataaa aatctgatta tccgctaaaa cacagtatct atcagcattt tcaagggaac
    18661 aaatacatca atttactact tatttactac cattgaatga gccttgatac gccagttttc
    18721 ctagatatgc aaagatatgg tacagcacat cagtattgaa acaagaaaaa aattgaatat
    18781 tttatagact atggaacgcc taaaatgacg ttccttttct attttcagcc gttcttattg
    18841 gacggctatt ttattaccca aaatgaaagg agcattagat ttatgaaaaa gatgttaaaa
    18901 cgattgtgta cgggcttctt agctcttgca actgtcgtta ctgctttacc gactacaccc
    18961 gttcatgcag aaagtaagca atactggacg gagtcaaaac agcgtgtcgg tatcgttgaa
    19021 aaagtaatga atgacggttc gattggttct acctttaatg agggacattt gaccgttgag
    19081 ggcgaggacg cttactgtat cgacattaac acagacttta agaatggcta caagaccaga
    19141 gctgacgcaa gcacccgtat gagtgccgac cagataagcg acgttgcctt atctatcgaa
    19201 tatgtaaaac agtacacaga cagccacagc ggaataagca aaaatcacgc ttacctttta
    19261 agacagctgg ttgtctggca aagattgagc gtacatcttg gctggcaatg tgataatgtg
    19321 cgagcttctt atgatgaaat tccaaaggca acgcaggacg aagttttctc tggtgcaaaa
    19381 gcctttgtca aagagaataa aggacgctat gaatgtggcg gttatatcta ctctggcgag
    19441 gggcaggaat taggacaatt ctgggcgaag ctgaatgttg gaaatgcaaa actgcaaaag
    19501 acttccagta ataccagcat tacagacgat aacgggaatt actctgttgc tggtgcaaca
    19561 tacggtgtct ttgctgataa ggactgcacg aaacagcttg ccacccttac gactgatgaa
    19621 aacggaaata cagatgttgt agaggtaaaa gcaggcacag tttatatcaa ggaattatcc
    19681 gcaccagcag gatataaagt agataaaact gtatattcct taaaggttga agctggaagg
    19741 acagcgacct tgaaagtatc agatacaccg aaagtaacag acactttgat taagcttttc
    19801 aagattgata tggaaacaca gaaagacaat ccgcagggaa acgcttctct agcaggtgca
    19861 gaatttacat ggaagtatta tgctggcttc tataacaaag acaatctccc tgccgaagct
    19921 actcgtacat gggttacaaa gacaatcgcc gaaacagaca gcgacggtac aacccactac
    19981 atcacaaaat tagcggacgc ttacaaggta tctggcgaca gcttctatat gcaggacggc
    20041 aaagcggttc ttcctttagg aacactatct gttgaggaaa caaaagctcc aaacggctac
    20101 ttgctggacg gtgcatatat gcaggctggc gataagtctg aacagataaa aggcttatac
    20161 cttacacaaa ttacagagga cggcgacctt gccgtactta ctggaagtaa ccagttttcc
    20221 gtatcagaca aggttatccg tggcggtgtc aaaattcaga aacgagattt agaaacgggc
    20281 gatacaaagc cacaaggaag tgctactttg aaagatactg cctttgacat catttcctta
    20341 aatgataatg cggtattggt tgagggcaag ctatacaaga aaaatgaagt ggtaaaaaca
    20401 atccatacag acattgaggg tgtcgcttct acttccgctg accttttacc ttatggaaaa
    20461 ttccgtattg ttgagagtga agctcccgac ggttacttaa cttacggtgc aaaaccgatt
    20521 gattttacaa tcacagaaaa tggaaaaatc gtggacttaa ccgacaaagc ccattctatc
    20581 tataatcaga ttaagcgtgg agatattgag ggcgtaaaaa tcggtgcagg cacacacaag
    20641 cgtcttgctg atgttccgtt tcggatcaca agcaagacga cgggcgaaaa tcatgtagtg
    20701 gtaactgatg ataacgggca attctccact tctgctgact gggcttctca taagcacaat
    20761 accaacgcag gaaagaccag cgaggacggt gtgtggtttg gaacttctga accagacgac
    20821 agcaagggtg cgttacctta tgatacttac atcattgaag aattacgctc taaaagcaac
    20881 aagggctttg agcttatccc accttttgaa atcgtggtat caagaaataa ccttgtgatt
    20941 gatttaggaa cgctgactga tgaatacgaa aaagaaatct ctatccatac cacagcgacc
    21001 agcaaagacg gcgaaaagac tatccttgca ggaaaagagg ttacaatcgt tgacacggtc
    21061 aaattagacg gacttacaaa aggcacaaag tatcagctca aagggtggca gatgttaaag
    21121 gaagaaaatg ccgagcttat cattgacggg aaacgtgtgg aaaacgatta tacctttgtc
    21181 gctgatgatg aagaaatgaa agtggaaatt tcctatacat tcaatgcgtc tgctttaggt
    21241 ggcaaaaatc ttgttacctt tgaagaattg tatgatttca gcaatccaaa cgaacccgta
    21301 aaagttgcgg aacataaaga cattgaggac gacgggcaga cggtacttat cacagagcgt
    21361 atcatcaaaa ttcatacaac tgctaccgat aaggacggca acaaagagct tgaagcagga
    21421 aaaaaggtaa caatcattga taccgtaacc ttagagggct tagaagtcgg tacacagtac
    21481 aaacttgtgg gctggcagat gttgaaagaa gaaaatgcag agcttcttat caatggaaag
    21541 cgtgtggaaa gtgattatac gtttactgct gacagcgaaa ctatgaaagt ggaagttgcc
    21601 tttacctttg acgctacttc tcttgacggc aaacagcttg taacttttga ggaattgtac
    21661 gatttgagca atccagacga accgaagaaa gttaccgagc ataaggacat tgacgacgag
    21721 ggacagacga ttacctttaa ggaaaagcca gaagaaccag agaaacccaa aacaccgcag
    21781 acaccagaaa agcctagcag accgagcgac agtcccaaaa cgggcgacag tacaaatgta
    21841 atggctttta tcgtgatgtt gcttgtgtct gctggtgggc tggctggaac atatctttac
    21901 aaacgccgta aaatgaagaa atcataaggc ggtaacggta tgatgaaaga aaaatcacgt
    21961 tctccgccga agctgtcaaa cggcagattt ccgcttattc tggcttgtct gccaacaacc
    22021 tgcaagaaca cggatagtca agggtgcgta agcattgatt ttacccttta ctatctgggg
    22081 gattgctggt acttcccaaa ccgccagaat aagaaatcac aatcaaaaaa cttatcaaaa
    22141 atagaaagaa acgaggtaaa acaatatgga aatgaaatat gtcgtgccag atatggctca
    22201 gtcttttgga acacttgaat ttgcaggcga aagcgagcca gtctttgaaa gagataaaaa
    22261 taaccgcagg gtcttagcca gacgaagcta taacctttat tctgacgtac agaaaggcga
    22321 aaatgttgtg gtagaaattc ccgtgcaggc tggcgaaaag catttcaagt atgaacagaa
    22381 agtagaactt gtcaatccga agttatacgg cagagggtac gcaatcgggg atgtgggaca
    22441 taccgattat gtattagtag ctgatgatat tgtagcagtt caagaaaagt aaaggagtga
    22501 aaaaattatg agattatcaa acgggtttgt tattgacaaa gagaaaacat ttggagaatt
    22561 aaaatttaca gcggtgcgtg atgtgttctt acagaatgag gacgggacac cgagtaccca
    22621 gcttaaaaaa cgtatctatg atttaaaatg tagtctgcat ggtggaatta tccccgtgtc
    22681 tgtaccgcca gaagtaccgt taagggaatt tccatacaat gcagttgtgg agcttgtcaa
    22741 tcccgtagcc gatacggtat caagaaagac ctatacgggt gcagatgtgg attggtatgt
    22801 aaaagcagag gacattgtat tgaagaataa aggcaatcag aacgcaggca atccacagaa
    22861 ccatacaccg cagggacaac cgaaaaaata aacttggtgg gtagattttt attgtagcag
    22921 atatatggaa ctgttataat atgggtatga agaaagtaac acatttgaag ttgcaaaagg
    22981 agtgaaattg aaatgtgtat ggaatgttat gttgacgaaa atagaataac accactctta
    23041 aatccattgg attgtttaga aaatcataca caatacattt gtgggacttg cgggaggtgc
    23101 atttgcattg aacatgaccc caaacgtgga ttacaacggt ggaatttccc ttttaaatcg
    23161 ttagaaattg caaaattata tttgcgtact gctgattata gtaccaaaaa gtcatgtggt
    23221 atttatgaac tgaaaagtga aaatggcaga ctttcataca aaatctttgc ggacagtgag
    23281 gacttgctgt tatatctaaa aaagaacaaa ggaaaaactt gcaaagatat gaaacctgtt
    23341 tttatgattg aagaatacag agaatatgca aatacgcaga taagaaaatt gacctctgat
    23401 gaaatacaga aatatatgtc agagcgataa cttccagttt cacaatttca tatctataca
    23461 tacatagcag ttagtcttag gattgactgc ttttttcgta cccagaaagg agtgattgat
    23521 ttatgagaat gattttaaac aaaggacacc gtatcagagc cagcaacaag aaccttgtct
    23581 accacttttc catagggacg cttctgtttg tatttgtggc ggttcttctg ctactgaata
    23641 tcaaacagct catgcgtacc gattgggagc atttcagctt gttagagaac ggcttgacgc
    23701 tctcccctta caacttcata accatactga tagcgactgg tgtttgtgca ttggtcgctt
    23761 ttctgtatta ccgcttctgt tatgacaatt tcaagaaact cctgcaccga caaaagctgg
    23821 cacgaatgat actggaaaat aagtggtatg aagccgatac tgtacaagac agcggttttt
    23881 ttactgacct gcaaagcaga tcaagggaaa aaatcgtctg gtttccaaag atttattatc
    23941 aaatggaaaa gggactgctt catatccgct gtgaaatcac attaggaaaa tatcaagacc
    24001 agcttttacg gttagaggat aaattggaaa gtggcttata ttgcgagctg actgacaaaa
    24061 ccttacatga cggctatatg gaatataccc tgctttatga tatgatagcg aaccgtatta
    24121 ccattgatga agtacgggca gaaaacggct gtcttagact gatgaaaaat cttgtctggg
    24181 aatatgacgc actccctcac gctctgattg ctggtgggac tggtggcggt aaaacatatt
    24241 ttctactgac gctcattgaa gctttactgc ataccaacgc tgtcctttat atcttagacc
    24301 caaagaacag cgaccttgca gacttgggaa cagtcatgcc gaacgtctac catatcaaag
    24361 aagaaatgat agactgtgtc aatgcttttt atgagggcat ggtacagcga agcgagcaaa
    24421 tgaaacgaca cccgaactat aagacgggcg aaaactatgc ttatttggga cttccaccct
    24481 gctttcttat ctttgatgaa tatgtggcat tttttgaaat gctggggaca aaagaaagcg
    24541 taaacctact tagccagtta aagaaaatcg ttatgttggg acgacaagca ggttatttcc
    24601 ttatcgttgc ctgccagcgt ccagacgcaa agtatttctc ggacggtatc agagataact
    24661 tcaatttccg tgtgggattg gggcgtatca gcgaattagg ttacggtatg ctttttggtt
    24721 cagatgtgaa aaagcagttt ttccagaaac gtatcaaggg gcgtggctat tgtgatgtgg
    24781 gaacaagcgt gataagcgaa ttttacacgc ctttagtccc gaaaggacat gattttttgc
    24841 agactatcgg ctctcttgca caagcaaggc aggacgggac ggcgacgtgc gaagcgaaag
    24901 gcgacggcac ggactagccg ttgctggtgt ggcgaagcca cgccagcagg taaaacccct
    24961 cggtatctaa cagaggggta cgtttgcaaa tagacaatag acgaaaaaca taggaaacat
    25021 aaggcttctg gcatggtttt ctagcaaaat tcagttgtga agtcgcctaa aaaaacttca
    25081 cactttacag attgggggta tggttctgaa tgaagaacaa tggataaaag aattacggga
    25141 gaaacggatt gcttatggta tctcacaagg caggctggcg gtggcttctg gtatcacaag
    25201 ggaatatctc aacaagatag aaagcggaaa aatgaagccg tcaaaggaac ttcttaatac
    25261 tttgcataag gaactggcaa ggtttaatcc agaagcaccg cttaccatgc tgtttgatta
    25321 tgtaaaaatt cgttttccca cgctggatat acagcacatc atcaaagata tattaaaact
    25381 gaatatcaat tatatgctcc atgaagatta cgggcgttac agctatacgg agcattattc
    25441 tttaggggat atctttatct atacgtcggc tgacgaagaa aaaggtgtcc ttttagagtt
    25501 aaaggggcgt ggttgccgac agtttgaaag ttacctgctg gcacagcaaa gaagctggta
    25561 tgacttcctc atggacgcac tcatagacgg tggcgtgatg aagcgtatcg acctagctat
    25621 caacgaccat acgggcattt tggatattcc agagcttgcg gaaaaatgca ggaaacggga
    25681 atatatcgga aagtccagaa gctataagtt ttaccagtcg ggcgagctta tcaagcacag
    25741 agaggacgac agagaatata tgggacgtac cctttatctt ggttctctga aatcagatgt
    25801 gtatttctgt atttatgaaa aggactatga gcagtacgtc aagttaggga cacctctgga
    25861 agaagccgac attatcaatc gttttgagat acgacttaga aatgaacgtg cctattatgc
    25921 agtacgagat ttgctgacct attatgacgc agaacagacc gccttttcta tcatcaacca
    25981 gtatgttcgg tttgttgatg aagaaccaga caagcgaaaa aatgactgga aactcaatga
    26041 ccgctgggct tggtttatcg gcgataacag acagagcttg aagctaacga caaagccaga
    26101 gccttacacc ttagaccgta cattgcggtg ggtacaacgg caggtagcac cgaccttgaa
    26161 aatgctgaaa aagattgata agggaaacgg tacagactac atggaaacaa tcgaacagca
    26221 ggcaaagctc acagaaaaac atgaaatgat aatcaaacag cagacgaccc ctgcaaaaga
    26281 tttagtagaa agttaggagt gatgaaaaat gtggatatta ttaaaagact tcctgctggt
    26341 atctatggga atgggtatcg gtgtggtctt gatgtgtatt ttgaatgtcg gcaaagaagc
    26401 tgactatgaa atgaaacaat tagaagaaag cgaggacaat taaatgaatt tcggacaaaa
    26461 tttgtatcaa tggtttttat caaacgcaca gagcttagtg cttatggcga ttgtggttat
    26521 cggtatctac ttagggttca agcgtgagtt ttccaaactt atcggtttct tggtagttgc
    26581 cttgattgct gtcggcttgg tattcaatgc tggcggtgtg aaagatgtac tgttagagct
    26641 gttcaataag attatcggtg cataaaattt tattgtggtg ctgatatgag aatggtataa
    26701 tgtggatata taaaattctg atttatgggg gcgttatttt gaagaaagca atgatatatg
    26761 ttcatggtaa aggtggaagt tatcttgaaa ctgaacagtt caaaaatatt tgtttagatt
    26821 ttgatgtttt tggagtagat tataatgact atttcccgtg gattgtaaaa aataaaatta
    26881 tggaagtcta cgataaaata tctgaaaaat atgactacat atatgtactt gctaatagta
    26941 tcggagctta ttttacaatg cacacattac agaatttcaa gattgaaaaa gcattattca
    27001 tatcgcctat acttgatatg gaacaactta tagtagatat gatgagctgg gcgaatgtgt
    27061 ctgaaaagga actttctgaa agaaaagaaa taccaactga ttttggagaa acattatcgt
    27121 gggaatatct atgttttgtt cgggcaaacc cgataaattg gaaaacgcct actgaaattt
    27181 tgtatgctga acatgataat cttacatcac gtcaaacggt agataaattt attgatagac
    27241 attgtgcaaa tcttaccgtt atgaatgacg gagaacattg gtttcatact gacgaacaga
    27301 ttttatttag agataattgg ctaagaaaag ttatacaata gaatttagat tttatatcca
    27361 cacacaaagc agttagtttt aggattgact gcttttttct tatcaaaatt tcagattgga
    27421 gtgatgaaat ggtgtctgtt agagctgttc aataagatta tcggtgcata gaattttatt
    27481 gtggtgctga tatgagaatg ttataatgtg gatatgaaac acagtatcta ggagtgatga
    27541 aatgaaatat ataatgcttc atggtttggg acaatcttct gaaagttgga gtgataccgt
    27601 aaaagtattc aatgatgatt ttgaagtttt atgccctaat ttatcagatt ggctttctgg
    27661 gaaaacacca tgttatgata cattgtatcg gacattggaa acatactgtg aacagtttga
    27721 agaaccgcta aatatttgtg gactttcttt aggtggtatt cttgctatgc agtatgcgat
    27781 agaacaccca gaaaaaatga actcattagt gttaataggc acacaatatg aaatgccaaa
    27841 gtatctgtta aaaatgcaaa atctgatatt ccgtattatg ccttgttcag catttgaaaa
    27901 aatgggcttt cagaaaacag attttatcaa tctatgcaaa tctatgacag aacttaattt
    27961 taaacatgaa ctgaaaaaaa tatcatgttc ggtacttgtt gtctgtggcg ataaggataa
    28021 gataaataaa aaagcttctt tagaattgca acaacagata gaaaacgcag aaatagctat
    28081 tattgaaaat gcaggacatg aagtaaatac agataaccca acactactag ggcaaaagtt
    28141 aaatgtattt ttcaaacaat atgtctgata aacaatttca tatccataca cacaaagcag
    28201 ttagtcttag gattgactgc ttttttctta ccaaaatttc agattggagt gatgaaatga
    28261 gcgatatttt taaggatatg caggcaaacg tcggctgtga atacatttcc gacctgccct
    28321 cttacaagcg taaggtatgg caggaaatga aacgactgaa ccctgccaat tatgaagaaa
    28381 gacagttaga agatttttca aagtatgtgt ttggtatgtc gtaccagacc ttaaaagatg
    28441 tgatgaaaca acagaaagga cgtgaggaac aatgcaggaa acaagggtgc tggtggaaac
    28501 gagaggaaca actggcgaag aaacaatatc atactggttt gacctgccga tagatgttgc
    28561 cgaatttgaa gaaaagttag gtatcggtgc agaaagtgga gattaccgta ttatcgaaaa
    28621 ggtgttgcct tatgctgatg aagtccacga acatacaagc gtgtaccagc tcaacgaatt
    28681 agattttatg taccgccagc tttcaagtga tatgcaggaa gaatatgtat cactacttac
    28741 ggtgtttgaa aatttagaag cactttatat ttgcaggaat gtgattacgg tttatcccaa
    28801 ctgcaaaagc atgatagatg ttgcaaagca aaagctgatg aacgacccga cgttcaaaca
    28861 tttatccgag gactgtcaag cctactattt tgactttgaa gcctatgctt ctcacttgca
    28921 ggaacacggg aaatttttag taacagaaca cggtatcttt gaactgccag agtaggaaat
    28981 gaggtggtgc ttatgattga tgatatggcg gtttacattg ctaatcttgg caaatacaat
    29041 gagggctatt tagtcggtgc ttggttcaca tttcctattg gcgaggaaga tgtaaaagaa
    29101 aaaatcggct tgaatgaaca gtatgaggaa tacgctatcc atgataccga caacttccct
    29161 attgcgattg gcgagtatgt ttccattgaa gaactgaact ctatgtatga aatgatagag
    29221 gaacttcccg actatatcgt agaatgtctg gacgaattta tcagccacta cgggacgctg
    29281 gaagaagtcg tggaacacaa ggacgatatt tattattatc ccgactgtga aaccatgaca
    29341 gacgttgcct attactacat agacgaattg caggcacttg gggatattcc acccagctta
    29401 cagaattaca ttgactatga agcctacggg cgagatttgg atatgggcgg ttgcttcatt
    29461 gaaacaagcc gaggtatgtg cgagatacca tattaacgct ggaagatcag caacaagcat
    29521 tgttgctact gacagcgtat ttctctttta agggacagtc tgaaacatgg ctgtcctttt
    29581 ctatttagaa acttacgaaa ggagctgaaa gaacttgaaa aagattaaaa gctacacggg
    29641 tatctggaac gtggaaaaag tcttatatgc aatcaatgat tttaacctgc cttttcccgt
    29701 tacttttaca cagattacat ggtttgtgat tacggaattt atcatcattc tgtttgggga
    29761 tattccccca ctttccatga ttgagggagc atttctcaaa tacttcggta ttcccgttgc
    29821 tctcacttgg tttatgtcgc agaaaacctt tgacggaaag aagccgtaca gctttttgaa
    29881 atcacagata acctatgccc tgcgaccaaa aatcacttat gcaggaaaag ccgtaaaact
    29941 gcataagcag atactcaatg aaacaatcac ggcagtaagg agtgtgaact atgttcccga
    30001 taaaatatat tgacaataac cttgtctgga acaaggacaa tgaggtgttc gcctactatg
    30061 agctgatacc gtacaactat tctttcttat ccgcagagca gaaatttatc gtgcatgaca
    30121 gctttcgaca gctcattgca cagtcccgtg agggtaaaat tcatgcgttg cagattgcca
    30181 cagaaagctc aatacgaagt atgcaggagc agtcaaagaa actggtaacg ggaaacttaa
    30241 aggaagttgc ctaccagaag atagacgaac agaccgaagc gttagtatct atgattgggg
    30301 ataatcaagt ggactaccgc ttttttcttg gctttaagct catggttacg gaagaacagc
    30361 tcaatctgaa gaacatcaaa aaatcggcgt ggctgacgtt cacggaattt ctccatgaag
    30421 tgaaccacac gctgatgaat gattttgttt ccatgccgaa tgatgaaatc aaccgttaca
    30481 tgaaaatgga aaagttactg gaaaataaaa tctcccgtcg ctttaaggtg cgtcgcttgg
    30541 aaatcaatga ctttggctat ctcatggaac atctttacgg cagggacggt atcgcttatg
    30601 aagattatga gtaccagcta ccaaagaaga aattgcagaa agaaacgctg ataaaatact
    30661 acgaccttat ccgtccgaca aggtgtgtga ttgaggaaag ccagcggtat ttaagattgg
    30721 aacatgagga caaggaaagt tatgtgtcct attttaccgt caatgcgatt gtaggcgagc
    30781 ttgattttcc gtcaagtgaa atcttctatt tccagcaaca gcaattcaca ttccccgttg
    30841 atacttctat gaatgtagaa atcgtggaaa atcggaaagc attaacgaca gtccgcaaca
    30901 agaaaaagga attgaaagat ttggataatc acgcctatca agctggaagt gaaaccagct
    30961 caaatgtggt ggacgcttta gacagcgtgg acgagctgga aacggactta gaccagagca
    31021 aagaaagtat gtataagtta agctacgtca tacgggtgtc tgcacccgat cttgacgagc
    31081 tgaaacgccg ttgtgatgaa gtcaaggact tttacgacga cctcaatgta aagctggtgc
    31141 gtcctgctgg ggatatgctg gggcttcatt ctgaattttt accagccagc aagcgatata
    31201 tcaatgatta tgtgcagtat gtaaaatcag attttctagc agggcttggt tttggtgcga
    31261 cacagcagtt aggagaaact acgggtatct atatgggcta ttctgttgat acaggaagaa
    31321 atgtgtacct gcaaccgtcc cttgccagtc aaggtgtaaa aggtacagtt accaacgctt
    31381 tagcgtctgc ttttgtcggt tcgcttggcg gtgggaaatc gttctgtaac aatcttctgg
    31441 tatattacgc ggtgctgttt ggcggtcaag cacttctgtt agaccctaaa agtgagcgtg
    31501 gcaactggaa agaaacactt ccagaaatcg cccatgaaat caatatcgta aacctaacaa
    31561 gcgataaagg caatgcagga cttcttgacc cgtttgtgat tatgaagaat gtaaaagacg
    31621 ctgaaagtct ggcaatcgac atcttaacat tccttacggg tatttcctct agggacggcg
    31681 aaaaattccc cgtacttcgt aaggcggtac gctccgttac ccagagcgac agtcggggct
    31741 tgctccatgt gatagacgag ctacgccgtg aagatacacc catatcaaga aatatcgcag
    31801 accatatcga cagcttcacg gactacgact ttgcacacct gctgttttca gacggtacgg
    31861 tagaaaatgc aatcagtctg gataaccaac tcaatatcat tcaagtagcg gacttagtat
    31921 tgccagataa ggacacgacc tttgaggaat acacgaccat tgaattattg tcggtgtcta
    31981 tgctgattgt gattagtacc tttgcccttg attttatcca ttcggacaga agcattttta
    32041 agattgtcga tttggacgaa gcgtgggcgt tcttaaacgt ggcacaaggc gaaactttat
    32101 caaataagct ggttcgtgct ggacgtgcta tgcaggcagg cgtttatttt gttacacaat
    32161 cttctgggga cgtgtcaaaa gaaagtctga aaaacaatat cggcttgaag tttgcctttc
    32221 gtagtaccga cttgggcgag ataaagcaga ccttagaatt tttcggtatc gacaaggacg
    32281 acgagaacaa ccagaaacgg cttcgtgatt tggagaacgg acaatgttta ttgcaggact
    32341 tatacgggcg tgtcggtgtg gtgcagatac acccagtctt tgaagaactg ctacacgcct
    32401 ttgataccag accgcccgtg cagagaaatg aggtggagtg atgaaagaaa ggataaaagg
    32461 tgcgttcaca aaaaagaaga ttttccactt tctcaaaatg tctctgtttg tcgtggcact
    32521 ctcccttatc ctgctttcac ttctggggac ggtggctcat gcgacggggc ttgtggacga
    32581 taccataaac gcagaaaatc tatactcaaa atatcccctt tccaactacc agcttgattt
    32641 ttatgtggat aatagctggt cgtggctacc gtggaactgg ctggacggta ttggaaaatc
    32701 ggtgcagtac gggctttact gcattaccaa ttttgtctgg acgataagcc tttatttaag
    32761 caatgcaacg ggctatgtgg tacaggaagc ctataagctg gacttcatca atgatatggc
    32821 agacagtatc ggcaaaagca tacagaccct tgcaggcgta acgcagaacg gcttttcttc
    32881 tacgggcttt tatgtcggtt tcctgcttct cattatctta gtggtgggaa tgtatgttgc
    32941 ttatacggga ctgataaaaa gagaaaccag caaggcactt cacgctgtta tcaactttgt
    33001 ggtggtgttc attctgtccg cttcttttat tgcctacgct cccgattaca tcaagaagat
    33061 aaatgaattt tcatcagaca tcagtaccgc ttcacttgat ttgggaacaa aaatcatgct
    33121 ccccaactct gacagtgagg gtaaagacag cgtggacttg atacgggaca gcttattttc
    33181 tattcaagta gaacagccgt ggctactctt acaattcggg aacagcaacg cagaagaaat
    33241 cggtacagac cgtgtggaag ctctggtatc ggcaagtcca gaggacgagg acgggaaaac
    33301 cagagaggaa gtcgtgaaaa cagagattga ggacaacgac aacaacaatc tgacaatacc
    33361 gcaggtagtg aaccgtttgg gtatggtgtt tttcctcttg ttcttcaact taggtatcac
    33421 gatatttgta ttcttgctta cgggtatgat gttgttcagc caaatacttt ttattatctt
    33481 tgcaatgttt ttgcctatca gctttctact ttccatgata ccgagctatg aaagcatggc
    33541 aaagcaggca atcgtgaggg tatttaatac cataatgaca cgggcaggaa taacgctcat
    33601 tgtaacggtg gcatttagta tttccagtat gttttacaac atttccacag actacccatt
    33661 ctttatggtg gcgttcttgc agatagtatg tttcgctggt atctacatga agctgggcga
    33721 tttaatgagt atgttctctt tgaacgctaa cgacagtcaa agcatgggac gcaggatatt
    33781 ccgcagaccg tatctgttta tgcgacatag ggctaggtgt atggaaagac ggcttgctgg
    33841 tgcggttact gctggcggtg ttgctggtgc ggtggcaggt agaacgattt caaacagaaa
    33901 accgacaagg aacacagcta caaaagacag tcggggcaat accacttcca gcatgggaca
    33961 gcgtgcaggt tcaaaggtgg gggctgtctt agatacgaaa aataaagtga aagacaaggc
    34021 aaatgctgtc aaagagaata tcaaggatat gccgacacag accgtttatg cggtgtattc
    34081 cgcaaaggaa aaggcaaagt ccagcgtgtc cgacttcaag cgtggcatgg tgcaggaaca
    34141 gcagtccaga cagacgggac gcttggaaaa gcaggaacag catagacaaa atatcgctga
    34201 caagcgtatg gagcttcaaa aggcacaaga agcaaggcag gcacagcgaa aggctgacgg
    34261 atcagcgaca acgggagcta cccgtcccca tgagcgacca gccacagctt caaagccgag
    34321 tgcggaaaaa atgcaggaag tcaaacgtcc tgccacagcg accacttcaa aagcaagtga
    34381 gccagtcaag acaaatgtta tcaaagagcg tccgttattt tctggtgctt ctgataagaa
    34441 agcgacccag tcggcacagc cagtacatag gcagaatgta gaaaaagtgg tatcgcagga
    34501 aacacgccag aattacacaa aagaccgcag gacaaaggtt cagcagacgc aaaccgtcca
    34561 aaagaaccag cagacaacag agaaaactcc taaccttgtg atgaagaaag gacagaagaa
    34621 aaaatgaaac tgaaacatat cgctatcatt ggcagtctgt ttcctatcct cttttctctg
    34681 gtgcttttct ttggtgtgtt gattagtgcg gacagcgacg acgagaacag caatttttct
    34741 tctggcatta cgggtatgaa cttatccgca gaagtcttga aacatcagcc tatggtagaa
    34801 aaatacgcca gagaaaacgg tatttccgag tatgtcaatg tgctgttggc tatcattcaa
    34861 gtagaaagtg gcggtacggc agaagatgtt atgcagagtt cggaaagtct tggtttaccg
    34921 cccaactctt tagatacgga aagttcaatc aagcaggggt gtaaatattt tgcgtccctg
    34981 ctttcttctt gcaaaaatca aggtatcgac gatttgaatg tagcgataca gtcttataac
    35041 tatggcggtg gttatgtggg atatgtggca ggaaaaggaa agaaacacgc ttttaatctt
    35101 gcggaaaatt ttgcccgtga gaaatcgggt ggaaagaaag taatctacac caacccgata
    35161 gctgttgcga agaacggggg ctggcggtat ggctatggaa atatgttcta tgttgaatta
    35221 gtcaatcagt atttaactgt ttcgcaggta tcgggagagc tggcacaaaa ggtaatgaat
    35281 gaagcgttga aatatcaagg ctggaaatat gtgtatggcg gtagcaatcc caacacttct
    35341 tttgactgtt cgggactgac gcaatggtgt tatggaaaag ctggtatctc cttaccgaga
    35401 acggcacaag cacagtatga cgctacccaa catcttccac tttcgcaggc aaaggctggg
    35461 gacttggtgt tttttcattc tacctataat gctggaagtt atgtaacaca cgttggtatt
    35521 ctcgtttcgc cgacacagat gtaccatgca ggcaacccga taggatatgc agacctaagt
    35581 agtagttact ggcaacagca cttaatcggt gcaggacgag taaaacaata gaaaggactt
    35641 gaaaatatgt ttaagaagaa taagaaacag acagaaacta tcaaagaacc aaaagaaaaa
    35701 aaggtgcgta ctgttaaggt aggcacacat aagaaagccg tgattgcgtt gtgggtggta
    35761 cttattgcaa gcgtgagctt tggggtgtat aagaatttta ctgctatcga ccagcacacg
    35821 acccacgaaa aagaaatcat tgaacttcgc ttgcaggaca ccaacgggat agaaaatttc
    35881 gtgaagaatt ttgcgaagtc ctattacaca tggaataaca gtaaagaagc gattgaatca
    35941 aggacgcagg caatcaacgg ttatctgaca aaggaattgc aggacttgaa tgttgatacc
    36001 attagaacgg atataccgac cagctccaca gttacagatg tgattgtatg gagtatcgaa
    36061 caatcgggaa cggacacttt ttctgctacc tacgaagtag atcagcagat aaaagaggga
    36121 gaaaagacaa gcaatgtgaa agcaacctat accgtaaaag tccatataga cgctgacggg
    36181 gatatggtaa tcgttcagaa ccctaccctt gcaccagcaa tcgaaaaatc agactatgag
    36241 cctaagacac cagaagcaga cgcaagtgta gacgctgata ctgtaaacga cgctaccgca
    36301 tttctggaaa cattctttaa gctgtaccca acagccacag aaaaagaact tgcctattat
    36361 gtatctggta atgtacttga acctatcggc agggactatc tgtattctga attggtaaac
    36421 cctatcttta caaaggacgg ggacaatgtg aaagtcaagg tagcggtaaa attccttgat
    36481 aatcaggcaa aagcaacaca agtatctcag tatgagcttg tgttacaaaa ggatagtaac
    36541 tggaagattg taggataaag aaatatggcg tgcatttcta taagagattt gcacgctatc
    36601 catgttgtaa ggaattgaca attcttattt tttgcattat actattcatg taaatatgaa
    36661 aagtatgatt tacaaataaa tatgataata aggagataaa tgatgaaaaa aggacaacat
    36721 atttttggaa cggtaaaagt tggagaacgg ggacaaatag ttatcccaaa agaagcccgt
    36781 gaaatttttg atattaacgc tggcgataca cttttagttc ttggcgacga agaacaagga
    36841 atagcgattg tcaaggcaga tgtaatgaaa gaactggcag taaaaatttt aaaagggctt
    36901 ggtaatttgt gtcttgacaa ctgaaccact atattgagtg gtgggtttaa tgatgaaaaa
    36961 tagcatttct ctattcttgc cattagcctt attgctggat ttaactatcc cttttttcct
    37021 cgctccgttt tataaaggtt atagtcattc tatgcaggta atgagcgtgc ttggaaataa
    37081 caaatctcca ctacatttta tatacaactt atggctagtt ttatgcggta tcattctttt
    37141 aatgagtggt tttaagattt atgaaattgt ttcgcagaaa tcaaaaaaca tcgctgttat
    37201 tttgtttata gtcatattga tatatgcgat aggtggctgt attctctcag ggattttttc
    37261 ggtaggagaa acaaaatctc tcaatactat tcctgaaaaa atacatggtt acggggcggt
    37321 tattggattt atgtgtttga cttttgctcc gcttttgatt agcttatatt ggtttcgaat
    37381 taaggaaata ccacttgcac tattttcaat gatttgtttt cttctagcat ttatcttttt
    37441 tacgattttt gtgatggcag ataaaccaaa ctacaaagat acaatcatag cttttgaggg
    37501 cttatggcaa cgattatcac tactgtttat gtatttacct ttgggttgtt taatattatt
    37561 ggaaaaataa attaggagtg aactctatga taggactaaa aaaacgagat ataaaaaaag
    37621 ccttgaaagc tggtgcaaaa gctggcggtg tatcattggc tgatgttcgg gctgatattg
    37681 aagcgacgat tgatgaagct atgaatagta cagacccaga ggtgcaagca aattttaaaa
    37741 agtattttgg gaataaacgc cctgcaccag aagaatatat ttatataatt acaaagaaaa
    37801 cagttaaagg gctgaaagta tgaaaataga acgtctgata ggaataatct tttatttaat
    37861 aaatagagag tgtgtaacaa caaatgaatt agcacaaaag tttgaagtat ctcgcagaac
    37921 gattttacga gatattgata cactaactct tgctggtata ccgatttatt ctgagttagg
    37981 tgtaaatggt ggctatataa taaataaaga ttttaaggtt gatgaaaaaa ttattgacag
    38041 caacaataaa gaatatattt tgcttgcttt aaatagtcta agaactgttt acggaaataa
    38101 aaagatcatt gaaacttacg aaaaaatgaa acatctttat aacgatgtca atattaatgc
    38161 acttccagat gttgatttct ctgttgtaac tgaaagtcca caaattataa ggtgcgtaga
    38221 cttaatcaat aaagcaacaa aagaacatcg aactataaaa tttatatata ctaattcagc
    38281 aggacataca aaaaaagttt ttctaaaccc attacataca tattataggt ggtactcctg
    38341 gtatgttttt ggatatgaca cctctaaaaa tgattatcgc atgttcaaag tggtaagaat
    38401 gaaaaatatt tctttaacaa aagaactttt tcaaaccaaa gataatatag ctgacctttt
    38461 atctgatttt gaaaaaagac gcaaccagac aaatattacg gttactctat cttataaaca
    38521 agaaatagac accttagtta atgaatactt taaaggaaaa attgttgaaa ctgtcaatga
    38581 ctgttttata agagaaattg agattaaaga aaatgatttt atgacattct caatcctttt
    38641 aggactttgc gataaagtaa aaatattatc ccctcaaaaa tacaaagaaa aggtattaac
    38701 tcatatcaga aaaataaaga aaaatttcac ttaatagtga catacatttg tcactattcc
    38761 tgccatataa ttttcttata tcgaaaatat gaggagatgt gaaaatgaaa aaagtatatg
    38821 tatatttatt agacgacatg gcagaatgcg aaattggtta tattttacaa gcattcagta
    38881 tggaaaaatt attaaaaaat ggaactaaag aatttgaagt aaagacagtt ggttttaata
    38941 aagaacccgt cttaacttta ggaggattaa caattgttcc agattatagt atatcagaag
    39001 ttgacttcaa tgctagtaca gctctgcttt taccgggttc ttccacttgg ggaagtaaag
    39061 aaaatcaaga aatattggaa aaagcacaag aatgtttaaa caaaaatatt ttagtaggtg
    39121 ccatttgtgg ggcaacactc gcacttgctg attatggtat attagataaa tttaaacaca
    39181 caagtaattc tctagaatat ctaaatttct tttcaagaaa ctatgcagga cagagcttgt
    39241 acgtttgttc taattccgtt ttagacagaa atttagtaac agcaaattca gcaggttcat
    39301 tagaatggac gaaaaatatt ctaaattacc ttaatgtgta ttctgacaat aaaattactg
    39361 ctttttacaa ttattactcg acaggaaacg caaaatacta tgatgaactt ttaaaataaa
    39421 ggtggtgttt ctgtaatgaa acaatcatgg gtattatgcc catctgcaat aacaagacac
    39481 gaacaaaaat acgggtagat acggaactga taagtttccc tttgttctgc ccaaagtgca
    39541 agcatgaaac tttaatcaat ataaagcatg gaaatgtaac gactattaaa gagccagacg
    39601 ctaagacgca gagccgatag tttataggtt attgtaactt atagattgtt ggctcttatt
    39661 tgttacctgc ggagcggatc agcaaataag ttttggaata taatggaaaa acatttatat
    39721 aaaagaagaa acatgaaaat attagtaagt gcctgccttt tacaatataa atatactaaa
    39781 aaaatcgtaa aaagatcctc aataattcat atatttttac taatgaaaga tcaatatgac
    39841 ataccaaagc gttatttgct tctattcaaa agagtgtcaa tttcttcttc tgataatttt
    39901 tgcttgaaat taaacacatt atctacagct gttcctaaaa gtcttgccaa agggcttagt
    39961 aatgaaatat caggatatgt attgtttgtt tcccattttg acaccgaaga agtagttatt
    40021 ccaagagcat ttgcaacttg ttcttgtgtt agacctttta tttttcttag tttctgaata
    40081 atatcaccga taaataattt cattttgtga catctccttt acttaatgaa aatattatca
    40141 gataaaaact ttttttaaca atgcaggtat cgcagtatga acttgtgtta cataaggata
    40201 gtaactggaa gattgtagga taaatcaaaa aatgccgtag ctttattttg catttattgc
    40261 aagttacacg ctacggctag attaaaatat aatattttga aatcttcata aaatctttaa
    40321 ttttgctttt tatactttaa ctaaaaaata aggaaagggt atgaacaatt atgaaagcaa
    40381 ttttaaaaac aaataaccta tgtaaagatt tcaaaaagca aaaggcagtg aaaaatgtat
    40441 ctataactgt ccgtgaaaat tcaatatatg ggctactcgg tccaaatggt gctggaaaat
    40501 ctaccacttt gaaaatgatt acgggtatgc tccgtcctac tagcggtaaa gttcttttta
    40561 atggacatga atggaacaga aaagatttag aacagatagg agctttgatt gaaactcctc
    40621 cactttatga aaatctttcc gcagtagaaa atttagaagt ccgtgcaaaa ttactaaata
    40681 ttcctaaaac ccgaatagat aaagttttaa aaatggtaga tttgcaaaat acaggaagga
    40741 aaaaagctgg acaattttca atgggaatga aacaacgttt gggaatagct atcgctttgt
    40801 tgaatagtcc caaattgttg attttagatg agccgacaaa tggattagac cctatcggca
    40861 tacaagaatt acgttctctt attcgttctt ttccctctaa agggattact gttattctat
    40921 ccagtcatat tctatcagag gttcagctta tcgctgatga tattggtatc atttcaaatg
    40981 gtattttagg atatgaagga cagatgaata aagatgaaaa tttagaaaat ctgtttgtag
    41041 aagttgtaag gaaatcacag gaggaacgat aaaatgaaaa attatatatc ttctgaatat
    41101 ttaaaacata aaaatacatt cgcaaaaaaa ttagtatggt tagctcctat actaacttta
    41161 tgtctgaata tacttgctcc tatgtggtat caacaaaatt cgtataactg gtggtatgta
    41221 ttcttatatc ctggttgttt aacgctatta tctattttga taattcaaag agataacggc
    41281 aaattaaaat ttagagcaat tttgtcattg cctgttgact taaaaaaagt atggtatgca
    41341 aaaataatca cttgtataat atacattgta cttgcaaata caattttgat gttatgcaat
    41401 gttgcaggtg gttttatcat tcaacacatt tttgatatac ctatgacaat tagcccaatg
    41461 caggcattgt tcggaacact ttgcattatt ctggcaagta tatggaatat tccattatgc
    41521 ttgtggttta caaaaaaagt gggtgtattt gccactctca tattaaatgt cggaatgagc
    41581 tttatgttgg gaacattagg ggcaaataca agtttttggt tcatatgtcc ttatagctgg
    41641 gttactcgcc ttatggttcc cacattagga atattaccga atggagaatc agtaacagga
    41701 agtgctttat caactccttt accgttggtt attatgacaa tctgcttgtc attgctttta
    41761 ctatatacaa tttcaaagtc aacggcaaaa tcttttataa agcaggaggt tctttaatgt
    41821 ttagtattat cagatgtttt caagcagaat ggcttaaaag cagacataca ccattacttt
    41881 tagtccatgt gctttttccg ttaataggag ctggtgtttt tgcaggatat tttcatatat
    41941 ccggttggag tgagatgaca aatgtaacga cctttttaga ggtgttagca attatttttc
    42001 catttgttat tggtatcgtt gtaggtatga tagtcgaact agaaaatgga gcaggtcatt
    42061 ttcagttaat cttaggtacg atccgttcac gtatagcggt atatatcgga aaattagtat
    42121 atcttatcgt tttagcaacc tttgcaacaa tactttgtat tttcttgttt gccgtactct
    42181 atccagttat gccattttcg ttttatataa aacctcttat tatgttgata ttgtctgtaa
    42241 taccacttta tcttatttcc atacctgttg gtttcagttt aggcaaaagt gtatctatgg
    42301 gcataggtat tgtaggaagt ttattatctg cattgctagt tacaggactt ggagatttta
    42361 tctggaaatt cctgccgtgg ggctggggtg tacgatttat ggattattgt gttttaagat
    42421 atgttaatga ggggcagttt cattctaact tacccgaatt tcaactaggt tttatactca
    42481 tgctggtttt tacagtagtg ctgtttatca taagcttagt gtggtttcag agatgggaag
    42541 gagtaaaaga aaatgagtaa aaaaatgatg atattgctgt ttgctgttat tatatgttta
    42601 tctcttgcag gctgttcttt acaggacaaa atcggggact attccagaga gcaatgctat
    42661 ctgaataagg aaaatgtgac gcaattttcc tataagggaa atgactacac gattttagcg
    42721 gatactgtct ctaatagtgg actcggggaa tggattgggt atatccgaca acttgcggct
    42781 gtagacgaat caggaaaaat attacttcaa gaaaatctca aaactgctac ctttcagacg
    42841 ttggcagatt tagcagattt agtggataaa gcccctaatg acgcctatat tattccattc
    42901 ttaaatgtgt atgcagctcc taacgctgac gactatctga ttgtagatat aaatggaggg
    42961 tatcacaaag ctgttataga tgaaaatatc aaaggcacag atacagtttt tgactttaag
    43021 gatatagagc aatctatgag tggaaagttt gaaatcaatc ctcaaaatgc aacgcagctt
    43081 ctttgtgatg ggacggttta tcaagtaact tctgatactg tatcaaataa tgaattggga
    43141 agttacattg gtattcttgc ggagaatgtc atatttaatg cagaaacaaa aattcctttg
    43201 tcaaaggagg aactaagaaa aattgactgg tatggggaaa acgccggaca acatcgggaa
    43261 caatggattt acaaagatat ttatgaaatt catggtacag aaaagaccga agccgttgca
    43321 gtacagataa atgaccgcta ctatattgca aagcgtcaat gaccgatttt tgtttttctg
    43381 cctttatgga ttatgatata ctaaaaaagc taggaggcga tactatggca acaatcctta
    43441 tgattgatga tgaacgacct attttagaac ttgtgaaaaa tggactgcaa aaggacggac
    43501 atttagttac agtctatacg tccgccgcac aggttcctct tgataaactg aacagatatg
    43561 acttgattat actggacatt atgatgccgg atatagacgg gttttcttat tgtgataaaa
    43621 tccgttcatt agtggactgt cctatcctct ttctgactgc taagacaatg gaaaatgata
    43681 ttacattcgg tcttggatta ggagcagatg actatttgac aaaaccattc cgtattacgg
    43741 aattacgggc aagagtaaat gcccacttac gccgcgaaca ccgagaacga catatagctc
    43801 ttacatttga ccggataaaa attgatttat ctgcaaaaga attacgggta gataatatac
    43861 cagtgccttt aacgaaaagt gaatatctga tttgtgagta tcttgctagt aacaaaggac
    43921 aggttttttc aaaggaacaa atatacgaag ctacattttc cctttatgga gaaagcgata
    43981 gttctacaat ctcgacacat ataaaaaata taaggtcaaa gtttgagaaa acgggtgttc
    44041 agccaatcgt aacagtttgg gggataggtt atagatggga ataaaaatga aaacatcact
    44101 tacaagaact ttttataaat ttcttgtttg tctacttggc ggactatgct tgtccgttgt
    44161 cgtccctttt gcctgtatga tgtttgcttc cagtataggg cttgtaacac ttgcagataa
    44221 taacgaacgg caaacgaaag ctttagtacc gatactaact gcaacaccaa atgtatcaga
    44281 tatacatttt cctactggaa ctaaatttct tcgattaact aaaaactatg gatacataga
    44341 tacgaatatg agtgaagaag aaaaagaaaa ggctttaatg tttgctaaaa cggggttcat
    44401 ggatacaagt ggtaaaacac agtttatttt cattactcga gatgatgaat atattgtttt
    44461 acagtatgtt ataggttctc aatatttaaa tgcacaattt aatcagtatt taccgtcccc
    44521 agaaattatg atgatttctt taatcactat aagcagctta ggggtatgtg tttatctgac
    44581 tgttcatttt gctaaagagc tgagaaaaga attgacacct attttagaag ctaccaaaga
    44641 aattgagaat aacaacttag attttactat cggacattct aaaatattgg aatttgaaaa
    44701 cgtgattata tcctttgata gtatgcgaaa tagtttaaaa gggtcattag aaaaacagtg
    44761 gcgaagtgaa aaagcacaaa gagaacagat tgcatcttta gctcatgatt tgaaaactcc
    44821 attaactgtt atgcagggga acattgacct attagatgaa acagaacttg acgaagaaca
    44881 aaagctatat ttatcgtatg caatgagtag ttctgagcag atgaaacagt atataaaaat
    44941 tttgattgat atatctaaag catctgctgg ctatcaatta caaatggaag atgtaaattt
    45001 tccagagttt tggaaacatg tcttatctca aactgaaatc atctgcaaag ataaaggtat
    45061 tgttattcag cagacacagc ataatttccc acagaatata actggggata gaatgttact
    45121 ggaacgagca ttgatgaatt tggtttcaaa ttctttagag tactcgcctg aaaactctac
    45181 attatatata gatgtagata gcaaggacaa ttacttgaat atctgtatta cagactgtgg
    45241 ctgtggcttt tcgcaagagg cattggatca cgcaaaagaa cgtttctata tggcagacca
    45301 aagtaggagc tctaaactgc attatggaat gggtttatat attgttgata tcattataaa
    45361 acagcataaa ggaaaacttc tgctagataa ttccacagaa accaaaggtg ccaaagttac
    45421 tataaagtta ccattataag agaacggctt atttgtcgtt ctttttttca tatctttaag
    45481 aaatcttcaa aattaggttg tattctttgt ttgaaggtaa tggttatatg ctattatacg
    45541 ggggattgtg tatgaagtgt tttaagcaat ttaccaaaaa ctcaagaaca acttaacagc
    45601 aatccatgcg ctaatacaac tgaatatttt aatatcttag ctcgtacaga ctatatgttt
    45661 tgtgcgggct ttttttgtac ccgaaaaaat tttctaactt ttttcggctt cagcttagca
    45721 aatcacacct tgctgtacag atatggtgcg gaaagaggga taaacacttt tcaagtcata
    45781 gctggaaagg gggtgagatt atgaagctgt ctgatttcca gaaaacaata caatgtcaat
    45841 ttgaatgtaa gttaaagaaa gttacaaaag gcattgtaag aaattatcag aaagaattaa
    45901 aacggagaaa aaataaggaa attccttttt gtgaacttcc agaactagtg attgaaaaat
    45961 tatccgtttt agacgattac gacagcgact atacattatt caatgtatgt ggcaacgata
    46021 ttagtattct tgatgatgaa ttggcagaag ccttgaaaaa gctgtctgac cgtaaccgtg
    46081 agaacttact catgtactac ttcttagaaa tgagcgatac cgagattgct aaattgcaga
    46141 atatttccag aagtggagta tttcaaaatc ggtataactc attggaactt atgaaaaaaa
    46201 tactaaaagg agaaaagtaa gaaatgaagc acatttttaa gaaaccgtca ttttatctga
    46261 tttcgtcagc tacggacgga gacgaaaaag cgattgaaaa gatactggca atttatgacc
    46321 cgtacatatc aaaatgttgt ttgcgttcac tctatgatga atacggtaat gtttatattg
    46381 ccgtggatat ggaattaaaa ggacgtatca gagaagccct tatcaaaatg attttggact
    46441 ttgatatacc tttagaaacc gaggaagaat aacaagcagt aaccgcagag catgatttgt
    46501 tcaatcccct ctttcctgct ctgctccctg cttttacatg aaagcaacat gctttcgcca
    46561 cagctctttg acaactgaat aaagcagaca gatacgtttg tgagatagcg agccacgggg
    46621 actgtacgcc acgacctttt caaaagaaag cgagcgaccc acgcagtgat ccgagagcga
    46681 ctgttggaaa agtcgttttg ccacgaccta tcctcacaga ataatgatac gtccgcatgg
    46741 tgcggttctg cccacaagaa tgggaatagt tgagatacta acggagcttt ccaaagccgt
    46801 ctgcctgctt gtaaaaaatg acacacagta aagggggtgc atagattatg aacaatactg
    46861 atgtgcctat ctgggaaaaa tatacgctga cgattgaaga agcgtctaaa tacttccgca
    46921 ttggcgaaaa gaaattgcgt aaattagccg aagaaaatat tgacgctggc tgggttatcg
    46981 tgaacggtaa tcgtattcag attaagcgaa aacaatttga aaaaatcata gatacattgg
    47041 acgaaatcta atgtcattga gccgtagata tggtataata aggtatagcg tatcacggct
    47101 cattccatga cggaaaggag ctttacacta tgtctaatgt aaaacgaaaa gacagtaaaa
    47161 atcgcaattt gcgtaatgga gagagccagc gaaaagacgg aagatacgtt tataaatata
    47221 ccgatatata cggaaagcca cagtttatct attcttggaa acttgtaccg acagacaaga
    47281 cacctgctgg aaagcgtgat gatatatcgt tgagggaaaa agaagcacag ataaaaaaag
    47341 accttaacga cggtatcgac acagtcggcg gtaaaatgac agtctgccag ctctacgaga
    47401 aaaagaacag tcaaagaaag aacatcaaga gggctactga aaaaggacga cagtatctta
    47461 tgaacgctct aaaaaatgac ctgttgggta tgagagcgat tgataccgtc aaacagtcag
    47521 acgctaaaga atgggctatc agaatgagtg aaaaaggata tgcctataaa acgattgata
    47581 actataagcg ttccttgaaa gcgtcatttt atatggcgat acaagacgac tgtatcagaa
    47641 agaacccgtt tgaatttaag ctaagtgatg ttctggaaga tgatacggag cagaaagtta
    47701 tccttacacc agagcaggaa aaacacctgc ttgcctttat ggaaacggac aagatttaca
    47761 gtaagtatta tgatgaggtt gtgcttctgc tggaaacggg acttcgtatt tctgaatttt
    47821 gcggattgac gacgcatatt gatatgcaga acagaatact caatatagac caccagttat
    47881 tgaaagacag cgaaatcggc tactatattg aaacgccaaa gaccaaaaac ggaaaacggg
    47941 aacttccatt gacagaacgg gcttatcaag caatccaaag aatactaaag aacagaggaa
    48001 aggcacaacc gctgattgta ggtggttaca gcaatttcct attcttaaac cgtgagggct
    48061 tgcctaaagt tgcaggaaac tatgagggca tggtgcgagg actaattaag aagtataaca
    48121 agtatcacac ggataagtta ccgaacatca caccacattc attcagacat acttattgta
    48181 cgaatatggc aaatagagga atgaacccaa acaccctgca atatctcatg ggacacgcta
    48241 acataactat gacactgggt tattacgcac acggtacatt tcaatctgca aaagcagaac
    48301 tggaaagact ggcttgttaa tatcggagct tatatttact actcatttac tacttttggt
    48361 tgcattttca tgctggtaaa tgccagctta tgcaaggtat cttccgaaaa gaaaataccg
    48421 ataaagccct aaaatacggg atatatcggc atttaccaac ttatgaaaag ataatcagaa
    48481 atctagtaag tttttaatat aaaaatttta ttaaatttag tatgtaattt tttaatctta
    48541 ttttattcca tctatactct tatgctaagt aataactttg ataatatttc cctaacttat
    48601 gtttcaactg gtttatattg caatttattt aattacagct tatttgcata ataattcgtc
    48661 catatcataa gttgtgtatt tatataaata cacaattaaa aataaatcac tatggtatac
    48721 taattattac gaagactata cttatatgca aagacaaata taaggcatct tatatctttt
    48781 attaattata aatatctttt attaattata aatatctttt atcaattata aaaaattaaa
    48841 cgctatctta tttatcataa attacaaaaa cataaaattt tattactata ttgctataaa
    48901 ttaattgata cacattttac acaataagga gggctatata tgacaaatgc taaaagtaaa
    48961 aaagaatatc taaatcgtat ttgcaaagtg caagactata tagaagaaca tttacatgaa
    49021 cctctttctc ttgatgaact ttctaatata gcaggttttt caaaatttca ttttcataga
    49081 atcttcaaag caatagaaaa agaaactttg gcacaatatg taaataggct aaaactagaa
    49141 aatgcaactg cttttcttat tcatagaact gacatgactg ttactgatat tgcacactac
    49201 tttggcttta ctgattcagc agttttctca agagcattta aaaattacta taaaataagc
    49261 cctataaaat atagaaacaa ttatagcaag aattgcaaag aaccatataa aatttctcag
    49321 tacaatagaa gcatatcaaa acctaaatgt aagaatacta gagaggttaa aggtgaaatt
    49381 gaaattctag aacttgataa tatgaatact atttacacta ggtatactgg ttcatatggt
    49441 aatctaacca gtactttccc aaaactttta gaacggttat tcacatatgc aagtgagcag
    49501 aatctacttg gacttgaaaa cacacaaata ttagccattt atcatgataa tccacaattt
    49561 acacaggagc atcaattaag gactagtatt tgcatgacca ttccagataa cattgttgtc
    49621 aaagagaatg acaatcttgg caatatgata ataccttctg gaaaatatct tgtgggacat
    49681 tttgatattt caaaatctga atatagtgat gcgtgggatt ttatgtataa tgaatggcta
    49741 tcaaatggta attataagct acgtgcctcc ttcccttttg aagtatatct taacaaccca
    49801 cacaatcacc ctcaaaatag acacttggtc gatatatatt tacctataga acctttttaa
    49861 taacctcacc taatctattc ttgtattaag gattaatata tccctatttt acggtttgat
    49921 aaaattgata aaatgaggtg aaccaatgaa agaaataaaa attaatggag caaagataca
    49981 caatcttaaa aatatagatg tttctatccc caaagataag cttgttgttg caacaggtgt
    50041 cagtggctca ggtaagtcta gccttatgtt tgacattgta tttgaggaag ggcgtaaaca
    50101 atatctgcaa tctttaggta tacttgcagg tattgatagt gaagataagt ttgataatat
    50161 tcaaggttta gctcccacta tagctgttca acaaaatatt attcgccaga gcaatcctcg
    50221 ttcaacagta ggcacaagaa ccaatattct caacatgctt acactattgt attcagtgga
    50281 aggtcaaata atgtgtacta tgtgtgatac tcctgtaagc tataatctaa tttgcaataa
    50341 ttgtggatat gaggaagaac gattaagacc aagttatttc tcatacaact cttcagatgg
    50401 aatgtgtatg aagtgctctg gacatggtgc atattttgag attaatattg aaaaattagt
    50461 ccttgataag catgataccc taaaacaagt cttagataga gcaaagatta cacctggata
    50521 tttgagagta ttttctaaaa aattcaatga ttatttagac atacaattct tacaactacc
    50581 tgaagaagta agaaatgaag tactctatgg tcattatgaa aatggtaaaa aaagttcctc
    50641 tttatcaaag gtatttcaca atcgttacaa aaggggcgaa gatttaaatg gagtttatac
    50701 tatgactgcc tgttcagatt gcaatggttt taaaattggt gaagaagcac gtagagtact
    50761 tttaaacgga aaacatattg gagaacttgg aaaaatgacg attttagaaa ttgatgattt
    50821 ccttaaactc ttaataaatc aaggtaattt aaacacattt ggtacaaatc ttgtaaatga
    50881 tattttatcc aaaactcaac atttaataga atcacgatta ggtcatctat ccttataccg
    50941 agaaatgtca actttgagtg gtggggaaat gcaaagacta tttttaaatt cacatctcga
    51001 ttcagagatg gactcattaa tttatgtgct tgatgaacca acagctggat tgcatgaatc
    51061 agaaaaaata gacatcttaa aatcaataaa gaaacttaaa gatttaggca atacggtcat
    51121 agtagttgag catgataaaa acacaattaa aatggcagaa catattattg atattggtcc
    51181 aaaagctggt gttgaaggtg gtcaattagt atatcaagga gatttagaag gtctacttca
    51241 atccgacaaa tcactcactg gacagtatct atctggaaaa tatccaatgc ctaatagaac
    51301 aagcaataaa aacattacac atactgataa aataccttgt ataactattc aaaatgcaaa
    51361 tacaaataat cttaaaaatg taactgtatc attacctttg ggtgctatgg ttggaatatc
    51421 tggaaaatca ggtagtggca agagttcact tatatcagac accctacttc ctcttcttag
    51481 aagccaattt aataaccata ctatcaataa tcaaataaat tcttcagaga gtgaaatagg
    51541 tgaggaagac gattatgcag ttgttgaaac tattgcagat agactgattg gtactaaaca
    51601 tatttctgga tattcagaga tatctcaatc tcctattggc aaaaatatga actcaactcc
    51661 tgcttcttat attggtatat gggataagat acgtacacta tttgcaggtc agccagaatc
    51721 gttagaacga ggatttacag ctggtcattt ctcatttaac tccaaagggg cttgtcctaa
    51781 atgtagtggt agtggatatg aaaaggtctg gttgggaaat aatctaagta tagaccatat
    51841 atgcagtgaa tgtcagggaa aaagatttaa tgatgaatct ttatctatta aatataaaaa
    51901 taaaaatata catgatattt taaatatgag tgtttctgaa tctgtacatt tcttcaagga
    51961 tattccaaat attgtttcac acttaaatgt attagaacaa ataggtatgg ggtatataaa
    52021 attaggccaa cctacaccaa ctttaagtgg tggtgaatcc caaagaatca aacttgcaaa
    52081 agaaattggt aaaaaacgaa atggtaatat cctttatgta ttggatgagc caactactgg
    52141 tctaagtcta tacgatactt ctaagttgat tcaattatta gatgaattgg ttgaaaatgg
    52201 aaattctgta attgttgttg aacatgatat agatgtatta aatgtttgtg actggattgt
    52261 tgagcttggt ccagaaggtg gtgacaaagg aggttatata attgcagaag gttctccaaa
    52321 aactctaaaa gaaaatccaa aatctataac agggaggtat ctttaatgaa taaaaacaat
    52381 tgcttagatt taatatttga aaaagtaaat ccttcaagtg ttggcatgag ttcagaaaaa
    52441 ctctcacaac ttgaaccttt aataatctca caatatagta atataaatgg aataattatt
    52501 gcaagaaaag gaaaagttgt ctttgaaaag tactataatg gatataatgc tgatgatact
    52561 tttcatgttg catcagttac aaaaagtatt atatcagcct taataggtat tgcaatagat
    52621 aaaggatata taaaagatgt tgaccaaaag gttgttgatt ttttccctga atatacttat
    52681 agtccttctg aaatatgtaa aaaagcagta actatacgtc acctacttac aatgactgca
    52741 ccataccctt ttaaaaatat gactgagtct ttagaaagac tttgcaaaca aaaagattgg
    52801 ataaaatata ccttgaatca acttggtcaa aaaggcgaaa ttggtacttt caaatattct
    52861 tcagctggaa cgcacctact ttcagctatt ataacaaaaa ctactggaaa atctgctcgt
    52921 gaatttgcta atgaatactt atttaaacct attggtatga aagttatacc agattatgat
    52981 atgaaagatt ttggttttga agatttcttt ggaaaaaatc ttaagggttg ggcaaaagat
    53041 agacagggtt attctatagg tggctgggga cttacattga gtgctagaga tatgttgcga
    53101 tttggttttc tgtatttaaa tgatggtatt tggaataata aacgtattat atctaaatcg
    53161 tggattgaag aatcagttaa aatgaatagt aatgaatacg gttatctgtg gtggctatgt
    53221 aaaaaagatg gtgttttgtg gtattcagca atgggagctg gaggaaatgc tatttgttgt
    53281 attccacaat atgacttagt aatagccata ccatctaaaa tagtgtttaa acctcgtgat
    53341 agatgggagt ttattgaaaa atatatactt acttctataa tagattaatt actctttcat
    53401 aaattaatta ctcttagata accaaaccct ccattataac accagacttt tgtttggtgt
    53461 tattatgact aatactatat atctaaaagg agttgaatac taatggacac ttacacgaca
    53521 tcagaggtag cagatttaat tggtgtacat cctaatacag tacgtttata tgaagaacta
    53581 aacttaatac ctaaacctaa gcgtaatacc aatggttatc gtatctttaa tgattttcat
    53641 atagaacaat ttagatttgc tcgtacagcc ttgaaaattg aagtggtaca aaggggatta
    53701 agaaaagatg cccttgagat tgtaagaaca tcagcgaata gggactttga taaggctatt
    53761 agtcttgtaa atcaatactt gcaacaatta gaaatagaaa aatctaatgc cgaggaagca
    53821 cttactattg ttgcacagat tttaaatgga aatactccag aaacaaataa actcttttta
    53881 actagaaaag aaactgcaga acacttaaac gttacaatag atactttaag aaattgggaa
    53941 atgaatggtt tgttaaaagt aaaaagaaaa caaaatggtt atcgtgtata tactgatgaa
    54001 gatataaaac gacttaaaat tataagcgca cttcgttgtg caaactattc tctttcagct
    54061 attttacgaa tgttaaatgc tttatcttct aatcctaata ctaatattaa aactgtcctt
    54121 gacactccaa aagatgatga cgaaattatc tcagtgtgtg acaaactgat aacttcattg
    54181 aaagaagctg aacataatgc aaaaaccatg aaatctcatc ttcaaattat gaaaaaacaa
    54241 ttttcctagc cctccactct aactccagac tttgatttgt gtgttatcct taagagcaag
    54301 aaaaattcaa cacacaaatc aaaggaggaa taggatggaa aacaaaaata ctaattctac
    54361 atggaagaaa aaagtcattc tttttttagc aagtcaatat atttccttat ttggctcatc
    54421 acttgtacaa atggcaatga tatggtatgt cacacttcag acatcatcag gtgtatgggt
    54481 aacagtttta actgtatgct cctttatacc tcaaatgata atttcttttt ttgctggtgt
    54541 atgggcagac tactacaatc gaaaggtact cataattgta tcagattcaa caattgctat
    54601 atctacatta gttctaattc tatttatgat gtcatcaaat ggcacaacaa gtgaattaat
    54661 agccatatca gtaatatctg ttatacgttc actaggaagt ggtgtacaaa cacctgctgt
    54721 aaacgcaatg attcctcaac ttgtaccaga agaacattta atgcgtttta atggtataaa
    54781 tagtagccta caatctatag tacaatttat agttcctatt gtagctgctg caatacttgg
    54841 atttggtaat aaaataaatc aagttctttt aattgatgta tttactgcaa ttattgctat
    54901 tgtattattg atattcatca aaattcctaa acatgatata ccaaaacaaa ctgaaaaagt
    54961 ctcatttttt gcagatatgg tatcagggat aaagtactgt ttttcagata agttcattag
    55021 aaaagtgctt tttacttatg gagcattcat gcttctttgt gttccatcag gatttcttat
    55081 aggacttctt attgaacgaa catttggcaa gggttatata tacctgtcta ttattgaaat
    55141 gattggattt ttaggtatgg ttttaggtgg attgattata ggagtttttg gtggttttaa
    55201 aaatagaaat aagacctttt ttctagcaat attatcctat gctatttttt caattattct
    55261 aggtcttgta acacaagctt ggcaattttt tatatttatg ttttttacga atttttcaat
    55321 tccaatcatt caagcatcag ctatgactat gttgcaagag catgtagcac cacaaatgct
    55381 aggtcgagtt cttagcttac ctattattat atatactggt tttattcctc ttggtatgat
    55441 gattttcgga ccaatggcag atgtaatttc tattaataat ttaataatca tagcagggat
    55501 tatactttta ttttttgctt tggctatccc ctattcaaaa caattttaca agcaaggaat
    55561 atctaagtca aatgaatccg agtaatatat ttaaatagtt taactcgatt tatctggtta
    55621 aaaatgaggg ggcttaaaat ggacttttta atttatgaag aaaatttttt atatgaaatc
    55681 aaatatattt tatcatttca acattgaaaa ataggtttat ctttattttg agcccctctc
    55741 tttatagcaa ataatatttt gatactatct ttattaactt tatataataa tacctttact
    55801 tgtaggttat aatgatgaaa tttatcaaat catataataa tacctccatt tatatcataa
    55861 attaaattta tagtacaaat ggaggaaaat aaagtattta tatagtttaa gtacctatta
    55921 agttttccac taagtagctc taattgtact gaacttctta ctaaattata ttgaataatt
    55981 ataaaataaa actaactatt acactacata aattaaagta tacaaattgc actacataaa
    56041 ttacactata caaagtatat taaatcacct attaaattgt cccaattata attatactga
    56101 gttatctact aaaaaataac tttctttttt tatctaaaat aatatatatc ataaaccata
    56161 ctacacaatg tacagccaaa attacaatat ctttgataat aatgacatta ccattaataa
    56221 tctccataat accattaaat gtagcaactg aaggcattat atagtataaa ttccctatac
    56281 cactggtatt aacccctaga agatatatta acaaaggagt acctataaat aaaatcatta
    56341 ctattttaat ataaacaatt cctaccataa gaccttctga caactttcca ataaataaac
    56401 ctataagtga tgaaataaat gctgaaaata caacgagtaa caacatcatt atcatatcta
    56461 atattgaaat atccaaacat ataaatacag ttatcattga agataaacac ccacataaaa
    56521 gacccaaaaa tactttttgt ataatatact gacttctact ctgtggcaaa acttcattta
    56581 taaatgatat tccatcttct ttttcagata taatattcat tgcattaaag gtgcatccca
    56641 taaacatagc agtaattaaa atcatggcaa taaaaacatt ttgaaactct tttaaaatac
    56701 tatctttagg caatattgta acctcaacat cttttaaatc accctttagt ctaaacatct
    56761 ctggcaatgt ctttccaaca ttttttatag cattaatctc atttccataa agcacagttt
    56821 ttatcccttt accatctcta acaactccaa ttgtgtctgt tttaatatcc ttaatttcac
    56881 gtattaaatg catttttgtg tcatatacac ttaccatacc atacttcttc aaccactgct
    56941 ctgtttgtac aggaagtgta tccttcacta taccaaaacg tatctctcct aatgaagata
    57001 tatcaattgt tcccataaaa tttattaaaa tcgccataac aactggtaaa aagaaggaaa
    57061 ttatacacat tttatctttt tttatactct ttaactggta acgtaatcct atcaagatta
    57121 tccctcccta accaactctt tattaaaaat atattttaca agtaaaaata gaactattat
    57181 tgtcaaagaa cacactacaa aataaactaa ttcttttgaa aaattcatga aaaacgcatt
    57241 tttaagctga ctataaacat gatagaaggg atgatattta atccaatcta tttcaaaaga
    57301 tgtatttgca gatagaaata taggtgtagt tatgaataca gcaaccacaa gatatataag
    57361 agaaaattgc ttaaaatctc tcaataacat tgagcatcct attccaatca aagacataat
    57421 aattgataaa atactagctt gaactaatac ttgtggaagt atttttattc cttcaaaaaa
    57481 tccaatatta aatactacta aaagaactgt aaaacatagg tcacatatca aaaaagcaaa
    57541 tatctttgat aatataaaaa gacttctttt tacaggtaaa ataccagtaa tacgcattac
    57601 tcccatctgc ttttctttaa ataataaaga ggcaatccct aaaaatccaa tagctacaat
    57661 ttcaaagaat aaaatctcac aagtcatgtc ttttctttgt tttatctcag atgtatcctt
    57721 atcattaatt tgaattattg cattgttgtc tgatattttg ctattgtggt ctttttctga
    57781 tgataataat gacaatgcat agtcagctct ataatcgtca actcctttaa tactttcatg
    57841 taaaattact ttcggcttac tggaactcat atcaatacaa actgcacttg tatcttcatc
    57901 aagttttgct tttaattcac ctatagaaga aacttgatta attaatggag atgcactcat
    57961 gcttatattc tctggattat atagatatac attattgtga taactatctt catccataaa
    58021 ctttacataa ccaaaattaa tataacacga ataaagtatg agagagccaa gtgtcagcac
    58081 aaaaaattta ccataaatca tcatccaaaa atcttttttt actaaggtaa aaaatttaga
    58141 gtacattaca ctaacatcct tcctgtgtat ttaataaaaa cttcttctag tgtaggttct
    58201 tttgaatgta cacttaagat ttcatcatat ttaaaagtta ttccagattc taactcttgt
    58261 atatctacaa tttcaatatc tcgatttcca ttatatacaa attccacttc aactttatta
    58321 ttgctatttt tcatttttaa atttttgggt ttatctatta ccataatctt tccatctgca
    58381 ataaaagcca cattgttaca cattaaatct gcatctagca tattatgagt agtcaaaaag
    58441 atagttgttc ctttttttcg ttcttcttca ataatcttac gaaatagtac agctccagat
    58501 gggtcaagtc cactagtagg ctcatctaaa aataacaact ttggattact taccaatgct
    58561 cttgccatac taatacgttg cttcatacct tttgagtagg atgatacagg cttatttata
    58621 aactctttct taaactctaa caaatctaag agttcatata tatctctcct ttgattttta
    58681 ggataaaaag acgaaaaata ttctaaatta tcaattgcac ttaaattggt atataaataa
    58741 ggaaattcaa ataaaacacc aatcttttga tataaagatt ctgtgtatgc tttaacttcc
    58801 ttaccaaaaa gggatacgtg accattgtaa ccttttaaaa caccatttaa gattttctgt
    58861 gttgtggatt ttcctgaacc attaggtcca aggaatccaa agatttctcc ttcttttact
    58921 tcaaaattaa gtccatgcaa tatctgttta tcttttccat aagaaaattt taactctcct
    58981 acttttatca tctatcataa cctcctttat catttttttg tcgtctatcc caccatttta
    59041 taaatttaat tttaagcgga atagaaagca atattatcaa tataatagtc aaccatgtat
    59101 accatttcat aagtactccc ccttgtaacc ttatttaatt acgcttctat tttttaattt
    59161 atttaaccta ttttatttct atttggtgaa atcagagtgt ctctcttgtg aaatcgaagt
    59221 gaaataataa aaagcttaat aaaaaatctt gttttcatca gaaaaacaag acttttcatt
    59281 aagcttttta ttagactttt tcattaagct tttttcatta agttttttta ttttattctc
    59341 tttaaatgct tacatataca aagaaaaata ttttacatca gatacttata atttataaat
    59401 taatactaaa ttattcatca attggaatca taaaataaaa ctctactcct ctatcacgat
    59461 tattcactcc atattctaac ccttgcattg taagtatttg tgaaacaatt gcaagcccta
    59521 gacctgaacc tcctgtacgc tgagattgaa cactacgata aaacttagac caaatctttg
    59581 aaatctcatc ttgctctata aacctaccat cattataaat ttcaaataaa agcaagccat
    59641 tttcacatgt aatcgataaa tctaaattac cattatatac aacatgcttc tttgcattaa
    59701 taactagatt ctctactact tgttccattc tcattttatc tgccactaca aaaacttcat
    59761 actctggcaa atcatatgta aaataaaaat tggcatctgg tatatcaatt aaaagacgac
    59821 ctgccacagt ttcaacaaac tctataaaat caaaacgctc tgggttaagt ttagaaactc
    59881 ctgcttccaa tgcagataag tctaataatg atacaatcat attgttcatt ctatcagttt
    59941 cttctattat aacattcata taatttttct ttttttcttc atcaacttca tccataagcc
    60001 cctctgtata cgcacgtata atgccaagtg gagtcttcat ttcatgtgaa attgtatcta
    60061 ccaactcctt tctttgttcc aatagcattc tttccttatt tacatccttt tcaagttgaa
    60121 tatttgcact ttctaggtct gataatgcct gttgtagatt tgtagacatt gtattcaggc
    60181 tcctagaaag ttctccaaat tcatcattag tatttacagt acatggatta gaaaaatcaa
    60241 gttgggacat actttttgca gatttattta tagatgtgat tggttctgaa ataaattttc
    60301 ccaataagaa atcaatactc ataataaata taaccagtat tataaaccaa atccaaaatt
    60361 ctaatttaga gtttatcgaa aattcttcaa caaccagata aaataatata attaacgctc
    60421 ctgataattt agaaagtaaa aacacttttt ttctaactga atatagtcca aaattttgtt
    60481 taatccaatt tgaaatatct acatttttca gtttttctat aatctttctt aaactattca
    60541 ttccttaacc tcaaacttat atcctgaacg aattaatgta actatatggt gtccttcact
    60601 accaagcttt ttgcgtaagg tttttatatg tgtatctaca gttcgtgtgt ttccttcaaa
    60661 atcatatccc catatacaat ttaaaagttt ctctctggaa aaaacttgat tagggttttc
    60721 cataaataaa cgcaataatt caaattcttt atatgttaaa tcaatcaatt tatcttctac
    60781 atacacttgc cttgatgcta ccataataaa aatctttcct aaactcaatg aatttttggg
    60841 aatatcagta gtgtatctac gtaacaaggc attaactttt gctaaaagta ctttggggct
    60901 aaatggtttt gttatataat catctgcgcc atattcataa ccctttaatt tgtcctcctc
    60961 ttcaccttta gctgtcaata taataatagg tatattagtc atttcacgtg aaactctaca
    61021 cactgaaaat ccatctaaat acggcatcat aatatccaat atcattaaat ctatattgtt
    61081 gctctttaat attgtaagtg cctcgactcc atcatcagca gttatacaag tatatcctcc
    61141 cttttgcata tattctacaa taatactttg catccttttt tcatcttcta caattagtat
    61201 tttagccatt tctatttatc acctcttttt tgtaggcttt attttagttt atattaattt
    61261 atttattttt caactatttt tttatagtat tttgatagta ttttttatag cattttgata
    61321 gtatttttta gactattctt aatggaattg atatcataga taaaaatagc ctactaactt
    61381 tttctatgtt agcaggctaa aatatttcta ataataatta tattattttg tcactattct
    61441 attttttcga ttaaaatata ttctaaaaca aatatcttca attaatgaat aaattgcact
    61501 tgttataaag tacagaccta tggctactgg agttctaaca gttagtataa gacttgttat
    61561 ggtagtcacc acagccattt gtttgttaaa ctttacttga gaatttattt ttaaatatgg
    61621 tatataattc actaaatttg aagctaacat aattaatgta tatatacaag gtaatataaa
    61681 caaattatca aatgtaccta aattagcaat ccatggaatt ataattgttg taaattcctt
    61741 tgtcatatct aaacatacat gatataaagc atatactact ggcatttgaa ccaaaactat
    61801 aagacatccc agcatactct tcatactttc tacagaactt acttgtattt gcttttccaa
    61861 ttctttagta tcattcttat acttttcttt tatatgttcc attttttcag ctaattcttg
    61921 ctgttttttt acagaaaatc tttgttttaa tgatagtggc ataagtacta atttaactat
    61981 taaagttata agtacaatag aaattccaaa atcacctgta aaattaaata atatacttaa
    62041 taatccttgc aatatacttg aaatgaaatc cattttattc tctccttttt atttttaaac
    62101 tttagaattt ataatagatt catttcaaat taaaacaaac tacaatacct ccaaatatca
    62161 tatttctgaa aaactatata ctgtttaggc aaccgttctt cagaatatgt aattctattt
    62221 acaaatatgt ctcttattaa aacatactta attttatgat ttaagaggtc tttacaaaaa
    62281 taaaatatac ttccaatgat agataccaat aatgtcatat aaattagtac atataacata
    62341 ctatccatat ctaatctcat cacatcacct cttcatcact tatatttatt tttagacaat
    62401 ttcaagtcct aaataattcc aacatttata tttagtttta aataattata g
//
LOCUS       DAMJYX010000013        60494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJYX010000013 DAMJYX010000000
VERSION     DAMJYX010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.013"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            18..1298
                     /locus_tag="PC479_000880"
     CDS             18..1298
                     /locus_tag="PC479_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2803461.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1561..2952)
                     /locus_tag="PC479_000881"
     CDS             complement(1561..2952)
                     /locus_tag="PC479_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2803462.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2963..4183)
                     /locus_tag="PC479_000882"
     CDS             complement(2963..4183)
                     /locus_tag="PC479_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2803463.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4197..4985)
                     /locus_tag="PC479_000883"
     CDS             complement(4197..4985)
                     /locus_tag="PC479_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2803464.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5002..6276)
                     /gene="larA"
                     /locus_tag="PC479_000884"
     CDS             complement(5002..6276)
                     /gene="larA"
                     /locus_tag="PC479_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2803465.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6394..7107)
                     /locus_tag="PC479_000885"
     CDS             complement(6394..7107)
                     /locus_tag="PC479_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2803466.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7232..8491
                     /locus_tag="PC479_000886"
     CDS             7232..8491
                     /locus_tag="PC479_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2803467.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8771..9337
                     /locus_tag="PC479_000887"
     CDS             8771..9337
                     /locus_tag="PC479_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2803468.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9538..10743)
                     /locus_tag="PC479_000888"
     CDS             complement(9538..10743)
                     /locus_tag="PC479_000888"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2803469.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10846..10952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11044..11412)
                     /locus_tag="PC479_000889"
     CDS             complement(11044..11412)
                     /locus_tag="PC479_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2803470.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11464..12741)
                     /gene="obgE"
                     /locus_tag="PC479_000890"
     CDS             complement(11464..12741)
                     /gene="obgE"
                     /locus_tag="PC479_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2803471.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12852..13142)
                     /gene="rpmA"
                     /locus_tag="PC479_000891"
     CDS             complement(12852..13142)
                     /gene="rpmA"
                     /locus_tag="PC479_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2803472.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13156..13473)
                     /locus_tag="PC479_000892"
     CDS             complement(13156..13473)
                     /locus_tag="PC479_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2803473.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13488..13799)
                     /gene="rplU"
                     /locus_tag="PC479_000893"
     CDS             complement(13488..13799)
                     /gene="rplU"
                     /locus_tag="PC479_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2803474.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13900..15321)
                     /locus_tag="PC479_000894"
     CDS             complement(13900..15321)
                     /locus_tag="PC479_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2803475.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15339..16040)
                     /locus_tag="PC479_000895"
     CDS             complement(15339..16040)
                     /locus_tag="PC479_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2803476.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16042..17886)
                     /locus_tag="PC479_000896"
     CDS             complement(16042..17886)
                     /locus_tag="PC479_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2803477.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18205..19398
                     /locus_tag="PC479_000897"
     CDS             18205..19398
                     /locus_tag="PC479_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2803478.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19610..20430)
                     /locus_tag="PC479_000898"
                     /pseudo
     CDS             complement(19610..20430)
                     /locus_tag="PC479_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(20560..21351)
                     /locus_tag="PC479_000899"
     CDS             complement(20560..21351)
                     /locus_tag="PC479_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803479.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(21355..21684)
                     /locus_tag="PC479_000900"
     CDS             complement(21355..21684)
                     /locus_tag="PC479_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2803480.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22169..22582)
                     /locus_tag="PC479_000901"
     CDS             complement(22169..22582)
                     /locus_tag="PC479_000901"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2803481.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(22623..23753)
                     /gene="rodA"
                     /locus_tag="PC479_000902"
     CDS             complement(22623..23753)
                     /gene="rodA"
                     /locus_tag="PC479_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2803482.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(23838..24122)
                     /gene="minE"
                     /locus_tag="PC479_000903"
     CDS             complement(23838..24122)
                     /gene="minE"
                     /locus_tag="PC479_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2803483.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24140..24937)
                     /gene="minD"
                     /locus_tag="PC479_000904"
     CDS             complement(24140..24937)
                     /gene="minD"
                     /locus_tag="PC479_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2803484.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(24961..25644)
                     /locus_tag="PC479_000905"
     CDS             complement(24961..25644)
                     /locus_tag="PC479_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2803485.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(25742..28720)
                     /locus_tag="PC479_000906"
     CDS             complement(25742..28720)
                     /locus_tag="PC479_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2803486.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(28732..29220)
                     /gene="mreD"
                     /locus_tag="PC479_000907"
     CDS             complement(28732..29220)
                     /gene="mreD"
                     /locus_tag="PC479_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2803487.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29235..30128)
                     /gene="mreC"
                     /locus_tag="PC479_000908"
     CDS             complement(29235..30128)
                     /gene="mreC"
                     /locus_tag="PC479_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2803488.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30138..31148)
                     /locus_tag="PC479_000909"
     CDS             complement(30138..31148)
                     /locus_tag="PC479_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2803489.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31269..31928)
                     /gene="radC"
                     /locus_tag="PC479_000910"
     CDS             complement(31269..31928)
                     /gene="radC"
                     /locus_tag="PC479_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2803490.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(31973..32557)
                     /gene="maf"
                     /locus_tag="PC479_000911"
     CDS             complement(31973..32557)
                     /gene="maf"
                     /locus_tag="PC479_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2803491.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(32777..33748)
                     /locus_tag="PC479_000912"
     CDS             complement(32777..33748)
                     /locus_tag="PC479_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2803492.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(33764..34339)
                     /gene="rsxA"
                     /locus_tag="PC479_000913"
     CDS             complement(33764..34339)
                     /gene="rsxA"
                     /locus_tag="PC479_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2803493.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(34354..34947)
                     /locus_tag="PC479_000914"
     CDS             complement(34354..34947)
                     /locus_tag="PC479_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2803494.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(34960..35529)
                     /locus_tag="PC479_000915"
     CDS             complement(34960..35529)
                     /locus_tag="PC479_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2803495.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(35529..36506)
                     /locus_tag="PC479_000916"
     CDS             complement(35529..36506)
                     /locus_tag="PC479_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2803496.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(36529..37866)
                     /gene="rsxC"
                     /locus_tag="PC479_000917"
     CDS             complement(36529..37866)
                     /gene="rsxC"
                     /locus_tag="PC479_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2803497.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38082..39026)
                     /locus_tag="PC479_000918"
     CDS             complement(38082..39026)
                     /locus_tag="PC479_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803498.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39137..40411)
                     /locus_tag="PC479_000919"
     CDS             complement(39137..40411)
                     /locus_tag="PC479_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2803499.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(40543..40914)
                     /locus_tag="PC479_000920"
     CDS             complement(40543..40914)
                     /locus_tag="PC479_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2803500.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41142..41741)
                     /locus_tag="PC479_000921"
     CDS             complement(41142..41741)
                     /locus_tag="PC479_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803501.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(41942..42154)
                     /locus_tag="PC479_000922"
     CDS             complement(41942..42154)
                     /locus_tag="PC479_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2803502.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(42227..43942)
                     /locus_tag="PC479_000923"
     CDS             complement(42227..43942)
                     /locus_tag="PC479_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2803503.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(43942..44496)
                     /locus_tag="PC479_000924"
     CDS             complement(43942..44496)
                     /locus_tag="PC479_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2803504.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(44489..45091)
                     /locus_tag="PC479_000925"
     CDS             complement(44489..45091)
                     /locus_tag="PC479_000925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2803505.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45100..47748)
                     /gene="polA"
                     /locus_tag="PC479_000926"
     CDS             complement(45100..47748)
                     /gene="polA"
                     /locus_tag="PC479_000926"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2803506.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            47900..47987
                     /locus_tag="PC479_000927"
     tRNA            47900..47987
                     /locus_tag="PC479_000927"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47934..47936,aa:Leu,seq:caa)
     gene            complement(48174..48617)
                     /locus_tag="PC479_000928"
     CDS             complement(48174..48617)
                     /locus_tag="PC479_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803507.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(48620..48817)
                     /locus_tag="PC479_000929"
     CDS             complement(48620..48817)
                     /locus_tag="PC479_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2803508.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(49009..49875)
                     /locus_tag="PC479_000930"
     CDS             complement(49009..49875)
                     /locus_tag="PC479_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2803509.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(50101..50970)
                     /locus_tag="PC479_000931"
     CDS             complement(50101..50970)
                     /locus_tag="PC479_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2803510.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(51476..51697)
                     /locus_tag="PC479_000932"
     CDS             complement(51476..51697)
                     /locus_tag="PC479_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803511.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(52198..53178)
                     /locus_tag="PC479_000933"
     CDS             complement(52198..53178)
                     /locus_tag="PC479_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803512.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(53342..54214)
                     /locus_tag="PC479_000934"
     CDS             complement(53342..54214)
                     /locus_tag="PC479_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2803513.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(54378..54929)
                     /locus_tag="PC479_000935"
     CDS             complement(54378..54929)
                     /locus_tag="PC479_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2803514.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(54949..55851)
                     /locus_tag="PC479_000936"
     CDS             complement(54949..55851)
                     /locus_tag="PC479_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2803515.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(55866..58238)
                     /locus_tag="PC479_000937"
     CDS             complement(55866..58238)
                     /locus_tag="PC479_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2803516.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(58459..59148)
                     /locus_tag="PC479_000938"
     CDS             complement(58459..59148)
                     /locus_tag="PC479_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2803517.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(59389..>60494)
                     /locus_tag="PC479_000939"
     CDS             complement(59389..>60494)
                     /locus_tag="PC479_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2803518.1"
                     /translation="SIFFYLFSVYYKCLDAVIYRILFFAITGNVISSKESLLVPYLES
                     IKIQIYSNLIIILIYLIFSFFRKSIKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKL
                     ERFGSLHSEGYYFDNFVNPKLVGASSIFLILLFKEIIKENRLKSQAELIKNKLDMQYA
                     HYLSIQESHMRVKKLYHDINNHIYCIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGN
                     MILDIIINEKSEVCLKKGIKFTCSINFSKVNFVKPIDVSSIFSNILDNAIEACDKIVD
                     ENINKYIRIKGTITRSFFVLKCENSKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSL
                     QKYNGELLFENSIDKFILNIYIPLDQDTDSWGD"
BASE COUNT        19001 a        11154 c         6466 g        23873 t
ORIGIN      
        1 gataaaagga gtattttatg aacatacttg gattaatagt tgaatataac ccatttcata
       61 atggacacat atatcattta tctaaatcta tagaaaaaac aaatgcaaca catactgtag
      121 caataatgag tggaaatttt ctacaaagag gcgaaccagc tttatttgat aaatacacta
      181 gagcaaaaat tgctgtcgaa aatggtgtag acttagttat agaattacca actttatttg
      241 cttgtcaaag tgctgaaatt ttttctcatg gtgctataac aattttaaat tcactaaact
      301 gcataaattc tatatgtttt ggtagtgaag aaggaaatac agaaattctt tataatctag
      361 ctgaaatttt agcaaatgaa ccaaatgaat ttaagttact tttaaaaaaa tatttagatg
      421 atggtctgtt atttccaaca gctagagcta atgcactttt tgattatata catcaagaca
      481 aaaaaaataa ttttatacaa aattattcaa aggaacaatt atcaaaaaca ttaaattcat
      541 caaacaacat actgggtata gaatacataa aaaacctcat ccatttaaac agtagtataa
      601 aaccatttac tatctccagg gtccaatcag aatataattc cgaagaaatt gagagtgata
      661 tttgttctgc tactgctata agaacttcac ttaaagaaaa tacagacctt tttcatttaa
      721 aaaatgttat tccagaaaaa acttataaca tcctaaatga aaaaatagat aatggttttt
      781 tacctatgtt tgatgacttc ttctttgata ttttaaaaac aattatactt agagatatgg
      841 acactttaaa agagtatttt gaagtaaatg aaggcataga aaacagaatc tataaagata
      901 tctttacatc tgaatttcta catgaactac aattagcaat taaatccaag cgatacactt
      961 taaccaaaat caaacgaact ctaaacaata tattgttagg tattaaaaaa agcgacgtgc
     1021 aacttataaa aaacatagat actatacctt atataagaat acttgctttt aatgataaag
     1081 gtagagaaat tctaaaagag ataaaaaata aatctgaaat aaaaataata aacaagtttt
     1141 caaatatatc attttcatta gatgatacga tttttaaaac actcataagt tatgatataa
     1201 aagctacaaa tatatataat tcaatatatt ataaaaacaa taaagattta gtaaaaggcc
     1261 ctatggattt ttatactcat ccaatatatg taaagtagtt ttttaaaata actatattta
     1321 taaaaatcat tttattaact ctttattata taagatacaa tagtcttact ttatttatgt
     1381 acacatgctt ttaggatgac aacatcaact taaatattat gttactttct aaatttaagt
     1441 aaatattgcc atcctaaaaa ttttacaatt tagcttcaga tttaaaagta aagtttaaaa
     1501 tattatattt aaatctaatt taatattttt tgttttataa atattattaa attaatattt
     1561 ctaatatatt ttccctggat ttaaaatatt tttagggtca aaagctaact ttatattttt
     1621 aattaacatc atataagcat ctgaatttga ttcatgtaag tactcttttt tagcatatcc
     1681 tatgccatgt tcaccagaaa cttgaccact aagttctcta gattttttat acatatagtc
     1741 aaaagtttct tttaatctta ttttccatgt attatcatcc ataccatctt ttaaaatata
     1801 tatgtgtaga tttccatctc ctgcatgacc aaaacttttt attcttattt ttaatttgtc
     1861 ttgaagctcg tgagtgtatc ttataaactc tgctatctta tctcttggaa caacgacatc
     1921 acattcatcc atttgtgtcg tagatgcctt tatagcttct aaaaatgcac ctctggctga
     1981 ccatatagaa tcgtttcttt cttgtgtatc tgatataaat acatctaatg caccattttc
     2041 taaacataaa ttagctactt tttcatattc tttttctata tcttcagtgc tatttccatc
     2101 aaatgtaagc aaaagataag catctgatgt attatctgga aactttttac ctaaaaattc
     2161 ttctgctgcc aatatcacat ctctttccat aaattctata gccgttggta ttgattttga
     2221 ttttattatt ttaggaacag tttctattgc cattgataaa tctggaaatg gtataagcaa
     2281 acttatactt ttctttggta aaggaagcaa ttttaatata gctttagtaa ctattcctaa
     2341 agtaccttct gagcccacta ataaatcctt tatactatat ccagaactat ttttaaccac
     2401 ctttccacct acatttataa tttctccatt tggtagtaca acttcaagtc ctcttacata
     2461 atcccttgta acgccatatt ttactgctct cataccacct gcattagtgt ttatattacc
     2521 tgcaattgta gcactttttt cacctgggtc aggtgggtaa aataaatctc tatcttgaac
     2581 atattgacct attgtcatca ataaaacacc tggttctact gtcaatgtta aattttcata
     2641 atcaacttca agtattttat tcattttaca tagattaatc attattccac catttatggc
     2701 aacagcagca cctacaagac cagtaccttg acctcttggt gtgactggta tattattttt
     2761 atatgcgtat ttcattattt tagatacttg ttcagtttca agtacattta ctaaaacttc
     2821 agggtacttc tcaatccctc caagttcatc atgtgaaaaa tcttcattta tatcacttcc
     2881 aactaataca ttttcttctc cacaagtatc tattaaaaaa tctatatctt ttttatcgat
     2941 taatttatac atttctatcc ccctatttag ctgctgatgc catatatttt tgattattat
     3001 ctttgttatt ttctattttt tcaatcaatt taggaataat atcataaata tcacctatta
     3061 aagctaaatg tgctacatca agtataggcg ctttatcatc tttatttatt gctactatat
     3121 agtcagcacc ctgcatccct gcaacaaatt gaactgctcc agatacacca caagttatta
     3181 tcaatttagg ttttacagtt ctaccactaa gacctatttg ttttttagca tctatccaac
     3241 ctgcttctat tacaggtctt gttcctgcca cttgagcgtt taacttatca gctaatttat
     3301 atatcatctc catatcttct tgttttttta ttgctctact tgccacaact atcacttcag
     3361 cttcttccag accaacttct ttattttttt cttttacact cagtacctta attttagatt
     3421 ttagttcttt tttttctact ttacataaag ttatctttcc tgtagtattc tctatttttt
     3481 ctggtgctga aaatatttta tatcttacag tggcaaattg tggtctatta ttaggtgtgt
     3541 ttatatgagc cattatattt ccaccaaatg ctggtcttat ttggtctaaa tcagtatttg
     3601 attgaatatc aagtattgta caatctgctg taagacctgt tctaaatcta gcggcaagtc
     3661 ttggagctaa tgacctacct aatgtagttc caccaactaa tactatcgtt ggttttattt
     3721 tatctataaa gttttctatt gcttttgaat atggttctat cctaaaatct ttaaattcat
     3781 cttcatcata tacaaatact tcatccaccc cataagaaat caaattactg cacttatctt
     3841 ttatgtcttt tcctacaaat acacaataca caggttgttt tatttttgat gccatctcac
     3901 gagctttacc tataagctcg tacgttacag gatgtatatt tccattatga tgttcaacat
     3961 atactgcaat tcccctccat tcatctttat taatttgtac tttactactc tctataaatt
     4021 caaatgcgcc ttttggtcca cttttgacac acaacttgca catcttacag ccagcactta
     4081 tttctactat acctgacttt tcttctatag ccccaaaagg acatatttct aaaattctct
     4141 ttaagtcagt tattttatct tgatttacaa ctatctttga catttattac tcccccttac
     4201 accaacttta attcctctaa tttatttaca agtgcaaaac ttagttcatc tgcatttcct
     4261 cttacaatat ttttatctgt atttggcttt ggaggaaata ttcttacaac ttgagtagga
     4321 gaaccattaa gtccatagtt attttcatct ttatcttcaa agtcatctaa gctatataca
     4381 ggtatttctc tatctttagt attaagtttt aatttaaatg aaggtagtct tggttgaaaa
     4441 atgtcttttt ctacagttat taaacatgga tattcaatct cacatacttg taaatcattt
     4501 ggcatatcta tttcaacaac tatactttca tctttaactt caattaattt tgtaacgtta
     4561 gtaacatgag gtatatctaa aaattcagct acttcaggtc ccacctgtgc tgtatcacca
     4621 tcagtagttt gttttccaca aattattagg tctacctttc ccatcttctt aataccttgt
     4681 gatatggtat aggaagtagc caatacatct gctcctccaa atctcctatc acttattaat
     4741 gctccttcat cagctcccat cataaaactt tcttcaatta ccttctttgc ctgaggagga
     4801 cccatgctaa gtacttttag attcgctttc ttatcttcct ttattctaag agctgtttct
     4861 aaagcatata ggtcataggg attcattttt gaatctactc catctctttt tagtactcct
     4921 gtagtttcat caacttctac tgaagtcgtt ccaggaactt gtttaataca tgtcaaaatt
     4981 tccataaagt acactccttt tttatttaac tataactgat actccatcag gtataactac
     5041 tactttgctc tccttaccaa ctatactatt cgccatttct aaagcttctt taaagttaaa
     5101 tgcatgtttc atatgcattt tttttatcat ttcaggatta cacttatctg ttaccattat
     5161 cacagtatat ttagataata ttctcgctag tatctgaaat tcccattggt ctggaatcgt
     5221 atctactcta tcgacacttc taattttatc taaaagttgt tttggagaat ctgcatttgc
     5281 tacattttca taaaaagatt gacctccatg accatcatta catgctgaaa tcattattat
     5341 tactccaccg tctttacaag tagcttcagc tgctgtcatt cctttaacag attgataaat
     5401 attttggtct agtggatatc caccatttgt agttacaaca atatcagatt ttatagaatt
     5461 tacttttgat aactctgtta caaatctaca tcctttagta tgtgcttttt cgacatcacc
     5521 agcaaaggaa gcaattattt ttttatcttt atctattaca acattcaata tgaatgctaa
     5581 ttttgcaact ttagctgcat ataccatatc ctcatgaata ggattattat tcaattttcc
     5641 agttcttgaa ttatcactat ctataaagtc actacaatga ttgtacatta tagtctttgc
     5701 agatgcgatt cctggaagta cactttttct gccacctgaa aaacctgcaa agaaatgagg
     5761 ttctataaaa ccttctgcta tcaaaagttc tgcctcatat gctagtttgt ttatatataa
     5821 atctccacct gaaggcaata tccctacttt acacatagat ttatcatcag tagatatatg
     5881 cataactata tcttcattat ctactatttc ttctcccatt ttataaatta gctcttccct
     5941 tgtacttggc ctatgaaatc cagttgccac tataatcttc acatcaattt ctggattaac
     6001 attcctaatc cttcttaata taattggcat agttattcta cttggtacag gtcttgtatg
     6061 gtcacttgta attatgacta cattcttttt ccccttaacc agttcttcca gagaattaga
     6121 gcctatagga ttattcatag attcttcaac tatctcttct tgagtcttat gttctatata
     6181 attatccgat tttgactcca aaacaccgag aaaacgttca tcaggtattt ctaaaatcat
     6241 tccttgtttt gaatacggta cttttaactt taccattttt aatctcccat tataataaac
     6301 tataaaattg gtatgtggtc ataccagttt tacatttata tgatagtacc attatctacc
     6361 atttgcaaga ataatttatt ttataacaaa cttttaattt tcattttttc tataattttt
     6421 acttataaaa tccatatgtt cctttgcttt ctcacatgca agttctacat cattttctac
     6481 aactgcattg tatatagctt catgctgttt aagcaatata ttttcattaa aaggttctgc
     6541 gattattttt tctcttgcat cttctataaa tccatcaaat aattttgatg ctgtcataaa
     6601 aagactttga attaaataat ttttacttaa tgttgctagt ttactgtgaa atttttggtc
     6661 taataacacc agtactcgat tattatcttt tttattagtt tctaccctca tttcatctat
     6721 tatatgtttc aattctataa cttcagaaga agttattctc tttgaagcaa gttttgcaat
     6781 ctcaatttct aggattattc taagctctaa tatatcttca agtgtaccat tattaagttt
     6841 aaaaatcata gataaaggtt ctattaaagt tttttctata tttgtacata taaaatttcc
     6901 ttcaccctgt ctgctttcta tcactcccat agtctctaat accctaatag cttcccttat
     6961 ggaagttcta ctcacattca tttgttcaga cagttctctt tctgatagta atttatcccc
     7021 cttttttaac tctccattta gtattttata ttgtatttgc tcaattactt gttcataaac
     7081 ttttttacta gctatactat taaacatttt ttcctccaat atgataatcg ttgttgtaat
     7141 atatatctat tatatatctt aagccattac attcatatat ttttattact tttaatataa
     7201 tatcttataa ctcaaattta caggtgatat tatgaaaaaa aataaaatta ttgctttttt
     7261 tgctccatct atcatagttg gagttctagt tttagggatt atcctatacc caaatgaagc
     7321 tataaaatct gcaagagaag gcttttctat atggtctaat gttttagttc catcactact
     7381 accatttata attggagcaa acttaattgt tgatttaaaa atagtggata ttatagggtt
     7441 tatcatcaat cccataacta gatttgtatt taatgtttca ggaaagagcg ctttagcttt
     7501 tgctatatct acagtatctg gttatccagt aggagtaagt ttagcttctg aatttaggag
     7561 taatggacaa atttctaagt atgaagcaca aagacttgtg tctttttgtt ctacatcagg
     7621 acctttattc ataataggtt cggttggtac aggtatgttc caaaattcaa acttaggtta
     7681 tttaatgatt ctctgccatt atctaggaac tatattggtt ggtcttttct ttagaaatta
     7741 tggtaatgaa aatcttccaa agacaaaatt ggatttaaaa acaaatataa aaaatgtaat
     7801 caacattaga aattcaagtg ggaatggttt ttttgtttta tttggaaatg ctgtatttaa
     7861 tggagtaaat acattactta cagttggtgg atttgtaatt gttttttcag tagtatttaa
     7921 aatactatct ctttttaatg taatctcatt aatagcatca gttatatatt tgcctctttc
     7981 atttttaggt gttagtaaag aactctgtca agcatttgta agtggtctat ttgaaattac
     8041 tataggatgc aataaagtat ctagtataac aagttctcca gaaattttaa gagcatcttt
     8101 agctagtttt ttgataggat ttagtggatt atccatccta gctcaatgtt gtacatttct
     8161 atcaaaaacg gacatagatt taaaattata catactaagt aaatttgcgc atggagtttt
     8221 agctgctatc tttatattta tactttatcc aattgcaaac tctgctgtgt tggtttctag
     8281 ctttgctgat acatacaacg ttatatataa taatttaatc tggttttatt atcttagcta
     8341 ttatgataca attcttcaaa ttgtagtaat aatttatcta atctcagccg tatttattgc
     8401 aaaaaaacat atgaaaactt tatctacaaa tgggaaacta atgcgatata aaaatacttt
     8461 tttcactaag aaattttcac gaaaaaaata aaactaaaaa taaaactaat aaaacttaac
     8521 taaatacttc cattttttta ttatatttgt cataatttta ataacctttt taattagttt
     8581 aacagcatag ttatttcatc acaatttaat aattttatta gaaatatttt tcaaaaaatt
     8641 ctccttaatc acatatatca cacttgtata agtttactgg gaaatttttc cattatataa
     8701 tatttcattt atattatata ataaataccg acctaaactt accaaatcaa actaagagga
     8761 gtggttgaaa atggcaacaa gacctataga aatagacgaa tataaaaaga taatggaact
     8821 attacatact ggctttactt atagtgaaaa tggcgttgaa aaaagattta gaaaaaatcc
     8881 taaagttgca ttggcactaa tgttagaagc taatttaggt ctaagaatat cagatatttt
     8941 aagattaaag ataggtaatt ttaaaggtaa tatgttggaa ataaatgaaa aaaagacggg
     9001 caaattacag tatagaccag ttaataaaaa tataattgag tccataaatg aacatgcaag
     9061 aaagtataat cttaaatcaa atgactactt agtaaatata aagacaaagg ctattcaaaa
     9121 acagttgagg ataatttgta aatacttaaa tctgtataat ataagtacac atagctttag
     9181 aaagctctat gctactactc aatttgaaaa gagtaacaat aatttagagt tggttaaaga
     9241 acttttaaat cacagctctg tagccactac acagcgatat ataagggtaa ctcagcaagc
     9301 tattaatgaa gctagtgaaa atttctttat tggttaatta taacgtatac atttttctat
     9361 tatcttatta attcaccaga tattaaaaaa gagtgtctca aaatgaactt tttagttcat
     9421 gagacactct ttttttataa aatcaaatat atttttatta tttcaacact ggaaaaaagg
     9481 cttatctctc tattttgaga caccctatta aattttcata attcttgcat aaacatatta
     9541 atctatcttc aataattttt tagctgattc actgtaatta tttcttttta caatttccaa
     9601 aactctatca atctcatgtt ctgactttgc acttacaaaa atcgccaatt cttcatcatc
     9661 tataatatca taactatcta catatttatc taaacattct aaaattccat ctttattatt
     9721 atacccacca aatctaacta ctgcactaca ctcatcttct ataagtccac ttaaatccaa
     9781 tttccctttt gaaaaagact ttatatttga acttctatta tctattatat ctataatatc
     9841 tgcataaaca gcacttcctg ttgcacctct tccagctccc ttacctacaa aagaaagttc
     9901 acctactgaa tcacctacta aaataactac gttgaactca ttatctatct ttgaaagcat
     9961 attattattg tccactaatg ctggtctaac aaatccactt acttctcctg attcatactt
    10021 acttctagca accaatttta tcttacaatt taattttttt gcatattcta tatctttttc
    10081 atctattgaa gttattcctt ctaataataa atctttccaa tatactattt ttccataaga
    10141 taaagtagac aatatagata actttctagc actatcatat cctaaaacat ctgcatctgg
    10201 attaacttct gcaaatccca actcttgtgc ctgttttaaa gcgacatcat acggtaaatt
    10261 ttcatcatac atctttgata atataaaatt acaagtccca tttaaaatag cgtttatgct
    10321 ctttatattg ttgccttcca gtgattctat caatggtttt aaaactggaa taccacctgc
    10381 cacagatgct tcaaacttga tgctaacatt atttaagtta gctaattcta taaggtcact
    10441 acctttttca gccagtaaat ctttattagc tgtcactaca tgtatttttt tatttaacgc
    10501 tcttgttaca taatcaaaag ctggatttaa tcctccaatt acttcaacta gtatatcaat
    10561 atccttgtca aatatatctt ctatatcagt agttatctta tcaaaatact tcttatactt
    10621 gtgtttttct ttatttttaa ctaaaattcc aacaacttct atatctcttt ttgaagtatt
    10681 attttctatt aaatcaatta atccacttcc tactgttcca taccctaata ttgctatctt
    10741 cactgtaaca tacccccatc attttgatta tattaaatta tttatatata taaatatacc
    10801 aataaaaaaa gcttgagtat aaactcaagc catatgacgg gtttatactc atctttcagc
    10861 ataaatgctg caggatttag caccaatgta taattaaata tactggttgc cgggcttcac
    10921 agggccagtc cctctgccac tcttgataag attgctaatt taaatttatg tttaatagta
    10981 taatacaatt tcttccaatt gtcactattt ttttaactta tttccctaaa aggatataaa
    11041 ctattatcta acagctcttt tagttggttt accctttttt gtaacattat tatttttagt
    11101 ctttgctttc gcctgttcat gtcctggaaa aagtatttta ggctcttcaa tattcctctt
    11161 gtaaatagta aatttaaaac caatagcctg cacaaattca gctcttaaag cttcacatac
    11221 tgcattagca gtttctttag tatcaagacc tgcattttct aatatagtaa cttttactaa
    11281 ttctctagtt cttaacatac catctaattg ctctaagaaa ctctctgtaa caccttcttt
    11341 accaatttgt gtagtaggct ttaaagtatt tgccaaagat cttaaatacg ccctttgttt
    11401 tccttttaac atattctcac ctctataatt ctatacttaa attatctata atctatattt
    11461 aaattaattg taaaactcaa attctaattc ataaatttta accacatctc catcttctat
    11521 tcccatttct ctaagcctat caaaaacacc ttgactctcc atagcttttt ggaaatattg
    11581 aagggattcc atatcatcaa aatttacaga atacattatt cttctaagtg ctttacctgt
    11641 aactacatac acaccatctt caatatctat acttaactct tcttcagtac caatatcaag
    11701 ttctggtcta tacatttctt cttctgaaac tagctccaca tcttctactt cttttagcat
    11761 tttagaaaca taggctatta cttcatctat gccttgacga gttgctgctg acattttaaa
    11821 tactgtatat cctctgcctt ctaattcact cttaaacttc tcaaacttac tctcatcttc
    11881 taatatatca aatttatttg caactacaac ttgtggtctc tttgaaagct tttcattgta
    11941 caatttaagc tcatcattta tcttgtcaaa atcttcaata gggtctcttc cctctaatcc
    12001 agatatatct acaatatgta tcaatacctt tgttctctca acatgtctta agaactcatg
    12061 acctaatcct atgccttcag aagcaccctc tataattcca ggtatatctg caagtacaaa
    12121 actatctcca aacttagttt gaacaacacc tagatttggt gtaagtgttg taaaatgata
    12181 gtttgcaatt ttgggtttag ctttagttac aactgataga aatgttgatt tacctacatt
    12241 tggaaatcct aaaagaccaa catctgctat cattttaagt tcaagagtta tccatctttc
    12301 ttctccatct gtaccagatt ttgcaaaagc tggagcttgt cttactgcat ttgcaaagtg
    12361 ttggttacct ttaccaccta taccaccttt tgcaactata gctgtatctc cttctttttt
    12421 taaatctgct aatacaagac ctgtagcctc atctcttatt actgtacctg ctggtacttt
    12481 taatactaaa ttttctccat ttttaccagc tctttttcct ttgcttccgt cgccaccatt
    12541 ttgagcttga tattttttct ggtacttaaa gtccattaga gttctaagcc ccaaatcaac
    12601 ttcaaatatg atgcttgctc cacgaccacc atctccaccg tctggaccac cagctggaac
    12661 gtatttttct cttctaaatg ctaccgaacc atttccacca tttccagcct taacaaatat
    12721 tcttgcctta tctataaaca aattcttcac ctacttaatt aaaaactttt aattagcatt
    12781 accaaaaata ccattataaa ttcatcagta ttataagaaa aaggagtgcc tcaacactcc
    12841 ttttatgtat attactcagc tattgctact ggatatatac taactttttt tctcttcttg
    12901 tcttttcttt cgaatttaac agtaccatca actaatgcaa ataaagtatc atcactacct
    12961 tttccaacat tagttcctgg atgtatttta gttcctcttt gtctaactat tatacttcca
    13021 gcagttacta attgaccatc gaatctctta acacctaatc ttttagatat agaatctcta
    13081 ccatttttag aagaacctac ccctttttta gatgctaata attgtaaatt catgtttaac
    13141 atatggagtc accccctact tttttaaaat ttttatattt tttgggtatt cccgtttaat
    13201 ttctttaacc gctaattgaa agtgatttag taataattgt gcgtctttta catgtgtatc
    13261 tttgactata cactctatat gaccttcttc tcctactact ttttcaaatt caatcttctg
    13321 taatttgtct aaagaattta taactgcaat agcattagat gtaactgcag cacatactat
    13381 atcatagcct atttcagcaa aatcagcatg tccctttaaa caaaaacctt gcaacaagaa
    13441 atcatcatta taataatatt taacttttat cataacttaa ccaataacta agcgtttatt
    13501 ttttctacaa ctatcttagt gtatgattga cgatgtcctt gttttcttct gtaatctttt
    13561 tttgctttat acttgaaaac tataactttc ttagctttac cttgttcaac aactttagct
    13621 tgaacagatg ctccttctac tactggagaa cctaatttta attctccatc tttagagcaa
    13681 gctaatactt cgtttaaagt aacaacatca cctgcgttag cttctaattt ttcaacgaat
    13741 aatacatcac cttcagaaac tttatattgc tttccaccag tctttactat agcgtacatt
    13801 aaatacacct cctcggcctc aaactcgcca gtacaaggta cttcatttga agatttaaac
    13861 ctttactgtg cggcattaca attattaatt ataaaatatt tacattttat tgtcaactat
    13921 tttatcgaaa ataacattgg ttttattgtg ctttaatgta ctatttgcgt ttaaagaaat
    13981 ctttatatta tatttttcac caattttatc aattatatcc atataatatt cttttatttg
    14041 ttcaaaaata aaatcattaa attctaccat tatgtttttg taaacagtat gttcacttat
    14101 tctcataatt tctttttcaa tactatctag tatataatgt acagatttta ttgtttgttc
    14161 accatcacaa catggacaag acattaaata atacttatca atggattctt tttctctacg
    14221 cctagcaact tctactaaac caagttttgt catacctaat acttcagctt ttcttttatc
    14281 tttattaaat tcttcttcta ggatgtgtaa aatctccttt tgatgcttct ttttatgcat
    14341 atcaataaaa tcgattatta ttatcccagc tatgtctcta attctaagct gtcttacaat
    14401 ttcttttgca gcttcaagat tagttttata aacggtttca tcaagtttac cagttccaat
    14461 aaacttaccc gtatttacat ctataaccgt aagagcttct gttttttcaa ttattaaata
    14521 acctccactc tttaaccata cttttttatt taataatttt tctattttag attctattct
    14581 gtataaatca aatacatctc tattttcctc aagacacagt ttatcaacat aagtcttatc
    14641 tatacctctt aaaatagact taagttcaga gtacttatca taactattgg taattataga
    14701 ttcaatatca tcattcacat tatcttttac atatttagtt gcaaaatcta aagacttata
    14761 taataatttc ggacctatac caagtttata ttcttttaaa atgttttcat actttaattt
    14821 caactcttct atatctttct ttatttcatc atgagtgcaa ccttgagcct cagtccttat
    14881 tattaatcct agattttcct tatttactgc cttagtaatt ttttttaatc taaatcgttc
    14941 tttttcatca gtaattttat ttgatattgt agttctatca ttggaaggta tatagacaat
    15001 ataccttcca gaaatactaa tctcagtgtt taattttgcg cctttagttc ctatttcttc
    15061 tttatttatc tgaaccaaaa tatcctgacc agattttata gtacttccct tttttaattg
    15121 taaataagca agtttcttaa atccaatatc tacaaaacaa gcttctattc ctggtataac
    15181 tttttttacg attccacgat atatattgga tacagtcttc ttatttaaat tgtcttcaac
    15241 aaaaagttct gttaatcttt catcttcaag tactgctgtt ttctgggacc ctattaggga
    15301 ctcaataact atctttttca caacttcacc tctcgagctt acataggagt cactaattcc
    15361 ccatcttcta atacatataa atctcttctc aatatatcaa catctaatgg gtcaatatca
    15421 agttcaaaca tctctaatat ctttggtata agtatattag tatttaaatt agtttttgag
    15481 cctgtagcta tagtagcagt aagagtaata tgttcatctt ctaaatttaa aacatcaaag
    15541 gttcttatca taggtcttat atctacttca accatcttac cttttttatt cttttttgtt
    15601 atgattattt ctttattatt cataaagtca attacttttc tttttacaaa ctctttcgtc
    15661 ataggtcttt tcaaatctat attaaacaaa tattctccat agtctatcac agaagaaaga
    15721 gaaggtgttt gtctatcaat ttcttttgct ttgataaaat ctatcccatc tggaagttga
    15781 attgatactt tatttaaaaa ttcctctact gataaatcat cttcttctat ttcaatatct
    15841 acatactcac cttgactttc tgcccctaaa gccaaagcat tgccataact cattttaggg
    15901 tgaggattaa atccttgaga atgtgataag tttatttcag ctcttctaaa cgctctttgt
    15961 aacaatctct gaaggtcaag atgtgatata tatatcatgt cgccttcctt tttaaatttt
    16021 actcttatta tcttactcat attagcaaag cccccttcct atatcatgta tatttattcc
    16081 acaaccattg cagttatgtc tacaatcaga agtgattttt tcttttttag cattttcgtc
    16141 ttctctaatt aagaattttt tactaatacc tacatctata tggtcccaag gaaatacctc
    16201 ttcatagttt ctatttctat gtgcataaaa atctatagaa agattattct tttccatagc
    16261 ttctttccat atatctaaat taaaatgttc tgcccatcca tcaaatttag cacctagtct
    16321 aaatgcatca tatataactt ttcctattct tctatcgcct ctagctacta ctgcttccat
    16381 caaactagtc tttgaatcat gataattgta ttttatattg ttatttctta atttatttac
    16441 taaatgtctc tgcttattaa taacttcttc tgtagtatct tgaccatgcc attggaaagg
    16501 tgtaaatggc tttggtacaa atgtagaagt actcactgta acactaaaac ttctctttag
    16561 ctttcctcca tttacttttc tataaatatc aataacttta taagccaatt tagctatacc
    16621 atctaaatct tcataagtct cagttggtaa accaatcata aaatacaact taacactatt
    16681 ccaccccatc tcaaaagctc tttctgttgc attttccaaa tcttcttctg taacaccttt
    16741 atttataaca tctctaagtc tttgagttcc tgcttctgga gcaaaagtaa gtccagattt
    16801 tcttacttgt tgtattttat cagcaatttc cattgaaaaa ttatctaatc taagggatgg
    16861 aagcgaaatc ccaatattat tagatgcata ttcatctacc aaataatcag tcaactctga
    16921 taacttacta taatcactcg tactaagtga tgataatgat atttcatcat atccagtatt
    16981 cttaactaat ttatctaata tttctttaag tcttttaaca ctcttttctc ttattggcct
    17041 gtatatcata cctgcttgac agaatctaca tcctctagta catcctctaa ataattctag
    17101 tactattcta tcatgaacag tatctatata aggtactatt agtttttctg gataatctac
    17161 agtctcaaca tcttttatta tccttttagt aggattttta gtaatccctt ctctattagg
    17221 agttacactt tgaactgtac catcttcatt gtacttgaca tcataaaaac ttggtatgta
    17281 tactccttct atgctagata ttttatataa aaaatcttct ctagtcgttt tatttttctt
    17341 ccattcttta tactcattta ctacttctaa attaacctct tcaccttcac ctaatattac
    17401 tacatctaca aaatctgcta atggttcgga attataagca caaggaccac caaccattat
    17461 aaatgggtct tccaaagttc tttcttcttt taatattggt atattggcta aatctaatat
    17521 atttaagata ttagtgtatg aaagttcata ttgaagtgta aaagtgacaa aatcaaagtt
    17581 tgttataggc tctcttgact ctaatgcaaa taatggtata cttttttctc tcattatatt
    17641 ttccatatca actgcaggag aatacactct ctcacaaaat acatcttcat ccttatttat
    17701 tacatcatat aatatatgac tccctaagtg actcatacca acttcatata aatctggaaa
    17761 gcaatgtgca tatcttatca attcactatt actagtatct ttatgaattg aatttatttc
    17821 atttccaaga tatctagcag gcttttctac tttttttaag atttttttca agtctacttt
    17881 attcatttat taaaattcct ccaaacaagc tctttatttt tattcttatt tataagtaat
    17941 attatacacg ataaaatatt ttctatcaaa aatataacta aaatattgaa tttaaaatac
    18001 atattattca caaaatagaa tagctaaaaa tatttaagaa tattttctat taaattttta
    18061 ttgaaataca atttttttac aaagtatgat atattataaa cataaaacta ttgttttaca
    18121 tgacttatca tgacatcttt tgtttcattc acctcaaaat ggggtatgtt acttatataa
    18181 tgataaattt taggagggtt aaaaatgagt aaagtttttg aagtaaaaaa agatatctac
    18241 tttactggag tagtagatga aggtttaaaa gtattcgaca taataatgga aactgagttt
    18301 gggacaactt acaactctta tttaatcaag gacgaaaaaa cggttttatt tgacactgta
    18361 aaggcaaatt ttaaagatga gtttttaagt aatttatcag aggttactga tatagctaaa
    18421 attgactatg ttgtaattca tcacacagaa ccagaccatg caggtagttt aaaatactta
    18481 ttagatataa atcctaatat agaagtttat tgtactaaag ctgctaaact atacttagat
    18541 ggtcaaataa atagaccttt taactgtcat gtaataaaag atggtgaaat tttaaatatc
    18601 ggtaaaagaa atcttagatt tataactgct ccattcttac attgggttga tacaatgttt
    18661 acttacattg aggaggacaa gactctttta acttgtgatg catttggttg tcactttgca
    18721 agtgttgatg cagaggtagt gaatagtgaa gattatctaa aatcagctaa acactactat
    18781 gattgtatag ttaagccatt tgctaaacat gttttaagtg ctgtagataa ggtggtagga
    18841 ctaaacatag aatttgatac tatattgact tctcatggtc caatgcttac taaagaccca
    18901 atggctgctg ttaaaagata tgttgagtgg tctacagaag ctgttaatac aactaatcaa
    18961 aatcaagttt ctatattcta cttatcagct tatagtaata ctttagagat ggctaaaaag
    19021 ataaaagaag gacttgataa agaaggtgca aaggctgaat tatatgattt agaagatatg
    19081 acattaacag aaatgcacga tacattagta gtgtcaaaag taatattact aggctctcca
    19141 actataaaca aaactatggt taaacctatg tgggatttat tctctgttat agacccaatg
    19201 gctaaccaag gcaagatagc aggagtattt ggttcatttg gttggagtgg tgaaggtata
    19261 actatggctg agactttact taaatctatg tcattcaaaa tgcctgtaga atctcttaag
    19321 aaaaaattct tccctagtga agagacatta aaagagtgta tggcatttgg tgcagaattt
    19381 gcaaaattag ttaaatagcc attaatcaaa cataatatta tttaaaataa atgattttat
    19441 atttaaaaat aaattaaaat ggagttgtct caaaattaat ttttctaatc taattttaag
    19501 ttcaactcct tttttatttt ccaaaatgac taatataata ataaatgtac cctttcgcac
    19561 agggtgcatt atttatagtc tgttctattt gtatactagt atacttgtat taattcacgt
    19621 atctagtata tgctactagc cctattccag aattagatgt tgtttcacca atttctactt
    19681 taataattat attatttttt tacacttttt tgataattct gctgcagaaa ggttcttacc
    19741 agtaaaatct aaagaaaatg ccttatcata ctcaaaagga taactaacag gtttgctctc
    19801 tggtgctaat gcaaccatac catatggtgt attcataaac cctattacag taactgtatg
    19861 ctttgatggg tctaaatttg aatttttgct tgtaaactta attgtattta ttgatgagtt
    19921 gattaaacta cacggaactc cctcatacat ttcaattttt ggacgtagat tatttccata
    19981 aacttcatca agtattgccc cttgttgatc tgctatcata gtgtgattcc caggtccaac
    20041 tggcgcagaa ggcttagctg gcgtaggttt cttaacagtt ggcgttggat tctgtgtctt
    20101 tactacttcg ccaacagctg gatatgcttt cttgtcaaat ggtaatgggt gagtaagact
    20161 cttgtatatg atgtctgctc ctgctgattt gttggcatca atatctacat gatacaaacg
    20221 tccatcctca aaacgaataa gattccactg atggtcattt gttgtactat gtacataata
    20281 acattcaatt cccatagcat cacataaagc tttcaagctc ctacacattc cagaacaagc
    20341 acctttttta gtaaccaatg tataagcagt attggctccc tctgctgcgt tccagttaac
    20401 atatgttgtg ttgtttttaa tgtgattatg tatagataca atccactatg gagaaatata
    20461 aaagaaaaga atataacaaa caataaaaaa taagatgatt caaattttat tttgaaacat
    20521 cttatttact ctacaaatta aaattatttg gtgaattatt taattattat tttttattaa
    20581 agaaatttta aatcttaata taaaatacgc tattatacat aataatccaa atacgaatgc
    20641 tactggaatt aacatctccg attcaaaata aaagaaatat tctaagaaaa tatctggatt
    20701 tactatattc ccattcttgt ctaaaaaatc ttttccatta tatcctaaat gctgtataaa
    20761 attacttatg ttattataat ctgcttccct ctcatatacc atattagcat tcaatccaga
    20821 agaacttaaa ttagtgtttc catctttcat atctattaca tatagtctaa gagctttgac
    20881 tttactatac ttatctttat caagtatttt ttcaagttct aacttatatt caccaatctt
    20941 tgtcatatca tgtctttctg ccaattgctt tatttctcca tcaagttctt ttcctaaatt
    21001 atacatatta tgttgtaaac tattattgta ataccacata aatccagaaa ttaaaaatgc
    21061 aattatactt atgtaaccta atactttttt aaaccccatt acaattgatt tatgcctatc
    21121 caatgataaa gatagttctt ttattatatt acataattca tactgcattt catcaccatc
    21181 atcaaatctt tcaattgctt ttttacatgc ttcttcttca tctaatcctt ctaatttaaa
    21241 ttcatttgca gattctataa gatggcatct catttcctgt tttaattcta tagtactttt
    21301 attgtcacat tttttatata gtttatctaa atattcatct attagtttca taaattaatc
    21361 cacctctccc aaaaattgat tcataacctt tttaataaat ctccattctt gtttctttat
    21421 ttctaaaaaa tctttacctt cattagttaa attataatat tttcttcttc cacctgaact
    21481 ttgttcattg ctataataag atttaattaa atttttagat tccattcttt taagtgcaag
    21541 atacattgtt ccttctttta attcaaactc acttctttct cttacttgct ttgcaatttc
    21601 atatccataa caatctttct tttctaatat agaaacaatt aatatatcga tataaccttt
    21661 taacacttct ttatttaact gcattatgta ccaccgcctt ttcacctact actctataat
    21721 gtatagtact tatatttact ataatattac atagtactct ataatacaag gtttatattc
    21781 tacaatagac ttaaaaataa taaatagctg tatattaata tttaatctat aaatactaat
    21841 atacagctat ttatcaaatt ttaaaaataa atttttgttt tattaatgtt gtcataacaa
    21901 catttacttg gttgaaatag tattacctat aagtttaaat atatataata atagacttga
    21961 ttctatttca tattattaaa atagagtaat agtttaaatt ctactactct atctataatc
    22021 tatttcctaa ttatttaatc tcaaatttag ctcttcatca tttaaatctt tcataagatt
    22081 tctacaggta ctagatttat tccaacagaa aaactagggc attaaacact tatatatctg
    22141 tttctttaga ctgtaatatt aatatttttt aaatacgttg acttttgctt tttctaactt
    22201 ctctccatga tagttctcct ctatcaagag cttttataaa tatttctgct gaagctagat
    22261 ttgtagctat tggaacatga taaacatcac aaagtcttat taacgcttgt atatctgttt
    22321 catgagcctg agatgtcaat gggtctctta aaaaaataac taaatctatt tcttgtgtta
    22381 ctattaaaga acctatttgc tggtctccac ctaaaggacc agatgctaat ctatttatat
    22441 ttaattcagt ttcatccatt attctttttc ctgtagttcc agttgcatat agtccatact
    22501 tttttaaaat tgattcataa cctatacaaa atccgaccat agtgtttttc atttggtcat
    22561 gtgctactaa tgctatattc atatatctaa tctcctcaat ttatgtttag atgtatttaa
    22621 atttagaaat ttattttctt tcttctcata cacacattta gtactaatcc taagtttgct
    22681 aaagatgtca ataacgaact tcctccataa cttacaaaag gtaatgttac tccagttact
    22741 ggtattaacg caaccgtcat acctatattt tggattatct ggtaaccaaa cattcccata
    22801 acaccaacca ctatgagggt tccataaaaa tcttttgcat ctcttgcaat tgctaaaagc
    22861 cttaatagga atatcataaa taagattatc aacactgcca ttcctattac accaagttct
    22921 tcacctacaa cagcaaatat aaaatcacta tcttgtacag gtaaaaaatc ttcttgattt
    22981 tgacttccat tgtaaagtcc tttaccagtt acgcctccag aaccaattgc aatcagagat
    23041 tgcataactt gatagtttcc ttttagagtt acatcttctg gatttagaaa agcttctatt
    23101 cttacctttt gatggtctgc catcattaaa tacatcagtg gcattgacac taatagtatg
    23161 attattcctc tttttatcaa ctttgaacta agacctgctg tgaacagcat tgcaaatatc
    23221 atacaacaaa atacaattcc tgttcccaag tctggttgtg caatcaataa tccgataaat
    23281 ggaagagaat aaactacaac aggcataact tcctttaacg tatttaattt atcctttttg
    23341 gattcaagta tctttgcata acttagtaca aatgttaatt tgactatttc agatgtctgc
    23401 aagtcaagtg gtcctaagtt aatccacgac ctagctccac cttttactgt cccaattcca
    23461 ggtaataaaa ctatggctaa caaaataaga cttattatat aaagcgcttt ataatacctg
    23521 cctagaaggt tatagtcaaa aaataatatg actataatca ttcctatacc aagtacaaaa
    23581 gctaatcctt gtttaatcaa ttgattatat gaacctgttg aattagcatg tgttgcacta
    23641 ctaagtataa caagcccaaa aataaagata gctaatactg taactataag tttccagtct
    23701 aactgtttta tcaacttaat tgtcgactta taattaatat tgattttttt caaagaatca
    23761 ccgtctttct atctttaaac atataattaa ataaaagggg catataccaa taaggatatc
    23821 ccccttttaa gcactaatta catacatcta tcttttatat tctttagtgg tatattagca
    23881 actaatgcag atacaggttt gtcatcatgc tcacctctgc atttagacat ttttatttca
    23941 agaccatcat cttctatttc tgcataatta gctattactt tcaaaatgtc tgtttttatc
    24001 atctctaaaa gttgtggtga acaatctact ctatcatgaa ctaaaactag ctttaacctc
    24061 tctttagcaa cagatttact agttttagcc tcgttagaaa aaactctaaa taaatctaac
    24121 acagatgtat cctcctttgc tacttagcca tgctaaacat ttttttaagt ctaccaaaga
    24181 atccatcttc aacttcaaga tcaagtagag gaacttcttc atttagtatc ctttttgcga
    24241 tatttttgta tgcttgacca gcaagtgact tagaatcaag tatagctggc tctcctttat
    24301 ttgttgatat aattatgctt tcatcatcag gaacaagacc taacaaatct attgctaata
    24361 tttctattat atcttgttta tccatcatgt ctccacgctt aaccatatca tttctaattc
    24421 tatttataac taatcttatc tcttttattt catttgcttc taatagacct attattctat
    24481 cagcatctct tactgctgat atttctggat tagtaactac aatagctcta tctgccccag
    24541 ctactgcatt tttaaagcct tgctcaatac ctgctggaca atctattatt atatactcga
    24601 aagattcttt tagtttctca cacaggtcaa tcatctgctc aactgagaca gcatttttat
    24661 ctctagtttg tgctgctggt aataaatata gattatcaaa tcttttgtct tttattagag
    24721 cttgcttaag cctacaagtt ccttcaacta catctactat gtcataaact attcgatttt
    24781 caagacccat tactacatct aagtttctaa gtccaatatc tgcatccaca actactgttt
    24841 ttttattttc taggcttagc gcagttccta agtttgctgc ggtagtagtt tttccaaccc
    24901 cacctttacc agatgttata actataactt cgctcataat actaccccct atttttcttt
    24961 ctatttgtct aactttgaca aatagctttc gattacaatt cttccattac ttacaaaggc
    25021 tatttctggg ctaatttctg attcactttc ataattttct tcatcatctg gagcttctgc
    25081 aatattatct gctatttgta atattttagg attaagattt ccagcaacaa catatgcaaa
    25141 ttcattgcct tctgcacctg catgtacaaa accttctatt ttacccatta caaaaacatt
    25201 acctcctgct acaaccttag caccagcatt tatatctccc ataacaactg tatttgattt
    25261 tgaaaggact tttgaaccag gtttcatatc attcaatata actacatcac cttcaaattc
    25321 aatattttca ccagatctta gggtattgac atattttgtt ggaaaattac tattttcttc
    25381 aattttttga tcttcaacaa attccacatc aaatctagaa gtaattcctt ctttaagttc
    25441 tagaatctcc atgtcagtta aacaatcact attaatttta ctaattttag ccccattaaa
    25501 aaagccaaca tatgcttcca atttgtttat tattttttcc tgtatttcct caaatggagc
    25561 ttctcttttt atatttacta ttatccctct tttattaccc ttgaactcta ctagttcttg
    25621 actacaaatt tctcttaaag acataacaac ctccagagtg atatatcatt tatattcttc
    25681 aaatacgctt taaacccttt tattttttct atataaagct aaagtttata catagattga
    25741 gctatctatt aatttgtgac tcgaaattta tattttcgtc agaagaattg ttaacatcat
    25801 tcgtcttgtt gtcattttta ctattagttg gctctaatcc catataagtt ccaataactt
    25861 ctctagttgg taaaagtgag aatacacttg aatcaccttg aggaattaca catacaacag
    25921 ctatttctgg gtcatctgct ggagcaaaag caacagtcca cgtaaaagaa ccataatctt
    25981 gtttaaatct atctatttgg tcatctgtta tttttggatt taattctttt atagcttttc
    26041 ttagatatga agaattcacc ttatcagtgt cctctaatct aacttttact ccatcttcta
    26101 attcttcctc taatttcttt ctttcttcgt ctttcaaatc tttattttct aatttctttt
    26161 ttatttcctt ttccttagct aatgaaagct ctttttcttt ttctgctttc atcttatcag
    26221 ctagtttaat cgcatcattt aggttgacat tataagatga catatgactt tttaaatatt
    26281 catactcgtt atctgtaggt attttcccac ttttctcagc tgtaccagtt tttgctgcta
    26341 catctattgg aaaatcagca aaagcactct tcgctgtacc ttgtcttgca accaatttca
    26401 taccttttgt taattctttt aagttatctg gatttttaaa aggtattttt tcgactttct
    26461 tctggtcatt aattttaact gatgaataat cacttgaaac agctctatca actacagata
    26521 attctactaa attacctcca tttgctatag cagctacata tcttgatatt tgagctggtg
    26581 tataagcatt ttcaccttga cctatagcta agttaaatgt atctgcagta ctccattttg
    26641 caaagttaaa ataactaaat actgctaaat ctgctacttc ttcaattcta tcttctttaa
    26701 catccaattt ttttagccta ttcattgttt caactctacc aggagttttc ttttctgctg
    26761 cccagcttac aatctcattg attcttttct catattcttt aggatttttt tctctagtta
    26821 tatcaacaaa atcatttgcc atttctcttt ctaaatttga ctttagaaga gcttgtgttg
    26881 atttcaattt atcttctgtg ctaggaactt tacctgctct ttcttctact tcatcttgaa
    26941 gccctgtatt ttggtcaagc ccaaataatt ttgcatactc aagtatttta tttgcattca
    27001 tatctatacc aatgcttttt ccatctggcc atgttttacc tgtaccaatt gttgccatat
    27061 atatattaca agactcttgt attgctttat ataagtttgt cataccatgg tttcctctac
    27121 catggttcca tacatagtca ccaaaagatt ttttacctaa cattataacc ccagggtcat
    27181 ttatagtaaa gtttggatcc agaccatttt ctaacgctgc cattgaagtt accatcttaa
    27241 atgaagaacc tggctgaaat tccccttgtg taaccaagtt taaaagtgga cttcctgcta
    27301 gtaagtcatt tggatttttt ggttgaagtg ctttataatc ctctgtagaa ataccttttg
    27361 caaatttatt tgggtcataa ttaggataac ttgatgaagc taatacttct ccatttttaa
    27421 catcaatagc tataagagct gctgactgtg cttttcctgc atatgcacta attggtttgt
    27481 ccccaaattt acttttaaaa gtacctcctt tagaagcaac ttcgattatc tgttttaatg
    27541 cattgtctga aacttcttgt aaatctttat ctagtgtaag atacacagta tctcctgatt
    27601 taggtttttt agattcaatt tctttactga ttctacccaa tgcatcaact ttaaccattt
    27661 tataaccatc agttcctctt aatctacttt cattactctt ttctactcct gcaagaccta
    27721 ccatatctcc agtttcataa cctttttgaa ggtatgattc tatttgagtg gatggcattt
    27781 ttcctacata tcctaacata tgagaagcta atgacccatt aggataatat cttactggtt
    27841 ctactgctat tgaaacacca acaaggtcca tagcactttc ttcaatttga gcaatcgtag
    27901 tttctcctac atccttagca atagttactg gattatattg agaatatcct tttgatttta
    27961 tcaaatccct tacgaccatt atttttcttg catcttcatc actcaaactt ttatcaattt
    28021 ctaatgcatc actatttctt actttttcaa acgcttcttt ggcactcagt ttagtttctt
    28081 ttatcttata actagccaac caatctctct tatctctttc tgctgtgaac atccatttac
    28141 taactaaaat aggtggataa tatccatttt catttagttt tgcctgtaat cttgtcgcat
    28201 ccaatctttt atcttcttga gataatattc cagctgatat aagtttgtcc accaagtaat
    28261 aaaaactttc cttagcatta taatcatttg gaataccatt ttctgattta tattccctaa
    28321 tatctctgtc atatgtataa tagtactttc cattttctac atatatagga aactcatcta
    28381 catattcttc accatttctc tccagcaaat ttataagttt taatgatatt tcatttgctc
    28441 ttgaatgctt atttgcatct ttcttattaa tatcgttacc tgaaacttga acagtaaata
    28501 agtttttatt tcctgccaac agtcttccat atctatcttt tatttctcct cttggagctt
    28561 ctattgccaa ctttttatat gttttattct cagctaactc attgtaatat tcatatttaa
    28621 atgtagtcat atagattatt tttactaaaa tagctaaaaa tatcacgccc atgactatct
    28681 taattatttt tagtctatca acttgtttat tttcttccat gaaatcacca actaatcttc
    28741 tttaagtttt aaaactgctc ttttagataa gcgatatatt actaaaccaa ttaaactatt
    28801 cactattggt gctactagta gaccttttac aaaaagtgtt gatatgttgt agctttgata
    28861 aattatacta gatgtaccta tattgacaat tgaatcaatt aaactgccta taaaaattaa
    28921 aacgaaagta ataatataac tacctttata tagctgatct ctcatgtatc caattgcata
    28981 tgcaataata aacaaaatta atgcatttac accaaaaatt ccaccaacgg ttatgtcttt
    29041 aataagccct attattgctg ctataatccc accttctaat tcatctaaat aaagagagat
    29101 tatggttaca tatataagaa ctaaattaaa gcttatgcca aaaatatcta tataatttgt
    29161 tatactattt tctatagtca taagcacaat ccccaacaga caaagtaaaa cttttttcat
    29221 tgtttttatc ccctttatcc tatatttctt ggttctatga ctacaacgtc atttatattc
    29281 ttaaaatcaa cataaggttt tacaactaca tattttaatg acttatcttt atcatcaacc
    29341 actttttcaa cttcgccaat tggtatacca tctgggaaga atccaagacc tgatgttgtt
    29401 attatatctc cttgaattac attatatgat gagtcaaaca tatatccttg caaatatcct
    29461 ttactattat aactatcttt attatctaaa gtagtatttt gagtaattgt acctttagca
    29521 ttggcatcct ttgctagttt aaagcttaca gatgccttag aatccaatag tgaaattgcc
    29581 ttactataat tgttggaaac ttcataaact atgccaacta atccactacc attcataact
    29641 atactttctt tttttacacc tgaatttttt cctgcaccta tgacaaaact ctcataccag
    29701 tttccatcat ttttaccaac aacacttgct gaaatacttg tcgccttata ctcttcttgt
    29761 acaaagttca gtgtcttttt taactcttct agcgattctg ttttatctag tttggcattt
    29821 aaagctatca tatttttttt aagtttttca ttttcttctt ctaatttttt taccttttta
    29881 gaattatctt taaaatttgc tacatcttta aaaccatttt tgataaaaga aaatccatca
    29941 tttattccct tacctacact tgtaatagca tctgaagcta ctcctaaatt tactggacta
    30001 ccactagaaa atttaccaat tgatataccc acaattccaa ttaaagtgat agcaactacg
    30061 cccgttgcta tcactttaac attaatcctt tttttctcat ttttgttctt gtcaaatctc
    30121 aaggccatca ccaacttcta attcttagta ttcatcatta atactttttc aaagatttct
    30181 tggtcttcaa cagatttacc tgttccaagt gcaacacaat caagaggtgt ttctgctatt
    30241 tgtacagaca taccagtttc ctgtgtaata agcttatcaa gacctctaag caaagcgcct
    30301 cctcctgtta gcattattcc gttttccatt atatctgagg ctaattctgg tggagttctt
    30361 tctagtgtag attttattgc atctactatt gaacttacag gttcttttaa tgcttctcta
    30421 acttctgtag agcatatttc tattgtttta ggtaatcctg agattaaatc tctacctctt
    30481 atttgcatat ttatttcttc atcatcttta aatgtagaac ctatgttaat ttttacattt
    30541 tcagcagttc tttcacctat cataagatta tattcttttt ttacataagc cactatagac
    30601 tcgtcaaatt cgtctccacc tattcttata gatttagcag taacgattcc tcctaaagat
    30661 attacagcta tttctgaagt acctccacca atatcaacta ccatattacc ttctggttct
    30721 tcaactttta atccagcccc tatagccgct gccattggct cttctataag gaatgcatct
    30781 ttagctcctg cttgtcttgc agcctcttct atagctctct tttctacttc tgtaactcca
    30841 aaaggaacac atatagctat tcttggactt acaacacctt ttttatcagc cgctttttgt
    30901 ataaaatagc taatcattga ttgagttatg tcaaaatcag ctattactcc atctttcata
    30961 ggtcttatag ctactatatt tcctggtgtt ctacctatca tttttttggc ttcttcacct
    31021 actgctaata cctctttact atcatctctt attgcgacaa ctgatggttc tctaacaaca
    31081 ataccttgtc cttttatgta aactaatgta tttgcagtcc ctaaatctat tcccatatcc
    31141 tttgtcatct tattaaaact aaaaaaacct tttttttctt tctttttttc cttagccatg
    31201 attttactcc ttcctactat taagaatcat gtttaaaaca ttcttctatt attttatcac
    31261 atataaagtc aaattatcat attttcttta aagctaaagt ataatccatc tccaataatt
    31321 atatggtcta ttatttctat tccaattatt tctccacatt taataagtct tgatgtgata
    31381 tttttatctt ctcttgatgg ttctacactt cctgaagggt gattatgcat cactattatc
    31441 ttacttgcac ttcttcttat agcttcttta aaaacctctc ttggatggac taatgacgag
    31501 cttaaagtcc ctatagatac atctacatca gatattatct cattttttgt gtttaaaaga
    31561 accgctttaa aaacctcttt ttttaaatac cttaggctat ccatataata cctctgtata
    31621 tcccaaggat tccttatttt atactttata ggtttaaaac tagctactct agagcctagt
    31681 tctaaagctg ctttaatttg ggttgatttt gataacccaa ttccatctat attacacagc
    31741 tcctctattg tcatatcttc taaatgtcta ataccctgaa tatctctatt tattatatag
    31801 ttggctaatt caatagcatt tttattcttg ttgccagttc ttaaaagtat agccaacaac
    31861 tcagcatttg ataagctttt tacgcctttg gcaagcatct tttctctggg tctttcctct
    31921 ggtgccattt cttttacctt aatggttgaa ttaaatgact ttttcagata aatcacactc
    31981 cataaaaaag atttatacta aaatattttt ttaataggtc gcctaacctt gaaattggaa
    32041 gaccaactat attaaaataa tctcctctaa tttcttcaac taacaaagct ccataacctt
    32101 gtataccata ggctcctgct ttgtcaaatg actcattagt tttcagataa tcctcaatat
    32161 cctgctctga taacgtttta aatttcacat tgctgattac atagtcaatt acctttttat
    32221 cttcgcacaa gtttattaag ctaatacctg ttatgacttg atgctctctt ccagatagtc
    32281 tttttaacat atctcttgca caagactcat cttttggttt tcctaaaata gcattgtcta
    32341 aaacaacaac tgtatcagct ccaataacaa catcttcatt atgttcagat gctacagaca
    32401 tacttttttc aaaagctaat ctcataacta aatgttttgg tgtttcacct tccaatataa
    32461 tctcatcaat ttcattttta attatatcaa atcttacatt cgtattctct agaatttctt
    32521 tccttcttgg agaggctgat gccaagatta tattcattta ttcacctcat ttgtacgtaa
    32581 tttgtaccta tttagtctat cactatattg taaaaaattc aacagaaatg tcgaaccttt
    32641 tttgttacac tattgttata tttattacca ttagaataaa tatacataaa atagaggtat
    32701 ggaaattatt ccatacctct gttttatgta ctcattagag atatggaaat tattccatat
    32761 ccttattttg tccatattac aaaattttaa tagctttctt tggacatttt tccatacata
    32821 aatggcatcc tacacattta tctgcatcta ccttatgttt ttcttttaat tctccctcta
    32881 ttgcatcaaa cttacattgt tttttgcaca ctgtacatcc aacacaaagt tcttggtcaa
    32941 tagtaacttt ctttttctta gttatgtcac ctgatattac ttttgtaggg cattttgcta
    33001 cacattgcat acaacctaca cacttattag ggtctatctt agctattttg tcttcaaata
    33061 ttattgcatc aaatttacaa gcttttacac acattccaca gccaatacat ccagcagtac
    33121 atttttcttt aacagcttta ccaaactctt tgctattaca ctctacaact acttcttgac
    33181 tttctggttt tgtgattatt atacccttag gacaaacttc tttacatttt ccacaattta
    33241 cacacttttc ttcatctatt actgctattc catcaactat agaaatagca tcaaatttac
    33301 aagcatcttt acatgttcca agacctaaac atccaaattt acacatctta gcacctgagt
    33361 ttaaaacatt tgctgctcta caatctgata ttccttcata ctcatattta tcttttgcac
    33421 ttgaacaagt tcctttacaa ataacttttg ctacttgttt ctcacctgct tcagcactta
    33481 ttcccattat ttctccaact tttgcaccaa catctgctcc acctacaggg caaccattaa
    33541 ctggtgcatt gccttcaact atcgcatttg caagacctcc acaacctgga aatccacaac
    33601 ctccacagtt ggctcctgga agtactccta gtatagcttc cactctctcg tctacttcta
    33661 cagcaaactt tttagatgca aaatctagta ctatcccgaa gattaagccc ataattccta
    33721 aaactaatac agctgtaagt atcaccattt tcacccctct tttttatagc tttataagtc
    33781 ctgtaaatcc taagaaagct attgacatta aacctgctga tataagcgtt atagggaaac
    33841 ctttaaatgc ttctggtata tcagctaatt ctagtctttc tcttatacct gcgaatatta
    33901 ctattgctaa agtaaatcca gctccggctc caaatccgtt tattattgtt tctactaaat
    33961 tataactttt ttgaacatta actagagcta taccaagaac tgcacagttt gtagttataa
    34021 gaggtaaata aactcctaat gcttgatata aagatggact catcttttga attaccattt
    34081 caacgaactg aactatagaa gctataacca atataaaagc tattgtttgt aagaatccag
    34141 ttccagtttt tactaataaa atctgtataa aataagttat aattgaagct agagttaaaa
    34201 cgaacgtaac tgcaactccc atccctactg ctgtatcaac cttcttagaa actcccaaga
    34261 aaggacatat acccaagaat tgagatgtta taacgttatt aacaagaacg atacttaaaa
    34321 ataaaagtat taaattcatt tattacacct ccactatgct tttttacttc ttaatttatt
    34381 aaatcctgca aataagaatc ctaatgttaa gaatgcacct ggtggtaata taaatattaa
    34441 tactggttgg aaagatgctc caaataatgt cattccaaat aaactaccat ttccaagtaa
    34501 ttctcttact gcacctatta ttgtaagtgc aacagtaaat ccaagacctt gacctacacc
    34561 atcaactatt gaaggcatag ctcctgtttt gaaagcaaaa ctttctgcac gagctaatat
    34621 taaacagtta acaactatta atggtatata tataccaaga gccttatcaa gagcaggaac
    34681 atatgctttt aaaagcatac ctactattgt aacaaatgta gctataacta ctataaatat
    34741 aggtattctt atcttatctg gtgtaatttt tcttattaaa gatataacta aatttgcaca
    34801 tattaaaact gctgctgttg aaagacccat accaattcca tttattgctg aagttgtaac
    34861 ggctagtgtt ggacacatac ctataacttg aacaaatgtt gggttctcat ctattagccc
    34921 atttttaaat actttagcta aattcataat taaccctcct tatttattaa gagcactatt
    34981 gaaaacatct atagcagcat ttactcctgt agtaactgct gtagatgtaa tagttgcacc
    35041 tgatattgct aatatttgat tatctgctgt tgtacttcct tttgctactt caagaggctc
    35101 agcagttttt cctttgtatt gacccttaaa tgcatcatcc ttagcttttg ctccaagacc
    35161 tgctgtttct gacatattac caatgtcaac acctgtaact tgaccttcag atgatatacc
    35221 taccataact tcaatttctc caccatatcc acttggttta gcttttaaag tatatccaac
    35281 cacttcagaa ccatctaaac cttcatacac ttcagctata agaccatctc caaggccttt
    35341 gtaagtatct tccttcattt ctttaaattc ttttgcttct ggaaggactg cttttctaag
    35401 ttcattattt gcttgtatat ttctttgttc tattactgga gctgtaacct gattagtagc
    35461 tccaagagct aatgcagcta ttgcacttat agcaagtaaa gtaccaccta atctcaccat
    35521 actgttcatt atttagccac ctccccaaat actctatttc taacaaactt atctataagt
    35581 ggagcaagac agttcactaa tagtattgag taagatacac cttctggata tccaccatac
    35641 attcttataa cagttgttat aagaccagct aatacagcgt atattatttg acctttttta
    35701 gtcataggcg aagttgtata atccgtaagc atgaagaatc cacctaacat aagtcctcca
    35761 gcaaacagtt gatatattgc gaaagttata ttaaatccac ctaaaaggaa tgttaatata
    35821 aatacagttg ctatgtaaaa tactggtata acataattta ttatgccttt atacattagg
    35881 tatactccac ctaataataa taatattgct gatgtctcac ctatacatcc accaatattc
    35941 cctatcatca tgtcagcaag actaattcca ttgctagaaa ggtctgccag tcctgctgct
    36001 ccatttttca aaaagcttaa tggagttgct gtagtaactg catctaaatt tttacctatc
    36061 cagttaacac ctggttgagt ccaagcagtc attgctactg ggaatgatgc taataaaaat
    36121 gctcttgcag caagtgctgg attgacaaag ttacatccaa ttccaccaaa taccatttta
    36181 actactaata ttgcaaatat tcctcctact agacacatcc accaaggaag tgatgctggt
    36241 acgttaaatg ctaataataa tcctgttaca acagctgaaa aatctcctat agtacttttt
    36301 tgcttcgtaa atttttgata taaaaattca gtacctactg taccaagtat acaaaaaaac
    36361 atagcactca aagcattaag tctaaagaag tatactcctg ctactgctgc tggaaggagt
    36421 gctataataa cttgtttcat tatatatgaa gtatcttcat tacttctcac atgaggagaa
    36481 gatgatacta tcaacttatt ttccatcgtt tttccccctt aactttcttt atttctgagc
    36541 agcttgcttt cttctctttg ctcctatttc tctctttgct gatcttatag attgaagtaa
    36601 tggtcttctt gctggacaaa taaatgagca agacccacat tctatacaat ccattgctct
    36661 atgatattca gctgtgtcat agtcatcttt taaagaatat gcacttatga aaagtggttg
    36721 taagaatgat ggacatacat ctgtacatct tccacatctt aaacagtttt gaatatctgg
    36781 agtacgtgat tcttcctcat ccaaacataa aattcctgaa gaacctttgt tagtcgctat
    36841 ttcagtagta tattgagcta ttcccatcat aggtccaccc attataacct taccaatctt
    36901 cttatcttct ttaaatccac cacattgttc tattatttct gaaactaatg ttccaacttt
    36961 tgttattagg ttctttggct ctttgatgca actacctgtt attgtagtga ttctttcaac
    37021 caaaggcata cctgttttaa tagacttagc tatctgtgct gctgtggcaa cgttgtcaac
    37081 aactgcacct gctgctattg gtaatccacc agatggaact tctttgcctg tacacgcata
    37141 aataagttgt ttctctgctc cttgtggata tttcacttga aggccaacaa cttcgatttc
    37201 gctatagtct ttagctacat tttgaattgc ttctatagca tctggtttat tcgtttcaat
    37261 tcctataaat ccttttttga catctaatac cttcattaat gctcttaaac caaacacaac
    37321 atcttctgga ttttcaacca ttaatctgtg gtctgcagtt aagtatggtt cacattctgc
    37381 accatttaat ataactactt ctgccttgct atctggtgga ggagatactt ttacatggtt
    37441 agggaatgta gctccaccca tacctactat tcctgcctct tttatgatac ctactatatc
    37501 ttctttgctc aaatcctcta actgaccttt tggttgaaca ctctcatgta attcttcctt
    37561 aaagtcattt tcgataacta cacattgagc tgaacctcct ggtacttcat gttgttcaat
    37621 agcaataact ttacctgata ctgaagaatg aacgttacat gaaacaaaac cttgttgttc
    37681 acctattttt tgcccaaact ttacttcatc accaacttca acaataggtt tagctggtgc
    37741 ccctatatgt tgctgaagag gaatgtaaac tatctttgga gcttgagctt tttcaagtgc
    37801 ctttgtgtta gagtactctt ttctatatgg aggatgtatc cctcccttaa aagttaagag
    37861 tttcattgtt ttaccccctt atctataaaa aaacattttt tctgccttct ataagtatac
    37921 tatatatatt aaaaaatctt aacaattgtt aaaaaaatat ccgtattttt ataatttatt
    37981 ttataaatca acacctctat attaagtcaa attgaggaaa aggattgctt aattaaacaa
    38041 tccttttcat atagctttct aaagtccaaa ttttctgtat tttattttat agaatttata
    38101 aacatagagt actcctgcat ggatgataaa agcagacttt tacttacact atatttttct
    38161 tcatttgctg cttttgaagc tgtatcaatt ttttcattca cattctttac atactctatt
    38221 aaattatctt taaataaacg catttcagaa taatcaatct tttcagcttc ttttgatata
    38281 ttttgtgata ttgcctttct atttgtcaaa atagtattat atttttccat atctacatta
    38341 ttttcagcat tcgaccaaaa acttgattct tctttaaagt ttgttattaa tttatttaat
    38401 tctttagata tactctctat ataagcgtag ttacttgtat actctaaaga caacataggt
    38461 gcatctaagt gtgataaaaa tgcatctgga aacacctttc ttacactgct tatctgcttt
    38521 ctaacatcat tttcatcaaa agaggaatag gtctgtattt tttttactcc atccttctcc
    38581 ataactgaaa aaggtatgtc attattaact aatgatgtct gtattttttt caaatcatca
    38641 ttattatcta ttgcagcaac ttgaatagta tacatatccc agccttctat agtagctaat
    38701 ttgacttctt ctggctcttt agtgttattt accgcttttt ctggattagt ttcttgttta
    38761 tcatctgaat tggtttgtga tttagatttt ttcccttttt ttattgaatt taattcatca
    38821 gatatatctt tataagttat atctgatgaa ttatttagaa atggtatttt agccgataca
    38881 tactccaata tcttgctatc ttttattttt gtataagaat aatagccaat tagacacaag
    38941 caagcaatcg ttataacata tttacgtaaa tcaatcttat gtctattatt attaaatcct
    39001 ctgtatatcg tttttcttct attcattagt ttgtccctcc caaaattata ttattaataa
    39061 agtatatttt taagagtcac aatttatgaa tataaataat tatttcttct aattttttaa
    39121 taagaaattg tacttactaa acaaatcttc ttgcaccaag taatacttta gaccaatatg
    39181 aactagttaa attatccact ttaaccttat taggattagt ctgagtacca ctacaatgga
    39241 taaatttact atttcctacg tatataccta catgattagt tgttccatca ccatcagtgt
    39301 caaaataaac taaatctcct ggtgcataat ttcccataga aattgcactt ccaaaattag
    39361 cctgttctct agaaactcta ggaatagaaa cgcctacaga atgtttaaaa acatactgtg
    39421 taaatccaga acagtcaaat ttatcaggtc ctgaagctcc ccattgatat ggtattccaa
    39481 taagagtata tgcaaaatct acgattgttg aaccttttgc tgtagattta tctgaatttc
    39541 cagtaaatga tggctttgag ttactattag attgagaatt ttggttagat gatgaatttg
    39601 gtttttcttt tgtatcctca ttagtctcag aaatataact ggcactcacc catccaattg
    39661 ttccactcga taactttacc ttataccaac cattactttt agcttttaat tcaacaacat
    39721 ctccaccatt taattttcct attatagaat agcttgtccc aggaccactt cttacattta
    39781 aactcacagc agatgttacc ttaccattct tgccttcaat tgattcttcg ctagatggtt
    39841 ttttatcaga attattagta ctatttgaat tactagaatt gttcgttcca acatcttctg
    39901 atgttttaga aatatattgg ctactagccc atccagtaac tccatttgat agttttattt
    39961 tataccatcc attgctttgt tctagtaact ttactacatc tccattattt gctttaccaa
    40021 ctaatgaata gttagtacct gcaccacttc ttacattcag tctagaactc acatttactt
    40081 taccatttcc actaatagtt gtcccacttg aagttggatt attttgactt gatgtacctt
    40141 cagacgaacc acttgttgat atgtattttg cacttcccca tccaacaaca gaactcgata
    40201 cccttacttt ataccatcca ttagactttt ctaaaatctc tacagttttt cccttgtata
    40261 attttgctac cttatcgcaa tcagaacttg gtccacttct tatattcaag gcacttgctg
    40321 ttactgttcc cttttcaaga gcacttgcat ttatggagct aacagatact gctactgccc
    40381 caattcctaa agctgctata gcttttttca ctactatcac tccccccaac aaatttttat
    40441 agtcataaat tcataacatt attatccatt tttagtccct acaatttaat gataacttat
    40501 tttcttatta attgtaaaga aattataaac atatccttaa aattagtgat ttttaggaag
    40561 tatttcaatc caataattat ctgggtccgc tataaaataa attcccatac tttcattttc
    40621 aaaacatatg caatctaaat ctttatgaag tttttttgcc tcatcaaact cctcaacttc
    40681 taaagccaga tgaaattcat tgtctcctaa attatatggt ctatcccaat ctctaagcca
    40741 agttaattct aatgtatgac tagtaatgcc atctcctaaa taaactaata taaaacttcc
    40801 atcttctgct tccttgcgct ttacctcttt aagccctaaa gcttttttat aaaaatctaa
    40861 acttttttct aaattagtaa cattgaaatt gttatgacaa aaattaaact tcattttgaa
    40921 atcactctcc aatttttatt ttttttatat tatatcatta ttataaattt atttaaatca
    40981 tattaagtta aaaatttgaa tgatgatgac caattaccaa atcatggtaa attatttaaa
    41041 agaatatgtc aaaaaaagaa taagctcttt caaatgcata aaaaatttat acccaagtta
    41101 tacttaatca tgcaaattgg cagaacttat tctttaaaac tctaactttt aagtttttct
    41161 ttaataatat aatttattac tttttttcta atatcttcag cattataatc atcttctact
    41221 tctatatcca aaagttttgc tattgacctc atatcttctc tagtctttga cctatagctt
    41281 gatattaatt catcttttat tttctctggt tctaaaagat attcttcatc tcctcttttg
    41341 aaaacaacgt atttttgagc atatgaactt ctattgtagt atatatatct tgatattttt
    41401 cttaaaattt gattatatga aagtcgcttg ttaatatcaa gttttaagta ctctgaaaaa
    41461 gcaaatagct ctttttttga tttaaataat gcttctttct ttatagttct tgatattgaa
    41521 gttacacttt cttgatatgc acactttttt aacattttta aatatactct attaagacca
    41581 atactttgat tgaatttttt tatattatta ttaataatct tatccaagtt accttcaaag
    41641 tatttttttg ctcctttttg tgatacataa ctctcaagtt ctttgtgggc catatcaagt
    41701 atcatttttt ttaaatcctc tttatatccc ctagattcca tgatactccc tcccttagct
    41761 atttatagta tattcaaaaa gctcaagaaa tgtgagtcat tacaaaattt ttttactttt
    41821 tatgtatata ttaatttaaa aggggagata atagtaatgt aaaatcaaac atactcaatc
    41881 tcccccattt ttagacctct attactcccc caatagaggt ctttttttag tttatcaatt
    41941 tctattcaat tccctttagt tcaaatccta attctgctat cacttctttt aatttttcaa
    42001 tagatacatc atctttcata tctacagatg cacacttatt ttctaaatct acatctaaaa
    42061 cttcaatacc atctatatct tctgttaatg cagttttaag atggtttaca caatgaccac
    42121 aactcatacc ttctactaat agtttcttct tcatttaatt acctccttat accctataag
    42181 ggtattctat catatagttt atacttttta aatagaaatt aatcatttaa tttgattcat
    42241 tcgattttat atctaaatta gatattccct catatacatc aaaatcattt ggagtaattt
    42301 taccttttaa tctactatta cttacaatat agttgtttct cacagctaaa cttataaatg
    42361 ttgcattatc ttttatatat gtctgtacat ctttgtatgc tttttttgtt tcaatttcag
    42421 acgtagaact tgtaagtgca gacatatatc catttaactt atcatcagta tatccactac
    42481 tcgctataat actacttgca tctggtataa ttgatagttt ccatccaact aatgctaaat
    42541 cataattttt agaattaaga gcattttcca tatccttatc tgagagctga tttactgtac
    42601 tatttattcc tatagctttt aagttttcat ttattatgtt tgcagttttt attctctcac
    42661 tattatctct gtttactatt atctttaagt tcatttcaga aagacttttc tttacttcct
    42721 ctttttctct attttttttc tcttcagctt cttttttgat tctatcttct tctgcttttt
    42781 taacttttgc ttcttcttct ggcgtaagct tctttgagtt ctttttagct gattttttct
    42841 gattttttac tttattgtca ttttgattta ctgagtttac atttgcatat tctaccttag
    42901 caagacactc tatcgcctta tctttattaa actctaaatc cttcatttca ctattatagt
    42961 attttgattt tgaattaagt ggaaaattaa ctggagtagc atcatccata taaccttcac
    43021 taataattct atctttatct atagagtgtg caattgcttt tctaaaatta acatctttga
    43081 aaaaaggatt attataatta aataatacac actcataatc tcttccttga tattttgtta
    43141 tattaaactc tttttcttga aacttactta aatcatttaa tgtaacattg gttatatcac
    43201 tatcaagagc cataaccata gatgtccttg catcttcatt aggcaccata cctactttta
    43261 tgttcttcat ggtttttgga acttcttcat agtaatcttt gtttacagat aaaaccattc
    43321 cttctctttc tgtatatgat tctattttat atttcccatt tcctatcata ttattattct
    43381 tgtcatttac atcttttgaa tctaaatgat tttgagatac aattggaaaa attaaactat
    43441 caattgaaaa agcatatcta gctttaaatt ttattgtaaa atctttatct gatttaatat
    43501 tcacagaagc tatattttta gtaaattcat tatatggact atctgcactt ttttgaatta
    43561 aaccaatagt atagtttaca tcatttgaag taacattttt tccatcatgc cactttgcat
    43621 cttttagctt tatatctata ctcattccat cctgagctat attatactcc ttaactaatt
    43681 gaggaattac attgtagttc tcatctatag taaataaact atcataaact aaattcatta
    43741 tataaccaac agacttatca gtattagtta taggatttat agtttttgga gttaccattg
    43801 ttaagtttat atacttagca tctatagaac tttgactttg taaacttgaa tcactactac
    43861 tttgttttgt tttagtatta ctacaaccag ctaccataag agttatagct aaaacgactg
    43921 ttaagacttt tattctcttc attagttttt tcctttcttg ccatatatct tattataatg
    43981 ttcgtaatta ttttttactt tttctacata ttttgaagtt tgcttaaaag gtatatcatg
    44041 tagattttcg ccatctttac tatattcatt attttctaac cattttttca cattacctga
    44101 accaccatta tatgcagcta tcactaaatc taatttacca aattctttgt ataacttact
    44161 taaataccaa cagccaagtc ttatattagt ctctgggtcg aaaatatcca cattttttaa
    44221 atttagttcc tctgctcccc aatctctagt tatgtctaat atttgcatta atccttttgc
    44281 ttcctttttt gaaacagcag aactattaaa cttactttcg gctttaataa cactgtaaac
    44341 tatattttca tctaaattaa attctttcga atacttttct acatactctg aatatttttt
    44401 aggatataaa aatttatgta ttactttgct ttccattaat agtgccccaa ataagattat
    44461 aaaaatagat agaatcaata ctttcttact attcacgcca attctccttc atgtactcaa
    44521 taaatttatg tgctttactt tctaattcag ttattgttcc cgaattatct attatataat
    44581 caccatattt ggatttttct tcctgactca tctgtgaatt tatcctacta agtgcttcct
    44641 gctcactgca attatctctc ttttgtattc tactaatttg tacttcttgt ttacaagtaa
    44701 ctactaatag cttgtcaacc atatctaaaa aaccactttc taccaatatt gccgcatcta
    44761 acactactat attttcgcct ttttttctca atttttctat ttcacttatt attttttctc
    44821 ttattcgtgg atgagtcaag ttatttaatt ccttaagttt ttcttcatca ctaaaaacta
    44881 ttttcccaag agcttttcta tttagagttc catcatcatt ttttatactt tgcccaaaat
    44941 ggacaaatac tttttccaat aacaacttat cctcaaatat cttcctggat ataatatcag
    45001 catccactat aggaatgtta agatttctaa atatatttga taggctgctt tttccacagc
    45061 ctattcctcc tgtaagtcct aatatcaaca taaaatcacc tactttgtct cataccaaga
    45121 actacctaca tttaaatcta catctaagtg aacatccata tttacagcat tttccatttc
    45181 ttcttttact attttcttaa ctatatctat ttcatcatca attgcttcaa ctatcaactc
    45241 gtcgtgaact tgtagtataa gttttgattt caaattgttc tcttccaatt ttttatgaac
    45301 atttatcata gcaattttta ttatatctgc tgcacttcct tgtattggtg cattcattgc
    45361 aaatctcttt cctctatttc tttccataaa attgcttgac tttatttctg gtatatatct
    45421 tcttctgttt aaaattgtta cagcatatcc atcctcacta gccttttcta ctgttgtgtc
    45481 cataaactct tttattttat ggtatttagc aaaataactt tcgatatatt ctttcgcctt
    45541 tggaacaggt atatttaaat catcagctag accaaagtca cttttaccat atataatacc
    45601 aaagtttaca gctttagctg cacctctcaa ttcactagta acatcttcca taggaacatt
    45661 aaaaacttgt gaagctgttt tggtatgaat atcttcatga tgtttaaatg cttctatcat
    45721 attttcatcc tgactcatat gtgcaagaac tcttagctct acttgtgagt aatcggcatc
    45781 aaccaactta cagttatcat ctgcaataaa cacttttctt atttttctac ccatttccat
    45841 ttttataggt atattttgaa gatttggttc tgtagatgaa attcttcctg tagtcgttat
    45901 agtttgatta aatgatgaat gtattctatt actttctgga tttattatat ttaaaagtcc
    45961 ttcaacgtaa gtagaattta actttactat ttgtctatac tcagttatct tatctattat
    46021 tggatgctta tctcttaatt tttctaaaac atctgcatta gttgaatatc cagttttagt
    46081 ttttttaatt attggaagct ctaatttttc aaataatata acacccaatt gttttggtga
    46141 atttatattg aatggctctc ctgataattc ataaatttcc ttttctaatg tagctattat
    46201 ttctttaaat tcaacactca attcatccaa tatacttttg tctactttta taccttcata
    46261 ctccatatga cctaagactt ctactaaagg catctctaca tgataaaaaa gaccatccat
    46321 actcatatcc attagacttc tttccatcat aggaactgtt tctttaactg tgcatattat
    46381 ttgacccata tactctgcta aatcctcaaa ttctaaatcc tcatagtttt tagatttaac
    46441 accctttcct aataattctt cttttgattt aacctttttt gataaatact tcatagctat
    46501 tgcactacaa tcatatgagg tagatgaagt tgaatctatt aagtactcag ctattgttat
    46561 atcaaaatac atattttcta agtttatatt atatggtttt aaagatatat aatcatcttt
    46621 taaattatat ccatactttt taatttcatt atttgaaaat atactatcta actttgacac
    46681 ttcatttgaa tctacataat atattttttc tccatctaca cttataaaaa tatatattat
    46741 atttttttca agtatattac cttgtcctct aacagttttt aaaataactt gtcctttttt
    46801 gtttacttca tctataaatt tttctatatc ttttaatttt aatatttcta ttttttcttc
    46861 tacgtgttct gtgtcatctg aatcgaccaa atcaactaat cttgataaga gacttgtaaa
    46921 tccaaagtat ctaaatttat ctaaaagttc atctttatca taatcgccaa aaatcatact
    46981 atctaaatcc acttctattg gcacatctct tataattgtt gcaagtcttt tactttgaat
    47041 agctatttct ttgttttcct ctattttctt ttttacacta cctttaagct tatctgtatt
    47101 ctctattaga ttctctatac tagaaaactc ttttataagc tttatacctg ttttttcacc
    47161 tattccaggt actccaggta tattatcaga cttgtctccc ataagaccct ttaaatcgat
    47221 aaattgtgtt ggagtcatct cgtatttttc aattacagaa ttaaagttgt attcttcaac
    47281 ttctccaact cctttttttg ttataagagt agttgtattg tctgaagcaa gttgaatagc
    47341 atctttgtct ccagttacta tgtatacctt atatccattt tcttctgctt ttttagaaac
    47401 agtacctata atatcatctg cctcatagcc atctatttcc aatctattta ttctaaattt
    47461 atctaaaagg tcttttaatg gctgtaattg ttctgccagt tcatctggca ttccctttct
    47521 tccagcctta tactctttat attctaaatg tctaaatgta ggtgcttttc tgtcaaatgc
    47581 tacacttata tgtgttggtt tatatatatc tatcatttta aatagcattg ttgtaaatcc
    47641 atatattgca ttggtcttta gcccttcttt attattcatt tctggaagag cataaaaagc
    47701 tctatttatt attgaatttc catctattat aattaatttt ttttccaaat tatatcactc
    47761 ctaaaaatta tctatttttg taacatatat attatatacc aatatactgt tatttaatat
    47821 tcccataata ataatagtat attaaaatta aatatgttgc aaacaatata tttttttgct
    47881 ataataatat acatgacatg cccgagtggt ggaattggta gacgcagtgg actcaaaatc
    47941 caccggacta atactccgtg ccggttcgag tccggcctcg ggcaccatta aaaactccta
    48001 aagctattta ttcaatagat taggagtttt tttatttttg atttaaattt taagttattt
    48061 tttgaaatta ctgtgtcttt ttaaaattta tataattata taaatttatc ttctagcatt
    48121 aatttaaaaa ctaatttatt tttatataat tttctatttt atatcataac ttattacaac
    48181 tttttatcta atttattgta tacaacaaca ctacaaataa attgaaataa aataagcaat
    48241 ccaagaacaa ctaaaaccca cttgcttatt accattacag aaaatacact caatatagaa
    48301 acaataaata taagaattac aagaacatag gacatgattt gattagtgat acgtccagct
    48361 ttatcacgta acataattcg tctttcatca ttcatctcta tcctttgatt ttttatcttt
    48421 tcttcataat ctactgctct ttcaggttta ctccaataca tatattgaag aatcatacat
    48481 acacctggac ctaaagcagc tccagataat ccccaaataa aagcctcaaa tgaaaactca
    48541 gtaaatgtag ccaataaaac aagtaaaatt ccacaaataa aatatataat tccaacatat
    48601 aacatattct ttttcatttt tactcctcct cataaataaa tatttcttca atactcatat
    48661 caaagtatct agctatctta aaggctaaga tgatagaagg attataccta ccattttcta
    48721 atgaacctat agtttgtctt gatacttgca aaatagaagc aagttcttct tgttttatgc
    48781 ctctttcttt tcttatttct tctaatctat ttttcacatc tatccccctt taatatctca
    48841 actacggaaa gtctactttc cataattata caactatatt ccatttatgt aaagcttact
    48901 ttccatattt acaaaaaaat aacagactat tttcagcctg taattttaga atttcatgtt
    48961 gtttattaat tttaatgtta tttattaatt ccatattatt tattaatttt aacttggttt
    49021 ctgaattgga atccaaactt ctgtagtata atcaggagca ttgatatcgc cattgggata
    49081 aagttcaata tctggtaaat tagcatacac atacccagaa cttggtaacc actcagtaat
    49141 aattcttttt tgcaattcct gtattgccag agcatttggc attggagaaa cacattcaaa
    49201 tactgcccat gtgcctgatg gtattaagta ttcatgagta tcttctaata caggcttatc
    49261 tgttggagca gcaatataat aatctaaacc cttactattt gtacacacac taactccaag
    49321 caatccacaa ggttcattat ttattaactc tgaaagttta tcaattgttc cattttttat
    49381 gcatttagcc cacaacttag gaactttctc aaagcactct tctacaatca tgttcatact
    49441 ttcttttata ccaacaatac gaaacccttt cttttctaca atacgatatt gcatttctat
    49501 atttcctttt acataaatgc ttaatgtcat cttaggatat gcttttaaaa caacaccctt
    49561 tgctctagct gcagatggag aaatgttatg tatattttga aaagctctat taaaagatgt
    49621 tggtgaatcg taaccatact tctcagatag ttctaaaatt ttgatgttac tgttctgaag
    49681 ttcaaatgca gccttagtca tacgtcttcg tctaatatat tcagataatg gtacttctgc
    49741 aatatatgaa aacattcttt gaaaatgaaa ggttgaacaa catgcaattt ttgctgcttc
    49801 tgcataatcc actccacttt cgagattttc ttcaatataa gtaatagctt gatttaattt
    49861 atttatccat tccatttgat attacctcca agcaaattat acagtttaat aaaataatat
    49921 acctctctat tcatgcacaa aattgtaagc atttaaattt ataagcattt ttcaatcaat
    49981 acaataaata taaaaattgt attatacaat aaaaaagttc aataacatta tattataaaa
    50041 tgttattgaa ctttttataa aaattaattt aagtgtagtt aaattaagta ttacactatc
    50101 ttatatatta caatatccta ctatcttata gtcttctggc actttataat ctttgtctgg
    50161 agtacctaaa ttccatttta aatcaaacat agttgcactt attattgttg caaaataaag
    50221 cataactata ccaatgtcta ttttttcttc atataaatta gtatgaccat cttttctaac
    50281 agctaaaact acagtgcctc catcaactat aaatctccat ggttgtctat ttaatgtaga
    50341 tggagctaat cttgcatagt gaaatgcatc aagaagtgct ctttcttcta agatttctat
    50401 acttgcaaca tttccccatt cgttcatata aacaacatct tctacaccta gtctagtaga
    50461 ccccttatct gttacaactt gcatatcaga ttttgaataa ttttgcccag tttttgtagc
    50521 atttaatact ttagcattac ttacattttc tccatatcca atagctataa ttcctgttac
    50581 ctctttatct gaattaattc caagtttttc ttttaccttt tttccatctt caaaagtaac
    50641 ccaacaagaa ccaattccaa gttctgttac tttaaagatt atactttctc ctatgtaccc
    50701 tgaattttca atgtaattat cttttatgtc agataatatt ataatataac ttggagaatc
    50761 tatcatattt cctttgtacc ctgctacttt atctaaatta ttaaaaactt cacttctatt
    50821 cataattctt acatcaacat ctatttctgg aactagtttc ttacaagatt ctgagtattt
    50881 cttaatttca ctaagatatt cttctttaat atctgtgttc ttatattctc taacagactt
    50941 tctatttaat atgattgttt tataatccat aattttatcc tccccaaact agtattgtct
    51001 taattatata cccattaata tattatttta ctcatttatt taataaataa agattacaat
    51061 tttgtaatta ttatttatta ataatattcc acttttacat taataatgac cagttgtgca
    51121 aaatccattg ccaaccacca aataaaaaca tattataaag gtaatataaa ataacaaaca
    51181 aatcttaaca aaaaattaaa ctttttttac acgaaagaag gcttcccgtt gccttcttta
    51241 tttttataaa aaaataagct gtttctaaaa tgatttcaca tttttgaaac agccaagtta
    51301 ttctataatg acaactataa tttgaggatg tgagggagag ttgtcattat aaatttaggg
    51361 taattatatt atgttttaag ctagttcttt ctatatttat ataatacatt ttatatatga
    51421 attttatatg agattggtat aaatgatata tgaaattaca tatgtttata ttatattaaa
    51481 ttttattatt acattttcca caacaatact catatgtctc cacattattt ggaatattta
    51541 caaatacaaa ttctttacag ttctcacaat actttaatat agatatagtt ccacaagtgt
    51601 aacattcaaa tattacacaa tcctcatttt cctctactat ttcaaacttt ttactatgtt
    51661 caccacactc tgggcacttg tgattattta cattcataaa tactcctcct tattagtata
    51721 gttacatgtc aaaaacttaa ttaatgtatt ctatattact tattatatat tctataataa
    51781 atcatattct aatataataa aacaaattaa ataaaaaaaa accataaatt ttaaatttat
    51841 ggattaattc ttgtaatgat aactctattt gaggatgtga gggagggtta tcattacaat
    51901 agggtattat attatggttt ttaagctagt ctttctacac ctcatatgat acatatgcta
    51961 tatgaatttt atatgatttt tacataaata ttttatgaaa tttaaatttt ttagtcattt
    52021 tgaaatttgt aaaaagttct ctaaaaatcc acagcatcat aaacagccaa aatttaagta
    52081 gaacatcata aaaaaatttg tccaataatt ttaacaagta atctcacctc attataatta
    52141 ttgacacaaa cttaaataaa caatctataa atcttctata aatcttaaac tttcccccta
    52201 ttgatacaca atttctagtt tgtcaatttt attttgcatt tctaagaaaa tttcttcaac
    52261 agaattgtta aatatattgc ctaaatagat tcttttatct ttttgaaatg aataagattt
    52321 tacactacca tcagaagaaa tatataaatg actccactct gctccatatt ttgaaaacct
    52381 ctcctttgta tctttaaacc tcatatcttc tatttttatt gctttttctc tgtctcctct
    52441 tactacagtg tgaccccttt ttattaaact ggttattttt atatcttctt ttaatttaat
    52501 acgtgtatca tcattttttg aatatacttt ccatatcact ttatgacgat aattatctaa
    52561 ttcctttatt aagcttctat caatagtaaa actatcttta gttaccacaa ttaaatcaaa
    52621 atggtctaac atatattgaa atatttctct catactttta ttttttaaaa tatcctcacc
    52681 aataacctct ataaatctac agcctttctc atacatgcag ttcaataaac ttgtgatttc
    52741 agaaaaataa tcatttgtat tattatctcc atctatttct atagtaaagt acaaaggagt
    52801 aatgtatgat ttatttcctg ttatctcaaa tacacttgat agtgtctgta tatcatcttc
    52861 aaacgttaag tatacattct cataaaacat aaaaaattcc ataacttttt ctttcatatt
    52921 atggatattt ctatatctat tggataatat atttattata tcgtcagtag tatttttccc
    52981 atcacattca gccagtataa caatttgctt ccaacttaag ctatccacat ttccgatact
    53041 ttttttatat ccatcttctt catcataaat aggaccaatt ataatgtttt cttttggcca
    53101 cagatgaacc ctagttccat cttttaattt cggatattta ttttttacag actcataaaa
    53161 tctacttgta tcacccatga ttctttacta ccccccatag tttaaatatt ctatgctaaa
    53221 aatagcactt tatttttatt aattcaacaa ttattcaaaa ttggtcatta tatatgttta
    53281 aatggattta acatcaacct tatttatatg ttattgatgt ttatatatgt tatataaaat
    53341 cttatttctc atacttcaat gtaatcttaa ctatctctgg tttacaatta agcctaatta
    53401 aaacttttga gcctcctaag cctttattta aaatcatagt gctattatct ttttcatatt
    53461 ttccccagtc atacttagga aagaactctc tatttggaga taatacacct ccaacaaaag
    53521 gtattcttat tatacctcca tgaacatgtc ctgctaaaac caaatctgct ccccattttg
    53581 catatttttc aaagaaaaat ggtgtatgtg ctaaaagaat gttatattca ttttcattaa
    53641 ctttacctag attgtcttct acaaaattgt cacttagttc taattttttg ctattagcaa
    53701 ataaatatgg atagcattcc aatggtataa ttaaaccata taaattaaga tggctattac
    53761 ctctatatat tcttacagat tcattttcca tatttattat tttctttgaa tatagttcct
    53821 taaaataatc cttatataga tgtttatact tttttattaa agatttttgt tcatgatttc
    53881 caattatatg atatacttca tatttttcaa ccaaattatc tattaaatct aatgcaactt
    53941 gaaaattttt attttctcca tctactaaat ctcctgttat aaatataaca tctgggttta
    54001 atgattcaat tttatctatc aataccttat tatttttatc aaacacctta ttatgtaaat
    54061 cactcacttg aactatttta aagttatcaa attcctttgg aatttttttg ttgaaaatag
    54121 tatattctgt cgtttctata tgctgagttt catacttata cagaatatac aataatatta
    54181 atactaatac tgtcattgaa acctttataa tcatattctc ttcctcttta ttctattttg
    54241 ttaatattat tataatgctt ttatgttttt tttaaaacaa taacatcatc acatacatat
    54301 aaatcttttc gacattataa aaataagtta tccttttcta aaattttcat ttattttaga
    54361 aaaggataac tataaaatta tctataaacc tctatattgt gaaatatagt ttttccttta
    54421 tttaaatttt tatatatatg ctctaaagtt ccatcaaacc taatagaatg tccagactct
    54481 aaaatataag ttttcccatc cacattaagg tcaagtttac cttctattac gagtacatac
    54541 tcttctaaaa catgactgtg tgtagatgaa acatgagtac agttttcctc aagctcaatt
    54601 ataaaaagtt caaaccttct ttgagggcta aaagggtata ttggatataa cttcatgttc
    54661 cctttttgct caacaatagg ctctacttct tctttcttta taacctcata ggtatttgta
    54721 ttttcattta atagtgtagt aaaatttatt ttaaatccag atgaaatttt ccataaagta
    54781 gatactgtcg gacttgattc acctctctca atctgtccaa gcattgcttt actgacacct
    54841 gtcatctttg aagctttgtc taaacttatg cccatttctt gtctcaataa ccttaaattt
    54901 tctcccacat taggaaattt attatccatg ttaaaatcct cctgatagct atttgtattc
    54961 tatgattttt actcctgtaa gttctactat ctcttttaat tttaacatat gattgttaca
    55021 aatttcatca aattcatata tataatcttc aagccctaat gccttatatt tatcgattcc
    55081 aaatttatga tatggaagta actccatctt gcctcctgga acattttgtt tgacaaataa
    55141 tgcagtattt cttatatttt cttcactatc atttactcca taaataattg gcactcttat
    55201 aaccatagat ttattcaact ttgaaagcct acatatatta tctaatataa ttttattact
    55261 tactcctgta tattctttat gaatattatc atccatagat tttatatcta caaatatgtg
    55321 gtctattttc tcaaatacat catttacctt attccaatca aaatatccac aagtttcaat
    55381 tgcaatattt atgcctttat cataaaaaat atcaactaat tctcttaaaa aatcaatctg
    55441 aagtgtaggt tcacctccag aaaaagttat ccctccatta gattcaaaga aaaatataaa
    55501 atctttttca acttcctcta ctatttcttc aacactcatt tcttcggtca ttatacaaat
    55561 ggcatcttta agacatacct tgatacactc tccacataaa tcacatttat tatttatttg
    55621 cgctctatca aatagtgaac ttatattttg aggacaaaca tctatacatc tattacagga
    55681 tacacattta tctttcataa cacctaattt tactatgtta ctccaggaat ctggattaga
    55741 acaccactta cattttaatt tacatccttc taaaaatata gtagtcctta ttccatctcc
    55801 atcatttact gaaaaatgtt gtacttttac aactctacct tttttactca taatctacct
    55861 cataattaaa aattgctatg tgaatttcta gctattatag catcttgcat ttctcttgat
    55921 aaattaacaa attgagtact atatccagca actctcacta ataaatcttt atattcttct
    55981 ggattttctt gagcttttaa tagagtatca tttgatatcg tattaaattg tacatggaat
    56041 cctcctaaag caaaatatga tttaatcata gatgttagat ttctaagtcc tctttcagtt
    56101 tccaccaaat cttgattcag tcttaaattt agaagtgttc caccagtatg aacatcatga
    56161 tttatttttg atgcagattt cattgctgct agtggacttg ttgtatcagt accaacaaat
    56221 ggagaaactc cctctgtcaa aggttttgtc gcttttcttc cacaaggagt tgcgcctacg
    56281 atttttccag ttggtacata gtttgatata cccataaatg cactattaaa tttagagcca
    56341 aaaatatcct tgtattttct agtttcagta taataaaaat cagaaacttc aattgctaat
    56401 gaatctacat aatcattatc atttccatac ttaggaacat ccaacgcaag ttttcttaat
    56461 tcttcataac cttcccaatc attatttagt gcttttgtta attcttctaa cgtagtcacc
    56521 ttatcctcaa atactaattt ttttatagct gccaatgaat ttgaaactac acctaaccct
    56581 ataccagtta atacaggacc tatattatat tttgctcctc ctttacttaa atcaactccc
    56641 ttatccaaac atccatctac acaagtagat aaaaatggtc ttggtaccat ttctttatga
    56701 atttgttgag caacagtagt tcctataaca gaacttttta caagatatgc taattgcttt
    56761 aaaaactcat ccttaagttc ttcaaaagaa gtaaattctg tgatattttt tgtatctaac
    56821 cccatttttt ctccagttaa tctactctta ccttcattca tagcatattc taaagctgct
    56881 gcaaaattga tatttacggc tgaagtccac tgtcctgttt ttctaaaatg aggaactaca
    56941 caaccacagt tactccaatc tctagcatct tctgcatcat atccatcttg taataacatt
    57001 tgtgcccctg cactatcgct gtgtatagca gggaaaccag ttccttgttt taccaattta
    57061 gcaactgcca ttacgaaatt gtctggtgct ccttgactca ctctaacact taatcctggt
    57121 tggtgagttt ttacactttc tgtagccttt aaacacatat atgagatatc attcgttcca
    57181 tcagtaccat ttctcttttt accaccaaca gtaagatttt ggaattgatt atatcctgca
    57241 aagtaatttg ctgtatttgc tgatatagtc catacccatt ctgataactt aatccaaagt
    57301 gcttctatca attcttgagc ttctgattca gttattttac cttctctagc atcattttca
    57361 taatatggat acatatattg atcaaatcta cctaaattta atgccaatgg attttctgat
    57421 agaataccac ctaattgaac aaaccataca aattgtattg cttcataaaa attagttgga
    57481 gggtttgctg gtacattcat acatatctct gaaattttta taagttctaa ctttctcttt
    57541 tcatctgttt cttgcttact catctctttt gcaagatttg aatatctttg tgccaatgtc
    57601 ataataccct gtgcaactat agaaacagat ttgtaaaaat ctatcttttc aatattttct
    57661 gaaactgata tatctaattc tttaattttt ttatctgcat cttccttaat ccctttatat
    57721 ccttttacaa acaatatatc ttgataatct ggtgttactt caccaactgc ctgtctccac
    57781 ttagaatcat tatctattat tcctgtatct actaatatct tagctgtatc ttctgggatt
    57841 cttgctaaga aaacttcttc caaagatttt cccttccaat atggaaatat ttcttttctt
    57901 ataaattcta tttgttcttt tgatattaca tatgggtctt gagttctttt atcaaaagta
    57961 tccatttctt tgtctaccca ttgccaagaa aactctggtg tcaatatccc tgttcttcta
    58021 aattttcctg ctgtacctac tattaattca ttttcaaaga tttttatagg aagttcttta
    58081 catgtctcca aaaaagcttt agctcttctt atacagatag cttctccctc agttttttta
    58141 tgtgattctg taaaaattct agccctttca caacatatca taggtttaga attaatatat
    58201 tcttgtctaa atgcttctat tctattagtt tgactcattt aaaatccccc tttggataat
    58261 atatactaca tttttgtatg ttataacata ctttaattat attatactgt cctttagcat
    58321 aaaatggaag caaaataact tatttttttt catctacctt gaacacctat atattaatac
    58381 tttttttgtt aaatttataa aatctaaaaa aattttctat gatatatttt ctatattgtt
    58441 atagttatgt ctaatatact aatgatttga aatatcaaat ttgtatacta gacttagttt
    58501 taaaacctta gttgattgtt taactttgtt aacttcaaca tgtatatcct tcatataagg
    58561 aagctctaca acacctactc ccttattaaa gtcttcttca accatagaaa aaatttcttt
    58621 ctcagttaag ttcgtatcag tcaatgattt aaataaattg tatataaatt taacatattt
    58681 atcactctca gataacttat catcttctgg atactcttta gcacagagaa tatctattgt
    58741 tacttttcct tctttatcgt cttcagacat agaatagaat aaacttcgaa tatttttttc
    58801 aggtggtttt tcagcaccat ttatagatac tacaaagcta ccatctgatt ttgagttagt
    58861 aatactataa ccactgtcta ttatagcttt cgaaaatctt tgagataaat ctttttttac
    58921 ttcttctcta cctttcgcat cattaatctt ttgtgagtaa ttattaaata gctcaataga
    58981 ttcatctttt ttatcttcaa cttgttcttt caatgaatct ttactaattt gttctgagct
    59041 acttaagttc ttatcagata caggagacat acaccctacc atcaatgcta agctaagatt
    59101 taaagccaca attattgcca ctctatttat attccttttt actttcatac acaatcctcc
    59161 catcacacta accgctctaa agtattattc attgcactag aatatattta tattttcttt
    59221 atattaaaga tttattattc aatacatatc accttcctat ccaattctta taacttacta
    59281 ttagtaaaat tataagctcc ttaatacaga aataaggatg ttatcttttg ttattaaaag
    59341 ataacatcct tatttttttg tatcaataca tatgtcccaa ctggtaactt aatcgcccca
    59401 actgtcagta tcttggtcca atggtatata tatatttagt ataaatttat cgatactatt
    59461 ttcaaataat aattctccat tatatttttg taaagaagat ttaatactct gaatacctat
    59521 tccatgtacg aacttatcca ttttgtttgt taacaatata tttttattaa aagtaagctg
    59581 atttagttta ctattttcac atttaagtac aaaaaatgac cttgttatag ttcctttaat
    59641 tctaatatat ttattaatat tttcatccac tattttatca catgcttcta ttgcattatc
    59701 caaaatatta gaaaatatgc ttgacacatc tataggtttt acaaaattta cttttgaaaa
    59761 atttatagag caagtaaatt taattccttt ttttaaacat acttcactct tttcgttgat
    59821 tataatgtct aatatcatat ttcctgtatt atagatatat ttaaatgttt ttatttcatc
    59881 ttttaaattg ttaacatatt cattaatttc tttgctattg tttctaaggt tgtctataca
    59941 ataaatgtga ttatttatat cgtgatatag tttcttaact cgcatatgag attcttgaat
    60001 acttaaataa tgcgcatatt gcatatctaa cttatttttt attaattcag cttgagattt
    60061 taatctattc tcttttatta tttctttaaa caatagtata agaaatatac tactagctcc
    60121 taccaatttt ggattaacaa aattatcaaa atagtatcct tctgaatgta atgacccaaa
    60181 ccgttctaat ttttttaata cataatttac aagcatattc attgtattaa ctaataaggc
    60241 aaataataaa tatacataat ttctcttatc ttggctaatt aattttatag actttctaaa
    60301 aaagctaaat ataagatata taagaataat tattaaatta ctatatatct gaatttttat
    60361 agattctaag taaggtacta ataaagattc tttagatgaa ataacattac ctgtaattgc
    60421 aaaaaatagt attctatata taacagcatc caaacattta taatatacac taaatagata
    60481 aaagaaaatg gaat
//
LOCUS       DAMJYX010000014        59162 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJYX010000014 DAMJYX010000000
VERSION     DAMJYX010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.014"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            219..929
                     /gene="rgaR"
                     /locus_tag="PC479_000940"
     CDS             219..929
                     /gene="rgaR"
                     /locus_tag="PC479_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2803519.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1975..3006
                     /locus_tag="PC479_000941"
     CDS             1975..3006
                     /locus_tag="PC479_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2803520.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3139..4437
                     /locus_tag="PC479_000942"
     CDS             3139..4437
                     /locus_tag="PC479_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2803521.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4551..4796)
                     /locus_tag="PC479_000943"
     CDS             complement(4551..4796)
                     /locus_tag="PC479_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2803522.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4944..5810
                     /locus_tag="PC479_000944"
     CDS             4944..5810
                     /locus_tag="PC479_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2803523.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5814..6350)
                     /locus_tag="PC479_000945"
     CDS             complement(5814..6350)
                     /locus_tag="PC479_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2803524.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6541..6744)
                     /locus_tag="PC479_000946"
     CDS             complement(6541..6744)
                     /locus_tag="PC479_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2803525.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7035..7799
                     /locus_tag="PC479_000947"
     CDS             7035..7799
                     /locus_tag="PC479_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2803526.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8188..9501
                     /gene="prdC"
                     /locus_tag="PC479_000948"
     CDS             8188..9501
                     /gene="prdC"
                     /locus_tag="PC479_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2803527.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      9906..9991
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            10573..12747
                     /locus_tag="PC479_000949"
     CDS             10573..12747
                     /locus_tag="PC479_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2803528.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
     gene            13154..14914
                     /gene="prdR"
                     /locus_tag="PC479_000950"
     CDS             13154..14914
                     /gene="prdR"
                     /locus_tag="PC479_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2803529.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            15343..17223
                     /gene="prdA"
                     /locus_tag="PC479_000951"
     CDS             15343..17223
                     /gene="prdA"
                     /locus_tag="PC479_000951"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2803530.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            17304..17588
                     /locus_tag="PC479_000952"
     CDS             17304..17588
                     /locus_tag="PC479_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803531.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            17626..18351
                     /gene="prdB"
                     /locus_tag="PC479_000953"
     CDS             17626..18351
                     /gene="prdB"
                     /locus_tag="PC479_000953"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:18076..18078,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2803532.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            18431..19189
                     /gene="prdD"
                     /locus_tag="PC479_000954"
     CDS             18431..19189
                     /gene="prdD"
                     /locus_tag="PC479_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2803533.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            19204..19671
                     /locus_tag="PC479_000955"
     CDS             19204..19671
                     /locus_tag="PC479_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2803534.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            19689..20171
                     /locus_tag="PC479_000956"
     CDS             19689..20171
                     /locus_tag="PC479_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2803535.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            20191..21198
                     /locus_tag="PC479_000957"
     CDS             20191..21198
                     /locus_tag="PC479_000957"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2803536.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            21318..22199
                     /locus_tag="PC479_000958"
     CDS             21318..22199
                     /locus_tag="PC479_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2803537.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            22867..23502
                     /locus_tag="PC479_000959"
     CDS             22867..23502
                     /locus_tag="PC479_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2803538.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            23686..24255
                     /locus_tag="PC479_000960"
     CDS             23686..24255
                     /locus_tag="PC479_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2803539.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            24333..24470
                     /locus_tag="PC479_000961"
     CDS             24333..24470
                     /locus_tag="PC479_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803540.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            24467..26242
                     /locus_tag="PC479_000962"
     CDS             24467..26242
                     /locus_tag="PC479_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803541.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            26667..27974
                     /locus_tag="PC479_000963"
     CDS             26667..27974
                     /locus_tag="PC479_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2803542.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            28302..28841
                     /gene="hpt"
                     /locus_tag="PC479_000964"
     CDS             28302..28841
                     /gene="hpt"
                     /locus_tag="PC479_000964"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2803543.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            29219..30895
                     /gene="bclA2"
                     /locus_tag="PC479_000965"
     CDS             29219..30895
                     /gene="bclA2"
                     /locus_tag="PC479_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2803544.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(31402..32157)
                     /locus_tag="PC479_000966"
     CDS             complement(31402..32157)
                     /locus_tag="PC479_000966"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2803545.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            32597..33586
                     /locus_tag="PC479_000967"
     CDS             32597..33586
                     /locus_tag="PC479_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2803546.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(34038..34754)
                     /gene="dapD"
                     /locus_tag="PC479_000968"
     CDS             complement(34038..34754)
                     /gene="dapD"
                     /locus_tag="PC479_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2803547.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(34978..35727)
                     /locus_tag="PC479_000969"
     CDS             complement(34978..35727)
                     /locus_tag="PC479_000969"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2803548.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(35794..36681)
                     /locus_tag="PC479_000970"
     CDS             complement(35794..36681)
                     /locus_tag="PC479_000970"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2803549.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(36798..37799)
                     /locus_tag="PC479_000971"
     CDS             complement(36798..37799)
                     /locus_tag="PC479_000971"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2803550.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(38224..39105)
                     /locus_tag="PC479_000972"
     CDS             complement(38224..39105)
                     /locus_tag="PC479_000972"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2803551.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            39428..40621
                     /locus_tag="PC479_000973"
     CDS             39428..40621
                     /locus_tag="PC479_000973"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2803552.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            40827..41945
                     /locus_tag="PC479_000974"
     CDS             40827..41945
                     /locus_tag="PC479_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2803553.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            42076..42321
                     /locus_tag="PC479_000975"
     CDS             42076..42321
                     /locus_tag="PC479_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2803554.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            42308..43054
                     /locus_tag="PC479_000976"
     CDS             42308..43054
                     /locus_tag="PC479_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2803555.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            43339..44214
                     /gene="hslO"
                     /locus_tag="PC479_000977"
     CDS             43339..44214
                     /gene="hslO"
                     /locus_tag="PC479_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2803556.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            44605..45405
                     /locus_tag="PC479_000978"
     CDS             44605..45405
                     /locus_tag="PC479_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2803557.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            45933..48173
                     /locus_tag="PC479_000979"
     CDS             45933..48173
                     /locus_tag="PC479_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2803558.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            48587..50122
                     /locus_tag="PC479_000980"
     CDS             48587..50122
                     /locus_tag="PC479_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2803559.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            50138..50899
                     /locus_tag="PC479_000981"
     CDS             50138..50899
                     /locus_tag="PC479_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803560.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            51237..52106
                     /locus_tag="PC479_000982"
     CDS             51237..52106
                     /locus_tag="PC479_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2803561.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            52435..52686
                     /locus_tag="PC479_000983"
     CDS             52435..52686
                     /locus_tag="PC479_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2803562.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            52798..53577
                     /locus_tag="PC479_000984"
     CDS             52798..53577
                     /locus_tag="PC479_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2803563.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(53712..54362)
                     /locus_tag="PC479_000985"
     CDS             complement(53712..54362)
                     /locus_tag="PC479_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803564.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(54359..54862)
                     /locus_tag="PC479_000986"
     CDS             complement(54359..54862)
                     /locus_tag="PC479_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2803565.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(54911..55426)
                     /locus_tag="PC479_000987"
     CDS             complement(54911..55426)
                     /locus_tag="PC479_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2803566.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            55706..56233
                     /locus_tag="PC479_000988"
     CDS             55706..56233
                     /locus_tag="PC479_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2803567.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            56217..57620
                     /locus_tag="PC479_000989"
     CDS             56217..57620
                     /locus_tag="PC479_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2803568.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(58003..58314)
                     /locus_tag="PC479_000990"
     CDS             complement(58003..58314)
                     /locus_tag="PC479_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803569.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(58370..58963)
                     /locus_tag="PC479_000991"
     CDS             complement(58370..58963)
                     /locus_tag="PC479_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2803570.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT        22832 a         6977 c        10351 g        19002 t
ORIGIN      
        1 acatattata tttattttac tatatttttt tacataatat atctagaaaa gtataggaat
       61 ttatcgtaat ttaagtttaa atattaaaga gaatttctat aagtagttta atcgacaatt
      121 taaatttata aagaaattta aatttgaaag ctagtgagta cagtttggaa attgaaataa
      181 agtaaactaa atgatatttt agatttggag gtgatattat gttcagggta gttatatgtg
      241 atgacgaaaa aatacaaaga agcatactaa aagagtttac agaaaaactt ttgtctgaaa
      301 gatgtctaaa ttatcaaata ctggaatttt cctgtggaga agatttaata tccaaatacc
      361 cagaaaaaat tgatattata tttttagata tccaaatgaa agatatcaat ggaattgagg
      421 tggctaaaaa aataagaaag tttgatgaaa aagtagaaat tatatttaca acggcttttt
      481 cagagtatgc cccccaaggt tatgaggttc gtgcttatag atatttagta aagcctatag
      541 agtatagtaa ctttgcaatg ggtgtaaatc tttgtattga taaccttcaa agaaagaagg
      601 agcgttatat agttttaaat agtaaaaaag gatttaatag aatactgata gattctattt
      661 tatatgttga aactgtaaag agagatttag tcgtacatac aataggtaga gattataaag
      721 ctaatatgag catgaaacaa gcagaaaaat tactgtgtga aaatggcttt tttagatgtc
      781 atacaagttt tattgttaat ttaagaagaa tagaagatct tagggataat atgattacta
      841 ttaatgaaca atttatacct ataagtaagt atagacttaa agatttaaaa gtagcattaa
      901 caagcttgtt atgtgatata atgtattaat tttaaaaagg taattgaaaa tatcgtaaaa
      961 aagatttaat gtcatcacag ggctattgat tgaaactgtc ttaatatata aaatgttatt
     1021 tatattaaga cagttttatt atgcaaaata gcagtttata gatttttaat atggtttaac
     1081 atgtatatgt agaggttcca ttatagtagc atgattatta attagtagct tgattatcaa
     1141 tatactatta ttgattttta tatgcattat atgtattttt agaatatgat taccttttta
     1201 aaataatgat tatttataaa actctataga tatttgtttc atttagctac gcatacttta
     1261 attaattata ttaatattta atggagtaca atataaatat taaagcgaca tcttatagtt
     1321 tattgaaact atagtaatga attaataaat agtttaattc tatgaaaaga tttcctttta
     1381 tgcaatttat ttattgaaat atgatataat atataaaggt gtaagtatta gttgttaaaa
     1441 taatatttat attagttttt atacatactt ggtataagtt tttcatatat tgattaaaat
     1501 tgatgttgat ttattatttt tagtattttt tcaccttttg attcatactt tgtagaatgg
     1561 tgtattttct aaaactgatt ataagacaag ttcattcaaa ttagcatttt tatatcatat
     1621 aaataattgt tccccctaaa taagatttag atgtatagta ttaaaatcaa aaagtataaa
     1681 ataaaattga tgcttaatca tagataaata ttaataaaat atacaagggg attgtctaat
     1741 aaaaagataa tctccttttt tacaaataat tctaaattgt gatataaatc taaaaatttt
     1801 atcttaacat ttaaccattt aatgataata attgatataa taaatataaa ttaaatatat
     1861 ggtgtactta ttttatgttt ttagcaagaa atatagcatt taaaagataa attgtgcaac
     1921 ttaaatcaaa gtttaagatg aactattaac atagatatat taaaaacttt tgtaatggag
     1981 aatatatgtg atatgaataa taaagttaag gttgcagtat tagatacagg aatagacaaa
     2041 gaacatgatt acttaaaaga taatttagtt ggaggaattg cttttgaatg tatgcatgat
     2101 tatatcttta tatcagataa atttgatgat gaagatggtc atggaactgc ttgtgcatca
     2161 ataataaaga aagagtatga agatgtagag ttatttgtaa taaaaatttt gagaaaagat
     2221 ggtattacaa atataaaagt tcttgaagaa gcattaagat accttttaga cactaacata
     2281 agattaatta atttaagttt atcagtcata ggagtagaaa gtgttaaggg cttatttgaa
     2341 gtttgctatg aattgtttag aaaaggaaaa ataatagtat gttcactcgc taatgatttt
     2401 gacttaagtt atccagctat gtttaacaat gttataggtg taagaggatt tactttagat
     2461 ggagatgatt cattttggta caacaaaaaa tatgatgttc aatgtgttat ggatagcaat
     2521 tcatatataa gttgtgatat aaataattcc tatagattac caccaaaatg taatagttat
     2581 ttatcagcaa agcttactgg taaaatagct aagatattat cagaaaaacc aggcataaca
     2641 atatctgatt taaatgataa gcttgaatca ttagcaacta gaaatcattg ggatagttgt
     2701 gattttgatg agtgtagtag aattgtaaac tttaaattgg atttgtatga caaagaaaat
     2761 gctcttttta tgaaagttgc tgacgttgtt agggattgct tagatattga agagaataat
     2821 gaaaaactct ttaagggtag cctttttaat aaggagatag gcttggttta tgataattgt
     2881 tttaatctgt tgaaaaagct agagcataga tttgacataa agtttaatta tatggacatc
     2941 tcaaaatatg acttagtttc aatatataca ctgactgaat tagtggaaag atataaaaac
     3001 ttataaataa agatttgtag ctaaatttaa tgtagaaggt attaaattat atgtattctt
     3061 tgtcggtaga ttaaaagtaa aaaatatact ataattagat agcataagag aaaatgccaa
     3121 tacaggggga gttagataat gaaatatgaa gaagcattag agtacatatc acaaactaat
     3181 aaattcggta taagattagg attagaaaat atcggaaagt tattagaatt attaggaaat
     3241 ccacaagaaa ctttgaatat aatacatgta gctggaacta atggaaaagg ttctgtgtgt
     3301 tcttttgttt ctaatatact aagagagtgt ggatataaag ttggtttata tacatctcca
     3361 tatttagaaa cttttactga gcgtataaga gtaaatggac aaaacatacc acaggatgat
     3421 gttgctagaa taattgagtt aataaaagaa aaaatagaga ttatggtaaa agaaggctat
     3481 gcatatccga cagaatttga agtggttact gcaatggcat tttattacta ttcagaacaa
     3541 aaggtagact ttgtggcatt agaagttggt ttaggtggca gatatgatgc tacaaatata
     3601 ataacaaaat cacttgtaag tgtgatagta tctataagtt tggaccatac aggtatactt
     3661 ggagatacaa tagaaaaaat agcgtacgaa aaggctggaa taataaagga aaatggagta
     3721 gtactagtat atgaccagac tgatgaagca aaggatgtta taaagtcagt ttgtaaagag
     3781 aaaaaagcta aatatataga agttgatttt gatgatataa acataaaaaa atcagatata
     3841 aattctcaaa tatacgattg tactgtaatg aaagaaactt atagagacct tgaaataaaa
     3901 ttgataggcg aacatcaaat aaacaattct atcttggcaa ttagtgtaat aaaatatcta
     3961 aaagacataa ataaattagc aaatataaat gaagaatcta taagaaaagg acttataaat
     4021 actaaatggc caggaagaat agaaaagata aaggaaaatc caatttttat aatagatggt
     4081 gcacataatg aagatggagc aaaatctttg gcaaaagcac ttgataagaa ttttaaaggt
     4141 agaaaattaa ccttattaat aggtatgtta gaagataaag atattgatag tgttttagag
     4201 atattgttgt cacattttaa taaggtaata acaacaacac caaataaccc aagggccata
     4261 aattcagata tattaagaga aaaagtatta aaatatgtag atgatgtaac atcaaagcat
     4321 gagatagagg atgcagtaaa ttatacatta gaaacatcaa gtgaggatga tattataata
     4381 agtgcaggtt cattatatat gattggtaca gtgagaactt tagtaaagaa attataattt
     4441 tataaaataa gtatacaaat aaaaatataa aaaagagttg tctaaaaaat ggacaactct
     4501 ttttatataa aataagtttt taataatttt actgatgttt atataagtaa ttatatagat
     4561 tgacgtatag ctttagataa ttggtctggg caagaagtac cttttccacc acagtcaata
     4621 ccatttaatt tatcagcaac ttcaagagca ttttggccaa ttattaagct tctaagtcct
     4681 gataaattac cattacatcc tcctataaac tcagcatcaa caatcacatt attttcatca
     4741 acttcaaaaa tcatttctct acaacataca ccacttggat taaatgttac tttcattaaa
     4801 aagacctcca ttaaatatta tttctttttt taacattaat aataatacca aatatatgcg
     4861 taataatgca actataaagc ataatacaag ctaatgaaat aatatattat tgtagtatat
     4921 gtattgtaga gaggaggggt tatatgaaaa tctatatttc taactactta tcaaagagta
     4981 ttagcatagt tgattattct acacttgaac tagaaaaaga aatagtatta gaagataata
     5041 tatatccaca ccatttttgc atagaaaaag aaaaaaattt aatatacata cctagttcaa
     5101 gtaatgggat actctatgtc ttagatttaa gtaatgataa aataattgat actgtctcta
     5161 taggaggaag tcttagtcaa gttgtactta gcgataatga attgtttgtg gcaaatgaag
     5221 attctaatag tatatatgta ctagataaaa acacattaaa tccaatagga attattggag
     5281 ttgataatat gccacatgga tttgactttg atagagaaag caaaaagtta tatgtaccgt
     5341 gtataaactc gatagtatgt atagatacaa ttaataaaaa tatacaaaag aaaataaata
     5401 tggactttag agcttggcat atagaacttg ataaacaaaa gaaagaaatg tatatatcaa
     5461 cacttgatgg aaagcttata ataatagatg aagtaagcat ggatataaaa aaggtattat
     5521 atgaattttt attaccagta gaaataagat ttaattatag tggcaaaaaa gtttatgttg
     5581 ctgatttggg ttataataat gtaagaattt tagattatac aacgagtaga tacattggaa
     5641 atattgaaat aaatgggatt ccccaaggat tagaaatatc taaagatgaa aagttactat
     5701 ttgtatcaga tacacaagaa aattcagtaa aagtttatga aactgcaaat aacaaattaa
     5761 taaaagaaat caaggtagga aaagagccta caactataat ttgtttgtag gctttaatgt
     5821 atgtctctta taagtaaatc gataacttcg gcatacatat aagatgcatt atttttccag
     5881 ttattgcaaa taagttttgc ttgtttctct gatgaaacat ttagatttaa atctattaag
     5941 ttagactgat tttctatgac tctaaggttg acaataaatt cattatcact ttgttttaca
     6001 aaactagact ttacttgagt atctgctagt agattttctt tgttagaatc tatatacttg
     6061 tctatttttt cagtaacact agtaggtatt cgattaaaga agtattctag actttctacg
     6121 cctctttgag taagagcata atattctctg tcagagtctt tatatgtagt caaaaactta
     6181 gattccataa gctgagataa aagctgttgt aatgaaaaat aattcatcat ctcagtctct
     6241 aatactactt gagttatttg agaattagtt aaatccattt taattttttc taatatatat
     6301 aaaattaaaa gtttgtgata ggctaattct tcagacgagt tttcaaacat aaatatcatc
     6361 ctccgtcaaa ctatttctat atataatata ttaaactaat ttgtacaaaa ttaaaagaac
     6421 aatttgtatt gtattattaa tctttaataa ttcatataaa aacaatttta taaataaata
     6481 tagaaaaaca gggctgctaa ttagcagccc tgttgatacc caggtgtcaa aatttactat
     6541 ttattttcca gccatttgtc tttcagccat ttcaactaat ttcttagtca tatatccacc
     6601 aacatatcca ttttctctag ctgttaagtt acctttgtct atgttttcat agttagataa
     6661 acctatttcg ttagctattt ctaatttcat ttggtttaaa gcagcttttg cttctggaac
     6721 tactgttctg tttgaatttg acatagtaaa tttcctcctc aaataattag tttatgatgt
     6781 ctaacaacac ttttatttat gtgatgttaa tactaatttc tccaaaataa aaataatata
     6841 tacagaaaaa taaaggtaaa aatgtaaaaa atataaaaaa attaaataaa tgagtatttt
     6901 agagagttta attaggtcaa tataggtttt atttttttag ttattgtctt ataaattaaa
     6961 tattacagta cttttttgag ctgaaaaaaa ataaatttta aatatagaag aaattatttc
     7021 aaagggggaa tagtatgaaa agagaagtta aaaggactaa agctttgata tatagtgttt
     7081 tatttttttc attattatta gtgttaatat ttgtttttgt ttatggaata aaatacatat
     7141 gttctaaatc tgacaattca aaaatgccta tatataaagt tgatacaaat gagaaaaaaa
     7201 tagctttgag ttttgatgta gcatggggaa ctgataatat ggatgatatt ttaaaaattt
     7261 tagataaaca taatgtaaaa gctaccttct ttttagttgg tagttgggtg gatgataatg
     7321 aagaaattgt aaaagctata gatgaaaaag gacatgaaat tggaaatcat tcaaatactc
     7381 atgccaattt gaaggaaata tcaaaagaag acataacaaa agagatagaa actacatcag
     7441 aaaaaatata taatataaca ggaaaaagga ctaatttatt tagacctcct tttggggacg
     7501 taaacaataa agctatggac atttgtagtg atttaggtta taaagtaatt aagtgggatg
     7561 tagattctat agactggaag gagcttggac caaatcatgt aattgaaaaa gtaataaaag
     7621 aatctcaacc tggttcaata gttttattcc atgctaatat aaatgatgta gataactatt
     7681 tggatactat aataagtcga ctaaaaaaag atggatacag tctagtcaaa atatctgatt
     7741 tgttgtataa agataattat gtagttgatt ccaatggagt gcaaaaaact aagaattaat
     7801 aagtaataaa tgtatatttt aagtaaagtt gtgataaatt atatagtata tgttagaaaa
     7861 gtgtcgataa aaagacggtg tctatttttt gacacttttt atacatgtat gcaatatcaa
     7921 taattataga ttaaattgat taaaaaagtg tctaataatt gacttttttt tgatgggttt
     7981 aaaaatttag aagaattaat gaatgctgta agacagtatt tccaaagggt ataagtttgt
     8041 ttaaaagttg gcatagaaat tgctttgtta ataagcgata tgttctatag aaataaacaa
     8101 tatgttaaga ccttataata gaaaaatagc attaacatta tttgaacagc ttataaaaaa
     8161 attaatatac ttaagggggc gtactagttg aacaaactat attctatttt attaaaacaa
     8221 cacgttggtg gtccagataa gccagtagta agtgttggag atgtagtaaa aaaaggaact
     8281 ctaatcgcag aaccaactgg attaggggca aatatttatg ccagtgttag tggaaaaata
     8341 agtgaaataa atgaccaagc gatagtaatt gaagctgatg aagtacaaga agatactttt
     8401 gaaccattaa aaggagaagg tatacttgac ttaattaaag aagctggagt agtaggaatg
     8461 ggtggagcag gattccctac tcatgtaaaa ttaaacatag atttaaatgg tggaacaata
     8521 ttagcaaatg cagctgaatg tgaaccttta ttagctcata atataaaaga aatagaagaa
     8581 agaccagaaa tagtttatca aggtataaaa tatgctatgg aagttactaa tgctggaaaa
     8641 ggtatgctag caataaaaag taagcatcca aaagcaatag aagctttcaa aaaagtaata
     8701 aagccaggag acaacataga agttgcagaa ctagttgata tgtacccaat gggagaagag
     8761 agagcaatag taagagacgt tctaggaaaa ttacttgagc caactcaatt accttcagag
     8821 gctaatgcag tagtaataaa cgtagaaaca ctaactagaa tagtagaggc tgttgagcaa
     8881 aaaagaccag ttatatctaa aaatataact gtagttggtc aattaaacag tggaaaagaa
     8941 tctatagtgt ttgaagatgt gccaattgga acaactgtcg gagaattaat tgaaagagct
     9001 ggtggaataa aaggtgaata tggtgaaata atcctaggag gacctttcac tggtaaagct
     9061 actactttag atgctccaat aactaaaact agtggtggta taatagttac tatgccattt
     9121 gttaatgaaa agagaaaaat gggtctttta gtttgtgcat gtggacctaa tgaagaaaga
     9181 atgagagata tagcaactaa aatgggagtt actgatatag tatcagttca aaaatgtaaa
     9241 caagctcaag aaattaaagg tgcattaaaa tgtgaaaatc caggacactg tccaggacaa
     9301 gctcaaaaat gtattgaatt taagaaagct ggagcagaag ttatattaat aggaaactgt
     9361 actgactgta gtaacacagt aatgggttct gcaccaaaat taaaattagg tgtatatcat
     9421 attactgacc atgtaatgag aacagttaat cacccattaa taagaagaat aaaaggtgac
     9481 gtaagattaa aaataaaata atagataaat agaagttaga catctttaga agtaaaactt
     9541 aaaagttact tctaaggatg ttttttaatg tattttgagg gcaatatata tattgtaaaa
     9601 taaatggaaa aaatagacaa aatatgctag atattgtata atgataataa tagctattac
     9661 aattaatatt ggacaaaaat aatgatactc aagtatgtgg tttgtattaa tatgcatatt
     9721 atattaagtc aactatccga tagagtaaaa ggtttttgtg atatttgaag atactctata
     9781 gtatcttttt tattataaaa actaattttt gtcttttatt agataagtat ctaggatact
     9841 aatttatatt ttattgagtt gttaaaaaag tgcacttaaa aagttaaaaa agaaaaatta
     9901 aaaaacctat ttatagaaac tgtgaagtat atcttaaacc tgggcactta aaagatatat
     9961 ggagttagta gtgcaacctg ctataaatat aaataggagt aacttttaat tgtcaaaaat
    10021 atataaaata aatacataaa taagtaatta atagaagata gatataaata tatttttgaa
    10081 atgctccctg cattcataac gagtgtagag tggcagatat ctgcccaaaa ctcctttaat
    10141 tgctggaagt tcctaaagct aactaaacca caacataatc atgaaataag gataagtgtg
    10201 atagagtgtg catttaataa gcacttgagg ttacgaaagt agaaaaaatt agttagatag
    10261 cataaggtta aatcctaagt gccttgaaaa tggataatca gcaggtaagc ttcgaaaaga
    10321 agaaacttca acgactagag agaaatctcg tacctacaag tgtggggaaa tggggagtac
    10381 ctaatttatg gttaggtaat gatatagtct gtgcttaata gaaatattaa gaagttcata
    10441 ggagaactgg ttaggagtaa cgaccctaat tgaacacact aaagatgtat tattgaataa
    10501 gattcatata atatttaata attttaatta tattaagtga atacaatata tgtttttagt
    10561 atcaggagaa ttttgaaaac aaaaattaaa aaatcaagta taatcagttt agtaattgct
    10621 tttagtatgg tgtttactgc atttacacca atagtatctt atgctgacga agtgactagc
    10681 aatgatacaa tacttaatgg agaagaacaa tcaaatggtc agacaccaga tgtagagaaa
    10741 ccatcagacg gtcaggtacc agatggggag aaaccatcag acagtcaagt accagatggg
    10801 gagaaaccat cagacggtca ggtaccagat ggggagaaac catcagatgg tcagatacca
    10861 gatggggaga aaccatcaga tggtcagata ccagatggag agaaaccatc agatggtcag
    10921 ataccagatg gagagaaacc atcagatggt cagataccag atggggagaa accatcagat
    10981 ggtcagatgc cagatggaga gaagacatca gatagtcaga cgccagatgt agagaaacca
    11041 tcagatggtc agatgccaga tggggagaaa ccattagatg agcaaactcc agaagaagaa
    11101 aaaccattgg aagaagaaat agttatagag gaaatgagtt taaaacaaga tattgataaa
    11161 attcttgata tgactttatc tcaaataaat aaaatagtat ataacttttg ggaagatgaa
    11221 gaagatgtaa aggcagatga acaatcagaa attaaccaag tttttacttc agaagactca
    11281 tttattagct tatggtatga caaaaaagca aaagtaaaaa attcttgttt actaaaagaa
    11341 atagatggtt cagatagacc ttatcatgat ttaagatttg atgatattac aaagacttta
    11401 acttttgact atgtaataaa gggtcttgta ggagcaagct caaaagacga caaatatgta
    11461 atagaaggtg aagatggaga gcaaactgca ttttgttaca ataatcattt aagaccacca
    11521 agtagtaatg gaaagagtcc atatttacca gcagaagatt ttaatggtca agaaaaccaa
    11581 aatgaagaag cagttaaatc tatactatat gcaggttctg aatttgatgg atttggatat
    11641 aagcaacaat ttaatttagg tggagaagaa aatgaattga tgacttactc agctactcaa
    11701 agtgcaattt ggattattct tggacaaatg gatgaacaag aaagacttaa gcagtatcaa
    11761 ggttctataa acctttgtga taaacttata gaaagagcaa aaactgaaga ggaaagagct
    11821 gagtatcaga agaagaaaga agtagctata aatataaaag cttatttaga agcattgtta
    11881 aaagcaggtc gtgaagagtt aaagccaaat gatacaggaa aacctagttt gagtaatggt
    11941 ttaactaaga ttaactttga aaaaaatgaa gatggtactt atgaaacaga agctgtagcc
    12001 ttagtaggtt atagtggtgt agtaaagtta agactaccag atggagtaac tgcttatgat
    12061 ggagatggta atataatagg tacaggtgaa gtagaaattt caacacaaca gaaatttaaa
    12121 cttaagagtg ttgtaaaacc agatgccaaa gctaatattt ctgctgtatc ttatgattat
    12181 atttttccaa aagctattca atattataag gcagtgcttg atttaggaca aaaagaccat
    12241 tcatcttcat taccatctag taagcaaaat ttattaagtt atacaataga gaaaaaaaat
    12301 ggtcaagaag ttaactttaa tataggtcta ccaacagatg atgttgtaaa tccaccagtg
    12361 ccaccaacag atgatacaaa tggacataag ccaaaaccat caccaccaat agatgataca
    12421 gtaataaatc caccagtacc accaatggat gatacaataa taaatccacc agtaccacca
    12481 acggaggatg cagtgttaaa tccaccagtg ccaccaacgg aggatgcagt gctaaatcca
    12541 ccagtgccac caacagagga tacagtgtta aatccaccag taccatcagt aagtgataca
    12601 gtggaaaaaa ctccagaact atcaagagat gatacaataa taaaatcacc aaaaacaggt
    12661 gatgaaactc agctaatgtc atatgtattt attagtgtca ttgcaatttg tggattagcg
    12721 tatcaatgta aaataaaaag aaattaatct agattattat taagtttaaa ataaaaagtc
    12781 tcaaattgat ttataagtca ttttggggct tttttatgta aatataatat gcaaatatag
    12841 tttgtttaaa aatatatatt ttaattatat tcttaaattt atgattggat acaggcattc
    12901 attaattgtt tgatgaatag aaaaatgttt tgaaggtgaa aatagtattg aaaaaataat
    12961 atactaaggg tataattcat ttgatttcat ttgtataaat ttaattataa attttaaagt
    13021 gattatataa atttaaatgc taattactta ataaatttta ataataatta tttaataaat
    13081 aattattata atatgctaga ataaatatat ataaataagt tttgatgagg tactactata
    13141 aaggggggac tttatgtttt cgataccaga agtaaaaaaa gtagaagagg taatggatac
    13201 aaaatttaca actatagacg aagacacaag aattgaagat gcaataaaag aaatgataaa
    13261 aagcaacaca aaaactctaa tggtaataga ttcatctgac caattaaaag gcattatatc
    13321 catgacggat atacataatc tttatgagat gcataaaaag tatgaaggtc aaccagtaaa
    13381 acttataatg aagaaggatg taatatatgt caacgaaggt ttaaccttag atgaatgtag
    13441 agatataatg atattaaaaa atatagggat tcttcctgtt cttagagata ataaaattat
    13501 tggagttcta aaacaagagc acattaggga ctatctatat atgcatttag aagattatgg
    13561 attaactttg aagtatatta taggtcaaat taaagaagga atttgtgcta taaataacga
    13621 aggtgttgtt atcttgtgga ataagtttat ggaggaaagg tatgatataa agtctgaaga
    13681 tatagttggt cgaccgatga atgaattttt ggaaaatact atttcagaaa aagtactaaa
    13741 tagtaaagtt ggaatgagtg acttgtattt tacagataaa aaagaaaata tgtatgcatt
    13801 agttcatgca aatcctatat tttataaaga agaatttatc ggtgttgtat gtacagaagt
    13861 tgatgttaca gaagctaaga tattagctct tgaacttgaa aaagtaaatg atacattaaa
    13921 atatctaaag aacgaagtca aaaatttatc taaaggtagt tttgataaga ttttaggtaa
    13981 aagttataaa ttggaaaaat cgaaagcgat tgcaaaacaa gtggctagaa caaatagtag
    14041 catatttatt tggggcgaaa gtggtacagg taaagaagta tttgcaagag ctattcatga
    14101 ttatagtgag agaaaaggtc aatttatacc tgttaactgt agtgccatac caaatgagtt
    14161 atttgaaagt gagttttttg ggtatgaatc aggtgcgttt acaggagcaa gtaaaaaggg
    14221 tcgaataggt atatttgaac ttgcaaaaga tggaactgta tttttagatg aaattgccga
    14281 tttacctctt agtatgcaag caaagttgct tagagtatta caggaaaaag aaataagaag
    14341 agttggtgga gataccacaa taaaaataaa tcctagaata atatcagcta ccaataaaga
    14401 tttgagtaaa atggtaaagg cagaaaaatt tagagaagat ttatactata gattaaatgt
    14461 tgttgaaata aagattcctc cacttagaga gagaaaagaa gacataggat tattggttca
    14521 tagcttttta gaggagatat gtaaacaaaa taataagcct gttcttacaa ttagcaaaga
    14581 tgtaatagat atatttcaga attatagatg gaaaggtaat attagagaat taaaaaatac
    14641 gattgaaaat attgttgtat tgtcacaaaa ttctaaaata gatgtggatg atgtaccaag
    14701 ttatatgatg gattctacaa ataatagtac tgaagaagaa gagtatcctt tagatttaac
    14761 gaaggcaact caaaaaattg aaataaagaa tataaccaag gcacttaaaa tgtctaatgg
    14821 aaataaagct aaagcagcta agatattaaa tatacccaga acaactttgt attataagat
    14881 agaccaatac aaaatagatg tgtctaaaat atgacacatg actaaatttt gacaaaatga
    14941 taaactttaa tttaaaacct agtaaagcca acgcttatat aagtgttgtg tatatagatg
    15001 tgttaaaaaa aatgaaaaag tgttgattaa ttgacacttt ttttgtttga gaaaatgttt
    15061 acaaatacaa aaaataacat attttaaata aatatttata taatatttgg ctaaaaatag
    15121 cgatagatga agaatctatt atagaaaaaa tctttttgtt aataagttgg cataggaatt
    15181 gcttataata taaagtgact ttaagtgaga aaaaaataat attaatcata acaaattatg
    15241 taggttttaa tttacttaat ttctcagtta aggggttacc tgttgaggaa ttgagattta
    15301 aaataataaa taagaaaata atgtaaggag gtattttata ctatgtcaat aactttagaa
    15361 acagctcaag cccatgcaaa tgacccagca gtttgttgtt gtagatttga agcgggaaca
    15421 attatagcgc cagaaaactt agaagatcca gcaatatttg cagacttaga ggattctgga
    15481 ttattaacaa taccagaaaa tggattaact ataggtcaag tactaggagc taagttaaaa
    15541 gaaactttag atgcactttc tccaatgact acagataacg tagaaggata caaagcagga
    15601 gaggctaaag aagaagtagt agaagaaaca gtagaagaag cagctccagt atcagaagca
    15661 gcagtagttc cagtaagcac aggagttgca ggtgaaacag ttaaaataca cataggtgaa
    15721 ggtaagaaca taagcttaga gataccttta tcagtagctg gtcaagcagg agttgctgct
    15781 ccagtagcaa acgttgctgc tccagtggca agtgcagcag cagaagtagc tccaaaagtt
    15841 gaagaaaaga aacttttaag aagcttaact aaaaaacact ttaaaataga taaagttgaa
    15901 tttgctgatg aaactaaaat agaaggaact actttataca tcagaaacgc agaagaaata
    15961 tgtaaagaag ctaatgaaac tcaagagtta gttgtagata tgaagttaga aataataact
    16021 cctgataaat atgaaactta cagtgaagct gtattagata tacaaccaat cgctactaaa
    16081 gaagaaggcg aattaggttc aggtataact agagttatag atggagctgt aatggtatta
    16141 actggtacag atgaagatgg agttcaaata ggtgaatttg gttcttcaga aggtgagtta
    16201 aatactacta taatgtgggg tagaccaggt gctgctgaca aaggtgaaat attcatcaaa
    16261 ggtcaagtaa caataaaagc aggaactaac atggaaagac caggaccttt agctgctcac
    16321 cgtgcatttg actatgtaac tcaagaaata agagaagcat taaagaaagt tgacaactct
    16381 ttagtagttg atgaagaagt aatagagcaa tacagaagag aaggtaaaaa gaaagttgtt
    16441 gttataaaag aaataatggg acaaggtgca atgcatgata acctaatatt accagttgag
    16501 ccagttggta cattaggagc tcaaccaaac gttgacttag gaaacatgcc agttgtatta
    16561 tctccacttg aagtattaga tggtggtatc catgcattaa cttgtatagg acctgcatca
    16621 aaagaaatgt caagacatta ctggagagag ccattagtaa taagagctat ggaagacgaa
    16681 gaaatagatt tagtaggtgt tgtatttgtt ggttctccac aagtaaatgc tgagaaattc
    16741 tatgtatcta agagattagg tatgttagtt gaagctatgg aagttgatgg agctgtagta
    16801 actactgaag gtttcggaaa caaccatata gatttcgcat ctcacataga gcaaataggt
    16861 atgagaggta taccagtagt tggtgtaagt ttctcagctg ttcaaggtgc tctagttgtt
    16921 ggtaataaat acatgactca catggtagac aacaataagt ctaagcaagg tatagagaat
    16981 gaaatattat ctaacaacac tttagctcca gaagatgctg ttagaataat ggctatgctt
    17041 aaaaatgcta tagaaggtgt agaagttaaa gctcctgaaa gaaaatggaa tccaaatgtt
    17101 aaattaaata acatagaagc tatagaaaaa gttacaggag aaaaaatagt attagaagag
    17161 aatgagcaat ctctaccaat gagtaagaag agaagagaaa tatacgaaaa agacgaaaac
    17221 taaaatttag atactaacta agatttagac aataacataa atttagatcc aagtttaaaa
    17281 cagtatagag aggtagaatt ataatggata ggttaaaata tatctcatca gagacttttt
    17341 atgaaggtat tatagttgac atcaaaggcg gtggagtgac tatagaccta aaaggtagac
    17401 ttggacaatt taaaatacct aatagaatgc ttataactga ctacgaactt aagataggtc
    17461 aagaagttgg attcatgcta agctatcctg aggtattaag tccagagcct aatcaagagt
    17521 atgtagaaaa tattaggaga gaaaaagaaa aacaagcaga aatgcaatca aaacaagaag
    17581 aaaaataata aataatcgtt tgaaaataga agggagagga aatatatgag ccttacaaca
    17641 gtacaaggac ttcaatctga aatattcgtt ccaataacac ctcctccagt ttggactcct
    17701 gtaactaaag aattaaaaga tatgactata gctttagcta cagcagctgg tgttcattta
    17761 aaatctgaca agagatttaa tctagcagga gatactacat ttagagctat acctaataca
    17821 gctacagttg atgaaatgat ggtatcacat ggtggatatg acaatggaga tgtaaacaaa
    17881 gatataaact gtatgttccc tatagataga ttacatgaat tagctgcaga aggatttatc
    17941 aaaggagtag ctcctatgca ctatgcattc atgggtggtg gaggaaacca acatgtcttc
    18001 actgaagaaa ctggtcctgc tatcgctgct aaacttaagg aagagggagt agacggtgta
    18061 gttatgacag ctggctgagg tacttgccat agaactgccg tgatcgtgca gagagcaata
    18121 gaggaagctg gaatacctac aataataata gcagctcttc ctccagtagt tagacaaaac
    18181 ggaactccta gagcagttgc tccattagtt ccaatgggtg ctaatgctgg tgaaccacat
    18241 aatatagaaa tgcaaacaca tatattaaga gatacattag agcaattagt tgcaatacca
    18301 tctgctggta agatagttcc attaccatac gaatataaag ctcacgttta aaatgtaatt
    18361 tatatttgaa aatatagaac ccttctccta cacggttagg agaggggttt taagaacaaa
    18421 ggtggatatt atggaagaaa aaataatgag aagacttgtt ataaaaccgt ttcatatgaa
    18481 tgaagttaat ttcggttcaa aaacttctat aaagaaagat gtacttacaa tagatttatc
    18541 aagtatagat gaaataaaag aaagagaaga gttaataaca gacataaaag tagacattat
    18601 aaaacctgga gattatgaca gagaaattaa tactattatg gacattatac caatatcaac
    18661 aaaagtttta ggcagactgg gagagggtat tacacatact ttaacgggtg tatatgtaat
    18721 gttaactggt gcagatgaag atggaaatca aatgcatgaa tttggttctt cagaaggaat
    18781 tttgaaagac caaatggtat ttgggagata tggaactcca tcagttgaag attatataat
    18841 ccatgtagat gtaactttaa agggaggttt accttttgaa agaacacttc cacttgcagc
    18901 attcagagca tgtgatgatt ttatacaaga aataagagcg tctttgaaaa tggaagacgg
    18961 aagaaatgct actcaagttc gtgaatattt tgacaaaatt cgaccaaatg caaaaaaggt
    19021 tgtcatagta aaacaaatag caggtcaagg tgctatgtat gacaatcagt tattctcaaa
    19081 ggaacctagt ggatttgaag gaggaacatc aattatagat atgggaaatg tacctatgat
    19141 tatatctcct aatgaataca gagatggtgc ccttagagct atgacttaag agaggtggat
    19201 attatgggaa taggaccttc aacaaaagaa acatctcttc atcactttag agacccactt
    19261 ttagacatag ttgaaagtga taaggatgta gatttacttg gagtaatagt agtaggaact
    19321 ccagatggaa atgaaaataa aacctttgtg ggacaaagaa cggccgcttg gctggaagcc
    19381 atgagagtag atggagctat agtttcatca gatggttggg gaaactcaca tgttgactac
    19441 gctaacacat ttgaggaaat aggaaaaaga gatatacctg tagttggagt tacatttaat
    19501 gggactcaag ctaagtttgt tgtaagcaat caatatatgg ataccatagt tgatatgaac
    19561 aaatcaaaag aaggtattga gacagaagta gttggagaaa acaacacaaa tgaaatagat
    19621 gctaaaaaag ctttggcatt tttaaaactt aaaatgagaa aacatggata aaaaatttaa
    19681 gtaagggaat gagttttgtg ggaatttcta catcaatgaa aaacacatca cttcatatat
    19741 ttagagaccc gttactagat ttgatagata atgattctga catagacttg ttaggagtgg
    19801 ttgtagttgg gacatcagag cacaatgaat ggaaaacatt tttagcaact agacttggaa
    19861 gatggattga atcattgcga cctgatggag tgattataac tttagatatg gcaggaaacc
    19921 agcatattga ttttacaaat gctatagcag agtttgtaaa atcagatata cctacagtag
    19981 gtcttactat tatgggagca gatggattag taattaccaa tccatatttg gataaagcta
    20041 ccataattga ttataaaaag acaacaggct acatagaaac agaagtagtt ggagacaatc
    20101 acatggatga agttgatgtt aaaaaagcat tggctttcct aaaattaaaa atgcgtaaag
    20161 acgcaaaata aacctaaggg ggaatttgat atgaaattta gcagaagtat acaagctata
    20221 gactctcata cagcaggtga agcgactaga atagttgtag gtggaatacc taacataaaa
    20281 ggaaattcta tgcctgagaa aaaagaatat ttagaggaaa atctagatta tttaagaact
    20341 gctataatgt tagagccaag aggacataat gatatgtttg gttctgtaat gactcaacct
    20401 tgttgcccag atgctgactt tggaataatc ttcatggatg gtggcggata ccttaacatg
    20461 tgtggccatg gtacaatagg agctatgaca gcagctatag aaacaggtgt agttccagca
    20521 gtagagcctg taactcatgt agttatggaa gctccagcag gaataataag aggagacgtt
    20581 acagttgtag atggaaaagc taaagaagta tcattcttaa acgtaccagc tttcttatat
    20641 aaagaaggtg ttgaagttga cttaccaggt gttggaactg ttaaatttga catatcattt
    20701 ggtggaagct tctttgctat aatacatgca agtcaattag gtttaaaaat cgaacctcaa
    20761 aatgctggta aattaactga actagctatg aaacttagag atataataaa tgagaaaata
    20821 gaaatacaac atccaacttt agctcatata aaaactgtag acttagttga aatatatgat
    20881 gagccaactc atccagaagc tacttacaaa aatgtagtta tatttggtca aggtcaagtt
    20941 gacagatctc catgtggaac tggaacaagt gctaaattag ctactttaca tgctaaaggt
    21001 gaattaaaag taggagaaaa attcgtatat gaaagtatat taggaacttt attcaaaggt
    21061 gaaatagttg aagaaactaa agttgcagat ttcaatgctg tagtacctaa aataactggt
    21121 tctgcttata taactggatt taatcatttt gtaatagatg aagaagaccc acttaaacat
    21181 ggatttattc ttaaataagt aataaataaa ttaggaaatt aaaatagaat agtagccact
    21241 tgtcggttgc tattctatag cttttaggct ataagattca gaatatatga aaaaataaaa
    21301 aaataggagg atggattatg gtagcaatct taaaggcatt atatgtagta ttcgcagcaa
    21361 tctttgcagt tgtgtttggt aaagacgtag ctaaagcaaa caaagagggg aaactagaag
    21421 aacaaagtac tccaaaagtt gctgtaacag gatttataac agacttcttt gatacattag
    21481 gaatagggtc atttgctcca acaatagcaa tgtcaaaggc tttaaagtta aatataccag
    21541 ataaaaaaat gcctggtaca ttaaacgtag cacatactat tccagttgtt acagaagcat
    21601 tcatatttac atcaattatc ccagtagatg gtgttacttt agtatctatg gttgtggcag
    21661 cagcagttgg ttcatatata ggtgcaggaa taatagctaa gatggacgag agaaaaatac
    21721 aacttgttat gggtgtaact ttagcattaa cagcaatatt aatgttatta ggtcatcctt
    21781 ggattaacgt attaccaggt ggaggaaatg aattaggtct tactggtgct aaattagtaa
    21841 taggtgtaat aggtaacttt atactaggag ctttaatgac agctggtata ggtctatatg
    21901 ctccaggtat ggcaatggtt tatttcttag gaatgtctgc aaaagttgca ttcccaatag
    21961 ttatgggttc ttgtgcatta ttaatgccag ttgcaagtat gaaattcata aaagaagatg
    22021 catatactaa aaaagcatca gttataatag ctatatgtgg tttaatagga gttttcgtag
    22081 ctgcatatat agtaaaaact ctaccaatgg atatattaaa agcattagta atagtagtta
    22141 tagcttatac ttctgctaca atgataatgg ctgcaaataa aaacaaaaag actgcttagt
    22201 taaagactac ttagtgttta ttaaatatta cagctaataa taaagattat aaactgcttt
    22261 aaaatattga attaatattt tagggcagtt ttttatttac taaattaata taaagcaatt
    22321 aacatgccaa ttatgtaata tatatagtga tttagtagat ttaatctaaa aaacgttgta
    22381 tttttaaaga ataaagcata atttaaagct aaatatttaa attttataat caataagttc
    22441 aataaatgtt catatattaa tacagattaa taaaaatata acctgtaata agtctaatat
    22501 aacttataat taagaaaaat aagtttataa tgactcaata ttcatctgaa ttatttcaat
    22561 aaatttaatt cgttgaagtt gcttatatag agtgaataaa tttaagttat atatgactta
    22621 ttaattatat aataggattt ttataattaa tacaaaactg aaataggtag tttaattagt
    22681 atatgtggta caaaaagata ataattttat aattataaaa gtgaaatatt aaatgaaatt
    22741 tataactaaa taaaaacttt taaacagcaa tcaaagattt gtaataaaat acatccctta
    22801 taaatatatt tattatataa gctgatacca gaaatataag aattaacaat tgtggaggga
    22861 taaaatatga tagctgttaa agatgagaat aacgtagtaa acttaagagg ggagttagac
    22921 aataagctag aatttagcca tgagatattt ggagaaaaat tttataatat gaaaataaaa
    22981 ataaacagac taagcgattc ttttgatata cttccaatga ctgtatcaga aagacttctc
    23041 caagatattg accttgataa acaaaattta gtcaatgtcg taggccaatt aagatcatat
    23101 aacaaaacat taaataataa aaatagatta gtgcttacag tgtttgtcag agaaataaaa
    23161 agtatagacg aggaaaataa agaccctaat agtatatttt tagatggata tgtatgtaag
    23221 gaacctgtgt ataggaagac acctttagga agggaaataa ccgatttatt agttgctata
    23281 aacagacctt ataataaatc tgattatata ccatcaatag tttggggtag aaatgctaaa
    23341 tttgcaaaga atctaaaggt tggtgataga atacagcttt ggggtagggt tcaaagtaga
    23401 gaatatgaga aaaaaataga tgaaaataat gtggtcaaaa aaatggcata tgaagtatcc
    23461 atatcgaaaa taaaaaaatt ggatgaaaat aacaatgaat aaacataaaa aatagactga
    23521 tttttatgaa taaaatccta ataaatttgg tataatatca tattaaaatg tataaagatt
    23581 gtttaaaaaa atactaatga aatatctctt gaatagttgc tcgccattta ttataatgta
    23641 atctgtacat aatttataat tgaggaaatg aagaggtgaa gcattgtgaa aagcatatta
    23701 tgtcagataa ataaacagcc agttaaagct aaatatacat ctgatgaatt agaatgtttt
    23761 ttggatacaa ttgagtataa tgtaggtgat aaaatattgc ttgttggtca tataggaaac
    23821 ctaggcaaga gattgagagg tcttggaact tctgtaacta tactggagaa tagtaattat
    23881 gaagatgtct gttattctct agtgtataat gaaaattgca atgtagttaa aggtaagtta
    23941 gagtttctac catttgatga taatcagttc aacaagataa taatagttga ccaatttaat
    24001 gttattacag attgtgaaaa agcttcatct gaaatacaaa gagtgttaaa gggcaatgga
    24061 gaggttatat tagaggattt aaatttaaaa aatttaaaag ttaagttaaa ctatcttaaa
    24121 cataaatttt gtggttctgt tgcaaagatt cactatccac atgaagtatt taatatattt
    24181 tcaagattaa actttgatgg tatgattaaa gagtcaaata aattaaaata tatttatgtg
    24241 ggaaaaagaa gataaatgag ttaaaatata aatatggata taatataaaa gactaggtta
    24301 agcctagtct tttgttataa ggagatgttt agatggaaat aaattatgag ttaataataa
    24361 ttgttatgct ctttgtaatc ctgatgtcaa tccaatttac tttaaataag atatacttga
    24421 ttcttaaaga aataagacaa atacttatgc taaagaaaaa taaggaatga tgattatgat
    24481 aggtttagaa tatataaaat ctgatattca aaatatagct gaagctatag tatcagtact
    24541 caatatagat gtaactatag taaataagga gttatttagg attgcaggta caggagtata
    24601 tatagacaaa ataggtgaga aagtagataa atatacggct tttaaaaaat cacttacaga
    24661 acagattaca atactaattg atgacccaaa gagtagtgat atatgtaggg aatgctataa
    24721 aagtggggct tgtgtagagt ttgcagaggt gtgttgtcct attatatgtg atggcgattc
    24781 tcatggtgtt ataggcctta tagcctttac aaaagaacaa gcacaaatta tagaaaataa
    24841 taaaagtggt cttataaact ttttaggcaa aatggcagac ctaatatcta ataaattaaa
    24901 agcacaaatt aaaacttatg agcttgaatt agaaaagaaa aaattagaga cactattaaa
    24961 caatatggac aaagctgttg tgtctattga tgtggatgga aaaattgata aatataattc
    25021 aaaatttaaa gagcttttca atataaaaga tagtataact ggcaaaaaca tatttgtcga
    25081 gttagatttt ataaagaaac cttctataaa taactttaag aaggacaaat cctgtagttt
    25141 tttttataaa agatacggat atgattcgaa gggaatatac aacatcaata aaatagtatt
    25201 aaaaaatgag ttaaagggat atgttattga ttttatagat aaaagagatg ctataaaaaa
    25261 ctacaataag atgaataaag attatagaat aaaattggaa aacataattg gagaaagtgt
    25321 agctataaga aattctaaaa aggaagccct aatagcttct aaatcaacat caacagtact
    25381 tattactggg gaaagtggaa cgggtaagga gctatttgca agagccatac acaaccatag
    25441 caatagagta gataatcctt ttatagctgt aaactgtgct gctataccgg ataatttatt
    25501 agagagtgaa ttatttggat atgaagaggg agcttttact ggagctaaaa aaggtggaaa
    25561 gcttggaatg tttgaagtag ctcataaggg aagtatattt ttagatgaaa taggagatat
    25621 gagtttgcat ttacagtcaa agttattgag agtattacaa gaaaaggaac ttaataaaat
    25681 tggaagtaaa tcaaatgttt caatagatgt aagaattatt gctgctacaa ataaagattt
    25741 agaaagaatg gttcaaaatg gcacttttag agaagattta tattatagat taaatgttat
    25801 acctataaaa ttaccaagtt taagagagag aaaagaagat atcccactta taatcaagca
    25861 tatggttaaa gagtattcaa aaaaattaga taaaaatgtt attgacatag atagcagggt
    25921 tgttgatgct atgatggaat ataagtggcc agggaatata agagagttgc agaatattat
    25981 tgaatatagt gtgaatatgt cttcttcttc aataatcaca atgaatgtaa ttcctcaaaa
    26041 aattaagaat acctctgtag aagagttaga agttaaaagc gataggataa ttccacttga
    26101 tgaactagag aagattgaga taataaaagc tttaaataag tataaagatt ataaaaaaga
    26161 taaagaatta acagctaaag cacttggaat atcaagagct acattatata gaaaaattga
    26221 aaaatacaat ataatttcat agtaatataa taaaaagcta tataaaattt aaatttttct
    26281 aaattcataa ctttaaagaa agctttaaac ttatactata agtgttttgc agaatctttt
    26341 tttatgaatt aaaaaatggt ttttataagt taaaaaataa tatatttgag ccgtctcaaa
    26401 atgatataga atctcatttt gagacttttt tttgtctttt tgcgaaattg tcatatgttt
    26461 ctccatttta aagaatataa atactctata caaataaaaa aaatacataa atagaggcag
    26521 tcataccaat ggttatagaa tataagacta aaaatataaa aagatataaa aaatatggca
    26581 tgtattttgc tataaatata gataagaaat tatttaacta attatttgag tactaaatct
    26641 aaacttaatt gggaggttta tatataatga gagatataag aaaagaatta gtggaaatgt
    26701 taaaggcaga agttaaacca gcagtagggt gtactgaacc agtagcctta gctctagcat
    26761 gtgcaaaggc aaaggaatta ttaggagaag aaatagttga aaatcgtatg ttagttagtc
    26821 ctagtatcta taaaaatggt atgtgtgtag gtataccagg tactgaaaga ttaggattaa
    26881 aaattgcagc agctctaggt atagtaggtg gacattctga aaatggatta agtgtgttag
    26941 aaactctaac aaaagaggaa gttaaaatag ctgaggatta tatggataat actccactat
    27001 caataacacc tgctgataca agagagaaag tatttataga agttgtgtta aaaggaaaaa
    27061 atcatattgc taaagtgaga ataagaacta aacatgataa ttttacgttc ttagaaaaag
    27121 atggagaagt attattagac aatgaaccaa aagtatcagc ttcaaatgat gcggcagaaa
    27181 aagctgaaag tttaatggat acagtaacta tacaagaatt aataaaaaat gtagaagaaa
    27241 ttgattttaa agatatagaa tttttactag atggagtaaa aatgaatgaa gagatggctg
    27301 aatatggatt aaaacaaaaa actggtattg gtgtcgggta tggaattaaa aagtctatag
    27361 aagaaggtct tttaggtaat gatgttataa attatgcaat gatgttaact gctggagctt
    27421 cagatgcaag aatggcaggg gtaaaaatgc ctgttatgag ttcaaatgga agtggaaatc
    27481 atggattaac agcaattctt ccaatcgttg cttataataa aaaattccca caatctgatg
    27541 aaagactagc aaaagcttta gctatatctc atctagtaac tggttatatt aagaactata
    27601 caggaagatt atctgctgta tgtggatgtg gtgttgctgc ttctactgga gcaactgctg
    27661 gtatctcttg gcttatgaat ggtactgaaa agcaaataga aggagctata gaaaatatga
    27721 tagctgactt aagtggaatg atatgtgacg gtgcgaaagc tggttgtgca ttaaaattat
    27781 cttctgcagc atctgcagct atacaaagtg ctataatagc taagcaagat tgttttgtac
    27841 caccactaaa tggtatagtt ggttcaagtg ttgagcaaag tatacaaaac ttaggtagag
    27901 taagtgacaa aggtatgtct ataacagatg agataatctt aaatgtaatg aatgatatga
    27961 ataaagttga ctagtacttt ataatatgtt ttgtattatg aggtataaca aaaatagcat
    28021 aaataaattt aattagtacc ctctaataaa atatgattat gtataaattt atgtataata
    28081 gaaaaaactt gttagtgcaa gttttttctt tttttgtcta aaatatatag tatgtaattt
    28141 agtgtggata tatgaactac ttaaaatata agatacattg ttaataattg ttgaatgaac
    28201 ataaaaactc aataatattg actaatattg aataagagta ttgaaaaagt gagcaatctc
    28261 ttataaaatt aagaaggatt attacaattg gaggtctttt tatggatata gaaacaaaaa
    28321 aatgggaagt attgtattca gaagaaaaaa ttaaagataa attaagggaa ttaggagcaa
    28381 ttatagaaaa agattataaa gataaaaatc ttatggtagt ttctctatta aaaggtagct
    28441 ttatattttg tgcagactta gttagaaata taaatttacc acttagagtt aattttatga
    28501 ctacatctag ttatggaaac aatgaagaaa gtactggaag ggttaaagta gtttcagatg
    28561 tgactactga tatagcagga tatgatgttt tggttgtaga cgatattact gattctgcac
    28621 ttactatgga ttttgtgtta aaacatttaa aagctaaaaa tcctgctagt ttaaaatgtt
    28681 gtgttctttt ggataaacca agcagaagaa aagtcgattt agtgccagat tattgtggat
    28741 ttgaaataga agataagttt gtggttggat atgggtttga ttttggtgat tattatagaa
    28801 atgttcctta tatattcaat gttactgatg aagatagata agcaattata taattttgtg
    28861 gatgccttaa tatgattttt attaatcgtg ttgaggtttt tttatttaca aagtaattaa
    28921 agtagtatta aattaatttt atgcaattta attatctatt ttttataaca taataaaaaa
    28981 attttaaata attgatggaa aatgagtttt ttaattgtat taataattgt aaaagagtac
    29041 taataattgt aaaagatttt acaaaaaagt aatgaaattc aagaatcaaa gcatatatgt
    29101 tgatataagc aaatagatac attatcacat aagaaattgt cgattcatat aattatatag
    29161 agtaatactc aatatttata gagtaatact ctaactttaa aaaaggagga ttaattatat
    29221 gagtgatatt tcaggtccaa gtttatatca agatgtaggt ccaacagggc caacaggtgc
    29281 tactggtcca acaggaccga cggggcctag aggtgcaact ggagcgaccg gagcaaatgg
    29341 aataacaggt ccaacaggaa atacgggagc aaccggggcg aatggaataa cgggaccaac
    29401 aggaaatatg ggagcgactg gagcaaatgg aacaacaggc tctacaggac caacaggaaa
    29461 tacaggagcg actggagcga atggaataac aggtccgaca gggaatacag gagcaaccgg
    29521 agcaaatgga ataacaggtc caacaggaaa caaaggagca actggagcaa atggaataac
    29581 aggctctaca ggaccaacag gaaatacagg agcgactgga gcaaatggaa taacaggtcc
    29641 aacagggaat acaggagcaa ctggagcaac aggtccaacc ggactaactg gagcaaccgg
    29701 agcaaccgga gcaaatggaa taacaggtcc aacaggaaat acaggagcaa ccggagcaaa
    29761 tggagtaaca ggtgctacag gcccaacagg aaatacagga gcaacaggtc caaccggaag
    29821 tataggagcg acaggagcaa caggaacaac tggggcaaca ggcccaatag gagcaaccgg
    29881 agcaacagga gcagatggag aggtaggtcc aacaggagca gtaggagcaa caggtccaga
    29941 tggtttggta ggtccaacag gcccaacagg cccaaccgga gcaactggag caaatggttt
    30001 ggtaggccca acaggcccaa ccggagcaac tggagcaaat ggtttggtag gtccaacagg
    30061 agcgacagga gcaacaggag tagctggggc aataggtcca acaggagcag taggagcaac
    30121 aggcccaacg ggagcagatg gagcagtagg tccaaccgga gcgacaggag caacaggggc
    30181 aaatggagca acaggcccaa cgggagcagt aggagcaacc ggagcgaatg gagtagcagg
    30241 tccaataggt ccaacaggtc caaccggagc aaatggagta gcaggagcaa caggagcgac
    30301 aggagcaaca ggggcaaatg gagcaacagg cccaaccgga gcagtaggag caacgggagc
    30361 aaatggagta gcaggagcga taggaccaac aggcccaact ggagcaaatg gagcgacagg
    30421 agcaacaggg gcgaccggag caacaggagc aaatggagca acaggtccaa ccggagcgac
    30481 cggagcaaca ggagtgttag cagcaaacaa tgcacaattt acagtgtcct cttcaagttt
    30541 agtgaataat acattagtga catttaattc atcatttata aatggaacta atataacttt
    30601 tccaacaagt agtactataa atcttgcagt tggagggata tacaatgtat ctttcggtat
    30661 acgtgccaca ctttcacttg caggatttat gtcaattact actaacttta atggagtagc
    30721 tcaaaataac tttattgcaa aagcagtaaa tacacttact tcatcagatg taagtgtaag
    30781 tttaagcttt ttagttgatg ctagagcagc agctgttact ttaagtttta catttggttc
    30841 aggcacgaca ggtacttctg cagctggata tgtatcagtt tatagaatac aatagtttat
    30901 atgatgaaaa gattataaaa taataaacaa tatgatttca tataccaata aatagaataa
    30961 tatttaatag gtttatatga tgaaaagact ataaaataat aaacaatatg atttcatata
    31021 ccaataaata ggataatatt taataggttt atatgatgaa aagactataa aataataaac
    31081 aatatgattt catataccaa taaattagga taatatttaa taggtttata tgatgaaaag
    31141 attataaaat aataaacaat atgattccat ataccaataa ataggataat atttaatagg
    31201 tttatatgat gaagagatta taaaataata aacaatataa ttccatatac caataaataa
    31261 gataatcttt aagaaaaatg attatattta aaaggtaaac ataaaattta taaactttta
    31321 gaatagaaat ctcaatcaat tagatattca aattgatgaa tatgaatacc taaatagatt
    31381 gagatttatt atgtacaaaa cttatcctaa tatatctgac attttataaa gcccagattg
    31441 cttaccaatt aaaaatttag ctgcaactat agaaccgttt gcaaatactt catcagattg
    31501 agaatgatgg cttaatgtaa gaacttcatt atcacctgca aatacaacat catggtcgct
    31561 gattatattg ccacctctta tagagtgtat tccaatttca tttggttttc ttttacaatc
    31621 tctaccatat ctaccatgtg tattttcact ttcaggcaag acttctctaa ttgcattagc
    31681 tatcatagta gcagttccac taggggcgtc ttcttttcta ttgtgatgtt tttctatgat
    31741 ttcaatatca aaaccttcta acatctttga agcatcctta actaatttta ttaatatatt
    31801 cactccaaga gatgtatttg atgataataa tataggaatg ttttttgatg cttcttctat
    31861 tttatttaag tcattttcat catatccagt agtagctaat actacaggtg tattattttt
    31921 tagagcatat gacaatattg gctctaaagt ctcatgatgt gaaaagtcta ttattacatc
    31981 agttttttca gttacatcag tcattgtttt gtaaagcttt gtatcactat gatgaccttt
    32041 ttcatcagca gacctagcta caccacaaac caactctaat tcattatctt tttttacaca
    32101 ttctgctaaa actcttccca ttgttccatt atagccacta attataactt tcaacataaa
    32161 ttcctcctat gaaattaata aaaatttgtt atatttctat aagtttattt tatcactaat
    32221 ataatttata taaaataata ttagtgataa taagcttaca atttatctta cattaaaaaa
    32281 tttaagtgga tttttcaaaa tatagataaa tttctttttc attgtataaa tgataaaaat
    32341 atatttgact tcataccaaa aagagtgccc catgaactaa aaagttaatt ttgaaatacc
    32401 atattcatat atcatatgat ttatttggaa atcaatatta atacattaaa ataactaaat
    32461 acatgtatgc ataaagatgt ataaaaaata tataaacata tagtattaat tttaaattca
    32521 acaatttaat tgttaaaagt gttattatta aattagaaat atcttaactt aatatactga
    32581 gatgggagtg atagatatgt taaaagaaga aaaaacttat caagagaaag acgcaaaagt
    32641 attcaatgga gtggcagctt tgataatgat tttattattt ttagtagttt caataggtat
    32701 catggttttt gggatagtaa aacttgatag tggatttact ggaataggtt cacttatgtt
    32761 aattttggga cttgtattta tagtagtaga ttttatccta ttttttgggc taaaaatgat
    32821 taatcctaaa gaagctatag ttctagtttt gtttggtaac tattatggaa ctataaaaaa
    32881 agaagggtac tattgggtga atccattttg ttcagcaata aacccagcag tatcaagaat
    32941 taatgtatcg aaatcttcat ctgatgaaaa ggtagatata tcaactaatt caaggggcaa
    33001 aaaagtatct ttaaaaacaa tgactcttaa taatgaaaag cagaaagtca atgatttatt
    33061 gggaaatcca attataatag gagtggttgt aatttggaag gttatagatg caacaaaggc
    33121 agtatttaat gtagataatt ataatacatt cttatctatt cagtgtgatt ctactattcg
    33181 caatgtttca agactttatc catatgatgt atctgaagat ggagatgaaa aatctcttcg
    33241 tggtagtagt caggaaatag cagatagatt aaaagatgaa ttacaatcaa gggttgatat
    33301 tgcaggaatt gaagtgtgtg aagtgagaat tactcatctt tcttatgcac cagagattgc
    33361 agcagcaatg ttacaacgtc aacaagcaga ggctataata gcagcacgta aaaagattgt
    33421 tgagggggct gtaagtatgg tagagatggc attgactaat ttaagtgaaa ataatgttgt
    33481 gactttggac gaagaacgta aagcagctat ggtaagtaat ctgttagttg tgctttgtgg
    33541 caataaagat gcacaaccag tggtaaacag cagttctatt tattaaatta taaaaagttt
    33601 aatagtttat tttattgata tatagaaaat ttagtagttc attttattaa attatagaaa
    33661 gattaatggt ttgctttatt gaattataaa aagtttaata atttattcta ttgatatata
    33721 gaaaatttag tagttcattt tattaaatta tataaagatt aatggttttc actttattaa
    33781 attataaaaa gtttaatagt ttattacatt gatatattga aaaattgaag gaaataaaaa
    33841 tacttgtcag caagaaataa aaatgatata agaatgaata agagaattgt aaaaaaataa
    33901 aatgattata aataaaagat aaaaatattg taaaaaagag ggtgcctctg gtgaattaaa
    33961 ttcacacaga gacacctttt taattaattt tttaaattag tatattattg tttaattaat
    34021 agtatagtac tatttaatta atctaaattt ctaaggtctt ccataagctt agttttgtca
    34081 gccgtttttt catctttcat ttttataact ttagcaggag aaccagcaac aacggcacca
    34141 gcttctacat cagtagttac aacagcacca gctgcaacaa cagcaccttt accgattcta
    34201 acaccttcaa gaataactgc attagctcct attaaaacat catcttcaac tataacagga
    34261 gtagcagaag gtggctctaa aacaccagca acaacagcac cagcacctaa gtgaacattt
    34321 ttaccaagag ttcctctagc tccaataaca gcattcatat ctaccataga accttcacca
    34381 ataacagctc ctatatttat aacagctccc atcattacaa cagcattttt ttctatagta
    34441 accatgtctc ttattattgc acctggctca attcttgcat gttcacttaa agtatttttt
    34501 agaggaatag cagaatttct tctatcatat tctacatgag tatctgttat atcatttcca
    34561 tgtttatcta atacttccat tatatcctca tagtcaccta caaatatata agaaccatta
    34621 cttccgaata ccttaaattt atcagttgat tggaagtgta aagagttagt atttacatat
    34681 accttaacag gtgttttctt tttaacttct tttatatacc ttgctatttc ataagcatcg
    34741 tttaaattta ccatttgtta attctccttg tatttcatta tatttctttt attatatact
    34801 tatttgtcaa ttttagtata tacttacttc ttattattta tattttattt tagttagttt
    34861 ttattgtata gacatagtat taaataaatt tattatatat gtttgatttg tattgcgtat
    34921 tattatatct atattgcata tgtttaattt atattgtgta ttattatatt tatgctatta
    34981 tcccaacatg ctgtccatat tataaaatcc tggtttttgc ttaactaagt atttggcagc
    35041 agctatagaa ccacttgcaa agataccacg agattgagct tgatggctta ttgtaagaac
    35101 ctcattgtct ccagcaaata gagcatcatg ttcgccaaca atatttccac ctcttatagc
    35161 atgaatacca acttcatttg gttgtctttt tgcactacga ccatatctac cataattatt
    35221 ttcaacgttt ggcaaaacct cttttatagc gttagcaatc ataacagctg tgccacttgg
    35281 ggcatctact tttctgttgt gatgtttttc tattatttct atatcaaatc cttctagtaa
    35341 tttagctgca tctttaacca attttactaa tacatttaca ccaagagaca tattatatga
    35401 ttggaataca ggaataactt tagccgcttc acgtattttg ttcatttcgt catcattata
    35461 tccagtagta gcaattacta aaggtgtttt agtctttaaa acgtaagata agatattatc
    35521 taaattagag tgatgagaaa aatctataac tacatcagct tcctcaacta tagtactcat
    35581 atcttcataa attttaaggt cgccttcata gctatccaga cctctagctg caacacaaac
    35641 caattctaat tcattatctt ctcttatagt atcggctaaa atcttaccca tttttccatc
    35701 ataaccagtt gctataactt ttaacataat ttcctcctat gattaagggc taacttttga
    35761 ccaaacttac tttttaccat gcattaatct ttactatgca ttaactttta gcccaaagtt
    35821 aactaattct ttctttaata cttctaaatt tgctgggtcc atttctgcta gaggtaatct
    35881 taaatctcca acattgaatc ccaataaatt cattgcagtc ttaactggta taggatttac
    35941 ttctataaat aaagctgcga ttagagcatc cataccaagt tgtaattttc tagaaccttc
    36001 aatatcgcct tcaaagaatt tagctaccaa atcgtgagtt tcttgagggc aaacattagc
    36061 aagtactgat ataactcctt gacctcctaa agataataaa ggaagtatag tatcatcatt
    36121 accagagtag atagcaaagt catctggaac taaactagct atttctgcaa cctgtcctag
    36181 gtctccacta gcttctttta cagcaactat gttatcaatt ttagcaagtt caactataag
    36241 gctaggactt atatttacac aagttcttcc agggacattg taaagtataa ttggaagttt
    36301 aacactgtta gctattgttt caaaatgacg cttaagtcca gctttattag ctttattgta
    36361 ataaggtgtt attattaata gagcatctac tcctaatttt tcagcttctt gacttaagtt
    36421 aactgagtgc atagtatcat tagaaccact tccagctata acaggaactc ttttattaac
    36481 tttttcaaca acatacttaa tagtagcaag ttgttcttca tcagtcatag tattagcttc
    36541 accagtagta ccacaagata ctatagcatc agtaccatta gctatttggt attcaactaa
    36601 ttcacctaat ttgtcaaaat caactttgtt atcctttgta aatggtgtaa ctagagcaac
    36661 agcagaacct ttaaatatca tataaaatca atcccctctt ttttattttt gtgttttata
    36721 ttacagttac ttctaatgct tgtttttgag ttactattaa tattttacaa aattatatat
    36781 ttatacaagt ttgtatatta tactaaatcg tatgataaag ccatttcagc tatttgaatt
    36841 gcgttagttg cagcaccttt tcttatatta tcagcaacaa cccaaagatt tacaccatta
    36901 tcaacactaa aatctcttct tattcttcca acaaacactt catctctatc atcaaattct
    36961 acagcagttg gatattcgtt tttagcagga ttatcaacta ctacaacacc tggagcttct
    37021 tcaagtgttt ttactaaatc atctaactca aatggattgt taaactcaac atttatagat
    37081 tcactatgac cattttttac tggaactctt acagtagttg cagttatttt taaatcgtag
    37141 ttatcaagta ttttcatagt ttcatttacc attttcattt cttctttagt gtatccattt
    37201 tcagtgaatg aatctatatg aggaagacag ttgtaagaga tttgatgagg gtagaatcca
    37261 gtaggattac cgttcattcc tttctctaaa tcttcaacac ctttaacacc agaaccagat
    37321 acagcttggt aagtagagta aacaactctc ttaattttgt atttatcatc aagagcttta
    37381 aggggaacca tagcttgtat agtagagcag ttaggattag ctattatacc cttatgattt
    37441 tttatggctt ctggattaac ctcaggaact actaaaggca catctggatt cattctccat
    37501 tgactagagt tatctactac taagattcca tttgaagcag ctattggagc atacttttca
    37561 ctaatacttc caccagcaga gaataatgct atttgtatgc ctctatcagt aaatgaagtt
    37621 tcctttaatt cttctactat atattcttta ccacaaaaca ttactttaga accagctgat
    37681 ttagaagaag agaataaaaa tagattttct attggaaaat ttctttcttc taatactttt
    37741 aaaaatgttc tacctaccat tccagtagca ccaactattg ctacatttac ttttttcata
    37801 taaattcacc ccaattttaa attattaaaa cattatttta ttttagttat acaagtaaga
    37861 tatgttaata tttttatata tccaaaaact ttaatattat attaacaact ttaattatat
    37921 acaaaaaacg tacaaaggta tactttgtac gtctaattta tcactcaaag taaaccctgt
    37981 agctcatcac ctacaaaaat aagtgacagt attacacatg ttatgtgcaa tcccagaaaa
    38041 tattattgca agaaatattt tcttcggctg taaatccttt ttccaatcat taacacctgc
    38101 cagctactat tggatactct tagttacagc tacctctacc ctaggttaat aggatattaa
    38161 aatttgatta ttctaataat aatacatatt taagaaaaaa tcaacttatt tatataaata
    38221 ttgctaaaat ttaaatccaa aatttgtcaa ttcttgtttt aacacattaa gattagtttc
    38281 ttccatttca gctagaggaa gtcttaaatc accaacatta aaacctaaaa tgttcatggc
    38341 agttttaaca ggaactggat ttacttctat aaataaagca gcaattaaag catccatatc
    38401 aagttgtaat ttttttgagc cttcaatatc accttcaaag aatttagtta ctaaatcatg
    38461 tgtttcttta ggacatatgt tagcaagtac agagataaca ccacttccac ctaatgataa
    38521 tagtggtagt atagtatcat cattaccgga gtatatagca aagtctttag gtactaattt
    38581 agctatttct gcaacttgag ctaagtctcc acttgcttcc ttcacagcaa ctatattatc
    38641 tattttagca agttcagcca caacagatgg ctttatattc actttagttc ttcctggtac
    38701 gttgtataaa ataattggca acttaacgct atttgctatt gtttcaaagt gaagtttaag
    38761 acctttctca ttagtcttat tatagtaagg agttattatt aaaagaccat ccacccctaa
    38821 tttttcagct tcttgactca tatgaacaga caacatagta ttgtttgaac cagtacctgc
    38881 tataacaggt attctcttat taactttttc aactgtatat tttattacag caaaaatttc
    38941 agattcactc atagtagtcg cctcacctgt tgttccacaa acaactagtg catctgtgcc
    39001 attctcaatg tgatattcga ttaattcacc taatttttca aagttgacat tattatcctc
    39061 tgtaaatggg gtaactaaag caacagcaga acctttaaat aacatataat caaatctcct
    39121 tttttatcta aatttaattt tttaagtaca ataataatta tagtacaaat taattaattt
    39181 taataggtat agttaatatc ttaactgttt aaataagata tttatattat attaatatta
    39241 gaaaaatttt aaataaatgt tttattctca aaatatgcat attaatttaa taaaatatac
    39301 caaaatggaa ataaaaaata aattttatga gaatttaata actaataatt aatatattta
    39361 ggttataata aatatgtgcg taaaatctaa caataagcaa taataccgta aatttaggag
    39421 gaataatatg gatactttaa aggaactttt taaaatagga agtggacctt caagttctca
    39481 tactatggga ccacaaagag cagcagagag atttaagaat gaaaacccag atgcagaaag
    39541 ttttagagca atattatatg gtagtttggc agctacaggg aaaggtcact taactgacta
    39601 tataattgaa aaaactatag cacctaaaaa agtcgaaatt gtttgggaag aagatattat
    39661 aaaagatttt cacccaaatg ggatgaagtt tgaagcttta gataaagata aaaatgtaac
    39721 agctgaatgg actgtatatt ctgtaggtgg agggactata gcagaagaag gtcaaagaaa
    39781 cagtaaaagc aatagtatat atcacttaga tactatggat gagatagtaa aatggtgcaa
    39841 agaaaataat aagactttgg tagattttgt gttggaatgt gaaccaaaag acataaaaga
    39901 ttatataaaa actataaaag atgctatgag aaaatctatt gatgatggac taagtacaga
    39961 tgaaataata ccaggcaagt tattattaaa aagaagagca agtacatttt ataatgcata
    40021 taaaaaagat aagagcttct caacacttgt atatgcatat gcactagcag cttcagagca
    40081 aaacgcttct ggtaatataa tagttacagc accaacttgt ggttctgctg gagtaatacc
    40141 aggaatattc tttgcaatgc aggatttcta taattatgat gacgaaaaaa taattgaagc
    40201 actacttgta gctggaataa taggtaatat aataaaaact aacgcttcaa tatctggagc
    40261 agaggtaggg tgtcaaggtg aagttggagc cgcttgttct atggcagcag cagcagtagc
    40321 atacttaaaa ggtggaacta tagaccatat tgaatatgcg gcagaaatag cacttgaaca
    40381 tcatttagga atgacttgtg accctgtata tggatatgtt caaattccat gtatagaaag
    40441 aaatgcaatg gcagctcaaa gagcttatga tgcagctaat tatgcattat taactgatgg
    40501 ctcacattct gtctccttag accaagttgt tgaaacaatg aaagaaactg gtatagatat
    40561 gatggataag tacaaagaaa cagctaaggg tggacttgct aaacatttct tctcttgcta
    40621 aaaataaaat tttccaataa taattcctct gttttgattt atgaaacagg ggaattatta
    40681 ttaatggtga attatttaat aatgcaaaat atttttatat tcaatttagc tttttatttt
    40741 taattagtat aacatgctat ataatatttt aattataaag aaatatagaa atatttttta
    40801 ataaattaga aattggagga gattacatga gagaggcatc atatttacaa gaacaacttt
    40861 ataaacatag gaagtcatta aatcctattg cagagattgg gagaaaagag tataagactt
    40921 caaaatatat aagagattat cttgataaat taggaattga gtatgaagtt tatttagaaa
    40981 caggtatagt aggtgtaata gaaggtaaaa gacctaaaaa agatattgct tttagagctg
    41041 acatagatgg tcttcatagt gaagaagggg taaaacattt atgtggtcat gatggacatg
    41101 ctactatatt acttggatta attgagtatt taaatgataa taaagaagac ttaaatgata
    41161 atatagtatt tatattccaa ccagcagaag aaggtcctgg gggagcaaaa gctttaatag
    41221 atgaaggagt gcttagaaaa tacaatattg atgaaatata tgggcttcat atatatccag
    41281 agttaccaga aggttatgta ggtacaagag agggctattt tatggctcaa ataggagata
    41341 ttgacataga aatagtgtct aaaagtggac atggggctat gccacaaaat ggtattgatg
    41401 gtatagtgat agcttcaaac tttgtaagct cacttcaaac tatagtgtct agaaatataa
    41461 gcccaataga caatgcagtg ttgactattg gtagaattga agggggagct agaagaaata
    41521 taatagctga aaatataaag atggaaggta ctatgagatg tttcagccca gaagtgtatg
    41581 agaaaatgaa gcttagagtt agagaacttg ctagagggtt tgaattggct tataactgta
    41641 aggttaatat aaatattata gatgattata ttgctgttaa aaatgacaag gatttgtatc
    41701 aagagtttgt agaggctatt ggaagtgata ctgttgtaga gttagagcca cttatgatat
    41761 cagaagattt ttcttattat caaaaagaag ttccaggatt attttttatg ttgggttcaa
    41821 gaaacgaaga aaaaggattt gtaaatggtc ttcataatat aaactttaat tttgatgaaa
    41881 agatatgtgt aaatgcattg aatgtttatt caaaattatt aaaatacaaa gaagcaatag
    41941 attaggaaga taagttataa agattaaaaa gcataaatac ctaaatttaa aaaaagtgta
    42001 ttaataaata ccccttacat atttgtataa atttatttta attgacaaaa atacaaataa
    42061 gaaggggtga tttttttgaa agaaaaagta caaagagaaa agaagataac taatggcaag
    42121 aatactaaaa tattgacaaa tgatgatata atgaaatatg agatagcctc agaactaggt
    42181 cttatggata aagttggtga acgtggatgg gcaggactta cagctaagga agcaggaaga
    42241 attggaggta tgcttacatc acgcaaaaag aggcttaaaa agattgtgga ggagcagaat
    42301 aaaaaggatg agtcaatata gtgattttgc gtttgtatat gatgaactta tgaatgaagt
    42361 tgattataat ggttgggtta aatatataga agatataata gatagtgaag gtgtaaaagt
    42421 aaaaaatata ttggaattgg catgtggcac gggtaatctt acaattccat tgactaaaaa
    42481 aaattacgat atagctggta tagatatatc tgatgagatg cttagtgtag caagagaaaa
    42541 ggcagaaaaa gaaggagtag aattagtttt acttcaacaa gatatatcag agcttgattt
    42601 tgaaatatct gatttggact gtgtactttg tgcttgtgat ggatttaatt atataactta
    42661 tgatgatgat ttggaaaatg tattttcaaa gacatatgaa ttattaaaga aagatggaat
    42721 attcatattt gatataagtt ctttttataa attagccaac atattgggaa ataacatgta
    42781 tggagaaaat agagaagata tagcgtatat gtggcaaaat tactttgatg aagaagagaa
    42841 tctaattgaa atggaactta cattctttat taaagatgaa tgtggaaagt ttgaaagatt
    42901 tgaagagata caccaacaaa gagcatatac tgaagatgag atagttgata tgcttgaaaa
    42961 atctggattt aaggatataa aatattatgg ggattttaca tttgacaaac ctagtgaaga
    43021 aagtcaaaga atcttctttg tatgtaaaaa ataagatatt cttgactata taagataatt
    43081 attaaaaata accatgatat aaaaatatat aaaatgcagt tttatagatt aaaagtaaag
    43141 tgatgatatt ttatgatttt tattttctaa taattataaa gttttataga taaaaattaa
    43201 aatataataa ctattgctta gtaaaattaa aataaaatgg gttttgccca aggttaagat
    43261 agataagtat ttatagatta aaattaaggt agtaatataa taaaaataag acaattaacg
    43321 ggacatggag gaatattaat gagagattat gtactaagag cgacatcagg aaatggtcaa
    43381 gttagagcat ttgtcgcaac tactagaaat acagtagaag aagctagaag attacatgaa
    43441 actacaaaag tagcaacagc agcacttgga agaacattaa cagccacatc tataatggga
    43501 ttaatgatga aaaatgatag tgataaattg actgtaataa taaaaggtgg aggtccaatt
    43561 ggaactatta tcgccacatc agactctaag ggaatggtaa aaggatatgt aggaaatcca
    43621 caagtagagg ttgaagacta tccaaatgga aagttaaatg tagcagcagc agtaggtaca
    43681 gaaggtgttg taaaagttat aaaagactta ggtcttagag aaccatataa tgggacttat
    43741 ccattggtaa gtggagaaat tgcagaagac tttacttatt attttgctgt atcagagcag
    43801 actccatcag tagtagcttt aggtgttctt acaaaagaag atgaagtaga gtttgctgga
    43861 ggatttatag ttcaattaat gccagatgca gaagaagaga ctattgctaa gttagaggaa
    43921 aatgtagcta aattaccttc tataactaac atgttaaaag aaggaaaatc accagaggat
    43981 atattaaata ttgttttgga tggtttggaa cctaaaatac ttgatacctg tgaagttggg
    44041 tttatgtgtg aatgttcaaa agaaagagta aaaactgctt tggttgcaat tggtaaaaaa
    44101 tcattggctc aaataataga agaagataaa aaagcagaag ttggttgcca gttctgtaat
    44161 aagaagtata tgtatagtga agaagagttg cttgaaattt taaaggaaat gtagactata
    44221 aaaatagttg tattgatata agtaatgtta tagtaatata gtaaagtcgt attgatatag
    44281 taattaaagt ataatttatt aataatagac atatttatat taaaaaataa gtttaataaa
    44341 tttttaagct taataaattt taagaattaa gaagctttct ggctgtaatg tttgcaatgt
    44401 tgcagttaga aggctttttt attttattat agttcgtgtc aaagattgta tgaaacaata
    44461 aaatattgta aaataatttg tatatttaga cctgattgac acaaaggttt ataagatatt
    44521 tgagttgatt atacgaaatt ctatgaagta gtaaccctgt gactttaatc atgggaggtt
    44581 cagaagaagg agaaaatttt aaatatggaa agtaaaacga agtacatagg aataagtata
    44641 ttaatattat tgatgatatt tttaattgca aagaatttta acggaaaact tgaaatacct
    44701 attcctgaga gtaatagtag aaaatttaaa tcagcagcag tgttttatcc tcaacatcaa
    44761 gatgatgagg ttttatgggg aggaagtgcc attgtagatg ctataaaaca gtgtggtgtt
    44821 gataatgtat atgtggtatt agtatcagat ggttctggag ttaacgtttt caaagctaac
    44881 acaaaattta gaaatttaac taggaagcaa aaggaagaac ttagaaataa cgagtttaaa
    44941 tcagctttga gagagcttgg agtgaaacct caaaatgtga ttatacttgc agatatagat
    45001 aaaaaggaag gcacacatta tgaattaatg gaaaagacta tattggattt tgaacataag
    45061 tttaaaggca atgtgaccca tatagcccat cactataagt atgatgacca tataatgcat
    45121 agaaagaatg gggaagtttt aaagaaactt aaacgtgaag gtaagataaa agatgacttg
    45181 tattttgtta agccaaaata tgtaaagtat ataccaagaa atgaaagagc tatctataaa
    45241 gcaatggatt ttgatgacta caataaggtt aagagtgctt gttatgaata taaggttgta
    45301 aattcaaagc gtaaaatgta tggtatagga tatatttcgg cacatagtta ttttgaacat
    45361 cttctaaatg accctgaact tacgtctgta ttaagtgaag attaatttct tataagtagt
    45421 aagttatgtt tgataagttg attggaattt tctatagttt tgaaggtatt ggaaatttag
    45481 ttttatttaa agatttaaag atagaggttt ttagtgttta aaattttaaa gttaagttga
    45541 ataattgtat agaattattt gtgaaagaag aaacagtgta aaacatagta tcttatttat
    45601 ggttttaaaa tcataggcaa gatacttttt ttatgtaact aataccattt tgagattatt
    45661 aacttaagaa ttttatataa tttaacactc atatataatg atgatattta ggtagaatag
    45721 atgtaattac gcttaaataa aataactgta ttttttataa aaatccaaac cctacactat
    45781 actaccagat aaaatctaca tgctataatt tgacctgtgt ttataaaaaa aggagtaatc
    45841 taataaaata gtatttcact tatgtataac taacaactat tattacgatt ttctctaaat
    45901 aatttaaaga aaaataatgc aggaggtaaa atatgaacaa tgaatttata attttaaaag
    45961 gttgcaaaga gaacaatttg aacaatattt ccttaaaaat accaaaaaga aaaataacca
    46021 tattcacagg ggtgtcgggt tctggaaagt catctattgt atttgagaca attgcaaaag
    46081 aatcacaacg ccaactaaat gaaaggttta gtacatttgt aagaagtttt ttacctaagt
    46141 atggagaggt taaagctgat tgtatagaaa atttgtcaac accaattatc atagaccaaa
    46201 gtagacttgg tggaaattca aggtcaactc taggtactat tacagatgta aattcctttt
    46261 taagagcttt atattcaagg tttggtagtg agtatatagg taatgccaat atgttttctt
    46321 ttaataatat tgatggtatg tgcccacact gtcatgggct tggaaaaaag ctagttccta
    46381 atatgaacca aattcttgat atgaataaat cattaaatga aggtgctata ttgctttcag
    46441 ggtttggagt gggttcttgg cattggaata tattcgctga atcaggattt tttgatatta
    46501 ataagaggat atgtgattat tcagaagtag aattggaaaa gtttttgcat ggtaaagcag
    46561 aaaagattaa gatagaaaat gctggtcaaa ctaacatgac atatgaggga ttaatggtta
    46621 aatttaatcg cctatactta agtagagagg gagaaatatc tgaagctact aagaaaaaat
    46681 tgagtaaact tcttatagaa gatagatgtc ctatatgtga tggaagaaga cttaatgaaa
    46741 gagtatatag gtctttaatt aatggttata atatagcaga tttaactagt atgcaaattg
    46801 atgaattggc tgaagttatt aaaagtatag atgagccaga agcacaacct ttaattaaag
    46861 gaataataga aaagttaaac agtatcatag atataggtct tggatattta acactagata
    46921 gagagacatc ttctctatca ggaggggaat ctcagcgtat aaagatggtt aagtatttaa
    46981 atagtaatct tgttgattta atgtatattt ttgatgaacc aagtgtagga cttcatccaa
    47041 aagatgtgta taaattaaat aatcttttaa aaaaattgcg tgataaaggg aatacaatta
    47101 tagttgttga acatgaccca gatgttatta agattgcaga ccatattgta gatgtaggac
    47161 caaaggcagg taagtatggt ggagaagttg tgtatgaagg cagttatgaa aatctactta
    47221 caagtggtac tttgacaggg aatgctttaa gtaaatttct tcctattaaa gaaagtgtaa
    47281 gagaacatag tggatattta gaagttaaaa attgtaataa aaacaattta aaaaatgtat
    47341 ctattaagat acctaaaggt gtattgactt tagttactgg agtagcagga gcaggtaaaa
    47401 gtacattgat taaggatgaa tttttaaagc aaaatcctag tgctgtactt atagaccaaa
    47461 gccctgtttc agcaaattca aggtcgagtt tggcaacata tagtggaatt atgaacaaca
    47521 taagaagcat attttcaaaa acaaatggcg taaatgcttc tttattcagt tcaaattcag
    47581 aaggagcttg tgataactgt aagggttctg gaatagtaga aatgaatctt gcttttatgg
    47641 agagtataaa gagtacctgc aatatttgtg aaggtaaaaa atttaaaaaa gaagttttag
    47701 aatataggtt tcaagggaag aatataattg aagttcttga aatgtctgtt ttagaggcaa
    47761 ttgagttttt taatttaaaa cagattaaaa cgaaattaca atgtattgaa aaaatgggta
    47821 ttggatacct tactttagga caaactcttg atactttgtc tggaggagag tgtcaaagat
    47881 taaagcttgc tagtgaatta aataaggaaa gttcagtgta tatattagat gaaccaacaa
    47941 caggtcttca tatggctgat attgagaatt ttataagtat aattgaagag attgtagata
    48001 atggaaatac ggtcataata attgaacaca atatagatgt aattaaaaga gctgattgga
    48061 ttattgagct gggacctgaa ggaggaacaa aaggcgggag agtaatattt gaaggtattc
    48121 caaaacaatt atgtaacaat aaaatatcat taacagcaaa atatatagta taataaataa
    48181 aatataataa aattataatg atattttaat aaatataatg ggtatataaa atgataatta
    48241 aacttaacag taataaataa tgtgagaaat attcaaattt aattaaataa agtgtcagaa
    48301 atttgtttaa attttagtat tttaacggtt ctgacctttt ttcttatttt tatggagaat
    48361 atcgaggatg gagtctctaa tagttacttt tttgtgtcta cttgcaagtt tgattaaaaa
    48421 atggctatac acacttgtga cttcagtcat gggaggttca gattataatc ttaatatata
    48481 atttgtgaaa taatttgatg acgatttgct gatgtataaa caataaagat taactacatt
    48541 aaaatttaaa tcttgttaag taaaacttat aaaaggagag agatggatga taaatgatat
    48601 tctaacctta ttatctgaaa gaagtgattt ttttataaag ctatccatag aacatttgat
    48661 gatttctatt atttctataa taattgcgat tgttttgggt ggtataattg gtattattgt
    48721 aagtgaattt cataattctt ctaaacctat attgggaact atcaattttc tatacaccat
    48781 tccatctatt tctatgctag gatttctgat accattttcc ggaattggaa atactactgc
    48841 tgttattgca cttaccattt atgcattatt gccaatggtt agaaatacat atactggaat
    48901 tactaatgtg gataaaaata ttcttgaagc agctagggga atgggaagta cagattttca
    48961 gatattgtat aaaataaaac ttccgttagc cctacctgtt attatgtctg gtattagaaa
    49021 tatggtgatt atgacaattg cattagctgg tatcgcttca tttataggtg ctggaggcct
    49081 aggtgtagcc atatatagag gaatcacgac aaataacaca ataatgacaa ttactggaag
    49141 tatacttatt gctctattag ctttaatatt tgattttatt ttagggctgg tagaaaaatt
    49201 ttttcaacga aaaagaaaaa agaaaaatag gaaaaaagtt tttgccattc taggaagcct
    49261 tattcttata gtaattatct ttacgtctta tttatttaat ccttcaaaga ctcaaactat
    49321 tcatattgct actaaaccta tgactgaaca atatattctt ggtgagatgt tggatatttt
    49381 aattgaaaaa aacacaggtc taaacgtgga aattacacaa ggtgtaggag ggggaacatc
    49441 taatatccag ccagctatgg agaatggaga atttgacatt tatccagagt atacaggtac
    49501 aggctggaat atggtactta agaaaaatga attatatact gaaagtatgt ttagtaaact
    49561 acagtcggaa tacaaaacta aacttaaaat gcaatggact ggtatgtatg gtttttctaa
    49621 tagctatgca ttagcagtta gaaaagaagt tgcagataaa tacaatttaa aaaatataac
    49681 ggacttaaaa gatgtatctt ctaaattgac atttggagct gaatatgact tttatgagag
    49741 agaggatgga tatactccat tgtgtaatac atatggactg aactttaaag atacagttga
    49801 tttagatatt ggattaaaat accaagctat caatgaaggt aagattgacg taatgcctat
    49861 atttacaact gatggtcagt atagtgtttc agatgttgta gttctaaaag atgataaaaa
    49921 tttctaccca tcttatcaat gtggaaatgt tgttcgtttg gaagtattag aggaaaatcc
    49981 agaactatca aaggtattta agatggttgg agatattttg actgataaag aaatggctaa
    50041 aatgaattat gacgtagaaa acaacaattt agaacctaga gaagttgctc gtgagtttct
    50101 gaataagaaa ggtcttttat aaattggagg aaatataatg acacctatta tacaatttaa
    50161 aaatattaaa aaacaatata atgataaaac tataatagat aatcttaatt tagatataga
    50221 aaaaggagag tttttgactg taattggttc gtctggttct ggtaagacaa cactcttaaa
    50281 aatgattaat ggtctgattt tgccagatgg aggaaatatt ttgataaacc aaacagatat
    50341 caaaaacgag gacttaatta aattaagaag aagaatagga tattgtgtac agggaagtgt
    50401 tctttttcct catatgactg ttgaagaaaa tatttcatat gtaccaaatc tcctaagtaa
    50461 aaagaataaa ttagaagtaa aaagtgctgt aaacaagtgg atggaaatag tgggattgcc
    50521 aaatgatatg aaagtgcgtt acccatcaga attgtcaggt ggtcagcaac aacgtgtagg
    50581 gattgcaaga gctttagctt cttctccaga aattttatta atggatgaac cttttggtgc
    50641 agttgatgag attacacgaa agcaattaca aaaagagata aaagaaattc ataaaaaaac
    50701 aggagttacg attatcttta ttacgcatga tatttatgaa gctttgattt tgggaacaaa
    50761 gactttggta ttaaatcatg gagttgttca acagtatgat actcctgaga atattttaaa
    50821 tactcctgct aaccaatttg tagaccagtt attgaatata agaaaatcta taatagatga
    50881 tgataattta aaagaataaa tttaaattta aaatcaaatt gttgtgataa aaactattga
    50941 tttagagcta acttcaagtt gtatacttga agtggaaaag ttagattaag gtatagaagg
    51001 gcatttagca gttggctgta gacgaggtaa ctgctgaagg tgctttcaat aaatggctag
    51061 aacctgtatc tgttgaagat tataataaat tgaaataaga agctcaatat acagagtaga
    51121 taggcacttc aaatagaagt gtcttttgtg tgccataaaa taaatcaaaa tattagcaaa
    51181 gaatgtgaaa aacaaaataa aataatatgc tatctttaaa ataggaggga tgctatatgg
    51241 ataacaattt gattaccatt gaaaaaactt tagattatat agaaaaacat cttttagatg
    51301 agatgttaga tttaaatgta atttctaaag agataggcta ttcaaaatac catttacata
    51361 gaatgtttac atcagttgtc ggatttagtg tacataatta tatacaaaga cgtagactta
    51421 ctgaagcagc gagaatgcta atttttacag aaaaaccaat tattgagatt gctttaatct
    51481 cggggtatga tacacaacgc tctttttcaa gagggtttaa aaaaatgttt aaacgtagtc
    51541 ctaatttttt tagaagacat caagattttt taccactaca attaaaattt gatattcata
    51601 atagaaaaaa attacgtgga gatatggttt tagatgttaa aaaagttaat tgtgcagata
    51661 taaatattgt tggctattct ggtaaagtaa gaaaaggatt tcatattatt ggaaaatgct
    51721 ggagggattt gcataaaaat aaagagatga tagaaaataa ggtagattta ggttttttaa
    51781 ttggagttag tgattatagc aattttaaag agattgagaa tactcctaca tttacataca
    51841 ttgcaggtgc tcaagtagct acttttggca aattacctaa tggaatggaa aaattcgtat
    51901 taccgaaaag taattatatt gtttttactt ttattgggaa aaatgaggat agtatgcaaa
    51961 atgttgtaga atatatctat caagaatggt tccctaattc aagttgccag tttaatgaaa
    52021 ataatttata tgactttgta aaatatggag aaaaaactga tgagaatggt ttgagtgaaa
    52081 ttcagttttg ggttccaatc atttaaaaga aggtattatg tataaagtag atggatttat
    52141 ataggcaaag ttttacagca gtatgtattt ataaataatt tatagtttgt aggaaagaaa
    52201 taattttact ataagattat gataaaatac acaatactat gacaaaatag tgttaaataa
    52261 tagatttaat tttgcatagg aaaagttcta taatggtgtg tattcataaa taatttataa
    52321 tctgtaggaa agaaatagtt ttactataag attatgataa aatacacaat actatgataa
    52381 aatagtacta aataatagat ttaattttgc aaaggataag gtgttttaat aaaaatgaat
    52441 gtagaaaacg aattacttaa aaacctagat agattgcata caacagagtt aggcgttgta
    52501 agaattaaga aaaatctttc tttggagaca aatgatgtag tgaactggtg taaaactaaa
    52561 atagaatctc caaatgccat tattaataga aagggaaaaa actggtatat aagtgtatat
    52621 gattgtatta taacagtaaa cgctcacagt tatacaatta ttacagctca taaagaaaag
    52681 aaatgaattg caaaatacaa aggtcaaaag cattttccac tgtttgtagt acctatgaag
    52741 aagtatataa aataatatta cttttgaata ataatttaaa ttttggaggt tgaaaaaatg
    52801 aatgatactg taaaagaaaa attggcagag tcactaactt ctgatactac tgatatttta
    52861 ccttatttgc cttatttatt acaagatttt tgggaattag gaagttcacc agaacaaatg
    52921 ttacaattat ttagaaagca tatacctttt aatgaggata caaaagtttt ggatttagct
    52981 tgtggaaaag gtgctgtatc tgtgaagctt gcaaaagagt tgaatataca tatcaaaggt
    53041 attgatttga tacctgaatt tattaattat gctagaagaa aggctattga atataaggtt
    53101 gatgctttgt gtgattttga aatgaatgat gctaatagtt ctgtaaaaat tgagcatgat
    53161 tatgattgtg tgatttttgg agctgtaggg gatattttag gtaattataa agaaactata
    53221 agtaagctat tacctacaat aaaaagtaag gggttcattt taattgatga tgcatatgta
    53281 gtagataaat ctacaaatga acagcttaat tttgagaggg tgtatcccac ttacaatgag
    53341 tggtgtatat tgttcaaaga attaggtctg aagctaattg aatgtatacc atgtttagaa
    53401 cttgaaaatg atgttgaaga ggaaatgaat tggattataa ctcgtgcaaa tgagctaatt
    53461 aaaaaatatc cttctcaaaa agatatgttt gaacaatatg ttattagcca aaaaaatgaa
    53521 tatactgatt tacagcagga ccttgttgga gcaacttgga ttttacaaaa agaataagat
    53581 aaatattaga ctagataatc aatgttatag tgaattatcc catgtctagt gaatataaat
    53641 aacataaaaa accgtcaaga tgaatagttt attcgtgctt gacggttttt agtatttgtt
    53701 gaagtaaaat tttatttctt tagtatatca cttttagtca atccatttga tttagcttga
    53761 gtattccatt gatgattccc cataagttta tataggaatc tagggaagtt aggtgaaata
    53821 aaaatagtac ttttgttgga atcatctata tgtatattat cactaagtat attttttgaa
    53881 agttctttta atgcaattcc taaatctttt ttaggtcctt tacccaaagg tacggattct
    53941 agtgaaccaa acatctcccc agctccaatt ccaatacctt gaacccatgt aaagttgatt
    54001 tttttacacc aatgctctat catttgaatt gccaaatgat tatgttcacc ttcattgaat
    54061 ccacagttaa ctattgcata cactggaata ggttttttat tttgtgtttt taaaaatttt
    54121 tcacatttct ctaaaaaatg aagtaaatga gatggaatac catctacata aagaggagat
    54181 gcgattatta atctatcaca attaagtatg gcattaagag cgtcaggata cttagatgtg
    54241 ctatctactt taaatgtaaa aactttatta tcattttgaa taagttgttt taattcagat
    54301 aaaaaatact gtgaattatt tttatttact tttggactac catttatcat tactatattc
    54361 atatggattc acctcttata tcttgtatat tttcatagaa acaaacagag tgttctttta
    54421 tattgaaatt tatagcattt ccatttacta atttcgtagc agtttctttt tcagcttctg
    54481 taatacaatc tccatagaat atagctttta aatttatctg tttatcatat ctgcttttat
    54541 gacgcatcat attatctttt attacaaagt atggaagaat ataattaatg cttttatcaa
    54601 gtacgttttt gacaaaaggg ctaaaccctc cgtaacagca tctgcttatt ataataattc
    54661 catcagagtc atttataagt ttacccatgt tgctgtaata atcttttaca acacattcag
    54721 aaggagtttt aatccagcaa ccaaaacaac caatacattt gtggatttct tttgtgtttg
    54781 gaagtatttt tatattgtca tcaagtttgg gtaataactt ttctaattct ttgtcagtta
    54841 aatcatgtat taaaagcctc ataaagtcca tctcctttta ttatgtatgt ttacttctaa
    54901 aaattatatc ttatagattt ttcagtaaat ctttacacca attaataaca aaattataat
    54961 agtcaactcc ataaatcatt gttgcataca tatatgtata ttggtcaacg caatgatttt
    55021 cttctgcatc atttttttgt ttacttaatt tatttagaag taattctact tcagttatta
    55081 acatttttaa gtttgtttca attatttctt tgggtagata cttgtaaaag aatatattaa
    55141 caaggtggtc ttgctttgtt ttagaaaaat ttataggttt ctttaaccat tcaagaaata
    55201 cttctttacc agtattagtt atggaatata atttttttaa tttgctacca tctataactt
    55261 cgtgatagtg tatatatcct tttgtttcta atcttttaag tgcaggatag atacttccaa
    55321 agctggcgtc aaaaaagtac gaggtacttt ctgacattat ttgttttaag tcgtatccac
    55381 ttaattcttt ttccattaaa aatcctaaaa taatgtattc taacatatta cctcctggta
    55441 tcaattagat atatatcgat tcgatatatt tattattaca gataataaat cttacgtcaa
    55501 gatgcttttt aagataagct aaaaagaatt aggagttatt gataaatatg caaaaaaatt
    55561 aatataaaag gtaattaaat atcaatgaag atttaaaaat agttaaatca aagtaagagt
    55621 ctaatatata aaatttatag taactttatg gtttaaatat aaacttgcgt gctattgaca
    55681 aagtataaaa ttagcagtaa aataattgca tgagcaaata gattattgcg aacgcaaaaa
    55741 aataaggaca atagatatta aaggagagaa tatgagagag ctattaaaat gggtttccat
    55801 tataaataga tacaatacaa attatattga caggaaattg gcggatattg gaatcaataa
    55861 tagccaatat ttttatgtat tacacatatg taataatcca ggtattacac aggacactat
    55921 atttgagaaa attcttgtga atcctagtaa tataacaaga gctttggcaa atttagaaaa
    55981 agaaggcttt ataacaagag aaccaggtgt aaaggataaa agaacatggc atttatatcc
    56041 tacagaaaaa tctttaggat gttatgatga aatactaaaa ataacaaatg ggtatgttga
    56101 ggagttgttg agttcatttg agaaagaaga gcaagagttg tttatgtcaa tgttaaagaa
    56161 agttgcattt ttggctattg atatgaatga agaagcaaaa ttggaggcga aggaaaatgg
    56221 aagaaaaata taaggagcca ttggaatatg aaaaggtttc aaagttatta actaagtacg
    56281 caattcctag tattatagca atgttagtaa gtgctttata taacattgtt gaccaaatat
    56341 ttattgggca aggtgttggt gtattgggta atgctgctac aaatgtcact tttccactaa
    56401 caacaatatg tactgcaatt gcattattgt taggaattgg aggagcgtct aagtgttcat
    56461 tggaattagg tgctaaaaat tcagaaaaag ctacaaaagc agcaggaaat tctatatgct
    56521 tgatggctgt ttttggaata agtttattta tagtagtatc aatattttta acaccactat
    56581 taaaattctt tggctctaca tcagaaatac taccttatgc acacacatat acaaaaatta
    56641 catcaattgg attaccattt cttataatgt ccactggaat gagtaagttg atacttgcag
    56701 atgggagacc aaaagcttct atgttttgta tgttaatagg agcagttatt aatacaattt
    56761 tgaatccatt attcattttt gtttttaata tgggaattgc tgggtctgct ttagcaactg
    56821 ttattgggca acttatatct tttggaataa gtgttcatta tataaagaat tttcaacata
    56881 ttaaattaca taagaaaagt tttaaattag atagtataat ttataaacat atatttgcgc
    56941 taggttcaag ctcatgtttt aatcaattag caatgactgt aacacagata gttatgaata
    57001 atacattggc atattatggg gctaggtcag tttatggaag tgagattcca cttgcttgtg
    57061 tcggtatcat tattaaagta aatatgattt ttatatctat aattattggt atatcacaag
    57121 gtatgcaacc aattataggg tataattatg gggctgaaaa gtataatcgt gtaaaagaag
    57181 catataagtt tgcagtggga agtgcaacta ttatatctgt tattgcattc ttatgtttcc
    57241 aattcttccc aagacaaatt acgtctatat ttggtaacgg aaatgagact tattttatat
    57301 ttgcagaaca ttattttaga actttcttgt tcttaacatt tataaatggt gttcaaatag
    57361 tatcatcaac tttctttaca tcaattggga aatcagtgtt gggacttttt acttctttga
    57421 ctcgtcaaat tttattttta attccattga tagtaatttt accaatgttt atgggaattg
    57481 atggagtaat gtttgcagga ccaatagcag atgcagcagc ggcaatggta tgtatatact
    57541 ttgcagttca tgaaatgaaa tcattgacta taaagcaaaa taatttgatg agtaaccata
    57601 ataagattgc agaggtataa gacaatattt aagaagtata gaaaattaat ttaaaaaata
    57661 taataaatta atttaggaag tgtagaaaat taatctaaag aagtataata aactaattta
    57721 ggaagtatag aaaattaatc taaagaagtg taataaacta atttaggaag tatagaaaat
    57781 taatttaaag aagtataata aactaattta agaaatatag aaaattaatt taaagaaata
    57841 caatacatta atttttaagt aacattaaat taatgtaagt aaaataatgt tcatatgaat
    57901 atttaaaggt gcattttctg aagctaagta tatgttgtag agtgttatgt ctctctatcc
    57961 tgtgaagttt aatagctcaa gaaaatgcac tatttatgat gattattttg ttttatttcc
    58021 aattttggaa ataactattc taattaaatc taaaattata ccataaataa gaaaagaaag
    58081 tatagatgct atgtaccaat aaatcgaaat tgaccctgta attgttctac atgggatttg
    58141 catacctgaa tccataaaaa caaaaaaact atcaactctg gaaataatag acaaaatagg
    58201 atttaaacct attagaacaa tactttttga gtcttgacca atttgatgca tataaatctc
    58261 aaataatgca attaaaaaaa accaagatgt aaaagattta ataatttttt tcatcaaaat
    58321 acctcacata atataaattc tgtttgcact atttgcttac tacaactatt tatctccatt
    58381 cagtcacatc acttaattgt aatctaggtc ttcttgatgg ttcttcatca ggataaccta
    58441 aagatattgc tgccactaat tgactatctg tatttaacca ttcacataat tcacgatatg
    58501 caaagtatac atcacaaatc caaagactac ctagacctaa ttccacagct gtaagcgaca
    58561 tattctgaat tgcagctcca attgattgca tatttgccat ttcatagaat cgttcttcag
    58621 gggatagctt ttctaaaggt gatttaccta atatatttaa aataaaaatt gttactggag
    58681 cttgtttcat gatttcaact gtgtaatttg caccagcgat atgttgacga cttcctggaa
    58741 gaagcccatt atcattaatt tcattttgaa tacctttgct catggcttta agcatagatt
    58801 ctttttcctt ttcagtgatg acaacaaaac gccatggttg cctgttttta gatgaaggtg
    58861 catttattcc agcttcaatt attttctcta tagtttcatg tgaaatgctt tgatttttat
    58921 attttctgat acttcgtctt tttgatatag aatcacttat cattttaagt aacctccgtt
    58981 tattttttgg aaaaatctat ttaattttta ttttctattt taattatttt agcattaatc
    59041 agaaaaaaag tcattgattt tagtatacaa ttattaatga ttatattaag aattgtatca
    59101 aaaagtatgt gattatacta agggattata ttaaacatta tatttaaaat tgtatgaaag
    59161 gt
//
LOCUS       DAMJYX010000015        55762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJYX010000015 DAMJYX010000000
VERSION     DAMJYX010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.015"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            7..81
                     /locus_tag="PC479_000992"
     tRNA            7..81
                     /locus_tag="PC479_000992"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            87..172
                     /locus_tag="PC479_000993"
     tRNA            87..172
                     /locus_tag="PC479_000993"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Leu,seq:taa)
     gene            188..263
                     /locus_tag="PC479_000994"
     tRNA            188..263
                     /locus_tag="PC479_000994"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Met,seq:cat)
     gene            271..345
                     /locus_tag="PC479_000995"
     tRNA            271..345
                     /locus_tag="PC479_000995"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:304..306,aa:Glu,seq:ttc)
     gene            360..433
                     /locus_tag="PC479_000996"
     tRNA            360..433
                     /locus_tag="PC479_000996"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:392..394,aa:Gly,seq:tcc)
     gene            439..514
                     /locus_tag="PC479_000997"
     tRNA            439..514
                     /locus_tag="PC479_000997"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:472..474,aa:Val,seq:tac)
     gene            520..596
                     /locus_tag="PC479_000998"
     tRNA            520..596
                     /locus_tag="PC479_000998"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:554..556,aa:Asp,seq:gtc)
     gene            607..681
                     /locus_tag="PC479_000999"
     tRNA            607..681
                     /locus_tag="PC479_000999"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:639..641,aa:Gly,seq:gcc)
     gene            691..764
                     /locus_tag="PC479_001000"
     tRNA            691..764
                     /locus_tag="PC479_001000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:725..727,aa:Arg,seq:tct)
     gene            1748..2587
                     /locus_tag="PC479_001001"
     CDS             1748..2587
                     /locus_tag="PC479_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2803571.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2584..3765
                     /locus_tag="PC479_001002"
     CDS             2584..3765
                     /locus_tag="PC479_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803572.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4069..4971
                     /gene="ptb"
                     /locus_tag="PC479_001003"
     CDS             4069..4971
                     /gene="ptb"
                     /locus_tag="PC479_001003"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2803573.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            4993..6072
                     /gene="buk"
                     /locus_tag="PC479_001004"
     CDS             4993..6072
                     /gene="buk"
                     /locus_tag="PC479_001004"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2803574.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6062..6739
                     /locus_tag="PC479_001005"
     CDS             6062..6739
                     /locus_tag="PC479_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803575.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6782..6997
                     /locus_tag="PC479_001006"
     CDS             6782..6997
                     /locus_tag="PC479_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2803576.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7026..8105
                     /locus_tag="PC479_001007"
     CDS             7026..8105
                     /locus_tag="PC479_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2803577.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8105..8857
                     /locus_tag="PC479_001008"
     CDS             8105..8857
                     /locus_tag="PC479_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2803578.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8858..9415
                     /locus_tag="PC479_001009"
     CDS             8858..9415
                     /locus_tag="PC479_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2803579.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9585..10931
                     /locus_tag="PC479_001010"
     CDS             9585..10931
                     /locus_tag="PC479_001010"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2803580.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11198..13030
                     /gene="glmS"
                     /locus_tag="PC479_001011"
     CDS             11198..13030
                     /gene="glmS"
                     /locus_tag="PC479_001011"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2803581.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13481..14776
                     /locus_tag="PC479_001012"
     CDS             13481..14776
                     /locus_tag="PC479_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2803582.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15232..15969
                     /locus_tag="PC479_001013"
     CDS             15232..15969
                     /locus_tag="PC479_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2803583.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            15990..17243
                     /gene="murA"
                     /locus_tag="PC479_001014"
     CDS             15990..17243
                     /gene="murA"
                     /locus_tag="PC479_001014"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2803584.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17330..18394
                     /gene="spoIID"
                     /locus_tag="PC479_001015"
     CDS             17330..18394
                     /gene="spoIID"
                     /locus_tag="PC479_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2803585.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18460..19128
                     /locus_tag="PC479_001016"
     CDS             18460..19128
                     /locus_tag="PC479_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2803586.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19226..19486
                     /gene="spoIIID"
                     /locus_tag="PC479_001017"
     CDS             19226..19486
                     /gene="spoIIID"
                     /locus_tag="PC479_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2803587.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19683..20702
                     /locus_tag="PC479_001018"
     CDS             19683..20702
                     /locus_tag="PC479_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2803588.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20718..21146
                     /gene="fabZ"
                     /locus_tag="PC479_001019"
     CDS             20718..21146
                     /gene="fabZ"
                     /locus_tag="PC479_001019"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2803589.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21257..21766)
                     /gene="yyaC"
                     /locus_tag="PC479_001020"
     CDS             complement(21257..21766)
                     /gene="yyaC"
                     /locus_tag="PC479_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2803590.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      21955..22058
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22195..23388
                     /locus_tag="PC479_001021"
     CDS             22195..23388
                     /locus_tag="PC479_001021"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2803591.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23703..24800
                     /locus_tag="PC479_001022"
     CDS             23703..24800
                     /locus_tag="PC479_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803592.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25064..27283
                     /locus_tag="PC479_001023"
     CDS             25064..27283
                     /locus_tag="PC479_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2803593.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27286..28068
                     /locus_tag="PC479_001024"
     CDS             27286..28068
                     /locus_tag="PC479_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2803594.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28400..30313
                     /locus_tag="PC479_001025"
     CDS             28400..30313
                     /locus_tag="PC479_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2803595.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30454..30762
                     /locus_tag="PC479_001026"
     CDS             30454..30762
                     /locus_tag="PC479_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2803596.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30786..31112
                     /locus_tag="PC479_001027"
     CDS             30786..31112
                     /locus_tag="PC479_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803597.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31151..32485
                     /gene="celB"
                     /locus_tag="PC479_001028"
     CDS             31151..32485
                     /gene="celB"
                     /locus_tag="PC479_001028"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2803598.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            32496..33017
                     /locus_tag="PC479_001029"
     CDS             32496..33017
                     /locus_tag="PC479_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2803599.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            33110..34069
                     /locus_tag="PC479_001030"
     CDS             33110..34069
                     /locus_tag="PC479_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2803600.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34118..35170
                     /locus_tag="PC479_001031"
     CDS             34118..35170
                     /locus_tag="PC479_001031"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2803601.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35173..35847
                     /locus_tag="PC479_001032"
     CDS             35173..35847
                     /locus_tag="PC479_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2803602.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36400..36951
                     /gene="raiA"
                     /locus_tag="PC479_001033"
     CDS             36400..36951
                     /gene="raiA"
                     /locus_tag="PC479_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2803603.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37083..39758
                     /gene="secA"
                     /locus_tag="PC479_001034"
     CDS             37083..39758
                     /gene="secA"
                     /locus_tag="PC479_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2803604.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39773..40880
                     /gene="prfB"
                     /locus_tag="PC479_001035"
     CDS             join(39773..39847,39849..40880)
                     /gene="prfB"
                     /locus_tag="PC479_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2803605.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41024..43165
                     /locus_tag="PC479_001036"
     CDS             41024..43165
                     /locus_tag="PC479_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2803606.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43313..44479
                     /locus_tag="PC479_001037"
     CDS             43313..44479
                     /locus_tag="PC479_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2803607.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44539..44656
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44781..45386
                     /locus_tag="PC479_001038"
     CDS             44781..45386
                     /locus_tag="PC479_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2803608.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            45715..46755
                     /locus_tag="PC479_001039"
     CDS             45715..46755
                     /locus_tag="PC479_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2803609.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            46894..47346
                     /gene="tsaE"
                     /locus_tag="PC479_001040"
     CDS             46894..47346
                     /gene="tsaE"
                     /locus_tag="PC479_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2803610.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            47346..48062
                     /gene="tsaB"
                     /locus_tag="PC479_001041"
     CDS             47346..48062
                     /gene="tsaB"
                     /locus_tag="PC479_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2803611.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            48052..48528
                     /gene="rimI"
                     /locus_tag="PC479_001042"
     CDS             48052..48528
                     /gene="rimI"
                     /locus_tag="PC479_001042"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2803612.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48534..49550
                     /gene="tsaD"
                     /locus_tag="PC479_001043"
     CDS             48534..49550
                     /gene="tsaD"
                     /locus_tag="PC479_001043"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2803613.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            49847..52555
                     /gene="hpdB"
                     /locus_tag="PC479_001044"
     CDS             49847..52555
                     /gene="hpdB"
                     /locus_tag="PC479_001044"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2803614.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52559..52816
                     /gene="hpdC"
                     /locus_tag="PC479_001045"
     CDS             52559..52816
                     /gene="hpdC"
                     /locus_tag="PC479_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2803615.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            52809..53759
                     /gene="hpdA"
                     /locus_tag="PC479_001046"
     CDS             52809..53759
                     /gene="hpdA"
                     /locus_tag="PC479_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2803616.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            54075..55037
                     /locus_tag="PC479_001047"
     CDS             54075..55037
                     /locus_tag="PC479_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2803617.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(55136..>55762)
                     /locus_tag="PC479_001048"
     CDS             complement(55136..>55762)
                     /locus_tag="PC479_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2803618.1"
                     /translation="ILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPT
                     SFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGV
                     IILGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAII
                     HVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        22355 a         6020 c        10591 g        16796 t
ORIGIN      
        1 aaatgttcca aggtagctca acggtggagc aaccggctgt taaccggtag gctgtgggtt
       61 cgagccccac ccttggagcc aaaaaagccg aagtggcgga actggcagac gcacaggact
      121 taaaatcctg cgggacttac ctctcgtacc ggttcgattc cggtcttcgg cacttataaa
      181 acaatatcgc ggggtggagc agttggcagc tcgtcgggct cataacccga aggtcgtagg
      241 ttcaagtcct gcctccgcaa ccattaaaac ggctcgttgg tcaagaggtt aagacaccgc
      301 cctttcacgg cggtaacagg ggttcaattc ccctacgagt caccaattca attaaatatg
      361 cgggtgtagc tcaatggtag agttctggcc ttccaagcca gctgtgaggg ttcgatcccc
      421 ttcacccgct ccaaaaatgg gaccatagct cagctgggag agcacctgcc ttacaagcag
      481 ggggtcacag gttcgagccc tgttggtccc accattcgcg gcctggtagt tcagttggtt
      541 agaatgccag cctgtcacgc tggaggtcga gggttcgagc cccttccagg tcgccaaatt
      601 aaataagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa gtcgcgagtt
      661 cgaatctcgt ttcccgctcc ataataactt gggtgcatag ctcagctgga tagagcaacg
      721 cccttctaag gcgtgtgtcc ggggttcgaa tccctgtgcg ctcattaaga aaagtttact
      781 ataaattata gtaaactttt atattgttta aaaagacaca ggaaacatgt atattacctg
      841 tgtcttttta tttataaact ttaaactatg taaaaattac agtatattct aatagagtta
      901 cagtatattc attaatattg taaacatacg gagggtaatg tataatagtc tacaagatgt
      961 ttatgaaggc atcagttaaa tataataaat ataactaatt agtaagagtt atatacatta
     1021 tattgtatac tttatggtat ttatatattg aaataactta tgaaattatt tataaagaga
     1081 ttcatgagca tatttttgtc taaacaataa gtataagaaa ttataaaaaa ttagagacta
     1141 tagtgaattt acaatttaat gtaatattta taagttatac taaataaatt attatactgt
     1201 gaaatggata tataacttta ggtattgtgt attattttca ctgtaggaga ttatatctag
     1261 ttatacataa taagttttaa tgatagttta tagtaagata ttaaaataaa tttatctaat
     1321 ctagatatga aataagttat tactttaata taaaaatgaa tctaatggaa taatattgat
     1381 gataagtaat atttagattt tgtatttgtt tatagttaat aagtggatta tagaatttat
     1441 aataattaat atatattaaa caaagatata ttaagatatt ttagaacata aaactgaatt
     1501 aaggcgcgag tactattttg tattcgcgcc ttgatggttt gagttatttg agtaaaaact
     1561 aactttaata agaaatatgt atttattaag ttgatatttt taatgtgttt agagattatt
     1621 taacaatctc caaatataaa actagaatta tttacataaa agttaaatag tttgtataga
     1681 gttagattta tatagtataa tacttagtgt aaataattaa gaagttatca aacaggagga
     1741 taaaactgtg ttagatataa attctttttt aaatggattt tttaatataa tattaagaat
     1801 gagtatatat gatttaattg acatatctat agtagcgtat attttttaca aaatatttat
     1861 gtttataaaa gatacaagag cagaacaagt gtttaaaggt ataatatttc ttttattagc
     1921 aactcaactt agtaacacct ttaaattgca taccgtttat tggatatctc ttaaagcttt
     1981 agattatgga gttatagctg ctcttataat ttttcaacca gaatttagag caggacttga
     2041 acatatagga agagcaaagt ttaatttatt tggaaagaat gtaaatactt ctgaagaaac
     2101 tctcaataga aatatagaag aaattgtaga agctttatat tcactttcta gacaaaaaat
     2161 aggagctctt atcataatgg agagagaaac tagaataagt gatataataa atacaggaac
     2221 tattattgat gctgaaattt ctaggcaact attaataaat atatttatac caaatacacc
     2281 attacacgat ggagctgttg taattagaga ttctaaggta aaagcagctg catgtttttt
     2341 gccattgaca gaaagtaagg atttaagtaa agatttgggt actagacata gagcaggaat
     2401 aggggtaagt gaggtgtctg actgtataac tttgattgta tcagaggaaa caggtggcgt
     2461 atcaattgct aaagcaggta agttatatag agatatttct agagaaagaa tgatgaatat
     2521 attacgtagt aatttaaaaa caaatacaga aacaagaagt tttttcaaag gtggtatatt
     2581 taaatgataa atagactaaa aaataatacc aaaataaaaa taatatcatt tttaagtgca
     2641 attgcattat ggatgtatgt tatggctgta gttgaccctg aagaaactaa agggtttgaa
     2701 gatatacctg tatctataag taatatgagt gatttaaagg ataaaaatct tgttatatat
     2761 ccagacgaaa aattaacagc agatgtatat ataaaaggta gtttatcaaa ggtacaaagt
     2821 gttaaaaaag aagatataca tgtatatgga accattgaag acccaataca aggacagaag
     2881 gaagtatatt taaaagcaaa tattcctcaa ggtgttactc tagaatttaa gcatgatgtt
     2941 ttagtagtaa atcttgaaaa aaacataaaa gaaaaaagag atataaaggt agaagtaagt
     3001 ggaaattcaa aaagaaatat aaataaaatt gaacttagta aggaatttgt cgatgtatca
     3061 ggtccaagaa ctctagttaa agaagtcaaa agtgtaagag ctaatctaga tgttggtaat
     3121 gagacagatg atttttctaa aaaattgcaa ttaataccat taaatagtga tggaactaag
     3181 gttgaaggag taacattaag ttcatccttt gtgactgcaa agatagaact attgaaggaa
     3241 aaaattgtcc caataagagt aagtctaaat gataataaag ataatgatgt actgaaaaac
     3301 tataaattaa gtaaggaaca agttacaata aaaggaaaga aagaagatat agacaaaata
     3361 agttatatta agacaaaacc tattgactta gctgatttgg ctaatggtga agaaaaagaa
     3421 gttagcttag aaattccaga gggtataact cttgaggata gctctataac aattaaatta
     3481 aatgatacaa aacaattgag tgcagaattt ttatataatg cgggagatat tgaattaaga
     3541 aatatcccaa caaatatgga taatccaaat gtaatttctg atgtagatat aaaagttgtt
     3601 gtagagtcaa tggaggattt gagtaagtta aataaaaaag atatttcatt atatattgat
     3661 ttatcacaag gtcctgagga agatagtaaa tataaaataa aatatgaaac aacatatcaa
     3721 tttaaaaaaa taaatataga acctaatatt gttgaagtgg aatgaacaaa tatttcgaat
     3781 atttagttgt tacaagatgc gacaaaaaaa cctataaaat taagtttgtt aattttatgt
     3841 ttttgttgca tcttgtttat tttagttata ttataaaata aggacaatga gatatttgtt
     3901 aaatctaaaa agcgctagat atatgaaaaa agactgataa atagatttaa ataaatattt
     3961 tgaattaaat atttcaatta tctctaggaa aacgttgctg aacgagttat aatttaattg
     4021 agatataaaa agttctattt caaaaaattc aagatgaggt ggaaatgcat gagaagtttt
     4081 gaagaagtaa ttaagtttgc aaaagaaaga ggacctaaaa ctatatcagt agcatgttgc
     4141 caagataaag aagttttaat ggcagttgaa atggctagaa aagaaaaaat agcaaatgcc
     4201 attttagtag gagatataga aaagactaaa gaaattgcaa aaagcataga catggatatc
     4261 gaaaattatg aactgataga tataaaagat ttagcagaag catctctaaa atctgttgaa
     4321 ttagtttcac aaggaaaagc cgacatggta atgaaaggct tagtagacac atcaataata
     4381 ctaaaagcag ttttaaataa agaagtaggt cttagaactg gaaatgtatt aagtcacgta
     4441 gcagtatttg atgtagaggg atatgataga ttatttttcg taactgacgc agctatgaac
     4501 ttagctcctg atacaaatac taaaaagcaa atcatagaaa atgcttgcac agtagcacat
     4561 tcattagata taagtgaacc aaaagttgct gcaatatgcg caaaagaaaa agtaaatcca
     4621 aaaatgaaag atacagttga agctaaagaa ctagaagaaa tgtatgaaag aggagaaatc
     4681 aaaggttgta tggttggtgg accttttgca attgataatg cagtatcttt agaagcagct
     4741 aaacataaag gtataaatca tcctgtagca ggaagagctg atatattatt agccccagat
     4801 attgaaggtg gtaacatatt atataaagct ttggtattct tctcaaaatc aaaaaatgca
     4861 ggagttatag ttggggctaa agcaccaata atattaactt ctagagcaga cagtgaagaa
     4921 actaaactaa actcaatagc tttaggtgtt ttaatggcag caaaggcata attttaaggg
     4981 ggaaataggc aaatgagcaa aatatttaaa atcttaacaa taaatcctgg ttcgacatca
     5041 actaaaatag ctgtatttga taatgaggat ttagtatttg aaaaaacttt aagacattct
     5101 tcagaagaaa taggaaaata tgagaaggtg tctgaccaat ttgaatttcg taaacaagta
     5161 atagaagaag ctctaaaaga aggtggagta aaaacatctg aattagatgc tgtagtaggt
     5221 agaggaggac ttcttaaacc tataaaaggt ggtacttatt cagtaagtgc tgctatgatt
     5281 gaagatttaa aagtgggagt tttaggagaa cacgcttcaa acctaggtgg aataatagca
     5341 aaacaaatag gtgaagaagt aaatgttcct tcatacatag tagaccctgt tgttgtagat
     5401 gaattagaag atgttgctag aatttctggt atgcctgaaa taagtagagc aagtgtagta
     5461 catgctttaa atcaaaaggc aatagcaaga agatatgcta gagaaataaa caagaaatat
     5521 gaagatataa atcttatagt tgcacatatg ggtggaggag tttctgttgg agctcataaa
     5581 aatggtaaaa tagtagatgt tgcaaacgca ttagatggag aaggaccttt ctctccagaa
     5641 agaagtggtg gactaccagt aggtgcatta gtaaaaatgt gctttagtgg aaaatatact
     5701 caagatgaaa ttaaaaagaa aataaaaggt aatggcggat tagttgcata cttaaacact
     5761 aatgatgcta gagaagttga agaaagaatt gaagctggtg atgaaaaagc taaattagca
     5821 tatgaagcta tggcatatca aatctctaaa gaaataggag ctagtgctgc agttcttaag
     5881 ggagatgtaa aagcaatatt attaactggt ggaatcgcat attcaaaaat gtttacagaa
     5941 atgattgcag atagagttaa atttatagca gatgtaaaag tttatccagg tgaagatgaa
     6001 atgattgcat tagctcaagg tggacttaga gttttaactg gtgaagaaga ggctcaagtt
     6061 tatgataact aatgataaaa gaataagaat cataactggt cactatggaa gtggtaaaag
     6121 tgaatttgcg atgaactatg ttgttaagtt aagagatatg gtttctggca aagttgctat
     6181 agccgattta gatgtggtaa atgtatactt tagaactaga gaaaagaaag agttaatgaa
     6241 atccttaggt atacaaccaa tagatagctc aattaatgct ccaacattag atttaccagc
     6301 agtatcagca gaggtaatgt ctcctatggt agaccattca tacaattctg ttatagattt
     6361 aggtggagat aatgtaggtg ctagggttat aggaagattt agtcatttgt taaaagaagg
     6421 cgattatgat atgctttttg taataaatgc aaatagggag aaaactcaaa cttctgaaga
     6481 agttatccaa tatataaaag aaatagaaaa atcttctaaa ttaaaggtaa caggtctaat
     6541 taataatact catcttatta ggtttactac tattgatgat gttttaagag gtcagaaagt
     6601 tgcaaaagaa gtttctgaaa aatgtaatat accgataaga tatgttgcat gtttagaaaa
     6661 tcttgttgaa cagttaccaa aagatttaga aggagaaatt tttcctataa aattatatat
     6721 gagagaagac tggatgtagt ttgagctcaa atatatagaa agtttaagga ggcttaggta
     6781 aatggctaaa ggaaaagtat cttttaatca agaacgttgt aaaggttgtg gattgtgtgt
     6841 tgaagcatgt ccagtaaaaa tcatacaatt agactcaaat gtaataaaca aaaaagggta
     6901 taacccagca actgtttttg aaatggaaaa atgcatagga tgtgcaagtt gtgcaactat
     6961 gtgtccagat gtagttataa cagtagaaag agattaattt aattaatatt taagggggaa
     7021 ttttaatggc taagatactt atgaaaggta acgaagcatt tggaaaagca gctatagaag
     7081 ctggttgcaa atatttcttc ggttacccaa taactccaca aagtgaatta ccagaatatt
     7141 tatcaagaga gttacctaaa ataggtggag cgttcgttca agctgaatca gaagtttctg
     7201 cgataaacat ggtttatggt ggagcaggtg caggagcaag agttatgact tcttcatctt
     7261 caccaggagt tgctttaaag caagaaggta taacatatgc agtaggagca gaagttcctt
     7321 gtgttgtact taatgtaatg agaggtggtc caggacttgg aagtatacaa ccttcacaag
     7381 ctgactactt tatgtctaca agaggtggag gaaatggaga ctacagaact ccagtattcg
     7441 caccagctac agttcaagaa gctgttgata tgataatgga agcttttgac gttgcagatt
     7501 attacagatc accagttatg gtagttgcag atggtatgat aggtcaaatg atggagccag
     7561 ttgaatttag agctcctgag aagaaaagag aattgcctcc taaagattgg gcaactgttg
     7621 gaactaaagg aaaaagaaaa cctaatgtaa taaactctct atacttagag ccagaagtac
     7681 tagaagacca ttgttggcat ttacaagaaa aatttgatgc aatggaaaag aatgaagtac
     7741 aatacgaaat gtacaaaaca gaagatgctg aatttgtatt tgcagcttat ggaactactt
     7801 caagagtagt aaaaagcgca attgatatat taagagaaga aggaataaaa gcaggtctta
     7861 taagacctaa agtattatgg ccattcccat tcgaagcatt caaccaaatt cctaatgcta
     7921 gaaacatatt gactgttgaa atgagtatgg gacaaatggt ggaagatgtt aaaatggcag
     7981 tagaaggtaa attaccagtt tacttccatg gaagaccagg aggaatgact ccaacaccag
     8041 cagaaatagt tgaaaaagct aaaaaaatta ttgccggaga attggttgca ggaggtgcta
     8101 gataatggct gttgtattta agaaaactga aggattacaa gatactcaaa ctcactactg
     8161 tccaggatgt acacatggta taatccatag attagttggt gaagtattag aagaactagg
     8221 agtattaggt gatgctgtag gtgttgttcc agttggatgt tcagttttag gatataaata
     8281 ttttaactgt gacactcaag aagctgctca tggtagagcg ccagctgctg ctacaggaat
     8341 aaaaagagtt catcctgaaa atacagtatt cacttatcaa ggtgatggag acttagcttc
     8401 aattggtact gctgaaatag ttcatgcagc tgctagagga gaaaaaataa ctactatatt
     8461 tgtaaacaat acaacttatg gtatgactgg tggacaaatg gctccaacta cattagttgg
     8521 acaaagagct actactgctc aatcaggaag aaatgctgaa actcaaggtt atccaataag
     8581 agtaagtgaa atgttagcta cattaacagg agctgtattt gtagaaagag tagcagttga
     8641 tactccagct catgttagac aagctaaaaa agctataaag aaagcattcc aagttcaaca
     8701 agcaggatta ggatttggaa tagtagaagt attatctact tgtcctacaa actggggact
     8761 agctccaaac gatgctttac aatggttaag agacaacatg ataccatact atccattagg
     8821 aaatttcaaa aatgttgaag ttgaggaggt gaagtaaatg tctacagcta gagtaatatg
     8881 tgcaggcttc ggaggccaag gtgttatgtc tatgggacaa ttacttactt atgcaggaat
     8941 gctagaagga aaagaagttt cttggttacc atcttatgga ccagaaatgc gtggaggtac
     9001 agcaaactgt gcagtaactg tatctaatga gccagttggt tctccactta taacagatga
     9061 tgcaactgca gctatattat taaatatacc agcatttgaa aaattcaaag atgatgttat
     9121 tcctggtgga aaaataatag taaatagttc tttaataaaa gaaaaagttg atagaacaga
     9181 tgtagatgtt tattatatac cagcaaatga attagcagct gaattaggaa atgataaagt
     9241 tgctaacatg ataatgttag gcgcttactt aaaagtatct gatactgtag atatagaatc
     9301 tgtactagaa gcattcaaaa aagttttcgg acctagaaaa gaaaaattcg ttccattaaa
     9361 cagagaagct ttacaaaaag gtatggatgc tacatgttct aaagcaacta actaataaag
     9421 aaaaagatgt caaaaaaaag acaatagaat tatttctatt gtcttttttg ctgataagta
     9481 ttaaaatatt tttatgactg aaattaagcc tataatgata ttttatgaac tataaaaata
     9541 taatacaaat tacaagcttt tcaattatga aaggagaatc tttagtgaga aagtattttg
     9601 gaacagatgg agttagagga gtagcaaata cagaactaac atgtgattta gcatataaac
     9661 ttggtagagc tggtggattt gtcttagctc aaggagacca cagagtaaaa gtagtagtag
     9721 gtaaggatac tagaatatca ggtgatatgt tggaagcatc tttaatagca ggacttatgt
     9781 ctgttggatg tgatgttatt actgttggaa taataccaac acctgcagtg gcatatttaa
     9841 ctagaaaata tggtgctgat tgtggtgtgg ttatatctgc atctcacaat cctgttgaat
     9901 ataatggaat caaattcttt aataagaatg gatataaatt agatgatgaa attgaattga
     9961 aaatagaaga atatatagac gatatagaca aaattgattg tcttccaatt ggtgaaaatg
    10021 taggaagaaa attacatgaa cattgtgcac aaagagacta tgtagactac ctaaaatcaa
    10081 taataagcac tgactttaag ggattaaaag tagttttgga ctgtgctaat ggtgcgtcat
    10141 ataaagtggc accaatagtg tttgatgaac taggagcaag tgtaatttca ataaatagtt
    10201 caccagatgg aaataatata aactataaat gtggctctac acatccagaa caacttcaaa
    10261 gggctgtttt ggaacataat gctgacttag gtcttgcata tgatggagat gctgatagat
    10321 taattgcagt aaatgaaaag ggtcaaattg ttgatggaga ccatataatg atattgagtg
    10381 ccttaaattt aaagaaaaat aataaattgg cacaagatac tttggtagtt acagttatga
    10441 gtaatatagg gttgacaata gctgctaagg aaaatggaat aaatttatct actacagcag
    10501 ttggagacag atatgtttta gaagatatgg ttaaaaatgg atataatctt ggtggagagc
    10561 aatctggaca tatgatattt ttagattaca acactactgg agatggagtg cttagttctc
    10621 taatacttgc aaatataatc ttgcaagaaa agaaaccttt atcagaaatt gcatcaataa
    10681 tgagccaata cccacaagta ttagtaaatg caacaataaa aaatgaaaat aagaacaaat
    10741 atatggaata tccagaaata aaaactgaga tagaaagaat agaatctatt ttagacggaa
    10801 atggtagagt tttgataaga ccatcaggaa ctgagccatt agttagagtt atgttagagg
    10861 gtaaagagga aggtcaaata aaagaattag caacaaactt agctaatctt atacaagaaa
    10921 aattatcgta atttaaaggg tattctcaga tataaatact gaggacacct ttctaagcgc
    10981 cagaacttag ttatagacat aattattgag taatatagga actaagttga cgaggtcaga
    11041 gttaatcgaa ttgtcggcgg atgctctgcg gtgtgtttac catcgaaaga cttctttaaa
    11101 taataggagc aatcctatgg acaaagggaa gccaaagtaa acttatatga cctaactctt
    11161 aaaatatata aataatattt ttaggaggac acaaataatg tgtggaatag ttggatattt
    11221 aggaagtaga aaggcagcag aagttatagt agaagggctt tctaagctag agtacagagg
    11281 ttatgactca gcaggagtag cagttaatag ttctaatgaa aaagaactta atattagaaa
    11341 gtttaaaggt agactttctg tacttgcaga agacttagaa aagaatccta ttgatggaaa
    11401 tttaggtata gggcatacga gatgggcaac tcatggagaa ccatctgatg taaactcaca
    11461 tcctcatttt aatcaagcca aaacaatagc agttgttcat aatgggataa tagaaaacta
    11521 tatggaaata aaagaagaat taatttctga aggtgttaag tttgaatctc aaacagatac
    11581 agaggtaata gcacatttag tagataaata ctatgagggt aatttattag atgctgtata
    11641 caaaactata tcaaaattaa gaggagctta tgcattaggt gttatatgta aagaacatgg
    11701 aaatgaatta gttgcagtaa gaaaagatag tcctcttgta gttggtgttg gtgaaggtga
    11761 aaactttata gcatcagata tacctgcatt attaaaatat actagagatg tttatttctt
    11821 agaaaatgga gaagtagttc atctaaaaga cgaaaatgta acagtttatg actctaatag
    11881 aaatcttgtt gaaaaagaag tatttcatgt aacttgggat gtagaagctg cgtctaaagg
    11941 tggatatgac tactttatgt caaaagaaat tcatgagcaa ccaacaggag taagagaaac
    12001 tttagaaaga agacttgatg ataatggaaa tataattcta gacagtataa atatttctaa
    12061 agaagactta gaaaaaataa ataaagttta tatagtggct tgtggaacag catataatgc
    12121 tggattactt ggtaagtatg ccatagaaaa atttgtaaat ataccagtaa taactgatat
    12181 agcatcagaa tttagatata gcgacccatt tgtagatgaa aattcattag taatattagt
    12241 tagtcaatct ggtgagacag cagatactct agcagtatta agagattcaa aggctaaagg
    12301 agcaagaata ctttcaataa ctaatgttgt aggttcatct atagcaagag aatcagatga
    12361 tgtattctat acttgggcag gacctgaagt agcagttgca tcaactaagg catatactac
    12421 tcaaataact tctttataca tgatagcttt agactttgct atcaaaaaag gaacaataac
    12481 tagagaattt tatgatagta tgatttctaa gatgaaagaa ataccatcaa aaatacaaga
    12541 aatactagac aatgaagaat atataaaaga agttgctaaa acagttgtta gttctgaaca
    12601 tgcattctat ttaggaagag gtatagacta tagcttagca atggaaggtt cattgaaatt
    12661 aaaagagatt tcatatatac atgcagaagc ttttgcagca ggagagctaa agcatggaac
    12721 aatagcttta atagaaaaag gaactcctgt aattgccata gctactcaag aaaagttatt
    12781 tgaaaaaatg gtttctaata tggaagaagt aagagcaaga ggagcttatg tagtagcaat
    12841 agctcaaagc cataataagg atgttgagaa agctgctgat aaaataatct atataccaaa
    12901 ttcagatgat attttatcac caatattagc agttgtacca atgcaattac ttgcttatca
    12961 tgtctctgta ctaagaggat gcgatgtaga taagccaaga aatctagcta aatcagtaac
    13021 agttgaataa aaacagatta aaaattaaat agataatatt tattgaaaag aactcatcct
    13081 gttatttgta ttttatagga tgagtttttt agataaatat taaatttgtt ttgtttttgc
    13141 tataataaat tttataaaaa gattgatatt aaatcatctg taaaatgata aatatataga
    13201 ccataccagg tatagggaac gataaatata gttcatataa aatatagtga gtacttaata
    13261 aacatatagt caatatcaga taaaaaaata atagcttatg agaaaaatta aaatacaagg
    13321 tattagtaac cttgtgataa gataaagtga gtagtttaaa ataagaatat aaggaactat
    13381 ttataaaaga atataatgaa gctaatagag tataagaaaa ttactttatt ttagtaaaat
    13441 aatttcaaat aaaaattttt ttaaattggg agtataaatt atgacaaatg attttaaaaa
    13501 agaaaagaaa gaagattatt ttgttaatct taaggcaata tctactgaag ttttacaaga
    13561 aaaagtgcaa gataacgctt gggttatagt tgatactcgc ttaaatgatg cttataatgg
    13621 ctggaagtta gatggagtaa aaagaggtgg acatataaaa ggagcagtag atttttctgc
    13681 taattggtta agtgtatact cagatagaaa agatgaagtg ttagaacaag cattgaaaac
    13741 taaaagaatt gatttagata aaaatatagt attgtatgat gcaaatggaa aagatgcttt
    13801 ggttgtagca gattatttat ctaaaaaagg atataaatat ctatataaat atgatattaa
    13861 acaatgggca gacgacgaaa atcttccaat ggaaagatat aaaaattatc aaatgatagt
    13921 tcctgctttt ataataaaag atatattaga tggaaaaatt cctgagacat ttgaagattc
    13981 aaagaatata aaaatgattg aagcgagttg gagagaagaa tcgtatacaa aagggcatat
    14041 acctacaagt gttcatgtaa atactgatat tatagaacct cctccaacat ggatgcttga
    14101 taatgatgat aacttaacta agtttgcttt agactatgga ttaacaaagg atgatactgt
    14161 aatagtttca agtagtacac ctatggcatc ttatagacta gctgttatat tgagatatat
    14221 aggagtaaaa gatgtaagag ttttgaatgg tggaacaaac tcatggttat cagcaggata
    14281 tgaattagaa tttattagta atccaaaaca ttcatgtact aattttggag cagatatacc
    14341 agttaactca caattaattg ttactacatc tgaattaaga caaaaactaa aagaaaaaaa
    14401 taagtttata ctagtagata accgtacttg ggatgaacat ataggaaaag tatctggata
    14461 tacatattat gataaaaaag ggcgtatacc aggagcttta tatggacatt caggtagtga
    14521 tagtgtttca ctagaagaat atcgtaatat tgataataca atgagaaata aatatgagat
    14581 tttagaaatg tgggacaagg aaaatataga tgtgaataaa cagcttatat ttatgtgtgg
    14641 aagtggatgg cgtgctgctg aagtattaac atatgcaaat gtcataggag tggaaaatac
    14701 ctccctttat agtgatggat ggatgggctg gagtcttgat aattcaaatt taattgaagt
    14761 gggagaacac aaataaagtt tttgaaagtg caatagagtt gtttaaaaca aaggtgtttt
    14821 tgatttatta ggaagagtgt gtaaattgtg tattgggaag tgtacttagg tatgttcatt
    14881 tggagctatg gaaattgata aaaaagtatt atctgtttat tttgttggta aaatatgaat
    14941 agattataga ttatagatta tagattatag attatagatt taagtataga ctaagtaatt
    15001 tatattacag aagagaatat ataagaatta atgcaagatg tatttgatac atataagaaa
    15061 ttggcaagtc caggagaagg atagagaaaa ttgagattga agagtttgaa agagattatt
    15121 agatgaaaaa tacaaaaatg tataaaaata catattgaga aagtatatct ctcttaaatt
    15181 tgttaataat tctattatac agaaaaacat atttaagggg gattttttac tatgaaaaaa
    15241 gtaaaattag ctataagctt ttttatatta atgattatag gaatgacaat atcttatgca
    15301 gatacaagta taaatactag atacaataga ttaagtaaag ctcttgaaat atcagaagca
    15361 aattttaaat tttataatgt taaaattaat tctgttataa atcataattt atcaaataaa
    15421 gaatttcata atatttttat agaaataatt aacaatctaa agttggataa aaaaggaata
    15481 aattggaatc aaaaagggtc tggcgataaa atacaattat gtgctactat aaaaggtagc
    15541 gataaagaaa tttctataaa aggaataaat aaaaataaca aagaaagtta catagtagtt
    15601 gacatattgg ataacaaagt atataaacat aaagaggtta tttatagtaa cgtagaaaat
    15661 gtgttaaata aatattcatc tcaagtagat gtgtctgctt gtattgttgg agaatacaca
    15721 aaaaggttac aaaacaacaa atatgatgat attttacaaa aaatattata taatatgagt
    15781 gctaagcaaa tagatgaagt taaagacgta aactttttat ctataacagc atatagtaaa
    15841 gtattaaatg attatgaatt agaatattta ggggaaaaaa ttaatttaaa tataggaatt
    15901 aaatataatg aggatgacgg aaaaacactc atatatattg ccacaccaat tataaaatta
    15961 gattattaat tatgaggaga tcaaaaaata tggctaagat aatagtaaaa aagagtaatc
    16021 cacttaaagg tagtgttaaa atagatggtg caaaaaatgc agttttacca ataatagcag
    16081 cgactttatt agcaaacgga aaatcaacat taaatggagt accaaattta agagatgttc
    16141 atgttatatc agatttatta agacatgtag gagcagaagt tgaatataaa gaaaatacac
    16201 ttactgttga tgcaagcaat ataaaaactt gtgaagctcc atatgagctt gtaagaaaga
    16261 tgagagcttc ttttttggtt atgggaccat tgcttgctag atttaatagt acgaaaattt
    16321 ctatgccagg aggatgtgct ataggaacaa gacctataga tttacattta aaaggattta
    16381 aagctttagg tgcaaagata gaaatggacc atggttttgt tgaagcagca acagaaaagt
    16441 tagtaggaaa taaattatat ttagatttcc catctgttgg agcaactgaa aacataatga
    16501 tggcagcttc tttagcagaa gggactacaa taatagagaa tgcagcagaa gagccagaaa
    16561 tagtagattt agcaaacttc ttgaacgaaa tgggagcaga tgttaaaggt gcaggtacta
    16621 atactataaa aataaaaggt gttaaagagt taaaaggagc agaacataat gtaattccag
    16681 acagaataga agcagcaact tatatggttg cagcagctat gacaaaaggt gatataactg
    16741 ttgaaaatgt tttaatggag catttaaaac cagttgtagc taaattaaga gaagctggtt
    16801 gtgaaataac agaaatggac aattctgtaa gagttgtagg tccaaaagta ttaaaaccaa
    16861 tagatataaa aactttacca catccaggat ttcctacaga tgttcaagca caatttatgg
    16921 ctatgcttac tgtagccaat ggaactggtg tagttataga aactgttttt gaaaacagat
    16981 ttatgcatgt tgctgaattt aatagaatgg gtgctaatat aaaaatagat ggaagaagtg
    17041 cggttgttaa tggtgtagat gagctacacg gtgcagcagt aaacgctact gatttaagag
    17101 caggggcagc ccttatttta tgcggtctta tagcagaagg agaaactcaa ataggagaaa
    17161 tataccatat ccaaagaggt tatgttgata tagataagaa gataacagca ctaggtggtc
    17221 aaatcgaaat agtagaagat taataattgg tttatagaag aaagttataa attaataggg
    17281 ttgttcatat acttaaatac aaggttttaa aagctaagga ggagagtata tgaagaaccc
    17341 attggttgtt ttgttaggat ttgtgacttg ctctgtgtta gttccatcac ttataacttt
    17401 agtttcttat aaaaatgtag aattaactga aaaaccagag agcccagtat ctataaacaa
    17461 aactattaaa aaaagtgata ttgaagacaa aggtaataaa gaagaaaaaa atacagtgaa
    17521 ctatgaaact gtaaataaaa aagcaccaat tatcaatgta tataatcata taacagggaa
    17581 aaccgaaaag atggacatgg agaactattt atgtggagta ctagctggtg agatgtcttc
    17641 agagtttgat atagaagctt taaaagctca atctgtagca gctagaacat atgtagtata
    17701 taaacaggaa catggtaaat ctagtaaaca taaaaatgca gtagtatgta ctgattataa
    17761 acattgtcaa gaatataaga gttatgatac tcttaaaaaa ttaaatggtg aagaatggat
    17821 taagaataaa tattccaaaa tccaagaagc agttagagga actaaaggtc aaataataac
    17881 ttataatgat aaggcgatac ttcctcttta tttttctaca tcttcaggaa aaacagaaaa
    17941 tagtgaagaa gtattttctg caaaatatcc ttatttaaag tctgtggaaa gtccttatga
    18001 taagtattcc cctaaatttg cgtcaacact taaaataagt aacacagatt ttgttaaaag
    18061 tcttagaagg gcatatagca ctattgtgat agatgtgaat aatttaagta aacaagtttc
    18121 aataactaaa agaagtgatg ccggaactgt agaaaaaata aaattaggaa ataaagaatt
    18181 gactggaaaa gacattagaa ctgtttttaa gttgaactct gcaaactttg acataaaatt
    18241 tggagaagga tacatagatt tcgtggtaaa aggatatggt catggtgttg gtatgagcca
    18301 gtggggagca gaaggaatgg cagaagaggg ttataaatat tatgatatat tatcacatta
    18361 ctacacagat acaaaaataa aagatatata ctaataattg tataaaatgc caaaaacacg
    18421 tcaataatcc aaatgtagaa atatatttgg aggtgcaaaa tgaagaaaaa gctgttagaa
    18481 aaagatggtt tttatttatc tttgtttgta tgtgtttgtt tattagcagt aggtggagtt
    18541 tggtttacaa ataataatgt agataaattg gcttctaata aaggaattat ggaaaatgct
    18601 aataaagata gtgaagagga aatacattta attgaaaaag acaagaaaga tgctatccct
    18661 actgcaacgg attcaaaaca aaacttagaa aaagctaagt caaaagagga aaataaatcg
    18721 agtacaacaa aattaaatta cattggagat aaagtaataa gaggatattc agaaaaagag
    18781 cctagttatt ctaaaacact tgatgtttgg gaaactcata aaggtgtaga tattagttgt
    18841 actaaaggca aagaagtaaa atctctatta aacggaatag tagtagatgt atttgatgat
    18901 gaagaatatg gacaatcagt caaaataaaa agtgacaata acattgtagt agtatattca
    18961 aatctagata aaaatgttag tgttaagaaa gaacagaagg taacagaagg acaatctcta
    19021 ggtactgtag gtagtacttc tcaaatagaa agtgaagaag gtatacatgt gcatttagag
    19081 gcgtatagtg gagaaaaatc tatagaccca atgagtctaa ttaagtaata gtataacaag
    19141 tttaatcata tttggttaag cttgttatat tttttggtgt ttttacatat atatcattaa
    19201 agctattatg tgaggggggt aatctttgag atctcatata gaggaaaggg ccatagtggt
    19261 agcaaaatat atacttgaaa aaaacactac agtaagacaa accgccaaga catttggagt
    19321 aagtaaaagt acaattcata aggatgttac agaaagattg aaggaaataa atccatccct
    19381 tgccaaagaa gttaaaaatg tgctggacaa aaataaatca gagagacata ttagaggggg
    19441 attagcaacg aaattaaaat atgagaagga acataaaaaa atgtaggtta aaaacctact
    19501 ttttttattg aattaaatta taaaaagaaa tatattaagt aattataaat agcaaaataa
    19561 taatactctc aaaagattta ttttctcttg attaaagcga gatttttgta tatgctttat
    19621 tataggagta ttaaaatagg cttataatat aatctaatat tataaataaa ggagttgaaa
    19681 aaatggctgg agctgatata ggcatagatt taggtactgc aaatgtgctt gtttatgtta
    19741 gtggtaaggg aatcgtttta gaagaaccat ctgttgtggc gatagacaaa agaacagatt
    19801 ctgttttagc tgtgggagaa gaagctaaaa agatgatagg gagaacacca ggtaatatag
    19861 tcgctataag acctttaaga gatggtgtaa tttcagatta tgatgtaact gagaagatgt
    19921 taaaatcttt tatagataag atagttgata aaaagggatt tggaagattt tttatgccaa
    19981 gaattatggt atgcgtacca acaggtgtca cagaagtgga aaaaagagca gtagaagatg
    20041 ctacgagaca agctggagct agagaagtat atatcataga agaaccaata gcagctgcca
    20101 taggagctgg tgtagacata tcacagccta atggtaatat gattatagat attggtggag
    20161 gtactgtaga tatagctgta atatctctag gaggagctgt tgtaagtgaa tctataaagg
    20221 ttggtggaga tagatttgat gatgctatag ttaaatatat gaaaaaacaa cacaatcttc
    20281 ttataggaga aagaacagca gagaaaatca agtttgaaat aggttctgca tttaagagag
    20341 aagaagaaaa atatatgaag ataacaggaa gaaatttaat tacaggactt cctagttcta
    20401 taactataaa ttcaacagaa atgttggaag cacttagaga atgtgttgag caaatagtag
    20461 ttgctactca tgctgttttg gaaaaaacgc ctcctgagtt agcagcagat ataggagatg
    20521 tcggtataat aatgactgga ggaggttcat tattatatgg attagataaa ataatagaac
    20581 agaggacagg aataactgtt acaatagcag atgaaccatt atcttgtgtt gcaaaaggta
    20641 caggaatagc tttaggttca atagacttac ttgaaactgg tgggtctttc aaaagaaaat
    20701 aattaggaga gtttagaatg ttaaatatag atgaaataaa aaaattaata ccacatagat
    20761 atccattttt attagtagac aagattacag agctagaagt tgggaaaaga gctgttggta
    20821 taaagaatat aacagttaat gaaccatttt tccaaggtca ctttccagaa tatcctttga
    20881 tgccaggagt actaatagta gaagctctag cacaagtatg tggagtggca atgatgtctg
    20941 tagaggaaaa caaaggaaaa ctaggtgtat ttgcaggtat agataaagtt agaatcaaga
    21001 gagaagtaag accaggagat acattaacta tggaagttga aatgacaact cttagaaaaa
    21061 atatagctaa ggcagatgct aaggcatatg taggtgaaga acttgtctgt aaaggtgaat
    21121 taatgtttgc tttagtagaa aaataaaaac ttgaaatata aaagtaaaaa ggtagataat
    21181 ttggagtatt taatcagata ctctaaaata tctacctttt tatataaaga attttatcat
    21241 ttagtttata tttagattag gtggatacta ataaagccaa ggctattgtt tcagctaaat
    21301 ctaatataaa gctaaggcgt atattattaa atgaaagttt attattttca tcaattttat
    21361 ctacaattcc tactatagaa taatttccta tttgaggtag ttttttacca acaccttttc
    21421 cgggcaggat aggtccttct ctaatctgta tattacctat gttactctgc gaccccaagc
    21481 aagcatctat agccaagaag ttaccttgta tatgtgtatt ttttatagta tctaaagact
    21541 catatatatt taaggcgtga atagggttat ctagtgtacc gtaaacgggg tatttaaagt
    21601 tactgttctt aagtattgtt ccaactaaag ggcctaaagt atcacctata gccctgtctg
    21661 ttcctatgca aacaacaatc gtattttcat taattatagt ttttagaact gaacttagca
    21721 tctgtataac atcatcatcg ctgtagttta ctttagctaa atacaatagg aacaccccct
    21781 tcaaaaaaat atatgaataa aaagcattaa aaagacctaa ctttaaattt cagatagata
    21841 gaaaatttta tttgtcttac aaaaaaagga atagaaaaaa tatgactaaa aatattgaca
    21901 aaattttaaa aaaatagtaa gctagtaaag taaaacaaaa cttaaatctt gattacctta
    21961 tcaagagagg ctgagggata ggccctgtga agcccagcaa ccacccaaat gggaaaaggt
    22021 gctaattcct acaagacaaa agtcttgaaa gatgaggtca gttaattagt atgtaattac
    22081 gattattaac ctcttcttat aaaggagagg tttttttatt ttgagtaaat ttctaaaatt
    22141 aaaaacaagg taaaacctat aataaaaatt aagaataaat aagggggaat aaaaatggca
    22201 agacatttat ttacgtcaga atcagttaca gaaggacatc ctgataagat atgtgaccaa
    22261 atatcagatt caatattaga tgcattatta gaaaaagacc cacaatctag agtagcttgt
    22321 gaaactacag ttactactgg tttagtttta gtagcaggag aaataagtac atctgcttat
    22381 gtagacattc ctaaattagt aagagaaaca gtaagagaaa ttggatatac aagagcaaaa
    22441 tatggatttg attgtgatac ttgcgcagtt ataacttcta ttgatgaaca atctggagat
    22501 atagctatgg gagttgatga gggtctagaa agtaagactg gagaagagat agaagaagaa
    22561 atagaaaaag ttggagctgg agaccaaggt ataatgtttg gatttgcatg taatgagact
    22621 ccagaattaa tgccacttcc gatatctttg gcacataaat tatcaagaag acttacagaa
    22681 gttagaaaaa caggtttagt tgattattta agaccagatg gaaaaactca agttactgtt
    22741 gaatatgaag gaagtaaagc tgtaagagta catacagttc ttatatcagc tcaacattgt
    22801 gaaacagtat caaatgataa aataagagaa gatttaatta atcatgttat taaggaagtt
    22861 ataccagcag aattacttga tgaagagact aaaatttata taaatccaac aggaagattc
    22921 gttataggag gaccacaagg agatacaggt cttacaggaa gaaaaataat aatagatact
    22981 tatggtggat attctagaca tggtggagga gctttctcag gaaaagaccc tacaaaagtt
    23041 gatagatctg ctgcttacgc agctagatat gttgcaaaaa atattgttgc ggctggtctt
    23101 gcagacaaat gtgagataga acttgcatat gctataggta tagctagacc attatctata
    23161 tttatagata cttttgggac aggcaaggtt tcagaagaaa aacttgttga attagtaaat
    23221 aaacactttg atttaagacc gggagcgata ataagagact tagatttaag aaagcctctt
    23281 tataaaaagg ttgctgctta tggtcacttt ggtagaactg atatagattt accttgggaa
    23341 agaactgaca aggttgagca gttaagaaaa gatgctttag gtgaataatt tttaactaaa
    23401 aagctgttag aatgtaattt tacgattact ttctaacagc ttttttatta tgagttaaat
    23461 atttttaata aatgttaatt tatcatcata taatgaataa acagtattta atctggtcaa
    23521 tacattttat ataatactct actaaattta tacatattat gggatatatg ttaaaggatg
    23581 gttaagaatg tgttaactaa tgttaaataa ttaaaaatat attgaaatat cacaataatg
    23641 agtctaatat aatcttaata aacttacact ttaaaaataa ttttaattgg ggggagtagt
    23701 gtatgtcagg caaagtaaat attaaaacgg ttataagttt tgctggagct tatgttgcta
    23761 ctgtaatagg ttctggattt gcaactggtc aagaaatact gcaatttttt acgttttatg
    23821 gatatgcggg tattataggt ggaataatct ctatggtact attttcatgg tttggagcag
    23881 aagttataga taagggaaga gaattgaaac ttaaggagcc aataaaaatt tatcaagtat
    23941 attgtggaaa atatcttgga acattttttg aatggtttgg tccattgttt ttatttggaa
    24001 cattagtaat aatgattgca ggagcaggag ctacattatc agagtactat ggattaaatc
    24061 catatgtagg taggattggt atggcaatcg tttccctaat aactgtttca ttaggtttga
    24121 caaggctttc taaaatacta ggtaatatag gacctataat aataatattt acacttttag
    24181 taggagcaat tagtttattt agcaatattg atgctttatc aaatgctgga aatatggttg
    24241 atagtttaaa tattaaaact gctacatcaa atggatattt ctcgggagta ctttatactt
    24301 gttacaatgt aataatagta ataacattct tgacaggaat gggagcttct gcagtaaata
    24361 agaaagatgc tgtatggggt ggtatagtag gtggagtagc acttatggca gcagctataa
    24421 tgatgaattt ggcattactt tcagacatag gaaatatata cacaaaagag ataccagcac
    24481 tttacttagc tgataagata tctccaataa taggtatttt attctcagta gtacttttac
    24541 taggaatata cactacagct gtaccattgt tatggtcagt aacaaaccga tttgtagaag
    24601 atgaccaccc taagtttaaa attataacaa tagtggtaag tatattagct tgtataggag
    24661 gtttactgcc atttgataag ttagtaggta cactatatcc atatacaggt tacatgggaa
    24721 ttttaatttt actatgtatg ctttatagaa gaattactaa aactgagggg tacaaggaga
    24781 ataaatctga aataagttag tactgtaata tacttatgaa atttaattta tactctaaat
    24841 atagagtaat taaaataaaa tagctattta aatatggtac ctgtatttca tggtaccata
    24901 ttttatggat gcagattttt tttgtttctg ataaaatgta taatagttgg aaattacgtt
    24961 aagtataaaa atataaaact tttctacgat tatcatagtt ttaatatata aaattatact
    25021 aacatttaaa tggagcgatt attagtattt ttaggagata aaaatggaaa aattagaagg
    25081 aatgataagt gaaatagtct ttaaaaacga agataatggt tatacaatag ctcacctggt
    25141 aaatgaaaat gatgaaattg tggttgtagg atgtatgcct actttagcaa ttggagaaag
    25201 catagaagtt gaaggaaagt gggtaaatca taagatttat gggactcaat ttgaagtgaa
    25261 tagttttatg ccagtaacac catcatcctt agaaggaatt tatgtttact tatcttctgg
    25321 aatgatacat ggtattggtg aaaaaatggc aaaaagaata attgataagt ttggtgtaga
    25381 tacgttagag gtgatacaaa attcaccaga aaaactacaa gaagtagaag gtatagggag
    25441 taaaaaagta aaacaaatag taaaaagtta tgaagaagac agagaattga gaaatataat
    25501 aatacagcta tctccttttg gaataacacc aaattactgc ctaaaaatat ataaaaaata
    25561 taaaagcagt gcaatagaag ttataaataa aaacccatac caacttgctg aagatataag
    25621 aggaattgga tttaaagtag ctgatagtat agcaagtaaa attggtatag ataaaaactc
    25681 taaagataga atttgtcagg gaatcttata cactttaaat aaatctttaa gtaatgggca
    25741 tacatattta ccagagcatg ttctaataca agattcggaa aaattattgg agctaaatgg
    25801 agaaataata aaagagtgtg taatgatgct agtttataat caaaaaatac atatagagaa
    25861 agtaaacaat gaaaatctta tataccttat gccatactat ttagctgaaa atggagtatg
    25921 tagtcaaata gtaaaattat cacagtatga atttgaagac ttaaaaatag atatagataa
    25981 cgagattaat gttttagaag aagataagaa aattaagtta gcagaaaaac aaattttagc
    26041 agtcaaagaa tctgttaata gtggagtatt aattataact ggtggaccag gaactggtaa
    26101 aacaactacc attaatgcta tcatagatat atttgaaaat aatggtaaaa gtgtgactct
    26161 ggcagctcca acgggaagag cagcaaagag aatgagtgaa acttctaata aagaagcaaa
    26221 aactatacat aggttgctag aaatgggatt ttcaacagat gatgatttaa ctttttttaa
    26281 ggatgaagaa gacccaataa attcagatgt aataattgtt gatgaggtat caatggtaga
    26341 cattatctta atgtacaatt tacttagagc tataaagtta ggaacaagag taattttagt
    26401 tggagatagt gaccaattac cttctgtagg tgctggaaat gtacttaagg atatgattaa
    26461 ttctaatatc ataaatgtag ttaaattaaa tgaaatattt agacaagcgc aagaaagtat
    26521 gataattgta aatgcccata agataaacaa tggagaacca ctttatctaa atacaaaagg
    26581 taaagatttc ttttttataa gaaaatcaac taatgaagaa attttaaatg aaataatagg
    26641 attggttaat gaaagattac ctaaatttta taaagttgat aaattaaaag atatacaagt
    26701 tttatcttca atgagaaaag gtgaattggg agttactaat ctaaatatag agttacagaa
    26761 gtatcttaac aaaaaagaaa aatttaaagt tgaagaaaat ttttctaaaa gattatttag
    26821 agttggcgat aaggtaatgc aagtaaaaaa taattataca aaaaaatggg aaacagaaga
    26881 ccaaaaagaa agtggagaag gtatctataa tggagatata ggatatgttt atcacataga
    26941 taaagataag aaaactattt atatattatt tgaccagact aaaattgtat catacctata
    27001 tgatgaatta gatgaaattg atcatagttt ttgtacaaca atacataaaa gccagggaag
    27061 tgaatttcca gttgtagtat tgccaatagc gtgggctcct cctatgttac ttagccgtaa
    27121 tttattatat acagcagtaa caagagcaaa aaaattagtt gtattagttg gagatgtgaa
    27181 atatcttgaa tatatgataa aaaataatag agttaatcaa aggtattcaa atttaggata
    27241 taagctaaat aaatttaaac aagaagggtt gttgatagaa taaatatgaa aatatgcaat
    27301 gataatttta ttaagaattt taaaaatatg attaataaat gtttggattt tatatatcct
    27361 gagaatataa gttgcataat ttgtgataaa tccataaaga aaacaaatac ttattcaata
    27421 tgtaaaagtt gttttaaaga aatgaatttt atacaagatg gttgcatgaa gtgtggaaaa
    27481 ccaataatac gtcattcaat tgaaaaagaa tttattgaag agtgtagtta ttgttttaat
    27541 aaagactttt attttgataa atcaatatct tgtattgaat acaatgatgt gacaaaaaag
    27601 atggtgttag ggctgaagta taaccaaaaa acctttatgg ctaagtatat agctcaaata
    27661 atgaaagaaa aattatattt agaaaacata aaatttgatt atatattatt tgttccactt
    27721 cataaaaaaa ggctaaataa aagaggtttt aatcaggcac aaaaaatagc atttaattta
    27781 agtaaaatag taaatattcc attattagat tgcataagta gaaaaaaata tacaagaatg
    27841 ctatataaat tgaataagaa agaacgaaaa gaagaattaa aaaatgtatt tgttgttaaa
    27901 gaaaatgtta aattaataaa taataaaaat atacttttaa tagatgatat atttactact
    27961 ggtctaacaa caaacgaaat ttctaaactt ttaaagttat caggtgctaa taaggttttt
    28021 gtattgactc tacttacaaa agcaaatgat aattatatta tggaataatc ttatatattg
    28081 acaattttag tttaaaatat taaaatgaat aagtgatatt taggtctgtg gttgaaagtc
    28141 caagccagtc gcaggcaaag ggatccacgt aagttaacac taaaaattgg gttaatgatc
    28201 atggtgcggc ttagaagtaa gacctaccga taataaaatc gagagagagt agtagtgcgg
    28261 cgaacccaag cgaaccctcc agtaggcgag tgtggggtca aaaaccaggt cagctaaata
    28321 tcatattaaa agagagctag cataccttta tgtatagctc ttttttatat ataataaatt
    28381 taggttgagt cataaagagg tgatatgttt gaataaaaga caagaaaatt tattaacaac
    28441 attgctatta aaagaagagt ttataaatgt taataagatt gccaatgatt ttgagtgttc
    28501 agaaaggaca ataagaaatg attgcaaata tattgatgaa tggttatcta ctttttgtaa
    28561 tgcttatatc aaaagaaaac ctaatattgg gattaaattt agtggagatg gaacagacaa
    28621 aatactggta aatcagaagt tgcgaggtga agttaaaaga gtaaatagtg agcttcataa
    28681 gcaagttgag atactaaatt tactactttg taataataaa aaagttactt taaatgattt
    28741 atctaataag ttgtacataa ataaaaatat tgtcagagaa gaaataagta aattatcagt
    28801 agcattaaat aattataatt taaatatctc gactaaaaaa ggcagtggta tatatattga
    28861 aggggatgag aaagacataa gaataatgtt agtatcattc ttatttaaat atatagataa
    28921 atacagatta aacatagatg aaatagagta cttccatata gtagatataa taattgtaaa
    28981 aaacataatg cttactatag aagaggatat ggatataaga cttacagata tatcattcaa
    29041 gcaattgatg ctatttttgc ttataaatat aatacgaata aggctgggaa attctttaaa
    29101 agtatcaaat actaagaaag agcttaagct aaaaatgctt atattggata tagaagatga
    29161 attgaaatct aatttatcca tagtattaaa ttatgaagaa aaattgttta tagaatcttt
    29221 aatattgggt atgaacaaac aaataaatag taataatatt aagaaatctt tatatctaaa
    29281 tgaagaatta gcaagttata caaaaaaaat tataaattta gtatcagaag aatcaggaat
    29341 aaattttaat aatgatatat tactttatga acaattaata tgtcatttga atgttacaat
    29401 gcatcaaatg aaaagcaatg tttacttaga aaatccactt ttagataaca taaaaactaa
    29461 attttgtttt ttatttacag taatatcaag ttcaatagaa attgaattaa aaggaaaaca
    29521 aataacggaa gatgaaattg gttatcttac acttcacttt caaacctctc tagaaagaaa
    29581 atacagcaaa agggaatcaa ataaaaaggc aagtattgta tgtccattta gttttggagt
    29641 aagtatgcta ttaaaagtaa agatagaaaa gagatttgat aacataaaaa ttattgaaac
    29701 attaagagag gaagatttaa aaagagataa ctttaataag actatagatt ttataattgc
    29761 atttcaagag tatgaaaata tccaaaaacc aatttttata acaacaccat tatttactga
    29821 tgaggatgag aataaactta aagagttcac aaacaaaata aaagaaaaag acacttcata
    29881 taaacttatg aacagattaa tgatgtctga ttatttaata agagagttag aacatgatga
    29941 tatttatgaa gctataacat atttaactaa tttattaatt gaaaaacatt atgcagaaaa
    30001 agagtattta caatctatta taacaagaga aaagtcatat ccaaccaatg ttggaaaagg
    30061 aatattattc cctcatggag acatgaaata tataaaacaa tctgttttat gttttgcaag
    30121 gttaaaaaca ccaataaaaa ttagaaatgg aaaggatata aagattatac ttttgttagc
    30181 gtataaaaaa gatgatgata gaaaagtatt tagagaatta tttaaagaaa tatctaattt
    30241 aacagaggat gaaaatatgc tagatattct aagtaaatgt gacattgaaa aggtaaaaga
    30301 tattcttata taaaattcat agtgaactat ggataagcac aaaattttcc atgtgatgga
    30361 aaaagttgtg catgtttttt tgtttttata agatattata tgtttgtaag gtataaataa
    30421 tctaaaagta gagaagcaac ttaggaggaa aaaatggaag aaatagtttt aaagattata
    30481 attcatgctg gaaatgcaaa atctatgctt tatgaagcac ttgactatgc aaaggaaaac
    30541 gattttaaaa aagcagatga attaattgaa aatgcaaatg aagaaatttt aaaagcacat
    30601 aaagtacaaa ctgaacttat acaaaaggaa gctgggggag ataaatcaga tatatctatt
    30661 ttattgattc actctcaaga ccatttaatg acttgtatga gtgagagaaa ccttataaaa
    30721 gaaatgatta tgttaagaaa agaaattcaa aaattaaaat aaaaaaatta agggggattt
    30781 aaaaaatgaa aagaaaagta tatttatttt gtagctttgg tatgtcaaca agtttacttg
    30841 cagataaaat gcagaaggta gcagatgaac acaatttacc aatagaagta gaggcatttc
    30901 cgatagcaga aattgacaaa atagttgaag aaaaaaatcc agattgtata ttattaggac
    30961 cacaagttaa atacatgcta aaggagttaa aaccaaagtt tgaagctcaa ggaaaactta
    31021 tagatataat caatgaagtt gattatggaa ctatgaatgg ggaaaaagta ttaaaacttg
    31081 ctataaagct aataaaatct aaacaagcgt agaattatag taaaaaataa taggaaatga
    31141 gggtggatag atgaatagat tggaaagaat acttatgcca ttggctgaaa aaataggtaa
    31201 aaataaatta ttaattgcaa ttagagatgg ttttttagta tcatcaccat tattaataat
    31261 aggttcatta tttttattag tagcaaattt tcctataaaa ggatggaatg aatttgtagg
    31321 tcaaattttt ggtccagatt gggctgtaaa attgcaacaa ccaactgtag caacatttga
    31381 agtaatgact ctacttgcag ttttagggat tggatattct tatgcaaagc aaatagatgt
    31441 agacccaata gcatcagcag cagttgcaat agttgccttt tttataataa caccatttgt
    31501 aataccatat acaccagaag gaacagaatc agtttacatg gtaacaggta taccactagg
    31561 ttggatgggt tctaagggaa tgtttgtagg tatgataaca gcaataacct ctgtaaaatt
    31621 atttgctgca gttgtaaaaa aaggatggac attgaaaatg ccagatggag ttcctccaac
    31681 tgtatcaaaa tcatttgcag cattaatacc aagtgcaata gttatggtag ttttcttttt
    31741 agttaagata gcttttgaag ctacaccata tggaagtata catgaattta tatttaagtt
    31801 tttacaaatg ccattattaa aattaggaaa tagtttaggt gctatggcaa tcgcttatat
    31861 attcttacat ttattctggt tctttggaat aaatggaagt agtgttgttg gggctgtgta
    31921 taatccaata ttaaaaattc tatcagctga aaacttagct gcttttcaag ctggttcaaa
    31981 aataccaaac ataataacag gtcaattcca agatatgttt gcgacatttg gtggagcagg
    32041 tagtacacta tcattagtca tagctatgtt attaatatgt aaaagtaaac gtataaagtc
    32101 aatgggaaaa ctatcaattt taccaggaat atttggtata aatgagccat tgatatttgg
    32161 tctaccaata atgcttaacc caatactatt gataccattt gcaatagttc caacaataaa
    32221 tataataata gcttacttct gtatgtcagc aggattagtg cctcttacaa atggagttca
    32281 gttaccgtgg acaactccaa ttatattctc tggtttttta acaacaggat ggcaaggtgc
    32341 agtcttacaa ttagtacttc ttatactagg aatatttatg tatataccat ttataaaaat
    32401 gctagataag caatacttga gagaagaaaa tgaaactgtt gaagaggaag atgatgatat
    32461 atcttttgat gatttagtat tataggagga agattatgaa gaaaatagtt atgatattgg
    32521 accaaataca agctggagct ggaggaaaag agaaaagtaa tatacctcct gctggaaaat
    32581 caagcccatt aggaccaggt gtaatgatgg agcaattttt aaatgaatct aaggtaatag
    32641 ctaccttatt ttgtggggat gaattttttg taaataatca agaagaagta acgagtaaga
    32701 tgatagctat ggtaaaaaaa ttaaaccctg atgtagtaat ctgtggacca agctttaatt
    32761 acgaaaactt ttctaagatg tcagcaatac tgtctaaaaa tataaatgat aaaacagata
    32821 taccagcttt tgcagctatg tcagaagaaa atatagatgt gataaacgaa tataaaaatg
    32881 acatatgtat agttaagact ccaaaaaaag gtggaattgg tcttaatgat tcattaaata
    32941 acatatgtaa attggcaaaa gcaatagcaa ataaagaaga tataactttg atgaaagaag
    33001 agttttgtta tcaataagag tattaattat tagcaagttt aaatattata ctatttataa
    33061 taagcataca ttagtaaaat ctatataatt taaggagggg aattttaaca tgtggggaat
    33121 gatagcgaca tggagaatgg ccctagaagg aataactgag gcatcagaac atttagaaaa
    33181 aggcatggaa gcaggtgatg ctgtagagct tgctgtaaga agagttgaag actatccatt
    33241 ctataaatcg gttggatatg gtggacttcc aaatgaaaat tgtgaggtag aacttgacgc
    33301 tgcatttatg gatggtaaca cattagatat tggcgcagtt gctggtataa gagattattc
    33361 taatcctgtc agcatagcta ggaagttaag ttatgagaag gtaaataatt ttttagttgg
    33421 aataggtgct gaagattatg ctcataaaaa tggatttgaa agaaaaaata tgttgactga
    33481 tagagcgaag ttacattata aaaagagaaa aaaagagact ttagacaaag gattaagccc
    33541 atatgctggt catgatactg ttggtatgat atctttagac aaaactggaa aaatgtgtgc
    33601 agctacatca acaagtggat tgtttatgaa gaaaagaggt agaataggag attctgcact
    33661 atctggttca ggattctatg ttgatagtag tataggagga gctacagcta ctggtttggg
    33721 agaagattta atgaagggat gtatatctta tgagatagtt agacttatga aagaagggaa
    33781 gcatccacaa gaagcatgtg atattgcagt aaatgaactt gataaacagc ttatcaaaag
    33841 aagaggaaaa gctggtgatt tatcattagt tgccatgaat aataaaggtg aatggggagt
    33901 agctacaaat atagctgatt ttactttttc agtagtaact aaagaagaat ctcctactgt
    33961 atatatttca aatagagtag atggaaagac tatacataag gttatatcaa atgagtggat
    34021 ggaagcatat gaggctagaa taaaagcacc aatagaagta agtgagtaaa gtagtggtta
    34081 cataattaaa atagagtttt ataataagga gatttttatg aatatcataa gtaataaaat
    34141 agatgagtta aaagaagatt tattaagtga tattatagat attgtaaaaa tacctagtgt
    34201 caaaggggag tcagaaaatg gcttcccttt tggcgaaaaa gtaggggagg cacttaataa
    34261 agcattagaa atatcagaaa aattgggatt taaggttaga aacttggata attatattgg
    34321 ttatgcagag catggagata gtgatgatta tgtttgtgta attggacatg tagatgttgt
    34381 acatgaagga gatggttgga agcatcagcc ttataaggga gaagaaacta atggaagaat
    34441 ttatggacga ggagtactag acaataaagg acctataatg tctgccttat atggattata
    34501 tgctattaaa gaattaaact taaaattaga taagagagtt agaataatat ttggtacaaa
    34561 tgaagaaagt ggatttgaag atataccata ttatttagaa aaagaaaaag cacctattat
    34621 gggatttaca ccagattgta aatatcctgt tgtatatggt gaaaaaggca tggcaaagat
    34681 tagaataaaa agtaaaatca attatgaaga agatgtatac ctaggtttta tagaaaatat
    34741 gtctgaaaat gtgcttgtta catataaaga gctgaatata gaaaatagtg atactatatt
    34801 agatataaag gtaaaatacg attttagtta taaattgaaa gatgtattgg atgaaataaa
    34861 agctagtttt ccaaatagta tcgatataga ggtaatttct aattttaatc ctgtatattt
    34921 tgataaagag agtaatcttg ttaaaaagct tcagctagct tatgaaaggg ttacatcatt
    34981 agatggaact cccgtaacaa ctaatggagg tacgtatgca aaagtaatgc caaatatagt
    35041 tccgtttgga ccttcatttc caggacaaaa aggaatagct cataatcctg atgaatacat
    35101 ggatatagaa gatataatat taaatgcaaa gatatttgca aatgcaatat atgaattagc
    35161 aaaggagtag ttatgttaga aataatagga atgtcagtag aagacgctaa aattatagaa
    35221 gattgtggtg cagatagaat agagttagtt agtgctttaa ctgaaggtgg attgacacca
    35281 agttttggtc ttatagaaag tgttgttaac agtgtaaaga tacctgtgaa cgtaatgata
    35341 agacatcatg cgaaaagttt tgtatacagt aaagaagata taagtataat gcaaaaagat
    35401 ataagtgtgg ttaaagagat tggtgctaat ggagttgtat ttggtgtatt ggataaaaac
    35461 aatagtatag atgagaaaaa tctaaatgtt ttattgaaat gttgtgataa tcttgatgtt
    35521 acatttcata gagcaataga tgaatcaaat acaatagata gtgttaaaat tttgaaggat
    35581 tatgataaaa ttacgaatat attaacatca ggagggaaag gaagtatagt tgataacata
    35641 caaatgataa agaatatgat gcttagttct aatcacataa aaattctttt aggtggaggt
    35701 cttaatttta ataatataga aaagataaag gaattaacta aggcaagtaa ttttcatttt
    35761 ggtacagcga ttagaataaa taacagtcct tttgaagata tagatagaca aaagcttaaa
    35821 caactcgtaa atataatttc aagatagtat atgaatataa aatacttagt ttaaataaag
    35881 agagtaagtt aattataatt tgggtatatg aatgttaata ggtattaaat atgaaattat
    35941 tgttgagtat gctttataac ttttaattag ttttaaatat aaataatgaa agaaaagata
    36001 gattatggag gttttagagg gtttatattt attaaaaaat tataaaggta tattggcata
    36061 taatacacat aatttcctaa aattaccatc ttatgacatg aattcacata ttaataaaca
    36121 attaatggag atttttgtgg atagaaatag gagtaaaaat agctttaaat aaacaaaaac
    36181 aagagttatt aacagtttta tccacattat ccacaggtgt ttgtaataat attaatccac
    36241 ataagtatca atgtaactaa aaatgataaa taattctatc aattgaatag aaaaaatcat
    36301 aaaagtaaat aataaaaaga ggattatgag agttcgtgta gaatatatta ttaaagtata
    36361 aattttctta tactggcata attgaaggga gatgtattta tgaacataat tatatctgga
    36421 aaacaaatcg aattaactga tgcaataaaa aataaaatcg aaactaaact aagtaagtta
    36481 gataattaca ttcatccaga cactgatgta aaagttacag taagtgcgag aaaagcaaga
    36541 caaaaaatag aagtaacaat agctactgta aatggtccta taattagagc agaagatatg
    36601 caggaaaatt tatacacagc tatagatgtt gtatatgata aattaagtaa acaattaaaa
    36661 aaatataaaa agagattaca agataaacat aaagacaaca agagtataag attccaagat
    36721 gcagagataa atctagctga tgaacaagag aattatgggg atgaaaatca agaattagta
    36781 attcaaagac gtaagaaatt tagtgttaag ccaatgagtg aagaagaagc tgtattacag
    36841 atggaattaa taggacatga tttttatatg ttcaagaaca tagattcaga tgaaatagca
    36901 gtagtttata agcgtaataa tggtggatat ggtataatag aacatgaata aagcaattta
    36961 gatttaatca tattagctat cgataaataa attatcgata gctttttaaa ttaatattaa
    37021 aagtatagtt tttatggtaa aatgaataat agtacaataa agaacgagga gagatgtaaa
    37081 atatgagctt tatggataat ttattcaaca tggcagataa aaaagaatta aaaaagttca
    37141 ataaaactgt tgatataata gattcattag aacctaaatt tgaatctatg gcagactcgg
    37201 aacttaaaaa tatgacaaat atatttaaag aaagattagc aaatggtgaa agtatagatg
    37261 atatacttcc agaagctttt gcagtagtta gagaagtttc taagagagtg ttaggactta
    37321 gacactacag agttcaaatg attggaggta tagtactaca tcaaggaaga atagcagaaa
    37381 tgaaaacagg tgaaggtaaa actttggttg caactgctcc tgtttatttg aatgcgctta
    37441 ctggaaaagg tgtacatgtt gtaacagtaa atgattacct tgcaaaacgt gatagagacc
    37501 aaatggcaaa aatctatgag tttttaggaa tgagtgtagg cgttattata cacggtcaaa
    37561 atccaaaagt tagaaaagaa caatatgatt gtgatataac ttatggtaca aataatgaat
    37621 atggatttga ctatctaaag gacaatatgg taatacacaa agagcaaaga gttcaaagag
    37681 gattaaatta tgctatagtg gatgaggttg actctatact aatagatgag gctagaacac
    37741 cacttatcat atctggacct ggagacaaat caactcactt atattcagat gcaaacacat
    37801 ttgttttaac cttaaaacca gatgattatg aattagaaga aaaagataag gctgtatctc
    37861 taacagcatc gggtatacaa aaagcagaag tgtattttaa tgttgataat ataacagata
    37921 tatctcatac agaattatac catcatataa atcaagcttt aagagcacat gtaataatga
    37981 agaaagatgt tgattatgtt gctaaagatg gagaaatagt aatagtagat gagttcacag
    38041 gtagacttat gtttggtaga agatattcag aaggtttaca tcaagcaata gaggctaaag
    38101 aaggtcttaa aatacaaaga gaatctaaaa cactagctac tgtaacattc caaaattact
    38161 ttagaatgta caaaaaactt tctgggatga ctggtacagc aaaaacagaa gaagaagagt
    38221 ttaaagcaat ctacaaaatg gatgttttcc aagtacctac taataagctt atgataaggg
    38281 aagacttacc agactgtgta tacaaaagtg aaataggtaa atttaatgct gtagcacaag
    38341 aaataattga aaggcacaaa gtcaatcaac caatacttgt aggtacagta tcaatagaaa
    38401 aatcagaatt attatcacaa atactaaaga aaaagggcat aaagcacgag gtattaaatg
    38461 ctaagcatca tgataaagag gcagaaataa tagcacaagc tggtagactt ggagctgtta
    38521 caatagctac taatatggct ggtcgtggta cagatatagt tctaggtgga aatccagact
    38581 ttttaactaa gagagaaatg agaagaaatg gatttaaaga agaaatagta aatagagtag
    38641 atactccaat agaaggtata cctgtaaaag gtaatgaaat tctatttgaa gctagagaag
    38701 agtatgaaaa actatttgaa aagtttaagc aacaaacaca agaagaacaa aaacaagttg
    38761 tagaagcagg tggacttgct ataataggta cagaaagaca cgaatctcga agaatagata
    38821 accagttgag aggtcgtgct ggacgtcaag gtgacccagg gagttcaaga ttctatatag
    38881 gtcttgatga tgaccttatg agattgtttg gtagtgatag aatatctggt attgtagata
    38941 aaataggtct tgaagaagac atgccgatag aacatagaat attatctaag tctattgaag
    39001 gagcacaaaa gaaagtcgaa ggtaaaaact ttggaataag aaagcatgta cttcaatatg
    39061 atgatgttat gaataaacaa agagaaataa tatatgcaga gagaaaacgt gtattagaag
    39121 gtgaagattt acaagaacaa atacaatcaa tgactcattc tataatagaa gaagcagtta
    39181 ctctttatac acaagataaa ggatttgatg aagaaggatt caaagaacat atgtataatc
    39241 tgttcttgcc aaaaggtagc atagaaatac cagaaataga aaaacttaat ccagttgaaa
    39301 taacagaaaa agtttatgaa atagctatga agatatatac ttcaaaagaa gaacaagttg
    39361 gctatgagag aatgagagaa gttgagagag taatcttact tcaagctgtt gataaccatt
    39421 ggatagacca tatagatgct atggaccaat tacgccaagg tattggtctt cgtgctgtag
    39481 gtcagcaaga cccagttatt gcttataaaa tggaaggttt tgatatgttt gatgaaatga
    39541 ataaacatat caaagaagat actgtaagat atttatttaa tataacaata gaaactccag
    39601 tggaaagaaa agcagttgta gatgttgaaa atctatcttc tccatcagat ggaacacttc
    39661 caacaagtaa aactgttaaa aaagatgaaa aggttggaag aaatgactta tgcccttgtg
    39721 gaagtggtaa aaaatataaa aactgttgtg gaagataatc tcggaggatt aaatgttaaa
    39781 gttacaagag tatagacata gtatggagca gatggcttca aatttagaag aattgagggt
    39841 ttctctttga catagcttca ttgatgaaaa atatacaaat taatgaagaa aagatgaatc
    39901 aacaagattt ttggaatgat aatgaagtcg ctcaaagagt acttcaagaa aataaatctt
    39961 taaaagagac attagaagaa tatgaaagct taaaaagctt attagaagat atagaagtat
    40021 taattgaaat aggtttagaa gaagatgatg attctgtaga aagagatata gaaaagtcca
    40081 tagaatcaat ggaagaaaaa ttatctgaaa tgaaaataaa gactcttcta aatggagagt
    40141 atgataaaaa taatgctata ttatctataa atgctggtac tggtggctta gatgctcaag
    40201 attgggctca aatgcttttg agaatgtata taagatggtc agaaagtaag ggttataaag
    40261 ttaagctttt agatataata tcagaccctg aagcagggat aaagacagct acaattcttg
    40321 tagaaggaac aaatgcttat gggtatttaa agagtgaaaa aggagttcat agattagtga
    40381 gaatatctcc atttgaccca tcaggaaaaa gacatacttc atttgcatct atagatgtaa
    40441 caccagagtt agatgaaaat atagaagtag aaattaatcc atcagattta aagatagaca
    40501 catatagagc ttctggagct ggagggcaac atgtaaatac gactgattca gcagttagaa
    40561 taacacatat ccctactggt gtagtagttc agtgccaaaa tgaaaggtct cagcatctaa
    40621 ataaggacag agctatgaga cttttaatgg cgaaattgat agaattaaaa gagttagagc
    40681 aaaaggaaaa aatagaagat atacaaggca aatactcaca aataacttgg ggtagtcaaa
    40741 ttaggtcata tgtttttcaa ccatataaat tggttaaaga ccatagaaca aatgctgagt
    40801 ttggaaatgt agatagtgta atgaatggaa atatagactt gtttattaat gaatacttaa
    40861 aaatgaataa aatagtttaa taaatatgca ctgtaattaa gatatatgta taagaaggtt
    40921 agtgcttaat atgcacttac agcacttagg ctgaggacga taaccctcag ccttcgttct
    40981 gttttgttat acttatgaaa aactaacatt aggagtgaat aaattggata taaatcaaat
    41041 cttaaaaaaa gagtttaatc ttagagatga acaaataaat aatacattaa aacttattga
    41101 tgaaggaaat acaataccat ttatagctag atatagaaaa gaaatgactg gcgaaatgag
    41161 tgatgttaca ctaagagaat tttatgaaaa attaatgtat ttgagaaatc ttcaaagtag
    41221 aaaagatgat gtagttagac taatagatga acaagggaaa ttaacagatg aaataacaca
    41281 aaatatagaa aaagcaaaaa cattacaaga agtagaagat atatatgctc catataaaca
    41341 aaagaaaaga actagagcaa caatagcaaa agaaaaagga ttagaaaatt tagcattgtc
    41401 aatactagag aataatttag ataatataga aatagaggca aagaattatc ttgatgaaga
    41461 gaaagaagtt ctttctattg aagatgctct aaaaggtgct agagatataa ttgcagaact
    41521 agtctcagat gatgctaaga ttagaaagta tataagagaa ttagctttaa gagaaggtat
    41581 gatagttagc aaaagtgcta ctgatgagaa atcggtttat gatatgtatt atgattatag
    41641 cgaagcagta aaaagtatgg ctccacatag agttttggca ataaacagag gagaaaaaga
    41701 aagcttccta aaggttaagt tggaaataaa taatgataaa gttttaaatt acataataaa
    41761 cgaatatgta aatgataaaa atttcaaaaa taaagaagaa atcgtaagtt caatagaaga
    41821 ttcttataaa agattaattt tcccatcaat agaaagagaa ataagaaatc atttaacaga
    41881 aatagctcaa gaaagagcta taagtgtatt tggaaagaat gttaaaagtt tattgcttca
    41941 accaccagtt aaagacaaag tagttatggg atttgaccct gcatttagaa ctggatgtaa
    42001 aatagctgta gtagataaaa atggtaaact actagattat acaacagtat atccaacaga
    42061 ccctcaaaat gatgtagagg gagcaaagaa agttttaaaa ggattgattg aaaaatacga
    42121 tatagatata atttcaattg gaaatggtac tgcatctaga gaatcagaaa cttttgtatc
    42181 agaaatgata aaagaaatag atagcgaagt gcagtatgtg atagttagtg aagcaggagc
    42241 atctgtttat tcagcatctg aacttgcaaa tgaggaacat ccagatataa atgtatcaat
    42301 aagaggagct atatctatag ctagaagatt acaagaccca ttagcagagc ttgtaaaaat
    42361 agaccctaaa agtattggtg ttggtcaata tcagcatgat ttaaacaaaa agagattaga
    42421 agaagtacta gatggagttg ttgaagattc tgtaaatagt gttggagtag atttaaatac
    42481 tgcatcatac tccctactag aacatgttgc aggaataagt aaagctatag caaaaaatat
    42541 aatagcatat agagaagaaa atggagattt tacgtcaaga gcacaattga aaaaagtaaa
    42601 aagactagga cctcaagcat ttactcaatg tgctggattt atgagaatct tagagggtaa
    42661 aaatccacta gataatacag gtgtgcatcc tgaaagttat gatatatgta aaaaaatgat
    42721 tgagataatt ggatattctt tagatgatgt aaagaataag aatataggtg aaatagatga
    42781 aaaaataaaa gaaattggct tgagggaatt aagtgaaaaa ctggaagtag gacaagttac
    42841 gttaaaagat atcatagctg aaatcaaaaa accaggaaga gacccaagag aagaaggtat
    42901 aaaacctata cttagaactg atgtcttaaa aattgaagat atacaagagg gtatgacttt
    42961 aaaaggtaca ataagaaatg ttgtagactt tggtgcattt gtagatattg gaataaaaaa
    43021 tgacggatta gttcataaat cagaaatgag taatagtttt gtgaaagacc ctatgagtat
    43081 tgttacagta ggtgatatag tagatgtaaa agtcattgga attgacttaa acaaaaaaag
    43141 agttgcctta tcaatgaaaa aatagataag tttatgtatg gttatagtac ttagattcta
    43201 tatttgtaaa taagattact cgagtgtata aatgttaaaa atttgtaaaa gtatatagta
    43261 agaaaatata ttaaaataag aacgatagtc ttgaaaatca ggaggaaaat gaatgatttt
    43321 tataaaaaat ggtaaaataa ataccataac aaatgggata attcatggag atatacttat
    43381 agatgaagga aaaataatag aaattggaga agatttgata gctcctttag atgtagaggt
    43441 aatagatgct agtaataaac ttgtgtttcc tggatttata gatgctcata ctcacttagg
    43501 tttatgggaa gatggtatag ggtttgaagg agcagatgga aatgaagaga ctgacccaat
    43561 aacaccacaa ttaaatccta tagatggaat taatccaatg gatagaactt ttaaagaagc
    43621 ttttgaaggt ggaataactt ctgtttgtac aacccctgga agtgcaaatg ttatgggtgg
    43681 tcaatgtata gctataaaga catgtggaaa aagaattgat aaaatggtaa taaaaaatcc
    43741 agttgcatct aaaattgctt ttggagaaaa tccaaagagc tgttatggtc aggatgataa
    43801 atctcctcaa actagaatgg ctatagctgc tttacttaga gaaaacctaa aaaaagcaga
    43861 agaatattta gaggatattg atatgtatga gagtcatgat gatgaagact gtgaaaaacc
    43921 tgaatatgat ataaaaatgg aaagtttaat accagtttta agaagagaaa ttccttttaa
    43981 ggcacatgct catagagcag atgatatgtt tacagcaata agaattgcaa aagaatttaa
    44041 tttaaagctt acattagacc attgtacaga aggtcatctt attgtagatg aattagtgga
    44101 agaagggttt cctgtaatag tagggccatc tttgtcagaa aggtctaaat ttgagcttag
    44161 aaatttgaca tttaatacag caggaatact ttcaaatgca ggtcttgatg tatgtataat
    44221 gacagaccat ccagtaatac cagttcaata tcttccaata tgtgcaggta ttgcagtaaa
    44281 acatggtatg aaagaagaaa aagcaataga atcaattacg ataaatccag ctaaggcttt
    44341 aggaatagaa gatagagtag gctctataga agttggtaaa gatgcagacc tagtaatttg
    44401 ggataattct ccactagaga ttcaaagtaa tgtactttac actataataa atggcaaagt
    44461 agtgtatgag aaaaaataac ttgacaatta ttaaaaaatt aactaatata agagtgtaag
    44521 gaaaattaaa aaatcaaaga agatcttcgg ggcagggtga aattccctac cggcggtata
    44581 gcccgcgaac tacttaggtg gttgatttgg tgagattcca aagccgacag taaagtctgg
    44641 atgagagaag atatttatgg aagataaata agtttatttt agtttgttta aactatatcc
    44701 ataaaaactc gaagataggg tcttcgagtt ttttgttttt cctaaaaaat ataaacatat
    44761 tgattagggg gaaaataatt atgcaaaaca cagcaaaaag aggcaatatg atttcaacaa
    44821 gaactttagt agtaatgtca attttatctg caatatctta tttattaatg tttattcaag
    44881 caccaatacc aggtatattt ccagatttct taaaaattga cttatcagat attccagcaa
    44941 tattcggagg aatgtctcta ggaccattag ttggatttgg aatagttgta gtaaaaaatg
    45001 tgttacaagc aataacagca acaacaactg gaggaatagg agaatttgca aatatattaa
    45061 ttggtggttc ttatgtaatg atactttgtt attcatataa gagaagtgga gatttaaaag
    45121 gtgtactgat tggttttgta ttaggaacaa tatctatgac tataattggt tcattagtaa
    45181 attattttgt aatgttgcca ctttatggtc aacttatggg actagatgct ataataggtt
    45241 taggttcagc tataaatcct caagttcatg atttattcac ttttgtaatt tggatgatag
    45301 ctccatttaa tgtgctgaag gctgttataa tatctttagt aacattgcca ctttataaaa
    45361 aaatgggaaa tataataaag aaataattag acaagaaaat ataaaattaa atgtattgaa
    45421 tagccataat ttatttataa cttcagtcct atattgtgat tgatatatta aatatattat
    45481 ggctttatta atttatcaaa aaatgtatat aaaataagaa atatttagac aaatttaaat
    45541 tttaaaataa aggaagaatt aaaacgatta actattaaaa tatgaaaaag gtatctacaa
    45601 tatacagagc gaatttataa taaacaaaaa ataaagttat tattaaaaaa ctataaagat
    45661 taataatcaa acttttaata ataactaatt taataatgat aggggtgtat attcatgcat
    45721 ttatctaaaa aaataaaaaa attattttta gatgataaag ggtattattt taatagtaat
    45781 tggaaagata ttcatattgg tatttatgat acaaaattag gagattcttt aaagtatatc
    45841 actaatgaat atattgaaaa tatggaaagg gtaaaatacg attatcgtga attaaaaaat
    45901 atagaaaatc aaactgatgg tatgtgtatt ttagcaataa ggcagaatta caaggcatta
    45961 gagtatgtaa aaaatcaaac cgaagaatta tgtttagaag caataaagta taattataaa
    46021 gcacttgaat atgtaaaaga acaaacagaa tacttgtgtc ttgaagctat aaaaaaagat
    46081 tgtaatgcac ttaagtatgt acgaaataaa acagaagggc ttattataaa agcaataagt
    46141 cattcttcca atatagatgt tgtaagtata ctaaaggctt tagagactca aacaagaagg
    46201 atatgtttag aagcaattaa aaaagatggc aggtgtcttg catacgttag agaacagagt
    46261 gaagaattat gtatagaggc cattaagcaa aattataaag cattaaaata tgtaaaaaat
    46321 caaacggaaa aaatgtgtat agaatccgta agacaaaatg gtatggcatt acaatatgta
    46381 aataaacaaa ctgataaaat atgtatagaa gctgtcaaac aagatggaag aagtatacaa
    46441 tttgtcaaca ataagacaga agaaatatgt ataaatgcta taagatattt aaataaaaaa
    46501 tataatatta aagatgtact aagttacata gataagtata cagaagatat atgtatagag
    46561 attgtaagac aaaatggaaa aatgcttatg tatataaaaa atcaaacaga aaaaatgtgt
    46621 attgaagcag taaaagagaa ctataagtct cttaagtatg taaaagagca atctgagaga
    46681 atatgtaaag aagcattgaa gcaaaaccat aaagcaaaag agtatgtaaa aattgctatt
    46741 gatgattgta tataaggtgt agatacagcc atcaatagtg aaagataaaa aaagataaag
    46801 acaagtactt tataataatc taggcttata ctataattaa ttaagcagtg atataaattc
    46861 acatgacgat tatatcaaaa tggagatgaa ttgatggcaa aaatatattt agaaaatgaa
    46921 aataaaacta gagaaatagg atataaatta ggtaaacttt taaaagaagg cagtgttatc
    46981 tgtttagtag gagatttagg agctggtaaa actacaatga cacaaagttt ggcagattca
    47041 ttagggatag aagattatat aacaagtcct acttttacta taataaatga atatgaagga
    47101 aaaatacctt tgtatcattt tgatgtttat agaataggta gtagtgatga aatgtatgat
    47161 attggttatg atgaatatgt aaattcaaat ggaatctgta taatagaatg ggctaatcta
    47221 atagaagata tacttccaaa agaatatttg aatatagaat tgagatataa agatgaagga
    47281 agagaaatga tattaactcc aaaaggagag ttttataaag agatagttga ggagttgatt
    47341 aaataatgaa aatactaggt atggatacgt cttctatggc agcaagtgta gcagttgttg
    47401 aagatgataa cttaatatgt gagtttacag taaacaacaa aaaaactcat tcacaaaagc
    47461 ttatgccaat gatagaaaat atgttgagta tgagtgattt aagtataaaa gatatggatt
    47521 tattagctgt atgtataggt ccaggttcgt ttacaggact tagaataggt atggctacag
    47581 taaaagctat ggcccatgta aataatatac caataatagc agtaaattct ttggaatctc
    47641 tagcaaataa cataaatttc tgtgatagaa agatatgttg tatattagat gcacagaaaa
    47701 atcaagttta ttcagctaaa tacaagtttg aaaatggaga gatgatagaa ttagatggtg
    47761 ttgatgttgt agagtttgat gctttagtag atgaaattgt atcaactaat gaagaattta
    47821 taatagttgg agaagctgta tacaaatata aagacaagtt agagaatata aaaaatataa
    47881 aaataccttc accagctaat aatgtatcta acgcaggaag cttatgctct ttagcattaa
    47941 ataaatataa taaaaatata gatgtacata cttgttatac gataaatcct atgtatatca
    48001 gaaaatctca agctgaagta caatatgatg aaaaaatgaa gaggctaaat aatggaagat
    48061 aatacaaaac taatagtaga tgatataaaa atagaagaaa tgactacaga agatatagat
    48121 gaagtttttg aagtagaaaa aaattgtttt gaagattatt ggtcaaaaga gtcttttaga
    48181 aaagagttgt ctaatgaagt agcaaggtat atagtagcta agttaaatgg aaaaattgtc
    48241 ggatatgtgg gaatttggct tatacttgat gaagggcata taaataatgt ggctgttcat
    48301 agtgactata gagggaaaaa aataggggac aagttgataa aaggcatagt tgatttatgt
    48361 aaagataata atattgcatc tatgacatta gaagttagag catcaaataa aatagcacaa
    48421 aatttatata gaaaatatgg atttaaaatg ggtggaataa gaaaagaata ctacagtgat
    48481 aataaagaag atgcaattat aatgtggaac caattaaaag aggtgtagag tttatgagtg
    48541 atataataac attagctata gagagtagtt gtgacgaaac agctgcatca gttttaaaaa
    48601 atggtagaga agtactttca aacataatct ctactcaaat cgagacacat aaaaaatttg
    48661 gtggtgtagt tccagaagtg gcctcaagaa aacatgtgga aaatattgat atagttgttc
    48721 aagaagcttt agacaaagca aatataggtt ttaatgatat agaccatatc gcagttacat
    48781 atggacctgg gctagtaggt gctttattgg ttggtctatc ttatgctaaa gcacttgcat
    48841 atactttgaa tattccactt gtaggagtaa atcatataga agggcatcta agtgcaaact
    48901 atatagagca taaggattta aaacctcctt ttataacatt aatagtatct ggaggtcata
    48961 ctcacttggt tgaagtaaaa gattatggca agtatgaaat tttaggaaaa acaagagatg
    49021 atgcatctgg agaagcattt gataagattt caagagctat gaatttaggc tatccaggag
    49081 gtcctattat tgataatctg gccaagaatg gaaataaaca tgctatagaa tttccaagag
    49141 cctatttaga agaagatagc tatgatttta gttttagtgg attaaaatct tctgtattaa
    49201 attatctaaa tggaaaaaga atgaaaaatg aagaaatagt agttgaagat gtagcagcat
    49261 ctttccaaga ggctgtagta gaggttttat ctactaaagc actaaaagca gtaaaagaca
    49321 aaggatacaa tatcataact ctttctggtg gggttgcttc taattcgggg cttagagcta
    49381 aaataacaga gcttgctaaa gacaatggta ttactgttaa gtatcctcct ctaattttat
    49441 gcacagataa tgcagctatg atagggtgtg caggatatta taattttata aatgggaaaa
    49501 cgcatgatat gagtttaaat gcagttccaa atttaaagat aaatcagtag agttagatga
    49561 atttgtaaat ataaaagtat tgtttcaaaa aagtaagtag aagtgaaatt aaatataata
    49621 aaaataaata tagtatatat taaaacttga agtaaaacta tttataatct ttatattcca
    49681 atgaattgca acaaatttta ggatatacag attgtctttg gcttagattt gcttcaagtt
    49741 tatttttttc tatagaaaga ttattttaaa aagtgggtaa tatacccttt tttttcattc
    49801 ttaaaaagtt ttatactaat tatagaaaga ttaaggaggg gaagaaatga gtcaaagtaa
    49861 agaagacaaa ataagaagta ttttggaagc aaaaaatatt aaatcaaatt ttcaaaataa
    49921 agaaaattta agtgaattta atgaaaaaaa agcaagtaaa agagcagaag acttattaga
    49981 tgtatattat aatacattat ctactgctga tatggaattt ccatattggt ataatagaga
    50041 atatagaaaa tcagatggtg atataccagt agttagaaga gctaaagctc ttaaagctgc
    50101 tttttcacat atgacaccaa atataatacc tggtgagaaa atagtaatgc aaaagacacg
    50161 ccattataga ggttcttttc caatgccatg ggtaagtgaa agcttctttg ttgctcaagg
    50221 agaacaaatg agagaagagg caaagaaatt agccagtaac acagctgatg aacttacaaa
    50281 atttgggtct ggtggaggaa atgttactga aagttttgga aatgtcgttt caatagctgg
    50341 taaattcgga atgagaaaag aagaagtacc tgtacttgta aaaatggcaa aggaatgggt
    50401 tggaaagtct gttgaagatt taggttttca ctatgaaaaa atgatgccag attatgattt
    50461 aaaagaaaac ctaatgagta cacttatatg catgtttgat tcagggtata cacttcctca
    50521 aggtagagaa gttataaatt atttttatcc tttaaattat ggattagatg gaattataga
    50581 aatggcaaaa gaatgtaaaa aggcagttgc aggaaatgca tctggagatg ggcttattgg
    50641 tatggaccgt ctatattttt atgaggctgt tattcaagtt attgaaggat tacaaacttg
    50701 gattttaaat tatgcaaaac atgcaaaata tttagaaagt atagagacag atttagaagc
    50761 taaaaaagaa tactcagatt tagtggagat attagaacat attgcacata aacaacctcg
    50821 tacatttagg gaagctcttc agttgactta tacaattcat atagcatcag taaatgagga
    50881 tgctatatct ggtatgtcta taggacgttt tggtcaaatt ttatatcctt ggtatgaaca
    50941 agatatagaa aaaggactaa ttacaaaaga agaggttatc gaattattag agctttatag
    51001 aataaaaatc acttgtattg actgttttgc ttcagcgggc gtaaatggag gagtactatc
    51061 aggcaataca tttaatactt tatctatagg tggattaaaa gaagatggtt ctacaggagc
    51121 taatgaacta gaagagttat tattagaagc aagtatgaga tgtagaactc ctcaacctag
    51181 tttaactatg ctatatgatg aaaagcttcc agaagatttc ttaatgaaag ctgctgaatg
    51241 tacaaagctt ggttctggtt atccagcatg ggtaaacaac tcaaatggaa caacatttat
    51301 gatgaaacaa tttgctgatg aaggtatgac tgttgaagaa gcaagagcat ttgcattagg
    51361 tggatgtttg gaaacgtctc cagggtgttg gaaacagtta actttaaacg gaaaaacata
    51421 ctctattgca ggtggagcag gtcaatctgc tggctctgga gttcacttta tagctaatcc
    51481 aaaaatttta gaacttgttc ttatgaatgg taaagactat cgtatgaata tacaagtatt
    51541 tgagcctcat aataagccat tagatactta tgaagaagtc atagaagtat ttaaagatta
    51601 ttataaacaa gcaataaatg ttttagaaag agcaaataac attgagttag atatatggag
    51661 aaaatttgat acttctataa taaattcact actaaagcca gactgtttag acaaaggtca
    51721 acatatagga aatatgggat atcgttataa tgcaacatta aacgtagaaa cttgtggtac
    51781 tgttactatg gtcaattcat ttgcagcact taagaaactt gtatatgatg acaaagcatt
    51841 tacaatagag gaaatgaagg atgccatatt aaataatttt ggatttaaag atgcattaga
    51901 agtaggaaat tattctatgg cagaccaagt aaaagtagat aagactggta aatatgatgc
    51961 tatttataag gcctgtcttg atgcacctaa atatggaaat aatgatttgt atgcagataa
    52021 tattcttaaa aactatgagg tatggttatc aaaagtatgt gaagaagcac aatctttata
    52081 tgctaagaag atgtaccctt gccaaatatc tgtttctaca catgggccac agggagctgc
    52141 taccttggct actccagatg gtagactaag tggaacaaca tattcagatg gttcagtctc
    52201 tgcatatgca ggaacagata aaaatggagt atatgcatta tttgaatctg caactatttg
    52261 ggaccaagca gtagttcaaa attctcagat gaatttaaaa cttcatccaa ctactataaa
    52321 aggacaacaa ggaactaaaa aattattaga tttaacaaga agttatttaa gaaaaggtgg
    52381 gttccatatt caatataatg ttgtagattc agaaacatta aaagatgcac aaaaaaatcc
    52441 tgacaattat cgtcaattaa tggtacgtgt tgctgggttt actcaatatt ggtgtgaatt
    52501 aggtaaacca atacaagatg aagtaattgc tagaacagag tatgaagggg tgtaaaatat
    52561 gagaaagcat agtgattgta tgaatttttg tgcagtagat gcaaccaaag gaatttgtag
    52621 attatcaaaa caaatgatta atttagatga tgcagcatgt ccagagataa aagtaatgcc
    52681 aaaatgtaaa aattgtaaaa attttgttga agctaatgat gaaggtatag gcaaatgtgt
    52741 tggcctagag aaagaagact gggtatattc aacattgaat gcaattactt gtgaaggaca
    52801 tgtgtttaat gagtagtcaa aagcaattag aaggcatgat ttttgatgta caaagctttt
    52861 cagttcatga tggtccaggt tgtagaacaa ctgtattttt aaatggatgt ccactgagtt
    52921 gtaaatggtg tgcaaatcca gaaagttgga ctgtaagacc acatatgatg tttagtgaat
    52981 tatcttgtca atatgaaaat ggatgtactg tatgtcatgg taagtgtaaa aatggtgcat
    53041 tgagctttaa tcttgataat aagccagtta tagattggaa catctgtaag gactgtgaaa
    53101 gttttgaatg cgtcaactca tgttattata atgcgtttaa attatgtgca aaaccttaca
    53161 ctgtagatga acttgtgcag gtaattaaac gtgattctaa caattggaga agtaatggag
    53221 gagtaacatt tagtggaggt gagccactac ttcagcatga atttttacat gaagtattat
    53281 taaagtgtca tgaggtaaat atacacactg ccattgaaac gagtgcatgt gtatcaaatg
    53341 aagtttttaa taaaatattt aacgatattg attttgcatt tattgatata aagcatatgg
    53401 atagagaaaa acacaaagaa caaacaggtg tgtataatga tttaatatta gaaaatatat
    53461 caaaccttgc aaattctgat tggaatggaa gattagtact tagagtacca gttatctctg
    53521 ggtttaatga tagtgttgaa aatataagtg atattatatc ttttatgcac aaaaataatt
    53581 tggtagaaat taatctatta ccatttcaca gattaggaga atcaaaatgg attcaattag
    53641 gcaaggaata tgagtattct gataagggtg atgttgatga aggtcatctt gaagaattac
    53701 aagatatatt tttggataat ggtatagctt gttatgttgg tcatgagaca gctttctaaa
    53761 attaaataca agttttaatt aaaaaaagga tgttacacta aaagctagag ttacatcctt
    53821 ttttatgtgg tttttatgta gaaataatta agtaaagttt tatatgtttt atatattgtt
    53881 tatattgctt tatgtccata aatattgctt taaatatact ttatatattt tttatagttt
    53941 aaattacagt tagagaatat attacgctta cttcattgaa gcagaagttt tgtggtaaaa
    54001 tactactatg aaaaaattta atagaatata ttttagaaac aactaaaaat aatagttagg
    54061 aggaagacat ctatatgaac aaatcatgga gcaacttgat tattaagaat aaagacaaaa
    54121 aagtacgttc agggtggatt attatagctg ttatggctac cttttacata cttcagtatt
    54181 atttaagcat gatacttgtg gaaataataa gaaaaatcct ggttgctaca ggtgacatta
    54241 atttaaaaac aaactatttt tcagaatatg ctaattggtt aaatgatgtt gggttacaga
    54301 ttgtatttca aattctaaca gattcactta tgattattat tcctataatt gtatggagat
    54361 ttattatgaa aaatcctatt agtgaaatgg gattacattc ttatagaatt aagaaaaaag
    54421 aaggatatgt aggaatgtta ttaggaatat ttaattgtac agtaatcttt attataacaa
    54481 taacaattgg tggtggtcat gttacttcat gggtaccaaa aatttcacct ttaacagttt
    54541 cttggatact ggtctttatt ttagttgctt ttgcagaaga aattttaaat cgtggttttt
    54601 ttatggcagt tttacgaaga tgtaaaaata tttattttat aatgattgtt ccatctgtta
    54661 tttttgggtt gattcatata tggaatcctg atgttacttt tttgtctgta attaatatta
    54721 taattattgg gattcttttt tcatatatgt ttataaaatc gtcgaatata tggatgtgta
    54781 taggttatca ttttgcatgg aatgtatttc aggggattat atatggtatg cctgtaagtg
    54841 gacttcaagt tccagggatt ataactacac aaatcactca tggtaatcta ttaaatggag
    54901 gaatgtttgg tattgaaggt ggaattttga ctacttttgt taatctactt agctttatat
    54961 ttgtatggta ttactataga gattctaagt atgattttgt tagtgataaa gtaaatgtga
    55021 ccaatatcaa gaattagttg attataataa attttaataa tatcttctaa gcttaaagta
    55081 ttctgtagga ttgttggaaa tttgcaaaaa tataaaaata acttggatat tctatttact
    55141 ccaagttatt tttatattct tttgtaaata gaaatggaac ataaaaaatt aaaacaacca
    55201 tagatactat tagtatatca tggtaagtga tagttgattc tttagaattt gtgataagaa
    55261 aagcactatt atttaaaaca tgaattatag cacaaaacca gatattctta gtttttatat
    55321 aaagatatcc taaaaatatt gaaattccaa ctatatgtat tgaacgtaat attattccta
    55381 atattggcgc ttcaggtgta tataatgtaa atattagtgg taagtgccac aagctccaac
    55441 acatacctaa tattataact cctaactttt taccaaattt atcaaaaaaa atattctgta
    55501 aaaaacctct ccaggcatat tcttcactaa aaaaaatata aatagtagtt aatgatgtta
    55561 ttggagtaaa tataaatttt atgataccac tataattagg ttgagcccct tgtagtatta
    55621 gtataaatga tgttggaaca attacagaaa tcataaagta aataattaat aaagaaccta
    55681 gtttaatatt ttttacagga tataaatttt ttttatttga tgaatatatt ataattgata
    55741 agctacttat aaaagttaat at
//
LOCUS       DAMJYX010000016        51657 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJYX010000016 DAMJYX010000000
VERSION     DAMJYX010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.016"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            27..101
                     /locus_tag="PC479_001049"
     tRNA            27..101
                     /locus_tag="PC479_001049"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC479_001050"
     tRNA            116..200
                     /locus_tag="PC479_001050"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC479_001051"
     tRNA            210..293
                     /locus_tag="PC479_001051"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC479_001052"
     tRNA            317..391
                     /locus_tag="PC479_001052"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC479_001053"
     tRNA            416..492
                     /locus_tag="PC479_001053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC479_001054"
     tRNA            502..577
                     /locus_tag="PC479_001054"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC479_001055"
     tRNA            586..661
                     /locus_tag="PC479_001055"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC479_001056"
     tRNA            664..752
                     /locus_tag="PC479_001056"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC479_001057"
     tRNA            756..831
                     /locus_tag="PC479_001057"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC479_001058"
     tRNA            838..911
                     /locus_tag="PC479_001058"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC479_001059"
     tRNA            926..1002
                     /locus_tag="PC479_001059"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC479_001060"
     tRNA            1020..1096
                     /locus_tag="PC479_001060"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC479_001061"
     tRNA            1105..1180
                     /locus_tag="PC479_001061"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC479_001062"
     tRNA            1188..1261
                     /locus_tag="PC479_001062"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC479_001063"
     tRNA            1269..1345
                     /locus_tag="PC479_001063"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC479_001064"
     tRNA            1358..1433
                     /locus_tag="PC479_001064"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC479_001065"
     CDS             1509..1922
                     /locus_tag="PC479_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803619.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC479_001066"
     CDS             2231..5662
                     /locus_tag="PC479_001066"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2803620.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC479_001067"
     CDS             5904..7832
                     /locus_tag="PC479_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2803621.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            8285..8749
                     /locus_tag="PC479_001068"
     CDS             8285..8749
                     /locus_tag="PC479_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2803622.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            8756..9250
                     /locus_tag="PC479_001069"
     CDS             8756..9250
                     /locus_tag="PC479_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2803623.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            9266..10291
                     /locus_tag="PC479_001070"
     CDS             9266..10291
                     /locus_tag="PC479_001070"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2803624.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            10278..12725
                     /locus_tag="PC479_001071"
     CDS             10278..12725
                     /locus_tag="PC479_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2803625.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            12905..14278
                     /gene="radA"
                     /locus_tag="PC479_001072"
     CDS             12905..14278
                     /gene="radA"
                     /locus_tag="PC479_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2803626.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            14281..15351
                     /gene="disA"
                     /locus_tag="PC479_001073"
     CDS             14281..15351
                     /gene="disA"
                     /locus_tag="PC479_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2803627.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            15440..16534
                     /locus_tag="PC479_001074"
     CDS             15440..16534
                     /locus_tag="PC479_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2803628.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            16663..18840
                     /locus_tag="PC479_001075"
     CDS             16663..18840
                     /locus_tag="PC479_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803629.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(19020..19871)
                     /locus_tag="PC479_001076"
     CDS             complement(19020..19871)
                     /locus_tag="PC479_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2803630.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            20088..21254
                     /locus_tag="PC479_001077"
     CDS             20088..21254
                     /locus_tag="PC479_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2803631.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            21276..22922
                     /locus_tag="PC479_001078"
     CDS             21276..22922
                     /locus_tag="PC479_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2803632.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            23019..24392
                     /locus_tag="PC479_001079"
     CDS             23019..24392
                     /locus_tag="PC479_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2803633.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            24837..25790
                     /locus_tag="PC479_001080"
     CDS             24837..25790
                     /locus_tag="PC479_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2803634.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            25828..26790
                     /locus_tag="PC479_001081"
     CDS             25828..26790
                     /locus_tag="PC479_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2803635.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            26815..27801
                     /locus_tag="PC479_001082"
     CDS             26815..27801
                     /locus_tag="PC479_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2803636.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            27814..28860
                     /locus_tag="PC479_001083"
     CDS             27814..28860
                     /locus_tag="PC479_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2803637.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            29027..30757
                     /gene="lpdA"
                     /locus_tag="PC479_001084"
     CDS             29027..30757
                     /gene="lpdA"
                     /locus_tag="PC479_001084"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2803638.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            31175..34123
                     /locus_tag="PC479_001085"
     CDS             31175..34123
                     /locus_tag="PC479_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803639.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            34101..34565
                     /locus_tag="PC479_001086"
     CDS             34101..34565
                     /locus_tag="PC479_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2803640.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            34678..34980
                     /locus_tag="PC479_001087"
     CDS             34678..34980
                     /locus_tag="PC479_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803641.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            35042..36406
                     /locus_tag="PC479_001088"
     CDS             35042..36406
                     /locus_tag="PC479_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2803642.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            36441..36914
                     /locus_tag="PC479_001089"
     CDS             36441..36914
                     /locus_tag="PC479_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803643.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            37086..37736
                     /locus_tag="PC479_001090"
     CDS             37086..37736
                     /locus_tag="PC479_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2803644.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            37910..38842
                     /locus_tag="PC479_001091"
     CDS             37910..38842
                     /locus_tag="PC479_001091"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2803645.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            39116..39829
                     /gene="ispD"
                     /locus_tag="PC479_001092"
     CDS             39116..39829
                     /gene="ispD"
                     /locus_tag="PC479_001092"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2803646.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            39826..40311
                     /locus_tag="PC479_001093"
     CDS             39826..40311
                     /locus_tag="PC479_001093"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2803647.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    40406..40614
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            40678..42393
                     /locus_tag="PC479_001094"
     CDS             40678..42393
                     /locus_tag="PC479_001094"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2803648.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            42515..43960
                     /locus_tag="PC479_001095"
     CDS             42515..43960
                     /locus_tag="PC479_001095"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2803649.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            44398..45879
                     /locus_tag="PC479_001096"
     CDS             44398..45879
                     /locus_tag="PC479_001096"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2803650.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            46100..46255
                     /locus_tag="PC479_001097"
     CDS             46100..46255
                     /locus_tag="PC479_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803651.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            46400..47797
                     /locus_tag="PC479_001098"
     CDS             46400..47797
                     /locus_tag="PC479_001098"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2803652.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            47782..48195
                     /locus_tag="PC479_001099"
     CDS             47782..48195
                     /locus_tag="PC479_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2803653.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            48301..49059
                     /locus_tag="PC479_001100"
     CDS             48301..49059
                     /locus_tag="PC479_001100"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2803654.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            49072..49809
                     /gene="rlmB"
                     /locus_tag="PC479_001101"
     CDS             49072..49809
                     /gene="rlmB"
                     /locus_tag="PC479_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2803655.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            49812..50339
                     /locus_tag="PC479_001102"
     CDS             49812..50339
                     /locus_tag="PC479_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2803656.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            50404..51051
                     /gene="sigH"
                     /locus_tag="PC479_001103"
     CDS             50404..51051
                     /gene="sigH"
                     /locus_tag="PC479_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2803657.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            51125..>51657
                     /locus_tag="PC479_001104"
     CDS             51125..>51657
                     /locus_tag="PC479_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDF2803658.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT        21015 a         5559 c         9796 g        15287 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actgaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttgaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttca ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaagagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacattag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aaaatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca tgaagcaaaa
     8101 aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa tgaaagaata
     8161 aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc tttaaaaatt
     8221 taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt gtgtggtgat
     8281 attaatggca actatgacag atataattga aaaatttatt aaagatttga tggaagaaga
     8341 taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg caccatcaca
     8401 aataaattat gtcttaacaa caagatttac aatagataga ggatattaca ttgaaagtaa
     8461 aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag atgggcatat
     8521 tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag caaaagaatt
     8581 attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat taatattata
     8641 tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga aggtaaggtc
     8701 taatatttta aagaatataa taataggatt atttagtata gaggagtgat aatttatgct
     8761 atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag tcaatggaga
     8821 aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa tgaattttaa
     8881 cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc aacctaaaaa
     8941 agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg agtttaagaa
     9001 taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag taaatccaat
     9061 gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa aatcttttta
     9121 taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata gagctataaa
     9181 aaatgaagag tatgagttag cagctcaatt tagagataaa attaaatatt taaaaggttc
     9241 tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat caagagttag
     9301 gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag aatgtgccat
     9361 ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac aaaaagaaga
     9421 atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta tggttgaaga
     9481 acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag taaaaaaaga
     9541 taaaactata agtataatga taaatgaaga agaccatata aggatacaaa ctatatgtga
     9601 tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt tgttagaatc
     9661 aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc caactaatac
     9721 aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc aactaggcta
     9781 tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag ggatatatgg
     9841 agaacgaaca gaagcgttgg gaaatatata tcaaatatct aatcaactta cacttggaag
     9901 aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa tttcaaaaga
     9961 aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag ataaaatatt
    10021 tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg caatgagcca
    10081 tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag atttaagggc
    10141 tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt ataagagtga
    10201 tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt tggaaaaatt
    10261 aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa aaggcaattg
    10321 atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt gaacatatac
    10381 ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt aaagttggat
    10441 ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga gaagaaatct
    10501 cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca ggaatgtttg
    10561 caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca attattcaag
    10621 aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat aggactttag
    10681 cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa gcagagaata
    10741 gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat ggaagaaatc
    10801 ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa aaagaaatac
    10861 aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg ataggagacc
    10921 caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc ttaggaaatg
    10981 ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca ttattggctg
    11041 gagctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat gaagttgtta
    11101 aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg gctgggtcta
    11161 ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca agaggagaaa
    11221 tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa aaagattctg
    11281 ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag gactcaataa
    11341 aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa ataactgatg
    11401 atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga tatctgccag
    11461 ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa gaaaatacac
    11521 ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa gaaaaagaag
    11581 aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa caaggtctac
    11641 ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa cattcagatt
    11701 tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata ccagttaata
    11761 aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg cacaataaag
    11821 ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg tcaagagcag
    11881 gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca acaggagtag
    11941 gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa aatcaaataa
    12001 ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg ataggttcac
    12061 ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta agaagaaatc
    12121 catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta tttaatattc
    12181 tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact gtagatttta
    12241 agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt agacaaaaaa
    12301 ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat gaaaaaatga
    12361 aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta aacagaatag
    12421 atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata gtacttttaa
    12481 tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa atgagtgatg
    12541 aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca agacctctta
    12601 aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta aaaggtaatg
    12661 tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt tttgaaacta
    12721 aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata atctaaacaa
    12781 aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta tttaatcttt
    12841 acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta aggatgataa
    12901 aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg aaacagctaa
    12961 atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag aaatagacca
    13021 aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac cagtcagtat
    13081 aaactcaata gagagtaaag aagaagagcg gtttacaaca gatataaatg aattagatag
    13141 agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg accctggtat
    13201 aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag gaaaaactgt
    13261 tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta aaagattagg
    13321 tattaactca gaaaaattat atatatttgc tgaaaataat ttaagtataa ttgaatctta
    13381 tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg tcttcagtcc
    13441 agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt caaaattcat
    13501 gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta caaaagaagg
    13561 ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat attttgaagg
    13621 agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg gttcaactaa
    13681 tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata atccatctaa
    13741 aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat ctactgtaga
    13801 aggtacgagg ccaatgttac ttgaattgca agcattagtt tctcctacaa gttttggtat
    13861 accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat tagcagtatt
    13921 agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca tagttggagg
    13981 aataaagata aatgaaccct caattgattt agggatagcc atttctgtag catctagttt
    14041 tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac ttacaggaga
    14101 agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac ttggatttaa
    14161 aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag gcatagaaat
    14221 atggccagta gataacttaa gacaagctat aaatatagta cttgggagga atcaataaat
    14281 atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat atctcctgga
    14341 actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg attaattgta
    14401 attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat aaatgcagaa
    14461 tattcaccat catatctata tgaattagct aaaatggatg gagctatagt tttaagtggt
    14521 gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt tatagaaaca
    14581 tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac tggtgctata
    14641 gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa tgagaagtat
    14701 gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca aactctggaa
    14761 aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga gtttaatgat
    14821 ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt aatgagagtt
    14881 acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt agtaagtatg
    14941 caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt caaagattat
    15001 aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt aaattcagaa
    15061 gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt ttcagaaagt
    15121 atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca tagactacca
    15181 acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat tttagatgca
    15241 tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac atatataaaa
    15301 aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg atgaatttta
    15361 aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata atagataagt
    15421 ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta ttttacttgg
    15481 atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa tattaacatt
    15541 tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa tagccatttt
    15601 aggatatctt atagagccat gggttgtaaa taaagtaaaa gaaattgcta aaatagtgga
    15661 taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg gtcttatagt
    15721 aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa tagtaggtgg
    15781 aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag ttgcgcttaa
    15841 aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc ctattaagga
    15901 taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa aagtattaga
    15961 tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt ttatagaagg
    16021 aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag attcttcaga
    16081 tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa ttcagaaaga
    16141 attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag aagttgatag
    16201 taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg attataactt
    16261 aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt tagctaatgc
    16321 tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta aagaaggaaa
    16381 agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg ttgatggagg
    16441 aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc aaactcctgc
    16501 aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc tataatttgg
    16561 gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat gtttaatgta
    16621 ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa aaagattata
    16681 tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag ttttgcagat
    16741 gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag tatgagtaat
    16801 atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat tggcttgatg
    16861 aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat gggtgctggt
    16921 ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag taaggataga
    16981 aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa tttaactata
    17041 aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt agggtcatta
    17101 ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc agatataatt
    17161 gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga tgaatatgga
    17221 agaataccta atggaaatgt agatactata aataaaaaag acttatctat gccttatgga
    17281 atatcaactg attatgataa attgattgta gagactaaag aagcttacaa aaataatgat
    17341 gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc aaatttaaat
    17401 gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat atatttaaaa
    17461 aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc atttccaagt
    17521 gatttagatt ataaaaataa aagaagactt tcgccaataa taaaattaaa tggagaaggg
    17581 aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa tttagatgtt
    17641 ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg aagaaaatat
    17701 gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca aaagatagta
    17761 tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt tttcttgtca
    17821 tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa ttataaagaa
    17881 acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc actgtcattt
    17941 atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg aataattata
    18001 ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt aaaaaacatg
    18061 ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt tggctcatat
    18121 ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata ttatggagta
    18181 ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc aattttactt
    18241 acatataaaa atataccaaa atggtctata atagtttttt ctttggttat cttaattaca
    18301 agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg tgttgcattt
    18361 ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat cgtattatta
    18421 ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat tttgggaagt
    18481 aattcacatt taggcatgtt tgtaaaagaa atttacttta atggaccagg agaaataata
    18541 caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag tgcatgggta
    18601 aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca gattagatac
    18661 tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc atcaatagca
    18721 ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc tacagccttt
    18781 atttatgtaa ttgtacctat gataacatta agtataaatc ttacagcatt aaaagaataa
    18841 gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta tttacaaaat
    18901 aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc aaaattgatt
    18961 attctaatca aattttaaga taaactcttt tttattataa agataaataa catataactt
    19021 catattttag gtagattttt tcttatatgt ttaggagtgc atttaaattt ttccttaaaa
    19081 attctgtaaa ataattttgt attagtaaat ccatgagctt ctaatatact gcataaggta
    19141 tcatttgtat taattaagtc tttataaata tgagaaatac gcacatcatt taaatactct
    19201 aagtaagttt gtcccatata ctgtttaaat tttctacaaa aatattctgg ttgtaagtgt
    19261 gctaccttag cgattttgtt aatagaaata ggcattttgt aattaatctt tgtatattct
    19321 aaaactggtt caaattttga caaatcaagt actgtttttt tcatattttt attacctaca
    19381 gatattttaa agttagtata taattcaaat aaaagttcaa ataataagct tgtaaagagc
    19441 agattagctg caggaggaga actaagttta atttctctca tgttttctaa tataagttta
    19501 actttacttg aatttttttt atagttattg tcattggcat taaccttaaa gtcaaaatag
    19561 caatttttaa aatcagggat atatttgttt agaaaagaca atggtacttg caataatata
    19621 gaggtatttt gataaataca tctagttgag tgaataattc cagaattaat gaacatacac
    19681 tcattagctt ttaaattata agtgtaaccc tccatgttaa tttgtaaatc accagaagtt
    19741 atataaatta tttctaatga gttatgccaa tgatttgcta cataactacc actatcatca
    19801 aatagtttga aattaacatc taaattatca ataggttcaa ttatttcatg tgaaaaatta
    19861 tttctgttca taataatatc ctccttataa ttttaaatta taaatagtaa atttataatg
    19921 ttcaatatta aaaagataat atagattaat aaattagata ataaacatac ttaagaaaac
    19981 ggataaaatc aaatataata actgtatatt aaaataactt aaaaaaatta ttttattata
    20041 agtgcaaaat atacctattt aaataagata agaggggagg attttatgtg tatataaaag
    20101 gtgtaaattt aggaggttgg ttagttttag aaaaatggat gacctcaagt ttatttgaag
    20161 gcacagaagc tgaagatgaa tactatttac caagacagct ttcaagagag gcatatgaat
    20221 ctagaattaa gactcatcgt agtgaataca tcacagagag agattttgca atgattaaat
    20281 ctatgggatt taattcagtt cgtataccag taccatactt tatttttgga gattgtgaac
    20341 cattcatagg atgtgttaaa gaacttgata aagcatttgc ttgggcagat aagtatgggc
    20401 ttagtatatt gatagactta catacagttc caggaagcca aaatggattt gataatggag
    20461 gaatatcagg tatatgtagc tggtcacaaa atccagaata tgtagcgttt acattaaatg
    20521 tattagaaag acttgcaaaa cgttatggga tgaggcatga attgtatgga atccaaattt
    20581 taaatgagcc tataacagaa agaatgtgga acataatgaa tgttccaaat cggtttaagg
    20641 ctgtagataa ggagatggca agggggagta aacctaattc attagagttt ttacgtgatt
    20701 tttatataaa agcatataag gtaatgcgtc cttatatgag ggaggaaaac gttatagtat
    20761 ttcatgatgc atttgaatta aaagcatgga aagactttat gagagaggaa gaatttaaaa
    20821 atgttgttct agatacacac caatatttga tgttagctga agcagatgga tgtgaacaaa
    20881 gtattgatag ctatctcaaa tatattaggg aaaattatgc taaagatatt ttacaaatgc
    20941 aaaaatattt tcctgttata tgtggggagt ggagcttatt taattcatat gcttgtggta
    21001 tagatacaaa tggaggtcaa tcacctctaa atgggattga atcaaatata gataaattat
    21061 caaaagacga taaaagagaa ttatatagga agatagcaaa agcacagctt gatgcatgga
    21121 gaaatggaag tggtcactac tattggaatt ataagttatt actagataca gttaatgaag
    21181 aaggttggat aggatgggat agttgggacc tagtcaaatg tgtagctcaa gaatggtatc
    21241 ctatagaata ttaaactaaa attggaggaa aagatatgat taaaaaccct atacttccag
    21301 gttttaatcc agatccttgt atttgtagaa agggagatga ttattatctt gtagtttcat
    21361 cttttgaatg gtttccaggt atacctgttt atcactctaa agacttaaaa aattgggagt
    21421 tatatactca tattttgaca gatgaaacaa aaatagattt gaagaagctt ccttcatcta
    21481 aaggaatttg ggcaccctgt ttgacttatt gtgaagaaga agatttattt tatatagttt
    21541 atggaattat gaactctatg aatgcaagat attttgatgt tgataattat ttaattacat
    21601 caaaagatat aaagggagag tggagtgaac cagtatatct acactcatca ggttttgatg
    21661 cgtctatttt tcatgatgat gatggtaaaa agtggattgc atcattagac tgggagacaa
    21721 gagaaggata tgaaaaacct ggtgttatat gtttagtgga atactgtaca aaaaagaaag
    21781 aaatagtagg atatccaaag agaatatggt cagggggaac tgatagaggt tgtattgaag
    21841 caccacatat aacaaagcgt ggagattact attatataat gtgtgcagaa ggtggaactg
    21901 gatatggtca tagtgtaact atgggaaggg ctaaaaatat atggggacct tatgaaaaag
    21961 attcaatgaa tccaattgta acttcaattc caggcgattt ttatgaaaga catgaccctg
    22021 accatctaaa gcctaaatac tataatccag aatcaaaact tcaaaaatct ggtcatggaa
    22081 gctatataga aacaacatca ggtgaagtat atttagttca tctaacatct agaccatttg
    22141 taccagagtt aaggtgtact ttaggtagag aaacagctat tcaaaaaatg aaatggacaa
    22201 aggataattg gcttcgaatg gaagatgaaa gtaatttggc aaaagaatac gtatcagaaa
    22261 gtaaactaga agaacattta gtttcatcta taccaagttt tgatgacttt gattcaaatg
    22321 aattaggttt acagtactat gctcctagga tttcaccatt atcatttgca gatgttaagt
    22381 ctagaccagg atatgtaagg attagaggac aagaatcaag aacatcatta aataaagtga
    22441 gcatattggc aagaaaatta acttcagtat atgcaagaat aaccactaag atggagtttt
    22501 acccagaggt acatcagcat agtgcgggtt tgataatgta ttatgataat atgaattata
    22561 taaatttaag aaagtattat agtgaaacat tgggtcaaag tgcactatca ataatacatc
    22621 ttgaaaatgg agagaaaacg gaatttttaa atactaggat accaataaaa gatataccaa
    22681 tatatcttcg tctgtatgta caaggtcgta agtcatattt tgaatggagt tatgatgaaa
    22741 aaaactacca gagaatagga aaggtatttg atacaactaa gttttcagat gagtactgta
    22801 aatatggaga atttacaggt acgtttatag gattaacttg tgcagatcgt gtaaagcata
    22861 aacactatgc tgattttgat ttttttgaat atatagtaga tgaatcaaaa gatgtcgatt
    22921 aataaaatca ttactaaaat tttcagagca aaggtaaatc ttatgctctg aaataagaaa
    22981 cattagaagg caaatcttac aaaaaggagt atggtattat gaatacagaa gttaatgtta
    23041 aagcaactac gattagatat gaaaagattt cattttttga aagaatttct tatggttgtg
    23101 gggatttggg gtgcaatatc atatactcag ctatgtcagc atttttgctg ttttattata
    23161 ctaattatgc agatgttagt gcagcagctg taggtagtat tatgcttgta tctaggatac
    23221 tggatgggtt tagtgactta acaatgggaa ttatagtgga tagaactaaa tccaaatacg
    23281 gtaaggctag gccttggatt ttgagaatgg cgattccttt tgctatagca gcagtattat
    23341 tattttctgt tccatcaaat cttggtatca cttctaaatt aatttatatt tttattacat
    23401 ataacttagt ttctactgtt atttatacag caatcaatgt accctatgca actctaaatt
    23461 cacttataac tcaaaaccag tatgaaagag gagtattaag tatatttaga atgatattag
    23521 ccacttgtgg taccttacta ataaatggac ttacactacc tctagttgaa tactttggaa
    23581 ataatttatc agcatggact aaaacattct ttgtatttgg aatagcatct ataatggtat
    23641 tttttataac atttactgga accaaggaaa gagtaaaagc agttaagcaa gataggaatg
    23701 aagtaatacc atttaaaata ggtattaaat ctttatttag aaataagtac tggatacaaa
    23761 taacattatg tttagtttgc atatttattg tatttgcgat aaatggtggt tcatccgtat
    23821 attatgcaaa gtttattttg ggagatgaaa aattatttgg tcctataaat atggtttcaa
    23881 atatatccca aattatagct atgtttatgg ttgctccttt tataaaaaaa tttggaaaaa
    23941 gaaatgtatt aatagtaggt tcaataatat taatatcgtc aaatgttatg tttataatag
    24001 ttggacaaaa tcatatagga gtaatatttg caagtgctat aaagggaata ggaagtgctg
    24061 gaatagcatc aacaatgttt gctattgttt cagatactat tgaatatgga gaatggaaga
    24121 ctggatatag aactgagggg cttataaata gtgcatctag ttttgggttt aaagtgggaa
    24181 atggattagg ttcagcaata ttaggtgcag tattatcaat tggtggttat gtaggaacat
    24241 cagctactca aagtgattta gctatagtat caataaaagt atgttttata tacctaccta
    24301 tatttataac aatattacaa attatcataa tgtcttttta taagcttgat aaagaatata
    24361 gtactatact taatgaatta aatactaaat aatgaaaaag tttcttataa agtcttaagt
    24421 ttaattaagg tctattgtaa atgttcccta taaaaactat tatatattta tgattattga
    24481 agaattttta gagaagcgaa taataaaaat ataagaagac tttaaatgat gaaatggttg
    24541 gttttttaga agtgatttaa taagttattg ttatttaaag cattataagt ggacaattaa
    24601 tttttaattt atggagatga tatttaacaa ttagaagtaa tatataatat gcactaagaa
    24661 gcaatttttc aactatttat gaaggtggtg gtacaagaaa agtatcacct tttttttgtt
    24721 atacaaaaaa acagttgact ttttatatga accccttcta taatgttact gaaattaagt
    24781 aaaatgattg aacaaatgta aagataattg aaaagcttga ttttggagga aagcaaatga
    24841 aaaaaattgg agacttaaga ttaatgatga agtgttgttc actttattac gaagataatt
    24901 tgaatcaaca ggaaatagca aatcaattgg gaatatcaag accaacaata tcaagaatat
    24961 taaaagaagc ttttgaacaa ggaatagtaa aaatacagat agttgatgtt ttgaaaaatg
    25021 attaccaaaa aatagagaga agtttggaga gaaaatataa attaaaagaa gtaatagtag
    25081 tagatgataa acaggatgca ctaacacaaa aacaagaact agcaagagct gtatctgaat
    25141 atttaactag agtagtaaag gaaaatgata taataggtgt ttcaatagga acaacactaa
    25201 aagaaatacc aagatatgtg gaaaaaagta actgtaaaaa tgttacattt atacctctat
    25261 taggagggat tggagacaat gaaatagata ttcatgcaaa tcaaatagcc gtaagtttag
    25321 cgagggcttt tggaggtgat tttaaattat tacatgctcc agctgttatg tctgacttgt
    25381 ctacaaaaga gaaactttgc aaagacgaaa aaattaaaga agtactagat ttaatagata
    25441 aaacaactat agcaattgtg ggtataggaa atcctatgag tttaaactca acaataatgg
    25501 cttctggata catgaatgaa gcagatatag aagatttaaa aaaatacaac tcaataggtg
    25561 caatttgttt acaagccttt gataaagaag gaaaaacttc tatacttgaa tttaatcaaa
    25621 gagtattagg agtgaaactg gaagatttaa aaaagataaa aagaacaata ggtgtcgctt
    25681 caggagatga aaaaatagag gcaataaagg catcattaaa agcgaaattt ataaatagtt
    25741 tagcaattaa tcacagttta gcacttaaat tattagaaga ttgtgactaa tagaaagata
    25801 gataaaagtt aacaggaggg aatgtatatg tcaatatcaa aagaaacatt gcttgaaatg
    25861 tataagagaa tgaatcaggc tagaaaattt gaagagaaag ttagttggtt ttttgcaaga
    25921 ggtatggttc atggaacaac tcacttatct gtaggtcaag aagcgtcaag tgtggcggct
    25981 gtaatggctt tagaaaaagg agatttagta tcattaactc atagaggaca tagtcaattt
    26041 ataggaatgg gaatagacct aaataaaatg atggctgaac ttatgggtaa agaaactgga
    26101 ttttgtaagg gaaaaggagg ctcaatgcat atagcagata ttgagtcagg taacttagga
    26161 gcaaatggag ttgtaggtgg aggtcttaca atagctccag gagctgcact tacacaacag
    26221 tataaaaaaa caggcaaaat agttttatgt agttttggtg atggagcatc aaatgaaggt
    26281 acttttcatg aaggaataaa tttatcttca atatggaaat tgccaataat attttactgt
    26341 gagaataatt tatacggtat gtcaacaagc ataaaacgtc atatgaatat agaaagtatt
    26401 gccactagag cagcttctta tggaatagaa ggaatatcaa tagatggata taacccaata
    26461 gaagtatacg aaacagtaca aaaagctgct gagaagtgta gaagaggaga aggacctgta
    26521 ttaattgaaa gtagaactta tagatggtta ggtcattcaa aatcagatgc aaatgtatat
    26581 agaacaaaag aagaaataga atcatggaaa gcaaaagacc ctattgaatt tttaaaaaat
    26641 tatcttatag aaaataatct atctaatgaa gatgaattag ataagattca agaatttgca
    26701 aagcaatcaa tagaagatgc agtagagttt gctcaaaata gcccaaatcc aaaaatagag
    26761 tctttattag aggatgtata tgcagactaa gaatggtaaa ggaggaaaat aaaaatgagt
    26821 acaagggaat taacatatgc acaagcaata aaggaagcaa tgtcagaaga aatgagaaga
    26881 gatgaaaatg taatatttat gggagaagat ataggaatat atggaggagc atttggtgta
    26941 tcagttggta tgatagatga atttggacca gaaagagtaa gggatactcc tatatcagaa
    27001 gcagctatag caggagcggc agcaggtgca gcagcaactg gcttaagacc tattatggaa
    27061 gtaatgttta tggactttgt aacaatttct atggatgcaa ttgtaaatca agcggcaaaa
    27121 atgagatata tgtttggagg aaaagcacaa gttccaatgg ttgttagatg cccaggaggt
    27181 tctggaactg gttcagcaga gcaacattca caaagtttag aagcttggtt ttgtcatgta
    27241 ccaggagtaa aagtagttgc accttctaca ccagcagatg ctaagggatt gttgaaagca
    27301 gctataagag ataataatcc agtcatattt gttgagaata aattgttata cagaaaaaaa
    27361 ggttttgtgc cagaagatga ctatgtaatt gaaattggaa aggcagatat taaaagagag
    27421 ggtactgatg ttacagtaat tacttatgga agaatgttac aaagtgttga agaagcagca
    27481 gagactctat caaaagaaaa tataaatgtt gaaattatag atttaagaac tttatatcca
    27541 ttagataaag agactatagt aaagagtgta tgtaaaactg ggagagtttt aatttgccat
    27601 gaagcagcta agactggtgg gctaggtgga gaaatatcag cactgataac agaaagtgaa
    27661 tcatttgatt atttagatgc tccagtaaaa agaatttgtg gaaaagatgt gccaatacca
    27721 tataatccag agttagaaaa agcagttgtc ccaagagttg atgaaataga agaagcaata
    27781 aaatctttaa ttgttagata aggagggata gatatggtgg caaataaaat aaaagccaca
    27841 ccagcagcta gaagtcaggc aagaaaagac aatataaaat tagataggct gattggaagt
    27901 ggagaaaatg gaaggattca tttagtagat gtgttaaatt atttaaaaga taataaagca
    27961 aatactactc ctttagctag aagaatagca gaagatttaa atatagattt agaaactatt
    28021 gtaggtactg gatataatgg aaagataaga aagtgtgatg tggaaaaatt aactgcaaaa
    28081 gaaactattg tatcgactaa tacatctaaa tctagtgaga aaaaagagct gaaaattgaa
    28141 aatgaaaatt caagcatgtt taacacagta gaaggaatat ttgaaaagcc aaatccaatg
    28201 agagctactg tagcaaaacg aatgtcagaa agttatttta gtgctcctgt atttacattc
    28261 aatatagaag tagatgctac agaattgaag gtacttagag caaagttaat cgacacagta
    28321 aaagaatcaa ctggagtaaa attaactatg acagatttaa tagtaatggc agtatctaaa
    28381 atacttccaa atcatcaagc cttaaattca gcatggacag atgagggtat atttagatat
    28441 aaagatgtaa atatagctat tgcagttggt ttagatgaag gcttatatgt tccggtagta
    28501 aaaaatgcaa acaaaaagtc tttaaaagaa atagcaaaag aaagcaaaga acttgcggaa
    28561 aaagtaaaga cagggaaact tatgccagca gaccaagaag gtaatacatt tacaataagt
    28621 aatgttggaa tgtatggtat aactacattt acacctataa taaatatgcc ttcaagtgca
    28681 atcttagggg taggtgctac acaagataag tttgttccag taaatggaga ggctaaaata
    28741 aaacctataa tgaacttatc attaacatca gaccatagag taatagatgg aacagtagct
    28801 gcaaaattct taaaagattt aaaagaattg ttagaaaatc ctttatcaat gcttgtatag
    28861 gttttgatat tttgttactg atataaatag tttgaataga ttatattatt ataattgttt
    28921 cagtaaattt aaagctttta tatattaaga attattttta acagtaacta tatgtgaata
    28981 tgatttgctt aattaaaata taagaaaata aataggagga gagcctatgt cagtagaagt
    29041 aataatgcca aaagcaggag tagccatgga agaaggtact atagtatctt ggctaaagca
    29101 agaaggcgaa gaagtaaaga ttggagaacc tatactagaa ataacaacag ataaagttaa
    29161 tatggagata gagtcagaag gagaaggtac acttgctgta atcatacata aagaggaagg
    29221 tgaagtatta ccagtattta cagtaatagg tgtaatagca gaaaaagggg aaaatcaaga
    29281 ggaagtaaaa gctaaatatt tatctggaaa tgtttcaaaa gaagatatag ttgaagagaa
    29341 tcaaaatata gaatttaaag aagaaaaaat aaacaaaaaa gagtgtaatc atgattatga
    29401 tgtagtggta ataggaggag gacctggggg atatttatca gcattaaaag cagctctttt
    29461 aggtggaaga gttgctttag ttgaagaaaa tatactagga ggtacttgct taaatagagg
    29521 ttgtatccct acaaagactt atataaaaac agcagaaata ttagaagaaa ttgaccaatt
    29581 atcaaagaga ggtgtaaaag taactgtaga taaagaacag gacattaaga aggccataaa
    29641 atataaaaat agagtagtta aaaaacttac agctggtgtt ggcggattat taaaaagtag
    29701 agatgtagat gtgtttaatc ttaaagctag tgttaaagaa gagcataaag taattttatc
    29761 agatggaaaa gttttagata cagaaaatat aataattgct acaggttcca aagtaaggat
    29821 attacctata aaaggaatag aatcaaattt aataataact agtacagaag cattagattt
    29881 ggaaacagtg ccagaagaat tagtaataat tggtggtgga gtgattggtt gtgaatttgc
    29941 agagatattc aattcaagag gttctaaagt aactatagta gaaatggaag atagagtaat
    30001 cccaagaatg gataaagagt taagcgaatc tttaaaatat tctcttagta aaaaaggtat
    30061 aaatgtatta actaaaaaga aagtttctga atttaaagaa gaaggaaata acatattagt
    30121 gtgcattgaa ggtgaagagc caataaaagc agatttatgt ctatatgcta taggaagaga
    30181 agctaatctt tctggcatag aggacttaga tattaaaata gataaaggtt caatagttgt
    30241 aaatagtaaa atggaaacaa gtataccaag catatatgca gtaggtgatg ttactggagg
    30301 tgtaatgtta gctcatgctg cttttaaaat gggagaggta gcagcttcaa atgctctagg
    30361 tgtgaataaa gaagttgact taggtgcatt acctagttgt gtttatacaa ttccagaagt
    30421 agcatcagtt ggtattacag aagaggatgc tagaaaaaaa tataatgtaa aagttggtaa
    30481 gtttaatttt gctggaaatg gaagagcttt agcttctggt caagaacaag gatatgtgaa
    30541 ggtagtagca gatgctaaat atggagaaat tttaggtata catatgtttg gatgtggtgt
    30601 agcagaactt ataaatcatg cagcatcatt taaagcatta gagataccta ctgatgaagc
    30661 aagtgagttg atatttggtc atccatgtac ttcagaagct ttaatggaag ctttggcaga
    30721 tgtaaatgga gaatgtttac acttacctaa aaaataaatc taaacataaa ttattaattt
    30781 aagtctatta ttaatattaa tttatatcat aaaattttaa aacacttact tagccatacc
    30841 ccttattatg caaataattt atttgcataa taaggggtat tttttatgga aaaatataga
    30901 atatagaatc cttgtagttt ttgttgtaca aaaaagatag taatataaaa taacattatc
    30961 attttagaat gatttcaatt gtaattagga aaagtgattt ttttgttttt aaaaaaagta
    31021 ttatttgtaa aatataaaaa aaaatttctt aatatttaaa taaaagaata gcttaattta
    31081 aatgttatta ctttataaaa aaataataag tacaaaaaaa acaatatatg gcatataagt
    31141 tgctatataa ataataagga gggaacaatt ataaatgagg caaagaataa aaaacttaat
    31201 tgaaaatgaa gataagaaaa atcctttaac agatgaagcg atttcaagta ggttaaatat
    31261 tcgaagagaa gatgttacct ttttgagaaa tgagttaaaa atagaagatt caagacaaag
    31321 aagaaaagtt gttttaatta aagctataaa agatatttta aaagaagaaa aaactataaa
    31381 taaaaatgat ataacaagaa gattaaacag cattgggttt acagtatcaa gatttacagt
    31441 catacaatac ttaaaagaga ttcaagattc tggtgaccta aaacttgata aagagagtga
    31501 tgataaaaaa gaagaaataa tcaataatag agaaaataaa gatatagcat ttgataaact
    31561 cataggtagt agtggtagct taagtacaat tataaaacta gccaaagcag caattcttta
    31621 tccacctcat ggtcttcata ctattattat ggggccaact ggtgttggaa agagtgagtt
    31681 agcagagtgt atgtataaat ttgcagtaga atcatccaga ttccctaaga atgcaccatt
    31741 tatagttttt aatgcagcag attattcaga gaatccacag cttttgctat cacagttatt
    31801 tggacacgtt aaaggtgctt ttacaggagc tgatgagaac aaagaaggat tagtatcaaa
    31861 ggcagatgga ggaatattgt ttatagatga aatacataga ttacctcatg aaggtcaaga
    31921 aatactattt caactgattg ataaaggtat ggtcagaaag ttaggagaaa caagactaac
    31981 acataaaata gatgtaatga ttatttcagc aacttctgag ccaatagatt ctcatttact
    32041 taatacattt aaaagacgta tacctgtaac aattgaaata ccagaattag ttgcaagacc
    32101 attaaatgag aggtttgaca ttataaataa tttcttctta atagaagctc aaagaatggg
    32161 agcaaacata catataaagt cagatgtatt aaaagctctt atgctttacg attgtatagg
    32221 taatgtaggt caacttagaa gtgatattca agtagcttgt gctaggggac tattaaacca
    32281 acttacaaac aaactaaaag aagtaaatat aagcatatca gatgtacctg gatatgtaaa
    32341 acaaggtctt ataaaaatca gaaattgtag aggaaaaata gaaaattatg tagatgggga
    32401 tttaattata gactcaaatc ttcaaggtga gatagaaaag aaaaatgaag atatatatac
    32461 ttttccagat gaaatataca aacttactga aagaagacat attgatttat tggaacaagg
    32521 tttagatttt gatgttataa atagaatcat tggaggagaa ttagaaagtt gcattcaaaa
    32581 atacataaaa caagttaaaa aagtttctat tagtaacaat cttccagata taagtccaat
    32641 tgttggaaaa tatatcgtag agctaactta tgaagtaatc aaaatagcat cacaatacct
    32701 aagttcacta gaaccgaatc tacatttatg tttagcagtt cacctaaaag cagcttttga
    32761 gagattaaaa gaaggaaaag taatatcaaa tgctcatttg aaagaaataa taggaaatta
    32821 tccactagaa tattctgtag cagaaaaggt agctcaatat ttaaaggaaa gatataacat
    32881 aaacttacca aaagaagaaa taggtataat agcaatatat tttaaaatga gtagtagaag
    32941 agatatgtct gaacaaaaaa agataggtgt acttcttatg gctcacggaa gagtagcatc
    33001 ttctgtatgt gaggttacaa ataaactatt aggaattaat cacgcaaggt atttagatat
    33061 gccatttgag aagaaaccgg aagaaatgat tgaagaagct actacaataa taaaagatat
    33121 tgatgaaggt agaggtgtga ttatattagt agatatgggt tcactttcat attttggaga
    33181 tattatttct ggaaagactg gaattaatat aaggacaata tctagatttg atacattgct
    33241 ttctatagag gttatacgaa gagcagtttt accagattta acaatagacg agatagttga
    33301 tgaagttaaa ggtatagagc cacaagtaaa agaaaaggtt agtaaatgta ataatacaaa
    33361 agtagatttt agaaaaccta taattttaac attatgtatt actggattag ggggagcttg
    33421 taagataaaa aaaatcctag aggaaaatat taaaagtata actgaaaagg ttgatataga
    33481 acctataggt atgattgaat atccaagtct tgatttagag ataaaacaaa taagcaatga
    33541 taaaaatgta atcgccatag ttggaacaat tgaccctcag atagcaaata tccctttttt
    33601 tagtctagta gacataaatg ataaagcaaa gttaaatcaa ataaataacc taattgatac
    33661 aaatcaaatt ggtgaaacaa ataaaaaata tgaattatca gatttaatac ataaagaaga
    33721 tatatttgtg gatttacagg ttaaatcaaa agaagaagtt ctaaaatata tgagtaaaaa
    33781 acttagagag aaaggttatg tgacaaaaaa tttctataaa aaagtagtgg aaagagataa
    33841 cttatttcca actgaattat cagatgaaat agcaatacct cacacagatg gaatagatgt
    33901 aattaggcca gctataagta tagccatctt aaagaagcct attgtgtgga gtaaaagtaa
    33961 agtaaaaata atacttttat tagctgtaga ccaaaagtgt tttaatccat taagcactct
    34021 actctcattt atagaaacag atgaatttaa gaagataaaa aacattaaag atagagattt
    34081 tattagggag atgatattag atggagtcaa tgaaaataat tagtagcaat ttaattttta
    34141 aaaatattga agtgtctaac aatgaagatg cattaaagtt tttgggacaa cgtttgtttg
    34201 acgaacagta tgtaaaagaa agctacatac aagctgttgt agcaagagaa aaaaagtatg
    34261 ctacagggtt accaacagaa atatacggag tggcaatacc acatacagat atagtacatg
    34321 taaatgagcc aggaatagca attggaatac tgaataaacc tgtaaaattt ataatgatgg
    34381 gtacagacga tactgaaatt gatgtaaaag ttgtatttat gttagcagtg aaagagccac
    34441 aagaacaatt gcaactatta gaaaaactta tgactatatt tcaagataag aatatactaa
    34501 ataatattgt tgacttaagt gaagaaagtg tgagtgattt attaaactct aaattgaaaa
    34561 attaaacatg taaagtatat taaaaatgta aaattaaaca tgcaaagtat gttaaaaaat
    34621 ataaaaagat tattaaagat ttctaactat agatatcttt aaaataaaat aatttcaatg
    34681 ggggtgacta atgtggctaa aaagatatta gttgcatgtg gaacaggagt atgtacttca
    34741 acaattgcta taaacaaatt aaagaaagca cttcaagaca ttggtaaatt ggatatggtt
    34801 aatattacac aatgtaaagt agtagaagtt gcatcaaaag cacctgatta tgatttaata
    34861 atttgtacta cacaagtatc tagtagtata aaaacaccag tgataaatgg attacctttt
    34921 ttaactggtg tcggaatgga taaattaatc aatgatgtat tagaagagtt agaattataa
    34981 ttaaaaaaat taaggaacat ggtctctaaa atcataagtt ttagagactt aaaattaaat
    35041 aatggagggt ctttatatgg agatattaaa tttcattgtt ggtctaggtg cacaagtaat
    35101 gatgccaata ataatatgta tttttggttt gatattaggt acaaaactag gaaaatcttt
    35161 aagagctggt ttaactgtgg gagttgggtt tataggtctt aacactatta tagctttgct
    35221 aacagataat ttaggtccag ctacacaaca aatggttaag aatttagggc taagtttatc
    35281 aataatagat gtaggatggc cagcagcatc agctatagct tttgcatcaa cagtaggggc
    35341 tttaattatt ccaataggtc ttgtggtaaa tatagttatg ctaattacca atactactca
    35401 aactgttgat gtagacatat gggattactg gcattttgct tttacaggag ctttagtggc
    35461 aggggctact caaagtgtaa tgtggggagt atttgcagca gtagcaaata tggtaattgt
    35521 attggtaatg gcagatttaa ctgctccagg tatagaggaa tatttaggta tgccaggtat
    35581 atctcttcct catggattta cacaggcatt tgttccaatt gctatagtag taaataaatt
    35641 attagactta ataccaggca taaataagat tgagataaat gctgatacat tgcagaaaaa
    35701 atttggatta tttggagaac ctttaataat ggggtctgta ataggtgtaa taattggtat
    35761 tgctgcgaaa tatgatataa aaggaatatt acaattaggt gtaactatgg gagctgtatt
    35821 aatattaatt ccaaagatgg cagcattatt aatggaagga ttattaccag tatcagaagc
    35881 tgcacaggaa tttattgaga agagatttaa aaacagaggc aagatttata taggcttaga
    35941 ttcagcagta ggaataggtc atcctgttac attatcagtt gcactagtgt tggttccttt
    36001 gactatactt atagcagcta tattgccagg aaataaagtt ttaccatttg cagatttagc
    36061 agtaattcca tttgcacttg tattaatcgt accaattaca aagggaaatg tgtttagaac
    36121 attaataata ggaataatta taattacttc tggattatta atcgcaacta acttagctcc
    36181 attatttacc caaatggctt taaatgctag ttttaaaatg cctgaaggag caactatgat
    36241 ttcaagtatt tgtgatggtg cgaatccatt aagttgggta tttgtaaaag ttatgaacta
    36301 taaagttatt ggaggagtag tttttggagt tatagcttta ggaatggcta tttacaatag
    36361 gaatagaata ataaatgaaa ataaaaagct atctatagaa gaataaggtt aaaacaattt
    36421 tattaaggag tgtataggtt atgaaaaaat ataaatatga ttctaaaaga tacttactca
    36481 gtgttacaat accaggattt tttatgattt taatattgat ttatgcactg tttaataact
    36541 atacaaattt taatttgaat atatatagtt tgttaataat tgtctgtact tatggtatat
    36601 tcaatacttt tatatcatca agtaatccta aaaaaattat tattgatagc aaatgtatac
    36661 attttacctc atttatgact acacataagt atgaaattaa agatatagaa aagataagaa
    36721 taagagaatt ttataataaa aaaatttatc taaaaattaa tgatggaaaa cttttcaaag
    36781 gtaggtattg gataagaaca aatatgttta ataatagtat agcattatat agctatttca
    36841 ttgagttgga agagtgctta tatccagatt cactaaaatt tagaaataaa aaatttgaga
    36901 ataagaacat ataaaaataa aaaattgaat tgaagcctag cttaatcttt tttgataaga
    36961 tataagtttg aactttatac taaattttta gaattttcaa tcaagacaat aacataatag
    37021 ctaagcaatt agaacacaac taagatgtat aaggcatgta tatgatgaat gggaggattt
    37081 aattaatgtt attagagaaa ttaagaaaag aagtattaca agcatcttta gaccttttaa
    37141 actataattt agtgacatta acaggaggaa atgtaagtgg gagagatgaa caaactggct
    37201 atatagcaat caccccaagt ggaatggatt atagaaatct tacaccaagt gatattgtta
    37261 tagtagatgt agatggaaat attatagatg gaaaatggaa agcatcagtg gatttatcag
    37321 accacttgta tatatataag catagagaag acataaatag tataattcat acacattcta
    37381 catactcaag ttgttttgct attttaaatg aacctataga atgtgcaagt acaactcttg
    37441 caaatgaagt agggggttca gtaccagttg caaaattttc gccaccaaca tctaaaaaga
    37501 tgggaaaatg tgttattgaa gcaattggtg acaaaagagc atgtctttta gcaaatcatg
    37561 gcgtaattgc agttggtcca tctgtaggac atgctcttac agccgctgta atgttagaag
    37621 attcagcaaa agtatattac ttagcaaaat caattggcac accagtgcta ctgcctgatg
    37681 aagaaattca aagagcgaga gatgtatttt ttaatgtcta tggtcaggat aaataaatta
    37741 gataaaattt taaaagtaat atgaaaatat agatattcaa tgatattaag gagatttata
    37801 atgatataat aagtttaata gttaagaagt aaaatatgaa atgtaatagt atataaattc
    37861 atttaagact aaaaagaata agataaaata aaaaggagtc tgtatttcta tgtattactt
    37921 attaaacaaa agcacaaatc cttactttaa tttggcatta gaagaatatc tatttttaaa
    37981 tgataaatat aatgatgata ttataatcat atggagaaat gaagaatcca tttttatagg
    38041 taaaaatcaa aatccatatc aggaagtata tcatgatgtt attgaaaagg gagaaattcc
    38101 tattttaaga agaatatctg gaggaggaac tgtttatcat gacttaggta atatcaatat
    38161 gtcattcata caaaaagata ggcaacttca tgagatagat tttttggagc atactaagtt
    38221 tatgcaaaag atgctttcaa ccttaggact agatgtatct ataactgaaa gaaaagattt
    38281 atttttaaat gggaaaaaaa tttcaggctc tgcacagtct ataaaaagaa aaaattctct
    38341 ttatcatgga actcttcttt acgattcaga tttaaataaa ttaactaaat atttaaattc
    38401 aaataaagca actgaatcaa atgcaactaa atctgtttca agtaaagtta caaatattaa
    38461 gtttttactc gaaaaagata taaattactt tttagattat tgtgttgaat atttaaaatc
    38521 taatataaag aatataaaag aacttgaatt ggatgaagaa gatattaaaa gtgtatacaa
    38581 tataggagaa gataaatata aaaagataga gtggacatat ggtaaaacgc cgaaatttca
    38641 agtagtgcta ccatttaata atgaaagtaa aataaatata catgttaaca gatggaaagt
    38701 atcaaaattt tttatttctt atgatgataa tgtaattaat atggacaagt tgttagattt
    38761 gaatttcttt aaagaagaaa taaaagagag tattttagaa aattatccac attacataga
    38821 tttaatcaat ttaatatttt aatataaaac ctctaatttg ataaaattgt cttatatcaa
    38881 actagaggtt tttaatattc ttgcatatta acaaccatta caaaattgta aaattaaaaa
    38941 caacactatt gaaaaatcaa tatatattgt aataaatata tgtgtttttc attagcaaat
    39001 agacattaac tatttataca actgaaaatt taatatataa ttaatcagat ggttaaaata
    39061 aaaatattaa aagttttaat aaatgatggt taaaaataaa atacgaggtg ataccatgta
    39121 tagtgttata attgttgcag cgggaagcgg aaggagaatg aacttagata taaacaaaca
    39181 gttcataaaa ttaagagaaa aagaaattat tgctcataca attcaagttt tttatgaaaa
    39241 tataaatata gatgaaatag tggtatgtat aaaaaaagaa gaagaagatt tttttaaaga
    39301 aaatataata aataagtata actttaaaaa tataaaaata gcatatggcg gaaaagaaag
    39361 acaggactca atatataatg gtttaaaaaa attagataaa aattgtgata tagtacttat
    39421 ccatgatgga gcaagaccat ttgtcgacca tagaataatc aatgaatcta taaaggtagc
    39481 taaagaaaaa aaggctgtgg ttgtaggtgt tccagtaagt gatactataa agatagtgtc
    39541 agatggaaca gtacaagaaa ctccagaaag aaatttgctg tgggcagcac aaacccctca
    39601 aacctttgaa tataatctca taatagatgc ttatgaacaa gcatataaga acaattatta
    39661 tggtactgat gattcaatgt tggtagaaaa tataggtcaa agtgtcacta tggtaatggg
    39721 ttcttatgaa aacataaaaa taactagtcc agaagattta aatatagcag aacaaatatt
    39781 aaatatggaa aaaagagatg aagttaacag tagaaggaga attatatgag aataggatta
    39841 ggatatgatg tacacaaatt aacagaggat agaaaactta taatcggtgg tgtagagata
    39901 ccacacgata aaggtttgtt gggtcattct gatgctgatg tattgataca tgcaataatg
    39961 gattctattt taggtgctct agcattagga gatataggga agcatttccc agatacagat
    40021 gaagaatata agggagcaga tagtatgaaa ttattagaac atgtgtataa tctaataaca
    40081 agtaaaggat ataaaattgg taatatagac agtactataa ttgctcaaag ccctaagatg
    40141 gctccatata ttgagagtat gagaagtaat atatcaaaag ttttaaacac agacattgat
    40201 aatataaaca taaaggcaac aacagaagaa ggtcttggtt ttacaggagc aaaacaggga
    40261 atagcttcac aaagtatttg tttattattg ttgacaagtc agaataatta atatattatt
    40321 agtaataagt ttaatggata tcaagtaata ggcctatata gaatatctat aatgtatgaa
    40381 tttaagtgaa attaatataa atataaaatt agctatgata aagaaatagt aaaatgatga
    40441 aatcttcaca gagagaaaac ggtggtgaga gttttcaaga ggaagtcttt gaacctgtct
    40501 tttagcttaa aggtataaaa atctttacgg taggaaccgt tatattccca aaagagagct
    40561 attcatatag ctaactagag tggtaccgcg gaaattaaac ctttcgtctc tatacttgta
    40621 tagagatgag aggtttttta tattttcagg aagttaatat tcaggaggaa tctattaatg
    40681 aaaatgtcaa aaatgtttat gccgacatta aaagaaatcc cagcagatgc tgagattaca
    40741 agccatcaat taatggttag gtcaggcatg ataaaaaaaa tgacttcagg tgtttataac
    40801 caattaccaa tggggttaag agtgttcaaa aaaattgaac aaataattag agaagaatta
    40861 aataaaaaag attgtcaaga aatactttgt gcagccttac ttccttctga attatggaaa
    40921 gagtctggaa ggtggactgc tatgggtgaa gaaatgttta gactaaaaga cagaacagaa
    40981 agagagtact gtcttgggcc aacacatgaa gaagcattca ctgatataat aagacaagaa
    41041 attacttcat ataaacaatt gccactgaac ttgtaccaaa tacaagtaaa atatagagac
    41101 gagagaagac caagatttgg tgttatgaga actaagactt ttacaatgaa ggatgcctat
    41161 agttttgatg tagatgataa aggtttagat aagtcatatc aagatatgtt tgatgcatat
    41221 gtaagtatat ttaatagatg tggattggag aatagccctg tacaggcaga ttcaggagct
    41281 ataggaggtt ctacatcagc cgaatttatg gttaagtcag aagttggtga agatgaagtt
    41341 gtattttgta gtggatgtga ttatgcagcc aatgtagaaa gagcagaatc ttgtaatcta
    41401 gcatcacaaa aagaagaaat gaaagaatta gaagaggttc atacaccagg agcagctaca
    41461 ataaaagagt tagaagaatt tttaaaaact tctccagata agtttgctaa aactttagta
    41521 tatgaggcag atggaaaaac agttgtagtc gttgtaagag gagatagaga agttaatgaa
    41581 atcaaagtat ctaatgccat aggttctgtt atagaatttg ctcttgctac agatgatgta
    41641 gtgagaaaag taactaatgc agaagtagga tttgcaggtc caataggtat aaatgctgac
    41701 tatgtattta tagataaaga aattgttgaa caaagaaaca tagttgttgg ggctaataaa
    41761 actgagtacc atataaaaaa tgctaactat ggaagagatt ttgaaggaat agttggagac
    41821 tttagaaatg ttcaagaagg cgacaagtgt atagtatgtg gtaagccact tgaaatagct
    41881 agaggtgttg aagtaggaca tatatttaaa ctaggaacta agtactcaga atctatgaat
    41941 gcaaacttca tagacaaaga tggaaaatca aagcctatag taatgggatg ttatggaata
    42001 ggtgttgaaa gaacagcagc agctataatt gaacaacata atgatgagaa aggtataata
    42061 tggcctttgt cagtagctcc ataccatgtt gtgatagttc cagcaaatat gaaaaatgaa
    42121 gaacaaatat caatagctga aaatatatat aatgatttgc aagctatggg tgtagaagtt
    42181 ttattagatg acagagatga aagaataggt gtcaaattca atgattcaga attaatagga
    42241 atacctatga ggattacagt aggtaaaaat attaatgaag gtaaagtaga atttaaactt
    42301 agacataaag aagataaaga gataattgac atagaagaaa taaatgaaaa agtaaaggca
    42361 gagtttatca ggaataatgt taggttagga taataactta aataagatat tgtatagata
    42421 atcaaagttg tactttatag acaccatttt atctatatat aaggatataa aatggtgttt
    42481 ttttataaaa caaataaatg aggaggtaat taaaatggca aaaaatgaaa aacaatttgt
    42541 tgaagaaata acaaagatgg aagatgattt tccacaatgg tacacagatg taataactaa
    42601 gactgactta gttgattatg ccccagtaaa aggttttatg gttgtaaagc catatggata
    42661 tgcattatgg gaaaagatgc aagaatttat ggataaaaaa tttaaagaaa ctggacataa
    42721 aaactgttat tttccattat taataccaga gagtttatta aacaaagaag cagaacacgt
    42781 tgaaggtttt gctccagaag ttgcatgggt aacacatgga ggaaataaaa aattagaaga
    42841 aagattatgt gtaagaccta cttcagagac tataatatgt acaatgtatg cgaaatggtt
    42901 aaaatcatat agagaattac catatcttta taatcaatgg tgctcagttg ttagatggga
    42961 aaaatcaact agacctttct taagaacatc tgagttttta tggcaagaag gtcatacgtt
    43021 acatgaaaca gcagaagaag cacaagaaga aacaatacaa caattagaag tatataaagc
    43081 gctatgtgaa gaattacttg caatgccagt tgtagctggt caaaaaagtg aaagtgagaa
    43141 atttgcaggt ggtgaaagaa catatactat agaagctatg atgcatgatg gtaaagcatt
    43201 acaatcaggt acaagtcact tcctaggtca acactttact aaggcttttg atattacttt
    43261 tgcagatagg gaaggaaatt tagctaatcc ataccataca tcttggggag catctacaag
    43321 acttataggt ggacttataa tgactcatag tgataacaga ggtttagtat taccaccaag
    43381 agtagctcca attcaagttg ttatagttcc tatagctgct aaaaaaggaa atgtaatgga
    43441 gactgttgat aaaatatatg ctgatttaaa agctaaggga gtagctgtag aagttgatga
    43501 tagagataac tatacaactg gatggaaatt taatgagtgg gaaatgaaag gtgttccagt
    43561 aagagttgaa attggaccaa aagatataga aaataatcaa gctatggtat ttagaagaga
    43621 tactttagaa aaggattcta tgcctttaga aggattagct gatgctatat gtgatttatt
    43681 tgatgttata cacaatgata tgtttgaaaa agcaagaaaa catagagaag acaatacatc
    43741 tatagttgaa aatatggatg agtttagaaa agctttagaa gaaaagccag gattcataaa
    43801 gactatgtgg tgtggagatg cagaatgtga agctaagatt aaggaagaaa caggagcaac
    43861 tataagatgc ttaccttttg aacaagaaaa cttaggtcac aagtgtgtgt actgtggaaa
    43921 agaagcagat agtatggtag taatggcaaa agcttactaa aatacattta aataaaaact
    43981 ggggtagaaa gtatttaata ctttatccca gttttttgtt ttttatgagt aataaaatgt
    44041 atttgtaact atgtcataag ataagtcata gatacttata ttaaaaagaa tataatagag
    44101 aactattttt aataattaca tattaatgaa agcaaaatat tttgttatat agattttcaa
    44161 taaagcaaac aataaatata aatgaatatt aataaggagt gcttttaatt tttagaaaat
    44221 aaatgactta aaagaaagat ttatgaaatt aaaactagga ttttctgata taataaacat
    44281 gtgtattttt aaataaaaag gatattatta ttagatttga ttaaaaataa acttgcaaaa
    44341 caatgtacta gtttaatatt atagttatat tgaataaaat atcaggagga agtaaaaatg
    44401 aatgtaagag taaggtttgc accaagtcca acaggatttg tacatatagg tagtttgaga
    44461 actgctttat ataactattt atttgcaaaa aaaatgggcg gagaatatat attaagagtg
    44521 gaagatacag accaaagtag attagtagaa ggtgctatag agaatatgct taatgctatg
    44581 aaatgggctg gggtcaacca tagtgaaggt gttatattag atgatagtgg aaaagtagta
    44641 caaaaaggag aatatggtcc atatattcaa tctcaaagat tagatatata ccaagaatat
    44701 ataaagcaac ttttagatag tggaaaagct tattactgtt tctgtacaaa agaaagactg
    44761 gatgaagtaa gagatgctca aagagcagca ggagaaacag ctaaatatga tggtcattgt
    44821 aagaatcttt ctaaagaaga agtagaagcg aatataaaag caggaatacc atatgttata
    44881 agattaagat tgccagagaa tcatacaatt aagtttacag acttagttag aggagatatg
    44941 gaatttaata ctaatgactt agatgaccaa gtattaatga aaacagatgg atttccaact
    45001 tatcattttg cagtagttgt tgatgactat ttgatgaaaa ttacacatgt tataagagga
    45061 gaagaatggg tatcatctac tccaaaacat gtttatttat atgaagcttt tggatgggaa
    45121 gcacctgtat ttgtgcattt accaaatata cttaataaag aaaagaaaaa attaagtaaa
    45181 agacaaggtg atgtagctgt tgaagacttt aagaaaaaag gatatttacc agagggatta
    45241 gtaaactatg tagcattagt tggttggtct cctgaagata atcaagaatt gtttacaatg
    45301 gaggaattag aaaaagcctt ctctgtagaa agagtatcta aaagtggtgg agtatttgat
    45361 actgaaaaat taaattgggt taatcagcac tatataaaag atggtgatga tgcttatcta
    45421 acagacttag caataccatt tttaatagaa gatggattta taacagaaga agaagctact
    45481 aataaatatg atttcttaaa gagtatgata tcagtactta aagagaaatt acaatatgta
    45541 aaagaagtta ctgaacatgc aagtatattt tttggagata aaatagaggt agaaactgaa
    45601 gagggaagag agttcttaag attagaacat atacctactt taatagatgc tttgagagaa
    45661 aaaatagaaa aaacagaggt attaaatgct gagtttgttc aagctatgct taaagaaata
    45721 caaaaagagt acaaaataaa agggaaaaat ttatttatgg gctcaagaat aattttgaca
    45781 gggcaaatgc atggtccaga tttaccaaaa gtaatggaag ttttaggaaa agaaacttgc
    45841 ttaaatagaa tagcttatgt aaaaaataat atattatagt cataaaagtc cgctaattta
    45901 gaagcggact ttttttgtgt tagtatttgg tgaaaatatc tgcttaagaa aatgaaggta
    45961 tttaaggtaa taaatttaat atataaaaag caaagcaaaa atataaataa aatatgaata
    46021 ataaactaat aaaatattaa gcatagaaga agggaatcta ttattttagt ttgatttaat
    46081 tattaagggg gtaattacta tgtatagtaa tattgatgat gttaaaaagg aattaaagga
    46141 gctatgctta gaatatgtta caattttaga aaaattaaaa gatgaaaaaa tgattacaga
    46201 agaaactttt gaaaagtgct cttctcaaaa aaaaatattt ttagaagaac aatagaggat
    46261 ggtaatagta ttattattat gtagtttgaa agatagtacg aaaaatgata gtaatactat
    46321 tatttattgc aattttcaac tataatataa tatataatgt taagaagata atttataaaa
    46381 atacagtgag gtgatgagtg tgaaagttta taatacattg actagaacaa aagaagaatt
    46441 tgttccatta gaagaaggaa aggtaaaaat gtatgtgtgt ggtccaacag tttataacta
    46501 tatacacata ggtaatgcaa gaccatttat aatatttgat actctaagaa ggtatttaga
    46561 gtatagagga tatgatgtaa cttatgtaca aaactttaca gatgttgatg ataagattat
    46621 aaatagaagt catgaggagg gaatatctcc tgaagaagtt gcggctaagt atataaaaga
    46681 atattttgta gactgtgatg ggctaggaat aaaaagagct actgttcatc ctcaggttac
    46741 agataatata caacagatta tagagtttat aaaagagttg gaagataagg gatatgcata
    46801 tgcagtaaat ggtgatgtat attttgatac aaataagttt gaaggttatg gaaaactttc
    46861 aggtcaaaaa caagaagatt tagaggcagg ggctagaatt gaggttaatg accaaaaaag
    46921 acatcctatg gactttgttc tttggaaggc aaaaaaagaa ggtgagccag gatgggatag
    46981 cccttgggga gaaggtagac caggatggca tatagaatgt tctgttatgt ctaagagata
    47041 tttaggtgaa acaattgata tacatgcagg tgggcaagat ttaacattcc cacatcatga
    47101 aaatgaaata gctcaaagtg aagctagaag tggaaaaaca ttctcaaaat actggatgca
    47161 taatggatat ataaatataa atgatgaaaa gatgagtaaa tcaaaaggta atttctttac
    47221 agtaagagat atatcaaaat tatatgattt agagatagta agatttttca tgttatcagc
    47281 acattataga aatccagtaa actttagtga tgagatgtta aatcaagcta aagcaggtct
    47341 tgaaagacta tataatacta aagaaaaatt agaatttacg ctaagtaatt tagtagaatc
    47401 tcctttaaca gaaaaagaag tagagcttgt taaagaatta gatgacttta gacaaaaatt
    47461 catagatgct atggatgatg atgttaatac agcagacgca gtaagtgtaa tatttgaatt
    47521 agctaagtta ataaattcta atgtagatga aaattcttct ttggagtttg ctaaaaaatg
    47581 cttagatgaa tttaatgaac ttacaggagt attaaatatt gtaaataaga aaaaggatac
    47641 tgtattagat aaagacattg aagaattgat acaaaaaaga actgatgcaa agaaaaataa
    47701 agagtttcaa ttagctgatg atattagaca acaattacta gatatgggaa ttgtattaga
    47761 agacacaaga caaggtgtta agtggaaaag gatataaaaa tggaaaaaac tgaattagtt
    47821 actatgtcac cattagtttt agcatactta ggtgatacag tgtatgagac atatataaga
    47881 gaatatttaa ttagacagaa tacacaaaga aaagttaatg atttgcataa actagctata
    47941 aagtatgtaa aagctaaagc tcaggcaact ataattcatg aagttgaaat tgagttaact
    48001 gaagaagaga gtaaaattta taaaagggga agaaatcaaa agtcaaatac ttctcctaag
    48061 aatgcagata ttatagacta caaacatgca actgggtttg aggcattagt tggatatttg
    48121 tatttaaata atgaaataga gagacttcaa tatattatca ataaaggaat caaaattatt
    48181 gaaagagata tgtaaatatt aaagagtgtt gtcttaataa taagtaataa ggcaacactt
    48241 tttttataac taatgtataa taaatatata gaaattagta atgagaaagg ataaaaagtt
    48301 atgaaagtaa aattaatatc acatacacct gaaccagaaa aagtaatagc tatggcagct
    48361 aagctatgtt attcaccagt tgggacagac gaaatagaaa aagacttaac agatgaaagt
    48421 atagagaaat ttttaaatat gttattaagt ataggtcatg ggtcaatatt agaacatgct
    48481 tcatttacat tttcaataga aggtatttca agggcatgtt cacatcaaat tgttagacat
    48541 cgtatagcta gtttttcaca acaaagtcaa agatatgtta aattagaaca atttgagtac
    48601 ataattcctc cagaaataga aaaaatagaa aaggcaaaag agttatttat agactccatg
    48661 aaaaaagacc aagagaatta tgataagtta gttgaaatat tgtttgaaaa tcattataat
    48721 gacctaataa aaaacggtaa aaatgagaaa acagctaaaa gacaagctga gaagaaagca
    48781 atagaagatg ctagatacgt atttccaaat gcatgtgaaa caaagatggt atttactata
    48841 aatgctagaa gtttgtttaa tttctttgaa catagatgct gtgaaagagc acagtgggaa
    48901 ataagaaatt tagctgtgga gatgttaaga gaggttaaaa aggttgctcc tatattattt
    48961 aaaaagactg gtccaagttg tgtaaatgga agttgtccag aaggaactat gacttgtgga
    49021 gatatagttc aagttagaga aaaatttaag gccttgtagg aggtgaataa tttggcgagt
    49081 atagaaggaa gaaatccggt aatagaagca ataaaaagcg acagagaaat agataaaata
    49141 ttaattgcaa attcagctaa agaaggttca attaagaaaa taataggaat ggctaaagat
    49201 aagaatataa ttattcagta tgttgataaa cataagttag atgaagtaag tacaagtcat
    49261 tcacatcaag gtgtaatagc ttatgcaagt gagtataagt attatgaatt agatgagcta
    49321 atagatttag caaagaataa agatgaagac ccatttttca ttatattaga tgagataaca
    49381 gacccacata atttgggttc aataataagg acagcagatg cagtaggagc acatggagtt
    49441 atcattccaa aaagaaggtc tgtacacata actcctgtag ttgccaaagc atctgctgga
    49501 gcagtagaat atatgccagt ttgcaaggta accaatatag ttaatacaat aaaaagactc
    49561 aaagaagaag ggctgtggat tgctgcagct gatatggatg gagaaacttt ttataaacaa
    49621 aatcttactg gtcctcttgg tgtagtaatt ggaagtgaag gttttggaat atctagattg
    49681 gttaaacaaa actgtgactt tatagtaaaa atgccaatga taggtaatgt tacatcttta
    49741 aatgcatctg tagcaggtgg aatactttta tatgagatat tcagacaaag actggataaa
    49801 agtaaatagg catgaaaaaa aatataaatc attacttaat aatagatggc tacaatatta
    49861 taaatgcgtg ggacaatttg aaagaactgg ctaaagagga cttagaagat tctcgagaaa
    49921 agcttattga tgacattata gaattttctg agtttatggg ttataagact ataatagttt
    49981 ttgatgctta taatgttaaa aattctcgag aaaaagttga aaaaagaaaa cacataacaa
    50041 tagtttatac aagagaacac caaacagcag atagttatat agagaaattt ataacttctt
    50101 tatcaaagta tgatgatgta aaagtagcca ctaatgacta tgcagaacag caaatgattt
    50161 taggaaaagg tgctactaga atgtctgcaa gagaattaaa gttagagtta gatcgttcca
    50221 agaataaaat gaaggaaaaa aatatcggtt tgaggaaaaa aattcagcga aattggttgg
    50281 aggaaaggtt agataaagaa acattgtcga aacttgagaa cattcgtaga aagcgttgaa
    50341 attactaggt tctttagact ataatatacc tataaatttg ttttatatat gggggagatt
    50401 tacatgttgg tagcaaaaga aaaaagttat gagttagtgg acaattgtca gcaggatgag
    50461 tataacattg tattaagagc aagtgaaggg gataaaatag cactagagta cattattact
    50521 aaatacagaa attttgtaaa ggcaaaagca aagtcatatt ttttaattgg agcagataaa
    50581 gaagatataa tacaagaagg aatgataggc ctttataagg cagttagaga ttttgatgga
    50641 agtaagacca attcatttaa atgttttgcg gagatatgta taacaagaca aataataact
    50701 gctataaaaa ctgcaacaag acaaaaacat atacctttaa attcatatgt ttctttgaat
    50761 aaaccaatat atgatgaaga gtcagacaga acacttttag atattatagc tacaagtata
    50821 gtaacagatc cagaagagct tataattagt aaagaagagt taaaaaatat agaatcaaaa
    50881 atgaacgaat tgttaagtga cttggaatta gaagtactag aattatattt aaatggaaag
    50941 tcataccagt ttatagcgga caagcttaaa agagatgtaa agtcaataga taacgcttta
    51001 caaagggtta agagaaaatt agaaaaacat cttgaaaaca gaaatgatta atagtaaaat
    51061 acttaccata agcttattgt gagcaaaatt aatttatatc gaaagataag ggaggaaatt
    51121 caaaatggct aaagctaaat acgaaagaac aaaacctcat gttaatatag ggacaatagg
    51181 acacgtagac cacggtaaaa ctacattaac agcagcaata acaaaaacat tatatgacag
    51241 atatcaatta ggagaagcag tagatttcgc aaacatagat aaagctccag aagaaagaga
    51301 aagaggaatc acaatatcaa cagcacacgt tgagtatgaa acaccaaata gacactatgc
    51361 acacgttgac tgcccaggac atgctgacta cgttaagaac atgataacag gagcagcaca
    51421 aatggacgga gcaatattag tttgttcagc aacagatgga ccaatgccac aaacaagaga
    51481 gcatatacta ttatcaagac aagttggagt accatatata gtagtattct taaacaaatg
    51541 tgacatggta gatgatgaag agttattaga gttagtagag atggaagtaa gagatttatt
    51601 aacagaatat gatttcccag gagatgacac tccaatagta agaggttcag cattaat
//
LOCUS       DAMJYX010000017        51030 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJYX010000017 DAMJYX010000000
VERSION     DAMJYX010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.017"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            343..2577
                     /locus_tag="PC479_001105"
     CDS             343..2577
                     /locus_tag="PC479_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2803659.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2608..3453
                     /locus_tag="PC479_001106"
     CDS             2608..3453
                     /locus_tag="PC479_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2803660.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3693..5426
                     /locus_tag="PC479_001107"
     CDS             3693..5426
                     /locus_tag="PC479_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2803661.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5758..6238
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5824..5846
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(6768..6872)
                     /locus_tag="PC479_001108"
     CDS             complement(6768..6872)
                     /locus_tag="PC479_001108"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2803662.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7497..7742
                     /locus_tag="PC479_001109"
     CDS             7497..7742
                     /locus_tag="PC479_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803663.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8495..8641)
                     /locus_tag="PC479_001110"
     CDS             complement(8495..8641)
                     /locus_tag="PC479_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803664.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8674..9060
                     /locus_tag="PC479_001111"
     CDS             8674..9060
                     /locus_tag="PC479_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803665.1"
                     /translation="MNNNKFVAPNNVTLVERCYRYIKDRECSFSPNTIRNRKNMIKNQ
                     IEVFFKDMKLIDITPSILQSYINNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENI
                     CDFVTTPIKKVVQLLSFIQNKKLNYY"
     gene            9927..11282
                     /locus_tag="PC479_001112"
     CDS             9927..11282
                     /locus_tag="PC479_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2803666.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11400..12068)
                     /locus_tag="PC479_001113"
     CDS             complement(11400..12068)
                     /locus_tag="PC479_001113"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2803667.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12135..12563)
                     /locus_tag="PC479_001114"
     CDS             complement(12135..12563)
                     /locus_tag="PC479_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2803668.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12670..13263
                     /locus_tag="PC479_001115"
     CDS             12670..13263
                     /locus_tag="PC479_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2803669.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13382..14317
                     /locus_tag="PC479_001116"
     CDS             13382..14317
                     /locus_tag="PC479_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2803670.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14588..15532)
                     /locus_tag="PC479_001117"
     CDS             complement(14588..15532)
                     /locus_tag="PC479_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2803671.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15850..17280
                     /locus_tag="PC479_001118"
     CDS             15850..17280
                     /locus_tag="PC479_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803672.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17480..18064
                     /locus_tag="PC479_001119"
     CDS             17480..18064
                     /locus_tag="PC479_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2803673.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18168..18947)
                     /locus_tag="PC479_001120"
     CDS             complement(18168..18947)
                     /locus_tag="PC479_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2803674.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19005..20261)
                     /locus_tag="PC479_001121"
     CDS             complement(19005..20261)
                     /locus_tag="PC479_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2803675.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20519..21418
                     /locus_tag="PC479_001122"
     CDS             20519..21418
                     /locus_tag="PC479_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2803676.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21442..22890
                     /gene="ddlR"
                     /locus_tag="PC479_001123"
     CDS             21442..22890
                     /gene="ddlR"
                     /locus_tag="PC479_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2803677.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            22949..23755
                     /locus_tag="PC479_001124"
     CDS             22949..23755
                     /locus_tag="PC479_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2803678.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23781..25700
                     /locus_tag="PC479_001125"
     CDS             23781..25700
                     /locus_tag="PC479_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2803679.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(25942..26289)
                     /locus_tag="PC479_001126"
     CDS             complement(25942..26289)
                     /locus_tag="PC479_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2803680.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(26276..28204)
                     /locus_tag="PC479_001127"
     CDS             complement(26276..28204)
                     /locus_tag="PC479_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2803681.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(28616..30805)
                     /locus_tag="PC479_001128"
     CDS             complement(28616..30805)
                     /locus_tag="PC479_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2803682.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            31136..31867
                     /locus_tag="PC479_001129"
     CDS             31136..31867
                     /locus_tag="PC479_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2803683.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            31947..32582
                     /locus_tag="PC479_001130"
     CDS             31947..32582
                     /locus_tag="PC479_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2803684.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(32833..33426)
                     /locus_tag="PC479_001131"
     CDS             complement(32833..33426)
                     /locus_tag="PC479_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2803685.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(33497..34360)
                     /locus_tag="PC479_001132"
     CDS             complement(33497..34360)
                     /locus_tag="PC479_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2803686.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            34523..35692
                     /locus_tag="PC479_001133"
     CDS             34523..35692
                     /locus_tag="PC479_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2803687.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            35793..36269
                     /locus_tag="PC479_001134"
     CDS             35793..36269
                     /locus_tag="PC479_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2803688.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            36834..37628
                     /locus_tag="PC479_001135"
     CDS             36834..37628
                     /locus_tag="PC479_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2803689.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            37652..38095
                     /locus_tag="PC479_001136"
     CDS             37652..38095
                     /locus_tag="PC479_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2803690.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(38184..39470)
                     /locus_tag="PC479_001137"
     CDS             complement(38184..39470)
                     /locus_tag="PC479_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2803691.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(39688..40578)
                     /locus_tag="PC479_001138"
     CDS             complement(39688..40578)
                     /locus_tag="PC479_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2803692.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            40870..41214
                     /locus_tag="PC479_001139"
     CDS             40870..41214
                     /locus_tag="PC479_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2803693.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            complement(41373..43469)
                     /locus_tag="PC479_001140"
     CDS             complement(41373..43469)
                     /locus_tag="PC479_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2803694.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(43482..44642)
                     /locus_tag="PC479_001141"
     CDS             complement(43482..44642)
                     /locus_tag="PC479_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2803695.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            complement(44661..45728)
                     /locus_tag="PC479_001142"
     CDS             complement(44661..45728)
                     /locus_tag="PC479_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2803696.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            46080..46646
                     /locus_tag="PC479_001143"
     CDS             46080..46646
                     /locus_tag="PC479_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2803697.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(46860..47846)
                     /locus_tag="PC479_001144"
     CDS             complement(46860..47846)
                     /locus_tag="PC479_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2803698.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(47876..48571)
                     /gene="pxpB"
                     /locus_tag="PC479_001145"
     CDS             complement(47876..48571)
                     /gene="pxpB"
                     /locus_tag="PC479_001145"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2803699.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(48591..49811)
                     /locus_tag="PC479_001146"
     CDS             complement(48591..49811)
                     /locus_tag="PC479_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2803700.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(49840..50631)
                     /locus_tag="PC479_001147"
     CDS             complement(49840..50631)
                     /locus_tag="PC479_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2803701.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
BASE COUNT        18369 a         7700 c         6283 g        18678 t
ORIGIN      
        1 cctttgaaaa atcattaaac ttaaaattaa ttataaaaaa actctacctt actaactata
       61 cagctcacaa tctatctatt tatgaaaaaa tatgatataa ttgcattcaa aaaggaaatc
      121 tttcaatata ctagaattta agcattaact aattttatct aatattatta ctaaactaaa
      181 aaatatttgt atttgtagca ttggagcaaa taacttaatg tatattcatt ttaaatactt
      241 atttaaaatt tagcatttaa attatttaca aattaacatt taaatacttc tagcataaat
      301 acagttgtca tcaaaattta aagtatctta tggaggaata ccatgcaaaa tcataattat
      361 ataaaaatgt taaataaaaa ggtagaagta ttatctaagg aattagatag tagaaatttt
      421 aaattaaaaa ccttaaatga aatagatttt ttttctaaaa taccaaacag agaatttatg
      481 taccaacata ttgatgactg tatttcgaaa aatatacata taggaatcat gttattaaat
      541 atcgataact ttaaattatt taatgatata tatggatacc tagtcggaga aaatttatta
      601 attactctaa ctcaaaacct attgcacttt attccagaat caaagggaat agtcgcttat
      661 ttaaaaggag aaggtttttt aattgtactt ccaaatgtat caaataaaga gttaaataat
      721 atgggtgcaa ctattataga gagcgcccga gaattaaaaa taagacttcc taatgaaaaa
      781 gaaatatact ccaatgttac atttagtatc ggcagcacta tctggaatgg tgaaccaaac
      841 atgactactt tgatgctttt caaccaagtg tatagtgctt taaaaaaggc taaaagtgaa
      901 ggtaaaaata gatacattct atttgatttt aaagataata gtttttacaa taaattagaa
      961 aatgataagg agttcttatc agaaatatca catgttctac aaactaaaca aattgaagta
     1021 ttttaccaac cactttataa tataaaaaat aatactatag ttggatgtga ggcacttgca
     1081 agatggagac accctattaa aggacttctt actccagata aatttattcc tttatttgaa
     1141 cagtttggtc tgataacgga attagatttg catttatttg aagagaactg taaaaatata
     1201 agaagatgga ttgataacaa tgatgttttt gttccaattt gttgtaactt ttctcgtctt
     1261 cattttttta accctaattt tccaagtatg ctaaaaggta ttactgaaaa gtacagcgtc
     1321 tctacatcaa attttagcat tgaaataaca gaaaatatat taatagaaaa tacaaataca
     1381 attattcaac aactagctga acttcgttct tatggttttt ctgtatcaat ggacgatttt
     1441 ggttctggat attcatcttt tggtatgttg caaaatctac cttttgatat aatcaaaatt
     1501 gataggatat ttttatctca taatttaaat gattttaaaa atacaaccat tttgtactct
     1561 ataataaaca ttgcaagagc tcttgggatg cttacagtat gtgaaggtgt tgaaacagaa
     1621 caacatgtag aatttattaa aagtataaat tgtgatatcg cccaaggtta ttattatgca
     1681 aaacccatgg agtgcaaaga atttgaaaaa ttactatcta aagagaataa ttcagagaaa
     1741 atgtttataa aatattctga tataggaaaa tctttaatag agaagatatt tgatggattt
     1801 tttattatgc aagatatgaa aaaactaaga gctaatgtca ctgaagacat tcaatggttt
     1861 gatttttaca acaaaactga aattacatct tttcctaaag taatagagca ttttgaaaaa
     1921 catattaaag gtaaaaaatt ttctattctt tacaaaagta taaccccaca taatactgaa
     1981 tcagattcat tattaattag tggagaagcc gttttatcag atgaagcttc tgaacatcaa
     2041 aaatatagca atttctactt tagtgcaaat tgtataattt cagaaaataa attactctta
     2101 gcaaaattac atttggattt aattaaaact gctggctata taaatgaatt tgaatcatat
     2161 tcaataccta aaatgacaaa tacacatctt gatgaaagta atattttatc acaattttat
     2221 tcaatgttgc ctgtaggtat aattcgctac gatttatttg gtgatatgat tattacttat
     2281 atgaatgaag aaatgtttga tattataggt tatacaaaag aacagttttt tggtgagatg
     2341 ggcggaaatt taagagcaat tgttcatcca gatgatttag actttgttta taaaaaaagt
     2401 ttggacatga ttgacacaaa taaaattgaa ccatttttat atagattcat aaaacgagat
     2461 ggtcaaattg tcacagttat gtatacacaa tgtaaccttt ctgcaattga tggaaggtcg
     2521 attgcccagg gtatgtatgt agatgtaaat aaaataaaaa atttaacaaa aatctagtac
     2581 aaagtatttt attttggaga aattaatatg tttaaagaaa tttttttaag aacttttcca
     2641 ggatttctaa ttataagaga ttctaactac agaattatat ttataaatga taatttaaaa
     2701 aatttaatta aaagttatat gcaagataat ccacttggaa tgactaatat agaaatagct
     2761 aagaaactcc ctgataatat tgctaaattt tttacggatt ctcacaatat tcaattagat
     2821 tgggaaaaaa attatcctta tgataaaatt tcaaattgga ttttagagtt taaaaaagat
     2881 actacaagct attggaatgt tttagaatac aaagtagatg ttgatgaaaa aacctacata
     2941 attactatgg ccaatgatat aacaaaactc tatgaagaaa ataagagaaa tcttcactac
     3001 tcaattactg acccattaac tggagcatat aatagaaaat atttaaatga tagatttgac
     3061 atatttatag gagattatat tgtactaatt gacttagata atttcaaaat gataaatgac
     3121 tatgaaggtc ataatgtagg agacaagatt ttatgtgatt ttgtttcact tttaaataaa
     3181 gaattaataa attctacctc tattatacga ttaggaggag atgaatttat tgttatattc
     3241 tctagtgatg ttgacaaaaa ttatgtttat tcacagctag aagccttaag agaaaatttt
     3301 ttaaaagtat tctctaagta taagtatctt tcatttagtt atggtgttga tacagtaaaa
     3361 agaaatttaa aattgacaat agttgaattg gataaaaaaa tgtataaaaa caaggagaaa
     3421 aacaagaaaa aatttgataa aaatgattat taacattgtt attttaaaat aaattaaaat
     3481 tatctttctt tttttaaact tgtacattag tttataaata tgtatctata caagatattg
     3541 acattaagta ctgaagcata tattgtttca gttctttttt gttaaaatta acttatttaa
     3601 caaagatatc atagtttaac aaagctttta atttaaatat attgatgtat aatataatta
     3661 aaatataata gcaaaaagaa aggaggaata tcatgagcat actcctaaaa aaagcaccca
     3721 aattagcaaa acatattata acaagttttt atataaatcg agatatcaat gaagtattaa
     3781 aatacctttg tgaaaatgtt acatggattg gaccaggaga acaggaattt ctcacttctt
     3841 ttaatgaaat aaaaaattat ttttatgcag gtcaagatga aattccttct tgtgatataa
     3901 ataatgatat atttgaaact gtaagtgaag atgagaatag atgtatggta ttaggaagat
     3961 atactgttcg aactaaagaa aatgctcaaa tgattttaga ggtaaatcaa cgttgtacat
     4021 ttgagataat tgaggataga ggaaaattat taataaagca catgcacata tcaaaccctt
     4081 atggtgaaat gcaacttgat gaatactttc ccactaaaat tggaactcaa agttatgatt
     4141 atttgcaaag attactaaaa gaaaaaacag aagttattga aatgattaca aataatatca
     4201 atggtggctt aaaaggtagc aatgatgata gtacatactc atttttctat gtcaatgagg
     4261 gattgcccaa aatattagga tacacatatg atgagttcat ggaaatgagt ggtggttcag
     4321 cagtgggtgc tgtatatcca ccagacttac ctaaagcact agaagattgt gaacgatgtt
     4381 ttgcaaaagg acctacatat tcatctgaat atagaatccg aaaaaaagat ggaacattaa
     4441 tgtgggtatt agattctgga atgaaatcgt taaatagtga tggcatagta aaaattaata
     4501 gtattattac tgacataacc caactaaaaa acatagaatc tgaactaaaa ttagaaagag
     4561 agcgatatcg tattgctttg caaaatatta cagatatcat gtttgagtat gatattgaaa
     4621 acgataccct tataaaatat caacgtgtag aaattgacaa aaaaattgaa ttagaaaatt
     4681 ttgaaacaaa aaattactcc aaactattag aatctggtaa aattattcac cttgatgata
     4741 ttggaaaact actagaagta cttcatggaa atcttcgtga aacaattgag ataagacaag
     4801 taaattcatt aactaaaaat gaatggagat ggattcgtgt tcaatgttca gttatatatg
     4861 atagtgacca taaccctatc aaaacaatag gcgttttaaa agacataact gaagataaat
     4921 ctaaattaga aagtttaatt aatcaggctc aacgtgaccc actaacacaa ttatataatc
     4981 aaagagtatc acaaaatctt attgaagagt atctatgtag ttctgattcc aaaaataatg
     5041 atgctctatt agttattgat attgatgatt ttaaaactgt aaatgatact tttgggcatc
     5101 tagagggaaa tgaagtttta gttgctgtat caaaaatact attacataac acacatgata
     5161 aagacatagt tgctcgaatt ggtggagatg aatttacaat atttataaaa tcattaacaa
     5221 aggatttaat aataaaaatt actaatgata ttttaaatga tgcgagtaaa ataaaagtaa
     5281 aggacaatca taaaataaca ttaagtattg gtattgcctt cactgatgat tcaactaaat
     5341 tatataaaga tttattttct aaagctgata aagctcttta tctatcaaaa gcagatggca
     5401 aaaattgtta tagtacatac gaataataaa gctatttata cgaccaagaa tgaaccttta
     5461 aatttaattt catagtctaa caatcaaaga gttgaagcta atcttatagc tagtcaacta
     5521 gttcctagaa aaaatttaaa caatatattt attgatactg ctcatttttc taaagagcaa
     5581 atttatataa aaaattaaac ttttatatac atacagatac taatgataaa cagttatcat
     5641 tagtatcttc tatcttaaaa caaaatgata atttatctat ataaaaaagt gctttgttga
     5701 cagagttcac ttttttatat aggtacagaa tttaagtata aatacatgtt ttattctata
     5761 cattttagtt aatgtaaaat ttcttgattg tactctctaa cttttcaaca ttatactatt
     5821 tatataccac ttagttaata taaaactata aaagtgttgc aattactggg ttttataaag
     5881 ttaatttaca taccacttag ttaatataaa actttagcac cttatttatc tatagagcta
     5941 cagaaagtat ttacatacca cttagttaat ataaaacatg tatttatttg ttcttgtact
     6001 attgttttac tctatttaca taccacttag ttaatataaa acaatatcaa aacaaataaa
     6061 aatttttaat aaatcattat ttacatacca cttagctaat ataaaacatt ctggtactgt
     6121 tgctatttat gttccgtatt cagaatttac ataccactta gttaatataa aactttggag
     6181 ttgttacaga ttatttgcct tatctctcta tttacatacc acttagttaa tataaaaccc
     6241 ccaaataaac tgggcatttt caatacttac acatatgcaa ctctcttaaa tttgcagtga
     6301 accatgagta gtgcaaatga taaagcttat catataccct caacacctta tatttcaagc
     6361 gttaagctct actttgtaac aaatatcgct cactgcaaaa ctatttacat ttttattata
     6421 tcataaatat attatttttg aatatctata ccaatttgtg gtataataaa agcaagaaga
     6481 actacaatct atttagacgt agagtgaagt tcaaatacta atgtttattc tttttgaact
     6541 tgatttttag ttcaaaacta aagtcactcc tgcctgtgtg aactataccc gctgttttat
     6601 acagttaaaa catgtaaaaa tagttataaa gtactagtgt aactcattag tactcttttt
     6661 attatattca tataattaat atctatattt taaagcttac tatctaaaaa agagcacgtc
     6721 aaataaaacc ctagatattt aattttttta gagatactta aaataaatta tgctctctct
     6781 gtacttaaat atttcctaaa ttcaaatgga gacatagaat ttaatctatt ttgatatcta
     6841 aaattattat agtagtatat ataatcatct attgctttaa ctaattattc atatgtatca
     6901 aatttcctta aatagtacat ttctaccttt aaaattcccc aaaatccttc cataggagca
     6961 ttgtcaaggc aacgaccaac tcttgtataa tatatgtata tctaacacca ttaagaaata
     7021 tatattttat aatatatatt tcttcattta atgcacttag aaaagcaaag aaaatacaga
     7081 ttagtacact ttttagagaa aaataaaatt aatacatcta aaatattaaa aaatacttta
     7141 gataatctaa agacacatct caaagttttt aactaaatta taaaactaat tacaaatgct
     7201 atgtaacgat aataaataaa aaatttactt cgcttaagcg acgtgtcgtt gaaatatttc
     7261 atgttgccaa ctgattcata agtcgataag aaaagaacac taattattta gagttctttt
     7321 cttagctaca agtggattat aatactttta aatctgtgag aaaatcatat ataataatga
     7381 tatgaacaag taaagaaaat acaaaaatga caaaattata aataaaatat tataaaatca
     7441 taaggtcata attgttatat tgtagaaaca aagtaaagag agagggtgga gaataaatga
     7501 atcaaaaaaa aataaagatt agtattaaaa tggtaattat aggtatattt tctgtaatta
     7561 ttgcttttgt attatccaga ttattcgttg agtatattga tgaattatta aggtctaatt
     7621 ggcaatatca gctatttgaa agtgagagtt catatataat atttgccaat tgtattatgt
     7681 tctcgaatat attattagaa atatttttaa tatatatttg tagaaaattt agaaaaatct
     7741 aatatggtca acgtatttcg aatcgaatac ttcatgatta tttagtctat tgtgctagtt
     7801 aaagtttaat tcccatataa agtaaattga ttttagtaaa acaaatcacc cctaagggtt
     7861 agtttatgga taagtcatcc aaatattaac tctttaattt ttgctataga ttcatctttg
     7921 ctcataactt tacttataaa aaatgaaata ttataagcta gaacttttat tctaattcta
     7981 gtcataaatc ctagcataga gttacttttt ttatattata aataaaacta tacaacaaag
     8041 ataatattta gtaaaaataa ctttcatttt aactaaactt aaaattcaat tactctctaa
     8101 attgacgttt aggaattagt tcaaaataaa aaagagtgta tatattgaaa ttactacatt
     8161 tcaatatata tactcttttt aaatttctca gttttccatg gaactttact ttatttattt
     8221 ctgtattagt tgtatttttt atgcattttt ctttaaaatc atattttctg attcaactct
     8281 tatattataa aatttcttta ctaaattatt ctctttttct atatcttcat atatattttt
     8341 taatcgcctt ttacctaatc ttttatctaa cattactaat gatttaacta atatattata
     8401 tgattttatc gcatcttcta ttgagatatt taaacatata tttactgctt cataaaaatc
     8461 aaattcatta aaaaatccat ttttatttac aatcttatat ccctttgcat ctctttctaa
     8521 atactcttcc taaatactct tcaaaacttt catcatctaa tctaaatcct atatcactat
     8581 gaaaaatagg ttttgcattt ggatatagtt ctattgtttt atcaaaagtt tcgaatacca
     8641 agttattatt attcgatttt ccaatagtcc actataaaca ataataagtt tgttgctcca
     8701 aataatgtaa cacttgtaga aagatgttac agatatataa aagatagaga atgtagtttt
     8761 tctcctaata ctattagaaa tagaaaaaat atgattaaaa atcaaattga agttttcttt
     8821 aaagatatga aattaataga tataacacct tctattttac agtcttatat aaataacata
     8881 tacaatgagc acatgttaaa ttcaataaaa aaccaagtcg attttataaa atcagtattg
     8941 aaagaatctt atagattaaa agaaatatca gaaaatattt gtgattttgt tacaacccca
     9001 atcaaaaaag tagttcaact tctaagcttt atacaaaaca aaaagctcaa ttattattag
     9061 aaaaatcaaa aaatattcct ataggtatac caattttttt gatgctaact cttggattac
     9121 gttttggtga agctgtatca cttatatggt cagatgtaga ccttgataaa aaatctcaca
     9181 tgtaaatcaa acattagttt atgtatacaa taaaataatc ttcaaagacc ctaaaacccc
     9241 tatatcaaaa agaaaattat ttgctcctga tgaacttata agtttgctta aaggggaaaa
     9301 attaaaacaa aataagttaa aattacaggg tatattgaaa aatgaatttg atttgatatg
     9361 cttaaataaa agattcaacc cgtgagttca accaactttt gttgttcatt ttaaaaaact
     9421 attaagagat aataatccta gagatataaa agtacatgaa ttaatacatg caaatgcaac
     9481 tttgatatta ctttcaggta ctaacataaa agctatttat aaaatattag tacatgcaga
     9541 tataaaaata gctatgaata tatattccat attctcactt attagatgaa atgaataaag
     9601 aaacatctga aaatataagc aaaatactat tcaaataatg ttcagtttta tgtcaattaa
     9661 atgtcactca aagtgctttt ttttcatttg tcagttaaat gtcacttaaa gtaccttttt
     9721 cctatttgtc agttaaatgt cagtaatata taaataagat atatttataa gacccttaaa
     9781 atcactatta tttaataaaa tttataatta tatactattt gctgttgaca ttggtattat
     9841 accatagttt atcatttata ttagaaataa aattattaat taatagtttc gtaattttat
     9901 aatagattta cagggagtga tagtaaatgg atacaaaaat atctataaac gaaaacatga
     9961 gtctaggtaa aagatttttt aaatacctag caccatctgt agttgcaatg tgggttttct
    10021 cactatatac aatggttgat ggaatatttg taagtaaagg agttggagaa ttagctttag
    10081 ctgctgtcaa tatatcaatg ccttttatta attttatatt tgcagtttct ctattatttt
    10141 caactggtgc atctaccata attgcaatct atctggggaa aaaagatatt aaatctgcta
    10201 acgaagtctt ttcatttaac ttagtatcaa taattatatt atcaataatt atattagcta
    10261 taactttttt taatttagat agattagcac tatttttagg agctacagaa tctacaattg
    10321 gtatggttaa agattattta ggaataataa tattttttaa cggattcttt atagtgtctt
    10381 attctttaga agttataata aaaactgatg gctttccaat attagctact gttggtgtaa
    10441 taatatctgc tcttacaaac atcatacttg attacttgtt tgtaattgaa tttggatggg
    10501 gtgtaaaagg tgctggtatt gcaactggtt tgtctcaagt cttttctact attttcttct
    10561 taatacattt tttaagaaaa aactctactt tgaatttttc aaaatttaga attgatttta
    10621 agacacttag aaaaattgta tttataggtt ttcctgattc aactactgaa ttaagttgtg
    10681 gtatagttgt tttacttttt aacttaagtc ttactaaata tattggagaa aatgctctta
    10741 tttattatag tgtcataaat tatataaata cattagtact tatgactatg atgggtataa
    10801 ctcaaggtat gcaaccactt actagctttt attatggtgc aggaaatata gataatgtaa
    10861 aaaaattatt aaaaatgggg ataaaagcca caataatagc atcagttgct gtatttgcaa
    10921 tttgtatggc tttttcagga ccaatagtct ctttatttat acatccagaa gaaacaatgc
    10981 tattcaatga aggagttcgt gtatttaaaa tattctctat atctttctta ttggtaggaa
    11041 ttaatgtaat aatttctgga ttctttgtat cagtagaaaa accatcaatt tctactgtta
    11101 tatctcttgg tagagggctt gtaatagttg ttttaagtct aatttcaatg atactaatat
    11161 ttggtggaca aggaatatgg atgactacta ttgtttctga gtttatatgt cttattttat
    11221 cattagtttt tctaaaaaag aatttttcta cattagactc taatctaaat aaggttgctt
    11281 agatgataag agtgacaata tatgaaaata tctaaataaa aaatacctat tatacaatct
    11341 tgcaaatatt aaacctttgc aaaaattagg attatataat aggtatttta tttatgtaat
    11401 tattttattt ctaaatatcc atagccatct tttattaaag ttccttttac ttcatattct
    11461 acaggttttt taaatattgg accatctacg acaaatgtat gatactctcc atgttctcca
    11521 catgggtctg caccagcatt ttttatcttc tccacaagtt ctctagttaa tttttttcct
    11581 aaaaattcct ctcctaagtg ctttaagttt acttttgtta caacactaca aaatccactg
    11641 tctataaatt catatactaa ctcctctcta ctctcttgcc ataatggtaa atctactttc
    11701 ataccaactg cttcacatct atcagtatcc cattttctat gactttctat atctatatct
    11761 ccatatgcac atatagttgc tcccatgttt ttagctttta ttaaagccct ctcaaagctc
    11821 tcttcatatc cttctacacc acactcagca actaataaag gtaatcctat attgctactt
    11881 acttgcttaa gcatattata ttctaaatta tgtgtccatg actttccatg ttcttcactt
    11941 atagttgtca agagtgccac aggcttatat ccttttttca acattcgatt taatgctaat
    12001 atactatcct taccaccact aaatgacatt acaaattttt catcagccaa ttttaacacc
    12061 tctttcatct aactttgttt tattaacata attataccaa atttttgagt tgcatagcaa
    12121 attatattaa attgttaatt cactataaag aatgtcccta atattaaaag tataacccct
    12181 atgtatttgt ataaatgcat tgtttcttgt cccaaaatta cgctatcaat aaaaaatcca
    12241 actaaaactt gggatacaag agttaatata agtgtatttg atactcctaa atttggaatt
    12301 gcctttactg taaaatatat tattagacct ccaaatactc ctcctaataa caacttataa
    12361 ttaaaaccat ttatagatac tagacacttt aagtcacctg taactattat acttgccaaa
    12421 aaaaatattg ctcctacagt taagcttata aaggtggcca ctaatgctgt tgtacttttt
    12481 cctagctctc catttataaa agcttcaaga gctgtagaac cacctgctaa taatgcaaat
    12541 attattgccc ataattttgt catggacaaa cctccttcta tataattcta ttaatagaag
    12601 tttatgatac aacctattaa ttaattacta agtatagttt aatttttata cactaggctt
    12661 tatttggata tgaatttctg ttataattgt tctatcgatt ttggaggtga ttttatgtac
    12721 acaaatttta atatcgattt taataacttt aataaaaaag aaaatgccat taaatttatg
    12781 ttaatgggag tattactcat tatattagga ttattatgcc ttacatttaa gacattaggt
    12841 ataaaattaa tttcttggac ttttggtatt gcacttttat tctttgcata tttaaattta
    12901 aaaaatatta atgaactaaa aagatatgct actaaagaag aaattaaacc ctctattaat
    12961 atccaatggg ttctaataat tgcatgtata ttactttttg tatttcctca aaaaatacaa
    13021 agcatatttt cattattttt aggattttat ttaatcttta atcaactagt agcacttgta
    13081 aatagtaaga ataatcctta ttctaaattt acaacttgga atatagttaa gattctattt
    13141 ggtatatgcc ttatattatc tccacttttt ttatctagat ttatagtatc gataatgtct
    13201 tttttcataa tattatttgg actagttcta tttttctctg gaaatacagc gagaaaatat
    13261 taattatttt ctcgctgtat ttttatatta cttaaaatag aatttgttta agttatctat
    13321 ttgtggtaat atgttttttg tacaagctca gcgtacaatt ttattagaaa taggtgattt
    13381 tttggataaa tttaaatatg cttttgataa taaaagatat catacttgga attactatct
    13441 tagaaatact tttggagaaa aagtttttaa ggtatctata aatgcaggat ttacttgccc
    13501 aaacattgat ggaagtcttg gttatggcgg atgtacctat tgtagcaaag aaggctcagg
    13561 tgattttgct ggaaacccta aagataatct tatcagtcaa ttttataata taaaagaaat
    13621 gatgttaaaa aaatggcctc atgcaaagta tattggctac tttcaagcct atacaaatac
    13681 gtatgctcct cttgaagttt taaaagaaaa atatgagact atattagaac ttgaagatgt
    13741 cataggtctt tcaatatcca caagacctga ctgtttgcca gatgatgttg tagaatactt
    13801 gtctgaacta aataaaagaa ccaatttatg ggttgaactt ggacttcaaa caattcatga
    13861 ttctacatct aaaattataa atagaggtca tgattataaa acatttgttg aaggtgttga
    13921 aaaattaaag tctaaaaata taaaggttgt cgtacacatt ataaatggtc ttcctggtga
    13981 agattataat atgatgatgg aaacagcaaa ggcagttggt aaaatgggtg tagacggtat
    14041 aaaaatccat ctattgcatg ttataaaaga tactcctatg gaaaagatgt tacaaaatgg
    14101 tatgttaact cttatggaac aagatgaata tatcaatctt gtctgtgacc aattagaaat
    14161 acttcctgaa actatgataa tccatagact cactggtgat ggtaagaggg atgaactagt
    14221 aggtcctata tggagcctaa aaaaatggga aattttaaat aaaattgatg atactttaaa
    14281 ggctagaaat tcttatcagg gctgtaagtt tgtttaatac aaaataataa ctatagaaat
    14341 ggttatacaa atatagtcct ttctatagtt atttagtatt tacaaaacaa aatattaatt
    14401 tgtacattta tacaatatat aataaaaaat agatagtcta aactgtatct acttatattt
    14461 tatttgtact gtccacttta ttctataaaa tttataaata gttatttttt aataccaaat
    14521 ataatttggt ataatttttg ttggaatcta caatgtatac caataaatcc tttttaagta
    14581 tataattcta aatactttca gttttttcaa tctgctttaa cttgttggac ttataaaata
    14641 ttcttatttc atcataagaa agattaagtg ctactactgc agatattaac atcaacatca
    14701 ctccaaataa cttccctaag gaaaaacttt ctcctaatat agtgcaacca attattactg
    14761 aaccaactaa ttctactgat gccacaacac ctgcaactga caactctaca cctttagata
    14821 tacctgctgc ataaaaaata tatgcaagtg cagctggaac aatgcctaac attagcatac
    14881 aagataaaac atctaaatcc ttagcataat ttaagatttc ccaaggtctt gaagaaggaa
    14941 tcataaatat agctccaaat aagaaactgt atactaatat tgttgaactt gaaaattcct
    15001 ttaaagcatt tttactaata gttggcataa gtgcatatgc tattgctgat aatactccaa
    15061 gtaaaagccc aaaagcgttt aaaccttgaa aatccaaacg tccaccagtc actgccataa
    15121 ttgcaccaat aaaacaaaga ataagtgaaa ataatttgct tctagtaata ttttctttat
    15181 aacataattt tgaaaatatt gctaaaaaca gtggtgatgt atatagtaaa actgctgcaa
    15241 ttgatacacc cgcaatgtca attgctttaa aataacataa attaaacatt gcctgacata
    15301 taattcctat tattactgaa tatataattc cttttttgtt tatctttaag atttgtggtg
    15361 tccttataga tgaatatacg cctaaaacta taaatccaaa aaacaatctt gtaaatgcta
    15421 cttgttctga tgtcagtcca tttcccatta aaaactttcc aaataatcca agtgtagccc
    15481 ataataaccc tgctattgct ataaatacat accctttact tttttctccc attttaaatt
    15541 cccttctatt tttatttagt caatttacac tcttcttttt aagcaaaaac tatgccatct
    15601 taaatcaata tatatcgact ctaaaaccct agtctttcca ataatactaa taaattttca
    15661 atttttttat atttattttt cgcttacttt aagttgattt agacttattt taattgtaaa
    15721 atgagttata tataacttat ttaaagttaa aatagactga aacttatata taaatatcaa
    15781 attttaagta tattaatcaa ttaaacctat tttaatatat cattaaaaat ttaggaggaa
    15841 aatataaaaa tgaatttaga tttggaattt tatcaaaaaa tattagaagc ttcccatgat
    15901 gaaatatgtg tttctgatga taaaggaata ataatatatt gtaataaagc atttgaagaa
    15961 aattatggtc taaaaaaaga agatattttg ggaaaaaatg tttctttttt agaagactct
    16021 ggatattcaa caaaaagccc cataccaatt gtattaaaaa caaagactaa attttcactg
    16081 gaacaagata ctcaaacagg gaaaaagctt atcataactg ctacccctat atttgatgaa
    16141 aatggacact tagaatttac tgttgaaaat tgtagagata taactgaact taataatata
    16201 aaaaataaac ttgaagacac aaaaaagcaa gtaaaaaaat ataaatctga agtagaaaca
    16261 ctatacagaa ctgcacttag aattgaagat actgtaatta tggacggaat agttatgaga
    16321 ccaatcataa acacagtaaa tcatgtttct aaaacggatg taagtgtact tttattgggt
    16381 gaatctggaa caggaaaaag ctcacttgct agatatatac accacaacag caatagatct
    16441 aatggcccat ttataacaat aaattgtgca actatatctc cacaacttct tgaatctgaa
    16501 ctttttggat atacttcagg tgcttttaca ggtgcaagta ctaaaggtaa agttgggctc
    16561 gtagaattag ctaatggagg aactcttttt ttagatgaaa taggagatat accacaaaat
    16621 ctacaggcaa aatttttgca attaatacag gatagaactt ttactccagt tgggagctta
    16681 aaaaataaaa aggtcgatat aagaattata tctgctacaa atgttgattt agtttcaaaa
    16741 gtaaaagaaa aaaagtttag agaagattta tactatagat taaatgttat agaaataaaa
    16801 ttgcctcctc ttagagaaag aagggataat ctagtggaaa ttataaaata ttattttaat
    16861 aggtactcaa gtgacttcaa cctaaataaa actatttcta aggaagctat ggatgctatt
    16921 gcaaattata gattcccagg caatataaga gaattacaaa atataattca aaaaatcttg
    16981 cttacttgta cagataatca tataacaata gatgacttac caaacattct tacaaaaaat
    17041 atacatatta ccaataatgg aaataaaaca catatatccc aaattaataa ggttattata
    17101 tctgactcaa aatctactaa ttataaaaat aaaaattttg acactttaat taaagagtat
    17161 gaaaaaaata taattttgga tgcttatgaa aaatttggta gttcttataa agtagccaaa
    17221 caccttgaaa tctcacagtc taaagccaat aggttaatta gaaagtatac taatacttaa
    17281 ttttaaattt gataaataaa aacttaacac tatttacata ttgtaaattt ggccatttac
    17341 tgttattatg aatatggttg aaattattta aataaatact gttgacttag tttcatatat
    17401 ttaccattaa acttttatca taaatatcat atcataataa tatatttaat aagtctaata
    17461 aactatacga ggtgtaaata tgttaatgaa tacccatttt attatggcaa aatcaatact
    17521 tgataatata gatgaaaata aaacattctt tataagcgaa aaaaatttta tatatggcaa
    17581 tataaagcct gatgcttttt ctaaatataa attaaaaaaa cattatttag atgagtcatt
    17641 taatatgatt gtttctaaaa taaactatct atgcagtctt aatatagata gtctttctaa
    17701 atacttttca gtaagtagac ttagtcagga actaggagtc atatgtcatt ttttatgtga
    17761 ttttttctgt gtagctcaca gtgaacgttg ggaatttaag catagcttaa ataaacatgt
    17821 tacttatgaa agagaacttg gagcatttgc aaaagatgtt gatttatcta aaattaaatg
    17881 tggtactata aatagtagtt ttgagaagtt ttttactaaa ctttatagtg attacaaaaa
    17941 gaaaactgat tatatgaatg atttaaaatt ttcagcttat acttgtaata gtgtcataaa
    18001 ttatatactt gactctattt tatctaatac aataaaatca tatcatatag caaattgcgg
    18061 ttaacttaag aatttttaaa cttatataaa cttataattg aatataatta aaatgactgc
    18121 tctatagaat ataattttat acttctctag gacagtcatt ttttatacta actatttatc
    18181 tccattataa catctatggc actttttaca ctatctacta cataatttgc actttgcttt
    18241 acatcttccc ttgccttaga cattgcaaaa ctatatttaa atccttctaa catgcaaata
    18301 tcatttggag aatccccaat agtagcaact tcatctggac ttacatttaa cgtttttatt
    18361 acatgctcta tacttcctct ttttgacaca cttttaggaa ctatatttaa agtatatgtt
    18421 ccagaaaaat atatttctaa gacttcacta aactcacttt ttagataaag ttctattctg
    18481 gataactctt ccaaattttc agaacatacg cctatatttc ctataagatt atcaaaacta
    18541 cttctttttt caaaatctgg gtcaactata aagtcactag aataatgtct aaaagcagtg
    18601 gtatcactac ccacaattac atcaccctta tatttaaaat aaagttgtgc atcacttgac
    18661 tcaaatctat caatgacttt ttttactaaa ttatcatcta tcgttctttt aaaaattaca
    18721 ttataatctt tatcatatat aaatgaacca ttttcacata catagtatcc attcatatct
    18781 aaactatctt tgataatatt atgtgcttgt ttaaaaattc tacctgtagc aacattaaac
    18841 tgtactccct ttttttctat ttcttcaatt gctactatat cttcttttgt tatggttcca
    18901 ttttcatata gagttccatc taaatcacaa aatatatgtt ttatcataaa ttaaaagcct
    18961 ccaaaaacaa ttttatattt tactaatact ttaatcaaaa taacttaaag cttttctaat
    19021 tgtattctca attcattaag caaatctcta gtagctctta atcttatctt tcttctatcc
    19081 ccattaaata tatttttctt tactattgtt tttccattta tatatattgc agtataaacc
    19141 agtcccacag gcttctcttt ggttcctcca ccaggtccag ctactccagt tgtagatata
    19201 cctatattag ctttaagtcc tttagcaatg ccctcagcca tttctatagc tgtttgctca
    19261 cttactgctc caaactcttc caaagtttct ctttttactc ctaatctttt cattttagaa
    19321 tcattagaat atgttacaca accttcaaga aatacagagg atatacctgg atagtttata
    19381 agagatgaag aaaccaaacc tccagtacaa gattcagcca ctgctattgt tagatttttt
    19441 tctacaagta gcttagaaac agcatcttct aaagcagtct ccccttcaga tatggaaata
    19501 tctccttcag tatatacata tttaccaact ctacttttta ttttttcttc tactggtttt
    19561 attaatttaa aagcttctct ttcattttca gcctttgcag ttattcttat tgtaacttct
    19621 aattcttttg catataaagc aactgttgga ttagtctgct cattaattat atcgagaatt
    19681 tctaattcta gatttgactc acctattcca tataatctta aagtcttaga tactagcatt
    19741 tcatttgtga attgttgtaa gtaaggtttg acactctcat taaacatagc tttcatttct
    19801 cttggaggtc caggcaatac tattataaat ttaccatctt tttttaaaat tgcccctgga
    19861 gctgtaccat tattattttt tagtactata gcatcttttg gaaagtacac ttgtttcata
    19921 ttattttcgg ccattttttt ccctgtttta acaaagaaag attccaaaac ctctagtgaa
    19981 ggtttatgta gttctaattt ctgaccaaag tattctgcag cagtttcttt tgtcatatca
    20041 tcaccagttg gcccaagacc acctgtagta attacaaaat cacttctctt taagctttca
    20101 tcaaaacatt ctaaaagtct ttgtttgtta tctccaacag tactctgatg gtatacttct
    20161 attcccaatg atgcaagttc tttagctaaa aattgagaat ttgtatttac tatatctccc
    20221 aataatattt ctgttcctac acttattatt tcagctttca aaatttatca ctccttgatt
    20281 tatagccatt aagactagtc ttaagttcat aacaataatt ttagcaaaaa taataacata
    20341 aataaagtta aaaaatcatt taaacatatc tttaaactag attattattc taaaaccaca
    20401 cataatataa acttcgtata tagtggtact ttcatcctca actgttaatt catataatta
    20461 agtatgtaaa taaaaagttt actttaaaat aataaaaatt aaaataggag tgtataatat
    20521 gaaaatagca gttataatgg gtggaatatc atcagagaga gaagtttctt taaattcagg
    20581 aaaagagata tataataacc ttgataaaaa taaatatgaa gttgtaaagg taataataga
    20641 tgataaaaaa gatatattta caaagatacc agaagatata gattttgcaa tacttgcttt
    20701 acatggaaaa tttggtgaag atggatgtat acaatcaata cttgagacta tggatatacc
    20761 atactctgga tgtggacctc tttgcagtgg gatgtgtatg gataaaaata taactaaaaa
    20821 aatgcttaga gattcaaacc tacctactgc tccatgggtt ttagtaaaat cagttgatga
    20881 aatagactat gatgaaatag ataatatagg ttatccagta tttataaaac caaactcagg
    20941 aggttcaagt gtagccacat tctttattca ttctaaggat gaagttgaag aagcagttag
    21001 aaaaggtctg gaagttgatg aatttgtaat gattgaaaaa tatataccag gtggagaata
    21061 tacttcattc atattaaatg gagaagtgtt ccctacaata tctataaaat ctgattcagg
    21121 attctttgat tatgaagcta aatactcagt tgaaaaaggt gcaaaggaag aagttgttta
    21181 cttagatgaa gaacttcaaa aaagagtaaa cgaaatttct gagacttgtt ggaaaatttt
    21241 taattgcaaa gcatatgtaa gagttgatat gattataagt gaaggtattc catatgttct
    21301 tgaactaaat actcttcctg gaatgactca aactagttta attcctagaa gtgctgctgc
    21361 tagaggtatt aagtacagtg aactattaga taaacttata gaatactctt taaattagat
    21421 gaaataattt gaggtggtaa tttgatattt tcaaatctaa aattaaatga taatgaacca
    21481 atttatatac aactaaaaaa ttatataagt gatatgatat caaaaggact aattccagac
    21541 aatagtaagc tcccttctac aagggagctt agtcagcttt tacaagttag ccgaaattct
    21601 gtagttttaa cttatgaaga attaaaatca gaaggattaa tctactctat ttcaggaaaa
    21661 ggtacatttg ttaaatcaaa gaataagtct tcaaatacta catggtcatt aaactgggat
    21721 tgtttagaga atacatactc taaaaaagcc aatgaattgg acattataaa aagtgaaatt
    21781 ccttggagct ctgacctgat ttcctttaaa agtatttctc cagatggaga tttattcgac
    21841 atggaagaat taaaaaaatc ctttttaaat agaatttcac tagaaggaca taaattactc
    21901 aactatggat atgcacaagg ttataagcca cttattgact atttactaga atatatgaca
    21961 aacaaaggtg ctgatataag taataaagat atactaataa caaatggatt cacagaaggc
    22021 tttgatatta ttatgtcatc ttttacccaa gaaaaagact acataatatg tgaaaatcct
    22081 actcataata ctgctataaa aattatgaaa tctcataacc tcaatatctt aggtgtagat
    22141 ataaatgaag atggtttgga cttcaatatg ctagaagaca atttagttaa gtataaaaat
    22201 aaaataaagt ttgcatacat tacaccctct tatcacaatc ctacaggaat tgtaatgact
    22261 cctgaaaata ggtacaaatt ctataatctt atgaaaaaat acaatatagc aattatagaa
    22321 gatggattta atgaagaatt actctacaat agttctcata tatttcctat ctgttcccta
    22381 gataatatga ataatggggt cgtatatatt ggtagttttt caaaaatttt atttcctgga
    22441 atgagaattg gatggatatt tgcagataaa aaagtaatca ataagctaga aagtgtaaaa
    22501 aggtgcagaa acatacatgt atccttttta gaccaaggaa ttttatttga ttatttaagt
    22561 aatggaggtt ttgaaaaata cattaaaaaa atacgtaaat tctatggaga caaatttaac
    22621 tttgcatata aatgtataaa aaaatatata aaaacagaat atatcttggg tgatggtggt
    22681 ttgcatatat ttataaaact aaaaaatata gatactagag ttttattaga aaaatgttat
    22741 gaaaaaaatg ttatatttat gcctggagac ttatttttta cagataactc tggttttgat
    22801 actttaaggc tcggattttc taggttgtct tttgaagaca ttgaaattgg aataaaaata
    22861 attggagaaa ctataaatga aatgttctag gttaactaaa tattgttagc ctagtttatt
    22921 tttatgaggt ataatatata tagtttatat gaatctaata ctgggggata atatgagtaa
    22981 aaagtcaaaa cgaagaaaaa ttaataaatt aaaattaacg attgtaataa ctgtattatt
    23041 tcttgcttta ttatctatat atgagatact gttctctaat cccaacaaaa aaggtaatat
    23101 aaagagtcaa gaaactatgc aaaataaaaa taatacaaca gatgattcta aaaataatca
    23161 aagctctacc aaaaagcagg aagataataa tatcaaacct actttttcat attcttcaat
    23221 accagataat ataaaaaata aaatgattgg taaatctatg cctactgatg aacctataag
    23281 ctttgatagt ctatcttatt taaaacttac atattatgga tttgatgaaa aaactcatca
    23341 aggagaaatg atagtaaaca gtgaactagc tccagaagta gtggatattt ttaaggaatt
    23401 atacgaaaaa aaatatccaa ttgaaaaaat taagttaata gatgactatg atgctgtaga
    23461 tgaaaaatct atgtctgata ataatacctc atcattttgt tatagaacta tagctggtac
    23521 aaatgtagtt tcaaatcatg gtaaaggacg tgctatagat ataaacccat tacagaatcc
    23581 tcaagtttct ggaaatgatg ttacacctaa agtaagtaca gtttatgctg ataggtctag
    23641 cacaaaattt ggaatgataa aaaaaggtga tgattgttat aatgcttttg taagcagagg
    23701 ttggagttgg ggtggctatt ggaaaaaccc agattatcaa cattttgaaa aataatagat
    23761 tagaaataaa ggtgaattat atgaatttaa tgacattgga aaatataagt aaaagctatt
    23821 ctgaaaaaaa attactagaa aatatatctc ttggaataaa tgaaggggaa aaaataggat
    23881 ttattggtgt aaatggtact ggtaaatcta ccttactcaa aattattgca ggagttgaag
    23941 aaagtgagac tggtaatata acaaaaacta atggtattag gatagaatat ctacctcaaa
    24001 atccaactta caatgaaaat tcaactgtac ttgaacaagt atttaatgga acttctgaag
    24061 aattaaagct acttggtgaa tatcaagaaa tcctagaaaa acttaattca aactttacag
    24121 aggaattaaa taaaaaatta ttatcagtac acgaaaaaat agatgcctta aatctttggg
    24181 atttagaaag tgaagctaag tcagtactta caaagcttgg tataaatgac tttaatcaaa
    24241 aaatagcaga actttctggt gggcaaagaa agagagtctc tctggcatca gcccttatta
    24301 caccttgtga gtttttgata ctagatgagc ctacaaacca tctagacaat gatactatag
    24361 attggcttga ggaatatctt aattccagaa aaggttcatt gcttatgata actcatgata
    24421 gatacttttt agaccgtgtt acaaatagaa ttattgaact ggacaaaggt agattattca
    24481 gttatgatgg gaattattca atatttttgg aaaagaaaat ggaaagactt tcgctagaag
    24541 caagtattga agataaaaga caaaatttaa taagaacaga gcttgcgtgg gtaagacgag
    24601 gagctcaggc tcgtagcact aaacaaaaag ctcgtttaca aagatttgat gaacttataa
    24661 atagagattc ttttaaacct gatgaaaatc tcgatatatc tgttgcatca agtagacttg
    24721 gaaataaaat aatagaaata cacaatatat ctaaatcatt tgaagaaaat actgttatag
    24781 ataatttaga atacacttta actcgtacag atagaattgg tataataggc aaaaatggta
    24841 tgggtaaatc tacccttata aatatactta atggagaaat agagcctgat agtggatata
    24901 tttcaattgg agaaacagtt aaaataggtt gtttttctca agacgactct catatgaata
    24961 ttgatatgag ggctatagac tatgtaaaag aagctagtga ctatatagaa acttcagatg
    25021 gtactaggat tacagcttct caaatgtgtg aaagattcct ttttaatggt actatgcaat
    25081 atactttgat aggtaaatta tctggtggtg aaagaagaag actacatctt cttaaaatcc
    25141 ttatgacagc tcctaatgtg cttcttttag atgaacctac aaatgattta gatattgaaa
    25201 cgcttaaaat attagaagaa tacctagacg aatttaaagg tgtagtagtt acagtatcac
    25261 atgatagata tttcttagat agaatttgta ataaaatatt tgcatatgaa ggtaatggaa
    25321 aaattcatat ctatacaggt aattacagtg attatcttat ttatagagag attcaaggaa
    25381 ttgaatttga agaatctaaa agggaatcag ttaaaaaaga tgactctact accccaaaaa
    25441 aagaaaaacc taaaaatgat aaaaagctta agttctcata taatgaacaa agggagtttg
    25501 aaaatattga ctctgatata gaaaaactcg aatctaaaat tgcaacactt gatgaaagta
    25561 ctgcaaaatt ttcaactgat tttactaaac tccaagaaat tttagatgag aaatctaaac
    25621 tagaaaaaga acttgaatac aaatatgaaa gatgggaata tttaaataat ctagctgatg
    25681 aaatagctaa taataagtaa tattaaaata atcttaagtt taataagttt tgctttaaat
    25741 ctgtaattta taaatatttt aaaggaagtg tctcaaaatg aactttttag ttttatgaga
    25801 cactcttttt tatgagaaat caaatatatt ttcatcattt caacaataca aaagaggctt
    25861 atctctattt tgagacagcc tctttcataa ttaccttcta atttatatgc tctaatatat
    25921 ctataataat tcttattata tctatatatc cagtctttca atatagtgac aattaacttc
    25981 aatatgattt aaaaatacta acaacctttc ataaagtgga tgagattctt ctccatattt
    26041 agcttctagg ttcttgccta tttctctaac agtcctttga ccatcaattt gtaaaaaaac
    26101 aaaactggaa tattcatcta gttctatttt tttgtattta ggtattctag cttttaactt
    26161 tctaaaaaac ttttgaattt tatggtcctg tttttcaagt atagtaacaa cacgattttc
    26221 agctacttca tattcaagtc catcagtaat tttaaataca atatctaaaa catcattact
    26281 cttgctcatt attttttact ttagataaaa caataggtaa tgctgtaagc acaactaaaa
    26341 ctactaataa agcaattgcc attgaatttg ttgcagcaaa tccacttggt acctttggag
    26401 ttacaactcc agttacttgt aatattatac ctattaaacc aatgatagaa ccaccagcaa
    26461 caagaccaga agataaactt ataccattag aaactttaac ttctttttct ttttcagttt
    26521 tagatactaa ttcaacaaat aaacgaatta atgctccaac taatattata gaagtagttg
    26581 caattggtaa atagaatcct atagctatag tcattattgg taaatttaat gcatatagaa
    26641 ctattgccat aaatactcca acaataatca taacccaagg taaactacct gacataatac
    26701 cagaagttaa tgttgacatt aagtttgctt gtggtaatgc aaattgtaca ttatcaccag
    26761 ttgctctaag ttggtcagaa agtaataaaa ttactcctac aacaactata acaccaacta
    26821 tacttgcaat tgtaaagtaa cgttgcattt cgtttttgct accaccaata atataagtta
    26881 ctttttgaga ttgagtataa cctccagcta tagcaatagc aacaactata aatgaaccaa
    26941 ataaaagtaa tgatttatta gcttctaaat cagtccatcc cattataaca aacactaatg
    27001 ttacaataac tagagaagct atagtcatac cagaaactgg aagatttgat gttccgattg
    27061 ttcctgttaa acgaccagct actatgacaa ataataaaga taataaaaga gatatgatag
    27121 ctccaataat tgccataaca atgttaccag aaattaagaa tgctgctaaa aatccaataa
    27181 ctactccacc taataaaagt atcatttgta tagatgaacc ttcttctcct tctccagcac
    27241 ttgatttagc tttaagtgtt tcttttatag aagcgattat agttggtata agttttattg
    27301 caccaataat acctccacaa agcatcatac cagcacctat atattttaca taactactag
    27361 atattgctcc aacatccatt tggttaagag gcattgcagg attattccat accatagctc
    27421 catctttagc catatctgta aagtatccta ttaaaggtgc tataccaaag tttgataaaa
    27481 tacttcctgc aaacattgtt aaagaaactt caagccctac tataaatcca attcctaaaa
    27541 gtagaggatt tacttcaata tcaaatttcc atttgtagaa tttgcttcct acaagactca
    27601 ttacattgtt tgctacattt aagaatgaac cagttaatac agtaataaat ccacttatta
    27661 caaatccaat tcccatgtac ttaattgaat ctccaccagc ctctgaagca acaagtgtct
    27721 cagatatagc cattgattct gggtacataa gtttaccatg ttcttcaact attagatagt
    27781 tgtgaactat agcagcaatt cctataccaa ataaaactcc accaactcca acagctaagc
    27841 cttctaagaa tgtaatttgg cttccaatta atataactgc tggtaatacg aaaataaatc
    27901 cacttgcaac agattctcca ccacttgaca taccttggat taagttttta ccaagaatac
    27961 cctttgaacg agcaaatgct ccaacaaaag cagaacctat tatagctcct ggaatacctg
    28021 cggcaactgt aagaccggct ttcattccag agtaagttgt agatgcggca aaaatggcag
    28081 ctaaaataat accgataatc agtacagcta catttccacc ggttctggac ttatctgtga
    28141 tatatggaac gtaatctttt ccactaacct caccataagc acccttaggt aattttttgt
    28201 tcataaaatt ttcacccctt ttcaatttgt atatattata acatatatac atataaaact
    28261 taacttttta ttattttaca aatagcgagt caaaactact aaaatttaac cctatattat
    28321 taatttctac ttttagatat ttttatgcta attttctttt tatttttcaa ttctactaaa
    28381 aactattaag atataaaata aaaaaatttt ttaatgtaaa attaattttg taaaaaaaat
    28441 gctttaaaac acggaaaagt ataaaaaata caataatcat tcttataaat aaattttcac
    28501 catagtaaat atatacagaa aattcttgtt tttatgcaaa ttgattattt tataaaaata
    28561 agtgtcataa agaaatgtct gaaatcactt tatgacacct atcataataa aaaaattatt
    28621 ctggtaatgc tcttttattt acttttccga ttggtgtaac tggtatctta tctatcctca
    28681 ttattattgt tggaatcata tatggaggta gatgctcatt taaatattct ctaatagttc
    28741 tttcatttat cttaccacta tcttcctcat caattacata atatgcacat atataaatat
    28801 ctccatcatc atttgttctc gatatacaaa caacactttt aatttctgta atattattta
    28861 ttatattttc aatctcatat agctctatcc taaactcatc aatttttact tgattgtcca
    28921 tccttccaat ataatctaaa tttccatcat ctaaccatct tactaaatca ccagttctat
    28981 acattaactt attatgttca caagttttgt aaggattctc tacaaatact tgctctgtta
    29041 gccctttttt atttaaatat cctcttgata tttgaatgcc tgatagacat agctctcctg
    29101 gtgctccaat tggacatagt ttgttctttt tatctaatac atatgcataa gtattaaata
    29161 taggttttcc tattggtatt tttccatatt ctctgtccac atgaaaagaa gtcgatacaa
    29221 cagtaaattc ggttggtcca tactcattat atagttcata attacgcttt ctatatcctt
    29281 ttaatctttc tcctccagtc tgtacaactc taagtgaatt attattgcaa ttttgtataa
    29341 ataaccttgc aaatgctgtt ggcaatgcta gtatagttat gtcattttcc tcacagtatt
    29401 catttactgt atattcatca aatttagctt cttcaggcat aatatctatt gatgcaccta
    29461 caagcaaata agggaatagt tgtttcatta cagcatcaaa actgaaactt aagtatatac
    29521 cacatatatc attttctgta gcgcttatag attttacgct ataataacac atatttacta
    29581 aactcttatg ttctatagct atggtctttg gcctaccagt tgaccctgaa gtagatatcg
    29641 tatatgcaca atttgatgat gttatttttt cagttaattc atatccaaac ttaaacattt
    29701 cttcatcatt tatatcgagt actatggtat tctcatttac aaccttactt ttgagttcat
    29761 ctgtagttat cactactttc gcattactat cttcaagaag gtaattcaat ctttcttctg
    29821 gattacttgt gtctattgga aaaaatgctc cccctgattt cataatccct attgctgtaa
    29881 taattatgtt aatatctctt ggtaacatta cagctactat gtcttcactt tccactccta
    29941 tatgatttaa ataacctgca agtttatcag ttacttcatc caattcttta tatgttaact
    30001 ttctattttc atccctagca gctatattac taggtgttac cttaacttgc tccctaaata
    30061 aatcaacata acttttatca tagggtactt catattgatt attattaaag tcttccaata
    30121 atactttttc ttcttcctta catacaaatt ctaaatcttc tattaaaata tcattatctt
    30181 ctattatttg ctctattata taatcaaatt gatttaaaat tcttctaata gtttctcttt
    30241 tatatataga tggtttatac tttattttta ttttaagtgc cctcttagta tatactatta
    30301 cagataaatc atatcctttt ttttctatgt cataatcata tgataaatca aaacttttag
    30361 ctataaatat tgtgtttata agcttagtat ttagatgatt tttattctct atatctgata
    30421 acaaatagta tccatactct aaagacctgc tccactgcct ttttagcttc cttacaacat
    30481 ctataaattt ttcactttgt ctaactctta ctcttattgg aattatattc attgttttac
    30541 caaaaactac atcatcttca tatatatact tttgaagtat aaggccatat atagtttcta
    30601 tgatagagtc tatgctaata ttgttcactt tagaatattg aattaacctt tcatgtttct
    30661 caatattcat cttaaaaacc tcatctacta cttcatcaga acaatcttta taaaaaaaat
    30721 catttggaat ttttgctata ttcgtataat catctaacaa atccatccaa taattcatag
    30781 ctcgttctaa attaacttca tacatatcaa aattccccct tactactaag tctatagaaa
    30841 aaattctttt ggaattttat tttcacaata tataaatata ttaataatac atatgcaagc
    30901 tctaaaaaca tgagcttgca taaaattaca cttattatga ctactaaatt cgtaatattt
    30961 acaatgttta aaatatatta taacacatat atgattttat ttttctacat ttatataata
    31021 tataaattac aaatagttat ttaactaata tataacaata ccttttggaa tatgatatat
    31081 tatattaatt aaatttttat aagataagtt atacttatag gagggaacca tataaatgaa
    31141 tatttatgct catagggggt ttagtggaaa gtatccagaa aatactattc tagcttttaa
    31201 aaaatgtttg gatatggata tatatggaat agaacttgat gtccacagaa ctaaggatgg
    31261 aaaaattgta gttattcatg atgaaaaggt tgatagaact tttaatggac atggttttgt
    31321 aaaagacttt actttaagaa aattaaaaac tctaaactca tcttttgaag gatatcaaag
    31381 caataaagaa tgtaaaattc caactttaga agaagtatta attctaatct cacctacaga
    31441 ccttacttta aatatcgaac taaaaactga caaaataaac tatcccaata tagaaaaagg
    31501 cgttttagag cttattttaa agtataatat gaaaaataga gttcttatat ctagtttcaa
    31561 cagcaatagc ttaaaaaact ttcataaatt agaccctagt gtaaaaacag gattattgtg
    31621 ttatttacct ataaacaatg tagtcaattt tgctaaattt ctgggtaata gttatcttca
    31681 tccacctcta gttttagtaa atgaatctct aattgaactt tgtcacaaaa atctacttgg
    31741 tgtcaatgta tatactgtaa atgaagagga tgatatacta cactgtctaa aactcaatgt
    31801 agatggtatt tttactaact atccagatat tgcttcaaat cttttacact caaaacaata
    31861 cagctaatta tacaatagtt tgtactcctt gacttttata taattaatat gtacaatttt
    31921 attaaattta tcttggaggt ataactatgg ataacttatt aaatgaactt gaagctttaa
    31981 agagtaattt agataatcta ccaatagaaa aaatagaaaa ttatgatttg agtaaaactg
    32041 ctttatttat aattgatgtt aataatggct ttgcaagaca aggtgcatta tactctccta
    32101 gagtagagtc tttaataaag ccaattgaaa tgtttacaaa aaaaatatca aacaaattaa
    32161 atagagtaat agcatttact gattctcata ctcctaaatc aatagaactt ttgagttatc
    32221 ctgttcactg tttggaaaat gatgtagaaa gtgaattggt tgatgaactt aagtcgatag
    32281 aaaacctaca aattcttcct aaaaattcta ctaatggttt ttttgcatta gaaaatttgg
    32341 attttgataa tatagataat ataattatag ttggcgattg cacagatata tgtatatatc
    32401 aatttgcaat aactctaaaa tcatatttta atcaacataa tattgaaaaa aatatagttg
    32461 tacctatgaa tttagtagat acttatgata ttcctaacgt tcaccctgct gagattttaa
    32521 acttagtatt tttcaacagt atgattcaaa atggagttaa tgtattaaaa gagattcgtt
    32581 aagcttctaa tactttacat cccatataat acaattatca gatatttatt tttaataaaa
    32641 tttctaatag aaaaacacag ttttagtgag aagattacaa tttaaaacac tatctattat
    32701 tactaatctt cacaatataa aaaatcgcag tttagatatt ccttatacaa atttataagg
    32761 aataataaat tgcgattttt atttttaagt agtacaatta attgatacta cttcagtatt
    32821 taaattcatt ttttaataat catacttatt cgtagtttcg cacattacta cctttatttt
    32881 tttattaaac atattcttat attctaataa aaactcattt agttctgaat ccataagatt
    32941 tatttttcta tcactacaaa gcattatctt ttcttggtcg attattacat taatatcatt
    33001 taagtcattt tttataaatg ttgttataca actctttggt tggatttttt ctccatattt
    33061 ctcataaaat cctgcactct tcttaaatga ctctaaatct tctgtagata ctttgctgga
    33121 agtattatta ctcataaaac ctgccgtaga agggtcgatt ccaaagtttc taagttgttt
    33181 ttctacttct tcagtgtcca taccatatct ctccataaat tgcttttgta ttttcataaa
    33241 tttttcttga gatatgttgt tatctctcat ataatcgaat atttttcttg aaaactcagc
    33301 catagacatc tttccatcca taacagaaaa atataagtta tatatgtatt gctcaaattt
    33361 atctacatca gaatctttaa ctttgttctt tagttcgtta aagtctatta ttctatcatc
    33421 agacattttt catcctccta gctaaatttt tacttgtata agattatacc atatttctat
    33481 tatatttata tacacactat ttttttaatt taacgatttt ttctgtagca tctaattcgc
    33541 acattctgcc caaaggaata gttatcattg ggatacaatg tcctgactga agattatata
    33601 tagaaggttt attgacctta tttgcaaatt ctattagtaa ttccatcaaa tcattttctt
    33661 caatacaatc cttaaaatct ccaaaaatta taccattaca ttcctcaagt tttcctgaag
    33721 aatatagttg tgttaacatt ctatccagtt tatattgagg ttctccaatt tcctcaataa
    33781 acaaaatttt atcttttgca tctatttcat attttgttcc cattgtggca attattaagg
    33841 ataaatttcc tcccattatt attccctctg ctttaccttc acatacagta taaatctttt
    33901 cttcttttgg attttctaaa tataattcat ctttaaattc taaagcattt accaatgatt
    33961 tataggtaaa tttatcccat ttacctacat ttgctgccat tattccatga tatggtaaaa
    34021 gaccacaatt tttatagaaa gctaaattta aacctgttat atcagaaaaa cctataaatg
    34081 gttttggatt ttgctttatt atatcataat ttattttatc caatattctt gttgttccat
    34141 aacctcctct taagcacata attatatcta catctttatc taaaaacata tcttctatat
    34201 cccttgctct aatatcatca ttaccagcta aataaccttt ataatttaaa ctacaacttt
    34261 cagaaaactt aacttcataa ccataacttt ctaaattaaa ctttatttcc tccaaacttg
    34321 tatttcttaa tggcccagat ggcgcaatta ttcctaacat ttaaacacct cttcttataa
    34381 aatgatatat tcttctccat actaaatatc tctaatataa ttataagata ttttatttgt
    34441 acttgataca taataatgat ttacataaaa taatataatt aatgaagaaa aataaatttt
    34501 taatatagtt aggatgtgtt ttatgcttaa tataaaaact ctttgtcatg agataaatga
    34561 ttgggttata aatataagaa gagatttaca taagactcca gaacttggat tagaagaatt
    34621 tcaaactaag aaaaaaatta ttaaatactt aaatgaaata ggtataaatt atattgaata
    34681 taagaatcat acagggataa ctgcatacat taacgtgagt ccaaactttg aaacagttgc
    34741 tataagagct gatatagatg ccttacctat aacagaagaa cttaattatt cttataaatc
    34801 tattaatatt ggcaaaatgc atgcatgtgg tcatgatgca cacactgcta ttttactggg
    34861 tacttgtaat atactattta aattaaagga ttacttaaat gtaaatgtaa aattcttttt
    34921 tcaacctgcc gaggaaacta ttggtggggc tcaacttatg atagaagatg gatgtcttga
    34981 aaacccaaat gtaaaatata tatttggact tcatgtaaat tccaatatca acaaaaatct
    35041 aattgaatta aaatacaaca ctctaaatgc ttccactgat acacttcaat taactgtaca
    35101 tggttcaaaa tgtcatggag cttatccaca tcaaggtatt gatgctattg ttatatctgc
    35161 tcatattatt acagctctac agacaatcgt tagtcgaaac acaaatccaa ctgattctgt
    35221 ggttatttct ctaggaaaga ttgaaggtgg aatcaaggaa aatatagttt gtgataaagt
    35281 ggttataagg ggtactttga gaaccttgac tcctgaaact agagagtttt caaaaaaacg
    35341 aattagagaa atttgcgatt ttacttgtaa aacatttgga ggaagtattt ctgttgaaat
    35401 tgaggaaggt tatccagctc ttatcaattc aaaccatcta gtagattacg tcaaacaaaa
    35461 tgctgtagaa ttgtttggag aagaaaatat tatccttaaa gactcgccca ctcttggggc
    35521 tgaagacttt tcgtattttc taagacactg tgaaggagct ttttaccacc ttggttgtgc
    35581 aaatcgtgaa aaaaacatta cttccccttt gcatacctct acttttgata ttgacgaaga
    35641 ttgccttatt actggtgtca tgctccatgt aaaaaatgta ttatcattct aactattaat
    35701 atttttattt attattttat attgaccctc acatgatgtc atgttatacc ctaaaattac
    35761 aaatgataaa ttaaataatt ggaggtaata ttatgaaaga aatattaata aatcctatag
    35821 gtaaaatatg tattcaaggc gaagaggtct ttattaaatt agacaaaaag tatgtacctg
    35881 cattaaatga actcgacgat ttcagtcatt taaatgtatt ttggtgggct gatgggtttg
    35941 ataatcctga actaagaagt attttagaaa ccccaaaacc ttataaaagt ggaccagacg
    36001 ttataggaat atttgcaaca aggtcaccta taagacccaa tccaatagca ttgactgctg
    36061 tacaaattat caatatcgac catgaaaatg gtataataaa aataccatat attgatgcta
    36121 atgataatag tcctgttata gacttaaagc catatactcc aagcgttgat agggttgaaa
    36181 atccgtccat tcctaaatgg tgtagtcact ggcctaagag tgtagaaaaa tcaggagaat
    36241 ttaattggga agatgaaatt gaattttaaa ttactttttt cttgatttca ttaacttgtt
    36301 ttccacattt attttattta cacttaccaa gattttatgt cacatataca tataatatta
    36361 ggctgtatta tataatacag cctaatatta tataataaat ttctattttt cccttaaatc
    36421 attcacattc tttctaagct acataagatt tttctattat ttctttttga gtttaattta
    36481 gcattatgtt gaggaaaaaa catttcatgc attttaataa attaaatctt tcttttattc
    36541 tgcgatttta taataatcaa tttaacttaa aaataatacc taactatttt aactatgtta
    36601 aactagtaaa tgtaaccact tatatgttac aattttatat aaacatcaga tataaaaact
    36661 atataaataa atgacacata gtgttatatt ttttaatacc aatttagtta tttttcagtt
    36721 acagatttaa taataattaa tagtaagaca taatattttt atgcccttta ttgattatgt
    36781 attttctgtt atctgttatt atcatcaata ataaacataa gggggtatat attatgaaat
    36841 tgtatttaag tgctgatata gaaggtactt gtggtattgt aaattgggat gaaacaaaac
    36901 ttgaatgtga attttcgcaa cactatagaa cacaaatgag taaagagata acagcagcat
    36961 gtgatggagc tactcaatct ggtgtacatg aaatacttgt taaagatgca catgacagtg
    37021 gtagaaatat taatccaaat attttacctg aaaacgtcaa aatacttaga ggatggacca
    37081 aagacccact agttatgatg gctggaattg atgaaagctt tgatgcatgt atattcaccg
    37141 gctatcactc tggagctact tgtaatggta atccactttc ccatactatg gatattgatt
    37201 atgactattt taaaataaat gatgaaatag catctgaatt tactataaat gcttatattg
    37261 ctgcatatta taatgttcct gtagcatttt taagtggaga tgaaatgctt tgtgagagtg
    37321 caaaaaaact aaatccaaat atagtcactg ttcctgtgtc aaaaggaatt ggaaatggtt
    37381 caatttcaat ccatccaaac cttgcactaa aaaaaattga aaaaggtgtc aaaaaagctt
    37441 tatctggaga tttatctagg cacttaataa aattgccaga taaatttaag atagaaataa
    37501 aatttagaga acattataaa gcttttaagg cctcttttta tccaaatatg aaaaaaatag
    37561 attctcaaac agtactattt gaaacagagg actattatga atttttaaga atgttattat
    37621 ttatataaat ttcatatcat aaattaagga gttgattaag ataagtaata aaagaaggat
    37681 tcctttaggc gaatttattt ctgtattttt attttttgct gttttttttg gtataacttt
    37741 ttgtattttc acggttatag gaattggctt cttatcattc ttaggatttg aatataaatc
    37801 tcttggagct gtattaattt tctttttaat atatttttgt ataaccactc caattgattt
    37861 tctatgcact actattttag atatattcag atatgtaaac aaattaccat actcaatata
    37921 taaactttgc gaatttataa ttgattttat actaactttt ctagctatga acataattga
    37981 tacctttatg gattcagtaa cgattccttt aagtacagaa atcttattcg ctttactttc
    38041 ttatctttta tcagaatgta tggatttttt tgatagggat aagaaaaaac cttaataaaa
    38101 aagatagatt ccttaataga acacatattt tcgtatttct attatttaat ctatctttat
    38161 tttatttaat aaatttttat taattaaaat atagtttctt ctatctcctt agtcatttgc
    38221 ttagcactat caaaaaattc ttctatatac actacttcat cactgaccct agaatattct
    38281 gctgcaaatg ccattacatg actttgtata gcttcttttg ctgctactct acccttttct
    38341 agttctttat ttgaaactaa tttaataaat tcagctataa gatttctatc atattcctct
    38401 accatattct gttgatttga tggattcata actatttctt cgttttttcc aaattcttta
    38461 attcttatct catttttatt taaatcccca ccaacttcac catggctaaa cattaacttt
    38521 atagttcttc cattttcttt agtaaatgca cataaattaa aagtagccgt cactccattt
    38581 tcaaactgta gtatatttac catattgtct acaacattat tatcacatct gtaaacacat
    38641 cttccatatg gacttgtctt aagtatattt aatagattct cttcagttgg atttatgtga
    38701 acagctctat ttatacttct atctttttct aaataaattc tctttgctga atatggacat
    38761 tttttttcaa ctaaacactg aagacaattt tgtgccatct ctccactagc attttggtta
    38821 ttaagatgct ttaagcttcc aaatgcagat atttttttgc atctacttcc tactaaatat
    38881 aacaaaatat ccatgtcatg acaactcttt gctaaaatta aagggctggt ttcattgcta
    38941 tttctccaat tccctctagt aaagctatgt gcaaaatgcc aatatcctat attttcatta
    39001 tattgtatac taactagctc tcctaatttt ttgctttcta caatttcttt taatttatta
    39061 taaaatggag aatatctcag tacatggcaa atcataaaaa tcttatcttt atacttatca
    39121 cacaagtcat ctatatgtac cagaccatct aaactattag acattggctt ttctaatagt
    39181 acatgataac ctttttcaag agctagtttt gctacatcat aatgtctatc atcatttgta
    39241 gctattatta ctgcatctgc catcttgtca tgtttaaaaa agtcttctaa agtttcaaat
    39301 acatttttac tatctaggtt atatttttgt gtaaatagtt ctcgtcttcc ttttttaggt
    39361 tctgctacag caactatttc acatacatca gaattttcat aaatatatgg agcatatgca
    39421 tacgttcctc tattacctgc tccaatcaca gcaactttca ctttttccat actttaattt
    39481 atccccttta tattatttat actactctat tatagtctat ttttacagac atttggatat
    39541 ttttaataaa agaaacagca gttataattt ttcaatttta actgctgtta taagttaata
    39601 taccaaacat ttttaattaa attttcttta attttaatac aaagacttaa atattcaata
    39661 caaattataa aaacactttt atctagttta cttcttcttt tctttgtcta atttatctaa
    39721 cttcttattg tataaaaaca tataaaatgc tgccgaaaat actattaaat atccaacaac
    39781 acttaacata tctggataga cattgaataa gaatatactt aaaattgctg aaaagattat
    39841 attactatag tcaaatatag agatttcctt agcaggtgca tatttatatg ctagagttat
    39901 tccaaattga cctagacttg caaatactcc ccctagtaat agatatataa gttgcataag
    39961 ggtcattggt tcatatactg caaaagcaat tggccctaca gtaactaatg aaaaaactga
    40021 aaagaaaaat acaattgtat agtaatcttc tctatctcca agaactctta aacaagtata
    40081 tgccattgct gcaaatacag ctcctgcaat tcccccaaga tatggaatta tttctacact
    40141 aaatgttggt tttattatga ataaagctcc tataaatgca attataatag ctcctatctg
    40201 ctttacattt attttttctt ttagagcaat tgcacaaaat attactacaa ggaagggact
    40261 cattttattt agcatattag catctgaaag catcaacttg tcaattgcat agtagttaaa
    40321 tataactcct agagttccaa atattgacct atataaaagt acttttaaat tttctttttt
    40381 accagttaga cttcctttat gttttatgat aagccaaaaa gctattatag ctgataccag
    40441 atttctaaag aacacttttt gaaaacttgg taaatctcca gctagcttta cgaatgcaga
    40501 catcattgca aagccaagcg ctgatgctat tataaacatt atacctttta ttcgatttga
    40561 tttgattgtg ctactcatac gtacctcttt tctatataaa tgtataaaac catattaatt
    40621 aaaaacttag cttatacatt aaataataat tatctaaata ttccaattta tactataatt
    40681 aaaagtgtaa actatgtcaa gtaactaata aatcttttca attaataaca ataataatta
    40741 tagctaaaaa gttatatgtt tttatggatt attatcagaa tatataatat aattttatat
    40801 agtatagact ttttttaatt tattatttag atttttaata gattttttaa ctatggaggt
    40861 atttaaaata tggaaataaa gagatatgaa ggaactggaa gaatgagtag agctgtagtt
    40921 cataacaata cagtttattt atgtggacaa actcatgctg aaggaggagt aattgaacaa
    40981 acaactgaag ttttagctaa aatagaagat ttattaaaca aatatggttc agataagcaa
    41041 catttgctat ctgttactat ttatttaaga gatatgaaag actttgaagc tatgaactct
    41101 gtttgggatg cttgggttga aaaaggattt gaacctgcta gagcttgtgt tgaagctaga
    41161 cttgctagag agcatttgtt agttgaaatg agcgttgttg cagctacaaa ataatcaatt
    41221 tagcttatag gaggctatct caaaacagag agataagcct ctttttcatt gttgaaatga
    41281 tgaaaataca tttgattttg tataaaagaa agtgtctcac gaactaaaaa attcattttg
    41341 agacacctcc tcactattta atctatataa atttacttat tctctattgg caatatcaat
    41401 ttagatggat atttacctcc atgataaatc gtattctttg cttctatata ttcaatacta
    41461 ttatatccct ctgtcgtgtt gctattttgg aaaataaagt ttttagcact agacgttata
    41521 tctagtctaa ttttatgacc aactttaaac gtgtttgata tttttgaagt tataatcgta
    41581 aacttataaa tctctccttc ttccataaac tctgacttat aaaaactatt tctaaatcta
    41641 gcactaagta taccatcagc taatttaatt gaatttccat ctaaatccac gtccataatt
    41701 tttataaccc agtcagtatc tttggcagaa cttgatgcaa aaaactctaa ctttatatca
    41761 cctgttacag taaattcttc actaaaagca tctgatgtat aacacaacat atcacttctt
    41821 ttttctaaat ctttgtagtt atttggaact ccaacttcat tctctgacat atcaattagt
    41881 tgtactgatg ggtcttttgg attatatata taactatcat acttttcttc taaaccatta
    41941 tcaaatacta atctaccatt tcctaaagat gtgttagcat ttccatcact tattagatac
    42001 atacttttat ctatcttatt tattattggc caattttctt ctgtcttcca cttattagaa
    42061 cctacagagt aatactctac tggagcagtc gtatctatgt tattttctat accttttaag
    42121 tatttatcaa accataatag ataattataa tccaaatcat atcttaatga actatttcct
    42181 aaagaaatac catttatatc tcttaaagta tttgaattat gcatccatgg acctagaata
    42241 gcctttctct ttcctttttc atagtccttt ataacatcta aggcctctgt agttcccata
    42301 ctattatcat cataccaccc agatacaacc aatgcaggta catttatttt atctttttgt
    42361 aaatgccaat ccatcaaatc ccaatactca tctttttcta ctctctttaa ccattcttcc
    42421 agaaattcaa ttctatatcc aagagcttca acaggtatat tttctattgg tcgtatgttt
    42481 aaaacatcat cccaatcatc tcttaccatt ttgcttctat caactttatt tcttgatacc
    42541 ataaatgccc atgcaagcat accagataca aaagctcctc cttttcttgg tatatcaata
    42601 aaagggcttc ctgatgttac tatactaact aaagctttaa gatgcttatt gcctgaagat
    42661 gcagctgccc attggacata accaagatat gatgctccaa gcattccaac tattccactt
    42721 gaccattcct gtgatactat ccaatttatg gtactatctc catcttccct ctcatgaatc
    42781 ataggttccc acttaccttc tgattcattt cttcccctaa catcttgtat tacaacagca
    42841 tatcctcttt gtacatattt ataatatatt tctttatcat tttcttttcc atatggagtt
    42901 ctcataagaa ttgtcggtgc ttttttggta gaatctacaa aatcagggag atatacatct
    42961 gtagaaagtt ttataccatc tttcatatca atcatataat ttccaccaaa tcttatagaa
    43021 tatatctctt catttttatg agttttttcc cattctttta atacagtata ctcttcatat
    43081 ccatccataa ttaatatagt acaagtttct ctggataaac agataaaacc tactatttta
    43141 ttatctacaa taattatatc tagtgggaac tttttgtttc tttgaaccca tatatattca
    43201 ttaagtttag aatgcgtttc tcctaagtta tttataactg tgtctttatc tgtgtttact
    43261 aagcttttag tatatacatc aaaattatta aataaagact ttaaatctat ttttgtaaga
    43321 tttaaatagt atgtattttc ttccttgctt attggtttta aactttctat tttatttgta
    43381 aaaggattca acttttcaat taataaatct ccatcaaaga ttacatttcc actgtatagt
    43441 ccagatatgt ataatttaaa ttttatcaat taaactcccc cctataatcc tagagacatc
    43501 tttattgcat atgccactga tatacctgca taatttccta atactccacc aattactccc
    43561 attacgactg ctacacttac aagcgaattc cactttgcag aagcagctac taaagctgaa
    43621 gtaggaccat cagcaattgc tcctactata gaaagtatta catattgata ctttatcttt
    43681 aaaagtctag ttatgcctaa atggaaaaca aatgaagcaa aaattatgct aaaacagtaa
    43741 agtgttatag taaaagttga acctaaaaat tcttttatat ctactgaaaa acctattgta
    43801 cataaaaaga ataatgcaac aaataatcct aaatctaaat ttccctttag tttctttact
    43861 ggctttaatt gagcaattat aattgaaatt gttgtaacta aaagtattct tgctgcacta
    43921 ctaaaactcg aattgaaata tccagcaagt attgttgcaa cttctgtaac aacaaaacct
    43981 atagccaaca tccaagctat atctttaata ctccattctt ttgactccat aaattcttca
    44041 ttttgacaat cctctaattc tgattcttct acatgatatg gccaaagttt tttaaacttc
    44101 tttgaatttt ttaatattgc tggtagagca aacataaata tcaatgttgg tactccaatt
    44161 acatagtctg ctgcatttgc tgcagcaaga gtttgtctac ttacattaag acctgttcca
    44221 atagctgtaa gattagcact tccaccagta tatgtaccta caaacattcc agcaaccttc
    44281 caaccttctg atattttttc tgcaaatact aatccaaata aaaatgccat gatacttaca
    44341 gttaatacag ctacaaaaat tgcaattaaa ggttctcttg ataattttgt catttctttt
    44401 aagtcaacac tcatcaacat tatagtcata gaaactggaa tagcatatgt tgaaatagtt
    44461 ccatataact cagtacttac tggaactact tttaagtttg aaagtattat tcccattatg
    44521 attattgtaa gtgctggtcc taagctttta atggctttgt atctttgtaa gtaaaaaccc
    44581 aatgcaatca ttgatgtcat tacagcaagt atactacctg gatttgaaaa aagtgtattc
    44641 atatttaacc tccaaattta tcataagttt acttctattc ctaaacctgg tttattgaca
    44701 agattcataa tatctccatc cattacaaat cctccagata taccttctaa ttcttcacaa
    44761 aacataaaac tatcaaggtc agcttctgtt atgttctttt tagatgctat taaatttgct
    44821 gctgctgtta taccaattct cgtttccagc atacatccta acatacaatt tactccactt
    44881 gcctcagcta tgttgttgat ttttatagca ggatatattc cacttgattt catcagctta
    44941 atattaaatg tatcaactgc attctttttt atagccttaa ttgcatctat tggtgaatgt
    45001 aatgattcat ctatcataat ttttgtatta cttttatttc ttatgtaagc agtaccatct
    45061 aaatcccaat gtggaagtgc ttgttcaatt gcatcaacat taaattcttc taactttttg
    45121 atagtattta tactgctatt tacgttccat ccttgatttg catcaattct aattcttata
    45181 ctctctccaa cggcttctct aattaacttt acagcttcaa tatcgtcttc aggatttata
    45241 cctgctttaa gcttcaatat cctaaaacct tcttttactc tttctaaagc ttctactgcc
    45301 attttttctg gcttatctat acttatagta atatccgttt ggactttact atcaaatcct
    45361 ccaagaactt tataaagggg tgcattcata atttttcctt taatatcata taaagctatg
    45421 tcaatagctg cttttgcaga agtattacct ataataattc catccatgat tgtatgtatg
    45481 gtttcaatat ctagtgggtc tttgcctatt aactcttttt taaacataag caatactgaa
    45541 agaacattat ccaaaacttc acctgtaact gctgcaagag gcgctgcttc gccaaatcca
    45601 caataccctt catcagtttc aatttttaaa attatacttt ctccatattc aattactcca
    45661 aatgaaacta ctacaggttt ttttaattta attctaagct tttcaaattt gatatctgtt
    45721 atcttcatat taccctcctt attttttgaa gttataattc aatttttaaa attttatact
    45781 ttaaattata ttattacttc aatatatatg tcaagataat ttactgaaaa tttactcttt
    45841 tccaaaatat aatgtgataa tcttaattca attaatactc ttctattaaa tttttttatt
    45901 attattttct ttgtattaat ttaacaataa ttcattttca tagactttta tttggattta
    45961 attaaatttt aattgtcaaa atctactatg tattcttatt gccactactt ctatgttgtg
    46021 ctataattac tttgttttaa gcgaatattt tatattaatt ttattatttg gaggttctta
    46081 tgctaggtga aaagatgaga aatataagaa aaagcaagaa aaaaacatta agtgatgttt
    46141 ccaaattgac agatttatct ataagttata tctctcaaat agaaagagat gctattgaac
    46201 catccttgtc ttcattgaga aaaatcgctg aagttttaga cactccttta tatatgttta
    46261 tggatgacaa taatactgat gatttagtca ttagaaaaga agatagagtt atgatgaaat
    46321 ttccaaagag tgaaatgttt tacgagatag tatctccaat gcctacaact gagtttacac
    46381 cttcaatttt atttgtagag tttgaactta aaccagaaag tgaggatgcc aaagattata
    46441 tatatcatgc atcagaagaa attatggtgg taacgagtgg tgtggttgat atttgtatgg
    46501 gtgaaaaggt agttagatta aatccaggtg actcaacatt tataaaagct aatgtaccac
    46561 ataaaatcat caatccaagt aaagatacaa tagctcgtgg atatggagtt atatcacctc
    46621 caatttggcc aataaaatct aaataaactc aatatgacac acctactttt tgtagataat
    46681 ataatagatt taaatattgc ttttatttta aaattgaagt atttatttaa atttaaaata
    46741 tttactcaaa ctaaaaaaat agctatttga ctaatctaaa ccatcttaat tatggtaaat
    46801 agtaaatagc tatttgtaag ttatattata aactataata aaaattgtat ttgttaaaat
    46861 taaactgcta tatcatttaa tacttttgcc tttacaactc ttatattttt catactcttg
    46921 tagatatctt gaatcttatc ttcttgctct ctcaatagtc tatgtgcttc atatatatct
    46981 acaagttcaa attttactac atcatttggc tttgcttgtg ctaacttata caaatctaca
    47041 cttataacgt tccctatctt agtatagcca ccactagtct gtctatctga taacattata
    47101 ataggttttc catgaccagg tacttgtata gctccataag aaataccctc agatataata
    47161 tctgctccat taaggtgctt tatactctct ccatctagtc tacaacccat tctatctatt
    47221 tcattcgtaa ctttgtattt atttgataaa aatttctcta cttcaccgtt agaaaaagca
    47281 tcaaattgag gaccttttac aattcttagt acgatttctg aactataagt tggaatatat
    47341 tttgaactta attttttatt tattgtaaaa tctttttcct gaataaaagt atttatataa
    47401 tcgccttttt taagtgctct accttcaaat ccaccaattt tagctcttgt ataagttgac
    47461 ttacttccca ttgcaagtgg aacatctatt ccaccagcta ttgacacata ggctctagta
    47521 cctgatttca cacctctaaa tgataataca tcacctggat ttatttgata tgacctccag
    47581 ttttgaattt cttttccatt tattttagcc cctaaatctc ccccagttac tgctatttgc
    47641 gtatattcat caaaggtaat tgtaggacct aacattaaaa cttctaaaga agcctcatct
    47701 tcagaatttc ctactaatat atttcctact ctatgtgcat actcatccat tgctccacat
    47761 ccagatacac catattgacc atagccaact ctacctctgt cttgtattaa tgtttggaaa
    47821 cctcctaatt caactttgaa tcccatatat accactccct gtactataac gatatttaaa
    47881 tacttaagtt atttttttcg atttctataa attcatctaa tgttattgga acaaatttta
    47941 taaagtctcc agctctaagc aaaatctgtt cttctctatt ttcatcatat aattttaaag
    48001 gtgtcctacc aattatttgc catccaccag gactatcaat aggatataca ccagtctggg
    48061 aacctgctat tccaacactc ccaccataaa ttttagttct tggcacttct aatctaggtg
    48121 tagctattct ttcatccata ccacctagat atggaaatcc aggagtaaac cctagcatat
    48181 atatcaagta ctctctgctt gtgtgtattt ttataacttc atctacagtg agattattgt
    48241 agcttgcaac gtttttaata tctataccaa aaacctcatc ataacatact ggaatctcat
    48301 gtattttagg ttttggtaat actatacttt ccaaattatc ttctattttt tttaaattct
    48361 caatcaaact atcaaaatct atttctaatg gattataact tatcataagt gacctatatg
    48421 ttggtaccat ttctgttaca atacctttaa gattcgatat ttctattgct ctcataaatg
    48481 aaattacttt tttgtttata tcttctgata tctcatttcc aaattcagca accactgcct
    48541 tatcccctga tatcaagtat ctagtttcca taaatgtcac ctcttctaaa ttaagcaaat
    48601 aaagcaccta ggctgctaag agaagttatt cctgtatatg cagatattaa aactactatt
    48661 aatccaaata ttaaaagcca tgtaggatgc ttgtaatctc caacaatatc ttttctttta
    48721 gatgctataa gcataattcc taatgtgatt ggaagtatca atccatttaa agctcctgct
    48781 aaaattaata aagtagctgg ttttcctata aatgccatta taagtgttga tattgctata
    48841 aatgctatta taaaatattt ttcatattta tctataaaag gatttagtgt ctttaagaaa
    48901 gaaactgatg tataagcagc tcctataact gatgtaattg cagcagacca taatacaagt
    48961 ccaaagaatt tatatcctat tgctcctgca ctaaatttaa aagctgaagc tgctggattt
    49021 tctgggtcaa gttgaagacc ttttgaaacc actgccagta ttgctaaaaa taaaagaact
    49081 ctcatcattg tggcaactaa tatacccaaa agagaagatt tagttatttc ttttatattt
    49141 tcttctcctg ttattccacc gtctattaat ctatgtcctc cagcaaaagt tatataacct
    49201 ccaacacttc ctccaagtaa agttattatt gggaatatca gtccttttgg attttctggt
    49261 tttaccattc ttgatactgc ctctccaaca ggaggatgat ttgatattgc tacatatcca
    49321 actataatta tcattcctaa tgctaaaaat ttagttatct tatcgactag agaatttgaa
    49381 ttttttgaca taaatacaaa tatagctatt aatccactta atacagttcc taatgtcata
    49441 ttcatattaa acattacatt cataccaagt gcagctccac caacatttcc tatattaaaa
    49501 gctaatcctc caagagctac taaaaaagct actaaatagc ctaatccagg tattatttta
    49561 tttgctatat cctgacctct aagtcctgat acaccaatta ctctccatac atttacttga
    49621 gcccctataa aaaggattgt tgtaattaaa attacaaatg aaaaattcgg tccaaaatct
    49681 tgggtaaatt gtgcagtctg agttaaaaac cctggtccaa ttgctgatgt tgccattata
    49741 aatgcagccc ctataagtgc tccgacatct tttttatttt tagttttatc tgtaatattt
    49801 tttgtactca tattttatct ccccctcatg ttatcagtct tatactatgt tctctgacga
    49861 acaaacttct atgttttcta atggacaaac ttctatacct tctaattcaa aacgctttct
    49921 tatttctttg acaaactcta ttgcttttgg attgtcccca tgtacacata tagaatctgc
    49981 tttgatatgt ataacttcac ctgttaaagt ttctacagtt ccttctttta ccatcctaac
    50041 aaccctatct attgcatgtt ttgtatcatg tataactgct ccttctacat ttcttggaac
    50101 caaaaaacca ttattatcat aagctctatc tgcaaatact tcatttgcaa acctaagtcc
    50161 aatttcttta gcagaattta tcatttcact attatatagc cccaacagta taatatcttt
    50221 atcgacttca tatatagctt cacaaatccc catagctaac tctttatctt tagctgccat
    50281 attgtataat gctccatgtg gttttacatg ttgtatatta catccatttg aacttgcaaa
    50341 tgccattaaa gcacccagtt gatattttgt ataatcttta gcttcctgct ttgttatttt
    50401 catctcacgt cttccaaatc ctattaaatc taggaaacct ggatgtgcgc ctattttcac
    50461 tccatttttt ttagctagct gtactgtttt ttccatatga ctagggtcac ctgcatgaaa
    50521 cccacaagct atattggctg atgaaatata ttttaaaact tcttcatcta aaccaatctt
    50581 atacgttcca aaactttctc ctaaatcact atttaaatct actttataca atttatgacc
    50641 cctcctcgct aacattttga tagaataagt agttttaatt tttaaatttt gtttatttta
    50701 tatattgcat gaaatatttt atcacttgta ttcaatttta tttttgcaac tattctattg
    50761 catttaatta atataatttt accatttaag gaatattttt gcaattattt taattgaaca
    50821 ttcatttgta tttctttttc aaaaaatctt tttaccattt tttagttaat aattaattat
    50881 tttcacttaa aataagggct ttaaccttca tttttaaagt atttttcatt tttaatcact
    50941 ttatatgcaa gacagaatca tttttcttca ttcatttgtt tctttcatct atataattat
    51001 cattctttgt aagtaaaata tctttatatt
//
LOCUS       DAMJYX010000018        49510 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJYX010000018 DAMJYX010000000
VERSION     DAMJYX010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.018"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(140..3475)
                     /locus_tag="PC479_001148"
     CDS             complement(140..3475)
                     /locus_tag="PC479_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2803702.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3525..3953)
                     /locus_tag="PC479_001149"
     CDS             complement(3525..3953)
                     /locus_tag="PC479_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803703.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3943..4323)
                     /locus_tag="PC479_001150"
     CDS             complement(3943..4323)
                     /locus_tag="PC479_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2803704.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4356..5528)
                     /locus_tag="PC479_001151"
     CDS             complement(4356..5528)
                     /locus_tag="PC479_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2803705.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(5532..6995)
                     /locus_tag="PC479_001152"
     CDS             complement(5532..6995)
                     /locus_tag="PC479_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2803706.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(7505..8374)
                     /gene="gatY"
                     /locus_tag="PC479_001153"
     CDS             complement(7505..8374)
                     /gene="gatY"
                     /locus_tag="PC479_001153"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2803707.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(8394..10310)
                     /locus_tag="PC479_001154"
     CDS             complement(8394..10310)
                     /locus_tag="PC479_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2803708.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(10313..11233)
                     /locus_tag="PC479_001155"
     CDS             complement(10313..11233)
                     /locus_tag="PC479_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2803709.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11335..12087)
                     /locus_tag="PC479_001156"
     CDS             complement(11335..12087)
                     /locus_tag="PC479_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2803710.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(12479..13924)
                     /locus_tag="PC479_001157"
     CDS             complement(12479..13924)
                     /locus_tag="PC479_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2803711.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(13946..14299)
                     /locus_tag="PC479_001158"
     CDS             complement(13946..14299)
                     /locus_tag="PC479_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2803712.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(14328..15575)
                     /locus_tag="PC479_001159"
     CDS             complement(14328..15575)
                     /locus_tag="PC479_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2803713.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(15583..15900)
                     /locus_tag="PC479_001160"
     CDS             complement(15583..15900)
                     /locus_tag="PC479_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803714.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            16142..18055
                     /locus_tag="PC479_001161"
     CDS             16142..18055
                     /locus_tag="PC479_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2803715.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(18178..19332)
                     /locus_tag="PC479_001162"
     CDS             complement(18178..19332)
                     /locus_tag="PC479_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2803716.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(19611..22226)
                     /gene="mngB"
                     /locus_tag="PC479_001163"
     CDS             complement(19611..22226)
                     /gene="mngB"
                     /locus_tag="PC479_001163"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2803717.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(22370..24295)
                     /gene="mngA"
                     /locus_tag="PC479_001164"
     CDS             complement(22370..24295)
                     /gene="mngA"
                     /locus_tag="PC479_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2803718.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(24425..25150)
                     /locus_tag="PC479_001165"
     CDS             complement(24425..25150)
                     /locus_tag="PC479_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2803719.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(25413..26192)
                     /locus_tag="PC479_001166"
     CDS             complement(25413..26192)
                     /locus_tag="PC479_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2803720.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(26185..27612)
                     /locus_tag="PC479_001167"
     CDS             complement(26185..27612)
                     /locus_tag="PC479_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2803721.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            28003..28725
                     /locus_tag="PC479_001168"
     CDS             28003..28725
                     /locus_tag="PC479_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2803722.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            28922..30589
                     /locus_tag="PC479_001169"
     CDS             28922..30589
                     /locus_tag="PC479_001169"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2803723.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(30685..32004)
                     /locus_tag="PC479_001170"
     CDS             complement(30685..32004)
                     /locus_tag="PC479_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2803724.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(32045..32791)
                     /locus_tag="PC479_001171"
     CDS             complement(32045..32791)
                     /locus_tag="PC479_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2803725.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(33035..35044)
                     /locus_tag="PC479_001172"
     CDS             complement(33035..35044)
                     /locus_tag="PC479_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803726.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(35482..36915)
                     /locus_tag="PC479_001173"
     CDS             complement(35482..36915)
                     /locus_tag="PC479_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2803727.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(37002..38918)
                     /locus_tag="PC479_001174"
     CDS             complement(37002..38918)
                     /locus_tag="PC479_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2803728.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(38992..39852)
                     /locus_tag="PC479_001175"
     CDS             complement(38992..39852)
                     /locus_tag="PC479_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2803729.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(40203..41390)
                     /locus_tag="PC479_001176"
     CDS             complement(40203..41390)
                     /locus_tag="PC479_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2803730.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(41404..42795)
                     /locus_tag="PC479_001177"
     CDS             complement(41404..42795)
                     /locus_tag="PC479_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2803731.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(43390..45090)
                     /locus_tag="PC479_001178"
     CDS             complement(43390..45090)
                     /locus_tag="PC479_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2803732.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(45062..46465)
                     /locus_tag="PC479_001179"
     CDS             complement(45062..46465)
                     /locus_tag="PC479_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2803733.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(46729..47220)
                     /locus_tag="PC479_001180"
     CDS             complement(46729..47220)
                     /locus_tag="PC479_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2803734.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(47246..48058)
                     /locus_tag="PC479_001181"
     CDS             complement(47246..48058)
                     /locus_tag="PC479_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2803735.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(48071..49030)
                     /locus_tag="PC479_001182"
                     /pseudo
     CDS             complement(48071..49030)
                     /locus_tag="PC479_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(49148..>49510)
                     /locus_tag="PC479_001183"
     CDS             complement(49148..>49510)
                     /locus_tag="PC479_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2803736.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT        15800 a         9154 c         5303 g        19253 t
ORIGIN      
        1 attgcataat tattcaatac ctctctttgt atatttacat tatccatatc tagttatcta
       61 tttcaaccca atattagact acctactaat aaatttattc tctacatcat taacaataat
      121 tttgcactaa ataacatcat tagcctatct ctgatgcatt ttctttaatt ttatttatag
      181 ttttaatcaa tttatttctc tcttcaatat gattttcaaa taaatcaatt atacttccaa
      241 cagttctaaa tgggtcttcc atcaacactt tgttatcctc tataaatcca ttcttgacta
      301 cataatcgac tataagttta acaaaatgta tttgcttagt attaaagctc ttattattta
      361 aaaactctga aaaagcttca tttgcagctt ctctatccaa tcccactagt cttctcacta
      421 attgacttac tggagtatct ccaaactcct tcttataatc atcacaatta cctaactgtt
      481 ttaagagtat ttcctccaac gtttcaacat cttgctttgt tatcttttta ttatttctta
      541 atttaaatat tgctaattca tctttatggc tatttaaata atactctacc ttctttttat
      601 aattcttaag attgtttgca ttatatacag aatcatttct atcttctgct acaatcatat
      661 cttcaaaact tgtataatat atcttttgac tagacttttc aagatactta ataagctctc
      721 ttaatgatat acgtacttct tctacctcaa atatatctga atcttcccaa aaattctctg
      781 tcattgcttt ttcaatagta tatttttgtt gctgaatctg tggtatagtc ccaagttttg
      841 aaagtttttc tgctgtagcc attacatgcc ttataccttt acttgcatta ttattttgta
      901 agtatgcaag ttgtatatta tacataacta catcaaatct tttagccaat tcatcatcac
      961 tatatgaagt tatgagtggt gatatatatt ctcttatctc attttgtgct actgctccaa
     1021 tattgctcca aacattttca tttttatact tatgtacata acttaaattc atcctaacta
     1081 tataactatc ctcatttaaa gcttttacag aagttataag ttcttctaac aattcttttc
     1141 taagttctgc atactcacct tctatatatt taatatcttg aagttcttta accatactta
     1201 cctttatatt aaataatttt tcagtaagtg tttctgcttt atttccctca aatcctttag
     1261 gattaaattt aaaaaattca aaattattac aaaaatcaaa tattaagaat ctctccttat
     1321 ccatatcaac acctaataaa tctggacata atctagttcc tctacctatc atttgccaaa
     1381 acttagtctt acttctcact ttcttgaaaa aaacaagatt tagtatttca ggtatgtcaa
     1441 ttcctgtatc aagcatgtct actgaaactg ctatttgagg taatttattc ttatcagaaa
     1501 aatcatctat tatagaatct acataattta tactatagtc aactgctttt acaaagttcc
     1561 caccatactt tggatacagc ttattaaatc tttcaactat tgccctagca tgctttgtat
     1621 tctttgcaaa aattatagtc ttaccaatct tttcttctcc ttctattttc aacccctctg
     1681 tcattagctt atttaaaact aaatctattg tgtcagaatt aaatagccac tcatttactg
     1741 cattattccc tatttcgtct ccaatactct catcatcatt aaatgtattt tcatattctt
     1801 ccttctcttc atcagaaagc tcatcatact taattccatc ttccataatc ttagtcttaa
     1861 actcaatact agtataatct actaaatatc catcctcgac tgccttatta tactcataag
     1921 catatgtagg tactccattt tccatgtcaa aaactgaata agtattttta tctatctcat
     1981 cctttggtgt agcagtaaga cccattaagt aagaatcaaa ataatcaaag attgacttat
     2041 actttttata aatacttcta tgcgattcat ctattataat taaatcaaaa tgaccaggtg
     2101 taaacaatct ctttccatcc ttagacttcg tatcatcaat tgcattcatc attgttggat
     2161 atgtagaaaa tatcatccta gcatcttctg ggctgtcttt gctatccaat aaattacata
     2221 acgccaagtt aggtaatagc ttagtaaaac tttttttagc ttgctttacc aatgcttttc
     2281 tatctgctag aaataatata tttttaatcc aattatgtct tgaaagtaca tcaactatag
     2341 atatagcaat tcttgttttt cctgtaccag tagcacatac caaaagagtt ttgcgttgct
     2401 tattttcaag ggcctcacat acagcccgaa tagcttcttt ttgataatat ctattggata
     2461 tttcatcatt tattgttaca gaacttaaag acttcttact ctttcttcta tccatcataa
     2521 gttgtaattc tgatttttta taaaatccat aaacttttct atctgaatag tcatcccaaa
     2581 tatacatatc aaaaccatta gtcaaaaata ttactggtct ttgactatat ctcttttcca
     2641 aacaatctgc atataattta gcttgttgtt gaccaatttt tgggtctttg ctcgttcttt
     2701 tcgcctcaac tactgctaat ggcttaccat tttcaccata aagaacataa tctacatatc
     2761 ctacaccact ttcattaggc ataccttgta cttctatttc ctcccctata tcttttccaa
     2821 attcccaacc tgcaagcttt aattctatat ttatatatct ttttcttgtc tcaaattcgc
     2881 ttacttcgtc tatcttgaaa tcatagttta cagtattaac ttttctcttt tcagttagtg
     2941 actttcttaa ttcctcattt tcttttatta cttcttcaag ctttttatct tttgaactta
     3001 atctctcgta aagattttta agttcgtctg gtctcgtcct tttctcttct ccatgaagta
     3061 ataaatcttc attaaagtct tctgctgtat attcatctga ataacaatag tctatccaac
     3121 taacaaattg atgcaaattg ttaagtgaaa gtattgcttc tcctctctct atgttcgcac
     3181 tagtatgtac agataaatta cctaacttaa ctatatattt taataaaggt aacatatcat
     3241 agtctataag ttctataaaa ctattattat gaatcaacgt actgagatta tcttgataag
     3301 gcaatactaa atcactatca ttagcatata gccattttac tgcaagctct aaagctcttc
     3361 ttgttatagt tgcacaagta gatggactaa ctagtatact tttttctgct tctaagcaag
     3421 cagtattaaa actattgaaa tttttcttct tttgtaaaaa atcaaaatta cccaaggctt
     3481 ttatccccct tttgtccctt agttaaaata aatctctcta aaaactattt cttacagctg
     3541 tttcttatac taacaaatac ttcttcattt tctgtaaaca attcgtacat atcttcaaac
     3601 tgtggtatat catatccatg agaatcatca tttctcaatt ttctaagtgt ttctataact
     3661 atagaagaat ttttaggtaa taaattgtga tactcacaag ctaaaaggta gtcataaaag
     3721 tttttagatt ttatgtttaa atcaattgat ttataataca acattgcttt tgttaattct
     3781 agaaatgata ccatatagct tttaaagcct tcttttatgt atctagccca taactcaggc
     3841 atttcttcct tatcatataa gattttgaag ttttgcaggt catacaagca agaaccaaaa
     3901 tccctattta cttcttctat tgagttccaa gataatttgt ttctaattac catacatgtc
     3961 cctccaatct aagatatcta ttacatcact gttttcaaaa tagaatttat ttatataatc
     4021 cttaaaatct ttagattcat aaataacttc accttctaaa atagcttttt taagtgctat
     4081 tgtataatta tctgtaacaa tttttaggtc tacatcaatg tctaatatat ttcttagagc
     4141 ttctgtctta gttgcaagtt ctgtaattgg tacttttttg atgggaatcc aagctatgtc
     4201 tatatcactt tctgatgtat gtttatctgt tccataacta ccaaatatta tgattgctag
     4261 agtattttct tttggaaata catttattat tgagttttta atctcatttg gtatatttct
     4321 catttaatca cctcttgttt attaggtaat attgtttagt taaataattc tcctttgaaa
     4381 gctttttgca ttaatgaatt aaagttatct tctaactctt ttaagttggt ttctattttt
     4441 aattttaatt tatccacttg cttaacaaac tcagaaaact gattttgaaa ttcaattgga
     4501 ggaaccatta ttaaatattc acctaactta ctaactgata actcaatttg attcgttgaa
     4561 ccattaacac attttctata tattgtgtct tgtatatttg taaattcaaa taacgctgca
     4621 aaataatctg aatttaagtt atttgaaata cttcttaaaa ctgtaacatg actatctgct
     4681 atatattcag aattatcaat tttgttaaat tttacacatc ttcctaatgt cccagttcca
     4741 gtagaattta ttaatatatc attttgtttc aaaaatatat cttctatctt ctcttttaag
     4801 ttttcatcat aatatttaca attttctacc ttcaaatttc tccagtatat acatttttga
     4861 tttataactt ttatatttga ttgctctaca tatttaggag atacacctct ttttaaatat
     4921 atagaaatct ctttaatctt cttcatatca aatttttttg tattattaat tggctcccca
     4981 aacatctcaa taaatcttga tttaactaat tcatccaatg cttctatttg ctcttttctt
     5041 ttatttatta actcttgagc tttatttaaa acttctacca ttttattttg tatacaaatt
     5101 gtgaaatatg gtatttttat atcatttaac ttgcttttat ttaaagttgc acccataact
     5161 gctctatcag tattattaaa atttaaagtt ctcaaagcat aatataaata ttcagatgtt
     5221 ataagttcat tattttttat ttgaaagtta gcaattgctt catttgtaaa catgtcttct
     5281 tttaatatgg caactctacc tatagacaac ttaaaactca ttacaacggt atttttaggt
     5341 actaatttca tattagcttt ttcaacagct aaatctgtta tctcttcttt tgattttgat
     5401 atatattttt ctttcaaatc agaaattgat aaccatctat taccacttcc ccaataatca
     5461 gaagtatttc tagatggtgt tttgccaata tttatatagc acaattcatt taattttata
     5521 tactccatac cctatacctc taacattctt tctaattctt ctaatccaac agctatttcc
     5581 ttctctaact ccctaactct acctataatc acactaggag catcataaac tacttcctca
     5641 tacactacct ctttatactt attaatagat aaatcatacc cattctctct tatctcttcc
     5701 acatcaacaa aaaagctttg ttctgttctt tttctatcta cttccccatc aagattatca
     5761 aatctcttaa taatatcctc tatatcacta tcctttatct catttctctt atcatccaaa
     5821 gaataaccat cagctttcat atcatagaac cataccttat cagttccacc tgtccctgtt
     5881 ttagtaaata tcattatagc tgtagatact cctgcataag gcttaaacac tccacttggc
     5941 attgatatta tagcttccaa tttatgatta tcaactattt cttttcttat cgatttatgt
     6001 ccacctgttg aaccaaacaa tactccatct ggcactatac atgcgcatct tccaccaatt
     6061 tttaaaactc ttaaaaaaag tgccaagaat aataactctg ttttctttgt cttagtcact
     6121 tttaataaat ctgctgacac tgcttcataa tctaaacttc ccttaaaagg aggatttgct
     6181 aacaccaatg taaacttatc tttatctgta ttaacctccg ataaagaatc cttatactct
     6241 atattaggat tgtcaacgcc atgcaacatc atattcatag cacctattct aagcatagtt
     6301 ctatccatat caaatccata gaacatacca ttattaaaat gctccttcag cccttgcact
     6361 aagaaaagac ttgaatgatt atcccttaag tattgttgtg atgatactaa aaaacctgct
     6421 gaacctgctg ccgggtctac tataatatct tctggtgttg gtttaataag tcttactatc
     6481 atatctatta tgtgtcttgg agtcctaaat tgaccattag tacctgctgt agccactttg
     6541 gataataaat actcatacaa atctcccttt gtatctctat ccttacttaa ttctaaatta
     6601 ctaataccat caactatttt agataacata agaggcgtag gaattttaaa tattgcatct
     6661 cccatgtact ttgcataagc tgagtcttta tttccatgta atttttttat gaaaggaaac
     6721 acttcatttt gtactatttc atacattttt tctgaatttc cttcattaga aaacttactc
     6781 catcttaaat gttgatgttc atcatcaaat atcctttcaa aagaaactcc caaaagagtt
     6841 gcttctgctt cattttttgt ttctacttca tccaatcctt taataaataa aagatatgta
     6901 aattgttcta taacttctaa tggattagtt aatccaccag tccaaaatgt ctcccaaatc
     6961 ttgtctactt tgctccttaa ttctcctgta atcatatatg actttccctc cagtgttcat
     7021 aaaataattt aaatatatat tttagtcgta cgccttaata attagtgtct tatattaaat
     7081 tcaaaatttt actacacagt tattaaatta tttttcctat ttacatgttt tagttaaaaa
     7141 actaatgcaa tactcttatt ttatgattat tatacatccc atacaaaatt attttatcat
     7201 atatccagca tagaagtcat ctacctggat aactcttacc atattataac tttttaatat
     7261 taatttaaac ttagaaagaa attttttatt taattatatc atcttcctta tttcaaattt
     7321 ctcaattaca atctcaatca aaaaataaaa taagatgctg tctcaaaata gatataatct
     7381 tcttttttat tttcggaatg atgaaaatat atttaatttc atacaaaaaa gagtgcctcc
     7441 tgaactaaaa agttcatttt gagacacccc ttaatttaat cttacttatt ctctcccttt
     7501 attattaaca cttcactatc tctattgctt ttcctacaca tccacaaact tccatcctac
     7561 tcttaacaaa atctttaact gactctaaac cttttttacc atatttctta gggtcaatta
     7621 cctctgggtc agaattaagc acatccttaa ccccattgct ataagcaatc cttaattcag
     7681 ttgcataatt aaccttgcaa atccctcttt ttatagattc cttaataact tcatctggta
     7741 ttccagaccc accatgtaat actaatggaa ctgatacaac atctctaatt tcagataatc
     7801 tatctaaatc aagctttggc tctcccttat ataatccatg tgccgtacct attgctactg
     7861 ccaaagatga tacccctgtt ctctctacaa actcttttgc ttctaatggg tctgtatatg
     7921 ctcccccatc accaccatct aaatcgtctt ccttacctcc aactttacct aattcagctt
     7981 caacaggtat atctgaaggc atacatgcat ctacaacaga tttagatatt gaaatattat
     8041 cttcgtaact ttcatgagaa ccatcaatca taatagaagt atatccaact ctaagtgcct
     8101 gcatagctag tccaaagctt gaaccatggt ctaagtgcat tgctactgga acacttgcct
     8161 tctctgatgc tacctttaca ttcgctaaat aataatctag ccctgcatac tttattgttg
     8221 aaggtgttgt ttgcaatatt actggtgatt taagttcttc agcagcctct attactgcca
     8281 taaccatctc catattttca acattaaatg cacctactgc ataatgtcct tcttgtgcat
     8341 ctaataatat ttgtttagtt gtcactaatg ccataatttc tcctcctata tgtttatact
     8401 gatttgattt cttcgttatt taattttgct actatttctc tacttgtttt gctatttaat
     8461 aactcattta aaaattcttc attcattaat tttgaagata gttgagataa tgtttttaag
     8521 tgtaaattat caccattatc tgtagctgct atcataaata taatatgtgc taagcttcca
     8581 tcttctgatt caaagtcaaa cccttctttt gatacaccta ctgctacacg tggtaccttt
     8641 actgcatctg tttttgcatg tggtatcgct atccccatac caaaacctgt agatgatatt
     8701 tcttctcttt taaggatttc ttctttaaac ttctttttat ctaaaagaac actagattta
     8761 tcaagtattc caatcatttc atcaattaca tctgttttat tttttccctt aacatctaaa
     8821 attactgttt cttcgctata taaagagata ttattagata cgttttccga ttctgcactt
     8881 tcatttttat gttcatcttt tgttacattt gtattttcta tagtattttt ctcatttact
     8941 actttaggtg taaccatcat ataaagtaca gcacctacta atgaacctac aaatatacac
     9001 cctacccata aaagtggctt attgactaat cctagaacca aaaaccctcc atgtggagct
     9061 ggtacttcta ttttcattat ataagttaaa attgatgata ttgatgatgc aatcataagt
     9121 actggcaaca cttttaatgg attttttgct gcaaaaggta ttgccccctc tgttatatga
     9181 gtactaccta gtatataatt gattaatcct gctgctctat cttcttctgt aaatctattt
     9241 ttaaataaag tagatgctat tgctattacc aatggtggtg ttatacaagc tgcagataca
     9301 cctgccataa ataaataatt tccctgtcct agaagcatag ttccagttac atatgctgct
     9361 ttatttactg gacctcccat atcaaatgct gacatacatc ctattactat tcccaaaatt
     9421 atagggcttg aattttgtaa ccccgataga aagttcatca tagcattgtt aagtgctgaa
     9481 catggcccac caagtaacaa cattgctaca cccacaagac ctacgctgac aataggaatt
     9541 ataaaaatcg atttaagacc ttctaattgt cgtggtaatt ttcttaacaa atgtacaaat
     9601 agtttaacta agtaacctgc taaaaatcct gatatgattc cacctaaaaa ccccgaacct
     9661 gtatcaaatg atatcattcc tccaacaagt cctgctaaca taccagctct tcccgatata
     9721 gattgagcta taaatgctgc caatacagga accatcaagt tcatagaata tccaccaaca
     9781 gattttaatg tagctgctat ttgattatat tgttctgaat ttgggtcaaa tgaataaatc
     9841 ccccataaaa atgatatagc agtaagtact ccacctgcta ctactaaagg taacatgtga
     9901 gatacaccat tcattaagtc tttatacaac ttttgaccaa acgataaatt tccttcttct
     9961 gcaattttac tgcttgataa gtttcctttt ctaatagcta cttttccatc aatacatctt
    10021 tgtataagtt cattagcttt atgtatacct tctttgacag gtacttctat tactggcttt
    10081 ccattgaatc tgtccatatc aacattttta tcacaagcaa ctattattgc atctgccatt
    10141 tcgatatctt ttgctgttaa cttattttct acacctatag ctccattcgt ttcaactttt
    10201 atgtcacacc ctaattccct ggctgaattt tttaatgctt cttctgccat aaaagtatga
    10261 gctattccag ttggacaacc tgttacccct agaatcattt tctttcccat ttttatacca
    10321 cctttctgac tcttatattt tttatataat cttccacatt ctccaaatta cctataccat
    10381 tactttcagc cacacttgct cctgttgctg ctgacctttt taatgcttct tcaatatgtt
    10441 ctggattttc caaccatata cttaaaaaag cagctaatgc agaatcacca gcacatgcag
    10501 agctgactac tgtaactggt tgagcactag cataataaat tgattttcca ttataaaaat
    10561 aagaaccttt ttctcctaaa gttagcaata tattttgagc accttttaaa tgtagtaatt
    10621 taagtacatc cttaatatct tcttcatctc ttacaataat tccaaatata tctttgattt
    10681 cttcatcatt tggttttatc aaatatggat tatactgtag taactccttt aattttggtg
    10741 aacttatatc gagtattgtc tttatacttt tatttttaca aattttaaat atatcctcat
    10801 agtaattatc atcaatacca ggaggtagac ttccacttat tgataagtaa cttatatctg
    10861 ctaatgactg tatcttatct agcatatcta gtttttgttt ttcatttaca tatgacccag
    10921 agtttacaaa tttaaattca tcacttccat catttaaaaa tatatttata cgagttgtat
    10981 cttctaccca agtaggcaat acgtcaaatc ctctcttttc gctttcttca actatatatt
    11041 ttcctgaaaa tcctccaaaa aatcctaata tcttagattc tataccataa tgacctagta
    11101 caaaagttac atttaatccc tttccattag gagaatatac agcatttgat gtacgattta
    11161 ctaattttct ctttatccca ttagtagata tattcatatc tattgcagga tttgtagtta
    11221 atgtatatat cattgtcttc ccccttgctt gtgctgtgta tgttttcatt ataataaaaa
    11281 agtctatgaa aatcttttca caaatagaaa atcgattttc atagactttt caatttaatt
    11341 ttcagaatta ttatatctga ccatataatc tataattatc ctagatataa tatacaaggg
    11401 caatctggaa gatacttcat attcagaaat tgatatatgt tcatgtttgt atcctacaat
    11461 atataaatcc gaatacttta taagttttgc actttcacta caacagcaag ttataacctt
    11521 agctccaact aaattggcat tctgtgctga ttctacaagc tctatggtat ttccattcaa
    11581 tgaaaatata attataagtt cgtctttttt tattgattta gattttattt tcattatgtt
    11641 ggggtcattt ataaaaacaa cattaaaatc aagtaattgc aatttaagac taaactcttt
    11701 tgctacatat tcagtaagcc ctcttgctaa aatatatatc ttactagatt tatttatagt
    11761 ttttattata ttgattagtg tagttaaaga tatttgttct ataactctct gtgcttctat
    11821 aaaggattta tttattatat cccccatatt ttgtatatca tcattttcat cttttactgt
    11881 taatctatac ctaagttcat tgaatccatt taaaccacat tttctaattg ctcttgaaac
    11941 ggttgctgga gatgaatatg tttcatgagc aatctctact atagataatt ctggtaatat
    12001 tgattcattt tcatttataa atttaattat ttcaagttcc gtcttcgtta gattttcaga
    12061 attacttttg tttagctcaa ttatcatatg ttatctcccc ttatataagt tttatttgtc
    12121 taaatttatc gagtaatata atatattacc tcttaactac ttatttttat atacaattaa
    12181 gatttagtca atgtctatat atgtaaattt ataatataat aattttttgc ttaaagttta
    12241 ttatgttatc ttatagcatt ttaaaataaa aaagaattgt acctctacca aaatttggat
    12301 gaagtacaat agatattcct catactgata tgtttatgat taaaagtata aatataaatt
    12361 tcgctatgaa tcatataatt aataaatcat tttatttaca attataaccc cccttataaa
    12421 actatattaa taatatatac agtatttata aggggggttg tttatattta aaaactattt
    12481 acttttctct taattcttct ccattagttc ttattacatt ttgataccaa taaaaactat
    12541 cttttttata acgttcttta cttccatttc cattgttatc tatatccaca tatataaatc
    12601 catatctttt ttccatttct tgagatgaac aacttactat atctattggc gaccacatac
    12661 aataagctaa tatttcggtt ccatcagcta tagcttcacc tatttgttct atatgttttc
    12721 ctaaataatc tattctgtaa ctatcatgaa cttttccatc ttcaaattta tcatgcattc
    12781 caaatccatt ttcagctatc attattggct tttggtatct atcataatat tgagataaag
    12841 tattgtataa tccttttggg tcaattgccc acccccaagg atttgctttt aaatatggat
    12901 tctttatatt gtcagttgga ttcatggtgt ttttacttgc tgatactgtt gtagaataat
    12961 agtatgataa agctagaaaa tccatagtat tttcttttaa gattttatca tcaccttcct
    13021 ccataactat atttatgtta ttttctttga aaaatctctt aatatagact ggatactctc
    13081 ccctaaaact tacatcagtg aaaaaatatt gtagcctatt tctttttaaa gcaaatacta
    13141 cattttcagg gtcacaatct tctggataag atgtacaatc tgccatcatc atacctatca
    13201 tcatatctga atttatttct tttccaagct tcttagtcaa agctgtagct atgaattgat
    13261 gatgaatagc ttgatatcta gtctcttcta cattatcaac ttggtctaca caaaatgcaa
    13321 ctgaattaaa tgattcataa tgtattaaat ttatttgatt tataactatc caatatttaa
    13381 ctttattttt atatctattt agtattaatt ctgcatatct aacaaagaaa tctaccactt
    13441 ttctattaga ccatccttta tattttaata ctaaattcaa aggagtctca tagtgaagta
    13501 gtgttacaat aggttccata ccattatcta ttacttcatt tataagatta tcataaaact
    13561 ttaatccctc ttcattaggc tctaaatcat ctccatttgg aaatattcta gcccaattta
    13621 ttgaaaatct aaaacacttt aaccctagct ctttcaaaag ctttaaatct tctttgtatg
    13681 tatgataaaa gtctattcca ctacgcttag gataatatcc attattatct ttcatagcaa
    13741 actttattct ttctatagtc atatcagact cataaggttt agatatatct ctatctttat
    13801 catacatatg tacatcagct aaacaccaac ctttgccacc ttcattccat gcaccttcta
    13861 attgattagc tgctacagct cctccccata aaaaaccttt aggaaactta cccaatttaa
    13921 tcactcctta ttttatttat ttaaatcatt tactattatt taccttatat tctatagact
    13981 ttactattcc tactaaagac atagttatat ttaactcgct gaatatggtc ataagagtat
    14041 cttgagcatg tgcaaataac agcgaatgtg ctcttttctc tccaccagct tccccttgta
    14101 taatcttggt ttgagctcta tgtgcttctg tcataaaccc ttttgcttta gctagtttta
    14161 aatcagcatt tctataatca aacttttcca aatcattaag agcatgttta atttctgttc
    14221 tagcctcacc tgcacgaagt attatttgca ttgctacttc tactaattct tcatctatta
    14281 tatgttcttc aattgtcata tttataattt cctttcaatt tctaaattta aacctcttga
    14341 ctctctaatt ttatctcttc tttctcataa gctttaaaaa atggatacca tatcataaat
    14401 agcaatacta tatttattgc taaaacaata agacctctaa catcttgatt taccataaaa
    14461 gtagatatac caaaaggaac ataccataaa ttaaacacct tacttggaat tgtaacaaat
    14521 cccatactca ttgataaata agtagttatt gatattacta ctgttcctat ccacataggt
    14581 aacattaaaa atgggttcca tgccactgga gctccaaata ttactggttc atttatattc
    14641 attatagaag ggacaataca tgctcttcct attgctttta ttcttccaac tctggataaa
    14701 cacatcatta taactaatgg taatgtagca cccaaccctc ctaaagttat ccagcctgtt
    14761 tgaataactt cgtaaacata tatattagtt gctactcctc ctgatgctac tgctgctgca
    14821 ttagctgcaa ttgcaccaag ttgaattgaa taagttatag gagatagtac ccacccactt
    14881 attcctaaag aatatataaa tactactaag aagtttaaga gtacaaatcc aggtaatgtc
    14941 tgtgctatat ctgatactgg tttaaatact ctaactatta tttcaaacat gtcaaagttg
    15001 agtccataaa ttaatcccca gcctacaaac attatcactg ttataggtaa catattatca
    15061 aaccacttgc ttacaaaatc cggaagtgct gaatcttctc cgaaaaatga aaaagaacca
    15121 aataaagaca ttattaatcc agagaataat cctgcaacta gcgatacaag cattccttct
    15181 cctccaaacc tgttgaagtt aaatatagct ccttcattcg taatctctgg acgaatcatc
    15241 attaacatta atccagcacc tgcaagacca gctactacac tcatactttc aaatttctta
    15301 ttcttcattg tttccatagg caataaaaat gctataaata atcctaaaag tccaaatgta
    15361 tattgattta ttggacttaa atcaggtaaa aagcttatgt aattcttcaa gacagaaact
    15421 atagttacta atgagcctac aaataccatt ggcaaaactt tcattatagt tgattgaact
    15481 gattgtaccc atatattctt agtcatatta tttacaacag gagtaaattt ttcacttaaa
    15541 aaatctataa atttattcat aaacactctc cccataaata ttttatttgt ttgatattag
    15601 ttctagtgct tcttccaaag cctttgcacc atctagtctt ccatatgcag ttttagatat
    15661 acatgctact ggaacgttat attcattagc cttttgttga atttcttcct tcatataatc
    15721 taaatgtggt cctagtaata aacaatctat ttctgataaa tacttatcta tctctgactc
    15781 acttctcgct tttatatctg cttctatacc ttttttcttt gcactctttc tcatactagt
    15841 tgccataaaa cctgtacttg ctcctgaacc acacgccaaa agcactctta tatttttcat
    15901 aataatacct ccaatatttt tatccattaa tttatttctt aatgaacttt ttattttatt
    15961 atgtttatat tttacttccc aaataagttc atttcacata aatatatttc ctaataatag
    16021 gaaatcattt actcttaatt agacctatta atatagtata atgtatctta taatacgtac
    16081 attcaattat ataattatca tatcatacag attttatttt aaaactatgc gagggaatct
    16141 aatgaaaaca tctaaaaaag agctattttt aaatttcata aaaacgcaac atgattggat
    16201 agattcatcc acattagcta attaccttaa tgtatcaaca cgttcgatta gaaaatatgt
    16261 aaacgagatt aattctaatg gtgcatttat attatcttct aaaaagggct ataaagtaaa
    16321 tctcaatact aattgtcaaa ctaagattga tagttcagag aatatatcac ctgataacag
    16381 attaaattta attttaaaag aacttatagt taatagtaat ggaattaata tatttgattt
    16441 aagtgaagaa ttatttgtta gcccagctac tattgaaggg gatatagtca aagctaataa
    16501 atttatagga agttataatt taaagataaa gcaatcgaaa tttttattga aactagttgg
    16561 aaatgagtct gcaaagcgca agttaatgag ctctataata tttaaagaaa cgggaagtga
    16621 ttttttaagc ttatttgatg tacaaaaaat atatcaagag tataatttga ctaaactaaa
    16681 agaaaatata atatatatat taaaaaaata caatttattt ataaatgagt atgctataaa
    16741 caacatactt cttcatttaa tgataactat agacagaata aaaaagaata attacataga
    16801 ctctgttgaa gtagtaaact acatagacaa taacgtagat ataaatattg caaaagacat
    16861 atcaaatttt ttagaatctg aatacaatat aactttaact agtgcggaat tatactattt
    16921 agtatttcag ttaacaaaca aaactacagt cttaaactac aatcaaatgg atactaaatc
    16981 attgtcaaat tatatagatg aacactttgt taaattaact aaaaaaataa ttaaaaatgt
    17041 atatgactta tatcttatag atttatctga tgaggaattt gttgtaaaat ttactcttca
    17101 tgtaaaaaat ttaatcagta gagccaaaaa taatcaagtt ttaagaaatc aaatacctca
    17161 aaagttaaaa gattcctacc ctttgatata tgatatatct gtttatattt gcaatcagat
    17221 tcaaacttta gaaaatgtag atatagacga agatgaaata tcttatatat ctcttcatgt
    17281 aggttcattc tttgatagac aaaaattatt agaagataaa gttctatgtg cattaattac
    17341 tccaaattac tatgatttac aattcaaaat agtaagagac cttgagaaga gatttaatga
    17401 atccatagaa attattcaga ttttttcaga tactcataat cttgattttg acaataaggt
    17461 agacatggtt attactactt taccaattaa cgatagatgc cctatacctt ttgtttatgt
    17521 aaatccttac ctaaatagaa aggattatga taatattcaa agtaaattta ttcagattaa
    17581 agatagaaaa aatatactta ctgtacaaaa tcatttagaa atgtacttta gtgaatcttt
    17641 gtttatgaaa aatatatatt tagatagtgc taaagattat ataagattta tgggaaatat
    17701 tctgtacgaa aataaatatg tcaaacccaa ttatatagat gatgttctta taagagaaaa
    17761 aatgtcttca actgcattta ataacaatgt agcaattcct catagcatga aaatggatgc
    17821 tttaaaaacc ggagtatgtt taattgttaa cgataaacca gttaaatggg gtgaggaaaa
    17881 agttcaaatt atagctatga tacctataaa tgaaaaagaa aaagaaaaat tcaattacat
    17941 atttgaaagc tttattgaaa tattatctga atggaataat gttaaagaac ttactaaggc
    18001 agataactat agtagtttta tgaatagaat agcttatctt attcaaaata tctaaatcca
    18061 ttaatttatt tccaatatat gtttcttaag ttattaacat aaaatagata agctgtcaca
    18121 ctaaaaagtc taaattaata tgtgacagct ttttacctaa tataccttgt tttaatacta
    18181 catatttttt gactgttgta tactagttat aagattgaat atttgtgttg ttgtagctgt
    18241 catattctca ctagcagttg ctttatccat tgcttcttct attgtcatag ctctatttac
    18301 tatagaaaaa taagcatcta taccctcttc attacattta actgcatctt ctgttgtggc
    18361 tccagccaaa cctattactt ttaccccata tttttttgcc agttttgcaa ctcctattgg
    18421 agccttgccc atagcagttt gattgtccaa tctaccttca ccagttatga caaaatctgc
    18481 atcttttaac tcttcctcta atttaatttc ttctaatatt atttttattc cagattctaa
    18541 ttttgaatct aaaaatgcta aaaaagcaaa acctaatcct cctgctgctc ctgctccttc
    18601 tacattagct acatctttac ttaagtaatt tttaactact ttggaaaaat ttctaagccc
    18661 attatctaac ttctcaatta tttctgatgt tgcacctttt tgagacccgt atatgtatgc
    18721 agctccattt ttcccaaaca gcggattatt tacatcacag gctattttaa acttacattc
    18781 atctaattcc tttaatctat tttctatttt tatatgtctt atttcattta aaacttttcc
    18841 acctaatcct actaacttgt tatttttatc ataaaactca aaccctaagg cttgaagcat
    18901 acctatccca gcatcattag tagcacttcc acctatccca actataaaat ttctatagcc
    18961 tttatttatt gcttccttta ttatttcccc aacaccataa gttgtagtgt ataaaggatt
    19021 tctaagttca gtcggaacca atgttaatcc tgatgcttga gctatttcta ttattgctgt
    19081 acttgtattt tttaataatc catatgttgc atccactttt ttagctattg gtccagtaac
    19141 ttctatagtc tcttttttac cattcatccc ttgaactaaa gcatctactg tcccttcacc
    19201 accatcagca agtggcaata tctttacttg cgcatcttct ttaaccttca atatcccctt
    19261 ttttattgca tttcctgcct ctattgaaga taagcttcct ttcaaagaat ctatagaaat
    19321 taatattttc atacttatct actccttttt attactcctt attttaaaca tataatttta
    19381 taagcttaat ttatctttta ttttatactc tacatctctc tttgtataca acttatacca
    19441 ttaatattct tttatcttat aaaaagggag tgtctcaaaa taaatttctt agttcatgag
    19501 acactctttt gtataaaatc aaatatatgt ttattatttc aacaatgata aggaggatta
    19561 tctctatttt aagataattc ctttttagca ctatctaatc atttttagca ctatttaacc
    19621 agtatagttt taacttggtt cattaagcac tctacattta cagagttatt atctattgat
    19681 atagattcta attctttttc atttaggtta atccatttag ctgttttaat atttttgtta
    19741 aatactatgt tagctgattt tgtttcttct ttagaattat aaaatctaac tactaatgcc
    19801 tcttcttttt cagccttttt tacaacactt aacacaaggt ccttattagt ttccttaagt
    19861 aaactatatg aataatctac ttctatttct gattgattca atttcatagc attatatggc
    19921 attttattat aagtttgtat ttttgttaaa tactctttag ccatatttgc aacatttgct
    19981 tctagtgtag atttttcatg agtaactatt gctaagtcta tttttatatc tcctatcatt
    20041 tgagaatctg gagtaggcaa ctttattcct gatggacgac ctgggcgtct tgccatttct
    20101 tctttaccta ggaatcctac acttctaaat aatgttatag ctattgtgtc aaatttttcc
    20161 cctactattt caaactctct tgtactgttt gtaagaacag ctactccatg ctctttgtca
    20221 gacaatccaa cgaatgttag cattggatat atagaatctg gtctttcact ccagtcttcc
    20281 tgttgccaaa cttccatagc atcatcatac acacttctct ttatatgtcc aaactgatta
    20341 tcagacacag aaaactttga agctatattt gttggtatat aagttctaac cctacaatct
    20401 cttgcttggt tatttatgtt gaatactaca tctattatag ctttatcatt tggaacattt
    20461 ataactatat caaagtcaat aaatgaatct gctctattat tttttctagc ttgtatatct
    20521 tttggtactt ttaatctata atttatcttt atctcattat taaatctatt tttctttata
    20581 gtggaatttg ctactatatt atcgttaaat attaatttct catttggaag tggtgaaaaa
    20641 tcatactcat ctccttcatc tcctccattt tccatcaata atacattttc aaaagtttta
    20701 tttaattttt tatcaaatat ctttaatgtc ccgttttcat taacttgaat agtataaaag
    20761 tcattatcta ccatgtcaca tacttcataa gctttttcta acatatgttc aacttctttt
    20821 atcatatacg ccttataccc cattgcaggt attttatctt taaagtttat tgtatatacc
    20881 ataaatgaat catagtttcc ataatgaaca atttgacggt ctattaatcc tgcatctatt
    20941 acttctttat gtaccacttc aaaatccaac ttattattat ctttatcaaa tatttcaaat
    21001 gctttcattt tagttataac ttgggcgctc actatttcat tcctatcgta aggcattaag
    21061 ttaaatgcta ttaacttatc taaagccatt tcacaactca ttgcatcaac tattcttctt
    21121 ttgtaaaatg ttattaattg gtctactttt tcctcagcta agaaaaatct attcattatt
    21181 tcctggtgaa ctttatctga acaacaacat ccaatgctgt catgagcatg atttttcatt
    21241 atttctttcc aaattaactc tattaatcca tgatgatatt caaatcctag agaatatgca
    21301 atagaagcta gtggctccaa tatgttagtt atctttgatt ctattctagc atttgctgat
    21361 tttatatcca ttcttgttga gaatatactt ctatgaaccc tcatatattt cccatctaaa
    21421 aattcccctt ttattgtatc tatatcctca tttttttcta gctctttaaa tatgttttca
    21481 tatctactta agaaaaattt tctgtctgga taacattctt ttaatttata tataacttca
    21541 aatatattct tttgaattgg catttggtca tgtccatttg gaagtattat atggtcagta
    21601 gttgcaccct tatcaagaac cggtaaatat ttatccattc ttgttcttag ctcatcttca
    21661 ttcattggta gatattttcc tattgcataa cctaatggaa gtatttgaac caaaacagaa
    21721 gacccatcat cactcttcca attaaactct gttttattag ttccttttct ttcagaacat
    21781 cctctccaaa acattgaata ttttatatca aacccattta gtatttgtgg catttgagca
    21841 ctttgaccaa atgaatctgg taaatatcct attttcatat attctccaaa ttcatcacaa
    21901 tcttttattc catatagtaa atttctaagt atggattctc caccaactac catttcatca
    21961 gtttgagtat accaaggacc tattattagc cttccttctt gaactaattt ttttattctc
    22021 tccttacatt caggcttaac tgctaaataa tcttctaata tagcagtttg accatccatc
    22081 acataatatg gatagttatg attattttct aacatatcca ttatttcttc catattattt
    22141 acaagtaata ttctagattc ctcagttgag aagtaccatt ctctatccca atgcatatga
    22201 ggaactatgt gtacattttt tttcattgct tatcctccaa aacttatctg ataatatttt
    22261 ctgtctaatt aaaaatccaa taaaaatact actgagttta caaaattttt taatatttat
    22321 tttatcacta taattaaacc tttgatttaa atatctaata acgtatcttt taatgtatct
    22381 ttctttgatt tttttgtttc ctagttatta ttaataatat tgctgatatt atagctccaa
    22441 gtactgccgc aaagaaccat attgatgcta taataaatcc tgacatactt ccctctaaaa
    22501 gcgccaatga gaatatcccc gctccaggca cttgaagacc tatattgaag aatgctacta
    22561 tagacccagt tatggctgaa ccagctacca atgatggtat aacacggatt gggtcctcta
    22621 tcataaatgg tattgcacct tcagttattc cagccaatcc taatatccaa gtttgctttc
    22681 ctatttcctt ttctgattct gaaaataaat ctctgcctaa aacagtagct attgtaactc
    22741 caaaagctga aaccatctta actgatgcaa atattgcata aggtataaaa tttccacttg
    22801 ctattgctcc tattgcaaat gtataggctg ctttatttat tggtccacct aagtcaaatg
    22861 aaaccatagc tcctattatt gcacctagta gtattgcatt tactccagtc atcccattta
    22921 accaatttat aagaccattg ttaagtgctg ctacaggctt tcctactaca aataacatca
    22981 ataaacctac taccaaagaa ccaactactg gatataccca aaaacttacg aatccagcta
    23041 atgtgccttt ggctggtata ttcttcttta aaaattttac tatataacca gcaagtaaac
    23101 ctcctaacat acctccaaga aatccactac ctattaaatc agctgctaga cctgctgcaa
    23161 atcctggtcc aagacctggt ttttcagcta gtgagtaaga catatatgct gataatactg
    23221 gaaccatcaa tgtacctaac atacctccag aaagctttct taattgccat aaccaagaac
    23281 cttcttgatt gtatagttct tgtagaccaa atgcttgaga tgctaatact gcaaatgcaa
    23341 gtatcatacc tcctgccaca ataagtggta ttatatgaga tactcctgta agtattgctg
    23401 attttacatc ttttttccat gatgattttt cacaatatga tatatcagat gatttttcta
    23461 tataatcacc tttaccttgt ttttgtgcta cttctaaagc ttcgtatatt atttcttttg
    23521 ctctttttag tggagctgct actgacgttt ttatgtttgg caaatgttca aatctttctg
    23581 tatttttagg tgctacatct gttgcatata ttattgcatc tgcccttttt attatatctg
    23641 gcgtatgagg gtccaccata ccattagctc cttgcttttc tacatacaca tccacaccta
    23701 tttctttacc agctttaatt aatgcttctg ctgacatata tgtatgtgct ataccggcag
    23761 ggcatgctgt tattgctaat ataatttttt ttgattcatt ttgaatccta atctcttctt
    23821 ttatttgttc tttcttttct tttatatcta agttattaaa taactctttc ttagtttttg
    23881 aatttaacaa catattcata taatcttcat ttgacagtct agtaactaac tcagaaagta
    23941 tttgtatgtg tgtatttcca ccttcacttt ctggtactgc tattaaaaat attagctcta
    24001 ctttattatt tgggtctacg ctttcccaat tttctattgg tgtctctaag gtcatagctg
    24061 caaatgcagc ttctttaact gacttagatt ttccatgagg aattgcaagc cctctttcaa
    24121 aacctgttgg agataaagtt tctctgtaca ttacagcttc taaatattcc tcataagatg
    24181 ttaacttgcc ttcatcatat agctttcttg ctaagaactt tattgcgtct tcctttgttt
    24241 ttattttttg ttttaaaaaa acaaggtttt catttgtgat tttcttaaat tccaaaatta
    24301 cgccctcctt aaatcattta atgctatgaa tatagcataa aataaattat gaaaatgttt
    24361 ttttgaaatt atacacacaa tttcttctta aaaaataaag atttgtgtat ttatacacaa
    24421 atctctatct taaccaatat tgctctatta ttgctctcat aatcatcata attggaacct
    24481 tatctgatat attgtaccca tttacttgtt taattgttga atagcaatat aaacttatat
    24541 ctgcaatttg ttgcaattga ttttcacata tatgtgttaa agctactacc ttacaaccct
    24601 tattaactgc tgtctttgct gcttctataa cttctttcgt ttctccactt aaactaagtg
    24661 caaaaaacat atcttgttca cctaattttc ctatatcatg aagtacttca tgtctttgac
    24721 tatagtaagt tatattatca tgcccacctc taatttcttt tactaatatc tcacataaag
    24781 gaatgttttg acctattcca taaactataa tactcctaga tttttgaatt atatctgcta
    24841 ctttatttat tttttcataa tctattagct ctaatgtctt ttttatatct gattcaaaac
    24901 cattttttgg ctctttatcc tcatttttta actcttcttt taaattattt tttagttgag
    24961 agaaaccatc atactccatt tttttacata gcctaactat ggtatttggg acagtaaaaa
    25021 atttatctgc taaagattgt attgataatt ttactatttc ttctttgttt tttctcatat
    25081 attcaattat catgtcttct gtatctgtaa atttatactc atattttttt gctctttcat
    25141 aaaattccat agtttctcct ccatctattt tattattcat aagtaaaatt caaaaatatt
    25201 agctaaaatt ttcgttaata taattataat ttaatatttt taatttatta atgcatacta
    25261 ctctttttat tatacataaa aaatgtgtac ttcctatatt gatagagatg attcaagttt
    25321 ttattttgat acatctctat caatattttt ttagaaaata cacatttttt tcaaatactt
    25381 atttttaatt aaaactttag gtaatctatt ctttatatat aattattaga cttgaaaata
    25441 tctttaaaat caaccaattc aatattattt ctttttaacc acattttaac ttccttatca
    25501 catagcattg catgttcata tactctctct tgaataagag aagatgattt tataagacta
    25561 taatctataa atcctggatg agtaaccatc aatgttatat ctttatctaa atttatatat
    25621 ttttcttcaa ataatttaac aaaagattta tttaacttgt aaaaatcatt ctgaggatat
    25681 ttacttggca tgctaataca tgaatccaaa taatagggac aacttggtat accgtattct
    25741 tttgcagcat cacacattgc tgatattgat acatcttctt ctataccatg tatatcaata
    25801 tactcaggca atttactcat atacaacttg taagtttcaa tttgagcaca aacttcttca
    25861 tatacttctt cataattaaa aacctcttta cctgtcagta gttgcgttct tcttattttg
    25921 ctagaaacaa attttccacc ttcatctaac aaattcttta tcttaatatg ttctttgcaa
    25981 caaggttctc ctattactaa attaacatgt aaaccaaaac atacatcatc attttttacc
    26041 atttctatag cttgtcttga aaaaggcata ttcaccatga ttccagcact tgtaacaata
    26101 ccgttcttat aagaagatat tatccctagt gtaacacctt ctgaatatcc tatatcatct
    26161 gccctaacta ctaatttctt cattttatgc atctgcctgc tttctcaact catcaagttg
    26221 catttccatc tcatccttta ctttaacaac atccgttcct atgatgattt gaactgatgt
    26281 attactcaat ttaattaccc ctttggctcc tgttttttta atattatcat cattcaaaat
    26341 agtggcatcc ttcacttcta aacgtaatct cgtcatacaa ttattaagag tcactatatt
    26401 ttcataacca ccacaatttt taattatctg tttagccata tatgaaaaat taccatgaga
    26461 aagttttaaa ttcttttctt tttcactaac tttatcacct atctctatct caacttcacg
    26521 tcctggtgtt tgcacattat cttttattat tatatattta aatgttacat aatataatac
    26581 aaagaataca actcctacag gtataactaa ccacgcttta tctccataac taaaacttaa
    26641 gacatagtca attatacttg ctccattaaa tgaacccaag cgagcaccca ataaatatag
    26701 tacaactcca cctagaccag aataaaaaga atgaattata tataactttg gagatgcaaa
    26761 taaaaacgaa aattctattg gttcagttac tgttgccata aaagaagtca atgctccagc
    26821 ggtcattgca ccttttactt cttgcttctt tttatcttgt gctgttttat atatagcata
    26881 agaaatagca ggtattgaaa acatcataat tatgtaaaat cctgtccaga agaatcctgc
    26941 atttgggtct cctgccataa atctaggaat ttctcctctt ataacttctc ctgttgatgt
    27001 tgtatattca cccatttcaa ataatatgta agcatttaaa acttgatgta acccagttgg
    27061 tattagtgca cggttacaga ataaaaacaa gaatattcct attgcaccag ctcctcccaa
    27121 ccaaaaacca aattgattca ttgcagcttg tggatatatc cataataaac taaatatata
    27181 tgataaaatt aaagcaacca ctggtgatag tgtaattaca catttttctc ctgcaaagaa
    27241 gcttaaaaat gctggaactt taactttata tgttttattg taagtccatg ctatacaagc
    27301 tccaacaatt atcccaccaa atacacctgc atttaatgta tcattcaata actctaaatt
    27361 tgacttaaat atcatatatg caactacggc tgcaaccatt acacttgtct tgtctttaga
    27421 ctttgagtag gcactagctg ctcctaataa aaataagtag tccacatttg aaaatactat
    27481 attagatata ttagatatag ctggcacatt gagcatattt ggaccaaata ataatcctaa
    27541 tatacctgct gctggcatta cagctattgg caccagcata gtctttccta aaagtgacag
    27601 ttgttgcatc attttgttta agaatctctt catatccaat cccctttcta tttcggttta
    27661 attttttgtg aaaacgattt attcacattt tatattttaa atattatatt tgtttaaaca
    27721 aattgtcaat attttttatt cattttattt ttatcattta gtttaacata taaatccttt
    27781 taagtgtatt gctatatcta gtctattttt ttaattcatt ttttttattt taaaaatatg
    27841 tttaaccatg tatttgaaac aacccacaaa ataattctat tttgtccttt ttatggttga
    27901 tatgattcta ttttcactat acatgtctaa acacatttga tataatttgt tcatatcagt
    27961 tcaataatat tactaaagac ctttaaggag ggataggggt atatggctaa aaataaattt
    28021 tcagagatat atgaagtatt gaaagaggaa atacttgatg gtaaatatac aagtaatatg
    28081 atgctgccta ctgaacttca acttattgaa aggttttcat gtagcagaaa cactgtacgt
    28141 agagcaattt ctcaattaaa cacagaagga tatgttcaaa gtataaaagg caagggtgtt
    28201 gtagtgttag aaaatagttg ttctaatgat ttttttctaa atatgcacaa tttcaaaggt
    28261 gtagaatcga ttgtagaaga taaaaaggtt aatactgcta ctagtgttct ccacttcagc
    28321 aaaatcttaa ttgataataa gttatctaaa aagacaggtt ttaaagttgg tagtgaagta
    28381 tattaccttc acagactaag atatattgat aacataccta aaattctcga cattaactat
    28441 tttctatgta gtatagttaa agaccttgat gtttctatag cacaaggctc tatttataaa
    28501 tatatagaag agtgtctagg tacaaaaatt gtttcatcaa gaaagatatt taaaattgaa
    28561 aaagctacag aacttgagtt aaaaacgcta cctctaaatg attataattg tgttggtgtt
    28621 attaaaaata gtgtatatac tgatgatgga aagctttttg aatatactga atcaaagcat
    28681 acacctgaaa catttgtatt catggatgtt actcaaagat attaaaaaaa ttatgtttaa
    28741 ttaaacataa tttttttacg tttagacact ttatcccata ctaacctcac tattctatat
    28801 acaattatat gtttatacaa ataattattt attgtatatt ttagctttaa tattagttta
    28861 acgcttgaca atttgtttaa acatattata attaacttat aaattacagg aggtatcaag
    28921 tatgaaaaat tggtggaaaa aagcaactgt ctatcaaata tatccaaaaa gtttcaaaga
    28981 tagcaacaat gatggtatag gtgatataaa tggtattata gaaaaattag attatctgta
    29041 ttctttaggt gttgatttat tatggttgac tcctatgtac gtttctcctc aaagagataa
    29101 tggatatgat atagaagatt attataatat agacccaaaa tatgggacaa tgagtgactt
    29161 tgaaaaactc ttaaaagaag ctcataaaag agacataaaa ataatgatgg atatggtcct
    29221 aaaccataca tctactgaac ataaatggtt taaagaatct aaaaaaagca aagacaatcc
    29281 atatcgtgat tattatttct ggaaagatgc taaacctgat ggttctgttc ctaataactg
    29341 gatatccaga tttagtggaa ctgcttggaa atatgatgaa accactaacc aatattattt
    29401 acatttattt gaagaaactc aagctgattt aaattgggaa aatgaaaagg ttcgtgaaga
    29461 atgttacaaa atactagaat tttgggctga taaaggtatt gatggttttc gtttagatgt
    29521 agtcaaccta ctctccaaaa cacctggatt acctgatgac cctataacag gaccaaaagg
    29581 agatggaaga actcactacg ctgatggtcc tcgtatacac gaatatttac ataatatgaa
    29641 tcaaaaagtt ttcaaaccta aaaatatagt tactgtaggt gaaatgtcat ctactacacc
    29701 tgaagagtgt atcaactata ctagagaaaa tcgagaagaa ctatctatgg tttttagctt
    29761 ccatcatatg aaaacagatt ataaaggagg cgcaaaatgg actaatgaaa tgttctctct
    29821 ggataaatta aaaaaagcac aatctgactt tcaatataaa atgtatgaag gaaaaggatg
    29881 gaatgctcta ttttactcta atcacgacca acctagagct ttatctagat ttggagatga
    29941 tagatttttt cgtcaagaaa gtgctaagat gttagccata acattatttg gtctacaggg
    30001 tactacatac atatatcaag gtgaagaaat aggtatgaca aatgcctact ttactaaaat
    30061 agatgaatat gatgatgctg aaagtaagaa tgcatactat catatgatta aatctggtat
    30121 tgatgaaaag gaggcattgc ttatacttca acaaaaatcc agagataatg cccgtactcc
    30181 tatgcaatgg gataatacta tttataaggg gttttctaat cataaaccgt ggttaaaagt
    30241 taataatgac aatattagtg tagaaaatga gcttaatgat tctcgttcag tattttatac
    30301 ttatcaaaga ttaatcaaat atcgtaaaca atatgatatt tttacagaag gtacttatag
    30361 attgttagat gaaaatcata aaaatctatt tgtttatgaa agagagtata aaaaccaaaa
    30421 tcttcttgtt ataagtaatt ttactcatga taatgtagta tataaattac ctgaaactta
    30481 tttgaataaa ttaaactaca caatacttga aaccaattac actagagata taattgaaaa
    30541 taaaatggaa ataaaacctt acgaaagtat aatgatatat tataaataaa gttatttatg
    30601 taaaaatcgg tataacaccc tggtgttata ccaattttac taaattcaaa gtaatattta
    30661 tagaatatct atagattaca ataattattt gacttctaca tcattatatt tctttgaata
    30721 tactttagtc atatagaaat atataggaac tacaattatc atcccaccta atccccaaac
    30781 tatcttcata ggttcatcaa tacctgcttt tatcagtaat aatatactta taataactgc
    30841 aaatattggt ataattgctc ctccaggtat tctaaaattt acatctttac cagcatactt
    30901 ctttctcaat attagtacag ctaaacatgt aggaatatat tgaataaatc tagatacaac
    30961 acttatttga gataatgttg tgaatgaacc cgaccatgct attagtaaag ttcctatgac
    31021 agatactata attgctatat aaggagctcc atttttatta gtttttctta tagacttagg
    31081 cattaatcct tgttcaacca aagcagcagc agaccttggt gtaaatatgg atgatgcaat
    31141 atttatacct ccaacagata ctatagttcc tattgtaatt atatacccac ctataggacc
    31201 taacatctta atggcagcat ctgcaacagg agtagctgac cctgctaagc cattacccaa
    31261 cacccctata cttattccca gtataagcat atatattact gatactacaa acattaccat
    31321 taccaatgct ttaggtacat ttttcttagg gttctccata tcttttgctg caacagctaa
    31381 agattcaaat cctgtaaaag cataaaataa agttatagat actgctacaa aagctgatga
    31441 cgcactttga cctttttgta ttattacaaa tggggtaaaa ttagaaccct ttataaagaa
    31501 taatcctaca gctataaata aaactattgg taataatttt cctatagtca tgatattatt
    31561 taagtgctta gattgtttca tccccataaa atttataatc ccaagtccta caactaatat
    31621 accaattatt atatttttag taagtggttc tgctgcttgt ggccatacag atgatagtgt
    31681 tacagcaaag aaatttgcca tagtagccca agcaatcata ctaactatcc acttcataaa
    31741 tccaacttca aatccaacaa aatctccaaa tgcctctttt gcataaagat agggccctcc
    31801 atcagtatca aaccaactac ctgcttcagc aaaacataaa gcaatagaaa cagctaaaag
    31861 catacaaaaa actaatacta atatacttgc tggacctata tctgtataag catcaccagg
    31921 taacccgaat atacctgacc ctattattgc attgattcct aatagtacaa tactccaaaa
    31981 acctaactta atgccttttt ccattgttat tccccccaaa gccaacttaa tatatttgta
    32041 ttttttattt agatgcttct ttaactattg ccataaatat ttttttcata ttatcataat
    32101 ccctagtcat catttctgga tgccattgta tccctattac aaactcactt ccttcctttt
    32161 ctatagcttc aattaaaccg tctttagatg tagctgaaac aatatatccc ggtgctactt
    32221 tgttgacaac taagtgatga aaactattaa ctaaaacttc tttttctcct aatatttcat
    32281 ataattttgt cccctccttt atctgtactg tatgtgtagg tacattagat agatgttgct
    32341 gattgtgttt aatataagca ccttcaatta aagataaatc ttgatacaaa ctacctcctt
    32401 cagttacatt tataatttgt tctcctctac atatacctaa aactggtttt ttcatatcta
    32461 aagcatgttt taataatttc atatcaaaac tatctctctt tggtgatatt gcccccaact
    32521 tattatgagg ttcttctttc catatgagtg gatttacatc ttgtccccca gatagtataa
    32581 gcgcatctat attagatacc tgctccttta taatctcatc atcatataca atcggtacaa
    32641 tatatggtat agctttacac ataactactg attgtatata atcattattt acataagccc
    32701 tttcatagcc aggaaacatt cctccttcat caattagtaa attacctgat attcctatta
    32761 ttggatattt ctttttagcc aatttcttca ccccttaact tatttgtgaa caataaaaaa
    32821 tgctacttct cttaaagagc aagtagcata ccaagatttt atttatttga acattataat
    32881 ttattttaga aatacttttt ttgaatacga gtaatataaa taactttatt tacttatatt
    32941 ttattttaat cctgtttagt ttgacttttt taaattgttt taagtacaac taataggtaa
    33001 aataggcatt tagattttcc tttttatcaa cctattaaat actgtttaat atattttttc
    33061 cccttctatt taattcactt ccctttctac cagattcaca tattatcaaa tccagctctt
    33121 ttaacatatt aagtattccc cttacctcac cttctgttat attgtagcct aattcattta
    33181 ttgaattaac aataccattt ctacctatat ttttattaag attattggca ttttttaaac
    33241 actccattac taactttact ttctctatag atgttctatc ctttaatata cttatttctt
    33301 tactaaaatc ttcagttata tttaataagt tatgaggtaa atcatttatt aaaataacat
    33361 cctcagaact cataatgtta atatacatag cagtattttt taattctctt atattacctg
    33421 gccattcata tgcattaata atgtcttgaa catcttgact aattgctctc ttccttttca
    33481 taaaatgctt taacataact tctatatctt cttttcgctc tcttaaaggt ggtatattta
    33541 tgggcaatac atttatccta tagtataaat ctgctctaaa tctatctcct tttatcattt
    33601 ctaataaatt tttatttgta gccgcaatta ctctaacatc tatatctatt acattttctc
    33661 ctccaaccct cattacttga ttctcttgta atactctaag taatttgctt tgtaagtata
    33721 taggcatgtc tcctatttca tccaaaaata tagttccatt gttagcctgt tcaaataatc
    33781 cttttttacc tccttttaaa gctcctgtaa aagcaccttc ttcatatcca aaaagttcac
    33841 tttcaagtaa attttctggc atagctgcac agtttatcgc tataaatggc tgactatgtc
    33901 ttggtgatgc attatgaata gactgagcca tgagttcttt tcccgttcca ctttctcccg
    33961 ttataagaat tgtataatca gattcagcta ttttcatacc taatttctta gttctttcca
    34021 tcttcgttga aactgttttt atatcatcaa aagtatatct agcaacttgt cccttttctc
    34081 taagcttctt tgataggttt tgctccaatt ttcttatgta agttatttct tgaatacaaa
    34141 aataatatcc agtaaccctt ccaaaactct ctatattata cttatttacg tttaagtact
    34201 tattattaaa ttttataact tcatcatgtg ccttactcaa actaaaaatc tttttaatat
    34261 tctcattctc taattcatct atatttttcc ctataatatt actttgaata tctagtatat
    34321 cccctaaact attattgcaa ataattatgt ttcccttatc tgataccatt gccataccta
    34381 agtttgataa atttattata gaatttaatt catctgcttt tatagtaagt tctttatatt
    34441 tagtatttat tcctgaatat aaactaatta tttcatctgt atacttaatt agcctttcag
    34501 taatttcttt atcatccagt ttgagttttg ctattatttg aataaacgtt gaactatcaa
    34561 tatacctatg gcctaaatca ataacttctt ctatatattt tggaactaaa cttgcctctc
    34621 ctggcgttat agctatctta atatttctat attcttcatt gggattgtat ggtataaaat
    34681 tcagatgatt tattcctata ttgtataaat tagatattgt ttccaataca gtatgcttat
    34741 tatcgtttac tactaataca tctattcctt ctggcaatga aaataactta tatatatcct
    34801 tttgctttat gctcctattt atagttatta tctttgagac atcttctaca tattttttta
    34861 tttttaatac tctatcatct atcattacca atattatgtc atcttttatt aattccttct
    34921 ctgttatttt atttaaataa taatttttta tcattacttt atctttaaat atcatttcta
    34981 gatttttatt taagaaacat tctaatttag acaacccatc agttactact gctattgttt
    35041 tcatttgatt actcctttta aaatatactc tactctttta taatatacat ctgattttca
    35101 ctttaacagc acaaaaaatt gtacatttag tatactaaca tattccttta ttagttttat
    35161 gtccctaaat atcatagttg caatatctat catatttctt aaacttctat aaagcaaaaa
    35221 ttgtgccaaa ttaataattt tcttaatttt attctaaagt tcctcaatag tatttacaga
    35281 cttagcactt atagcttata agtctttata ttattaatct atttttatag gttaattgta
    35341 caaatcatat atcactttta cccattaaat gcattataaa aaagatggaa taacgattcg
    35401 tcactccacc ttttttgtaa taaaaaattg tatatataaa cactatttac tactcttcta
    35461 agtacaaaca ttatctacat actataacct ttcaccatta gattctatta cttgtttata
    35521 ccagtcaaaa ctctttttct taatcctaga caagtctcca cttccatcat catacttatc
    35581 cacgtatata aacccatatc tcttggccat ctcaccagta gatgcactta ctaaatctat
    35641 acatccccac ggagtatatc caaataaatc aacaccatct tctacagctt ccttcatttg
    35701 ctctatatgt gcttttaaat actctatacg atagtcatca tttatcactc cattagattc
    35761 ttttttatca taagctccta aaccattttc aactaccatc ataggtattc tatatctatc
    35821 ataaatttca tttagtgcat atctaagccc ttcagggtct atttgccatc cccattcact
    35881 agacttaaga tatggatttt tcactccacc taatatgttg ccacttgtat tatcttcttt
    35941 agaatcacta cttacacatg tagacatata ataactaaat gtataaaaat ctacacaacc
    36001 ttcttttaaa atttctaaat ctcctttttc catttttatt tcaatattat tttctccaaa
    36061 atatctattc atataagttg gatattcgcc acgcacttgt acatctccac agaaataatt
    36121 cattactcta ctatctcttt gagttttaac tgcatcatct ggattacaag tataaggata
    36181 cattgttgca aatatttgca tacatccaac ttgataattt gaatctattt catgagctaa
    36241 cttaacagcc ttagcacttg ctacaaattg atgatgtaac ccttgaaaac gaagttgagg
    36301 tatatctact tggtttataa aatctgtagt cccttcattt aatattcctt gtgataagta
    36361 gcctcccata ggcattgtag ctgaatttat ttcattaaat gtcagccaat attttacttt
    36421 tcctttatat cttttaaata ttgtttcaca gtaattcata tacaagtcta ttacatctct
    36481 tgaaacccat ccattgtatt tctctgttat tgcaaatggt atttcatagt gagatattgt
    36541 tattaatggt tctatattat attttaaaca ttcatctatc acattttcat aaaatttaag
    36601 tcctgcttca tttggttctt tctccattcc agttggaaat attctagtcc atgctattga
    36661 aaatctaaat accttaaatc ccatttctgc aaataaagct atatcttctt tgtatctgtg
    36721 ataaaaatct attgcttcat gacttggata aaatgttcct ttttctataa ctctcgttat
    36781 tcttttactt actttttgag aacctccagt acacatatct gaacaagaag gacctttccc
    36841 atctaaattc catgctcctt cacattgatt tgctgctgtt gctccacccc ataaaaaatc
    36901 ttttctaagt gccataattc tacctccaat aatttattta tagtaacttt attttaaaat
    36961 aaaaatacat atatataatt cttaattaat agaactctct tttacgctaa aacagttatc
    37021 aattcctgac tattatttac atgcttttgt gtattttcta aaatatccac ataatcagaa
    37081 taattagtaa caactactgg tgtttctaag caatatcctt cttttgaaat actttctatg
    37141 tcaaaagtta ttaatatctg acctttttta atcttatccc cttgattaac aaaagatttg
    37201 aaatatttac cttccaactg aatagtattt aaccctatat gtatcaatag ttcacaacca
    37261 ttatcagata taattcctat tgcatgtttt gttggaaata aagtcattac agtcccatca
    37321 aatggtgcca ctacttttcc ttctttaggt gttattgcta caccttttcc caatatacct
    37381 gttgaaaacg ctgcatcttg tatctgctcc aaagccttta cttcaccttt tattgggcta
    37441 tatactactt ctcttttgtt tttttctata acgccttgcc cattatcatc tttagaaatt
    37501 tctactatag catcatcttt ccaaaaaaat aatgttaaaa caaatcctac tacaatagct
    37561 actattattg ctattatagt tgcataaaca cctttcatat ctccagttgt tggatttata
    37621 taattaggta taccaaatat tccaagtcca cccattacat aactttttac attcattatc
    37681 cccattacag ctcctgccac accaccacct acacagctat atatgaatgg tgtttttctt
    37741 ggtaaagtta ttccatatat tgcaggctct gttacaccac atatccctga aataactgca
    37801 ggtatacaca gctcttttat cttcttatct tttaatttaa agtacattgc tgctactact
    37861 gctgtttgtg caaaagatgg tacaaatgct cccactaata ttgtgtcaaa ccccatagtc
    37921 ataaggtttg acatagctat tggtactaat ccccaatgta gaccaaatat tacaagcact
    37981 tgccacaata aaccagttaa tgcaccacat aaaattggtg aaaagttaaa taaagctaaa
    38041 aatccatttc caactatatt agaagcatag gtagctatag gccctataac cataaatccc
    38101 aatggtaaag aaactaataa aactgccatt ggtacaaaga aattctttac tacatctggt
    38161 attattttag caaacacttt ctccaactta gctgctaata atactattaa aattactggt
    38221 actactgttg aagtgtagtt cattgctatt actggtaatc ctaagaaagt tatatatact
    38281 ggtgattcaa atattgttcc agtaaataga ttgtataaag gttgacctgt agatgctaga
    38341 gtatctaatt gaatacctga atagcataaa actgcaccta taagcattcc aataaatggt
    38401 tttaatttga atttcttagc agctgtatat ccaagaatta ttggcatata ctggaacatt
    38461 gcatctccaa ttgcatttaa cataatatat gcaccatcag tttctccata caaacccaat
    38521 gctgtaaata ctgcattaaa tccttttatc ataccagcag cacacagaac tcctaatatt
    38581 ggttgaaaaa ctcctgatat tatgtctata aacttattaa gtaatccttt tggcgcatct
    38641 gagtctgaat tatctattgt atctcctcct aatccagcca aatcgcacac aacttcataa
    38701 acatctggaa catgattacc aataactact tggtattgac ctgcactttt cattacagta
    38761 actataccat ccattttttt tagcacatca tcatttgcta ttgactcatc ttttaattta
    38821 aaacgtagcc tcgttataca gtgagataaa ctgctaatat tatcctttcc tcctacattc
    38881 gcaatgattt cttttgatag ttgttcatac tttctcattt tttctcctcc attttctttt
    38941 tgaaatatga aggtttggcc aacttaatgt caccacccaa tatatataaa cctaattatt
    39001 aggatttata tctgacttaa tacttttact taccactctt tcaacgtgaa tagttaagta
    39061 taatttttcc tcatctgtta aatcgtagtt gtatttttta tgtataaatt catccaactt
    39121 ttctatgaat ttataagcat ttttatattt aacttttatt acatttaata aatcatcttc
    39181 attattttca tgtaaattat tactaacaat tcgttctgca aaatacttta agtgagtaat
    39241 aaatctatga taataaactg aatcttcatc aaatactatt ctgtaatggt atttaaccat
    39301 atttgaaatt tcttgtatta tttgtgtaat ctcatatata gtttttatac tttgatccat
    39361 ttgtgcatta actatatgca gtgctataaa tccagcttca tcttctgata gtctaatttc
    39421 aaattttttt tctatataat ccaatgcctc taaccctatt ttaaattcac tcttataaaa
    39481 tctttttata tcccaaagta atacattttt tacacttact ccttcacgag ctctttctat
    39541 tgcagtatac atatggtctg ttaaagaaat ataaatacta tcatttaatc ttttccctag
    39601 tttttcttta gcatataaaa ttatttcatc tgataattcc atatatctta taggaatatc
    39661 tgctattagt tctttaaatt tatcagatat atttgggtct gaaagtttga atatcttatc
    39721 aattttatcc tttgatatat gctctcctgt acgtttttta taagctaaac ctctccccat
    39781 aactactatt tccttatgat tttcatcagt tgtaataatt acattattat tgagtatttt
    39841 ttcaataatc attgtgatgc cccctttatt agcattaagt taaaataaat cttttttaat
    39901 tatttagaat atttaaaatg taatttatta cataaaaaaa aacctattta ttacacatta
    39961 aatagactcc catgagtcct gagtaataaa taggtttagc ttgtatttaa acaatcacta
    40021 tccgtttgta ttttatttta attattattt attatatgtt agcaaattta ttttttagtg
    40081 tcaacttttt ctcaatattt taattttaaa taaattatat aaattgaatc ttcaaagtac
    40141 tatttttatc aataaacaaa ataaaaaagg tattactttc tttaagtaat acccatttat
    40201 ttttaattta agtggttttt cttaatctca tcaagtagtc catcttctaa ataaatttct
    40261 aatgcacatc ctaccattgc ctttactgct ttgtgaagtt tatcataagc ttcctcagaa
    40321 tttgcataat ttagaaattc tttttcatga acatatacca ttggacttat atctaaggct
    40381 atttctgtat acatagtagg gcaaacttga cttacattac ctatatctgt agaacctact
    40441 ggaccatctt taccttctaa tatatctgtt atacctgcct caatcaaatt attctttgtt
    40501 atcttttgaa gtactttgtt atttattatg ttgtcaaaag aattttcaaa gtatctataa
    40561 gaaagcttag ctcctgtcat taactctgca ccttttgcac agtttataac ttttttagtt
    40621 acttcatcta aataacttct ttcagcagct cttacgaaaa acttacaagc tgctttttct
    40681 ggaactatat ttggagcctc tcctccattt gaaactatac catgtattct aacatctgat
    40741 tttacatgtt gtcttagtgc atttatccca gcaaaagtaa gttgtactgc atctagtgca
    40801 ttgactccca tatgagggtg actagctgca tgtgctgata ccccttcaaa actaaactct
    40861 aatgaatcta tcgccaaagc cttacagttt atattattaa aagcttctat atgcatttga
    40921 taacttacat caacatcatc aaaagctcct gccttaacta aatcacattt tcctcctgtt
    40981 gtttcttcag ctggtgttcc tattagtaca atttttcctt taaaattttc ttttaactta
    41041 cttaatacta tacaagctcc tagtgtagat gctgcaatcc agttatgtcc acaagcatga
    41101 gctggttctt tattttctcc atatccagga agtgcatcat actcagctaa aaatgctatg
    41161 gtaggtccat cattatctcc taattcagct ctaaaagcag tctccatatt acaatatgga
    41221 taaactgtat caaatccata ttccttcatt ttttctacta agtattttga tgatatgtat
    41281 tcttcttccc ctaattcagg attgttaaat ataaaattac aaacttcttc aatttctttt
    41341 ccaagttcat tctctatctc ataagctctc ttctttatac tctctatcat aatttacctc
    41401 ctactagaat cctagatata atcctattgt caaaaatgcc attacaagaa ttgttaatac
    41461 taccattaaa ggaattacga atctaaccca ctttgaccaa ggaatattgg ctactgctag
    41521 tgctcccatt aaaacacctg atgtaggtgt tatcaagtta gttacaccta taccaaattg
    41581 ataagctgta actatcattt gtcttgatac atctactaag tcgccaagtg gtgcaagtac
    41641 aggtattgta agtgatgcaa gccctgatgc tgatggtatt aaaaatgcta ttaatccttc
    41701 tattaagaaa gttaaattta taaatatagt ttttggtaat ccatttaata aatttgctgc
    41761 agaatttaaa attgtatcta ttataaatcc attttctgct attattacga ttccacgtgc
    41821 aaatgctata cacaatcctg ctgtaattaa atctcctgct ccacttataa atgaattaac
    41881 tatctcatct tgtttaagcc ctgatgctat tcctgatatt atacctatag ctgtaaatat
    41941 cattgcaatt tcagttatgt accattctag tcttaaaact ccatatatca taatcgccat
    42001 acctattgtg aaaatggcta ttaccatggc ttctcttttt gtaaattcct ttatatttgt
    42061 atcactatta accattaatt cttgatttag tgatatatca tgtactaaag attttttagg
    42121 gtctttttta actttattgg catacatcat aacaaatcct atagatatag ccatatatat
    42181 tataaacata acaactctaa actctattcc agaacctggt actatttgtg ataatgcttg
    42241 tgctattcct acagagaaag gattcgtagt agctgctcca aatccagctg ttgctcctat
    42301 aaatactgtg gccactgctg tcaatgaatc atatcccata ctggtcataa gtggaataaa
    42361 tatcatataa aaaggtagtg tctcttcact cattccaaac gtcgtcccac ctagtccaaa
    42421 tagaatcatt gaaatcggta ttattaaaaa ttgaaacttc ttcattttat ttacagtatg
    42481 agctataaca gcttctatag ctcctgtttt attaactatc ccaaaagctc ctccaactat
    42541 taaaacaaat ccaactactt cagctgcatc tataaacccg tttataggtg ctgtaaatac
    42601 atcagtaatt ccttgtggat ttgatggtgc tttttcataa gttccagcta caacaactga
    42661 ccttccgtct atatctactc tttcaaaatt tccacttggc actatccaag taagaattgc
    42721 cattaacact attagcaaaa atattattgt aagtgtctgt ggcatagtaa actttttctt
    42781 ttttaaactg ctcattttta atctccccac tttttaaatt aaatatttta ttattgcatc
    42841 cctttatcct attttgtata gatgtaaaaa agctcaacat ggatacatgt tgagctaaat
    42901 gtgctaaata caacatacct gcatcttccc tttatcttgt ttaagatagc tctccatcaa
    42961 aattcgggat aattttgatg acagtactgt acctattaaa tacagtccca gcactagctt
    43021 cggacactag cacttcggca cttactcctt ttatattgcc tcattggttt ccaacctctt
    43081 caatattaat cttagtaaat gcaacctcta cctcaatctg tttgttagat gaggtaattc
    43141 ttattaattt ttattaattc aaagtaattg tagcataata tgtcgagatt gtaaatattt
    43201 tcagtataat aattcttact ttatgatttc attccatttt tcacaaataa ttttttctcc
    43261 atataattta aatactgtag tatattttaa atacacacaa actttccaca aattttaaat
    43321 tattataata cctttataat aaaaaataaa agctatgtaa acttatcaat ttacacagct
    43381 ttatatacat taatctactc caagtacagc tttaagcttt gatgctattg taataactcg
    43441 tggaccaaat ataacttgaa ctgagttttt ctttttaaca actccacttg cacccaattc
    43501 atttacccag aagccatctt catagaatcc atcaattgaa tttagctcta ccctaagtct
    43561 agttgcacag tttgtaacat tttttatatt attacttcct cctaaacctt ctattataga
    43621 atgtacaagc tttgtatctt tatcatctac ttcctcttct actgcattgt tgtatgctgt
    43681 tttaccagca tctacagatt gtctttctct atattctttc ttactataaa gcttaacttc
    43741 ttctgaactt cttcctggtg tttttatatc aaacttcttg attgcccatg taaatattat
    43801 ataagtaact ataaaaaata ttggtatcaa aaataataat ggtaacgcat ccactttttg
    43861 tggttgtaat aaattaggaa acatatttct aaatgattct cccattatag aaacattaaa
    43921 catttctgta aatacatacg tcaatcctgc cagtggtaca tatgctacaa aatacaataa
    43981 tggtgcaaca aataggaatg tatactctat tggttccgtt acccctacca acatggctgt
    44041 tactattgct ggtatcaata tacttattgc ttttttctta ttagcattat cagccaactt
    44101 atacatagct aaagctgctg ctggtaatat tgcaaaatgt attaataaac gacctgttgt
    44161 gaagttacga gttatataag atagtgtatc tgggtctccc atttgagcta atcttatatt
    44221 attaacaccc tcgtaaattg ctccacctac ttccattgtt ccacctactc ttgtatattc
    44281 tatagggaat gcaattaagt gatgtatacc aaatggtaat aatgctttat ccattgctcc
    44341 aaaagtaaat gttcctaata atccactaga acctatccaa ttaccaagtt tagataatgc
    44401 caaccctact ggtggccata taaagtacat tgctgtaccc aatactattg atgctataaa
    44461 catcataatc attgaaaatc ttggacctga gaaaaaagat aatgcactag gaagttctat
    44521 gtcaacaaat ttgttatgta ctgcactagc tacaaatcca gctattatag ctccaaatat
    44581 actacaatca tatgaaaaga ttcctaagaa agaaccatat aaagaacttc gactatatgc
    44641 ttctgcctct cctaatccag atgccataaa cgcttctgct gtaactgtat ctgaatttat
    44701 accatttgct gctaacaatg tacttataac tgtgtgcatt cctataaata aaacaattcc
    44761 tcctaatgca ccccatcctt tttccttttt agataatcca aaggctatac ctgtaacaaa
    44821 gaatattggt aagtatctca ttaccataaa tcctaaatcg tttattactt taaagaatac
    44881 atttataaat gacccttctt ttattagtcc gctaagccct aatgcagaaa cgttttccat
    44941 atttgcaaaa gctgacccta atcctataaa tactcctgct acaacaagta atataacagg
    45001 tatcatcatt gctccagaaa gtacttgtaa tttatccttc atagtattat ttcctccatt
    45061 tttattgaaa tgccaggcta tataatgcat atatatatac ttctattgct ttgtataatg
    45121 tatctatact tatattttca tttgcctgat gtgaatttgg attatccccc tctattgtgg
    45181 gtccaaatgc aactccattt tttaacactc ttgcataaga tgcagctcct tttgtaaaaa
    45241 gttcggcttc ttttccaaaa ccttgtttat atgcatttga taatttattt attaattcac
    45301 tatctggttt tacatacagt aattttaaat ccttatatat atcaactatt aaattttctt
    45361 tttttccaat ttcattaatt ctattagtta taaactccca acttataccc ttaggatatc
    45421 taaagtctat tttcatattc aattcatttt tctctaagaa gatactcatt atacatattg
    45481 tagaaactcc catatctaca tcttctttat acatccctaa tttttttcca tgattatcat
    45541 ctgtaaagta tgaatttaaa atatccctta ttataacgcc tttatcattt agttttttaa
    45601 ttttctctaa atcttttgct aaactaaatg cacagttata tgacctgtgt ggatttctcg
    45661 ataaagccct ttctcctgta tatcttacta atactttacc ttcatctaat tcttctatct
    45721 ctgtataata agctaatgat tctactaaat ctttcttatc attagtttta aaaacagcct
    45781 caattttgtc acatacaaat ccatcttctt tgtttgactt tatatcaact aaattgtgat
    45841 ttctaaattt atctttatct attttctttc ttagattgaa gtataaaata cctttttctc
    45901 catttactat tgggaagttt ccatctggag aaaatgcaaa ttttggctgt tcattgacac
    45961 taaagtaatg ctccatacat tcccaagtcg tctcttcagc tccacccact atcaatctaa
    46021 tctttctctt cggcttttca ttaagttctc ttaagaaaag taatgcatat aaaatcccta
    46081 tcaaaggtcc tttgttgtca ttaactcctc taccataaag atagttatct ttttgacata
    46141 ctttaaaagg tttactcttc caaagttctt tattatatat tggcaccaca tctatatgat
    46201 taagcacccc tactacttct tctccttctc catattctat atgaattgca tatccatcaa
    46261 agtcttttac aacaaaatct ttgtcttttg ctatttgaat aagcttatcg aaggcatttc
    46321 ttatttctat cccaaatggt tgatttatat ctgttgtact ttcatctcta attgaaggta
    46381 tttcaactaa acttctaata tcatttatga tattttcttg attggatttt aaataatcaa
    46441 taattagttc tttgtttact atcatcttaa ttatttatca cccctttata ttaattttac
    46501 aaagcatttc agttcatatc tacattataa attttgaata ttattttttc aatatttcaa
    46561 atacttctct aagttaccat aatgatacgg taaacaatca acagattatg acaaaatcat
    46621 actataccta ttactaatat tttaacttat ctattacgtc ttttttaatg taagtttatt
    46681 ccaaaaatac ataaaaaaag atgtagcctt agctacatct tcttatatct attcaacttc
    46741 cattattatg ctattaatat ctacatttcc aggttctatc ctatctttta ctgttctctt
    46801 aacagaatca gcatcatcca ttataactac aggagttatt atatttaaat catttgacgt
    46861 tataaaatct aaatctactt ccattattaa atctttagct tttaccttat ctcctgcctt
    46921 taccttaaca tcaaacggct ttccttctag tgacattgtc tctaaaccta catgtattaa
    46981 tatatttgta ttatcattac tcttaatcat tattgcatgc tttgtatcaa atacattgac
    47041 tatttcacca tcaacaggag aatataactt accttcactt ggtattattg ctactccatc
    47101 tcccatcatc ttttgggcaa aaactgcatc tggaacatct tctaagttca ctaaatttcc
    47161 cttaaaaggg ctatatattt tctcaatctt attttctttt ttcttaaata tattaaacat
    47221 aacacactct ccacattata aaattttatg ataatatttt tcttaaagct tttgctacac
    47281 catcatttac attagtatca gttacaaaat ctgccataga ttttacttca tctgtagcat
    47341 ttcccatagc aataccaaga cctgcaaatt taatcatgga tatatcattt tcattatcac
    47401 ctatacaaat aacttctttg ctatctatac ctaatatatc acaaaatttt ttaactgcaa
    47461 taccttttga agtaccttta ctcataactt caaagtttat ctttccagaa cttactactt
    47521 ctaaatcttc ttgtttttta aattcttcct ttgcttctct caaagaatct acgttttctg
    47581 agaaaagtat acattttaaa atttcattat cagctttttc taataaagtt tcacaacttg
    47641 cattttctat tatttcaatt ctgtcttctt ctttattttt actatttatt aatctatatg
    47701 gatgttcttc acctatttca ttgttggata taactatttt acagccttta aaatgtttgt
    47761 ttaaattata cttttcacct attgtataga ttttctttag agcttctttg ctcaaaacct
    47821 tcttatactc atatcccagt aatttaaaat atgtcccatt agttgatata atatatatat
    47881 catcccctaa aacacttgta aaatatttca tagcattata aggtcttccc gtaactaagg
    47941 ctaccttgat acccttttcc atggcttctt ttagtgcttt aatattttct tctgatactt
    48001 caaatccttt acccatcaaa gtaccatcca tatcagtaca aattaattta tatcgcattt
    48061 gtatcacatc ctagtaagat ttttctaata tttcaacaag gtcttctttc tttccaaaag
    48121 gtaaaaatcc taaaactgga cagcttgtta tttgttctaa ttgttccttt gttacattaa
    48181 actcagacat ttttgtctta agccccactc tctttaaaaa actttctatt tcatttccta
    48241 aagttttagc tgcttcatca tcttccactt tttctaattc ttggtttagt attctcgcta
    48301 ctttggcaaa tttctctata ttatccttat agaacttttc tgcaaatttt gggaacacta
    48361 tagccaaagc ctcaccatga ggaatatgtg taactcctcc tattatttca cttagtggat
    48421 gtggtgctgc tgctccagca tttgccagtg aacttcctcc taatgtatct gctactgcta
    48481 actttgttct gtattctata ttatttttat cttccattac tttaggtaga tagttaatga
    48541 ctaattccat agatttttca gacattatct ctgtaaaatc attagcattt ggatttatat
    48601 aactttcaaa tgcatgagta aaagcatcaa atgctgttga tgcagtaatt ctctctggca
    48661 gagttaacat aagctctggg tcaactatac attcttttga gaaacaattt tgatgaaata
    48721 ttgtattttt ctcgcttcct ctcgatatta ctgctgcttg tgtaacttgg cttcctgttc
    48781 cagaagtagt tgatacagct attaatggta attctttttc acttacctta tcgtatattt
    48841 taaatggatt tgtatatgta ctaaacatat aatcccaatc tattttttct aaaccaaaag
    48901 tcaatgctaa tacttttgca gtatctatac ttgaacctcc tccaacagct aaaacaacgt
    48961 ctacagattc ttttttagct aattcaaacc cttcgtttat taattctaca gttgggtttg
    49021 gctctactct ctgaacctcc catgactaaa gtcacagggt tcctgcttca cagaattttg
    49081 catactaaaa tatgtcttac taattctcca caggccatcc ccgtagttcc tacggttctt
    49141 acataagtta tttattttga attgttatta gtcttaatcc ttcttttaat atatttatac
    49201 ttgcatttat atctctatcg tgagtttcat tacaactagg acaaatccat tctcttatat
    49261 ttaagtcctt cacttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa
    49321 agaatttacc tacctttaca atttgtctac catgccaatt agccttgtat tctaattgac
    49381 gaataaattc tgaccatgat acatctgaaa ttgagcgaga tagtttacga tttttaatca
    49441 tattctttac ttgtaaatct tctatgcaaa taatatcatt ctcttttatt aatttagtag
    49501 ataatttttg
//
LOCUS       DAMJYX010000019        48980 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJYX010000019 DAMJYX010000000
VERSION     DAMJYX010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.019"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            72..1268
                     /locus_tag="PC479_001184"
     CDS             72..1268
                     /locus_tag="PC479_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2803737.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1361..1885
                     /locus_tag="PC479_001185"
     CDS             1361..1885
                     /locus_tag="PC479_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2803738.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1910..2086
                     /gene="rpmF"
                     /locus_tag="PC479_001186"
     CDS             1910..2086
                     /gene="rpmF"
                     /locus_tag="PC479_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2803739.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2293..2850
                     /gene="fapR"
                     /locus_tag="PC479_001187"
     CDS             2293..2850
                     /gene="fapR"
                     /locus_tag="PC479_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2803740.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2861..3883
                     /gene="plsX"
                     /locus_tag="PC479_001188"
     CDS             2861..3883
                     /gene="plsX"
                     /locus_tag="PC479_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2803741.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3909..4895
                     /locus_tag="PC479_001189"
     CDS             3909..4895
                     /locus_tag="PC479_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2803742.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5004..5933
                     /gene="fabK"
                     /locus_tag="PC479_001190"
     CDS             5004..5933
                     /gene="fabK"
                     /locus_tag="PC479_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2803743.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5949..6899
                     /gene="fabD"
                     /locus_tag="PC479_001191"
     CDS             5949..6899
                     /gene="fabD"
                     /locus_tag="PC479_001191"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2803744.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6899..7648
                     /gene="fabG"
                     /locus_tag="PC479_001192"
     CDS             6899..7648
                     /gene="fabG"
                     /locus_tag="PC479_001192"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2803745.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7702..7926
                     /gene="acpP"
                     /locus_tag="PC479_001193"
     CDS             7702..7926
                     /gene="acpP"
                     /locus_tag="PC479_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2803746.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7955..9193
                     /gene="fabF"
                     /locus_tag="PC479_001194"
     CDS             7955..9193
                     /gene="fabF"
                     /locus_tag="PC479_001194"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2803747.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9419..11137
                     /locus_tag="PC479_001195"
     CDS             9419..11137
                     /locus_tag="PC479_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2803748.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11168..13168
                     /locus_tag="PC479_001196"
     CDS             11168..13168
                     /locus_tag="PC479_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2803749.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13519..13764
                     /locus_tag="PC479_001197"
     CDS             13519..13764
                     /locus_tag="PC479_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2803750.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13799..14524
                     /locus_tag="PC479_001198"
     CDS             13799..14524
                     /locus_tag="PC479_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2803751.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14546..15859
                     /locus_tag="PC479_001199"
     CDS             14546..15859
                     /locus_tag="PC479_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2803752.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16065..16604
                     /locus_tag="PC479_001200"
     CDS             16065..16604
                     /locus_tag="PC479_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2803753.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16693..18678
                     /locus_tag="PC479_001201"
     CDS             16693..18678
                     /locus_tag="PC479_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2803754.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18902..19870
                     /gene="spoIIIAA"
                     /locus_tag="PC479_001202"
     CDS             18902..19870
                     /gene="spoIIIAA"
                     /locus_tag="PC479_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2803755.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19860..20381
                     /locus_tag="PC479_001203"
     CDS             19860..20381
                     /locus_tag="PC479_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2803756.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20398..20595
                     /gene="spoIIIAC"
                     /locus_tag="PC479_001204"
     CDS             20398..20595
                     /gene="spoIIIAC"
                     /locus_tag="PC479_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2803757.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20611..20997
                     /gene="spoIIIAD"
                     /locus_tag="PC479_001205"
     CDS             20611..20997
                     /gene="spoIIIAD"
                     /locus_tag="PC479_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2803758.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21082..22287
                     /gene="spoIIIAE"
                     /locus_tag="PC479_001206"
     CDS             21082..22287
                     /gene="spoIIIAE"
                     /locus_tag="PC479_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2803759.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22347..22964
                     /locus_tag="PC479_001207"
     CDS             22347..22964
                     /locus_tag="PC479_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2803760.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22976..23530
                     /locus_tag="PC479_001208"
     CDS             22976..23530
                     /locus_tag="PC479_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2803761.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23557..24246
                     /locus_tag="PC479_001209"
     CDS             23557..24246
                     /locus_tag="PC479_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2803762.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24356..24718
                     /locus_tag="PC479_001210"
     CDS             24356..24718
                     /locus_tag="PC479_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2803763.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24803..25309
                     /gene="nusB"
                     /locus_tag="PC479_001211"
     CDS             24803..25309
                     /gene="nusB"
                     /locus_tag="PC479_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2803764.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25293..26363
                     /locus_tag="PC479_001212"
     CDS             25293..26363
                     /locus_tag="PC479_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2803765.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26360..27565
                     /locus_tag="PC479_001213"
     CDS             26360..27565
                     /locus_tag="PC479_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2803766.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27567..27782
                     /gene="xseB"
                     /locus_tag="PC479_001214"
     CDS             27567..27782
                     /gene="xseB"
                     /locus_tag="PC479_001214"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2803767.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27785..28672
                     /locus_tag="PC479_001215"
     CDS             27785..28672
                     /locus_tag="PC479_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2803768.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28702..29139
                     /locus_tag="PC479_001216"
     CDS             28702..29139
                     /locus_tag="PC479_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2803769.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29173..31038
                     /locus_tag="PC479_001217"
     CDS             29173..31038
                     /locus_tag="PC479_001217"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2803770.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31171..31983
                     /locus_tag="PC479_001218"
     CDS             31171..31983
                     /locus_tag="PC479_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2803771.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32003..33682
                     /gene="recN"
                     /locus_tag="PC479_001219"
     CDS             32003..33682
                     /gene="recN"
                     /locus_tag="PC479_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2803772.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33896..34762
                     /locus_tag="PC479_001220"
     CDS             33896..34762
                     /locus_tag="PC479_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803773.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34855..35346)
                     /locus_tag="PC479_001221"
     CDS             complement(34855..35346)
                     /locus_tag="PC479_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2803774.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35407..35847)
                     /locus_tag="PC479_001222"
     CDS             complement(35407..35847)
                     /locus_tag="PC479_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2803775.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36008..37066
                     /locus_tag="PC479_001223"
     CDS             36008..37066
                     /locus_tag="PC479_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2803776.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37208..38032
                     /gene="spo0A"
                     /locus_tag="PC479_001224"
     CDS             37208..38032
                     /gene="spo0A"
                     /locus_tag="PC479_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2803777.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38445..39566
                     /locus_tag="PC479_001225"
     CDS             38445..39566
                     /locus_tag="PC479_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803778.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39584..40459
                     /locus_tag="PC479_001226"
     CDS             39584..40459
                     /locus_tag="PC479_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2803779.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40483..41175
                     /locus_tag="PC479_001227"
     CDS             40483..41175
                     /locus_tag="PC479_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2803780.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41183..42007
                     /locus_tag="PC479_001228"
     CDS             41183..42007
                     /locus_tag="PC479_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2803781.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42012..43079
                     /locus_tag="PC479_001229"
     CDS             42012..43079
                     /locus_tag="PC479_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2803782.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43081..43617
                     /locus_tag="PC479_001230"
     CDS             43081..43617
                     /locus_tag="PC479_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2803783.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43685..44290
                     /locus_tag="PC479_001231"
     CDS             43685..44290
                     /locus_tag="PC479_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2803784.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44302..45216
                     /locus_tag="PC479_001232"
     CDS             44302..45216
                     /locus_tag="PC479_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2803785.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45258..46430
                     /locus_tag="PC479_001233"
     CDS             45258..46430
                     /locus_tag="PC479_001233"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2803786.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46477..47289
                     /locus_tag="PC479_001234"
     CDS             46477..47289
                     /locus_tag="PC479_001234"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2803787.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47493..48818
                     /locus_tag="PC479_001235"
     CDS             47493..48818
                     /locus_tag="PC479_001235"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2803788.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        19810 a         4930 c         9194 g        15046 t
ORIGIN      
        1 tatttagaca tttattacaa atcaaaattt attacatatt atttataaaa aaatctaagg
       61 aggaaataat aatgaaaata ttagtattaa actgtgggag ttcttcatta aaatatcaat
      121 taatagatat gaataatgaa gaagttttat gtataggatt ggttgaaaga ataggaatag
      181 aaggttctat attaaaacat gaaaaagcag gtagagatga taaatacgtt gttgaacaac
      241 caatgaagga ccacaaagat gcaatagcat tagttttaga agctgttgct catccagaat
      301 ttggtgcagt taaagaaatg aaagaaatag atgcagtagg acatagggtt gtacatgctg
      361 gagaaaaatt tgcaacttca gtagtaataa ctccagaagt agaagaagct ttaaaagaat
      421 gtatagattt agctccactt cataatccag caaacataat gggaatagat gcttgtaaag
      481 ctatacttcc agatgtacca atggtaggtg tatttgatac tgctttccat caaacaatgc
      541 ctaaatcttc atacttatat ggtttacctc atgagctata tactaaatat ggggtaagaa
      601 gatatggatt ccacggaact tctcataatt atgtatctca aagagcagca gagatattag
      661 gaaaagatat aaaagattta aaaatagtaa cttgtcactt aggaaatggt gcttctatag
      721 ctgctgttga tggtggaaaa tgtgtagata catctatggg attcactcca ttagaaggat
      781 taataatggg aactagatgt ggagatatag acccagctat attaccattc ttaatgagaa
      841 aagaaggttt agatgctgat ggattggata aattaatgaa taaagaatct ggagtatatg
      901 gaatgactgg aatttctagt gacttcagag atatagaaga tgcagctaaa aatggagacg
      961 aaagagctca agctacatta gaagcgtatg ttaaaaaagt acaaaaatat ataggcgctt
     1021 atgctgctga aatgaatgga ttggatgttg tagtatttac tgcaggcgtt ggtgaaaatg
     1081 gaaaggctat aagagcagat attgcttcta acatggagtt tttaggaatg aaattagata
     1141 aagaagctaa tgatgttaga ggaaaagaaa cagtaatatc tactgctgat tctaaagtaa
     1201 aaatgctttt aataccaact aatgaagaat taatgatagc tagagataca ttaagattgg
     1261 taaaataatt aaattaaacc tatgcctatc aagtaaaaat acttgacaaa caaatgctag
     1321 atttgtataa taaatttggt gatatgttga ggtgaactta atgaaagtta gcatagaaaa
     1381 aataaataga aaagaaactg acaaaataga cttgaatttt tgtgaaaaaa ttgatactat
     1441 tagttattgt gatgagattt acaaattagt atcacctgta aatttaaaag ggaaagtgtc
     1501 aaagactaat aaaggtttgt atctagatat agatgtcaat tttacaattg ttgacaattg
     1561 ttcaagatgt cttaaagaag tagaaatacc actagagtat tctatacaag gttttttagt
     1621 aaaagaagaa gattatgatg aagatgagtt tgaggaattt gatcctttta tattcgatgg
     1681 tgaagaaatc gaccttattg atataataga acagacatta gattttaatg tacctcataa
     1741 agttctttgt agtgaaaact gtaaaggtct ttgtcaagta tgcggggcaa acttaaatga
     1801 agaagagtgt tcttgcagtg aaattacaaa cgatgaggaa tacatagatc ctcgttttgc
     1861 taaattaaaa gatttattta attaaatttt gactaaggag gtggcgtaaa tggcagtacc
     1921 aaagcgtaaa acgtctaaat caaacacaaa aatgagaaga gcagctaact caaagatgga
     1981 agcaacagga tttgtaagtt gcccacaatg tcatgagcca aaattaccac atagagtgtg
     2041 tccagactgt ggatattata aaggtaaaga agttgtgtct aagtaatagc ttctaaaaag
     2101 catgtagagt atactctaca tgtttttttt gtacatataa aaaaataaga taatttacta
     2161 gagaaaataa gtggatttta tgcatattaa aaaatagaaa aataataata taagtattga
     2221 aaatttttgt gtgttactat atactactat tagtagctgg tactaattac agctataaaa
     2281 tttggaggga acatgaaaaa gaaaagtaaa gcccaaagac aaaaagagct tatagatatg
     2341 ctaaagacag atccttttta tactgatgaa gaattatcaa gtctgtttga tgtaagtata
     2401 cagaccataa gattagatag aatgagtcta aacataccag agctaagaga aagagtcaaa
     2461 tcaatagcag aaactcagag ttcaaaagta aagactttag gagtaaagga aattacagga
     2521 gaaatcattg atttatctgt agggcgatta ggtatatcta tgttagaagt tacacaggac
     2581 atgatttact ctaagactaa tactttaaaa gatacgtata ttttttctct agcagattca
     2641 ttggcaatgg ctataataga tgcacctaag gttattatga gagttgcaaa tgtaaaaagt
     2701 tttaagttga tagaacaaca agataggctt atagcaaaag cagaagtgta tagaaatata
     2761 gataaaaagc attatgtaaa agttgttatc aacaataaag cccaagagca aatatttaga
     2821 ggaaagttta tctttgaaga attagattag gagggaaatt atgaaaatag taattgatgg
     2881 aatgggtgga gataatgcac ctaaatctaa tgtagaaggc gcagtaaatg ctataaaaga
     2941 atatcaggtt gatttaataa tcacaggaga taaagattta cttgaaaagg aattttcaaa
     3001 ttatgaattt gatagaaata aattagagat agttcatact actgagatta tagaaaacga
     3061 agataaacca gtaaaggcta taagaagtaa aaaagattca tctatggtag tagcattaaa
     3121 tcttgtaaaa gaaggaaagg cagatgctat aatatcagct ggaaatacag gagccttact
     3181 tgctggtgga ttatttgttg ttggtagaat aaaaggaatc gatagacctt gcttatgttc
     3241 agcaatacca aatgtaaagc gaggaatgac acttatagca gactgtggcg caaatgcaga
     3301 ttgtaaacct aagaatctag ttgaatttgc agctatgagt aatatttatt caagaaaagt
     3361 tttaggtctt gaaaatccta aggtagcatt agcaaatgtt ggactagaag aaggcaaagg
     3421 caatgattta gtcaaaaggt catatgaaga aattaaaaaa ttagatttaa attttatagg
     3481 gaatgtagaa gctagagaag ttataaatgc atacacagat ataatcatat gtgatggatt
     3541 tacaggtaat atattactta aatctgctga aggtgtagca ctttcagtta tgagtcttat
     3601 aaaggaaact tttatggcca gtacaaaaag taaaatagga gcattactta taaaggatga
     3661 tttaagaaaa ttaaaaagtt ttatagatta ttctgagtat gggggagctc cacttttagg
     3721 tctaaatgga ggagttataa aagctcatgg tagttctgat gctaaagcta taaaaaatgc
     3781 tataaatcaa ggaataaaat tttcaaaggg aaaagtcgtt gaagatatta accaatttat
     3841 aagcaaatac aatgaagaaa ataaaaataa cgaagatgaa taaactcgtt ttggaggttt
     3901 aatatataat gaatacaaag gctggaatat taggagtagg aagttatcta cctgagcaat
     3961 catatgataa ttttcacttc gaaaaaataa tggatacatc agatgagtgg ataagtacta
     4021 gaactggtat taaggaaaga agatttgcca aagagtctga agcaacttct gacttagcat
     4081 ctaaagctgc cttaaaagct atagaatgtg caaagttaaa cgtagaggat atagagctga
     4141 ttatattagc tacaattact cctgatatgt ctttgccttc aactgcatgt atagtacaag
     4201 atgcaatagg agcagtaaat gctactgcat ttgatatatc agcagcttgt tctggatttg
     4261 tatatggagt aactattgca aagcaatttg tggaaacagg ttgctataaa aatgtgcttg
     4321 taataggagc agaaacttgt tctaagtttt taaattatga tgatagaaca acagcagtat
     4381 tgtttggaga tggagctgga gcagctgtta ttggccctgt aaatgagggt ggaatactgt
     4441 ctacacatat gggctctgat ggtaaaggaa aagattgttt aaaagtacct gctggaggtt
     4501 caagacttaa ggcaagtaaa gagactgtag aagctaatct acatacaata gaaatggcag
     4561 gtagtgatgt gtttaaattt gcagttagaa agatggcaga aacttcttta agagctcttg
     4621 aaaaagcaaa tctaaataca actgatatag actacttagt gccacatcaa gctaatataa
     4681 gaatcataca agcttcttct aagagattag agcttgatat gaaaaaagtt tatgtaaata
     4741 tagacaaata tggaaatatg tctgctgcat ctataccagt agcattagat gaagcttata
     4801 gagaaggtaa aattaaaaag ggtgataatg tagttttagt tggctttggt ggaggtctaa
     4861 cttggggggc ttcagttgtc aagtggactt tatagtataa aatatctaaa atgatttaat
     4921 taacaaaatt aaacttatac acaaaataaa gttgattaat aataaaaagt ttacttttat
     4981 gatttcaagt gaaggagaat tgtatgaata aaatttgcaa aatattaaat ataaaatacc
     5041 cagttatcca agggggaatg gcatgggtag ctactgcatc attagcaagt gctgtatcta
     5101 atgcaggagg acttggcata atagcagcag gaaatgcacc aaaagaagct ataaagaaag
     5161 aaattgttga gtgtaaaaaa ttaacagata agccttttgg agtaaatgta atgcttatgt
     5221 cgccatttgt tgatgatata attgatttga ttatagaaga aaaagttcaa gttattacta
     5281 ctggtgctgg aaatcctgca aagtatatgg atagattaaa ggaagctgga acaaaggtta
     5341 ttcctgtagt acctacaata gctttggcac aaagaatgga aaagctagga gctacagcag
     5401 taatagcaga aggtactgaa ggtggaggac atataggaga acttactact atggtcttag
     5461 ttccacaagt tgctgatgct gtaaacatac ctgtaatagc tgctggagga attgtagatg
     5521 gtagaggaat tgcagcatca tttgcattag gtgccagtgc agttcaagta ggaactagat
     5581 ttatttgcag tgaagagtgt tctgtccatt caaactataa aaacttagta ctaaaagcaa
     5641 aagatagaga tgcaattgta acaggaagaa gtactggtca tccagtaaga acattaaaaa
     5701 ataaactatc aaaagaattt ttaaagatgg aacaaaatgg agctactcct gaagaactgg
     5761 ataaaaaagg tacaggagct ttaagatttg caacagtaga tggagacata gaaaaaggtt
     5821 catttatggc aggtcaaagt gctgctatgg taaaagaaat aacaccttgt aaggaaatta
     5881 tagaggctat ggtaaatcaa gcaagagaga ttatgccagc aatagaactg taaatcagga
     5941 ggagcaagat gggaaaagtt gcactagtat tccctggtca aggagctcaa tatgtaggta
     6001 tggccaagga tttatatgaa aataatgctg ttgctaaaag tgtaatagat gaggctagtg
     6061 atgccttaaa tatggattta aaaaacctta tgtttaatgg aaatgaagaa gaactttcta
     6121 aaactgaaaa cactcaacct gctatggtta cacatagtgt agcagtatta aaagcggtac
     6181 aagctcaaat agatttaaaa tataatgcat gcttaggatt atccttagga gagtatagtg
     6241 cgttggttgc agcagatgca attgacttta aagatgctgt atgtttagtt aaaaaaagag
     6301 gtaagtttat gcaggaaaca gttccagcag gtgttggagc tatggcagca gttttaggtt
     6361 tagatagaaa tgtacttgaa aatgtggtta aagatataca aggaggtatt gtagaagtag
     6421 caaattacaa ttctcctgga caaatagtta tatctggtga aaatgaaaag atagaagaag
     6481 caatggtaaa atgcaaagaa gcaggagcaa aaagggcagt taaattaaat gtaagtggac
     6541 cttttcactc atcaatgtta aaagaagctg gtgtaaaatt ggccagtgaa ttagaaaaag
     6601 ttaacataac taaacctaaa gttgatgtgg tggcaaatgt aaatgcagat tactataaaa
     6661 acgaaggtaa agacctgctt ataaaacaag taagttcatc tgttctttgg gaagattcta
     6721 tagaaagact tttaaatgat ggatatgata cttttataga aatgggacct ggaaaaactt
     6781 taaaagcatt tattaagaaa atagcttcca ataaaaaagc aaatgtcaat atttataata
     6841 tagatggtat agattctctt aatgaatttg tacaaaatta tagaaatgga gagatataat
     6901 gataaatctt acaggacaag tagcagttgt tactggtggt tctaggggta taggaaaaga
     6961 aatagcaaaa aaactagcat cttttggagc tgatgtagta atcaattata cttctaaaga
     7021 agatgaagca ctaaaaacta aaaatgaaat agaaagtatg ggggtaaagt gtacctctat
     7081 aaaatgtgat gtgtctaaat ttgatgaagt gaatcaaatg atagattctg ttgtaagcga
     7141 atttggaaaa attgatatat tggttaataa tgcaggcata actaaagatg gtctgcttat
     7201 gagaatgaaa gaagaagatt ttgatagagt tatagatata aacttaaaag gtgtctttaa
     7261 ttgtacaaaa gcagttacta aacctatgat gaaaaagaag tatggaagaa taataaatat
     7321 gacttcagta gttggaatta tgggtaatgc agggcaaact aattattgtg catcaaaagc
     7381 aggtgtaatt ggatttacaa aagcttctgc aagagagtta gcatcaagaa acataaatat
     7441 aaatgcagta gcacctggat ttatagaaac agatatgact aaagtactaa gtgatgatgt
     7501 aaaagaatca acactagcaa acataccaaa gaaatcttat ggtaaaccag aagatgtagc
     7561 caatgccgta gcatttttag ttagtgacat gtcaagttat ataacaggac aagtaataaa
     7621 tgtagatggt ggaatggtaa tgcaataata atttaaaaag tagagaaatc tattaaaata
     7681 aaaaatttta ggaggtacac aatgtttaat aaagttgtag aaataataat agagcaatta
     7741 ggagtagaag ataaagaaat aactatggaa acatcattaa tgaaggattt agaagcagat
     7801 tcattagatg cagtagaaat aataatggca cttgaagatg aatttggtat agaaatacca
     7861 gatacagaag cagaaaactt caaatctata ggagatatag ttaagtatat agaagctaat
     7921 aaatagtcga gctttaaaaa agtggaggta tccgatgaat aaaagagtag taataacagg
     7981 acttggatgt gttacgccgt tgggaacagg aaaagaagag ttctggagta atataaaatc
     8041 tggagtatct ggtatagata aaataactaa ttttgatgct agcacatatc aaactcagat
     8101 agcaggagaa gttaagaatt ttcatccaga agaatatata agtaaaaagg aattgaagag
     8161 attagataaa tttgctcaat ttgcaatagt atctgctaaa ttagcagttg aagatgcaaa
     8221 tcttgattta gataaagttg atagagaacg ttttggtgtt ataataggtt ctggaatagg
     8281 tggagttgaa gctatagaaa ctcaacataa aattcttttg gaaaaaggaa ataaaagagt
     8341 aagttcactt tttgtaccta tgatgatagg aaatatggca gctggtcaag tttcaatatt
     8401 tttaggagca aaaggaccta acactaatgt atgtacagct tgtgcatcag gaactcattc
     8461 aataggtgat gcttttaagg taattcaaag aggtgatgca gaccttatgg tagcaggggg
     8521 ttctgaagct gctgtaacag gtcttgcttt tgctggattc tgtaacatga aggctatgtc
     8581 aactagaaat gatgacccta aaactgcatc aagaccattt gataaagaca gagatggttt
     8641 tgtaatggga gaaggagcag gtattgttat cttagaagac ttagaacatg ctttagcaag
     8701 aggtgctaaa atatatgcag aagtagttgg atatggttta actgctgatg cataccatat
     8761 gacaactcca gctgaaaatg gagagggagc agctaggtct atgaatatgg ctttgaaaga
     8821 tggaaatgtt ccattagaag aagtagatta tataaatgct catggaactt caacttatta
     8881 caatgattta tatgaaacta tggctataaa aactgtattt ggagaaaagg catatgactt
     8941 atgtgtgtca tcaacaaaat ctatgactgg tcacttatta ggagcatcag gtgctataga
     9001 ggctgttgtt tgtgctatga gtatagaaga tagttttgtg cctccaacta taaacataca
     9061 agaagttggt gaagatttgg atttagatta tgttccaaat caaggtaaag aaaaaaatat
     9121 tagatatgca ctatcaaatt ctttaggatt tggtgggcat aatgctacaa tagtactaaa
     9181 aaaatatgta taaataaata ttaaatataa aactaaaaaa caagcacttg ttttaactgt
     9241 ataaatttat attagcttaa atttaaagtc tcctaaatta ttttaggaga tttttttatt
     9301 aagatttaag tctaggatat gttgtaaatc attttgatat attgtattat taattattaa
     9361 gaggttattt atatatgcaa gttttcattc tacaatatac aaaaggagga ctaaaatttt
     9421 gaattttaga ctaccaaaga aaaggttacc caaaaagata atttttatat gtataatact
     9481 tattagtatt attttgttta taaagtatga ccaaaatgga ttgaaagaaa gagaaattat
     9541 aaataaggga aaatatatgt ctgaaagcct tgtggcagat aaggagtatg atgaagcaaa
     9601 aaaagtggtt aagacttctt ttgaatctat tccagaaaaa aaatacaata aagtatataa
     9661 tgaagtttgg gatatgttaa agactatttc atatgttcca gatggaacga agatagtcat
     9721 tgattcttta gaaaagatga gagaatcaga tgtgaaatta tctgatgaat gtagatttaa
     9781 tatagaaaaa agattagcat cagtgtatat aatggataat aattattcta aggcagtaga
     9841 tttaactgta aagtctataa agttggctga gaaattgggg aataattatg aaaaagcaag
     9901 actagatgtt gatttggcga gcatattttt aaagataggt ggctatgaaa caggagagaa
     9961 attaataaag gattctttta aaatagatat tgatgaaggt gaaaaaaatg gaatggttag
    10021 attatatgct attataaatc tagcagaaat atatgtagaa gagggcgaat atgataaagc
    10081 tattgaaata agtagcagga taaaagatta taaaaagttt gtaaatacgg ataattacaa
    10141 tgattttgat ataatggcct ctataatgca gtcaaatgcg tatgtgggca aaaataatat
    10201 acaaaaggca aagacttttt tagaaaaagc tgattatctt ataaaatcag atagaacagt
    10261 ttatatatta gataaagata tgtactatta tatggcaatg ggaaacttag aatataaaag
    10321 cgaaaattat aattctgcaa tagataatta taaaaaaagt ttagagatat ctactaaaag
    10381 aaagttaacc caggagaaaa taaaaaattt aaataagata ttaaatatat atgaaaaaca
    10441 aggaaatata gaatctttaa atgagtatca aaagatgtta ataaatgaat ataaagagaa
    10501 caaaagtata agagattcag agactagttt ctatataatt gataaagtat caaacgaaag
    10561 taagctattt gaaaaaacta aaaaagaaat aaaatattat aaaatattat ttacagtaat
    10621 tttaattgct atattagggt ctactttttt atacaaccga ttgaagtatt ttaaagcaca
    10681 aaatttacat gatggtctta caaatgttta taatagaaaa agttttgata ttatgtatga
    10741 aaagtacaga gagaaagatg ataattttgc acttgtgatg atagatattg ataatttcaa
    10801 gctcataaat gataattatg gacataaatt tggtgacact gttataaagg gaataacaaa
    10861 taccatttgt gttatgttag aacaagatga taaagtcttt aggtacggag gagaagaatt
    10921 ttctgtatta ataagaaata aatctggtga tgaagtagca gatataattg acagtataag
    10981 ggttgcaatt gcaaataaaa agtggaatga tgatgtgact gtgactataa gtgcaggtgt
    11041 agctcatgtt tcagatagca aagatgtact agaagaagct gataaaaatt tatataaagc
    11101 aaaacaactt ggtagaaata aagtagtata taagtaatta tatactatat aaataagagg
    11161 tataaatatg aaacaatcaa tagctatagt aactgataaa ataactaaac tatcatcttt
    11221 cttagaagaa aatatacgtt tggttttagg taattatatt gatataaacc actattatct
    11281 ggaagtttta aaagagaatg ataaaataca aggtgatttt gtactagtaa tgaatgatga
    11341 tagattaaac agtatgagaa agcatttagt acaagatagt aagattatag tgataaggag
    11401 aacacttaaa gaaaatgaaa tatatggatt attttcaata ccaaatggaa ctgaagtatt
    11461 ggttgtaaat gatacaaaag aaactacctt agaaacgata agcttatttt acaaaatagg
    11521 agtaaaaaat ttaaagttta ctccatatat tgaaggaaaa atttataaaa atatagacat
    11581 agctataaca cctggagtaa agggaaaagt accaagttat ataaataaag taatcgacct
    11641 tggaaataga tatattgata tatctacctt tatagaaata ataaatggtc ttagaataga
    11701 tgttaaagaa atacgtaaaa atttaatggc atattctgag agtttaataa gtttagataa
    11761 tggagttaaa gataattata ggcaactgtt tttaaaaata gaagaacaag atgttatatt
    11821 gaatctatca aaagatggaa taatatttac ttcagcagat ggtataataa acacatttaa
    11881 taaagaagct ttaaaaattt taggtataaa tgaaaatcca caaggcaaaa acatagaaga
    11941 tatcatttct gaagatttaa atattttact tgaagacaaa gaaataattg atgaagtggt
    12001 atttacaaat aaaaaatata taaatgtaaa taaaaaaagt attttttcta tggggaataa
    12061 agctgggata tattataact tacaagaaat aacgtatata aaaaaattag aacaaaatct
    12121 tactaataaa ttaagagaac aaggtcagat agcaagatat acatttaaag atataaaaac
    12181 taaaaataaa aatatggtaa agtgtgtaga attggctaaa aaaatatcta aatcagacct
    12241 ttctgtatta ataataggag aaagtggtac tggtaaagaa ttattatcac aatcaattca
    12301 taatgcttcc aatagaagta atcaaccgtt tatagcagtt aattgtgcag cagttccaga
    12361 tagtctatta gaaagtcaac tgtttggata tgaaaaaggc tcttttacag gagctttaag
    12421 agaaggaaaa aagggtctat ttgaacttgc taataatgga actatatttc tggatgaaat
    12481 tggagatatg cctccattgc tacagacaaa gttactaagg gtattacaag aaaagcaggt
    12541 tatgcctata ggttcccata atgttataaa catagatgtt agggtcatag cagcaacaaa
    12601 caaaaactta ctaaagatga ttagagaagg aaaatttaga gaggacctgt attatagact
    12661 aaatgtattg ccaatagatg taccatcatt gaggaataga aaagaagata taataacact
    12721 tatgaatttc tttttgaaaa acaatataag actttcgcca gaagttataa ctattttaga
    12781 aaaatataat tggccaggaa atataagaga gttacaaaat gttgcttctt atgtaagtct
    12841 aatgtgtgat agtatggttt ttagagatga cttaccacca tatataaggg tagaatgtgc
    12901 taacaattat gaagatggat ttggttatac aagaaaagaa tttgaggaaa tactgaggat
    12961 tctatatgaa agaagagagt catcctctgg aattggaaga gggtttataa tcgaaaagct
    13021 tatacaggaa ggtattgaga tttctgaaag caaagttaga aaggtgctat cttatttaag
    13081 tgaatgtgga tacatatcat ctaaatctgg tagaggagga agtcaaataa cctcagaggg
    13141 gataaaactt tataataata taaattaagc taagtatttg atattgaaat cacatatttt
    13201 gaatttagat atttaaataa taataaactt ttatatttat agagattaat ttatattatg
    13261 tcagaaagaa tattgtaatt tataattaaa tattaatagg tttttaaaat aggattagat
    13321 aggtaatagg ttaagttaga ataacccatt acctattttt atttgtaaga aaagtgtaca
    13381 tagaattaga agtaaaaaat acaactaata aaactattga aaatactaga taaataataa
    13441 ataaaaataa ttatagattt tggcatacat attgctaatt atagagataa gacgacaaaa
    13501 taattgggag gtttaatgat gaaatacaaa acaacaggga cttgtgctac agaaattgaa
    13561 tttgaagtaa aagaaaataa agttacaaat gtaaacttta taggtggatg tgatggaaat
    13621 ttaaaagggc taaaagtatt agttgaaggc atgaatatag aagatgtaat acaaaagtta
    13681 aaaggaatag agtgtaaaac aaagccaaca tcttgcccag accaactgtc tctagcattg
    13741 gaaaattata tgaatataaa gtaatacata aaacaatcga ttagttagga gtttaattat
    13801 gaaaccaata atagggattt taggaaattt aataataatg gaaaatggta tgtttccagg
    13861 tcttgaaagg tcatatgtaa ataatgatta tataaatgca gtgctaaaag gtggcggtag
    13921 tccagtaata attcctgtaa atacagataa agaagttata aaaaaacaga ttgaaatggt
    13981 tgatggagtc ttaatatctg ggggatggga tatcaatcct caactgtatg gagaagaaac
    14041 aagagaagaa acgacattta tatatccaga agtggatgaa tttgatttaa ttgcaataag
    14101 tatagcatta gaacttaaaa agcctatatt aggtgtatgt aggggactac aaattttaaa
    14161 tgtaagttta ggaggaactt tatatcaaga taataatctt atagaaggaa gttatataaa
    14221 acatacacaa tcttctaaaa gacatgtagc tactcataaa gttgatgtta aagaaggaag
    14281 tattttagag ggaattttag gaaaacaatt acttacaaat agttatcatc atcaaagtgt
    14341 caatcagctt ggaaaaggat taaaagccat agcatactca aaagatggta taatagaagc
    14401 tattgagaaa gaggatgaaa attttgtagt aggagtacag tggcatccag aaatgatggt
    14461 agattactgt gataaaatgg aaaaactatt taaatatttt ataaatattt gttcgaattt
    14521 ataatatacc ttggggggat tgaacatgaa cgaaaagaac aaaatgggat taataagtat
    14581 tatactttta ggtattaatg cagtagtagg agctggagtt tttctattac caggagatgc
    14641 aatgaaatca tttggtgtag caagtatatt tgtatatata tttgatatgt tgttagtttt
    14701 atctatggca ttctgttttg ctgaggttgc aggtaaattc aataaaaatg gtgctgccta
    14761 tgtatataca aaagaagctt ttggagattt ttgtggtttt gaagtaggtc ttatgaaatg
    14821 ggtaatagga tgtatttctt ggggagcttt aatagtaggt tttcctacat cattatcagc
    14881 agtatgggct ccagcaggtg aaccacatat acaaaaaatt ataattgtag ctatgatagt
    14941 tgggcttaca attataaact tattaggtgt atctctatca aaaattgtac aaaatgttat
    15001 aactgtaggt aaactaattc cattaatact atttatagga ataggtatat tttttataaa
    15061 aggagttaat tttacttctt caacaatggt acctccagga gctggagcaa cagagtttgg
    15121 agcagcagca cttttaatgt tttattcatt tacaggattt gaatctatag cagttgcagc
    15181 agaagatatg gaaaatccac aaaaaaatat accaatagca attataagtg taatagtaat
    15241 tgcatcaata atatatatat taaatcaagt tgtatgtgtt ggtatactag gagattcgtt
    15301 atctagtaca tctacaccag tggcagatgc tgcaagaata tgctttggaa atatgggagc
    15361 tggattggtt acctttggaa cattggtttc tgttggtgga atttgtatgt gtggtgcttt
    15421 tgtaaatcca agaagttgtg tagctttagc tgatgataaa atgttaccga gaatatttgc
    15481 aagaaaagat aaaaaaggta caccatatgt agcgataata gctactatgt taatcactat
    15541 accaatagct ctatctggaa gttttgctga attagcagca ataagtgcag ttgctagatt
    15601 catacaatac ataccaacat ctttatccgt attagtgttt agaaagaaaa gaccagaatt
    15661 agtaggtact tttaaaactc cttttggagc agtgattcct ctaattgctg tatgtgttgg
    15721 aacatggttg ttattccaag catctatgca tcaattaatt atgggattag gagctttggc
    15781 aattggagta ccattatact ttataatgaa gtcttataat agaaaaatct atggtgaaaa
    15841 tttaaagaaa atagtttaat tatgttataa caatttattg agttaaaatt agacattggc
    15901 agaaaattaa atacaaataa gtatctacaa ggctatatta aaatattatt aattttttat
    15961 tttaatatag ccttatattg ttatcaataa atgaaaaata ttgtaacaac agaagaacaa
    16021 tagtattatg tttatagaca aaaaacttat taggaggtaa atttatgcca acaataacat
    16081 taaaaaatgg tgtagacgta cttattagag aaggtgtaag agaagatgct caaagcatta
    16141 tagattttta taatgaagtg ggtggagaaa ctcattttct gtcatttgga aaggatgaat
    16201 ataaaatatc tttggaggaa caagaaaatg ctatagaatc agcaaaagca tctgataatt
    16261 ctgttaaact gattgcattt atagatggtg aaatagttgg gattgcaacg attgattcta
    16321 atcaaaaagc aaaaggaaag catgttggag tattaggaat tgttgtaaaa gaaaaatatt
    16381 ggggaattgg attgggtaaa aggcttatgt tagaccttat agaatggtgt aaatcaaatg
    16441 gaataaccaa gaagataact cttgtcacta atgaagaaaa ttataatgct ataggattat
    16501 acaaaaaagt tggatttgaa gtagaaagca tactaaagaa agaatgttat tataatggtg
    16561 tttatactga tttaatagga atgtcacttt tattgggaat ataattagtg tacattttat
    16621 ctcgaaaaaa gtatattaag tatggtataa ttaaaaaaga atataactta atataatttt
    16681 ggaggacgaa ttatgaaaaa tctgtgtaaa atatcagatg attatttaaa cagcaagctt
    16741 aagtatttaa agttattatc aaaacaatat ccaagtatat caaaagcaag tacagaaata
    16801 ataaatttag aggctatatt aaatcttcca aagggaacag agcattttat aacagatgta
    16861 catggtgaat atgaaccatt tgtacatgta ttaaaaaatg gttctggtgt aataaagaga
    16921 aaaatagaag aattgttttc aaatacaata agagacagcg aaaaaaaaat gttagcaact
    16981 cttgtatatt atcctgaaca aaagttagac ctaataatta agcaagagga aaatatagat
    17041 gatttttata gaataaatat ttatagactt atagaacttt gtaaatatgc ttctagcaaa
    17101 tatactaggt ctaaagttag aaaactgtta ccagaaaatt ttaaatacat aatagaagaa
    17161 cttttgcatg agcatgttaa aagtgaacat aaagaagagt attataaaag tatagttgaa
    17221 acaatagttg acataggaat agctaaggag tttataattg ctatttctac agttatacaa
    17281 aaattggttg tagatagact tcatgtaata ggagatattt atgatagagg tccaagacca
    17341 gatataattg tcgataagct tattgaacat cattgtgtag atattcaatg gggaaatcat
    17401 gatatattgt ggatgggtgc agcctcaggt gaaaaaactt gtatagcaaa tgcactgagg
    17461 atatcagcta gatatgcgaa tttagacatt gtagaagaca tatatggaat aaatctattg
    17521 cctttagcca cttttgctat agaaatgtat aaagatgacc cttgtaaaga atttattcca
    17581 aaggttaatg accaaagtgt aacaacaaca gaaaagtctt taatggcaaa aatgcacaaa
    17641 gcaataagta taattcaatt taagcttgaa ggtgaggtta tcagacgaag acctgaattt
    17701 gaaatggaac atagactctt gcttaatatg ataaactatg atgaaggtac gattaattta
    17761 aaaggtaaaa catataaatt aaaagatact tatttaccta caatagacaa aaaagaccca
    17821 tataaattaa ctatggaaga aagaaatgtt attgataaat tagtatcttc atttagaggt
    17881 agtgaaaaac tacaaaaaca tgtttcattt ttgttttcta aaggaagtat atatttaaag
    17941 gctaactcaa atttactgat tcatggatgt gtaccgttaa atgaagatgg aagctttatg
    18001 tctatgaata taatgggaaa agagtataaa ggtaaggctc ttatggataa gatggagtct
    18061 ctagcaagag aaggattttt ctttaaagat aaggcagaag aaaaactgta tggcatggat
    18121 attatgtggt atttatggac tgggaaatgt tcatcattat ttggtaaaga tgatatgact
    18181 acatttgaaa gatactttat agctgaaaaa gagactcata aagaaaataa gaacccatat
    18241 tttaaactta gagaaaatga gatggcttgt aaaagattat ttgaagaatt tgatttagaa
    18301 cttgatgaat ctcatataat aaatggtcat gttcctgtgg agagtaaaaa tggagagagc
    18361 ccaataaaag ctaatggtaa aattctcgtt atagatggtg gtttttctag ggcttaccaa
    18421 aaaactactg gtatagctgg atacactttg atatacaatt ctagaacttt gcaattagta
    18481 tcgcatgaac catttaattc tgctgaagaa gccattgcaa atgaaagtga tatcttatcg
    18541 acaactgttg tagtagagca taaggcaaag agaaaaatgg tgagagatac tgatgaagga
    18601 ataaagatac aagaggaaat agaagattta aaactacttt taatggctta taagaaaggg
    18661 ctaataaaag aaatgtagat gttatagagg cacttcatga ttgaagtgtc tttttgttaa
    18721 tacttattaa atatattata aagattaata atagtagttt atattatact tactaagtga
    18781 atccaagata aatgggttca ctttttttat ttataaacat tattaaaaaa atatagatgt
    18841 tttggttata ttccttgtcc tttataaata gaattatata aacaaaggag gaggacaaga
    18901 gatgaataaa ctttctgatg aaataataaa ctctctttct acaactataa gagaaaaggt
    18961 agaaaaagtc tcaaacaata atctgaacat agaagaaatt aggttacgct cacaaaaacc
    19021 attgatacta aatgcaaact ctaaagatta tttttacaat caaaaaacta tgacacttga
    19081 tttaaatcaa caaaactcat atgtagtaac aagagaagat gtagagcaaa cttttcaaat
    19141 aatatgtaaa tattcaatac attcatttat ggatgacata aaaaaaggat tcataacttt
    19201 gagaggaggt catagagtag ggttagttgg aaaagcaata gtagaagatg gacaagtcaa
    19261 aaatataaag catatatcat ctttaaatat aagggtatca agggaaatta ttggatgctc
    19321 ggataagatt ttaagtcata tcataaaggg aaaaaatcag attaataata ctttgataat
    19381 atcaccacct caatgtggaa aaacaacttt aataagagat atagtaagaa atttaagtaa
    19441 tggtaatgaa gattatggat ttaaaggttt aaaagtggct ttagtagatg agcgtaatga
    19501 gatagcagga gcttatctag gtgttccaca aatggatgtg ggaataagga ctgatataat
    19561 agaaacttgc ccaaaagact tgggtattac aatgctttta aggtctatgt caccaaatgt
    19621 tattgtgaca gatgaaattg gaagtgaaaa agaaataaag gcattataca cagcattaaa
    19681 tggaggtata ggattaataa ctactgtgca tggagattca atagaggata tacaaaatag
    19741 aaaagaatta aatagacttc tggataaaga gttatttaaa aaggttataa ttctttctgc
    19801 aaaaagagga gcaggaacaa ttgaaaaaat ttatgattta gaagaaaaga gatggtattt
    19861 tgcaaattaa aataattatt atagctttct tgataggaag tagttatttg ataggtgaac
    19921 aaatatacaa aacttatact cgtagacata aacaattaaa cgatttaata agagttttag
    19981 aaattttgag gatggattta tcttttggac tttatacatt agaagaaatc tttaatagga
    20041 taggtggaaa taaagagttt tgtttttgga aatttttcta tcaaatatca gaaggacttc
    20101 acaatgaaca aagtaaaact ttggaaataa taatatcaga aaatattgat gtcttaagta
    20161 aagaaactta tctaggcaat aaagaaattg aggaactgaa aaatctaata cttactttag
    20221 ggaaaagcga tatagagtca caacaaagaa tgatagattt atctatagaa aatttgaaaa
    20281 aacaaaccta tgagacaaag gaagatataa acaaaaaagg cgtattgtat aaaaaattag
    20341 tgacattcat aggtataggt atatgtatta tattgattta atatggggag gtttattatg
    20401 gaaatatcat tgatattaaa ggttgcaggt gtcggtattc taatatcagt attgaatatg
    20461 atattggaaa aaacagatag aaaagattgg gcaggtctta ctacattagc aggtgtaata
    20521 atcgtattgg gtatggtaat aacagaaata agtgacttgt ttaatactgt aagaacaatg
    20581 tttcaacttt actaaagagg agggagtaat ttggaaataa tgcaattaat aggaattgca
    20641 atcatttcaa ctacactctg tttagtaatt aaaaaagata gaccagagat agcaaatttc
    20701 atagctataa taactggagt aataatacta ttgtcagtta tgtttaagct aaattttata
    20761 gtagatagta tacaagattt ggctaacaag gctaacatcc caactatgta tatatcatta
    20821 ataataaaat taattgggat agcatactta atggaatttg cgatacaact ttgtaaagat
    20881 tgtggggaag gaaacattgc ttcaaaacta gaatttggag gtaaaataat agttatgtca
    20941 atgtctttcc ctatactttt gtccattgta gaaatggtgg tgaatataat tccatagatg
    21001 tgtatcttgg aatgaatata agtatattag agagctttcg ttgtattgag gtttatagag
    21061 tgaagagagg catggttgct tatgaaaaaa agatttttac caatgattat aggtttttta
    21121 tttactttct tttttgtaaa tatctttgct attatgatat ttgctaatga agtaccaagt
    21181 agtgaagata aagaatatgg tgaaacaaaa aatagcatag ataagtatat agatggtcaa
    21241 ttagacaagc tagatataaa tgagattcaa gactatataa ataaagaaat agttattaat
    21301 gatgttaatt taaaatcatt tgtaaaagat ttaattagtg gagaaaaaaa tatcttagat
    21361 ttattcaata aagatggatt gaagatattg atgtttgatg aatttaaagc aagtttaaag
    21421 gtagtagcag taattttggt actggctctg ttatcatcta ttttaaagag tctggaaaac
    21481 tctttttcat caggtgcagt aagtcaaata gcaacatata taatatttat cacaatggta
    21541 tcacttacac ttattggttt taaagatgta ttacagattt gctatgatgc aatagaccat
    21601 actgtaggtc ttatgcaagt tataatgcca atattaataa catttttact tttaattgga
    21661 ttcccaataa cgtcgactac tttgaatcct atcttcatag gaggagtaac atttataaat
    21721 gtatttttta aaaattttct atttgtatct attactgttg catttggaat tttaataata
    21781 aacaatttat caaaaaatat aaggctaaag agatttttct cctttgtaaa acaaataaat
    21841 tatgtttcta ttggagctat gtttactgtt tacttaggtc ttgtatccat acaaggattg
    21901 tatgtaacca gttttgataa attcagtgta aagactgcta aatttgctat aggtaatttt
    21961 attcctgttg taggtggatt tgtatctgac tctgtggata ttttattatc ttcatctcag
    22021 cttataaaaa atatatttgg aggaatagga cttatcttac ttgttggaat ttgcttactt
    22081 ccagtaataa aaattttatc agtaatagta gtgtacaaat tagcagcaat aattgtagag
    22141 cctgttggag aggatggaat atctaatttt ttaaatgaag tggcaaattt aatgataata
    22201 atgctagcat cagtaattgc tataacagta atgttctttg taactgtagc tatcttgacc
    22261 tcgataagtg tcgtaagtca agggtaattt atatatcagt gaaaaataat tacatttaat
    22321 ctaatgtttt tagagaggag gatgctatgt tagagggcat aaaggcatgg atagttagtg
    22381 ttttgatagg agcatttata gtaaatatag tagatatgat tttaccaagt tctaagataa
    22441 aaccttatgt caacctagtt ttaaacttta tgtttgtatt tattgtaata actccagtag
    22501 taggattttt ttcaaaagat atgagtttag aagatagaat attaaaatct atgggaaact
    22561 acaacaagca gtatgtagat agtacaaatg ctttggcaaa agagactgga aataatagtc
    22621 tatccaaggg atatgaggat gggttaaaag aagtacttaa attaaaactt gatgaatatg
    22681 gttatgattt agaagatata gagcttaatg gggcaaatat aaataatata aaaataaaag
    22741 aaaagaataa tagtgcaaaa agtaataaca gttccaatga agaaaataaa aataatagta
    22801 ctaaaagaga ggaagaaaac ataaactcta atgacaaaga aaattctaaa caagtcttca
    22861 aaaaaggtac agagtatggg ttgaacctaa atgaagaaaa attaaaaaac gacttaataa
    22921 aggtgctgga tgtttctata gaagatatac aaattgataa ataggagttg ggagaatgtt
    22981 gaaaaactta aatgacaaag ataagagaaa ggtatattca ctaataacta tagcaggaat
    23041 atgtgtggtt tcattagtat tattatcttg ttttcctagt agcaaaagcg ataaacaagt
    23101 tggaaaaaca gagactaata aaaatgctga aaagcaagtc actaaagaac aagaaaaaga
    23161 tgatttagag tcaaaattaa cagcaatact atctaaaatt gatggtgcag gagacgttga
    23221 tgtaatggtt acatttgaat caagtgaaga aatacaacct gcttttaatt caaataatac
    23281 aacagaaaca acagaggaaa aagatgcaca aggtggagaa agaacagtta cgacttctag
    23341 tgaaaataaa acaatgataa cttcaaattc aagtgaccct gtagttataa aaactacaga
    23401 gccaaagata aagggagtga tagtggtagc aagtggagca agtgacccaa gtgttaaaga
    23461 gacactttat agtgcagtac aaacatcatt acaagtagct ggtcatcaag tagaaatata
    23521 ttctaaataa atacatataa ccggggggat aaatgaatga agtttaatta taagggaaga
    23581 ggatttgtaa taataacttt aactgcaatg ttagtggtag taggaacagt gaattatcaa
    23641 ctaagtaaga aatctttatt agaaacatca aaagagttta aggcatatga agaagcacag
    23701 ttgcaaaaaa atactgatga gagtgatagt agtaagacag aggattctag taatgtagat
    23761 aaacaagatg gtaaggaaag tgctgatatt gatatagttg atagcaaagc tagtaaagta
    23821 aaagaaaagg caactgagac aagtaaggaa ataaaagctc aattatcatc agagaaaaat
    23881 atgaaaaagg catcttatat tttagatatg aaaatgaata gagaaaaaca aagaaatgag
    23941 ttagttcaag atttaaatga aatgataaat aatccatcta caacagaaga atctagaaaa
    24001 gaagcttcta atatgaaatt aaatatagtt aaaattcaag aaaaagaact tcaaatagaa
    24061 aaccttttga gtacaaaagg gtatgaagaa gcgttagttt atataagcga taacaaagtt
    24121 aatgtagttg taaatgaagc taaattagaa aagaaagatg cagcaaaaat atttgattta
    24181 gttgcagaac aagccaatgt gaagtatgaa aatattaaac ttacaaataa taatagtaat
    24241 aagtaaagta taaacctcag aattaactga ggttattttc ttgcaaagta gacttgtatt
    24301 tggtattatt atacttataa aaatagttcc ttaaaatcag gaggtaagtt ttaatatgga
    24361 agataataaa tttggacaag taaaaatatc taatgatgta atagctacaa tagctggttt
    24421 agcagcatta gaagttgaag gcatagaaac tacagcaaca ttaacagata agttgttaaa
    24481 aaataatgga gtaaaaatac aaatcgaaga agaggatgtt aatttagatg taatggttac
    24541 gataaagtat ggaatgtcaa taccagatac agcttttaaa gttcaagaaa atgtaaaaaa
    24601 tactgttgag acaatgacag gactgaaggt ttctcaagta aatatacaca ttcaaggaat
    24661 cagctttaag aaggataaag ttgataaaga agaagcaaaa gcaactaaga aaaattaata
    24721 ttagccctct gaaaagtcag agggtttttt gctggtaatt gattttatat ggataattaa
    24781 acttaaagga gaattttgat tgatgaaaaa agatagggca caaaagagta caacaagaga
    24841 atatataatg aaacttatat accaaataaa tattaataaa gaagattttg aaactttaga
    24901 agataaagta gataattttt taaaagataa ttcagaacat attataaata gatataaaga
    24961 acttgcactt caatactcaa aaaatacaaa tttaaagtta gaagatactg aaattgaaga
    25021 tgttatagac aaaaaatata taaatacagt atgcaaagca ttgaaagaaa atcatgacaa
    25081 aatagatgag ttaataaata agcatgctaa aaactggaca gtagatagaa tgccaaaggt
    25141 agatgtttct atacttagat tgtctgtgtg tgaaatactt tatttagaca ctcctaataa
    25201 agtatcaata aatgaagcag tagaacttgc aaaaatatat tgtgatgata agtctcctaa
    25261 atttataaat ggtatattgg gaagtgttgt tgatgaaatt gggaaataat ataataattg
    25321 gaattgatac tagctgctat actacttcta tagcagctat ctctttagat aagaaagtta
    25381 tattcaatga aaaaataatg ttagaagtaa gggataattc aaaaggttta aggcaaagtg
    25441 aagctgtatt tcaacatatc aataatttag ggattcttag tgataggata aaatctttta
    25501 aagataagtt taatgtagaa ggagtttgct catcaaaaaa accgagacct gttgaaaact
    25561 catatatgcc tgtattcaat gtaggtcata attttggaaa attattgtct agtatatatg
    25621 gttgtaggtt ttatgagact actcatcaag agaatcatat tgaagcaagt ttattaaata
    25681 gtaaattaaa aaataataat aaatttattt ctgttcatat gtctggtggt actacagaga
    25741 tacttttaac aagcaaacaa gatagtcatc acaatgtatg tgatactaat ttaggtaaaa
    25801 ttgccaaaat aagtattaaa aaagatgata aaagtaagtt atacaataat tttggatata
    25861 atatagatat aattggagga agtaaagata ttagctttgg tcaacttata gatagagtag
    25921 gaattaagct tggatataaa tttccttcag gaaagtattt ggatgaaaat gctttaaatt
    25981 gtaatcttaa aatagaaagt ggtctgaaaa cttctgtaag agatgggtat atgaatttat
    26041 caggattaga aaatcaggta aataagatta taaatgataa tggagataat actaatcaaa
    26101 aagaatatat atctaaattg gtattagatt cagtagttag aaatatgttt aaatctttag
    26161 tgtatttatg tgaaacttat aatgtaaatg aagtagtatt tgctggtgga gtttctgcaa
    26221 gtaagtatat tcttagagaa ttaagcatga agttaagaaa aaagcatata gaagcttatt
    26281 ttacagaacc acaatattca actgataatg ctgtcgggtg tgctataata ggattgaata
    26341 actttttagg agaaagagta tgaaactaag agctttagat ataagtgaag caaactcata
    26401 tattaaaaga attttaataa acgacccaat actttctaat cttaaagtta aaggagaaat
    26461 ttctaatttt aaggttcata gtagtgggaa tgtatattta tcacttaaag atgaaacatc
    26521 aaagttaaac tgtgtaattt ttaaaagtaa ttttaatcgc aatttaaaat tagataatgg
    26581 tgtaaaaatt atagctaatg gatatatatc agtctatgaa agagatggtg cgtatcaatt
    26641 atacattaat gaaattgaaa tagagggaat aggaaattta cacatagagt ttaatagatt
    26701 aaaggaaaaa cttaataaag aaggattatt tgaccctaaa tacaagatac ctatacctaa
    26761 aatgccaaac tcaattggcg ttataacatc tccaacaggt gctgttataa gagatataat
    26821 aaatgtaata aaaagaagat atccgaaagt taatataaag ctttatccag taacggttca
    26881 aggagataaa tcagctgaag aaatctgtga agcaattaga tttttcaatc atatgaagaa
    26941 tgttgataca cttatagttg gtagaggtgg gggttcgata gaagaacttt ggtcttttaa
    27001 tgatgaaatg gtagcaagag aggtttttaa ttctcaaatt ccgataatat cagcagttgg
    27061 gcatgaaacg gattttacta tttgtgactt tgtgtctgat atgagagccc caacgccatc
    27121 agcagcagct gagattgcta ctccatcatt ggatgatata aactataaac ttggaaacat
    27181 aaaaagtagg atgagtaagt ctcttacaaa tcaaatagag ctagaccaat ataggttaga
    27241 aacagtattt aataagataa ataactattt agattcgtac acaataaaag ataaagttat
    27301 acaattagac aaaatatatg ataaaataat ttttggaata gaaaataatt taaaattaga
    27361 agatgaaaaa ttagtaaaaa ttggagcatt actccataat ttaagtccat tagctactat
    27421 ggatagaggt tatagtataa ctcaaaaaaa tggaaaagta ataaatagta taaaaggatt
    27481 aaaaataaag gatagtatag atatagtatt aaaagatgga aatttagaat gtatgataga
    27541 taaaattgaa aataaagagg gatagtatga atttaaccta cgaagaagct tataaaagat
    27601 tagaaagtat tttaagtgaa ttagaatcta aaaatgctag cttggatgaa tctttaagcc
    27661 tatatgaaga aggtataagc ctttataaac attgtaataa gcttttagat gatgctaaat
    27721 tgaaaataag taaatttaat caattgggaa tagaagaaga ctttaaaata gaggaggaat
    27781 aaaattggaa tttaaacaat gtctaaaaga aaaagcaagt tttgtagaaa aagtacttaa
    27841 agaatatatg cctaaagaag aagggtatca aaaaactgtt attgaagcta tgaactatag
    27901 cttaagtgct ggaggtaaaa gattaagacc aatacttaca ttggaggctt gtaaaatagt
    27961 tggaggtaat gaagatgaag ctattccttt tgctattgca attgaaatga tacatacata
    28021 ttcactaata catgatgatt tgccagctct tgacaatgat gatttaagaa ggggaagacc
    28081 aacaaatcat aaagtatatg gagaagctat ggggatttta gcaggagatg ctttgttaaa
    28141 ctatgctttt gaagtaatgc ttgcaggctc aatcaataaa gaaaatccag aaaagtatct
    28201 aaaagctata aatgaaattg ccaaaggtgc tggtatatat ggaatgatag gtggtcaagt
    28261 agtagatgta gaaagcgaaa ataaacaaat tggaaaagaa aaattagatt atatacatat
    28321 gaataaaact gctgcaatga tggtaggctg tatgagagca ggagctacta taggtggagc
    28381 aaattctgaa caaatggaag aaataactaa atatgcaaaa aatatcggat tatcattcca
    28441 gatagtagat gatatactag acatagtagg agatgaagct aagttaggta aaaaggttgg
    28501 aagtgatata gaaaatcata agtcaacata cccatctctt ttaggtttag ataaatctaa
    28561 agaaattgca cataatttga ttgatgaagc aaagaaaagt atagaaaaat tatctgatga
    28621 tgtagatttt ttaaagggat tggctgaata tataatagat agagaatact aaaatataca
    28681 gctaaaatcg aggagggaaa aatggatttt ttttcggaga tttttaacaa tggggctttg
    28741 ggaataagtc tgattgcttg tttcttagca caatttataa aaatatttac tggtaaggaa
    28801 aaaagaattg agttatcaag aatacttatt tcaggaggta tgcctagttc ccatagttct
    28861 tttgttacaa gtttagctac agtggtaggt attgaaaaag ggtttaattc tactgatttt
    28921 gctataatta ccgtgcttgc cttaattata atgtatgatg cagcaggtgt tagacgggct
    28981 gttggtaaac aagctactat attaaatcaa atggtggcag atatacagca tggaaagcat
    29041 atagagcaga agaaattaaa agaattaata ggtcatacac cactagaagt ttggtttgga
    29101 gctttacttg gaatagtaac agctttaata ttgatgtaaa ttgaaaattt acagaataaa
    29161 ggtgaggata atatgtataa atatttagat aaagtaaatt ctccaaagga tataaaaaat
    29221 atgagtatag aagaaatgga tttacttgca aaggatataa gaaagttctt agtaaaatct
    29281 gtttcaaaga caggaggaca cttagcttct aatctaggag tggttgagtt aacattagca
    29341 cttcataaag tatttgatag ccctaaagat aagattgtat gggatgtagg gcatcaatct
    29401 tatgttcata aaatagttac tggaagaaaa gattgttttg tatctttgag acagtttaat
    29461 ggactaagtg gttttccaaa ggagaatgaa agtcctcatg atatttttga tacaggacat
    29521 agcagtacat ctatttctat agctacaggt attgcctgtg ctagggatat aaaaaaagaa
    29581 aactatagtg ttatatctgt gataggagat ggttctataa caggaggtat ggcacttgaa
    29641 gctttaaatc aattaggata tatagatacc aatatgatag taatccttaa tgataatgag
    29701 atgtccatag ataaaaatgt tggaggaatg tctaaatatc tgtcaagtat tataagaaac
    29761 tcaacagttg agaaaatgac agatgaagtt gataaaattt taaatgttac acaaacaggt
    29821 gaaattttat ctaagactgc acatagattt aaagataaac taatgtatag cttttctcct
    29881 caggactgtt cattttttga ttcattaggt ataagatatt atggacctat agatggccat
    29941 aatactaaag aactaataga tatattgaga aaagctaaac acaaaaaagg gcctgttctt
    30001 ttacatgtaa ttacgaaaaa aggaaaaggg tacagatttg cagaagaaca acctgataaa
    30061 tatcatggag tatcaaagtt tgatataaag acaggtgtta catctgcaaa agttaagtct
    30121 atgtcaatta gtgttggaga aaaacttgtt gatatggcta atagtaatga aaatatagta
    30181 gcaataacag ctgcaatgcc atctggtaca gggttaaacc tgtttgaaag tgcatatcca
    30241 aaaagatatt atgatgttgg tatagctgaa caacatgcaa caggatttgc agcaggtctt
    30301 gcaaaaaatg gtatgaaacc ttactttgct gtgtactcat catttttgca aagggcatat
    30361 gaccaagtta ttcatgatgt atgcataaca aaaaagccag ttacttttct tatagataga
    30421 gctggattag ttggaaatga tggagaaact catcatggta tgtttgattt aagttactta
    30481 aattctattc caaatattgt agtaatggct ccaaaagaca caagagagat ggagcttatg
    30541 atggatttgt cattaaaatt agattgtcca ctagcaatta gatacccaag aggaagtagt
    30601 tattacctag ataagggaga gtatggagag attgtactgg gtaaatatga ggtattagat
    30661 gacggtcaag atacagtcat actttgtatt ggaagtatgg taaaacatgc tttagaggct
    30721 aaagaaatat tatcaagaga aggaataaat ccgacaatag ttaatgctag attcttaaag
    30781 ccaattgatg aaggtatgtt aaaagcatta ttaaaaaatc ataaaaatgt agttactata
    30841 gaggataata ttgtaactgg aggatttgga agtagaataa ataagtttat tatagataat
    30901 gaatataacg taaatatact taatatagca ataccagaag aatttgtaaa acatggcaat
    30961 atagatgaat tatatgattt tgtaggatta tcgccaaaaa gtattgctga taaaataagg
    31021 aaattagtta ttgagtaaaa atctaatgta gatattgtaa attaaaattt atataaaata
    31081 tagaatttat caattagaat aaatatcaag tgtgttactt caagctactt tagttatcat
    31141 taaagtagct tttaacagaa aggtaattgt atgaaaaaaa gaatagattt attattagta
    31201 gaacaagggt actttgaaag tagagaaaga gccaaaaaag caataatggc tggattggtt
    31261 tttgttgata atcaaaggtg tgataaggct ggtacagaag taaaagaaga ttgtagtata
    31321 gaagtaaaag gaaatccaat accatatgtc agtagaggtg gtttaaagct tgaaaaagca
    31381 atgaaaaact ttgatttgac tattgatgga aaggtatgta tggatatagg ggcatccaca
    31441 ggtggtttta cagattgtat gctcaaaaat ggagcaataa aagtattttc tatagatgtt
    31501 ggttatggtc aacttgcatg gaaattgaga caagatgata gggttgtgtg tatggaaaga
    31561 accaatataa gaaatgtgac aatagaagat acaaagcagt ttgctgattt tgcgtcaatt
    31621 gatgtgtctt ttatatcgtt aaagttagta cttccaaagg caaaggagtt agtacgtcat
    31681 gatggggaag ttgtcgcttt aattaaacct caatttgaag caggaagaga aaaagtaggt
    31741 aaaaaaggag tagttagaga aaaatctaca catatagaag ttataaaaat gatttctgat
    31801 ttctcagtcg aaaatggatt tgagatttta gggttagatt tttctcctat taaaggtcct
    31861 gaaggtaata tagaatatct aattcatttg agaaatggaa atgaaggata tgaatttgat
    31921 ggtgagacat acaataacaa aatagtagaa gttgttgaag cttctcataa tttagacaaa
    31981 taggttaaaa gggggcttag atttgatcct cgaattatat atgaaaaatt gtgccttagt
    32041 tgaagaacta aggttgaata ttgataaaaa cttaaatata cttacaggtg aaactggttc
    32101 aggaaaatct ataataatag atgcacttgg actttgttta ggtgataaat atgataggtc
    32161 ttttttaaga aaaggaacag ataagggact tgttgaggct gtattttttt cagacaatag
    32221 atatcttaaa aaaatattag aagaaaatga tataagtatg gaagatgata atttattggt
    32281 aataactagg ttgatatact cagatggtaa gagtactgct agagtaaatg gaaggactgt
    32341 taaagtgtct cttcttaaag aaattgcatc tacacttata gatatacatg gtcaacatca
    32401 aaatcaagct ttgtttaata aagatactca tttaaaattt ctagaccttt ttggagaaaa
    32461 tgaattggaa gagtttaaaa ttgcttataa aaatgtgtac cataagtata gtgaagtaaa
    32521 aaaagcttta aattgcttga cagaaaataa agatgagatg caaattcaac gagaaataga
    32581 tttattaaga tttcaaataa atgaaattga agctgcaaat ctaaataaga atgaatatga
    32641 agatttatta aaacaaagag aagtttatag aaatagtgaa aagatatata ataatctaaa
    32701 ttcgagttat agcaagttac ataatggtga gtataatgtt attgacctaa taggtcttgc
    32761 atctaaggaa cttaatgaca tatctaaata tgacagtgtt ttaagtgaat atagtgatac
    32821 tgtagaaaga atcatgtatg agttacaaga catatctggt gagataagaa attacaaaga
    32881 taatatagac tttgaacctt atgagctaga gcagattgag cttagaatcg acgagattaa
    32941 taacttaaga agaaaatatg gagacagtat agatgatata tttgagtatt atggaaaaat
    33001 taaagataga ttagatgaaa tattaaacag agatgaaaga gttgaacagt taagaagcaa
    33061 actgatgaat atagaagaag atttaaaaat aaaagccagt aaattgacta aagcaagaat
    33121 agaagtagca actactttag aaaaaatact tcttgatgaa ttaaaaagct taaatatgaa
    33181 aaatgtaatg ttcaaggtta attttgaaaa aggtccattt actttagatg gagtagatga
    33241 tattgaattt atgatatctt ttaacttagg agaagatatt aagcctatat ataaagttgc
    33301 atcaggtggt gagatgtcca gatttatgtt agcgtttaaa accatattgg ctgatataga
    33361 tgatattgat acattagtgt ttgacgagat agatactgga ataagtggga tagcagcaca
    33421 gatagttggt gagaaattga gtgatatagc aaagaaaaaa cagataatat gtataactca
    33481 tttacctcaa attgctgcaa atgcagacac tcattattgt atagagaaag atacttcaaa
    33541 taataggacg tttaccaatg ttagtaagtt aaatgaatct caacgaaaaa atgaaattgc
    33601 cagacttata gcaggtaaca atataacaga aaaaactatt gaacatgcaa gtgaaattat
    33661 tgagttggct aaaaaatgtt agtgaaaaac aagcatttta ataggtgtaa agaattttta
    33721 tagaaatgct ttacatctat tttttatgat gaaattattt taaaatatct agtgcctata
    33781 ttgaagaaaa gtagatttaa aaatagaaga aaatttgtat aaatttgtaa ctttgaaatt
    33841 tctaaatgct taagtataat attatttatt aaggtagtag tgagggggac ataaaatgaa
    33901 taaaaaagat actatgcact taatttttac aattgtaatt tttattttat tatggtatat
    33961 tgtggtagca gtaagtgaga ttaataaaaa tattgaaagt tgtagtaata aattagatta
    34021 tttgtcacaa gagattatga gtactaaatc ggatattagt aatactataa atgaagaaat
    34081 gtcaaaaagt tatataacaa agagtattga cttaaaagta aaaaaaatcg agaaaaaaga
    34141 gtgtgttatt gatgtagatg ttcaattaag taaacttgga aaaaatggaa aagtattttt
    34201 tatgtataaa gaagatagtg ataaatggaa tgaaactgag atgacaaaac atggagaatt
    34261 atcatatgca tgtgaaatta agataaatac aggaagtgaa tatgactata aagtagttac
    34321 aagtggagct atatctgaaa gttctgatgt gcaaaaattg actaaatctg attatatgcc
    34381 tgaacaaccc atatttaatt ctgggataag agataataga gaatacttta ttgaaatagt
    34441 agaaaatagt aatcttttca atcatgagag tgaaaagtca aaaaataaag tttatgataa
    34501 tattagatta gaaaaagtag atattattgt aaatgaagga aaaaaagaca ctatatacaa
    34561 agccaaatta gctgaaggct tagatgatgg caaaactaat aattcaaata gaaatcaagt
    34621 gaattatgaa gtaagttttc ctaaaaggga tattgatgac ataatttata ttaaagctaa
    34681 acttacatac aataatggag tagaatatac aaaggatata actgaagata taactatggg
    34741 attacaagat ttagaaaaat aaatacatat ttaaataaat attcaaatag atttgttcaa
    34801 taaaatagag tataaataaa actgtagtag ttttacttat actctattaa aaaattataa
    34861 ttttaattcc atatatctag ccccttcaat tggattataa gtataaggtt ctatatcata
    34921 aaaaccaaac ttttcatata atccttgtgc acttttcatg cttggaagag tatctaatct
    34981 catgtgtgta tatccaaatt ttttagcttc ttcaatgatt tcttccaata gtatttttcc
    35041 tatttttaat cctctaaatt gatttcttac atataatctt tttaattcac atattttacc
    35101 ttcaagtttt tttaaagcaa cacaacctgc caaattttca tcaacaaatg ccaatatcaa
    35161 tgagcctgaa ggttttttat attttcctgg aagtgttttt aattcattat taaagtcttg
    35221 aaaacataag tctatattta gagaatttga atattcagta aatagaagtt ttacgttttc
    35281 aaggttttct aaatcttttt cttcaacaaa ttgtaaagtt atattttgag ccattttatc
    35341 cgccattata taaacatgtt tatataatga cttcacctcc ttgatatatg tatatttttc
    35401 ttaatatcaa ttagatttta ataccattac tttttcacca ccatagtcaa gctctccatt
    35461 aaactcaaat ccaaaatctt tataaagctt aatagccatt ttattgtctt cgaatatact
    35521 aagatatagt tcatcacagt tatattcatt tttaagatga tttacaagaa aatctaatga
    35581 tgcttttcca tatcctttac cttgatattt tgaatcaatc ataaatctat caagccaaac
    35641 tctatcatct tctaaacgac catacatagc aaatcctatt aaagaatttt catcatatat
    35701 tccaacaggt acccaagctg ttataaattt agattcagca atagaaagtg cattgctttc
    35761 tatataatct aactgacttt tatctacaga caaactaact aaatcatgcc aattatttat
    35821 gtctgcaatt cttacattta atttcaagat attaccgccc ttcaaacttt ttgttacaat
    35881 ctaatactat catattatgt attaaaaatc acttattaag attttaaata aactttttaa
    35941 atgaataaat tctcaggaaa aaggaattat aaatacataa taatacagca taggagtaga
    36001 tggatatatg aacaaaataa ataaaaattt caaaaatatt tggataatat taggagctgt
    36061 atcgattttt accatggtta taatttttaa taaaaattgt aatgttaata aagagactca
    36121 aatagaagct tttacaatta agaatcatga taaaaaattt gtatatccta tgggaaatat
    36181 aattggagta aaagcaaata cagatggtgt attggtctta ggatatgagg aagaggatgt
    36241 agactatatt ggaggcatac aaatcggtga taatatagta aaaatagata ataaaaggat
    36301 aaaaaatagt caagatgtat cagagatttt gaataagatt aaaaaaagta aagttgaagt
    36361 gacttttgag agaaaaaata agtataaaac agaagtaata gagactaaaa aagaaaatgg
    36421 caagtataaa cttggattat gggtaagaga taaaatatct ggaattggaa ctatgacttt
    36481 ttacgaccct agtatggaaa agttcaaagc aatcggtcat gccataaaag attcggatac
    36541 taatgaacta ttaaagataa agcaaggata tatatataag ccagaacaat taaaaatagt
    36601 aaaagctagt aatgaaaaag taggtaaaat aaaaggagat tttaatgaca gcaatttaat
    36661 gggtaatttc tcaaataatt cagagttagg tataagtggt aatattacag aaaaccataa
    36721 taaagagttt aatgtagcaa ataaagaaaa gcaattaata gaagttggaa gaccgcaaga
    36781 tgtaaaaata ggagatgcag taattctttt tgaggataaa aacaaaaata taacaagcta
    36841 tgatataaaa atagaaagta tagtatatga taaaggaaat tatagagata tggtaataaa
    36901 agtagtagat gacaagttat tggaatacac aggaggtatc gtacagggga tgagtggagc
    36961 tccaataata caaaataata aaattattgg tgcaataact catgttttta gagataatcc
    37021 gaaaaaagga tatggtattt ttatagatga aatgataaaa ttgtaggtga ggcattaaaa
    37081 aattttatta ttttatcaat tatctaggag gaatataatt ttggagtgtc gaatatgctt
    37141 tagagtagat aattaggaag caattgtgta aaaagtttag ttttctgtaa taagaagatg
    37201 ttttttaatg gggggatttt tagtggaaaa aatcaaaata gttttagcag atgacaataa
    37261 ggatttttgt caggtattaa aagagtattt gtctaatgaa gatgatatcg atatattagg
    37321 catagctaag gatggaattg aagcattaga cttagtaaaa aagacacaac cggacttatt
    37381 aatactagat gtaataatgc cacatctaga tggattaggt gtaattgaaa aattgaatac
    37441 tatggatata cctaaaatgc caaaaataat agtactatca gcagtaggtc aagataagat
    37501 aactcaaagc gcaataaatc taggagcaga ttactacata gtaaagccat ttgattttgt
    37561 tgtgtttata aacagaatta gagagctagt ttctaataga gttacacaag ttgagccaaa
    37621 acctagacca gttcaagaga ctcaaatgac tagaagtgat tttgttaaga atgtaggaaa
    37681 tatagagaca gaaatcacaa atataataca tgaaatagga gtaccagctc atataaaagg
    37741 atatttatat ttaagagaag caataaaaat ggttattgat aatgttgagc ttttaggtgc
    37801 agtaacaaaa gagttatatc caagtatagc taaaaaattc aatacaacac caagtagagt
    37861 tgaaagagca ataagacatg caatagaggt tgcatggagt agaggaaaag ttgacacaat
    37921 aaatcaatta tttggatata cggtacacaa tactaaagga aaaccaacta attcagaatt
    37981 tatagcaatg attgctgata aattaagatt agaacatagt atggttaaat aaacaagaca
    38041 taaaaagtaa ggctttttaa ttaaggcatt ggctataaat gcgtattaca agcagcgaaa
    38101 cggtataacc actagggtta taccgtttcg ctattttaat aaatataaaa attttcttta
    38161 ttatttgctt actatatcaa tataataatt ttattatact atggatatag tatgtgtctt
    38221 tacaagttgt aaactgacag tggtttattt tttaatataa atattgactt tgatgcaggt
    38281 aaactttgta tttttaagcg tattatggaa tatgttaaat aaaaaaatga tgaaatatag
    38341 tattgtaaat gccaaagatg caaaacaaac ttaaaacatt tattttattg ttaagtaatg
    38401 ctataatata atgtgatttt aataatgata gtggaggttt aaatatgaga gtcgaggccc
    38461 ctataaaagt agatcgaaaa accaaaaaac ttgctaaaag agttgaaagt ggggaaatag
    38521 cagttataaa tcatatagac atagatgaag ttgctgcaaa ctctttagta gaagctaaaa
    38581 taaaacttgt cataaatgcg gctccttcta taagtggtag gtatcccaat aaaggtccag
    38641 ggatattaac tgaaaataat atactaataa ttgataatgt tggagaagag ttatttgaag
    38701 aattaaaaga aggagaaact atagaagttg tagatggaaa gatatataga aaaggaaaat
    38761 ttttaggtgc aggagaagtt ttagacaagt atgaggtttc acaccaaata aaggctgcct
    38821 atgagaattt agctgtagaa ttggatagat ttatagataa tactatagat tatgctaaaa
    38881 aagaaaaagg atttatactt ggagaagtag aaattccaaa agtgaaaact aattatgcta
    38941 ataaacatgt attgatagtt gtaagaggtc aggactacaa agaagattta agtactatgc
    39001 tttcttatat agaagaggta aagccagtat tggttggtgt tgatggagga gcagacgctt
    39061 taattgagtt tggatatact ccagatgtga ttgtaggtga tatggatagt gtaagtgatg
    39121 aagctttaaa aaaggctagt gaaatagtag tacatgctta tacagatgga agagcaccag
    39181 gtcttaagag ggttgaggaa ttaggcttag atgcagttgt atttcctgcg ccaggaacta
    39241 gtgaagatat agctatgctt atagcctatg agtataaggc tgagttaata gtagcagttg
    39301 gtacacattc aaatatgatt gattttcttg aaaaaggaag aaaaggtatg gcaagtactt
    39361 ttttagttag actaaaaata ggttcaaagc ttatagatgc taaaggcgtt aatttgttat
    39421 acagaagtaa attaaaaata aagtacatat gggcattaat agcaacagca ttatttcctg
    39481 tgttggtagt tgcatcattg tcaccaggag ttcaacagtt catacaacta atgcaattaa
    39541 agtttagagt attattacag atgtaaattt taggaggata aatatgcata ttaatatgaa
    39601 atattatata gtcactatag gggctatatt tatagctttg ggtataggca tgttagtagg
    39661 atttaattta aataataatc aacaattaag tgaacaacaa gcaaatataa taaatgattt
    39721 ggatgataag tttaatatct taaaagaaaa aaatgataaa cttgacagtg acttagcaag
    39781 tgtaaataga gattatgaag aggcagttaa ttttataaat aaaaatgtag ataagatttt
    39841 agctggttcg ctgaatggta agagtatagg aataatatct gctaatgaaa atgacgatta
    39901 tacaaaaaat atagaagaca taataaataa atctaatgga agcattgcat ttaatataat
    39961 attaaaagaa aacataacaa atccagaaaa gttaaaggaa atgtcaacta aactaggtac
    40021 agatgtaaaa aatgcaaatg atgcagttaa ttatataatt gacactctta aaaaggaaga
    40081 tgcaagtgac attttaacat atcttcaaga actagatgta ataaagttta attttattgg
    40141 agatacatat ctaaaatatg attccgttgt aatagctggt ggaaatgatg caaaagacag
    40201 tacaaaacag tttgaaaaaa tagaaaaatt tgtagtttca aaacttaaat cagagaataa
    40261 atatttagta gaagttcaaa atacaggagt taaaacttct tatgtagaac tatattctaa
    40321 aaacaaagtt gcaactatag acaatattga tgaaggtata ggaagtatat ctctagcgat
    40381 tcttctacaa caaggtaata tagttggtaa ttttggtaga ttggatactg caacttcatt
    40441 gttgccatct attaaataga aataatttta aggagagaaa aaatggattc ttatataagt
    40501 ataataatac cagcttataa tgaagaaagt agaataaaat atacattaga aagcatattg
    40561 gacattaagc aaataaatga aataatagtt gtggatgatg gttcaattga caatactgct
    40621 aaggttgtat ctgagctaga aaatgaaaag attaaatttt ttaagttaga taaaaataga
    40681 ggtaaaggat atgctttaaa ttatggtatc aagttagcaa tgaaaaatgc agatattata
    40741 ggttttctgg atggagattt aggaagttct gctaaagaag ttgaaaaatt aatagtacct
    40801 atattaaatg gtgaggcgga tgttacaata gccaagtttc ctcctgcaaa gagaaaagga
    40861 ggtttgggat ttgtaaaagg tttagctaaa aattctgtgt ttgagatgac tggtgttgag
    40921 ttagatgcaa ctttatcagg tcaaagaata tttaaaagag aagttttaga aaagtttgat
    40981 gaaatacctt ttggatatgg agtagaagtt ggaatgacca ttgatatatt aaagtatgga
    41041 tttaaaatta aagaagtatt agtaaatatg actcataatg aaacaggaag aaatttaaaa
    41101 ggttttattc atagagggaa acagtattat cacataaaaa aggttttagg acaaaaaaag
    41161 aaagaatgga ggtaatatct tagtgaaaga ttatatactt tataccattt tattattaac
    41221 tggattattt ggaacctatg ctgtaattcc actatttaaa aatttattga taaatggaaa
    41281 tgtacttaga ccaaactata aaaaagatat gataccagta agtatgggaa ttgtattttt
    41341 gcctatgatt gtaatcaatg gaataatact tgctttcttt actacagaat ttaagaattt
    41401 agcgtatata tttatgttta tgtttggtat gatatccatg ttttttgcag ggatactaga
    41461 tgatgtcatt ggaaatagag atgtaagtgg tttaaagggg cattttaaaa gtttatttaa
    41521 aggaagtctt acaacaggag gttttaaagc tttatttggt ggatttgtag ggcttatagt
    41581 ttctgttgct atatcaaaag acataattga tataatagta aatacactaa taatagctct
    41641 atcaactaat ttaatgaatc ttttggattt aagaccagga agggcaataa aagcatattt
    41701 attcataatg gtaattgtat ttttaacatt gacaggattt gttcaagttc ttccattact
    41761 tatagtacca aatgtacttg catactttaa ttatgattta aaagcaaggg ctatgatggg
    41821 agatactggt tcaaacgtcc ttggaatatc tattggaatg ctcatttctt ttgggtatcc
    41881 atttaacatt agattaggat ggttaatatt tgtagttatt atgcatatat tcactgagaa
    41941 atactcatta actaagataa tagaaaaaaa taaactatta aattttatag ataaattggg
    42001 aaggtagtat tatgataagc aaaagagtgg ggattgttga atcaatagta agtcaaactg
    42061 aaactctaga tgatataaga gtaaatataa atggagaaat acaaaaggca tataattatc
    42121 caaaaatatc tggaactata aatataggtg acgaagtggt tttaaataca acagcagtag
    42181 aactgagctt aggtacaggt ggataccatt ttgtaataac taatttaaat aatatagaat
    42241 ctactttaac tgaaggtgga catataatga aacttagata tacgcctctt caaataaaag
    42301 tggattctgt tgaggaacaa gaaagtattt atcatgataa atttgcgaat tttaaaggat
    42361 tagaaggtct ttctgttgta gtagggactc ttcatagtat gttaactcca tttgcagctt
    42421 catttaaaag aaataatcca aataaaaaat tagtgtatat aatgactgat ggagcatctc
    42481 ttccaattta tttaagtaaa aatgtggata ctttaaaaga aaaaaaatta atagatagta
    42541 caataactat aggaaatgcg tttggtggag attatgaatg tataaatata tatactgcat
    42601 taattacagc aaaagaaata ctaaaagcgg atgcagtatt tgtcagtatg ggacctggaa
    42661 ttgctgggac aggaacaaaa tatggattta cagggattga acaaggctct attcttgatg
    42721 ctgtaaaaaa acttgaagga agagcaattg caattccaag aattagtttt gctgacaaac
    42781 gagaaagaca tcaaggtatt tcacatcata gtatgactgt atttaaagaa attgtaaatg
    42841 taaatgtaga cataccaata actatatatg atgatgaaaa attgaacttt ataaaagagc
    42901 aaatcaattt aaatggttta gacaaaaagc ataatataat ttatatagat aataataaaa
    42961 cgaaagagga tttagactat tttaatctaa aagtaaagag tatgggaaga aattatgaac
    43021 aagatgaggc atttttcaaa gctgcaagca cagcagcata ttacttaatg gaggtttaag
    43081 atggtattag aagaaaaaac tattagtagt gatagggttt atacaggtaa agtaattact
    43141 ttaaaggttg atactgttga aattccaggg caaggatatc aaaaaaggga gttagtagaa
    43201 gttggtggag cagtaggaat agttgcaatc acagatgata ataaagttgt gttagttaag
    43261 caatttagaa aacctataga aaagccaatc tttgaaattc cagctggtaa actagaaaaa
    43321 aatgaaagtc caaaagaatg tgctgaaaga gaattaaaag aagaaactgg gtatagtgca
    43381 aaaaatatta agctaataca taaatttttc acttcagcag gtttttctaa tgagataatg
    43441 tttgtatatt tagcaacagg tcttactcca ggagagaata atttagatgc agatgagttt
    43501 ttagatgttt atgaaattga gcttgaagaa gcatataaca tggtattaaa aaatgacgtt
    43561 gaagatgcaa aaacatctat tggattatta ttagtaaaag atatgtttaa aaattaatat
    43621 ttataaaatg aataaccctt tgtcctaaag catattattt tataaaggac aagggggaat
    43681 taaattgaga agaacatata gaaaaaatga ttttagagaa ttaaatagac aaataattat
    43741 tataggattg ttgtttatga tgtctattgt tgttgggtct tacataaata aaatattgcc
    43801 aggttctagt aacaatatat taaacaatat aaatcctgct gtagagtatt ataatctaaa
    43861 tataagcata aaagatacag tgatacaaaa tttaaaatca gatgcaatct ttatgggctc
    43921 aatagctcta ttaagtctat ttgtagtaac aattccagca gtgttggtag cttttgtatt
    43981 aaaagggatg tcaattggtt atacaataaa tagttgtata ttggcattaa aatttaaaag
    44041 tataaaaatg attttaataa tattatttaa aaatttgatt attattccat gtgcaatcat
    44101 attagcatta attagtttaa gttattttaa agaaatggtt tatgaattta aaaagaaaag
    44161 tagaaaaaat atgcaatttt taattaagag gtatatacta aatattataa ttataatagc
    44221 actatcattg gggttgcaat taatattaaa tactgcaagt attggtatta ttaagttcct
    44281 agctaaataa aggagtttat aatggatatt atagagggat atatagatta cataaaagat
    44341 aaaaaaaaat tatcagagaa tacagtggca tcatacttta tggatataaa aaaatatatg
    44401 gaatatataa atcaaaaaag cattaaacta gtagatatag tagaaaatga tattataagt
    44461 tacttaataa agttggaaaa agataatgta tcaatagcta caatagcgag gatgatatcc
    44521 tctataaaat cttttcatga ttatttattt ttaaatcata tatgtacaaa taatcctgca
    44581 aaggatataa aaaaacctaa aataaaaaaa gaaaatataa atatacttac agaagaagaa
    44641 attgaaagac tattaaattt tccaaagtta acaacaccta agttgattag ggacaaggct
    44701 atttttgaag tattatatgg tactggtata aaagtatctg aattagtaga gatgaatata
    44761 gaggatattg acctagatat tgattatata tattgtaatt catgttgtaa aaatcaaaga
    44821 gtgataccac tttgtgatat tacaaaactt tacttagaaa agtatctaaa agaagcaaga
    44881 cctaaaatgg ctgcagaagg tgaaaaatca ttattcgtaa gttcattagg tcaaaggttt
    44941 acacgacaag gtctttggaa agtcataaaa aaatattcta atctagccaa tatagataaa
    45001 aatataaatc ctacaatgct tagacattca tttgctattc atttgttaaa tgaaggtgct
    45061 aacattgctg ttgtaagtaa aattttagga aatgttaatt tatctagttt gcaagtatat
    45121 ttaaatcata tagataaaaa cgtgagaaga gaaataaagg agaaacatcc tagaaatgat
    45181 gttgatgtag aattacaaaa agcagaagct aaatagacta tatagcaaat tttataattt
    45241 agaaggagca tatttatatg agcagagtaa tttggattgt aattgatagt gttgggatag
    45301 gagcattacc tgatgctgaa aaatttggtg atagtaagga tgtaagtacc cttggaaata
    45361 tatttaagga atatccagat attcagattc ctaatatgag aaatttaggt ataggaaaca
    45421 tagaaggtat agattttttt gaaagtataa aagaaccaat aggttgtttt ggaaagtgta
    45481 aggaaatgtc tcagggaaaa gatactacta caggtcattg ggaaatgact ggtataatag
    45541 ttgacaaacc atttaagaca tttgaacatg gattctctaa ggagataata gaagagtttg
    45601 agaaaaaaac tggaagaaaa gtagtaggaa ataaaccagc ttcaggaact gtaataatag
    45661 atgagtatgg agagcatcaa ataaaaactg gagatgtaat agtgtataca tcagctgata
    45721 gtgtatttca gattgctgct aatgaagaag taattcccct agaagaactc tataatatgt
    45781 gcaaaatcgc tagggaaata atgatggggg ataatgcagt agctagagta attgctagac
    45841 cattcatagg taagaaaaaa ggtgagtttg tgaggacttc aaatagaaga gattattcac
    45901 tagacccatt tgaaccaaca gttcttgata atataaaaga atcaggactt gatgttttag
    45961 cagttggaaa aatagaagat atttttaatg gaaaaggaat tacagatgct atccacacta
    46021 aaagcaatat ggatggagtg gatgaaactt taaattatat gaaacaagat aataaaggtc
    46081 taatatattc aaatctcgtt gattttgatt ctaaatatgg tcataggaga gacccagaag
    46141 gatataagaa agctcttgaa gagtttgata gtagacttcc tgaaataatg gctaatatga
    46201 gagaagatga tattttaata atcaatgcag accatggaaa tgacccaaca tataaaggta
    46261 cagaccatac aagagaatat atacctgtta tgatttatgg caataagatt aaaaaaggtt
    46321 ttaatttagg agtaaaagat acttttgctg atattggagc aactgttgca gatatactaa
    46381 atgtaaagtt gcctaaacat gggtcaagct ttaaagaaga tttattttaa actataaaat
    46441 gataaaaact tagcttaata ggatggagag attggaatgt ttgaaaagat agcacaaagt
    46501 agtaaattta taaattctaa aagtaatata aaacctaaga ttggtttgat attaggttct
    46561 ggactaggtg atttagcaaa tgatatagaa gaccctgtca caattaaata tagtgaaatt
    46621 ccaaacttcc ctgtatcaac agtagcagga catgctggac aacttgtaat tggaaaactt
    46681 gaaggaaaag aagttattgc tatgcaaggt agatttcatt actatgaagg atattctcag
    46741 aaagaagcta cttttccagt aagggttatg aaagaattag gagtcgagat tcttatagta
    46801 actaacgctg ctggaggcgt aaataaagaa tttaaagcag gagatttgat gcttataaga
    46861 gaccatataa actttagtgg aagtaatcca ttagtaggaa aaaatgatga cagatttggt
    46921 gctagatttc cagatatgtc agatgcatat tcttctaaat atgtagatgt agttaaaaac
    46981 tgcgctaaag aatgcaatat agatgtcaaa gaaggcgttt atatgttttt tagtggtcca
    47041 aactatgaaa caccagcaga agtgagaatg gcacaaattt tgggagctga tgctgtaggt
    47101 atgtctactg ttccagaagt catagtagct tctcactcta atataggagt tattggtata
    47161 tcttgtataa caaatatggc tgctggcatc ttaaatcaac cactaagtca tgaggaagta
    47221 atagaaacta ctcaaaaagt taaatcagaa tttatgaatt tagttaaaac aacggttaaa
    47281 aatttataag ttaaagctta ttatagattt tttgaggaag tttataataa aatagttaat
    47341 gtttaatttg aaaagaatta ataaaaagct gtcttgatat agaaagttat ttctaataaa
    47401 ggacagtttt tttataatac tacaacattt gaaagtgttt taaagtttaa tatttattta
    47461 aattattata ggttagaatg gagatttcaa ttatgagaat gtatgatatt ataaaaaaaa
    47521 agagagataa cctagagctt agtaaaaaag agataaacta ctttatagaa aactattcaa
    47581 agggagatat acctgactat caagcttctg cactattaat gtcgatttat ttaaataaga
    47641 tgaataaaca ggaaactgta catcttacag aagcaatgat gaactctgga gacatgataa
    47701 acctatctga gatagatgga ataaaggtag ataagcatag tactggtggt gttggagata
    47761 agactaccat agctcttata cctttggttg cttcatgtgg agcaccagtt gcaaagatgt
    47821 caggtagagg tcttggtcat actggtggta ctattgataa attggagtct ataccaggtt
    47881 tttcgacaga aatggagata agtaaattta ttgattctgt aaataagtct aaaatagcag
    47941 tttgtggtca aagtgcaaag gtagcaattg cagataagaa aatatatgct cttagagatg
    48001 ttacagcaac tgttgataat atatcattga tatcatcaag tgtaatgtct aaaaagttag
    48061 cttcaggagc tgatgcaatt gtgctagatg taaaaactgg tgatggagca tttatgaaga
    48121 cattagaaga gtcttttgag ttagctaaat ctatggttga tataggtact ggtatgaata
    48181 gagatacaat agggattgta acagatatgg atgaaccttt aggttttgca gtgggaaact
    48241 ctttagaagt tatagaggca attgagacat taaaaggaaa tggacctaaa gaccttgttg
    48301 agctatgcga agtattagga gcatatatgt tggttttatc aaaggtagca catgattttg
    48361 aagatggtgt tgaaaaaata agagaatcaa taaaatctgg tactgctata caaaaactca
    48421 aagagtttat agagaatcaa ggtggtaata aagatgtagt agatgattat tctttattca
    48481 aaaaatcaaa gtataaatat ccagttaaat caactaaatc aggttatata agcaaaatta
    48541 aagctgaaga tgttggtgta tcggctatga ttcttggagc aggtagagaa actaaagatg
    48601 atattttaga tttatcagca gggataattt tagaaaagaa agtaggagat tatgtaaatg
    48661 aaggtgaagt tttagcttac atgtattata atgatgaaca aaagctttca ttggcaaagg
    48721 gaagatttat tgattcctac tctattgtag attcaaaaat agaaaaaaat aaattaatat
    48781 atggtattgt aactaaagaa gagattaaaa agttttagaa aattttagaa aagtgattat
    48841 aaaaggccta acatgtcaaa aataatgtta ggttttttat tttttgataa actataaatt
    48901 ataccaaaaa tatatatttt aaaacatttt ttacaatata aataagttta ttctacaaga
    48961 tgattgaaaa ttaaagtatc
//
LOCUS       DAMJYX010000020        46013 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJYX010000020 DAMJYX010000000
VERSION     DAMJYX010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.020"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(15..1160)
                     /locus_tag="PC479_001236"
     CDS             complement(15..1160)
                     /locus_tag="PC479_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HDF2803789.1"
                     /translation="MATKLEENAKLREVIDTLETTKDDLQTVKNNIADVLGLPFTGND
                     KLETTKNTLSSIRSTFVSNLSKKGVSTVSNAPFKTLAENVGKIEQGNMIVPPWYVPKN
                     IFIKALSTAVKDKTPCVSAEKKIYFINGYTGTSGLNNLCYDTVLNTTTTKNIPSSFVE
                     SALFFYDGYIYKIGGFSGYSAPTAICQKYNILTSSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSTYKIVVFGL
                     KSSISSHYFSTINYDPVSGTYSYAGEGFDSNTFAVQLGSTLYVSDYGYKAGSGKCGVV
                     IFSSSGIKKLTFDKGAVPNYSYFGASVDGRQIYFILNSEITCFIPEL"
     gene            complement(1177..2253)
                     /locus_tag="PC479_001237"
     CDS             complement(1177..2253)
                     /locus_tag="PC479_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2803790.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRFTSDKLDGVLE
                     EIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(2265..2882)
                     /locus_tag="PC479_001238"
     CDS             complement(2265..2882)
                     /locus_tag="PC479_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2803791.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(2875..3924)
                     /locus_tag="PC479_001239"
     CDS             complement(2875..3924)
                     /locus_tag="PC479_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2803792.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(3925..4344)
                     /locus_tag="PC479_001240"
     CDS             complement(3925..4344)
                     /locus_tag="PC479_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2803793.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(4349..4609)
                     /locus_tag="PC479_001241"
     CDS             complement(4349..4609)
                     /locus_tag="PC479_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2803794.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(4623..6515)
                     /locus_tag="PC479_001242"
     CDS             complement(4623..6515)
                     /locus_tag="PC479_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2803795.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(6508..7209)
                     /locus_tag="PC479_001243"
     CDS             complement(6508..7209)
                     /locus_tag="PC479_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2803796.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            complement(7225..9618)
                     /locus_tag="PC479_001244"
     CDS             complement(7225..9618)
                     /locus_tag="PC479_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2803797.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(9683..10198)
                     /locus_tag="PC479_001245"
     CDS             complement(9683..10198)
                     /locus_tag="PC479_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2803798.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   10329..11216
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10394..10422
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(11292..11441)
                     /locus_tag="PC479_001246"
     CDS             complement(11292..11441)
                     /locus_tag="PC479_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803799.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     gene            complement(11493..12308)
                     /locus_tag="PC479_001247"
     CDS             complement(11493..12308)
                     /locus_tag="PC479_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HDF2803800.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            complement(12426..12608)
                     /locus_tag="PC479_001248"
     CDS             complement(12426..12608)
                     /locus_tag="PC479_001248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF2803801.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            complement(12795..13319)
                     /locus_tag="PC479_001249"
     CDS             complement(12795..13319)
                     /locus_tag="PC479_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803802.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
     gene            complement(14548..14709)
                     /locus_tag="PC479_001250"
     CDS             complement(14548..14709)
                     /locus_tag="PC479_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803803.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(15050..15490)
                     /locus_tag="PC479_001251"
     CDS             complement(15050..15490)
                     /locus_tag="PC479_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2803804.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(15562..16032)
                     /locus_tag="PC479_001252"
     CDS             complement(15562..16032)
                     /locus_tag="PC479_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2803805.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(16049..17359)
                     /locus_tag="PC479_001253"
     CDS             complement(16049..17359)
                     /locus_tag="PC479_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2803806.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(17360..17536)
                     /locus_tag="PC479_001254"
     CDS             complement(17360..17536)
                     /locus_tag="PC479_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803807.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            complement(17529..17966)
                     /locus_tag="PC479_001255"
     CDS             complement(17529..17966)
                     /locus_tag="PC479_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803808.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(17959..18384)
                     /locus_tag="PC479_001256"
     CDS             complement(17959..18384)
                     /locus_tag="PC479_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2803809.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(18384..18731)
                     /locus_tag="PC479_001257"
     CDS             complement(18384..18731)
                     /locus_tag="PC479_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803810.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSCKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(18725..19126)
                     /locus_tag="PC479_001258"
     CDS             complement(18725..19126)
                     /locus_tag="PC479_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803811.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            complement(19136..19369)
                     /locus_tag="PC479_001259"
     CDS             complement(19136..19369)
                     /locus_tag="PC479_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803812.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(19369..20268)
                     /locus_tag="PC479_001260"
     CDS             complement(19369..20268)
                     /locus_tag="PC479_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2803813.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(20280..20843)
                     /locus_tag="PC479_001261"
     CDS             complement(20280..20843)
                     /locus_tag="PC479_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2803814.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(20913..21161)
                     /locus_tag="PC479_001262"
     CDS             complement(20913..21161)
                     /locus_tag="PC479_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803815.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(21163..22698)
                     /locus_tag="PC479_001263"
     CDS             complement(21163..22698)
                     /locus_tag="PC479_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2803816.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            complement(22688..24118)
                     /locus_tag="PC479_001264"
     CDS             complement(22688..24118)
                     /locus_tag="PC479_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2803817.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(24133..25386)
                     /locus_tag="PC479_001265"
     CDS             complement(24133..25386)
                     /locus_tag="PC479_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2803818.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYSKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(25364..26056)
                     /locus_tag="PC479_001266"
     CDS             complement(25364..26056)
                     /locus_tag="PC479_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803819.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(26126..26635)
                     /locus_tag="PC479_001267"
     CDS             complement(26126..26635)
                     /locus_tag="PC479_001267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803820.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(26656..27630)
                     /locus_tag="PC479_001268"
     CDS             complement(26656..27630)
                     /locus_tag="PC479_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803821.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(27933..28169)
                     /locus_tag="PC479_001269"
     CDS             complement(27933..28169)
                     /locus_tag="PC479_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803822.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(28654..29133)
                     /locus_tag="PC479_001270"
     CDS             complement(28654..29133)
                     /locus_tag="PC479_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF2803823.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(29126..29263)
                     /locus_tag="PC479_001271"
     CDS             complement(29126..29263)
                     /locus_tag="PC479_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803824.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(29287..30657)
                     /locus_tag="PC479_001272"
     CDS             complement(29287..30657)
                     /locus_tag="PC479_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2803825.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(30606..30932)
                     /locus_tag="PC479_001273"
     CDS             complement(30606..30932)
                     /locus_tag="PC479_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF2803826.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(31214..33631)
                     /locus_tag="PC479_001274"
     CDS             complement(31214..33631)
                     /locus_tag="PC479_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803827.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(33643..35601)
                     /locus_tag="PC479_001275"
     CDS             complement(33643..35601)
                     /locus_tag="PC479_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF2803828.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(35601..36173)
                     /locus_tag="PC479_001276"
     CDS             complement(35601..36173)
                     /locus_tag="PC479_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF2803829.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(36196..36768)
                     /locus_tag="PC479_001277"
     CDS             complement(36196..36768)
                     /locus_tag="PC479_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF2803830.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(36821..37156)
                     /locus_tag="PC479_001278"
     CDS             complement(36821..37156)
                     /locus_tag="PC479_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803831.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(37171..38337)
                     /locus_tag="PC479_001279"
     CDS             complement(37171..38337)
                     /locus_tag="PC479_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF2803832.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(38349..38786)
                     /locus_tag="PC479_001280"
     CDS             complement(38349..38786)
                     /locus_tag="PC479_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803833.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(38857..39006)
                     /locus_tag="PC479_001281"
     CDS             complement(38857..39006)
                     /locus_tag="PC479_001281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803834.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(38987..39379)
                     /locus_tag="PC479_001282"
     CDS             complement(38987..39379)
                     /locus_tag="PC479_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2803835.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(39410..39607)
                     /locus_tag="PC479_001283"
     CDS             complement(39410..39607)
                     /locus_tag="PC479_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2803836.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            39683..40057
                     /locus_tag="PC479_001284"
     CDS             39683..40057
                     /locus_tag="PC479_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803837.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(40067..40276)
                     /locus_tag="PC479_001285"
     CDS             complement(40067..40276)
                     /locus_tag="PC479_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803838.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMSKSEKRKISEITKERIRK"
     gene            complement(40323..41138)
                     /locus_tag="PC479_001286"
     CDS             complement(40323..41138)
                     /locus_tag="PC479_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDF2803839.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            complement(41141..41362)
                     /locus_tag="PC479_001287"
     CDS             complement(41141..41362)
                     /locus_tag="PC479_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2803840.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            41515..42078
                     /locus_tag="PC479_001288"
     CDS             41515..42078
                     /locus_tag="PC479_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2803841.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            42102..43691
                     /locus_tag="PC479_001289"
     CDS             42102..43691
                     /locus_tag="PC479_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2803842.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            43954..44460
                     /locus_tag="PC479_001290"
     CDS             43954..44460
                     /locus_tag="PC479_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF2803843.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            44700..45830
                     /locus_tag="PC479_001291"
     CDS             44700..45830
                     /locus_tag="PC479_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2803844.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITVEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT        14192 a         8497 c         4975 g        18349 t
ORIGIN      
        1 aatagttact aattttataa ttctggtata aaacaagtaa tctcactatt taaaataaaa
       61 tatatttgcc taccatcaac tgaagctcca aaataactat aattaggaac agctccttta
      121 tcaaaagtta attttttaat tccactacta gaaaaaataa caactccaca tttgccactt
      181 ccagctttat aaccataatc tgatacatat aaagttgaac ccaattgtac agcaaaagta
      241 ttactatcaa agccttctcc tgcataacta taagttccac ttacagggtc gtaattaatt
      301 gttgaaaaat agtgagaaga aatagaactt tttagcccaa acacaacaat tttataagtg
      361 ctgactttag cgcaaacaaa ataatgcgat tgttttgaat ctattatagt tgattttgta
      421 gaccaagtgt ttaatgttgt attataatat tcaacaactc tgactgcttg agatgaagaa
      481 gaatcttctc cgccatacaa aacatgtatt tgatttgaat acatttcaca acaagcactt
      541 cctcttgcgg taggcatatt tgctatttca gtccaagaac tagttaaaat attatatttt
      601 tgacatatag ctgtgggagc tgaatatcca ctaaaaccac ctattttata gatatatcca
      661 tcataaaaaa atagcgctga ttctacaaag gaagaaggaa tatttttagt tgttgttgtg
      721 tttaaaacag tatcatagca aagattattt aaacctgatg tacctgtata accgtttata
      781 aagtaaatct ttttttctgc cgaaacacat ggtgttttat ctttaaccgc agtagacaaa
      841 gctttaataa atatattttt aggaacatac caaggtggaa caatcatgtt gccttgctct
      901 attttaccaa cattttcagc taacgtttta aaaggtgcat tactcacagt tgatacacct
      961 tttttactta gatttgaaac aaaagtactt cgtattgaac ttaatgtatt cttagttgtt
     1021 tctaatttgt catttcctgt gaaaggcaaa cctagaacat cagctatatt atttttgact
     1081 gtctgcaaat catctttcgt ggtttctaat gtatctatca cttctcttag ttttgcattt
     1141 tcttctaatt tcgttgccat ttttcactct cctttattaa atcatatcta ttaaattatt
     1201 cactatagtt attcctttag ttctttgacc acttatttct accattattt cctctaatac
     1261 tccatctaac ttatcacttg taaatctatc attagcatct gtaatactta tactggtatc
     1321 tataagctgt atactgctaa tagaatcctc tattttctta gacgaataag tagtcatttc
     1381 agacactctg ttatcatcca cagttgcatt aataaaatga gtttctgcat ttccatttat
     1441 cacataaacg tttaattctg accttgcttc acttctaatt tctatagaat tatcatctat
     1501 aattttaaag tttgtaacta cattttcttt tgtagtagca tctataatat ttacaactat
     1561 tctctgtgtt aataaactat gtgttacagt tgctttgaat ccactttctg catcctcaac
     1621 ccaatcatca attgcgaccc tttgagtgta tgcaacattt gagccacctg cgattaattg
     1681 gtcgatttta gtatttaact ctgttttagc agtttctatg ttgcttgtta attctgtttt
     1741 agttgtatca attttagtat taacagtacc tattttagtt tctaagtctt gtatatcttt
     1801 gagtgttgca aagattattg ttgggtcaat tttaagttct atattattta cattagatac
     1861 aataagcaca gttttaacct tcatgtctac cactgcacct tgttctatag aaggtttata
     1921 acactctttg tatttagaaa tggcaattaa attattttca tcatctaaat atcctatttc
     1981 tcttatcata aacccgccta cacttgatgg tattaaactc tctaatatta tacaatttgg
     2041 tgcagtttca tctgtagttg tatttccaat attgccttcc cataccacgt ttttgagagc
     2101 tgtctgactc tcagttggag tatattcact ccctcctcca tcaccaagtt gaatttttac
     2161 aaatcccact ttattacctg tgacacttgc atttgctatc tttgctttcc ctacatctgt
     2221 aattatagtg taataacttt tatctatagc caatatatca cctcctaaaa tattgttatc
     2281 tcttggtatc caactccatt gccagttaat acatcaattt ctccataagt ttctatatct
     2341 ggtggactcc aagggtatat agttatttct tgacctatta gggttgttat accaaaattc
     2401 atataattgt ctttacttac aagcactcta gtgtaatcta aagtcatgtt gcaaggttta
     2461 atactactta caaaagaatg aacctcctca aaccaatctt gatttctagc atcactttca
     2521 agatgtatat tataagtagc attattaata gttaattcat aattgccttc tccaactata
     2581 ctatctagcc agttccttaa aaatctctct gagtaaggta gtttacttat atatttacta
     2641 aaaatcctaa accttctatc ttctaaactc tcattacttt taggagttat agacattatc
     2701 ttttcccatc tttttatacc acttggagtt aaatcctcta aaaactggtc acttgatagg
     2761 tcatttaatt tttcatgtag tgtttttatt tctttgtttt ctacattaaa tacttttata
     2821 tattcttctt tatcttgtag aatttgtggt aagtaattta ttagattaat ctctttatcc
     2881 aactacctca cctctcacta ctatactgtt actatctatt gttagattag atttaacctc
     2941 atttatcatt gtatttgcaa tgtctaatac tccatcaata ctaagtaatc tagtttcaat
     3001 ttgagatata cggactatta agttttcttc atcttcccaa ctcatgttaa gttcatttaa
     3061 atagtcgtct attgcttctt ctgcaattga ttttatattc tcccaagtgt agccattttt
     3121 gtatgttatc tctgctgata tatttatagt tgtacttgta actccttcaa ctgtgactcg
     3181 gtgtccaatt ggtgctaatc caagaccttc tccttggttt tgtagaggat caatttcttc
     3241 ttgaactaaa ttaactaaat cactagatgg aactttgaag ttagaattaa ttattactaa
     3301 cttaacagta cctccaccgt cccacacagg ataaacctta actcctccaa catcttgtat
     3361 tttgttaact tcatctttat aattttgcat atttccacca aaactctgtg agtttaggct
     3421 atcataatat ctttgtctta aactatcttc actttcttca tcttctccat taattagtat
     3481 ttcagttaac tcagcagttt ctaatttgtc tacatattca attggaatca actgaccaag
     3541 ttcaaataca ggtccagtag tttcacattt cattttatat gttttttcag atattctctc
     3601 aattgccaca taattgtatt ctcctaagtt gaatcttgaa tcaataggaa tatctatatt
     3661 aaaaactcct tttgcaattg tattggttgc aggtaaaggt gtaatacctc tttctttaca
     3721 tctcttctct aaatagtaat aactagcagt atctacaaaa gtttggtcta gtaattcatc
     3781 catggcaatg tatgtttctg taagttctat agcaacagga gcaagagcat tatatattat
     3841 agaaccttcc cttttatcaa aagtatctgg tacactatct aacattcttt taattatatt
     3901 ttcaaatgtc attaactcaa acaattatac actcaccacc ttctctgctt ttatatttcc
     3961 atatttactg taaactgtga acttacaatg tactttaccc tttacatttt gaaactcaaa
     4021 attatctaca ttttcaaccc tatcatcttg aattagtgct tctttgattc gtctttcaag
     4081 ttcggggatt acaaaggata taggctctcc aataaggtcg ttcaactcga ctccataatt
     4141 ccaactatat attagatgtt ggtatctctc tgtgtttaaa attaaaaaga tggtttgttt
     4201 taatgcttca acatcatcac aaataccatc tatcttagat ttttctatat ttaatttaaa
     4261 ggtcttactt ggttcttgcc ttacatcaaa attaattatt gatacatctt caatgtcata
     4321 atctaaatta tcgcttggta acacttcatc acatcctatc caatactaaa tattgttgtc
     4381 ctccttgcat acgaattaag actactttat ctcctatttt tttatctgta tatcttttaa
     4441 atgtatctgt ttgtattaga aaaatttcac caatagatag tttttgttct atcttaactc
     4501 ttagaggatt aatactttct attgttccaa atacaaccct cattgggttg cttgtttcta
     4561 ctgcatccat tgcagctttt tttattatct gtaataaatc ttggctcata ttgtcacctc
     4621 acttatataa atcttctcac atgtgtgtat gcttttcctt ttctataaga attaacagac
     4681 tctattttta ccacatctcc tgtttgtggt gaatgaatta tttgattgtt tccaatatac
     4741 attacaacat gattactact tcctccacca attctacata ataagtctcc tgctttccac
     4801 ttgcttctat cttttaaatc tactgctttt cctgctttac tttgtgcaga agcggtacga
     4861 ggaattttta tacctatttg tttataacac cattgagtaa acccactgca atcaaaatta
     4921 ttaggacctt ctgctccata cacataatta caacccagtt tactttttgc tatactaatt
     4981 aatttatctt ctttagagtt attatttgta ctactttggt tattaccttc aacttgatat
     5041 gtttgttctt catctccacc tataattata tagccattct ttctaccaaa ttttttacat
     5101 tcactagcat tagctaatag tatatctata tgatatgttc cgtttgtttc aacatatatt
     5161 cttcctccat tatctttaac tgtatatact ttgttgtcat aggcagtacc aggaagtata
     5221 atttttactt tatctccata ttcaaaaact ggatgtttct ttagaaaatc atcagtatac
     5281 caagttttct taactccttc tcgattcatt ggaccagcaa cagttcttga ttttacatca
     5341 agtggctttc cattgcaatc tgtttttccg ccttccattg cattgttccc tggataatat
     5401 gcagtaaata tagcaggaac ttttttacct gtattttttt tcgtaatact ttgtgcagga
     5461 ccatttttct tttcatcttt attattagta tttccactac tatatgagct tgaagaatat
     5521 gaagcaaatt cgtctccatc aacaagggtc aaatccataa agtgcgaatt attttcaaat
     5581 gtatgtttta ctttctcaac taacatataa ttttgtaatt caatatctcc caaatctaaa
     5641 aaaacaggta ctaaacaacc tgctctcact ctaatatcac caagtacatt ttttaaactt
     5701 aatgacttag ttttcttatt atatagtttt agaagtatat cacacttttg ttttatctct
     5761 gcttcactca tgtttttgtc tactgtatca aacatttgaa gtattcccca actcctcata
     5821 tgcgttgagt cttgagcaat atacacatct ctttttcctg tttcttcatt atctctcaca
     5881 agtttaatct ttgtgtaagt atcactatct attgatgaat tatagtcaaa atcttctatt
     5941 acatcattgt tcataacagt atctaatttc attgatgcaa cattctttaa tgttattctt
     6001 ccaaaatcat catataacac atacatttct tttttctctc ttagagtatc atctagtgct
     6061 gttagtatca tgtcaaagag tgttttattt tcttcgactc tagatagttt atacttagta
     6121 tcttctatga cattgtattt taaattaaaa tctttagcca acatttttac aagttcactt
     6181 gctgttttat tgctatacac ataagtatct ttgttcttaa aatatcttaa ctggtcgtaa
     6241 gcaacaattt taatgtgatt ttctttatct cttttcttct gaaatatata tccatagaat
     6301 atacctacac ctttgtaata tagtcttaca gagttacctt cacaaaactc taatatatca
     6361 tccatgacta ttgtaaattc aagcttagat ggtgttcctc gtctctctat ttcccatgtg
     6421 ataccatcaa tgacagcagg ttcatagaaa tcttcccaat gtgctatgat taatcttaca
     6481 tctctatcat tcgccagaac taaatcatca gccaagtttt aacacctgcc ctttgtaaat
     6541 agtgtattta cttaagtttt tgcccttatt tgccttatcc atcatagatt tatttagttc
     6601 gtatactttc ttatataatg aaccattacc aagttgcttc ttgcagatta accaaaggct
     6661 atcccctgct tttactgtat atgttttacc atttggctta ttggatgaat ctggtctaaa
     6721 ttcttttggt ttcatgactg gagggggagt cctaccatag tttgtctttt caggagttgc
     6781 aggaactaac tttttagttg agtaatctct atattgcttt aactttattg caacttttgt
     6841 atctgagcca ttttctgcat cttctactat gttatattcc tcaagagata ctttcatatt
     6901 agtgttaaat agtactttat tacctaattc cctcgataca ataaattgaa atggcttaca
     6961 atcagttttt agtaattcta gtttacttaa aaagaattga acatctctaa attgacctct
     7021 acaaaaaggt aatttattat gtgtaaattc tgcttcaaaa cttatttcag ataatccttc
     7081 tttttttaat atatttactt ctccaacatt tattaaatca actgtcttgt ttttatttgt
     7141 aactttaatc tctaactttg gcggaggtat tggtaattgt actccatcta aataaaagtc
     7201 ataagccatt tatatccctc ctctctaaac tattccctca gctgatacaa ccatagcgtc
     7261 gtttaatttt tctgttagta catttactat tccatctaca tctgcctcac tatttatgtt
     7321 gtttgtattg ttcatgtcaa ttttaatgtt tactcctgtg aatctattta ttgtttcttg
     7381 ctctgcaata tctctaagat attttaagtc ttcttgactt ttatccattg ttttagccat
     7441 tttggcagta tttcccgccg tgtcctttac attttttcct aaatctccac caaatcctcc
     7501 aagattatta agtggattgt ctttatccca caaatctttt aatcctagct tatcttttgc
     7561 atcttctagc atcttattaa tgtcaaaagt atcttttaat ttattagata tagcattttg
     7621 ccatttagtt ccaagtgcat ttcctttttg aaattctgct ccaatatctt tgtatcccat
     7681 tcgctccaat tttactttct ctggtgcgtc tcctacccat ctgtttaagc tatcaatctg
     7741 ttgtttgatg taactattat ctgccttaac tggcgtaaac gttgcctctc ctactttacc
     7801 aatatttatc cctggtattt tatttattag gtcaattaac ttatttaccc ctcttattgc
     7861 tatatttgct ccatccacaa aagccttacc aagtgcgttt cctgcattat ttacagaatc
     7921 attcaatgat gccattttct caattataaa aattacacct tttgcaattg cttgtttcat
     7981 caaatataca cactgattcc atccattcgc aattccttca tttacatcta cacatctatt
     8041 aaatagccat atcattatat tttgtattgt tgcaactgct gcaaatactg cacctacaat
     8101 cacccctaga acagtcaaag acgtacctgc aaatttattt attgccgcta ctaccataaa
     8161 tattacagct attactgcta taaatccaag aactatccac acgagcgggc aagcatacaa
     8221 cgctgcattt aggccatatt gtgctgctgt tgcctgggct gttaccatcg cttctatagt
     8281 ttgtgcagat attttaccaa ataaagctaa ttcttgtgct gccaatgctg cacaagcttt
     8341 atatgcccct aaagcataca gggcatttac aaaagatgat actccagtta caattgcact
     8401 tgcaagtaaa attcctttat atattgcaat agctgcgaca actccataaa cagcaggtgc
     8461 aataatactc caattttgag caaagacatt agcaacattt agtgcagcat tagtaatcca
     8521 tcccaatcct tgtattatca gtacagttcc tgttgtaatt ccttgtacaa aatctgaaaa
     8581 aaaatcacta tttaatatat tttttataat atttaatgta tcataaatgt ttgaagatat
     8641 tgcaatcata acattccgca tagatacaac aatctcacta aaacgtacac tttgcaaagc
     8701 tccgcttatc ttactgagag tctcagaaaa tatcatataa gcatcatttt tcatcattgt
     8761 gagagcttga gaaaaagtga ttggcatact tgcaaatttc ttttctatct catcagaagc
     8821 tttaaacagt gcatttctta taacatctgc tgttattgct ccgctacttg ataattcctt
     8881 caattggtct ttagtttttc ccattgcatc agctatttta gtggctaata agggtgcatt
     8941 ttccattatg gaacggaact catcaccttg aagttttcct gcacccatgg cttgtgttaa
     9001 ttgatacatt gctgcactag cttcatttgc tgatgttcct ccaattacaa aagctttatt
     9061 cataagttct gtaaacttta tcacctctgc tgaacttcca aaagcatctc ctgcaagtat
     9121 tccaagttta gcaatctgtg ctgctgtatc tgcataacta gctcttgcac tttgggctga
     9181 caaataaatc attttattaa gctggtctgt tgtctgtaag ccatcattca ttagatttaa
     9241 acgtgctttt gtacttgcaa ttgtatctgc tgcttttgta atactttcta ttccttttat
     9301 tccaatgtat atcccagcaa tactttttaa tttagacact aatagagaac ctgcttcact
     9361 accttgcctt attttattat taaagttttc ctgctcattt gtatttcttc caattttctg
     9421 ttctatcctt gtgagaatac tttctatgtt attcaaactt tgttgagaag cttgtatatt
     9481 acctgcattg agtggattat tcaatctgcc ttgcaatctc tctaaactat taattgttgt
     9541 attaatagaa ttagtcatat tacgaaatgc tggtgtcatt ccgtcgaaaa tctttattga
     9601 agtttgtatt gtagccattt cctcactctc ctttcataaa tttccatata aaaaaacacc
     9661 tacattagta agtgtttatt atttatgccc aaaactgttt accacagctc aaacatgtaa
     9721 ctctaacttt ctttgctcct aaatttccag ctactaaacc aatgcctcct gctatagtag
     9781 cccctgctac agcttttcct atgccaaaac ctttcttatg agcagacaat gaaacagagc
     9841 cacacctagg gcaacgagca acatcatctt caagtacatt agtttgttta tactcctctt
     9901 ccctcaatat ctctttttcc tcttcgatat ttattttttc acttaattct ttaaaagctg
     9961 gatatataat atttttacac tcttttatgt ccagccctag taacttcatt gcaatatcaa
    10021 ttgctaagat tttatctcct ttagtttctt tataaatatt agtcagattt aattcttttc
    10081 catgaacatt tataaataaa tcatcattgt taactttatc tgctaatgta gctgttccgc
    10141 aatttgagca aaatttgcct gcaccagtta tctcataacc acaatttgaa caaaacacaa
    10201 caatcacctc acataatact tattttatag cactattata ctatatacgt aaaattttta
    10261 cattattatc acctcctttc ataaaaaaaa acacttactc atttgtaagt gtttttgaat
    10321 tatttttaat tttaagtcca catagttaat ataaaacgag catcatttat cattgctaat
    10381 cgtgcatttg tacatttaca taccacatag ttaatataaa accatacact agattctacc
    10441 ccaaaaatga agctcaatat atttacatac cacttagtta atataaaacg cgttaatgca
    10501 gtaccataac gatatgggac taactaattt acataccaca tagttaatat aaaaccgtat
    10561 aggaatgaat gcaggtagtg cattttctag tacatttaca taccacatag ttaatataaa
    10621 acgcaacccc taaagacctt ctaaaaaaat tactcttaga tttacatacc acatagttaa
    10681 tataaaacac aggtgtattt acctatagaa caacaagagt tacatttaca taccacttag
    10741 ttaatataaa acattcaaga agtaactgta attgttcatc tgtaaaataa tttacattcc
    10801 atatagttaa tctaaaacct ggtcttaaaa acatacatgt tgcaaaagtg aatgatgaat
    10861 ttacattcca tatagttaat ctaaaacgaa cactaacaaa aaagctaaaa gtggtcacta
    10921 tattatttac attccatata gttaatctaa aacgaatgat acattagaat atatattttg
    10981 tagtgaatga tttacatacc acttagttaa tataaaacca tcctacttag atactgcaca
    11041 aatagtatcg cgaatcttta cataccactt agttaatata aaattgacca gtacgaatgc
    11101 gaggctagca atgataaacg actttacata ccacattgtt aatataaaaa ccttgagtga
    11161 cgcagcaaaa ggggcaaagg atacagcatt tacataccac ttagttaata taactcctac
    11221 ctatattata tcatttttta ctacataaag cacttgaaac agtaaaatat tcaagtgctt
    11281 tatttgtcta tttatttttc tctttttttc ctacattctt ctttcactaa acttacaaat
    11341 ctataaaatt catctggatt ttcatttttc attttttcaa agatatttcc aagttctttg
    11401 ataagttcta ttctatctat atcaagtaaa ttctcatgca ttgtattaat ccccccaaaa
    11461 ctaaactaaa atatttaata caactgataa atttactcaa ttttataaac cacatgataa
    11521 ttcttcttct cacctgcaat cttggtaggt ctattattct cctctatcca atttcttatt
    11581 ttatctatta cactttctgt atacttattt acagtaccag tccaacttcc attagtctcc
    11641 caaactccct taacttcact atcttctaaa tcaatctttt taataatctc acaaacagcc
    11701 atctgagctg gtttattact cttagaatat agttttaact tagatgctat ttgttttgtg
    11761 tcaaaataat gttcttcttc gtttatctct attggcaaat caattcctgc ctttttatat
    11821 aatgttttag ctgtaagtaa cttagattta ttgtcaaaac ctgcaccatc caatagttct
    11881 tttaacatag atgtgctgtt ataagccagt tgtaattttt caatctcact tgctttttct
    11941 cttagttttt cggggtcagc attatttgtt atgtatgcac cagtttgtcg aatggctgga
    12001 agtacttcat cacttatcca atcttgaaat ctctcagctt cttctttttt agatttaaat
    12061 ataagtttat aaacaccact ttcagttaga aaattttctc ctgtattatg caattttcta
    12121 aactgcatat tatgcatttt agaattagtc aacttaacta cttgattatc attaaatttt
    12181 ctaatactac tgttcacatc tgaaatatct aaacactctg ccacatgctt tggattaaat
    12241 aaaatttgcc cttcaaattc gaatacctct atatcttttc cttcaaaatt cattaattca
    12301 ttcataataa attcctcctt aaatttgatt gtaagaagta ccttactatg atagaatata
    12361 tttcatagaa ggtaacttct ttgggaaaca gtcgcaagtg ctttggtcgg tgcagcggct
    12421 gttttttatt tgtttttgtc aatcttctct ttcaccaatt caattcctct aattacaaca
    12481 tctgttttag atattttaag attttcagca cactcattta atatatctgc ttgttcttgg
    12541 ttaagtctaa cttcaaatct taattttttg gaattttctt ttggtggtct gcctaattta
    12601 ttggacatct tatcacctct ctttttattg tccgtactta aattataata tagtacgtac
    12661 aataattcaa gagtttttta ctaatttttt ctaattattt tactcaaccg accaattttg
    12721 agcaaaacaa aagcacctac caaaaagtaa gtgctttcta tataacagat ttaattttaa
    12781 aataaagtat tcacctaatt tccagacgag aaatccaaat catcaattaa atttacatta
    12841 ttttttatat tattccaatc atctaaaaat acattataag tgagtgtcac atcaacaata
    12901 aagcttattc tcactgaatg gatatcattc atatctttta tattgtttac aaattcttta
    12961 gtaaagtttc tacactcatt ttcatcatta aacctagaat tttccacttg aatactaact
    13021 atatatccct caccttttgt tgggtttaat atgttaacat aatatgttgt tttatctttt
    13081 aagtcttctg gtatcataga gtctactttt gcttgcatct cttgtttata aatatcttct
    13141 ttttctcttt cgctaattat attttctttt gtaatatttc cttcatttac ttcatctcta
    13201 ttttttgaat ctcgtatcgc ttgatgaatc atcattgata ctgtaaatac aacaaagtat
    13261 attaaaaata tcactaaaat tttcttcaac aaacttaatt ttttaaattt tccccacata
    13321 atgaactctc ccctattaaa ttctttattc aaatataatt ctatctaaaa ataagaactt
    13381 agtctattga tttaaaatat tttgagcaaa ataaaagcac ctaccaaaaa taagtgcttc
    13441 tttcttctat ttagtttttc tccacattgt taatataaaa gtttggagtt ttaaaattat
    13501 cattcctatc ttttgcttta tttacatacc acatagttaa tataaaattg aggtagaaaa
    13561 tgataaaaag acttttagag taatctttac ataccacata gttaatataa aacctgttaa
    13621 tgtaatcatt tcaccatcat aagttgcgaa atttacattc catatagtta atctaaaaca
    13681 ataattggtg atggtacagg atatacaaat gcaacattta cattccatat agttaatcta
    13741 aaacagaaaa ttcattgtac aattcttctt tgtatttttt aatttacatt ccatatagtt
    13801 aatctaaaac cccaaaataa acttagtatt tccaatatct acacatacac acctctctca
    13861 aatttgcagt gaaccatgag tagtgcaatt gataacattt atcacacacc ctcaatacct
    13921 tgtattccaa ttgttaaact tcaatttatc gcaaatattg ctcactgcaa aatctctaca
    13981 tttttattat atcataaaaa tattattttt gaatatctgt accaatttgt ggtataataa
    14041 aagcaaggaa ataatttact ttatacaaga gtagctattt ccatcaaaat tgatttaaag
    14101 aattattttt ttaaatcacc cttattggcg tctgggtgat tttttatttt gtcataaata
    14161 taagctgata taacaccagc tagtatgctt aataaaaaaa ctatcatata atttcacctc
    14221 cttccttatt tggaatttgg cgtttaatat gaaaataatc acccttcgca ctttcgatta
    14281 ttatccttgc tacaattatt ataacatata attattacat atttttccat tttttttata
    14341 taaacaatga aattcaagta aataaatacc tacttattta tatatatttt ataaattaat
    14401 tgctttataa tcaagttttc aattttttaa taaaaatttt tattttttat tttatataaa
    14461 caatattttt tctaatttgt ggtataataa aagcaagaag aactacaatc tattttgcgg
    14521 tagagtgaag ttcataatta aatgaatcta tttgaactta tggaacttga ttttaaaatc
    14581 aaattcccag ccacttttac tcttgccacg agttgagtgg ctttttactt ttagaaatac
    14641 tttacaaatt aagcaaaata ttaaactagc aataacgcca gctattacat taagtaaaaa
    14701 gttattcata cttcccacct cctttcatta ggaagtaggt tttatctcag tatgaactcc
    14761 actctataaa ttgtagatta catcttcttg ctaaaaatat tataacatat aattattaca
    14821 tattttacct attctatatt tattttttta ttttgctatc ttcttcgtcc cctctttctc
    14881 tctctttcag cttctttcat tgcttcctct tcatcttcta tctttataag tattgaggcg
    14941 gctgctaacg ctctctcatt aacttctaaa ttcatatatt cacttggctt ccactttaat
    15001 ttttgaatac aataatgagt gatgctagca tcaaaatcgc cacctctgat tagttttttg
    15061 cttcttctac tttatcctca aaagatgtat caaatccatt gacttcattc acttttactg
    15121 tataattgac atactcacct gctgtaagca ttgtctttaa taactgagct tctcccatta
    15181 ctccataact attttggagt tcggcatcct ttaaatctgg aaatactgta gatgctacac
    15241 ataattcagc tacataacta ttgtagtcaa tttcacttgt atattgtcca gtatgcttac
    15301 cattgttacc aatcactttt actcttttag tacactttct tcttagtgct tcgtcttctt
    15361 cagatgataa aacttttaat tcccattcaa ctggtttccc ttctttatct aaaaatctgt
    15421 tactcgctac atattttaca ttatcaacct ttattgcatt ttgacttaaa aaagcactta
    15481 aattactcat attattctaa tctcctttta ttttaatttt tcatataaaa aatacacata
    15541 tataatttat aaatgtgtat tttactccat tcctgccaat aaattaaatt tttctactaa
    15601 ttcccaatcc tcaaaagtga aatccatatc ttcatctaaa tactcaccat cagcatcaaa
    15661 tttagtaatt attccactgt ccatattaca atctttaagt actactgtct gtcttcctac
    15721 agcagatgta gggtcttcat ttgtaacttg tatgtcaaaa taaatatcct caccagtttc
    15781 tttatatctg taaagtaatt ctctaaaaat agaagtatta taatgaaatg ttgcacttcc
    15841 agtatttgta ctcccagttg ttttatttcc ctttgttgtt cttcctagaa ttggaacttc
    15901 acttttattt ttttccattt tagcctctaa atctatagct tgcataaaat tatatctttt
    15961 gccttctata gttataaaac attcagcttt ctttgcacta actgtatctt tagcatttat
    16021 tgtttgagcc atattatcac tcccctctct aactaactga aactgtcata taaagcttac
    16081 tcatagcatt tataacctta acagcatcag atactatgac agttttctta tcatttccaa
    16141 gctctacact aacatcatca gttttaaaat cttctattgc ccttatattc tctaattctt
    16201 tatggtgttt aacaacatca ttccagaaac ttattcttcc tgccttatca ttcggaactt
    16261 tacctaaata cttttcatta aataaagttg caatatcatt agcaatttgg tcaagtactc
    16321 taacactttg gttacttgaa aaatcgtcat ttttatcatc tgtaaatgat acaaaagtat
    16381 ttatatcctc taacacatga acttcatctc ccaccttgtg gaatataaat ttaccagttt
    16441 ttaaagcttc ttcaagttgt atttgtgtat aattaacatc aacatcaaac tcaccatcat
    16501 actttttatt agtattagat ttatttatat cgcatcctgc tatagctcca gccgcccaat
    16561 aaattaaact agattcaact aaatctttat ctttaatctt attttctaca gacactacac
    16621 cttcataatc tgcatcactt ttcttatata gtactgtttg aaacttagct cctaccttat
    16681 ctctcattct ctttgtaaat tctacaaata aacttttaat ttctgttgtt gtagccaaac
    16741 atcctaaagc attaaagcta tagctttcta ttttatccaa gaaagcttgg tactctgctc
    16801 ctgtcacagc ttcgccatta gttccaccag taaatacaag tcctgcactt gcttctagtg
    16861 ttgcatcctt cttccaagtg atatagtcat tgtcttgtaa gtctgtaata acctttgcta
    16921 tttgagtatc taccttctta ttatctaaaa gtgttacaac atcaaattta gtgttatcat
    16981 ctatatttgt tgtaactgtt acttttaagt catttcctct gataccacta tattttgctg
    17041 tggctatagt acaactggct ttaacgcctt tatttaattt ataaaaatat cccaacctta
    17101 tatttttgaa taaatctctc aaacctttca gcttctcatg agtataatca tatccaaaat
    17161 acttcactga atacttctca aaatcatcac tggttacttg aaatacgtct ttatctatgc
    17221 cccaatctaa ctctaaaggt attgcaacaa tacctctatc cgataatgaa ctggttgccc
    17281 ttgtagcact tacaaaattt atatatgcac caggtaggac cttattttgt gttacaaatg
    17341 ttcctccacc taaagccatc taactcactc ctttcataaa tttatttatt ctatcctcta
    17401 cctctgagaa ggaatataaa tcattttctt ttaaaattgc atttaataaa tcttttctat
    17461 ttacatactt cttagagtta actatctgct ccttagtaaa cttgtagtta tcttctttgc
    17521 ttaatgtttt actcaaaatt atcacctctc ttcaaaccac cgaataactc tactgtatcc
    17581 atcttatctg tgtcattact ttttatagta aagtagttat aatcaacaaa gaaatgaaga
    17641 acattatcta taatttcaaa gttcatattt gtacctctga ctaagtctcc atcaatttct
    17701 atatactcta attcctccag taacatctca gctatctcat ttatttcaaa tgatttatca
    17761 ttacttttag gaaaataatg tacatcaaaa gagttctttt ttaatgtcct gccacttaga
    17821 taggatactt tgcttggatt taaaggaaca ataaaaaaac aaggttcatt tataccttgc
    17881 tccacatctt cactataaat tgtataactc tctccaaatg ttttatctaa tttaatagat
    17941 attccatcaa ttatattatt aagcatcaaa tactccttta agcaatatta atagtttttt
    18001 ctctataatc ttatcaactt ggctttgtag ttccatctct gaaattgtta agaaatgttg
    18061 tcctttaacc caaccttttc catctttagt tctatggcca tattcaacat aacttgcata
    18121 tgtggtcgga ttaacaacct ctataatata attatttcct tgtttataca caggaagcga
    18181 cctagcataa gccactccat tccatccttg tcgtaagaat cctgtatcaa ctggtgttct
    18241 tctaattact ttcccaagta atcgtgctgc taattctctt gctgcatcct tgcaaaactt
    18301 atctaaatca atctttgtaa gctcctccat ctttttacaa actcttttaa actctctaaa
    18361 atcaacactg ccccatctag ccattatgct ttatccttaa ataactcaag tattatttct
    18421 tgatgatttg gatatatagc tgattctcca cttcttacat actctttatc atttataata
    18481 agttttgaac ctgctttaat ttctatatct ggagatataa agagtttaat agtttgctct
    18541 agcttagcta ctttcccttc tgtagcagaa actatatttt tacatgaaag tttacatggt
    18601 tgattttcta atacaatcac ttctttattg ttagttcgtt ttgttacagg gtctttaatt
    18661 ggctgatact caactatagt acatttatct ctatataaca tttctattgc ttttctagtt
    18721 ttacttacca tcttaagcac ctaaaggtta atatcttatt cttaccataa gtagtaagat
    18781 aagctactaa gctatcaaag cgttgttctg gtgtttgaga gccacttcct atagcaaaat
    18841 ctacctttgt atcaccttct gatatagact tttctacagc ttcaaagtta atgctttcta
    18901 tatctaattg ccccatattt ttcttggtaa ataagaactc tccaactatc atatcaactt
    18961 caatttcttt caattcaatt ggcatagttt ttatattaca atctagttta ataatatttt
    19021 ctattttttc tcttacaaaa tctattaacc acttatcccc atcttttaat atatatccaa
    19081 aactttcaag tcttttttct atatcatcaa ttatattatt tcccataatt ttcacctact
    19141 ttttagtaag tttatttttc tctttaagct gtttattttc ttcttctaaa gactcaactt
    19201 ttgaccttaa aatattattt tcagctatta aatcttttac atttaatgac ttgccatact
    19261 ttactgcctt accagtttca tctatcaaat catatcccat ctctaagaaa tcatctattt
    19321 tacattcttc tatagttaat attctattta atttccttac ttgtgccatt atgctccagc
    19381 tccttcaaca acaaattgta ttgcatcagc ttttttattt aatataaata catcctcaaa
    19441 actttcttca aagtagaagt attttccctc tgtaactgct gttggttcgt ctaacttaga
    19501 gaactgataa gaaacaggtg taattattgc acttgggtga actaaggaca taaagatttg
    19561 tttagctcct gctcctactt tccatccagt tgtaaaatca tatgcagttt tcattagatt
    19621 agatggtact ttaattattt taactgtgtc aatatcagtt gtttgacgat taagagaagt
    19681 tcctgcatcc tttatattta ctgttctttg tatctctttt gcatttttga taagtgtatt
    19741 tactactgga gtaacataca atattcttcc attttcaggt actctagctt ctgtcatttt
    19801 ttccattaac ttatcaaata cttctaatac gtttgttgtt gtaagaacag ttgtatctgc
    19861 tgtgttacct aatgcggtcc aatcagcata tattttagat atacagtaag catccatctc
    19921 tggaaacttt tgttcctcat tatatacttt tgttatattg cctattgaag ccacataatt
    19981 agtttggttt atatctgctg gatgaaccaa tgttgaccat ttcctttgat tagttaatac
    20041 cttaggttcc caagcattat catagtttct ttgagctact gctattgtat ctctgtttga
    20101 atctactctt ccagttgtag atatagttgg tatttctatt gttttagaac cagtccatct
    20161 atatcttcca ttatttggtg ttgcatacaa atccccgaag tttaaagtat aaggatatgc
    20221 ttgtgctaaa acatttgaat attcttttgc ataatttagt gctgccattt tatttcctcc
    20281 tatttattat tattttcatg aggtcttacc ccagtaaaat taaaaccaaa atcatttatc
    20341 ttaggctctt gccctggtgt tatagtatct attttaggct cttcaccttc tagtgttgca
    20401 ttaaacaaat aatctttatc ctgtttcaaa gggtttattt gctcttcaaa agctttttgt
    20461 ctatctttac tatttcttag tgcttctata tctaaatgag cttttaacgc tatttcatcc
    20521 ctacatttaa tagacttaaa agcatcattt aaccaataat taaagtcctt ttcttcaatt
    20581 tcttttttgt aggtttcttc caaagttttc ttatcagttt cataagttgt ttttagattc
    20641 tctacatctt cttttgtcat acctccttca aactttttaa tagcttcatt agctgtatta
    20701 agttgtgttt caagatttgt ataatcttct tgagtaattg tagtctcttt tattttcttt
    20761 tctatagatt tttgaagaga aactacatca attttgttat tttctatttt tattccttct
    20821 agcaattctt ttaaccaatc catcttttaa atatctcctt tcatttttta cataataaaa
    20881 gcacctacta atttatcatt tagcaagtgc ttttacatat ttactatttg tatatctttc
    20941 cataaatcct ttagtaattt accatcaatg ttgtaattat caaccatatc cttaccattt
    21001 ttataatact ttgtatctcc attagggcaa agagtaataa accttgtatc atcatctcca
    21061 atagatatat tatatggttt attatataag tcaaattcta tatctaatcc taaatcaata
    21121 gaatcaatta aatgttgtaa attcttaaat ttattatcca ttttactctc ctttcaaaat
    21181 atctttgtta gctattttat gagcttttgt aagctccata tccttctctc tttttacctt
    21241 atcatggtta ttttcatcag ctaaccaatc atgtttatga ggtacaattt tatgttgctt
    21301 tgggtttcta tggtcagtta agtctaaatc taatctaggt tttcctgtat taccatagta
    21361 tcttctttga attaatttac catctttgta attatcaaat acactatttg gttttgattc
    21421 aaatggcact gaatgaacac ttccactagt taaatttctc tgattcttaa cttgccaatt
    21481 tacatcctta taaagctttt tagcttcctc ataccttata gtatcattat acttcatatg
    21541 ttgatattca tcaaatttag aaggtatttc atttcctaat acctttttat attcttcaaa
    21601 ttgttttcta tctttactct catttagttg catttttcta agggtatcag ctttttcttt
    21661 tccaagtcta ccctctatat gtttctcata ccactcatta tacttcatat tagatggtac
    21721 ataatatgtt tttccatctt ctccttttgc tgctctgtaa ccttcttcat cttcaaacca
    21781 aggagctgtt gttgtcctac aacgacaatg aaatggtgga gctgttattc caacttgata
    21841 atctttcata tcaaatactt taccatctaa ttctctgcat atatttgaag ttcttaaatc
    21901 tagtgtagca attatctcat atttctctac atctaaatca ttaaaacaat cttttctact
    21961 tgctgatgca aagaaagctg attcagtcat tattaagttc ttagcttgag atttagatac
    22021 attaaatctt ttagcaaagt catttactag attctttgga ttttcacctc taataattga
    22081 ttgagttagc ttagtatgta gttcatttac taaagcatgt ctatgcttac cccaaattct
    22141 ttcactaaag tttaatccat ctgttgccca tggtttagag ataattttat ttattctatt
    22201 agtatcaaga ctcattaaac tccaaccaac gtttactcct tgttgaacat taaaagctgt
    22261 atgatagtat ccacttgtat aaatatctct cattagttta tcaataccat caagttcatt
    22321 tccatataaa acttctactt gttgctgtat ttgtaacttt aaagcttcaa gccttgttat
    22381 atgaactctt gcactagcat tttctaactc tttcatccac ttttgattta tagcattttc
    22441 tttaccatat ttaatatatt cttctacact ccatttaaac tcttctagtt ctcttgtatt
    22501 tagtagtttc ttagcttcta ataaagatat tccttcattt ttggcaaatc tgttgtacca
    22561 tgctaatata tctttttcta tattatttat agctagttta tattgctttt ctaattcaag
    22621 ataatatttt acacttttgt tattttgagc ttcttctaat tgttcaaatc tctttctcca
    22681 ataatcttta tgtttcatct ataacaccat cttgattatt aggaattaaa tcatcatact
    22741 ctttttgagt atcttcctgt tttttaagtc tctcaagttc gtcattaaca tcctcaaccc
    22801 aaggatgatt ggaaacaata gtttcatctg atatgattcc aattgattta gctgccatat
    22861 ctattttttc agactcattt attatcattg agtggttaaa agtaatttga actgatttat
    22921 aatcataact tttactacca cttatcttta aatactcaca tacaaaccac aaaagttctc
    22981 taattgcttt tttaaacttc ttttcagttt tggagcattt taaatcaagt agtgaatata
    23041 aaaatttaag tgctacacca gatttgtcac ctgtgttttg agattctgga ttaactcctt
    23101 gaccaaagat aattatattc ttttccaatc tatcaagaag ctccttttta gcttcaactg
    23161 gtatatttat ctctagttta tcaactccac ctccaccatc tactttaatt gatttatagt
    23221 atcttatatt atctataaac tcttgtagac ttgttcctgg atattctttt aatacataaa
    23281 taacctcttg tatttcatct aagttatctg ctagtgtaga aatattattg tcatatatat
    23341 ctattaatga tttatagaaa tttaaatctg atacgcactt ttcattattt ttaaaaggta
    23401 taaatggaac tttaccccat ccctgttctt tgttatttat tctaaaatga ccttcttgta
    23461 tatcagtcat ttttccatat tcatcatata aaaattcttg aataaaacta ttacctcttt
    23521 caataaagta agttacgtca ttttctgtgt agtactcaac tctttttatt ttatttccat
    23581 ctatatcttc aatataataa aacctaataa atgcaactaa ttccctctgt cttttactat
    23641 cccaaatagg aattgcttct tcagcaggta ttattacata tttaaactca ccttttctat
    23701 taatatatgg atgtaaccat tcaatacctt tgttactagc attgaggtag agttctgtta
    23761 ttgtatcgtc aaattcttct cctagtaagt catttaaaag cttagtgaga ttatcatcat
    23821 ctgcattaaa tactatggga tttccgacac tatagcctac cttttggtca actaaaagct
    23881 tatggtagtt gttaattgct ttattattaa ctttagtaaa atcatcaacc ttagctccat
    23941 ctaagagata atatcttctc ttattgttta tatctgcatt gccataatag tattcttcac
    24001 cttgtttata tttctctggt ctatgtttta aaatataatg ctctataact tttactaggt
    24061 taaaggtgct ctcttttttt aactgaactt ttattaaatc tgtttcactt atataaatat
    24121 ttaacgcctc ctttacttta agaagcttat tccattattt ttaagcttat tatctataga
    24181 atatctaagc gcagccattg catcatccat aaactcaact ggttcatcaa gatataatcc
    24241 agttctttca tcttgtttcc atttccattg ttgtatttct tttatggtat tagtgcaact
    24301 aggatgtaca tgtattctta attgtttcaa ataatctatt tgagctttaa cacttcctgg
    24361 tcctttttta actcctttag cttcatatcc tgcactcttc cacatcttaa ttctatctgg
    24421 ttcagcacta tcacagtaca taaatagatt cttttctaaa cctttattat ttgcaatctt
    24481 tatgatttct gaggtatcca tttcatgtac atatatttcg ttacatatat ataattctcc
    24541 atccttaaag ccaattctaa gtactacatt tgcatggtta aatccaaagt cttgtgataa
    24601 cctcatattg tcaaaatact caaattctgt aggaaattca tgtataacat aatttttaag
    24661 tattgctcca ccagtttctc cccattctcc aagaccatag actttgtacc cttctgggtc
    24721 ttgctctttt ctcatttgca ttcttctgta gtaagcctca tctatgaatc tattttgtag
    24781 ataagtacta tgatgagtaa atatatcatc atttttatag tcaaaatact ttctttttat
    24841 ccaatgagta gctgagactg gattaaatgt aaatgtcatt tgatagtata ggttaggatt
    24901 agttaaaata cctcttaaac ggtcatctag tatgtctatg tcactttcca taagttctgt
    24961 agcttcttca caccaaaccc atgttaattt tcctttcgag aagttaattg attttaattt
    25021 ttctctttgt tttgcatcat taactcctct gaaaattata gagttaccag taactttact
    25081 cttaatttct aaaggattta aagtagtttt ccaatactta tcagcttgtt tactataaat
    25141 acgatttata gctcctgtaa gctctgcata cgttgaatac ttatgtgtag cttctgactt
    25201 tctaactact aatagattag ctccttgata cttcttatct cctaacttta gtatatagtc
    25261 ttgtgctaca ttaacagatt ttccactccc tgctgaacct ttcattgctc tgtatctttt
    25321 tttagtaaaa ttagcttcct tgaaatctgg attaaaattt actctaacta tcatttctat
    25381 caccataatc tacacttatt ttcaactcat catctccaat atcatcttta cttaggttat
    25441 caacttcaca tttcaacttc tcaactcttg ttttctgctc ctctgtagcc aaattccaat
    25501 ccttatgaat catttcatca tactgtttaa ttaaacttct aagttcactc atagctctac
    25561 tctgtgcatt aagaaaagat gcttgcctat cccatgcaaa ttgaaattca tactctatct
    25621 tttcaccatt ttctgtgctt tcatatttct ttaattcttt aaccatttct tccttgcctt
    25681 taacatacat tatcttttgt gctcttatta ttgctgcata ttgaattgtt atctgttccc
    25741 aaagaatatc aaatttatct tttatagata tttcttgtat caattctcta gtttctttgg
    25801 gtagatactt tgagaagaaa ccaaactttt cagcattctt attctctttt ggagcaccat
    25861 gaccaactga atttttatta gaaaagggtg cacctctttt atttataggt gcacccttct
    25921 ttttttcact agcccagttg tatcttttta tccatgactt taaagtgttt aaactaatgt
    25981 catactttga tgatatttcc ttttgtttca ttccttttaa gtaatcttgt tttacctttt
    26041 ctttgacatc ttgcacatca ccacctcttt atttgtttgt tttgggaata aaaaaacaat
    26101 cctatttaat aggattgttt caaatctagt ctcctctatc cttccaggat aaatgaaaca
    26161 tacttattat taatgtagtt agagataaaa ttatatgaac tgtaaaaaat attaaaacat
    26221 agaaaattat attagaataa tatcgagtaa gcattattgt attgataaaa catattacaa
    26281 caaccaaaat ctccattatt acagtataat atgcttttaa ctgtatgaat tgaaagtatg
    26341 atatattaaa tccatttacc tttctttctg tttttatatc ttttgcttct gatattgatt
    26401 tgttatatgt agtaaacaat aatgtaataa tgcaaactcc aaaagctact aatagtcctg
    26461 acaaagttag tacatctttg tttaaagaaa ttacaacttc tttattaatt gacgaattaa
    26521 aaaacatgta tgaaataata catattaata ctggtgttaa aactaactct actacttctt
    26581 tagaatttat agccttgtaa taattgagta agatatttaa taccttttta tacattttaa
    26641 cacctccttc gcaatctatg cttcactcat agcaacttct tgatacatta aattaaaaaa
    26701 aactgaatta ttctcaatta aatcatctat caaattattc atcttatcaa aaatatcttc
    26761 actatctata tgattatcta tatcaagaga aacctctatt tctctacaca actttatacc
    26821 ctcagtatcc aactttaatt ttctcttgtt tttctctccc tcaattatta tcctatttat
    26881 ttttttattt ctaaattcct ctcctttaat atatttttcg caacatcttt taacttcact
    26941 aggtaaatat ctagttctaa atgtgggctt atatatcaaa tcgttatgtc gtcttgaatt
    27001 atttaaatta gaaaacaaat tatcttcatc agttccaaac ttctctctat caacatttaa
    27061 agttattttt gaaacaccat ctaaatctaa tatttgttct ataaaatctt tacttgctat
    27121 ttgtgtaata ttaattgatg tttttgacat attattatca aatttattat tttcaatcat
    27181 atcctcataa tatagatgta agtttttact tagttcaatc atgggaaccc ccacaaaaac
    27241 tctttcaaat aatactactg ctatattatt ttccttcata aatttaataa ataggtgctg
    27301 cttattgcta tctccttgat ccattttttt attaccctct acttctttag tatgagcatt
    27361 tataatattt gtattctgat tgtatttagt atattcaagt ttcaccttga ttattaaatc
    27421 atcctcgcta cattttactt ctgtaatcca aaacttttta tctttagtaa tattaattcc
    27481 atttttgttt tctttacaat attctttatc cttaaagaag gtctttatta cctcttttaa
    27541 aatttcttca tctgaataga cactcccatt aattttattt ataatatcta ttctataaac
    27601 atcaatatat ctttttttaa aattactcat tataccatta ttcctttcac aaatatttat
    27661 aatgatataa ttctatataa tcagtcaata tccttcaata atcattcgac aaccacaaaa
    27721 taagtctaaa taatactttt accttattcc tgtctataat gtacaaatat atttcatttt
    27781 taattcatta cataaaaaaa agaccctcca ttaatacagt catttaaatc atttctatta
    27841 attctttaat cttttatata cctctttata attcatactt ttatctatca acctaggtga
    27901 tttcatagat ataactcttt caagtgcttg tattaataaa caaactcttg tatttcatct
    27961 gtaaaatctt ttctgacctt tttaccttct gttcttatat ccagccattc ttgatctttt
    28021 tctgtggcaa tatagtaacc atgtattcta atttgtttaa gtgcttttgt aacccacttc
    28081 gtaaatagtt ttgcttctgc tttattactt ctaaatgaca tattgtacac agcttcttct
    28141 gtaacaaaag tagtaccaaa gttaggcaat ttatctttaa agtttctagt gtaggaatct
    28201 ccgacagtag actcattaat ttctttttat attctctatc tatatttctt aatgtatcac
    28261 gaatatttac tatgcctagt tcctctccta cgtcatttgc attaaatcaa acttcttctc
    28321 cattttttga ccatatcatc cttacatttt tctcttgtaa aattttcaac atactactac
    28381 ctcctaaatt agttattaat aaagaatatt caatttgaac acccttattt tcgacccttc
    28441 aatatgaggg attaaaattg tataaaaaag acctagaaat cactctaagt catgaattac
    28501 tcatttatca ttctccttat ttacaaaata aaaaagacta agttggggta acttagcctt
    28561 tttcaagggg gaatatatta tacacttgtt tcatactacc attataacag ggttaaaata
    28621 tcattaaaat atcatctttt tatcatatat ttatcaaaca gttaatttca agccatctat
    28681 accgaataaa tacactccca gttcctttat catttcatta atccaacgtc ttgatgtaac
    28741 tactccacaa tttaatatct ctgctatttc ttcatatgtt ttttcatcac aatagtaaag
    28801 ctctaaagct ctatactttt cataagaatg taaacgctct tgtcttaatt tcaaagtttt
    28861 cattgctgaa tctatatgtg ctatcataat aatagttctt gctttacttt tctttatact
    28921 taaaatatac aactcttcat tatcaagctt acctaaatca tcctctaagt aatctacatc
    28981 tttagcttca cttaccgctg agtctatatg tctcttaaaa tcattataat gcttcatcaa
    29041 taatctagta ttatataaaa cgcctttttt cctatcttct ctttcttctt ttcttaactc
    29101 tcttacaatt tcttttatac tttctttatc cacttaaatc actccttctt gctctcttcg
    29161 aacaacttct actgcaatac ttaactttag atttatccaa cttataaaat cttctaccac
    29221 accaagcaca tcttactatt ttacctgggc ttataagttc catcttttct ttttactttc
    29281 ataccttcac tccctatatt cttttaattt tgctttgact gcttcaagta atgcactttg
    29341 tcctttatct ttattctcta gagcctccat tacttgttca tctattgtac ctttacagat
    29401 tagatgatgg ataataacag tttccctttg cccctgtcta taaagccttg cattggcttg
    29461 ttgatacagt tctaagctcc aagttaatcc aaaccaaaca attatacttc cacctgattg
    29521 taaattaagt ccatgtcctg tacttgctgg atgacaaagt aataattgta ttcttccatt
    29581 attccaatca tatatatctt ttgagttttc tattgttctt ggtttcaaac tcttaaactc
    29641 tttcattaaa cgattataat catgtttata gttataaaaa actataatag gcttaccatt
    29701 tgaaacatct ataatttctt ttaaagcttc taatttttct ctatggagtt cctttacatt
    29761 tttatcatta tcataaattg ctccattagc taattgtaaa agtttatttg ctgctacagc
    29821 tgctgatgaa gctgttatta tatctttttc taattctaat atcttctctt tttctaattc
    29881 tttgtaatat ttaagtattt tactatcaag gtgtatttca attttattat ctattttttt
    29941 aggcatattc aaataatctt ctgcttttaa gcttatacaa atatctttta ttttattatg
    30001 tattgcattt tctgctccat ctttaggctg ccaattatat atagctcctg tttgataatt
    30061 tttttgtcct ggctcaaagt atcgttcttt atatcctgta atagttttac ctagtctttc
    30121 acctctatca agtaagtaca tctgtgccca taagtctatt aaactatttg gtgttggtgt
    30181 accagtaagc ccaactactc ttttagttaa aggcaatact ttctttaaac ttttaaatcg
    30241 ttgtgcttta tttgatttaa aagaactaag ttcatctatc acaaccatat caaatggcca
    30301 ttttctttta taaaaatcca ctatccaggg taccatttct ctatttatta tataaatatc
    30361 tgagtctgta cttaaagctc tcactctatc taatttacta ccaagaactt tagatacttt
    30421 tagatgcttt agatgctccc atttttttac ttcactactc caggtatctc ttgcaactct
    30481 taaaggtgct ataactaaaa cttttgatat atcaaagtaa tcatacatta attcacttat
    30541 agctgttaga gcacagactg ttttacctaa tcccatatct aatagtaaac ctatattatt
    30601 attatctata gtttttctaa ttgtgtattc ttggtatgga tgtggtttaa attccatccc
    30661 ttaacctcct tgataaaatc atctatctct tttaatgtgc ttacacactt aactttaaat
    30721 cctaattctc ttaattctcg cattttatat tgttgaagct ttcttagttt tttacctggt
    30781 gctttaagtt ctacaaatat aacatgtcct tctggtaata agacaatcct atctggcaca
    30841 cctgcctcac ctggtgaaat aaacttcata gcctttccac ctaacaactc aatctctttt
    30901 ttaagtcttt tttctatttt tgattctaac aaaatatcac ttccttaaaa tttaagtgtc
    30961 aacaatgtca acagattttc tatatatatg tatatataca tattaggcat acatatgtat
    31021 atacgtatat gcctaatatt acttatacta ctttatactt aatttttgtt gacattgttg
    31081 acattaatac ctcaaacatt gtaattactg catcttaggt gtcaacataa gcgtcaacaa
    31141 agccattttt tttgttgaca ttgttgacac ttttaaaatt ttcaaattta ctctattttt
    31201 aattctgttg acactaactt ttacgtacat aagctctctg cctaccatat attttcccaa
    31261 accttaaatt tccattatat gatatccaat catctaatcc ttttaatata tcattaattt
    31321 ctcttgataa gataggcgtg agttgtttag gttctccatt aaatagctca acccatatct
    31381 ccataacaca cgttttttct cttaatattg tcccttcttt taaatcacca aaatctgaac
    31441 catgaatata ttctctcttt tctgaaatac ttaaatcata ccaattctta gtaataggtt
    31501 tatttagata ctcttcgata attccagatt tagcattttc ttctgagtga gttctttgtt
    31561 gtctttcagc ttcttttttc tcctcatctg atagatatag ctgttcattg gctttatata
    31621 attctactgc ttctgcccaa atttgatttc tttcattatc aagttggcca ttaaatatac
    31681 tcttgttagt cttctttact cctgcatcta ttggccaaaa ccttctattc ccagtcttat
    31741 ctcttaaaaa ttctttatca ttagttgttc ctataactac acattgacgt aaaaatcttg
    31801 aggttctctt cccatatgcg accctatata tatcctctga tttgcttaga aaatgcttaa
    31861 ctgcctcaat atctgctttt ttagtagcca tcatttcacc catttccagc aaccatactc
    31921 cttgtaattg ttcatacgct tctttacctt gtacagtagt taaactatca gaataccagt
    31981 ctccaccaag ttttttaata aaagtacttt ttcccattcc ttgaggacca gataaaacca
    32041 tcatattatc aaactttatt cctggattaa atactcttgc tactgctgct actaaaactt
    32101 ttcttattat agtccttgta tagtggttat cttctgcacc taaatagtca attaaaagtg
    32161 tatccactct cttaatgcca tcccacttta aagaatttaa ataatctttt ataggatgaa
    32221 aagtattatt ttcaaaagca attattagag catcatttac ttttgatggt gatgagatat
    32281 tgtaaattgt ttctatatga tgtctaagcc cagaatcatc actatcattc caatcattta
    32341 atttatcatc ttttctccaa ggtaacttac ctaaaacgac agctctattt gaaaattcat
    32401 tataagctat ttttcctttc aaatatgggt cattttctat aaacattaag atattgtttg
    32461 ttgttttctt ataacttccc ttattgtcat aatctaacct agttaaccat tcatcatctt
    32521 caaaatctat atcaccaaaa tcatccttag ctttatcaag gttttctctt cctatagtct
    32581 ttcgtacttt agtgtcactg cttgcaaatt cgctcattcg agtaaatgaa ggtaatctat
    32641 ttacaggcgt ttcgggttta gcatcttcat caagttcacc aaatttatgt atcctaacta
    32701 aatcaaaagc attgcataaa attccgcttg ctgggtctgt accatgatga ctatatgaaa
    32761 acttgtcatc ataaataact actccaccac ttgtactacc ttctgaatat gtatacctgg
    32821 tttcatcaat accaggaata tatacttcat ttaagaaagt ttctatagtt tcttttatac
    32881 tataggacct gcaaaatgca cctataattc cagccttttc aatagggtct tgttgttttt
    32941 ttaattgtgt attaaacttt tgcctctccc tcgaactttc tggccaatag cttacatctg
    33001 tccagtctaa atataaatct agtatttcat ttgggtttaa aaactctcca tcttgaaatt
    33061 taaatatata gtctccatca attgaagtac ttggaaaata cataagtcta tgaggttgat
    33121 atgtagtatc atcaaacata tctattccta tagtatctgc tatcatcctg gaaattgctt
    33181 gatattcttc tggtagtact ggtctagtta aaggtattac aagtctatat ctgggattat
    33241 tctcagtatg tgagtgcgta gaatacataa gacaagcata atcatttaat aatgttatat
    33301 cctcccatat atctttattc gcataatcta tatctaaagt tataatactt ctatttgcaa
    33361 tattctctgc ttttcgtctt ccatttttta agcttccacc tacaaatcca ccaacatctt
    33421 taactctatc tttttcagtc tttgacatct tcctatattc tgtatatgtt tcttgagttc
    33481 ttaatgtttt acttaatcta tttacaagtt cggaccataa aatactttta tttttccaat
    33541 gtgtttctaa tttatttttt cctatggcca gcatgagttg gccatcatgt cttacattta
    33601 tgtgttcaat ttcactggcc tttatatcca taaatcaatc acctagtctt tcttataata
    33661 atcacattca tatccatctg ctttaagagg aagaccttta gcccaagata tttcttttcc
    33721 cataatactg ttaacttctt ctaaagaacc tcctttttta tctacatcaa ttacaagctc
    33781 atcatgtaca tgcattacaa tgctataacc tgcatctgtc acattaaaca tagcctctct
    33841 taagcaatct ctagctgtag cttgaacaat attctcaact aacttaggtc cataagtatc
    33901 tattcttttc cattgtttac ttgtctgttc cataccttca tatgttatct tatccccact
    33961 aaatgtagta tgtggctcta tcttaggcct tagatacgat aatcttctac tacttggtag
    34021 ttctataaat aaaacgcctg gattataaat aaatttaatc ccatgttgaa tttctactat
    34081 agtcctatct tttatagctt ttttagctgc cttatctaca tcccaccaaa acttagttat
    34141 atttggatta gcatttctcc atgcagtaac aataggttga agctcttctt ctttaagccc
    34201 cattttaata gcccccatag aaattaaggc ccctacactt ccgccatatc cattcgataa
    34261 ttctgctagc tttccttttt gtctaagttc agaacctttt ttaatatttt cgattggaat
    34321 tttaaacatc tgactggcac tagcttcata tatttttcca tgagaattaa acacatccag
    34381 tctccacttc tcacctgcaa gccaagctat aactctagct tctattgcac taaaatctga
    34441 cactataaat ctatgacctt cacttggtat aaaagctgtc cttatcaatt gacttaagac
    34501 atctggtaca ctatcatata aaagctctat taaatcaaaa tctccttctt ttaataggtt
    34561 tctagctagg tctaaatcct ctatatgatt ttgtggtaaa ttctgtactt gtactaatct
    34621 tcctgcccat ctaccagtcc tattagcccc ataaaactgt agtagacctc ttactctatt
    34681 gtcattacct ttagctaatt tcatagcctc atatttcttt atagaagttt tggacattaa
    34741 ttttctaagt tctaaaattc taactacatt ttcatcatca acttgtttta atatttctgg
    34801 aatactttct tttgttaggc ttgtaatctc aaagccaact ttatcactta accatttttt
    34861 taattgagct ggactatttg gattatttag accagttatt ttaattgctt cttttgtaag
    34921 tttttcagta tatcttttat cacattctat tgcattctct attaactctg tatccacttt
    34981 aataccagta tcattaattc tctggtctaa ataccataat ttaatttctc tttcggtagt
    35041 cttatactta ctaagtttgt ttcttatttc tctttcaact acaacatctt gtttacaata
    35101 ttctttaaat ttattccatt tttccatatc atgtattggt aaatttctag ttcttccctt
    35161 gttaactttt gtagccttac aaggtttaca gaaatattgt attaatgctt taccttcttt
    35221 cattttctgt ttatcttcat taaactttaa agccttagac acactatcta aacttcctgg
    35281 aagtcctagt gttaacgcct ttatcattgt acatgaccac tcatttggtt ttaaattaat
    35341 atttaaaaat ttacttattg atgttctttc aaaattagca ttaaatgctg attttataac
    35401 tttattatca tttaaagctt ctattacttc ttttggtaac tcttcatcat ttactaaatc
    35461 aataactttt acctcttcat tatcaaaagc ataggcaaat aacagtatct caaaattagc
    35521 agagtctaca tatctgtaga ctccaacttt ttttatatct aaatcactat atgtttctat
    35581 atcaattgat aaggtcctca ttaacttaag aagtcctctt cttcatcttc atattcaaag
    35641 tcatctgaga aatctgcttc tgcactagct ctagctcctc caagtacttc tccatctgct
    35701 aacttttgaa cattttgtaa gccacaacct attcctttat ttcctgcact attatatggg
    35761 aaaaagttta tactaactct tccataacaa ccactatata cctctgtatt atctaatatt
    35821 tcatttaagt ctttatcaac tattcctggc ttttgagtac tatttgcatt taaaaaatac
    35881 attcctacat attcctctgc ttcatcagct ctttctgcat ctccatcacg aagaggtgtt
    35941 tttaaattac ctggtaattt tccaccccat ttagaagttt taccttgttc tttagctgca
    36001 tcaatagcct tctttattct tcctaaagtc accttatctg attttggtat taaaatacaa
    36061 actgaatact tgggctctgc accttctacc attgctctgc ttttaaagat attgcaataa
    36121 cttaatctta cctttcctgt tactaccttt gttgattgta ctgaattact cataattttc
    36181 ttcctcctaa tatttttata tttggtatat tgattttata tttgaagtta tttctccaaa
    36241 tttatctaaa tctctaactt gcttactaaa caatttaatt cttccacaaa taactgttga
    36301 agaaacattt aataactctc ctatatcaga tatcttttta tcttgtaaaa tccaatctac
    36361 tattttatca atatctttta aatatgactt tcttgcaatt tttcttatat aatcaacatc
    36421 atagttaaat tgaatttcat aaaatccatg tatatctttt aatggtcttt tactatcttc
    36481 aaatacactt acatctaaac cttctataga gtttctataa tgtttttcgc cataacgcaa
    36541 agctttaaac atttctattc ttatgtaaga aacagccact gtagaaaact tgcctctgtt
    36601 actatcataa tttaatgctg ctttatataa cccaatacat ccttcttgat aaaactcttc
    36661 atataagtat ggatgttgct ctacataaga cttaaaaaat ctatttatac taaaatgtac
    36721 aagattcaaa ttattttcaa ctagtttagt tatttcttga ttactcataa aattatatcc
    36781 ccttttaaat ctttttatag ctactaggaa ttaaatattt ttaattatcc tttgctctta
    36841 ctgatacaaa gcatactgga ctaactttta tatgttctgg tatatacact ctatcaagat
    36901 gattgtcatt tacttcgagg aaagttgaca tcactttatc ctttattaaa ctttctcttt
    36961 taaaatccat cactataact tttgcatttt cttcagttga gttatattct gtaatattta
    37021 gtatctttaa catatctagt aaattatctt tttcataatc taaatctttt tcgatactct
    37081 tttttctatc ctttaaatct aaaattatac ttatgctttc atctaattta tcttttatac
    37141 ctgcatctaa tggcatgtaa tatcccccat ttatatttca aaatcttttt tcgctgaatc
    37201 tatactatta atttctgacc ttttatcact ttctactact aaagtagctt ttccaactgg
    37261 ttttataatt aaatcactta gtagtttagc aaaactcttt ttacctatag ctttctccat
    37321 atcactaatt ccttttaagg tccttggctt ataaattttt tcttcatcat aatctgaatt
    37381 taataaaacc ttagcaactt cttgctcatc tacatacttt ctattacttc tgccttctac
    37441 aagcttatat cctggatatt taacaccatg cttttcagct tgttctagtg cataactttg
    37501 aacatctttt aaccaatctt gtatattctt agcaaaacct aaaatatctg ctatctcata
    37561 tttattaaga gcaaatgtgt cagcaaaatc atattttcta gctagtttaa ggttatcctc
    37621 agctcttttt ctgcaatcat ttttagctct acaaaatcca caatgactgc cacttacaaa
    37681 ctctccttca ccattaaaag ccatttgagc cttcttctta acattatctg cccatgtaag
    37741 taatttagta acttctattt cttcacttga tatgttatca agtctaggtt gaattattgt
    37801 tgttttaatt aaatcaatat cgtatagcat ttcaaactga ttatatgccc ctaaaccata
    37861 tagtctaagt tgaggattct ctatagctga aacttctaaa ccttttccat actttaggtc
    37921 tataacttga agtataccat cagatattac aacaacgtct cctgttccaa atccttctgg
    37981 aacccactca ctaaaatcga gtctttcttc taacatcact attacatcat cacatatagc
    38041 tttactatca tttactaatt caactacatt ctcaacataa gattgtatat agtcctccat
    38101 ttcagagtta taatactcac ttttctgtat ctttttaatt cttgcattat atgccttttt
    38161 acttattttt tcatactcta gcattaattt aacttctgct aattcatgtg caactgttcc
    38221 ttcttctgca tatatactag ttgatggtgg atagttttct tctaatttta tactaggagt
    38281 gcagtgaagc catctatggg ctccactcgc actaagtctt gcatgttgta atggcattta
    38341 tgtaccccct ataaactttc taatttgttc ataaaagctg aataatcttc ttcttttact
    38401 tcacttaact tactagctcc aaattcacca aataactcct taagcttatc cttcttacct
    38461 gccttactta cttgtgctgc cttagttctt acctcttctt ttgtgtattt aacttcacta
    38521 gttgtatttt cattattctt ttctatttct tcctttgctt ctgctatttc ttctttaacg
    38581 tcttctttta cttctacctt tttaatcttt tcagcttttt taacttcaac tttcttctct
    38641 tctttcatat ctgttatatt tataggttct atctgcattg cttttcctag attaagacct
    38701 cctaaagcat tcgccactac taatagtgca tttgtaaatt ctggtgcttc tactttaact
    38761 tttacattta cattcacttc aaccatttta aatctctcct ttttgtgtta tactttactt
    38821 gtgttatatt ttatttttta tttttttgtg tgttggttat tttaccaaca cttttttatt
    38881 taatattcca accgatattt tcttaccagt tttaatatct ttaaatacta tatctgctat
    38941 aacttttcca tcttttttaa gagttactac attcttatta ttagtatcaa gactcagcaa
    39001 tttcatcccc cctctctact gcttttaaaa gttcatccaa atttttacct tgatttcttt
    39061 caataaaatc atcaacttca tatcttgaaa tttttctacc atcacctcta actagtgatt
    39121 ttatcaaacc tgtgcttact aacctacgca taaaagctgt atctaacttt aaaattcccc
    39181 ttgcttcttc tactgttatt agataatttg gataacctct ttttattaaa acaactatat
    39241 ctttaggctc cagtaccttt accttttgtt caatagtttg actcttaact ctatctgtct
    39301 cttgtttact tatctctatt tctattaagc tttttaagct atcactaaat tgttttgtta
    39361 tgctttcaga aatatccatg tgaaaatgct ctcccctctt ttcaattatt tatcttccaa
    39421 ccaattcgtc taatgtaata tctaaataat cagctaattt tattagagta tctatagttg
    39481 gattagaact tttatttctc agtaatttat ataaaccact tacatctata tccaattctt
    39541 ttgccaattt ataaggtttt atatctcttt ttcttagtat ttgctttata ttgtcgccta
    39601 ttaacattgt attcacctca gttctacatg gtataatata aacatggaat aaattccaaa
    39661 attttcaaaa ggatggtgta ttatgcagtt ttcaaacaat atatctaaag aattacaaga
    39721 aagtataaga aagacagtct ctgatacttc taacacagag tatttttact atgctgaatt
    39781 tcaatataaa attattctta aatctataga ggaatttgaa aaagaacttg atgatgaaca
    39841 tgaaattgca ctaaaattga ctaactttgg aaaagatgta ttaatgattg ttgaagaagt
    39901 aggttatcat aacccttgtc taattcacta ctacggcata gtaaatggag tttattctga
    39961 aattcttcaa cacacatcac agataaactt tatgataact tcagtaaaga aaactgaccc
    40021 ttcaaaacct gctagaagaa taggttttat attgtaatta tcattcttat tttctgattc
    40081 tttctttagt aatttcacta atttttcttt tttcactttt actcatctca tgtaaataca
    40141 aaactataca ttggtacata taatctataa aatcactatt tgattgagct gttatctgac
    40201 taaataactg tgcttgcaat tgattttcaa gcatggttat tcttttctct atttcttcta
    40261 ttttcttatt ttccaattca atctctcctt tccaaaatat tctgtattta gttttcaaag
    40321 tactaaccta atattgaagt ttgataacta tattcttttt ctatatctgg taatatgtta
    40381 tttgttttca ataaatcata taaaaacaat ctaccttttt gagtccactt agttgtcatt
    40441 tttacatcag gcattccatt acttctagtt atgtctattg tttctgaatg agtgtatccc
    40501 ttcccttgat gttgtttata taaaagccat tgtccacttt gtttatattg aatccctctt
    40561 tcatgaagta ttttgttcat ttcttttcca ctcattccat aatcctttgc tatttgagtt
    40621 atagtgacta gacctttgtt tttaagtatc atatctgtat aatctgcctt tggttttagt
    40681 tcttttatta cttggtcttt catttttcct tctagttgta atttctcatt tacttctact
    40741 tgctctataa gatgttgtaa tgcttcttta tatgtagttg gtagtttagg ttgttcattt
    40801 tttaatactt gctccatttc ttcaaatttt tttgtatata ttcctgtaaa tgccgtacct
    40861 tttatgcctg tcattttatt tgctatgaag tcacaaccta tctttgttat tgcatagcat
    40921 ggcttctctc ttttataatc atctaaataa gtattttcaa taaaataatc agatgggtac
    40981 aaatctgtac tcatgttttt attggcttct tccatttggt ttatataatt tcttatatcc
    41041 cttagtaaaa tcttgtgttc tttttcaacc attagagcta tatctctact gtcagttgtt
    41101 agtttattat taacttttac tatttttaaa tctttcatat ttaactcctc tcttttaaac
    41161 aacatcttgt tgtatttgta tttaaaaaaa ttttttctat tgatactcca aaacatttag
    41221 ataattttat tgctattgct aaacttggta ctcttgttcc actttcaatc attccataat
    41281 aactagtagt tatacctact tttaatgcaa catctttttg ctttaaattc ctttctgtcc
    41341 taagttcctt tagattgttc aagctatatc ctccttacca acattttgtt gtttatactt
    41401 atataataca caactatatg ttgtttgtaa atagtttttt tattttttta tttcatttta
    41461 caactatttg ttgtattatt taataaaggc aactttatta agaggagtga ttatatgagc
    41521 attttatcag atagattgaa atttcttagg aaagaaaaag gtgtaatgca aaaagaaatt
    41581 gccaactatc ttaacataac tactagtgca tatggatttt atgaacaagg taaaagaaca
    41641 cctaccccag aaatgttatc tagtttagct gaatattttg gcactactgt tgattattta
    41701 ataggtaggt atgataataa agcaagcaat atttctagca aaacttcttg taataataca
    41761 ttatttcaaa aaagactaaa agaacttaga gctgaaaaaa atatgactca agaagatgtt
    41821 gcaaataaat taaacttaac caaaagcgct tatggttatt atgaacaagg aaagacagtt
    41881 cctgatgctt atatgttatc tagccttgct gaaatattta atgtgactac tgattattta
    41941 ttaggtaggt ctattgtaaa aaatgatata gacactgtag ctgcacatag agttaatcct
    42001 cacaaagatt taccagagga agctcaagaa caactcaatg attatattga atttttaata
    42061 aataagtata aaaaataaat taaaagtagg tgataaatta tatgaaatct ataatatcag
    42121 atgtcataaa ccgatttaat gctacaccat ttttatttgt tggttctggt ttaactagaa
    42181 gatactataa tcttccaaat tgggaggatt tactaaaagt ctttgcagaa aaaattagta
    42241 atgatgactt tatatataca agttataaaa ataaagcaaa atcagagaac ccaaaaatgg
    42301 gtataaatcc tagaattgca gaacttattg aaaatgattt taataaaaaa tggttttcag
    42361 attcttctat acgaagctta gactctaagt atttagatat tgttaaatca ggagtttctc
    42421 catttaaggc agaaattgct atgtatatta aaaataattc taaaatagta gataaatatc
    42481 aagatgaagt tgaaaaactt agtaatatct ctaaaaaaag tttatcagga tttattacaa
    42541 caaattatga ttgttttttg gaaactatcg ttgataatta tacaacatat ataggtcaag
    42601 aaaatttagt tttttcatct atacaaggta ttgcagaaat ttataaaatt catggatgtg
    42661 tttcttctcc aaatagtatt gtaataaatg aagctgatta tatagacttt gacagcaaaa
    42721 gtgcctactt ggcagctaag ctaatgacta tatttgttga gtttcctatc atatttattg
    42781 gttactctgt tacagatgta aatataaaaa aaatacttaa tgctatagtt aattgtctat
    42841 ctaatgaaaa tgctaaaaaa ctcgaggaac gtttcatatt cattgagtat gaaaaaaact
    42901 ttaatgatat agaaatatca agtcatacaa ttgcttttga taataaaatg attactatga
    42961 ctaaaattaa actggaggat tttaatttac tctatgaggc attatctgaa aaaaaatcaa
    43021 aattaccagt tagaatatta agaatgttca aacaagaatt ttacgatttc acaattacta
    43081 acaaacctac tgctaaaatt cgggttggta gtgttgatga tacaagaata aaagatgaag
    43141 atttagtttt agcagttggc aaggcaagtg attttggtct taaaggatta aaaggattaa
    43201 gttttgatga atggtatcgt gatatagtca tgaatgactt agaattttca tcagatgaat
    43261 tacttgaata tgcttatcca tctttaatta agcaatataa taaattacca ttaaataaac
    43321 atctttttaa tagcacactt gaatttccag attataggac aattgcacta gaaagtgact
    43381 ttgaaaatat tattagcaat agtattaaaa aaaatagaaa taatacttac ataaaaaata
    43441 gaagtgttta tggtatttgg aatgatgaac atcaatcatt tgaaaaagct acaagactta
    43501 ttgctttttt agaagaaaaa gaaattgaca ctgaacagtt agaaaatcta cttaagaaaa
    43561 tatttgagga aaatcctaat attttagagt ctgcaaaaac aagcgaaaaa acaaatttaa
    43621 gaaggcttat tagaatatat gactacatga aatactctaa tatgcaaaag agtcttggtt
    43681 atagtgagta aaactctacc caccgctcca aaactccttt aatcgcttta agcacaaata
    43741 caaatttagt gctaactata ctaacacaca tttgtaagtg cttacatata ttatatgtaa
    43801 ttatattata atcatatact ataatccata ttttgtctac agctaaaatt atccaatctt
    43861 ttttggataa aaaatgaata tttagagcag ttcacacctg ctctttatat ataacaaaaa
    43921 tcaaaaatac attcttttta tagggggatt tcaatgaaca aactagacgc acttttagac
    43981 ttagcaaata atgaagagat agaaatttac tacactgaca aaatagcaga tgacataaaa
    44041 ggattgtata taaacagaca aggactaaag attatatcat tacttaattc attaaaacaa
    44101 aacaatgcta aactaataga aatcttagca gaagaattag gacatcattt taccagtgtt
    44161 gggaactatg tatcttcaaa aaacagttac aaaaataaaa tcttgataga caaaactgaa
    44221 aacaaagcat taaaatgggc atgtgaattt cttataacag aagaagaaat aatacatgtt
    44281 attaattcac acgctacaag tgtatacgaa atagctgaag aattacaagt tagcatcaac
    44341 ttcttactaa aaagattaga atttctatca aaaaagaaaa gcatgttgga cttaggaaat
    44401 aatagatttt tagtattaac taatttgcca aatttctaca tatatgagga tattttttaa
    44461 actcatttat tctactttta tagatttttt acttaataaa tatatatttc aatattatta
    44521 taataaacta cacataaaag ctaaaaaatt gtaagaatat taagaaaata attaagtaaa
    44581 aaccagataa acaaaattaa gataatattg tacataacaa aagtatataa agagcagtta
    44641 atctgctctt ttatataaac accaaacaaa catacattct aaaagggagg gatactatta
    44701 tgaaaggtgg agtaagaaaa agaagtaaca aatggtatta ctactttgac ctaggcatag
    44761 tagaaggaaa aagaaaaaaa gtagaaagag ttggaggcaa tactaaaaaa gaagccgaaa
    44821 aagccttaag agaagcacta aatgaatatg aaaattctgg catagtattt gaagaaagca
    44881 atatcagttt atcagactac ttagactttt ggtacaaaga atatgtctta cttaactgta
    44941 aatacaacac tcaagaaagc taccgaataa acatagaaaa acatataaag ccaaagctag
    45001 gagcttacaa agtaaaagct ttaactcctg caatactaca aaacttcata aataaaaagt
    45061 acaaagagga ttactctcaa aatacattac aagtattaaa agccatatta cataggtcat
    45121 taaaatcagc agtccatcct tacaaacaca tacgagaaaa ccctatgcaa tatgtaagca
    45181 taccaaaaac taaatctaaa acagaaacta ataaagttaa aactattaca gtagaagaat
    45241 ttaatcaaat actaaatata tttcctcaag attcatttca acgtatagtt ttactaattg
    45301 gatttcatac tggtatgcga agaggtgaaa ttattgcact aaaatgggat aatatagacc
    45361 ttaataataa aactatcaca gtaagacata ctctgattaa aaagccaaat ggaatgtttg
    45421 aattagggca accaaaaaca gaaagctctt gcagaactat atttacaggt gacactttaa
    45481 taaaggcatt aaaagaacat aaattatatc aaaagaaaat gaaattaaaa tatggagaat
    45541 tttactttga tagtgactgg gtatgtacca aagaaaatgg tcaacaagtg aatactcaca
    45601 ctttagacac tatagtaaga caaattcgag tagctttaaa caatgacttc cattttcatt
    45661 ctttaagaca tgcacatgct actctattat tagaaaatgg tgctaacatt aaagacatac
    45721 aaaaccgttt gggtcatagc caactatcaa ctacaatgga tacctattca catgtaactg
    45781 ataaaatgaa aaatgaaact gtagatatat ttgaaaaaat tacaaattag agtttgccac
    45841 ccaaaaatat aatacggtgg caaatgggtg gcaaaatcta atttatctat tttaaaagct
    45901 aaaattatca aatttatata gtcagctata cgcatgtaat ttcaaggctt tagagtatat
    45961 aacaaccata actaatataa ggtattaata ataaatctaa tgaataaaac tta
//
LOCUS       DAMJYX010000021        44083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJYX010000021 DAMJYX010000000
VERSION     DAMJYX010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.021"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(244..1599)
                     /locus_tag="PC479_001292"
     CDS             complement(244..1599)
                     /locus_tag="PC479_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2803845.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(1625..1894)
                     /locus_tag="PC479_001293"
     CDS             complement(1625..1894)
                     /locus_tag="PC479_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2803846.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(2184..4139)
                     /locus_tag="PC479_001294"
     CDS             complement(2184..4139)
                     /locus_tag="PC479_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803847.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(4322..5797)
                     /gene="spoIVA"
                     /locus_tag="PC479_001295"
     CDS             complement(4322..5797)
                     /gene="spoIVA"
                     /locus_tag="PC479_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2803848.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(5969..7264)
                     /locus_tag="PC479_001296"
     CDS             complement(5969..7264)
                     /locus_tag="PC479_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2803849.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(7448..8857)
                     /locus_tag="PC479_001297"
     CDS             complement(7448..8857)
                     /locus_tag="PC479_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2803850.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGC"
     gene            complement(8909..10048)
                     /locus_tag="PC479_001298"
     CDS             complement(8909..10048)
                     /locus_tag="PC479_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2803851.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            10367..11017
                     /locus_tag="PC479_001299"
     CDS             10367..11017
                     /locus_tag="PC479_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2803852.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(11174..12193)
                     /locus_tag="PC479_001300"
     CDS             complement(11174..12193)
                     /locus_tag="PC479_001300"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2803853.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(12224..12838)
                     /gene="plsY"
                     /locus_tag="PC479_001301"
     CDS             complement(12224..12838)
                     /gene="plsY"
                     /locus_tag="PC479_001301"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2803854.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(12873..14192)
                     /gene="der"
                     /locus_tag="PC479_001302"
     CDS             complement(12873..14192)
                     /gene="der"
                     /locus_tag="PC479_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2803855.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(14226..15554)
                     /locus_tag="PC479_001303"
     CDS             complement(14226..15554)
                     /locus_tag="PC479_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2803856.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(15792..15992)
                     /locus_tag="PC479_001304"
     CDS             complement(15792..15992)
                     /locus_tag="PC479_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803857.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(15994..16926)
                     /locus_tag="PC479_001305"
     CDS             complement(15994..16926)
                     /locus_tag="PC479_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2803858.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(16971..17903)
                     /locus_tag="PC479_001306"
     CDS             complement(16971..17903)
                     /locus_tag="PC479_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2803859.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(17958..19247)
                     /locus_tag="PC479_001307"
     CDS             complement(17958..19247)
                     /locus_tag="PC479_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803860.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(19276..19968)
                     /locus_tag="PC479_001308"
     CDS             complement(19276..19968)
                     /locus_tag="PC479_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803861.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(19983..20744)
                     /gene="pgeF"
                     /locus_tag="PC479_001309"
     CDS             complement(19983..20744)
                     /gene="pgeF"
                     /locus_tag="PC479_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2803862.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(20741..21202)
                     /gene="nrdR"
                     /locus_tag="PC479_001310"
     CDS             complement(20741..21202)
                     /gene="nrdR"
                     /locus_tag="PC479_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2803863.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(21224..21487)
                     /locus_tag="PC479_001311"
     CDS             complement(21224..21487)
                     /locus_tag="PC479_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2803864.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(21591..22364)
                     /gene="sigG"
                     /locus_tag="PC479_001312"
     CDS             complement(21591..22364)
                     /gene="sigG"
                     /locus_tag="PC479_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2803865.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(22456..23178)
                     /gene="sigE"
                     /locus_tag="PC479_001313"
     CDS             complement(22456..23178)
                     /gene="sigE"
                     /locus_tag="PC479_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2803866.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(23203..24075)
                     /locus_tag="PC479_001314"
     CDS             complement(23203..24075)
                     /locus_tag="PC479_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2803867.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(24365..25639)
                     /locus_tag="PC479_001315"
     CDS             complement(24365..25639)
                     /locus_tag="PC479_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2803868.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(25923..27080)
                     /gene="ftsZ"
                     /locus_tag="PC479_001316"
     CDS             complement(25923..27080)
                     /gene="ftsZ"
                     /locus_tag="PC479_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2803869.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(27270..27623)
                     /locus_tag="PC479_001317"
     CDS             complement(27270..27623)
                     /locus_tag="PC479_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2803870.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(27722..28396)
                     /locus_tag="PC479_001318"
     CDS             complement(27722..28396)
                     /locus_tag="PC479_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2803871.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(28401..29099)
                     /locus_tag="PC479_001319"
     CDS             complement(28401..29099)
                     /locus_tag="PC479_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2803872.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(29122..29862)
                     /locus_tag="PC479_001320"
     CDS             complement(29122..29862)
                     /locus_tag="PC479_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2803873.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(30132..31358)
                     /gene="murG"
                     /locus_tag="PC479_001321"
     CDS             complement(30132..31358)
                     /gene="murG"
                     /locus_tag="PC479_001321"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2803874.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(31376..32506)
                     /gene="spoVE"
                     /locus_tag="PC479_001322"
     CDS             complement(31376..32506)
                     /gene="spoVE"
                     /locus_tag="PC479_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2803875.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(32558..33913)
                     /locus_tag="PC479_001323"
     CDS             complement(32558..33913)
                     /locus_tag="PC479_001323"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2803876.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENFMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(33932..34900)
                     /locus_tag="PC479_001324"
     CDS             complement(33932..34900)
                     /locus_tag="PC479_001324"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2803877.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(34941..36314)
                     /locus_tag="PC479_001325"
     CDS             complement(34941..36314)
                     /locus_tag="PC479_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2803878.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(36481..38460)
                     /locus_tag="PC479_001326"
     CDS             complement(36481..38460)
                     /locus_tag="PC479_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2803879.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(38472..38819)
                     /locus_tag="PC479_001327"
     CDS             complement(38472..38819)
                     /locus_tag="PC479_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803880.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(39300..40235)
                     /gene="rsmH"
                     /locus_tag="PC479_001328"
     CDS             complement(39300..40235)
                     /gene="rsmH"
                     /locus_tag="PC479_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2803881.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(40265..41011)
                     /locus_tag="PC479_001329"
     CDS             complement(40265..41011)
                     /locus_tag="PC479_001329"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2803882.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(41405..42691)
                     /locus_tag="PC479_001330"
     CDS             complement(41405..42691)
                     /locus_tag="PC479_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2803883.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(42706..43971)
                     /locus_tag="PC479_001331"
     CDS             complement(42706..43971)
                     /locus_tag="PC479_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2803884.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
BASE COUNT        13639 a         8255 c         4517 g        17672 t
ORIGIN      
        1 ttagatggca taacttttta gatacataaa aaattttata ttaattagat attattaaaa
       61 taaaagcgat acatgaattt ttaaatgtcc atgtatcgct ttatttttgc cataatctta
      121 ataactgatt tttaaagttt attatcttaa caaagttcat tatcttatat aatatttaaa
      181 tgcataggct tctttaattt aaccatctaa atatttaatt aaatattatt atatgtaata
      241 taactaattt ttaaatgaat gtataggagc tggaatcctt cctcctcttt gagtaaataa
      301 ttcagatgaa tttgtattta taggcattac tggtgctcgt cctaaaagtc ctccaaattc
      361 taccatatca cctacatcac atcctggggc tggtataatt ctaacggctg tagtcttatt
      421 attaatcata cctattgctg cctcatcagc tatcatggct ccaagcgtac ctgctgtagt
      481 atttcctggc acagctatca tatcaagacc tacagaacaa atagcagtca ttgcttcaag
      541 cttatctata cttaaagcac ctttaataac agcatcaatc atacctgcat cttcagatac
      601 tggtataaac gctccactaa gtcctccaac atgtgaacat gccattactc cacctttttt
      661 aacagcatca tttaaaagtg ctaatgctgc tgtagtccca tgagtaccaa caacttcaag
      721 acccatttcc tctaatatat ttgctactga atcaccaata gcaggtgtag gagctagtga
      781 taagtctatt atcccaaatg gaacatccaa tctactagaa gcttcctttg ccacaagttg
      841 tccaactctt gtaattttaa atgcagtctt tttaactgtt tctgaaacaa catcaaaagg
      901 ctctcccttt actttctcta aagctctctt aacaacacca gggccactta caccaacatt
      961 tataattcta tcagcctcac caacaccatg gaaagctcct gccataaatg gattatcttc
     1021 tactgcatta caaaatacta ctaattttgc acaaccaaaa ccttttgcat cttttgttaa
     1081 ttctgcagtt tcttttatga tttctcccat ttctttcact gcatccatat ttataccaca
     1141 tcttgtagaa cctacattta ctgaagcaca tactttatta gtacttgcta gagcttttgg
     1201 aattgactta attagtatct tatcaccttt tgtatatccc ttttgaacta atgctgagaa
     1261 tccaccaata aaatctatac ctaaatcctc tgcagcctta tccaatgtct gtgcaaactt
     1321 tacatagtca ttttcatctg ttgcacctgc aataatggaa attggagtta ctgataccct
     1381 tttatttaca attgggatac caaattcacg ctctatatct tttccaacct tcaccaaatc
     1441 tttagcagaa ctcataatct tattgtatat tttttctcta gctttatctc catcagggtc
     1501 tatacaatct aataaagaaa tccccatagt tatagttctt atatccaact gttcttcatc
     1561 tatcatcttt attgtttcaa gtatatttcc tacattcact attatcacct ccccttactg
     1621 tctactacag attatgcatt gaattaaata tttcttctcg ctgaattttt acatccactg
     1681 ataatttctt acctctttca accaagtcct caaccgtttt ctcaaaagaa tttaatgatt
     1741 tctctaaatc aactatcata atcatagtaa aataatcctg taatattttt tgtgatatat
     1801 ctactatgtt gatattttgc ttgtataatt catcactaat tcctgccact atacctggtt
     1861 tatcctttcc aattactgtt aaaattgctt tcataataat atcctccctt atttttttat
     1921 aaatcttata tttttacaaa ttgaactatg ttttaacata tatttcattt atttgttagt
     1981 aaaccacttt ttatgtatat atttaatata ttttaatacc tatacattca tttgtcaatt
     2041 ttttttcaat attctattat ttttatacta tttagaaaat tcatatatta tatatgtttt
     2101 ctcaacaata aatacagttt tcataataat gaaaggtatt acttagcaat cgcatgattt
     2161 tctaaataat acctttctaa aaattatttt tttaatttta tgaatttaat tatacttttt
     2221 ctagtatcag gatttacaat taatatagaa ccaatcataa ttggtgttag tatacaaaat
     2281 aaaaaaataa tatctttttt tagattatat tctttagatt ctaatttata attattattt
     2341 attattccat cataatcact tatatttgac ttatttttat ttgtatcttg atttttgtta
     2401 gaaatacttt ccctataagg aaaagaatgt tcattggtac caaatactaa ttttgagctt
     2461 aaatcataat tatcatcatt ttctgtaatt gaatttttat tgtaaaatcc tttttcttta
     2521 tcagaattat tttctttatt accgtcttta ggtatatcat gaggattttt attttcattg
     2581 ctatcagtac ttgattctac atcatcttta ggagtatttg gttgtttctc atcagaatta
     2641 tcattttcat tattattatt ttcattatta ttgttttgat tgttattact atcattattt
     2701 tcatttccat tattcccatc ttgattgtta tcatcctcat ctctatccgg gtcgtctcta
     2761 cctcctcctc cacggtcacc atcaatatca tcattttccc gttcatcatc tggtgtctct
     2821 tggttgtctc cgcctcccat tataaatcct ggtttaaaaa ctatatttga atacccttct
     2881 tttaatcctc ctaccacttt tactctaaaa caacgcaact taaaatcttc aaaaaaaagt
     2941 atagcttgat ctctaatatc ctcctgttct tcagatatcc aattgattcc atcgcttgaa
     3001 tattcaacaa aaaccttact tgcactatac ggcatatcat aaaataaaac agctacatat
     3061 ccattttttg tattttgaaa ttccagttcc aaattgtcta ttggtttttt ctctataaca
     3121 gatacattca aaataggaac tacatcatca tttattataa ttttttcttt atttaacatt
     3181 tctgttttaa actttcctgt tatattaaaa ttttctccct tttccaactt ttcataataa
     3241 tcagtaaaat cccattctac atctatatca agtcgattac cactagatgt ttctaatacc
     3301 atatatttgg gaaattttat attttctatt ttatcatctt tacttacttt tatttcatca
     3361 ctaaatacac caattcccct atgacaacat ttcacaatat catagccaga atcttcagct
     3421 atagagggta taagctcatt ataatcatca attatataaa ctttcttact ttctgatata
     3481 attgattgtg ccaacttatt attatctttt agtaacccac tttcattgtt gctactatat
     3541 gcttttattg aagtttcttc tatctcaaca tcacctttag aataaacacc tatagcatgt
     3601 tctccattca cttcaatatc cttatttagt attctaatat cattttcagt ataaattcct
     3661 attgcatctt tcccatttgc ctttattttt tcaacagatt cgtaggatga tttaatatgt
     3721 aagtctcctc cttcttcaac gtataaggct gtcccatcag tagcaattat gttgtttata
     3781 ccaatactta tacttccacc attttctata tgtattactc cttcttttcc tccttcacct
     3841 attatattca attcattatc atcaataata aatctcccat gattttttat caatatttta
     3901 tatttgttgg tatctattgt aaaattactg tcataaggaa tataaaatct aaactcttca
     3961 tctattacca tatcacaaga aagagtataa actatttctt ctttattatt tttattttct
     4021 tgaacccacc tttcaaaatc ttcaagacaa ttttcctctt gtccaaaaac tttatgtgga
     4081 aaacttataa aacacaaaac tattatactc ataaaaataa atatctcata ctttttcatc
     4141 ttacactcct agaaattttt tagctttgaa acaatctgtc gaaacataca cttcacccac
     4201 tataattttt ccacaattat caattattgt caatttataa aagtatcttt attaattgtt
     4261 ataaataaaa agctacttac taaagtaaac agacgtttca ctttaataag tagctatatt
     4321 tttataacaa aatagttata atattagaac ttccttcatt tacaattttt tgtaaagtct
     4381 tttgcatttt aactcttatc tcttctggca tagtatataa tttactttgt aattgctctt
     4441 ttactaagtc atgtagtgat ttaccaaaca tatttgattc ccataaatct gctggttgct
     4501 cttcaaatac ttccataaga tattttatca tctcttcacc ttgattttga tttccaacta
     4561 ttggagaaac ctctgtagat atatctgctt ttataatatg aagtgatggt gcattggctt
     4621 ttaacttgat accatattgt tttccttgtt tcattatttc tggttcttcc agactaagtt
     4681 cttctaagga aggagctact actccatatc cttttatctt ggcatctatt aaagcacttt
     4741 ctattttatc atactcattt ttaactttag atagtctagt tatcagacta agcagttgat
     4801 aatctccttc aattttaaat ccactttttt cttcaaggac attatagaat aaatcttgct
     4861 ttgtctgtaa atcaatattt ataacacctt ctcctagctc tacattatca acacctgtat
     4921 cttccaaaaa ctctaactca gaaaatcctt gtataatacc ttctatatcc cttattttac
     4981 ctatatctat aatactttgt tttaatgttg ttattatgct actttttatc caatgatttc
     5041 tttcaagacc ttcaacccat tttggtaagt tgattcttat ttctgtaagt ggaaaatcat
     5101 ataatacagc ttccataacc tcctctatat cttcttcttc catttcaacc acattcattg
     5161 gaagaactgg aacttcatac ttttcttcca attcacttct aagcatactc gtttcctcac
     5221 tttttggact taacgtattt aaaaccactg caaatggttt tttaagattt ttcaattctt
     5281 gtataactct ctcttctggt tctacatagc tttttctgtc aattccagta actgaaccat
     5341 ctgtcaagac aactatccct attgtagaat ggtcttttat aactttttta gttcctatct
     5401 ctgctgcctt ttcaaatgtc atagcttctt gtgaccaagg agtggatact aatctttgtt
     5461 ttccaccttc ctcatgccct aaagctcctt caactatgta tccaacacaa tctaccattc
     5521 ttacttttaa agatactgtg tcttttattt ttatttctac accatctgct ggtacaaatt
     5581 ttggttctac tgtcattatc gttttacctg aaccactttg gggtatctca tctcttgttc
     5641 tatccttttt aaattcattg tctatattag gtataaccaa cttttccata aattttctta
     5701 taaaagttga ttttcctgtt cttacaggtc caactactcc aatatatata tccccctgag
     5761 tccttttgga tatatcttcg tatatgttat tattcatatt attcctccta tacatctaat
     5821 cttattaaat tatatttaga gttaagggtt aatattctta aaatagaaaa ataaagacca
     5881 gcaatattaa tttaatttat atatagaaat ttgtaaattt caatgtaaat taaattaata
     5941 ttgctggtcc aaatagtagt gtattatatt aactgacttt agtagtattt tcatttgaat
     6001 tattattcat ctcaataagt tctttacgtc tttcttttgt catgttcatc catataaatg
     6061 taaacaccaa tccaattatg gctactatag taagaactat gaagtagtac ctatatccag
     6121 ttacacccgg aaatgcatcc aatatcttcc ctgctgctaa aggacaaagt acttctggaa
     6181 gatatcctaa cgtagcaact attcctatag cagttcctga tattgataat gaataattac
     6241 cttcttcaag taacgaatag tgtaaacctt gaattgcata catagataca tatattgcaa
     6301 cacatccaat caatagtata taaatcatag atgattttgt tggtgtaaat attacaccta
     6361 aaagacctgc aatcataagt ataaatccat aagatataat ctttgatgaa ccaactttat
     6421 ctccaagtaa tccagaagca gctgatgcta ctggtctaca atactgagct aatattgaga
     6481 ttgcagatgc aaacaccaca gtagctccaa atgcttcagt tgaataaggc gtaaaataat
     6541 aaaatgacat attagctgaa tatgaacaaa acattattat tgttattatc catgtatgag
     6601 gcatttttat tactgcaaaa aaggcttctt tatcaaattt tgctgatacc tctttttcat
     6661 tatcctttaa tttgaaaaat actaatactc caactattac atttattgct gagtataata
     6721 ctataatagc tgtaagacca gattcatcac ttacttttga tgaaatatac ccaaatagta
     6781 tcaatactag agatagatga attgcattgg taattccacg accactttcc ataaatccaa
     6841 aagcttttcc ttgttcattt tcagatgcta acattctaac agcttttaca agagctggcc
     6901 aaaaagtaag tattgttgtt attccccata aggcatggat taaaaacaca acaatatatg
     6961 gaggataaag tgttaataca agaccagata ttcctgtaat aattaaagaa cctgaaagca
     7021 attttctggc tgaaatacgg tctgctaaaa aaccaccaaa aaggtatgat accattgcaa
     7081 gtagaccata aatgcttcct aagcttccca tttgtgtatt tgtaagatta aatgcttgtg
     7141 caaaagtatc ataataataa ttctttaaat atggtaaagc atatataaat gctcctgcta
     7201 caaagagtat tatcaagact ttgaaatttt tccaaatgct tggtttattt acttcaactg
     7261 acatacttcc tccttatgta aatacctgta aattttcata ataattaatt attgcttatt
     7321 ttattgttta ttcaatattt aggaatattt attaatatat taggttaatc tatcaataat
     7381 ttatggtgag ttatgagcta aaagtaaact cttttagctc accataataa ttatattttt
     7441 atttatacta acatccagag ttattagata tctttttttc atagttcttc catagcatta
     7501 aacctgttat tataacaaaa agacctcctc ctatcatttc aatctcatag attatattat
     7561 cttttaaagt ctccataact ggtgccgttg agaaaagtat gccaactaca gttactacaa
     7621 gtaccattct ggcaatattg atacaaactg aatcactttt tgcaattgaa tatggtcttt
     7681 cttcatttgg tcttgtcttt ctaagtctaa tataagatgt aaaaagcaag acatatggaa
     7741 ataatgctgt taatgctgtc atagtaacaa gaacattgta tatattatct actgatggta
     7801 ataagtttgt tcctacaaga agtagactca ctattatagc ctgaactatt actgcatttg
     7861 atggtatatt gtatttattt gtaacagtca attgcttagg aaatacacct tctcttacat
     7921 ttccaaaaag catctttact ggacctgcta tataaagtat taatgctcct agtactgata
     7981 aagttattcc aaatgctact attcttataa accaacttcc tatacctaaa tcttgtgcaa
     8041 ctttagctaa tgcatctagt atccctgtag atgctgcaat tttatctgga ggaagtatca
     8101 ttgttattgc tactgaacct aagatataga ttcctcctat taaaacagcc acagttaata
     8161 tagctttagg aaagtttttc ttagcattat ccatttcggt tatgaaatta gcagtcgttt
     8221 cagctcctga taatgcaaat attatagcag atatagaaac taatgaattg gcatctattt
     8281 ttggtattat attttggatt gtatatatcg atgctgatgg agcttttttg agaataacta
     8341 cagacataaa tgccatcact ataagtaata tagcaggaat tgtagaacct aatgctcctg
     8401 tattcgtaaa aaattttcca aatgccatcc cacgagtact tactaaggac aacagccaaa
     8461 atattatgag tgaaagtgct aaaataaaca tcttattgtt tgataaatct ggatttccaa
     8521 gcatatatgc aatatttatc gctaaaaaag tcaagaaaga agaataccaa aatatctttg
     8581 atgtccaatt tagccatgac accataaatc cccacttctc tccataagct tgtttaaccc
     8641 attctccaag tccaccatct tttggatatg ttgcagccaa ctctgcacat ataagtgatg
     8701 caggaacaaa gaatataaaa gcaaacaata accaaactgg tatagaacca agacccaatc
     8761 caaaactaga ggcagtagat ttagctatcc atctaatccc aaatagtgct gatatattca
     8821 taaatactaa atcccaaaat ccaattttct ttttcactgt aattcctcca atttcaaatt
     8881 tattcagact catacacaat gtattgactt aaatatcaag tataagttct tcaacacttc
     8941 ttggataatt agttataatt gtagcaccat tcttacctac tattgctgta tctgagtgtc
     9001 taaatcctcc aactccaggt atatatatcc ctggctccat agaaaatacc attccttctt
     9061 ctaatattaa ttcattgtca aatcttaaat atggctcttc atgttctgat aaacctaaac
     9121 catgaccaat tctatgattt gaatataact ctagaccata ctttgcaaca acagctcttg
     9181 ctgcttcatc caccattctt gctggtatac cagcttttat tgtatctaat ccagcttgtt
     9241 gagcctctac tgcaatctta aacacttctt tttgtctttc tgttggtttg cctataataa
     9301 aagttctttc attttctgct ctatagtttt cataccaaac ctgtctacta tgtattacta
     9361 tatctcctct ttgaagtatt ctagtattgg aatctaaatg aggttgtgct gttctatcaa
     9421 tccctgaaca agtccagttt gcaaatccta tatatgtatc aggataattt tcagaaacat
     9481 attttagtaa agcattatcc cctgcaacgt caaactccaa ttcactcatc ccaactctaa
     9541 cattttctaa agaccctttt attcctatat cagataggta acctgctatc ttcaaaaatt
     9601 ctatttcttt tgcatctttt acatatctca tttcaaatat tttacttcca acatctttga
     9661 tagcaaatga cttatcagtt atatactctg aaaagcttgc agatactata tctttttcta
     9721 ttccaacagt tgttccttta gggtatttat ttaaaattat atctaagtaa tgtttatgtg
     9781 atattccatg ttctttcatt tctggtattt cataatacac atatacatta tcaacttttg
     9841 caacttcttt agcatgtaat tcttctaatc caggtactat taattctatt ttatcttgag
     9901 ttactactgt tacaattggt ctagaataag ttattgcttt aaatccacta atataaaatt
     9961 gattgtctgg ttcaaatatt aaagcaattt caatgctttt ttcattcatg tactttctca
    10021 atctttctag tctcatatta tttgacataa tgcacctctt tcttaatatt cagataattt
    10081 attcaataca acaaggatga atatattatt ttaaaattct taatattttt agtacttgtc
    10141 gtatttgtat gtaaattaat tttatttgtg attattttat cataatcatg attattttgt
    10201 caatcatgat tttattttaa aaaatttttt atattctagt gattttaaat tatctaaaat
    10261 ataacgagta tacattgtat tttgtaatat tattcttgat ttatggtaag tttaatgata
    10321 aaatcacgat tatataaact atattgatta tttgtaggtg aataaaatga caaataaaga
    10381 attacaaaag aaaaggatat tgatgtattt catagaagct gctcaaaata ttatggaaaa
    10441 cgaaggtata gaaaatatta cacttcgtaa ggttgcagat atggctggct ataatagttc
    10501 aactctatat aaatacttta aaaatttaga ccatttaata tcatttgcat ctataaaata
    10561 ttttaaagat tataacttga atatttcaag atgtatagaa aatgtcaatg atgaatataa
    10621 gaaatatata attatgtgga aattattttg taagcattct tttgataatg ctcaagcttt
    10681 ttaccaggta ttttttaact tgtctagtga tgaacttagc tatataacaa aacaatatta
    10741 tgatatgttt ccagaagatt taggtattca tgatagtgac atatctttaa tgattacagg
    10801 agaaagtata aaggaaagaa ataagatact attaaacaat cttgtagata ctggatatat
    10861 accagctaaa gatttagaca taatcaatga tataattatt tctttttatc aaaatctatt
    10921 attcttaaaa aagaacacag gaaaaaattc tgatgatgat gaattaacta tgaaaatgat
    10981 ttcttctatt gaatacataa taaaacatgc taaataaaat taaaatatgg ctgtctcaaa
    11041 atagagataa gcctttttta ttgttgaaat aataaaaata tatttaattt catataaaaa
    11101 agagtgcctc gtaaactaaa aagttcattt tgagacaccc ctaaaataaa tttattattt
    11161 attatacaat agtttatata tcatcaccta aaactacttc ttccatttca tgctttttac
    11221 ttctcatcat tagttctatt ccaacttctt taggatttga acctttcttt attacagaat
    11281 atatagcatt agttataggc atatctatat ctaatttttc agcaacatca tgtgctactt
    11341 cagtagcagt tatgccttct acaaccatct ttacttcttt taaagtatct tctaaactca
    11401 tccctttacc tatgagtata ccagctcttc tatttctact atgcatactt gtacaagtaa
    11461 ctattaaatc tccaatacct gaaagacctg caaaggtaga catattagct cccatagcaa
    11521 ttccaagtct gctcatttca ctaatacctc ttgtcataag ggcagctttt gcattatcac
    11581 cataccctaa tccgtcacat atcccagctc caaatgctat aatatttttt aatgctcctc
    11641 caagttctac tccaactatg tctggattcg tataaactct aagtttaggg ctcataaata
    11701 aatcttgtat catctgtgct atttttaaat ctttagaagc aactactacg gtagtaggaa
    11761 tatcccttgc tacttcttca gcatgagatg gcccagatag tattacatat ggattttgtg
    11821 gtaattcttc ttcacagact tgagataatc taagcccagt ccctttttca agacctttag
    11881 ctacattaac aataacttgt ccttctttta taaatggttt tattttttgg caagtacttc
    11941 ttacagcctg tgatggaact gctaaaacta ttaccttaca atcttttata gttttttcta
    12001 tatcagtact tatagttata ttatttggaa ataatactcc tggtaaatag tctgtatttt
    12061 ctttagttct gtttattttt ttagcttgtt cctcttttcg agtccacatg ctcacatcat
    12121 tacctttttt tgcaagccct aaagctaatg cacttcccca gcttccagta cctaatacac
    12181 atactttctc catgtttaac accacctgag tataatatat ttactattct ttcttttgac
    12241 caatttttct ctctgtccca tttaataatc gctttatatt tgctctgtga ttatatatta
    12301 ctgatgcagt taagaacaga gctactatta aaccagcttt attttttacc ataatcataa
    12361 atattggtga acatcctatt cctacaacag aacccaatga aacatacttt gttatagcta
    12421 ccaccaatat aaatactgct agacacatta gtgttattac tggatttaca gctagcattg
    12481 cccctagtga agttgcaaca cctttgcctc ctctaaatcc taaaaccgct ggccaattat
    12541 gacctgctac aacacatata actgccagat atccagctaa aagagtatct attccaacca
    12601 atcttgctgc aaattctgat attaaaacag ccattacacc tttaagaaca tcacctaaaa
    12661 atgtcattgc tcctgctctt tttccaagtg tcctaagtac atttgtcgaa cctgcattac
    12721 cagaaccttg agtccttata tcaaccccag caattcgctt tgcaactatg tatgaggtag
    12781 atatatttcc taaaagatat gcaactacag caataattat ataactaaat atctccactg
    12841 aatttccccc ctttagttat attttaagat atttattttt tatttttttg tctatactca
    12901 aactttatgg aagttccttc aaatccaaaa ttttctctta tcttattctc aaggtatctt
    12961 tgatatgaga agtgagttaa atccttatca tttataaaca atgtaatttt aggaggtcta
    13021 atgtcagttt gagtcccata gtaaattttt aatcttctac ctttgtcaga tggtggttga
    13081 tttaacatta cagcttctcc aattacttca ttcagtgctg aagttgatat tctcttagaa
    13141 tgttcatttg acacatattc aactgtatct agtatcttgt tcattctttg attagttttt
    13201 gcagaaacaa atacgattgg agcatacatc ataaatggaa atttttcttt tatatctttt
    13261 gtgtaattgc tcatagtctt attatctttc tctattaaat cccatttatt gattacaaat
    13321 atgcatcctt ttccttcatc atgtgctata cctgcaacct ttgtatcttg ttctgttaca
    13381 ccttctaagg catctattac tatcaatact acatctgctc tatctacagc actcatagcc
    13441 cttattacac tgtatttttc tattgtctca tatattttac tttttcttct aagccctgct
    13501 gtatctatta gaagaaattt ctgtccattt ttttcaaaat aagtatcaat tgcgtctcta
    13561 gtagttcctg caattggact tacaattact ctttcttctc caagtatctt attgagtata
    13621 gaactttttc ctgcatttgg tttacctgtt atagctactc ttattatatc ttcttcatac
    13681 tctgtatcta atcctgctgg aaaattttca actatctcat ctaataaatc tccaaatccc
    13741 atactattgg ctcctgaaac tgcaaaaggc gttccaaatc ccaactcata aaaatcgtat
    13801 atattatcaa attgactttg actatcaatc ttattaacca ctaagattac tggttttttt
    13861 gtctttctaa gcaattgtgc aatttcttta tcagcagctg ttattccagc tttaccatca
    13921 actataaaaa gtattacatg tgacatgtcc attgcaagca tggcttgatt tctcatttga
    13981 gataatatta tatcttcgct atcaggttca attcctcctg tatccactaa tgtaaaatat
    14041 ttatctaacc attctacttc agcaaatatt ctatctcttg taactccagg tgtattttca
    14101 actattgata tcctttttcc tgcaaattta ttaaatattg tagacttacc aacgtttggt
    14161 ctcccaacta cagctacaac tggcctactt atactcattt tatttctcct tccatctttt
    14221 gttttctatt ttaaaatttt atccacaaaa tcttttcctt cattcaaaca agggataatt
    14281 tttattctca taagttcttc taattgattt aattcaatat tatctaagaa aatttcttca
    14341 tctgctttta gcatacttct tgttatataa agagcttctc ctaattcttt atcttcaagt
    14401 tgttctttta aatccttagc tgttaaaaga cctgatacag ttattgtctc tccaaaaaac
    14461 ttgtttttga tttcatatac atttacttga agatttttaa acttgtccat catagcatct
    14521 gccatactct gtataaattc atatgcagaa tgaccagtag ctattgatac tttcttctct
    14581 ctttttagaa tcttctcagg caatatattt agatactctt caatctctgt ttttaactta
    14641 cgtatcatac ctacaccatc ttcaaactgt aaaaaacctt catattcttc atatccaggc
    14701 aattcactat tagatagtat ataaaattca tctgataaaa atgcaaatcg agtacctaat
    14761 ttttcaagat atttctgttg tatttgatgt atttgctcta tagttttact tgcgctctta
    14821 tcattaaata tatttaattc tactagattt tctctatgct ttgtaatccc aactggaact
    14881 atcgcaacac tgtttacata cggataaagt tgagctagtt cttttattgt tctatccaat
    14941 tcctttccat catttatacc tggacataaa acaatttgac agttcatttc tatatgagca
    15001 tctgccaatc tcttcattat accatataat tttcctgcaa atttattgct tatcattttt
    15061 tgtcttaact ctggattagt tgtatgcaca gatatattta taggacttat tctatatttt
    15121 ataatattat ttatatcttc ttcactcata tttgtaagtg tgacaaaatt tccttgtaaa
    15181 aaagataatc tcgaatcatc atctttaaaa taaagagttt ctctcatacc ttctggcaat
    15241 tggtctataa aacaaaacat acacttattt ctacagcttt tagcttggtc aattatcgga
    15301 tttgtaaatt caacacccaa atcttcatca tagtcttttt ctatctcgta tatgtagact
    15361 tcgccatctt ttttttgtat ttctacatct aaatattcat cacttagtaa gaatctatac
    15421 tctattatat catgtattgg ctgttcattc acagatataa gtaaatctcc tacctctata
    15481 cccatttctt ctgcaatact atctttataa accttactta taatattatt tacttttttt
    15541 acatttactt ccaatatttt caccactctt ttgtattcat caatgcttat tataatgtat
    15601 tttaatatgc aatttattta tttattatat tttatgatag catttttaag catttcatgt
    15661 cataataaca cattttaatt ctaatagtca atataagtaa atagctataa aatagattag
    15721 aacaaataat attcaaattt aagtttctta aaaatttaaa tattatttgt tcttaaaaaa
    15781 attttataaa tttaatgttt tactatgatt aacgtcccat ttgtcatatc atgaaccatt
    15841 ccacctacac actttgacat caacaatgcc ttattttgta agtctttctc atcatttgca
    15901 taaaatattg gacaacctcc accatttttc atattcttat caagtgtaac tatagctagg
    15961 gtatattcat ttaaacctat atccattccc atctcatgca cttcctttct ttcctatttt
    16021 ataatttttt agtgataagt tttttccttt agagcttgaa agtattggac atgactttac
    16081 agcttccatt actttttcca cattcttatc tactggtaaa taagcaatca ttatactttc
    16141 atcctcttta tttcttctag gtaatggtac gaatgaaggt tctccataat ctccatataa
    16201 acctaacctt gaatatatat tgtatactat tgcttgtctt tgaccggagt caaataatat
    16261 tcctgcatta gcataatcct tatttttagg aattatttgt attcctacac ctctattaat
    16321 atatacttct ctatcgcttt ttagacctat atttgttata ccttttaaat ctcccacctt
    16381 tagtatagat tcgtccacaa aactaatttc agcaggcatt acatcagcta tgtctccaat
    16441 actttttcta gtaagaagtt tttttaatat aaaggctaac actaatccac aaattgaact
    16501 tattaccaaa ctcatccctt cacttatttt cgtttctgca agtattacat aatacaatcc
    16561 tattgttaaa aaagatgtaa ctatacacat ataatttctt acttcatagg ttctagctat
    16621 ctcttctatg aatgcagctc ctctctttac aagttgaact tcctctaaat tttgcaatgt
    16681 atttcttcta tttgctctca cttgtctaaa ttgttctgct gctagagata aaaaagttat
    16741 agatgtataa gaacgattta taagagctgg aaccaataat gctcctaatg aagaagcgac
    16801 gaatgctaat ataagttgag atagtaaaac atttggttgc gttggatact gtttttggtc
    16861 taaattcaac attataattc tagatgttat accaataata acagcaacaa aaaatactct
    16921 atccattaaa tcactcctaa aatatttaat ttgaaatttt acatgtaaat ctacaaagtc
    16981 tttctagaaa aatcactctg ctttcctttt gatgtttcta tgataggtgt acttttcact
    17041 acatctatca tgacatctat atctttgagt attggcatat atggtataac cacagtactt
    17101 ttttctaaat tggtcttaga tatagcaaca atatcaacct catctacatc tctatctatc
    17161 cccatatgaa taaatagatt atgtattata gcttgtcttt gccctatatc atttactatt
    17221 ccaaatgcac ctaaatctct tggtataact tctatggcca gacattcatt tctatacttt
    17281 tctcttgtac tttctagacc tatgttagtt attattacac catttaccat taaaataggt
    17341 ccttcaaaac ttatttttgc aggaactata tctgctatat ctgctatact gttccttctt
    17401 aaaaatcttc taaaaatcaa accaacaata gctcctgaca caatagctag gattgtgcaa
    17461 taaaaaaagc taaggccata ctttcttgca aaaacgatat aaactatcga agataccagt
    17521 gcagaaaaaa gacttatata acttctagat tcatacgtag atgctatttc ttcaacgtaa
    17581 gcgtctcctt ttggtactaa ttcttcatta tcgatattct ttagtgttat tctttcttgt
    17641 tctgcaagac cttgaaactg ttgtattgct actgcaaaaa atgtcaaggc tgcaaactcc
    17701 ttatctataa gtgcaggtaa tgctatagca cctaacgaag ctgaaagccc agatataatt
    17761 atctgttcta aataatcttg tggtcttgat gggtattgtt tgtctgtaac tcttaaaact
    17821 aatcctctac ataatattcc tacaactaat gcaactataa aagagtgtct aaataaattt
    17881 gcattcaata aactttcttc cattttctca gctccttctc ttatatagtt tgtgcaaaaa
    17941 acaaaaaaaa ttcccttcta tttaaggaaa tttttttgta atattgtaac tataaattcg
    18001 ctaccaatgc caagttcact ttttactttt atacatccat ttagtgattt gactatatgc
    18061 tttgttatag ccaatcctaa acccgttcct cctacatccc tacttcttgc tttatctacc
    18121 ctataaaatc gttcaaatat gcgttctaca tcttcttttg gtatccctac accattgtct
    18181 ataactttaa ttacgatttt ttcttcatct tttaacacta caacttgtac aatcccatta
    18241 tcttgggtat actttatagc attatcaact aggtttaaga acacttgctt tatatagtct
    18301 ttattggata gtatacttat tgatttatca tcacatttat ataaaacttc tatatttttt
    18361 tcactagctg aatgttgagt tatttcatat acctctttaa atacatcata tactacaaca
    18421 ttttccataa gaagagtatc tttattttct acaaatgata acagcaatat atcatcaatc
    18481 aaccttttta atctatctga ttcgctttct attatttcta agaatctatt tctagtattc
    18541 acatctatat tttcatttga ctttagtgtt tcaacaaacc cacttataga tgtaagtggt
    18601 gttttaagtt catgacttac atttgcaaca aaatcacttc tcatattttc aagtttaact
    18661 tttttagtaa tatcttctat atttactatc gaacctatta ttacgttgtt agtattctga
    18721 agataaactg ggtctacttt tatcttatat acgatatcat ctttagtagt aatattagca
    18781 gtttcattat ctttagaacc aataaattga agtatttgtt ttaatatttc ttcttctttt
    18841 attacaacat taatattatg accttcaact aattgatttg gttcactctt tatcatttct
    18901 tttgcttcat catttatcag aagtatattt ccatttacat ctatagccaa aattccatga
    18961 gatatactct ttaatataga ccttaactgt aaatgtttat actgtacttc ttctaaggtt
    19021 ttatccatat tctttatcat atgattaaaa tttctagcta attcgccaag ttctcctttg
    19081 gctgatatgt taagcctagc atgatattca ttattactta ctttttttga gacgtatata
    19141 aactctttta aatatttcct taatgttatt gtgtatctta cagctaatat aacaatggct
    19201 atggacaaca taacaataaa aaaatatata ctatttaaat cttccacaag ataacactcc
    19261 taaaatataa cctttttaat ctattttgta tcctacacct cttatagtct ctatatattt
    19321 ttccgattta tcttcatctt ctattttttt tcttaaatat cttatatgaa catctactgt
    19381 tctagtttct ccatagtatt cataccccca tattttatcc aaaagataat ttcttgaaag
    19441 tacttttcct ttattttgaa ttaataattt taacaattca aattccttaa gagttaactc
    19501 tatcttttca gagcctttgg taacttcatg ttttgataaa tctagtttta aattacctgt
    19561 agtaagtata ttgttttctt cacctaagct agtcaaatta tatctcctta aaacagaact
    19621 aatcctagct aacaactctt ttatactaaa tggcttagtt atatagtcat cagcacctat
    19681 ttcaagccct tcaaccttgt cattttccat atttttagca gtaagcatta taacaggaat
    19741 attttttaag tccttatcac ttcttatttt tctcaaaact tctattccac ttatatttgg
    19801 taacatccaa tctaaaagta ttaaatctgg tttaatttcc ttagccttta taaatccgtc
    19861 aaatccatca tatgaataac taacttcgta atttgacacc tccaaattga attttaatag
    19921 ctcaactata tgcatttcat cgtctataac aagcacttta gtattcatta aaaagctcct
    19981 ctctatttta tctgtagtac cgttcctatt cgttctgatg ccttattatg ttttctataa
    20041 gagtggaatt tatctgctct acaactagtg cataaattta aatttattat attttctttt
    20101 ttaactccac tacatctaag catatactca ttaattttcc ataaatctaa atagtaactt
    20161 ccctctttta ttatatagaa cttttcatca ttatttgtaa gaattgtgtt aaatttttct
    20221 actaaatctt cagacacttc ataacaacat ggtccaattg atggcccaat tatacatact
    20281 aaatcttcag gaactgtttt atagcattca gacattaatt ttattgtttt atgacctata
    20341 ttactaaaag tccctctcca acctgcatga gccagtccta tggctttatt tttagcatct
    20401 ataattgata taggtacaca gtctgctgta aacagtaaaa gtggtacttt ttccaagttt
    20461 gttataagtg catctccatc tcttcttttc cctatagtat ctttattgac tatattaact
    20521 atatctgaat gtatttgaga attactagtt agattttcaa atgtcaaatc acattcttta
    20581 catactttta acatatcttc tctatttttt gcatctatat tcttacatgt tataactaga
    20641 tttacagaat ttaatttttc atcaagttct tcacaattac cacttaaatt aacatactct
    20701 ttaatatctt gtacattttt tatatttata taatctttca tcatttgtct cctttttcta
    20761 tcagtatact tttaagttca tttacaaaag tatttatatc tttaaattgt ctatacacag
    20821 aagcaaatct tacataggca acctcatcta actctttcaa tttatccatt accatctcac
    20881 cgattttttt ggtttcaatt tcttctataa aacatttatt tatttcattt tcaatatgta
    20941 caacaatact ttcaatctcg tcaatagaaa caggtctttt ttcacatgct tttagcaatc
    21001 catattttat cttttctcta tcaaaatatt ctctactatt atccttttta atgaccatta
    21061 aaggagttgt ttctattttc tcatacgtag taaacctctt tttacatgat tcacactcac
    21121 gtcttcttct gatagatttt aaatctgtat gtctagaatc tattaccttt gattctttat
    21181 aattacaata aggacattgc atctatattc ctcctttgtt taactatatc tcaaactcat
    21241 ctaagcttaa ttctgaacct atatttacta ttattgtatc acaacctatt ttaagtatat
    21301 cattccaaaa tataacttct tcatcccctc ctcttgaaaa aaaacttcta cttccatctc
    21361 caaaaatggt aaatgagact accttacctt cgtgaatatc catgtctatg tctattataa
    21421 accccatcct ttttccattt tttacattta taatttcttt ttccattata tcagaaactc
    21481 taatcatatt gtactcaccc tttctttaaa aataaatatg tatcgaagta aaaaaaaatg
    21541 acctatatag gtcatttaaa ttaaaataat gtttacagtc catggattac ttatacatat
    21601 tttctcatat tttttaaagc atttttttca attcttgata cttgtgcttg agatataccg
    21661 atttcatcag caacttctat ctgagttctt cctttgtaaa accttaaatc taataccaac
    21721 ttttctctac tatttaattt cttaatagct tctttaagtg atatttcttg caaccaattt
    21781 tcatcagtat cttttttatc ttgaacctga tccataacga atattgcatc tccattgtct
    21841 tgatatactg ggtcaaacag agatattggg tcttgtatgg catcaagtgc cattacaact
    21901 gattctactt ctaattctaa ttcctttgct atttctgata ctgtaggttc tttagaattt
    21961 gttctaatta atctttctct aacttgtagt gccttatatg ctatatcttt taatgaccta
    22021 cttactctaa ttgggttatt gtctcttaga taccttctaa tttctcctat aatcatagga
    22081 acagcataag ttgaaaacct tacattttgg cttaaatcaa agttatctat agcttttata
    22141 agaccaatac aacctatttg aaataaatcg tctatatttt ctcctctgtt gttaaacttt
    22201 tttattacac tcaagactaa tcttaaattc cctcttacaa actgttgtct ggcttcttca
    22261 tcaccatttt ttatttgtaa taaaagttcc ttcatttgct tatttttaag aactggaagt
    22321 tctgatgtat taacaccaca tatttcaacc ttattaactt gcatatattt tcagtcctct
    22381 ctcaaaagta ttcatctatt gaaattattt acatttccaa tttttttata ctttttaaga
    22441 agaattaatt tagcattata caaatttttt catttctttt tgcaaccttg agattatttt
    22501 cttttcaagt ctagatatgt aggactgtga tattccaagc attccagcaa cttctttttg
    22561 tgttttttca atacctttat caataagacc aaatcttaat tccataattt gcttttctct
    22621 gtcacttaat ctgtcaagtg ccataaccaa caaatctcta tctatttctt cttcaattat
    22681 tttgtatatt tcatcatttt ctgtacccaa tacatcagat aataaaagtt cgtttccatc
    22741 taaatctata tttaaaggtt catcaaatga tacttctgtt ttctttttat tattttttct
    22801 taagtacatt aatatttcat tttctataca tctcgaagca taagttgcca attttatatt
    22861 tttattcagt ttaaaagtat taactgcttt tatcaaacct atagttccta ttgaaatcaa
    22921 atcttctaca tctatacctg tattttcaaa ttttctcgat atgtagacta ctaatctaag
    22981 atttctttct attaaaattg actttacact ttcatctgtt tctagctttt gtaaaagctc
    23041 catttcttct tctggtttca atggaggagg aagtatattt gcccctccca tatagtatat
    23101 tccctttggc tttattattt ttaatttaat acctaacata gtaataaagc ttattattct
    23161 ttctttcaat cgtaacattc cttgtccccc ttaaattttc acctaaaatc caggcaatat
    23221 atttggattc attattgctc catattctct atcattaaaa tttgaaatac caagtattat
    23281 atttcctatc ttttgctcat ctacttctat atagtcagct tttaagccta ttattatgct
    23341 tgtcttatta cttccagcat gtttataagg tattattcta actctacctg aagttttatc
    23401 atctaaactt cctataattt cttgtgcttt cttcatatct atatcttcat aattaatatt
    23461 taatagtttt tctggaataa atccttctaa cacactagac ttaactatta ttacatcact
    23521 ttgacttatt gggtctttta acaaattacc actatctact aaagccttac agcaaaaact
    23581 tttttctagt aagtttatat ttatagtctt tgtaagctcc ttaatgtact taagcatttt
    23641 caaatcttta taaatgtatt ttaataattc acaactcaca taagtacatg caattaagaa
    23701 agatattttt aagtgactta ttcctgtaaa ataaattata aaataagtgc ttccagaaat
    23761 aaaaatgttt actagataaa aaactaacgc tacatgaaca taatcttttt tgctcttact
    23821 tctaaatgaa attaaagtta ttatactcat gattattatt ttaaatggta cagtaaatag
    23881 tatattcatt gatggataca aataagcaac agaataggca cctcctaaac tagcccccaa
    23941 taacttattt tttttaggat tatatctttt agtaagaata gaagtacaac ttataataat
    24001 ataatttatt actaggtttt ctataacata gtactcaata tacaccatgt atcccccctt
    24061 aaaaccttta aacatattat aagatagtat gtacaaaatt ttatgtcact tttgatggtc
    24121 gaatccaaaa caaattctat ttaaaaagaa caatgatata tgtcttatac tttatgtcgt
    24181 caaaagatat ttttttaata ccaaacaaat agactttttt gtagaatcca atatatctag
    24241 gtagaagtta ttcactctac ttttggtata tttacaaaaa aagactacat aatttaataa
    24301 tatatcaagt aaatctttta cttatataaa atcaaattat atagtctgta aattcaaata
    24361 taaattattg tgctagtaat ccatttgcat attttgcaga ttcatttagt gcttgttctg
    24421 gagttatgtc tccagctatc atcatttgaa gttgtttcca taaatattca tatatctcta
    24481 ctccatgagg tccaactgtc aatggtctat acatatgttt atctcttgtc accatttctt
    24541 caaatcttgg gtctccttgg tatttttcac ctttagcaac tggagctcta ggtatttctt
    24601 tgtggaaagc ttccattgat tctttgctta gcatatactc aagaagttta aatccaagtt
    24661 ctttattttt acatgctgac attaatgtta aatcatctac tgaagcaaaa cttccaactg
    24721 tcttatcttc taatgatgtt acatatcccc agtttaaatc cgggaatgat ttatcaaaag
    24781 tacttgtagc agcagaggat agcattatag taaatgcagc ttttcctggt ccaaatacac
    24841 tttcaatcat ttcattacta tcttttgata aagcatcttc tggaatatac tttttcatat
    24901 catttataaa gtttgcagct ttcaagcctt ctgcattatt aaatttaact gattttaaat
    24961 catcattata tatatctcca ccagcttgcc ataaatacgg ataccagttc cagtttaagc
    25021 ttccaaatac tttagaaccc caaccttgtg caagacccca ctggtcgatt tttccatctc
    25081 catcagtatc ttttgttgct ttttcacaga ttcttttaaa atcatcccat gtttttggag
    25141 gtttttctcc taatttttct agtatatctt tattgtagta caatacgcct ggattagcag
    25201 cttctatagg taaaccataa ataccaccca tcatttttgc atcatctaag tatagataat
    25261 tatctgaagt tcctgatttt tctaaataag gtgttaaatc ttctaccgca cccatttcta
    25321 taaattgagg aaacatctct gcatacatgt atcctatatc tggaccttca cctgcactta
    25381 ttgctgttgc atatttttct gcgtagttat cccatggtac tatctgaaat tcaatttcac
    25441 agttattttc tttttcaaat ttttcaaata tcggcttcca tgcatctgca tcatggtctg
    25501 ttaatggtgc agaccataca actaattttt ctttaccctc tttgccttta gaatcttctt
    25561 tttttgaaca tcctgcaaga attgatactg atagaatccc taccataact aaagaaagaa
    25621 ccttttttag tttaatcatg cttaatcccc tttcagactt taatttgtct ttttcctcaa
    25681 atgtttacat ttaattcaga tttaatatta acaaataaga ttactcttgt ctaatcataa
    25741 aaaactatac taactcctat aactatattt tttttctata cctattagat ttaattcata
    25801 ctaaaaactt actttttaaa ttttatcaat atttaaaaat ttttttaagt ctagccacaa
    25861 actaactatc taaattgagc aaaataaaaa gaagtctatt gacttcttta taagcttaca
    25921 tattatcttc ttcttcttag gaatgtaggt atctccatgc tctcatcatc atcttcttca
    25981 attatacttg cctttttagg aggctctatt ttttcctcag caattacttc ttcttcaatt
    26041 tcttttacag cttgttttac tgttgtattt aatgaagaac gtatgcttga tttaggttta
    26101 gtatctaaat ctaaggcacc attttgtaat ccttcaaatc ctgtcgcgat tactgtaatc
    26161 attatttcat cacctaaatc ttcttttata gaagctccaa atataacatt tgcttctggg
    26221 tcacaggatt ccataactaa agttgatgcc tcattaactt caaataatcc taagttagga
    26281 ccacctgtaa cattaagaag tactccttta gctccttgta ttgatgtttc tagaagtgga
    26341 gattgaatag cttgtcttgc agcatcaatt gctctagttt caccactagc actacctata
    26401 cccatatgag caagtccctt gtctttcatt atagtagtaa catctgcaaa gtccaagttt
    26461 attaaccctt caactgctat aaggtctgaa attgattgta taccttgttt taatacatca
    26521 tctgcaactg caaaagcatc taacattgat gtattttttt gtactatctg taaaagtcta
    26581 tcatttggta tcgttataag agtatctact tttgatttaa gttctgctat tccaccttca
    26641 gcgttcttca ttcttatctt accttcaaat gcaaaaggtt tggtaactac acctactgta
    26701 agtatcccca tttcttttgc caagcctgca accactggag cagctcctgt accagtacca
    26761 ccgcccattc cggctgttac aaagaccata tcagctcctt gaagtaactt tactatttca
    26821 tctttactct cctcagcagc tcttttccct acttctggat ttgctcctgc tccaagtcct
    26881 ctagttaatt tttctcctat ttgaacttta tattcagctt ttgatgtata taaagcttgt
    26941 ttatctgtat ttactgatat aaattcaacg ccttttagtt gagcttctac cattctattg
    27001 acagcattgt ttccaccgcc acctacacct attactttaa tttgtgcaca ttcttctaat
    27061 tctacgtcaa agtttagcat cataaacccc cttctctcat gattcaaagt tatcgtatgt
    27121 attaattata tcattttctt cttttatttt gttaaccttt aagtaacata ttccacaaaa
    27181 acgtaaattt tcctttttaa atttcttact tatattgtca aatattactc tttaagtata
    27241 aaataattta cgtatatatt aaatgctctt taatttttac gttttttgtt cataatatta
    27301 ctaataatat atcgtctaat tatagataag ttattaaaca atctcatacc aaaaactaat
    27361 acagcaacat agtaaagaga aactcctaac ttttctccta tataagtcaa taatatagcc
    27421 aatatagcat tacctatgaa accagtaata aaaattacat tatcatagtt atcttcaaaa
    27481 ttggatttta tcgctccaaa aatagaatcc attattgcaa gaatagctac tgacatatat
    27541 atggaataat ccatagaata tgtaaatgga atatagtaac ctactactac accaaatgct
    27601 aatcctccta aaacccataa cataaatctt cacctcttta ttttactttg aaacacaact
    27661 acattacaat cctttttatt aatttttttt actttttctt tatttttttg tatttttcgt
    27721 tttattttgc aaataaatcc actttatttc cattattttc ctgaactttt atattaattc
    27781 catatatatc cttaagagat ttagcatatg attctggaga gtttatagct gcactcaata
    27841 aatctatatc tcctatagct tttattataa atggttgtcc atatgtagta tcatttactg
    27901 taatagtagc tcctgaacat tttatcttac ttgttgctag tactctttct ccatttatag
    27961 aaattgctct tgctccagcc ttttttaaat catttaaaac cctcaaaata tctatatcat
    28021 gtataatcaa atcgtttgga ttctgtccgt cttttaactc tctctcacta tccctcatag
    28081 taatagttat gcctggccct gtaacagttg aataaccact taattcttta acttcttcaa
    28141 gctctttttg cataagtgtt tggatagatt catttccatt cttttttgct tctttatatt
    28201 cattcaaagt ttttttgttg tttttaagag tttcttttaa attttttatt tctttttttg
    28261 tattttctac ctcactttca atactttcag tttgacctaa tgttatgtat actctatttg
    28321 ggtctagtga ttttatataa gttgatatga aaacgcctaa tatgaatgct cctaatataa
    28381 ctattttctt tttcatacta ttatttcccc tctgctttgt ttatatcact atgaccattt
    28441 atttctatat tgtcttcttt ggtcactttt actttggcat tataatatgt ttctatttcc
    28501 cagacaatac catactttat attaagtgcc gaagccaatg tatcagaatc accaattgct
    28561 tttattataa ttggttcttt tatgacagtg tcattcatat aaagcttgtc ttcttttaaa
    28621 tacatatatg tattagaaac tattctctgg tcgtttatag atatggcact tgcttgtgct
    28681 gaatttaatt tatttatcat tgacaataat aaatcataat tatatagtat actatcattt
    28741 ttatcagagt ttcctaagtc ttttgtagaa tttatctcaa tttttatgcc tgttccttca
    28801 acatctgtat atccagctaa tgtttcatat ttttctattt ctgacttaag tatgtcttgt
    28861 tctccttcat tgtttttgcc tctataagtc tctatatctt tcttcacact attaacttct
    28921 gattgtagat tctctttttc tttttttaaa gatttaagtt cttctgtaag ttgctctcct
    28981 cttttaaaag ttgtcatccc tctattttct atattaataa ttttaagttg taaactaaca
    29041 agaacaccca atacaatact tcctatgatt atacttttat ttataaatct ttttttcaat
    29101 atatcacttc cttacaattg tctatccttc tggactatac actgccaact ttccatagtt
    29161 aagatttata gtccctccat tagtattttt attttgcagg tcaatcagaa ttttacttac
    29221 cctagatata ttataatcta agttatcatc attagaaagt aagattttta tattacttct
    29281 tgtaagcatt tctatattgc tttctttttt taagtttaca taatttattt ttctataaat
    29341 tccttcttct tttaaataat ttaaagtttt aaatacattc tccttagttt tataattttt
    29401 aaattttata gatttattat catcaatgct ataatctaca tctactatca agtctttttt
    29461 actttcatta gaacctctta gttcttccaa taaatttcct tttttatcta tataacaata
    29521 attatcttca tcttttaata ctgcaaatat ttctttttct tttaaactta taattatctt
    29581 attgggaagt tttcttttta tttccacatt ttctatatat ggatttttta taagtttatt
    29641 tttcatatct ttaaaattgt atgcaaaaat attttcattt agatttacat ttagttcttt
    29701 cattatattt gatttggtca ccctcttatt tccaattaca tctattttct ttacatcaaa
    29761 taaatcactt tttacaatta tacatataca aaaagataac ataagtataa aaactaaaac
    29821 tatcatgaca ttattcgtat ttaactttct tctttttttc aaatataatc aacccctttg
    29881 gttaataaat acacctttag agcttgtttt tcctacaaaa aaattataat atttaaagtc
    29941 aaatttttgc attttattac ataacatcca aataaggtat taaattttat aaataaatca
    30001 atatttcatc aaaataaaca tagcatacta catactattt atatatttaa atctgtatac
    30061 atacaaataa acccctaatt tataataatt tagaaaatct aatatattat aaattagggg
    30121 taaatattta cttatctttt ctttattcct attaccttag cttgaccttc tatacttgga
    30181 gtcgtttctt tttttatttc ttttacttcc ttaataactt tttctttttt tgttttttta
    30241 gaagttgact tttgtgttga attgtatact ttcattatct catcatatat aaggtctatg
    30301 gcttcaggct tacctatagt tttacttgca tttgccatat caaccaaaag ttctctatct
    30361 cctaaaagtt taaatactgc tgtatttagg ctctctggag ttagattttt ttctaaaata
    30421 gcaattcctg ccccttgttt ttctatactc ttggcattat actcttgatg attttcagca
    30481 gtataagctt taggtattat tatagatggc tttccaagag ccgttatctc agctaatgaa
    30541 attgctcctg cgctacctat aaccaaatca cttgctgcaa gtgcatttgc catatcttct
    30601 aagtaaggca caactttttg gtatggttta agatttatat cgctaatact tcccataaat
    30661 tcatcataat agcttttgcc tgtagcaaat ataaaagcta tatcttcatt taccatattc
    30721 tttattacaa gtctcattgc atcatttatt tttcttgaac ctccactacc accatagcaa
    30781 agtaccattc ttttttcgtc acttattgat agattctttc ttgcaatatt ttttcttgat
    30841 aataaaattt cttttctaac tggatttcct gtaaaaacta atttatcttc tgctgcctct
    30901 ggaaatcttt tatgcgaatc ttcaaaactt gtaagtacct ttgtaactgt tttagataaa
    30961 atcttattag ttacaccagg aaaagaattt tgctcatgta ttatggctgg tattttcccc
    31021 atagatgcat taaataaaac aggtccacta acatatccac cagttcctat aactatatct
    31081 ggtttaaatt tcttaacaat ctttcttgat tgctccaaac ctttaaaaag cttaaaaact
    31141 ctttttacat tatcgaaatc tatttttcgc ttaaaacctt gaactgttac agtctttaat
    31201 tcaaatccat acttaggaac tatttctgac tctattccct tttccgtccc aacaaaaatt
    31261 atctcagcgt ctggatgctc atctcttatt ttgtttgcaa tagctatagc tggatacacg
    31321 tgccctccag ttccaccgcc tgataataaa actttcatca aatcatcatc tcctgttaat
    31381 taattttgac atgctttgag atgtttaaca ctattcctac agctcccata aagatggtga
    31441 gtgaagttcc tccataactt ataaatggta gtgctacacc tgtagctggc attgatgatg
    31501 tcgcaactgc tatatttaaa gctgcttgta ttccaatttg tgcacctata cctataacaa
    31561 ccatacaagc aaatacatta gaagtcttca aagctattct tacacatctg tagactaaaa
    31621 ctacaaatag cataataact attatacaac caatcaatcc taactcttct cctattatag
    31681 caaatataaa gtcattttgt ggctctggta tataaaaata cttttgttta ctctttccta
    31741 agcctagacc aaacaatcca cctgaaccta gtgcatatag accttgtatt acctgatacc
    31801 ccttaccaag agggtcttga aaagggtcta aaaatgatgt aacacgacta agtctatagg
    31861 gttcagcaat tactaatgca gcaaataaag cagccccact ccctatcata gcaaatacaa
    31921 ttttcatatc cattccagca acaaatatca ttacaaaagt aactagaatt acagtacctg
    31981 ctgttgacaa atttggctga agtattatca acgcaaaaaa tatccctgga actacgagaa
    32041 gtggtacaac accttttgtc aaagatttta ttttatcata atttttttca attaattttg
    32101 cagttaatat tattgtagca aactttgcga tttctgcagg ttgaaatgtt aaagctccta
    32161 ttcccaacca tctttttgct ccgtttgctt ctatccctaa aggggtcaaa actaacaata
    32221 ataatactac tgctatagct ccaattgctg tagcattttt cttccaaaaa ctataatcta
    32281 ttcttgatgt aataatcata actataaatc caagtacagc atatattaca ttctttttta
    32341 gaaaatagta cgcatcattg tgtttaaatg aagattgtat aaaacttgca ctaaacacca
    32401 taactattcc tacaaatact aaaagcatag tagtgtaaaa cactactcca tcaaattcag
    32461 tcttcaccct tatgtcaatt tgttttttca aattttcttt aggcatccaa ctaatctacc
    32521 tccattcttc catttaagta agtttagagg taagagatta ttttaaattg tttacattat
    32581 ctttgaagtc ttttcctctt acttcaaaac tttcatacat atcccaactt gcacaagctg
    32641 gtgaaagaag cactatatct ccactttttg aaatttcata agaagtctta actgcttctt
    32701 ccatatcttt tactatatgt atgtcattaa atcctttatt tttagcacaa ttttctatat
    32761 ttgatgcagt ttctcctaat aagaccaatg atttaacata tgactttgca gtttcaagaa
    32821 gttcatcaaa attacttccc ttatccatcc cccctgctat aagtattata ggtctatcat
    32881 aagactgtat agcttttata gtggaatctg gattagttcc ctttgaatca tttacatata
    32941 ttacattgtc taaatttctt acaaactctt gtctgtgttc cactccttta aatgtcataa
    33001 gtacatcctt tataacttct aaatcaattt tgcagacata tgctattgct atagctgcca
    33061 taaaattttc taagttatga cctccaggta aacttacatc atctttattt agaaatttga
    33121 ttttatcatc tatatctatt attatgtcat tattttctaa atatactcct ccatttactt
    33181 ttttagttct tgaaaagtaa attactttgg cattgcattt atcagctaaa gattttacta
    33241 tctcatcatc atagtttaat acacagaaat cctcaacagt ttggttcata aatatatttg
    33301 cctttgcttc tatgtatttt tccatagtat ggtgtctatt taaatggtcc tctgtaatat
    33361 ttaatattgc gcttacttta ggtctaaatt catctatact ctcaagctga aaactgctta
    33421 gttcagttac taacacagac tcttcacttg aagtttctat agtatctatt acaggatttc
    33481 ctatattacc aactacatat gtatctcgtt ttgctcttga aaaaatttct cctaccaaac
    33541 tagttgtagt tgtctttcca tttgtacctg taatccctat aaaaattgga ttatccgata
    33601 gtttaaaagc taattctacc tcaccaatta tatacttgcc tgaatttttt aatttcaata
    33661 tgaatggtag gtctagtggt acacctggag acactacaac catatctata tcatctacat
    33721 cttctgggtg atgtcctaat atgtatttta tgtcatttat agatttcaat tcatcaagta
    33781 tatttctcaa tttattttca tcttttatat catttacgat tatacttgca cccaatttat
    33841 ctaaatgttt aattgtagat ataccagttt ttgcaagtcc tactaataaa actttttttc
    33901 cttttaagtc catttttcat tccccctagc cctaaaatac tgctattata cttatccaag
    33961 ctaaaacaac tgttataatc caaaaaataa atacaactct tgtttctggc caaccacact
    34021 gttcaaagtg gtggtgtatt ggagccatct taaagaatct ctttcttgtt gccttaaaat
    34081 atccaacttg aatcaaaact gatagagcct ccgcaaaata aattcctcca attataggta
    34141 ttataagcac tgagttagtt agaactgaaa atgctacaac tgcacctcct aatgccattg
    34201 aacctgtgtc gcccataaac actctagccg gatatgaatt aaatcctaag aaacctaaac
    34261 atgctccaac agttgctgct gctaaaactg cgacttctgt atttccagca atagaacttg
    34321 caaatagcat aaagaataca gaaactatta atgttactcc tgatgctaat ccatctaatc
    34381 catcagtcaa gtttacagca tttactattg ctacgattat aaacatcatt attggtatat
    34441 ataatattcc aacatttatc acaaaatctg taaatggtat cataagttgt gaagcacttg
    34501 atgatgatgt atactgataa aaagcaacat aaaaagataa tgcaacttga aggattattt
    34561 tttgatatgc ttttagtcca agtgatcttt ttagctttat ttttataaaa tcatctaaaa
    34621 agccaataaa accaaaacct gcaatacata taagacctac tgccatatca tgactaactt
    34681 ttactcttgt aagtccagtt attagaattg caacaatcat tataattcca cccatagtag
    34741 gtgttccgtt tttagctaga tgtgtttggg gtccatcatc tctaactgtc tgaccaaatt
    34801 taaattttct aagcattggt ataaatattg gtcctattat aattactatt aaaaacgcaa
    34861 tcaatgcggt gtacgtaagc tctgttattc ctaacatcat aaaactcctc tcccttgtca
    34921 attaactcat tagtttaagc ttatttattt agatattcga ctattttttc taatctcatg
    34981 cctcttgagc cttttactaa aatagtgtct cctgttttta ctaattcatt taacttgtta
    35041 aacacatctt ctctattctc aaagtgatat atattggctg aattgaatcc taattgtttt
    35101 gcttcttctc caataaacac tgaattttca cctatagtta ttataatatc agtattattc
    35161 atagatgatt ttccaactaa tctatgacca tactctgaaa tttcacccat ttctagtata
    35221 tctccaagta tcgctaccct tctaccttta tatcttccaa gtatctttaa agcagcatcc
    35281 atagaatctg gactagcatt atatgaatca ttaattattg ttaatttttc attttttatt
    35341 atgtctagtc tcatttttgt ccctttaaaa ttttttaaac ctttttttat ataatctaaa
    35401 gaaatattta gacacaaacc aactaatata gcagacattg cattatatat attatgttct
    35461 cctattgttg gtataaatat ttcctctttt ttcccatcta ttacacatat aaatgttaaa
    35521 ctatcctctt ccatagtata actttcacaa taaacatcat tgtttttatt aaagccaaat
    35581 gtttttaact tatatacatg ttctttttct tttaatgtag ataaaaactt atcatctcca
    35641 tttacaatta aagtatttgt ttcatcaaag tttgaagtaa tctccatctt tgctttcaaa
    35701 ataccctctt gtgaacctag tttttctaca tgtgataatc ctatattcga tatgactgca
    35761 attttaggat ttactatatc cactaaatat tttatttcat tgaatccaga catacccatc
    35821 tctattacag cacactcatg ttctttattt agattaaata aagtcaatgg aactccaaaa
    35881 tgattattta agtttttttc attttttagt atatttaact ttgcagatat ggcagagtaa
    35941 atcatatctc tagtagtagt ttttcccaca ctacctgtaa cacctataaa tggaatatca
    36001 aatttttctt tatagtattt tgaaatatct cctagagcca aagatgtatc ttttacctct
    36061 attaaattta tatccgaact ttctaatttt attccattac tttcattttt tataaaagtt
    36121 ttacatccac tatcatatgc agacttcata aatgtatgcc catctaaatt ttctccaact
    36181 atggctacaa atgcattttg ggcatttgcc tccctgctat ctataactat atcacttaca
    36241 cagtcattat aatctccata tattaacttc ccattagttg caagtactaa ctcttttatt
    36301 gttaagcact ccattattaa actcctcctt taaaacctta gctatattct cttagcaaca
    36361 aaaggcaatg tataccgtta tatatacatt gcctacgata actcttaact ttataattat
    36421 atactatatt tctgtttttt tgaagatgtt tatgggtata tgttaattat ctgttacttt
    36481 ttagttttca aaatataggg ttatacttga gtcttcatta acttttactc caggttttgg
    36541 gaatatatcc tttacctttg tatcttctgg aagttctata tctgcatcta agtctggttt
    36601 taacttcgca tcctccaatg cctttacagc atctcctatt tttagatttc ttacatctgg
    36661 cactttaact tgcttctttt catactcagc tttttcttct tctttatata ctggtttaac
    36721 tcccaaatat tttaaagagt cattcattat ttctttcact attggtcctg ctgtagtact
    36781 accaaatgca cttactcctg ttggttcgtc aacaatagca agaactacta tttgcgggtc
    36841 atcacaaggt gctattccaa caaatgaaca tatatacttt cctggagcat acttaccatc
    36901 tataactttt tgagctgtac ctgttttacc accaagtcta taacctggta tatacgctat
    36961 ctttcctcca ccttcagtta caactgattc tgcaatttct aacatttttt tagatgtttc
    37021 tttagatata acacttctta ctttttttgg ttttactgtt tcagttatat ttcctttatt
    37081 gtcagtataa gattttacca ctcttggttg cattaaatct ccaccattag ctattgatga
    37141 tatagctgtt ataagttgta ttggagttac tgatatagat tgaccaaatg atattgtagc
    37201 caattcaact ggtcctacat ttttttcgtt atacagtatt cccttagctt ctcctggtaa
    37261 atctatacct gtcttatcca taagaccaaa tgattctata taatcataca tttttccaac
    37321 acctaatctg cttcctaact caacaaatac tgggttgcaa gagttttgta ctgcttgttt
    37381 aaattcttgt gttccatgag gtctgtaatg tctccaacat ttaatctttc ttcctccaac
    37441 agttacacta cctgtacatg tgaatttttc tccatcttta attactcctt cttcaagagc
    37501 actggaagat gtaataagct taaatgtaga acctggttca tatgtatcac ttactgctgg
    37561 atttctccac atttgataat agccttttat tttatcttta tcattatatt tttcaagttc
    37621 ttcttgataa tatggatata ttggcgtcct tgagtcatta gggtcataat ctggttttga
    37681 tgctaaagca agtatatcac ctgttttagg gttcatagct attgcagtaa ccttttttgc
    37741 attatttaac tcataagctt tctgtactgc tttttcagta tagtgttgaa ttacttcatc
    37801 tatagacaac acaagaccat taccttgtac aggctcataa tatttttcca ttccttgtgg
    37861 tatttctctt cctgaagcat cagtacttac tataagtttt ccaggctttc cttttaactt
    37921 tttatcatat tgcatttcta caccagatat acctgttgca tctgaagaag tatgtcctaa
    37981 cacatatggt gcaaaattcc catatgggta atatctttga ttatcttctg ccacccatat
    38041 tccacttaat ttagcatctc ttatttgact tgccttatca tcatctatcc atctttttac
    38101 ttttacaaga gccatatttt ttttgcttat taaagcgtaa atatctttat aatcttcatc
    38161 caaaatctca gctacctttt cggctgcttc ttttttatct tctacttcca caggtttaca
    38221 ccaaacagta tacttagtta cacttactgc taactctttc atatttctat catatatagt
    38281 tcctcttttg ggttctattg gtatatctct tgtttgttgt tctagtgctt ttgtactcaa
    38341 ccaatttccc ttcataagtt gtagataccc agtcctaata acaaggcaaa aaaacaatgc
    38401 acatgctaga ataagaacga gtaccagcct tttcttactt atcctcttta cttttctcaa
    38461 atctattccc cctagttatc cacatctata tactttattt gtccatcttt tggatagtcc
    38521 atattgagca attccttagc ctttagctca atttctttta ttgatgtttt ttcagtttct
    38581 tttgccttta gttcctctaa tactatctct ttttttctta aatctttttg taaataatag
    38641 atatcgtact tcatttccga gatttttgaa taaccacata gattcaatat tattactgaa
    38701 aaagttgcaa tcagtaaaat gcatttagct cctatttttt tcttatttct ctttttataa
    38761 ttattctttt ttttattttt atattcattt agattatgta ttttataatt ttttttcaaa
    38821 ttctcacccc gatagtatat agtttaaatt ttcctctata ctatatttgt tattaatcat
    38881 atttcttcct ctaaaatttc atcgctaaat taatacacta ttacacaatc taattgtatt
    38941 tttttctata cacaacaaaa acctataaat tcacttatac ttttatatga atctataggt
    39001 tagatattaa tttctattta tttttatcac ttctagtatt tttagataat ttcttccata
    39061 aatataatat gtaactaact atctactctc tgattcctaa gttctccatt atattactta
    39121 ataccatcta tatctatatt tttatatcta aattttcatt atatatctca ataacaaaga
    39181 acatttctta tagtgttaat tgttttttaa ttgctttacc agtgcaacaa aatatataat
    39241 actttttaat taattataat aatcttatat ataagattat tctttagcct tataactact
    39301 tatatttttt ctgctactct tagcttagca cttcttgaac gtggatttac ttctatttct
    39361 tcctcactag gtaatatagg ttttctagtt attattttca cctcagattc tttatcacat
    39421 tggcatatag gtaaatgctg tggacatata caatctgatg ctaaatgctt aaatgcattt
    39481 ttcactattc tatcttctaa tgaatggaat gttattatac atattcttcc accttcattc
    39541 attatagaag aagcatcatt tatcatcttt gtaattactc caagttcatt atttacttct
    39601 attcgtattg cctgaaaagt tcttttagct gggtgaggtc catctattct ggctttctta
    39661 gggatagctt ttttaattac atctactaat tctcctgtag tttctattgg tttgtttgct
    39721 ctttcttcaa ctataaattt agctatacgc ttagcccagt tatcttctcc ataatctttt
    39781 attatcttag ttaattcatc ttctgtataa gtatttacaa catcatatgc agaaaactca
    39841 cacctaacat ccattctcat atcaagtggt gcatcttgca tataagaaaa tcctctatca
    39901 gcttcatcaa gttgatgaga tgaaactcct aaatcagcta atacaccatc aattttgtaa
    39961 actcctattt tttctaattc ctcttttata ttttcaaaat tgctgtgaac aaacttcact
    40021 ctactttcat attcactcaa tctctctttt gctgtgctta tagcattttt atcttggtca
    40081 aaccctataa ataatccctt atctgacagt ttttttacta tttctttaga atgtcctgct
    40141 cctcccatag tacagtcaac atacacgcca tctggcttta tatttaaatt ctctatacac
    40201 tcatttaaaa gaactgatac atgatgaaat tccatagtta cctcccttaa attcactttt
    40261 taaactattc ttcactaata ttatttttat ttttctttga taaataaaca tgtgcaatta
    40321 tgccaccaat aactccaatt agacttataa cttgtgccat tctaagtggt cctaacatta
    40381 aactatctgt tcttaatccc tctataaaaa atcttcctat tgaatataaa gttatataag
    40441 taactataac ttgaccttca tattttttat tttttctaaa tagtaataaa actataaata
    40501 ttccaaaatc ccatatagac tcatataaaa aagttggatg tacctttact ccatcaacca
    40561 ttattcccca aggtaaattg gtagcacctc catgtgcttc tccattaata aaatttcccc
    40621 atcttccaat agcttgtgct aaaggcattc caagtataac tgtatcagcc atttttaaga
    40681 agttaatatt tttaatctta gtgtatatat aaccagctaa tattccacca attaaccctc
    40741 catgtatagc aagtccacca cctctaaaat taaaaatttg agacatattt tgagcatagt
    40801 aactccaatt aaatacaaca taatagattc ttgcacagat tagtccaact ggaattgcta
    40861 ttatagctat atttaataca tcatcttctt taattcccac tctttttgct tcttttattg
    40921 ctattaaagt tcctaaaatc attccacatg ccataagtat tccataccac atgatgtcta
    40981 tgccaaacag tgtaaatgct actctatcca taacgtcacc tctttgtatt aattgaaaat
    41041 atttccaaag caatatgcta taattacaat taattgtaat tatactaaat tatgataaca
    41101 cattaatgtt aattctacaa aaaatattaa aatttattct acaactccct acactatatt
    41161 atatctaatt actataaaat tttttaaact aaatctctca tgactaaagt tacaagtgta
    41221 catagatatt ttttaatcaa aaagattgat gcatatatac atcaacctct tctaattctt
    41281 atctatagct cttaaatttt tacctcaatt tatataaata tattttataa atttgtttta
    41341 ccaaaataaa catttaaaaa cataaactaa tacttaatta gttattctta ttatgattaa
    41401 gtttctattt aggctctaca atagaaataa cacaatactc ttctttaaaa tgctctttta
    41461 atctttcttc aacttcctca aaagtaatat ctttaataat atctaaataa tccaatagat
    41521 ttatatcctt aaaaacataa gatataaaac tattacctat aaagtttata gaatcaaagc
    41581 acttaataaa tgaccctatt tttttctttt tagttctctc aaactcttct tttgataatc
    41641 cttctttttt tgatttttct atataatcaa gaattatttc ttttactttt ttaggctctt
    41701 tagaatctcc agctattatg ctaaaagcat aatccacttg agacgaaaat ccagctccaa
    41761 aattttcatt tattaaacct tgcatataca aatcttcata aagcttactt ccttttttaa
    41821 ataacatccc tacaagtata tcagttacaa tctctttacg taaaagttct ttccctttta
    41881 aaccaacatt gctatcttta aaacctatgt tgaacatagg catagatata gggaattttt
    41941 ctataacctc tttttctttt acactttctg gttcttctgg ataaaatctt tctatactct
    42001 tagagagtct gtctactttg taattgtttg actttttagt tacatctata actttttcta
    42061 catctaagtc accaacaaca aataaagcca tatttcctgg attatagaaa gtattataac
    42121 atttatatag ctcttcctta gttatcttat atatactatc aacagtacct gctatatcaa
    42181 ttctagcagg ataatttaca tacattgcct tcaagcaatt aaagtataca ttccaatcag
    42241 ggtcatcatt gtacattttt atctcttgtg ctattatacc tttttccttt tcaacatttt
    42301 catctgtaaa ataaggtgtt tgaacatagt caattagatg ttctaaactc tcataaaaat
    42361 tttcagtagc agaaaataag taggctgtca ttgtaaaatt agtaaatgca tttgcattta
    42421 ctcctaattt tgaaaattta tcaaatgcat ctcctccatc aggttgctca aacatcttat
    42481 gctctagaaa atgtgcaata ccttcattca ctctaatttt cttatcttct ccaattggta
    42541 caaattctaa gtcatttgaa ccataattag tagccaaaat agcatacttt ttcataaaac
    42601 cccttttagg cataaaatat acatctaatc cattttgcaa tttctcataa taaacttctt
    42661 ctttcaatat atcattaact attttttcca tagacatctc ctccattaat ttctcaagaa
    42721 gtatacagta tcaagctcta tattttttat agcttcaact atatcttttt ttgtaacctt
    42781 ttctatacta gatattatgt cttgtacttc agaatttgtc tttgccatag actgagagaa
    42841 aacaaaatca gacataccac ctatattatc agttatagat ttcatagaat taactaaagc
    42901 aagtttactg ttttctaatt cttcatcaga tatatcacct gcttttacac tttctacttg
    42961 tttttttatc aattctacag ctttatcata atctttagtt tctataccag atgatataaa
    43021 catgatactt ttatacttct ctattgaaga gaatatatag taacataaac tttctttttc
    43081 tcttacatta acaaataatt tagagtgtgg accaccacct aagacattgc tcccaactac
    43141 taaagcataa tatttatcta tgtctgcata atccacattt gctctataac ccataactaa
    43201 cttaccttga gttatttcca tttgttcatc tataactttt acctcatcca cattttttat
    43261 aaaatctgct cttggtatat ttataatttc ttctctttca aattttagat ttttacttat
    43321 tatatctaca actttatctt catcaaaatt accttctaca actatatcta taggtgatgt
    43381 tttaagtatg tttttataat gttcatataa ctctcttgaa gttatactat ctatttcatc
    43441 ttcataacca aattcactta cactatatct ctctccttta cacatagcct caatacattt
    43501 atcttgcgca tattcctttt tatcatttat tattgattgt atcttttctc ttaaattctc
    43561 tttttcaata tttaaatatt cttctttaaa cccaccatct acaacaagcg tattatttat
    43621 tacttcatta aaaaattcta ctacttcttc aaatatactc tcatctaaat acttttcact
    43681 tatgttaatt actttaaaac tcaatacttg acgttctcca cgttttacag catcagctcc
    43741 catagatgaa ccatataaat catctaattt attggatatt gctctagcac taggatattt
    43801 agcgcttcca cttgctatta ttgatggtat tagagcattt tttgtagttt ctttttcatc
    43861 caatagtctc tgaacatata aacttataag attcgattta aacttttctg tttttatcaa
    43921 agtaagatta atattattac ctaaatttat tattttttta gtattaacca tatgtacctc
    43981 ctatttttat ttaaacatca attttaaaat tgaaatttat ttatttttta attacatata
    44041 ctcacatata aaaaccttct atttaattat atccttttta tta
//
LOCUS       DAMJYX010000022        41572 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJYX010000022 DAMJYX010000000
VERSION     DAMJYX010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.022"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            149..2242
                     /locus_tag="PC479_001332"
     CDS             149..2242
                     /locus_tag="PC479_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2803885.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            complement(3295..3579)
                     /locus_tag="PC479_001333"
     CDS             complement(3295..3579)
                     /locus_tag="PC479_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803886.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            3786..4604
                     /locus_tag="PC479_001334"
     CDS             3786..4604
                     /locus_tag="PC479_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2803887.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(4737..6857)
                     /gene="feoB"
                     /locus_tag="PC479_001335"
     CDS             complement(4737..6857)
                     /gene="feoB"
                     /locus_tag="PC479_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2803888.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(6883..7110)
                     /locus_tag="PC479_001336"
     CDS             complement(6883..7110)
                     /locus_tag="PC479_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2803889.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(7437..7877)
                     /locus_tag="PC479_001337"
     CDS             complement(7437..7877)
                     /locus_tag="PC479_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2803890.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            8029..9018
                     /locus_tag="PC479_001338"
     CDS             8029..9018
                     /locus_tag="PC479_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2803891.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    9170..9422
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9495..10703
                     /locus_tag="PC479_001339"
     CDS             9495..10703
                     /locus_tag="PC479_001339"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2803892.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(10764..11645)
                     /locus_tag="PC479_001340"
     CDS             complement(10764..11645)
                     /locus_tag="PC479_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2803893.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            12031..12975
                     /locus_tag="PC479_001341"
     CDS             12031..12975
                     /locus_tag="PC479_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2803894.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            13198..13743
                     /locus_tag="PC479_001342"
     CDS             13198..13743
                     /locus_tag="PC479_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2803895.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            13834..14982
                     /locus_tag="PC479_001343"
     CDS             13834..14982
                     /locus_tag="PC479_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2803896.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            15035..16300
                     /locus_tag="PC479_001344"
     CDS             15035..16300
                     /locus_tag="PC479_001344"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2803897.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(16586..17818)
                     /locus_tag="PC479_001345"
     CDS             complement(16586..17818)
                     /locus_tag="PC479_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803898.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(17821..18495)
                     /locus_tag="PC479_001346"
     CDS             complement(17821..18495)
                     /locus_tag="PC479_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803899.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(18503..19732)
                     /locus_tag="PC479_001347"
     CDS             complement(18503..19732)
                     /locus_tag="PC479_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2803900.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(19844..20848)
                     /locus_tag="PC479_001348"
     CDS             complement(19844..20848)
                     /locus_tag="PC479_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2803901.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            complement(20872..21546)
                     /locus_tag="PC479_001349"
     CDS             complement(20872..21546)
                     /locus_tag="PC479_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2803902.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            21834..22601
                     /locus_tag="PC479_001350"
     CDS             21834..22601
                     /locus_tag="PC479_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803903.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            22588..24525
                     /locus_tag="PC479_001351"
     CDS             22588..24525
                     /locus_tag="PC479_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803904.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            24750..26363
                     /gene="ggt"
                     /locus_tag="PC479_001352"
     CDS             24750..26363
                     /gene="ggt"
                     /locus_tag="PC479_001352"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2803905.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(26496..27347)
                     /locus_tag="PC479_001353"
     CDS             complement(26496..27347)
                     /locus_tag="PC479_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2803906.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            27973..28866
                     /locus_tag="PC479_001354"
     CDS             27973..28866
                     /locus_tag="PC479_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2803907.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            28866..30260
                     /gene="gltA"
                     /locus_tag="PC479_001355"
     CDS             28866..30260
                     /gene="gltA"
                     /locus_tag="PC479_001355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2803908.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            30626..32845
                     /locus_tag="PC479_001356"
     CDS             30626..32845
                     /locus_tag="PC479_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2803909.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            32992..33738
                     /locus_tag="PC479_001357"
     CDS             32992..33738
                     /locus_tag="PC479_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803910.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            33738..35618
                     /locus_tag="PC479_001358"
     CDS             33738..35618
                     /locus_tag="PC479_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2803911.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            35737..37128
                     /locus_tag="PC479_001359"
     CDS             35737..37128
                     /locus_tag="PC479_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2803912.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPETIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            37235..38275
                     /locus_tag="PC479_001360"
     CDS             37235..38275
                     /locus_tag="PC479_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2803913.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            38764..39417
                     /locus_tag="PC479_001361"
     CDS             38764..39417
                     /locus_tag="PC479_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2803914.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            39493..39702
                     /locus_tag="PC479_001362"
     CDS             39493..39702
                     /locus_tag="PC479_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803915.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(39842..40408)
                     /locus_tag="PC479_001363"
     CDS             complement(39842..40408)
                     /locus_tag="PC479_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2803916.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            40569..41222
                     /locus_tag="PC479_001364"
     CDS             40569..41222
                     /locus_tag="PC479_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2803917.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(41380..41556)
                     /locus_tag="PC479_001365"
     CDS             complement(41380..41556)
                     /locus_tag="PC479_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803918.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT        15784 a         4764 c         6557 g        14467 t
ORIGIN      
        1 tcaattataa tttaaaatat gaagtaataa actttctaat taaattaagg tatttagtgt
       61 atatgtataa gttataagta aatcttctaa aaagaataat tttaacaatg ttgttataaa
      121 gagcagtaac tatggaggag ataagtatat gaacaaacat aattttgaag ttatattaaa
      181 tcaactacag attaatatat atgttacaaa tattcatact aatgaaatta tttttatgaa
      241 caaaaaaatg aaagaagaat ataatatttt agatccagaa ggaaaagttt gttggcaagt
      301 attatatcct gaaaaaaatt ctacttgtag tttttgtaaa gttttagaat tattaaagaa
      361 tgataaaaaa ggagtattaa taaagtggta tgaaaaatgt aataagttaa atcgtgtttt
      421 tgaaaattat gatagtttaa ttacatggca agatggcaca gttgttcata tgcatcaatc
      481 aatagatatt gcaaactcaa caagcttaaa taaaccaatt aagattaatg aatttcatga
      541 aatatcaaat aataaagaag aaaaaggagt ttttaatttt tcaagagaca attttgatta
      601 taattctaca ttgctttacg atgctttgat tagaggtaca gatgaatata tatacatttg
      661 caatatgaaa acaggcgtat ttcgatattc accatcacaa gttgaattat ttgacttacc
      721 aggagaaatt gttaaaaatc cgcttgttta ctggaaaaaa attgtacatc cagaagactg
      781 gaatcgtttt tataagtcaa acactgaaat aggtaaaaac cagatggatt atcatacagt
      841 ggaatttagg gctaaaaatc gtagtggaga atatatatgg ttaagatgta gaggtcaact
      901 aatgcgtgat gaatttggag agccaagtat ttttgcagga ataatgacgc aattaggtaa
      961 acaaaataag attgattcat tgacacagtt actgaattat catgaattta tgagtgtatt
     1021 tgaagataaa atttcaaatc ctatgattga aaaattatgc atagtattat tagatattga
     1081 cgattttaaa aatgttaatg agatgtatga tagggatttt ggagataata taataaaaac
     1141 actagcacag tctgtacaat ctattttacc agataatgct gaactttata aacttgatgg
     1201 ggacgaaatg ggcattcttg ttgataatgt tgaagaaaat gaaattctaa ctctatataa
     1261 tcaaatacag aatatgatta tacaccttca actatggaga aaatatggat taaatataac
     1321 aatttctgct ggttgtgtaa tatatccaaa acatggagat acagtaaaag aactttataa
     1381 gtgtgctagt tactctttac aatatgcgaa agagcatggt aaaaacaggc ttgttttttt
     1441 ctcccaagaa atattaaaaa ataaaatgta ttcattagaa atgatgcggg atttaaaggc
     1501 atcaattaat gatgatttta gggggtttag tcttcgattc cagccacaag tagatacaga
     1561 aagtcataaa ataataggtg tagaagttct tttaaggtgg actaatgaca aatgcaaagc
     1621 aatttctcct ttggaattta ttccaatatt agaagaaaat gatatgataa atattgtagg
     1681 ggcttgggtg cttcgaatgg cattacgtac ttttaggaaa tggattgatt actatccttt
     1741 ttttaaagtg agtgtaaacg tttcagctgt tcaaatatta gaagacacat ttattgaaga
     1801 tatagtaaaa attatagatg atgaaaattt tccttatcaa aatcttgttt tagaattgac
     1861 agaaagtcat acagtacaaa atatgagtat tttacagttt aaatttaagg ctttacaaga
     1921 tttaggaatt tatattgcta tggatgattt tggtactgga tactcgtctc tagaagtttt
     1981 aaaattttct ccaatagata tagtgaaaat tgatagagta tttgttaaag atatattaaa
     2041 gagtaagttt gatgctacat ttatacattt tattgttgca atttgtcatg atgtaggaat
     2101 aaaagtatgt ttagaaggtg tagaaacaca agaagagtat gatttagtta aacaaataaa
     2161 gccagattac atacaaggat atttatttgg aaaaccgcag acagcaacag aaatctttga
     2221 ccttttaaag ctagataatt aattctaatt tatcaataat atcactgaaa agctttaaaa
     2281 tcaaaaatac attaaattat tatattgaag atatatcaat tataaattgc aatataagaa
     2341 aatagcattt caatggatgt taaattggga atagtatatg attaaagtat tttaaaaagg
     2401 gataaccata ataaaaatag atatctattt ttagattaaa aataatatat cataaataaa
     2461 ataataagag gtagatacag ttttaaggga atacaaaagt ttttaattaa actatgcttg
     2521 ttcagataga tatttattta agaaaaaaga ctattaaaag caatatacaa gaatgatata
     2581 ttagattgat taaacaagca taaatattat gtaaaaaact ttaagttata gaatttaaat
     2641 ctaatgtaga tagattacgt ttttttgctt ttattatggt ataaattggt atcaatattc
     2701 aaaagtaata tatttatgat ataataaaat tataggaatt ttgcagtgag cgatatttgt
     2761 gaaaaaattt ggcgtaacag ttgaaatata aggcgttgag agtgcatgat aagcgttatc
     2821 aattgcacta ttgctcgttc actgcaaatt taggagagtt gtatacgtgt aagtgttgaa
     2881 aatactaagt ttattttggt gttttatatt aactatatgg aatgtaaatg catgttaaag
     2941 tggctaattg gaactatgat tacaacgttt tatattaact atatggaatg taaatatttg
     3001 attttaatgg tacgttttcg ctttctctaa ggttttatat taactatatg gaatgtaaat
     3061 ctatcttttg ttgctttaca taaatttata ttacttatgt tttatattaa ctatacagaa
     3121 tgtaagtttt cataattttc ttttaatgtg ttatcttatg attatctagt tacattagca
     3181 tacaaaaata taataaaatt acactatatt ataaaaacaa aaaggtagta tagaaatcct
     3241 attacctttt tattattaat tttatcagtg ttttattata aatagcttac ataattacac
     3301 attttttctg attaaataat atgatgctat tgttataata gatactaatg ctaatgatgt
     3361 tattgcagta tctaatcttc ttattaataa actcatttct atatattcaa gttctattaa
     3421 atactgataa aatatttcta gtttaacagc tttttctcta tctatatttc cgtactttaa
     3481 ctctaattta tctaaagatt ttttattaca ataattaact ttatgtttaa ttaaatatct
     3541 tattgtagca cctattacaa ttttcactct aacaagtata aatataatat tccatccaaa
     3601 agttaagagg ggatatcttt tttatgatgg gaaagggtaa aatatttata actgacatct
     3661 aactgatatt taagctttaa aatcaaaacc atattgattt tctaaaataa taactacttg
     3721 atttagtaaa taataaagaa tatagtaact atatagacaa caaaaagaaa aggagtgtat
     3781 aacccttgga aaaacagtat tttacaacag gtgaatttgc taaattgtgt ggtatatcta
     3841 agcagacatt aattttctat gacaaaatag gtattttttc tcctgaatat aaagataaaa
     3901 ataattatag atattactca gtttatcaat atgatacact tgatatatta caatcattaa
     3961 gagaaatagg aatgagttta gaggaaataa aagagtatat acaaaataga actccccagc
     4021 tttgtgtgaa aatgcttaaa gaagaagaaa aaaagataag agaaaaaata aaaaaactaa
     4081 gaaagataag ttctaagatg caaaatagaa taaatataac agttgaagga atttctaaaa
     4141 tgaatagtga agaaatttat atatccaaaa agtttgaaga atatttagtt ctatcaagcg
     4201 atttgagtaa tatgagtaat agtgatttta tgatggaatt aatagatttt accaattatt
     4261 gtaaatcgaa aaatttgtat caaggatatg agttgggagt aatagtaagt aatgaaaata
     4321 taaaaaatgg ggactattta agtatatcaa gtttttattt gaagatagat aaaaaaataa
     4381 aggataaaaa gctatatgta aagccagaag gaatgtatgc ttgcataaaa catattggaa
     4441 aatatgagga ttcatataaa tcttacgaaa aattgaaaaa atatatatat gaaaatgaat
     4501 ataaaattgt tggaaattcg tatgaggaga gtatacttga ctttttttgt gaaagtaatg
     4561 aagataatta tatgactgaa atttcaatcc aagtatccag ataagaatta aaatagagat
     4621 atattcaaat gttaagtaat tttacttatc attgaatata tctctatgct ttataataaa
     4681 gtatataaat ttttaaatgg tttctctaat atatgtctac tgtcagttaa tactaattat
     4741 ataaataaat tgtaaactgt agttgttaaa aaacaaacaa caaaagcaag tatagtaggc
     4801 attaagaatc ctaaggcagt ccattttaaa ctaccagttt ctttttttat tgttaaaagt
     4861 gttgttgcac aaggccaatg cagtaaactg aacagcataa catttaaagc tgtcaaataa
     4921 gtccatccat gttctctaag aacttgacct aaagcactaa agctatctaa ttctatcata
     4981 gaaccagttg ctaggtaagc cattaaaagt ataggtacaa ctatctcatt tgctgggaat
     5041 ccaagaataa aagcaagtaa tataaaacca tcaagaccaa ttaatttagc taaagggtct
     5101 aaaaagtttg caacatgtga taatatactt aaatctccta tatatatatt tgcaaatatc
     5161 caagttatga caccagcagg tatggctacc attacagccc ttcctaaaac aaatattgtt
     5221 ctatctatta tagaggtata taaagttctt cctatttgag gaactctata aggaggaagt
     5281 tctaatgtaa atgtagacgg aactcctttt aatagcgtct tagaaagagt gtaagaaacc
     5341 aacaatgtta ttataacacc tagaattatc agtaaagtta tgcaaagtgc agttgctacg
     5401 cttgatacga aagagttggt tataactgag ctaaaaaata ttgtagatat agctatcaaa
     5461 gtaggaaaac gaccattaca aggaacaaag ttgtttgtaa gtatagctat aagtctttcc
     5521 cttggagagt caattatcct acaaccaata actccagctg cattacatcc aaatcccata
     5581 cacatagtca aacactgttt tccatgtgca caagcttttt taaataggtg gtctaagtta
     5641 aatgcaactc ttggcaaata tccaaaatcc tcaagtaggg taaataatgg aaaaaatatt
     5701 gccataggag gtagcattac agagattacc catgctaatg ttctgtataa tcctaaaacc
     5761 aacatatcat ttaaccatga tggacaatta atactattta atatacccga aatacttggc
     5821 tcaaatccga gtaataaatt agataataaa gtggatggat agttagctcc ttctatagta
     5881 atccacaata tagtacctaa taataagagc attattggaa gcccaaatat tttagatgtt
     5941 aatattttat ctactttttc ttctctatca gttgattttt tagcaacatt agaacaacac
     6001 tcatcactca gttttttagc ataatcatag ttgattttag taaactcatc tcttattttc
     6061 tgtttattta tattgtctgg aatctttttc tttacttcat taatagcatc tatagaattt
     6121 ttgtctatat aattactcat agattcaaat atactttcat cgccatcaat aagtctaagt
     6181 cccaaccatc tagaatttat acctggtata atattatcca attctggttg aattgatttt
     6241 accacattct ctatattttc attgtatcta acaggtttat tatttaattt atatttgtca
     6301 aaagatacat catttaaagt atccaatagt tcatccatac cagagccatt tcttgcagca
     6361 gttaaaataa ctggaatacc aagagaatcc tctaaaagtt ttttatcaat agtaatgcct
     6421 ttttttctag cttcatctat taaattaata cacaaaataa ctttatctgt aagttccatc
     6481 acttgaaata ctaaatttaa atttctttca agacatgtag catcacatac tactattact
     6541 gcatctggat taccaaaaca tataaaatca cgagctacaa tttcttcttg tgataaggca
     6601 aataaagaat atgttcctgg taaatctatt aatgcatatt cggtattttt atatttaaaa
     6661 ttaccacgag ctgtagctac agtttttcct ggccagtttc cagtatgctg tctaagtcct
     6721 gtaagatgat taaatacagt acttttacct gtatttggat tgccagccaa tgcaattaca
     6781 aactggtctt ccttattgtc tatatcaaac atgtctttta aagaactcat tttagttgag
     6841 ttatgtgtca accccatatt aatatcctcc tgctagaaat ttttaaatgt cagaaactaa
     6901 tattaagcta ctttcttctt gtcgtagagc tatcttgcta cctctgattt tatagacagt
     6961 taaattattt tttggacctt ttctaactac atctataaca gctcctctag taagaccaag
     7021 cgctaacatt ctttctctta aattaccact agataatata tcttcaactt tgacagtttt
     7081 tttcacttga atatcgttaa ggtttttcat aatagcacct ccatgcttga aatttagcct
     7141 gggctaatct tcttatttta aactatgcat tatattatta ccttgttact tttacaaaaa
     7201 aaagataggc aagatgccta tcatacaaag ttgggtataa ttcttacttt tggggaaagt
     7261 agaatataga ataaaaatta acactttgaa tccatataat tattgaattt tgataataaa
     7321 aaagtcagaa agcaaaagcc ttctgactct atgtaaggta ttttcaaata cctaaatatc
     7381 aattcaatat ttaatttata cttaaattat atattaacaa tattagcttg tcaacactat
     7441 aatctcattt taatatgttc aatacctgct tcgtcgtata cttctgagat ttctttaaaa
     7501 ccacaagata agtaaaggtt ctttatataa gtttgtgctg ataaggttat attattttcg
     7561 tgtaagttaa cttttataaa atctatagca caatccatca tttgttttgc taatcctttt
     7621 cttctatgac tagaaagaac taaaactcta ccaatggaaa catcattata agagctgtat
     7681 tctttaggaa ttattctagc gtaagcacaa atcaatccat tttcttttaa aaatatgtga
     7741 taagagtttt tatctatatc atcataatcg tttaatgaaa atatcttctg ttcacaagca
     7801 aatacctcat atcttgattt tgctatttca taaaattcat ctaaactcaa atcattaaag
     7861 tgtttaattt taaacatagt aattacctcc tagcatctta aaaaactgaa tattaagata
     7921 cattatacca tttttaacat aaataccttt attaaaattt atatttatta aaaatatttt
     7981 taaaaagtga tatactatat taaaaacaaa actttagtta gaaaatttat gatttatgga
     8041 ggcagtgttt tgaatactac taaaaaatta actgaagcag ctttattgtc atctttattc
     8101 atagtagtaa caattatagc tgtgagtact ggatttggat atgctattta cttagatttt
     8161 atagtgccag tatttttttg tataatctgc ttgaaatgtg atttaaagta taccatttta
     8221 tctggaatta cttcgctttt aataatttct ctggtacttg gaaatctagg aactgctatt
     8281 tgggcaagtc agagtgtatt acttggtata atgtgtggct atctaataaa taagcctaca
     8341 atggttatgg atgatttagt atatggttca gtatttgggc ttattgtaat ggtttttatt
     8401 gatatatatg cttcaacatt aataggttat agttttatga aagaatttca aggatatagc
     8461 aaatggattt attttaatgg gtatacaaat tttatttatt atttaatgat agcattgttt
     8521 ccttttggta tggtgttttg catatattta ttaagtttaa ttttagggaa taaattgcat
     8581 attttagatt ctaattctct aaaaaaattt ttaatattta agaattttag aagtttaaac
     8641 caatttttat gttgctcaaa aaaagctttt tacatatgtg tttcttatct agccattttt
     8701 gaagttctaa aactgttgaa tgttaaagtt gattctgttt atttaaaaac aatctttata
     8761 tcaataacct atataagtct ctattttatc attagagact catgtatgtc actacaaaac
     8821 tttataatag ctaagtttag aaaattatta tatgctagaa ttttattttt gataataatt
     8881 ttattgctgt tttttatatt tgatgtaaca gttattggtt taataattat aaacgcattt
     8941 ttaaataaga aaataaatat aagattgagt cagagtaata tagtcaataa acaaataaat
     9001 cttttgattg aaaaataaat tttgggtatt ggtaaaaaat ataaaaaagt agtataatgt
     9061 aaaaaaacat gtatataata ataaaaaagg ttgacaaaat ataaaaataa taatatatta
     9121 tgagtaagtt aagaaaaatt tcttgtaatt gatgaaataa tctgaatata taaatgctat
     9181 gaagagaaga agtagggcta gattttattt aacagagagt tccagtttgg tgaaatggaa
     9241 taaattgatt tagtttgaat acatctcaga gcactatctc tgaaagataa caaccattaa
     9301 gagatatcgg attagtacac gttatagtac acgagtatat gtaattgtac tctaagagat
     9361 taatatcgtg agatattgat gaactaaggt ggtaacacgt aagcaatgct ttcgtccttt
     9421 taagaggatg aaagcttttt ttattataaa aataaaaatt atagattata taaatatttt
     9481 aggaggaatg aaaaatgaaa tcgatagacg agcaaatgcg aataattatg aaaggtgtag
     9541 atgatttaat agatgagaaa gagttaaggg aaaagcttat taaatcagaa aaagaaggaa
     9601 agcctatgat agtaaaattg ggtctagacc caagtgctcc agatatacat ttagggcata
     9661 cagtagttct tagaaagatg aaacaattac aagatttagg tcatcaaata gtaataataa
     9721 ttggagattt tacaggaaaa attggagacc caactggaaa atctaaagct agaaaagctt
     9781 taacaacaga gcaagttctt gcaaatgcaa aaacttatga agaacaaatt ttcaaagttt
     9841 tagataaaga aaaaactatt gtaagattta atagcgagtg gttagctaag ttaaattttg
     9901 aagatgtaat aaaattagca gcaactatta ctgttgcgag aatgttagaa agagaagatt
     9961 ttaaaaagag atatgaagga caaatgccaa tatcagtaca tgagttcttc tacccattaa
    10021 tgcaagcata tgactctata gcattagaag ctgatataga acttggtgga actgaccaaa
    10081 gattcaattt actaatggga agatcattac aaagagaatt tggtatggag tctcaaatag
    10141 taataatgat gcctttaata gaaggattag atggtaaaga aaaaatgagt aagagtttag
    10201 gtaactatat tggaatagat gaagaagcgg gaataatgta ccaaaaatct atggaaattc
    10261 ctgatgaact tataattaaa tactacaatt tagtgacaga tgttcatcct gatgaagtta
    10321 ataaaataga atcacagttg aaagagggtt cagtaaatcc aagagatata aagatgaact
    10381 tagcaagaga aatagtaaca ttataccatg gagaagaatc tgctaaagaa gctgaagaaa
    10441 gatttaaatc tgtattccaa aaaggtcaaa tacctgaaga tatacaaaca attcaagtta
    10501 aggaagatgg atttgactta atcgaagtac tagtttctaa tgagattgtt aagagtaaga
    10561 gtgaagttag aagattagct tctcaaggtg gagtaaaggt aaatggtgaa aaagtagagg
    10621 atttaagcac tattgtaaaa gagagtgaat tagttgttca aataggaaag aaaaaatttg
    10681 taaaaatcga gctagtaaaa tagagtaaag taaaagcaag tctattgagt ataaaatgta
    10741 cattcaataa acttgctttt ttattatatt aaaattttaa attgataacc attgtcttta
    10801 aaatatgtta taatttggtc caatgcattt actgtttctt gttttccata agtatcatgc
    10861 attaaaagaa caaccatttc tttgccttca gaagatttct ttgcatgttc aaataattct
    10921 tgtgcatttt tctttttacc ttctgcatca gcatttaaag cgttccaatc aattgatgcc
    10981 atgtttttct cttttaagta gtttcctaga ggttccatat ttttccatga catatacccg
    11041 cctgggcatc taactacatt tgaggaaaaa ttctttccta agactttttt catagcttca
    11101 tcagttttgt taagttcatt tacgaatgta tcgagattta ggcttctgtt aggatatagt
    11161 tttttatagt catgagtata agagtgatgg gctattgcat ttccttcgtc aaatgttctt
    11221 tttaaaattt cattagctcc ttttctttca agagaatcac ccttaataaa gaaagttcct
    11281 ctaacaccat gtcttttcaa gatatctaga acttgtggag tattagtagt agatggtcca
    11341 tcatcaaatg taagaaagac tatcttttct ccattgtttg aatagtcgta tttgttaagt
    11401 ttgtcccaaa ctatttttgc gtcatatcca tattctttta cagcttcttt ttttgcacca
    11461 ataataggtt catttttttc tttttcaatt ctttcttttt ctaatctttc agctttaagt
    11521 ctttcttgag tttctttcat ttcttttttt ctagacatgt ccgcccatgc catagaaaaa
    11581 ataccaataa ctattacaac aagtatagta agtgtaacag ttttcttttt tctttttttt
    11641 accataatta cagcattccc ccttttgtcg aattgtatca aaaatatatt ataattatat
    11701 cgaagaaagt agaaaaagta aaccgacaaa gttgtaacta agataactaa agtgtaactt
    11761 atatgacatt atttgcataa tattaaagta tcatatatat taaaaatatc tataaaaaaa
    11821 tttagtatag gtaaaaaaat ataaaaaata taaaaattta ttaaaaaggt gtttacaaaa
    11881 aatgattaat atattactgt taaatagtgg atataaattt gagctcaaaa tgtatgtaaa
    11941 tttagattta aaatactgaa aaaatttact atattaattt atagtaatgc ttatagaaaa
    12001 actctcttgc tgtttagggg gtttataatt ttgcaaaaaa ataaaaagga gggggcttta
    12061 ttgaaaaaat actttggaga aataggacta atatttatag caattatatg gggaagtggc
    12121 tttgtagcaa cccaatttgc attagacggt gggcttacac ctctacaaat cataacttta
    12181 agatttttct tggcagctat aattatgaat ttactgtttt ttaaacagat tcgtgctaat
    12241 atgggtaaaa aattattaaa agcaggaggg attttaggaa tatttttatt tctagcattc
    12301 acagtacaaa ctattggact tatgtacact acaccatcta aaaatgcgtt tataacagca
    12361 gctaatgttg taatagttcc attcatagga tttatacttt atagaagaaa acttgataaa
    12421 ataggaataa taagtagttt ggtagctctt ataggaattg ggatactttc tttagaagct
    12481 gatttctcta ttaattttgg agattttcta acacttatat gttcattcgg atttgcattt
    12541 catatattct ttacaagtga gtttgcaaaa gataacaacc caatggcatt gacagctatt
    12601 caatttacag tagcgttttt aatgtctgtt gtagttcaaa catttgcagg tcaattaaag
    12661 atggaagctg aattatctgg ttacatggga actatgtatc tagctgtatt tagtactaca
    12721 attggatttt tatttcagac tatatgtcaa aaaagagttg atggaactag aacagctata
    12781 atattatcca cagaggctgt atttggtaca atattttcta taataatttt aaaggagctt
    12841 attacagcaa aattggttat tggtagtatc ttgatatttg ttgcaatcat aactgcagaa
    12901 actaaattat cttttttaaa gagcaaaaaa gtgaaactta aagattcaga ggagtcttca
    12961 ttagaaagta tttaatatga caactatttc ctaattaatc aaataaatta tgtattatgt
    13021 agaaatttga catcaaaact tggttgatac tggtgtttca aagtgataaa attaattagg
    13081 aaataaattt tatttttagt gtttaagcta taaagtctgg ggtatatata ttcttgtagc
    13141 aagttattaa acttaaaaat aaaaatttaa ttatataaaa ttgggaggaa attaattatg
    13201 aaaaaatttg tttgtacagt atgtggatat atacatgaag gagatgctgc accagcacaa
    13261 tgtccagtat gtaaagttgg agctgataaa tttgaagaaa tgaaaggcga aatggtttgg
    13321 gctgatgaac atagaatagg agtagctcaa ggtgtagatg cagaaataat cgaaggatta
    13381 agagccaact ttactggtga gtgtacagaa gtaggaatgt atttagcaat gagtagacaa
    13441 gctgatagag aaggttatcc agaagtagct gaagcgtata agagaatagc ttttgaagag
    13501 gctgaacatg ctgctaaatt tgcagaactt cttggagaag ttgtagttgc agatacaaaa
    13561 gaaaacttaa gagttagagt tgatgctgag tatggtgcaa ctgatggaaa attaaaatta
    13621 gctaagagag ctaaagaatt aggattagat gctatacatg atacagtaca tgaaatgtgt
    13681 aaagacgaag ctagacatgg taaagcattc ttaggattat taaacagaca ttttggaaaa
    13741 taataattaa aacatagatt aaaaggagaa actttggttt ctcctttttt aaattataca
    13801 aataaactgt tgtgatagaa ggatggagat aatatgaagt cggtatctgt taggagttgt
    13861 aaaacttaca attatgaaga agtaaagtta tctattgaaa aaaatttaga ggatattggt
    13921 ggtatagata agtttgttaa aaaaggaagc aaagtactta taaaaccaaa tttattaatg
    13981 aaaaaaaagc ctgaagaagc tacaactaca catcctatgg ttgtaaaagt tgtatgtgaa
    14041 aaacttttag agttaaattg tgatattaca atagcagata gccctggtgg tccatataat
    14101 caaagttctc ttaagagcat ttataaggca tctggaattg aagaagtagc aaatgaactg
    14161 ggcattaagt taaattatga tgtttcagat gttaagatac atagtgaaaa agcacatgtt
    14221 ctaaaatata tggatattat taaaccaata gtagattcag attatataat aaatctttgt
    14281 aaattaaaga ctcatataat ggcaactttt actggtggta caaaaaactt atatggatgc
    14341 atagctggat tgaaaaaagc agagatacat tatagatttc ctacagaaga gttgttttgc
    14401 gaaaatgtac ttttagatat ttgtgattat ataaaaccaa cattgacaat tatggatggt
    14461 atagtaggta tggaaggtga tggaccttca gctggtacta ctcgagaaat aggcgtttta
    14521 ttagcatcac aaaatccata tgctatagat gttgttgcat gtaagattat aagtttactt
    14581 ccaaatagag ttgctacaat tagaggtgct attaagagag gatatataca agaagatttt
    14641 tcagatattg aaattttagg tgaagatata aaagatttaa ttataaaaaa ttacaagatt
    14701 ccaaatgtaa gtaaggattt aagattgttt tctgtaaagc taccaaaatt tttaaatgaa
    14761 cctatttcaa agcttataac accaaagcct gtggtaagat ttcaagattg cataaaatgt
    14821 ggaaaatgca aggaagcttg tcctgcatcc actatagaga tagggactaa gggtgctatt
    14881 atagacttaa gtaaatgtat aagatgttac tgttgccatg aactttgtcc taaaaaggct
    14941 attgatgtca aacgtaattt tgtatttaag tatgtaaaat aatgtataat attatatgag
    15001 tctatataaa ttaaaaatat aaaggagaaa taatatgatt ttaattaaaa atggtagagt
    15061 aatagaccct aaaagtcaaa gagatgagaa tattgactta attataaaag aaaataaaat
    15121 ttataaaata ggcaagtttg atgaaagtga tgaatatgaa aaaataatag atgctagtgg
    15181 taatgtagtt gctccaggtt tagtggatgt acatgtacac tttagagacc ctgggtttac
    15241 atataaagaa gatatagaaa gtggagctaa atctgctgct agaggtggat ttacaactgt
    15301 tatatgtatg gcaaatacta atcctattgt agataatgaa gatactttta attatgttaa
    15361 agaaaagtca aaaaatgcat gtataaatgt tttacaagca gcagctataa ctaaaggttt
    15421 tgaaggtaaa gagttagttg atatggaagc tcttaaaaaa gctggtgtac cagggtttac
    15481 tgatgatgga ttacctttaa tggatagcaa tctaattatg gaggcaatga ttaaggcaaa
    15541 agagttggat gtaccactta gttttcatga agaagaccct tctttagttg gaaatccagg
    15601 tgtaaatgca ggaaaggtag cttcagaact tggattaaaa ggtgcatcaa gtgtagcaga
    15661 agatgttatg gtagccagag attgtatgtt ggcactaaag actggtgcta aagttgatat
    15721 acaacatata agttcaggtg tatctgttga tatggttaga tttgcaaaat acttaggtgc
    15781 aaatgtagtt gcagaagctt caccacatca ctttacattg actgaggaag atgtattgga
    15841 gtttggaaca aatgcaaaaa tgaatccacc tctaagaagt aaatgggaca gagataagat
    15901 tatagaggga ttaaatgatg gtactattga aataatagca acggaccatg cacctcatag
    15961 taaagaagaa aaagatagag agtttataaa agctccaagt ggaattattg ggcttgaaac
    16021 ttctttagct cttggaataa caaatttggt gcataaaaat catcttagta tgatgcagtt
    16081 aatagaaaaa atgtctataa atcctgcaaa gttgtacaat ttaaatattg gatttataga
    16141 agaaggggct gtagcagaca ttgttatatt caatccagaa gaagaatgga cagttgaaag
    16201 ctttgtttct aaagctgata attcaccatt taaaggtaaa tctttatatg gaaaggttaa
    16261 ttatacaata tgtaatgggg agattgtata taacgcctaa tttaagatta ttttttttag
    16321 gcggagtgta caatgattga aagtaaaaga ttgtatatta ggagaatgag attgattaat
    16381 caaaattgga agtattagtt tgaacaatta gaacataaca tcttggtaat ttagatatag
    16441 taaggaagag aatccttttt atgaaagaac gcactattat atttggaaag attaagttcc
    16501 taatctatca tatataatag tgcgttatat atttaagtat gttttatata aactaatcta
    16561 aaaatcgaaa atctttatac aatttctact tataaattgc ctttataggg tctagtttag
    16621 aggctttaaa ggctggaatc agaccaaata caactccagt caatatagta gctacaattg
    16681 cataaaataa gctatttaag ctaggtatag cttcaaaacc tatatattta gaaacatagt
    16741 tagtagcagt aaatccaact atagttccta aaattccacc acatactgtt ataaaaacag
    16801 cttcaactaa aaattgaaat aatatagacc taggttttgc tccaatagca cgtcttatac
    16861 caatttctct ttgtctttcc ataacagata catacattat attcattaca ccaataccac
    16921 caacaaacat tgcaactaca gttattatag atacatattt gtttacattg gaattaatgc
    16981 tttcaagata agctgtctgt tcagttgggt ctggtgtagt ataagaacca ttgataccag
    17041 ggtgcatctc atataattta gctataacat tgtttgcaac ttcatttaca ttatagcctt
    17101 tagaagaaac taagtctaac tggtaaattt catttgaata agaattttga ctcattaaag
    17161 tgtcaaaagc ctttttaggt actagggaag tagtaaattg catatcttgg taacctccaa
    17221 agaatcctcc agcttggttt tgctgagaat catctaatac ccctattatt tcaaatatag
    17281 ttccatttat gttgatacca tgacctaagg catcttctgg attttcaaaa atttcacttg
    17341 tactttgtaa tgtaagtaaa attacttttc ttttttcatc atctaaagaa aaatctctac
    17401 cacaaattaa atttatttta gaatcttttt ttactggtcc aacatcaaca tacgtagttt
    17461 ttttatcaaa agatgcttgt gaagagtaaa ctgaatcaag gttaaaccca tctcttgaag
    17521 gagctattct ttctactcct tcaacaaatg ataattcttc taaatctttt gcattaaatg
    17581 gttttaaaaa aatactcata tcatcagtaa gacctgtgtt atcggctgat tcaaacgaaa
    17641 tagtagtttt attagggttt acattgttta cagatttctt aatttctttt tgaaatccat
    17701 tacctataga actaacaagt attactgaag ttattcctat tataatccag agtaaagcta
    17761 caaatacacg aagcttgtga ccttttaaat tggccaaaga attttttaat aaactcatga
    17821 ttacacctcc gaagtaaaca caccatcttt aagtcgtata accttagttg catacttagt
    17881 taaatcttga tcatgtgtaa ccattattat agtcttacct tgtttgttga gccttgttaa
    17941 aagctccata atttctctgc tagtttcact atctaatgcc ccagtcggtt cgtcagcaaa
    18001 gattatctga gggtcatttg ctaggcaacg tgcaatggct acacgctgtt gttgaccacc
    18061 agataattga agtggtttct gcttaagctt gtccaaaagc ccaacagaac ttaatttatc
    18121 tttaacaatt tcttctctat tagatttttt tagtgcttta ttgtaaatta gaggcaactc
    18181 tacattttga tatacattta gagtttctat taaattaaat tgttggaaaa caaatccaat
    18241 attaatattt ctaaaaacag aacgttcatt ttcactcaaa ttagttacat ctgttccatc
    18301 aaatatatat cttccttcat caaatacatc tagaaaacct aagatattaa gtaaagtagt
    18361 tttaccacta ccagacttac ccattatcat gacaaattct ccagattcaa tttctaaatt
    18421 taaggatttt aaaacatgta attcatcttt ccctactttg taatattttt gaatattttc
    18481 taatttaatt aacatatgta aattaattca catccatagt aacttcttct acttttttgt
    18541 ttggagtagt atctccatta ttagaaccag cagttacgcc atcgccttga actgggtctc
    18601 cttctttcat ttctttagta gggtgtttta ttattatatc gccttgttca agaccgcctc
    18661 taactacagc aaaatcatca ttttgacttg ctacatctac aatttgcttt ttcaatatac
    18721 catctaaatc cttaaataca tatgagtgtt tgttgtcttt aagtacacaa gatgctggta
    18781 ttttaaagct tgaattagct acttcaagtg atgcttgaag atggaatcca tttactaatc
    18841 cttcttgatt atcaaatgct atgtttatat cataataaga tagtgtattc tgctgagctc
    18901 ccatttcagt atttggagta gatggtttat cagatataaa acttattcta ccagtaagct
    18961 tttggtcagt tgaaaaaact agtatattga ccatttggtc aatcttcatt ttaggtaaat
    19021 cttgttcact tgcttgtcct ttcatataaa actctagacc ttgaatagtc ataaatgaaa
    19081 ctggttggtc tggatttcca gtttggtcat tcaagtatac tgtgccatca aaaggagcag
    19141 taactctgtc ataagctttt ttatccagag aagctatctg ggagcttaaa gcagttatct
    19201 gtgcatttat agaagcaata gcatctttat tttcagcaat atcagaaagt gatatttttt
    19261 gttttttgta ttgactaagt tgtgatttta aactatctat ttctgctatt atagttggat
    19321 ttttagcagt aaaaagaagg tctccttgat ttactttttg accatttgta acattaacat
    19381 cacttaactc ataatctcca gatatattga agtccttagt ttgcttagga acaaccatcc
    19441 catttataaa tattttttca ttttcaggaa tagtatatgt ttcgataaaa ttttcattag
    19501 cttgtaattc agtgtttttc gaatcaacat aagcaagaac tcctagaatt aaaagtatga
    19561 ttactgctat tcctgcatat attttgaatc tcttattttt aaagggattg tttttaaaac
    19621 ctttattttt agacttgaag ccactacttc caaaactgct atttctaaag ttgttacttt
    19681 taaaactgtt atttctaaat cttttatttt taaatttttt tagaaaagac atgtcttcct
    19741 cctttttata aaaactactt aattatatac tgggatgtac atatagaatt actataagaa
    19801 gttgaatata agttaatgaa aataaactat caataataag atactataaa tcacatttga
    19861 atgtaatagt aaattttgta tattggttgt actctgaatc tacatcaatt ttatgaccaa
    19921 gcttatcaca aagttctttt actaaataaa gcccaagacc tgtagattta gaattgtttc
    19981 ttccattatt acctgtaaat cccttattga atattcgatt taaatcttcc ttttttatac
    20041 cacaaccagt atcttccact cttaattgta aataattttt ggttcttttg caatagatag
    20101 atatattatc tcctaatgag gagtatttta gggaattact caataattgc tcgaatatga
    20161 agtatagcca tttttcatca gataaaacct taaaattcaa attatttaag tctaaaataa
    20221 ttttttttga aataaaatat tttgagtatt tttttataat atttctaatg attttttcaa
    20281 tatttacctc ttgaattaaa aaatccttat gaaaatcttc gagtcttgat aaatataaaa
    20341 ttttttcaac ggagttttca attctaaata tttcactatc tatgtcatct gaaatttttt
    20401 catcaattaa attttcattt tgttcaagaa tgagttttat tatagctata ggtatcttta
    20461 catcatgtac ccacatattt aaaatctctt tgttttcttc aaatttctcg atattattat
    20521 ttattatgtt attacattga tattgatgat ttttaataat gttcaagtat attttttctt
    20581 catttttaca tctagctaat tctatatcat tcatattaat tttgaatgta tcatttttta
    20641 tcttatctaa aaatttgttc ttcttgatgt agctaataaa tagatataaa aataaaaaca
    20701 caacatttac aactaatata tataaaatag tatcagtcag aaaaacatct acaggagaaa
    20761 atataataac tgatactgta aaaattaaga aagtaaaatt gtatactaaa aaacctagtt
    20821 tatccttgag aaaatcttta aagctcataa attcacctac ttagaattgt tttaactgat
    20881 tatataaccc aatcctcttt ttgtttctat aaaatcttct aaaccaattt catttaactt
    20941 tgagcgtatt ctatttacat tgacagttaa tgtattatca tctacaaacc aatcattatc
    21001 ccataatttt ttcatgagtt tattcctact tactatctgc cccttatatt tcataagctc
    21061 atgaagaatt ttgatttcat tttttgttag ctcaatagta ttatcttcat agtttattgt
    21121 tgcagttgat aaatctaaag taacaccatt gtgaataatc tgatttgagt tattatcaac
    21181 aaaattataa gttcttctta aaagggcatt aactttagct actaagacat ctacagaaaa
    21241 aggtttttgt atataatcat ctgcacctaa atttacaccc attattacat ccatattaga
    21301 atttcttgaa gaaataaaaa ttataggaac ttttgaaatt tttcttattt cattacacca
    21361 atgaaaacca tcataaaatg gaaggtttat gtccaataaa actaattctg ggttatagtt
    21421 tttgaactct tctattatat tttcaagatt ttctatttga tgaacatcat gtccccattt
    21481 agtaagacac tttgctatga tatttttcaa tgagacgtca tcttcaacta ccattatttt
    21541 aaacattctt acctcctaat tcaaaatagt tatattaaca taactatatt ctacttgata
    21601 aattaaaata tttctacctt attacctaaa ataattatat taatataact atattttact
    21661 tgataaatta tattttaaac ataattgcaa taaatttgaa gaaatataat taatacttac
    21721 gaaaatgtaa ccttaaatta atataattaa atgtttaaaa gccattttaa tatatataat
    21781 atatttgtga gaattgataa ataactaatt gtgatgaaag gtgattatta actatggaag
    21841 aaatattatc tgttgaaaat ataaaaaagg agtatggaag aaaaggttca aaacatgaag
    21901 ctctaagagg aataactttc aaggtttata aaggagaatt tgttggtatt atggggtctt
    21961 ccggtgctgg taaatcaaca cttttaaata ttatttctac aatagattta ccttcatcag
    22021 gagatattta tataaatgga aagaatacta ttaaaatgaa gcaaaatgaa ttggcagatt
    22081 ttagacgtga taatctaggc tttgtatttc aagattctaa tcttttggat acacttacaa
    22141 taaaagaaaa tataatgttg cctctttctt taaaaaatga aagagtatca gtaatagaaa
    22201 atagaataaa agagatttca aaagaattaa atatagaatc tatattagat aaataccctg
    22261 gcgaagtttc tggtggacaa aagcaaagag gagctgtatg tagagctata gcaacaaaac
    22321 catcattagt acttgcagat gagcctacag gagcattaga ttctaaatct gctagggatt
    22381 tgttgaattg tctattaaaa ttaaacaaag acagcaataa aacaatattg atggtaactc
    22441 atgatgctat aagtgcgagt ttttgcaata gaatactctt tataaaggat ggaataatat
    22501 tcacagaaat tgtaaaggga gaaagtaaca gagagttcta taataaaata gtaaatacag
    22561 tttccttaat tggaggagtt aataaaaatg actttattta gaattgcaat gcaaaatgta
    22621 aagaatagtt tttttaatta tttaatgtac tttatttcaa ttgtatttag tgtatttgta
    22681 ttttttagtt ttaaatctat agagtataat gaagcgttga gttcacttgg agagaaaact
    22741 aggatgagta ttaatacaag ttcaattgta atagtggtat ttgtattttt atttatatat
    22801 tattcaaact catttttttt caatagacgt aggcgagaag taggaactta tagtttactt
    22861 ggaatgagga aaaatcagat aggaaaaata tttttatatg aaactttttt gatgggtata
    22921 gttgcaatac taataggtat atttttagga ctgttatttt caaagcttat gactatgatg
    22981 cttgtaaaat taatgaatga aatgatagta gtaaatatga acttaagtat aaaggcctta
    23041 gttcagacac tagcggtatt tttaataata ttcatagtta ttggaataag aaatactata
    23101 gttatcagaa ataagagaat tatagagtta tttaataaat atccagaaaa gtataagttt
    23161 aaaaaattta taaagttaaa atgtgtactt ggtgttttat taatagctat ttcttattta
    23221 atgtcaatta gttattttat tgctgaaaat attatgcttt cagtttttat attagttaca
    23281 ataatacctg gaactttttt atttttttca tcaacgatgt atatgattat tgatgctgtt
    23341 aagaagagaa aaagttttta ttataaggga cagaatttaa ttgcattttc tgaacttgga
    23401 tttaaattaa agagcaatag tagtgtattg gctgtaatag caatattaat agctacaagt
    23461 gtaactatgc tagggtttac catatcactt tattacgata tagataggaa cataagtgaa
    23521 aattataaat acagttatac cataaattta ggaaattctt atgtaaataa cgaaattgac
    23581 aaattattat ataaatataa aaaagataat aagataatct ttgataaaac aatagaactt
    23641 atagacaaag atataaatat tgaaatgtat tataaaaatg gaaatagtta tatagaagat
    23701 agaacgtctg tagatattat tagagaaagt gattttaaaa aattaagaca atatcagaat
    23761 aacaattatg aagaattaac ttcaaaggaa tctgtgtact atgtttctga ttcatacaaa
    23821 aaaatatttt ttaaagactt tgaggttgaa aaaataaact tgcatattaa agatacaaat
    23881 gagtatgata taaatctgtt taaagtacaa aaaagcatct tggagccaga aataaattca
    23941 caagctacgt ttgatttaat tgtagcaaaa gatgacgtat ttaatcagtt aaaattacat
    24001 ggaaattcta gattaataag attaattgat ataaaaaatg aaaaaaaaga actggatttg
    24061 actttaaaat taaaaaatat agtaagtgaa aatatgaaat ttacttatcc ttttaatttt
    24121 acatcaagta ttgaaagtta taataacttg ataaaattaa gtggtctaat gctgtttata
    24181 ggcatatttt tgtctgtagt atttctttta tgtacaggta gtataatctt atttaaacaa
    24241 ttatcaaata tatatgatga taaggaaaga tatattatgt taataaaact tggagcaaat
    24301 aataaagata ttgagaaaat aatatcaaaa cagttaaaag ttatattttt aatgccatta
    24361 gttgtaggta cagttcataa tttatttgct atgtctatag cacaaaagtt tataccaagg
    24421 tcattacttg taccaattat cataactcta gtcatttatt ttataggata ttttatatat
    24481 tattttttaa ctttaaaata tgctctaaac atgataaaag aataaattat ttttaagtca
    24541 aaagaattag atatatgttt tgaataaaat aacttattta agtgcagata gaaaatacta
    24601 gaattgagtt taagatattt atttaaagac aaaattaaag ttaggaaaaa gtctatattt
    24661 tatatcattt tgtatcagtt acattacttg atgttatata ataaatctaa gcagaatatc
    24721 aaataaaaaa tgacgaggag ctgaaattta tgaagtttaa tgcctaccaa cataaatact
    24781 catcaataag aaatgtcgtg tatgctaaaa atggagcagt tgcaacatca acaccattag
    24841 catctcaagc tggacttgaa atactaaaaa agggtggaaa tgctgttgat gcagcagttg
    24901 caacagccgc cacattagca gtagtagaac ctacaagcaa tgggattgga ggagacgcat
    24961 atgcccttgt ttggatagag gaagaaaaac gattgtatgg tttaaattct agtggctttg
    25021 ctccagaaaa tatggaatta aaaaattata ataatatgaa agaaatgcca aaatatgggt
    25081 ttggagcagt aactgtacct ggaataccag cagcttggtc agaattaaat aaaaaatatg
    25141 gtaaacttag cttaatggaa tgcttatctc cagcgataaa ctatgcaaga gagggatacg
    25201 tagtatctcc taatgtagct aaggtatgga aaaaatcata tgaactctat gaaaaagaac
    25261 ttattggaga agagttcaaa ccatggtttg atacattttc taaagatggt aaagctccag
    25321 aggctggaga tatatttata tgtgaagaac aggcaagtac attagaagag atagcaaata
    25381 ctcaggctga aagcttttat agaggaagac ttgctgataa aatagatgaa tattctaaaa
    25441 aatttaatgg ggctataaga aagagtgact tagagtgttt ttatccaaca tgggtagaac
    25501 ctataagtac agaatataag ggatataaaa tatttgaaat cccaccaaat ggtcatggaa
    25561 taacagtttt aatggcactt aacatattaa aagaattgga attagaagga aatatagaaa
    25621 atgtagaaga catacataaa attatagaga gtttaaaatt agcttttgca gattctaaaa
    25681 catatgtaac tgatatagag catatgaagg taaagattca ggaattacta agccaagagt
    25741 atgctaaaaa aagaagttta ttgattgaca ataaagaggc tttgtatcca actgcaggag
    25801 agccatattg tggtggtaca gtataccttt gtactgcaga taaggatgga aatatggttt
    25861 catatataca gagcaattac ataaattttg gttcaggaat tgtgatacca agaactggga
    25921 ttgctttaca tagtagagga aataatttta atttagaccc taaacaccac aatgtagtaa
    25981 aaccatttaa aaaaccttat catactataa tacctggatt tttaggtaaa gaagataaag
    26041 caataggtcc atttggtgta atgggagctt ttatgcaacc acaaggacat attcaagtgt
    26101 taacaaatat gattgatttt ggattaaatc cacaagaggc gctagatgct ccaagatggc
    26161 agtggataaa aggaaaagaa atagaagttg aaccagagat gccaaagcat ataatagatt
    26221 cattaattga aaagggtcat gaaataaaag taattcatga tactgtcgac atgggacgag
    26281 gtcaaataat atttaaaaca gaacaggaaa gttatatttg tgggacggaa tcaagatgtg
    26341 atggtcatat tgcagtttat taatattatc aactaaaata tatataataa atttaaagag
    26401 tatatgaaat aataagtaaa ggcgaaagtc tacaatgact gacgccttta caaaatttaa
    26461 gctatcatat gctgatttta tatagattat gagccttatt cttgaccagt tttgatttca
    26521 ttgtcatcat aactaatcca gtcactaaag ctaccaatat aaatcttgtg aggtatatcc
    26581 agttctctaa gagcaagact gttaacacaa gcagatatcc ctgaaccaca agataaaatt
    26641 acttcatcat aattatttaa atctttaaag aattttttta aaaattctat atctttaagt
    26701 gagccatttt caaagtcact ttttattaaa tccttacaaa aataattttt tgcacttggt
    26761 atatgaccag cttttgagta agcaggttca actaaaccct ggtatctctc attagcacgg
    26821 caatctatta ttacagtatc ttttttgtat aatttagatt taacatattc cattggaaca
    26881 actaaatcat tgtttatatt tggtctaatt tccttacctg tacaacttgg tatattcact
    26941 ttctcttcca attctccacc ttcctttacg aaagatgtta ttcccccatc tagtacatag
    27001 acatttttaa gccccaaatg ttttaattgg aaaaataatc tgcaagctcc atttaaatct
    27061 ccatcatcat aagttacaat cgtagtgtca ttatcaacac ccattttttc taatttttct
    27121 tttagtatta aagggtcttg taaaggattt ttaccaccat gttcttttgt aggagatgat
    27181 aaatccttgt ctatatctaa gataaaactg cctttgatgt gtcctttttt ataagaatca
    27241 ataccataag ttctattgat aagatcaaat ctacaatcta ttacaactag attatcattt
    27301 ttttctagtt ttgatataag ttcttttgct gaaataatgt ttttcaaaga atgtacctcc
    27361 tgtgtttata ttatctttat tttaatttat attaattatt aactttataa actaatatta
    27421 ttataattta ttacataagt aaataaatct ttagaaattg taaaaatttt atataaaaat
    27481 tcaaataatt aatgaatatt gaaaatttta gatatataat aaaataagag tatattttat
    27541 acaatatgaa agctggtatt gcaatagatt aaagccttat acacgtttag tttttaaaga
    27601 aattagtgtt attatgaaaa cctaagttaa tttattaaat aaataattga ataaaaaaat
    27661 tttattatgt atttataaaa tttttgttac aaatttggca taatatagta tactttaagt
    27721 gtgtattagg ttaaatctat aacaaattag acgtatgata tagacttagt gtttattggc
    27781 atttacaaga aatttgttaa tatcaactat ttgtagtttg ggcagttata attattatag
    27841 gggggatgtc caatacaaaa caatcaacca agaactaata ttatattata aagagttata
    27901 gttttgtgtg tttatgtttt taattaaatt taaagtggga aaaatatatt tgttagaatg
    27961 gagaatagcg agatgagtaa taaaatagtt agtaagagac aattaacaga tagtatttat
    28021 ttaatggaaa tagaggcacc tagagttgca aaatcttctc aaccaggtca atttataata
    28081 attaaaaatg atgaaaaagg agagaggatt cctcttacaa tagctgacta tgatagagaa
    28141 aaaggaacag taactattgt attccaaaca gttggtgctt caactaaaaa gttagcaatg
    28201 tttgaagaaa acgattttgt aatggatttt gtagggccat taggacaagc aagtgaattt
    28261 atacatgaag atatagaaga attaagaaat aaaaagatat tatttgtagc tggtggagtt
    28321 ggttctgctc cagtttatcc acaagttaaa tggtttaaag agcatggatt agatgtagat
    28381 gtaataattg gtgcaagaac taaagaatta attatattag aagatgatat gaaaaaagta
    28441 gctaaaaatg tatatgtatc aacagatgat ggtacatatg gatttaatgg aagagttaca
    28501 gacctattaa aagatttagt tgataatcaa ggtaaaaaat acgaccaagc aatagtaatt
    28561 ggaccaatga taatgatgaa atttatgtgc caattaacta aagaactaaa tatacctact
    28621 atagtaagtt taaatacaat aatgatagat ggaacaggta tgtgtggtgg ttgtagagtt
    28681 agtgttggaa atgaaactaa gtttgcatgt gtagatggtc ctgaatttga tggtcattta
    28741 gttgattttg atcaagcaat gagaagacag tctatgtaca agactcaaga gggaagagct
    28801 atgctaaaac ttgaagaagg cgatagtcat catcatagta attgtggatg tggaggtaac
    28861 aaataatgga tgctaaaaaa gtaaaagtac cagttagaga acaagaacct gctgtgagag
    28921 caacaaattt tgatgaagta tgcttagggt ataataaaga agaagctatg gcagaagcta
    28981 atagatgttt agcttgtaaa aaaccaaaat gtgtgggtgg atgtccagta ggaatcgaca
    29041 taccagggtt tataacaaaa attaaagaag atgatataga aggtgctgct aaagtaatag
    29101 ctaagagcag ttcattacca gctgtttgtg gtagagtatg cccacaagaa agtcaatgtg
    29161 aaggtgtatg tatacttgga ataaaatcag atgctgtatc aataggtaaa ttagaaagat
    29221 ttgtagcaga ttggtcaaaa gaaaatgaca taaatttatc tgatacagag cctaagaaaa
    29281 atcaaaaagt agcagtaata ggaagtggtc cagcgggtct agcttgtgct ggtgatttag
    29341 ctaaaaaagg ctatgatgta acaatctttg aagcaatgca tgagccaggt ggagttttaa
    29401 cttacggaat acctgagttt agacttccaa aacaagctgt tgttcaacct gaaatagaca
    29461 atataagaaa attaggtgta aaaatagaaa ctaatgtaat tgttggtaaa acaattacag
    29521 tagatgaact tatagaagat gaaggatttg aagctatatt cataggttca ggagctggtc
    29581 ttccaatgtt tatgaatata ccaggggaaa atgcaaatgg agtattttct gcaaatgagt
    29641 ttttaactag agtaaactta atgaaggctt atagagatga ttatgataca cctattagtt
    29701 ctggtaaaaa agttgctgta gtaggtggtg gaaatgttgc tatggacgct gctagaactg
    29761 cattaagatt aggttcagaa tcatatatag tttatagaag aagtgaaaaa gagcttccag
    29821 caagagctga agaagttcat catgcaaaag aagaaggcat tatatttaat acattaacaa
    29881 atcctaagga aattttagta gatgaaaatg gatatgttaa aggtatggtt tgtataagaa
    29941 tggaattagg agagccagat gattctggaa gaagaagacc tatagaaata gaaggttctg
    30001 aatttgtact tgatgtagac acagttataa tgtcacttgg aacaagccca aatccactaa
    30061 tatcttctac aacaaagagt cttgatataa ataagaagag atgcttaata acagatgaaa
    30121 atggtcagac ttctaaagag ggtgtatttg caggtggaga tgctgttact ggagcagcaa
    30181 cagtaatatc tgcaatgggt gctggtaaaa cagcagcagc atcaatagat gaatatttaa
    30241 aagcaaaagt taatgcttaa tttgtaaatt aaagagaggg tgtctcaaaa tgaacttttt
    30301 agttcatgag gcactctttt tgtacaaaat caaatatgtt ttaatcattt caacaatgaa
    30361 aatttcattc ctgtagaaag tctaactttt aggtcttagt acaatctcta ttttgatgta
    30421 gccacttttt tagatggttt aataatttat cagttattat tgtaatattt gatactaagt
    30481 aacataactt tgtctaacta tgataaaata gttatacaat aaaggtcttg taaggagtga
    30541 ataatgtgtg gctaataaaa tcagatttgt caaatctaac attaaataat agaaaaataa
    30601 attgtgtaat taatggtatt ttaatatgaa aaaaagagaa aaagtatttg atgggctagt
    30661 ttttaagcat atattaatct tattaatact attagttttg attgatttaa caagtaccat
    30721 catatatgca agtaactcta tggaaattac ttctagaaat atgattgagg catcaaaaag
    30781 agaacttgaa aactatttag aagtaaacat atctttatta aaagctctta gtcaagatga
    30841 tagattttct aatggtgaaa catcactaat tgaaaaggga aaattattaa gtccatatca
    30901 aaaggaatat aatcttttta tgattggtat aacagataca aaaggaaatg cttcaagtac
    30961 atatagggaa aatgttaact caataaaaga taagcctagt tttgagaaag caattaaaac
    31021 aaagcaagta gtagtttcag atattgaagt cagtaatgta acaggtgaca aagtatttat
    31081 catatatgta ccaattgtaa aaaataatga gatgattggg accatttttg cttcttttta
    31141 ttttcaagat gtaaataatt taataagtag aagcaatttt gatgattcta ttaaatttct
    31201 aatgatagat aaagattata ctattatttc tcatccaaac aaaaaatatg taaatgataa
    31261 aagtaagatg cttgatttag aagggaatat tataggaacc actaaaagtg aaatcttaaa
    31321 aaatataaat aaaaaatgtc aaggtgattt tttatcttgg gataattgga gattatataa
    31381 tgtaaaatat acgaacataa agtggactaa ttggacttta gtatcaaaat gtaatatatt
    31441 taagaacttt aaaaatctaa tagttaattt tatgataaag ttgtattttt atatagtaat
    31501 atttatgata ttgtggaaat taagtaatgc taaattaata gaacaactta aaaaattggc
    31561 ttattatgat tctttaagtg gaataaaaaa taaagaaaag tttagaaaag attctatgta
    31621 tattttaaag aattattatc aagataattt ttacttagta caattggatg tcaataaatt
    31681 taaatatata aatgagatgt ttggatatgc tgaagggaat aagatattaa ttcatatatc
    31741 acaggtttta aataataata caaataaata tgaaatttgt gctaggatgg ataatgacca
    31801 ttttatattg ttgattgctt gtaacactga agatgaactt ttaaatagat tatctaaaat
    31861 aaataaagaa atttgtaact taaatactac taactcatct aagtataaaa ttgttatgtc
    31921 aagtggaata tataagatta ctaaaaagga tgatattaaa aagattgact tgttaattga
    31981 tagagcaaat attgcagcaa aaagtaaaaa ggaaaaatat gaacattcat attcattttt
    32041 taatgaagat acaagaaatc gattgtacaa ggaaaaacga ttggaagata atatgaataa
    32101 agctttagaa aaaggtgaat ttatagtata ttatcaacct aagtatagtt tagatgatgt
    32161 aaatgaaatt gaaggagcag aagccttgat aagatggaat agtccagaat ttgggtttat
    32221 ttcacctata gattttgtac ctttatttga aaagaatggt tttattgtaa atattgatat
    32281 gttcgtgttt gaagaagtat gcaaaacatt aaacaagtgg ataaataaag ggtatactcc
    32341 tgtaccaatc tctgtaaata tgtctagagt tcatttatat agggataatt ttatagaaaa
    32401 tataactgat ttgataagta aatacaatat atctcctgag tttattgaac ttgaactaac
    32461 agaaagtgtt gtgtttgata atttaaatat attgattgat ataatgaaaa agattaaaga
    32521 gataggattc cttatatcaa tggatgattt tggttcggga tattcatctt taaatctgtt
    32581 aaaagacctt tcttttgata ttttaaaatt agatagagga tttttaatcg aaacaacaga
    32641 tacaaaacgt ggaaaaatta ttatttctaa gattgtggaa atggcaaaag cgattgatat
    32701 taaagtaatc tgtgagggcg tagaaactta tgaacaagta gagttcttga aagagatagg
    32761 ttgtgataag gttcaaggat acttatttgc taaacctatg gtattagatg aatttgaaaa
    32821 acacttaaac tttaaatttg attaatcatt aataattatt agactataat ataccctaac
    32881 aacttactta caaatatgta agttaattgt tagggtattt ttatggttaa agtagtatat
    32941 tcacgatatt atggtattgt agtaaaaata aaaaattatg gagaatataa aatgaacata
    33001 ttgagtatta gaaatatatc aaaaacatat tgcggaaata ttccatttaa agcattagat
    33061 aaggttagtc taaatataga aaagggagaa tttgtgtctg ttatgggacc atctggaagt
    33121 ggtaaatcta ctcttttaaa tataatatct acagttgata gacagagtga aggtgaggtt
    33181 gtattagatg gatatgatgt aagtaaattg aaaggagaaa aacttgctga atttagaagg
    33241 aaacaacttg gttttgtatt tcaggatttt aatttgattg acactttaac tgttggcgaa
    33301 aatattatgt tgccacttac actagagggg gcaagtatca aagatatgaa tatacaaact
    33361 aaaagtatat ctaaattttt aggaatagat aaaattttag atagaaaaac ctatgaaatt
    33421 tcaggaggtc aagctcaaag gtgtgctatt gctagagcta ttataaataa acctgccata
    33481 cttcttgcag atgagccaac tggaaatctt gattcaaaat ctactgatga tgttttgaaa
    33541 ttatttacta ggataaacaa agaacaaaag gtaacaacac ttatggtaac tcatgaagca
    33601 tatagtgcaa gttattcaga tagagtaatt tttataaaag atggttgtat atatacagaa
    33661 ataaaaaaat ctgaaagcag taattctttc tattctgata tactagctgt tttgtctcaa
    33721 ataggaggtg taagatagtg gattttagaa agtttgctta taataatata gttaaaaatt
    33781 tcagggcata tttagcatat tttttaagtt ctgttttttc tataatgata ttttttgttt
    33841 ttgcagtgtc tatgttccat ccaattataa caaaatctgg aatgcagagt ggttctactg
    33901 catttatggc tcttatgact tcagaattaa taatattcgg ttttagtcta ctttttatat
    33961 tgtactcttt aggaaatttc ttgaaatata gattgaaaga ttttggagta ttgatgataa
    34021 ttggaatgag taacagacaa ctgaaaaaat tgatattaat tgaaaatttg ataataggat
    34081 ttttagcagt gatgttagga atattacttg gtttatcaat ttcaaaattg tttttattgt
    34141 atttaagcaa actgttttat atgaatttaa ctgaatttta ttttccagta aaagcaatgc
    34201 tattgactat tgtatcattt atgattttat ttttgataac aggaccaatg agcttaaaat
    34261 tattgagtaa aaagagcatt ttagagcttt tagtaggtac aaaaaagcct aaaaaagaga
    34321 taaaatcttc aaagctattt ggaataattg gtgtaatatg tcttatatta ggatatgcta
    34381 tgatactatt tggcaataag ttaaaaataa atggaggtat gtatacaaca gcactattaa
    34441 ttactttggg gatattttta tttttttctc aatttactat tttgatatta aagtatttaa
    34501 aaaataaaga aaaattttat aagaacaaag taaatatgtt acttataagt aatttgatat
    34561 ataaattaaa ggataataca cgattgttat ttttaataac aatactttta tcggctactt
    34621 tagtagcatt tacaacaact tcaacacttg taagttctca aggagaagct tctaaaaata
    34681 attttccaat ggtttactca tatttttcag agcataataa tgaaaaagaa tatgaagaac
    34741 tacaacaaat tagagaagca atcaaaggat atgattataa ggagttaagt tttcctgtac
    34801 ttaaatataa tagggaagtc ttactaagtg taaagaatta taacaaatta tcaaaggaat
    34861 tgggattaga taaaattagt tcaaagttag aaaaatatga aggggttata attcctagta
    34921 gcaattcaca agaacatata aattctttaa aacaattaaa aaattatagt ataaaaaatt
    34981 taaatataga agtaaaaaaa tcaaaaaata aaataatttt tcctacagga ctgtttagta
    35041 aagttgtagt tataagtgat gacttataca atgaagtaaa aggtgaattt tcaaaaatta
    35101 attttcatgg atttgattat aaaaactggc aaagcagtgc acagataact gaaaatttaa
    35161 aagaaaaaca ttttaatttg gatagagaat ataacaggtc atatttttta acattaccag
    35221 atatgtattt agtagagtta caacaaagta aaattcttca atttatgggt gtttttatag
    35281 gagtgatatt atttatagca gtttttagtt ttttatactt tagattatat acagacgaga
    35341 tagaagataa tgtaaagtac aaaaaactat caaaaatagg tctatctttt aaaaatatga
    35401 ataagattgt tagtatagag ataggtacat tgttttttat accgtatttt atagcaatta
    35461 taaatagcat aatttcttta ctgtttttaa atcaagtatt aaataatagt tttaagttag
    35521 aaaatttggg tatattacta ctgatatcat ctatatattc actttatttt tacttattaa
    35581 aaaaaattta tactagaaaa gtttggtttt ttgattaaag tggtatataa tagatatata
    35641 aattaatatt taaaaagtag ttaatattag gggagggcat ttgcacctcc cttgtaatat
    35701 gtggaaaatt gaatattaga agaaggagga gacttgatgg aaaatcaaca attattagga
    35761 acagaacgaa ttagtaaatt actactaaaa tattctatac cagctataat aggtatgttg
    35821 gtaaatagtt tatataatgt agtagataga atttttattg gaaatatacc aggggttgga
    35881 ccacttgcaa taacaggtct tggtgtaaca atgccaataa tgacaataat acttgcattt
    35941 gggatgttga taggtatagg aactactact accatatcaa taaaacttgg gcaaggtaaa
    36001 gtagaagagg caagaaaact tataggaaat gcaatgacgt tatctgtaat aacaggaatt
    36061 atcataatga tacttggaat attatttgca aataaaatac ttacattgtt tggagcaagt
    36121 gaaaatactt taatatatgc aaaatcatat atcaatatta tattattagg tacagtagta
    36181 aatcttcttt ccttttcttt aaaccactca ataagagctg atggtagtcc taaaatttca
    36241 gcaggaataa tgatagtagg atgtttaact aatatagttt tagactggat attaatattt
    36301 ggattcaatt tgggaataca aggagctgca attgctacag taacttctca ggctcttaca
    36361 gctattttaa ctatagggta ttatataagt ggaaaatcaa acttacgatt cagtaaatct
    36421 aatctaaaat tagataaaaa gctaataaaa gcagtgtttg caataggtat gtcgccattt
    36481 gctatgcaac ttgcagcaag tttagttcaa gtaatatcaa atattgcact taaaactcat
    36541 ggtggagatt tagctatagg ggctatggct actatttctt ctattgcaat ggtatttttg
    36601 atgcctatct ttggaataaa tcaaggagcg caacctatta taggtttcaa ttatggagct
    36661 gaaaaatatg atagggttaa aaaagcatat ttaggttcat tagtagtagc tactataatt
    36721 ttatgtatgg gtatggtagt agttatgctt ttcccagaaa ctattatagg tatatttaat
    36781 aaagaccctg aacttatgaa tatatctgtt aatggattaa gaatatactt acttatgttg
    36841 ccaattgtag gcttatcagt tacaggaaca aactttatac aatctatagg aaaagcaaaa
    36901 atggctatgt tattgagcct tttaagacaa gtaattttat tgataccagc agtattaatt
    36961 cttcctacat tcttaggttt acagggtgta tggacagctc aaccagtgtc tgattttata
    37021 gctacagtaa ttacaggtat agttgtattt agagaattaa aaagatatac tcctaaaact
    37081 gaaaaattaa atgaaaatga aagactaaac gagataacta ctgagtaaaa atataaattc
    37141 agataattga gcaagaaatt gcaaaaaata cgattatatg ataaaataaa accgctgtca
    37201 aggataagag aaattctaat tatggagtga ttttatggaa tttacaaaga aaagcaaatt
    37261 aaaaaatatg tatgtaaaca tgaatgtagc tagaatgttg tctaagataa atgagtataa
    37321 gggtagacaa ctgctttaca aaaaacaacc aaaagaaatt ttagaaaact tagaaaaaaa
    37381 atctttagta gattgttctg aatctacata cattggaaat caaagagata gtagtaattt
    37441 taatctagaa aagttgataa gtaatgaagt tacacctaga agtagagagg aattgagtat
    37501 tgttgaatac agagatgtgg taaaaactat taacagtgct tatgagagta ttcccatcag
    37561 ttcacaaacg attcttgagc ttcatggata cttatataaa ttttcatcta ctagaggagg
    37621 aagttataag tcagataatg attttattga acacaattta aaacctagcg aatttataag
    37681 tattgattca agtgtcaata aagttgaaaa agctgtagaa gaaatatgtg aagcttataa
    37741 tacattgata gaggaagatg aaattgatat attaatatta atttctgcat ttgtgttaga
    37801 tttcatattg atacatccat tcaaagaagg taatataaaa atggcgagag tacttatcct
    37861 attgttacta aataaaaatg ggtatgaagt tggaagatat ataagtttag gaaagatatt
    37921 tgatgatagt tcatatgaat attatagtaa tttaaattat ttgaaagcct caataggtag
    37981 tgaaaaagca gatatgaatg catggattga gtatttttta gagacgatat taactgcata
    38041 tgagaagctg gatgatagtt taaatatatc tgataagaaa agacaaacaa aaactagtag
    38101 aatagagaaa ataataaatt ctacccttgg atattttaca aaagaagata taagagattt
    38161 atgtcctgac attccagaac ctactataaa tagagttttt aataatctaa gaaaacaaga
    38221 taagatagaa gttgttgcaa gaggaagaag tgcaaaatgg aagaaaaaat attaaaaaat
    38281 gtttagataa ctaaaatggt atagtgtttt acactatacc atttttaatg tcattagcat
    38341 gagcaataat ttaacaactt aagcaatatc aattttattt attgattatt tttgaactga
    38401 aaaatggcgt tatatggtat acctcagtgg taattaattt tggatgagaa gatataatat
    38461 tacagaatat attaggagta tatgtagaaa aaatataata aaagtgtgag gcttagtaat
    38521 taacaaatta tgtggcttca cactttttta taaaataaag atacttagca gaagaatatt
    38581 ttagttgata tgtttataag atggtacttg tttgggtaaa agtataaata gtaaaaaagt
    38641 agaagtaatg tgtataaaaa cgtaagttac attctgtaat aatagttatt ttataattca
    38701 acaaaagaat agaatgggta tatttgtgaa ataataatat aaaataatct taggagtgag
    38761 cttatgaaga aaaaattatt atatataaat gtaaattcaa aacctgaaga cttatcttca
    38821 agtaaaactg tagcaagaaa atttataaat aaatttatgg aaaaaaataa agattttgag
    38881 gttgaagaga tagaccttta taaagaacat ataccaagac ttgagtatca gtactttgaa
    38941 aaaagaaata gcatagttag tgaagaaaat gctaaaaatt tagacgataa ggatagaaaa
    39001 gaacttacaa agattagaaa tctttgtgac caatttgtga gtgcaagtgt atatgtaata
    39061 gcagcaccta tgtggagttt atcttttcca gcaccactta aagagtatat agattgtata
    39121 attcaagatg gaaaaactat atcttttgaa ggcaatgata aaccacaagg aattttaaat
    39181 gatattgata gaagtatggt atatatacaa tcttctggtg gacacatacc atgggtgttg
    39241 aagccagtta tgaataaagg gttaaattat gtggaaagta ttatgaaatt tataggaatt
    39301 aaaaaatttg atgaattgtt agtagatgga actggaacta gtgaagaaga aagacaagct
    39361 gctatagaaa aagcagctga aaaaatagat ggtataatag atgggatgaa attttaatta
    39421 taatatttta ttaaaaatat aaaggaaaaa cctcttttaa tgtagaaact tatattgaag
    39481 gaggcgagaa tcatgcgaga agaaaaaagt aatgaaaagt atgattgtta ttggtgtaat
    39541 caagagaata atttctgtgt agaaataaaa gataatatag tcatgataga tgatggcact
    39601 ggtacgttaa aacaagccgt tttcataggg tataaacaaa tccaaattaa tttaaactgt
    39661 tcacattgtc aaaacttgaa tagaataaaa ttaaatttgt agaattttaa aatggaaatt
    39721 attgaaggaa ttaaaaatag aaagtgttaa tatttagact gaaaattata ttttatacaa
    39781 taaaagttta tttaaataac attgtatttt aatatttatt ttcagtctta ctttattaaa
    39841 attatttttt ggttatgtct ggaaaatatc tgtctactaa tttttctgaa atatttttta
    39901 attggtcagc cattgaagtc agacttaata aggctatcaa taaatcatct ttattttctg
    39961 gatttatact aatattttca aaattagact ctataaagtc cggtatattc tttctttgat
    40021 tttcttttat taacatgaat ttttcgtaac atgaaactaa atctttttca ctgaaattat
    40081 ccttttcata cacaccatgt aagattcttt taatttcttg tattgtaagt gtatttttca
    40141 tagaataaat aattatcatc tgtaaaattt gttctttagt atactttttt cccttaactg
    40201 gtttgattaa tccctctttg ctgtaattat ttatcatggt tttagtaaga attttatcac
    40261 tttcaaatct tttattggca gaaagcttat tgtcaattag agaaataatt tgatccatat
    40321 ataagtcaat agatggaata tcgtttgaag ttatatctgc attatttagt gtttctagta
    40381 ttatagtatt aagttcatta ttcatcatat gttacctcca taatatatat aatacactaa
    40441 attagaaagt aaaacaatat taaggcttgt aaaaaaaaca taaagtggta taattaaata
    40501 tataatatag taatggaaac tatatttgtg aatctgattt ttaaaataca taattgtagg
    40561 aggacgaaat gtatcaattt tttttaaagg gtagagaccc aataagcagt ttaactcatt
    40621 ttataggtgc ttgtttatct cttttagcaa ctataatttt agtttttcaa tctgttacat
    40681 tacaggaaac ttctctcttg atgatagtat ctgtatcggt atttggattt tcactaattg
    40741 ctttatatag cgcaagttcg tattatcatt ttttaaaggg gacaccagaa caagaattgt
    40801 tttttagaaa agtagaccat gcaatgattt acgtattgat tgctggctct tatacaccaa
    40861 tatgtctgaa ttttatggaa aaaaaagaag gcataatatt tgttacagca atatggatag
    40921 ttgcatttat tggtataata ataaaaatat tttggatgga tgcacctaga tggttatcta
    40981 ccagtatata tttacttatg gggtgggcaa ttgtttttga tataaatgca ttcaattcaa
    41041 ttcctaagga ttgccttaga ctattaatta tggaaggtgt atcttactct ataggtgcaa
    41101 ttatttatat aattaaaaag cctaatataa gtcctgagtt tggatttcat gaaatatttc
    41161 atatttttat tatgataggt tctttatttc actttttagc agttttattg tatgttttat
    41221 agttttaaaa gatttgatta attgatacta aaattctaca atgaataaaa tggaactgcc
    41281 ttatatagat tatatcttcg acaataagac agttccttat tataaatatt aaattctact
    41341 tataataaaa ttttgataaa aatagtccat tcaaaatgcc tattccataa ttcctttaat
    41401 gtacttttca tttacgacat atagtttgaa tttatgtact ataattatat cctctaaatt
    41461 ctctgaaaca ctaacaattc tgacacattt gtttctaatt tctacaggta aatctaaatc
    41521 atttgttaaa ttcacatagt catcatattt atacataaaa tcatcccttt ac
//
LOCUS       DAMJYX010000023        41414 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJYX010000023 DAMJYX010000000
VERSION     DAMJYX010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.023"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(163..732)
                     /gene="yfcE"
                     /locus_tag="PC479_001366"
     CDS             complement(163..732)
                     /gene="yfcE"
                     /locus_tag="PC479_001366"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2803919.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(757..1557)
                     /locus_tag="PC479_001367"
     CDS             complement(757..1557)
                     /locus_tag="PC479_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2803920.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            1692..2552
                     /locus_tag="PC479_001368"
     CDS             1692..2552
                     /locus_tag="PC479_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803921.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            2552..3238
                     /locus_tag="PC479_001369"
     CDS             2552..3238
                     /locus_tag="PC479_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2803922.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            3290..3832
                     /locus_tag="PC479_001370"
     CDS             3290..3832
                     /locus_tag="PC479_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803923.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            3911..4378
                     /locus_tag="PC479_001371"
     CDS             3911..4378
                     /locus_tag="PC479_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2803924.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            5087..6223
                     /locus_tag="PC479_001372"
     CDS             5087..6223
                     /locus_tag="PC479_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2803925.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            6282..7064
                     /locus_tag="PC479_001373"
     CDS             6282..7064
                     /locus_tag="PC479_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2803926.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            7083..8093
                     /locus_tag="PC479_001374"
     CDS             7083..8093
                     /locus_tag="PC479_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2803927.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            8112..8909
                     /locus_tag="PC479_001375"
     CDS             8112..8909
                     /locus_tag="PC479_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2803928.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            9007..9852
                     /locus_tag="PC479_001376"
     CDS             9007..9852
                     /locus_tag="PC479_001376"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2803929.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            9939..11114
                     /locus_tag="PC479_001377"
     CDS             9939..11114
                     /locus_tag="PC479_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2803930.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            11309..12211
                     /locus_tag="PC479_001378"
     CDS             11309..12211
                     /locus_tag="PC479_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2803931.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            12225..13229
                     /locus_tag="PC479_001379"
     CDS             12225..13229
                     /locus_tag="PC479_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2803932.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(13373..13600)
                     /gene="acpP"
                     /locus_tag="PC479_001380"
     CDS             complement(13373..13600)
                     /gene="acpP"
                     /locus_tag="PC479_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2803933.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            13772..14197
                     /locus_tag="PC479_001381"
     CDS             13772..14197
                     /locus_tag="PC479_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803934.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            14245..14451
                     /locus_tag="PC479_001382"
     CDS             14245..14451
                     /locus_tag="PC479_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803935.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            14633..14812
                     /locus_tag="PC479_001383"
     CDS             14633..14812
                     /locus_tag="PC479_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803936.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            14846..15046
                     /locus_tag="PC479_001384"
     CDS             14846..15046
                     /locus_tag="PC479_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803937.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            15299..16315
                     /gene="ccpA"
                     /locus_tag="PC479_001385"
     CDS             15299..16315
                     /gene="ccpA"
                     /locus_tag="PC479_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2803938.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(16438..16836)
                     /locus_tag="PC479_001386"
     CDS             complement(16438..16836)
                     /locus_tag="PC479_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803939.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            17048..17959
                     /locus_tag="PC479_001387"
     CDS             17048..17959
                     /locus_tag="PC479_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803940.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            18074..18254
                     /gene="ssrS"
                     /locus_tag="PC479_001388"
     ncRNA           18074..18254
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC479_001388"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            18437..19654
                     /gene="cdeC"
                     /locus_tag="PC479_001389"
     CDS             18437..19654
                     /gene="cdeC"
                     /locus_tag="PC479_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2803941.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(19755..21077)
                     /locus_tag="PC479_001390"
     CDS             complement(19755..21077)
                     /locus_tag="PC479_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2803942.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(21128..21922)
                     /locus_tag="PC479_001391"
     CDS             complement(21128..21922)
                     /locus_tag="PC479_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2803943.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            22032..23477
                     /locus_tag="PC479_001392"
     CDS             22032..23477
                     /locus_tag="PC479_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2803944.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            23506..23715
                     /locus_tag="PC479_001393"
     CDS             23506..23715
                     /locus_tag="PC479_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2803945.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            23735..25129
                     /locus_tag="PC479_001394"
     CDS             23735..25129
                     /locus_tag="PC479_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2803946.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            25369..26121
                     /locus_tag="PC479_001395"
     CDS             25369..26121
                     /locus_tag="PC479_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2803947.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            26147..26560
                     /locus_tag="PC479_001396"
     CDS             26147..26560
                     /locus_tag="PC479_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2803948.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            26564..27307
                     /locus_tag="PC479_001397"
     CDS             26564..27307
                     /locus_tag="PC479_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2803949.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            27307..27777
                     /locus_tag="PC479_001398"
     CDS             27307..27777
                     /locus_tag="PC479_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803950.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            27794..28552
                     /locus_tag="PC479_001399"
     CDS             27794..28552
                     /locus_tag="PC479_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2803951.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            28536..29378
                     /locus_tag="PC479_001400"
     CDS             28536..29378
                     /locus_tag="PC479_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2803952.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(29538..30443)
                     /locus_tag="PC479_001401"
     CDS             complement(29538..30443)
                     /locus_tag="PC479_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2803953.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            30805..31539
                     /locus_tag="PC479_001402"
     CDS             30805..31539
                     /locus_tag="PC479_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803954.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            31750..32931
                     /locus_tag="PC479_001403"
     CDS             31750..32931
                     /locus_tag="PC479_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2803955.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            32915..33487
                     /gene="mobB"
                     /locus_tag="PC479_001404"
     CDS             32915..33487
                     /gene="mobB"
                     /locus_tag="PC479_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2803956.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            33606..33881
                     /locus_tag="PC479_001405"
     CDS             33606..33881
                     /locus_tag="PC479_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2803957.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            34124..35824
                     /locus_tag="PC479_001406"
     CDS             34124..35824
                     /locus_tag="PC479_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2803958.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            36187..37374
                     /locus_tag="PC479_001407"
     CDS             36187..37374
                     /locus_tag="PC479_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803959.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            37434..38597
                     /locus_tag="PC479_001408"
     CDS             37434..38597
                     /locus_tag="PC479_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2803960.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            38748..39551
                     /gene="zupT"
                     /locus_tag="PC479_001409"
     CDS             38748..39551
                     /gene="zupT"
                     /locus_tag="PC479_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2803961.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            39646..40206
                     /locus_tag="PC479_001410"
     CDS             39646..40206
                     /locus_tag="PC479_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2803962.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            40456..41169
                     /gene="rgbR"
                     /locus_tag="PC479_001411"
     CDS             40456..41169
                     /gene="rgbR"
                     /locus_tag="PC479_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2803963.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT        16190 a         4437 c         7363 g        13424 t
ORIGIN      
        1 tttcaatata gcatataata aaacaagtaa accagattct atttaattag aagtttaagg
       61 tattgtactt aaacttctaa ttgagataga aataaaatgg tttacttgtt tttattatga
      121 gtttaataaa ttaaagctta ttttattttt attaaaaatt tactaatcat tgatatttat
      181 tatatttcca gtattgatgt caattaagtt tatatcaagt tcaagctcat cctcatcatc
      241 agtttttatg atatctataa ttgcgacaga atggcttcca tcttttggga tagaagtact
      301 tcctggattt attactatta aattatcatc catgtatagc ttttttacat gagtatgtcc
      361 taaaactaat atgtcaccac ccattttctt tgctttgtca ataatttctt cttctgattc
      421 tatataacca tgattcaata taattctaat ttcaccaaat tggctcatga cataaggact
      481 ctgaatagga tgttctataa ccatctggtc aacatctgca tcacagttcc cttttacaat
      541 cacaatgttt tctaatttat ttaatgtctc aataaatttt ttaggattat atccttctgg
      601 aatatcattt ctaggtccat gatataagac atctccacca tgtaataaaa catcacaatc
      661 agataataca tttaacgctt tttcaaagta aagtaagcta ccatgagtat cactcattat
      721 tcctattttc atattaaaaa tcctcctaag tacagtctat aaaaattttt cagatactct
      781 atgccaaaac tcatagtttg acattctaag taattttatt tttgcatcag atatactaac
      841 ttttatattt tcgatttgtc taaatctgtg ctcaactcca tcaacgacaa tcaatacaga
      901 atcttcaaat ctatattctg gtgcaatctt tataacagat tcatttgaac atattatact
      961 tgatgtaaag cacctatatg cattggtatt tatcggatgt aaaggtgtaa tctgcataag
     1021 ttcaagattt atatcaacaa tactacctcc agcagaatag ttataggctg ttgaacctgt
     1081 ggaagtagaa ataatcatac catctccact aaaattttgt atatgtttat tatcaaggct
     1141 taaatttagg tgtatagtcc tagacttgtc tcctcttata acaacctcat ttacagcaag
     1201 catattagaa ccactagtag ttgtatatac ttccgcatta agaagtgaca tttcttgaat
     1261 aatataatct ttttttgtat aagcttcaat aaagctatca atcttatctg gtaaaatatc
     1321 aggaaaaaac cccagatgac ctgtatttat tcccattatg ggaatttcag gaaaatcgaa
     1381 atctctaact gttcttaaaa aagagccatc accaccaatt gatataataa gctctgtatc
     1441 tggataaata tctgaataca cttcaaagcc agcattgata agttttctag ttaagagttc
     1501 tttagtctca agggacttat ttaattggtt tgtatttata gttataattc ttttcatata
     1561 ttcacctctt catactttgt attataacat agttttataa ttcttaattt ataaatttat
     1621 aaatgacctt tgtagtgaat gtgttaaaat atgtataggt gggtataaaa aaatgcataa
     1681 gaggtgaaga tgtgtttagc ataaggaata tcacaaaaaa attaggaaaa ttcaagttaa
     1741 ataatataaa cttagagtta aaggaagggg atattgttgg gattatagga ccaaatggtt
     1801 cgggaaaaac gactttaata aaaattataa tggggattat tgatgcagat gaaggtgaaa
     1861 ttgaactttg taatgaaact attgaaaatt ctccaataag ctttaaaaac aatattggtt
     1921 ttgtatatga ttctttacaa ttttatccgc atttaaaggt taaggaattt agaaaaatag
     1981 tttctttatt ttataagaat tttgatagag aaaggtttga tgaatacctt aataagtttg
     2041 atatagaaga aaatatgcat attgaaaatt tatctaaagg tcaatctgaa aagcttatgt
     2101 tatcaagtgc acttagtcac aatgctaagt tattgatttt ggatgaacca actgctggga
     2161 tagacccaat tgtaagaact gaaataatgc agtacttaca agattttgtg aaaaatggaa
     2221 gtagtagtgt aataatttct actcataata ctgataattt aattaaaatt gctgattatc
     2281 ttgtttttat aaatagagga aatcagattt ttactgttaa gaaagaatta atagaacaag
     2341 aatataagat aattagggca aataaagcag agttagaagc aattaaagaa agcatagttg
     2401 gtgtgaaaga gtataaatat tataatgagg cacttgttaa agttggagat agcttgaaag
     2461 taaaaagcct attgatagaa attgataagc ataaggtcaa aaatcctact atagaagaac
     2521 taatgtatta ttatgtaaat gaggtgagat gatgttcaat ttaataagaa aagatttaat
     2581 aatagggatt agttcagatg gtatccgtaa tttaaagtac atattgctat tttttgtgtt
     2641 ttattttttc cttaattcaa tttcatatta tacagtaagt attgttgtta gttatttgat
     2701 atttataaat acatttgagt gtgattatga aaatgattca aggattttta taaggagtat
     2761 gccagttagt atagaagata tagtttactc aaaatattta ttaggtgtag gattaataat
     2821 ttctgtaaca ataattgtgt ccttactaag taaattgaca tcactagtgt tctttagaaa
     2881 tatggtttta aatgatgtat ttttttctgt aaatatattt ttagcaattt tgacgatttt
     2941 actgccttta atttttaagt ttggatatgg aaaaatgaaa gtttgtggtt ttatagtaag
     3001 tattttaatt tactttgtat atggaagtct tttgaagatg attagtatga ttgtatatca
     3061 agtaaagcat tttaattata gtaaagttgg aggagtatat ctttcaaatt atgtaactga
     3121 tatagctaat actaagtata taaatttata tagtatgaca tttttaacaa taataatttt
     3181 tataatatca atgtattttt ctattaagat ttcaaaaaag aataagttta attattaaaa
     3241 ttttgattaa tattatagca caatagcaca aaatataagg agactaaaaa tgaagaagtt
     3301 tataaaagtt tcatttttta ttttggggat ttttacacta ttcatagcta taaataaatt
     3361 agtatataga gatatggtaa gtgtcaaaga ttatgaaata aatcatttag aggaaattgt
     3421 ttttgatttt gatagcagga gaagtagaga tgttggtact attgtaagat ttaaaataag
     3481 caataatagt aaatatagtt ataaattaaa cagtgctaaa atgaagtttg aaaattgtgt
     3541 ggaaaataaa aagggaatga ataagtgttc tgcatatgta aatgtagatt tatttaatga
     3601 aaaaaattct ataactgaaa gaactctgaa atatggaata aaaccaaata atcaaggtta
     3661 tgttgatttt gtaattccaa aaggactaaa ttttgattat aggtacttta atgaatctgg
     3721 tatgagtgta gagtataaag gagaatatat tgtcaatatt ccaatggcaa aaggacttta
     3781 tctagtagtt ggaaaaaata atgatacatg gaatgctaat attatggaat aatttgaaaa
     3841 ataaatcaat tatagatata attaagaata atatgaaatt tataaaagtt cagactagga
     3901 ggggtattta atgtcaataa gtaatgtaag agagtatttt aaacaatttg gcaaggaaga
     3961 cagtatatta gaatttgaac aatctagtgc tactgtggaa cttgcagctg aagcagctgg
     4021 ggttattcca gcaagaatag ctaagacact ctcatttaaa ataggagatg atgcaatttt
     4081 aattgtgaca gctggtgatg ctaagattga taataagaag tataaggctg aatttaattg
     4141 taaggctaaa atgttgacac cagaagaagt cttggagttt actggacatg ctattggtgg
     4201 agtctgtcca tttggtttaa aaaattctat aaaagtctat ttagatgatt caatgaaaag
     4261 atttgataca gtatttcctg catgtggcag tagtaactct gcaatagaac ttacatgtga
     4321 agaaatggag aagttttcta agtgtgaaaa atgggtagat gtttgtaaga attggtaaaa
     4381 aaatatagta aactatagta ggaattagta aaaaactacc tctgtataca atctatacag
     4441 aggtagtttt tttgtgtaaa tatttataaa tctaagagga aaatctaatt taaactaata
     4501 atccaaaata aagttaaaaa attctgattt tttttcgaaa caataaaaaa atgacaaaaa
     4561 agtggaaaaa atgtaataaa tattgttaaa aaaataatta gaaattattg aaaaattatc
     4621 ctttgagtga tactattata gatgtaagat atttaaaaat aaaaaatagt cagataaaaa
     4681 tatgattatt tagctctatt aaaataattt ttatacgata aaaatcttct gtacatggct
     4741 aaaaaacaga tgtacagaca atatttaatg tttaatatac cttaatataa atatcgtaaa
     4801 caaattattt tatctctaac cagtgtgaaa gaaatcttat tctaacttga aatttcacaa
     4861 aattccaaaa agcgagattt tttttaagta tagttgattt taaattaaca atttatgatt
     4921 aaattaatgt cataattggt tagtataatc acatataaat atagagcgta gtttgattaa
     4981 ggttataaat ttctcgacgt atattcaaat accttattat agatgttgcc gagattttaa
     5041 ttaataaatg aaaataaaat atttataaaa tactgaggag gctattatgg atttaaattc
     5101 taaaaaatat cagatgctta aagagctata tgtaagcttc gctgaaaatg aagttaaacc
     5161 tttagcaaca gaacttgatg aagaagaaag atttccttat gaaacagtgg aaaaaatggc
     5221 aaaagcagga atgatgggta taccatatcc aaaagaatat ggtggagaag gtggagacac
     5281 tgtaggatat ataatggcag ttgaagaatt gtctagagtt tgtggtacta caggagttat
     5341 attatcagct catacatctc ttggctcatg gcctatatat caatatggta atgaagaaca
     5401 aaaacaaaaa ttcttaagac cattagcaag tggagaaaaa ttaggagcat ttggtcttac
     5461 tgagcctaat gctggtacag atgcgtctgg ccaacaaaca actgctgttt tagacgggga
     5521 tgaatacata cttaatggct caaaaatatt tataacaaat gcaatagctg gtgacatata
     5581 tgtagtaatg gcaatgactg ataaatctaa ggggaacaaa ggaatatcag catttatagt
     5641 tgaaaaagga actcctgggt ttagctttgg agttaaagaa aagaaaatgg gtataagagg
     5701 ttcagctacg agtgaattaa tatttgagga ttgcagaata cctaaagaaa atttacttgg
     5761 aaaagaaggt caaggattta agatagcaat gtctactctt gatggtggta gaattggtat
     5821 agctgcacaa gctttaggtt tagcacaagg tgctcttgat gaaactgtta aatatgtaaa
     5881 agaaagagta caatttggta gaccattatc aaaattccaa aatacacaat tccaattagc
     5941 tgatatggaa gttaaggtac aagcggctag acaccttgta tatcaagcag ctataaataa
     6001 agacttagga aaaccttatg gagtagaagc agcaatggca aaattatttg cagctgaaac
     6061 agctatggaa gttactacaa aagctgtaca acttcatgga ggatatggat acactcgtga
     6121 ctatccagta gaaagaatga tgagagatgc taagataact gaaatatatg aaggaactag
     6181 tgaagttcaa agaatggtta tttcaggaaa attattaaaa tagataaata ttaaaatata
     6241 cacaataggt cgatattaaa atagatatgg aggaaggatt tatgaatata gtcgtttgta
     6301 taaaacaagt tccagataca acagaagtta aactagatcc taatacaggt actttaatta
     6361 gagatggagt accaagtata ataaaccctg atgataaagc aggtttagaa gaagctataa
     6421 aattaaaaga agaaatgggt gctcatgtaa ctgttataac aatgggacct cctcaagcag
     6481 atatggcttt aaaagaagct ttagcaatgg gtgcagatag aggtatatta ttaacagata
     6541 gagcatttgc aggtgctgat acttgggcaa cttcatcagc attagcagga gcattaaaaa
     6601 atatagattt tgatattata atagctggaa gacaggcgat agatggagat actgcacaag
     6661 ttggacctca aatagctgaa catttaaatc ttccatcaat aacatatgct gaagaaataa
     6721 aaactgaagg tgaatatgta ttagtaaaaa gacaatttga agattgttgc catgacttaa
     6781 aagttaaaat gccatgcctt ataacaactc ttaaagatat gaacacacca agatacatga
     6841 aagttggaag aatatatgat gctttcgaaa atgatgtagt agaaacatgg actgtaaaag
     6901 atatagaagt tgacccttct aatttaggtc ttaaaggttc tccaactagt gtatttaaat
     6961 catttacaaa atcagttaaa ccagctggta caatatacaa tgaagatgcg aaaacatcag
     7021 ctggaattat catagataaa ttaaaagaga agtatatcat ataatttaag gggggataaa
     7081 gaatgggtaa cgttttagta gtaatagaac aaagagaaaa tgtaattcaa actgtttctt
     7141 tagaattact aggaaaggct acagaaatag caaaagatta tgatacaaaa gtttctgcat
     7201 tacttttagg tagtaaggta gaaggtttaa tagatacatt agcacactat ggtgcagatg
     7261 aggtaatagt agtagatgat gaagctttag cagtgtatac aactgaacca tatacaaaag
     7321 cagcttatga agcaataaaa gcagctgacc ctatagttgt attatttggt gcaacttcaa
     7381 taggtagaga tttagcacct agagtttctg ctagaataca tacaggtctt actgctgact
     7441 gtacaggtct tgcagtagct gaagatacaa aattattatt aatgacaaga cctgcctttg
     7501 gtggaaatat aatggcaaca atagtttgta aagatttcag acctcaaatg tctacagtta
     7561 gaccaggggt tatgaagaaa aatgaacctg atgaaactaa agaagctgta attaaccgtt
     7621 tcaaggtaga atttaatgat gctgataaat tagttcaagt tgtacaagta ataaaagaag
     7681 ctaaaaaaca agttaaaata gaagatgcta agatattagt ttctgctgga cgtggaatgg
     7741 gtggaaaaga aaacttagac atactttatg aattagctga aattataggt ggagaagttt
     7801 ctggttctcg tgccactata gatgcaggtt ggttagataa agcaagacaa gttggtcaaa
     7861 ctggtaaaac tgtaagacca gacctttata tagcatgtgg tatatctgga gcaatacaac
     7921 atatagctgg tatggaagat gctgagttta tagttgctat aaataaaaat ccagaagctc
     7981 caatatttaa atatgctgat gttggtatag ttggagatgt tcataaagtg cttccagaac
     8041 ttatcagtca gttaagtgtt gcaaaagaaa aaggtgaagt tttagctaac taatttcttg
     8101 gggagggtat tatgagtaca agtgatgtta aagtttatga gaatgtagct gttgaagtag
     8161 atggaaatat atgtacagtg aaaatgaata gacctaaagc ccttaatgca ataaattcaa
     8221 agactttaga agaactttat gaagtatttg tagatattaa taatgatgaa actattgatg
     8281 ttgtaatatt gacaggggaa ggaaaggcat ttgtagctgg agcagatatt gcatacatga
     8341 aagatttaga tgctgtagct gctaaagatt ttagtatctt aggagcaaaa gcttttggag
     8401 aaatagaaaa tagtaaaaaa gtagtgatag ctgctgtaaa cggatttgct ttaggtggag
     8461 gatgtgaact tgcaatggca tgtgatataa gaattgcatc tgctaaagct aaatttggtc
     8521 agccagaagt aactcttgga ataactccag gatatggagg aactcaaaga cttacaagat
     8581 tggttggaat ggcaaaagca aaagaattaa tctttacagg tcaagttata aaagctgatg
     8641 aagctgaaaa aatagggcta gtaaatagag tcgttgagcc agacatttta atagaagaag
     8701 ttgagaaatt agctaagata atagctaaaa atgctcagct tgcagttaga tactctaaag
     8761 aagcaataca acttggtgct caaactgata taaatactgg aatagatata gaatctaatt
     8821 tatttggtct ttgtttttca actaaagacc aaaaagaagg aatgtcagct ttcgttgaaa
     8881 agagagaagc taactttata aaagggtaaa aatctaatta aaaatatatt tgctgttaac
     8941 tagattaaat agtattttaa tctagttaac agaatataaa tactaaaata acaggaggtt
     9001 ataactatga aattagctgt aataggtagt ggaactatgg gaagtggtat tgtacaaact
     9061 tttgcaagtt gtggacatga tgtatgttta aagagtagaa ctcaaggtgc tatagataaa
     9121 tgtttagctt tattagataa aaatttaact aagttagtta ctaagggaaa aatggatgaa
     9181 gctacaaaag cagaaatatt aagtcatgtt agttcaacta ctaactatga agatttaaaa
     9241 gatatggatt taataataga agcatctgta gaagacatga atataaagaa agatgttttc
     9301 aagttactag atgaattatg taaagaagat actatcttgg caacaaatac ttcatcatta
     9361 tctataacag aaatagcttc ttctactaag agaccagata aagttatagg aatgcatttc
     9421 tttaatccag ttcctatgat gaaattagtt gaagttataa gtggtcagtt aacatcaaaa
     9481 gttacttttg atacagtatt tgaattatct aagagtatca ataaagtacc agtagatgta
     9541 tctgaatctc ctggatttgt agtaaataga atacttatac ctatgataaa tgaagctgtt
     9601 ggtatatatg cagatggtgt tgcaagtaaa gaagaaatag atgaagctat gaaattagga
     9661 gcaaaccatc caatgggacc actagcatta ggtgatttaa tcggattaga tgttgtttta
     9721 gctataatga acgttttata tactgaattt ggagatacta aatatagacc tcatccactt
     9781 ttagctaaaa tggttagagc taatcaatta ggaagaaaaa ctaagatagg attctatgat
     9841 tataataaat aagaatttgg gaattaaaag tttaaataaa attgtttaaa aaacaatttc
     9901 ggatatatga aaaatctaat ttaatggggg taatgaatat gagagaagta gtaattgcca
     9961 gtgcagctag aacagcagta ggaagttttg gaggagcatt taaatcagtt tcagcggtag
    10021 agttaggggt aacagcagct aaagaagcta taaaaagagc taacataact ccagatatga
    10081 tagatgaatc tcttttaggg ggagtactta cagcaggtct tggacaaaat atagcaagac
    10141 aaatagcatt aggagcagga ataccagtag aaaaaccagc tatgactata aatatagttt
    10201 gtggttctgg attaagatct gtttcaatgg catctcaact tatagcatta ggtgatgctg
    10261 atataatgtt agttggtgga gctgaaaaca tgagtatgtc tccttattta gtaccaagtg
    10321 cgagatatgg tgcaagaatg ggtgatgctg cttttgttga ttcaatgata aaagatggat
    10381 tatcagacat atttaataac tatcacatgg gtattactgc tgaaaacata gcagagcaat
    10441 ggaatataac tagagaagaa caagatgaat tagctcttgc aagtcaaaat aaagctgaaa
    10501 aagctcaagc tgaaggaaaa tttgatgaag aaatagttcc tgttgttata aaaggaagaa
    10561 aaggtgacac tgtagtagat aaagatgaat atattaagcc tggcactaca atggagaaac
    10621 ttgctaagtt aagacctgca tttaaaaaag atggaacagt tactgctggt aatgcatcag
    10681 gaataaatga tggtgctgct atgctagtag taatggctaa agaaaaagct gaagaactag
    10741 gaatagagcc tcttgcaact atagtttctt atggaacagc tggtgttgac cctaaaataa
    10801 tgggatatgg accagttcca gcaactaaaa aagctttaga agctgctaat atgactattg
    10861 aagatataga tttagttgaa gctaatgagg catttgctgc ccaatctgta gctgtaataa
    10921 gagacttaaa tatagatatg aataaagtta atgttaatgg tggagcaata gctataggac
    10981 atccaatagg atgctcagga gcaagaatac ttactacact tttatatgaa atgaagagaa
    11041 gagatgctaa aactggtctt gctacacttt gtataggtgg aggaatggga actactttaa
    11101 tagttaagag atagttttag attataatat ttacaaatta aatatttaat tatgaattta
    11161 acctgtaaga ataactattt gttattctta caggttttta tagttaaaaa taattattat
    11221 ctattaaaca aaaatagtgt aaaatatata atatcatgat tttgaagata taaaaagtag
    11281 gaagatataa aagaaagtag gtaaaagttt gtttaaaaaa gagaatcaaa gatataatct
    11341 tatatcgtat acaaatgaag aagaaatgac tttaaaagaa gttttattgg acaagctaaa
    11401 tttctcagtt aggtcattat caaaaatgaa gagggaaaag agtgttttag taaatggagt
    11461 atataaaaaa ccgagtttaa aggtgtatag tggtgattta atagaagtaa aaatagacga
    11521 ggaaaaagca aattttgaac ctcaagattt aaatttacag ataatttatg atgattttga
    11581 tataattatg gtgaataaac ctccgtttat ggtagttcac ccaacaaaaa gccactatga
    11641 taaaacaata gcaaatggta ttagttacta tatagataat caaaaagaaa acgtaaaaat
    11701 aagatttgta aataggttag atatgaatac ttcagggtta gttatagtag caaaaaatgc
    11761 ttatgcacat catactttgt ctacagcaat gagtgaaaat aaagttgaaa aaaagtatat
    11821 aacagtagtt gatggaatta taaaagaaaa tgagggaact atagatgagc caatttatag
    11881 acctacagaa gattcaataa agaggataat agatgaaaga ggacagtcat ctgttactca
    11941 ctataaagta atagaaagac tagaaaatgc aactgtatta gaagtaagct tagaaactgg
    12001 tagaacacat caaataagag tacatatggc tcatataggt catggaataa taggtgatga
    12061 gttgtatggt tatgtagatg aagagctaat aaataggcaa gcacttcatg catataagct
    12121 agagtttgaa caaccaagaa caaaagaaaa attgaaattt aaagcagata taccagagga
    12181 tatgaaagag ttaatatcga aattaagata atggggtgat atatatgata atacataaat
    12241 ttataataca tgttttggac aaaaatagtg atgttccaat attaaatgac tttgaaggca
    12301 aagttaatca agaagtggat ggttttttcc agaaagctat aaaaagaatt gcaaaagatg
    12361 aggatttaag aaaaggtgtt tttaaagatt ataatgacaa tttaattaaa aactgttgtg
    12421 aacaaataat atatgatgaa agtacttttt tgaaaaattc taaagaaatt gcatcttact
    12481 tgtttgacgt tatgaaaatt aatgctgcac tagaatcttg tgatttggca atttgtttat
    12541 acactattaa agatgaaaag agtgtagcaa tactgaaatt agattataaa aagttgtaca
    12601 ctcattcaat tgaatttgtg gatgataaat ttaatattca aatggtttca aatgaaattg
    12661 gtatcccaga gactttaaga caaaagcaag gagctttgat tagtctaagt ggtataaatg
    12721 atgagtttca tctaaggctt ttagataagg atgctgaaaa agaagggtca gaatctaagt
    12781 ttgtaacaga atttttaaat gctaaaaaaa tagatgatga taagtacaag accaaggtat
    12841 ttaaaaatac agctgaaaat tggataacca atgctcttag taatgatata aagcaagctg
    12901 aagatgtaag aagtatatta aattatacac tgaaggaaaa acatgaagtt gatataaatg
    12961 actttgttga taattcaatt aaagatgatg agttaaagga tagctttaaa gagcatatgg
    13021 aagaaaaagg tcttgacgaa agttttagta tagataaaaa atgggttgag aaaaagctta
    13081 aaaaaagaag tataaaaacg gataatggtt ttgacataaa agggaatcta actgattttg
    13141 aagacccaat gaagtatact gtaaaacaaa atcaagatgg aactatagat ataataataa
    13201 agaatgttac gttttatgaa gaaaagtaat ttatatactt agtatataaa ttaaatataa
    13261 gagcgtgtat tttatatatt gtaaataaat gtaatctagt ctgtgcgaac tctaatataa
    13321 tttattatgt agaagtttac acagactatt tatatttgta gacaaaacga tactatttgt
    13381 tttcttcgat atatttacat atatcaccaa tacatttaaa gttctcagct tcctcgtctg
    13441 gtatttctat tccaaattca tcttcaaggg ccattataat ttcaacagca tctaatgaat
    13501 cagcttctaa gtcgtccatt aaagatgctt ccatagttat ctcatctaaa ttgtctaccc
    13561 ctaattgttc tgctattatt tcttttattt tctcaaacat tttaagcctc ctaaagcagt
    13621 tttaattaat tttaatgtta gtttaatata atatcaagaa tttttcaata tggaatctat
    13681 aaaaaataat aaaattttat aaatgtagta ataaagtatt atttgatgaa taaactttat
    13741 tgataacaaa tataacttag gaggaaatgc tatgaaatat gtaggactat tactaacttc
    13801 agtaggaatg tttttactta tagcagtaaa tttttattat aattcaataa ctttagatat
    13861 gcaacgtata gaggattatg taatggagac caatttaatc cttgaagatg tagctgaaaa
    13921 ggagagttat gtatctaatg aaaaagaaga ctacatatca aggttaatgc atgtaaagaa
    13981 aggaatagaa aattcaaaaa catccttctt gatagaaaga tataaggagt acaaaataaa
    14041 atcaatagaa agtttaatct atactatatc agaggagaaa aaagattatc tggatgaagt
    14101 tgatagatac aataaacttg gtgaaaaaga aataaataaa ctaatcaata aaaacttttt
    14161 agaagtaact tatctgtcta ttactacata tatttaatta aagagacaaa acttagtctc
    14221 ttaatgattc aaggaggatt agttatgaca aaattagaag acaatatgga acgtttcttt
    14281 ggaggatgcg aaggattttt tgggaataaa atgttgataa taatagttat agtattctta
    14341 ctattatgca cagacatttt agaagactta ttatgcgatg acaatatatg gatatgggtt
    14401 atattaatag tattgttatt atttaacttc gatgatggtt gttgttgcta aaaaatattg
    14461 attattaaat aggtaaaacc agtaacttat acaagttatg gttttacctt ttttagttca
    14521 aattaaaaaa tttaaaaaaa tagtaaaaat acattagcaa aatggaaacc aaatgcatag
    14581 tatatgttag gagcgaaaag ctcaacaaaa tatcaacagg aggtaacgta taatgttaga
    14641 tagattcttt ggagaagaag gatgttttgg tggaggagga tggtggataa tagtactatt
    14701 cttcctattc ttagcttttg gagatagctg ggcagaattt gacataatgg catggatacc
    14761 attcctaata gttttattaa tactttgctg ctgtggtgga tgcttagaat aagaacacag
    14821 ttattaaaaa aaggagggct ttgttatgtt agatagattt tttggagaag aaggatgctt
    14881 tggtggagga ggatggtgga taatagtact attcttccta ttcttagctt ttgaagattg
    14941 ctggagagac attgacataa tgtcatggat acctttctta atattattgc taataacttg
    15001 ttcatgtggt ggattctttg atggtgaagt aggttgcggc tgctaatact taaatcatta
    15061 gaataaaaaa atgctggtat tttataccag cattttttta ttttattttt taatatgatt
    15121 taaattaaga atgaatatta ttaaatttgt tgaatatagg gtataatata aaatataatg
    15181 tatttaaatg tacttgcttt tatggtaaaa taaaaactta tataaatgta gttaaaatag
    15241 gaaccttcaa aaatttggtt cagttttaga taaatttagt aaaataagga ggatgaagat
    15301 gaaaggcaat ataacgataa aagatgttgc taaacaagca ggagtgtcaa tatctactgt
    15361 atctagagtt ataaatgatt caaaacctgt aactgatgaa gtcaaacaaa aagttttaga
    15421 ggttataaaa gagactggat atataccaaa tccacttgct agaagcttag taacaaagaa
    15481 gagtcaatta ataggggtaa tagttccaga agtttcagat tcttttgtta atgaggtgtt
    15541 aaatgggata gaagaggttg ctaaaatgta tgactatgat attcttttag cgaatacata
    15601 ctctgataag gaacaagaac ttaagagtat aaatctattg agagcaaaac aagtggaagg
    15661 tatagttatg atttcatgga tagttgaaca agaacatatc aactatatac aaaattgtgg
    15721 aataccagcg acatatataa gtaaaactgc tagaaattat gatatatata cagtaagtac
    15781 tagcaacgaa gaagctactt ttgatatgac agagcatctt ataaagaaag gtcatgaaaa
    15841 gatagctttt ataatgacga gtaaagatga tactgtttta gaaatggaaa gacttgctgg
    15901 ttatgagaaa gcactttcaa ataacaatat agaattagac aagagtttga ttaagtatgg
    15961 tggaactgat tatgagagtg gatacaatag tatgaaagaa ctattagatg atggaataat
    16021 acctcatgcg gcttttgtaa caggtgatga ggctgccata ggtgctataa atgctatatg
    16081 tgatgctgga tataaggttc cagaagacat atctgttgca ggatttaatg atgttaagat
    16141 agctagaatg tatagaccta aacttactac agtatatcaa cctctatacg atatgggagc
    16201 agtagcaata agaatggtta taaagttaat aaataaggaa ttaattgaga ataagaaaat
    16261 agaattacct tatagaattg ttgatagaga aagtgttaca gaaagaaaaa aataaaacta
    16321 ttaaaatcaa tcaggtagtg cctaaggcac tacctgattt tttatgtcaa taatatcaaa
    16381 tttaataata gattaactaa aaataattat ctaattactt aaatagttta tttttatcta
    16441 ttcatgaggg ttagactttt tagttttctt aaataaactt ttagattttt tttgttgact
    16501 acctggtgta ttttttggtg caggtgaatt tgacttacct ttagtaggtt tagccttctt
    16561 agttttagct ggtttagtat ctctttggtt ttctgcatct gagatttttt ccattatatt
    16621 atccagtttt ttgcgttgat tttcatctaa aaatgctttg aattttgata ctacttcatc
    16681 tctgccatct aggttcttac ctatttttat aagttcctcc ataagttcat tttctgattt
    16741 tcctttgtaa ctatcagcca tcttttctat tttatccttg tcaatatttt tttgtttagc
    16801 cattttttct aatgactctg tatctaattt tttcataaaa aaaccccttt ctataaatat
    16861 atattgaatt atattcatac tatttatata tagaaataat attgaagtgg tgaattgtga
    16921 gtgatatata atataaataa aaattcttat agataatata tatgcactga gctttaaaaa
    16981 tggaacatta aaatatgaaa aacatacttt aatatagggc aattatataa tataaggggg
    17041 aattgtcttg aataacttac tatttttgat tggagtgatt gcattgagta atggtaatat
    17101 ctctatattt gatgataaaa ataaatcaaa aggaaataat agagttagaa aaaacaaaaa
    17161 aaatgcaaat gataaacttt cacagtctac acatggcaat ttaaggtttg atgcaaatga
    17221 tataaaaaga ggtttaaggc tcatggattt aagtgaagaa gatttagaaa tgggaatgga
    17281 aataataaca agaactaaaa aatacatgtc aagagatgaa agaaaaatac taataaaaat
    17341 agaaagtgtt ttagatttag ttagaggaat aaaaaaatta aataatatag atatggtaga
    17401 tacagaagat gaaacggatt ttttcagaaa aatggatagt gatgataaaa agaatatgat
    17461 gataaaggaa attatagatg tattcccaga aaaaagaaaa aattcagttg aaaaagctat
    17521 aggaatgaaa aagaagatag atttatttgc agaattattt ttaccagatg actttggtga
    17581 aggtggattt agtttgagct ctttggcaaa tataaacaat cttggtagta tgaataatct
    17641 caaattgtta ggaagtcttt taagaggtga tgatagtaat aatgatgatg aagacaataa
    17701 tgatgagtta aattatgaag acgaagaatt agattgtgaa gacaaagaat tggatgatga
    17761 ttatgatgag ttaacagacg aaggtgaaat ggaggataat gaagatgaat tagtagaaga
    17821 taagaacaca ggctatgaaa atatagaaaa tgatgaagat atagtatatg atgaggaact
    17881 atacaacatt aatgagcaag aatccgaata caattataat gaaaaaaata ctaaaactag
    17941 agctaagaaa aaacgataat taactaaata cataaagaaa aaaggaagaa aaagtaatga
    18001 tagaataaga ttatctcaat tactatataa aaccacttta aaaaattcaa aatttattat
    18061 ataataataa tatgaaatcc tgaaacatac gataaggctt ttttaaattc aacctacaaa
    18121 tgaaatacgg gagaccgtgt ctggtaaatt ataacatgcc tagttagttt ggacgttaaa
    18181 ggttactggc aagtcaccca cctgggagtg cctagggtgt gaatttactt agccaccggt
    18241 gtttcgggac aaaaaaagtg ctactatttc aaatagtagt acttttttta ttaatatgca
    18301 tttttctact aaatacgcct aattattgat aaattcaaaa agcaagtaac aattaagatt
    18361 ataaaagcat agtattaatt aggcttcaaa aagccaaaga cttaataaga atcgcttaca
    18421 gggagggcaa tgaaaaatgc aagattataa aaaaaataaa agaagaatga tgaatcagcc
    18481 aatgtctaca atgaatgaag aagaagtgta tacagatgaa ataaattcag aagacatgag
    18541 aggttttaaa aaatcacacc atcataatgg atgtaatact gataataagt gtgagtgcca
    18601 tgatgattgc aatccatgca acccatgtaa tccatgtaaa cctaacccat gcaatccatg
    18661 caaacctaat ccatgtgatg acaattgtgg atgccatgac aattgtaaat gtgattgtga
    18721 accatgtgaa atggattcag atgaatgttt tgaaaacaaa tgtggaccag aatgctgtaa
    18781 tcctatatct ccaagaaact tctctgtatc aaatgcagtg ccatttgcaa tagaggctaa
    18841 tagaatattt gatactatgc aattccaaac atttacagat gcaacaggac caaatggaga
    18901 gccattaact tttgaaacag aagtagtaga agtatttggt tcagttccaa gtgcaggtca
    18961 agcaagtgta actatagaaa aaatatgctt aagtaatgat ggaatcgtta tagacacagg
    19021 aatgacaact ttagaagatt tcgatttaga cccattagga gatatagtag gaagaaactg
    19081 tgaaacaact tttgaatttg cagtttgtgg agaaagaaac gctgagtgct gtagacaagg
    19141 aaaaggcaaa tcagtagctt ataaacaaag aggattaact gtagcagttc gtaatttagt
    19201 actagagcta agaggtagat gtggatgtac agagttcgtt gcattagctt tcccagcagt
    19261 tagagcagga ggtggatgta agagaagagt tgattatgta gaatttactt ttaacacact
    19321 ttcagcacca atatgcttgc cagctgacgg aagagctgtt actttaagac aagaatatca
    19381 aactaactta actgtagatt gtataggaaa atctatatta aaattagaat gcaacgaatg
    19441 ttgtgaacct ttctatgaat taattatacc aaatgatata gatttagtac tttgcttaca
    19501 agaaacagtt agcacattaa taagtgaaca aatagtagtt ttagcatcac caaatccaat
    19561 ccaaccaaga cttgttgata ctttctctaa agtatgtgat ttttcgcaat gtggacctaa
    19621 tcatggaagt ggaaagccaa gttgccacag atagataggt aagatatata gacagtttaa
    19681 attataaata aaaatcatct aaggaaggct accaaagtgt ataatttaca ttggtagcct
    19741 tcacgttttt ataactagcg tatagatttt agtgatttaa aaagcactat agtatgtaat
    19801 agacaagtaa agattgatac tatcgtcatt gtgtatatgt atgcataaat attaagtcct
    19861 ggaattggaa gaaatacata taaagctatt agttgaatta acatgccagt aatagtattt
    19921 atacttgaag ctaattcttt tcttattgaa tgtaaaatgc cagatagagt ttgatttagg
    19981 gacataaata gtggaactaa aaacatagct tttagatatt ctcctgcaag tgtattttta
    20041 aatactaata tacataaatc ttttccaaag aaataaaaaa atattgatga tagtatgcct
    20101 acaaataaag taagaagtat ggagtatttg atttttttaa ttacactctt acgctttctt
    20161 agagccattt cttgtgaaat agttggtatt aaatttacaa taagcgctga accaacagta
    20221 aaaggtaaaa atataaaagg cattaccatc ccactgacaa taccatacat acttaaagat
    20281 tgttgataag tcattcctga taatgctaat cttgatggta tcatcataga acttatagat
    20341 tgcaaaagta tgtttgacat tctattacaa gttatgggaa tagacatttt taatgtttcc
    20401 atagaagaat tataaaaatc ttttatgctt ataatatacc tattgtcaaa tgatgaatct
    20461 cgccataagc aaataagcat aaatataata ttactcaact caccgattga tatacctaat
    20521 agagctatat aacaattcat agatttatta tttatgtaca taactagtaa aaaaacaaat
    20581 aatattttac caatttgttc aatgatttgt ccaatagcag ggatttttac attctttatg
    20641 ccatagtaat atcctcttag tacgtttgaa agagttatta taactatagc aggacaaaca
    20701 gctagtataa atagatttaa tttagcatct tttaaaaatt taagtgataa aaaagaacta
    20761 ttgaaagatg caactataga tataattagt gctacgaaaa atgatatata taaagttgat
    20821 ataaataaag cattagtatt gtgtctatcg tttaaagctt ttctctttgc aactagacaa
    20881 ctaagtgcag taggtatacc tgttgttgta acagctaagc aaatcatcaa aaagttaaaa
    20941 atcatatgat agatacctaa acctgtttca cctagtgctc ttgataagaa tattttatat
    21001 aaaaaaccaa atattcttac aataaaattg gatataaata aaataactgt tgatagtact
    21061 aaataaggta ctttcaaaaa aatcacctcc ttatctttta aatatattca caaggaggtg
    21121 taaaaattta taattttata aattctggtt tcaatttatt aaatttagat acatatttaa
    21181 atcccatatt ttttagtaat gaatatatgt agtcaaactc acatgcaact tgacttatat
    21241 gatgtgaatc tgaacctgtg gttatgattt cccctcccaa gtctttatac atttgaagta
    21301 tatacttaga tggggttagg tctggaagat tatatctata acaagaggta tttatctcga
    21361 tacctttgcc atcataaata gcttgtttaa gagtttcttc aattatatca gaaaatagtc
    21421 tatcatctaa aatagtgtca taaggtgcat accttttaat caaatctaaa tgacctaaga
    21481 cagagtaatc tttataattc ttaacaatat tatataaata aagatagtaa ttttcataaa
    21541 cttcatgttg agttttatct ttaaaataat taccaaggta taagtccatt gtatctatag
    21601 catggataga acatattata aagtccaatg gatattgatg tgcttccttt ttgtatgtat
    21661 ccattaattg agtttgaact ccaaattcaa ttcctttttt tattgatatt ttatctttat
    21721 atttgctttg aagagtctct aaactcttaa aataatcttc gtagacaatt gaaaaagaat
    21781 catcagcata tacatcatag tcaacatggt ctgtgaaaca aatttcctcc aaaccgagtt
    21841 caatagattt ttttaccatt tcttccattg tagattttcc gtctgtagag aaacttgagt
    21901 gcatgtgata gtcgtaaaac ataaatattt ccccttttta taaaaatttt ggtattatat
    21961 atacatacta aataatatca aatatacata aaagcatcaa taaaatataa atacaatgag
    22021 cggtgataaa tttggaaaaa tacagaatag atttaaatga gaaaaaatca actaaatata
    22081 tacagatatt taatcatata aaagaattga taataaataa agagttagta gaacatgaaa
    22141 aattgcctcc aataagaaaa ctatcaaatt ttttacaagt aaataacact actatagtta
    22201 aggtatacga gcttttagaa aatgaaggat atgtatataa aattataggg agtggtactt
    22261 ttgtatcaag tttaaattct tataaaagcg gtatagataa caggaaaaat aagattattg
    22321 tgcaaagaga agatttaatt cattttgaca atgggaatcc atctaatgat atgtttccta
    22381 tcgattcatt taaaaatgct gtaaatatgg cattatctaa agatggtagt tcaatatttg
    22441 aatatgatga aggtcttggc tctgaagaat taagagaaaa aatggtagag tatctaagtg
    22501 atggaaatat aaaaactact aaggaaaata ttcaaataat atcaggtgct caacaaggta
    22561 tagatatagt ttgtaaagga cttataagtt attcagatgt tgtttttatg gaagaacctt
    22621 cttacaatgg agcaattgaa gtgtttaaaa gtagaggtgc aaaaataata tctataccta
    22681 tgttagatga tggaattgat ataggtattt taaaattaaa gttggaaaaa ataaaaccaa
    22741 gacttatata cattatgcct aattttcaaa atccaactgg aatatcttac tcaacatata
    22801 aaaaaaagaa attgatagaa ttggcagaag aacatgattt ttatataata gaagatgatt
    22861 ttataagtga ttttgttttt gaatcaaagg acaatagaac aattagaagt tatgatgata
    22921 aaaatagagt agtatatata aaaagctttt caaaaatact tatgccaggt cttaggatag
    22981 gaattgtaga aatgccaaat gagttgttaa aaaaagtttt atgggctaaa tattcatcag
    23041 atatatcaac accaggttta attcaaaaat caatgtatta ctatatgaat aattttgatt
    23101 ggaagaatta tttgagtcat atagaaaaga tatatactga taagtataaa ttggcaaaaa
    23161 acttgattaa tgacaaattg agtggtaaat taaaagttag aaaatcttgt ggaggaataa
    23221 actttttttt agaattacca agaggatata catctcaagc atttacaaat tttatgttaa
    23281 acaaaggagt gtcaatgctt ccagggactt atttttttga caatctagtg gatgatagat
    23341 tctttaggat aaatatagct cgcccaagca tggaagaact agaaaaggga atatctataa
    23401 taagtgataa tatagatgag tttttttgtg aatataaaaa caagttagat ataaaaagca
    23461 ataaattatt ttattaaata aatagaggtt ttttaggagg aaaaaatgtt tgacaagaag
    23521 aaattagata ggattaatga attagcaaag aaaaataaag agggaatcct ttctgctgat
    23581 gaaatcaaag aaagagaaat tttaaggaaa gaatatttag aaaactttag agcccacttt
    23641 agatcaagac tagatagtgt caaagtagtt tcaccagaag aatatgagca atacatgaaa
    23701 aacaataaaa attagaactt agtaggagga aaatatgaac ttaaagtacg aatttaagaa
    23761 tgcttgggat tttgaaagaa aagaaaatac tattgaacaa attatgcaat attctagcaa
    23821 ttatatggag tttttaagta agtcaaagac tgaaagactt tcagtaaaag aaattataaa
    23881 attggctaaa gaaaataact atatttcaat agatgaagct atggaaaaag gaagcataag
    23941 ttgtggtgat aagatttatg ttataaataa agagaaggca gtagcacttt ttgtaatagg
    24001 taaaaattat atagaaaaag gtatgaagat tataggaagc catatagatt cgccaagatt
    24061 agatttaaaa ccaaatccac tgtatcaaga gagtaatcta ggttttttta agactcatta
    24121 ttatggtgga ataaaaaaat accaatggac ggctattcca cttgctttac atggtatagt
    24181 tatattaaat gatggaacaa aagtggacat atctattggg gaagaagata gtgacccagt
    24241 attttgtgta actgatttat tgattcattt ggctggagac caaatgcaaa agaaactatc
    24301 agaaggaatt agtggagaag ctttaaatat attaattgga aatatgcctt tagaagatga
    24361 agaaaaagaa cctataacag caaatatatt aaagatttta aatgagaaat acaatatagt
    24421 tgaagaagat ttattaagtg ctgaaattga agtagttcca gctggtaagg ctagagattt
    24481 agggcttgat aggtctatgg tgttgggata tgggcatgat gatagggtat gttcatatgc
    24541 tgctgtaaaa gctatactag aaactgaaca accagaattt acttcagttg cattatgtgt
    24601 agataaagaa gaaattggtt ctaaagggaa tacaggtatg cactcaaagt tttttgaaaa
    24661 tactgtggct gaactgattg ctttagaagg agattattgt gatattaaag taagaagagc
    24721 tttagcaaat agtaaggtat tatcagctga tgtttctgct ggatatgacc caaattttgg
    24781 agaagcatat gaaaagagaa attcggcata tatgggaaat ggagtagttt taactaagta
    24841 tacaggttca agaggaaaga gtggatgtaa tgatgctaat gctgaattta tgtcagaagt
    24901 aaagagaatt tttaataaag gaaatgtaat atggcaaact gctgaacttg gaaaagtaga
    24961 ccaaggagga ggcggaacta tagcccatat attggcaaat caaggtgcag aggttataga
    25021 ctgtggtgtt ggagttttaa atatgcatgc accacatgag attgtctcta aagtagacat
    25081 atatgaaatg tataagggat ataaggcatt ttttaatata aatttataag taaaaatata
    25141 aatgtaataa gtgaataaag gctgttgtat aaacaattta atctgttgta caacagtctt
    25201 atttttatgt aaaaatagca atttgtcaca ctagagaaaa taaaagaaaa tactgtcaaa
    25261 ttaaaaatta tttaaaaaaa aattgacaac gatttcatct tgttataata tgtaataaag
    25321 aacttgttat ataatgttat aaaaatgtta catagggagg tgagcaaaat ggatgaaaag
    25381 agacaaaaaa aagaaatgga tttagaagta aattcctttg ttcctttata tcaacaacta
    25441 tatgacaaca taaaaaaaca aatagcatct ggcatatata aaccaggaga taaacttcca
    25501 tctgaaggag acttatgtaa agaatttaat ataagtcgta taactgtgag aaatgcactt
    25561 aatgaacttg taaaagaaga tatattatgt aaaaaacgtg gtaaagggac ttatgtaaca
    25621 ataccagaga gaatagaagc aacatgtgca ggaaatagtt ttactaattc ttgtcatcgt
    25681 attaatgcaa aaccaagtac caaaataatc tctgttttaa taaaaaaagc agataagcaa
    25741 gtagctgaag ctttaagtat tgaactagaa gagaaggtaa tatgtattaa aagacttaga
    25801 ttaatagatg aagtacctgt tatttttgaa gttgactatt ttagaataga ttacatgttt
    25861 ttattaaaag aagaattaga agataaatct ttaatggagg ttatatctaa taatataagc
    25921 acattaccta aacgagtaga aaatatattt gaagtaaaac attcaaacaa agaatattct
    25981 gaccatctta aatgtgcaag taatatgcca ctattgaaag taagacaatc ggtgtataca
    26041 gaaaataatg atgtcttata ttacaatgag caatttataa gaagtgataa gtacaaatat
    26101 gcagtatcag cagaaatata atctataatt tagtaggagg atgagtatga aaaagataat
    26161 attagcatct catggagact tttcaaaagg gcttttaaat gcagtaaaaa tgatagtagg
    26221 agatttagca gattgtgtca gttcgtatgg tttgtaccct ggacaaagtg cttcagattt
    26281 tgctttagaa ttagaaaaaa caatatcaga agataaagaa tgtgaatata taattttatc
    26341 tgacttatat ggagcaagtg tatgtacagc aatgttaaga ttaactaatc tacataatgt
    26401 taggttattt tctggaatga atttgaatat ggtattagaa cttcttacaa ggttttctga
    26461 tgaattaaca gttgaagata tagaccaatt agtggaagaa tctagaagag gaatacaaag
    26521 cgtgacatta caaattaaag aagaagaaga ggtattttag tttatgaaaa aaaagggaat
    26581 attatcagta tttaaaagta aaaaaccaat tatagcaatg atacacttaa aaggagatac
    26641 tccagaagat atctttgaaa gagctaaaaa agagattaca atctttgaag aaaatggtgt
    26701 agatggaata atgttagaaa attactatgg taattattat gatttagaaa gaatacttga
    26761 atatgtatca aaagctaatt tatcaatacc atatggagta aactgtttaa atgttgatac
    26821 tatgggattt gaactggcaa caaaatataa tgctagttat atacaggttg attctgtagt
    26881 aggtcatgtt aaacctagag atgaagctac tttagaagag ttttttaaat tacaacgttc
    26941 aaaatgtcca gcgtatttaa ttggaggcgt acgttttaaa tatcagccag tattatcaga
    27001 aaatgatgtt gaagaagatt taaaaatagg aatgacaaga tgtgatgcta tagcagttac
    27061 agaaaatgct acaggtcaag aaacatcaat ggaaaaaata gaactgtttc gtaaaaattt
    27121 aggagacttc ccattggtta tagctgctgg agttacatta gaaaatgcaa aaaaacagct
    27181 taaattagga gatatggcaa taataggaag ttactttaaa gataattaca aagattttgg
    27241 ggatgttagt gtagaacacg taaaaacatt catggatgaa atcaaaaaaa ttagggagga
    27301 attataatga ttaaattagt aagagtagac cacagactta tacatggaca agtagcattt
    27361 acatggacaa agtttttaag tacagactgt atattgatag caagtgatga cttattaaaa
    27421 gatgaattaa gaatggcagg acttaaaatg gctaaaccat ctaatgttaa gttagtaatg
    27481 aaaagcatag cagattctat aaaagcactt aactcaggtg ttactgataa atacaactta
    27541 ttgatacttt gtgaatctgt agaagatgtt tacagacttg ctaaagaggt aaaagctatt
    27601 aaatctataa accttggtgg aacaaaatca gatgataatc gtgaaaatat atctaaagca
    27661 gtgcatgtat caaaagatga tataaagatg attaaagagt tagattcaga aggcgtaaat
    27721 gtatttgtac aattagttcc tgatgatgat gctacaaatg taatgaaatt aatataattt
    27781 tagggggatg aaaatggaat ttacacaagt tattttgata actctaattg cattctttgc
    27841 atatatgcat agttttgtag gttctacaat gcataataga cctatagttg ttgcaccact
    27901 tgttggttta gcactaggta atcttcatac aggtatagta ataggttcaa cattagaatt
    27961 agtatttatg ggtgcatttc ctgttggggc aagtaatcct cctgattttg tgtctggttc
    28021 tattattgcc acagcgtttg taatattaac gggtcaagat gtatcagcag cagttgtact
    28081 tgcagttcct atagcaactc tagtattgct tatagataac ttcttaatga cagtagtact
    28141 tacatgggga gctcatattg ctgaccgtta tgcagaagag gggaatattg aaggcgtaga
    28201 acgtgtgcaa ttattatttg gtataggaaa taaattaata ttagcaataa tagtaggtat
    28261 aggattttca ttaggtgttc cagtaataga aaaaatactt tcatttatac catcatatgt
    28321 aactcatgga atggatgttg cagcaggcgt gattccagca ataggttttg ccatgttagc
    28381 tagaatgatg cttaataaga aaacagtagc atttttatta ttaggattta tattggttgc
    28441 ttacctaaac ataacagtaa caggagttgc attgtttgga ttagcaatag ctttaatata
    28501 tgttaacttt gtaggagaga aggaggtaat agtagatgac aacgagttct aagaaattag
    28561 agacaatttc accagatagt aaaataacga ggaaagactt ttggaaatgt tttagaaggt
    28621 cattgacatt agattcatct tggaactacg aacgtatgca aaatatagca tatgcataca
    28681 tgatggcacc aataatccgt agattatata aagatgataa agaaaagaaa tctaaggctt
    28741 taaaaagaca tttagaattt atgtcagtta ctcctcatat aagtactctt ttggtaggta
    28801 tatctggagc tatggaagaa gaaaatgcaa agaataaaga atttgatgct aatagtataa
    28861 atgctgttaa atcaagttta atgggtccag tttctgggat aggagattca tttttctggg
    28921 gaacattaaa gcttatagca gctggggttg gtatagcttt agcttctcag ggtaacataa
    28981 tgggaccgat tttattcctt ttaataataa atgttcctca ttttattata cgctatatat
    29041 gtctagataa aggatttaag tatggaactc aattctttaa agatgtgagt ggttcaagta
    29101 tagtttcaaa agttatggaa gcagcttcaa tgttagggct tatggtaatt ggaggtatga
    29161 ctgcatctaa tgtaatgtta aaacttagtg ttaatgtagg tagtggagaa tgggcagagc
    29221 ctattcaaac ttacttagac caaataatgc catgtatgct tccagctatg atattcggta
    29281 ttatgtattg gttattagga aaaaaggtaa agacaactac gatattaatt tcagttatga
    29341 ttatttgtat agtattggct gctattggag tagtataaat aaaaaaatcc caagttcatt
    29401 tgaacgaggg attttttgac taaattaaat aaaaaagaaa gatggttcat aataatttat
    29461 aagcatttat gtgatagtta taattagctt attaattagt tataaactca aaattaagca
    29521 ctactttatt aaagttatta tattataatt tcgaaatact cacataatct gtttagaaat
    29581 ttatcttcta atggggaaaa agttttcttt ttagagtaaa tgaagaaaaa ctttctagta
    29641 aaatcaacat cctttatttt ataacatttt attttacctg cattgagata atcgatagct
    29701 gaaatgtacg atataaagga cactccaaga cctaatctaa ccatttcctt aatagcctca
    29761 tttgattcta catgagcgat tacatttaat ttacttactg gaaagttatt tttactaagt
    29821 gtgtttagta ttaagtttct tgtacctgac ccttcttttc tcataataaa atttagataa
    29881 gccaattctc ctatatcgat acaattattt ttattatcaa ttttaaaatc tgaaggagtg
    29941 ataagaacaa gttcatcatc taataagtct aggtactcta tttgaggatt attgatttta
    30001 gaaccaacta atccaaagct tattcgttca tttaaaattt ctgatatagc atattgagaa
    30061 tcataatggc ttatggaaaa ttttacatct ggataactca tagaaaaact cttcataaaa
    30121 tcaggtaaaa tataagtttc aggtatggaa ctacatgcta tatctataat accttctatt
    30181 tttcctgaat attcttttat atcataaatt gcacgtttgc aattattcaa tatatatata
    30241 gcatgttcat aaagaatttc accagatttt gtgagtgtaa taactttgtt gcttctattt
    30301 ataagaacag tttctagctc tctctctaaa ttctgtatgt gtgcgcttac agtaggttga
    30361 gttaaaaaaa gttctcttgc agcctttgaa aaactctgat gttttgcaac agcaacaaat
    30421 acttcaagtt gtttaaaatc cattatttaa tcctccttaa attatctaaa actattataa
    30481 catatttgat aaattaatag tcaaaggtga tttttaaaat agaaaacctt taaatattca
    30541 tggagttttt gattgtttag gaataatttg gtataatgtt gtaaggtact tacaataaaa
    30601 atatcgtaaa ttgaattttc aaaattcaac tttaaagtgt tttttattac agaaaaatta
    30661 caattagctt cttaatctta ttaaattcaa ataaatatta tataattatt ttataatata
    30721 ataaatataa ttattgaaaa aagagataac tgtaaattga gatatagata aatactataa
    30781 aatttaagaa aggaggattg atttatgaag ttaaaaaggt cattagtttg tgtaactatt
    30841 ttaggtatta tacttgttgg atgtcataaa gaaaatacta aagagaaaaa tcaagtagca
    30901 agtaaagcaa cacagcaaaa aactatgaca aaagttcaaa atgatgttaa tgaaattatg
    30961 aataaagatt ataagtacat tataaagaat atgggtatac cttataatac attttattat
    31021 ataaaaccta aagttttaaa agaatcaaat acaatgcaag atataaatac atcaagttat
    31081 atgacattag tttatccaaa atatactgga aatgatgaac tagatggaag tgctttatat
    31141 gttgatatta acgaaaataa agttgtcaat gttgaaacca attctttctc atcacaaggt
    31201 atatcagtca ttgatgctga atctagtata gtgattgaaa aaagtgacca tgaaaaatcg
    31261 gctgtatcac tagaaaattt tagacatatt gacctgggag agtatgttgg tgttgaagat
    31321 tccagaataa atgaaattgt aggagatgcg aattatgatt taacagcata taatcatgaa
    31381 ggcagtaaag tagttaagag ttatagattg aaagaagata acaaaatatt aaaaaaagag
    31441 gtacttacaa taagtattgt ggataacaaa ataaaatcta taaaaactat tgaaagtgat
    31501 aaaattgtaa aaataataaa aggaacttta ttagaataaa atattctaaa gcatatgatg
    31561 ctgtttcaag atagaaggaa atttctagct gggaacagtt tttttattag atttataaaa
    31621 tttgaactgc acttatagat aactattagt tataattaaa tataagataa aagtatttcc
    31681 aacttttata atctataata tattacatag aaatatctta aataaaaaat atattgggga
    31741 gtatttaaaa tgatagaatt agaagatgcc ataaaaatta ttgaaaaaaa tgttttacca
    31801 ataaacaggg tcaagatagt aaatcttata gatgcagtaa ataaggtagt tgcagaagat
    31861 atatattcaa caattgatag tcctccattt gataggtcac cctatgatgg ttatgcatat
    31921 aatgcaaatt ctgaaaatag aaatttaaag gttataggga ctttgtatgc aggagatgta
    31981 tttaataggg tattaaataa aaatgaagca gtgaagataa tgactggtgg aaagatacca
    32041 cagggtgcaa attgtgtaat aaaaaaagaa gatgtaacag ttataagtga tatgataact
    32101 ttaaatataa aattaaagga atacgataat tacatattta agggtgagga tataaaaaag
    32161 gggcaattga ttattaataa aaatcagaat ataggatatg atggtattgg agtattagca
    32221 agtcttggaa tatccaaagt aactgtttat gatgatttaa aagttggaat tttgaataca
    32281 ggtacagaac tacaagatat aaatgagact ttagagaatg gtaaaatata cgatagtaat
    32341 agatatacct tatattcaag attattaaaa cttggaatac aagcagatac aatatgcaat
    32401 tgttctgatg atatattgga gttagaaaaa aaggtaaaga atatgttaca gagtgtagat
    32461 tttttaatta ccacaggtgg tgtttctgtt ggagataaag atttaattcc agatgtattc
    32521 aaaaaaatag gggcaacaga attattttgg aagataaaca ttaaacctgg tggagcttgt
    32581 tatgtaggta cattgggaga aaagctatta tttggtcttt ctggtaatcc acatgcagca
    32641 atagtggtat ttgataatgt gatagttcct gttattgagt atttaacata taagaaggga
    32701 acacatcata taaaagctat atttaaagga gaatttaata aactttcaaa taaagataga
    32761 tttgtaagtg gaaaactata tacaagtgaa ggtaaattgt atgtagagct aaatgataag
    32821 aaagatgcaa aagctagatt atctgcaact ctaagaagta attgtatgat taagattaaa
    32881 aagaatgaga caataaaaga aaatcaatta gtggatgttg ttattagata gttttatgaa
    32941 aggacacaat tttatgtata atatattgtc agttgtctca tattcaggag taggtaaaac
    33001 tacattgatt gaaggcataa taaaagaatt aaataaaaaa gggtatcgtg ttggagttat
    33061 taaacatacc tgtcatgatt ttgatatgga tgaggaagga aaagatactt ataaacatag
    33121 aaaatctggg gcgagaaaag tctgtataat atcagataaa cgagtagcat atattgaaga
    33181 actaaaagag aagaatccat tatataaaat gattcaatta tatgaagata tggacttgat
    33241 tatcatagaa ggatataaaa attatagatt taaaagattg gaagtaacta gaaaaggtaa
    33301 gtatgaggat attttatcta ataagtctga tttgataggt attgtcagtg atatagaata
    33361 taaattgaat attgaacagt ttaatttaaa tgattataaa aatatatcaa agtacataga
    33421 gtcttgtata aaaaatgaaa ttttaagtat aagcaatgtt gaaataaaaa acatattaaa
    33481 agaataattt taagatgttc ttcttatata aaatgatata attatttata taaataaaga
    33541 tataagaaat ttatcaattt tattttgagt tataaaatat taaagaaatt gtaggaggac
    33601 aatatatgaa aaaaatatta gtagcctgtg gagcaggaat cgcaacatca acaatagttt
    33661 gtgacagagt agaaagattg gttaaagaaa ataatataca agcagaagtt gagcaatgta
    33721 aaattgctga atgttcaaca aaacaagaag gtgctgattt aatcgtatct actactatac
    33781 taccaacaac ttatgatata cctacaataa aggcaacagg atatataaca ggtataaata
    33841 caacagcatt agataaaaag atattaaatg cattgaagta gttttgtatt tataaaaaaa
    33901 atttagtatt aaatatagag ttgtatcttt ataaaatttt aaatatagag atatagctct
    33961 ttttatgttg tttcaagtat acaactatat gatataaatc ttcaatttta tgaaaaagat
    34021 tgttattact ttgtaagttt ataaaagatt taaagttatt gttatgttat actaatttat
    34081 gggtattttt tagaagtact aataaaggag ggatttaata cttatgataa aacaatttgt
    34141 caaatattat aagccatata agaaaatttt tacactagac cttatagcag cttttctatt
    34201 ttccttatgt gatttagttt atcctatgat aactagaaat ataatggatg acgttgttcc
    34261 aaataaaaac ttaagaatgt tagtagtttt tgcagtagca cttatactta tatttatagc
    34321 aaaagcaggg cttaactatt ttatgcagta ttggggacat gttattggtg tagatatgca
    34381 agctgatatg agaaatgagg tatttacaca cttacaaaga cttccaaata cgtattttga
    34441 taataacaaa tctggagtaa ctatgtctag aatagtaaat gatttaatgg atataacaga
    34501 acttgcacat catgggcctg aagatttatt catttctata gtaatgttgg ttggttcatt
    34561 ctttatactt atagatataa atattcctct tacgcttata atatttgcta tattaccgtt
    34621 tataatttgg tttgcaatag ctaaaaaaga taagatgaat attgctttta tgaagagtag
    34681 agttactata ggtgatgtaa atgctacttt agaaaatagt atagcaggta tgaaggtaac
    34741 taagtctttt tgtactgaga aagaagagtt aaataagttt gtaagaagca ataaattatt
    34801 tagaagagca agacaagatt cttataaggt aatggcagaa tattactctg gtatgaattt
    34861 atacatggat atcttagagt gggtagttgt aatagctggt ggttatttta cttatatagg
    34921 aaagattact ttgggtgatt ttgctgctta tatactatat gtaaaaatgt ttatacaacc
    34981 tatgaaaaaa ttaataaact ttacagagca atatcaaaat ggtatgacag gttttaaaag
    35041 attcatagag ataatggaac aagaccatca aaaagaagct aaaaatccta ttgaacttga
    35101 aaatgtaaaa ggtgacattg agatagaaaa tatatctttc acatatgaag ataagactca
    35161 agttttggat aatttaagtt tatctataaa agctggtaag actatagcat tagttggacc
    35221 ttctggagga ggaaagacta cattatgtaa tctattgcca agattttatg aatttgataa
    35281 gggtgatata aaaattgatg gaaagagtat aaaagatgtt agtttaaaat cattgagaaa
    35341 aaatataggt atagttcaac aagatgtatt tttatttaca ggaactataa gagataatat
    35401 tctttgtggg aaccctaatg ctacagatga agaaatgata gctgctgcta aaaaagctag
    35461 aatacatgac tttgtagaga ctttgccaga tggatatgat acttatatag gagaaagagg
    35521 cgtaaaatta tcaggtgggc aaaaacaaag aatttcaatt tcaagaatct ttttaaagaa
    35581 tcctccaata ataatattgg atgaggctac atctgcttta gacaatgtaa cagaaagaga
    35641 aattcaagag tctttagagg aattaagtaa agatagaaca aatttagttg ttgcacatag
    35701 attgactact ataaaaaatg ctgatgaaat catagtattg acggataaag gaattgaaga
    35761 aagaggtact catgaagagc tagtaaacaa aaatggagta tatagtaggt tacacaataa
    35821 ctagtataca aattttacac catagctaac atacacatta aacttacaaa cataggatat
    35881 attaagattc ataaagacaa ttcattttat atttataaga tgttttatga ataaaatgta
    35941 tcctatgttt tattttttgc aaattacatt taattctctt tgtaagaata gagttaaata
    36001 gccaatggtc gggataacgt ttttacaaaa agaataattt agaaaactaa ctttaattta
    36061 tggaatttat cgaattttat gaaaaaaatc gaattttgac ttgaaatgtt tttcaaaagt
    36121 agtaaaataa tggataacta tacatacatt ttataaaaat atatttatta tgagggggga
    36181 ttttgtatgg aggcaataac agctttttgt ctagttttag tatcactgac gataggagat
    36241 atagtttcag caaaaactaa agcttttgtt ccgtcggttt ttgtatcagc aattatattt
    36301 atagtaggat tttggacttt tttcccagaa aatattgtgg acttagccgc tttaggaaca
    36361 ccattagctc aattaggtat gcttttgtta ataactcata tgggaactat gatgagtatt
    36421 aaggaattag caggtcaatg gaaaacaata gtaatagcct tggcaggaat agtaggaata
    36481 tgtgtaggtg ctttggcatt agggacagta gtatttggat gggatacagc agtaatagct
    36541 acacctcctc ttactggagg gctagttgca tctataatga tggcagatgc tgctacagca
    36601 aaaggtcttg caagtttatc agtacttgca attttgatgt atgttgcaca aggatttgcg
    36661 ggttatccta ttacagcatt aatgcttaaa aaagaaggaa aaagactttt atcagacttt
    36721 agaagtggaa aagtaactgt aaatacagat gaagaaaaag ttaaggattt acctgaacaa
    36781 aaaagcagat ttaagataat accagactta ccagaaaaat atgatacgac atatatgata
    36841 atacttagat taggatttgt agcatggtta gcagtacaat ttacaaatct tacaaatgaa
    36901 gttgtatcaa gatatgttat atgtttaata tttggtgtaa tagcatcaga acttggaata
    36961 gttgatagaa aaccacttaa taaatctggt tcatttggat ggttaatgac atcattgatg
    37021 gcttttatat ttgcagggtt agcaaaagct acaccagcaa tgcttgttga aatggcagtt
    37081 cctcttgtag gaatcataat atttggtgta ataggtatgg gaattttctc tattatagta
    37141 ggtaagcttt taggtgaaag taaagaaatg gctttttcag tttcgcttac atcactttat
    37201 ggttttccac caaactatat attaactgaa gaggcagtaa aggctttggc tgaaacacca
    37261 gaagaaaaag aatttttaat ggataaaatg cttccaaaga tgcttgttgg aggatttact
    37321 acagttacaa tagcttctgt agtaatagca ggtatctttg taaatctaat atagtattta
    37381 attgataaaa agtattttaa ataagaagaa tgtgtactac ttggaggtat agaatggata
    37441 ttaaagaaat aacaaagagt tataaagact atgtaattaa gcttagaagg gaatttcatg
    37501 aaaatcctga gaaaagtatg gaagaagtta gaacatcaaa gagagttaaa gaagaacttg
    37561 ataaaatagg gataccatat gtatcagctg gaggtacagg tgtaattgca actataaaag
    37621 gagataatcc aggaaagact gtagcactta ggggtgatat ggatgctcta caagttgttg
    37681 aatgcactga tgtagaatac aaatctaaaa atgaaggact tatgcatgct tgtggtcatg
    37741 atggtcacac atcaatgctt ctaggggcag caaaagtgtt aaatgatatt aaggatagta
    37801 tcaatggaac tgtaaaatta ttttttcagc caggagaaga agttggtaaa ggtgcaagag
    37861 ctatgattca agatggtgct atggaaggtg tagatagtgt gtttggaata catctttgga
    37921 ctgatgtaga atctgggacc atatcggtag aagaaggacc aagaatggct tctgctgatt
    37981 tctttaaaat tactgtaaaa ggtagaggtg gtcatggttc acttcctcat caaggtgttg
    38041 atgcagtgct tgcaagttca gcaatagtta tgaatctaca atctatggtt agtagagaag
    38101 taagtcctct ggaaccatta gttgtgagtg ttggagtttt aaattctgga actagattta
    38161 atgtaattgc gagtgaagct atcttagaag gtacaataag gctatttaat ccagagctta
    38221 gaaagcaaat tcctggaatt ttagaacgta tagcaaaatc tacagcagaa gcatatagag
    38281 cagatgctga attagagtat gggtacttaa caccagcagt tataaatgac aaagaatgtt
    38341 caaagatagc tacagatgca gcaattaagc tatttggaga agactgtata acactatttg
    38401 aaaaagtaac aggagcagaa gatttagctg agtttatgaa tatagcacct ggtgcactag
    38461 catttgtagg agctagaaat gagtcaaaag gtgcttgtta tcctcatcat catggatgct
    38521 ttaatataga tgaagatgct ctagaaattg gaactgcttt atatgtgcaa tatgcagtgg
    38581 atttcttaaa taaataattt taatatacta taaagaaaaa gcagagacta agtttatatc
    38641 tctgcttttt tattgtgtat attttttata aatattgact atataaaata tatatgatag
    38701 cataaagaaa atgataatca atatcaatta aaaagaaggt gatttatttg aataatgtaa
    38761 tatttgcatt tttgataaca ttattggctg gtttatctac tggtatagga agttgtatag
    38821 cattttttgc taaaaaaaca aatactaagt ttctttctat aagcttagga ttttctgctg
    38881 gtgttatgat ttatgtttct atgatagaaa tttttcctaa agcacaagat gctttgacaa
    38941 aatctatggg agagaaatta ggaagttggt caactgtaat agcttttttt ataggtatgg
    39001 ctatcattgc tgttattgat aagctaattc cacaagaaga aaatccacat gaaataaaga
    39061 aaatggagaa tatagaagaa aagaatatca aaaaaaataa gtctctttta agaacaggta
    39121 tatttacagc aatggctata gcaattcaca attttcctga aggattagct acatttatat
    39181 cagctcttga tgatgttaca atagctattc cgatagcaat tgcaattgca atacacaata
    39241 taccagaagg tatatctgta tctgtaccag tttattatgc cacaggagat aaaaagaaag
    39301 ctttttatta ttccttttta tctggtatgt ctgagccttt aggagcgatt attggatatg
    39361 tacttcttag aaatttttta aatgatataa ctcttggaat tgtatttgca attgtaggag
    39421 gaattatggt attcatatca ctagatgaat tattgccatc agctagagag tacggggaac
    39481 atcatttatc tatttatgga ttgattgcag gaatgggtgt tatggctatt agtttattac
    39541 tatttaaata acttttaaat atatgattgt aacctaaaat tagtatcaaa atgatataat
    39601 tatttaaata gatttttaag ctatttaaaa atatcatttt ggagaatgag tgctatggaa
    39661 aagaaaataa aattcaaatt tgactggtat agttcacctg ataagataga gaaatggtta
    39721 gaagatattg aagctaaagg atataattta tataagataa ataaatttgg aggaatattt
    39781 tattttcata agggaagtcc aagaaaaata aaatacactg ttgattacta ctatgatgta
    39841 gagaataatt attttgatga acataaatca aatggctgga atcttgtatt taggagtatt
    39901 ggtagtttta tgggtacatc tgcatggtat atatggtcga aagaatacaa agacatacct
    39961 cctaaattat atagtgattc tgataaaaca aataaagtaa agtcaatttt atttatgaat
    40021 atgtttatat tctttatgtt agtagtaata tttatatatt tattgaatat tgaaataaga
    40081 aatttatata gtggttatgg agcatctgta actgctattg tactacagat tatattgttg
    40141 attttatatg cagtacaatt atataggaga ttaatgtatt atttaaggct taaaaagttt
    40201 aaataaataa aagaaggtgt cctaaaatta tttttttaat attttaagac atcttctttt
    40261 gtcagtaagt actgttttta attatagtaa tttctgtctt taaattgaaa tatcaaataa
    40321 tagttgtatg ggattgagta ttgataaaaa taatttaaaa atgttatcct aaagtatcag
    40381 atatacaaaa ttatctttaa atatattata aatattttaa ttgtattaac tagaaattat
    40441 gtaaggaggc attttatgat taagatagca gtttgtgaag atgaaaaaga aacacaactt
    40501 ttaatagaag attaccttga gaatatatta aaagatataa gtatagaata tgagatacaa
    40561 aagtatatat ctggagaaga gttactggaa agtaatttaa aagatataga tatattactt
    40621 cttgatataa aaatggaaaa attaaatgga atggatactg caagaaagat tagagaagta
    40681 gacaatgaaa tggaaataat atttgtaact tcattaatag attatgtgca agaaggttat
    40741 gaggtaagag cttataggta tttactaaag ccaatagagt tggaagaact aaaaaaacat
    40801 gtgctaactt gtatcaaaga tattgaaata aataaggaaa gtcatattac aataaaaaat
    40861 aagtctaata catataagat ttatttaaat gaaataaaat atatagaagt tcaaaaaaaa
    40921 gatatgctaa tacacacaat aaacaagaat tttgatataa aatatagttt aggcaaaata
    40981 gaaaaagaat taaatccata taaatttata agatgccata aaagttttat agtaaattta
    41041 agatatgttg aaaatataaa gcctaatact gcaatactgg aaagtggaga agaggtacct
    41101 attagtagat atagatataa agaagttaag gagaaattct taaaatttct tggtgataca
    41161 atatgctaaa ctttatgact gagctattag tagttttatc tatatttata cagttttcta
    41221 taatgaacgg aattttaatt tataaaaagg atagtaaaaa aaatgaaaaa atagtattct
    41281 atattgtctt gtttcttatt tatattataa ttcagataag tagattattt aatggaggaa
    41341 attactttag taatataaac tatttatttt atatattagc atttattgct tggggaggat
    41401 actataatta tttt
//
LOCUS       DAMJYX010000024        38931 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJYX010000024 DAMJYX010000000
VERSION     DAMJYX010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.024"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(318..980)
                     /locus_tag="PC479_001412"
                     /pseudo
     CDS             complement(318..980)
                     /locus_tag="PC479_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1140..1676)
                     /locus_tag="PC479_001413"
     CDS             complement(1140..1676)
                     /locus_tag="PC479_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2803964.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1749..2162)
                     /locus_tag="PC479_001414"
     CDS             complement(1749..2162)
                     /locus_tag="PC479_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2803965.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2628..3182
                     /locus_tag="PC479_001415"
     CDS             2628..3182
                     /locus_tag="PC479_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2803966.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3790..3969)
                     /locus_tag="PC479_001416"
     CDS             complement(3790..3969)
                     /locus_tag="PC479_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2803967.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4195..4728)
                     /locus_tag="PC479_001417"
     CDS             complement(4195..4728)
                     /locus_tag="PC479_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2803968.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5045..5395
                     /locus_tag="PC479_001418"
                     /pseudo
     CDS             5045..5395
                     /locus_tag="PC479_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6476..7048)
                     /locus_tag="PC479_001419"
     CDS             complement(6476..7048)
                     /locus_tag="PC479_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2803969.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(7110..7373)
                     /locus_tag="PC479_001420"
     CDS             complement(7110..7373)
                     /locus_tag="PC479_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2803970.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7373..7594)
                     /locus_tag="PC479_001421"
     CDS             complement(7373..7594)
                     /locus_tag="PC479_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2803971.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            complement(8110..8646)
                     /locus_tag="PC479_001422"
     CDS             complement(8110..8646)
                     /locus_tag="PC479_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2803972.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(8664..9977)
                     /locus_tag="PC479_001423"
     CDS             complement(8664..9977)
                     /locus_tag="PC479_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2803973.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            10332..10772
                     /locus_tag="PC479_001424"
     CDS             10332..10772
                     /locus_tag="PC479_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2803974.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(10956..11144)
                     /locus_tag="PC479_001425"
     CDS             complement(10956..11144)
                     /locus_tag="PC479_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2803975.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            12166..12561
                     /locus_tag="PC479_001426"
     CDS             12166..12561
                     /locus_tag="PC479_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803976.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            12604..14703
                     /locus_tag="PC479_001427"
     CDS             12604..14703
                     /locus_tag="PC479_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2803977.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(14928..20138)
                     /locus_tag="PC479_001428"
     CDS             complement(14928..20138)
                     /locus_tag="PC479_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2803978.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(20153..22648)
                     /locus_tag="PC479_001429"
     CDS             complement(20153..22648)
                     /locus_tag="PC479_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2803979.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(23309..23596)
                     /locus_tag="PC479_001430"
     CDS             complement(23309..23596)
                     /locus_tag="PC479_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803980.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(23616..23891)
                     /locus_tag="PC479_001431"
     CDS             complement(23616..23891)
                     /locus_tag="PC479_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803981.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(24526..24705)
                     /locus_tag="PC479_001432"
     CDS             complement(24526..24705)
                     /locus_tag="PC479_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803982.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(24990..26465)
                     /locus_tag="PC479_001433"
     CDS             complement(24990..26465)
                     /locus_tag="PC479_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2803983.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            27496..28674
                     /locus_tag="PC479_001434"
     CDS             27496..28674
                     /locus_tag="PC479_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2803984.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            29024..29905
                     /locus_tag="PC479_001435"
     CDS             29024..29905
                     /locus_tag="PC479_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2803985.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            29890..30263
                     /locus_tag="PC479_001436"
                     /pseudo
     CDS             29890..30263
                     /locus_tag="PC479_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(30523..33090)
                     /locus_tag="PC479_001437"
     CDS             complement(30523..33090)
                     /locus_tag="PC479_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2803986.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(33093..33698)
                     /locus_tag="PC479_001438"
     CDS             complement(33093..33698)
                     /locus_tag="PC479_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2803987.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(34072..35532)
                     /locus_tag="PC479_001439"
     CDS             complement(34072..35532)
                     /locus_tag="PC479_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2803988.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            35902..36480
                     /locus_tag="PC479_001440"
     CDS             35902..36480
                     /locus_tag="PC479_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2803989.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            36771..38021
                     /locus_tag="PC479_001441"
     CDS             36771..38021
                     /locus_tag="PC479_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2803990.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            38182..38751
                     /locus_tag="PC479_001442"
     CDS             38182..38751
                     /locus_tag="PC479_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2803991.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT        13237 a         6631 c         4666 g        14397 t
ORIGIN      
        1 atagcttgta gtgtacttcc ctcaagagga atagtcgttg aagtttctcc tattcggagt
       61 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
      121 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      181 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      241 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      301 ttcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      361 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      421 ttatggactt cttcaagtga aaacataaca cccgctagtg gtgcatgaaa ggctgctgat
      481 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      541 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      601 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      661 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      721 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      781 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      841 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      901 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      961 tgagatgtat cctttttcat actattcctc cttcatccaa taattctaca caatctgaat
     1021 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1081 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1141 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1201 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1261 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1321 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1381 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1441 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1501 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1561 tcaatagcag tcttatccca tatacttttt aataatataa tgacatcttt tgcactttta
     1621 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1681 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaatag
     1741 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1801 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1861 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1921 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1981 aacccttttt gacagacaca atcctttatt ttgcacttac ggcagtgttc tggctttcct
     2041 gtatcgccgt tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2101 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2161 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2221 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2281 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2341 acacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2401 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2461 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2521 gttttgaata gtagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2581 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
     2641 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2701 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2761 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtagtatg aatggcacta
     2821 ttagcgatat gcaaggcagt atcagtagta tggatggcac tattaatagt atgcaagaca
     2881 gtattactga tatgcaagtt gaaattaaag aagtcaatag taggttagac aatatggaac
     2941 ctgatattaa agacttaaaa actggacaag ttgaaactag tagtagactt gatactattc
     3001 agagtgatgt tacagaaatt aagtcagatg taaaaattaa cagaaataca ttagatacag
     3061 tgattaaaca tacctcttca ttagtaaaag atacatcaga tattaaaaat gatttaaata
     3121 atatagagat agtcacatct aagaattcga atgatattgc taaattaaaa tcagttaaat
     3181 aatatactat agtaaaaaat acctgaaaat ttcacatact tctttaaagt tataccaaat
     3241 tttatacaga aatactgatt atccgacaac acactttact tataaataat ttattaaaaa
     3301 aatactcatt ttatcctaaa attaacacga ctaaatgagt cttgatataa taataaaaaa
     3361 taagggagat tgaacactaa aaagttgtgt atctccctta tattctataa tatagttata
     3421 taatttttca gcctaaaata aaagtccatc tttgattaaa acacatcccc ttatcactta
     3481 taaaaggata aaatcttgtt ttcatttttc catttatatc ttcttgctta agagtaataa
     3541 ttttttcttt tgatacttta agtgattggt taaatttaac caaacgacta agttttgttc
     3601 aacatctatt tttcatattt tccaatactt aatctatttt ttataaataa ttcttttatt
     3661 tctaactcac ttacattttt tacataaaac gaagcatata aatttcaacc ttatcttgta
     3721 tggttcttaa tacttaatga gtagcttaag tatcaatcat tataaatttt tcccttacat
     3781 ttaacactct taccatttcc aaaatataca aaatatattt tgaacatttg tacaatgatg
     3841 aaatacaata catgaaaata caatatcata actgttgtct aaaaatagaa gttcttcaac
     3901 ataacctttc acaaaaaaca tattatctaa gtattttttg tctgctcctt gttttcctgt
     3961 ttgtagcata taaattactg catctaaaca tataactact cacacgaaaa aagaaaataa
     4021 acttccaaaa tacaagctct aaaaactacc tctaaagttg tataatgttt actaggaata
     4081 aggttgtata acatatagtt taaataatca ttttttaaaa ttgcaagtta ttgctttcaa
     4141 aattagatgt ttttatatca aataaatgat taagaatttc taaatttaat ggctttataa
     4201 tgcactccct ttctttggga taaaataatg ttgcacagat actttttcta aagataacaa
     4261 ttttattttt ctatcaattc caccagcata cccagtcaaa ctcccatttg ttcctacaac
     4321 acgatgacat ggaataataa tagagattcc attatgccct actgcaccac cgattgcttg
     4381 tgctgacatt ttagatattc ctttttgttg agcaacttta tatgcaattt ctccataagt
     4441 gattgtttta ccatatggta tttgttgtaa aattttccac actgtagtct gaaaaggagt
     4501 acctaccata tggagtgatg gtgtaaaatt gggttctttt cccgaaaaat aaatatctaa
     4561 ccatttcttt gtctgctcga aaattggcaa ttctttttct tgatgttctt ggtcaagatt
     4621 ataagcataa tacttttgat tctcaaacca aagaccagtt aacccaatat tatctgatgc
     4681 taaaagaata tttccaattg gactatggta atagctaata tattgcatac aattcctcct
     4741 tatattctga aaacttcact caaatcatta gacacttctt ttctatctac cattatgtaa
     4801 aaacatacta atataatttc cttctaacta cactttcgtt ataatatcta tcctcatatc
     4861 atattagttt tatatttacc tgcatctgtt catccttttt tccctttccc tttgatgaat
     4921 ataaaattca tacatttaag cttcctactg tattaacttt tatagaagtg tatattataa
     4981 ttttatcact aaataggcca aaaaaatatc gatatagcat caagtaatat cataatactt
     5041 tcaaatggga aaagtaagaa taggctgtaa aaaagaaaag tatttttatg ttagaaatcc
     5101 agtaaaatca ttagtagata gaataacaga aaacgaacaa tttgctattt atcattttac
     5161 acataaatat aattgacgca ataaagtgtt ttctattcta caacgattaa taaggttaga
     5221 aaaaagtaaa aatgaactgt atatgtatga agcattagtt tctgtttctt tcctttggct
     5281 tattatgata aaaaatattg ttttgctacc aataaaacac aaaaatataa ttaatatcca
     5341 tatgcaaaaa attttacgtt atattgaaaa atactattgt tttacattat attgaagggt
     5401 actattgtga aaacttaatt ttggagtact atcaagcagt ggtaatatca ataaatcaga
     5461 gtgctccaga tgatttgaac taagtttaaa tactatacct tataaatatt ttactgagtt
     5521 ttgtttatta aaagcaactc aatttctaaa aggcacaaat gagctaataa gtgacattgc
     5581 tataaaagtt gtttttcatc aaataatcta ttttagtaaa tgttttaaag aaaaaacggg
     5641 atattctcca aaagtgtaca gaaatataaa aaaagagata tgatttttta tttcttttga
     5701 ttatccaaag agtaaataaa aaatattgta aaaaacttac taacaacggc aatgctttga
     5761 actatggtaa tactttgaac ttttgattgg actttttttg ctaaaataat atatttaaat
     5821 agcaaagtaa tctgtttaat caataaaaaa taagagataa aagaaatcaa ctaaaacttt
     5881 tacctcttat tttgatagtt tatcagagct aatatttaca ctctttttat ttttgatact
     5941 tcattaaaaa tgatgcttac tctccattca cttttaattc ttcattaaaa atgatgctta
     6001 ttctccattc acttttaatt cttcattaaa aatgatgctt actctccatt cgcttttaat
     6061 tcttcattaa aaatgatgct tattctccat tcacttttaa ttcttcatta aaaatgatgc
     6121 ttactctcca ttcgctttta attcttcatt agaactatgc ttactctcca ttcactttta
     6181 attcttcatt aaaaatgatg cttactctct gttcactttt aattcttcat taaaaatgat
     6241 gcttattctc cattcacttt taattcttca ttaaaaatga tgcttattct ccattcactt
     6301 ttgattcttc attaaaaatg atgcttattc tccattcact tttaattctt cattaaaaat
     6361 gatgcttatt ctccattcac ttttgattct tcattaaaaa tgatgcttat tctctgttca
     6421 cttttaattc tttattaaaa ctgatgctta ttctccattc actttcaatt cttcattaaa
     6481 actgatgctt gcactctgta aactcataaa catccattaa tgcttcacca aatctttgat
     6541 agtgaactac ttccctctca cccaaaaatc tcaatatatc tttcaaatca gggtcatcag
     6601 ttaaatttat taattgatag tacgtagcca atgctttttg ttcagcagcc atatcctcat
     6661 gtaaatcagt taccgaatcg ccaaagacat taatataact tgtagtaaag tttactccat
     6721 tagaatctac tggctgaaga ccatgtccaa aattagcata attactacca agtccagcag
     6781 ccttaagctc ttctggagta caattgccta ttaattgata taacactgaa gaaattatct
     6841 caacatgagc catttcctct gtacctatat cagttaaaag tgcacgagat tttccagttg
     6901 gcatagtata tctttgttga agatatctag ttgcagctgc caactcacca ttaggtcctc
     6961 ccaactgagt catgatatat tttgccattc tagggtctgc ttgtcttatg ttaactggat
     7021 gttccagtgt tttttgataa atccacatat tatcttactc ctccttaata tatatttaat
     7081 aataaatcta attttaaagt ttaataattt tatttataaa atgatttgtc ccatggccaa
     7141 ggttcagata tccatgacca aggatattca cttgattcat atccaaaatt agttaatgct
     7201 ccatatttag attcataatc acacacagct tgtgtaaatt tatcgcagta tctgttgtat
     7261 gaatcaagag ctttcttatc ccttggattg ttatctaaat ataaatttaa ttctatacag
     7321 gcaaattgat attctgaaat tctctctaaa agttctcttc tatttaatga cattagtaca
     7381 tgttaccccc ttttttagct ctaactctat ttttaggatt tgcatatagc caatcattat
     7441 agttatttga ctctattaaa tctaattcag gaaataaagt acctcttttt aatgctgttt
     7501 ttggattgta tgtgtcatca taaatttgac gattgatgta tggtctagca aattcacaac
     7561 aatagtcttg tttatcctct tttaaatatc ccattaatta tcctccttaa tttttagaat
     7621 tttaaaatag cattattttt aaaaattgta tactggttct atattataac tggtagtttt
     7681 aataggtctt taataagatt aaatgattaa ataagtatct ttttttctta ttttacctct
     7741 aaaatttttc taattaatct ttcataaata tctattttaa ataaataata tagagtttat
     7801 acaatttttt atagcttaat tactatttac tatatattat tcatttttaa ataaaatgtg
     7861 attatttttt attaaaaacg atataaatat acaatttaat actatttttt tataaataaa
     7921 cttgtattaa aatacattta gttatataat tttatttttt ctaagagtat aattataaaa
     7981 ataaaaaaag aataggataa ttttcctact cttttttctg tacttacata taatattaaa
     8041 actctttatt ttttaatata tattttaaat tttctttttt aataaatctt tttcttttaa
     8101 taaatctatt taattttctt cttctatatc agagccaaag ggaatccatg cattaccaaa
     8161 ctctttatct ttaaataaat aagctaatcc agttatttgc ttcaatctaa gaatttcatc
     8221 aacttccata cccaggtgct ttgatatcca ttcatcagat tttccaattt cacaaagttc
     8281 agttataatt ttactcatta aatccacatt atgagaacct cttgcacgat tgtgtcgaat
     8341 agtacttgcc attctttcat caagagattt atcaattact gaaacaggaa gaagtccatt
     8401 ttctctatca tatatatcct cattttcaag cattactcta tatctatgaa aaccatctac
     8461 aataatatac ttatcctttt cctcattata ataacagaca attggcattg tatacccatc
     8521 ttcttttata cttttataca acaactgcat ttctggtggg gctactgcat ttggattata
     8581 gtcgttagat tctatctttt cgattggcac tgcgatgatt ccatatacag gacttttata
     8641 ttccatagca tcttaactcc tatctaatga attttttcat atttcttctt aatatattcc
     8701 gttctaattc tctgttcttt tgtaggacca aatcccataa atctacacat atggtcattt
     8761 tttaaaatac aataacacat tcttttccaa cttggtatat ctttggtaaa tttaatatca
     8821 tccgtatcat caggtatatc atccaaaaat atgactctac tctttttatc ttttgtatga
     8881 tttgaaactc catttaattc tattttataa ccttttctca acaattcacg tataactatt
     8941 tctgacaaac caccacctgt attctgccaa aactcaatag atgttgaaaa ttttttaata
     9001 tagctattcc ttatattttc aggtaaggta tcaagcaaaa aatatgtgaa acttttccat
     9061 gtgtgtccat atggcaattt aacactacga tatcccatag cttttgtttt gccataaata
     9121 gaagtaaaat taactccttg tactctagca accaatttca cccatacatc tgggtccaat
     9181 attctgtata aatttaaact ctcttttgct gaatcattaa aaggtgatgc cactctcatt
     9241 tttcttggag ctactccagc tttatagaat aagtcataaa ttttattata gtcatagtca
     9301 aatttatagt gtgctaacca tatatcttct atttcccaat catatatagg agatgccgtc
     9361 cagacatttt taaattcctt agttatccag cactttcctt tatatccatg ctgtttattc
     9421 acaatagaac aataacgttg tagagattct gctgctcgtg ttcctaataa gcaaacaact
     9481 tttgcacctt catgtatatc tctaatccat ctaccaaatt gttttgccaa actttcttgt
     9541 ctcattttat atttataata agtaaaagga ttattttttt gatttattat gtattctttg
     9601 actggcattt cccttaccca taactcttct ttctcatcat cccatggata ccaatacatt
     9661 tcataatttc ctacagcagt tcttgtagcc attggtaaac acacccaata tggttcaata
     9721 tcttcaatat tatcttcaaa tactttttca acatattcag tagtcaaact atattgagcc
     9781 tcaaaatctt gatgaaatac tcctattttg gaacttatat tattctttct cttataatcc
     9841 aaaactaaat ttagcagtaa accactatct tttcctccgc taaaagatac atagatataa
     9901 tcaaattcat taaaaagata agaaagtctt tcttgaaaag cttcaaatac atcggattct
     9961 aaatatctct tatccatttg aattatcccc tttcaattag aagtttataa aaccttacaa
    10021 tgcaatatta ttatgctttt ttagcagtaa tcagcataaa ttatacatta tttaaataca
    10081 caaatccaac taactacata taattataat gtaattatac ttttgtatta taaactatat
    10141 tttatcatta ttttacattt ttttccaata tatataaata tcatattttt ctattagcta
    10201 aaactaatta ctttatattt ctacgtatta gtataataat acataaaaac ttgaataatg
    10261 cataaaaact tgacatactg ttgtcataat tactttgcta taattaaaag taaaaatgga
    10321 ggtaaattct aatgtatgaa cgtatgcttg ataaaagcaa taaaccctgt ttgtcccaaa
    10381 taatagaata tataggtaaa gatggatatg cacgattgtg taagttggaa acttacttga
    10441 aaactcaata taatttatca agagaacttc gatttccttt tggaaacaat tatggttggg
    10501 gttacaaata tagccataaa tcttttcatt tattttatgc tttttttgaa aaaggtgcat
    10561 ttactgttct gattcagata ggtgacaagc aagttccagc agtagaaaaa acgttggtag
    10621 ctttgacacc aaaagctaaa gaactctggg tcactcgtta tccttgtggc aataatggag
    10681 gctggattaa gtatcgtatt ttagaagatg cagacttact tgatattatt caatatataa
    10741 gagcaaaaaa atctccacct aaacaaaatt aacttttatt ttttgtgatt taaatatagt
    10801 gtatataaat tccattcttt ccatattaaa aaataatata aaaaatatct attagtataa
    10861 cttatttagt atgtcttatt ctacttttta cagataatta aaaagtttta tcataaataa
    10921 atcataacta gacatcctag taaatcaaaa ttaatttatt cttttttatt cgaatttttg
    10981 ataatagtcc ctatacatat gccaattatc attccaaaaa ttagaccata acacattgta
    11041 atacctacat ttcctttaga aatacctata gcacaaccta tagccattcc aagtatcatg
    11101 cctataaaca tacatataat aactaaattg ttgtttttat tcatagtttt tctccattca
    11161 atatatttta aattgcctag ctcgccttct tagcttaaaa caaagaaata taaattaact
    11221 ttctttcatt ctaatatctg cctgtataaa tgtcaaacaa cacatttctt gtcataacct
    11281 cctctttttg aagtaagtac aatctcccag acgcaataat atcaatttcc taaaaccatt
    11341 taagtaactt ttatatttac ataatattta caatcataaa ctatattgtt attcatttat
    11401 acatcataaa cactccctcc taatcattat tatacttaaa ataatgttca tttataataa
    11461 tatatctaac gcactttaat tgaaaaaact aaaactttgc ctttcattcc catatataaa
    11521 taagtatact ttttgatgct ctattacaaa tcaactaaat taattgatat aaattatcat
    11581 tttctgttga aaataccatt cgcacatgct atacttaatt gcataaatgg taataaatgt
    11641 catatgattt tacaaatcta ttaataacaa ttattacttt tactgtattt taaagttttt
    11701 ggactaaata tcaagaactt tctaaaatac aaagtacaaa aaatttatat aagttataaa
    11761 tttatatgaa ctagataagc tcaaacaata tgttatgtat aaactgtgta gtattcggtt
    11821 ataaatttat ataaattata taagctagat aaagtattac ctcaactata taagctagat
    11881 atataaattg cttatataag ctagataaga attatttcaa ctatataaac tacatatata
    11941 aattacatac ataagctaga tatataaact gcatatataa gctatataag aattatttca
    12001 accatataaa ctacacatgt aaaattcata taaatttact aatataaatc aaataaaatt
    12061 actaaactct ctaaactaat tttgtaaaac atactctatt tttaacattt gttaccttat
    12121 acataagtac aaacaaaatc taactaaatg aaaggataat gtaaaatgaa tattttaaaa
    12181 atgaaaaaaa cagggatatt tttaggagga gtactttttg gtactgtagg tgttaagata
    12241 ctttcaagtg attgtgttaa aaaaacttgc actcgtgcaa cagcaggagc attgaaagtt
    12301 aaagattctg ttcttgaaac aactacgaaa attcaagaaa atgtagaaga tattttagct
    12361 gaagcaaaag aaatcaacga gcaagaagac ttatctacat ctaaggtaaa cgttgtacaa
    12421 gatactctta aatgcgaaca agaagttctt aaaactgaaa ctataaaaac tgtagaagat
    12481 actaattcta atagcattaa atgtgaagaa atcaaagata acgtaattga agaaaaaaat
    12541 agtacttgta caacagaata aatttaatga ttattatgat taataatatc aggaggaagt
    12601 aaattgaatt tttatataaa acacgaaata agaggtcgta tacgctttga cttacaactt
    12661 ggcaaattaa acaacaagca gtccgatact ctcttatatt atttaacttc tataaatggt
    12721 attaccaaag caaaagttta cgatagaact ggtgatgcag tcatttatta cactggtgaa
    12781 agagaataca taataagaga aatcactaaa ttcagtttta acaatagcaa aatagaaaac
    12841 ctagttcctg aacataccag cagggaatta aacaattatt atagagacaa attagcaaat
    12901 aaactgctag tcaaaagtat ctccaaaata ttttttccaa agtccttaag atttttttta
    12961 attggacttc attctataaa atattttaaa aaaggtctct cttccctgct aaataaaaaa
    13021 atagaagttt ctgttttaga tgccactgct ataggtattt ctatttttag aaaagatatc
    13081 aatacggctg gttcagttat gtttcttctt ggtatcggtg aactattaga agaatggact
    13141 agaaaaaaat ctatagacga cttggctcaa agtatgtcac taaacattga aaaagtatgg
    13201 ttaagaaaag gtgacactga aatcttaatt ccaatttcag agataaagga aggagattta
    13261 gtaagtgtaa ctatgggaaa tatgattcca ttggatggag ttatagtatc aggagaaaca
    13321 atggtcaatc aggcatcact tacaggtgaa tcccttgccg ttaacaaaaa agaaggctct
    13381 tacatatatg ctggaactgt aatagagcaa ggcaatattg ttatgtgcgt caaggaaaaa
    13441 gctggtacta ctagattcca gaaaatagta actatgattg aggaatctga aaaactaaaa
    13501 tcttctgtag agagtaaatt tgaacatcta gcagatacat tagttccata cagcttttta
    13561 ggaagtatat taacctatgc aattactaga aaccctataa aatcactctc tatattaatg
    13621 gtagatttct cttgtgcatt aaaactctct atccctattt cagtactatc agccatgaga
    13681 gaatgtaaca ataacaacat tactgtaaaa ggtggaaaat tccttgaagg tgttgcatgt
    13741 gcagatacta ttgtatttga taagacaggc acattaacaa aagctcaacc aactgtttca
    13801 gatataatta cattccaaaa ttataataag gaggatatgt taagacttgc agcttgctta
    13861 gaagaacact ttccacactc catagcaaat gcagtagttt acgaagctga aaaacaaggg
    13921 cttagtcaca aagaaatgca tacagaggta gaatatatta ttgctcatgg catttcaaca
    13981 aaagttgatg ataaaaaggt tgtaataggt agcaaacatt ttgtatttga agatgagaag
    14041 tgtactatcc cagacggtga ggaggataag tacaacaatc tatcagatga gtattcacac
    14101 ctatttatgg caatatcagg taaattatca gcagttattt gcataaatga ccctttacga
    14161 aaagaagcaa aatatgtaat ttcaaattta agagaatgcg gaataaaaaa gattgtgatg
    14221 atgactggag atagcgaaaa aacagctaaa tctatagcaa gtaaagttgg tgttgatgaa
    14281 tattattcag aagtcttacc agaagacaaa gccaattttg ttaaaagtga aaaactcaaa
    14341 ggtcgcaaag taattatgat tggtgatgga atcaatgatt ctcctgctat ttcagaatca
    14401 gatgtcggaa ttgcaatgag tgaaggtgct gaaatagcaa gagagatttc agatattacc
    14461 atatcagcag ataatttgaa taatttaatc gttttaaagc agattagtaa taagcttatg
    14521 aaaagagttg atttaagttc taagtttatt attggcttta atttaggtct gattttactt
    14581 ggtgtaggtg gatttgttag accaagtaca tctgcctttt tacacaacac atctactgtc
    14641 ggaataagtt taaacagtat gacaaattta ttggaaaaca ccaatactta taactaccat
    14701 taatctccat tacataaaac atatgatata aaaaatttat aattcatctt tttaaaagaa
    14761 tatgtcttaa aattagcttt tttctaatca aaattatata aagcatactt tacaaatttt
    14821 aaactcaact tatttctatt ttttacaaaa aaggagatga ttcaaatttt cattttgaaa
    14881 catccccttt tgtaaatttc tttaaaattt atatattaat tatattttca accaataatt
    14941 gtattttcaa ccaatctttc ttcatagcat tttaaatcat tttgctttag ataattaaat
    15001 tcagactcta cataaatacc acagcctacc aattcaattg tcaaattagg tatattttcc
    15061 tttagtatac tagtctgctc ataaataatt ctttccaata aatcaacttg taatatagaa
    15121 aaatgcttca taatctcccc atcactatct atatctttct tgatttcata ccaactcttt
    15181 gacaaaattc ccttcaaatc aatataccct agactcctca atatatctat actatcgctc
    15241 catgcatact tactaggttt atcaaagtat gattctagtt tcttcttctg ctcatttgta
    15301 agtgaatcat aagaaagctt cttattaaaa tgtgccttca aagaaatctt agccatagaa
    15361 tatggacttc taccagagtt taatcctgca tttttgaact tttcaattcc tttatcttct
    15421 actgaagacc atattgagtt tattgaatct gaatcattgt atttaatatc aagaccatgt
    15481 gtaatattta tatagttttg tggaaataca tattgataat agacatcttt tttatcatca
    15541 tatagagaga agtatccact atcatataaa tgaatatgca cctttacttt taaactatcc
    15601 tctaaaatat ttcgtctaag gttcgggata tttgcataca tggttacata ttttatggca
    15661 tcaaagtttt tatttaatgg aacttcaaaa ctaatttcat ctcctgtcaa aagctttttc
    15721 acttcttctt cattacaatc atatagagaa tatgtatttt caaatcccac acgtgatttc
    15781 ccatctaaac tgcctgttaa tgctaccttc aaaatatggt tatctgtttt ttgccattgg
    15841 taagcatctt ggtcaagaaa ctctctaatc tgtctatttt gctctttcgc ctgatttata
    15901 acctcgtttt catcttctgt aatccctaca tggcatctat ttaagatatt tactatattg
    15961 cttccaaatg ggaatgtcca tagttgaaaa gctgctgcct caacctgaca caaatatcta
    16021 acctgattta attcaatttg atggataagt tttttctgat attcattcat ctgattacca
    16081 ctatctgaca tttgataaat caattctccc attgcaagat tatctttttt cttatcaatc
    16141 ttatcagtaa tgataatttg agtttccatc atatcctgta cttttttaaa tattatagaa
    16201 atctcctctg agttttccat ttttaagctt ccaaacttcg tgcttaattc agttaaaact
    16261 tccttcatct ttaatttact tatctcattt tcaaaaatat cttgattttt aactgtacta
    16321 tcgaattttt ctacaaatgg tataacagat tctttcaaag ttttatcttt aaattccacc
    16381 atttgagtat tcaattcatc taatttctta atattatttt ctttatcttt aattgcctct
    16441 ttaactacct ctattttact ctcaaaacta gattttacat taagacaatt attaacacca
    16501 ctaatcgcag tatttataag agaacaagca gaacttccaa tactcaatgc atttttaatt
    16561 ttagatgtct tttgctgtct ttcaatagct tgtttacttt tctctagtgc agctaactta
    16621 tctttttgct gtttattatt gtaatttgcc tccaacatac ctatttcttt tttctggatt
    16681 tttgtgtaaa tttccttata ctcgctttca cttaacccct cttttgctct ttgaatatcg
    16741 tcagctttgt ctgcatagtt atcatcatct atagacttaa ctataatctc cttaatttgg
    16801 tcaaatttag ttcttctttc ttcatcaaca cctgccaaat tataagtcat tttatctaaa
    16861 gcattttttc tatcttcact tagctgttct ccaagtacaa atctcatacc atcagaatcc
    16921 aatttaaaat ctgaaaaatc aaccatcctg ttagtccatt ggtattcctc ttctttagct
    16981 gaaagatttt ccttcaaaga ttttgcactg tcaaatgccc ctagacaagc ttgaccaaca
    17041 ccactaaatg cagtcagtaa actcaatgcg ctttgtattc cagaaagaat taactgtgtt
    17101 ttcatttgtt cctcaagttt tgcctgtttc ttttttagtt cctctatgtc ttcttgttca
    17161 tcttctttca tctttataat ttcttgaatc aacaggttca tttttgcatt aacagaaagc
    17221 attttattct caagttcatt ttttaaaacc tctatttgtt gatttccaga ttcaatttga
    17281 gttaggatac tttccttata tgtattgaaa tatccttctt ttaactcatc tttctttcct
    17341 tgttccaatt ctcttaaggt cttatccaga tagtctgtca aagaacgaat atctgtaaca
    17401 cgatttcctc caatttcact ttcttcaatc atctgcacat aaaatgctgt cttctgctca
    17461 agtgcaaata aaatcatact ccattcgcta aaattctgat tttttcctac aaaagattct
    17521 attctatcag actgcatcaa tttatttata aggtattcat agatttcctt tgcctcttta
    17581 taaaattctt tctttatatt catgtcatcc atagaaagta ctgctttatt tttcttctca
    17641 aaactatagt acttctgaag tatttctcta tttaattgca tctctatttt ctcaactgca
    17701 taaaaaattt cataaatatg tgaaaattct aaaatatcag cttctaaaat tcctactgca
    17761 cacaacagtc taagatttct ataaccttta attccagaaa aaccaaatct ctcgaaaaat
    17821 tcatagaaga atgtatctat tgacaaagaa tactcctgct ttaacaattc cttttctttt
    17881 agtgcatctc cagagtgacc ggcttttccc tctgctccgg ctgaacctga atcttcacgt
    17941 ttgctaattg tccagtcctt atgttcttca taaagagtat ttaatttctc tccatcatct
    18001 gctgcttgct tctgatttaa agcatgatta aacacatatc tatcaaaata atagtagttt
    18061 ctatcactat tatccattgg aggagaggta cctatatatc ttattgtttc ttcatatata
    18121 cctctaattc caccaagacc tcctgcacca ccagctcctc cagagcctcc ttttcctgct
    18181 aaaccatgat ttccatcttt tttacttata gcacttttta ataaaggagc ttttccaagg
    18241 agataaacct gtccagactt gcttccaaga cctgcatttc caccattacc tcctgtgcct
    18301 ccagttccac cttgttttcc tttttctgct tttacaacat agtattttgt acctttaaca
    18361 ttagcaaatt cttttgactc ttttttagaa cctattttag catttgcccc agcttctcca
    18421 gttatccctg tatctccttt ttttccatct tgtccatttc caccacgtcc accatctacg
    18481 taaagtttaa gttctcctct tttttctatt tctttaaaag ataatatgat atcacctgaa
    18541 gtacctgcat tattgccatt tcttccattc attccgtcgt aaatagaact ttctccaacc
    18601 aatttttctg catccttacc agatacgttg attgaataag tcccttctgg tatatgtaat
    18661 ttttctgtaa gtagtacaat attcttacct gagaagactt ctttctccat attacagtca
    18721 attttcaact cacaatctcc atacatatat atagatgaca aaatattaag ttgataacca
    18781 gatattattt tagtcaaatc cgataaattc actttaggta taggaatata tatagaaagt
    18841 acattttctt tctcatatga ttttatatat gtacaacttt gaatcttctc tatcctatac
    18901 tctgcatctt caagcttttt ggcttcttct tccctatatt cttcttctgc tatccaatat
    18961 ttgatatctt ttaatgtatc ttctaaagta ttttttagca atgattttaa caactcactt
    19021 agaccaacaa tatgagtctc actacagtaa ctatttaact ctaaaagaag acgtgtttct
    19081 tctaatatat tcactttctg tacgccatca ccatatgaaa gttcttgtac aaactccata
    19141 agctctttca tattattagt cctactacat aaatctgtat acatcttatc taattgaatc
    19201 tttttatctt ttagactatt tatagaagat agaatggaaa tagaatcttc catacctttt
    19261 ttaacacttt ctatataagg aactaaaagt ttttctaggt ctttataggt tacatcaaca
    19321 gctttttgta tttctatttt acaagaagct gtaagcatca atcccaaatc tgttttttca
    19381 aatgtttcag gtagattttt catttcatcc aacaactttt ctggctgttt atctagtgga
    19441 ttacataatt taatagatac accatctgat aatgctacat ttaataagta cactaattcc
    19501 ttacaaccgt cgtaatcttt ctcttgacta tctaagtctt cttttgcttt ttccaatata
    19561 tttcttattt tttcttctgt tacattaccg attagtgttt taggtatttt attaataaga
    19621 aagaagaaat tttctttata ctttgcataa tcaccaagca tatctttcaa atttttgcct
    19681 gtttctatca ataatgtatg tctagagtcc agtagggatt gatagtctaa gataactacc
    19741 atccctttca cctttgaaga tgtggaaata gtttccctta caagcatagc accagcaatc
    19801 atatactctg ctccacggtt gtcaaaaaaa cctgggcaat cacataatga aaaatctcta
    19861 tttgcaatat ctacaacttc tggacataaa gtcttagaat atacaccatt tccaatttcg
    19921 acagctgaat cttttgcagt tatatatatt tgtccagtct cttcatccgt tttttcttcc
    19981 atcacacagc cttttaaata atttatacaa gtactctttc caacgcctga agcacctagg
    20041 aataacaaaa ttgacttttc ttttaagtca tattttctaa gctcgtctct tccttcttcc
    20101 aaaaggttta cgatttcact ataaagttta tttgccatac atccatccct ttctatattt
    20161 cggaacaata gccttgatat ttttctttaa taagattctc ttctaccttt gaaacagctg
    20221 ttgataactt agtatttgga gcattaaatc ctccattgtc tcctctatct ccatattcag
    20281 cgtattctct tgctggtcct gttttacctt cattccagct tccaccacta aattctatat
    20341 gatatttaat tatactatct aacttataat cccaacttgt aaaatatcct gtatagtata
    20401 cagtcttatc tataccactt ggagaaaatg aaagtgtatt atatccgtga tagccaccac
    20461 ttcctccata tccacctaaa cctggtctac catttggacc tgcttctcca tctttcactg
    20521 ttatatcaca atgattaatt attctatttt tttgttcctc tgaatctgaa atgaagtaaa
    20581 tattaccttt cttaccagca tttccacctc ttccagcatt tccaccagct ccaccttttt
    20641 cacctttttt gccacttgta cctttttcaa aatacccttt aaacatctgt ccgtggaaat
    20701 aacttgtata tcctgcatca gctccattac ttccagaagc tcctgtttca ccattttctc
    20761 catctattcc tttagaacca tttcctccat ttgcaatcag tgttagacga taatttctaa
    20821 tttcttcttt aatatataag taaatattac ctgcatctct accatcttca ccatttttcc
    20881 catacataga attatctaca agttctccgc tctttccact tacattaatt tttacattac
    20941 tacctacatc aagattgtta gaagcaagaa taatattttt ctctgatgca aagttcattc
    21001 caaggttatc atccatataa atagtatctt tagcaaagaa cataaaagta gaaacattgt
    21061 catctacttt tctatttttg aaatattcca atacactgct aattactaag tttggaatta
    21121 atgctcttat ttcaatacct ttcttatcat taatattaat tgtttttact ataaatggat
    21181 ttaaaatgta tttaccaata aattctctaa ttatctcatt accttcttct ccatatgcac
    21241 ttaatcttgt tatactgtct tctaactctt cattttgtgt aactgtaaca tttgtcaaaa
    21301 ttgaattaaa atcacttaca ctatatacat aatccttttt atttgcaact tccattaatt
    21361 tttctctatt tggtaatgta aatacctgtt tttgcacttc ataagaaact aaaacttttt
    21421 ctaaattctt tcttattata atgaagtttt gttccattat cttcttactt gccttttcta
    21481 taataaattc catatgtaca gaaggtaata aaatttcccc agttccatgt tctgtatatt
    21541 tacttaaagc atcaattata tctaagtttc cttttatctt attttgtaat ttcatgcaac
    21601 tatcttgtaa gaataatcca ctttgtttgg ctaatacgtt gataaaactc tctatagtaa
    21661 gacttgttgt actttccaac cagtttttaa atacttttac aacctcatca tattttgcta
    21721 tttcttgaat cattgttata cttccaataa tattgataaa atcttctgta tacgccttaa
    21781 cataatcttc taggctagat acgatattgt tttgaatttc ttttatcaac gtatctaaaa
    21841 ctaatcttgc atctgcacta attggatatt ggaatgctga tgtcttatct ttaacttcat
    21901 ctaattctag tacttgcttt aataagcctt ccctctcatc tgatgcaagt ggcttacaaa
    21961 gatgaatatt ttttccatta tcttttagca attcttcaaa taaccaagct ttttcctcta
    22021 tattactgtc taaattttcg cataattgtt catattttct ttttataaca ttatatattt
    22081 gtttttctgt tcttcctaag aaactttttg aaatgaaaaa agttatacta cccttaaaat
    22141 tttctttatg aagacacata tttaactgtt ggaacaggtc tagtactttt gtaagtctag
    22201 aatctaatat atctgcttct ggtatcaata caattactgc acatatagat gcagaatttt
    22261 taaatacttc acctaatgaa actgctgcac taatatcatg ggctactccc ctattgtcgc
    22321 taaaacctgc agtatcacat aattttgcat ccattccttc tattggtttt ctcatattta
    22381 caatgttcgg gtataaagtt tgagaaacag ctcctgaacc aatctctgcc acagggtcta
    22441 agcaatcaat atatgatata ccagtatctt catcttctac ttccatcatt acacatcctg
    22501 caagatagtt aattgcagta cttttgccac atcctgtccc accaagaatt agaattaaat
    22561 tttgattttc agccttattt attgctactt ttccttcatt taataaaagt gttatttctt
    22621 ctaaaagttg attgttattt tctggcattt attttccccc ttggttatat attttattta
    22681 aataaagttt aatcccttaa gtagattgca aagtttttag actatattta aatttgtttt
    22741 ttatttttta tttttaataa atttgcattt tcactaaata attattcttc tttgtttatt
    22801 aaaataactg tgttgtataa tataatcttt atcaacacag ttattttatt taatgcgctt
    22861 attttattgt attttatgta atacagttgt tttttatatt atatgtccat tatttttcta
    22921 tggttattac atatagattt ttaattatat ttgtaccatt aatttaaaca ttcctatact
    22981 actgcttaag ttttacacct ataccagctt gaatatttta tttttatttg cttaaatagt
    23041 tcaactatac tggtttaaat aggttatctt tatttgctta aatagttcaa ctatactggt
    23101 taaataggtt atctttattt gcttaaatag ttcaactata ctgatttaaa taggttatct
    23161 ttatttgctt aaataagttc aactatactg atttaaatag gtttccttta gtttaaataa
    23221 ttcacctata ctggtttaaa taggtttcct ttagtttaaa taattcacct atacctactt
    23281 gaatagttca cttttatttg cttaaattct ataccaatac tggcttaaac attatactac
    23341 catgattgta ttgtctatct tcaactacaa taaaaacagg ttctaaatct ttagtagatg
    23401 ttatttttaa ttctaaaaaa atattatcag ttggtaatat ttttacatca taattattta
    23461 aattattatt cgtgatacta ttaaatacat caccttgacg ttcaaaaaaa acattatcaa
    23521 ctattacaaa ataaacatct tcttttctac tattttcaac ctctatagta ttttgtttag
    23581 ttatatttag tctcatcttc cacctcctat taatattact taattatatt aatacttgta
    23641 attgttgcat tatgttcatc acaaggaaga ggtttaaaaa caactatctc tgctaaataa
    23701 ttctgtggag gatttgtaac tgaagtagca ttgcaaataa atttaaaatt tgttcctgct
    23761 acaacttgag tagaaacaat aagtggttca taaccaaccc cttctaacat tcctacagct
    23821 tcattaaata cttttaaatc ttcttcttcg acagctctga attttgacca tccaccaaca
    23881 aaaagactca tataaaaacc tcctttatat atatatttat ttatatatag tagtaccaat
    23941 tttctcaata ttcaatattt tctgtgtaac tggtcaatga aaatgcataa ctggataaaa
    24001 atacttataa aataacttaa ctttacatat aaatagctta ctttacatgt aataagatta
    24061 ttctacaata tgaaatgcat cttgttttga atatattcta aatacaataa tggatatttt
    24121 caattattat aattagaaaa tatccattac ttaacttgtt ttatttaaaa tcacatagag
    24181 actttattat tttttttatt gcatgaatag ctatttcagg tatcataagg tgatataccc
    24241 cctccttgaa accacaaaaa acatgacaaa ctttacgaat cttgtcttaa aaaactacag
    24301 gtacattcct tatttgtgga ttggagtagc cataacaagc aaattttaca atttttatga
    24361 tgacaaaaat caataaaatc gcttagtata taacacgaaa tactacttgt tactattaag
    24421 aatagttaga taggaagtat gcattataca aataaaggaa tcaataaaga aagttaaaac
    24481 atccctacaa attaaaaatc tgttacattt ttctatattt aaacgttatt tacttacaat
    24541 tggagaaata acaggttttc cttctcggtc aagtaaaggc gtaaatccgg ctgcattgcc
    24601 ataatagtgg aatacatagt tcacacctgt ttctttatcc acccaaatct ccgttgcatt
    24661 tattttccct tgtgaatata ctttttcaaa tctatctttt cccattttat aatcctccat
    24721 atttttgatt ggtctatgcc ttcttcactt aagtagaaaa aatacataac ttattttttt
    24781 aattctaata tgagcacgta aaaatgtcaa acagcttatt taatttcata acttcatgtt
    24841 actgttcgta accttgtctg tttacttaca ctgcattaca gcaccatttt atcctttaca
    24901 ttattgtatt gcacacagta aaaatgctta ctttatggga tttttcaact aaaaaaagga
    24961 ttttatctcc acaaaacatt tactatttgt tacaatatac ctaattttaa tctcatatca
    25021 acatttaatg aagatataac ctttttcaaa tattgtaaag actccttaaa aatattagaa
    25081 aaagattctg caaatctctc aacatctcct ttttttaaat acctaagtat ctcaaaacat
    25141 acatcaaaac gtgttttcaa aatttcatta tattcttcat gaaaaaatct ctctaaatag
    25201 attccaaata tcatatattg ttttaaatgc aaaaatgttt cctgtaatac ataaatttgt
    25261 gtataatcta ctatcttaca aagtaaaaca aaatctggat aaaccttaca ctcttcacaa
    25321 ttattttgta agtaccttaa atgagtttct attttatcta cagctatcaa atccatttta
    25381 tttcctagta aaagtactac atcctcaaga gtaatcgtaa caaactccat agcatccaaa
    25441 agcaaactca accttttagc tccttcttct gtatttatat aatcatcatc atattcagaa
    25501 aaaagcgtta catatgttcc cttgccatta attgttttag caattcccaa ttcatttagc
    25561 tcattatagg cacttctaac tgttggaagt gatacattat atctattaat tatcgaagta
    25621 attgatggta gacaatctcc tattttaata ttgccttcaa aaatttgaca aattacatct
    25681 gaaacaatta agtcatataa ataacatttt tccaacgtta cagaattaaa actacttgta
    25741 tctatacttt gtggagcacg attttctacc attttaagta tggattctct acaagcttta
    25801 tacatatatg ttaatagttt tttaaattca attagattcc catccaatcc atataagtaa
    25861 gtttttctta cctgctctat gtatttatta cgaaaaaagt ctatacaaca attatccaag
    25921 ttatccaagt tatcgaaatt ataggccaat tgtgaaataa tgacatcatg ctgaataatc
    25981 tcacaaaaat ggtgaatcag gaaattattt gttttactta ttagtttatt tatatatttt
    26041 agcctttgtt ctttaaagac taaataatta gaactatttt tttccataca atcgataatc
    26101 tcgtttaatt cttcaaagtc tttagatgta aatcttttaa tattgtacat agcaatagaa
    26161 ggaaatatta aactaaaaat cttatatatt tctgatattt gtaaaatctt ctcatataat
    26221 aagtcagatt ctattgattg tttactcgta ctctggatta aaactggtct agaaccagct
    26281 tttgtctcta taaagccctt ttctgagagc atttttatta cagtttttat agtatttttt
    26341 gataccatat attcttctga catgcttttt atagaatcaa gcctatctcc aatagtaatt
    26401 ttaccagtat aaatttgttg aattatatgc tcaaaaataa ttaaatattg tggttttctc
    26461 ttcatatttt tcctcttcga taaattagat ttattgtttt ttgtaaggta tatttacttt
    26521 tatttaatag ggcaaaatag ttgcttattt tttcctcata atctataatt aatataaacc
    26581 atttatatat atggtctaag ttttataata ctgtaatgca gtaaaatagc atgttgtagc
    26641 tatactacag caaattaaca taccattgta gtatattaat tggaataatt ttaagtactt
    26701 caaaattcca gcaaaacata tatgtgtttt tgatattatg tatattcgtt aatagaatag
    26761 ctatacttgt tttacataat aaattatatg atacttgcta tatgtaatca ctatatttaa
    26821 tacacataac tattatactt gctatatgta attactatac tttatacaca tagttatcat
    26881 atatgctata tgtaattact atactttata cacatagtta tcatatatac tatatataat
    26941 tactatactt ggacacatag ttatcataca tgctatatgt aattactaca cttggacaca
    27001 taactactat atttactata tgtaattata ctatacttgg acatatagtt atcatacata
    27061 ctatatgtaa ttactatact tgaacacata actattatat ttgctatatg taattatact
    27121 atacttggac atatagttat catacatact atatgtaatt actatactta tacacataat
    27181 tatcatacat gctatatgta attactatac ttatacacat aactatcata catgctatat
    27241 gtaattacta tacttataca cataactatt atactttcca tacataatta ctatatttgg
    27301 acacatagtt atcatacata ctgtatatta agatataata ctttttaaat tttatataac
    27361 acagacacat gcaataaaaa agaaggtatc cataataagc tctttgcatg tacctaaata
    27421 aaaactgtaa aatagaatat ataaaatcta atctatttta agtaaataca atttcacttt
    27481 aaaagaggat gtactatgaa ataccatata tttttaatca taactactat attaataatc
    27541 gaactatgta acatttacat atgcaaaata gcatcttttg gaaacaaaaa tataactata
    27601 cataagaatt acaaatatat ttttttaata aaaccattaa ccttcttgat aataatattc
    27661 acaaaattac cattaccact atattatata ttgttttaca tagtaagtca taaattgctc
    27721 ttaagcagaa aaagttctac taacaaccat tttattccca atatggcagt aataagtttt
    27781 tgtattttac accttattac tgtaagtaca atatcacttg ttaaaaatac aagtccagat
    27841 ataatatttg caaatatgac ctataagtta attagtattt taatagcttc tattttagat
    27901 tttatcttat ggtactctat gaataaaata aaagagcata tttatataat aactcaaaat
    27961 caaatagagt tcagtctatt tttaaaattt atctggttca ctttaggata tatattattt
    28021 gatagtatac aaattacatt tgctccacca agttctctaa cttcctactt tatattggga
    28081 agtaacatat tattaatctt acaactaatt atattcatca atcatatata ttccattgaa
    28141 aaaaatattt atttagaaaa agaacataat agactaaaaa tgataaaaca agaacaatta
    28201 ctaaaagtaa atttattaaa gaaaaaagct aacattgatg aattaacatc agcatataat
    28261 cgaaaatttg cttttgaaaa aatacagcat tacattgata acaatttatt attttcattg
    28321 atatatatcg atttggataa attaaagacg ataaatgata catatggaca tttagctggg
    28381 gataactata ttattaactt ctcaagtata gttattgaaa acttaggaat agatgacata
    28441 tttgctagaa taggaggaga tgagtttata gttattatgc ctaaatctaa tgaaaacacc
    28501 acaaaagaat ttatagagaa aataagtaaa attatggata atacgatatc ttttagctat
    28561 ggtattgtag aagtttctag cgaaaacaaa atgactgtag aagaaattat taatattgct
    28621 gatattagaa tgtacactaa taaacaaaat aatcataaaa ggaacaataa ctaaaatgaa
    28681 tatttacatg agcttacttg tagtctcact atgtttttta tcttattatc tctatcatta
    28741 taattttttt aaatatctgg acataatgat taataactct aaaaataaat acacagtata
    28801 tattatatat tctatattta attatttaat ttttattatt tgtacttatt taaagtttca
    28861 tctaattata aattggacaa tttttatgtt gattttattc atacaaatta aagtaactta
    28921 taaaatctcc acaataaaaa acatttttta ttcactaaac tgctccatca ttggtttagc
    28981 aataaatata ttttttaggt ctttaatgtc aatagtgtta aatatgccag catatttatt
    29041 tgatagtaat tctattgcaa atatttacag agtatttcct atatcattta gctttatagt
    29101 tacaggacta tatttcagtt ataaaaataa taaaaataat aagattaact ttgaggcagt
    29161 tttagaggac aaaaaaaact taattttctt attgagcctt atgtttggaa tgtacttgta
    29221 tctatgtttg agtttgtcaa tatactatac tgttggaaat gtattgattt taaaattata
    29281 tggaatcaag tctgccataa tagttttggt aggtaactat attgcagaaa gatatagctt
    29341 tagaattact caaataaaag gttatgatat taaaaatcaa atagaatatg tagaaagtgt
    29401 aaagacagaa tatgagatta ataaattgaa tgatattgta tatacagatt tactgacagg
    29461 tttctacaat agaccttttg tgaatcaaaa actatcagaa tggacatcaa atcactacaa
    29521 atttacctta tgttttactg atttaaatag cttaaaacct gtaaatgata aatttggaca
    29581 ccaatttggt gataaataca tatccactac tgctcaaata ataaaaaaac ttataaatgt
    29641 agaaaattcc ttactattta gatatggtgg agatgaattt ttattccttt tacagaatac
    29701 aagttgtgta gaagcagaaa agataatgat taatataaat tatgaattat caaaattaag
    29761 taatactgac gagtatcctt atgctctttc cataagctat ggattagttg agtgggatgg
    29821 aatatccgat attgaaactt taatatcaaa ggcagattct ttgatgtatg aaaataagct
    29881 taaaaataaa tctactttaa actagctagt taagatattc tacaaaatat atgttataag
    29941 agagtactaa gaatatcaaa atatatagat tttgcaaata ttgaatacag tttactaatg
    30001 aatgcactgg atgtaagtgt tagtaaacac ctattagatg aacactttac tgttgtttgg
    30061 gctaatgacc gttattatga aatgttcagc tatacctagc taaaaagtta aaaatgacca
    30121 ctgtagccga gggaatagag accagttttc aaaaagattt cttgcaagaa ataaactgtg
    30181 atatgttaca aggatacgtg ttctctagac ctttaccaat aaaagacttt gaaaaactta
    30241 tgtttcaaaa ctcctccaat taacaatata aaattgctta ttataagtga tattattgta
    30301 tcaacaaata aaggaattgt ctcaaaattg attttttcta atcaaatttt tattttgaga
    30361 cattctcatt tttttgcata agaaaatacc caccaagcta cggtttttct taccgctgtt
    30421 cggtgggtat tagtagtatt atgaaatctg aaaactttaa gtattactac ttccgctaac
    30481 gcaatcacaa aggtcgaaag cttcaaagcg aaaataggct ttttactcat caaacgtcac
    30541 cgtacctgcc aacccgtcca ccgttatcat ttgtccgctc ttaatagttc gtgtggcaat
    30601 gtgtgtagcc ataacggctg ggatgccata ttcacgtgcg acaatgcttg aatggctaag
    30661 aggaccacca atgtccgtta caatcgcact tgcagatgaa aataatggtg tccatgctgg
    30721 agttgtggta actgcaacca aaacacttcc aggttgaaag ctttcaaaat ctgctggact
    30781 attcagtaca caagctggag ccgtcacaac accagtactg gtaccaatgc ccgtcaacac
    30841 aattttccca tctttctgct tctgttgtgc ttgtgatata gtttttttgt ttttctctgg
    30901 caacttggat ggtgacatat aacccatgta ttttttaagt tcagcttttc gaacaggtat
    30961 tatcccgttc atatcagata gagacttgtt tttatccaac tgctctataa gtacctccaa
    31021 ttccgatttt gtgagccaat aaatatcatc taaatgtgaa atagctcctc ctcgtatgaa
    31081 tcgttctgag atttcctgaa acatacaacg aatcagagga tgtcccattc ccatcaaata
    31141 aatagcattt tcacgcatag gagaggtttc ctgtgcccaa tggagcaact taaaaaatag
    31201 ttttttacgc aatccaccta tgtgttgtaa aatctcttct tctgcatgtt ttctacgctt
    31261 ttcaaacgtg gtctggcgta aaaaaggact ttcccctttt ccttccacaa atgtttttac
    31321 ggattcaaaa gttggtgtaa gtgtttcctg cggtgtggaa tgcgcaaaat caaactcata
    31381 agcggtacga ccaaactctt taaagtactt attgattcga tttttccatt ctatccatac
    31441 ttcctgtgaa actccaacag gcacaactga agacatgaaa tcttctgcta tctttattgc
    31501 aggactgttt tgcagataaa ggttgatgct gttattttgc tttacccatt ctgaaatacc
    31561 ccacaactcc ttttccgatt gcagaacaat cgtatcaaac ccaagcagga aaacggaagt
    31621 atccgttata ccagcacgtc gaactgctcc ttgaaataat tttgtaaata atgtttcact
    31681 gatagaagca gcaggcaatg taagctgaat ccttgtgaaa taaatacatg cggcataaaa
    31741 tattgtctga ataccctcca taatttgatg ggcgttcata gtatgcaggg gcttttcttc
    31801 ccattgttta atagcatcct catatccttt tcgtgccgtt tcccagcgtg caacgctgtt
    31861 cataagtgtc ctacgtaaag agcgtgggga aagggatttg atagcaatca aaagtggctt
    31921 tgatttagca gaaagataaa catatccatt aataaccgta tatgccccgt tttttggtaa
    31981 taattttttc gcacttttac caaacatatc tatccataaa agctctgatg cacggtttac
    32041 gatttcaaga ccaagtgtgg cgaacaaagg tgtaacagga tttggcaaat gttctgccaa
    32101 acttcccttt gtgtatacaa catcctgttc tggcaatacc cattctggtg gcagaacagt
    32161 aattggacga gcctgaacaa tgtacaattt atctttttcc agtgcccact ctacatccat
    32221 aggcatttga taatacttct caattttctt tccaagctgt atcagttgca ttacctgatt
    32281 acgagtaaga gcatattttt ttctcaacct ttcaggaacc atggtttctt ctgttccttc
    32341 tgaagtacga accgtcataa tctctttatt tgccacttca tacgatataa ttcgttcgga
    32401 gtccttatcc actacgattg tatcgggtgt aactaagcta gaaaccaccg cttcaccaag
    32461 cccccatgca gcgttaataa tcatttcgct acgtctacca ttgattgggt ttaacgtaaa
    32521 cataatacca gatgaatcgg aaaatgcaag cttttgtact acaacagcaa gtgcgacaat
    32581 ctcgtgcttt atattattct tcacacgata agcaatagca cgagctgtcc ataaggaagc
    32641 ccagcatctc tttacagagt caataacttt atcttcacct tgtatgttaa gataagtttc
    32701 ctgctgacct gcaaaagaag cgtcaggtaa atcttcagca gtcgcagacg aacggacagc
    32761 caccgatatg ttccctaatc cagcataagc cattttaatt gcgtccgaca cttcctgtgg
    32821 catttctcca ttatggaaaa gctcccctat tttcttagat acatcttcaa gttggctggt
    32881 attattggaa tcaataccat ctagcagttt attaattcga gactgaatat ggttcgtctc
    32941 aacaaaaatc tggtatgaag cagtcgtaac atgaaaaccg tctggcacag ggatgcctgc
    33001 tgtcaatagt tttgataaag acatcccttt tccaccaaca atttcaatgg ttgcttgttt
    33061 atgttccagt ggcaagacat aataaatcat aatcattcac ctccatcatt cataattccg
    33121 ttcagaaata tattcattgt ttcatccaac attttttccg aactatccaa tggtcgtgac
    33181 gtgtagctga ttgataacat ggagttaatt aacaaacgtg tcacaagagc ggtatccgtt
    33241 ttgcgaaaga tgcctgtggt cacaccatta tcaattaccg attttataat atcttgatat
    33301 gcataacgag catctttaag tctctttcga tactcttctt ctaaataatc agattctgta
    33361 tttaaccaca tcagtacagt tctatactgc tctggaaatc taagatgatt aagcatgatt
    33421 tttctaaggc actgttgtgg tgaaggttca ccatcaataa tcctatgaac ttgttggata
    33481 gttttttcta tttctttctc aactgcatat acaacaatct cgtctttcgt tttaaaaaaa
    33541 tcgtatagac ttgactttcc catgttggct aaaactgcaa tttctctcat tgatgttttt
    33601 tggaatccat tttcagccat gagactcaaa gcaacagcaa ctattttttc tcggcgttct
    33661 gcttgttgtt cgggtgtaag cacaattcct tttggcatag tgcgatacct ccttttagcg
    33721 accactggtc gtttcttttt tatttatcat acagcaacca acggtcactt gtcaaggtta
    33781 atttttttca ttgtatatgg tactcgagat aaaaatgttc attttagaaa ttactctatt
    33841 taagtagaaa taggcttcat cattagaaag caagactata aatttgagat atttatcatg
    33901 ttcaaatttc atcaatatca aagtttaaaa tattaataaa aaaagaatta ctaagaaata
    33961 acatataaag attcaaaaaa ggagttgaac ttaaaattca attacaaaaa atcaaattta
    34021 agacaactcc tttattcttt atctctacta ttctttatct ctatttattt attacaactc
    34081 cctaacattt accactactg ccttaggtct agtcatagcc tcaattgaat acttaacacc
    34141 ttgagttccc ataccagatg cctttacacc taaaaatggg aagtggtctg gacctctttc
    34201 agtcttatta tttatttgaa cagtaccaac ttcaagttta tcagctatat aaaatgcctt
    34261 atcaatatca cttgtaaata ctgatgattg aagcccatac tctgattgat tagcaatttc
    34321 aattgcttga tttatatctt taactcttat aattggaagt actggaccaa atggttcttc
    34381 ccaagcaatt ttcatatcaa catttacatt atctaacaat gttggataga ataaattatt
    34441 ttttctaaca ttgcctgtta ttaactttgc tcctttatgt aatgcatcat caaccaaccc
    34501 ttgaacaaaa tcagttgctt tattatctat aagaggagtg ataactactt catccattgg
    34561 atttccaatt gtcaacttct ctaccaatgg ctttattttt ccgactaatt tatccgccac
    34621 actctcttgt actaaaattc tctttactgc cgtacatctt tgtcctgaat atgaatacgc
    34681 tccagatact atattcttag ctgcaaaatc taagtctgca tcttctaata ctatagccgc
    34741 atcttttcca ccaagctcca ataataatgg taccattcca gatattttag atatatgctg
    34801 accaacttca gtactaccag taaagttaat aaaattaatt cctttatgcg ttacaacata
    34861 atcccctatc tcactacccc tacctgtaat agtattaagt actccatctg gtatcccagc
    34921 ttttctcata atttctacca catgaagtgc gctaatagcc ccttgtgtag caggttttaa
    34981 tacgactgca tttccaccaa taagtgctgg agcaatcttt gacattgata aatttacagg
    35041 ataattaaaa ggagatatag ctaaaacagt acctaatgga acccttgaaa cataagacat
    35101 cttatttctt gttccaccag ggaaattatc tccactaatg gcttctccct ccatattttt
    35161 tcccacatca gctgtgtatc ttaaaaaatc tgctgttctc tcaacttctg cccttgcaga
    35221 ttttatgtct ttggctattt caagtaccag tatattagct atctcatcta tattttctaa
    35281 caataaatca gcagccttat gaaatatatt gcctctcttg tatacaggca tttcagccca
    35341 tacttttata ctctcttttg tgttttttat aacctcatct acctcatgtt ttgtcataga
    35401 ttgtactttc ccaacaagag aattatctat tggtgagtaa atttctattg gctttctcga
    35461 attagattct acccattttc catttattaa atttttatat acattttctt ttgttttaag
    35521 ttcattaaac atatttttat tcctctctct gatataactt tgtgtattta aattatagcc
    35581 atttattcgt tatgtaaaca tttctttttt tcataaacgt ttacctataa aaaaatttta
    35641 agttaacgtt tttctacatt cttctatttt aataacatac tcgctccaaa tctatatagg
    35701 tcacacttta atactaatat atcaatatac caatatacca atataccaat ataccaatat
    35761 accaatatac caatatacca atataaatat tataaacata aatcacttga caaatttata
    35821 agtagacaat attatatatc tatgactcaa aatattttta aagtcatata tatcatatat
    35881 agacagaaag gattgataaa aatggctaaa agttttacag aaatagaaaa agacaatata
    35941 agaaaaagac taatttcaga atgtgaaatc aactggtcta aatatggata caaaaaaact
    36001 aatattgatg aactttgttc aaaatcagga attgcaaagg gttctttcta tacatttttt
    36061 gattcaaaag aaaagttatt ttttgaagta ttaaatggaa tccaaaacag acttatatca
    36121 tcagtagatg aaattttaag taacatgcct ccaaaagaag gctttgcaaa atccttaaaa
    36181 atgttatatc gattatatga taaaaatcca tttttatatt caccaaataa tcctgatttc
    36241 atcagcctaa taaataaact acctcaagat atgtttttac aacttgagtc caataacata
    36301 caaagttttt acgacttaat tgagagacac aatctcaagt tgaagataga aaaagaaaaa
    36361 gtttttggtg tttgtaatgc tctactatca agtctgttgg taaaagaaaa tattgaatat
    36421 aactatattg aagtgtttga ctttatggtg gataatcttg tagaacacat acttgaataa
    36481 caatttaaaa taatttttct aagtattttt ttatactaaa taggattaat atgattgaat
    36541 taatgcaaaa aataaacaat ataatttata aaataaatga taaactaaat atcacacaaa
    36601 acttatagac cctagagcaa acccattcca ctctactcta agccatctat aatagtttgt
    36661 atatttctaa taatattaga tattaaagta agaaaaaaat tccttataat aattgctatt
    36721 atatcaatta ttgtaaatgt tttaataatc ttagataggt aggtacatat atggtaaaat
    36781 cctcgaacac ttttaataaa aacttcaact tgatgatatt aggtcaaata atctcattgt
    36841 ttggtgcatc aatacttaaa tttgctttat ctttatacat acttgatata actggtaaag
    36901 cagaaatatt tgctactata ctagcagtat cttcaatccc aattatagta ttctccccta
    36961 ttggtggagc tattgcagat atattcaaca gaagaaactt aatggttatt tttgattttt
    37021 caagtagtct tacagtacta atcttagcag tattcttatt caacaataat ggttcaatat
    37081 tacttgtagg aattattatg accatattat cagtcataag cacaatgtat caaccaactg
    37141 ttcagtcaag tacacccctt ttagttgata atgagcattt aatgaatgca aatggtattg
    37201 tagctggagt tgcatctctt acaaatattg caggacctgt attaggaggt gtcttgtatg
    37261 gtgtgattgg aattaatgca attatagtta taagttgtgt aagtttcttt ctatctgcaa
    37321 ttatggagat atttatacag attcctttta tcaaacaaga gcaaaacagc aacattataa
    37381 aaactatatt tactgatata aaggatggta ttgttcatat atgtaaagaa aatagattta
    37441 tactaaaaat acttttttta gctatagcaa taaacttatt tttaagctct atgttaatag
    37501 ttggtatccc ctatatagta aaaatcacta tgggagctag taacttctta tatggtctag
    37561 cagaaggagc aatttctttt agttcactgg tagctgctgt tagtattggt attttctcaa
    37621 aacatctaaa aatcactaat ttgcatattt cctttattat atgtgcagtt atgttcatac
    37681 caatggcggt ttcagtatat ccatttatat taaacacagg atttccagtt ccatttttaa
    37741 tctttatagt atcatctatg ctaatatttt ttacaacgac tcttatctca atatatgtta
    37801 taacagtcac acaattaaaa actccaaatg aactcttagg aaaagtaatg tcaattcttt
    37861 ttgcaggaac agcaattgca atgccccttg gtcaggttat gtatggtaaa ctttttgatt
    37921 tattatcaga tagagtgtac atcattatac taggagttgg atttattaca ttacttatag
    37981 catttgtatc aaagataaca ttgaggaacg aaaatcttta gatacaaaga atttagatat
    38041 aaagatttaa ttttcactaa tttctgaatt taaatgcaaa attgtgtatc agtatgttga
    38101 cgttttagaa attgattgat ataatcaatg gaaaataaac tttataaaaa ttcaaaatat
    38161 tcagctagag gagtgataaa aatgagttac atgccaactt tagaacaagc atgggaaata
    38221 ttaagaaaat acaataagga agaatttcat ataagacatg ctcaaattgt atctggagtt
    38281 atgagatact atgcaaagga gtacgaccct gaaagagtag aattttggga aatagttgga
    38341 cttttacatg atttagattt tgaaatgtat cctgatgaac attgtgttaa acaaagagaa
    38401 ttaatgacag agcttgattt agataaatca ataatcgatt ctacaataag tcatggatat
    38461 ggattaactg gttcagatgt taaaccagag caatttatgg agaaagtatt atttgctgtg
    38521 gatgaactta ctggtttaat tggtgctgct gcaataatgc gtccttcaaa gagcgtttct
    38581 gatttagaat taaaatctgt taagaagaaa tttaaagata aatcatttgc tgctggctgt
    38641 tctcgagacg ttataagaag tggtgcagaa atgcttggat gggaacttga taagcttatc
    38701 caaacaacta tagaagctat gagaagcttg attccagaga tggcaatata gctcctccat
    38761 acatatacca tagaagttac atctttaaag agcatttctc tttttgatgc aacttctttt
    38821 tatattttgt agaataaaaa gtttaatatt ttaaactcac tatataataa ataatataat
    38881 gtataatagt cataaacttt ctaaccttaa aagtttctaa taatcaataa a
//
LOCUS       DAMJYX010000025        38420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJYX010000025 DAMJYX010000000
VERSION     DAMJYX010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.025"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            51..491
                     /locus_tag="PC479_001443"
     CDS             51..491
                     /locus_tag="PC479_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2803992.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            507..1958
                     /locus_tag="PC479_001444"
     CDS             507..1958
                     /locus_tag="PC479_001444"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2803993.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(2049..2717)
                     /locus_tag="PC479_001445"
     CDS             complement(2049..2717)
                     /locus_tag="PC479_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2803994.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            2986..3126
                     /locus_tag="PC479_001446"
     CDS             2986..3126
                     /locus_tag="PC479_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2803995.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(3301..3621)
                     /locus_tag="PC479_001447"
     CDS             complement(3301..3621)
                     /locus_tag="PC479_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2803996.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(3741..4478)
                     /locus_tag="PC479_001448"
     CDS             complement(3741..4478)
                     /locus_tag="PC479_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2803997.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            4693..6516
                     /gene="acd"
                     /locus_tag="PC479_001449"
     CDS             4693..6516
                     /gene="acd"
                     /locus_tag="PC479_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2803998.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            6731..11029
                     /locus_tag="PC479_001450"
     CDS             6731..11029
                     /locus_tag="PC479_001450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2803999.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            11233..11694
                     /locus_tag="PC479_001451"
     CDS             11233..11694
                     /locus_tag="PC479_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2804000.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            11724..12878
                     /gene="nusA"
                     /locus_tag="PC479_001452"
     CDS             11724..12878
                     /gene="nusA"
                     /locus_tag="PC479_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2804001.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            12885..13166
                     /locus_tag="PC479_001453"
     CDS             12885..13166
                     /locus_tag="PC479_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2804002.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            13156..13467
                     /locus_tag="PC479_001454"
     CDS             13156..13467
                     /locus_tag="PC479_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2804003.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            13489..15429
                     /gene="infB"
                     /locus_tag="PC479_001455"
     CDS             13489..15429
                     /gene="infB"
                     /locus_tag="PC479_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2804004.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            15472..15852
                     /gene="rbfA"
                     /locus_tag="PC479_001456"
     CDS             15472..15852
                     /gene="rbfA"
                     /locus_tag="PC479_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2804005.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            15833..16816
                     /locus_tag="PC479_001457"
     CDS             15833..16816
                     /locus_tag="PC479_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF2804006.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            16829..17734
                     /gene="truB"
                     /locus_tag="PC479_001458"
     CDS             16829..17734
                     /gene="truB"
                     /locus_tag="PC479_001458"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2804007.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            17865..18794
                     /locus_tag="PC479_001459"
     CDS             17865..18794
                     /locus_tag="PC479_001459"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2804008.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            18898..19155
                     /gene="rpsO"
                     /locus_tag="PC479_001460"
     CDS             18898..19155
                     /gene="rpsO"
                     /locus_tag="PC479_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2804009.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            19395..20240
                     /locus_tag="PC479_001461"
     CDS             19395..20240
                     /locus_tag="PC479_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2804010.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            20365..22476
                     /gene="pnp"
                     /locus_tag="PC479_001462"
     CDS             20365..22476
                     /gene="pnp"
                     /locus_tag="PC479_001462"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2804011.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            22742..23485
                     /locus_tag="PC479_001463"
     CDS             22742..23485
                     /locus_tag="PC479_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2804012.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            23643..24890
                     /locus_tag="PC479_001464"
     CDS             23643..24890
                     /locus_tag="PC479_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2804013.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            24925..25170
                     /locus_tag="PC479_001465"
     CDS             24925..25170
                     /locus_tag="PC479_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2804014.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            25180..26403
                     /gene="dapG"
                     /locus_tag="PC479_001466"
     CDS             25180..26403
                     /gene="dapG"
                     /locus_tag="PC479_001466"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2804015.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            26516..27250
                     /locus_tag="PC479_001467"
     CDS             26516..27250
                     /locus_tag="PC479_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2804016.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            27373..29784
                     /locus_tag="PC479_001468"
     CDS             27373..29784
                     /locus_tag="PC479_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2804017.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            29787..30845
                     /gene="mnmH"
                     /locus_tag="PC479_001469"
     CDS             29787..30845
                     /gene="mnmH"
                     /locus_tag="PC479_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2804018.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            30839..32173
                     /gene="rimO"
                     /locus_tag="PC479_001470"
     CDS             30839..32173
                     /gene="rimO"
                     /locus_tag="PC479_001470"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2804019.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            32160..32702
                     /gene="pgsA"
                     /locus_tag="PC479_001471"
     CDS             32160..32702
                     /gene="pgsA"
                     /locus_tag="PC479_001471"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2804020.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            32990..34036
                     /gene="recA"
                     /locus_tag="PC479_001472"
     CDS             32990..34036
                     /gene="recA"
                     /locus_tag="PC479_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2804021.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            34212..35753
                     /gene="rny"
                     /locus_tag="PC479_001473"
     CDS             34212..35753
                     /gene="rny"
                     /locus_tag="PC479_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2804022.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            36067..38403
                     /locus_tag="PC479_001474"
     CDS             36067..38403
                     /locus_tag="PC479_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2804023.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
BASE COUNT        15511 a         4060 c         7222 g        11627 t
ORIGIN      
        1 atttgagata taagagggag ttataaatca ttagttttga ggtgaatgtt atgagcgata
       61 aagaattatc tattttgttg ttagaaagta tgaataattt tcataattta ataaagatta
      121 ttaataacga aagatataaa aaagataagg ttcttacaga aagacagttc tttgccttag
      181 taaaaataag aaaacatgat aaaatagagc ttaaaaatct aagtagagat ttgcatgtat
      241 ctacttctag tctgtgtata ctacttaata aattggttga gcaagagtat gtatataggg
      301 aagaagatag tagagataga cgaaatactt tttatggaat aactaaaaat ggagaaaaga
      361 tattggataa tgaaatacta aagtttgtat ctataataag tgataaaatg gattgcctag
      421 atataaataa taaagataaa ttatttactt ctttggaaga gtctaaaaat ataatagaac
      481 aattattttg aaaaggagaa tttattatga atataaagta ttctaaagaa atattaaaaa
      541 gtttagaaaa tgctataaca gtagtagctc tatttgaggg agaagatgtt tctacgacat
      601 ttgataaaat aaatatgata agcagaaatt ttaattctaa agaaaatagt atagatactg
      661 taacattggt tgaaaataac aatttggtaa atgtttgtct tgttggtttt ggtaagagag
      721 agttattgaa tagagaaaaa attagaatca taggtgggaa tttgtgtaaa aaattaaaaa
      781 atatattgaa aaatgaagca tataagagta gtgatgttaa tgtactgaat cttaatttaa
      841 atagtgatga aataggttgt tttgtagaag ggatattact tggaaattat aagtttgata
      901 aatataagac aaaaagtaag gattttgaaa gaaaagaagt tgatactata actatattta
      961 ctgaaaaaga agttgagtgt caaatagaaa aggctaaaat attagctaat tcaactataa
     1021 tagctagaaa tctagttaat gaaccatcaa acgtaatata tccaaaaaca ttagcagtag
     1081 aaacagttaa acttggtgca gagtttgggt ttaatgttga tgtttatgaa gaagaaaaaa
     1141 taaaggcatt gggaatggat gcttttttcg ctgtttcaag aggttcaatt aataaaccaa
     1201 gattcatagt aatgagatac tttggagata aagaaagtac agatatatta ggtctagtag
     1261 gtaaaggatt gacttatgat actggtggat attctttaaa atcaaatgct tctatgctag
     1321 atatgaagac agatatggct ggtgctgcga gtgttatagg tgctatgtgt gctatatctc
     1381 aaagtaaatt aaagaaaaat gttatagcag tagtggcagc atgtgagaat gctttatcag
     1441 gaggttcata caaacctgga gatataatta gttctatggc aaagaaaact attgaagttt
     1501 taaatacgga tgcagaaggt agacttacat tagctgatgc aatatactac attataaaca
     1561 atgaaaaagt aacaaaggtg gttgatgtag ctacattaac tggtgctgct ttaatattgt
     1621 taggtaatgt cgcaactcct atagtaacaa ataatgatga tttttattgt gaattagaaa
     1681 aagctgccac tttatcaggg gaaagggtat ggaagatgcc aatttatgat gaatttaaag
     1741 atatgataaa aggagaagaa gcagacctta aaaacactgg tggtaaaaat gctggatgta
     1801 taacagcagg agcttttatt ggtgaatttg ttgggaatac tccttggata catatggata
     1861 tagcggggac aagtacatca tcaaaatcaa ttggatacaa agcaaagggg gctacaggag
     1921 aacctgttag aacattgtac tatttggcag aaatctaaaa aacaattata aaatataatt
     1981 tataaagcca cgtggatatt tttgtaattt aagattatct atgtggcttt attttttatt
     2041 taaataaatt atttactctt tttctttttt tcaagtataa aagtatctct attaaataaa
     2101 agagcaggta tgtacataat actagtaaca aaaataaacg taaaaatagc agttgtgcta
     2161 tgacttaaaa aaccataata gtacctagag ttggtaaatg tgtttagtaa aaatatgaat
     2221 gaaaaataaa taaaacatat agctaagtta tctctaagtg catatctgtt aactctaaat
     2281 tttctaacat caaccatata tatatctgca taaattatca agcacttaga gaagataaag
     2341 ataaaatata ttaagctagg aaattcatag cccaagtttt tagaaaaaat gagttctcca
     2401 actgaacaaa ctaaactcca agaaaaaaat atattaaaaa gatgatactg cttaaaaatt
     2461 agtatggcta tacacagata tatacaaata taattgattc caattggtaa agagtttaga
     2521 gtactataat tacctgaaga aataagtaat aattgctcta gagccataat tattacaagt
     2581 aatgtacaaa ttatcttctc aactgtatag ctataaggta aaatattttt ggttagtttt
     2641 ggacaatagt ataaaaataa tccaaatata aagagactta caaattgttc actagaaaaa
     2701 agaaaaattt taaacatata acattctctc ctataataaa tttaatatgt atctatataa
     2761 atatattcat tatatctcaa tttaatatat aaaaaagata ataatagtta gattttttat
     2821 atgaatttta ataggtaaaa tatcctattg ttttgtagat atatcctaat tatgtcaaaa
     2881 aataaaaaaa tttttattta ttagagtttt tggaaataat aataacagat attataatta
     2941 attaacagtt tttgtaagta tttgacaata gagagggtgt aacaaatggg aataatagag
     3001 tcagcatcaa aattggctga gatggttcat ttattggctg ttgaaaaggg gataactgat
     3061 atagaagcat gggatgaagc tgtgaaagaa tacagcaaaa tttatgaaga aagaagaaat
     3121 gaatagaatg ataaaaaatg aaaacttgat gataaaaaat aactttattt aaagaaattc
     3181 aatttttata gaagtattaa aaataatata aataaaaaga tggtatttca aatgtaagta
     3241 aaaaatcatt ttgggatacc atccttatta tttattttaa taatatataa gtaataaatc
     3301 ttacttatcc atatttttta tgtaacctct tttttctaac ttttttatat gctccttacg
     3361 tatttcatca agattaatac catattgttc ttctagctta aacatcatag taacacatgt
     3421 ctgagctaca tccattagtt ccttagcagt tttctttata atatcaactt catctaaatc
     3481 attattttct ccacttagat ttctgaattt acctatacat tcagctaatt caccttgttc
     3541 ttcacccatt ttgataaatg tagattctag tgttgggtct aaattattaa gctctggtaa
     3601 agatatagtt tttagtttca taaaagctcc tttcaagtta ttcgtattta tactaaaaca
     3661 tcataacaaa aataatacta aaaaaaaact ttaaattcaa cataaaaact tacatatata
     3721 ataaaaatcg tgaaataaat ctatatttgt cttaatacag cctcatataa aacttctcca
     3781 ataggtttat ttattacaag agaagctgac ttatcataga ctgtatttgc tttattaata
     3841 agagcaatat aatcaccttt gtaataatct ataaaacttg ctgctgggta cacagctaat
     3901 gatgtacctc ctataatcaa taaatcagca ttacttatag cagaaattgt tttagtaatg
     3961 acatcactgt caagagcttc ctcataaagt actacatctg gttttactat agaaccacag
     4021 ttgtcacagt aaggaatatt acctcccaga tttaacatag attctaagtc aaaaaactta
     4081 ccacatttag tacaataatt tctatgtaca gaaccatgaa gttctagcac atttttactt
     4141 ccagccattt gatgaagtcc atcaatattt tgagttatga cagctttaag cttacccatt
     4201 tcttcgagtt ttgctaaagc tatatgagca ttattgggtt ttgcattagg atatattaat
     4261 ttatccttat aaaattcaaa aaattcttct ggatatctaa caaaaaaagt atgtgaaact
     4321 aattgttcag cagtaaattg tttattgagt ttttggctaa ataatccagt agaacttcta
     4381 aagtctggaa tattagattc agttgaaaca cctgctccac caaaaaatac aatattattg
     4441 tgattagcta ttaagtcttt taagctattt gcatccatag ttacacctcc aaattattat
     4501 atataaattg tactacatat taggtcgttt atgttttatt aatttaaata aatatatagt
     4561 ttaataagtt aaataaaggg aatataaata aaatgtatac agagtataaa tagacaatat
     4621 ttactaaaat aacaagttgt cgtataatta tattaaaatt gttttagtgt ataagaaggg
     4681 cgggggaagt acgtgaagaa aaaagcagct ttagcaacat tggctatgtt acccttaggt
     4741 gtggtaaatg cacatgctga tggagatata ggtatagtga ctataaatta tttaaatgta
     4801 agaaatgaac caactgccga aagtagcata gcctttgttg ctaaaaaaga tgataaagtt
     4861 ttgattaaag actcttctaa tggatggtat aaaataaaag ctgaatctgg acaagaaggt
     4921 tgggcttcat caaaatatat agcaaaatca aatagtgact ctttaagaac gtctacaaat
     4981 aaagaaaaac aagttatttc aaatagttta aatatgagaa atggagcagg tacaagttac
     5041 agagtaataa ctgtactaaa aaaaggtcaa aaagtagaag taatatcaga gagtaatgga
     5101 tggtctaaaa tcaaatatga tggaagacta ggatatgtat ctagctctta tttaggagac
     5161 gtttcaaact caactaataa atcaaaaact aaacaagtga atacaacttc actaaatgta
     5221 aggagtggac caaatacaag ttatggtttg ttaggcaagt tgccaaaagg aagtaaagta
     5281 gaagtaatat cagaaggtaa tggatggtcc aaaataaagt ataatggaaa agatgcctat
     5341 gtatctagta tgtacttatc ggatgtaagt caaagtaatt cagacaattc tagtcaaagc
     5401 aatgataaaa agaatactga taaggtcgta aatacagctt ctttaaatgt aagaagtgga
     5461 ccaggttcta catatagtaa gttgggaaaa gtttataaag gaagtaaagt aactgtacta
     5521 tcagaaagta gtggatgggc taagattaat tttaacaata aagaagcatt tgtagtaggc
     5581 aattatttat ctacttcagc agatacttca aataataact caaatagtaa ctctgataac
     5641 agttctaata gtaatggcaa taattcttca tcatcaggtc aagtaaatgg tatgtcaggt
     5701 ataagtgggg ctaaaattga ttataaatct ttgagttata ctttagaatc tcatataagt
     5761 aaacaagttg agaaagcagc atcaggagga aatgtgatag ctccaagtaa taggaaaagc
     5821 actccaagtc ctgaatttag tactttttca gctcaaagaa caagctcatt tgtaaatgca
     5881 agttcaagtg atatagaata ttatttaaat cctaaaaatt ttacaaatac tactaaaggt
     5941 atgatgcagt ttttaaagat taacagttat agagatggta tttcagaatc aagtcttaac
     6001 tcgtatctta atggtttatc ttctagtgta tttaaaaacc agggagcagc ctttataaat
     6061 gctgccaaga agtataatat agatgttgta tatctagtat ctcatgctat gtgggaaact
     6121 gcttatggta agtctacact tgcccaaggt caaacattaa catcttataa gggacaacct
     6181 cttagtaagc cagttaaagt atacaatttt tttggtatag gagcaataga taaaagtgct
     6241 aatgtttcag gtgcagaagc agcgtattcc aatggctgga ctagtgtaga agctacaata
     6301 gatggctctg caaagtggat atcacaaaat tacgtaaata gttctaagta caatcagaat
     6361 actatctaca agatgaagtg gaattatgat tatacatggc atcagtatgc aactgatgtg
     6421 aactgggcta atggaatttc tggaattatg gaaaatttaa ttggtcttta tggaggagga
     6481 agtagtttgg tttttgaagt tcctcaatac aagtaataca taaataaaaa atatagacta
     6541 tgggtagttt tcattttatt taaattatct atagtctttt tgtatataat ttataatctg
     6601 taaatttaat gctaacaaaa cttttatgtt ataatattaa ttctaagtaa atataagtga
     6661 gtaataggta gaaatagata aaaagtggta ataatatata taaagataat tttgagaaag
     6721 gaggatatat atggaaagta ttaaggaata tttggataag ttagaaatta ataatagtgg
     6781 tcttggaaag caacttaaag aagtttatat aaatagagta gtttatttta aggaagataa
     6841 gatagtttat ttttatctaa cttctaaaga tattgtttct catgaacttt tagataaatt
     6901 taaagaagaa ttgatgtata aactcgatta ttttaaagat atgaggatga agataagatt
     6961 tactggtctt gaaagaaaat ccaataaaga tgttataaaa aaatattgga ataatatatt
     7021 gtatatatta aagtatcttt gtccatctat tgcagggtgg tataaacaag tagaatttct
     7081 ttgtttagaa gaagaattaa aaataaaact tccaaaaggt atattttatg agcgattaat
     7141 gaaaaaaaat gtaacttatg tacttaaaac cgtgcttagc gaagagttgg gattagattt
     7201 aaatataaca atagaaaaag ctgttgatga aaaggtaaat aaagaaagac tcataagaat
     7261 caatgataga gaaatggaag aaaaaattag ggctttagaa attggaaaag ttaataactg
     7321 tgaagaaaat gaagaggaaa gttatgtcat taagtctgat gtagatgaaa atttaatcta
     7381 tggagataat gcaaatgcta tgattgaaaa tatagttgaa ttaaatgcat cttctggaac
     7441 agttgcagtt gttggagata tatttgatgt tgacactaaa gaacttaaaa acggaaaaat
     7501 acttatgata gcttcaatta cagattatac tagctcaata agctgtaaac tttttttaac
     7561 agatacaaat aaggatggag tacttgaaaa tgttaaaaaa ggagcatact taaaaatcaa
     7621 gggtgacata gtttatgata catatcaaag agaaattagt atgatgatta gtgggataag
     7681 aaaagaaact agaatagaga gagaagataa atcagaggaa aaaagagtag aactccatgc
     7741 acatacacaa atgtcttcta tggatgctat ttgttctgtt aaaaagcttg tagagcgtgc
     7801 agccaaatgg ggtcattctg ctattgctat aacagaccat ggtgttgtac aaggatttcc
     7861 agatgctatg aatgcaggta agtcaaataa tataaaaata ctttatggag tagaagggta
     7921 cttggttgaa gatgattctc tcattataga ggatgcaaat gataaagaac tttcgcagac
     7981 atttgttgta tttgacatag agacaactgg tttttctaat acaaatgata aaataactga
     8041 gattggtgct gtaaaaatag aaaactttaa gattgtagat agatttagtg agttaataaa
     8101 tcctgaaaaa gatatttctt ataagataca ggaattgaca ggaataacta atgaattaat
     8161 taaagacaag ccaaccattg aagaagttct tcctaagttt atggaatttg ttggagatag
     8221 tgtattagta gcacataatg cagagtttga tactggattt atatcgcaaa aatgtaggga
     8281 acaaggcttg ttatataata acaaaaaagt agatacactt atgttagcaa gagtaatgtt
     8341 gccaaactta aaaagacata gattaaatgt cgttgcaaaa gaacttggta ttcctctttt
     8401 aaatcaccat agagcagtgg atgatgctga agctacagca cttatattta ataagttttt
     8461 gcagatgctt acagaaaaag gtgcaaaaac tctaagtgat gtaaacaata tacttggaaa
     8521 gattgattac acaaagctta gcacaaatca tataaccttg atagcaaaaa attctatagg
     8581 tataaaaaat ttatataaaa taatttcaga tgcacatgta aatcattttt ttagagcacc
     8641 aagaatcctt agaagtgtat taaatgaata taaagaagga ttaataattg gttcggcttg
     8701 tgaagctggg gtggtttttc aagctgtaaa gaaaaacgtg tctgatgagg aaatgaaaaa
     8761 aataatagat ttatacgatt atattgaagt aatgcctata gataataata gatttatgat
     8821 tgataaagga gaagtaaagg acgaagaaga gttaagggag ttgaatagga aattaattga
     8881 tacagccaaa aaatttgaca aaattccagt agctactggg gatgttcatt ttttagacaa
     8941 acatgaagct gtcttaagaa aagttcttaa atattctcaa ggttttaaag tagatgaaga
     9001 agaaacttat ttacatttta gaacaacaga tgaaatgttg gaagagttta gttatcttgg
     9061 agaagaattg gcatatgaag ttgtagttga aaatagtaat ctgattgccg atatggttga
     9121 agatataaaa cctataccag atgatactta tcctccaatt atagagggct cagatgtgga
     9181 gttaagagaa atgtgttaca aaaaagccaa acgaatctat ggagacccaa taccagaaat
     9241 agttcaaaaa agactggata gagaattaaa ttcaataata agcaatgggt atgcagttat
     9301 gtatattata gctcaaaaac tggttgcaaa atctctttca gatggatatt tagttggttc
     9361 aagaggctca gttggctcat cttttgcagc tactatgagt gatataacag aagttaatcc
     9421 tcttccagca cattatattt gtgaaaacga agactgtaaa tattcattct tttatgaaat
     9481 aggtgaatgg ggctcaggtg ttgatttacc agacaaggat tgtccaaagt gtggaagaaa
     9541 acttgctaaa aacggtcatg atattccatt tgaggttttc ttaggttttg aaggtgataa
     9601 agaaccagat atagacttaa atttctcagg aaattatcag ccaacaattc ataaatacac
     9661 agaagaacta tttggagagg ggtatgtata tagggctggg actataggta ctgtagcaga
     9721 aaaaacagct tttggatacg caagaaaata tgtggaagaa aataatataa gtgtaccaaa
     9781 tgcagaagta cttaggcttt caaatggatg tactggagta aaaagaacct caggacaaca
     9841 tcctggtgga gttatggtta taccagacta taaagatgta tacgatttta cacctataca
     9901 gtatccagca aatgatactt cttgtggtgt tataactact cactttgatt atcattcaat
     9961 aagtggtaga atacttaaac ttgatatact tggacacgac ggtcctacca ttataaggat
    10021 gcttgaagat atgactggaa taaatataac agaaattcct ttagatgata aagagactat
    10081 gtctctattt acatctacag aagcattagg tgttacacca gaagaaataa actgtcctat
    10141 aggttgcctt gctgtaccag agtttggaac taagtttgta cgacaaatgt tacttgacac
    10201 aaaacctact acatttgcag aacttgtacg tatatctggt ctctctcatg ggacagatgt
    10261 ttgggtaaat aatgcacaag acttagttag agaagatata gttggattaa aagatgttat
    10321 atctacacga gatgacataa tgaactattt aatatttaaa ggattacctc ccaaaatgag
    10381 ctttacaata atggagagtg taagaaaagg taaaggttta aaaccagaac acattgaaga
    10441 aatgaaaaaa aataatgttc cagaatggta tataggctca tgtaaaaaga ttaagtacat
    10501 gttcccaaaa gcacatgctg ttgcttatgt aatgacatca tttagaattg catattgtaa
    10561 agttcactat ccagaagcat tttatgcgac ttattttacg acaaaagttg aggattttga
    10621 tgcagattta attgttaaag gattagatgc tataaaatca aaaataaatg aaattgagtc
    10681 actgggaaat gatgcaacca caaaagaaaa aggtatgttg accgttttag aagttgctct
    10741 tgaaatgtat gctagaggaa taaaattatt accagttgat atatataaat cagatgcaac
    10801 tgagtttata gttgtaggag aaaaaacatt acttccacca atggcagcaa tacaaggtct
    10861 tggaggaaat gcggctataa acatccaaaa tgaaagaaaa aatggtgaat ttatatcaaa
    10921 agaagatttg agaaagagaa caaaaatatc aaaaacggta atagaaactc ttactaatca
    10981 tggttcactt gagaatatga gtgagagaaa tcaattgtca ttgttttaag ttagcccaaa
    11041 aagttgcaaa taaaagaatt ttatgatatc atagtataga aaatagtata ctttgcagaa
    11101 actaagagtg agacagctgt tctcactctt ttattttaaa tatattatta aaaagctggt
    11161 ttatttttat taaatatatt ttatttaaga ttatataaac tggtcattta caattgaata
    11221 ggaggaactt taatgaaaaa aaatttagaa gctacaattg aggagattgt aactaaaata
    11281 acggatgaac atggatttga aatggttgat gttgaatatg taaaagaagc aggagaatac
    11341 tacttaagag tatacataga taaagaagaa ggaatatctt taaatgaatg tgaattagtt
    11401 agtagagaat taagtccaat actagatgaa aaagacccta taaaagaaaa ttacttttta
    11461 gaggtatctt ctccagggct tgatagagca ttaaaaaaag acagagattt tgttagatat
    11521 caaggtagag atgtagattt aaaattatat aaaccattaa atggatgtaa gcaatttgaa
    11581 ggtgaacttg taggattaac agaagataat aatataaaaa tcattgcaaa tggtaaggaa
    11641 atggaattta atagaaaaga tgttgctatt gtcagattag caataaagtt ttagtggtat
    11701 attaaatagg gaggatataa aaaatgaatc atgaatttat ggaagcatta gatgaattag
    11761 tagttgacag aggtatagat aaagaaatac ttatagatac tatagaacaa gctcttttaa
    11821 ctgcatacaa gaaaaacttt ggttcagcac aaaatgttag agttgaattt gatagagaca
    11881 agggagatat aaaagtattt tctcaaagag ttgttgttga tgaatcagat ttatatgata
    11941 ctttcttaga aatagagtta gcagatgcta gagagataaa ccctaattat gaattaggtg
    12001 atattataga acatgaagtt acacctaaaa actttggaag aattgcagct caaacagcaa
    12061 agcaagtagt tgttcaaaga ataagggaag ctgagagaga aatagtgtat aatgaattta
    12121 tagaaaaaga aaatgaaata gttacaggag aggttgctag agcaaataaa aatgttgttt
    12181 atgtaaatct tggaagaata gaaggtataa tgactcaaag tgaacaaata ccatttgaga
    12241 attataaagc aggtcaaaaa ataaaattct atatacttga agttaaaaag actaacaaag
    12301 gacctcaaat agttgtatca agaagtcatc cgggtctagt aaagagattg tttgaagaag
    12361 aagttcctga aatatatgaa ggaatagttc aaataaaatc tgtagcaaga gaagcagggt
    12421 caagaactaa aatggcagta agatctatag atgaaaaaat agacccaatt ggagcttgtg
    12481 ttggaccaaa aggacttaga gtaaaaaata tagtggatga acttggcgat gagaaaatag
    12541 atataataaa atatagtgaa gacccagcag agtttatatc agcagcactt agcccatcaa
    12601 aggttgttag agtagatata gatgaagatg aaaagtctgc cttagtaatc gttccagatt
    12661 atcagttatc gcttgctata ggtaaagaag gtcaaaatgc aagactagca gcaaaattga
    12721 ctaactggaa aatagatatt aagagcgaat ctcaagcaga acaagaaatg ttaaatcttg
    12781 aaaattccaa agaaatagaa gctgaggatg ttgaagctga atttatcgac caagaagagt
    12841 tagatacttc agaggataat tttagtgaag aagagtaagg agggatgatt ttgaaaaaag
    12901 taaaaaaagt gcctcaaaga aaatgcatag catgtcaaga tagagactct aaaaaagagc
    12961 ttataagaat agttaaaaat aaagaaggaa aaatattttt agacccaaca ggaagagcca
    13021 atggaagagg agcatatgta tgtaaaagct ctgaatgtct aaaaaaagct ataaagagta
    13081 aagcattaaa taaagctttt aaaatagatg ttcctgatga agtctatgat aatctactta
    13141 tggagttaga agaatatgaa aaataataaa gaaaaagtac tttcgttctt agggctcgca
    13201 actcgtgcag ggaaaatagt gtctggtgat gattcaactt tgcttgacct taaaaaagga
    13261 aaagtaaatt tgattttaat cgcagaagat gcatcaaata atactaaaaa actttttaaa
    13321 gataaatcaa cttttagaaa tattccatat ctattttttt caacaaaaga ggaaatagga
    13381 tttgcaatag gaaaatctcc aagagcagtt gtaggaataa aagatgaaaa tttttctaaa
    13441 aagataattg aactaataga aatttaaaat aaaatagggg gtgatcttgt gtcaaaaaca
    13501 agagtatacc aaatagcaga agaattaaat atatcaaatg aagagcttat aaataagtta
    13561 gctgaattag atataaatgt aactgacaaa gattcagtat tagaaggtga agagttagaa
    13621 ttagcacttg aaatgttagg tgaagattta tctcaagaaa atggaaatgt tatagaaata
    13681 gatggaaagt tgacagtaca agtattagca acgaaattag ataaatctcc atcagaaata
    13741 attatgaagc ttatgaaaat gggaactatg gcaacaataa atcaagagat aagttttgag
    13801 attgctgctt tggctgccaa agactatgga tttgaattaa cagtagctga aagtgatgat
    13861 acagaagctt tagaaataga agctttaatg gaaattgaag aagataaaga agaggattta
    13921 aaacctagac caccagttgt tacagttatg ggacatgttg accatggtaa gacatcttta
    13981 ctagatgcaa taagaaaaac tgatgtgatt tcaggtgaag ctggaggaat aactcagcat
    14041 ataggtgctt cagaagttaa aatcaatgga cacaaaatag tatttttaga tactccagga
    14101 catgaggctt tcacatcaat gagagcaaga ggagcacaag ttacagatat agcaatatta
    14161 gtagttgcag cagatgatgg tataatgcct caaacagttg aagctataaa ccatgctaaa
    14221 gcagcaggtg tacctttaat agttgctatt aataaaatag ataaaccagg tgcaaaccca
    14281 gataaggtta aacaggaatt agcagaccaa ggattattag ttgaagactg gggtggagaa
    14341 gtaatagcag ttccagtatc agctaagaaa aaagaaggaa tagatacact tttagaaatg
    14401 gtattattgg ttgctgaaat ggaagagtta agagctaatc caaataaaag agcagtagga
    14461 actgttatag aagctgagct tgataaagga agagggccag ttgcaacagt acttgttcaa
    14521 ggtggtactt taacagtagg tgacccaatc gttgcaggtg ttgcatgtgg taaagttaga
    14581 gcaatgataa atgcaaaagg taagagagta aaaacagcag gaccatctac agcagtcgag
    14641 atattaggtc tatcagaagt tccacaagga ggagaccaat ttgtcgaagt tccaactgat
    14701 aagattgcta gaagtgtagc agcaagaaga caacaaatag ttagagatga aatgttaaaa
    14761 tcaactcaaa gattatcact agatgctcta tttagtcaaa tgagtgaagg ttctataaaa
    14821 gaccttaata tagttataaa agcagatgta caaggttcag tacaggcagt taaacaatct
    14881 ttagaaaaat tatcaaatga agaagttcaa gttaaagtaa tacatggtgg tgttggagct
    14941 gtaacagaat cagacatatt acttgcagca gcttctaacg caataataat aggattcaat
    15001 gtaagaccag ttccaggtgc agaatctcta ggagaaaaag aaaatgttga tataagaaca
    15061 tatactataa tatacaaggc aatagaagat atacaagcgg ctatgactgg tatgttagac
    15121 cctgaatatg ttgatgaaga aacaggaaaa gctgaaataa gagaaatata taaaatatct
    15181 ggtgttggaa cagtagctgg atgttatgtg actaatggta agatatttag aaattgtaaa
    15241 gtaagattag ttagagatag cataataata catgaaggtg agcttgcagc acttaagaga
    15301 tttaaagatg atgtaaaaga agttaattct ggatacgaat gtggtatgag ttttgtaaat
    15361 tataacgaca taaaagaagg cgatatagta gaagcttata taactaaaga agtagaaaga
    15421 aaattataaa ttcgtatcaa agaaaatcag ttagtaaaga aggtcgatgg tatggcatca
    15481 tataatagaa caagaaggat agcagaagaa ataagaaaag tcgtaagcac tatgcttata
    15541 aatggagtaa aagaccctag aataacatct atggtgagtg taactgatgt agaagttaca
    15601 aatgacctaa gatatgctta tgtatatgta agcattctag gtggagatga agaatccaca
    15661 ctaacaggtc ttaaaagtgc tggaggatat ataagaagag aagtaggaaa aaatataaaa
    15721 cttagatata ttcctgaaat agtatttaaa cttgatgatt caatagaaaa aggaatgtat
    15781 atggatagcc ttataaaaag agttaatgaa aaaaatgctc aacaaaatga ggatgaaaat
    15841 tatgatgaat aatatggata gtattataaa ctatatttat gagggtgact attttatagt
    15901 tacctctcat atcagtccag atggagacaa tattggttct acactttcaa tgtattatac
    15961 attgaagaat ttaggaaaaa atgtatatta tgtacttgat gatgaagctc cattaaattt
    16021 aaggtttttg gttgaagatg taaaaatcta taagtctaat gaatttaata tgaagaacta
    16081 ttctctaatt gcacttgatt gtggggataa gatgagaata tgtgtgagtg atgaaatcaa
    16141 aaataatgca tcaaaattaa tctgtattga ccatcatgct agtaatgatt catatggaga
    16201 tttaaattat gtagatattg atgcatcttc tacatgtgaa ttagtttata atttattagt
    16261 tagatttcag caaataaaag atattaatat tataaatgaa gatatagcaa catgtttata
    16321 tacaggtctt gtaactgata ctggaaactt ttcgtattca aatgtacatg caagtagctt
    16381 tgaaatggca aaaaatttac ttgttttagg tgcacaaaaa aatactataa tacaaaatat
    16441 atatcaaagc aattcttctg gttattacaa attattagga gaagctctaa agggtttgga
    16501 gatatttgat tcaaaggtat ctagtatagt tttaactcaa gacatgatga atagaaataa
    16561 tattagcttt aatgatgttg atggaataac aacatataca agagacataa aaggtataga
    16621 agttggaatt ttatttaaag agaaaaaaca aaatgaaatt aaagtgagtt ttaggtcaaa
    16681 aaattatgtt gatgtaagtg aaatagccaa gttatttggt ggtggtggtc atgtaagggc
    16741 tgctggatgt actattagag atagtattga taatgcaaag aaaatggtac tagaggcagt
    16801 gttaaaatca atttaaggtg acagtaaaat gaataagatt ataagtatat tgaaacccac
    16861 aggtatgaca tctcatgatg ttgtaagtag agtgagaaag attttaaata taaaaaaagt
    16921 tggtcataca ggtactcttg acccagatgc ttcaggtgtt ttaccaatct gtatagggaa
    16981 agctacaaaa gtatgtgaag ttattttaaa caaagataaa tcgtatatat gtgagcttac
    17041 tttaggtata agtacagata cttatgatgc ttcaggtgaa attttaaaaa aagttgatga
    17101 ttttaaattt agtaatgaag acatagaaag agcttttgac acacaaagag gagagataaa
    17161 tcaacttcct ccaatttatt cagctttaaa ggtaaatgga aagagaatgt gtgatttagt
    17221 aagaagtggt agacaatctg aaattacttt aaaaacaaga agagtaaata taaaagacat
    17281 aaaaatacta agtataaaag gtaataaagt aatgttttat gttgagtgtt ctaaagggac
    17341 ttatgtgaga agtatttgcc atgatatagg agaatatcta ggttgtggag ctcatatgtc
    17401 gtttttgaat agaacatctt ctggtaaatt tgacttagat aattcaataa cattagaaga
    17461 attagaatta ttttatgaaa ataaaacttt agacaaatac ttatatgata tagattatgt
    17521 ccttgattcg tttaattatg ttgtattaaa ccctaatgct ataaaatatt atagtaatgg
    17581 tggctcaata gatgataaaa ggtttttaaa gaataatttc gataaagatg atgaatttgt
    17641 aagagtatac agtacagata atttcttagg attaggaaag ttatcaaaac acaataatac
    17701 aataagtgtt aaaagtgata agatgttcat ataaccaatg tatttaaaaa aacaacaaaa
    17761 gagatagtac tataataact tctatagtct atctctttta cttatgctat aatagtttgt
    17821 gaataagtta agagtaaatt atattttagc ggggaacatt tattatggtg attataaaat
    17881 ctataaaaga aatagcaaac ataaaagaaa gtgtcattac cataggaaat tttgatggtg
    17941 tacacaaagg acatcaagtt cttataggaa aaacagttga acatgcaaaa aaagaaaata
    18001 tacagagtat tgtatttaca tttgcaaatc atcctgtaaa ttactttaga ccaaactcaa
    18061 caaaaaatat tataagcaat gatgataaaa taaaattatt agaagatttg ggaatagata
    18121 tagttgtaat cataccattt gatgagtata tgactaaaat acctgcaaag gatttcgtag
    18181 atgagatact agtaaaaaaa ttaaaagcta aaaagatagt aattggacat gactttacat
    18241 ttgcaagggc aaaagaaggc aatgttaatc tattaaaaag cttagaacat gaatttggtt
    18301 ttgaggtaga agtaataaaa cccatcaaaa tcaatgatgt aagagtgagt agtacttata
    18361 ttagaagttt agtatcccag ggtgatatgg ctaatgtcaa ggaatatttg ggtagaaatt
    18421 acaagttaga aggttgtgtt atacattcta aacaccttgg aagaactata ggattcccaa
    18481 cggcaaatat tgaccttaag aataatatgc tcgttccaaa gagaggtatt tatgcttcta
    18541 ttgtacatat tggagatgag acttattttg gtgctacaaa tgtaggctat aacccaacag
    18601 ttaatggaaa aactttgtct attgaaacaa atattttaga gtttgataga gatatttatg
    18661 gggaaaatat aattgttgaa tttttggaaa gaataagaga tgaaagaaag tttaattcaa
    18721 tagatgactt aaaaaatcaa ttgtataatg atacaaatta tgtgtatgaa aactatgttt
    18781 gcaaaaacaa gtaatgtatg ataaaatgat taaggaaaat aaccattact cggcatttga
    18841 atactccaat caatgcttag taataggcgt ttctaaaaaa atacggaggt aatctaaatg
    18901 ataaacaaat cagaaataat aaaagaatat ggaagagctg aaggggatac tggttctcca
    18961 gaagtacaaa tagctttatt aacagctaga ataaacgagt taaatggaca tttaaaaact
    19021 cataagaaag accaccattc aagaagagga ctattaaaaa tggttggtag aagaagaaac
    19081 ttattagctt acttaaagga aaaagatctt gaaggatata gagcattaat agctaaatta
    19141 ggattaagaa agtaatttca ttctttataa aaaagcaggt atagtgtata cctgcttttt
    19201 tatagttaat tcattataaa gtaaaatagt taataataaa agtagtttat atgttataaa
    19261 aaattataaa atgaataata ttgggtaaga atattaaaat agtatacaaa tacataatca
    19321 atttaaaagg aaagacagga taaatataga aattttataa caggataatt agttggaata
    19381 aggaggatat tattttgaaa ataaagttag tatgcgatag tttgtgcgat attccagatg
    19441 aaatatcgga aaaagattat atagagatag ttccacttac agttattttt aatgataggg
    19501 aatatataga aggagtagat ataaagaaag aagagtttta taaaaaagta aaagaaatca
    19561 agcaaatacc taaaacttca caagctacat atatggagtt taaagaagtg tttgataagt
    19621 tcataacaga aggttatcat ataatctgta tgacaggggc tgcaaacgca tcaggaactt
    19681 ttcaaagtgc tatgatagct aaaaatgatg taaatgaaaa tgaaaaaata catatatttg
    19741 atactagaaa tttatctcta ggtagtggac aatatgttat aaaggcatgt gaacttcttg
    19801 aagaaggact gggatttgaa gaaattatag atgaattaga aaatactcgc agtagtgtaa
    19861 gattgctttt tgcaccatat acacttgatt ttttaaagca gagtggaaga gttccagttg
    19921 caactgcttt gataggaaat atgttaaata taaagcctat atttttcttt gataatgggg
    19981 aagctaagtt ggtaaataag gttagaggaa taaagaatat agcaagtaaa ttggtcgata
    20041 taattttaga aatgaatgaa gggtctttag aaggaaaaat cgtcacaata ggttgtggag
    20101 ataatttaca cgattgtgaa atattaaaag atgaagtaaa taaaagaata aaagcaagaa
    20161 gagtattatt tactcgagga ggagtttcta tttgctctca tacaggacca gatatattgg
    20221 ctataagttg ttctaattag tagtaatata aggtttttgc atatatatgt gaagataatt
    20281 ttatattgtt aatttatagt atttttgata atatagtaaa tagagtgata tacgtgtttt
    20341 ttaataagag gaggacaagc attaatgttt gaacataaaa tatttaaaat ggattttgca
    20401 ggaagagaac tttctgtaga aataggaaag atttgtgaaa tggctagtgg tagttgtata
    20461 gttcgataca gtgatagtat ggtaatggta aatacaacta aatcagccaa acctagagac
    20521 ggaatagact tttttccttt aagtgtggat tatgaagaaa aattatactc tgtaggtaag
    20581 atacctggag gttttttaaa gagagaaggc aagccttcag agaaagctat acttacatca
    20641 agattgatag atagaccaat aagaccttta ttcccaaaag gatttagaaa tgatgttcag
    20701 gtagttgcaa cagttctatc agtagaccaa gattgtacac cagatatagt agcaatgata
    20761 ggttcttcaa tagccctttc tatatcagac ataccattta atggaccaac aggttcagta
    20821 tgtgttggcc ttgtagatgg tgcatttgtt gtaaatccaa acgcagagca aagagaaaaa
    20881 agttcaatgc atctagttgt ttcaggtaca aaggaagcaa taatgatggt tgaagccgga
    20941 gctgatgaag ttccagatga ggtaatgctt gatgctattt tatttgctca ccaagaaata
    21001 aaaaaaatag ttgagtttat agaaggtata gttgcagaag ttggaaaaga aaaaatgcct
    21061 gttgaattat accatgcagg agaagaaata actcaattgg ttcgcgaatt tgcaactgat
    21121 aagatgaaaa aagcagttca gacttttgaa aagttagaga gaatggaaaa tatggataga
    21181 gttaaagaag aaactttagc tcactttgaa gaaactttag aagattttga agattttgtt
    21241 ggagatatag aagaagtatt acaagatata ataaaagaag aagtaagaaa acttatagta
    21301 catgaaaatg taagaccaga taatagaaaa cttgaagaaa taagacctat atggtgtgaa
    21361 actggcatga tacctagagc tcatggttca gccatattta ctagaggaca aactcaagta
    21421 cttaatgttg ctactttagg tgctttagga gatgttcaaa aactagatgg gttagatgaa
    21481 gaagaaaaca agagatatat gcatcattat aatttccctg catatagtgt tggagaagct
    21541 agaccatcaa gaggtccagg aagaagagaa ataggacatg gtgctttagc agaaagagct
    21601 ttattaccag taataccatc tcaagaagaa tttccttatg ctattagatt agtttctgaa
    21661 gtattaagtt caaatggttc tacatctcaa gcaagtgtat gtggttcaac attatcttta
    21721 cttgatgcag gtgtacctat aaaagatatg gttgcaggta tagcaatggg acttataaag
    21781 catgatggta aggttgcagt gctttcagat atacaaggta tggaagacca tttaggcgat
    21841 atggacttta aagtagctgg aacagagtat ggtataacag caatacaaat ggatataaaa
    21901 attgatggta tagataaaga aattctacaa cgtgctttaa aacaagctaa agagggtaga
    21961 atccatatac taggagagat gagaaaaact atatctcagc caaaaccaga actttcacca
    22021 tatgcaccta agattgttaa aatgcaaata aatcctgaca agataaaaga tgtaatagga
    22081 cctggtggaa agataataac taagataata gacgaaactg gagttaagat agatattgaa
    22141 caaactgggg aagtatttat atcaggtatt gagattgata tgattaagaa agctcaagaa
    22201 cttataaata atatagtagt tgaaccagaa gtaggaaaaa cttataaagg taaggtaagt
    22261 agaataatga actttggagc atttgttgag atacttccag gaaaagaagg attacttcat
    22321 atatctcata tagctcatga aagagttgcc aaagttgaag atgtattgaa tataggggat
    22381 gaagttgaag ttaaagttac agagattgat gaaaaaggaa gagttaattt atcaagaaaa
    22441 gttcttttac caaaaccaga acataaaaat aaataactta ttatataatt aaaagcaaag
    22501 tcttaggatt ttgctttttt aaatacaaaa aagtagataa ttttaaaatg atagttatgg
    22561 atggcttata gttctttttc tgtcagtctt tactcttata aagaagctat ttaaaagctg
    22621 attaatagaa agctttattg atatatatag aatcacttaa gttatattta gtgcataaac
    22681 agagcttatt tttaatagta ttaaataaga aacaatttgt acataattag ggggagtttt
    22741 aatgataatg atggtactta ataagagtaa gctcaaaaaa atcataatac taattttagt
    22801 agtgttatta gtattatcag gaggggtaat ggtattaaag ttatttaaca acaagccagt
    22861 atttaattta tttaatggtg tagatttacc atatgaaaga ggtacaaagg ataaaagtgg
    22921 atatgttgca ttaacctgta atatagacct tggatgggaa acagagtacg tagaatccat
    22981 tttagaaaca cttaaaaaag aaaatgtaaa gattacattt aatgtaactg gaaaatgggc
    23041 agaaaagaat aaggatgaat tattaaagat aaaaaaacaa ggacatgaaa taggtaatca
    23101 tggatataaa catttagatt attctacatt atcatatgaa gacaattatg agcaaataga
    23161 gacctctaaa aagataattg aagaaattat aggtgaaaaa actaaatttt ttcaagcacc
    23221 agcaggttcc tttggtccag aaacagtaaa ggctgctaaa gcattagggt atacttctat
    23281 caaatgggat gccgatacta tagattggaa atacaaagac caaccagagg taatcattga
    23341 tagaatgaag aaaaaagaca ttaaagatag tagtataatc ttaatgcatc caaccaacgc
    23401 aacgacaaaa tgtatagatg atattatagc tattgtaaga gaaaaaggac taaaagcagg
    23461 aaaacttagt gatgtattta agtagacaaa gtataaatat ttggttaaga ttgtataagt
    23521 tgtagttgaa ttaattaaaa gactgactta taatgtaata catataaaaa ataaatataa
    23581 ttaaaacata gtgttttcaa taatccaata tgaaacaaag aataaattgt taggagaatg
    23641 taatgtataa gacaaaaata ctggaaaatg ggcttacaat aattggtgaa gaaataccat
    23701 atctaaagtc aataacatta ggtatttgga taaatgcagg ctctaggata gaagaagcac
    23761 aggtgagtgg aacttctcat tttatagagc atatgatgtt taaaggaacc aaaaacagaa
    23821 catcaaaaga gattgctagt tcaatagata atttgggtgg tcaaataaat gcatttacaa
    23881 gtaaagaatg tacatgttat tatgtaaaac taatagatga gcatatagat acaggaattg
    23941 atgtattgag tgatatgata ctaaattcta aatttgataa aaatgatata gataaggaaa
    24001 gacttataat attggaagaa cttaagatgt atgaagactc tccagatgac ttatcttatg
    24061 atttattagt ggaaaatata tatgctaatg atggattagg aatgaatata attgggacaa
    24121 aagaatctct atacaatata actagagaat ccatgttaga atacttaaat aagtattata
    24181 ttccaaataa cgctgttatt tctatagctg gaaactttaa ctttgatgat atggtggaga
    24241 aaatcaaatc aaaatttgga cactgggaaa agaaaaattt gagtatagac ataagtgagg
    24301 ctaagtttaa tccttgcttt atatcaaaaa ataaagatac tgaacaggtg aacttagcta
    24361 tgtgcctaaa aggtattcct tttgaaaatg atgaagaagt ctattcaatg gcagtggtaa
    24421 ataatatttt cggtggaagt ataagctcaa ggctttttca gaaaataagg gaagaaaaag
    24481 gtttagtata ttcaatatac tcatcacaga cattatatag gaaatgtggt gaattaggta
    24541 tatttgcaag catgagtaca gaaaatttac aagatgtata taatttaata aagaaagaaa
    24601 ttgaaaatat aagagaaaac tatcttacag aaaaagaaat atctgaaagc aaggaacagt
    24661 taaaaggaaa ttatatattg gatttagaaa gtactagcag tagaatgatg tctactggta
    24721 aatctatgtt acttagtaaa aaagtaaaaa ctacagatga aatacttgaa tgtataaata
    24781 gtgttaatat taattcaata aagaaggttg ttgataaggt attcaatatc gaaaatattg
    24841 gtacatgtat agttggaaga gatgtagaaa agattctaca cttggattaa tattgaaaaa
    24901 agtaaatctc tggaggtata aaatatgaat ttatctgaaa tagcaggtaa ggaaatagtt
    24961 aacttggtca caggagaaag attgggggtc attggagaat gtgaccttat actagatgaa
    25021 actacaggcg gtatattagc gcttctaatt ccaaaagaaa aaggattttt tgccagaaga
    25081 aaagataagt catttttaga agtaccatgg agaaatgtaa aaaaaatagg taatgacatg
    25141 ataattatag aatatgaggg atatatttag gaggaaaaaa tgagtatttt agtgcaaaag
    25201 tttggtggga catcagtaga atcatatgaa aaaatgaatg aagtatgtaa gatagtaaaa
    25261 gcatacaaaa aaaatgatga agaattacaa ttagtattag tagtttcagc aatgggaaga
    25321 aaaggagctc catatgctac agatacactt ataaatttgt gtagtgcagt aaatgatgag
    25381 ccaagtaaaa gagaacttga tttaataatg tcttgtggag aaataatatc aggaacaatt
    25441 cttgctaatt tattgaatgc acaggggata gattctgttt tctttactgg tcagcaagca
    25501 ggtataatta caagtgatga atattctaat gcaaagataa agtatataaa tcctaaaaag
    25561 gtaaaaagag ctttagatga tggtaaggtt gtagttatag ctgggttcca gggtgtaaca
    25621 gatgatggag aaataactac tcttggaaga ggcggaagcg atacatcagc agttgctatt
    25681 ggtaaagctt tagaatgtga aacagttgaa atttacactg atgtggatgg tataatgact
    25741 gcagacccta gagtagaacc aaacgctaaa gtattgagtt ttatagacta tgaggaagta
    25801 ttccaaatgg ctgacaaagg tgcaaaagta atacatccaa gagcagtaca gttggctaaa
    25861 agtggaaata taactttagc cataaagaat acaatgaatc caacttttga aggtacaaaa
    25921 ataggttctt tatgtagaca cttagaagac aatatagaat atgaacaaga aagagatttt
    25981 aaggttgctg ttgcaaataa agatagcgta gcacaagtta agattaagtc agcagaagaa
    26041 gtgtttacag aagtattaaa tgagatagaa aagaaactta taactataga tatgataaat
    26101 ttctttatat cagaaaaagc atttgttgta gaagatgctg atataaaatc attaaaggaa
    26161 atactagata aatttgaatt agattatgaa gtaaatagag actgtgctaa ggtaacattg
    26221 atatgttcaa gaattgctga tgaaatgtct ggtatcatgt caaaggtagt tagaggatta
    26281 tcaaaagcag gggtatccct tttacaaaca tcagactcaa atatgactat atcttgttta
    26341 gttagtgaag aagatatgca tacagcagta catgctatac atcaacaatt ttatttaaaa
    26401 taaactgtat aatacaatta ttttttaatc aaatttaaga atttcatata tatcttaata
    26461 tatttgattt atttggtaaa actaaattaa atataatttt gataggagtt tttttatgaa
    26521 agattataga gaagataatg aaagatttga taaagataat tgtagtgaag aatctagttg
    26581 cgaagagaga gaatgtataa agcaatttgg taccaatgaa atgccacctc aacctcctaa
    26641 agatattcaa tgtataacta taattggaga aattgaggga cattttatag gaaatcctca
    26701 aaaaaaagct acaaagtacg agcatataat tccaatgcta tactcaattg aggaaagtaa
    26761 tgatgttaaa ggagtgttag ttgttttaaa cactgttgga ggagatattg aagcaggttt
    26821 agctattgca gaacttttaa acagtacatc taaaaaagta gttactcttg ttctaggagg
    26881 aagtcatagt ataggtgttc ctcttgctac agcaggagat tactcattta tagcaccaac
    26941 agcaaccatg attatacatc cagtgcgaac tactggtctt gtgattggga taaatgagac
    27001 ttttgagtat tttaaaaaga tgcaagatag aataattcaa tttataataa gaacatcaaa
    27061 tatcaaaaaa gatgtacttg aaaaacttat gcatgaaaaa gatgaacttg taagtgatgt
    27121 tggtagtgta ttaataggaa aagaagctgt ggattatggt cttatagatg aagttggagg
    27181 actaaaagaa gcacttaaaa aattaagaga gcttataaaa gaatctgagg aagaaaaaaa
    27241 taatgattaa aatttgttta aataataaaa tttatgatat aatgatgtaa taaagtagga
    27301 gtgttagtgt taaggcatta gcactttttg ctgtgaaggg gtaaaacttg ctaaaaataa
    27361 ggagtgatat taatggccac gaaaaagaag aaaaaaaaga aggtaaatac cttaagtttt
    27421 aatgcagaat atcataattt aataacaata tttataggtg tatttttatt atatagttta
    27481 aattctaatt ctatgggatg gatacctgtg ttaatgcaaa atctttttaa gggactattt
    27541 ggagggcttt caatagcaat accatttata gtaattataa caggactttt aggatttttt
    27601 gatggaaatg aatatatata taggctaaga aagactaaac tatactacat aattatttta
    27661 tttatatttg tattttatgg attattaaat gcaggaactc ttcctgtaga tagcccacta
    27721 aaaggtaaca tgtttgatga tgttatgaaa ttgggtgtaa gtggacaagg tagtggtttg
    27781 atagcaacaa ctattgcgta ttatatgagt aaaatatttg gtatagcagg tggatggtta
    27841 ataagtatct tcgcacttat actttctgta atgtttatat ttaatatttc tataaaagat
    27901 ttggtgtcaa atgcaaaatc aaaatctaaa gcatctaaag acagcaattt aacgtttaaa
    27961 gataaaattg caaatatgaa aaaatctgca atagatatga tgactgatga agtagatgat
    28021 actactataa ataaaaaacc aggtttcttt aaagggttaa tgtcaaaagg tcttaataat
    28081 gatgatgaag aagatgaata ttttgaagaa gaaaatagtg atggtgtaga tgataaaact
    28141 ataaaaatag ttggatttaa taaggcagaa gatgagtatt tagaaatatt agaaggaact
    28201 caaagtatgc cagagttaga tgttttaaaa gaattacaaa aagctaccaa tgaaaatcca
    28261 gttgtagata ctaaaccaga aaaaaaagtt gatatagcta aacctaattt aaatatagaa
    28321 aaaactcaac ctatgagtat tgtagcagag ccagttaatg aggattattc aaactataaa
    28381 aagcctagca ttgagctttt aaataaagta aataagaagt ctgatgaaaa tggtaaaaag
    28441 aaagtgttga aaaatgcttc tcttttagaa aagacactat cagattttgg agtggaagca
    28501 aaaattaatc aagttacagt tggacctact ataacaagat atgaaataca accaagtcca
    28561 ggagtcaagg taagtaaaat tgtaaattta actgatgata tagctttaag tttagcagct
    28621 aaaagtataa gaattgaagc cccaattcct ggtaaaagtg caattggaat agaagttcca
    28681 aatgaagaag ctcaaatggt tggtgtgaga gaagttctag aaagtgaaga atttaataat
    28741 tttaactctc ctcttgcaat gggacttgga aaagacgtag caggaaaaat tataattgga
    28801 gatataggta agatgccaca cttactaata gctggttcta caggttcagg aaagagtgtg
    28861 tgtgtaaata cattaataag tagtatttta tataaggcaa atcctgatga agtaaaatta
    28921 ctattaatag acccaaaagt agttgaactt gcaaattata atggaatacc acatctattg
    28981 ataccagtag ttactgaccc taaaaaagct gcaaatgctt taaattgggc agttacagag
    29041 atgaatagaa ggtacaaatt atttgcagat gctcaagtaa aagatgtaac aagttataat
    29101 gagaaagcgg aagaaaaact tcctaaaata gtaataataa tagatgagtt agcagattta
    29161 atgatggcaa gtgcaaatga tgtagaagat tatatttgta gattagctca aatggcaaga
    29221 gctgctggaa tgcacttgat tgtagcaact caaagaccat ctgttgacgt tataacaggg
    29281 gtaataaagg ctaatatacc atctagaata gcatttgcag tatcatctca aactgattca
    29341 agaacaatcc ttgatatggg tggtgcagag aagcttctag gtaaaggtga tatgttattc
    29401 tatccacttg gtgcagcaaa accagttaga cttcaagggg cattcatatc agagagtgag
    29461 tcggaaaaag taatcgattt tgtaaaaagt caagttaaag atggaataaa atatgaagaa
    29521 gatataattg aaactatatc taaggttaat acttcaaagg gtagtgatga ggatgagttt
    29581 ttgagtgaag cgatagaatt tgtcgtggaa agtggtcaag catctgcctc tatgctacag
    29641 agaagattta aaataggatt caatagagca gctagactta tagattctat ggaagaaaga
    29701 ggaataattg gagcaagtga gggaagtaaa ccgagaaagg tattaataag caaacaggat
    29761 ttgcagaatt tagaaggtga atagaattgt cagttataaa aatagaaaaa gcacttaagc
    29821 tagataaagt aatatttgta gatgtgagaa ctgaaggaga atatgaagaa gaccatatat
    29881 tgaatgcaat caatatgcct ctttttaaaa ataatgaaca taatgacgtt ggaacaattt
    29941 ataagatgca gggaaaacat gaagcaatac aaaaagggtt tgactatgta tcgtataaat
    30001 taaaggatat atatttacaa gcagcagaac ttgcattaaa ttatgataat attgtcatat
    30061 attgtgcaag aggcggaatg agaagtggca gtatagtaaa tttattatca tcattaggtg
    30121 taaatgtata tcaacttgaa ggtggatata aggcttatag aaattttgta ttagagtatc
    30181 taaggcatgt aatggataca aaaaactttg tatctgttca tggattgact ggtacaggaa
    30241 agactgattt gttacattta ttagatgaaa aaggaataga tgtactggat ttagaaggca
    30301 tagctaaaaa cagtggttct acctttggtt ttataacttt tgataaaaag ccaccttctc
    30361 aaaagaactt tgaaacaaaa ctatttgaaa gtatattttt ttcgaaagag aattatatat
    30421 ttgtcgagag tgaaagcaaa cgagttggaa gtgttacagt tccaaatgaa attttcgagg
    30481 caatgacacg agaaggacat catatattat tagaatgtga tattgaaaat agagttgata
    30541 gactttgtag agattatatt tatgataaag atgaaggtaa tatattgatt ttaaaagaat
    30601 gtataaacaa gtttagaaaa agattgggta ataaagtagt agatgattat attgatttat
    30661 tagaatcagg tgaatataaa gaacttgtaa aaagatatct tttagaatac tacgacccac
    30721 tttatatgca ttcagtagaa aaatataagt atagtaagat tataaatttt aatgatacac
    30781 aaaaagcttt agaagatgtt atagacttat atgagagtat attggaagga gaaagtgaat
    30841 gttaaaaata gcattagaat cattaggatg ctcaaaaaat ttagtagatg cagaaataat
    30901 gatgggaatt ttaaataaca aaggctataa attaattgga gattttgaag aagctgatgt
    30961 aattatagta aatacatgtg gatttataga gtcagctaaa caagaatcta tagatacgat
    31021 aataaacttt gcagagttaa aaaaaactgg caatttaaag ttgttgatag taacagggtg
    31081 tctagcccaa agatattctg aagaattaaa aactgaaatt cctgaaatag atgctatagt
    31141 aggaacagga agctatcaaa atatagataa aatacttaaa gaattaagtg aaatgcataa
    31201 aattgtaagc cttaatgata tagagtttgt atttaatgaa gatttaccaa gatatatatc
    31261 tacacctagc tatatggcct atttaaaaat tggagaagga tgttccaata attgtactta
    31321 ttgtataata ccaaaactaa gaggtaaata tagaagtaga aaatttgaag atataattaa
    31381 agaagctaag aaattagctg aatctggagt aaaagaactt gtggttatag cacaggatac
    31441 gacaaagtac ggttttgatt tatatggaaa agaaagatta tcagaacttt tagaagaact
    31501 tgcaaaaata gatggattta agtggataag ggttatgtat tcatatccag aatctataac
    31561 agaagaattg atacaagtta taaagaaata tgataatata tgtagttact ttgatatgcc
    31621 aatacagcat gcaagtaaca atattttaaa attaatgaat agaaaaacta ctaaagaaga
    31681 tatattaaat aaaatcaatc tgataagaag taatatacca gatgctatac ttagaactac
    31741 aataatagta gggtttcctg gtgaaacaga agatgacttc aaacaacttg tggactttgt
    31801 tgaagaggtt aaatttgata gacttggtgc atttgcatat tcaagagaag aagatacacc
    31861 agcagataga ttgccaaacc atatagatga agaagtaaaa atacaaagaa gagatacttt
    31921 aatgatgatt caacaaaaaa tatctgaaga acttaatgac aaaaagatag gaaaaactta
    31981 tgaggtgtta atagaagaac agatagaaga taatgtatat acaggaagaa cacaaggtga
    32041 tgcagaggaa attgacagta tagtgtatgt aaaaagtgta gacaatcttg aagtaggaga
    32101 atttgtaagt gttcaaataa atgatgcaat ggaatacgat ttaatgggag atgttttata
    32161 tgaacttgcc taacaagtta actttgttta gaatattttt aataccagta tttgttttaa
    32221 ttatgttgct caatatacca aataaatttt taatagcttg tattattttt ataatagctt
    32281 caattactga tgctttagat ggaaaaatag ctagaaaata caatctagtc actgactttg
    32341 gaaaatttat ggacccatta gcagataagt tattggttat ttcagcactt acatgtatga
    32401 tagaagacca tcttgtaagt agttggatgg taattataat agtggctaga gagcttacag
    32461 ttagcatatt aagagctata gctgctgctg atggaaaagt aattgctgct ggaaacagtg
    32521 gaaaacttaa aacgataact caaatggtat ctatagtgtt tttattgtta ggtgctcagt
    32581 ttgaaaatgt attgatatta aatattggtg aaattctcat tcttatagct acactactta
    32641 cattatattc tggatgggaa tatctatata aaaataaaga gctttttatg tcttctaagt
    32701 aaaaaattat gctttaataa ataaaaacta atgattaata aataaaaaat aacaacttaa
    32761 aattttaaaa atgtaaaaaa gggtatacta ttatagtata cctcttttca ttttaagaat
    32821 attttctttt gtgtatataa ttattaataa tggtttataa ataaaatgta atgaataaaa
    32881 atataatcat tagtagcgtt tttaaattga ctaggtttga tttatgttat ataattaata
    32941 tataacaaga gaacaaatgt ttgtagataa atgaagggat ggtatagaga tgagtgtaga
    33001 tcaagaaaaa ttaaaagcgt tgaatgaagc tttaggtaaa attgaaaaag attttggtaa
    33061 aggttcagta atgaaattgg gagaagcaac atctatgtct atagatgtta tatcaacagg
    33121 agcgattggt ttagacatag ctattggtat aggaggtcta cctagaggga gaatagttga
    33181 agtatatggt ccagaatctt ctggtaagac tactgttgcg cttagttgtg tagcatcagc
    33241 tcaaaaagat ggaggaatag ctgcatttat agatgcagaa catgcacttg acccagtata
    33301 tgcaaaagct ttgggtgtgg atgttgataa cctaataata tctcaaccag atacaggtga
    33361 acaggcttta gagatagcag aggcattgat aagaagtgga gcgatagata taatagtaat
    33421 agactcagta gcagcattag ttccaaaggc tgaaatagat ggagatatgg gtgattctca
    33481 tgtaggttta caagctagac ttatgtcaca agcacttaga aagttaactg gttcaattaa
    33541 aaaatcaaat tgtgttgcta tatttataaa ccagttaaga gagaaagtag gaataatgtt
    33601 tggaaaccca gaaactacta ctggaggacg tgcactaaaa ttctattcat cagttagatt
    33661 ggatgttaga aaaatagata caataaaaca aggtgataaa gttataggta gtagaactag
    33721 agttaaagtt gttaaaaaca aagtagcacc accatttaag caagctgaat ttgatataat
    33781 gtatggagaa gggatttcaa aaattggaga ccttttagat atagctgctg atgtagatat
    33841 agttaaaaaa tcaggttcat ggtatagtta caatgatact aaacttggac aaggaagaga
    33901 aaatgttaaa aaattcttgg aagataattt agatttaact actgaaatag atgagaaagt
    33961 tagagcattt tacaatttaa atgaagaaca tgaagaatca ggtacttcag tatcaaaaga
    34021 aattgtagaa gaataagtaa aaatacaaaa aaataactag taattaaaga catgccacgc
    34081 tccctattag cttgacacaa tgttgaaaaa attataaaat taagtgagga taaaatatct
    34141 aaatttaatt ttaattatta ggaatatttt ttaatcatag tgatgacatt ttgggattat
    34201 taacgaagga ggtggatgtc atagatagtg tagtaatgat tgtaataggt gcagctgttg
    34261 gtattatagc tggatatttt gttagaaaaa atatatctga agcaaaaatt ggacaagctg
    34321 aaaacttggc caaagaaata atagataaag cacaccacga ctcagaaaca gtacaaaaag
    34381 aaaaattact agaagctaaa gaagagatcc ataagtggag aacagaagct gaaagagaaa
    34441 acagagaaag aagaacagaa gtacaaaagt atgaaaaaag agtagtacaa aaagaagaag
    34501 tcttagatag aaaattacaa aatttagaat ctaaagaagt aaacctaagt gaaaaattaa
    34561 aggtagtgga aaagaaagaa gaagaagttg aagtaataaa aactcaacag ttagaaaaat
    34621 tagaaagtat atcaggaatt acttctgata aggccaaaga aatcatctta accaatgcgg
    34681 agagagatgt aagacgtgaa atgtctataa tgataaaaga gatagaaagt caagctaaag
    34741 aagaagctga taagaaatca agagaaatta taggatatgc aattcaaaaa tgtgcagcag
    34801 atcacgtagc cgaaactact gtaacagtag taagcttgcc taatgatgaa atgaaaggta
    34861 gaataatagg tagggaaggt agaaatataa ggactttaga aaccttaaca ggtatagacc
    34921 ttataataga tgacactcca gaagccgtaa ttttatcagg atttgatcca ataagaagag
    34981 aaattgcaag aattgctcta gaaaaattaa tagcagatgg aagaatacat ccagctagaa
    35041 ttgaagaaat ggttgaaaag gcgagaaaag aagttgacaa tattataaaa gaatatggtg
    35101 aacaagctgc atttgaaact agtgtacatg ggattcatcc ggaattaata agattattag
    35161 gtagacttaa ctatagaact agttatggac aaaatgtact taaacattct atagaagttg
    35221 ctcatatagc tggtataatg gctgctgaaa taggagcaga tattagattg gctaagaggg
    35281 caggtttatt acatgatata ggtaaggctg tagaccatga aatggaaggt acacatgttg
    35341 aaataggtat ggatttactt agacgttata aagagtctaa agaagttata catgccatga
    35401 gtactcatca tggagattac gagcctcaaa cagtagaggc tgtattagtt acagcagcag
    35461 atgcaatatc agctgctaga cctggtgcga gaagagaaac tttagaagca tatataaaga
    35521 gacttgaaaa attagaagaa atagctaatt catatgaagg tgtagaaaaa tcatttgcta
    35581 tacaagctgg tagagaaata agaataatgg taaaaccaga agctataaat gatgaagaaa
    35641 tacatctatt ggctagagat atgactaaga aaatagagga tgaacttgaa tatcctggac
    35701 aaataaaagt aagcataata agagaaacaa gagctataga gtatgcgaag taaaagaaat
    35761 aatatctaaa taataagaaa atatttttag aaatttaaat ttgccacaag atatgataat
    35821 atatcttgtg gcttttaaat tgtttaagtt taattttata taatgcaaaa ataatctaag
    35881 tgctaaaagt gctacttaca aaagattaat aaaataaagc tttatatatg tattatttat
    35941 ataattatcc aagtttgttt gatataatga agttgtaaaa taaatatatt actacttgga
    36001 atgcttgtac ttaaataaga tgaaaataca atgacgctaa agtgaggatt agagagaggg
    36061 tacactatga aaaaaaatac acttttacat acaaatatta tggtttgcat tattattact
    36121 cttggttttg tggctacctc tataattgga tatcaatcta ataccaatat ttttaaaaat
    36181 gatattgaac atgtatctac attggctgct gaaggtattt attatcagat agataaacta
    36241 ctttcagaac ctataaatgt atctcttaca atggcaaatg acagtctatt aaaaaatttt
    36301 ttagatggtg agaaagaaca tttaaatgat gaagaattta tttataaatt acaagattat
    36361 ctgaatgtgt atagaaataa atattcatat gattctgttt ttttagtatc aactaaaacc
    36421 aataattatt atcattttaa tggacttgat agaaatctta gcgctaataa ctcagagaat
    36481 caatggtatt atactttctt aaagaatgat gatgaatact cattaaatgt tgataatgat
    36541 gaagcaagta acaatagtat tacagtcttt gtaaattgta agataaaaga tgataatgga
    36601 gcaactatgg gaattatagg tgttgggtta aaggttaatt cattacaaat gttgcttaaa
    36661 ggatataatg acaaatttga tgttgttgca cgcttaattg atgataaagg gtttgtacaa
    36721 ttagcagtag ataaaaccgg tcatgagaat ataaattttt ttgaaaattc tagtagcgac
    36781 ttgtctaatt cgaaacaatt aatattaaac aacaaaaagg aacaaaagag tttttggtat
    36841 tcatctgaaa aatcaaaaag ttatattgta tgtcagtaca tacctagttt aaaatggcat
    36901 cttgttctag aaaatgattt tactttaatg attaaacagc ttcatctgca attttttaaa
    36961 ggcattgcag ttatagtttt aattataata tttgtacttt ttactattac acatgtgctt
    37021 aaaaaataca aaaaccaaat aataaagctt agtgtttcac agaaattgga atatcaaaaa
    37081 ttgttgagca aatctactga aggtctttat gaaaatattt ttgaaattga tattactaac
    37141 aataaagctg gttgtgaaga tgagaatagg tattttcatg aatttggatt agggcttaat
    37201 accccatatg atgaagcctt acatacaata gctaaaaacc aaatcaaaga agaatatgca
    37261 caaggttatt tagatacatt tttacctgaa aatgtaatta aacgttataa tagcggaatt
    37321 aataatatca gctatgattt tttaattaaa aaagatgaag aaagttatca ttggattaga
    37381 attaatgcac gaattttctt ttggacatca gatgaatctg ttcgtatgat atcttatcgt
    37441 aaaaatatag atgaagaaaa aaatagagaa ttactacttc tcgaacgttc acaacgtgat
    37501 tctcttacag ggctttacaa taaagctaca acagaggaat ttattcgttc atttttaaaa
    37561 aatgaaagta atcaagattt agaacacgtt tttctaattt tcgatattga taattttaaa
    37621 aatgtaaatg ataaatatgg tcatgtattt ggagactttg ttataagtga atttgcctta
    37681 gaattaaaat ctcaatttcg tgaggacgat attgtgggac gtattggtgg agatgagttt
    37741 gtagtattaa taaaagactt taataataat aactatgtgc ttatagacaa attagagcgt
    37801 ttttgttcta agattaatca gaaaaaattt gttgagcaag cagaattttc tattgcatgt
    37861 agtattggtg tagcaatgta tcctgaacat ggatatgtat actctgaact atatgagaaa
    37921 gcagaccaag cactttacta tgcaaaaagc catgggaaaa attcttatca tatattttca
    37981 gaggattcta ttgatagtat ttcatttcat atagactcta gagatataaa agtattatta
    38041 gaaatggcta ctgatggtgt tgtcaaattt gcttgtatgg aaaacttaaa attactttat
    38101 ttcaaccaaa aaatgcttga atttactgga tattcagctc aaactgttac ttctataggt
    38161 tttgatattt tttcgcctgt attacaagac gacttacaaa tggtactaga tacattaaga
    38221 gaagcaattt ctaaaagaga gacttttgaa atagtttttc gtttttgtca tacagatggt
    38281 catatttttc ctgtaaggat gcaaggtaag tttgtaagtg aattatatca aaatcgttat
    38341 cctgtttttt atgcgattta tacagacata tcaagtatca atactttact agaaaataaa
    38401 tagtaaatac tacagtaaaa
//
LOCUS       DAMJYX010000026        37478 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJYX010000026 DAMJYX010000000
VERSION     DAMJYX010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.026"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            12..410
                     /gene="tnpA"
                     /locus_tag="PC479_001475"
     CDS             12..410
                     /gene="tnpA"
                     /locus_tag="PC479_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2804024.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            407..1570
                     /locus_tag="PC479_001476"
     CDS             407..1570
                     /locus_tag="PC479_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2804025.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(1954..2466)
                     /locus_tag="PC479_001477"
     CDS             complement(1954..2466)
                     /locus_tag="PC479_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2804026.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            2916..3392
                     /gene="purE"
                     /locus_tag="PC479_001478"
     CDS             2916..3392
                     /gene="purE"
                     /locus_tag="PC479_001478"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2804027.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            3398..4096
                     /locus_tag="PC479_001479"
     CDS             3398..4096
                     /locus_tag="PC479_001479"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2804028.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            4108..5475
                     /gene="purF"
                     /locus_tag="PC479_001480"
     CDS             4108..5475
                     /gene="purF"
                     /locus_tag="PC479_001480"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2804029.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            5469..6545
                     /locus_tag="PC479_001481"
     CDS             5469..6545
                     /locus_tag="PC479_001481"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2804030.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            6539..7132
                     /locus_tag="PC479_001482"
     CDS             6539..7132
                     /locus_tag="PC479_001482"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2804031.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            7125..8657
                     /gene="purH"
                     /locus_tag="PC479_001483"
     CDS             7125..8657
                     /gene="purH"
                     /locus_tag="PC479_001483"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2804032.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            8668..9918
                     /gene="purD"
                     /locus_tag="PC479_001484"
     CDS             8668..9918
                     /gene="purD"
                     /locus_tag="PC479_001484"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2804033.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            9943..13749
                     /locus_tag="PC479_001485"
     CDS             9943..13749
                     /locus_tag="PC479_001485"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2804034.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            13955..14827
                     /gene="rfbD"
                     /locus_tag="PC479_001486"
     CDS             13955..14827
                     /gene="rfbD"
                     /locus_tag="PC479_001486"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2804035.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            14936..15805
                     /gene="rfbA"
                     /locus_tag="PC479_001487"
     CDS             14936..15805
                     /gene="rfbA"
                     /locus_tag="PC479_001487"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2804036.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            15854..16411
                     /gene="rfbC"
                     /locus_tag="PC479_001488"
     CDS             15854..16411
                     /gene="rfbC"
                     /locus_tag="PC479_001488"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2804037.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            16449..17432
                     /gene="rfbB"
                     /locus_tag="PC479_001489"
     CDS             16449..17432
                     /gene="rfbB"
                     /locus_tag="PC479_001489"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2804038.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            17462..18130
                     /locus_tag="PC479_001490"
     CDS             17462..18130
                     /locus_tag="PC479_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2804039.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            18139..18567
                     /locus_tag="PC479_001491"
     CDS             18139..18567
                     /locus_tag="PC479_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2804040.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            18591..18905
                     /locus_tag="PC479_001492"
     CDS             18591..18905
                     /locus_tag="PC479_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2804041.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            19056..19331
                     /gene="flgM"
                     /locus_tag="PC479_001493"
     CDS             19056..19331
                     /gene="flgM"
                     /locus_tag="PC479_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2804042.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            19336..19752
                     /locus_tag="PC479_001494"
     CDS             19336..19752
                     /locus_tag="PC479_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2804043.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            19770..21080
                     /gene="flgK"
                     /locus_tag="PC479_001495"
     CDS             19770..21080
                     /gene="flgK"
                     /locus_tag="PC479_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2804044.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            21099..22031
                     /gene="flgL"
                     /locus_tag="PC479_001496"
     CDS             21099..22031
                     /gene="flgL"
                     /locus_tag="PC479_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2804045.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            22047..22439
                     /locus_tag="PC479_001497"
     CDS             22047..22439
                     /locus_tag="PC479_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2804046.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            22433..22645
                     /gene="csrA"
                     /locus_tag="PC479_001498"
     CDS             22433..22645
                     /gene="csrA"
                     /locus_tag="PC479_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2804047.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            22667..23062
                     /gene="fliS"
                     /locus_tag="PC479_001499"
     CDS             22667..23062
                     /gene="fliS"
                     /locus_tag="PC479_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2804048.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            23062..23418
                     /gene="fliS"
                     /locus_tag="PC479_001500"
     CDS             23062..23418
                     /gene="fliS"
                     /locus_tag="PC479_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2804049.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            23439..24962
                     /gene="fliD"
                     /locus_tag="PC479_001501"
     CDS             23439..24962
                     /gene="fliD"
                     /locus_tag="PC479_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2804050.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            24965..25300
                     /gene="fliT"
                     /locus_tag="PC479_001502"
     CDS             24965..25300
                     /gene="fliT"
                     /locus_tag="PC479_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2804051.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            25402..26274
                     /locus_tag="PC479_001503"
     CDS             25402..26274
                     /locus_tag="PC479_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2804052.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            26367..28508
                     /locus_tag="PC479_001504"
     CDS             26367..28508
                     /locus_tag="PC479_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2804053.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            28532..30409
                     /locus_tag="PC479_001505"
     CDS             28532..30409
                     /locus_tag="PC479_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2804054.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            30402..32516
                     /locus_tag="PC479_001506"
     CDS             30402..32516
                     /locus_tag="PC479_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2804055.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYSSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            32525..33520
                     /locus_tag="PC479_001507"
     CDS             32525..33520
                     /locus_tag="PC479_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2804056.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            33612..34844
                     /locus_tag="PC479_001508"
     CDS             33612..34844
                     /locus_tag="PC479_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2804057.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            34864..35808
                     /locus_tag="PC479_001509"
     CDS             34864..35808
                     /locus_tag="PC479_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2804058.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            35902..36309
                     /locus_tag="PC479_001510"
     CDS             35902..36309
                     /locus_tag="PC479_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2804059.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            36396..>37478
                     /locus_tag="PC479_001511"
     CDS             36396..>37478
                     /locus_tag="PC479_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2804060.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIIN"
BASE COUNT        15376 a         3780 c         6830 g        11492 t
ORIGIN      
        1 tgttagtttt tatgtataaa tctaacaaaa atatagttta tgattgcaaa tatcatattg
       61 tattttgccc taagtatcgt aaacctgttt tagttggcga agttgaaaag atgttaaaag
      121 aaatactacc atataaagca gatgaacttg gagcagaaat aatagaaatg gaaactgata
      181 aagaacatgt gcacttatta atatcttgtg accctcaata tggaattcat aaagtagtaa
      241 aaggtttaaa aggctttagt tcgagagtat taagagagaa ctttccacat ttaaagtcta
      301 gtatgcctag catgtggact aatagctatt ttgtaggtac tgttggttct gtgagtttag
      361 aagtagttaa acaatatata gaaaatcaac aagttaggac aaagaaatga taaaaactca
      421 tagagtgaaa ttaaacctaa ctagaacaca atttgaatta gtaagggaaa agcaaatgga
      481 aagtgctaac tgttggaact acatagttaa cttatctaaa gaatactatt ttgaacataa
      541 acaatggatt aaaaagaatg atatacaaaa gttaatcaaa ggtaagtaca atcttcatag
      601 tcaaactatt caagctatat cagataaatt tgatgcaaat agaaagacta tatcagaatt
      661 aagaaaaaaa ggtaatacta aagcaaaata tccatacaag actaagaaat tttatataat
      721 accttttaaa gctagtgcta ttaatagaaa tgctaaaggc aacttaaaat tatcaatgtc
      781 aaaaggcagg tatttagaat tagattttaa tgttgagaat ataaaaactg ctgaaatagt
      841 atggcgaaat ggatattatt tatactacac ttttgacaat gaattaaatg gagttgtagc
      901 caaaggcact aataccgtag gtgtagactt aggggaaata cattcgatag ctagtgtaac
      961 aaataaaggt gtagggctta ttctatctaa tagagaaggc agaagtatta aacaatttag
     1021 aaataagatg tatgcttata tatctaaaag attaagtaaa tgtaaaaaag gtagtagaca
     1081 atcaaagaag ttatggagat taaaaaataa gataagaagt aaaacagata atcagttaat
     1141 gaatttatat catcaaacaa ctagaaagtt tatagacttt tgtgtagagc agaaggtatg
     1201 tgaaatagtt cttggggata taaaaggggt tgaaaaagat actaagaaaa agaaaagatt
     1261 aaacagagtt aatagacaga aaatatctca aatggagtat ggtcgaataa aggactatat
     1321 aaaatataaa gcaaaagagc aaggtattga agttaagtta gttaaagaaa actacacttc
     1381 tcaaacttgc ccaaagtgtt ctaaaaaaca caaaccaact ggtagaactt atagttgtac
     1441 ttgtggttat gaaactcata gagatatagt tggtgcttgg aatattttaa ataaaaagca
     1501 taaatatggt ttagtagatt ttagaataaa tcataagcaa ccaataaatt taaaagtatc
     1561 taccgtttga gccatgtgtg ggcgaataac tttaaggtag tagtggcgaa gacaccgtcc
     1621 gaagtagctt ggctttgtaa ccacctctgt tgtactgttt aagtataaat aagtgtgtta
     1681 gccaccaaga atccccttgc tttagctatg gggagtgtgt caataaaata ctttaatgaa
     1741 aaatagctaa ctttaattgt aggatattct taatatattt gaaataaata aaaatattga
     1801 aggatataat attcaactgg tatataaata ataaatgata gttaaatttt accttagagg
     1861 ctaattaggt gtttagtcaa agctaatgcc tttagaatac aaatgtattc taaaggcatt
     1921 tttactatgt attttcattt atattactaa acattatcta tttttatata catttctatg
     1981 tattttattt tcatcaaatc ggtctataaa ctcatttttt tcttcactat atccaataga
     2041 tataagtgca taaggaacta attttaatgg taagtctaaa gtttgtctaa ctttatttac
     2101 cactttattt ttaggaaata ctcctgccca acaagcagct agaccttgat tagtaacttc
     2161 taataataaa ttttgtgtgc aagcacctaa atcttgtatc catttaccag gttttaagaa
     2221 tttatctcta tttccaagca ctactataca taatggtgca aattctatat gctttgctct
     2281 atgctgtgac tttgagagct gtttcagcag ttccttatca tctactataa taaattccca
     2341 aggttgagca ttttttgatg aaggtgcttg catagctgat tttagtattt cagttatttt
     2401 atcttcttct atatttttat ctatatatgt tcttattgaa cgtctatttt ttataacttc
     2461 taacatacag attctccttt aaataaaatt atttctattt atataatatc attactttaa
     2521 attttagcta ttacttatat aaagaataaa ctttgtgttg gttttatcta agtaaaagta
     2581 acattcagaa ctaaaaaaca agactataaa tccaaagtat taattaaaaa agaagtaata
     2641 aaaaataata tacaagattt aaaaaagagt attgggctta aaatttaatt agaaaaccaa
     2701 ttttgagata atctgttttt tattttaaca aaaaaacata aataaagttc gatacgtagc
     2761 gaatgaaaaa attacaagaa atattttttt taaaaaattt caaaaaagag ttgataatat
     2821 atgtatatta atataaaatt taaggtgtat aattcgtata ataatacgaa tgatttgtag
     2881 tgtaaaataa cttataaacg gagaggggaa gtctaatgaa agtagcagta gttatgggtt
     2941 caaaatcaga ttatccaaaa ttagaagaag ggataaaact gcttgaaaaa tatggaatag
     3001 aagttgtagc aagagcatta tcagctcata gaacaccaga acaactttca atattcctaa
     3061 aagaaataga agatgatact gatgtaataa tagcagcagc aggaaaagca gcacacttac
     3121 caggtgtaat agcttcacaa acattaattc ctgtaatagg attacctatt aaatcatcaa
     3181 ctatggatgg actagactca cttttatcaa tagttcaaat gccaaaagga atcccagttg
     3241 caacagttac aatagactta ggtttaaacg cagctttatt ggcattacaa ataatgactt
     3301 taaaataccc aaaattaaaa gaggatttga aatcatatag agaagagatg gcacaaaagg
     3361 tgttagaaga cgacaataat ttaagggggt agcagaaatg ttattatatg aaggaaaagc
     3421 aaaacaagta tattctacag ataatgaaaa tgaatatgtt gtttattata aagatgatgc
     3481 aacagcattt aatggagaaa aaaaagcaga aatatcttca aaaggtatac taaataataa
     3541 gatatcaaca ataatctttg aaatgttaaa agaaaataat ataaatactc actttataaa
     3601 aagtttatca gatagagaaa tgttagttaa aaaagtagag attttaccat tagaagtaat
     3661 agtaagaaat attgctgctg gttctatctg taaaagagtt ggtcttgaag aaggtgtagt
     3721 tttcgatgag ccaatttttg aaataagcta caaaaatgat gcttatggtg acccaatgct
     3781 aaatgatgat tatgcagtgg caatgaagtt agcaactaga gaagagttaa aattcttaag
     3841 agaagaaact ttaaaaataa atgagttact aaaagcattt ttcttaaaat taaacctaaa
     3901 gttagttgac tttaaaatag aatttggtaa ggattctgaa ggaaatataa tattggcaga
     3961 tgaagtatca ccagacactt gtagactttg ggatgtaaat acaaatgaaa agttagataa
     4021 ggacagattt agaaaagacc ttggagacct tgtagaaggt tatacggaag tactttcaag
     4081 aatgaataat aaataggagg caaatgtatg tgtggagttt tagggattta ctctaataaa
     4141 gatgtaacta aagaactata ttattcttta tattctatgc aacacagggg gcaagaaagc
     4201 tgtggtttag ctcttttaga tgatggagaa attaagtaca aaaaagatat gggattagtt
     4261 ggagatgttt ttaaggaaaa tgaattatct aagttaaaag gaaatatagg gattggacat
     4321 gttagatatt caacagctgg aggaagtcat gtttctaatt gtcaaccttt agttggaagt
     4381 tgtagaaaaa gacaattagc catagctcat aatggaaatc tagttaatgc aaattattta
     4441 aaagatatgc tagaagaaga tggatatatg ttccaaacta attcagatac agaagtgatt
     4501 ttatatatac ttgctagata ttacaaaggg gatatagtag aaagtttaaa agtgacaatg
     4561 gattatataa aaggtgcata tgctcttgta ataatgagtc aagaagaatt ggttgcagtt
     4621 agagacccac atggattcag acctcttgta cttggtaaaa agggtgatga atatatattt
     4681 gcatcagaaa attgtgcaat agatatactt ggcggagaag ttataagaga tgtagagcca
     4741 ggagagataa tagtagtcaa agatggagaa ctaaaatcat atttttattc agaaaattat
     4801 aaaccagtta agaaaagttg tatatttgaa cacatatact ttgcaagaaa tgatgcaact
     4861 atagacaatg taaatgcata tgaatttaga attaaatgtg gtgagagatt agctcagaat
     4921 gaaactgtaa aggcagatat ggtagttcca gttccagatt caggttggcc aggagctatt
     4981 ggttatgcaa atgctagtgg attaaaaata agtgaaggct tagttaaaaa tagatatgta
     5041 ggaagaacat ttataaaacc tacacaagaa gaaagagaaa tagcagtaaa aataaaatta
     5101 aatcctcttt caacaataat aaaaggaaaa tctataattt tagtagacga ctcaattgta
     5161 agaggaacta catctaaaca attagtaaaa tctttaagag aagcaggtgc aaaagaaatt
     5221 catcttagaa taacttctcc tccagtagct tactcttgtt actatggaat agatactcca
     5281 aatcgctcta aattaattgc atcaagtaac aacgtagaag aaatgagaga atatattgga
     5341 tgtgatagtt taaaattcct agatatagaa ggaatgttag atgcaacaga acataagtct
     5401 actttctgta aagcttgttt tgatggagaa tacccagtta agaaaattga taaggaggaa
     5461 ctactttcat gttaacatat aaagaatcag gtgtagatat agatgaagga aatagagcag
     5521 tagaccttat aaaaggtaaa ataaaaggaa cttatgatgg aaatgtagtt ggagatttag
     5581 gtaacttcag tggattatac agtttaaaag attttgtagg tatgaaagaa ccagttttgc
     5641 ttgcatctac agatggtgtt gggactaagt taaaaatagc tcagatgatg gacaagcatg
     5701 atactgttgg aatagattta gttgcaatgt gcgtgaatga tttaatttgt caaggagcaa
     5761 aaccattatt cttccttgac tatatagctc ttgggaaatt agtgcctgag catatagaaa
     5821 aaatagttgg tggaattgca gatggttgca aaatgtctgg ttgtgcacta attggtggag
     5881 aaactgctga aatgcctgga atgtatggag aagatgacta tgatttagca ggattttctg
     5941 tgggaatagc agacaaagaa aagattgttt ctggaaacaa tgttaaatca ggagatgtat
     6001 tggttggtat atcttcaagt ggagtacaca gcaatggatt ttcttttata agaaagatat
     6061 ttttagaaac ttacaattat aaaatggaac gatatgttga agaactagga atgacagttg
     6121 gagaagcact tttaacacca actaaaatat atgttaaatt agctttagat gtattagcta
     6181 aacatgacat aaaagctgta gctcacataa ctggtggagg gcttatagaa aatataacaa
     6241 gagttatacc taaaggatta ggactagata tcaataaaaa atcttgggag aagcctccta
     6301 tatttaaaat gatagaaggt tttaatgctg ttgatgagag agaacttcat aagagtttta
     6361 acatgggtat tgggctagtt ttaatagtag ataaagaaaa tgctgatgat gttgtaaact
     6421 ttataaataa tagagaaaat gacaatgctg attatgtaga taaaaaatat agtgaattat
     6481 tagaagataa agcttatata attggtgaag tagtagatag tcatgaaggt gtagaattat
     6541 gctaaatata ggagttttaa tatctggtgg gggaacaaat ttacaagcag ttatagatgg
     6601 tactgagtct ggagaaataa aaggtcaggt taaagtagtt atctcaagta agcaaggtgc
     6661 ttatgggcta gagagagcta aaaatcataa cataaaagca atttgtgaaa ctgatgaaga
     6721 caagataata gaaattctta aggaaaataa aatagattta gtagttttgg ctggttattt
     6781 aaaaataatc agcccaaagc tggttaatga gtttagaaac aaaatgataa acatacatcc
     6841 atcattaatt ccttcatttt gtggagctgg attctatgga gaaaaagttc atcaaggtgt
     6901 tatagattac ggagctaaag taactggtgc tacagttcat tttgttgatg aaggtgctga
     6961 tactggacca atcataatgc aagatgtagt taaggtaaat caagatgatg atgcaaagac
     7021 acttgcaaaa agagtgttag aagttgaaca taggatttta aaagaaagta taagtctttt
     7081 ttgtgaaaat aaattaaaac ttcaaggtag gagagtgttt ataaatgagt aagagagctt
     7141 taataagtgt aactgacaag actggtgtag tggaatttgc taaagagtta aataaattag
     7201 attatgaaat tatatctaca ggaaatacat ttaaaacatt aaaagaaaat ggtgttaatg
     7261 taatgcaagt tgaagatgtt acaaattttc cagaaatatt agatggaaga gtaaaaacat
     7321 taaatccata tatacatggt ggtatacttt ataaaagaga taaagaatct catgtagaaa
     7381 ctgtaaatga acataaaata cattcaatag atttagtagc tgtaaatcta tatgattttg
     7441 aaggaacttt aaaagctgga aaaagtcatg atgaaataat tgaaaatata gatattggtg
     7501 gaccatctat gatacgttct gctgccaaaa actataagga tgtaatagta gtagtagata
     7561 taaaagatta tgattctata atagaaaaat taaaaacaga tacaatgaca ttagaagaca
     7621 gaaaaaaatt atcatataaa gctttctcaa ctacaggaag atatgatgcg ttaatatcta
     7681 gttattttgc tggagaagtt ggagatacgt atcctgacat attaaattta actttccaaa
     7741 aagaacaaac tttaagatat ggagaaaatc cacatcaaaa tggtttctta tattcacagt
     7801 caaatgcaaa aaatccaatc ttaaattatg agcaattagg cggtaaagaa ctttctttta
     7861 acaacttaaa tgatttacat ggatgtttag aagtaatgag agaatttaaa gatagtgaag
     7921 aagtagtttc agtagctata aaacatgcta attcatgtgg ggtaggttta ggaaaagatg
     7981 cttttgaagc atatacaaaa tgttatgaag cggataaggt ttcaatattt ggaggaatag
     8041 taggaataac ttctactata gataaagcta cagcagagaa attaaatgaa atattcttag
     8101 aaatagtagt agcatatgat tttgaaccag aagctttaga gatacttaaa caaaagaaaa
     8161 atctaagaat attaaaatta gctaaaatag aaaatagttt acaaccatat gaaatgaaat
     8221 atttagatgg aaaattattg atacaagata gaaacaatat attagcagaa aaaagtgaaa
     8281 atgtaacaaa ggaaaaacca acagatgcac aacttaagga catggaattt ggtatgagag
     8341 tagtaaaaaa tatgaaatca aatgcaattg caatagtaaa aaatggacaa actctagctt
     8401 taggttgtgg acaaacatca agaatttggg cattaaaaaa tgctcttgaa aataacaaag
     8461 ataaagattt tacaggagct gttttagcgt cagacgcatt tttcccattt gatgattgtg
     8521 taacattagc tcatgaatat ggaatatcag cagtagttca accaggtggc tctataaaag
     8581 ataaagattc aatagaagct tgtgacaagt atgatatggt tatggtattt acaggaataa
     8641 gacacttcaa acactaagga ggatattatg aagatattgg ttgtaggcgg tggcggaaga
     8701 gagcatgcta tatgctggaa actaagcaaa gaaaaaaatg tagaaaaaat ttattgtgca
     8761 ccaggtaatg caggaattgc aaacgtagca gaatgtgtaa atataggtga tactaatata
     8821 gaagaacttt taaaatttgc caaagaaaat gaaataggtc tgacaatagt aggaccagaa
     8881 gtaccactag ttatgggaat agtagatgag tttgaaaaag aaggattaag agtatttgga
     8941 cctaataaaa aatgtgctca acttgaagga agtaaagctt tttcaaaaga atttatgata
     9001 aaacataaca tacctactgc aaaatataaa gaatatacaa acttagaaga agctatatca
     9061 gaaatagata gttttggata tccagtagta ataaaagcgg acggattagc tgctggaaaa
     9121 ggtgttgtaa tacctgaaaa tagagaagat gctatagcaa ctttaaaaga aatgatgtca
     9181 gataagaagt ttggagctgc tggagataaa atagtaattg aagagttttt aaaaggaata
     9241 gaaacttcaa ttctagcatt tgtagataat gatacaatag ttcctatggc aagtgcaaaa
     9301 gaccataaaa aagtaaacaa ctatgagcaa ggtccaaaca ctgggggaat gggaactttt
     9361 tcacctagtg aaatatatac agaagaatta gcaaataaag taaaagagac agtattagaa
     9421 aaaactttag aaggcttcaa aaaagatgga cttaatttta aaggtatttt atttgttggt
     9481 cttatgataa ctgaagatgg agaaaaagta cttgaatata atgtaagatt tggagaccct
     9541 gagacacaat ctgttttatt tagattagaa acagatttac atgagataat ggaagctata
     9601 ctagataata agttaaaaga tatagaaata aactatagtg atgatgaagc tgtatgtgtt
     9661 atgcttacat caggagggta tccagacagt tatgaaaaag ggaaaattat aactggtctt
     9721 gaaaacttgg atgatgatat agtagtattc catagtggca ctaagatgtt tgatggtaat
     9781 ttggttacca atggaggaag agtaattggt ataactgcaa agtcaactac agttaaggat
     9841 gcagcagaaa aagtttatga aaacataaag aagataaact ttgaaggaat gcactataga
     9901 acagacatag gaagataatt tttctaaaat ggaggataag gtatgttgaa tactgaaaat
     9961 aaagactcaa tggttagaag agtattagtt gaaaaaagag aaggttttga ccttgaggct
    10021 aaagctttaa aaaaagactt agtagaaagt ttacacatag ataatataga aaatttaaga
    10081 atattaaata gatatgatgt agaaggaata tcagaagaag tttatgaaaa tgcagcaaag
    10141 acaatattct ctgaaccaaa tttagatgta gtatattatg aagaaatacc aaaattaaat
    10201 gatgaaagag tatttgcaat agaatttctt cctggtcaat acgaccaaag aggtgattgg
    10261 gcagctcaat gtgtacaaat tgtaaatcaa gggataagac ctgctattaa tacagcaaaa
    10321 gtttatatat tatcaggcaa aataactgat gaagagtttt ctaaaataaa agattactgt
    10381 ataaatccag tagatagtag agaagcatct ttagaaaaac cagaaacgtt aaaaatggaa
    10441 actgaaatac caacaacagt tgaagtatta gatggattta tagatttaga tgaaaatgga
    10501 ttaaaaacat ttgtaagtga aaaaggatta gcaatgactt taggagactt acaacacgtt
    10561 caaaaatact ttaaagatac agaaaagaga aatccaacta taacagaaat aaaagtatta
    10621 gatacttatt ggtctgatca ttgtagacat actactttta tgactgaaat agaaaatgta
    10681 aagatagaag atggtaagtt taatgatata gtaaaagaag cgtatcaaat gtatcttaac
    10741 tcaagagaca atgtatatgt gaacagacat aaagatatat gtttaatgga tatagccact
    10801 gttgcagtaa aagaattaaa gaaaaatggg aaattaaatg acctagatga aagtgaagaa
    10861 ataaatgcat gtagtataaa cgtagatgtt gaagttgatg gtaagatgga aaaatatcta
    10921 gtaatgttta aaaatgaaac tcacaaccat ccaactgaaa tagaaccatt tggtggagca
    10981 gctacttgtt taggaggagc tataagagac ccattatcag gaagaagtta tgtttatcaa
    11041 gcaatgcgtg ttacaggaag tgctgaccca agaacgactt tagaagatac tttacctgga
    11101 aaacttatgc aaagaaaaat aactacagaa gctgctcatg gatatagttc atatggtaac
    11161 caaataggtc ttacaactgg acaagttgca gaagtatatg atgaaaactt tgttgcaaag
    11221 agaatggaaa taggtgcagt tatagcagca gcaccaaaag agaatgtagt aagagaaaga
    11281 cctgaagcag gagatgtaat cgttcttctt ggaggaaaaa caggaagaga tggctgtgga
    11341 ggagcaactg gttcatctaa agaacatagt gaagagtcaa tcttaacttg tagtgcagaa
    11401 gttcaaaaag gagatgctcc taacgaaaga aaaatacaaa gattctttag aaataaagaa
    11461 gttgctcaaa tgataaaaag atgtaatgat tttggagcag gtggagtttg tgttgctata
    11521 ggagagattg ctgatagctt agatataaac ttagacttag ttcctaaaaa atatgatgga
    11581 cttgatggaa ctgagcttgc aatttcagag tcacaagagc gtatggcagt agccataaaa
    11641 aaagaaaata aagataaatt tatacaatta gcagtagaag aaaacttaga agctacgcat
    11701 gtagctacag taacagatac aggatattta agaatgttct ggaatggaaa agcaattgta
    11761 gatataaata gagagttttt agatacaaac ggagtaaaac aaactactga tgttcatgtt
    11821 actaaagtag atgaagaaaa tacattcttt agctctaatg aaatagtaaa agatgttaag
    11881 tgtgcaagta tgaaagacaa gtttacaaaa gtactatctg accttaatgt ttgttctcaa
    11941 aaaggattag tagagatgtt tgataataca attggtggta atacagtact tatgccattt
    12001 ggaggaaagt atcaagcgac tccaacacaa ggtatggttg ctaagatacc agtacttgga
    12061 ggagaaacta atacttctac aataatgact tatggataca atccaaaggt aggaaaatgg
    12121 agcccattcc atggtgcctt gtatgcagta gtagaatctg tgtgtaaatt ggttgctatt
    12181 ggaggaaatt atagtacaac aagacttaca ttccaagagt attttgaaaa acttggtaac
    12241 aatccagaaa aatggggtaa accattctca gcacttttag gagcatttta tgcacaaagt
    12301 aaatttgaga tacctgctat aggtggaaaa gacagtatgt ctggtacatt taaagatatt
    12361 gaagttccac caacattagt ttcatttgct gttgacacag tagatgcgaa gaaagtagta
    12421 tctccagaat ttaaaaaagc agactcaaaa gtagtaatgt tatgtgtaaa caaggcagaa
    12481 aatgatgttg tagattttga ggaattaaag agaaacttag ataaagtaag agaacttata
    12541 catggaaata aagttttatc aacatatgca ttaggatttg caggtgtagg tgaagcaata
    12601 agtaaaatgg cttttggtaa taaaatagga tttaagttta gtgaagaggc tgaaaaagca
    12661 tttacagatg ataaattatt tgaggctagt tatggaaata tagtacttga attagcaaat
    12721 gatgatttaa gtatgttaga aggatacaat tatgttgtgc ttggaagtac agttaaagaa
    12781 actagtatat ttatcaaagg tgaagaactt gcattagatg aattatataa agcacattgt
    12841 agtactttag agcctatatt cccaactaaa acagaagaag taaaatcaaa gatagaaact
    12901 ataagttata tatcacaagg agaagctaaa aaatcatcat taagtatagc tactccaaga
    12961 gtatttatac cagcattccc tggaacaaac tgtgaatatg actctgcaag agcatttgaa
    13021 agagcaggag ctaatgcaag tataagagta tttaagaatt taacttataa agacatagaa
    13081 gattctatag atacaatagt aaacgaaata aaatcttctc aaataataat gttaccaggt
    13141 ggatttagtg ctggtgatga gccagatggt tctggtaaat ttatagctac tgtatttaga
    13201 aatcctagag tgcaagaagc tataaatgaa tttttaactc aaaaagatgg actaatgtta
    13261 ggtatctgta atggattcca agtattaatt aaattagggt tagttccata tggagaaata
    13321 agagttccaa gtgaaagtgc tccaacactt acttacaata atattggtcg ccatcaagct
    13381 aagattgcta gaactagaat ttcatctaat aaatctcctt ggttagctca aactaatgtt
    13441 ggagatattc ataatatagc aatttctcat ggagaaggta agtttgttgc cagtgaagat
    13501 gttatgagag aattaattgc taatggacaa gtagcaactc aatatgttga ctttaataat
    13561 gaagctactt atgatattga gtttaatcca aatggttcat tctatgctgt tgaaggtata
    13621 actagtgctg atggtagagt gtttggtaaa atgggacatt ctgaaagaat tggtgaggaa
    13681 gtatataaga atattatagg tgaaaaagac caaaagatat ttgaatctgg agttaaatat
    13741 tttagataaa gctttgatgt ttaattgata gctagaatat ataatttaga tatttagatt
    13801 taaagagatg taataatatg atgataatca tttaatatta catctctttt ttatttatct
    13861 aagaaataat aagttcaacc taagttaaaa tacaagctgt gttataatta attattaagt
    13921 aaaattgaat atttattgga ggacaacata attaatgaaa atattaataa cagggtctaa
    13981 tggtcaatta ggaaaagaat tggttaacca attagaagca atcaatcaaa gtataaacca
    14041 accaaaatat gttatattag ctacaacaag aagtgatttg gacatatcca atcagactaa
    14101 tgtagataat tttattttgc ataataaacc tgatgtagtt gtaaactgtg cagcatatac
    14161 aaaggttgat gcatgtgaag ataatatcga aattgcatac aaaataaatg ctttaggagt
    14221 gagaaatcta gcaatagcat cagaaaaagt taatgctaag cttattcaca tatcaactga
    14281 ctatgtattc aatggttttt ctaaatatcc atatagggaa gacaataaaa ctgaacccaa
    14341 ttctgtatat ggaaaaagta aacttatggg agaaaaattt gttgaacaat tctcacataa
    14401 gtactttata ttgagaacag catggttata tggtaatggg aataattttg taaagaccat
    14461 gataaaactt tctttagaaa ataaagaggt taatgtggtt aatgaccagt ttggaactcc
    14521 aacaagtaca gtagacttag caaaaattat aataaaaatc atggaaacag aacattatgg
    14581 agtttatcat gctacatgtg aaggtgagtg tagttggtat gattttgcca aaaagatatt
    14641 taaactaaaa aatatagata taaaagtaaa tccaattaag agtaacgaat ttaaatgtaa
    14701 ggctccaaga ccactatatt ctgtattgga taattttatg ttgaagttaa ttggtttaaa
    14761 ttcttttaga aagtgggaag aatccattga agaatattta gaaagatatg atataaatga
    14821 aaagtaaatg tattattttg agaaaagaaa ataagatatt ataaaaaata taaatatttc
    14881 ttttatttat ccgataatca taatatgaca aaaacaaatt taaggatgga aaaagatgaa
    14941 aggaattatt ttagcaggag ggtctggtac aagactatat cctataacaa aatgtatatc
    15001 aaaacaaaca cttccaatat acgataaacc aatgatatat taccctatgt cggttcttat
    15061 gttggcaggg atacaagata tattaataat atctacacca agagatataa aagcatttga
    15121 agaattattt ggagatggaa gttcactagg gcttaacata acatatgcaa ttcaggagaa
    15181 agcaaatgga attgcagaag cgtttataat aggagaagaa tttataggaa atgacagagt
    15241 gtctcttgta ttaggggata atatatttta tggatatgga tttagtgaaa ggttggagaa
    15301 tgcagttaaa agaaaaggag caactatatt tgggtatcat gtaagtaatc caaattcgtt
    15361 tggagttgta gaatttgata aggattttaa tgtattgtca atagaagaaa aacctacagt
    15421 tcctaaatct aattatgcta taccaggatt atatttttat gataatgatg ttgtagagat
    15481 agcaaagcta gttgaaccgt cagatagagg tgaattagaa ataacctcta taaacagtga
    15541 atatctaaaa cgaggaaaac taaaagtgga acttctagga agaggtatgg cttggcttga
    15601 tactggtaca cataaaggtc tattggatgc ttctaattat gttgaagtgg ttcaaacaag
    15661 acagggactg tatatagcat gtttagaaga gattgcatat acaaaaggat atataagtag
    15721 agaacaatta atgaaactag ccaagccact gataaaaact gaatatggtc aatatctatt
    15781 aaaattggct agtgaagata tgtaatatgg tagataaaat ttattataaa ttttattaag
    15841 ggagtttatt ataatgggaa aaatcaatgt tataaagact aaaatagaag gattatatat
    15901 agtcgaacca aatgtatttt atgatgaaag aggatatttt atggaaagct ataataaaaa
    15961 ggattttata gagcagggtc ttgatatgtg ttttattcaa gataatgagt ctagctcaag
    16021 aaaaggcgtt ttaagaggaa tgcattttca ggttaaatat cctcaaggaa aattagtcag
    16081 agttacagag ggagaagttt tcgatgttgc agttgattta aggaaagatt cacaaacatt
    16141 tgggaaatgg gaaggtgtaa tattgtctga taagaataaa aaacaatttt atattccaga
    16201 agggtttgca catggatttt tagttttatc agaaaaagca gtatttagtt acaaatgtac
    16261 aaattattat tacccagaat ttgagaatgg ctttatatgg aatgacaagc aaatatctat
    16321 tgagtggcct ttaaaaggaa ttgaagaaat atttttatca gaaaaggaca aaaaacaaaa
    16381 aagttttaaa gatatttgta caagacgtta ataaaaattt attagaatta acatttagga
    16441 gaaaaaatat gaaaaaaata ctgataactg gaggagcagg atttattggg agtaatttta
    16501 tacattatat gcttaacaag tatgtagatt attttattgt taacctagac ttgttgactt
    16561 atgctggtaa tttagagaca ttaatagaaa tacaagacaa ttccaattat aaatttgtta
    16621 aaggagatat ttcaaatagg gagcaggtgt ataagctatt tgaagaagaa aggtttgata
    16681 tagttgttaa ctttgctgct gaatctcatg tagataggtc agttgagaat cctgatttat
    16741 ttattaaaac aaacattata ggaacacaag tacttttaga tgcatctata aaatatggag
    16801 ttaaaaggta tcatcaaata tctacagacg aagtgtatgg tgatttacca attgatagaa
    16861 aggatttatt ctttacagaa caatcttcta tcaatccaag ttctccatat tcagcttcta
    16921 aagcatcagc agatttattg gtaagttctt attatagaac atatggattg ttaacaacta
    16981 tatctaggtg ttccaacaat tatgggccat atcattttcc tgaaaaattg attcctctta
    17041 tgattttaaa tgctttggaa aacaaacaat tgccagtata tgggaatggg gaaaatgtaa
    17101 gagattggtt acatgtatat gaccattgta ctgctataga cttaataata cataaaggaa
    17161 atatagggga gatttacaat ataggaggtc ataatgaaag aagtaatcta gaagtagtaa
    17221 aaatgatatt aaatctttta ggtaaatctg aagagttaat atcctatgta aatgatagac
    17281 ctggtcatga tttaagatat gctattgatg caactaaaat agagaatgag ttaggttgga
    17341 aggcaaaata tgactttgat ttgggtataa aagaaactgt taagtggtat atagaaaatg
    17401 aatcttggtg gaaagctgtt ttatcagtat aactttaaaa tttagaaagg ggcgttttat
    17461 attgaatgag ataagttcac taattaatat attagcattg actcaattat ctggtagtaa
    17521 tactacaaat caatgtaatt gtacatataa taattcatct ggatttgata tgattatgat
    17581 gaccttgtta aaagcattat cacaaaataa tcaacatact tctaatagct acaatttatt
    17641 gtctaattct gagaatttat ttaatgaact tgataatgta gtaaataata ccactagtag
    17701 atttatagat gttgatacaa aagataaaaa tgtaaagttg agaattgaaa atgctgtaaa
    17761 tcaagcatca aaaaaatata acgtagattc aaatcttata aaagcaataa ttaaagttga
    17821 atcagatttt aatccaaata ccgtttcttc tgctggtgct aagggtctta tgcaacttat
    17881 gccagaaaac tgcagagact taggggttac agaccctttt aatatagaac agaatataga
    17941 tgctggaact agacatatta aggaatacat tgatatgttt ggagggagca tagaaatggg
    18001 attgatggca tataatggtg gtcctggaag aatgaaaagt agaggagtag aatcaatctc
    18061 tgacctgtat aaaatgccaa aagaaacaca aaattatatt cctaaggtta tgaaatatta
    18121 tagagggtag gctttattat gggaataaat atagataagc taaacataag tccaagtata
    18181 aatatggagg ttaaaaaact aaaagagagt aataaatttt ctgacaaatt aaactcttta
    18241 aaacaatctt cttcattgga agatataaaa cgaaatttgg aaagtgtaaa aaaggcagga
    18301 gaaaggcttg tacttactca aaactatggc gatattagta tatataaaaa tgcagtaaaa
    18361 gaatacctaa aatctgttgt agataatatg tattcacttg ataaaaatag tagcttttgg
    18421 gagcatcagt attataagaa tgtaaagatt gttgatagta aacttgagga tatgactagt
    18481 aagttaatta gtgaagaaaa agaaaacata gatattgttt ctactgttga catgatacaa
    18541 ggcttattga tagatatgta tagataaggt tatacaagga gaaaaatttt atgaatagca
    18601 aaaaagagga tatatatgaa ttgaattttg ggcaattaat agaagaaaaa tctggtgtta
    18661 tagaagaaaa tcaaaatgtt tttaatgcaa aagtaaaggt agctgtttct ataggaaata
    18721 caacagaaac aataaaaaat atattagatt taaaagaagg ctctataata aaattaagta
    18781 agaatgtgga tgaaaaatta gatatatatg caaacgatag attgtttgct tatggagaaa
    18841 gcattattac aaatgagaag ttatctgtaa gattatctaa aatagaagat tcagatgtaa
    18901 attagcattt taaatatatc cctttagtct attctgtatg agtttagcta aggggatatt
    18961 tttataaaaa atttaaaata tgtgtgaatt atataaatat ttttatttta tgagcgataa
    19021 ctaaattata agtatgtaat tttggaggtg aaactatgga tattaaaaat gtaagttcaa
    19081 atattgtagt gaataattat ttaaagaata catctggtat aaaaaaagtg gaagaggtta
    19141 aaaaaattaa taaaacagac aagatagagt tatctaaatt tgcaaaggaa atggcaagtg
    19201 tgtcaaatat gagtgatgag aatattagaa ataaaaaagt cgatgaaata aaaaatgcat
    19261 tagaaaatgg aacttacaaa ataaaaccaa aagaaattgc gaaaaaaatg atagaggata
    19321 tgcgaggata aagtcatgat tacagactta aaagttataa tatatgaaga aaaaaatata
    19381 attagtaaga tgatggaatt attaaaagaa caatttgatt atatagttga aaaagactta
    19441 gaaaatttaa atatgttaaa tcctaaacta gaagatatta gcagggagct tgcatccata
    19501 gaaataaaga gaagacaact ttttggtaat gatatttcaa tgagtgaagt tgttgaaaat
    19561 agtaatgatg gatatttaaa agaaatatat acagacttga aacgcattct tgctttagct
    19621 aaaaatcaac aagaatcaaa tgattctcta ataaagaaag aacttatttt tacgaaaaaa
    19681 atgattaatt tcataaaacc agctgataag caaactacaa cttataattc ttatggaaat
    19741 attagaaaat aaatattagg aggagaaata tggcaggatt attttccatt ttaaatacag
    19801 ctaattcagg aatgaatgcg caccaaaaag caatacagac aatttctcat aatataagta
    19861 atctagatac agacggttat tctaggcaaa ggaccgagtt tgctacaaat agtccaatgt
    19921 atatgcctag tttgagtaat tcaattgcaa gaggacaatt agggactgga gtacatgtaa
    19981 cggatgtaac aagagctaga aatagttttt atgattacca atttagggca gaagctcata
    20041 agtatggtaa gacagtttca aaatatgatt attacaatac tatagaaact atactaaacg
    20101 aaccatcaga ctatggaata tctgcaggta tagatgattt ttttaaagga tgggagtcat
    20161 tatcaaagga tataaatagt gacagtaaaa aaagtctagt ggtagaaaac gcatctaatt
    20221 tagcaacttt aataagtgaa agttataaga agcttgataa gttaaaagaa gatatagatt
    20281 ctaatataga cactgagata aaacaaataa atgacatgtt aaaaagtctt gaagatttaa
    20341 ataaatctat tgatattata tctggttctg gctctacacc aaatgatttg cttgatgaaa
    20401 gagatagaat tttagataac ttgagtttta aactagactt tgaaaatagt gatgttaaaa
    20461 atatgcttgc agatggtaaa ttagaactta atgaattaaa aaatccagat ggaacttgga
    20521 aaacaggtat atcagggacc ttacaaggct tgtttgaaat gcatggtaag attgatacat
    20581 ataaaagcga tttaaaagat gtatctgatg gattagctaa acagataaat gatgtttata
    20641 atacgtctgc aggaataaca gtaacagatt tttttataac ttcaaatgta gatggtgaag
    20701 atattataaa gataaatcca gctataaaga gtaacccaaa tgaattaaag cttactgctg
    20761 atgaagcttc aaaaattgct aagttaaagg atgaaaaaat agatattggg gtggctggtg
    20821 gaaaagtaag tacaataagt gaccactata aagcctttgc agaatcaatc ggtttagatt
    20881 ctcaaaaagt aaatcaagat gaagcaaatc aacgtaaaat tataaataat attgataact
    20941 caagaatgag tgtttcaggt gtatctttgg atgaagaaat gacagagctt ataaaaatac
    21001 aacgttcata tcaagctagt gcaaaagtta tgtcaacagc tgtgcaacta ctggatgttg
    21061 taataaatgg aataatttaa tagaaggggg aatttataat gagagtcagt acaggtatga
    21121 tgtctagcag ctatctaaac tcactacaag ataatctaca aagattggat aaggttaata
    21181 gacaagttaa tacaacaaaa gaaataaaca agctatcaga taatccatat aaagcaataa
    21241 agatattaaa ctcaaaaagt gaaataaaga ctatggagac ttatgttgag aattgtaaag
    21301 atacagggaa ttggttagaa actactgata ctgcattaga tcagcttgga aaattattat
    21361 cagacataaa gaaaactttg gtggcaagtg gtgatggtgc atactcagat gatgaattaa
    21421 agactcttag taatgaaact aatgaaaaaa tgaaagaaat tgcaaatgct ttaaatgcaa
    21481 cacatgaagg gaagtacata ttttcaggtt caaatacggg ggtaccacct gttgaatgta
    21541 tagataatgc tgatggttca gtgagtttaa agtttaattc tagtctaaat cttaataaat
    21601 taaatgactc tttgagtatg gatatagctc aaggaataag tgtagattat aacgttaaat
    21661 tgagtagcct tggatttgac ccaacaaaaa ctgacccatt tgaagagtta aataacatat
    21721 caaaaaaatt agcaaatcca gatgctgatg ctataaaaga gcttacaaca acttgtttgg
    21781 gcgatatgga acaactaata gaaaatacag taaatgttag gtctatatat gggacaaaag
    21841 caaatacagt agaggctatg aaggaaaaaa acgatgatag tttgatacag ttaaaggata
    21901 ttttgtcaca aaatgaagaa atagattatg gtgaaaaact tgttcaatta aaggcagcag
    21961 agttaactta tcaagcttca ctacaaacag gaggaaagtt atttaatgta tctatattag
    22021 attacatata ataaaataac gcagatatga tgaaggttac attaaaaaaa ggtatattag
    22081 gttttgaaaa tttaaaagaa tatgagcttt tagatataga agataatgat tatttaaaag
    22141 aatttaattc tactgaagaa gaaggtatag gatttgttgt tgtatctcca tttgagatta
    22201 tcaatgaata tgaaatagtt ttaaatcagg aaacaataga aaaactagaa gtgaaatctc
    22261 caaaggatgt tgtattattt aatataatta ctcttggtca gacatttgaa gaatcaactg
    22321 tgaacatgaa agctcctata gtgataaata taaaaactaa ttttggaatg caaataattt
    22381 tacaggatga taaatattca ataaggcatc cattattgag aggtgataat ggatgctagt
    22441 aatttcaaga aaaaaagatg aagcagtact tataggagaa agcatagaag taaaaattgt
    22501 aggagtagat ggaaacaatg taaaactagc tatatcagcg cctaacaata taagtatatt
    22561 aagaaaagaa atatacgaaa aagtaaagag tgagaatata aaagctacaa ataaaaatat
    22621 aaaaatttta aagtcattaa aataataagc ttggaggaag taatatatgt atggagaaaa
    22681 tccttataat tcttataaac aaaatgctgt ttttatggca tcaaaggaac aattgttact
    22741 tatgcttgtt gatggagcag taaaatatac caaaatagcc agagcagcta tcttagataa
    22801 aaatacacag aaagcgcata gagaacttgt aagagttcag gatatattta cagaactaat
    22861 ggttacatta gaccaaaatg caggacaatg ggcaaaggat atgtacaaag tttatgattt
    22921 tgtaagatat gaactttcaa gagccaatat tagaaaagac atccaggtaa tagataatgt
    22981 gttacctgta atagaacaaa ttaaagatac atggcatgaa gctgataaaa aaagtaaaga
    23041 agaaagaagt aggtacaaat aatggaatta aatttagctg aattagataa gttatcaaaa
    23101 gaggaattac tattgatgct tgtagatggc acagtaaaat ataccaatat atccaaagaa
    23161 gctcttttaa ataacgacta tttgaaagca cataatgaac ttgtaagagt tcaaaatata
    23221 tttacagaac taatgactac attagaccaa aatgcaggac aatgggcaaa ggatatgtat
    23281 aaagtttatg aatttataaa atctgaatta gcaatagctg atgaaaataa agatattaaa
    23341 ataattgatg atatattgcc aatagtaaaa caaattagag atacatggca tgaagtttac
    23401 aatcaactaa aaatataatt agatggaggg ataaaggtat gtcaagtata agtcctataa
    23461 gagttacagg tctttcagga aattttgata tggaaggcat aatcgaagct agtatgatta
    23521 gagacaagga aaaagttgat aaagcaaaac aagaacaaca aatcgttaaa tggaagcaag
    23581 aaatatatag aaatgttata caagaatcaa aagatcttta tgataaatat ctaagcataa
    23641 attctcctaa tagtatagta agtgaaaaag catactcttc tacaagaata accagttctg
    23701 atgaaagtat tatagtagca aaaggttcag ctggtgcaga aaaaataaat tatcaatttg
    23761 cagtttctca aatggctgaa ccagcaaaat ttactattaa attaaattca agtgaaccta
    23821 ttgttcgaca gttccctcca aatgccagtg gagcgagttc tttaactata ggagatgtaa
    23881 atataccaat atctgaacaa gatactacaa gtactattgt aagtaagata aactcccttt
    23941 gcgcagataa tgatataaag gcttcttata gtgagatgac aggtgaattg attatttcaa
    24001 gaaaacagac tggttcgtca tcagacatta atttaagagt aattggaaat gacaatttag
    24061 ctcagcaaat tgctaatgat aatggtatca catttataaa tgatgctggt ggaaacaaag
    24121 tggcaaatgt atatggaaag aatctagaag ctgatgtaac tgatgaacat ggaagagtaa
    24181 ctcatataag taaagaacaa aattcattta atatagataa tattgactat aatgtaaatt
    24241 caaaaggaac tgcaaagttg acttctgtca ctgatactga agaagctgtt aaaaatatgc
    24301 aagcatttgt ggatgattat aataaactga tggacaaggt ctatggttta gttactacta
    24361 aaaaaccaaa agattatccg cctcttacag atgcccaaaa agaagatatg acaactgaag
    24421 aaatagaaaa atgggaaaag aaagctaaag aaggtatact tagaaatgat gatgagttaa
    24481 gaggttttgt tgaagatatt caatctgcat tttttggaga tggaaaaaat attattgcat
    24541 taagaaaact aggtatcaat gaaagtgaaa attacaataa aaaaggtcaa atatcattta
    24601 atgcagatac tttttcaaag gctcttatag atgatagtga taaggtatac aaaacactag
    24661 caggttattc ttcgaattat gatgataagg gaatgtttga aaagctaaaa gatattgtat
    24721 atgaatattc tggaagttca acttctaaac ttcctaaaaa agcaggtata gaaaaaactg
    24781 cttctgctag tgaaaatgta tattcaaaac aaattgcaga gcaagaaaga aatataagca
    24841 ggttagttga aaaaatgaat gataaagaga aaagacttta tgctaaatat tcagccttag
    24901 aatctttgtt gaatcagtat tcttcccaaa tgaattattt ttcacaagca cagggtaatt
    24961 aattatggat aaattaaaag aaaaattaaa tttatataaa gatatatctt tacaaattat
    25021 aaatttgatt gaaaaggaag aatatataaa gataagtatt aagttaggtg aaagacaaga
    25081 gattataaat agtgtaagtg aaattgatag aaatgatttt atacagcttt ataatagaat
    25141 ggaattaatt gaaattgata gcaggataag agatatttta caaggacaat tattagaagt
    25201 aaaaaaagaa ttacatgagt ataagcttac taaacaagtg aacacaatgt attataactt
    25261 aaatagagaa aaagtaaata tttttaataa aaaagtataa agttatgtac catcttgtcc
    25321 gattatataa atgaagaatt aataaaaaag attaaaagta gaaatgacaa ggatgtcaac
    25381 tatactaagg agggtaaaat aatgagagtt aatacaaatg taagtgcttt gatagcaaat
    25441 aaccaaatgg gaagaaacgt aaatgcacaa agtaagtcta tggagaagct atcttctggt
    25501 gttagaatta agagagctgc tgacgatgct gctgggcttg ctatatctga gaaaatgaga
    25561 gctcaaataa aaggtttaga ccaagcagga agaaacgttc aagatggtat atctgttgta
    25621 caaacagcag aaggtgcatt agaagaaact ggtaacatac tacaaagaat gagaacttta
    25681 tcagtacaat cttcaaatga aactaatact gctgaagaaa gacaaaagat agctgatgag
    25741 ttactacaat taaaagatga agttgaaaga atatcaagct caattgagtt taatggtaag
    25801 aaattacttg atggaagttc tacagaaata agattacaag ttggagcaaa ctttggaaca
    25861 aatgttgcag gaacatctaa caacaacaat gaaataaaag ttgctctagt aaatacttca
    25921 agtataatga gtaaagcagg tataacaagt tctacaatag caagcttaaa tgcagatgga
    25981 acttctggaa caaatgcagc taaacaaatg gtatctagct tagatgtagc tcttaaagaa
    26041 ttaaatacat caagagcaaa attaggggca caacaaaata gactagaatc aactcaaaac
    26101 aacttaaata atactataga aaatgttaca gcagctgaat caagaataag agatacagat
    26161 gttgcttcag aaatggttaa cttatctaaa atgaatatat tagttcaagc atcacagtca
    26221 atgcttgctc aagctaatca acaaccacaa ggagttttac aattattagg ataaaataat
    26281 tttgtatatt ggatatagaa gaaagggtaa ggctttgctt tactcctttc tttttttaca
    26341 aatatacttt tgaaaggaaa atttatatgt tacttagtat agttatgatt gttaaaaatg
    26401 aagaaaaaat acttgaaaaa actttaaaat cattaaccac attaagaaat tctatagaaa
    26461 gtgaacttat aatagtcgat acaggttcta cagatgatac tataaaaata tctaagaagt
    26521 acacggaaaa agtgtatttt cataattgga ataatgattt ttctagtatg agaaatatat
    26581 caatatccta tgctaaaggt gaatggttac ttatattaga tgcagatgaa atactaattg
    26641 attcttcaac tattgtaaaa ttttttaaag atggtcttga taaaaaattt aattctgctt
    26701 caatacaatt aaaaaatctt tactcatatg ataaaaaatt atatggatat tgttcagtgc
    26761 ttagactttt taaaaacgtt gattttagat accatggtaa agtacatgaa caaccaatat
    26821 acaaaaaccc tatatttaat aatgttgctg attttgaaca ttatggatat ttgtttgaag
    26881 atgaagaaat aagattaaat aaggtgaaaa gaaatgaaaa actattattt caagagttaa
    26941 aagagaatga taattctcca tatacaaact atcaattagg gaaaaatttt attatattag
    27001 gaaaatatca ggaggcttta tactatcttg agaaatctta tgaattatat tctaaactag
    27061 aattggcacc tggttacttg ataacaagcc ttgtaaaaac atatttatat ttaggaaaac
    27121 ataagaagtg tgaaagattg tgcttaaagt atattaagaa agacatgaat aatattgata
    27181 tatattatta tattgcacaa gcacaagtag atttaggaaa gtatgaaaat agtatagatt
    27241 cgtataagag atatatttac ttgttagata attatgaaat aagtacacaa gcaaatagtt
    27301 tattttcaga cactgatact gtagggctta gagatgaggc tattattaca ctgataaaga
    27361 tttattataa gcttgaaaaa tatgagtttg taatttcaga gtataataac attgaagatg
    27421 tggaaaagaa aaagaatgtg tactttagct tatttatgtc actatataaa ttgaatagat
    27481 ttgaagatat aaaaaattat tataaagaag tttcaaattc taaaattgaa aaaaatagtt
    27541 tttatagaaa tatagaagaa gttataaata atattaaaga ggatgaaaaa gagtatgtat
    27601 ataaaatcct ttgtgacatt gaaggtaatt atggcatttt aaatcaaata aggataaata
    27661 aatgtgtatc tatagataaa tgtaaagaaa tactcaatga agaaaaggaa ataatttatg
    27721 catatattat aaaaatagct tttgaagaga agattgattt actagatatt ttttatgaaa
    27781 tggaatatgt atggatagag aaatatctaa aatatctttt agcattagaa agaagtttta
    27841 atttagtact atataagtac ataattagtc gtccaaatac atcagatatg gaaaagataa
    27901 gagtatataa agctatatct aaagtagttt tagaaagtat aagcttaagt gatgaaaaat
    27961 ataaggagat tttttatctt tatataatgt attcttataa gtacataaga tacatatata
    28021 gcaatttaag tgattttgaa ttaataaaat ttgtttgtaa tgatttagat aagttcacta
    28081 tagaatttaa gcatctactt gatattaaaa ataaagtaaa taatgctaat gaaaaattag
    28141 agtatataca tagtatgagg aaattactaa atgagtatcc tttttataat aaaataataa
    28201 aaatacttat aggtgaatta gaagaaaata taaaagaaag tgaagaattt aaggatttaa
    28261 aagatagttt tattattagt atagaaaata tgattgaaac tggcaaaata aatgatgcga
    28321 aattattggt agatgactat tcaaaaacat ttgtggatga tataaagatt ttaaatataa
    28381 agggaatttt atacatgttt gaaaataagc ttgataaagc tgattttatg ttaaaaaagg
    28441 cattatcatt agatgtagaa aatgaagata ctatatataa tatagaatac ttaaaaagtt
    28501 taagataagt caaaaatagg agaattgaag aatgaatata ccaatagtgg ttaatgaaaa
    28561 atctaaagac ataataaaag attggcaata tacatgtaca aatgaagata taaaatttac
    28621 aagcataata atattgacat ataatcaaat agaatatact aaattatgta ttgaaagtat
    28681 taggaaattt accccaaaga ataaatatga aataataata attgataata actcttctga
    28741 tggaacagtt gagtggttaa agggacaatc tgatataaaa tctgtgtata ataacaaaaa
    28801 cttaggattt ccaaaaggat gcaatcaagg tattgagtta gctactggtg aaaacatatt
    28861 gttgttaaat aatgatgtaa tagttactcc aaattggctc tataatttga ataaagcttt
    28921 atggtctcaa gataatatag gtgctgtagg acctattacg aatgcatgtt catattatca
    28981 gcaaattgat gttgattata agtcaattga ggacatgttt atatttgcac acaaaataaa
    29041 taaatctaat attaacctat ggaaatatag attgaagtta gttggatttt gcatgttaat
    29101 aaaaaaaact gttctggata atgtaggggt tttagatgaa ttattcacac ctggaaattt
    29161 tgaagatgat gatttatctt ttaggatgat agagtcagga tataagttat tattatgcca
    29221 ggatactttt atacaccatt ttggaagtgt gtcttttaaa gaaaatcgta ataaatatac
    29281 agatataatg tatattaata gaagaaaatt tgaaactaag tggggattta atccagaata
    29341 tagtggaatg ataagaacag atttgatttc taaaatagat gatagtttgg aggcaaatat
    29401 taatgtttta gaaataggat gcggtatagg ggctactctt ttagaaataa aaaatatata
    29461 taaaaatgca tcaatatatg gaatagaaat tagtgagtca gcaggtaaac ttgcaaagta
    29521 tagtgcagat gtacttatag caaatataga aaaagtaaaa ttggattata aaaaagattt
    29581 ttttgattat ataatattgg gagatgtact tgaacatttg attgacccat ggaagatagt
    29641 caatgaattg aaacaatatc taaagaagga tggttatctg gtatctagta taccaaatat
    29701 tatgcatgtt tctgttttaa gaaatcttgt gttaggtaat tttacatatg aagattcagg
    29761 aattttggac aagactcatt taaggttttt tacattaaat gagataacaa aaatgtttaa
    29821 tgatagtaat tacataatta atagtattac agctactagt atgccacttt cggaaattga
    29881 taatagcttc attgatacaa tatgtaagga aagctgtaaa gaaatgagac aacagtacga
    29941 atcttatcaa tatatagtaa aagcctctaa aatcctagat actaaaagat attctagtat
    30001 tagtatgatt aatttaaaat acgctttaat gagaattgat aatgaattag atgtagatag
    30061 taatttaagt tatatatttg ataattataa tataactgaa ccatgttttg ttgatgatat
    30121 tgaatatcta attgatacat ttgtgataaa taaagcaact ctattaaata gattaggagt
    30181 tgaggcattt aacagaggtc taggagattt tagtataaac atgtttatga cagtattaga
    30241 gatggataga gatgatattg actcaatata taatataaca agtgtattat gtgaattaga
    30301 agaatttgat actgcttata atttaattaa aaatagtagc gaaaaagtac agagtgaaag
    30361 tgatatacaa gagatacttt gttttattga ggggaagaaa tatgaataat gataagatat
    30421 gttttataac ctgtgtaaat gatgaaaggc tttatgaaga atgtcttgta tatataaata
    30481 gcctagacat tcctaaaaac tttagtatag aaacagtagc tttaagagga gccgattcta
    30541 ttgcctctgc ttataataaa gctatgagag aaacagattc taagtataag gtatacttac
    30601 atcaagatac ttttataatt aataaaaatt tcatacatga tattttagag atatttaaaa
    30661 aaaatacaga tgtcggaatg attggtgtag ctggagtaaa agatatgcct gtgaatggaa
    30721 tttggtggga ttctgataaa aaagcaggga aagtatatga tagtcataca ggtaaaatgg
    30781 atatttatga atttagtaac tttgaagaat gttttaatga agttatggct atagatggat
    30841 taattatgat tactcaatat gatgtatttt ggagagagga taagtttgat gggtggcatt
    30901 tttatgattt gtcacagagt atggagttta gacaaaataa atacaaagtt attgtaccaa
    30961 aacaaaataa gccttggtgt atgcatgatt ctggagtagt aaatataaat aatggatatg
    31021 atgaatatag gataaaattt gtaaaagagt acataaatct tgatttatat agagaaaatt
    31081 tttatgaatt tgataagaag aaagcaagta ccatacttaa gagtaataag catgtaatgg
    31141 attgtatgaa agaggagctt acagaacaat taagattaaa gaattacaag gatgttttag
    31201 gtattgtttc taaaattgct catttttctg tttttaatca tactggtttg tattcttgtg
    31261 aagaatgtga gagagcattg ctagaatgtg caaataattt accagaaata gaaaatacga
    31321 aattatttac aaaaaaagat ttaaaaagaa gaaaagtatt acatgtttta agtgaaggat
    31381 attcaacagg aggtcataca agactagtta aaaattggat tgaaattgac aaagactctg
    31441 ttcattcagt tgtaactaca tggcaatcag atacactgcc agactggtta ttaaaagcaa
    31501 taaatgatag tggtggcgaa gttatatctt tggatttaat ttcagatgat tatttaaaaa
    31561 gagctgtagc tttgaggaaa atagcttatg actgggctga tatagttatt ttatatgttc
    31621 atatgtggga tacaatacct gttgttgctt ttggagttga tgggaaaata cctattttac
    31681 atgttaatca ctatgatagt actttttggt tagggacaag tattgcagat attgtcattg
    31741 attttagcga agctagtcaa ttaattacat tagataaaag aggcatagat aaatctgaaa
    31801 tattaccaat gccattaaaa gaaaaagaag tattttcaaa atctgaagtt agaaaacgat
    31861 ataatttaga ggatgatgtg acaataatat tgactattgc tagtgaaaga aaatacagta
    31921 gtgtaaatga tattaattac tttaaaattg ctaaaacatt gattgaaaat acagataata
    31981 caattattat tgttgtagga gttgatacag agaatctcta ttggaaaact ttaagtaacg
    32041 ttacagataa tagagttata tgttttggaa caacagcaaa tatagacgat ttttacaaga
    32101 tttcggatat atatatggag tctttcatgg taggaagtca tacttcaaaa ctagatgcaa
    32161 ttttatatga tttaattcct gttaagttta aaaatgaagt ttcaccaatt tttacaaata
    32221 tgtggaattt tatagataag tttgattata agaatataaa agaggttata gatatgataa
    32281 aaaggtacaa tactagtgaa tttgacaggg ataggataaa agaaaaacaa tcggaaataa
    32341 aacattttgt tatagataaa cactgtattg aaataaaaaa ctatatagac aaaatatata
    32401 atgaagctaa tcatcataaa gttaagtatg atttatataa aaatgatgaa atcgaggact
    32461 atcagctttt tgtagcatta tatggctata tggtaaaaaa taaaaacatg tcataggtgg
    32521 taaaatgaac aatattcata tcaataaatt tgaaaaaagc tgtgaaagaa actgggatta
    32581 ttttgtagaa aataaatcta taaatggtac atttctgcaa actagaaatt tccttaatta
    32641 tcatgaggag ggaaaattca ctgaccactc attaataata tgtaaaggtg aagaaatact
    32701 agcagtcgta cctgcatgtg aaacttatga agatggtgaa agaatatttt tttctcattt
    32761 agggagtact tttggtggta ttataataag aagtggtttt aatgatatta ggcatgtaaa
    32821 tgccattaca aataagttag aaatatattt acgggaacaa aatttcgata aagtatattt
    32881 aaaaaataca tcaagaatat tttgtaaaga ggatacttca ttactagaat attttttatt
    32941 taagaataat tacatgtttt atgatgaatt aagtatgttt ttagaattaa gtaagtataa
    33001 agaagatata ttagccaatt ttaaatacag taaaagaaga gattttaaat attctttgaa
    33061 gaataatctt acctttaaaa gactgtatag tgatgaagat atagtttgtt tttttaattt
    33121 actttctaat tgtttagata gtaaaggagc taaagtaata cattcagtag aagaactttt
    33181 ggagtttaaa aataatagat taaaagatat tgtagaattt tatgcagtat atattgatga
    33241 cattatgatt tgtgggagca tggtatttaa ctttcaagaa agagtttatc atactcaata
    33301 tctagcatca aattcgacat ataataaata ttatccaatg atatttttat actataatct
    33361 aataaaagat gctttagaga gaaattttaa gtatctttct tttggaatta gcactctaaa
    33421 tagaggcaaa tacttagacg agggtttagc tacatttaaa gaaggttttg gttcaagtgc
    33481 aatttgtaac agaacatact ataaaaatat aaataagtga gaatcatgtt ttatagagtt
    33541 tttagaatat agtaaatatc tagtactcta acattacata aaaataatta gtttataaag
    33601 aaggtttgta tatgaaaaga ttaatgattt taggctcaat taagtatttt gagaatattg
    33661 ttatatcagc taaaaaagag ggaatatata cgattgtttg tgataataga attgatactc
    33721 ctgcgaaaaa gatttgtgat gaagcaatag atgttgatgt atttgatttt gataaaataa
    33781 aaagtatagc tatagataaa aagatagatg gaattttaac tggatttaca gattctttaa
    33841 tgaaaccata tgtttatatt gcaaatgaac taaatttacc atgtgtgatt tcttgcaatc
    33901 aacttgaatc tgtaactaac aaggctatta tgaaaaaata ttttaaaaat aatgcaatac
    33961 ctacaactga ttactgtatt attgaaagtc taaaagattt agaaaaaata caaggattaa
    34021 attttccact tgtaataaaa cctgttgatg gttatggttc aagaggtgta tattttgtat
    34081 caaatataga agaattaata gaaaaatttg aatattctaa ggaagcctca caagatggac
    34141 aagtaataat tgaggaattt tatgaaagtg aggaaataca aggactagct tgggttcata
    34201 atggagagtc acatgtattt tatattggag atagagagct tgtaaacata catacaggaa
    34261 gagctggaaa accagataga ttactttatc catcaaagta ttgttttcaa tatgaggagg
    34321 aaataaaatc tatatatcaa aatataacac aggcttttga tataaaaaat ggacctcttt
    34381 atgtacaaat gttagtggga gcatatggaa taaaggtatc tgaagtaatg ccaagattac
    34441 ctggaggttg tgactatatg gctatttctc aaataacaga tttagacata ggaagtttat
    34501 ttgcaaactt tagtgtgaat aatgaagtaa attttgatga aataaagaaa tataatatgc
    34561 atctatcaaa atgtgtatat gcattaccaa tatacataag acccgggaca ataaaaaaga
    34621 taaataatat agataaaata gaaaacttag attatgtcac tcaatttcta ttactagtaa
    34681 aagaaggaga tacaatagaa ttatatgaag atgagagaca ggattgtgga agagtttatg
    34741 gaatagcaaa taatatattt gatgcaaata taaaaaagaa atgtattcat gaaatgatag
    34801 aaatacttga tgaaaataat gaaaatatga tagaaaattt ttaaataagg gagtatgata
    34861 gatatgaagc taataactca tgaacaaatt atgtcattaa acatttctca gaaacaagga
    34921 tatgagtgga taaaagaatc tttcttaaca aaaaaagatt gtcaattacc accaaaaata
    34981 agcatggtag aagaaggaca tgtattttac aatgtaatgc catccctaaa tcataatcat
    35041 atgattggtg gaataaaaat ggttacaaga ttgccaggga gaaatccaag tttaaactca
    35101 gagatatttc tatacaatat ggaagatgct aaattaaagg ctattttgga tggtaattat
    35161 attacagctt ttaggactgc tgctgttgct gccttggcta tcgatacatt tgctaaaaaa
    35221 gattttaata ctattgctat tatgggttta ggtgtaactg cttctgctat aatgaatatg
    35281 ctttcatata ctcttaaaga taaaaatata aatataaagt tacttaaata taaagaccaa
    35341 gcagagttgt ttgtaaaaaa gtatgaaaaa aatgaacaat ttagttttga aatatgtgat
    35401 acacatgaag aacttattgt agattcagat gtagttattt ctactgtgac atatacagat
    35461 gaatatttat cagaatttaa atggtttaaa aaaggtgctt tattattacc tgtacatgtt
    35521 atgggatttg gaaatgttga ttatctattt gataaagtat atttagatga ttataatcat
    35581 ttgaaacatc tagaaaatat agatagatat aaatattctt gtgaatttta tgatgtttta
    35641 agtaaacaag ctaaaggaag agaaaatgat gatgagagaa taatatctta caatataggt
    35701 ttgtcaatac atgatatatt atttgcaaat aaaatttacg aattgtgtga aacactaagc
    35761 tgtgaagaaa tagaattaaa agaaccaaaa gaaaaatatt ggatatagtt tatttcattc
    35821 atatttacaa tagtttattg taaatatgaa tatgaagtaa tatattgtta gaaaattagc
    35881 atgaatagga tagataaatt tatggataaa tataatttaa ttaggttcgt atctattgga
    35941 aacgaatata taggaaattt agttgcatta gaatcaaata aagtaatacc ttttgatata
    36001 aagagagttt attacatata tgatgtacca aacaatgtaa agagaggatt tcatgcacac
    36061 aaaaacttgg aacaagtact agtttgccta aatggaagtg tgaagataaa atgttatgat
    36121 ggtgtaaaag agactatatt taatttacat aaaaatgatg ttggattata tataggaacg
    36181 ggagtgtggc atgagatgtt tgattttgat gaaaaaactg ttttaatttc tatagcatct
    36241 gaacattata atgaagaaga ttatattaga gactataaag aatttttaaa gtatttaaat
    36301 aatgtgtagt tattatacaa ttagtgatgt attttcttaa ttaaataaat ttaagatagc
    36361 cacatagaga atagatagat taggatgtga atttattgtt ggttaaattt aataatttat
    36421 taaaacagtt ccaaactcat aaagaagaat atgaacagaa gattaaggat gtaatggaaa
    36481 gtggatatta tatccttgga aatgaagtaa agcattttga agaagaattt gctaactatg
    36541 taggtagcaa atattgtgtt ggtttagcca gtggtttaga tgcactgata attgctttta
    36601 gagcattagg aataagcaaa ggtgatgaag taatcgtgca atcaaataca tatattgcta
    36661 gtgttatggg aatcagtata aatggagcaa caccagtatt tgtagaacca aatgaatatt
    36721 ataatataga ttcatctaag atagaagaaa agataaatag caaaacaaaa gctatattag
    36781 tagtgcattt atatggtcaa gcaagtaata tgaaagatat acaagaatta tgtgataagt
    36841 ataacttgta tttaattgaa gattgtgctc aatctcatgg agctaaatat gatggaaaaa
    36901 tgacaggaac atttggtgat ataggttgtt ttagttttta tccaagtaaa aatttgggtg
    36961 catttggaga tgcaggagct atagtaacta gtaatgagga aatagctgat aaagtgaaaa
    37021 ttcttagaaa ctatggctca gaaaagcgat attataataa atttgtcgga tataattcaa
    37081 gattggacga aattcaggca ggtttattaa gagtaaaact taaatatcta aatgaaattg
    37141 aaattgaaaa atcaaaaatc gtagatagat atcttaatga aattaataat cccaaagtca
    37201 tacttccaaa gatagaaaat aattgtactc atgtatggca tttatttgtt ttacaagttg
    37261 aaaaacgaga tacatttatg gaatacttaa agaaaagtgg tatagagtct ataatacatt
    37321 atccgatacc accacatcta tctgaagcat atgcttattt gggatataaa aaggggagtt
    37381 ttaatatagc tgagaactat tcagataaag ttgttagtat acctttattt attggtatgg
    37441 aagatgaaga agttagttat ataattaaaa ttataaat
//
LOCUS       DAMJYX010000027        36537 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMJYX010000027 DAMJYX010000000
VERSION     DAMJYX010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.027"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            90..764
                     /locus_tag="PC479_001512"
     CDS             90..764
                     /locus_tag="PC479_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804061.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            752..1912
                     /locus_tag="PC479_001513"
     CDS             752..1912
                     /locus_tag="PC479_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2804062.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2128..2655
                     /locus_tag="PC479_001514"
     CDS             2128..2655
                     /locus_tag="PC479_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2804063.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2941..3609
                     /locus_tag="PC479_001515"
     CDS             2941..3609
                     /locus_tag="PC479_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804064.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3612..6080
                     /locus_tag="PC479_001516"
     CDS             3612..6080
                     /locus_tag="PC479_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804065.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6320..7744)
                     /locus_tag="PC479_001517"
     CDS             complement(6320..7744)
                     /locus_tag="PC479_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804066.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7763..8455)
                     /locus_tag="PC479_001518"
     CDS             complement(7763..8455)
                     /locus_tag="PC479_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804067.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8507..9286)
                     /locus_tag="PC479_001519"
     CDS             complement(8507..9286)
                     /locus_tag="PC479_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2804068.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(9283..10053)
                     /locus_tag="PC479_001520"
     CDS             complement(9283..10053)
                     /locus_tag="PC479_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2804069.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10050..10754)
                     /locus_tag="PC479_001521"
     CDS             complement(10050..10754)
                     /locus_tag="PC479_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2804070.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11009..11521)
                     /locus_tag="PC479_001522"
     CDS             complement(11009..11521)
                     /locus_tag="PC479_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804071.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            11847..12104
                     /locus_tag="PC479_001523"
     CDS             11847..12104
                     /locus_tag="PC479_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804072.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12247..12873
                     /locus_tag="PC479_001524"
     CDS             12247..12873
                     /locus_tag="PC479_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2804073.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13120..13899)
                     /locus_tag="PC479_001525"
     CDS             complement(13120..13899)
                     /locus_tag="PC479_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2804074.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(13967..14428)
                     /locus_tag="PC479_001526"
     CDS             complement(13967..14428)
                     /locus_tag="PC479_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804075.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14404..15081)
                     /locus_tag="PC479_001527"
     CDS             complement(14404..15081)
                     /locus_tag="PC479_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2804076.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15314..15700
                     /locus_tag="PC479_001528"
     CDS             15314..15700
                     /locus_tag="PC479_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2804077.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15703..17502
                     /locus_tag="PC479_001529"
     CDS             15703..17502
                     /locus_tag="PC479_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2804078.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17857..19731)
                     /locus_tag="PC479_001530"
     CDS             complement(17857..19731)
                     /locus_tag="PC479_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2804079.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19736..21400)
                     /locus_tag="PC479_001531"
     CDS             complement(19736..21400)
                     /locus_tag="PC479_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2804080.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(21414..22235)
                     /locus_tag="PC479_001532"
     CDS             complement(21414..22235)
                     /locus_tag="PC479_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2804081.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22334..23098)
                     /locus_tag="PC479_001533"
     CDS             complement(22334..23098)
                     /locus_tag="PC479_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2804082.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23095..23844)
                     /locus_tag="PC479_001534"
     CDS             complement(23095..23844)
                     /locus_tag="PC479_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2804083.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24348..24953)
                     /locus_tag="PC479_001535"
     CDS             complement(24348..24953)
                     /locus_tag="PC479_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2804084.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25132..25770
                     /locus_tag="PC479_001536"
     CDS             25132..25770
                     /locus_tag="PC479_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2804085.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            25902..26564
                     /locus_tag="PC479_001537"
     CDS             25902..26564
                     /locus_tag="PC479_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804086.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            26564..27745
                     /locus_tag="PC479_001538"
     CDS             26564..27745
                     /locus_tag="PC479_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804087.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(27926..30586)
                     /locus_tag="PC479_001539"
     CDS             complement(27926..30586)
                     /locus_tag="PC479_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804088.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(30583..31266)
                     /locus_tag="PC479_001540"
     CDS             complement(30583..31266)
                     /locus_tag="PC479_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804089.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(31610..32563)
                     /gene="blaCDD"
                     /locus_tag="PC479_001541"
     CDS             complement(31610..32563)
                     /gene="blaCDD"
                     /locus_tag="PC479_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2804090.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(33104..35218)
                     /locus_tag="PC479_001542"
     CDS             complement(33104..35218)
                     /locus_tag="PC479_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2804091.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(35476..36336)
                     /locus_tag="PC479_001543"
     CDS             complement(35476..36336)
                     /locus_tag="PC479_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804092.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
BASE COUNT        12868 a         5843 c         4473 g        13353 t
ORIGIN      
        1 gaaatataac taagaatttg ataatttatt gagattttta tactaaaata tacatactat
       61 acaaacttaa tttagaaggg agcattctta tgaacaataa aatactcgtc gtagatgatg
      121 aggaaggtat aataacactc ttaaaagatt actttgaaat gaatggttat gaggtttaca
      181 ctgctcaaag tggaaaagac gcattaaaaa aaatatctaa aaatccagat atcattctac
      241 ttgatataaa catgcctgaa atagatggaa ttgaagtttg taatagaatt agagattatg
      301 tatcatgccc tattctattt cttacagcta gaattgaaaa tactgacaag ataaatggtt
      361 ttcgtgctgg tgctgatgat tatattgtaa aaccttttga tttagacgaa cttggagcta
      421 gagtatctgc ccatttaaga cgtgaacaaa ggaagtcaaa tcaaactaaa attaaatttt
      481 ataatgattt aacaatagat tacgaaaaaa ggttaatttg tatatataat aatcagattt
      541 cactttcaaa aaaggaattt gatatagtag agcttctctc tatgaatcct ggacaagtat
      601 ttgataaaga aaaaatatat gatagagtct ggggatacaa tagcaatgga aatagtgatg
      661 ttataatgga acacattaga aaaatacgaa tcaaactatc aaaacatact cttgaaaatt
      721 atatagaaac agtctgggga gttggttata aatggataaa ataaataata tgtcattaaa
      781 aaaagctttc tttgttctta ctttatcttc actaattata gcatcagtgc ttactacttt
      841 cgcatacatt ttacttaata atctatataa ttctatacaa gataagtatt taaaaacacc
      901 tataaataca ggtattataa ccatagttca aaacaatgtg aaccaattta gtgattatga
      961 taaaacatta cttaatataa tcaacatact tcagcttgta cttccactta tcttttttat
     1021 aggactttta ttattagctg atataatttt ttacaaggta aaattaaaaa cacccataga
     1081 aatactcaat aaaggtgcaa tggaaatatc caacaacaat ttagactttt gcttagaata
     1141 taataataat gatgaattag gtaatctatg taatgctttt gaaaaaatga ggtctgaact
     1201 aaataaaaac aacataaaga tgtggactat gattaatgat agaaaacaac ttaatgctgc
     1261 tttttctcat gatttaagaa atcccctaac agttctaaaa ggttactcaa actatttaac
     1321 aaaatacata ccaacaggta agcttagtga tgagaagatt ttatcaacca cacaacttat
     1381 gtctgagcat attaacagaa ttgaatacta tgtagaaaat atgagtaatg ctcaacgatt
     1441 agaagattta gttgtttcaa aatctatgtc gaatataaac aagtttatag aaaacttaga
     1501 tgaaaatata tctatcattg ctaaacaaga aggaaaatct tttacactta aaagtcaaat
     1561 aaataatata aatctatttt ttgatgaaaa tatcattctt agagtcgtcg aaaatataat
     1621 atcgaatgct tttagatatg ctagaaataa tgtatccata cttatatatc ttgaacaaga
     1681 actccttacc tttgtaattg aagatgatgg tataggattt agtaaggaat ctctaaaatt
     1741 agctttaaga cctttctata gagataagac tttaaatgac agcaatgcac actttggtat
     1801 gggtctttat atctcaaaaa tattatgcga aaaacatggt ggctctattt ctattgaaaa
     1861 taattctact aacagtgcta aaattactgc taaattctca acaagaaatt aaaatgattt
     1921 tgtaagcttt cactgttctt atataaaatt tgttagttta gaatttataa aatgtatttc
     1981 aatttacaac caatatatat actattttgt agaagttgta agagttgctt caattataat
     2041 atttggcaaa aggcatttga aataataata gaaatatata tttaaatatg tagaatagta
     2101 tgaaattaga attttggagg tgattatatt aaaagttttg ctaataatat tacattctct
     2161 cgtattgtgt ttgcattgct aatgcttagt tctacattct cttcactgcc tttctggata
     2221 tgttattttt ggtgtggaat tagtgatata cttgatggtc ttattgcccg aaagttgaga
     2281 caacaaagtg gaataggtgc aaagttagat agtattgctg atttcatttt tgcggttaca
     2341 attttggtta ctgtcggaaa aaatattgct cttcccatgt atctatggtg gtgtattgtt
     2401 gcaatagcat tgttacggtt gattagttac agtattggtt tttttaagta tcgcacattt
     2461 tcatcgcttc atactttgat gaataaaatc actggaattt ctatctttgc ttttccatta
     2521 ctctatattt gctttggtct tgatgttgca ggaattatga tatgtatcgt ggcttttctt
     2581 tcttcttttg aagaaatagc aattatgata aaatcaaaag aattaaatcg tgatataaaa
     2641 agtatattta tgtgacaact tagaatttgt gacaaaaata atttatataa taatgaggaa
     2701 gtaaaacata aaaaattatc cacaagttta tgtgtaaaat agttttaaag caatagagtt
     2761 gtattgtaaa gcctttagtg cataaattac attttaaatt aaaaatgata ctgaaactgc
     2821 ttatcagcac tgcgaattat ccgcaaatag tgaagacttt ttagatttaa gctaaaagtt
     2881 gggaaaaaat tgagttttaa aacttacact cttcttatat agaaaaggag agtgtttttt
     2941 atgattataa aagcaaaaca attatcaaaa atatatggtt ctaataacaa taaagttata
     3001 gcacttaata atgtaaattt agaaataaac tcaggtgaat ttgtttctgt aattggacct
     3061 tctggaagtg gaaaaagcac acttttgcat attttaagtg gattagataa tcctacctct
     3121 ggacaagtgt tacttgatga taaagatata tataaacaca ctgaaaaaga actatctgca
     3181 cttagaagaa aatcctttgg attcgtcttt caacaattta atcttttacc tgttcttaca
     3241 gcatctgaaa atatttcaat gcctgtactt ctagataaaa aacaacctga taaagactat
     3301 ttaaatgaaa tttcatcatt acttggaatt gcagatagac tcaatcatct tccccatgaa
     3361 ctttcaggcg gtcagcaaca aagagttgca attgctagag cacttattgc aaaaccagat
     3421 ataatatttg ctgatgagcc tacaggaaac ttggacagta aaagtggcag tgaagttatg
     3481 aatcttctta taaaaacttc aaaacagttt ggaaagactt tagtggtaat tactcatgat
     3541 gatagaattg caaaacttgc tgatagaaaa atttcaatta ttgatggtgt tcttatggag
     3601 gtgaaataga aatgaagaat tacctttcac tttcaataaa agaattaaaa agccagaaat
     3661 ttatgactac attcatcatt atagcaatag tactatcaac tattatgaca actgtggttg
     3721 gacagtcaat tggtatccta caaaatttaa gaattgaaca agcccgttct tttaatggtg
     3781 atagacatgt atcctttcac caactcacta aaaatcaagt tgatgacata aagaaagatg
     3841 atagagttta tcaagctggt acttcaataa ctattggcag tagtaaaata aaggattctg
     3901 gtatctctgt acttgtaaaa gagtatgata aaacaggact ttctaactac ccaaaattaa
     3961 tgaaattaaa atctggacat ttgcctaaag ataaaaacga aatagcctta gatgaaaaca
     4021 cactaaagct tatgggtata aaaccaagac taggagtgac tatacctatg aatttagata
     4081 tttcactttt aaatgatacc ataccaccat ataattacac agctaacttt aaattgagtg
     4141 gcattttaga ggatgattat acaggatatg taagtggtat agtaaatggt attgtaggta
     4201 aaggtacatc agaaaatcta ttacctaaga gatatatttt atcctctttg gattttaaaa
     4261 taaaacagca agaaaagttt caagaaatag taaatcaatt agctaaaaaa ataaatcttt
     4321 ctcacaattc aattcaatat aattggattt acttaaatgc tcttaagata cagtttgaaa
     4381 aagatgaaaa tagttctaat agtgatggta tatctatgat tatattggta tctttatttg
     4441 ttgccttatt ggttttactg gcttctggac ttgtaattta taatatttta aaaatctctg
     4501 ttacaaagaa aattaaagag tatggatgtt taagagcaat aggagctgaa ccaaaccaga
     4561 tatataagat agttatttta caaatcttaa ttttatgtac tgttgccata cctattggag
     4621 ctgtaattgg aattatatct tctaaaggca taactggaat ggtcactaat atcttaaatc
     4681 cagatatact tctagcaaat gataataaag aaattactga gcttatacat aaaaatacaa
     4741 cagcatatat attcccatta gtattaagca ctaatgtatc tttgattttt tcatttattt
     4801 cagcattacc atcagcaata tatgcttcac atgtctctcc caaaattgct atggttggta
     4861 gtactaccaa aatcaaaaga aaaattaaaa gagaaaaaac aataaaaaac tttgaaagac
     4921 atctagcatg gctaaactta aaaagaaata aaggaagaac tataataact atactatcat
     4981 tatttatgag tataacagta ttcgttgcac tgagtgagtt ctctaatgta ttagatgtat
     5041 ctaggtcagt aagtaattta aaagaaggag atttttcctt aacaaatgaa atatctggtt
     5101 ttgataaatc aacactagat aaaatagcta aaatgaaaaa cgtcaatcgc acttccttta
     5161 taaagtactc tgaatacaaa cagggtgaaa tagatacaga tataaatttt gaaaatagtg
     5221 gtgagatgtt aaaaatcatt ggaattgatg agcaaacttt aaaagactta atgccttcta
     5281 ttacagattc tatactagaa gactttaaaa atggttctat ctgttttata aaaaatccga
     5341 ttgctatttc tacaccaggt gtaaaaacta gatatacaaa tttaaaacct aaagatagta
     5401 tcacaatcaa caaaaaacaa ctagatatat atagtacagt agataaaatg ttctttttac
     5461 aaggcaatgg ctggataaat ggtgttgatg ttattgtata tgattctgtt tataacactt
     5521 tgacaaataa aaacaagttc aaccagataa atatttatgc taaagataaa tcaaaattag
     5581 aacaaataag attgtctatt gaacagattt gtgaaaataa tcctggctct cattggatat
     5641 catatattga atcagataaa caacttaaag agagttttaa acaaatagaa tttctagcat
     5701 gggcagttat attatttgtg ggattgattg gtactttaaa catcattaac acaactcata
     5761 caaatataaa tactaggact aatgagattg gagttaaaag agctattggt atgagtaata
     5821 ggagcttata taaaatgttt ttatgggaag gtgtttatta tggaattttt gctgctatat
     5881 ttggttcaat tgctgggtat gccagtgcta ttatcataaa catggcaact atagaaaagc
     5941 ttgatttcac taatatacca attacttcaa ttttacaagc tactattatt tctgtacttg
     6001 catgtattat agctactctt attccgttaa gaaaagttaa aaagatgaat ataattgact
     6061 gtatagatat caatttataa ctattttaca gataataata taaattaaat tatcaatgac
     6121 tgtctctatt ttctttcaaa tctaactaat aaatcttttt aactataata taagctttaa
     6181 aaaaggcctg tctcaaaata gagataagcc ttttttcatt gtcgaaataa tgaaaatata
     6241 tttaacttca caaaaaaaga gtgcctcacg aattaaaaat ttcgctttga gacactctcc
     6301 tttaatttaa aatatatagt taaatgctat gccaaaattt cacacatgct ccacctgatt
     6361 ttgaattttc tattttaaca gaacctccaa gctgttccac taattgtttg acaatgtaaa
     6421 gcccaagacc agaatgtcca ccttttgtct gcctagcttc atctcctcga taaaatttat
     6481 caagagcctt ttttaaatct ttagagctaa accctctacc agaatcacaa atctcataag
     6541 aaatacaatt tttttcatcc tttacagtaa tactaatatt accacctgat ggagtgtatt
     6601 gcagactgtt ggatatgata ttatcaaaaa ttcgtgtcaa cctatcaacg tcgatttgaa
     6661 ttggggattg tatattcccc tgcattttca atataagttc aacttccttt tgatgagcct
     6721 gtaattcata gtcttgcacc ttttgcctca aaaattcagg taaatttata ggaacaagat
     6781 ttaattgtat atttgacttt tctaagtcag acgtatattg catcttttgg acaagagcag
     6841 cacttttttc tgtattttct cttatcactg taagatacct atgaagtttt tcattgtcat
     6901 ctgtattatt accaataagt gcatctgtat atcccaaaat aatagaaaga ggagatttta
     6961 aatcatgtgc tagagcttcc accatttcaa cacgttcctg ctccatcttc cactgtgcag
     7021 ataaagagcc ttttaattcg tctttcattt cagaaaaggc actacaaagt ttaccgagtt
     7081 cattatctga ataatagtcg atttcaaaat caaggtcctt ttcttttatc ttcttggacg
     7141 catcaatcaa tagatgtaat ggttgattga tattctttac aaagcgctta gaaagccatc
     7201 tagtaaaacc cacgatatat agaaatggag aaaacaaagc cattataata actgcaaata
     7261 caaatctccc tctaatattt gcaaaggtga ttttcacttg gtagaataga ataacagcac
     7321 cctcaatctt tccattatcc cctttgattg gaacagtatg gatataacca cctttacgaa
     7381 cagttttatt gataaaatta ttaaataatt cttcttttgt tttaaatagt tttttggggt
     7441 tagtaccata taaaatattg ccattattat ccacaacttg atatagcata tcatccccac
     7501 gaatcgttct ttttaaacct tcttcattcg attgtgataa caaagctatg tttttttcat
     7561 tgatgtattt ttcaattcta ggaacttgtc gctcataata gtttggtgga tagatatcct
     7621 tatttaagga atgaatatac aaatagagag caaatacata agtaatcact gtggcaacta
     7681 tactggctat tataataaaa ataaaagtat gtctaaattg ggtaataagg gattgatttt
     7741 tcatttaata acctctctct ctttacattt tattccactt atacccaata ccccaaactg
     7801 tggaaatata ttctttttct ggtgtcacag cagaaaactt agctcgtatt ttttttactc
     7861 gttcaacaac agttgaggaa tcaccttcag aatcacaccc ccatactttt tcatagattt
     7921 gttctcttga gaaaacttgt cctgcatgga gtgctagtaa ttcaacaata tcatactcta
     7981 tttttgttaa atcaatattc ttaccatcaa ttttaattgc acgttccttc atatataagc
     8041 ataacttccc aaaatacaaa gtagatctct tgttctcttc attgatatac tgtgaacgct
     8101 tttccctacg cagattagct tcaatccttg ccataagttc acgcagtcca aatggctttg
     8161 ttacataatc atcccctcca aaggtcaacc ctttgatttt gtcggtttct gattgctttg
     8221 cacttaaaaa tataattggg cataaaacat catctctaat tgcctgacat acctcaaatc
     8281 cattcatttt aggcatcata atatccagta taataaggtc aggctcctgt tttgataact
     8341 ctatacctgc ctgaccatca taagctatca acacttcatg tccttttgcc ttcaattcat
     8401 cctcaagtag cattactagg tccatttcat catcaataat caatatttta ctcaatataa
     8461 caccaccttt ctcttatggg aaggtcagta ttaaaacaat attcgtctac tcataacttt
     8521 tccttccctc ccacatttta aaccaaataa cgcctcctat caagaatata atcaaaccta
     8581 ttgctactac tataagctct atactagctg tttgcatcac tttagagtaa aatgcctcag
     8641 ttgaaatagt tccagcttct ttaataaaat aaattcctgg aagcatacct agttttactg
     8701 gccatgtcca tggaacaaat gcccatactt tttcaccaac gctagttaat ccaagtattg
     8761 ctgccatcaa aattccacca atactaatac caatagatgc acccataccc catgcaaaac
     8821 tcacccaaag atgaatagct aatagtggaa gtgtcccaat caccacaagt cctgctgctg
     8881 acaggaataa ccatattgtt gctccagatg gcactgcaat actcattcct atgcaaagta
     8941 tcaacgtagc aataaatgtg catatagtta ggcaaaacag aagaaaaaga aattttccca
     9001 gatataatct gacacgagaa atacctacac ataaaaaacc attaaaattt ccagcaagct
     9061 cttcctcctg cactataaat cctgctaaaa tacctactcc aataggaatg atgaatacag
     9121 tccatactgt aaaaaatcct tcaaaagcaa attcttgtgt acttccagat ttagttgtga
     9181 gataaaaaac cacacataat gaaaaaataa taggcataca aagagtaagc cactggacag
     9241 ctgtcctctt tgctcgaagc cattctgatg ataatagttt tatcatttgt catccctcct
     9301 attgaaccaa gctgcagtaa gtgctgttaa tactataaac acaaccaaag aaacaacaat
     9361 cccaatagga atcactgaag tattcaaaag tgggtctcca gcttctaata tagtaccgtt
     9421 aggatgtatg ttgatgattg ggcataccat tctaactgcc caactccaag gtactgcaat
     9481 ccaaaatggt tttgtcgcag caagaacacc aaatatcata cctaaagctc caactcccat
     9541 acttaggaac atacctttcc atgtagctag ccatagctga agtggaagta tagcaagaga
     9601 aacaacccag catacaatac ttgcccctat aatctgtcca aaaggaactg gaccagcttt
     9661 caatattaaa ccagttataa ttgttacgac tattaaaact agcgttgaaa taaaactgta
     9721 gactgccata gctataactt tattaatcca aagtgtcata ggagacacat tgtgtgtacg
     9781 taaagcacga taatttccag cttttttttc ctggaaagct accaaagtag cgaacaatgc
     9841 aaatccaata ggcagaaata aaaatgacca ccagttaaaa attaatgcta aaagattttg
     9901 ccacgcccaa gaagttgttt gagataatgg attttgcatg agtgttgatt gcattataaa
     9961 tgcatatgct gcaaaagaca ctgggaaaaa aacaataagt ttacccataa atgttctttt
    10021 atactttaaa agttctgatt gtaaagcatt catctgtttt gtcctccttt aagaataaca
    10081 tctgtaaaaa attcttctag attttcatta gggtcaggca ttccttgaaa taatagtcgt
    10141 cctccattta taattcctat attatctaca acttgagcaa cctctgatag aatatggcta
    10201 gaaagaatta cagttatacc ttttttggga aaagaagcaa tcaattcacg caattcttga
    10261 atgccaaagg ggtcaagacc attggttggt tcatctagta ttaacaactc tgggtcactc
    10321 aaaagtgcaa tagctattcc taacctttgt ttcataccca tagaaaattg tgaagctctc
    10381 tttttacctg tatttttcaa atttactatt tccaacactt cataaatttt atctttaggt
    10441 attcccaata tttttgtatg tactaacaaa ttttcctctg ctgtaaggtt tccatacaat
    10501 gcaggggatt ctattaaaga acctattttt gcaagagatt ctcgtttcca aggctttcta
    10561 tcaaaaataa tacgaccatt tgttgggcga agaagaccaa caagcatttt aagtgttgtg
    10621 gactttcctg ctccatttgg tcctaaaagt ccatatatac tatttcgttc aatttgtaga
    10681 gaaacattgt taactgccat ttgttctcca tatctttttg ataagttttt tgtttcaaga
    10741 attaaatctt tcatattaat ttctcccttc ttgtaatacc ttaaatctgt tactaagaga
    10801 atactaacat gaaaaaataa caattctata acacctacta attttaataa aatttaaaat
    10861 aaaaaataga gttaccaatt ttattttggc aactctattt aagtttatgt tttacagtta
    10921 tattagattt aaacttagtt tcattatcac actaacttca aatttactct ataatcttta
    10981 aatcaaacac taatttatta atcaaacact aatctattaa tttatatttc tttagacatc
    11041 taatatagtt tgctactaaa taataattat ttatatcata tttatctaat cctttaaaag
    11101 aagttatttc atcattaagc tcatcactac cttctacaaa aacacaacct gtttcaaatt
    11161 ccatacctac aataatctta cagtcactat aaatttcctc ttgagtatcg tacaattttt
    11221 ttgcatttcc ttcatttgga attgtaaata caataggttt taattcttta tcactggaaa
    11281 tcttttgtga aaaaggcttg tttaacttaa tattattgat aactacatgt ttaaattcct
    11341 ctgaagtttc ttctactatt acatattttc gctttaaaaa ctctattatt tctaaaacag
    11401 acttagaatt tacttttaaa atacttttat atttttcata taaagatttc cactgttcaa
    11461 ttttttcctg tggtatttct tggatatgtg tacttatttt ttcttgagat atttttttca
    11521 attaaactct cctttcaata cttaaatatc cttatgaatc tcacatgact aaagttacaa
    11581 ggtttctact taatagaatt ttgcatactt aaatatgtct tattaattct ctacaggata
    11641 tctccgtagt tcctacgatt cttacataag ttacttattt taaattgtta ttagttatat
    11701 tctaaaaatt atttatttta tcactaatat aatactctta aaaactttac aaatcaattt
    11761 aaatttgcta ccttttattt ataacaactt atagtacatt tctcctatgc ttttcaatat
    11821 attactttta aaaggaggtt ttcattatga ttttaaatag agctgagctt ttccgaggat
    11881 attctttaaa tagatttaac gagatatgca atatattatc agtaaataaa attaaatata
    11941 agtataaagt gaaaaataat gcttactcta aaaaaccttt ttttaatgaa ataacacttg
    12001 gcaatttagg ccaaaaggaa gatttctctt atgagtactt aatttatgtt cataaagacg
    12061 attatgaaca ggcagagtct ctaataaatt ctaagttgcg atagattatc tataaaacat
    12121 gacattctgt tgtcatgttt tatatgttat tatactttca aatatgctac tatattttca
    12181 aaattgtgtg ctttaaaact agcttttttt agtaatttaa agcttaaaac taaggagact
    12241 cgttatatga aaataaatat aaaatcaaag ttaagtgaat ttattaaaca gaacaatctt
    12301 tttgatgaca gcagaataat atcctatctt cctaatataa caatagaaac agacaaaata
    12361 aaaactataa tgattaatga ggttgtccca actaacacca atgacgattt ttatagtgta
    12421 gataaagatg ctgattattt aaaaactact attcctttat ttgacagtgc aggaataaaa
    12481 gtatctaaca taaatgatat cctagatatg ggtatctata taactaatgc agtaaaactt
    12541 cctaaatcag aatatacaat aactcgtgat acaattaaat tacatatgcc tattttagaa
    12601 gaagaaatta aattatttaa aaacctagag gtagtaatgt taatgggaga tgttgcaaaa
    12661 aaatctttta atatgattac taaaaagcat ataaaaaaga atgtcatccc ttccatttca
    12721 acatataaac ttcgtaataa tgaactttat tatgataatt taagagtatt tccatcatat
    12781 attatgactg gtggaaacat actgattgaa aaatcaaaat ttgaaatggc atcagaagat
    12841 ataaaaaaaa tgttcgagat tataaacggt tgatattaca aaataataaa aactaatata
    12901 aaataaaaag tgcctcaaaa acttctattt atgaggcact tttattttat attagtgtaa
    12961 ctatatacta ttaacataat ttattattta tattgttctt tttaatctct attccattta
    13021 gtctaggtaa cattactctt tacaagatta ctttatattt cttttatatc tctaacgttt
    13081 ttatttaata aaacaattat taatacaagt caaatatatc tatttatcta aaaatatatc
    13141 ttttacagta atatctggat ggtgatactt cttcctattc gttaaaaaag atttttttac
    13201 attaatagcc tcttttggac accagtgaaa acaagctagg catctttcac actcattacc
    13261 ccactttggc tttttatcaa ctatacttat attctctaca ggacaaaccc ttttacatgt
    13321 cccacatcca ttacatttat tttctgcaaa aaatttagta gttgcaattt gcatattctt
    13381 acctactaaa cgaagtacaa cactatctat atgtgacttt aaattgtttt ttccttctat
    13441 atttccaatc tccaagttat tgatacattt tgcaatctca gctaccctat tagtctgatt
    13501 tttcaatctt tcttcttcaa catctggagg tgttatcaaa gcatttccag gcatattgat
    13561 taaaaaacca gcagataatt ttttccccct ttttttaagt aacttatcta tagtaaataa
    13621 actatgtcca gctaccccat tacaagtggc aatggcatat atatgagtat taatattaaa
    13681 atttaatttc tctacaaaag actttactat atcaggaaca tcagcaaaat aaacaggaaa
    13741 aacaattacc aatatatctt catttatgtc tatattattt tcttttctat gtatagctaa
    13801 gggtataact tctttacttc catttaacgt ttcttttaaa atatttgaaa ctactagcga
    13861 atttcctgtt ccagaaaaat aataaatctt actattcatt cttatccctc tttctatatt
    13921 tctacatcat caatagttaa aaagtctctt aaaataggat ttatatttac tttccaattc
    13981 tatccaaaat atcagtaaac aaaggcaatt cttcatcaga gatagattta aatgtctctg
    14041 ttgcatatat acttgcaatt tcatttaatg gttcttctaa ttctctacct aaatctgtta
    14101 ggaatatttg aaacttacgt ctatctttaa catctacttt acggacaatt aatcccttat
    14161 ttttcataac atctaaaatt cttgttgtat tagatttatc cttacatgat ttttcacata
    14221 aatctatctg agaaatccca tcttgctccc atagaaacct taataacgca taatggtcag
    14281 ttgttatatc atatccattt tgtttaaaca tcttttttaa atcatctttt acttttaacg
    14341 aagtacttac taacttagtt gtagcttcat atagtttctc aaaatctttc attttacctc
    14401 catttaaata atcccttgct cctcccatct atcaattatt cccttatatt gctcaatatc
    14461 tttaaaagcc attggcttta aaacagcatt tatagcaata aaattataga tttgagaaag
    14521 cacaggtgga tttttctttt cctctatgca ataaataaat tttttagata ccttttgtat
    14581 ctttgattct actttttcag ttataggatt ttgattaaat ccagttttta tagataaact
    14641 accacaaaca taacctcccg aatctaagac catggatttt ataaaagaaa ttgcttttga
    14701 accacgtgca ttttcatatg tagtaactgc tatacatggc ttacgataca ataatctttc
    14761 aagcatcata tgtgctctat ctgtaaacac cttgaaaagt ccagttacac tacttccata
    14821 agttgggcta ccaaatataa caccatcact cttagtaatt atatcatgaa tatactctac
    14881 tttatcattt tgattgatac acttgcccat tttataacaa tttaaacaac caatacaata
    14941 tgatggattt acctcactta aatcaaaata attaattttt acatctggta ttaatctttc
    15001 tatatcttta tacaagtaag ttaaaacttt tgatgtagct ccattttttc tagggcttcc
    15061 atttataact gtaattatca tttctatatc tcctttttag ttgttatatc aacaataagt
    15121 atatcaacta taattttagc tgtcaatata aaccatttat ttaaagctat acttacacag
    15181 cttaaataaa agttttattt aactacatgt gcttatttaa tcaactataa atgaattatt
    15241 tttaataatt gacaaactaa attaatgata atatacttta tgtattacaa atgtaagtag
    15301 aaggaggtat agtatgaaaa agttaccaca tatttcagaa gcagaatatc aagttatgaa
    15361 aattatttgg aaatatgctc ctatcagcac tactgaagta attgaaaagt tggtagaaac
    15421 aagtacatgg agtcctaaaa caattcaaac aatgttattg agattagtta aaaaaggagc
    15481 tttaacatat gaaaaaaata gcagagtatt cgtttatact ccacttgtta aagaagaaga
    15541 atacgtagct acagaaagta gttctttttt aaatcgtttt tataatggta ctcttaattc
    15601 tatggtacta aactttttag aaaatgataa actatctgaa gatgatattg aggaactgag
    15661 agaaatctta aataaaagga cgactaaggg ggataaataa atatgtatgc cccattcttt
    15721 atacgatttt tactcagtac attaacctta tcaatattaa ttgctgtaat tttacttgcc
    15781 aaaaaagtat ttaaaaaaca tatttctgca aagtaccaat acaatattgg atttatattg
    15841 cttataatgt taataattcc atttgtccct cttaaatata ttaatcttgg aaatgtattt
    15901 aactatttat atacttttaa tcaacttgga agcagtacta ataggacttc tatgataaaa
    15961 aatagtccaa attctataat taataactca aatgcattaa acgatttttc agtatcccta
    16021 aaccaagtta attttgatag tttaaatatt ttattactaa tatcatggat aattggaatt
    16081 ttaatcatga tagctattac tattcattgc aacattaaaa taaaaaatat gaaaaaatca
    16141 atacaaattt taagagatga ggagatagta tttatatttg aaaagtgtaa aaaagatatt
    16201 ggtataaata aggattttat actatgcaaa tcacctatta taaatacacc tattacattt
    16261 ggatacttta aaccacacat tatattacca gataagtcta tatctaaatt atctttaaaa
    16321 gatataaaat atatcttact tcatgaatta caacacttta aaaataaaga tatactgata
    16381 aattatataa tgtgcttcct tcaaatatta tattggttta atcctcttgt atggtatgca
    16441 tttaaagaaa tgcgtataga ccgtgaaatt gcatgtgata tttcagtttt aaaaaagctt
    16501 gataaagata gtcatataga atatggacaa actctaataa actttgtgga taatatttca
    16561 agaccatcaa atcttacact tacatcagat attggtgggt caaaaaaaca aataaaaaag
    16621 cgactattga agataatgga ttttcataat gaatcaaata cgttaaaatt caaaagcatt
    16681 ttaattttta cattgatagc tttaattgta tttggaaata ttcccaaact atctattatg
    16741 gcagatatca atgaaaaata tagacctaca aatgaacaaa ctgtgtacga ggacttaagt
    16801 cagtatttta aaggttttga tggaagtttt gttatgtatg atttaaaatc caagcaatat
    16861 catatttaca ataaagaaaa aagtaaaaca agaatttctc caaattctac ttataagatt
    16921 tatagtgcct tattgggatt ggaaaatggg ataataacaa gaaataattc acatatccct
    16981 tgggatggaa agcatcattc taatagttct tggaatgaag accaagactt attttctgct
    17041 atggaaaact ccgtaaactg gtactttcag actctagata aaaaaacagg gctaaaaaac
    17101 atacaaactt atcttaggca aattggatat ggcaattatg atgtttcagg tggtatctca
    17161 aacttttggc tagaatcttc attaaaaata tcacctattg aacaggtaga attacttgaa
    17221 agtttttata caaataagtt taaatttgaa gataaaaata tagaaacagt aaaagattca
    17281 ttgttaatat caaaaaagga tggggtatca ctttttggta aaacaggcac tgccaatata
    17341 aatggaaaag atgtaaatgg ttggtttata ggttatgtgg aaaaaaatga aaatacttat
    17401 ttctttgcta caaatattca aagcaaagat tattctaatg gtagttcagc atctaagata
    17461 acattatcca tattaaatga caaaaaaatc tataatcctt aatatattta ctgtacattc
    17521 taaaaaattc acataatttt catattaatt atcaattaaa ttcatataca aggcttatta
    17581 tataaacata gaagatagta aaactagctt aaaaacataa tataataccc tatacaaata
    17641 gtggcaactc tccctcacat cctcaattgt tgccactatt taattttttg tatgaagtta
    17701 aatatatttt catcatttca acaatttggg tgcaatacaa tctctatttt gaaacaacct
    17761 ctttaattgt tttagagttt atgaattatt tctcatactt taaaaagatt gaattactct
    17821 tgataatctt cttttgtagt cattacatat cacctactac tcaacacata ttctttgacc
    17881 tatttttaca tccttgttta tatccaaaat atttaccttc ttaccatctg gaaacaatac
    17941 cattgttaaa gtagactttc ctaattcatg tattctcttt aaatcaacat taacaagaac
    18001 atctccctgt ttaaccatat ctccttgttt aacagcaata tcaaagccat ctccatttaa
    18061 tgcaactgta tcaattccaa catgaattaa tacttctcca ttagttcctt taatcccaaa
    18121 tgcatgtcca cttgaaacca ctgtttctaa tttacctgaa ataggagaaa caatttttcc
    18181 atctataata tcaatagcaa aaccatcacc taacattttt ttagaaaatg tttcatctgg
    18241 tacattttct aatgcaacaa ttttcccctt agctggagac acaaagtttt cattattttc
    18301 attaaacact aattcttcgt cttcatcttc atttatagct tctttaggca ctccaaataa
    18361 acatgttaca ataaagcaga atgcaaccac aaatattgat gctaataagt atgtgattaa
    18421 attatatcca tcatagatat acattaaata agaaggtaaa actgctaatc catatgaatt
    18481 agattgaata tgtaaaattg ataatattgc tgctcctaaa cctgaagatg cacacataat
    18541 aatcaaaggc tttaaattat atcgaagctg aatcccaaat aaaacaggct cacttatacc
    18601 aaaaagttga gatagcattg ctccaactgc agtttgttta acttgattat ttttagattt
    18661 tttcataaat gctagacatg ttccaatatt cgcaaatcca tacattgcac aaagagtaat
    18721 aagggcatta aatcctgtat ttgctaatag tgatgtttca atcattacat aagtatgatg
    18781 taagccagta ataacagcta atggatatgt aaatccaata ataaatcctc caattcctaa
    18841 aggcaaatca atcaataagc tgatgatatc aaccattttt aattctattg tatgcattat
    18901 agggcctaca cctaacaaaa taactaacat tgttattagc ataacaaaaa atggtgtgaa
    18961 aataaggtct aatacattag gcatatgttt acggaaccat ttttcaagat tagctccaat
    19021 aaatgctgta acaatagctg ttaaaacact tccttgacaa cctacaatag gaataaatcc
    19081 aaatgccata attgcttcaa tttcatttga tgggtcagca acagaatatg cattaggaag
    19141 tattggggaa actaacataa gtccaataac aagtccaata acaggtgttc ctccaaatac
    19201 tttaaatgct gaccatacaa taattgctgg taaaaaagca aatgctgttt ctgtaagaac
    19261 cgaaacaagt gtctgaatat acaatggaat atttgcagaa gacattccaa ataatccaag
    19321 tacattatca ttaaacaaac aaccctttaa tcccaagaat aatcctgttg ctgcaataac
    19381 tggaataata ggaacaaata tatctgctag agttctcata actttcttaa atccagtttc
    19441 attattttta actaattcat cttgttcttt ttttgataaa gtttttaatc ccattttttc
    19501 aacttctgca taaactttat taactattcc tgttcctaaa ataatttgat actgtccaga
    19561 agtaaagaaa acccctttaa cctcatcaac tttttcaact ttcttatcat taataatttc
    19621 tctatcttta accatcactc ttaatcttgt ggcacaatgt gtaatagaaa taatattatc
    19681 ttcaccaata attttaataa gctcatttgc tattttatta tatttattca tttttctacc
    19741 tcactttcaa taatactgca tgaaatggtt tcaaacgcac tgttttattt aatagattta
    19801 tatcatctaa attttggaat acaatatcat atttttcttc tactcttatt tctacaggtt
    19861 tatcagaaaa attataataa caatcaataa ctaccaaatc taattgcctt ctatacgcaa
    19921 taatatcatc actttctata tcaataggtt ctattgaacc ataaatcaaa caatcagaat
    19981 actcactatc ttgtctcaac tttatcatct ttttataaaa ttctaaaata ctctctgaat
    20041 tgttaatctg actctgtgca ttaatatctt tataattttc aataaccttt aaccatggtg
    20101 ttgtatctga aaaacctgca ttttctttat ctgcccatgg aaatggcgtt cttgcattat
    20161 ctcggcttct taaattcaca aattctaacg cttcttcttc tgagtaacct tcatctattg
    20221 aacgatagta ttggtcaatg ctagataaat cattaaaatc atttatggta tctcttttaa
    20281 aatttgtcat tcctaattcc tgtccctgat aaataaatgg ggttcctctt aaaaagaaat
    20341 acattgctcc caacattttt actgcatcat cattcctttc gtattgcaaa agatactttg
    20401 ttgtagctct tggttggtca tgattttcaa taaaattagc tgcccatcca tgttcctgaa
    20461 catacatttg actatctaaa atgcattttc ttaaatctgg aattgtccat gaaattcttt
    20521 taaaccactc actaccagaa gcaacatcta agtcagcatg tttaaagtca aagaccattg
    20581 aaaagaaacc atcttctcca ataaaatcac ctaactggtc atagctaata cctgctgctt
    20641 cagcaacagt catacaatca tattttttaa acgtattttc ttttaattca taaagcattt
    20701 tttctattcc aggctgattt ctaactgttt ttgtacattt aacaagacca tcaacaccat
    20761 cactttctaa actttgaaaa cttaaattct ttttgatgaa tgtaatagca tcaactctaa
    20821 atcctgccac tcccatttga gaccaggtat taatcatttg gtaaagttca cttcttaata
    20881 ttggattttc ccaattaagg tcaggttgtt tttttccaaa agaatgaaaa taatattcgt
    20941 ctctattctc taccttttcc caaacacttc caccaaaaac agacctccag ttatttggca
    21001 aatcgtttcc ttttttaaaa atataataat catgataagg actttctgga ttttttaatg
    21061 cttcttgaaa ccaaggatgt tcatcacttg tatgattaag aactaaatct aatataattt
    21121 tgattcctct atctttactt tttttaatta actcttttaa atcttccata cttccaaatt
    21181 cacttgcaat atccaaataa tcactaatat catacccatt atcatccata ggtgatttaa
    21241 aaattggaca taaccacaat gatgtaatac ctaaatcttt taaataatct aaacgagaaa
    21301 taattccctg taaatctcca attccatcat tattactatc ttgaaagctt cttggataaa
    21361 tttgataaac gatttctttt tgccaccatt tcttttccat aaattactcc tttttatatg
    21421 atatttcttc tttttaattc tttataaatt ccattatcga atatgtcctc acatatagat
    21481 gtcgctatat ctttaagttg ttgatgacta tttttcattg caattgtaac gtctgatgct
    21541 tgaaacatga caatgtcatt ttgaccatca ccaaaacata tggtttcttt ttgagttact
    21601 cctaatcttt cttgtaatct cttaattgct ttgcccttat gaaaatcttt ttgaataatt
    21661 tcataatact gactagaaat atctctctgt gctaattcca ttttatcttg tagtatatct
    21721 ttcacttcgt caaatacctt ttcatttgac cataaacaaa ttttatgtat atcttgtgac
    21781 ttatactctt caatgttatt ttcatatgtt attttttctt ggatatgttg tttatttata
    21841 caagaattag ttcctttcaa ctgattcatt gtttcaaata tttctttggc tttttgattc
    21901 atgaaaactt tttcctgact ctcaatcgaa aaagcaactt ctctattttt taataaacaa
    21961 acaacttcct ttatcaatct ttgattaaaa gattgattat aaagtaattc accatgatat
    22021 tgaatgtaat tacctcctcc tgcaatatat ccatcaactc ctaatgataa aacatcatct
    22081 tgtattgtac ccatacttct tccagtacaa ataacaacac tgcaatgatt cttttgacat
    22141 aaacgtatag catgctttgc agattctggg attccatata cctcatctct aagcgtacca
    22201 tctatatcaa gtattaataa tttgtacaat ttcatcttcc tttcttacta tcaaaatcat
    22261 aatagcattc aaaaaaaaag atatcaatat tttgatatct tttaaaaaat atgttcattt
    22321 ttagcacaaa ttttcatttt cggggaatat gttcactttc taaatacatc ttgtatttaa
    22381 tataaagcag ttcaattaaa ataaaataag gtgttgttac atcgtagtcc agttgtttat
    22441 ctacagaaat agatatagat tgaatataca ggttttcatc acataatctt gctaaaggat
    22501 tacttatcat ttttgtaata gaaaccatat aaattccttt cattctcatt tctttagcaa
    22561 aatcaataat tgtagatgca tctccattca atgaaataaa gaaaatcata tcatctgttt
    22621 ttataaaatg attaagtgcc tgaatcatat cataaccata aacatcatat ataattttcc
    22681 ctgttggtaa gaaaatcctt ttcatctctt tagcaactct ttcctgagaa taaccttctc
    22741 caaagacgat tatacgattt gcctttttca ttctttcaaa cataatcgaa caatccttct
    22801 tctctatatc atttatcacc ttattatagc aatccattaa ttggtgttta ctttgatttt
    22861 tatattctac ttctgtttca tttaagcgta taataactct taattcacta tatccaggta
    22921 attgtagttt ttgagcaaaa cgagaaagtg cacttgtaga tatatggtat ttgtctgcaa
    22981 attcatctat agaaagttta atacaatctt ctttatgatt aagaatacac tcacagatat
    23041 acttatcatt tgctgtaaat gaattataat atttattgaa aagtccttca attttcattc
    23101 ttttctcctt ctatactcta tgtaattcat atgaagcaaa tctaatagag cataaaatgc
    23161 tcctacgact tcataagata tagcttgtaa tgtatcaagt ttttgagttt caacatacag
    23221 attatactca caaagtgttg caattgtatt attatcaagc ctagttaaag aaatcgtatg
    23281 tatatgattg ttgataaatt ttgcaatttc aattcctgct tttgtttctc cagacaaaga
    23341 aatgataata aataaatcac ttttttgaaa tgattctctc ataaattcaa tttcacctat
    23401 atcaaataag tcaataacac atttacctac tgaaagaaat attctcttta tttcttgagc
    23461 taaacttttc tgttcattac ctgtcccata aatataaatt gtttctgcat catatattat
    23521 tttacaaata tcttgaaatg atattttttg taattcattc attaatagtc gataattctc
    23581 gcaaacatca tgaaaattat tatcattatt aacaaattct agttgattat ttttaagtaa
    23641 atactttaaa tcagagaatg aatctaatcc aatttttcta caaagcctta ataaagtagc
    23701 tctagaaaca ttacattcat ttgcaatttt ttcacacgaa aaattacggt attctctttg
    23761 acaaatcaat atttgtttaa taataatttt atcattttca tttaaattat cataattttg
    23821 gttaactaac gattctaatc tcattttttc cactccctaa atattatttt aatatacatt
    23881 agttgctttt tctagggaag agttttaaat aataaggata aaataatcat ttgattttat
    23941 ttttattaaa ataaaaataa cctgctaaaa gcaggcacca aaaagtactt atcctttcga
    24001 acatgtactt tagatattgc tttcttcgga agcttcatct cttcatactc taaaatagag
    24061 ttttttctaa ctcaaaagta ttttccatgg tagaaattac tttaattcat ctgccattta
    24121 ttttttatct tatttaataa ttactactac ttgaatattt acttctttaa gtaaatttca
    24181 tagttaacaa agctaaaatt attattatag atgttaaaat acaaaattgt aagtattgtt
    24241 ataaatatgc ttacagctac aaatatacat gatatattaa aaagaagctg taaattatta
    24301 tcatacatac aagcaactac gcctctatta atccatgtta atttttatta tttaatacaa
    24361 ggagttccat gagacataca tatccattca tattttaaat tatccaattc tcttatagat
    24421 ttcatcatct ttttataatc ccaattccat ggattaggat aaggaactat atttccattc
    24481 ttatttttga ctaaatcacc tgcaaataat acatcttcaa aaatcatgca gacatgccct
    24541 ggagtatgac caggagtatg gataattttt atattcccaa tctgcatatt ctctccataa
    24601 ggttcaacat ccttaatgag ccttctggaa attgcctttc caataaactt cttaaaccca
    24661 ggtctatcct tctctcctat aataaaaggt atatcctcta catgagccca tacttttgct
    24721 cctgtttttt ctttcaataa tttaatatta gatatatggt caacatcatg atgagttaat
    24781 aaaatatgct taatatctgt aagtttaatg ccaagtgttt ttaattcttt aattatattt
    24841 ttccccataa aaggaagacc tgtgtctata agaataattt ctttatcaaa cacaataaaa
    24901 caatttgagc cacgagtact atctaacatt aatacatttt cttttatctt cataaataaa
    24961 ttccccttat ttggtatttt acctatataa ttaattaata aagttataaa tagttataat
    25021 acttgatatt aattttattt tttattaaag tatactatag gtatacaaat attatattta
    25081 cataaatata ccatcagtat acttttttat caatagaaag gaataattta tatgtcagaa
    25141 ttatcaaaaa gagataaaga aaaaatacaa agagaaaatg agattattga taaggcagaa
    25201 aaattatttt gtcttaatgg atttgataat acaactatga atgaattagc aaaagaagtt
    25261 gaatatacta aacgcaccat atacaagtac ttttcatgta aagaagattt attctttgct
    25321 gttgtcttaa gaggatataa aagactttgg gataatgtta aaatagaaag tgcaaaaggc
    25381 aaaacagggt ttgaaaaaat aaaactttca tactttgctt ttcataaatt ttactctgta
    25441 gaaccttctc tattatgcct tatgggaatg attggtattg taaactctga aaaatcggat
    25501 actgatatgc tgtttaaaga aaagtttttt tcttttaata aatttatgtt tgatgaaata
    25561 caaggaatgt ttgaaattgg aaaaaatgat agaagtatta ggcatgatat tgaaatacct
    25621 actcttatgt attcatcaat atttacactt acaggatttt ttaatttact atctgtaaca
    25681 ggaaaatcat acctgaacaa ctttaatata gatgaggaaa aatttattga aacaactctt
    25741 gctcttttaa tagattcaat taaagcataa acctaagttt ataaattaaa gtttatatac
    25801 ttaaatgata attttttcta tctctaatat gaaaaatatt aaacataaaa ctaataactt
    25861 agttaatgga ataactaaat tactgaaagg attaaaagta aatggctaaa atacttcttt
    25921 tagaagataa tgatacaatt acatttggaa taaaaacttc ccttgaaaag aaaaatcaca
    25981 ctgtatgttg ttataatacg attgctgaag ctaaaaaaaa ttttgataaa actattgatt
    26041 taattctgct tgatttaaat ctacctgatg gtattggata cgagttttgc caatatgtta
    26101 aaagtataga agatactcca gtgatatttt tgacagtccg tgatgatgaa aaagacatta
    26161 tacgtggatt agatatgggt gcagatgact atgtggttaa accgtttttg ctctcagtac
    26221 ttcaatccag aattaatgca gttcttagaa gaactcattc aaatactaac aatactatta
    26281 catgtgaaaa tataagcttg aataaggttg aagtcaaagc atatttagat aatgttgaag
    26341 tacctttgac agcaggagaa tataaacttc ttctaagtct tatggaaaat aaaaatagag
    26401 ctatgactcg tacaaagtta ctagaaatgc tctgggatgt agatggtaat ttcgtaaatg
    26461 ataacactct tactgttaca ataaaacgtc ttagagaaaa attaaaaaat ccaccttgta
    26521 taaaaacatt aagaggcata ggatatagaa tggaggacaa ctagtgaaaa ctccaagaaa
    26581 tatcaacagt acagttgttt ttataatatt ctgcttaatt gtaacttttt tattgagtgt
    26641 aattctaaca actataactt cttttaatga atacaatgta ctgtctaagg ctcttggtgc
    26701 atcatatgag tacaatccta agtctacaga aagtatacta aaatcactga aaaattctag
    26761 tgataaggat tttaaaacag gaaatgaaat tttatcaaaa catggatatt ctaaaagtac
    26821 attttggagt aaaaactttt tttattttct agtgatatcg tttggtataa tttcaacaat
    26881 ggctacagtt gtatatataa tcaaacatag aacaaataag caaaaactga gtagaataaa
    26941 caatcttact caatacttag aagaagtgaa tttaggaaaa gagtctgtac ttttacgatg
    27001 tgaagatgaa ttttctcacc ttgaagatga gatatataaa acagttgggg aacttcgttg
    27061 ttcaaaagaa tttgctttaa aagaaagaca aactctttca gataaccttg cagatattgc
    27121 tcatcaactt aagactccaa ttacctctat gtcacttatg gcacaattac tttcagaaaa
    27181 ttgtccagat gaagaaatag attatattaa cagacttaca aatcaaatct caagacttga
    27241 gagactagtt tcatctttac ttacattatc aaaattggat gcaagtactt taatatttga
    27301 aagcaaactt atagatgtac attctcttct tacttatgca attgagccta tagaaggttc
    27361 tttaagagaa aaacatcaga catttactat cgaggcttct ccttcggtag aatttgaaat
    27421 cgatattaat tggactttag aagctctttt aaatattctt aaaaattgta gtgagcatat
    27481 agaagatggt ggatatatag ttgcttatta tagtcaaaat cctctatatg ttgagattat
    27541 catagaagat aatggtaagg ggtttgctca ggaagaactt ccatatattt ttaatagatt
    27601 ttatcgtggt aaaaatgcta gtaaggacag tattggtata gggcttgctc tatcaaagtc
    27661 tattatagaa cgtcaaaatg gtactctaca tgcagaaaat tgccataatg gtggtgcaag
    27721 attcataatt aaattttaca cataaaatgc tggattatag gtaaaatacc tataatccag
    27781 ctatatctat attctatgtt atctattgtt ttagtaatat acttctattt acatttaagg
    27841 ttttattaaa caaacaactt aactatccct atacttgtaa gtagtcttaa atcgaaacct
    27901 ttaaataagt tcaaatagat tttgtttata ttgtttcatt ttttatagat tctactatat
    27961 tttcattctt caactgcttg tatcctgtca tatatgatgc tattacaata aaaagtatca
    28021 atatagtaaa acctattatt gtagatatag gtgcaaatgg taaatactct ataaaatata
    28081 tttcatttat acgtagaaaa gcatacacta ttcctatttg cacaggaata ctaagtagta
    28141 atggtgtaat tcctaagaat aaaccttcca aaataagcat cttttttatg ccttctggag
    28201 ataatccaac agaacgcaac atagcaaact cttgtcgtct acttctaata tttcctgaca
    28261 ctgtagcaag tacatttgat aatccaatca tagccaataa acctgtaata aagcctacta
    28321 ttaaattcat ggtctttatt ccactagcct tttgtttttc actttctaaa acatcctcaa
    28381 tactataatc tcctgaacca tagtctttat tataaatgga ctctaatttt tctcttactc
    28441 taggaatgtc ctcacgatta tttactttaa atactcctat cagcctattt gaacgtaacc
    28501 tacttacttc atcataattt ttagatattt cctgcaccat agacattgga actatttgca
    28561 ttaatgtata ctttgaacta gtatctacta ttttaggaag tgtgcttgta atagctccca
    28621 ctttcatgtt gtaagtataa tcccctttat cttcgtcata tactttttca tttaatttaa
    28681 tgttatctcc tattgaaaga tttaaataat ctatatatac tttatttcta cgtgtactac
    28741 gattaatatc ttcctcctta ttatatacaa ttgaggttgg attttttgta tcataaaata
    28801 actttgggtc tacagaaatt tgcctacaat attctgtaaa actcttatca tcaagcccaa
    28861 ttatatttga aattatacga aacttgccat cacgttctat aggagagtac tttttagatg
    28921 aagctatatt tttaaatcct ccatttttcg caagttcttt tgaagcatct ttttcagtca
    28981 accaagtgga agcagacatt ttttgataat atagtgaact ttttacacct tctgtattat
    29041 ctaatttttc aatgaaagca gactctacag gttcactatt ttccaaataa actgctatat
    29101 ctctttggtc cttataatta tcacttccat aaacagcttt tgctccttct tgatttgcaa
    29161 ttatatgcaa aaaacatgta agtaataaaa aagataatgt taaagatatt gttgcagtac
    29221 gataagattt tttacgagca cgtagtgcgt ttgatgcaag ttctccttct attccaaata
    29281 tctttcctaa tgaatattta tgcgactttt tgattttaac actatctcct tgtcgtattg
    29341 cttctatagg agatatcttt gatacttttc gtgctggtat aagtgcagaa atccagactg
    29401 ttactattgt aagtaaaact gctggcagaa aagcaggaat accataagta aataccactt
    29461 ctggaacatc agtatgataa ttaactgaat taacataaac tataagccta ttacacaata
    29521 accatcctaa aaataaacct attggtaatg gtataattgt cagtaaaaag ccttcaaaca
    29581 ctacagacct tttaatttgt tttggtgatg caccaacact agataatatc cctaattgag
    29641 taagtctagc atttgcagac agtgaaaacg cgttgtaaat caccattaca aacacggcta
    29701 ctgtaaaaat tgctattgta agatacataa taggagttga aaattttgat atactactca
    29761 tagatgcttt ctgttctggt gaaaagacta acatctttaa aagataatta ctattgtatt
    29821 taagattgta ttttccatat tcattagtct tccatcccaa tgatttagca agttttggaa
    29881 gttccttata cgtgtctctt atatttttaa atcgcaagta cacagttata ctatcattag
    29941 gttttatact tgttttatca agatatccaa gagctgtata tgcaggtatt actgatgaag
    30001 tagaaacatc tacctttcct actattgtca atgtagcctc tgaaattttt ttaaaacttt
    30061 cccctttcga ttttggggct atgggttcaa gaggctttcc atttaggatt ctatctccta
    30121 ttgggagagt aattttatca ccaattttta cacttggatt attctcaaaa tattgttttg
    30181 ataatgcaat ttcattagca ttatttggtc gacgaccctc tattatagta tccttttctg
    30241 gcatagaatt ccaatactct gatgtagcat ttctataaat tatataatct cttttctcat
    30301 cattaatttt gcctacatac cattgtcctt ttaccattac atcatcaact gaagaaaaat
    30361 tttcaattaa tggtaagtca cttccataag tatagtcaaa caattcacca tgccagttac
    30421 cttctttctc aattgaaagt tttataaaat ctgtccacat ggtatacaac agtccacaca
    30481 aagaagacat catggttgta gtaagtaata tagctaacat tatagctata ctactacgtt
    30541 tattgcgtcg tacaaagtct aaggtgtatt cttgaagaat attcatttct tcaccacctt
    30601 ttcagagaca atcactccat cttccatagt tacaatacga tttgcctcta gtgctatttt
    30661 ctcatcatga gtaataagaa gtattgtctg attaaatcgt ttatttgata agtttaacaa
    30721 gtctacaatt tcttctgaat tttttctatc aagattacca gttggctcat ctgctaataa
    30781 aatagctggt ctatagatta aacttctagc tatagatacc ctttgttgtt gtccacctga
    30841 aagttgactt ggaagatggt ttaatctatc agataatccc aatatagata caatctcaga
    30901 gaatctatcc tcatccacct ttcttttatc aagtaacatg ggtaataata tattttttct
    30961 aacatcaagg gtaggtataa gattataaaa ttgataaata agacctactt ttctacgtct
    31021 aaaaactgca agagcttctt cctttaaact tgatatttct gtatcttcaa cataaatctt
    31081 tccagatgta ggtctatcta ctcccccaag cagatgtaac aatgtagatt tgccagaacc
    31141 agatgctcct acaatggata caaaatcacc tttttgaact gataaattta cattattaag
    31201 ggctgtcact ctggtttgat ttgaaccata tatttttgtg agattttcac attttaatat
    31261 ttccatatac aatcatctcc tttatggtta aattttagca acctaaaatg acattatagt
    31321 gacatgaata caaatataaa ttactattaa aaatattgtt tagtttttgc acattattta
    31381 aaattataat ataaaacaaa aactatgcaa tatattttat ttatactttt aatatattac
    31441 atagtcttta aaaggtatta actttaaatc tataggtatt caatttaata atcaatagca
    31501 agtattttat gtactaaagg agttttgctc tatatagact caatttagta aaattattta
    31561 ataaatatta cagactatag gtaaataata tttacaaata aaattaagtt tattccctta
    31621 cactataata tttcttaatt atattaattg caatttcctt tacctttggc cctgtaattt
    31681 ctttattgga atcatctgat ttaatagcaa aataataggt gtcaccttca cgctctagca
    31741 taccaacaaa ccatgcatcc acacgtttat ttttttcatc aaatcctgtt ccagtttttc
    31801 catatacatt tatgttttta tcatttacat ctattttcat aatatctctt aaaatattaa
    31861 tatgctcttt tttaaagttt gtatctccct caaatatttt tgccattgtt tgcacttgtt
    31921 cttttggaga tatttgtaat gaagattcta accaaaatcc atttaaatgt ccctttccat
    31981 tttttaaatc tccttcccat tcgctaatat cgcaattccc atactttaaa tcatctaaaa
    32041 tgttttggac agatttagca tcaactttat ctatcaattt tttgtagtac caaacacaag
    32101 actccttaaa tgcctcttct aaactaagat ttttattcca atttttattt ggatactctg
    32161 taccatcata tcccataact gattcttttg agtttattac ccctttttct aaaccaatta
    32221 aagttgaaac tattttaaaa gtagaacatg gtgaccttct tgtttcaatt aattctttgt
    32281 tatatatatt atattcttta tttttagtat tataaaaaat agctcctcca ctaatacctt
    32341 caaaacaatc actatagtca accatattaa ttttttcttt tttgttttta ttattgtttt
    32401 caattttgga tttatttaat tcagacttat ttaattcaga ttttatataa ttcttatcag
    32461 ttttttgatt tatatcgtta tgttttttcc ctacataata cattaccata actacaccaa
    32521 ccaataagat agctatccaa ataaaattct tttttctttt cataatttat atcatctccc
    32581 atatttaaat ttataaccta ctattaaaat agtagacatt atttgtaata tactccaatc
    32641 taggttctac ttataagaaa caagactaaa tgaatagctg tatctcttat attttcttca
    32701 cctagtacaa ttatgttgat taaacctatt gcgaagataa ttaagtaact attaataaca
    32761 tcctgtcctc cttttaattt gtgatataat aattgattcc acatttaaaa cttactattg
    32821 taatatttaa atattacaat agtaagtttt aaatgtcaag ttagtttggg aaagttattt
    32881 taattgtata attgttatta catttataaa atatggcatg tttaaaatta ttttgctatt
    32941 gggaggatta agataagcag tattataaaa gctataaatt ataaaattta atatataatt
    33001 tatagctttt acttttacaa atattgtttt tattgattta ttaatgaatt ttattgacag
    33061 gttatattta tataagtaaa cacttacata ataagtatat aatctacata tctataagtt
    33121 gagtcaattc accagtatat gtcaacttaa gtttatgcat agctcgtgta caagcaatat
    33181 ataacaaact gcggtcataa tcactataat atgttttatt atttactgaa ggtacaataa
    33241 cttcatcaaa ctctaaacct tttgacattt taatagaagt tatggatact ccttttgtaa
    33301 agctagaact ttcagaagat attaagtgaa cactatattt ttctcttaac gcatcatata
    33361 cagctttagc gtcattatct gtctttaata taataccaag agtcgcatta tcactctttt
    33421 taaattcttc aatttctatt tttatcttat ttatttcatc ttgctcgtta ttacatttta
    33481 ataaagctgg ttcttcacca tgtcgttcaa taggctctaa tgaagatatg tcttgaatct
    33541 ttttggcgaa attaataatc tcaaaagtag aacggtaact cttattcaac ataactagtt
    33601 ccccatcatt atatagctgt ttcatatcat ctagtgtatg agtgtgatta ggattaacca
    33661 attgtccaaa atctccaaga atagtctttt tacacttaaa aagtaagttt attacagcat
    33721 attgaatagg tgtgtaatcc tgcatttcat caataacaac atgtcttata atcttatctt
    33781 cttgtatacc ttcatatgca gcatgaatat atataaatgg gtatacatct gaccattcaa
    33841 gagttttttt tgttgccatc acaaacatgt ttgatatatt catttgttta aagaaatcct
    33901 tatatagtgt aagagtattt tttattgtca acattccatt caaactttta agtatacttt
    33961 ttacctttgg taaatcttct tccataatat tatctgattc aaatttataa tgaatgtctt
    34021 cagctacctt ctctaatctc ttcttaactg gatatctgtt atatgcctca aatctactct
    34081 gtatccagtc acttttagct gtaaaacttc caaaagtgta gtcttttgga ataaaaatct
    34141 tatttggcat ttgttttata taatcatcaa taagttttac aaaatcaagt gtagatttaa
    34201 aacgcactct ttcagaccat ttagcatcat ttatttctaa tgggtctttc tcactttcaa
    34261 aattaataac tctatctaat tgtacctctg ctatattttc aaaacttaat tcacataatg
    34321 gctcttctcc aagctcagga aggacattgg aaatatagtc tccaaagact ttattaggtg
    34381 ataggataat tacattattt gctgatattt tatctttaaa tcgataaaga agaaaagcaa
    34441 ttctgtgtaa tgctatagat gttttcccag aaccagctac cccttgaata atcagtgtat
    34501 cagctttatc atttctaata atttgatttt gttccttttg tattgttgaa atgattgact
    34561 tcattttttc atctgaagtg tgactaagtt ctttttgaag aatatcatct tgtatattaa
    34621 tggaactttc tagtgcatat tctaattttc catttttaat tttaaattgt cgttttcttg
    34681 ttatttctcc atccacccat cctattggag catcatatcc agcttttccg atttcataat
    34741 cataaaacat actggcaatt ggagaacgcc agtcaagtat tataagctca tcttcataat
    34801 caaatgcaaa tctaccaata taatacttag attcatcatc catatctttc agcctaaaat
    34861 ctattctagc aaagtatgga gaatctttga ttttattaat cttatctcga acttttacca
    34921 taaaagcccc atagttatct atttgtttta gtccttgttc attttgaaac atttcatgtg
    34981 ggtctatttc accacgattt tttaccatat acattttagc gtccatataa tccttatcaa
    35041 gcttgtcaac aagattttca gctttcttaa aagcattgtc tagcttgtta tttatctcta
    35101 ttagatgtgc aatctcatct ggaaaaaaag ttaataactc tgcatcatat tcacacatat
    35161 tatgtttaga attagatgca ataatatcgc aatttactac ttgtttattt tcagacattt
    35221 aaatcctcct ttgttaatta agtacttaca ctgaatcatt atacttaatt gtgttttgga
    35281 gtttttaatc attcttgcta tttaacttaa atgttagctt tttagcttta aagttccttt
    35341 tttaaaaaac taaatctttc tatatttatt accataagta cacttacaag caaaatacat
    35401 taaaagcaga aatgctctta tatataaaat aattcaaaca aaaaatatgt ggtaaacttt
    35461 tcatttaatc tacaattaat tttgtctatt atctctttct tctacacaag gaagaataaa
    35521 tttttgagta ggatatccaa attccacaag caattctgct tctgcaaagc ccaacttctt
    35581 tatcatatct ctatgtcctg tatcagcttt gtcaccctct cgaaaggtag taattgaaat
    35641 atctgtatct atcaagaaat cttcaataac tttcttcaaa aacacttgtg caatgtctga
    35701 attacgatac tgtggatgta ttccaaaaaa atcaatactt ctagtttctc tattaaacac
    35761 catatttgca atagcgacat tattaagttt taatattaaa gctctttctt ctaaaatata
    35821 ctgttctaat tgatatatat attcttcctc ttgcaaattt ggaaatccat ctatgactaa
    35881 tctcactaaa ttcatccata atggtatgtc agacatactt gcaagtttaa tttctttttc
    35941 caactctagt acattctttg aaaataggtt atcttttttc aaaacaaatc ttagctgcaa
    36001 aggataaaat tcttgttctt tcctatattt atttggagat tttttataca tagctttaaa
    36061 tatagaagtg aatgcctgtt gactttcata tccagctatc aatgcaattt caataattgg
    36121 tttctttgaa aataccaata attttgctgc ttctgtcaac tttctccgct ttatgtaatc
    36181 atgcattgtt agaccaacag aagttgtgaa agttctatgc aggtgatatt ttgagtaatg
    36241 aacagcatta gcaactatat ctaaatccag cttttctgat aaatgctcct ctatatagtc
    36301 aatagcagtt atgacattaa ttactttgta agacattgca gcttccctcc ttttatccat
    36361 gcaacattat atcaattatt acttagttat atttaatgaa tcttgctatt ttataaacaa
    36421 atttgttgtt taagttttca attcataaat ttaatatatt actttttact ttctgattaa
    36481 agactctaat cctttaattt tcaataactt ttcatttagt aaatctaaaa agttatt
//
LOCUS       DAMJYX010000028        35929 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJYX010000028 DAMJYX010000000
VERSION     DAMJYX010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.028"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            335..913
                     /locus_tag="PC479_001544"
     CDS             335..913
                     /locus_tag="PC479_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2804093.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="PC479_001545"
     CDS             952..1530
                     /locus_tag="PC479_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2804094.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="PC479_001546"
     CDS             1616..2245
                     /locus_tag="PC479_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2804095.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="PC479_001547"
     CDS             2235..4994
                     /locus_tag="PC479_001547"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2804096.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="PC479_001548"
     CDS             5081..6643
                     /locus_tag="PC479_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2804097.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="PC479_001549"
     CDS             6644..7780
                     /locus_tag="PC479_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2804098.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="PC479_001550"
     CDS             7844..8920
                     /locus_tag="PC479_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2804099.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="PC479_001551"
     CDS             8898..10136
                     /locus_tag="PC479_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2804100.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="PC479_001552"
     CDS             10190..11278
                     /locus_tag="PC479_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2804101.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11306..12097
                     /locus_tag="PC479_001553"
     CDS             11306..12097
                     /locus_tag="PC479_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2804102.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYTNIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(12212..12766)
                     /locus_tag="PC479_001554"
     CDS             complement(12212..12766)
                     /locus_tag="PC479_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2804103.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12962..14164
                     /locus_tag="PC479_001555"
     CDS             12962..14164
                     /locus_tag="PC479_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2804104.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14221..15201
                     /locus_tag="PC479_001556"
     CDS             14221..15201
                     /locus_tag="PC479_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF2804105.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15444..16397
                     /locus_tag="PC479_001557"
     CDS             15444..16397
                     /locus_tag="PC479_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2804106.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16387..17343
                     /locus_tag="PC479_001558"
     CDS             16387..17343
                     /locus_tag="PC479_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2804107.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17340..18095
                     /locus_tag="PC479_001559"
     CDS             17340..18095
                     /locus_tag="PC479_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2804108.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18187..19152
                     /locus_tag="PC479_001560"
     CDS             18187..19152
                     /locus_tag="PC479_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2804109.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     gene            19633..21492
                     /locus_tag="PC479_001561"
     CDS             19633..21492
                     /locus_tag="PC479_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2804110.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(21784..22383)
                     /locus_tag="PC479_001562"
     CDS             complement(21784..22383)
                     /locus_tag="PC479_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2804111.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(22486..23283)
                     /locus_tag="PC479_001563"
     CDS             complement(22486..23283)
                     /locus_tag="PC479_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2804112.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(23396..23501)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(23593..24579)
                     /locus_tag="PC479_001564"
     CDS             complement(23593..24579)
                     /locus_tag="PC479_001564"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2804113.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            25063..26793
                     /locus_tag="PC479_001565"
     CDS             25063..26793
                     /locus_tag="PC479_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2804114.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            26903..26983
                     /locus_tag="PC479_001566"
     tRNA            26903..26983
                     /locus_tag="PC479_001566"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26937..26939,aa:Leu,seq:tag)
     gene            27318..27833
                     /locus_tag="PC479_001567"
     CDS             27318..27833
                     /locus_tag="PC479_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2804115.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      28325..28429
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            28639..31113
                     /gene="gcvPA"
                     /locus_tag="PC479_001568"
     CDS             28639..31113
                     /gene="gcvPA"
                     /locus_tag="PC479_001568"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2804116.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            31113..32570
                     /gene="gcvPB"
                     /locus_tag="PC479_001569"
     CDS             31113..32570
                     /gene="gcvPB"
                     /locus_tag="PC479_001569"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2804117.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            32993..35350
                     /locus_tag="PC479_001570"
     CDS             32993..35350
                     /locus_tag="PC479_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2804118.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(35436..35753)
                     /locus_tag="PC479_001571"
     CDS             complement(35436..35753)
                     /locus_tag="PC479_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2804119.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT        13921 a         3893 c         6160 g        11955 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatagaaa tttataggga
    11401 aaaagaaata tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta
    11461 tggtatgttt atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa
    11521 taaatatggg atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga
    11581 taatgaaata ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc
    11641 cattgaaagt atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt
    11701 taaagttgca gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga
    11761 ttcaatcaaa gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg
    11821 cagaaaaata tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac
    11881 taaagaatta ggaatagaat atttttatgc agttggagat tctattatgg actatggtat
    11941 gcttaatatc tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat
    12001 agaaaattca tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc
    12061 taacatttta gacgagaact gcctacttaa tatataatga ctgatatata aataataaac
    12121 tagaaaaatc tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta
    12181 tcaatttata tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga
    12241 ctgagataca tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat
    12301 aaaaactatc tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac
    12361 cttttattat atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg
    12421 tatgaccatc tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa
    12481 gaagttcata aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta
    12541 ctactatatc agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa
    12601 gagcttttga tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt
    12661 gacttattaa gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt
    12721 tagattttct aagagatcta attttgcttc caacatctat attcattaga tacaaacccc
    12781 ctcagacatg attataacat ataatttata aagattaata aaataaaaaa aaagttttac
    12841 aatacaagta ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta
    12901 atattcaata tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa
    12961 gatgaagagt tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg
    13021 ggcagcattt agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc
    13081 aataatagct cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc
    13141 tgttgtatat gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata
    13201 tcaatgtaac attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt
    13261 aggttctgta ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt
    13321 accagattta aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa
    13381 ctttgctgct atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt
    13441 agcagataat cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg
    13501 attttttaga aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac
    13561 tgaaggagaa acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat
    13621 agcaatagcc attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg
    13681 gtttaaagct ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg
    13741 aaatcaatat ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt
    13801 ctttggtgat ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt
    13861 tgtagtaggt gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt
    13921 atactgcgga ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt
    13981 taagtttagt ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac
    14041 agcagcagct atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt
    14101 tggaacattt ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat
    14161 ataattttac gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt
    14221 atgattttcg atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg
    14281 gaaaaagatg tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga
    14341 gctatttttg taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa
    14401 caaattataa gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta
    14461 aaaaaatatt cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt
    14521 cttgaaggat taagccatat tggaagaaat atagatttac tagattattt ttataaagaa
    14581 gttggtgcaa gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt
    14641 ttaggggatg aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa
    14701 aaattgggca tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg
    14761 tctgttgcaa gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct
    14821 aaaagaaatc ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga
    14881 ataaatagtt ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg
    14941 gttgaccata tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt
    15001 gattttagtg attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg
    15061 ccaagtgtag atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa
    15121 aatagaggtt ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc
    15181 tttaaagata tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat
    15241 acaaataata tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta
    15301 gaagtttcaa ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa
    15361 tgataattat tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa
    15421 taaaagattt ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt
    15481 tgtcttattt ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca
    15541 aaaatcagca taaacttatg atatttatca tgagtagaat accaagatta ataagtatat
    15601 tgattgctgg tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata
    15661 aatttgtatc acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat
    15721 gtatgctttt agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt
    15781 ctcttgtagg gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa
    15841 tctttgtacc attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta
    15901 tcgcgtataa atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta
    15961 tgatacttaa aggaaattat gagatattat atatcacaat accccttata atattagcat
    16021 atatttatgc aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag
    16081 gtcttagtta caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat
    16141 gtgtagtcgt tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat
    16201 ccttgtacat gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga
    16261 tatttgtact tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta
    16321 tagggcttac agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga
    16381 atgtgaatga agcttagtct caatcaaaaa atttactttg taagtatatt actattcata
    16441 tcaatggcac tttttataat ttatggaata gacatgaatc atttagaata tgcattatca
    16501 caaagaatac caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact
    16561 gtagtgtttc aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat
    16621 tcattgtatg taatgattca aactataata gtattcatat ttggttcaag cagtagtctg
    16681 attataaatg aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg
    16741 ttattatata agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta
    16801 ggaatgatat ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac
    16861 cctaatgaat ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact
    16921 aatgtattgt taatagcatt tataataatt attgccataa taccttttat atatgatgaa
    16981 ataaagtatt tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat
    17041 tttgataaag cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca
    17101 gcacttattg gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt
    17161 tttaaaacat ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca
    17221 ttgataatag gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta
    17281 gtaataaatt ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga
    17341 tgatagagat aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct
    17401 cttttagtat agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa
    17461 gtacagtact atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag
    17521 aaggaaaaag cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta
    17581 aacaatctaa caatataact ttaaagctta caataagaga attagttgga tttggaagat
    17641 ttccatatag tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag
    17701 aatatatgaa gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa
    17761 gacaaagagc ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg
    17821 aaccactgaa caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc
    17881 tttgtgatga attagataag actatagtat tagtaatgca tgatataaat tttgcatcat
    17941 gttattcaga taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag
    18001 atgagatagt aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga
    18061 atataaatgg aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg
    18121 ctatatgatt ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat
    18181 attgatatga ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca
    18241 gtgtttgcat taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat
    18301 actatgaaga taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta
    18361 gtggtatttg attattcggc tttagataca atggatgcat taggtgtagc tgagacttta
    18421 gtaggacttc caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat
    18481 gcagatttag gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat
    18541 ttaattataa taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca
    18601 acaataagta caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac
    18661 aaaatagcaa aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa
    18721 aagaaaatag aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata
    18781 atggtaaatg aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac
    18841 aatagttttg gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat
    18901 attacttttg aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga
    18961 atagctactg gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat
    19021 ataataaaat ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca
    19081 tggtatgtaa atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa
    19141 gcggtaaatt aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata
    19201 aactaggagt ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac
    19261 aataatatta tctataataa gataaatcta ttcaaaatct aataaaatat gaagttatac
    19321 tagacaaaaa gaataactct taaatataat taagttatat aataatatat aattataatg
    19381 atataatttt aataggtggt ttgtaattaa aaagtttata aattatatta atagtaataa
    19441 tctataagaa cttatatatt agaaaaaaat ttttgtatat ataaaggact ctaaagatga
    19501 actatgtaaa acaaggttta tatattctaa ttgtatagaa aaatttattg taaacgcatg
    19561 tatattatta gaaaaaatag tttggaggta ttcaataaac ataaaaaaca atatagggga
    19621 taaaggtgtt ttatgaagag atttttgagg agaattatct tagttttatt tattttgttg
    19681 atttttataa gttttatttc tattaaatta attcataatg ttggagatta tggaaaattg
    19741 attaattatg ttgggattgt tcgtggagct agccaacgtc taactaaact agagatgaat
    19801 catcaaccaa atgatgaatt aatagaatat attgatgaaa tcctacagga gttgattaca
    19861 gggcatggag attatggttt agtaatcact gattgtaatg aatacaatga ggacttactt
    19921 ttattagaaa aaaagtggga agatttaaat attgaaatta aaaaagtaag aatgaaagaa
    19981 cagaataacc agttattaag tataagtgaa gagttttttt ctcttgcaaa tgatacagtg
    20041 tttaaaatag aaaacttttc taaagaaaaa agtaattact taatgacttt aataataatt
    20101 atatctatta taggtatatt agcatgtatc atattgattt tacaatatag taaaaaaatg
    20161 gttagactgg aaaaattaaa tgtagattta aaaaatattg catataaaga tgaactaaca
    20221 ggagtaaata ctatagaaaa atttaaattg gatgcaaatc aaaatatttg tatacataag
    20281 gataagaaat ttgctgtatt ctatattgat tttgaaaact ttaaatacat taatgatata
    20341 tttggatatg attatggtga catgatactg aaaagatatg ctaatcttat gatgaatgac
    20401 atagggaaat atgaaatatt tgcaagagaa attgcagata gatttgttgc gttaagatgc
    20461 tatgtagata aggaagattt agtggtaaga caacgagatg tagacaatga actaattaat
    20521 ataacaaatg aaattaaaaa taaacatagt attactgttg ttagtggaat ttgttgtatt
    20581 gaagatgtaa atgaaaaatt aagtatagat ggtttaataa atagagcaaa ttttgcacaa
    20641 aaaactgtta agaataaacc aggtactaat tatgcttttt ataatgacag tattcgtaaa
    20701 aaaatgattg aagaaaatac tatcaaaagt agaatacatg aagctattga aaaaagagaa
    20761 tttatagttt atttacagcc taaagttaat ttacatagtc aaaaaataaa ttgtgcagaa
    20821 gctttagttc gttggcttac tccggataaa ggtattatat ctccagcaat atttattcca
    20881 gtacttgaga agaatttttt tattgcctta gtagataagt atgtttttga ggaggtttgt
    20941 aaatggatta gaaagaggtt agatgaaaat aaaccttttg ttcagatatc agttaatgtt
    21001 tcaagaatac aattttataa cactaaattt gtagaaactt attccaatat acaaaataaa
    21061 tacaggatac ctaaaaatac tatagaaatt gaatttacag agagtgttgc ttttgagaat
    21121 caaaaacatc ttttggaaat tatacatgat ttgcatgaaa atggatttac ttgttcttta
    21181 gatgattttg gaaaaggtta ttcgtcttta agtgttttaa aagatttacc atttgatgtt
    21241 ttaaagcttg attctatgtt tttcaaggct agtttagata aagataaaga gaaaatagta
    21301 ataaaaaata ttgtacatat gttaaaagaa cttaatatca caactgttgc tgagggcatt
    21361 gaatgcaagg aacaagttga atttcttagg gatattgggt gtgatttagt tcagggattt
    21421 gtattttata aaccaatgcc aatattagaa tttgaaaaaa tattggataa agagtttgtt
    21481 tataatagct aaaatgtact ttataaaata tgaactaatt aagtaaaatt attttgtact
    21541 gaataaattt ttactttatt tacagaataa tgattattta cagaaaagcg cgaaaagtgt
    21601 attcctttaa gtgtgggatg gatagcacaa atagaggaga aaaaatattg gccatataag
    21661 aatgcataat aatataaata tttaaaaggt cagcattata taaaaattaa aaagaaaata
    21721 aataattttt ctataaaaaa gagactatga gaaaatttac tcatagtctc ctaaataatg
    21781 tatttatttt cttcctgcaa cccattttaa tccccaatga aattcagtat ccatttctct
    21841 atgacctttg aagtatttaa ctactttttc aacactgaat gtctcatcat ttacattttc
    21901 tatcaatgct attgaacaca tattatagcc atttttatgt tcatcaagtt tgacaactaa
    21961 gtctgaatct tgtttttgtt tgattgttac aattccatca gcttcagacc agttagctac
    22021 accttcgtat ataaaagcat aaataagtat tctttttata tcttttattt tattaccatt
    22081 tattcttaaa ttttctcctt gtgtattact tcctgttctg tcatctccat ctaattgagc
    22141 aaaaggtgga ttgtctaaac taccaaaggc atttcctaat gcttgaacag caccttttac
    22201 accatttttc atctcaaata ggcaacccaa atctaaatct atattactgc ttcttaaaga
    22261 gccaaaaaaa cccttttttt gtactttttg attccaattt aaattaacta aaatttcacc
    22321 taaatcgcta ttttcctttt ttgtaaggtt tattttttgt ccctttttta attctatagc
    22381 catagtaatc ttcctccaca aacatagatt tatttaaatg gtagcactta ataagtcaat
    22441 attcaacaag attagaataa taatattaaa attatactaa atatactaaa aagtaggaat
    22501 cacaatacct ttgtattttt cttcaattaa gcttcttgct ttatctgatg taagaacatc
    22561 ttgtagaact ttaatctttt cactgtcttt atcttcagtt ctaaatgcta aaagatttgc
    22621 atactcgtca tttataggag ctgtataaat tccgtttttc ttggcatcaa gaccagcagg
    22681 tacagcataa gtagtatcta taaatgcagc agtcacatct ggaagagaag atggaatttg
    22741 agcaatatct aactctttga attgtattcc ctttggattt tctattatgt ccttaggact
    22801 aactaatcca tcccctttag gtaacgttaa aagtccaata ctttcaaggt atctaaggtt
    22861 cttagaacaa gaagatgggt tattgcttat tgcaattgta tctccttttt taaattcatc
    22921 aacagatttt atcttataag agtaaagtat tgctgggcat acatatagtt ttttaccagc
    22981 cattattttg taacctttgg ccttaacagc ttcttttaga taaggttcat gttgaaatag
    23041 atttgcatca atttcaccat tatttaaagc ttcgtttgga agtatgtaat catcaaagtt
    23101 tttaacttct aaagtatatc ctttttcttt aataagtggt tttaattctt ctaataactc
    23161 tcctcctgga actaaagtag caccaacaac aattttttta tcatctttag ctttagaaca
    23221 tccaaccaca gctaaagtta gtactagaca taataacaaa cataataatt ttttgaattt
    23281 catataaatt ccctcctgaa tataattgat tagttaaaaa taaaatacca ttctttatca
    23341 cactgcaaaa taaaaataag cctgagttta aactcaggct ttaaaagcta atttagactc
    23401 atctttcagt ataatactgc aggatttagc accaatgtat ataaaaatat actggttgct
    23461 gggtttcata gggccagtcc cttcaccact ctggataaga agtatcaatt tttaattatg
    23521 ttataaatat tatcatgatt ttttacgtct gtaaacataa ttttataaat attttaaaat
    23581 ctttaatgta ttttatttaa cacctaaaat tccagaaact agaatatcag tatcagcacc
    23641 taagaaatag tcatttattt caatagaatc cagagtattt ctcaaagcat tttcttcatg
    23701 ccttatacca ataagtttat tttctacaaa tgcaacatca cattttccaa aaaaatctcc
    23761 aaaaaatttt atactagtta ttatgccttt atcaacattt aaattaaact caacatttcc
    23821 accagaatat tttaattcat ttgaaagagt atattttggt gaattaccaa aattccaatc
    23881 ccaagtttcg tattttgatt tagtaagctt ttctatttgt tttatatcat gctcactcaa
    23941 acaataatta gtattatcag tatttgtttt agaaagataa tccattatag catctttaaa
    24001 ttctaatatg tccatttttg aatgtaaatg ttcacttata ttggttacac gagattttac
    24061 agattttatt ccttttcctt caaattttat aggtttaacc tttaatgctt ttgagaggtc
    24121 atttatttca gatgaaaata aaagagtccc atgatgcatt actttgtttt tgtagttgta
    24181 ttgagcattt ccagaaaatt tcttgccatc tataagaagg tcatttcttc cagaaaattc
    24241 ggcattgaca tctagtgttt taagtaaatc tattatgggc tgagtgaatc ttctgaaatc
    24301 actaaaatta ttattgttac aagctataaa tgtaaaattt atatttccca aatcatgaaa
    24361 tacagctcca ccaccagact gtctcctaac gacaggtata gaatattctt ttatatattc
    24421 taaatttatt tcagaaagtg tgttttggtt ttttccaaca attatagaag gttcattcct
    24481 ccataaaata aataagtctt catcaaaatt ttttagaaga tattcttcca ttgctaaatt
    24541 aaaataaggg tttgtttttt cattataaat taataacatt aaagtagttc ctccatagaa
    24601 ttgataaaat ttttattatg aatacaatta attttataat tcgacaataa tataaaaact
    24661 cctttaaatt agaaatttta aaaactacct tgcaaagttt aaataaataa tatatactca
    24721 aaatatgttt aataaaagtg aatagaaggg tagaggcgca ataatctatc agtactaata
    24781 aggagttaag cacagagagt tattaggaaa ggagatattg ccgaagtttt ataatcgatg
    24841 ctttaagatt atattgctgg gggtacatag aaaatatgta caactgtcac aggtttattt
    24901 gtggtgagct atcatttaag gaattatatt atatttattt ttactattgt tgatatattg
    24961 taatgtttaa gccttgagtg tttaccactc aaggcttttt tagttttatt gtaacataat
    25021 accctgtaca gttcacaaaa ttaaaaggag agggagattt atatgaggaa caaaaaaatt
    25081 aatattattt ttctgacgac aatcatgttc ataatgtcaa cagtaatggt ttttgcagaa
    25141 gaagatatag acactattgc actagccaat gcagaaaaat ttgggatttt aactttaata
    25201 ccaccattag ttgctataat actcgcattt ataactaaaa acgtaataat ttctttactt
    25261 ataggtattc tttctggtag ttttataatt aaagctagtg gtattaatgt ttttgcgaca
    25321 tttatacagg catttttaga tttagtagat agagctttag tatctttagc agacccttgg
    25381 aatgcgggga taatactaca ggttttgtca attggaggag taataaatct tgttgctaaa
    25441 atgggaggag ctaaagctat agctgaagca cttgctaaaa gagcaaagtc agcaaaaggg
    25501 acacagctta taacatggtt tttggggctt ttagtattct ttgatgatta tgccaattca
    25561 ctaatagtag gtcctatgat gagacctgtt gcagataaaa tgaaaatatc gagagaaaag
    25621 ctagcattta taatagatgc cacagcagca ccagttgctg gtttagctat aatatctaca
    25681 tggataggtc ttgaggttgg tttaatacat gatgcttttg aaagtataag tatagatgta
    25741 gatgcatttg gtatattttt aaatacaata ccatttagat tttacaatat attaatatta
    25801 gctttcattg taatatcagc attattatta aaagaatttg gtccaatgag aaaagctgag
    25861 ataaaatcta gaagtagaaa aattagtata gaccttgatg aaggcgtaga agagttggat
    25921 gatttagctc caaaaaatgg agtaaaattg agtgtatgga atgctataat cccaatagga
    25981 acattgataa tagttgcatt agcatcattt tattatagtg gatatacatc tataatggga
    26041 ggagacgata aggctctgat acaactattt acaaattctc catattcttt tgaagcaata
    26101 aaagaagcat ttagtgcatc tgatgcatct agagcattat tccagtctgc acttgttgca
    26161 agtttagttg ctattataat ggctgtagta aaaaagattt ttacaatatc agaagcaatt
    26221 gatgtttgga tagatggaat gaaaagttta gtaatcactg gtgtaatatt aatacttgca
    26281 tggtcattaa gttcagtaat aaaagagtta ggtacagcta aatttttaat acacctacta
    26341 tcaggttcac taccaccatt cttattacca agtttaatat ttggtttagg agcaataata
    26401 tcttttgcta ctggaacagc ttatggaaca atgggtatac ttatgccact tgcaattcca
    26461 ttagcatact cattaaatcc tgatatgtct tatgtaatag taagtactag tgcagtttta
    26521 acaggtgcaa tatttggaga ccattgttct ccaatttcag atacaacaat actttcttca
    26581 atgggagctg gctgtaatca tatagaccat gttaacactc aaatgccata tgctatattt
    26641 acagcagtaa tcactattgt atttgggtat ataccagctg gacttggatt gccaatatat
    26701 atagtattac cagtggcaat agctgctata tttgtaggta ttcaaataat tggtaagaaa
    26761 gttgatgaag cggaaataga acttgtagaa taaagatata aataagtttc accttttatt
    26821 atcactattt tgttagtttt tagataattt taaaaagtta ttgaaaataa tggcgaaata
    26881 tagtaaaata aaagagtgat aagcggatgt ggcggaattg gcagacgcac tagacttagg
    26941 atctagcgcc attggcgtgg gggttcgact cccttcatcc gcactttaca gtacttaatt
    27001 gtactgaaaa aaactggggt tgctggcttt cggagtgggc tatcccgttt ttattttttt
    27061 gtatatataa tagattggca ctttagtata aggtgctttt tttataggca aaaacagtac
    27121 atttatttct gttttattat cattcatcta ttagaatata ctttagttaa gaatgtgtta
    27181 ataagcagtg ttcacattct tgataaaatg tggaataata tgtattttta ctaagacgga
    27241 aatgaaataa ttaaaccatt aaataatatt ataagatgat gaataagtag ataaatcaaa
    27301 ttttgtagga ggatattatg gaaaaatata tatggttgtt tccattactt tttatttttc
    27361 atgatatgga agagataata ggttttggaa tatggcttaa aaaaaataaa tcaatgcttg
    27421 ataagaagta tccatttatc agtaaagttt atgaaaatta tagtactgaa ggcatggcat
    27481 ttgctgtgtt tgaagaattt attttatgca ttattttctg tatacttaca gttataacag
    27541 agaatcagta tgtatatcta ctatggttag gtagttttat agcatatacc ttacatttgg
    27601 taattcatat agggcagtca atcataataa gaaaatatat accttcactt attaccagta
    27661 tcatatgctt accaataagc atttggtgta taagtaagag tatttatata gttgattgtg
    27721 aaatgagtac aacaattctg tacagtataa ttggcattat aattgtagca ttgaatttaa
    27781 agtttgcaca gtcgctgatt ggaaaattta caaagtggat gtcaaacata taaattttag
    27841 tttattggac agttcaagga atcagaaatt ggaattttat agtattaata ttttatggta
    27901 ttaatataat gtataaaatt ttatagcaat gtatttcata agtataaagt gactagaatt
    27961 ttaagaaata tacaaattct ataaaaacta aataatttca agtatatttt aaaaatctta
    28021 tttttcacaa gtttaaaatt gtaaacgatt tcatcaagtc tatgaatata cttttaacat
    28081 gttatagtaa atctcaatat aaatttacat ttggttcttt gtgtgttatt ttaataataa
    28141 attaagtgaa aatgttattt tacattaata tataatatct ttgcataaga gtgaataata
    28201 taaaagagaa ataattgata ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata
    28261 aatataatgt gttgacattt aattgaaata ttcatacaat aaaattgaaa ataaatattt
    28321 ccaaatgaga ttagcgagag agtcctttat acgaaaaggc gccgaagaag aaatttaaga
    28381 aatgccaatt tttagagaag ctttcaggca aaaatatcgc taattgatgg gactctggag
    28441 agacttaagt taagatacta taagtcaccg aagaagcaaa ataatattaa attattaaat
    28501 ctttcaggta tacaaacagg gatatatagg ctaggattat tttccatgct gatatatctt
    28561 ttttttatga aaaagtacag gaaataatat caaagttact taaatacagt aggtctaatt
    28621 ttctagggag ggttatatat gaatgaattg aaaagagtat ctttgtacaa tatacacaaa
    28681 gagcttggag caaaacttgt tgaatttgct ggttgggaaa tgcctttaga atatgagggt
    28741 ataaataaag agcatgagaa ggtcagaaaa agtgcaggta tatttgatgt atctcatatg
    28801 ggagaggtac agataaaggg agcagagtca gaaaaattta tacaaaacct agttacaaat
    28861 gatataagcg cattaaagat aaatgatatt atatatacac ctatgtgtta tgaaaatggt
    28921 ggagtagttg atgatttatt aatatataaa tttggtgaag aagattattt attagtaata
    28981 aatgcaggta atatagataa agatgtagca tggataataa aacaaagtga aggatacaat
    29041 gtagatataa aaaatatatc tagtgaagtg agtcaattag caattcaagg acctaaagca
    29101 gaagaaattc ttcaaaaaat cacagatata gacttaaact caataaaatt ttataagtca
    29161 attccatcaa caaaagtatg tggttgtcct tgtttagttt caagaacagg atatactggg
    29221 gaagatgggt ttgagatata ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt
    29281 ctaaaggttg gaggagaaga tatatgtcca gctggtctag gttgtaggga tactttgaga
    29341 tttgaagcag ctctacctct atatggacat gaaataaatg aacacatatc tccaattgaa
    29401 ggtggtttat ctatctttgt gaaaacaaat aaagaatcat ttattggcaa atcaatttta
    29461 tcaaaagaaa aagaaagtgg agctaaaaga aagttagtag gatttgaaat gcaaggtaaa
    29521 ggaatgccta gaaatggata tgatataagg attggtgata aaacagttgg atttgtaaca
    29581 acaggatgtg catctccaac gacaggaaaa attttaggta tgggaattat agattctgaa
    29641 tatgcaaaag taggaaatga aataggtata gcaatcagaa aaaaagttgt tcctgctgtg
    29701 atagttaaga aaccttttta taaaaaacaa tataaaaaag ataatattat attaaacaaa
    29761 gaaaataaat tttcatatat acctgctaca agtgaagata aatcaaaaat gttaaaagta
    29821 gttggattaa attcagttga tgaattattt tcagatatac cagaagaagt aaaattaaaa
    29881 agagatttaa atttggaaat tggaaaatct gaattagagg taagcaaaat agtaaaaaga
    29941 ttgtcagaag aaaatttaag tttagaagac ttgacttgtt ttcttggtgc tggtgcatat
    30001 gaccattata taccatcaat tataaaacat ataacttcaa gatctgaatt ttatacagca
    30061 tataccccat atcaagctga aataagtcag ggaactttac aagtagtttt tgaatttcaa
    30121 tctatgattg cagaaattac aggtatggaa attgcaaatg catcaatgta tgatggagca
    30181 actgctgcaa tagaagcatg tataatggca atgaatcaga ctagaaaaag taaaatagta
    30241 gtgtcaaaaa ctattcatcc tgaaactctg tcagtattaa gaacttactt acaatataaa
    30301 gattgtgaga tagtagaaat tgacttttgt aatgaatatg ggactacaga tatagaaaaa
    30361 ttaaaagctt ctgtagacaa agatacagca tgtgttctta tacaaactcc taatttcttt
    30421 ggaattattg aagaaatgga agaaatagaa aagataactc atgaaaataa ggctatgtta
    30481 attatgagtg tagacccaat atcattagga gttttaaaaa caccaggtga gataggtgca
    30541 gatattgttg ttggagaagc tcaatcactt ggaaaccctc ttaattttgg aggtccttat
    30601 gtaggatttt tagctagcaa atctaaatat actagaaaaa tgccaggaag aatagttggg
    30661 caaagtttag atgtagaagg taaaattgca tatgtattaa ctttacaaac tagagaacag
    30721 catgtaagaa gagaaaaagc aacttctaat atatgttcaa atcaagcttt aaatgctctt
    30781 gtagcttcaa tatatatggc tactatgggt aaagaaggat ttaaagaggt tggtatgcag
    30841 tctatgaaaa aagcacatta tacctacaat aaacttgtac aaactggaaa atataagcca
    30901 atatttaaag gtaagttttt caaagaattt gctgtacaag gaaatcttaa tatagaaact
    30961 ataaatgata aactcttaga agaaaatata ttaggaggtt ataatttaga atacaattat
    31021 ccagaattaa aaaattcaac tttgctttgt gtgactgaga aaagaagcaa agaggaaata
    31081 gataagttag ttggaataat ggagggatta taatgaaaga atacaatagt ttgttgatag
    31141 atatatctaa aaaaggtagg aaagcatact cacttccaaa gttagatata gatgatataa
    31201 aaatagaaaa tatgattgac caagatatgg caagacaaag tgagttgaat ttgccagaag
    31261 ttggtgaatt agaattagtt agacattata cattattatc aaataaaaac tttggagttg
    31321 atacaggttt ttatcctttg ggttcttgta ctatgaaata taatcctaaa ataaatgaag
    31381 atatggcagc acttccaaac tttactggaa tgcatccata ccaatcttcg gatacagctc
    31441 aaggttctct ttctttgatg tatgatttat caagaagact tgcagaaatt acaggtatgg
    31501 atgaagttac actacaacct tcagcaggtt ctcatggtga gtttacaggg cttatgatta
    31561 taaaagcata tcatgaaaat agaggtgacc ataagagaac caaagtgata attccagatt
    31621 ctgcacatgg aacaaatcca gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag
    31681 catctgataa aaatggagct gtagacataa atgcattaaa agaggttttg aatgatgaag
    31741 tagcagctct tatgcttact aatccaagta ctcttggttt atttgaaaaa aatataaaag
    31801 aaatagctac tcttgtacat gaagcaggag gacttttgta ctatgatggt gcaaacatga
    31861 acgctattat ggggataaca agacctggag atatggggtt tgatgtggtt catcttaatc
    31921 ttcataagac tttttcaact cctcatggag gtggaggtcc aggagctggt cctgttggag
    31981 ttaaaaaaga attagtacct tttttaccaa taccagtaat tgaaagaaaa gaagataagt
    32041 atgtactaaa ttatgataga gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt
    32101 ttggagttct tgtaagagct tacacatata tactaactat gggaagggat ggacttaaag
    32161 aggctagtga aatggcagtt ttaaatgcta attatataaa ggaaagtata aaagatgatt
    32221 atattttgcc aatagatacc ttgtgtaagc atgagtttgt attaggtgga ttgccaagag
    32281 gagaggccaa tataaaaact atcgatattg caaaaagact actagattat ggataccatc
    32341 ctccaacaat gtactttcct cttattataa atgaggcact tatgatagaa cctacagaaa
    32401 gtgagagtat agagacatta gatagtttta tagaagctat gaaaagtgtt gctaaagagg
    32461 caaaggaaga accagaacta ttgaaaactg ctccacataa tactttggtc aaaagagtag
    32521 atgatgcaag ggcagtaaag aaacctatac taacatggtc acaaagataa atagttaaaa
    32581 agtagagatt taaagtataa agagaattaa atttgtaaga aaaagctatc taacttaatc
    32641 tagtttagat agcttttttg tatattagta tgtatggtat tttctatata aagttttaaa
    32701 ttatattagt gcttatatag aatattgata tgatgattat gtatagatat taataagaat
    32761 tgttgaattt atcttgcctt aactttattt actcttttat attaagaaaa acttaactaa
    32821 aatatagcga aattcataaa aatttaggaa atcttaagaa ttataatatt taaatcttta
    32881 gatttatatt ttattatata aactatagaa taataaaata aaaaaattga gaattttata
    32941 atatatttgt agatttgagt ataaaaaagt gctaagttga aaggagtaaa atatggataa
    33001 ggttaattta gaaaggtata gttgtgatat aaatgttggt ttaaataaaa atcaacttca
    33061 aagtagaata gatgataatt taattaatgc atttgataat ggaaaaacca aaacatataa
    33121 acaaattttt aaagacaata tatttacact ttttaatctc ataaatattg ttttagtctg
    33181 tttattagtt tcagttggtt cttttaaaaa taccttattt atatttattg ctgtaattaa
    33241 tactattata ggagtaatac aagagataag agctaaaaga ttattggaca atctatctgt
    33301 tatagtttca aataaggtat ctgtcataag agaaaaagag aaaaaagaga ttgatgtaca
    33361 agaattagta ttagatgata ttatgatttt aaaaacaggt aatcaaattt gtgcagattc
    33421 aaaagtttta aatggaaaat tagaggtaaa tgagtctttg gttacaggtg aatctgatat
    33481 tattataaaa aataaaggcg attttttata ttctggaagt tttgtagttt ctggagaagc
    33541 ttttgtaaga gttgaaaata taggaaaaga taactatgca aataagatag ctaatgatgc
    33601 aaagatttct aaaaaacata attctcaact tagaaattca ttaaatgcaa tattaaaaat
    33661 tgtaggtata ataataattc ctttaggaat tatattgttt gcaaaacaac attttggaaa
    33721 tggagattct atagctacat ctgtagtagg tacagtggca gctgtaaatg gtatgatacc
    33781 agagggatta acattactta cgagcattgc tctagctgta ggaactataa aacttgctag
    33841 tcataaaact ttagtgcaag aactgtactg tgtagagact cttgctagag ttgatacact
    33901 atgtcttgat aagacaggta caattacaga aggtaaaatg caagttgatg atatagtaat
    33961 gttggaagaa gtggatatta atgaaataat ggggaatata tgtaattctc tcaaagatga
    34021 taatgcaaca ctaaatgcaa taagagataa gtataatgtt ttagatacgt ataaagctaa
    34081 gaatttaata ccattttctt cagaaagaaa atacagtggg gttacctttg aaaataaagg
    34141 tacatattta ttaggtgcat ttgaatttat atttaaggaa aaaaataaaa aacttaggat
    34201 agaagttgaa agatattcta aaaaaggaaa cagagtaata gttttagctc atagtgattc
    34261 ttatatggaa gataataata ttcctgaaga tataaagcca ctagctttta tattgatatt
    34321 ggataaaatt agagatgaag ctaaggaaac tttagaattt ttttacaatg aaggtgtaga
    34381 tataaaaatt atatctggtg acaatcctat aacagtaagt gaggtagcta gaaaagcagg
    34441 tcttaaaaca gctagtaatt ttattgatgc aacaacactt aaaaatgata gtgatatata
    34501 caaagcagtt gataaataca gtgtttttgg aagggtaagc ccacaacaga aaaaacaaat
    34561 gattttagca ttaaaaaaac aaaatcatac agtagctatg actggagatg gtgtaaatga
    34621 tgtgctagca cttaaagaag cggattgtag cattgccatg gcatctggaa gtgatgttac
    34681 taagaatgtg tctaatttag tcttattaga ttcaaacttt gcatcaatgc caaaggttgt
    34741 tatggaggga agaaaagtaa ttaataacat tcaaagagca tcttctttgt ttttagtaaa
    34801 gacaattttt tcattcttct tgtcgttatt aacattattc ttcttcagta gacagtatcc
    34861 atttgttcct atacaattat cactcattgg ggctgtaaca gtgggtatac catcattctt
    34921 cttagctctt gaagcaaaca aaagtagaat ttcaggaaat tttttactca atatattaaa
    34981 aaatgcttta ccaggagcac tatgtgtgac agtaaatgta attattgttt acaatatagt
    35041 tgaatattta ggttatagtt caagtgtatt ttccacaatg tgtgctatat taactggtgt
    35101 atgtggtctt ttaatattaa gacaacaatg tttgccattt aataaagaaa gattattttt
    35161 aatagtaagt atgactattg catttgttat tgggattcta tttttaggtg gattattctc
    35221 atttgtagca ttgagtagag aattaattct cataactatg gcattagcaa tattaatgcc
    35281 aattatgata aaagttatgt tatttgtctt agatgagata ttagagacaa ttgttccaga
    35341 tttaaattaa ttttaaaatg atataaataa gagcagtttt agttgaactg ctctttttag
    35401 atgtgtttaa ttttatgtaa gaatatgtat ttttattaat cagctatata tgagcaacag
    35461 tagtcaacaa cttcattaac ctttaagtca aaacttgaat cactaggaac attaaatttt
    35521 tgcccttctt tataagttac aaactcattg cttccaggca gttttacatc catacttcca
    35581 gcaagcatct ccattatttc ttcttctcta gtagaaaaag tgtattctcc tggcaacatt
    35641 aatcctaaag tttttctttc tccattaggc agtattatta ctctgctact taccttacca
    35701 tcaaaataaa cgtttgcctt ctttactgca gtaacattct taaattcact cattagataa
    35761 aacctccaaa attatttgta catatatatt atataaaaat aatgtaaaaa atgctatata
    35821 tttacaaaaa aatttagtat ttattaaaaa aattaataag aataaaaaag agatgaaatt
    35881 gcttaaattg ataaaaaata taaaataaat tgcaaagtaa gcaagaaaa
//
LOCUS       DAMJYX010000029        34881 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJYX010000029 DAMJYX010000000
VERSION     DAMJYX010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.029"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(106..366)
                     /locus_tag="PC479_001572"
     CDS             complement(106..366)
                     /locus_tag="PC479_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804120.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(629..982)
                     /locus_tag="PC479_001573"
     CDS             complement(629..982)
                     /locus_tag="PC479_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804121.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(999..6320)
                     /locus_tag="PC479_001574"
     CDS             complement(999..6320)
                     /locus_tag="PC479_001574"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804122.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(6337..7320)
                     /locus_tag="PC479_001575"
     CDS             complement(6337..7320)
                     /locus_tag="PC479_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2804123.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(7321..8019)
                     /locus_tag="PC479_001576"
     CDS             complement(7321..8019)
                     /locus_tag="PC479_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2804124.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8016..9068)
                     /locus_tag="PC479_001577"
     CDS             complement(8016..9068)
                     /locus_tag="PC479_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2804125.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9061..9489)
                     /locus_tag="PC479_001578"
     CDS             complement(9061..9489)
                     /locus_tag="PC479_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2804126.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9489..9815)
                     /locus_tag="PC479_001579"
     CDS             complement(9489..9815)
                     /locus_tag="PC479_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2804127.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9831..11360)
                     /locus_tag="PC479_001580"
     CDS             complement(9831..11360)
                     /locus_tag="PC479_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2804128.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11642..12064)
                     /locus_tag="PC479_001581"
     CDS             complement(11642..12064)
                     /locus_tag="PC479_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804129.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12064..14517)
                     /locus_tag="PC479_001582"
     CDS             complement(12064..14517)
                     /locus_tag="PC479_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804130.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(14517..14684)
                     /locus_tag="PC479_001583"
     CDS             complement(14517..14684)
                     /locus_tag="PC479_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804131.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14711..15157)
                     /locus_tag="PC479_001584"
     CDS             complement(14711..15157)
                     /locus_tag="PC479_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2804132.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15283..15711)
                     /locus_tag="PC479_001585"
     CDS             complement(15283..15711)
                     /locus_tag="PC479_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2804133.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15725..16789)
                     /locus_tag="PC479_001586"
     CDS             complement(15725..16789)
                     /locus_tag="PC479_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2804134.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16794..17237)
                     /locus_tag="PC479_001587"
     CDS             complement(16794..17237)
                     /locus_tag="PC479_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804135.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17486..17926)
                     /locus_tag="PC479_001588"
     CDS             complement(17486..17926)
                     /locus_tag="PC479_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804136.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18031..18228)
                     /locus_tag="PC479_001589"
     CDS             complement(18031..18228)
                     /locus_tag="PC479_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804137.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18397..18717
                     /locus_tag="PC479_001590"
     CDS             18397..18717
                     /locus_tag="PC479_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2804138.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18799..19305
                     /locus_tag="PC479_001591"
     CDS             18799..19305
                     /locus_tag="PC479_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804139.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            20006..20761
                     /locus_tag="PC479_001592"
     CDS             20006..20761
                     /locus_tag="PC479_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2804140.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20904..21332)
                     /locus_tag="PC479_001593"
     CDS             complement(20904..21332)
                     /locus_tag="PC479_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2804141.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21753..21953)
                     /locus_tag="PC479_001594"
     CDS             complement(21753..21953)
                     /locus_tag="PC479_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2804142.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22136..23305)
                     /locus_tag="PC479_001595"
     CDS             complement(22136..23305)
                     /locus_tag="PC479_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2804143.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23541..24362
                     /locus_tag="PC479_001596"
     CDS             23541..24362
                     /locus_tag="PC479_001596"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2804144.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(24438..25826)
                     /locus_tag="PC479_001597"
     CDS             complement(24438..25826)
                     /locus_tag="PC479_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2804145.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25961..26719)
                     /locus_tag="PC479_001598"
     CDS             complement(25961..26719)
                     /locus_tag="PC479_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2804146.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26721..27437)
                     /locus_tag="PC479_001599"
     CDS             complement(26721..27437)
                     /locus_tag="PC479_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2804147.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(27439..28146)
                     /locus_tag="PC479_001600"
     CDS             complement(27439..28146)
                     /locus_tag="PC479_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2804148.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(28262..28798)
                     /locus_tag="PC479_001601"
     CDS             complement(28262..28798)
                     /locus_tag="PC479_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2804149.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28900..29970)
                     /locus_tag="PC479_001602"
     CDS             complement(28900..29970)
                     /locus_tag="PC479_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2804150.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(29963..31021)
                     /locus_tag="PC479_001603"
     CDS             complement(29963..31021)
                     /locus_tag="PC479_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2804151.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(31008..31340)
                     /locus_tag="PC479_001604"
     CDS             complement(31008..31340)
                     /locus_tag="PC479_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2804152.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            31539..32660
                     /locus_tag="PC479_001605"
     CDS             31539..32660
                     /locus_tag="PC479_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2804153.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(32788..34686)
                     /locus_tag="PC479_001606"
     CDS             complement(32788..34686)
                     /locus_tag="PC479_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2804154.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        11184 a         5983 c         3698 g        14016 t
ORIGIN      
        1 attaatattt ttaaatatca aatccttctc aataaaatag ggtactatct acaatactac
       61 tttatctttt cctaacaata tataatctgg tgctactctt tcatgttata caccgttttg
      121 taacatacct tttataccag taacatcttc cttaatttcc tctacatctg ttttcattat
      181 acccatttga acaagtatat ttttgtttat ttcttgttgt tgtgtagata attctataaa
      241 attttctaca gtttttttat acatgtctct atcttctttt ttctcctgca tagtattttt
      301 aaataataga gcacatagta tacctattgc ccctagacta ctcaattcgg ttattaattt
      361 atccattata gcctcctatt gtctaaattt tttatactta tagctttaat ttttgttata
      421 ttcttacatc atgcaatatt atattttcag gattatcaat ttaatcttct gtactttaaa
      481 ttgtaggtgc aatttataga ttgaataaat aataaatata tatacaattt ctaatataac
      541 aaaaaataag tacaaggctt acttattaaa gttaagacct tgtactttac ataaaaaaaa
      601 taactaatac atagttattc taaatttact attcaactgg tatttctaat tctttgcatc
      661 taattctaac ttgctctcta attactgctg gtacttcttc tattgttttt ttgcctctta
      721 aaattaaagc tacatatact tctaccattc cactctcacc tccagatttt cttacactaa
      781 aagagcttat attacttgct tcattgacag aactttgatt taacaacatc tcatatattt
      841 ctgcatttgc tataaaatta tttgttatat catcatcttg ctttacttga cctatactta
      901 tgtctgaaat gtctttatac tgcatttcta cttttctacc ttgtttgatt tgttctattt
      961 tatttttatc gaatttaaac aattttcaca cctccatttt aagcttgtgg ctcgttgctt
     1021 tcagcatcat atctaaaaat catagatgga cgttttatag ctgtccattt atctcctttt
     1081 tctactatga ttttaggctg agcacttttt atgttggctt cttctaattc atagatttct
     1141 tggtaacttg agtctgattt tttgatttca actagtttca tgtctttgaa taagtcagat
     1201 ttatttattt ttacttgaac tttagtttta tttattaatt gatatatagg attgttttcc
     1261 atcatgtcat cctttaatcc ctcatttaat gttactatta aatcagtgtt attttcagta
     1321 atgtttatga ttgaatatac tttattgttt atcattaaaa tagaatcctt ttttacttta
     1381 agccctgttt ctgatttaaa tataaacata cttgaatata aatcgcctgt aggactactg
     1441 gctttaatca gtatcttatt ttctcctaat actattttat tttgtggtat ctcaaatata
     1501 atgctattta tttccaaatt agtcttagta gatattaatt catcatttat aaacatttca
     1561 agtttttcta taacattatt agggtcttgt actacaactc tcacttgaga tattgatttt
     1621 tcaactattt ctatagatgc tttacttgta tctacttttg ttatcatgta tattgtatca
     1681 tctgaaagct ctatatctaa aatatcattt agatgttcag gaattggtat tttagtaaat
     1741 tcatataaat cagtaattga tatagatatt ttatacatag atttattttt accagttatc
     1801 catagactat tatcattttt aattactagt atgtggtcat cattatgtga cttaatttct
     1861 ttaacattag ttaatacttt agtaaaagtt cttattatct tatctttttc actttctcct
     1921 agtccaaata atccatttgt attgtaccct tgagcgtaca actctccctc attagttaaa
     1981 acaagtgtca ttgagttata tatacttata tctttaacag aaggtaatcc agtaaccttt
     2041 tgtaactctc ttacatcatt attgtggcca actcccaatt ggtactcagt attctttcca
     2101 tatgaccata gagtattatc tgtcttaata atataggagt agttatctcc acaagcaact
     2161 tttttgacat ctgatatatt tacttcagta agagttaata attctatatc ttcaagggtt
     2221 tgattaattt ctttatatga attacttcct attccatata acttgccatc cttagcaaca
     2281 gccaatgaat gtgtttttga tatatcaata tcagatacct caaatggcaa tattattgaa
     2341 gtatacttaa aattatcttt aaatatgttt ttataattta tatacttatt atttgttttt
     2401 atatacttat cattaaacat tactagcttt tcatttttct tattaaaaac tattttatct
     2461 gatgtgttat ctgttttaaa atatgaatgt agggaacctt caagagcttc atttttatca
     2521 ttatcatatc tatggtgaat acctaaatga ttatatctat tatctcctgt tgaagaataa
     2581 aattttccat atatatcttt aataaatata gtattgtaac tagaaaatac agtatcaaca
     2641 tctttcttta tagatacttt ataatatgga actgacatat ccctataaga ttcatcagat
     2701 ataccataag tatctctacc tgatgatatc aaattgttat tattatctat ataaagtgta
     2761 ttattagctg ttacaaaaac atctttaaca tcttccacac tatttgtatt attattattt
     2821 gctatacttc catatccact aagacctcca acaaatagtt tattgtcatt tgttaaaatg
     2881 gctgaagtat atccattaac tactattttt tttgcattta taatattatt aattactcgt
     2941 atacccttta tatcattagt ttctgttgaa aagaataccc tctctttttt ccctgtagta
     3001 taaataacat tgtcatttgt tataaaaaat gtagcttcac ttcctgctgt tacaacagat
     3061 tttacattct caatatttat ttttgtaaat gtacttatac ttccttcagt aaacccaaaa
     3121 tatccattta caaatccaca tacataacat gtattatcat ttttaacaat tattgataca
     3181 tcattacata tatacacatc tctaactcca tttataggta tacgtgtaaa tatattccta
     3241 cttgtagtat cacccaatcc taactgtcct tttccattat tacctgctcc atataaaaga
     3301 ccatcattag ttaataatag agatgtgttt ccatatacag ctacatattt tacattttca
     3361 acatttattt tagtaaatac atttctattt acattatctc ctattcctaa ctgaccagaa
     3421 ttatttaaac caacagcata acaggtatta tcatttttta tcgcaaatac atgtttacta
     3481 ttgtcagtaa atctatttac atttatagat tttatattat caatatttat tttagtaaat
     3541 acctttctat ttatctcatc tcccactcct aattgaccta catcatttaa accaacactg
     3601 taaagcatat tattattagt taataaatat gtagacttcg aatggctata tatttcttgt
     3661 atattagaag atatttcact actgctattc tttttattgt aatttatctt attatttaaa
     3721 tttaaactac tattaactga tataccatgc gtacccttag agttatatcc acatataaat
     3781 aagctcttat cagttaatat tgcaaatcta gttttatctc tacttaaatc ataaggaggt
     3841 ataaatatat tttttatatt tttcattacc tttttataat tagtggcatt atatccatct
     3901 tgtgtatctg aaacatctag ttcttttgca ctttcagata tattcccaaa accatataac
     3961 gtgttagaag ataaatacaa tagagcttta tataaaaata tggctttatc agaggtaata
     4021 ggacctgaat ttatattaga tgctgcaata ttattagatg attgattgtt aagataattc
     4081 cttccatatt gatgtatata agatgaacct ccagaaataa gtaaattatc tggatttaca
     4141 ttttttaaat tacttgttga ttctccataa tacaaatagg caaaatttgt agaatatgat
     4201 gattgtgtac accttgtggg ataatagtta tatatgtaat tactaatata tccagcatgt
     4261 gtaattcctt gttgttgaac tctataagta gaatcacttg gctttgtcca taaagtacca
     4321 tggtatccag ttaaaaaaga accataattt gtacataata tagccatata ttcatatata
     4381 ataatattat taattttagt aatatcattt gtacaagaat ttgtactata acttgctcct
     4441 gaaggattta cataaaacca gttatttatt aatgttaaat ttatattgac tccatcatta
     4501 ggattaacac ccaataaata gtttgtattt ataccagttg catatacctt tttttcgtca
     4561 gccacaataa atgttgcatt atatgaacta tatgcatctg ttacattgct aacaccaatc
     4621 tcagtatatt gacttgctga tttattacta agtccattaa cataacttga actctttcca
     4681 gaagtatata tttgcttatt attagttaca attaatgaaa accagtttga taaatcagta
     4741 tttataacaa ctttttttac attatctata gatattttct taaatatatc tgaattagaa
     4801 ggaagaacac ctgctattga gttactattt ggcccagaat aataacaagt gtcatcattt
     4861 ttaatgtaga atattcctcc agaaatattt tttgatgcta ctatagactt tacattagag
     4921 atacttaatt ttgtcaactc atttaattga cttggtaagt cagaacccat tcctttgtat
     4981 ctattatttc ctagaccata tacatctcca ttccctaaaa taaccagagt acaataagga
     5041 ctaaaaacga catctcttac agaatgtata tctagtggaa gttcaatatc ctttatttcc
     5101 atttcttctt ttaataaagt taatttatcc tcagatttaa ttaaatttat ataattacta
     5161 taactatatt tttcactatc cattttctta aattctacaa tgtgcatatc atcaaaaaac
     5221 gaacctaatt gtccataagt attatctcct gtacaatatg aatcattatt tgaatcaatt
     5281 agtatgctat gtgataaacc aatgaaaata ttcttagcta caatattttt taaatctata
     5341 aatgaattat ctatattctc agagtaattt gctataccta actgatatat atcattattt
     5401 ccagcaacct tcaaaatacc tttattatct ataagcatag tattaccatt accacatgca
     5461 atagttttta tatttacttc ttctatctta gtaaatagtg atgattgaag tgcatttcca
     5521 aaacattttt cctttccaca accatatata tcattattat acgtcattaa cacagtgtgt
     5581 tcagaaccac aagctatagt cttaacatca ttaatagaaa ctaagctaaa taaatctcta
     5641 ttattcttat ctcctaaacc tagttgacct tgattattaa aaccagtaga ataacattta
     5701 ttgtcattct ttattataaa agctgattta ttatatgaga atacagcctt tacattatca
     5761 acatctactt ttataaatgt atttacattt gcagatgctc cattagttcc tagacctaat
     5821 tgaccataca aattatttcc agcaacatat aaagttccat cattgtataa aagatatgtt
     5881 gattgaagtc cacatgcaat atcctttacg ccttttattt ctggaacctt ctcaaatgtg
     5941 gaaactgttt ttcctacttt ttctaatccc aatcccattt gaccatctgt atttatacct
     6001 gcgacaagta attctccatc tgaagtaagt ataactgcat gagattctcc acatgcaact
     6061 tttttaatat tagtagatat atttggtatt ttcgtaaata attttcttgt tgtattgtct
     6121 tctcccaatc caagttgaaa gttactatta tctccacatc catacaagat attattattt
     6181 tttagaccaa atgtatagtt acaacatcca gtaaaaaaat taataaagtc ataattatca
     6241 tacccttcat caaattcatc aactaccata tttttatcgc caaagaaatt acctctttga
     6301 agtagtttag ttcttttcat tataaaaacc tcctaattat cttatttcat acgaatattt
     6361 atttaaagtt atatctgtct ggccaattga atatgcccta ttagattttg tttcaacaaa
     6421 gttattatcc atttcatgta tatatctaac tctatttgca ttaaatggta gaaccacatt
     6481 cttaaatgta acactaccaa gttctccagt tatacacttt ctatttaaat ctaacttaag
     6541 gccttctata gctattatgt ttttagcatt agttaaggta tcataccaat atccagcttc
     6601 atctacatct atttctgact ctaattctaa gtatattaaa atatctaata ctttttcaga
     6661 taatttattg taactatttt ttaatacaga tagttcagtt tgtatttgac ctcctattac
     6721 atcatcaata gatatatttt taattttttc tactacctca tttatagctc ctgataagtt
     6781 tttagcttct gtagataaga gttcttctcc aatttttact tttatttctt ttatattttt
     6841 atcaacctta tcatctaaat cttgaatatc cttttgagtt acaaaaacca acgtcgggtc
     6901 tacttctaat gtaacatttg aagtattgga caattgtaaa atcattttta caaccaaatc
     6961 tttaatactg ccatcttcag cacgtggctt atacgtctct ggatacttac ctatagctat
     7021 tatattattc tcattatcca gaacagcagc ttctcttatc ataaatccac caactgttcc
     7081 tggtaaaata gtctctataa ctatccaatt aggatttttt tcatcaattc ttagagaatt
     7141 tatatttcct tcccaaacta cattctttaa agcagtttgt tcctctgttg gctcataata
     7201 ttctccatta ctatctccaa cttgaaactt ggttaatcca acaagctctc ctagtgcagt
     7261 tgcatttgct attttttctc taccaatttt ggttaatatt atataaaatt tttcttccaa
     7321 ttataccacc tccgatttag gataaactac tacattttct acgcctgtat catttgcaat
     7381 agctatatta atcttacaat tattttcaat gcatttaatg cttaaaggat aaacctttat
     7441 ttcttctcta cttaacattc cagctccaca gtttacaaca gcgctacatt ttataattgg
     7501 ttcaaagtat ggatgaactt ttacttcttc actacatacc atacctccac cacaatagac
     7561 catactttta tctagtatta ttggttctaa atagtgtaat aagtgagcag gttttactct
     7621 ttcaatcata tcgctacaat ctaaaagagt tttatagtca caattatttg ctataaaact
     7681 caatacgaat gtaaattcat cactataaac ttttatatct gtttctgatt ttgtatatgc
     7741 ctcacatata ctttttataa cttcaatagt actagtacct ctgcttctca ttttagcttt
     7801 tatattgcta cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga gtatgtcttc
     7861 ccacatatct aatccccatg ttgcagtgtc aacaaataat tggttaaagg tatcatcaat
     7921 atcttcttta agaatattta attctgtatc gtatgcacct tgtatctcct ctacaatgta
     7981 atttctatca aatgatggta gtttatcaat tagcttcatt gattttccac ctcactaaaa
     8041 ttaacagttg ttacactagg tattttatct tcattaatag ttatattatc tgtacttcca
     8101 tttataagta ggttacttaa atcgtgtaca ccagtagtat ttataagtat tcccattact
     8161 tttgtataga ttatctctcc tctaatatct ctaaagtatg tatttatact ttctaggaaa
     8221 gattctttta cattgtctaa tgtatatcca tcttctaatt tcattactgc acttatattt
     8281 atttctattg gtaatggtgt cacaactgtt atagttggtc caataggctt ctcttcatct
     8341 atatgttgct gacacctttc aattgtttct gtatcaacag cttgattatt tttcccaaat
     8401 atcaagacct taactgttcc cggaccatcc catcttggat aaaccttaac attatagact
     8461 ccatctactt ccaaagccca ttcttcatag tgagctttat ttccacttgt agcttgattt
     8521 ctttggattt tataaaatct ttcttttagt tcttcatctg tctctatatc tgtaccacct
     8581 tcaaaacttc ttgtgttagt aatctttgtt actccagata tattatcaac tagtttaaat
     8641 tcacaatttg taggtaaatt atatttctta ccaacttcca gagcttgaac tgggctattg
     8701 tcaccttctt cactaccaat agttacatct tttattacta caaatagtaa atctctatat
     8761 gatattattg tgccattatt tattacagtt cctttctctc caataaattc aacttctcca
     8821 tttgactctg tacctaactt tctatataca ccaaattcat tgactctttt atcgagaaac
     8881 tggttatatg tgtcttgtat aaaagccatt ttatgtatct ttgaaagttc tagatatatc
     8941 tttgaaagtt ctagattatt tccagatacc atgttgttta gaaaagaacc ttctccttta
     9001 taaatatcaa gattaatatt ttcaagagtt ctatttttta ttacttcata tgtctggtca
     9061 ctatacattt atttccacct ccccatagat agtggatact tttacatttg cactcaatac
     9121 gtctctgtta aagttagcac tttttacgtt tacttctaat atatatggat ttattagaag
     9181 ggcctctttt atgaatctac tagcttcact ttctgtaaga ccttttgtat atttttgtcc
     9241 tattagttct gaaagctctg tcccatattc taaagagtat atctcatgtt catatctatt
     9301 tgtctttata cacttgtata tccaaacttt taaggcttct tttttttcaa ttattttaaa
     9361 gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc catgccactt cacgaaagat
     9421 tggcaattct tctgttttag gtaagatata atcctctggg acacctataa aaggaaatat
     9481 tgtactcatt ataagctcac caacttactt acaacagcaa atttttcgcc tattctaaac
     9541 attattacca tatccccaga ttcaaaatta tctataaaag gattttttac ttcatgctgg
     9601 tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat ccaactatct
     9661 atcaaaatat cttctttttc taatatgatg ttatttgtct ctatttttaa atctggtaat
     9721 ttacttttaa tttttccaat aaaaaaagaa ggttgattat gatactttcc ttcttctctt
     9781 attattccta taaattcatt tattggatta gccattatat caccatcctt ttataatact
     9841 ctcctagctg tattaaaatc tcttctacta cttaacttac ttattttaac tacatcacct
     9901 ttttgtggag catgtaaaaa ttctccatta cctatatata atccaacatg gctgactgga
     9961 ttatgaaaaa atactaaatc tcctgcttga agattttttt gttctacttt cttgcctttt
    10021 ttagattgtt gatttgacgt tcttggcaaa ctaacattaa cttttttaaa acaatagacc
    10081 attaaaccag aacagtcaaa actacttggt ccatttccac cccatttata aggtttacct
    10141 agatgttttt ttgcttctga tattactatt ttagctttat cagatacatt accagaataa
    10201 ccaccaataa taatttttcc ttttcttctt ccaaatttac tagcttcttc aacatttcca
    10261 aataaaatat ctatatggta tgttccattc ttttctatag ttattgcagg tcctacgtca
    10321 tttactttat agactccatc acgaccagaa actcctgtaa ttacctgtat ttcatctcca
    10381 tagtttaata aaggatgttt atttaaaaac tcttttgtat aataagtttg ttcatattta
    10441 ccaaccatag gagcagcgca agttttttta gatgggtcaa gttttttctt tctacaatct
    10501 gtatctccac cttcttcttt tctaggacag taagctgtaa attctgctgt aaactctttt
    10561 cctcctgcat aatcttctcc ccctaagtta ctttcttcct taggttcatc ctgtcctgct
    10621 gacttttcat ccataagatt ttgaaaatta agttcaagct caatttgata ttctccattt
    10681 tgccaagtat gtttgtctgt atctatataa aatagtccta caagctttgt ataagaatct
    10741 ttaactttta ctcctctacc agttatacaa cttacatctc cataaccttt aagagaacag
    10801 cttttttcta tcccattaaa ctcgctatca atatctacat cttgattttc ttgttgctga
    10861 attacttttt gcattattac atttacttcc ttaaaaattt cattatctat tttttcgcta
    10921 atcttgcttc catactggtc taccactatt actttatttt ttacattttc catgctctcc
    10981 gaaaaggtgg tatttataat attaaatccc tcttcaaaca taacatttaa tgtaacagtt
    11041 cctttttcaa taacattaaa cttatctaaa ttggcctcta tcatatactt tttctttgtc
    11101 tttttacttg cttctgtata tgcactcatt attgtgtcat aaccatttac tcctataaac
    11161 atctttgtat acttgacatt ggtctttgct attgttccaa ctgaaagcct attttcagca
    11221 aatacttgct ttgctatgtc ttcaactaac ttatctttaa aattgtatga cacttcactt
    11281 tgtgtaagta aaaatcccat atctttagat acaaaactaa tttcattact gcttgaatct
    11341 ttagacctat taattatcat tcctctaaag agttctttct catctacata gaaacagact
    11401 gtactagcta ttggtatatc tatttgttgg aaatttacat caaatgatga ttgtattatt
    11461 gaaaactcta gtgtccttga tggagattta taatcacctg accatgatac cttgtctact
    11521 atgtcagtta tgtcatatat acttccgttt tttatgtgta cccaaatctt aataataaaa
    11581 aatcacctgc cttttcattt aattctataa gcagttattt ataacttatt atctataata
    11641 gttattgttt tggagttact tttggtatct ttattctttt atactctttt aaatctaatg
    11701 taaaatatac atctcgagtc ccatctcttt cttcataact aaacccttca attatgactt
    11761 ccatatttat atttgtttcc gtaattgtaa atcttaatat aaaaccttcc ttcatccatt
    11821 tttctatctt atttacacaa tcatatggtg atggaaaacc tgtatagtca cagaaagtat
    11881 agtctttatt tggaaaaaaa cttgatattg aaatgctttt aagagctaca ccaccaaaag
    11941 ttgctatttc acctattttg agtatactag aagagtttac agcagctttt ccattaatac
    12001 taaaggaaga tggaaatact ggaaatctaa atctatcttc agcttgtcta agccacattt
    12061 ccattataag acacccccta actgtgccaa ttggatactt gcaactaatt ttgatgttat
    12121 cttttctata tcagcttctt ctctaatcac tattgtatca gctaacttag ctatgtttat
    12181 atttccacca ttttttctgc ttctatattg atttgcttct tgtttagtta aaaccatttc
    12241 accttcatgg agtctggttt gatagtcatt ataaggaaca tagttcaatc caaatgcatt
    12301 attacctttc ttacctgaat aagctttacc agaatcacca gcctttgttc cagcatatgg
    12361 attatctttg taattttttt tactcttttc cactagattt aaaactgttc ctgtgatgct
    12421 tccatttttt atcttattaa tcatagtttg ccaccacttg catatatctt taactatttt
    12481 acatacttta tctaatatgt taaaaaaagc tcccaatatt ggctctataa ttttccatcc
    12541 agtttccaac agaggtccta aggtctccca tacagttttc caaataacac ccagtgtctc
    12601 tataaaattg tttatttgag gtgcatattc acctaaaaaa gtaaaaattc ttgtgataat
    12661 aggactaaca gcttcaacta caggtccgat tagttggaat gctgtagtta ctattgtacc
    12721 aaatatactt attaatcctt gtgcaattgg tgatttattg tttatactat ctagtaaatt
    12781 atttactaag tttactactg gaagtaatga atctattaat gctgtaaaat cgatattttg
    12841 taatagtgta cttcctgcgt taacaattgc ttttggtgca ttccctatca ttgttgctgc
    12901 atgattttca aaacgttctt gcttattttc aaccatcatt ttacgctttt cagcctcatt
    12961 agaaattcga tattgagtag aatcagaaaa ctcttttcca ttttttacac ttctagattg
    13021 catctggtca tatttgttgc caataattat cttaccattc attacattgc ctattttacc
    13081 agccattttt tcagataagc cagctaaatt gcctaatcca ctttctataa aagtttttat
    13141 tttctttagt ccttgaccta aaatatcatc aaaaggttta acaatactct tcaagccatt
    13201 gttaatgctt gtttgagctc ctgatattat acctggtatt gtattactca tttctttagc
    13261 taaccccatc tgctttacac ttatgccttc caatgcttca tcaaaagttt tataattttc
    13321 tcctaccaga gtattaagag attccatgtt tccttttgct gcacttaaga aagctgctga
    13381 tacatcttgt tcactacttg tattcatatt taaagctcta acatctacca tagcttgagt
    13441 tatatctttt tcttttttcc caccagtaat attactaatc attttagctt tagttcccat
    13501 tgcaacaaca tcttcagggt caaaaggcgt atcatttgcc attcttacta aactgccata
    13561 ataatcatca acatctattt gattaacttt tccagtattt cttttcatta cactttctag
    13621 aacaactttc tgattttgtt cctgaccaag taaatcaaca ccctttttca cagcgacacc
    13681 tatagtagca ccatttgtca aatcagatat cttccctttt atcgaaccaa gtattccact
    13741 tgccatatcc tttacagcta ttgcaccact ccatacctta cctgcaaata atctcaattt
    13801 gggaaatact ttacttataa tgccacttgc catatctttt atagctatta caccactcca
    13861 aaaagtattt ttaaacaaca ttaacttagg caaaattcta cccatagcac tactcaccat
    13921 atctttaaga ttaatacttc cttgataagg acgtttctct acttgtttta attttcccaa
    13981 gatactactt ataactctct tagtcatatc ttttgctttt attacgatat tccaaacctt
    14041 gccaccaatc ttttttaaat ttccaagtaa tttattttta gttttttcta cttcatcact
    14101 tttagcttta aatattaata taatcataat atttgaatta aactcatttt tcttttgaat
    14161 caacttatta aaataatcaa taatcatagc tatagttatt gctagtatca taatacttcc
    14221 atttaaactt ccaataactc ctgaaacagc atttatgcta gaacttaatt ttgtcatagt
    14281 tgaacttaca ctactagcag tagtttgaac ttttcttaac gaggaattta aactgtttac
    14341 tctattactt gcacttccaa tagaattttt tatgctacta gccattacat tagtggaaag
    14401 tgaaacagcc acagttgctc tcctaaaatt atcagttact gatataattt gattcatttt
    14461 tggtgtgtaa ttatctgtaa ggtacatatt tataccttct tctctcacat ttcccattac
    14521 ataccacctc ctgtcaaagg acacactcca tatccatgtt tcattttttc aacttcttcc
    14581 tcaatttcta aggcgaaaaa agcttcaata agctttaatt cgcctttatc cattgcataa
    14641 aaaagggacg gtcttatacc ttttttcttc caataatagt acattattgt agtcctggta
    14701 tccgtcccta ttagtttttt acctcttcta tcactgcatt tttaccataa cccataacat
    14761 cttgaagtat tctatataag ttggtaattt cacctggtaa taataatttt cttgctaatt
    14821 cttttggtgt tggagcctta aatttatcca ttagctcctt atttttaaat aaaagctttc
    14881 catccaaatc acaaacacct tctataattg taagcatctg catctccggt aagtctatat
    14941 ccgttttcat attttcatca attttaagac aagtattttg tatttcttca tctttactta
    15001 ttgttaaagc tctacacatt actttaaatt cctgcccaaa tatagttgat aatctcttaa
    15061 gttcaactat agtactcggt ctttctaagt tttctgtatc tgaatttaaa agtaaatcta
    15121 ctatgtttat attttttgat aatccatttt cactcattat tatttgctcc tttatcaatc
    15181 aaattcaatc aactaacttt tttacttatt aactatctga atttattaag tatatattta
    15241 ctaaatatca gtataaaacc aaatataaaa ctaaatataa aactaaatta tatcaagaaa
    15301 atcatactca gtaaatgtga aaggtgcttc tatctctcct tttacgccaa cctcccaatc
    15361 agctaaagtt aaatcatcaa atgctatatt ctttactgct attctttctg ctccataaga
    15421 atctgggtca tttaatttag atatagcaac aaacctaggt tcagaacctc tctttatctt
    15481 ttctccaatt aacttgtgca ttcttgaact aacgtggtat agagttattg agccttttcc
    15541 tttatatcct atatactttg tatcagtacc catttgacct gctattataa tatcttcttt
    15601 tgtaaattcc atctttgctt gaaatttctt tacttctgct actttgtttc catcaagcca
    15661 aagttctccc caagtaccac tcattacatt tctagcttcc atatttgcca tattaataat
    15721 cctcctatat ttctattgat aaatctatat cttccatagc atcaagtact tttatttttg
    15781 cttttaaaaa tactttagaa cctgtgttag cttcttttat ttcttgtaat gtcatataag
    15841 ataaatctac tccttttgat tttaaatacg atttttgtgc ttcaaaatct atttcaacag
    15901 tagaatcaga ttctataagt gctgattttt ctaattcttc taaataactt tttatagcta
    15961 ctatcaataa acatttgttg tcataactgt tagtaacctt tcctatatag tcatctatta
    16021 tcacctttct tatgtcacta tgtataatat ctaaagtgtc aactattttt attttctgga
    16081 acatttctcc tttttctgct gttaattcag ttaaagaatt tactcctcta gcaattctta
    16141 tagctcctgc ttctttaata agtataagct ctcctttatt aacctttgat tctgcatcaa
    16201 ctttcgtcat cttaggtata tcgactacat cactaagttt agtataagtt actgattgac
    16261 ttaaaggtgt acctgctata agtccagcca ccctacttgt aaactcatca acactgtatt
    16321 tcttttctcc aactaacaca tcttctgtag taaaattaat tatcccttca tgatttccta
    16381 caacttttcc taatacagct ttaaccttaa ccttatctat atctctaagt ttaattatcc
    16441 aattttttat agcagtctta tcagcttcta ctgcttttgg catacataga taattaaatt
    16501 ccttagtctc taaaaaatct aatgcagttt gaatatctgc ttctccttct attacataaa
    16561 ctaataactt atttggagtg ttaacatttc ccatcaaagc taaatttata tattctttat
    16621 tttcagcaga taaatcaact ggtatatcct ctttttcatg tatttcatta agacctagtg
    16681 ccttagcatc tttaagtacc attgcaatta ttcctctagc tgaacgttct ttaacagttg
    16741 tagctagctc cttaaatgat atgttgatac ttggtaatcc tatagccata ctatcactct
    16801 cctaaattta aattgatttc ttccatattt tcatatacta catcttcttc atatacacta
    16861 tccagatagt ttagtgtaaa tgtaaagttc aatacataaa ttgactcttc ttttttaata
    16921 ctttgttcta gctttgttat atttaatttt ctgtctgtta cctttatatt tctgttgaat
    16981 aacttattta attcatctga aacttcatac attctaatac tctttgactg tggttttggt
    17041 aagtatttta tagagataac aatatctttg ttattcgtat ttaacattac aggtgtacaa
    17101 ataactgata gtatacttac aaaaaaacaa gacccttcag agccttgcac actttcatca
    17161 tcaataaaaa tactttcagt aaagtttttt ttgagaatct caataattgt ttctaatatt
    17221 tccttgtatt taagcaaata atcaccacac tatcactaaa tattcttgct taatctgata
    17281 atcttactat aaatgatata agtcattttt aaatatatat ctcatttatt cctgtcaagt
    17341 atcttctata tttttaggta atattattct tttcaatcta tcacctcctt caatatggat
    17401 ttctttcaca ataacattat ctcacatatt ttattgccta ttgttgtaat ggtgtatata
    17461 aagtgttgta aaattgtcct attttttata atatctcact caatttaaat atcatctttt
    17521 tctttaaatt gtagcaagat gttctatcta agtgtagttt catagaaata tatgtcatat
    17581 tgttttttgt cttactatta taaaaaagat taaaaaattc catttctata tcatttagac
    17641 acgttagagc attttctatc ttctcttttt ctatttctat atcttctttt tccattttta
    17701 attttcttaa tctttcctct ctttttaaca cctcattttc cacagaagag cttatgttat
    17761 atgtgttact agtcttttct gtatatacca ttgctccaca tccagagtat tcattctcac
    17821 atctttttat gtccaagtta atattcttta tctttatatc caaatactta taattatgta
    17881 atcttttatc tgtttcttta aataatatat cttttttgtt ttccatagta cttccccttt
    17941 tttatatact tattattagc ttcaaattta caataatctc tttagtgcac agttttttac
    18001 taatctcaca accttcaaga acatattgtt ctataactgt ataaaaaaaa cttcttctat
    18061 attttgttct aaaatactag aaatcttctt agccataaga agtccaggat tttttatgcc
    18121 tctttctatt tgagatatat aatcatttgt aattccagcc tttctggcaa gttgaatttg
    18181 tgtcatacct ttgcattttc tataatattt caagttattt ttaaacattg ttttatacct
    18241 ccattactat taagaatatt ttgttcttcc tcacttagat tatatagaat tatttgttct
    18301 ttatcaatag attttagaaa aaaatattct tttatatttt attagtttta ttttttagaa
    18361 caatttgtta taatctatac ataaggtggt gttataatgt ttgccaaaag attaagagaa
    18421 cttagaaagg aatttggatt gactcaaaga gaacttggag aaaaagtagg cgtttcccaa
    18481 agagtcttag ggtactatga aacagaaaac agatttcctg atgaacacat tttaaataaa
    18541 ttggctgatg tatttaatgt atcagttgat taccttcttg gaagaacctt agttaaggaa
    18601 aatattgata cagtagctgc acatagaaaa aatccacatg aagagttacc tgaagaagca
    18661 caagaacaac ttaatgatta tatagaattt ttactaaata aatataaaaa aaaataaacc
    18721 atatgatgag cagtctattt ttctgctctt ttaatataat tcaaaggcaa acatacattc
    18781 tattttaggg ggatttttat gaataatttg gataagcttt ttgaattagc ttctcaagaa
    18841 gagataataa ttcattatac cacttatatt gcaggtgatt tagaaggact atatataaac
    18901 aaacatggta ttaaaattat atcattactc agtaatttaa aacaaaactc aaaaaaattg
    18961 acatccatct tagctgaaga acttggacat catttcacta gccttggata ctatgtatcc
    19021 tcttataacg attattatac aaaaattatt atagataagt gcgaaaataa agccttaaaa
    19081 tgggcttgtg agttcttaat tactgaagaa gatataataa acataattaa ctctggcatc
    19141 acttgcgttt atgaaatggc agacatactt aatgtggata ttactttctt tcaaaaaaga
    19201 ttagaatttc tatcattaaa aaaacagtct ttacaacttg gaaataataa atatttaata
    19261 ttaactaatt tgccgtattt ctacatattt gatccaattt cataataaaa atagtagtaa
    19321 ttttaactgg cttaacagta aaatctatat atttatttta acaaattttg gtttctataa
    19381 attcaatatt aatatataat aatgatatca tattatttaa aattctaaaa acaaatatat
    19441 gcttttgtta agaaaggttg tttgatgaat gtataaagaa aagttgtttg ataattattt
    19501 taagttctta gcactattat tttggccaat tatgtggtat aagtggatag taatttcaaa
    19561 tggtactctt gaaaatatgt tatttaccat atatgcaatc gtcgcaattg ttttcatcat
    19621 tttatattcg gtgtttatga taaaatataa agacattaca caaatagatt tcttttacag
    19681 gataagtact ctacttgctt ttatatttac actttttagc tttttaatat acccaaagag
    19741 tttatttttc ttgtatttaa agattatttt tactggaata tacctttatt attcaatagt
    19801 aaaaactctt aaatttaaag atgacgaagg tgtagtgggt attatgagct cacttttatt
    19861 aattgtaata actttgttct actaaattta attctatttg acttaatatg gctttaaata
    19921 ttagacatct acattatttt ctgatattat ttaagatggt agtcttatat taataatgaa
    19981 atacactatg aaggagttaa ctattatgga aaaaaaagtt ttagcaacag ttggagaaaa
    20041 agagataact aatattgata ttgaaaatgc attaaaatca ttagaccctt atcaagctat
    20101 gcaatttaaa actgaagaag gaaaaaaaca cttattaaat gatttagtga atcaagaatt
    20161 attcttctta gatgctaaag aagaaaaatt agatgaagaa gaaatattta aattagaaat
    20221 gaaaaagata gaagaaaatg tattaaagca atttgctata aataaagttt tatctagtgt
    20281 taatgtaaca gaagatgaaa aagtaaaatt ctttgaagct aataagagct ctttctctaa
    20341 accagaaagt gcaactgcta aacatatatt agtagatagt gatgaaaaag ctaaagaaat
    20401 acttgctcaa ataaaatcag aagaaatatc ttttgaagat gcagctctta aacattcttc
    20461 ttgcccatct aaagatatgg gtggtgattt aggaacattc ggtagaggac aaatggttcc
    20521 tgaatttgaa gaagctgtgt tctctatggc taaaggtgaa gtaagtgaac cagttaaaac
    20581 tcaatttgga taccatataa taaaattaga agaccttcaa gaaagtacag agtctacatt
    20641 tgatgaagtt aaggctgaag ttgaaaaaag tttattatat caaaaacaaa atgaagttta
    20701 tggaaataag ataaatgctc ttaatgctaa gtacggaaac cttgtaaaat acaacgatta
    20761 atcataaggt catatataaa aaagctgtct tttcacaata tttattagaa aagacagctt
    20821 tttatattaa agtttaaata ttcagtttca tattatcatt tttttattgt aaagaactca
    20881 gcttaaaagt ttaaataaga tttttaatta tcattagcta aatttttata tccacgtatt
    20941 atctcataac agtaacctat tacatctctt ctttttatta tcccaataaa tgtgtcttgg
    21001 tcatctataa caggaatgaa attttgattt aaagatttta taactaaatc ttcaatatct
    21061 gcgttgatgg aaacaggaga attatccatt tttctattta tatctgtcac tggaacatct
    21121 tccaatccct ttaagtccag atttaaatca ttttttaatg tccataaaaa atcaccttct
    21181 gttatagttc caacatattt accatcctta cttattattg gaattgcaga gtatttgtga
    21241 tattccattt tttctagggc ttgccttata gtgtagtctt catatatata tgctacttca
    21301 ctttttggtg ttagaaagaa taatatgttc attacactac ccaccgttac tgaaaactta
    21361 taaatattac tcaatattta taatgttact tttttgcagt aactttctcc tttcttattt
    21421 tctaagtatt cttaatacta tacgctgtaa caagcgtttt tcctttgctc attttaatct
    21481 tattatgctt tattaatatt tttatatggt ctataagtat cacttattct ctttttagaa
    21541 aatttactaa aaattaattg acttaaactt agccataata attttactct acactattaa
    21601 ttttaacata tttatctcat tattaatgat tttattttaa taattttaat ttattttgct
    21661 attaatttta tttacaaaaa ctgaaaaaac cgctaaatga ttagcggctt atctctcata
    21721 ttactagaac actaatatat atgaattttt aattataatc taactacgtt agcagcttga
    21781 ggacctttag ctccatctac tacttcgaac tcaacgcttt gtccttcttc taatgttttg
    21841 tatccatctc cagttatagc tgagaaatgt acgaaaacat ctccttcacc ttctacagat
    21901 atgaatccaa atcctttttc gttgttaaac catttaacta caccagtttt cattaataaa
    21961 tcctcctaaa aaaacacaaa tatttagcta ctcttttctg taaccttatt actactctat
    22021 catatatagc tatgtttgtc aattattatt atttttatac attaataatc aattttttta
    22081 ctttataatt ttaaatgatt attatctttt acactttatt tttctttata attttttaat
    22141 aatttatttt tattatatgg aatcttatgg acttatcact ccagcctaaa tcccatggca
    22201 ccaacattct atcagtatta tggcaagtta ccaaaggata tcctcttgag tctaatcctg
    22261 tcactgtcga aacatgtgtt attctgcctt tcttctcata accaacaaaa tcccctggac
    22321 gtaaattata agcttctttg tatacttctt cataggaacc tttagcaatt aatgaacctc
    22381 ttccactata cagtgcataa tttttaaaag cttgtgcatt tacccatgct tttgtacctg
    22441 ctctctcgct ataattccaa attgagttct ttttgaacct tccactttca aacattatct
    22501 gtgatgcaaa gtttgcacaa tctccacctt ctggattata atccctatag tttggattaa
    22561 atttcatacc atgttcttca tcagctgcgg ctccacagta tttatgtgca taatctatag
    22621 cttttttttg ttcatctgta agctgtaact ctacaccatc ttgttgacca atataatttc
    22681 ttatatcatc agattttata ttttctaaat ttaaagagtc tgcaaaaggg tctgtatacc
    22741 actctttagt tatgacatat tgattatcta taatttttac atttagataa tgatatgtac
    22801 ctattctaaa ggcattttct ttgtcctttt gattttcata tgaatactta tattcagtgg
    22861 atactgaaca gattatacca tagaggtctt tttctttttc ctttattttt ttaatcttaa
    22921 cccttgtctt tatgtcttta aattttacac cttgtttact agaccagttt tctaaatatt
    22981 tcatcttttt tacttcattt tcataagccc acagaccaaa ttttttatcc gtttcataaa
    23041 gaccttttaa aatattttca tttttctcta atattgcctt atttctgtag tcaaataagt
    23101 tttcaagcaa tagtttatat tggtctttta catcatcacc ttcaacattt gcagattcca
    23161 tgactttttc ttccatattt ttcatactta aatcattgat taaaaataca cctgctgtag
    23221 tagctaaaga caaaaataca aatgccatta tacctttgat gtacttattc ttatttttca
    23281 gtattttcaa agctacacct cccaaatagt acaccttaca aatcaaatta caaagaatat
    23341 tctgtcacat atagtaattt tcatatttaa taattctcta ttttattatg tccaatatta
    23401 aaaattaatt gtatcaatac actgtttttt ttacaatttt ttattttata atctactttt
    23461 ttcttttttt tatagtaata tatatgtata taaatatttt ggtatcatta taattaatac
    23521 atactaatgg agggtttttt atgcaaaaaa aagtcgcagc aattaatgac ttatctggaa
    23581 taggaaaatg ctcattatcg gttgctatac ctatactttc agcattaaag gttcaatgtt
    23641 gtccatttcc aacggccata ctttcaagtc aaactggtta tcctgaattt actttcttag
    23701 actttactga tgaaatggta aaatattcaa atgtttggaa aaatttaaaa gtcaattttg
    23761 atagtatata tagtggtttt ttgggttcaa agcatcaaat tgagatagtg gctaatttta
    23821 taaacgatta tcccaatgct tttatcgtag ttgaccctgt gatgggtgat aatggagtta
    23881 tgtatccaat attcacagaa gaaatgagac aggaaattaa agaattagtt aaacattcag
    23941 atttaactac tcctaattta acagaagctt gtttcttaac tggaaatgat tataccaaaa
    24001 gcgattataa tagagatgag cttatatata ttgcaaaatc tgtatctgat ttgggaccta
    24061 gcaaagtcgt tattactggt atattagaag atgataatat attaaatttg gcttacaata
    24121 gagataatga ccatgtattc tttacatcag tcaaatataa taattgttct tacagtggaa
    24181 ctggagatat attcacatct atactttgtg gtatgttagt aaataaacat gatttgggag
    24241 tagcggttaa tactgcaaca gattttatat ataaaactat aaattacaca tctcaatttg
    24301 atactgatag aaatgatgga gttatgtttg aaaacttttt atcagattta accaatatat
    24361 aaaatcaaaa aaactatcca ttttataaat acctataaat cataaaattt aggtatttac
    24421 aaaatggata ttatttacta aactattggt atttcaattg ttaccatagc accaccagtt
    24481 ttcacagaat tttcaataca taaagtccca ttatgttttt gtacaatatt atttacaata
    24541 tagagtccca ttccataatg agatttagaa tttctactca aatctcctgt ataaaactgt
    24601 tctgtagctt ttgacaaatc tgctttagaa aaaccacttc cacaatctgt aactacaaat
    24661 tttaagtatt tattatgtac tgatacactt atgtatagtt tactctggct tggtgaataa
    24721 tctatagcat ttgaaattat attcataata gctctgaata acagttctct atctatagtt
    24781 atatttttag gcaaattatc ttgtttataa aactccacat ctaagttttt agtactagct
    24841 aatgcttcta tttgcactaa aacatctttt ataaactcac tcatactgat tgcttcttta
    24901 tttaatatat agccattctc tgtttttgat atatctatta aagtattaat atatttttct
    24961 atttgagtga caccctttga gatatattcc atgtattcta ataactcagg attttcattt
    25021 atttcaatta ataagtcagt atttccatgt ataattgtta gtggtgtttt tatatcatgt
    25081 gccagtgcag atatctgttc ctttctattt tcttctagca tccattgttt ttctaaagaa
    25141 tcttttaacg ccaacttcat tttatccata gaaaaaagga cattattaat ttcataaatt
    25201 ttactagatt ctacaacaaa atctaaatcc tgttgttcta ttttttctgt agtatttttt
    25261 aaaagctcca tttcaatatt aaatttcttg ccaaatactt ttgataatat aacaatttca
    25321 ataaaaaata ttatactaaa aataatcata cccaatgtct ctggttttgg taaatatgct
    25381 cttaaaattg gagaactata ctctgatact attttgtatt ttattataaa aacttcatct
    25441 ttcttaaaaa actttatata actttctgaa tatgcatgcc tttcttcaat atctctcatt
    25501 aaattccata cttcttttga ttctttttta ttaaaggtcc ctgaaattaa attcccatcc
    25561 aaagtataaa ccccatactc atatatactt ggaattatat cttcagttac tttttcactt
    25621 gaaataatct gttctttata tttattaaat tgtttttctg cataattagc tggtaaaatt
    25681 acacctgatg ataataatac agaaaatgat aacattaaaa ggagtacaat tgaaattgtc
    25741 ataataaaga ataaagataa atattttata aaaatagttc ttaacttttc aacttttttg
    25801 ttgttagatt ttttacctcc aagcatttgt accctgtcct ccaatagttt aattaattca
    25861 taattaatac ccaaccagtt ttttatttga ttattttata tctttttatt gctaagttat
    25921 attttaacat aataatataa ataaacagtc aatttttact ctattcttct gattttttac
    25981 cttcccattt agaaaaccaa atacataaaa ttacaaatgc taaaattgtt acaaaaccac
    26041 aaattaaacc tgttgattga tatgctttta ttgattcaac ctttatatat tcaaccgttt
    26101 tagaacttga aaaagacgac caaatagtta taaatctaac accccatgca caaggaaaat
    26161 actgccaaat accagaaccc aatccagtta ataataatgc tgaaagtaac atttcaacta
    26221 tacctactcc aattgatgtt ccttttccga accttaaact taagaataaa tgtaatatgt
    26281 attcaaataa attacaccca attaaaatta gaattgaaat catataaaag cttgatgtaa
    26341 agtaagcctt agatatacca aatttaaaac ctaatacaga cactaatgtt gctacaaaac
    26401 caagacatac taagattata tatttactta taagagtaag atattttgga ttagatgttg
    26461 ttaacatatg tttataattc cctgcataat attcttgttc aacaaacatt gaacacacaa
    26521 cacttgacaa taaaggaaaa gctattgcta taacttgtaa atatgctact atctttagta
    26581 tattatcagc tcctgaaacc ttataatacc ataaaaacaa tcccatcccc attaaagcaa
    26641 tgcaaatgtg cattagtatt ataaaatttc tctttaattt tattaaatca gaacaaaata
    26701 ttcttattaa ctgtctcata ttacttcacc tctctattat tgaaccaaac ccttgtagct
    26761 aaagcaaata atacaaataa aacaatagat attaatactc ctggcaaaat aacagcatta
    26821 tttaaaaatt cacttccctc tggtacagga agaccatttg gcagaatatg caatacaaca
    26881 gccattaatc ttgaaggaat cgaatatgga aacatatacc acatttctgt agttgagaaa
    26941 acagcaactc caacaatagt aaagaacata tttgttagaa ctgtaacaaa cataccaaat
    27001 tttgaagcta aaaacataca aagaggaatc tgccacataa atagtaaaat aattaatata
    27061 cttgcaatga tattttgacc tattgaaata gtttctttat agattatccc aacaaaaact
    27121 acacctattg cagaaataat tgatgctata aaacaacaaa taatacagta aatcattttt
    27181 ccagtccaaa cgtctttcat ttcaattggc aatggtaaaa taccacgata tttacttttt
    27241 tcatctttta atactattaa agaagatatt aatgttaatg ctcctggaag aaatgtcgta
    27301 taccaccagt tgaaagcagc attttgccct ccacctagtc cacacaaaag tatacaaaac
    27361 actggaacta tccatatcaa cttcattcca aaagttcctt tcatcttttg aaattccact
    27421 ctaataaatc tttccatatt actgccctct ctttctattt tcagctacaa ctttcataaa
    27481 aagttcttct aagttttgtt cttttgatac ttctccttga taacccatta ctccattaga
    27541 aattattcct atttcatcta caacttgttc aacttcagac aagatatggc ttgataaaat
    27601 aactgtaatt ccttgttttg gaaaagaacg aatcaattct ctaagttctt caataccaat
    27661 agggtcaaga ccatttgtag gctcatctaa gattaaaagt tttgggtgat ttaataatgc
    27721 aatagcaata ccaaggcgtt gcttcatgcc tagagagaat tgacctgaac gctttttaaa
    27781 ggtatctctt aaatctacag tctctaaaac ttcttcaatg cgagagtcag gaagaccaag
    27841 taaagtagta tggactttta aattttctcg tgctgttaaa ttctcatata tagcaggaga
    27901 ctcaataaga gaaccaatat ttgataaatc ctttctagtc catctatgtc cctcaaacat
    27961 tatttttcct gaagttggat ttaaaatccc agttatcatt tttaataaag tagatttccc
    28021 agctccattt ggtcctaata atccatatat agaattagat ttaattgcta gtgaaatgtt
    28081 atcaactgcc atctgctcct taaatttttt agaaactcct tttacctgca aaatataatt
    28141 actcataatt ttcatccttt cttttgtcaa acttatttac aagaatcatt ctaaatcata
    28201 aatataagga ttttataaga attaaaaaac aaaaaaagat gtgttaaaaa cacatctttt
    28261 tttatcttaa ttctattaat tttccattgt agtatatctc tacttcttca atccaaatgc
    28321 tattatcttc ttcttggatg acatttttaa taatattatt tatagtatat attatatttt
    28381 gttcattcaa ataatcatct ttccaagaaa ctctactttt tttatctggt tctatcaagt
    28441 ttattttaac aagattatct ttttccttaa cttctacttc catgtctaaa ccattaaaat
    28501 actcatttga taatgtatct aagattaatc gcagtttatc cttaagtgaa ctttcttcat
    28561 caatctgtaa gtactttact attttctcat cctttgaaat tatctctata ggtattttag
    28621 tagttttttt agaattaatc attttactag ttgtttcttc ttttgcatct tcatccttct
    28681 ttacaactgc tgattcttta gaatcaacta gctcaactgc actgatgcta taatccatac
    28741 atgccgtact caataaagta gtagcaatta ttaaaaaact tactgctatt ttattcataa
    28801 gtatccctct cctaattctc tttcgatact tattatacca tagtatagat tatattttta
    28861 tacttttaaa tcaatttata aattattata gtaaattatt tacaattcta agctatttga
    28921 atttattttt atagtatatt ctttctctaa attcgacaga tttttattca aaagcccttt
    28981 tatttccctc gtatttctat acttgttgct atgttcttca ttatgttcat aatcatatga
    29041 atcattgtca tattcttcat cattattaaa ttcttcaatt gcttcttttg actttatatc
    29101 acaattaaat ttatacttat ttactggtat atcaagtctt accttagaat ttggaacttc
    29161 tatgtccagg aacttagcaa ttggcatatc agagtacaag taaaattcac tttcataatt
    29221 tgaatttcta cctattttct cttttatatt tacattttca ggtatatact cttctatatt
    29281 attgataaat acatctccat tctctgaaga tatatccaat gactctgatt ctatgtttac
    29341 atttttaacg cctaaaattg actttgttga tagatgaaga ttgctattag atattaagtt
    29401 taagttttct aatctattta ctttattttc tgttatagct ctgctaaaac ctccagaaga
    29461 agttttatat gttatattat ttgaatctac atcatcagat ataaataagt acgaacttat
    29521 acttgatgca tttatatcta cattatttgg tacatatatt ctaatttctc tataatcacg
    29581 tgaaaacata ctatttatag atgtatttaa taagtggtcg aaacttctaa tctttttaat
    29641 tttattttca gattttcttt cgccttttac aactaaagtc ttatcttgta gggttacact
    29701 gtatttgtaa aattcattat tccctcttgt tgtcacacgc acatcttcat ttgaactttt
    29761 tcttatcata acatttatat tgtcaacagc tatatctaac ttgtcaatat ttactttttc
    29821 ttgataaagt ttgttttctt tgtttaattc agcttccttt tctaaaccaa agtttattag
    29881 gcttggcata actaaaatcc ctgaccatgt tgtccctatt atacctatta ccaataaaac
    29941 tgcagcaaat atagcaagtt ttttattcat gtttatcttc tcctttctta ttttctatat
    30001 cttcaaggtc cttatatatc gcctcaaaat ttatactatc atcattatat tcatcagttt
    30061 cttttatgct ttcttcttct ctattgtagt aatcattatt atatgaattt tcattaatgt
    30121 ctttattcaa atcttcatca tttaaagtag ctttttttct tttttccatc ttttcatttg
    30181 cctttatata aatatttttt ctctttatcc aatgtatata attatttagt aattgtttac
    30241 ctagttttat ggcaaataat aaaatttgcc atgcaagtat ctcaagccct acaaatgcta
    30301 tggttgcgaa aatacaaaac atcataacat ctggaaccat tgctataaat ttaatcacaa
    30361 atggaattaa tataacaaaa gcaattaatg agcatactaa tgcaatagta gcagatacaa
    30421 tagcacatac aaacgcaaat gctggaacaa ttagaaataa agataacact actaataacc
    30481 cctttataac ttgtctggat aatcctccta caccttcttc tatacttggt gtaagtttat
    30541 catttatatt ttcttttgat ttattgtact tttccttagc ttcatctttc aagatagaat
    30601 aatccttttt tacatttgat ttaaatctac taaatttacc atttaatatg tctgtattca
    30661 ttatttttcc atctatattt tcttttgatt tactatactt ttcttttgct tcatctttta
    30721 agatagagta accttttttc atatctgatt taaatttact aaacttatca ttcaaaacat
    30781 ctgtatttat tattttttcc tcttcaatat ttttttcttt agtttgtaag ataagttctt
    30841 ttgctataga ttttggtgag ccaagtgatg ctattatctc catatcactc tttccctcta
    30901 taagaccatc tacgaaatac tcttcataat ctctcaatat atcattaact tcgctctcag
    30961 aaaaatcttt ttttagataa tcctttaaaa tttcaagaaa ctcagtttta ttcaagttca
    31021 ttacctccct catcttcact tattaaaatc tcaacagcct ttacaaattc tctccactct
    31081 tccatcagat tggataaatt ctcttttcct agtgaagtta ttctatagta ttttctagca
    31141 ggaccttcat tagattctag tatatatgtt tcgaaataca aatcttttgt aagtcttctt
    31201 agaataggat atattgttcc ttcatttaca tctattactt tagagatgtt atgtactatc
    31261 tcatatccat acatatcttt cttagatatg agagcaagta cacaaatttc taaaacaccc
    31321 tttctaaatt gtgtgttcat atactccatc ctttctttaa atacacagta ttgtgtttta
    31381 caaggtacta tgttaaaact tcttatatcc tcataataac acagtactat gttctgcaca
    31441 atagttattt ttaaatattc ttgttgatta aatccttcta taaatgtata atactctaat
    31501 cagatatatt ttatgaaagt ggtgataaat tggctaaaat gaaagatgat aaaacaatat
    31561 taatcctaac tgctcaattt ggtgctggac atataagtgc tgcaaaagca gtcaaagaat
    31621 gtattattga aaaatatagt aactataatg ttgttataca aaactttata aatgcaagta
    31681 ttcccatgat gaataagcct atggtaaaac tttatgaaaa caatacaaaa tatactcctg
    31741 gattatataa ttactattat tactttaaga aatcgtttga ttcaagacat gatttatctc
    31801 ataaattata tacacctaaa ctttctgagt atattgctga tataaatcca gatttgatta
    31861 tctctacatt tccactagct gctgcttgtg taaataactt taagataaaa aatcctgata
    31921 taaatatacc tacacttaca gttataacag atgttgtaga tagtatggaa tgggtttttg
    31981 aaaatacaga tttgtatttt gttccttctc ctgaaataaa aaatagattt tttcagaaag
    32041 gaataaatcc agactccata aaggttactg gagttcctgt tgataagaga tttcaaattg
    32101 aaagtaaaga actatgttgt gataaatata gactattact tctaggtgga ggaagaggtc
    32161 tatttgatat agatgaggat tttatgcatt ggatagatga atttattgaa gaacatagta
    32221 attctataga aattacaata gttactggta aaaacaaaaa attatatgac aatctaactc
    32281 ataaaaaacc tctaaaaaat attaaagtac ttggatttgt aaacgacatg tataatctaa
    32341 taagagaatg tgacctaatg ctaactaagc ctggaggagc aactatattt gaagctattc
    32401 aatcacaaac tccagtactc gtaaaaatgc caaaagttgg acaagaaatt gaaaacgcta
    32461 aatttataat agataagggt cttggcatga tttatagtga tgacctagac cttaaaaaca
    32521 tattttataa attagtatca aatgaatttg attctataat aaactttatg aaaaaaaatt
    32581 taaaagaatt taaaacagtc attcatccag agaaaattgc tgattatata tcagaattaa
    32641 tagataatca ttacagttaa attgtactat taaaaaattt caaacataaa attaaaggca
    32701 atcaagtatg cttttacact tgagaattta ctcatagtgt ttaataatac ttgattgcct
    32761 ttatcatatc aaaatcaaat tgttatatta gtatatattt ttcttgtact gcatatacag
    32821 ttgttgtatt tcttccatac ttctctttaa ttctatttgt aaatctgaag cagtctggta
    32881 atccttattt tctatagctt ctctcactct agtgaatagt gattctctag ttaaagtatc
    32941 cttcataata ttgaacttta aatccgatat tgagttccaa actacctcat caagcgcatc
    33001 catattttct tttgtgtgta tcttgacaat acttcttatt ttttgaatcc cagcctctat
    33061 tattctagtt tttaatttct tttgccactt atctatagct ccaatcttaa atgattctat
    33121 tatactatca gtaaatacat caccttgttt taaagatttt aattttgaag catatatttc
    33181 taagtttttc atattttcat aaactgtagc cggtggtatt ccaaatcttg catttctttc
    33241 ttccaaacta taatgttcaa atacatcatt ttcatctcta tatgctctat ctttttctaa
    33301 atagaaactt tcttcaccta catttttaga taactctttt tcaagttctt ttgtactaag
    33361 accactctta gctgctgctt ttataccatc caacattgtt tgataacatc ctgctattac
    33421 taaatacgtg ttagatagag ggcttggtga ccttaactca aatcttacag tctttggatt
    33481 tttcatatct cttattaatc caacaagcac tgacctattt cttgatggaa tctcataact
    33541 atgacctaaa gatgtaacta tgcatacagg agcttcaaat ccaggaacta atctattaaa
    33601 tccatcatta gaagctgtta caattgggtt taatacttca taattgtaaa gaagtcccat
    33661 aagagcccca tatcctagtt cacttagata atcttctttt aaatcttttg gcgcaaatag
    33721 attttttata cttccatcct ttaacttcgc acttactcct acatgtgtat gtccaccact
    33781 tcctgctact ccatgaattg gttttgcttt aaatgtaact tctagtccat gtgatgtaaa
    33841 tacatcctct ataacatctc ttactaataa ttcattgtct gctgcttgta acggtgtaga
    33901 aaacttccaa gaaacctcta attgctccat agcatggttt gttcttccat ctatactaat
    33961 agaactttgt attcctccga cctctttgtg agccatttca gggtttacac ctagcttttg
    34021 aagtataatt aaacttttct ctaaacaagt tcttataata ccatgagttc ttttccagta
    34081 ttgctctttt aggctttgtg atacatataa tttctctaag tcagctttat cctcaggagt
    34141 attaacccag aactcaagtt ctgtagctgc tgttaacatt atttcctcta tatcatctac
    34201 tgaatctatt cctatgttat ttattacatg tggatattct ctaaatattt catacattga
    34261 ctttttaaag tacttatctg ctttttgaag tactcctcta gaacatactt ttttattatc
    34321 atgtattaaa aatgcaggta tctttaatgt tccaacaggt agacctactt cttcgtctat
    34381 atattccata ttgtaatcta cataccaatg acaactttta tctggcatta aatctacttt
    34441 cgcattattt aatgtagcta tattataaag ctcaacactt gaaccatcag tctgtatagc
    34501 tgattccaaa aatttatcta tatcctccaa aaataattct acaggaatct tttcatctgt
    34561 tgcatttcct cctaaatcaa cccccattaa ggaaacaaat cttatatttt tattgctttt
    34621 taggatttct cttaattctt cattagtgtg tttatctttc tctattacgt ataatagact
    34681 atccattttt ccactctccc ataattttta tgtatatttt attttgttaa atacaagata
    34741 aatttcattt tccttacatt tatgaatttt tcttgcaaat tattcgtgtt ttattcgtgt
    34801 ttttatttat attattatat atcaagtata aaaagttcgc aatatttttt attggaaaaa
    34861 atattataca tacaatagtt t
//
LOCUS       DAMJYX010000030        34871 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJYX010000030 DAMJYX010000000
VERSION     DAMJYX010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.030"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC479_001607"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC479_001607"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2804155.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC479_001608"
     CDS             complement(2851..3216)
                     /locus_tag="PC479_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2804156.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC479_001609"
     CDS             3415..4263
                     /locus_tag="PC479_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804157.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC479_001610"
     CDS             complement(4700..5788)
                     /locus_tag="PC479_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804158.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC479_001611"
     CDS             5913..6641
                     /locus_tag="PC479_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2804159.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC479_001612"
     CDS             complement(6843..7334)
                     /locus_tag="PC479_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804160.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC479_001613"
     CDS             complement(7345..7788)
                     /locus_tag="PC479_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804161.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC479_001614"
     CDS             complement(7778..8617)
                     /locus_tag="PC479_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804162.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC479_001615"
     CDS             complement(8836..10221)
                     /locus_tag="PC479_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2804163.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC479_001616"
     CDS             complement(10394..11137)
                     /locus_tag="PC479_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2804164.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC479_001617"
     CDS             complement(11157..12419)
                     /locus_tag="PC479_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2804165.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC479_001618"
     CDS             complement(12603..13601)
                     /locus_tag="PC479_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2804166.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC479_001619"
     CDS             complement(13585..15096)
                     /locus_tag="PC479_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2804167.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC479_001620"
     CDS             complement(15150..16112)
                     /locus_tag="PC479_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2804168.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC479_001621"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC479_001621"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2804169.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC479_001622"
     CDS             complement(17092..18105)
                     /locus_tag="PC479_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2804170.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="PC479_001623"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="PC479_001623"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2804171.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19517..19780)
                     /locus_tag="PC479_001624"
     CDS             complement(19517..19780)
                     /locus_tag="PC479_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804172.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="PC479_001625"
     CDS             complement(19850..20923)
                     /locus_tag="PC479_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2804173.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21171..21929)
                     /locus_tag="PC479_001626"
     CDS             complement(21171..21929)
                     /locus_tag="PC479_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804174.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(21926..22843)
                     /locus_tag="PC479_001627"
     CDS             complement(21926..22843)
                     /locus_tag="PC479_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804175.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22843..23043)
                     /locus_tag="PC479_001628"
     CDS             complement(22843..23043)
                     /locus_tag="PC479_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2804176.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23048..24142)
                     /locus_tag="PC479_001629"
     CDS             complement(23048..24142)
                     /locus_tag="PC479_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804177.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24652..24792)
                     /locus_tag="PC479_001630"
     CDS             complement(24652..24792)
                     /locus_tag="PC479_001630"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2804178.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(25102..26409)
                     /locus_tag="PC479_001631"
     CDS             complement(25102..26409)
                     /locus_tag="PC479_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2804179.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26474..26953)
                     /locus_tag="PC479_001632"
     CDS             complement(26474..26953)
                     /locus_tag="PC479_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2804180.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27000..27863)
                     /locus_tag="PC479_001633"
     CDS             complement(27000..27863)
                     /locus_tag="PC479_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2804181.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27863..28618)
                     /locus_tag="PC479_001634"
     CDS             complement(27863..28618)
                     /locus_tag="PC479_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2804182.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28645..29133)
                     /locus_tag="PC479_001635"
     CDS             complement(28645..29133)
                     /locus_tag="PC479_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2804183.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29151..29552)
                     /locus_tag="PC479_001636"
     CDS             complement(29151..29552)
                     /locus_tag="PC479_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2804184.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29555..30052)
                     /locus_tag="PC479_001637"
     CDS             complement(29555..30052)
                     /locus_tag="PC479_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2804185.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30076..30480)
                     /locus_tag="PC479_001638"
     CDS             complement(30076..30480)
                     /locus_tag="PC479_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2804186.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30710..33277)
                     /locus_tag="PC479_001639"
     CDS             complement(30710..33277)
                     /locus_tag="PC479_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2804187.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33443..34186
                     /locus_tag="PC479_001640"
     CDS             33443..34186
                     /locus_tag="PC479_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2804188.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            complement(34498..34704)
                     /locus_tag="PC479_001641"
     CDS             complement(34498..34704)
                     /locus_tag="PC479_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804189.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT        11295 a         6379 c         3559 g        13638 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatatttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagcttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaagccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgctcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaac cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcataattca aagaaaacct agagatttct ccatgatttc
     8521 tttttaaata tttacatagt ccacaatagt aagctttgta ataatcgtat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttgtgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttattta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg taactaacat ctccaatatc caatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg tacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg ggattcttat aggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcctct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta cccagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttaata gtggatactt tttgagacaa
     9961 actatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccatagct
    10021 agccgcaaat gctgtatcca taccttctaa ttcatctata ctaaaaccat atttttttaa
    10081 gtattctttt tgtaaatttg aagcataata ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattctcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctatc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgtttag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccgaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaatcc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattaa tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 tttttcccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gtccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacctgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatctacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattcc cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaaaat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcttcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcccc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattctgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tatttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattacact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg taaccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaagt tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc acctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat gagatgattg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaataatat tagtaaatgg attatacata ttattattaa aacagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacctc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcaacttg gtcattcttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagtatatt ttaacataat
    21001 taaatattct tatatttatt tgatacaaat aatattattt gtattatttt ctacacttaa
    21061 ttctatatat taaaaatcaa aaagacttga aacttaagtc caagtctttt tgatttttag
    21121 gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta ttaaactggt
    21181 tttttattaa ataaaataac agctgctagt aaacatacta cagaaataag aactgttata
    21241 ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc tatatttttt
    21301 gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat taaactcaat
    21361 aaaagagttg ccaccaatac aagtaaaaca ctggcaaatg ctggtctaaa tagtacacac
    21421 ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa tgctgaaaat
    21481 ataatatgat taagatttgc agttggccaa aaatatattg tatagccata agcaaataaa
    21541 aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt agacaatact
    21601 atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt agaatattca
    21661 tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc agagatattg
    21721 ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt taaagtcggt
    21781 gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact tataccaaat
    21841 attagaaata aaataattaa actaaataat ttataatttc ttaaattctc tttaaattct
    21901 ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa ttttcaagtg
    21961 ataattctat tagttccatc tttactggac aaagattcat ttctgcgagt gtagatataa
    22021 ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata ttagtttctt
    22081 ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga atttcatcat
    22141 tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt tctgaaagtg
    22201 taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag attctttcaa
    22261 catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt ttaagcataa
    22321 tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca tcacaaataa
    22381 gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc attccacgag
    22441 aaaacccacc tattcgtctc ttttcatttc caagacccac aagtgataac aactcttcac
    22501 tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga gaaagatatt
    22561 caagtggtct catatagttg taaaactctg gaacatcagg cagataacct acatgacgat
    22621 tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt ccactatctg
    22681 gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca ttcttaccta
    22741 caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt atctttcgat
    22801 taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt cacctcttcc
    22861 tattgtaaaa tacaaaattg gtcctattag actaataaac agtactacaa taatccatat
    22921 tgccttattt ccaattttat aatttggatg tctcaataca tgaacaagtg cagtaattat
    22981 aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca tcaaattttc
    23041 cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat tctttgtttt
    23101 cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct gcttttacga
    23161 ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca agtttattaa
    23221 aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa tctacatatt
    23281 ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt tgtgcctgtg
    23341 ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat atatctgaaa
    23401 ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt tgcaaaatac
    23461 ccactaggtt ttggactaat gatataaggt tctgatatgc accaagttga aatgtttctc
    23521 tcgcttcatc catttgacca ttcatcattt gcccctgtgc aagaagtata gattcattta
    23581 tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga tttaacaata
    23641 tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca ttacaagttt
    23701 cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc tttttttcat
    23761 ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa ggaaaacatg
    23821 aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat ggtttaaccg
    23881 taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc atttgaggtt
    23941 cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc ggtaaaaaag
    24001 ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta gaaacgccta
    24061 tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa atcagcgttt
    24121 ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat ccttaaacat
    24181 gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca cagagaaaca
    24241 aataaacaag tatataaaat atatatttaa atttatcttt aatctctttt gtaaaatatg
    24301 taaactttgt aaataaaatc catattatat aaaactaata gttaatctat ctaaatacag
    24361 tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat aaacacctta
    24421 ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc gattttccat
    24481 ttaattcttt tataaagtta ttattcgtat ttatttctct aattataaca ggcaactatc
    24541 atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc aatcatttag
    24601 attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt attattctcc
    24661 attttcatcc caagtcataa atccccatcc actatctgga taagtatcaa tagacttttc
    24721 tctaatcata taaccaactt tataacactc ttttgttatt ttattagata gtatacatcc
    24781 ttctccattt atctaattcc attcaattaa cttttctttt caaccttaac ttttataaaa
    24841 tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat gagggtgtct
    24901 caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa tatattttca
    24961 tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat ttgatggtgt
    25021 tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc tgtaaatttg
    25081 cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca aagtatatat
    25141 ctccatattc tttcgtatat gatttattgg tactctttca tctactccat gaccaccagt
    25201 tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac ttgcatatgt
    25261 gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat aggcttctaa
    25321 taatttttta agtactaaat tactctcatc cacataatgt aagtctttat gagcaataac
    25381 ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat tgtttaaaac
    25441 tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat ttacttttaa
    25501 tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa tccctaatgc
    25561 atctccataa taatcactac aaaggtattt tgaaactaat tcgatatctt ctctatgctg
    25621 aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat tttctccctt
    25681 ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa taattccatt
    25741 aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc cccttactat
    25801 tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat gaacccttcc
    25861 tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac ttggaatttc
    25921 ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat ttgtaccaaa
    25981 aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt tcaatacatg
    26041 tataagcata gatacagcac ccttattatc tattacacct cttccataaa taatattgtc
    26101 aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa ctatatctaa
    26161 atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc caatataatt
    26221 atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt tatctaaaga
    26281 ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt gtgaatcaat
    26341 ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta cacttctgct
    26401 tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa gaacaggctt
    26461 ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca tcatgagcac
    26521 atttttgagc aaaatctaat ttaacacctt gtgcgcagtc aactcttcca taatccccat
    26581 aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat tctttccaag
    26641 cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta ttttctacta
    26701 attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc atttttcctg
    26761 gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat attgcttctt
    26821 tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga agatagaacc
    26881 atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt tctgtataca
    26941 ttttttctga catttctttc tcctcctaaa attaaattct atattttaat cactgtgaat
    27001 tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac taataactta
    27061 gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac accaagtggt
    27121 aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt accaccaata
    27181 tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc catcataaac
    27241 attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga tagaattttc
    27301 ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc ttcgcaaaat
    27361 agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc tgctccaaat
    27421 gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt atctccaatt
    27481 ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc tgggattttt
    27541 ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa tcctccccat
    27601 atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa atcttctttt
    27661 ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa tgcttgcatt
    27721 ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt ttttgttaca
    27781 tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc atttttaact
    27841 tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct ttcataaagg
    27901 taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat atttcaaaat
    27961 attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta cctatagtaa
    28021 atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt aatccattca
    28081 ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca aaaaagttac
    28141 atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa agtgttgcac
    28201 atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta tgaaccaata
    28261 cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct aaaggtaccg
    28321 caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt attgcaataa
    28381 gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt cctagataaa
    28441 ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa agtaatccaa
    28501 ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac cctgcaagac
    28561 ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca gattccattt
    28621 ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt catatgtcat
    28681 agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat taagttcatt
    28741 aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt tctttcctgc
    28801 ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt tataagcctt
    28861 atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta aagtgttgtt
    28921 ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc ctgatggtgc
    28981 tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat caactattat
    29041 tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat gaattaatct
    29101 gtggtctact cttactaatg ttatttttgc cattatattc acctcataat ttatattttt
    29161 ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat tctttctggt
    29221 aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat tgtctgtaag
    29281 ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc aacacttgat
    29341 gtagatcctc catataaatc tgatattata attttcacat cttctttatc tactacttct
    29401 tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa aacttcatat
    29461 tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc ccaagttcca
    29521 tggcacagta tgattacctt atatttattc attgctaaat aaactctcct ttatagcgat
    29581 taatattttg gtttaattct acttcttctt catcaggtac catttgaatt gatacattaa
    29641 tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg tatacatttt
    29701 gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt tttttgatat
    29761 taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat acaaaaacgt
    29821 tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt aatattgata
    29881 aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca tcattggttg
    29941 tttcatcatc tactactact atctttttta cacctagatg cttaacccat ttatatataa
    30001 cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac atttttaatc
    30061 aaactccttt ttctattaca ttgagctaag aactttgtta atattgttta taccagtttg
    30121 tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc tattaattaa
    30181 agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc cacctttttt
    30241 aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta atactaaaac
    30301 tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta agtcttcaat
    30361 attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg caataagttc
    30421 acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta ttcctatcac
    30481 caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc caacttatgt
    30541 gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttatt caattttttt
    30601 taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc aaatctgtat
    30661 caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat caacttttga
    30721 atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat tccatatctg
    30781 aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata actaaatata
    30841 attctttatt tctactaatt ctttcttcat atgatttata aactaaagac tcttttatta
    30901 ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt tcatcttgaa
    30961 ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata aaataagcct
    31021 tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat ctatcaaaat
    31081 atctaccacc attatctttt tcagcatatg taacttccat atctccataa tgtttcttta
    31141 tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt agagcctttc
    31201 tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact tcttttcctg
    31261 tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt aaaattaaaa
    31321 ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa atatcctgaa
    31381 ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt aaagtattgg
    31441 ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat attgctatac
    31501 tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa attgcttcat
    31561 cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct tttgctatgt
    31621 tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt actatatttc
    31681 caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt attaatttat
    31741 ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt acagtagttg
    31801 caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta tatataaaag
    31861 tattcattaa attataggaa tacttctgta tgtctccatc tataacactt ctaatttgtg
    31921 actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt ccataaaaat
    31981 ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc ttttcttctg
    32041 gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac tcctcttttc
    32101 tattatttcc atataatgat tctctaatat tatttggtag aagagagagc cttacatata
    32161 cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt aattgtatat
    32221 cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc ttaataactt
    32281 cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa aaataacata
    32341 taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt atagttacag
    32401 gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta gtggctgcaa
    32461 ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta tatatccctt
    32521 tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga acttcatcta
    32581 aaaacaatac tccattatta gcttcatgta caataccata cttatcttta tcagctccag
    32641 taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga ttatcagaat
    32701 aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt atagttaaac
    32761 tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt cctattatta
    32821 atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct atttgggatc
    32881 ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt atactctcta
    32941 aaatatactc atctttgata gaatcatttt cactatattc gtttatagag ttaatattat
    33001 caaatatccc tttatttagt ttctttccta aaatactctc aatactttct ttatataagt
    33061 actgaacagg cttacccttt atctttataa tgatactttc ttttattaat tgatttaatt
    33121 ccttactagc attacttcta tttataccta cttcagtagc tatatattct gcatcaaatc
    33181 caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc aagcaaatta
    33241 aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc tataagcagt
    33301 aatactgtta aacatttatt attaagctta attctacaat attttaatat ttttgtctaa
    33361 ttatgtaact attgattttc tttcctattc gttttataat ataaattacg ttaccctcat
    33421 cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc actctatagt
    33481 cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag aaaatggaat
    33541 taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc acagatggta
    33601 ctttcacaac attcaaaata tcccaagaat cataaataac attataattt taagaggctg
    33661 tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg taattcctcg
    33721 gttagattat cttatcttgt catttcacga agctgtgttt tctccaaata cattagagaa
    33781 taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa tattaggaca
    33841 ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag ctatagaaaa
    33901 gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa atggtagctc
    33961 ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa tcatactaac
    34021 ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa atatcttaaa
    34081 ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta ttaatcattt
    34141 tcatcaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa taatacatgc
    34201 ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata aagaaattat
    34261 aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga ttgatatggt
    34321 ctatgatagc aatagaaatt ataacatttt taataagctt agttttatat accttatagt
    34381 ataaagctaa aaaaaagaat catctaaaat aaagtaaatc ttatataagg tttactttat
    34441 ttttatttgt aataatcatc aatacaaatt attttattat atgtaattat ttatgtctta
    34501 accaatttta tcgaaaataa cagtcttctc ttttaaagag atttttccta cttgatatcc
    34561 atattcattt aattcctttt tatatttcaa aaacgaatgg tctatctcta tcccagcaat
    34621 atcttttatt tcttcaaaag ttaatttaaa cgattgacta ttgtttttct taacatattc
    34681 ccataaagaa ctatatttac tcatcttata tcactccttt taaaatcata tcttatctat
    34741 taatttatat tctatcatta ttaatgtata aagtcatgct acttattatt tatatataaa
    34801 aatatccaaa taataagtag caatttttta tagtcataat atttttttca gattaaataa
    34861 aaatcaatta t
//
LOCUS       DAMJYX010000031        32208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJYX010000031 DAMJYX010000000
VERSION     DAMJYX010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.031"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     regulatory      153..243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            652..969
                     /gene="flgB"
                     /locus_tag="PC479_001642"
     CDS             652..969
                     /gene="flgB"
                     /locus_tag="PC479_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2804190.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            977..1381
                     /gene="flgC"
                     /locus_tag="PC479_001643"
     CDS             977..1381
                     /gene="flgC"
                     /locus_tag="PC479_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2804191.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1391..1708
                     /gene="fliE"
                     /locus_tag="PC479_001644"
     CDS             1391..1708
                     /gene="fliE"
                     /locus_tag="PC479_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2804192.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1733..3283
                     /gene="fliF"
                     /locus_tag="PC479_001645"
     CDS             1733..3283
                     /gene="fliF"
                     /locus_tag="PC479_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2804193.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3296..4366
                     /gene="fliG"
                     /locus_tag="PC479_001646"
     CDS             3296..4366
                     /gene="fliG"
                     /locus_tag="PC479_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2804194.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4359..5090
                     /locus_tag="PC479_001647"
     CDS             4359..5090
                     /locus_tag="PC479_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2804195.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5094..6413
                     /gene="fliI"
                     /locus_tag="PC479_001648"
     CDS             5094..6413
                     /gene="fliI"
                     /locus_tag="PC479_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2804196.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            6445..6891
                     /gene="fliJ"
                     /locus_tag="PC479_001649"
     CDS             6445..6891
                     /gene="fliJ"
                     /locus_tag="PC479_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2804197.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6919..8124
                     /locus_tag="PC479_001650"
     CDS             6919..8124
                     /locus_tag="PC479_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2804198.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            8142..8750
                     /locus_tag="PC479_001651"
     CDS             8142..8750
                     /locus_tag="PC479_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2804199.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            8777..9760
                     /locus_tag="PC479_001652"
     CDS             8777..9760
                     /locus_tag="PC479_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2804200.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9773..9976
                     /locus_tag="PC479_001653"
     CDS             9773..9976
                     /locus_tag="PC479_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2804201.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9976..10794
                     /locus_tag="PC479_001654"
     CDS             9976..10794
                     /locus_tag="PC479_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2804202.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10784..11479
                     /locus_tag="PC479_001655"
     CDS             10784..11479
                     /locus_tag="PC479_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2804203.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11491..11934
                     /locus_tag="PC479_001656"
     CDS             11491..11934
                     /locus_tag="PC479_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2804204.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11949..12323
                     /locus_tag="PC479_001657"
     CDS             11949..12323
                     /locus_tag="PC479_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2804205.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12324..12989
                     /gene="fliP"
                     /locus_tag="PC479_001658"
     CDS             12324..12989
                     /gene="fliP"
                     /locus_tag="PC479_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF2804206.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            13002..13271
                     /gene="fliQ"
                     /locus_tag="PC479_001659"
     CDS             13002..13271
                     /gene="fliQ"
                     /locus_tag="PC479_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2804207.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            13283..15097
                     /locus_tag="PC479_001660"
     CDS             13283..15097
                     /locus_tag="PC479_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2804208.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15106..17181
                     /gene="flhA"
                     /locus_tag="PC479_001661"
     CDS             15106..17181
                     /gene="flhA"
                     /locus_tag="PC479_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2804209.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17156..18172
                     /gene="flhF"
                     /locus_tag="PC479_001662"
     CDS             17156..18172
                     /gene="flhF"
                     /locus_tag="PC479_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2804210.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18169..19047
                     /locus_tag="PC479_001663"
     CDS             18169..19047
                     /locus_tag="PC479_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2804211.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19061..19762
                     /locus_tag="PC479_001664"
     CDS             19061..19762
                     /locus_tag="PC479_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2804212.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19778..20200
                     /locus_tag="PC479_001665"
     CDS             19778..20200
                     /locus_tag="PC479_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2804213.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20185..20370
                     /locus_tag="PC479_001666"
     CDS             20185..20370
                     /locus_tag="PC479_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2804214.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20411..21184
                     /gene="flgG"
                     /locus_tag="PC479_001667"
     CDS             20411..21184
                     /gene="flgG"
                     /locus_tag="PC479_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2804215.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21205..21966
                     /gene="flgG"
                     /locus_tag="PC479_001668"
     CDS             21205..21966
                     /gene="flgG"
                     /locus_tag="PC479_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2804216.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21989..22870
                     /locus_tag="PC479_001669"
     CDS             21989..22870
                     /locus_tag="PC479_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2804217.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22873..23247
                     /locus_tag="PC479_001670"
     CDS             22873..23247
                     /locus_tag="PC479_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2804218.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23279..24682
                     /locus_tag="PC479_001671"
     CDS             23279..24682
                     /locus_tag="PC479_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2804219.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24798..26735
                     /gene="htpG"
                     /locus_tag="PC479_001672"
     CDS             24798..26735
                     /gene="htpG"
                     /locus_tag="PC479_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2804220.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27123..28253
                     /locus_tag="PC479_001673"
     CDS             27123..28253
                     /locus_tag="PC479_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2804221.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28480..29502
                     /gene="splB"
                     /locus_tag="PC479_001674"
     CDS             28480..29502
                     /gene="splB"
                     /locus_tag="PC479_001674"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2804222.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29684..30595
                     /locus_tag="PC479_001675"
     CDS             29684..30595
                     /locus_tag="PC479_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2804223.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30610..31026
                     /locus_tag="PC479_001676"
     CDS             30610..31026
                     /locus_tag="PC479_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2804224.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31126..31518)
                     /locus_tag="PC479_001677"
     CDS             complement(31126..31518)
                     /locus_tag="PC479_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804225.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31659..32054
                     /locus_tag="PC479_001678"
     CDS             31659..32054
                     /locus_tag="PC479_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2804226.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13223 a         3418 c         5887 g         9680 t
ORIGIN      
        1 taaaaatatt ttcaaagagt ttattataaa attgtaaact atagtgttta taatctttgt
       61 aattattggt aattaaattt aaaaaataaa atatttaatt atacacaaaa aagatattta
      121 aaaaatttgt tatttattgt atacttaagt taaaactaaa taggcaaatc tagagaaatc
      181 tagtgacgca aagctatagg gactaaggtt tatatacata aactatgtca gccagttgcc
      241 aaaaagagtc ctaggtgtat tgtataccta agaaaagtct ataatgactg cctttttggc
      301 agtcattttg ttatggaaaa atcactttat ttcagtagaa aatacacata ataacatatt
      361 ttaccaatta taacataaaa taaagaaaaa ttatctcaaa aagttactat tttacaaaaa
      421 agagcaactt ttcgaagaaa tatttaaata catattaaaa tttttttata ttttcatggg
      481 acttcattaa aaaaatatta gttttcttac taaattgata cattattgta ttaatgtaat
      541 ggaaatgcga ttaatttaaa taaataaaga agtcattttt tgtaatatag caacttcatt
      601 ttttttatta ataaacaaaa tagataaaat atattttgag gaggggtgag tgtgaataca
      661 tatgacatta taaaaatggg attagatgca actaatctaa gaagtcagac aatagcaaat
      721 aatatagcaa atataaatac tgaaggatat aaaagacatt atataacttt tgaagaaaca
      781 ctaaatgatg ctatgcctaa aatagaagaa aaaacagaca attctaaagg tttaagagta
      841 gatggtaata atgtagacat agaagaagaa aaagtaaatc aagcaatggc aacattacaa
      901 tataatgctt tgataagttt tgcaaatgga aaaatagcaa tgaccaaatc aataataagt
      961 gggaggtaat tagaagatga gtgtgttcag tggaatgaga atatctgcta gtggattaac
     1021 agcagaaagt ttgaaaatgg atatcatatc atccaatgta gctaatataa atgctacaag
     1081 aggtccaaat ggagaagcat ataggagaaa agttgtttca tttgaagaaa actatgataa
     1141 taaattaggt atgctaggag taaaagttac atctgttgaa gatgataaat cacctcttaa
     1201 agaagtatat gacccatctc atccagatgc aaagccaaat ggatatgtag aatatccaaa
     1261 tgtaaatata ttggtagaaa tgacagattt aataacagct tcacgagcat atgattctaa
     1321 cgtagataca ttgaatgctc agaagtctat gatatcaaaa gctttggaaa taggaaagta
     1381 gggataaatt atgccaatta cacaaattaa caatttaatg caaggtgcaa agttagataa
     1441 tttgattgga gaagcaaagc cattaactct taataataaa gaagaaaaaa agaattttga
     1501 agatgtaata atagatacta tagataaagt taattttcaa caagtaacag cagatgatat
     1561 gaaagaaaaa tttataaaag gcgaagatgt ttctatgcat gaagttatgt taataggtca
     1621 agaagcacaa ctatctatgc aatatttaat agaagttaga aataagattc atgatgcata
     1681 tcaagaaatg aataaggtgc aaatttagcc ttttgaacag gagtaataat caatgataac
     1741 cagttttaaa aataagatta aggagcaagt tgctaaatct aggacaaaga taagttcttt
     1801 aaaaaaggga actaaaatag cattaggtat aggggttatt gctatatgtt tagcgctagt
     1861 atttacagca atatctgcga gcaaaaataa gtatgcagtc ttattttcag aactagatgc
     1921 tcaagatgct gcagatatat cagcagagct agagtctaaa aagatagata ctaaggtaaa
     1981 aggaagtacg atttatgtac ctagaacaga agttgataag ttaaggctgg agttagcgcc
     2041 aagtcttaca ggtggttcta aaggatttgc actaatggat gatagttcat caatagggct
     2101 tactgatgaa gaatttcaga ttaagaaaca aagaatgata caaggtgaaa tagaaaaaac
     2161 aataaagagt tttccacaag tggaagacac tagagtacat ataacatttg gagagtcatc
     2221 agtatttgag aaggaggctg ttccagggag tgcagcagtt tatttgatgc taaaaccagg
     2281 gacgacttta gaaaaaaacc aaataaagtc tgttatggca cttatatcag gaagcgttac
     2341 aaatatacca gagaaaaatg ttcaggttat tgataacaaa atgaatttat tatctgaagg
     2401 tattttggag gatgaaaatg ttgatgaaaa taatcaacca ataaatagtg gcattagtgt
     2461 tgataaaaat aaggcatctg aacaagcatt gaataaacaa ctagaaaagt ctattttaga
     2521 aatgctagaa ccgatatttg gtaagggaaa ggtgaaagct acagtaaatg ctgatttaag
     2581 ttttgataca gctgaaaaaa ctgagataaa aattgaccct gataaagttg caataaagga
     2641 gacaaggtcg aaaaattcat ctaaatctgc tgcaaataat gttcaaggtg tagatgctaa
     2701 catgaataac caaggaaata acaatggtac taatacagaa gatagtttag atgagagttt
     2761 tgagtatgaa acaggtaaga ttgaaactca cactatagtt gcgcctggag aacttaatag
     2821 attgacagct tctgttgcaa tagatggaaa aatagctaaa ggtgttcaag ctgatgtaga
     2881 ggatattgtt aataatgcta taggtatgga tggagcgagg ggagatactc ttagcgttgt
     2941 ttcaatggta tttgacccag aaggtaagaa agaggctaaa caagagctgg aagagattaa
     3001 aaaagaagga tttaaaaaga acataataaa agcagttatt ggagtagtag cagtaatagc
     3061 agttggagct attggatact taatatataa gaaaagaagt gaacgagaag atatggatta
     3121 tgaagatgaa gattatattt ctaaatctga ttctgttgat aggaaggata atcctctatt
     3181 aggagatgac ccagaactga cattagagga agctgttaaa atgtatgcag aagaaaagcc
     3241 agaccaagta acagagataa taaagacttg gttaaatgag taagggggat agaaagtgtc
     3301 cacggaaatt acaaatgtta atgcgtttga cttaagtgat ataaatgaaa gtgataagaa
     3361 tgagaaggta gaaggtgagg gtttaaaaaa agctgctgta ttattaatga cattaggccc
     3421 aaatgtgtct agtgatataa taaaaagctt gcctgataaa caagttcaaa aaataggttt
     3481 ggaaattgca aatatttctt caatagcttc aaaagaaaga agagaaatct taaaagagtt
     3541 tgtagaaata aatagggcaa aagattatgt tatagaaggt gggcttgaat atgcaagaga
     3601 gttattaagt aatgctttag gaaatgttaa agcaaacaaa ttacttgaag gtatatcaat
     3661 aaatacaagt acaaagcctt ttaaacatat tataaaacta gatgtaaaga agatattaga
     3721 aatacttagg aatgaaagtg cacaaactat agccgtttta cttgcacata tggaaccaga
     3781 aaatggagca gaagttttat catacttaga ttttgattta caaaatgaag tagctatgag
     3841 aataagctca atgggttcaa tatctccaga agtaataaag gtaattgaaa gttcattaga
     3901 agataaatta cattcaataa gtggtgatga ttttaatgat tatggagggg tgtctacctt
     3961 agttcaaata ttgattaatt ctgatagaaa aacagaaaag aatattttgg gaactattga
     4021 agaacaaaac ccggatttag cagctcaaat taaatctcat atatttgtct ttgaagatat
     4081 tgtcaagtta gatgatataa gcatacaaaa agtgttaaaa gaagtgacta tgaaagattt
     4141 agtatatgcc ttaaaaggtg tagatgatga gatttcagat gctatatata gaaatcaatc
     4201 ttctagagca gcagatgcac ttaaagaaga aatggaaatg gttggtgtta taaaggttac
     4261 acaagttgag gcttctcaac aaaagatagc taatatcgta agaaaacttg aaagagaagg
     4321 tgccataact attgtaagga attttggtgg tgactttgtt gaataataag attattaaaa
     4381 gtaaaaaagc attatataaa ggagtgctag atttaaccat aatagataaa gttagagtgg
     4441 gagaatcaaa tgtaaataaa gaaaatgatt gtagatttag ggatgaggta ataaaagaag
     4501 ctatagaatc tgctgagata agaaagaatg agatattaga agaagcaaaa atgaatgcac
     4561 aaaatataga aaagcaagca tatgaaaaag ggtatagtca aggtcaaaaa aatggatatg
     4621 aagatggata taaagagtca tatgatgaat atacccaaaa agctaaagat gaagcagatt
     4681 ttataaaaaa tcaggcagac ttaatactat tacaagctaa tgagaagatg actgagtaca
     4741 taaaagaaaa aaataaagag attgtaagac ttgctattaa tatggctagt tctgttctaa
     4801 aaaagcattt cgaagatgaa gaatctatga gtgatttgtt aaagcaaatt attcaagatt
     4861 atgaaggaag tagttcattt atagtaagat gtaattcttt ctataaagat aatatagaga
     4921 aagagtttga attattcaaa gaggaaacat cactaaaatc aagtgtattt gttattgcag
     4981 atgatagtat agataaaggc aatgctatta tccaaaagga aaatggaaga ataatagttg
     5041 gtattgattg tgctatggaa aaactaaaag aagaattgct tggtgaataa agtatgtata
     5101 aaactgatat atctatagat tttgacaaaa taaataagaa aatagaaaat ttagaactca
     5161 ctatatcaga aggtagagta aagaagataa taggacttac tgtagaagtt gaaggaataa
     5221 aagcttttgt tggggagtta tgcgttatat acaaccaagt taataaacca gtgaattgtg
     5281 aagtagtagg ttttaaagat aaagaagtca tattaatggc gttgggagaa ttaacattga
     5341 tagcacctgg ttgtaaagtt atttctaagg ggataccttt aagtgttatg tgtagtgata
     5401 atttactagg taaagtactt gatggtcttg gaaatcctat agacaactca gatgctgttt
     5461 taggagatag atacaacctg aacaatgaac ctcctgaccc aatgaagaga aaaaagatac
     5521 gaaatataat ggaaacaggt gtaagagcaa tagatgcatt tactacttgt ggtgaaggac
     5581 agagaatcgg catatttgca ggaagtggag taggtaagag tacgactcta ggtatgattg
     5641 ctaggaatgc aaaagcagat gtaaatgtaa ttgctcttat tggagaaaga ggaagagaag
     5701 tactagattt tattgataaa gatttaggtg aagagggaat gaaaaaatct gttgttgtgt
     5761 gtgcaacatc agataaagcc cctctagtta ggttaaaagg tgctcttact gctactgcaa
     5821 tagcagaata ttttagagac caaggcaaaa aagtaatact tatgatggat tcagtaacta
     5881 gatttgctat ggcacaaaga gaagttggtg tagcaatagg agagccacca gctcaaaaag
     5941 gatatacacc atctgtattt gcaatgttgc caaaacttat ggagagaaca gggacatcag
     6001 ataaaggttc tataactgca ttttatacag tattagttga tggagatgac tttaatgagc
     6061 caatagcaga tacaacaaga gggatacttg atggtcatat agttttatct agagacttgg
     6121 caaataagaa tcactatcct tctatagatg tattaaacag tttaagtagg cttatgaatg
     6181 aaattgcttc aaaagaagac ataaaaatag cgtcttttgc aagagacatg cttgctgagt
     6241 atagagaagc agaagatttg ataaatatag gtgcatatgc ttcaggtaca aacaaaaaaa
     6301 tagatgaagc aatctattat catgagcata ttattaattt tttaaaacaa ggaataaatg
     6361 aaaagagttc ttttaatgaa actataagta gtttaagaag aatctttgaa taaacaattt
     6421 aaaaaaatat ttgaataggt tgtgatggaa ttggataaaa attttaagtt tagattacag
     6481 aaagttttag acttaaaaat aaaagatgaa gaagagataa aaatggaatt tgctaagatt
     6541 caacaaaaaa aaatagatat agaaactaat ttagaaaact tagaaagtaa ttacagcaaa
     6601 tattctataa gtaaaaataa tgataatgtt caaaatcaaa aaataactat taactatttg
     6661 ctagctttaa acaactctat aatggattta tcagaagaac tggataagtc gacaaatgaa
     6721 cttgaaaaag ctagaaaaca gcttataagc aaacagatag agagaaagtc attagaaaaa
     6781 ttaaaagaaa aaaaatatgg acagtattat aaagaagaac agttaaaaga acaaagcact
     6841 aatgatgagt ttgcaagcat gagttatttg agaaatagac aagttctata aaataatagg
     6901 aagtgaggat attattgtat gaagatacaa gcagattctt ataatattat taaagatatt
     6961 gggaatagaa aaaactcatc taagaataaa aatataaaaa ataataaatt tgaagataat
     7021 ctatataaga caacagacca taaaaatgtt aaagatagaa gagaaacaag taagcaaaat
     7081 gttagttcag atagaccggg aaatagtgat aatgagtgtc atttagatga agataaaagt
     7141 aatcacttag aagatgaaaa tacatatgaa aaagattgta gtaagaataa tgaagcttta
     7201 cagcaattac tgaatctttt aaaagagaat tataaaaaag ataaagatat aaacactata
     7261 ttgaataata taaaaaaact aagtatatca gatgaattaa aacaagaact taagcttaat
     7321 aaaaatatat tgaataaaat tgcaaatcaa aatttagagt taaatggagc taatacaaat
     7381 aaccctaatg atatagaaaa tttagacata atgaagttaa gagaactttt agaaaataat
     7441 gctagtgaag atattcaagt aattagtaaa actacagaaa atgatactaa aagcatagac
     7501 ttatacagtt ttgatagtaa tagaatggat actcttaact caaataaaca gaaagatgat
     7561 agttctgata ttttggaaaa actagcaggg ttaaatggaa ataagagtgg aaattttgga
     7621 caagtaataa ataaaagcgt tgatatgaat aaaattaaaa atgataatgt taatttaaaa
     7681 cagatagaat ctaatgtaat ggatgattct ataaaagcta ttaagcatat gaaaacaaat
     7741 gatatacaag aattgacaat taaattaaga ccaaaagaat taggagatat gaacatacaa
     7801 ttattaaaag atggtgaaag tatgagagct gttgtgactg ttttcaataa agatgtcttt
     7861 gactctataa ataaaaacat agcagattta aagcaacatc ttgaacttac aaatgtaaat
     7921 ataaaagatg tatcagtgca aatgcatagc gataatacaa atacatctga tacctttgat
     7981 aaagcatttg aacaacagaa tagacaaaat aaccaagaaa acatgagtaa tcaaaataca
     8041 aatgataagc gtaacaatgt agtcatagaa gatgagatta aagaaaatat tattgatgat
     8101 gatagagtag atttactagc ttagtaaggg ggaagtattg catgtacgga atagcaacgg
     8161 ctttggcagg aactcaggta aactataggt caaataactt aagccaactt gaaagaaata
     8221 ttaatgatat tgccaacaaa caagttagaa gtaaaggaaa tcagacagta acagacaatg
     8281 ggactccaat tataggacaa ggtgatgatg ctgataaaga cttgtttatg aagattcttg
     8341 tagcacagat gggtaatcaa gacccaatga atccacaaga cccaacggag tatgtatcac
     8401 aattagcaca gtttgcaact ttagaaaaaa tgtcagctat gaatgataag ctagatatac
     8461 ttttagcctt aagtaactct actctaataa actctgcttt gagtatggca acatcattaa
     8521 ttggtaaaga agtagaagta tctggggaag aagatgaaga tgaaaacatt gtaggagtag
     8581 taaagtccac atacataaaa gatggggaag tacacttaga attagaagta gatggaagtg
     8641 acaaaactgt agatgttaaa tatgaaaaat tacttaaaat aggagaggta gaggcaaatt
     8701 caggagaaga aaatgactct tcagaacaag aatctccaga agaagtataa tattaaatgg
     8761 gaaaagaggt aaatttatga taaaagcaat gtattcaggt gtaagtggaa tgaaagcaaa
     8821 tcaaacaaaa ctagatgtaa tcggtaacaa tgtagccaac gctggaacta cttcttttaa
     8881 gaaaagttca gctagaataa cagattcatt ttatcaaact gtactatatg caagtgctcc
     8941 aacagcggca ttagggggaa caaacttagg acaagtaggt gtaggtagta agatatcaag
     9001 tataaacaaa gatatgacac aaggaaatgt acaaccaact aatagaccat ctgatttaat
     9061 gatagatgga gatggatatc ttccagtagt aagacaagga acagttatgt atacgagaga
     9121 tggttctttt aatttagata caggatttaa agcagctgat ggtgctttga agaatattaa
     9181 aggtggaaga cttgtaaatg gtgatggata tatgttacaa ggtatgattt atgatgggga
     9241 atataaagct gatactggag aatttgttaa aaagggtgat gctaaaggaa aagagcctat
     9301 tgtaatacca cttcaaagta taagtcctaa ggatggaaca gttcaaaatg tactagagta
     9361 taatatatca aaggatggaa cagtagaatt tttattgagt gatggtcaaa gaactcaatt
     9421 tgtctatgat atacctgatg gtgctaaggt aggagataca cctgctggaa tagataaatt
     9481 aaaaggtaca atacaaaaag tgcaaacata tgcattccaa aatccagcag gactagatgc
     9541 agaaggagga aacttgttca agccatcagc aaactcagga gacccacaaa tagcaggagc
     9601 tacaggcaca ataatacaag gagctataga agcttccaat gtagatatag cagaagaatt
     9661 tacagaaatg ataatagcaa gtaggtcttt ccaagcaaac agtaaaataa tatcgacatc
     9721 agacgaaata cttcaagaaa taataaatct taagagatag taggtacata ttatgataag
     9781 agtaacggat ttaaacggaa gaaaatacat attaaattca gatcttatag tgagaattga
     9841 ctccgtgcca gaaagcagga ttctccttac aacaggagaa aaaaatcttg taaaagagtc
     9901 agtagatgag atagtagata aagtaataaa gtataaaaga aaaataaatg taggttatat
     9961 taggagtgaa aaataatgaa aaaaacagat tcactaacat tcattggact aatattatct
    10021 acagtattag ttttatttgg agcagtaaaa ggaagtagtt caggattgaa aagttttttc
    10081 gattttgcat ctatattaat cacagtattt gggtcatttg gagctttgat gattacattt
    10141 actattgacg atataaaact tataaaagat gctctacaat actcattcaa gacaatgaat
    10201 atatcgaaat tagacttatt aaatcaattt aaaacacttt ctaaaaaagc aagaagagaa
    10261 ggacttttat ctatagaaag tgatgtaatg aaaatacaag acccatttat gaaaaaagga
    10321 ttagagttat gtattgatgg tcttgaaata aatgaaataa ggtctatact agagctcgaa
    10381 atagattctg ttgaagaaac caataacaga tattctaaac tatttaaaac ttgggggact
    10441 ttcgcacctg catttggtat gttgggaact ctaataggtt taatacaaat gttatccgac
    10501 cttacatctc cagatttaat agcatctggt atgggaaaag cattaattac gacattttat
    10561 gggtctttac ttgccaatat cgctttgaac ccaattgcat ataatataga tgagaagact
    10621 gaaaaagaaa tttatgtaaa ggaaatgatg ctggagggga taataagcat acaatctgga
    10681 gagagcagca tagtagttga ggaacgttta gctacatacc tttcaaataa tgagaagtta
    10741 gaaattatga aatccaataa aaatacagaa agagcaatga gtaatggcgc gtaaaaaaaa
    10801 taagaaaaag gatgagataa atccagatgc atggttggct acttatgcag atacgataac
    10861 actgattctt acattttttg tgttattgta tgctacatca gttgtagata tggaaaaatt
    10921 taaaaatata gcagatgcgt taaaaggtca gttttctggg acaagtatgt ttagtacagg
    10981 ttcaagtaca agtgagaaat ctccaataga gagtgtttta gtggataaaa atgataagtc
    11041 aaatttagaa caggatttaa aagataaagt taataattct aacttacaaa atagtgtaac
    11101 agtcaaagat gatagtagag ggattttact agagctagat gattcaattt tatttgactc
    11161 tgggatttct gaattaaaag aagatagtaa agccactctt aataaagtat acgatatgat
    11221 aaagatgatg aaaaataaga tagttataga aggccatact gataatgtac ctgtaaaatc
    11281 aagtacgcat gaatccaatt gggatttatc ttctagtaga gcagtaagtg ttgtaagata
    11341 ctttgtagaa gaaaagggca tggaccctaa actattttcg gcaacaggat gtggagagta
    11401 ccaacctttg gtatcaaatg aaacagcaaa gggaagagct gagaatagaa gagttaatat
    11461 acttataact attgactagg aggaagaggt ttgttcaaaa ataaaaaaat tattataatt
    11521 attttgatac tgattatttt ctcagcagga gcaggttttt ttatattagg agaaggtgga
    11581 aataaacaaa ttgacaagat tgcaggtttg tttattccaa cagggaaagg caaagatgaa
    11641 gtaaagaccg aaaatcttga cttagaagag atggtattga agctagctga tgaaggtgta
    11701 agatatttaa aagtatcaat aactgtatct tatgattctt catataaaga tatagaaaaa
    11761 aatacgcctt taattaggga taatattata aattgtttta tggcaaaaag ggcagatgat
    11821 tttactgctg ataatttaaa gaacataaaa gaagaaatta aaaatacatt aaatatacaa
    11881 ctaggtgaaa atgtaattca aaatatatat ttcacaaata tagtagtgca ataaaaggat
    11941 ggtaagacat gatactttat ttactaaaaa tattaatatg tatcccaata attttaatat
    12001 tgatagtctt atctttaaaa attagccaag gaactttatt aaaaaaatat aatgataaat
    12061 atataaaaat actagatatt gtaaatataa gtaaaaacaa tagcataata attttaaaaa
    12121 taggtgaaga aggatgcatt atggcatctt cttcttctag catgagtaaa ataaaagatt
    12181 tgaccaaaga agaaatatta agaatagaaa acgacattaa agaaagaaat tttaaaattc
    12241 aagattttaa aatggaaaga attaaaaaga aatatgattt cagtagatat attggaaaat
    12301 ttacaaaaaa ggaagataag taaatggaac tattatcaaa attacctttg gctgatgtac
    12361 catatacaag ttcaatgcaa ttgtttttat ttctaactgc attaatgttt ttaccatttg
    12421 taatgttcat gatgacaagc tttgtcagaa tagttataag tttatcattt ttgaaatcag
    12481 cattaggagc acagcaagca attccaagtc aaattttggt agggcttgca atagtattga
    12541 caatatttat aatgagacca gtattaaatg agataaacga aaaggcatta caaccatata
    12601 tgaaagaaga agttactatg gaagaagcta tgaaggaagc agaaggacct attaaagaat
    12661 ttttattaaa gcaaaccaga cagacagatt tagatttatt tgtagagcaa gctggtttaa
    12721 aagaaaagaa attaactcgt gaaaatatac cattatctgt tgtagttcca gcatttgcta
    12781 taagtgaact aaaaactgct tttcaaatag gttttttaat atatatacca tttttgataa
    12841 tagaccttgt tgtggcaagt gttttgatgt ctatgggtat gtttatgtta cctccagtta
    12901 tgatatcatt accatttaaa ttattattat ttgtaatggt ggatggatgg aacttgatag
    12961 taaaaacatt gatattggga tttggatagg agaaaaatag tatgagtgaa tttatgataa
    13021 tgagtgttct taaaaatggc atgtttacag gagctatgat tgcagctcca gtgctaattg
    13081 tttctcttgt agtggggctt ttaataagtg tatttcaagc cacaactcaa atccaagagc
    13141 aaactttgac ttttgtacca aaactaatag caattctttt agtagtagct attggtggaa
    13201 gctggatgtt acatactctt attagcttta cttcggagat attcaatatg atgtctctca
    13261 taactaaata agtaggatat atatgatttt tgtatttatt agaatagtag cattctttgg
    13321 aacattaagg atactatttc cagctggtac acctgcttta tttaagtcgc tctttgcaat
    13381 cattatatca atcttgattt caagtactat gcaaattgaa tatgcaacaa atatagacaa
    13441 cattgtattg tttaccttat atggtataaa tgagaccata actgggatta tgcttggata
    13501 tataaccaat ctatgttttt atagtatcag aatggctggt tctatgatgg accaacagat
    13561 gggattatca atgattaata tgtttgaccc aaattcaatg actcagacaa ctttaatcaa
    13621 taatcttatg aactggacag cacttatgat attttttagc atggatggtc atcatgtttt
    13681 aattagaggc ataaggtata gttttgagtt aattccaata gggaaatcat tcatagataa
    13741 taatatagat tatataataa acatatttgt gcagtgcttt ttaactggat tcaaaatagc
    13801 aatacctatt gtactttgtc tgcttatggc agattttata ttagggctta tatcaagaag
    13861 tgtacctcaa ctaaatgtaa tgataattgg tatgccatta aaaatactgg ttggtattgc
    13921 attatttatc atatcaatac ctttaattgt caatcaaata agtcatttat taagtcaaat
    13981 accaaagatg tatgagggaa cttttgcact agctcctatg ttttttatgg gttcaacaga
    14041 taaaacagaa gaagctactc caaagaaaaa gggagaacaa agaaaaaaag gtaatatagc
    14101 aaagagtaaa gaattaccca ttgcaatgac tctacttgca ttcactttat tagtacctac
    14161 attattttca tatgtagtga atacacttaa atcttctctt aattattttt tgagtctaga
    14221 tttttatatg aacataaatt attcaagtat agagaaatta gttatagcag gattaatgga
    14281 tttttttaag atatttttac caatagctat tccattctta gttttaggta tagtcgcaaa
    14341 cctatttcaa gtaggaatac tgtttactgg tgaaacttta aaacccaatt tatcaaaatt
    14401 aaatccaatt agtggattca aaaacatgtt ttctatgaga agtttgtcaa ccttaataaa
    14461 agatacagcg attatatcta ttttggctta cataggatat acattcttcc aagataatta
    14521 tttagatata ttaaagttag gtaacatata cctgccaact ttaatgtaca cagttaaaga
    14581 tttagtatat tcaatattaa gtaagatttg tgtagctatg atagttatag cagttgcaga
    14641 ttatgtttat caaagataca gtcataaaaa gcaacttaga atgacaaaac aagaagttaa
    14701 agatgaatat aaaaactcag aaggagaccc agaaataaaa gctaagataa aacaaaaaca
    14761 aagacaaatt tcgtctcaaa gaaccatgca agcagtacca agtgctactg taatagtaac
    14821 caatcccacc cacttatcta tagcagtgag atatgaaaaa gggaaagacc aagctccagt
    14881 agtggtggca aaaggtgcag attatttagc ttttaaaata agggaaattg caaagggaaa
    14941 tgacatacct ataatagaaa ataaacctat tgctaggttg ttatataagc aagtagaaat
    15001 agaccaagaa attccagagg acatgtatca agcttttgca gagatattag tagcagtata
    15061 taaaatcaaa aatagataca aagtccccaa aaggtaggat aagtaatgaa taagtttaat
    15121 ttaaaggaaa atatggacgt aatagtggca tttggtgttg tagggattgt tcttatgatg
    15181 atagtgccgt tacctacatt tatattagat attttactgg caattaacat aagtttatct
    15241 gtgctaattt tactaatgac actatttaca acaaatgtac ttgatttatc tatatttcca
    15301 acagtgttac ttgtgacaac actatttaga ttgggattaa atctttcatc aactagattg
    15361 attttaacac agggatatgc aggtgaagtt atagaggcat ttggaagttt tgttgtaggt
    15421 ggaaattacg tagtaggaat aattatattc ttaattatat taattgttca atttgttgtt
    15481 ataaccaatg gttctggaag agtatctgaa gtaacagcta gatttacgtt agatgccatg
    15541 ccaggtaagc agatgagtat agatgcagat ttaaatgcag gagttataga tgacaaaact
    15601 gctagaaaaa gaagaaaaga gcttcagcaa gaagcagatt tttatggctc tatggatgga
    15661 gcaaataagt ttgtaaaggg agatgccata gcaggtctta tagtaacagc aataaacata
    15721 ataggtggaa ttgtaatagg agctgtaatg ctgaatatga ctcttatggg tgcagcacag
    15781 acatatgtaa gacttactat aggggatggt cttgtaagcc agatacctgc tctattaata
    15841 tctacatcag caggtataat cgtaacaaga gcttctactg atgaaaactt tggtggatta
    15901 gtaggtaaac agttaacagc aataccaaaa gcagtaatga tgactggctg tgttctaatc
    15961 gtacttggta tgatgcctgg attacctaaa ccagcatttt tttcactagg tataggtgct
    16021 atagcagcag gttacttttt aggaagagaa aagaaagata ctttagaaga cttggagttt
    16081 gaaaaacctg atatggtaaa tccagataaa aatgaagtag aagatgtaac aaacttaata
    16141 aatgtagaat caatagaagt agaaattgga tatggtttaa tatctttagc tgatgaatca
    16201 gcaggaggag atttacttca gagaatagca tcaataagaa gacaatgtgc aatagacatg
    16261 ggtatagtcg tacaacctat aagaataaga gataatttac agttaaatcc aaaccaatac
    16321 aatataaaaa taaaagggaa tgtagttggt gggtatgaga taatgcctaa tatggtttta
    16381 tgtatggatc ctatgaatca aggatttagt atatttggaa taaagacaat tgagcctact
    16441 tttggaatag aggcactttg gatagaaagt gacaaggtag aagatgcaga gctaaagggt
    16501 tatacagttg tagattcatc cacaatactt ataactcact tactagatat aataaaatca
    16561 aatgcacacg aattactggg aagacaagaa gttaagacta ttatagatgc agcaagagaa
    16621 aattatagtg ctgtagtaga tgaattgata ccagatttaa tgacacttgg tgaaattcaa
    16681 aaagtactta aaaatctgtt aagagaaaaa gtaaatataa aggatagagt tacaatactt
    16741 gagacacttg cagaccagtc tagaaataca aaagatatag agcttcttac tgaatatgta
    16801 agaatagctt tggctagaag tatttgtaat aatttagttg atgaagataa ggcaattgta
    16861 gtggctacat tgtctccaga aactgaagaa ttggtatcta ataatttaca aaggtctgta
    16921 aatggaactt atccagcagt tgacccagaa aatacaaata aaatatttga gagtatacaa
    16981 gaagtaatga acaatgtata tttcaatcat aatataccag taatagtagt atcaccaaag
    17041 ataagagctc catttagaaa attagttgaa attgtatatc ctaatttgac tgtattatca
    17101 ttaaatgaaa tacctaacga tattaaaatc aaagcagaag gtgtggtgag catatatgat
    17161 gacgaaaaag tatacagcta acacaattca agatgctatg aatctagcca aaatggaatt
    17221 gggtgacaat attacattaa tagataaaaa agaggtaaga aaatctggaa taagaggcat
    17281 tttttcaaag aaagatattg aattgactat tggatgggaa aagaaagata atttagaaca
    17341 aaaagattta aaacgagaga tagaacaact aaagtcaatt ataaataata tggggtttga
    17401 taataaaaat gataatgata tagataaaat atgcaagaat ttactaaact tagatttaaa
    17461 tcaagaaatt gtagaattta taaaagctga tttacaagaa atgaagttca atggaattga
    17521 tacaagtaaa aatttagtag aaagtctaaa gaaaaagatt aaaatagaaa atcaagcaat
    17581 aaatggtaaa attgctttag ttggaccacc aggagtaggt aaaacgacta caatagcaaa
    17641 gttggctgcc aaattagttt ttgaggaaaa taaaaaagta ggagtaataa ctatagatac
    17701 atacaggatt ggtgctgtag aacagttaaa gatatatact gatataatga atattccttt
    17761 taaaggggta ataagccctg atgagatgga attggcactt gatgagatga aagattgtga
    17821 tgttgtatta atagatacaa caggtagagg ctataaaaac tctatgcaaa tattagaaat
    17881 caaaaatctt atagataaag ctgaaactga taatatacat ttagttttga actgtacaac
    17941 aagagaaagt gatacaaaag caattataga ttcatataga aatgtgaatt ttaaaagctt
    18001 aataattaca aagttagatg aaacaataac atatggctca atatttaaca taatgaatta
    18061 tgcacaaaaa ccaatatcat atattacaac aggacaaaat gttcctgatg atattataaa
    18121 acctaatgaa gataagatta taagattact attaggagtt gaaagtatat gatagaccaa
    18181 gctcaaattt tgagaaaaat ggcaatggaa aaaagaggtt ctgatgagat tatagtagag
    18241 aatgagaata tgcctaagat aattacaata gcttctggta aaggcggcgt tggaaagagt
    18301 aatctcgcta caaatttgtc tatatgctta accaaacttg ataaaaaagt tcttatatta
    18361 gatgcagata taggaatgtc taatatagat ataataatgg gggtaaatgt aaaagggact
    18421 ataattgatg taattaatgg cgaaaaaaat atagaagata taatttcaca gactaagtat
    18481 ggtgtaaata taatatctgg aggttctgcc cttaatcata tagaagactt tacagaagct
    18541 caaagaaata agtttataca tagcatagaa cagattcaca atgtggattt tataataatt
    18601 gatacaggag ctggtatgag caaaagccta ttgtcattta tatactgtag tactgaattt
    18661 ttcttaataa caactccaga accgacatca ttgactgacg catatagttt attaaaagct
    18721 ataagtaatt ttggcataaa gaaaagtgca aatataataa tcaatagggt aattgattta
    18781 gaagaagcga aatctacata caaaagaata aaaatggttg tggacaaatt tctaaacctt
    18841 aatctggaat tgtatggata tataatagat gacaaaaaag taggtgtatg tgtaaaaaaa
    18901 cagataccat tcattttaga atatcctaca agtattgcat caaaatgtat tatagctata
    18961 gcaaagagaa tggtaagtca gtcaagagaa gatattgttg ataataaaag ttttctaaga
    19021 aagatgttta gtatatttaa aagctaagga ggcgtagtta atgaatagag aagaattaat
    19081 aaaagagaat atgcctcttg taaagagtat agcaagaaaa ttttttatac cagggaaagg
    19141 ttttgaatat caagacttag taaatagtgg agtcattgga ttgattgatg caataaataa
    19201 atttgatact gaaaaaggag caaagttttc tagttactct tatataaaga taaaatctgc
    19261 tatattagat gaaattagaa atcaaagtcc aatttctaaa cataatctaa ctaaagtaaa
    19321 taagtataat agagtggtag aaaagttgca atctaaatta ttaagagagc caacttcata
    19381 tgaaattgca aaagaattaa aggtttcaga aaaagaactt catgatatcg aaagtaatat
    19441 agatatgtta aatattgtat cattaaacta tgtaatcttt gaagatacaa atgaaactgt
    19501 acaagatgta ataagtgata gagaagagga agctccagaa aatataatag aagaagaaga
    19561 aaagttagag attctatcca aagcaattag taatttaaat gaaagagaaa aattaatact
    19621 ttcactgtac tattatgaag atttaaatct aaaggagata gggaaagtgc taggtgtttc
    19681 agaatcaaga gtttcacaac tccacagaaa atcaataaga aacttaagaa acaagataaa
    19741 agaacttaaa tattctatat agatggtgat tgataagatg atacaaatta tattaatagt
    19801 agtgtctatt ttaataatag taactatagt tataaattta cagaaggaat caaaaaataa
    19861 agataacaga tactttgata aaatatttga gcaagaatat gaagcaaatg atagattaat
    19921 aattgcagaa aaaagaagat tctttttaga caaagtactt gcagaaaaaa gaattaatag
    19981 tcatataaaa gtttatgaaa attataacct aataaaggct aatgaagaaa gaaaggaaaa
    20041 catatttata gaatcaaaaa aggaagtaag acaagaaaat gagttatctc ttaaagtgaa
    20101 acatcttata agttcaggtt atgatgatgt ggaaatatgt aagatgctag atattggaaa
    20161 gggggaactg ttattaataa ggagttgtta caaaatataa ggatattatt atgtgataaa
    20221 aaagtattct taggaatagg tattggtata ttaatatcaa ctttatgtat aatgcttttt
    20281 agtaataatg atatgagtaa ggcagagata gaaaaaaagg ccagagcgtt aggcatgaaa
    20341 tatacaagtg agatgaaagt tttagaatag atgatactgt ttagacattt acaaaaaagg
    20401 aggtcataat gtgataagag gattatatac agcagtttct gctatgataa ctaatcaaca
    20461 aaagcaaaat gttgttgtca ataacctagc taatatggat acaaatggat ataaaagtaa
    20521 acaacttttg actaaaagtt ttgatgaatt aaagttggag ggttacaata actacgcaaa
    20581 tggaactaaa caaaagcaga ttataggcga tataagtcca ggtatttcta tggatgaaac
    20641 tatcacaaat tttaatcaag gacctataaa aactactgat aataaaatgg atgtagctat
    20701 tcaaggtaag ggattttttc tagttagtga tgcagctgga aatcaatttt acactagaaa
    20761 tgggaacttc agaacagaca atcaaggtga ccttataaca agtgaagggt attatgtact
    20821 tgggacgaat actgctactg gtgctgtagg tcatataaat gtaggaaatc aaaaatttga
    20881 agtggcaaaa gataatacaa tatcactaga cggaaatgct atgtataaat ttaatattgt
    20941 agattttaat aactacaata acttaaaaaa agaaggagat aacttatatt ctggaggagg
    21001 agctatgaaa gtaaataatg cacttacaat acaaagtgcg atagagtcct ctaatgtaga
    21061 tatggtatct gaaattaata atatgatgac aatatcaaga gaatatgagg caaatcaaaa
    21121 gataattcag gctatggatt ctaaattagc aaaaatagca agtgagattg gttcagtaag
    21181 ataggtaata ggaggtgaat ttttatgtac acaatgtttt atactagtaa aacagcttta
    21241 tcagcaaatc aaagtaaact agatataata tctaacaata tagcaaattc tgattcaaca
    21301 ggatataaaa aaataaatat ggaatattct gatttagtgg atgaagtact aaatagacca
    21361 tcatacccta caaatggtaa agatatatcg actggaacag gagctaaggc aacagctccc
    21421 atgagaaatt atgaacaagg tgcattggct cctacagata gtaagagtaa tttggcgata
    21481 gatggagaag gattctttag agtgataaga aaagatggta catatgcata tacaagaaat
    21541 ggtggattta atgtagatgc tttaggtaag attgtagatg ataatggaaa tatattagat
    21601 gtccaatttg atgcaggata taactataat aatacaaata tagatagtac aaacctgaca
    21661 gtttcaagaa atggagagct attcgtagat aataaaaaaa taggaaaaat taacttatat
    21721 caacctatag gaactcaaaa ttttatatct gaaggagata gtttatttgt cgcaactgat
    21781 gcagcacaga ttaaacaagt tgagaaagtc gatatagttc aaggctatag agagaggtca
    21841 aatgtttctt tacaagaaga atttgttgac ttaatagcca ctcaaagagc atttcaaatg
    21901 aactcaaagg gaataaagac agcagatgaa atgtggcaaa tagcgaataa tttaagagct
    21961 aaataattat gtaaggagag gaatattcat gaatgatatg aaagagtacg attttggcaa
    22021 acctcagagt tattcaagag aaaatttaac ttgtttaaat tttttattag ctgaattttg
    22081 taaaaaatat aaaaactata taatttatga acttaagtgt aattctagta tggttgttga
    22141 tagtatagac caagtaaatt atcagacttt tttagataga gtaacttctt catgcgtagt
    22201 agtacaaaat gcaatggaac ctgttatgaa aaacttttct tttagaatag atagaagtgt
    22261 agctgatatg tggattgata taacttctgg aggaactgga gttgtaaagg ataatgatag
    22321 agagcttaca gaacttgata aaaaagtgct tttacacctt atggatgatt tagtaagaaa
    22381 tatgcactta tttgagggtt ttgaaaatat acaagtattg gatacacata cacatattaa
    22441 tttgccacaa ttatgttctc caactgcacc agtatgcgtt gtagatttaa aagtgctaaa
    22501 tgataatgag tgtattggaa gtgtttctct ttgttttcca tacaatggtt tagaggctct
    22561 gttagaaagt atatctgtta tagaattttt tgataatgat gtaggagatg attcagagga
    22621 atttacccaa aaaatctata atagtgtatc taatatagaa ttatcagtta ttgcagagat
    22681 aggaaaagta tcaattaatg ttgaggattt acttaaacta gaagttggag atgtaatagt
    22741 aacaaataaa aaaataaatg attatataga tatatttgta gaaaattcaa aatcatacac
    22801 agcaacacca ggatttatat caagtaaaaa aggtgtaaaa ataaatgatg ctgttggaaa
    22861 agaggtgtag ttatggacaa tagtaatctt atttcagatg aagaagcaaa gatgttatta
    22921 aatgataaag ttgaaagtga agttgatgga gatgatgagt tagccttaga tgtctctgaa
    22981 gttaatcgaa ataatataca aaggatactt gaccttaagt tagaattatc tgtagttata
    23041 ggaagaacta agatgactct aagagatatt ttaaatttac ataaaggttc tcttattgaa
    23101 ctggatactt tagcagacca agatgtggaa atactaatag ataataaggt tttggcttat
    23161 ggaaaagtag tggtggtaga tttaaatttt ggtgtaaaga taacaagtat agtaagtgaa
    23221 gaagacatgg taaaatctct tgtttagaga taacatcaat taatacttaa ggcatgaggt
    23281 ggtacttatt ttaaacttaa atgatataaa aaaagatata ctaaaactca ataaaaaaga
    23341 ctcatgttct ctatataatg aatctattaa taatataaat aataattgta tatcaactgg
    23401 attaaaattt ttaaaacaag caagagattt aaacccagat gatgttgata tattaaatct
    23461 cacaggtctt gtaaacttac taaaatgtaa ttttgatgat gctgtagaaa gtttctataa
    23521 gagttcactt tgtgaaaaaa ctgctctgtg tgaaaagtat ataaacatgc ttacatctga
    23581 ggagttttta attttctttg aaaagtacaa tcaacttata aggttaataa ataaaggaat
    23641 gtataaagaa gctatacaag ggtttaaact cataactgac caatactgtg atttaataga
    23701 accttatgag ttgcttgcct tactgtatga caaggaagaa gaatatataa aatttgatga
    23761 atgtttagaa gtattgaaga ttattgataa ggaaaattat ttattaaatc aagaaaataa
    23821 cacattagat atgtataata gtgctaatat acaaactaaa agtaatataa aagtagatga
    23881 ttataagaat gagttcttac ataaaagtag tagtaaccat aaaattaatg attataatga
    23941 tgtaactaaa gaaaagagtt caagcaaaaa ccagaaaagt tcagattcta ataagaaaaa
    24001 aagtattaaa aaaaataagt acctatatat agccataata ttaggaatat tattagtagg
    24061 acaaagtata tataattctt ataagatgaa taatttaact gataaaatat ctaaaaataa
    24121 tgaaactaaa aatgaagagt caatagattc taacttgact aagaaagaaa ataatataga
    24181 agaaaatgaa gatatgagta aggcttctaa tataaataag agtaataaaa acattaataa
    24241 agaggatgat gtaaaatcta aaaaaaatac tgtagaaaat gaagatgata aagattctga
    24301 cttatttaca gaggatgaat tgatgtctaa ggcgaatact ttaaaatcag aaaagaaaac
    24361 taaatcttct atacaactat ataagaaagt tgcagatata ggtgaaaata aagggaatac
    24421 atctgaagca acttatcaag ttgctattct cagtgaaaag ttaaaagact ataaaactgc
    24481 tgaagaatat tataaaatgt atgtagaaaa ttactcagaa aaagatgctt attttgatga
    24541 atcttattat aatttaggta tgatgtacta taacaatggt gatttaaaaa attctaaatt
    24601 gacattaaag aagttggtta ataaggtacc aaatagtatg tataataatt caaaagtaaa
    24661 agaaatttta aaagaagaat agttaaatta gataagaaaa ataaattatt ttattttaaa
    24721 ataatatgat tataatatat tgacatacca ttttaaaaaa tttaaaatat taattataaa
    24781 atgaggaggg ataaaaaatg gaatttgaaa aagggagtat ttcaattcat acagaaaata
    24841 tttttcctat aattaaaaaa tggttatact ctgataaaga catatttata agagagctta
    24901 taagtaatgg atgtgatgca gtaagtaaac ataaaagact tgtatcacta ggagaaatat
    24961 cagaaaataa gtcatcagat tataaaatta cagtatctgt aaataaagga gaaggtacac
    25021 ttaaatttat tgacaatgga attggtatga ctgaagaaga gataaaaaaa tacataaatc
    25081 aagttgcttt ttcaggtgct gaagatttct ttaataaata taaggataaa atggaagagt
    25141 caaatgatat aataggtcat tttggattag gattttattc tgcatttatg gtgtctaaaa
    25201 aggtacaaat agatacactt tcatatacag aaggagcgac tcctgtaaga tggatatctg
    25261 aaggtggtac agaatacgaa atatctgaat cagatgctag aaatgataga ggaactacta
    25321 taacactatt tattgatgat gatagtaaag aatttttaga tgaatttact gtaagaggaa
    25381 taataaataa atactgttct ttcttaccag tagaaatata tttagaagat gttgaaagat
    25441 tagaaagaga agctaaagaa gcagaagaaa aagctaaaaa acaaaaggaa gatggaaaag
    25501 aagagattgt agatgcaaaa gtaattgagc ctttaaacga cactaatcct ctatggttaa
    25561 aatctccaaa agactgtact gatgaggagt ataaagagtt ttatagaaag gtattcaatg
    25621 tatttgatga acctttattt tggatacatt taaatgtaga ttatcctttc aatttaaagg
    25681 gtatacttta cttccctaaa ttaaaaaatg agtttgaact gactgaagga aaagtaaaat
    25741 tatacaacaa tcaagtgttt gtggctgaca atataaaaga agttatacca gaatttttac
    25801 ttttattaaa aggagttata gactgcccag acttaccatt aaatgtatca agaagtttct
    25861 tacaaaatga cagggatgta agcaaaattt ctaaacatat aattaaaaaa gtagctgata
    25921 agttaaaatc tctttgtaaa aatgaaagag aagaatacaa taagttttgg gatgatatac
    25981 aaatatttat taaatatggt tgcttaaaag atgaaagttt ctatgagaag gtaaaagaat
    26041 gtatattatt caaaactatt gatgatgagt atataacatt acaagattat ttagaaaagt
    26101 gtaaggacaa acatgaaaat aaggtatttt atgtaagtga taaagaacag caatctcaat
    26161 acattaaact atttaaagaa tacgacttaa gtgctgttgt tttaaactca tcaatagata
    26221 ctcattttat ttcatttatg gaatataaag agaatggagt taagtttaat agaatagatg
    26281 cagacctttc tgatgtatta aaagataaaa atgaaaataa agatagtgaa gaaaacaaag
    26341 aagagatagc taagatagaa ggtctgttta aagaagctgt tggagaaaga gtgaagaatt
    26401 attctgtaga aggattgaaa aatgaagaca ctccagctat ggtgttagta tcagaacaat
    26461 ctattagaat ggcagaaatg cagtcaagat ttgctggaat ggatttagga atgaattttg
    26521 aagaagaaaa aactcttgta ataaatgaga acagtccaat aatcaaaaaa ttggtttctc
    26581 ttaaagatga tgaggaaaag aaagataaga ttacgcttat ttgtaaccag atagcagatt
    26641 tagctttact ttctaataaa gaattaaagc cagatgagtt agattcattt gtacaaagaa
    26701 gtaataagct tatgagtctt ttgatagaat tataagattg aatttgaata cattgaagca
    26761 aatttataaa aagtattcaa tatcagtgat aagaaagaag tgtctcaaaa tgaacttttc
    26821 agttcatgag gcgctttttt tatgtgaaat taaatttatt ttaatcattt caacaatgaa
    26881 aaatatattt atctctattt tgagacagac cgttctttat ttttttatct ttggttaatt
    26941 tttaaatatt taagtaggtt ctaataaaaa actctattta gttaaatata gttaacttga
    27001 tatataataa gataatacat ataattaagt aaaagattgt tttttagttt atgtttaaat
    27061 acataattac actttgttaa aaacataaaa ttattactaa agtagaaata ggaggtttta
    27121 gcatgaattt taattataat ttgccagtaa atatcttatt tgggagcggc agaattaatg
    27181 atgtgggaaa agaagtatct aaatatggca aaaaagtatt gatagtaaca ggtaaaaata
    27241 gtacaaagaa aactggatta cttgataaaa caatagattt actaaaagac tctaaaatag
    27301 aatatgaagt ttttgataga gtagaacaaa atcctcttat aactactata tattcaggag
    27361 tagaaattat aaaatcaact ggatgtgatt gtgttttagg tcttggtggt ggaagcatta
    27421 tggatgctgc caagagtata gctttttctt ataaaaaccc aggagattta aatgattata
    27481 tatttggaat tagacaaggt gaagaagctt tacctataat cttagttcca acaacatcag
    27541 gtacaggtag tgaaggaaat tgttttgcag tacttacaaa ttcagaaact aaagataaaa
    27601 aatctataaa gaagaatagt atgtatgcaa aagcatcaat aattgaccca gaacttatgg
    27661 ttacaatgcc aaaacatata attgcatcag ttggatttga tgcacttgcc cataatatgg
    27721 aagcatatct ttctaatgga agaaaccctc ttgcagatgt acaagctatt tatgggattg
    27781 aacttatatc agaaaaccta ataaaggtat ataatgatgt taatgattta gaagcatggg
    27841 aaaaaataac acttgcaagt actattggag gaatgacgat tggtacagcg ggtacagcct
    27901 tgccacatgg aatggagcat cctgtaagtg gtcttagaga tgtaatgcat ggaaaaggac
    27961 ttgcagcttt gactcctact attatagaaa gttcatggga tagtgatatt gaaagatttg
    28021 gtaatatttc aaaggtatta ggaggaactt gtgcagaaga ttgttcagat gcaattagaa
    28081 atttccttaa aaaaatagat ttagatataa agctaagtga tttaggtatt gaaaaatcag
    28141 atatagattg gttaactgat aattgtatga aaatatcagt tgcaaatatt aagagacatc
    28201 caaagtattt taataaggaa caaataaagg aaatgtacca taagtcttta taatttgact
    28261 ttacatattt agttttatat aaaaattata aattaataat aaaaaattat aagtaattaa
    28321 tagtaaaaaa attgtgtata gaagttatat atataataaa tttgtcttaa gcttttattt
    28381 tactttctaa taaataaagg agtttaaaaa agggaagatt aattaagtaa tattttataa
    28441 aattcttcga tttaaaaact ttaagtagag gtgaaataaa tgtttaatcc caaaagaata
    28501 atattcgaaa aaggaacttt agacactgat gtgggtaaaa aaatatacaa taaatttaga
    28561 gataataaag atgtagagat aattaatata tcatcaaata ggattaaaga acatattcca
    28621 ggtgaaaatc tatatgaaca atatagaagt gggaagcaga ctatggtagt tgggattaga
    28681 aaaagtctga agtttcaaac atgtaaacct tctgctaatt atcaactacc acttgtcagt
    28741 ggatgtatgg gaagatgtga gtattgttat ttgaacacac aattaggtga taaaccattt
    28801 ataagggtgt ttgtaaatgt agatgaaata cttgaacaga caaaaaaata tattgaagat
    28861 agaaagccac aggtaaccat atttgaaggg gcagctacat cagacccagt tccagtagag
    28921 ccatatacta attctttgaa gaaaacaata gagttttttg gaagagagga aaaaggaaga
    28981 tttagatttg ttactaaata cagtgatgtg gatagcttat tggatgctga acataataat
    29041 catacagaaa tacgatttag tatcaatact cctagaatta taaatgaaca tgagcattat
    29101 acagcctctg caaataaaag aatagagtca gctgtaaaac ttgctagggc tggttatcag
    29161 attggtttca taatagcacc tgtgttttta tatgaaggtt ggaaagaaga gtataaaaat
    29221 ctgattataa gtataaaaga agctttacca aaggattttg ataaaaaaat tatatttgaa
    29281 gttatatctc atagatatac aactaaagca aaaaatagaa tactagagat ttatccagat
    29341 acactattgc caatggaaaa tgaagataga aagtttaaat acggtcaatt tggatatggt
    29401 aagtacgtgt atgataaaga acaattagat gatatgaaag agttctttct taaggaaata
    29461 aatggagtat ttaaaaatag tttaataaaa tatataatat agcaaatctt acctaaagat
    29521 ttgctatatt ttatttttat atattttatg tttataataa agcaatgaga tttagcatta
    29581 tcatataaga aaattaaaaa gggcactatc tatcaagaac attttttcta gttttgatac
    29641 tataaaggat aagagtaaca aaaatatcag gaggagttat agtatgcatg catgggagtc
    29701 tattcaaatt acattggatt taatagaagc aaatttatca gaagaaatat caattaatga
    29761 attagcaaat aaagcaaatt tatcaccatt ttactatcaa agactattta aaagattggt
    29821 aaataagact gtaatggagt atataaagct tcgtcgtctt gcaagagctt ctgaatactt
    29881 agcagaacat aagaatcgta tacttgatgt agctttaaat tttggatttg ctagtcatga
    29941 gacatttaca cgttccttta aaaaagcata tggattaact ccagaaaaat atagagcaaa
    30001 tccagtaaaa ctcaatcatt ttattaagcc agaattaatt ttaaattatg ctatggttga
    30061 tgaaggtgtt ccactgattg cagatgatat agtgattgaa gtcaatcgta agacattgag
    30121 taatccaagg acatttgttg gaatagaaat agaagtacct atttgccagt tggtgggcgg
    30181 tgaaacaact ggaattgcaa tagtagagga attatggatg aaacttggaa gtcaaagaca
    30241 taatatacca aatcaaattc caggtggaaa tgaatttgct gctctttata tgggaaatgc
    30301 aaaagaagga aattgtatgt atatggcagg tgtagaggta gagtcaggta catcagtaga
    30361 aggatatagt acttttgagt tgccagctaa agaatattta gtatgtggat ttgaggcaga
    30421 aaactttaat gagttagtta attcagctgt ttttaaagca gataaattta tggaaagatg
    30481 gatgaagaaa cataatttaa caactactga ttttgcaatt gagatgtatt accccactac
    30541 acctgaagca gcatatctgg aacattggat tgttccggtt cctattgaac aatagaaagg
    30601 atgatgaata tgaattggca agatgcgttt tttcaagata ctaaacctac atttgagcaa
    30661 attaatgaat ttattaataa tccattatgg gacaagttaa ataaaacttt ggtttctact
    30721 tataatgtag aacctaagct tgaatacagt aaatgttcaa tgcaaagagg ttggaatgta
    30781 aaatataaaa aaagaggtaa atcactatgt actttatatc cacaagaagg ttattttaaa
    30841 gctttggtta ttgtaagtga aagcaataga gtagaggttg atttatttat taatacttgt
    30901 tgtgattata tcaaaaaaat atataatgag gtaaactttt ttaatggaag taaatggcta
    30961 atgattcaag tagatagttt attggtatta aacgatatgc ttgaactaat taaatttaga
    31021 gcttaacata tagaacagga tgaatatata agtcatcctg tttttatatt caatcagata
    31081 aattaatata tagtctataa tatgaataat ttagtaaatt taaatttact ttatctttct
    31141 acattctttg caactaagtc cacatgtgtt tttttcttgc atattagtag caccccaatt
    31201 agcaaattgt tgtaaaatag gcacaatact tttaccttta cttgttaatg tatattcaac
    31261 ttttggagga atttcattat attgtttacg atgaacaata ccagtagact ctaaatcttt
    31321 taatgattga gctagcatag tatttgtaat gccatccaat tttcttttta tgctactata
    31381 tctaattgga ttttcaactg ctaacataca tataataggt aatttccact taccaccgac
    31441 aatgtctaat gcataacgga ttggacaggt taattcttca acgtttttag ttatattttt
    31501 ttcagtattt atactcattt taattactcc ttagttatag tagtatcttt tttatacttt
    31561 atcagaaaaa actgcatact tgttttttct gtattttata ttaaaattat atcaaaggat
    31621 aatccttaat acaagtattt aaaaggggtg taagaattat gaaagcatta gaatttttga
    31681 acaaggcagg agtttattat ttagcaacag ttgatgagaa caatcaagca catgtgcgtc
    31741 ctttaggatt tgttatggaa tacaatggta aacttacatt ttgcacatct aatcaaaagg
    31801 atatgtttaa gcaacttatt tttaatccaa aggttgaact ttgttgtata gaccaaaacc
    31861 ttaatactct acgtatttta ggtgaagcag tatttgttac atcaaaagaa acacaagcta
    31921 aggcacttga aataatgcca agtttaggtc agatgtactc tgtaggagat ggtaagtttg
    31981 aaatattctc tattgataag gcaaaagtta gttgttgttc aatgagtggt aaaaagattg
    32041 atgtagagct ttaattataa ttgaatcgac atccatagcg aaaattaaga aaacgttatt
    32101 actgaaatat tgatagattt aatttaaatg caataaaaag atttttaact aatgaatatg
    32161 taaaaatttt tagttattag atgtgtattt caataatgat atactatt
//
LOCUS       DAMJYX010000032        31905 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMJYX010000032 DAMJYX010000000
VERSION     DAMJYX010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.032"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(155..757)
                     /locus_tag="PC479_001679"
     CDS             complement(155..757)
                     /locus_tag="PC479_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2804227.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     gene            complement(937..1755)
                     /locus_tag="PC479_001680"
     CDS             complement(937..1755)
                     /locus_tag="PC479_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2804228.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            complement(1758..2519)
                     /locus_tag="PC479_001681"
     CDS             complement(1758..2519)
                     /locus_tag="PC479_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804229.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            complement(3009..4121)
                     /locus_tag="PC479_001682"
     CDS             complement(3009..4121)
                     /locus_tag="PC479_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2804230.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            complement(4247..5251)
                     /locus_tag="PC479_001683"
     CDS             complement(4247..5251)
                     /locus_tag="PC479_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2804231.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            complement(5539..6387)
                     /locus_tag="PC479_001684"
     CDS             complement(5539..6387)
                     /locus_tag="PC479_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2804232.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(6423..6893)
                     /gene="trmL"
                     /locus_tag="PC479_001685"
     CDS             complement(6423..6893)
                     /gene="trmL"
                     /locus_tag="PC479_001685"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2804233.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(7087..8256)
                     /gene="nth"
                     /locus_tag="PC479_001686"
     CDS             complement(7087..8256)
                     /gene="nth"
                     /locus_tag="PC479_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2804234.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            complement(8454..10691)
                     /locus_tag="PC479_001687"
     CDS             complement(8454..10691)
                     /locus_tag="PC479_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2804235.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(10797..11711)
                     /gene="queG"
                     /locus_tag="PC479_001688"
     CDS             complement(10797..11711)
                     /gene="queG"
                     /locus_tag="PC479_001688"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2804236.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            12733..13137
                     /locus_tag="PC479_001689"
     CDS             12733..13137
                     /locus_tag="PC479_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2804237.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(13394..14389)
                     /locus_tag="PC479_001690"
     CDS             complement(13394..14389)
                     /locus_tag="PC479_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2804238.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(14673..15509)
                     /locus_tag="PC479_001691"
     CDS             complement(14673..15509)
                     /locus_tag="PC479_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2804239.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(15997..16560)
                     /locus_tag="PC479_001692"
     CDS             complement(15997..16560)
                     /locus_tag="PC479_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804240.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(16658..17587)
                     /gene="glsA"
                     /locus_tag="PC479_001693"
     CDS             complement(16658..17587)
                     /gene="glsA"
                     /locus_tag="PC479_001693"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2804241.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(18554..20221)
                     /locus_tag="PC479_001694"
     CDS             complement(18554..20221)
                     /locus_tag="PC479_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2804242.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(20295..21050)
                     /locus_tag="PC479_001695"
     CDS             complement(20295..21050)
                     /locus_tag="PC479_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2804243.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(21060..21596)
                     /gene="lepB"
                     /locus_tag="PC479_001696"
     CDS             complement(21060..21596)
                     /gene="lepB"
                     /locus_tag="PC479_001696"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2804244.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(21762..22298)
                     /gene="lepB"
                     /locus_tag="PC479_001697"
     CDS             complement(21762..22298)
                     /gene="lepB"
                     /locus_tag="PC479_001697"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2804245.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(22398..23708)
                     /locus_tag="PC479_001698"
     CDS             complement(22398..23708)
                     /locus_tag="PC479_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2804246.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(23787..24503)
                     /locus_tag="PC479_001699"
     CDS             complement(23787..24503)
                     /locus_tag="PC479_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2804247.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            24748..26019
                     /gene="sleC"
                     /locus_tag="PC479_001700"
     CDS             24748..26019
                     /gene="sleC"
                     /locus_tag="PC479_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2804248.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(26445..27101)
                     /locus_tag="PC479_001701"
     CDS             complement(26445..27101)
                     /locus_tag="PC479_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804249.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(28213..28623)
                     /locus_tag="PC479_001702"
     CDS             complement(28213..28623)
                     /locus_tag="PC479_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804250.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(28839..30221)
                     /locus_tag="PC479_001703"
     CDS             complement(28839..30221)
                     /locus_tag="PC479_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2804251.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(30232..30591)
                     /locus_tag="PC479_001704"
     CDS             complement(30232..30591)
                     /locus_tag="PC479_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2804252.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(30796..31575)
                     /locus_tag="PC479_001705"
     CDS             complement(30796..31575)
                     /locus_tag="PC479_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2804253.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT        10411 a         5812 c         2964 g        12718 t
ORIGIN      
        1 ctcaaatagg taaatacaat taatatctaa atgctattaa taagtaattt actcaaatag
       61 gtaaatacaa ttaatatcta aatgttatta ataagtaatt cactcaaata ggtaaatata
      121 attagtactt aaataaatgc tacttctcat atatttaaat aaacatagta attaaccctt
      181 gagctatacc tattaaaaac cctaagacaa gccccaaaaa ctctatatgt tttaattctt
      241 tcttagctat tcttattatt atctcttcca attcatataa atctagttca ttaatcttat
      301 cttcgaccat tttttgtata tctatacgtt catttgcttt atttattata tttttactca
      361 attcatctat actttgctta acttcagact caattatctc tcctagataa tcttgtatca
      421 tgttttttat accactcggg ataaatgaaa ctttctcatg tattataatt tttacccttg
      481 cttttatgta tcttacaact tcttccttat cttcgtcagt aatgatattt gcaagtattt
      541 cgtccataga aagaaattct tcttgaatta tttctcctac atttgctgct atttctgctc
      601 ttctctttgg tataagacct actatttcta tatttaaaat aggtatttta ataggctcta
      661 ttggtctaaa tatcagtctt atagcaacta cattcgtaac ataacctata aatccaccaa
      721 taacagctaa tatcagtatc ctaattaaat tatccattat aaaattactc cttatcaatt
      781 gtatatttgc cagctatatt gcattaatca agtagatatt ctttatactt aataactcta
      841 gttatactat agctttatat acaattataa gtcttttgtg tcgatttaaa aagaattttt
      901 ttaattttat atggatagct tttatatgct tagattctag ttttccataa ttatactaac
      961 tttattttca aaaattgctt ctacagtatc tataatttcc tttgagcaat tattatttaa
     1021 caaatccaat atttctattt ttttgggaca tagagttaat aaggtactta ttaaaaagtc
     1081 ctcataattt aaattttctc ttattaccat atttataatt tcttcatcat ctatactgtc
     1141 tatcttattt ccatttttat cgtataaaac aaatgtatta tcttccatta tatgaacttt
     1201 aagcaaatca atcttttctt cttgagtatc tataaagtat tttaaaactc ttataaattc
     1261 atcttggtct ttctttatta aatactcctc aactgcaata tcacctatag cagaaatata
     1321 tttcataaat tctctcattc taaatctaat aaaaccttct acatttataa aatcattttc
     1381 ttctatataa tttctcaccc tagtaaatac tgtttctctc ataaaaatct cttttttagc
     1441 aaatatgttc agtgaatata tgtatatatt atttttatct tcaggatatg tacgcccata
     1501 gcattttaga atgtattctt tcaaaagttt ctctttcatt atacttatta tcatactagt
     1561 tatttccgtt gatatctcat caatatttaa agctctttgt tcagctttag taccattaag
     1621 tattttttta tttaacactt cttgctttgc agaatctatc actacttcta caatgtcatt
     1681 cgtatatatt aatctatcgt ttacattatt attcctcaaa aatttcatgg aagaaatatt
     1741 atccttctca accaaagtta tcgctccttt cttgtcatga gaggaactat agaacctata
     1801 tctagtaaat tttcacagct aagtgtaact atctccttgt ttttactttc tacaaaactt
     1861 tttatacgtt catagtcagg atgtttttta acatctccac aaaaagctag tttatcctta
     1921 gaaatcaacc ctgtacatcc acctataaat ccataattca tctcaaataa atttatgtga
     1981 cctttttcta tcaataaaca ctctatttct gtattcttca tacttttcca aataccttta
     2041 tctgatgtta ttatagacgt tctatctact atacagattg agcatttaga atagccctgc
     2101 tctatattta tctttttaag tttattattt tcaatataat ctaatattgc tttgtcagta
     2161 tacttaaaat tgtgtatagc atagtcacca actatcgcaa tattgtaagg tatatcataa
     2221 gggtatctat ttaataaatt agcttctccc ttaattatat taaatttata tctacttaat
     2281 aactctttgt attgattata tacattcggc gcaacaatta tatctccttt tcctaaatta
     2341 caaatacata tatccgggtg atattttatg ctttcatata attctttaca ttctattgtt
     2401 tttattatct ctatatttct taattttaat tcattttcca ttagataact aattcttttg
     2461 tccaccaaag ctagacacaa atcgtcatct agtataaatg gttctcttgt aaattccatt
     2521 ttttactcct ttttcttttt tttgataaat tttttatgtg aatataaata aagtgtgtag
     2581 cagaattttt ctgcccacac acttattgtt ataattttat atagttattc taagcttaat
     2641 tagtatagct ttgtacagtt ggttcaaatt taataaatat atctttttat cactaatcta
     2701 aacttaagca gtatattccg tacagttagc acaaatttaa taaatatatc tttttattgc
     2761 taatctaaac tcaatcaata tattttgtac agttagacta aatttaataa atatatcctt
     2821 ttatcaccaa tctaaactta agcagtatat tccatactgt taacacaaat ttaataaata
     2881 tatcttttta tcactaatct aaacttaatc aatatatttt gtacagttag cacaaattta
     2941 ataaatatat ctttttattg ctaatctaaa cttaagcaat atatttttat aatcttctac
     3001 atcaataatt aatatgtatc tttcactttg ttatactctt tttttatgta ttccaaaaca
     3061 tctggtggta tatttctact gctctttgaa gtcaagccaa gttccccatc ttttatttta
     3121 aattccatta ctttacttcc cttaaaacca tcttttgcaa cgttctttat cagttcatat
     3181 acaggttgtt ctacatcttt tataattgaa cttattacat tatctggagc catatcgtat
     3241 tggtctttat taatagctat aactttacca ccattttctc ttgctacctg aatagctctt
     3301 ttactatcat cttctgtagc tgaaaataca acatctctat cagaatctct catttccttt
     3361 gcaatagttc tagctgattc agggttttca aatatatttg aatacttaac tagtaactca
     3421 gcatctttat ttgaagtttt aagccccttt ctaaatccag attcatactt atctctacta
     3481 gaaccctcaa ctcctcctat aaaaccgact ttcccagtat cagtcatttt acctgcaata
     3541 agtcctgcta aatatccagt aacattatct tgaaatgata cagaaattac attagctggc
     3601 tgaactttat aagtatagtc aactattgca aattgctgtt tgggatattt agtagcagct
     3661 tctttaattg gctctgcaaa tctggctcca actgctatta taaaatctgg ttcatactta
     3721 tgcagttttt ctatactctc tttaaaactt ttagaagtct ttggtttttc aagttttatc
     3781 ctaacatcaa aatcttcgtc agcccttttt aaggctgata gtacattgtt attatatgat
     3841 tcgttattta agttttcggt atctaacaat actccaatag tttttcttac atatgaatca
     3901 tcttctgtgt catcttcttt attcttactt ttactatttg atttatcctt ttctgctgaa
     3961 gttagcaaac ttttagactc actctgtttc gatttagcac tatctatatt agaattattc
     4021 ttatttactt tatcaaaccc aaaatcaaaa ttacatatag ctaatataaa aattgtaaaa
     4081 actactacaa agcaagccat caatatcctt tttattttca caaaattcac tccctgattt
     4141 agataggtct gacctacctc tgcatatgtc tttctatata tgtctattat aacatccaat
     4201 tcacctcata actaaactca taagtattct tggatattat agtagattat ttattgttct
     4261 tattaacata ttctaacaca tctggaggaa tattttgaga actattttgg gcaagtccag
     4321 tacttccaga cttaactgtg ttttctataa cctttccgcc tttataattt ccttttacaa
     4381 aactttgtat cagattataa gtaggattct caaaatcttt tacaatagaa cttataacat
     4441 tttctggagc taactcatgt tggtctttat tcgttgctat aactttttta ttttttgccc
     4501 ttactgcatc tattacagct tttgaatcat cttcagtagt agaaaaaata atatccactc
     4561 cattatccat cattttttta gctatagatt ctactgattt gctatcttta aaaacatctg
     4621 catactcaac tgaaatatcc tttatactac tattagaaaa tttaacaccc tctctaaaac
     4681 ctgattcaaa tttatctcta ctagaactct ttgttcctcc tataaaacca accttatcgc
     4741 tttcagtcat ttttccagca attaatccag ctaggtatcc agatttatta tcttcataag
     4801 aaattgaagt tatattagaa ggttgcttgt catactcata gtctataatt gcaaattgtt
     4861 gctttggata tttcttggct accatctcca atggttttgc aaatctagct cctacagcta
     4921 taattaaatc gtaattgtca ttacataaaa tatctatact attagaaaaa gttgaggaat
     4981 catctgattc cactttgttt gtatctatat taagtttctt ttgagcattg tttaatgcta
     5041 ataaagctga attattcata gcttcattat ttgttccttc aatatctagt atcattgata
     5101 cagatttttt tgaattactt ttatcgctta tatcactttt attatctttg ctagagctag
     5161 gattattact tgggttatta gcacacccaa ccaacattcc agctatcatt gctacagtta
     5221 acatcaatat caatattctt ttgaaacgca ttaaatttca ctcctcatac atttacgata
     5281 tttttatatc ctatttactt ttacatccta tttataattt ttttatatac aatttacaag
     5341 tacattttga aattttttta ataaatctta ctataaaaaa gtgtgcaata gattcttatc
     5401 tatttacaca cttccctaca aactaaatct aatatatcaa cacttaattt catccaaaat
     5461 aaatccaatt taacatctca acaattaata ttctatccta atttaataaa aaaataaata
     5521 atgtcatcca aaaatatatt atttattttt taagcatatg ataccagtaa caccaggacc
     5581 agcatgtgca cctatacaca caccaatttg gaaaaatcta atttctttag gtcttaattc
     5641 atcttgcata accttagata attgttcttt ttccttaaaa tcatcactat atcctatata
     5701 cacaatttgg tctgataaat catcaccaca atttgatttt atcaattcca tcatcttaga
     5761 aattacattc ttctttcctc ttacttgaga tatttgcgaa actaatccat ctcttatttc
     5821 aagaatcggt tttatattta atatacttcc tattgctgcc ttagtagatg atattctacc
     5881 tcccttttgt aaatattcca atgtatctac tgaaaatata acaaaagcct tctcttttaa
     5941 ttcatccaaa acaggcaata tttcagcaac agactttcct tcacttgcaa gttctcctgc
     6001 ttttattaca agtaaaccag ttcccataca aagcaagtta gagtcatatg tatatatttt
     6061 accatctaca tcattctttg ccattacagc actttgatac gtacctgtag ctgtggcaga
     6121 ccctgctata taaagtatct ctctatcctc atttgtatat ttctcaaaaa tttccttaaa
     6181 ttgggcataa gtagcttgag aagtttttgg atatacattt tcttctctaa gccttttata
     6241 aaattcatct ccagatatat caattctatc cttgtattct ctttcctcca gtataactgt
     6301 taatgaaact atatctatat catatttatc taacaaatct tttggcacgt ccgataaact
     6361 gtcacagatt atctttagat ttttcattaa gtatgctcct ttctcattta ggcttgttga
     6421 aactacctca tatttgggta tcctatttgt cttaaagcct cgtacactac aatagcaact
     6481 gaatttgata aatttaatga cctagctttt tcaacatcca acataggcac tctaatacaa
     6541 gtatccatat ttgctttcaa caaaggttct ggtaatccct ttgtttcttt cccaaatact
     6601 ataaaacaat tttcttcata tttcacatct gaatgtgact gtttagcctt tgttgtagaa
     6661 aaaaagtatt tactatttgg atacttttct tgtaattctt caaagctatc gtaatattgt
     6721 atatcagcta tatcccaata gtcaagccca catcttttta ggtgtttatc atccaatgaa
     6781 aaacccagtg gttttactaa atgtaaagtt gaacctgttg ctgcacaggt tcttattata
     6841 tttcctgtat tttgaggtat ttctggttct actaatacta tatttaatga cattttaatc
     6901 ctccctaagt atcgctttta tttatcaaat tttattttat aaacagaata acaataatat
     6961 tatactatta tataaaataa tgttaataat aatttttatt tcatgtatat aatagttttt
     7021 tatctaacat tttcacaagt attattatat cagaaaatcc ctcttgtaca acttactttc
     7081 taaaagttat ttcgtttcat taagttcttt ataataatta caatcttctt ttattgggca
     7141 acttgcacat tctggatttc tagctttaca cattcttctt ccatgaaaaa ttaatacatg
     7201 atgtgagtgt gaccatcttt cttttggtat agcttccata agtgcaaatt ctgttttttg
     7261 aggatttggc tcgtccacta ttcctattct attgctaact ctaaatacat gagtatctac
     7321 agctattgct ggatgattaa aagcgttact aagtacaacc cctgccgttt ttcttccaac
     7381 accaggtaat tttataagct tttctaagct atcaggtact tcaccatcat ataattcaca
     7441 tagctgttca gatgtatcct ttattttttg agacttgctt ttgtaaagac cacaactctt
     7501 tatctcttta ctaatttctt caatgctcaa attagcaaag tctctagctg tgttgtactt
     7561 tttaaacaac tctgaagtaa ctttattcac tcttacatca gtacattgtg ctgataaaat
     7621 agttgctatt aaaagttcaa aggctgtccc ataatttaac tcacatttag catctggata
     7681 caatttttct aactcgtcta aaatcttatt tacgtctttt tcatttgctg actcattttt
     7741 aattttagac ttacttttag tttctttact tgttttttgt gtagccttat tagaactact
     7801 cttctttgtt tttttctccg tttcaccttt tttagtactt tttttactta ctttgatact
     7861 ttttttatct tcttcaatat ccataaaatt ctctatatct ttctcatcaa ttatctcatc
     7921 ttttttacca atcattttat ctattccttt ttcatttatc tcttccttct caattatatc
     7981 tttcttattc attccttttt tatttatctc ttccttctca attatatctt tcttattcat
     8041 tcctttttta tttatctctt ccttctcaat tatatctttc ttatctattc cttttttatt
     8101 tatctcttcc ttctcaatta tatctttctt atctattcct tttttattta tctcttcctt
     8161 ctcaattata tttttcttat ccattccttt tttatttatc tcttcttcca aaattacatc
     8221 tttttcattt atctctttta gatttacatt tttcataatt atcccccttc aaaataccaa
     8281 tttataaaac gctatttata aatagtttaa tcatatcaac aaaaaaataa acatcaatat
     8341 ataaaacata caaaataaga atttaattta tatatattta caaaaaaagc aaaaacctat
     8401 taaagttctt gctttttatt atacataaat acccattaaa ttctataata aacttattct
     8461 tttgacacag tttgtatctt gctgaaagat attaattttt cttttaccaa tttctttatt
     8521 tcttcaaatt taacatctgt caatatctct atagcctctt ctactctttc aacagtatat
     8581 atcttaaatt ttccatctcg cactgcatca ttaacttcat ctgataaaac gagatttctt
     8641 tgattttttc taggaattat gacgccttgc tcaccattta accctttagt tttacatata
     8701 gaataaaatc cttctatctt ttcacttata ccaccaacta cttgtatatc acccttttga
     8761 tttatagaac ctgtaacagc aatattttgt ttaattgata ctccactaag agatgataat
     8821 agtacatata attctgcaga agatgcacta tctccatcta ttccaccata attttgttca
     8881 aaacaaacat atgcatttag tgacaatagt aaatcctgtg caaaattctc agaaagatag
     8941 ccacctaata taagtactcc tttgttatgt atagagccac tcatatttac ttctctttct
     9001 atatttacta tgcctttact tcctgggcta gtggtcgctg taatccttga agctctacca
     9061 aaagagtatt ctcctgtact taagactgac aaaccattta tcactcctac ttttcttccc
     9121 tctgtttcta ttagagtaaa tccattttca attgattcat ccatatgtgt ttccactcta
     9181 ttgattcttt ttctcttttc acttattgct tttttaacat cacactgctc tgtatactcc
     9241 gaatttctaa actctgcata agcacttcct tctattacaa cttccattat tttattaaac
     9301 ttagttgata atttttctaa atcaccagat aatctagtgc taaatttaat aacctcttca
     9361 actgctgatt tagtaaaatg ccttaaatta ttcttatcac attggtatgc tataaatctt
     9421 gctattcctt cttcattatc ttcattttta tccatttcac tatcaaagtc tacaaatact
     9481 ttaaaatatt tttcaaaatc ttcatcatat ctatataaaa tatcatatat atagtggctt
     9541 ccaatcagta ctaatttaac atccaatggc atattttctg gtttaattgc tgtttgtgtt
     9601 tccaatatta cttttttagt ctgcaaagtc tttttaagaa tttcccaaga cattgtatat
     9661 tttagtaatt ggtctgcata aagtactaaa taaccaccat tagccctatg aaatgctcct
     9721 gataatagtt ttgtgaaatc agtctttata tttccatttg cataatcata ctctgccttt
     9781 ccaaatagat ttgctggact tgggtttatc tctactacaa caggtgctga ttctttattc
     9841 tttgagcttc cattgtctac aaataggtta attttatatt taataaaatg ctctttatca
     9901 tacttatctt ttaactcctc ttcatctaaa tagaataaat acatatattc tattatatct
     9961 ttctttatat tttccagata agtttgaatc ttatcataat tttcatattt ttcacacaat
    10021 ctcttgatat gaggttctac tacaagttta gctatctctt cttcaagctc taacactgct
    10081 ttttcagcta tttcttctaa gtttctaatt ttatatacga cctgtatcgc catattttct
    10141 agttctcttt tagctttgta aaactcttca tcagactcat cactttcatc ttcctcttct
    10201 ttcaaaggga taaaaactat tcccatttta ctctgtttta atttaaatcc tttttcttct
    10261 ccatattttt taatttgctt caaaagcata tctttttcta tttcatattc atccagaagt
    10321 ttattttttt ctatttcaaa attttcactt tcaaacgcat ttttaaaatc ttctaaaaga
    10381 ctttcaatta atttctccat atctttctta agttctcttc caaacccctt ttctaattca
    10441 actataatag gctctctagg gttttcaaaa ttatatatat aacaccaatc tttatgcgta
    10501 ttttttttgg atgcatactc atttaaaacc tttaaggcat aagtactttt accagttcca
    10561 ggttcaccag ctatatatat gttgtacgct ggattattta ttttcaaccc taattccata
    10621 gcttctacag ctctttcttg acccaatata taatctagtg gatttatttc agatgtatta
    10681 ttatatttca atattccccc acctccccat atttaacttt aatgatataa tatgactttt
    10741 acacaacaag tgttaaagta aatacacatt aaagtatatg aaacagactc ttattattag
    10801 tcatcatact tatctaaaat tatttccata tttctcttta ttatatttct tcctctccag
    10861 ctaaaagctc tatctccata tttcctttta aattctttat ttgaaatatt ttgtatctca
    10921 tcataattca aataatctat tatattttct ttaaattcca aaatatgagt tgttggtatt
    10981 tcaacattat gtggacatac ttcttgacaa atatcgcatc caaatacctt tttgccattc
    11041 tttaaaacaa ctttttcttt ctctgacaaa tctccttttt tttgagttat atatgataaa
    11101 cattttttgg ggttcatatc ataatttccc aatatagcat ttcccggaca atattttaca
    11161 cactttccac atttaaaaca acttttttct agtggtttat cttcttcaaa tttataatta
    11221 tttactatat accctataaa tacgtaagaa ccatattcat cagttatgat attattattt
    11281 attccaaaat atcctattcc tgataaataa gctaaatacc tgtcaactaa tggtccatta
    11341 tcagcaaata acttgtattt aaaatctgtt actgttttag aaatataatc acaaatttgt
    11401 tgtaaaaagt cctttactac aatgtgataa tcctttccat gacaatattt tgataaattt
    11461 gaatttttat tcttttcttg tctatttata tgatatggaa atgcacagac tattatagag
    11521 ttagcatcct tcattatatg tcttgggtta attctgttct caattattgg ttcttccata
    11581 ccagttaaat aacctttttg ttgtctgtcc ttaagtatct tttctaaatt atcatactta
    11641 tctacccctg ctactcctac acagtttaat cctattaaat tacaaaatgc ttttaattcc
    11701 ttattatcca taaaattatt cctttttaat ctaattgact ttttaaattt atttaatttt
    11761 tttagtcttt ttaacctttt ctagctacta actcttttaa atacttaatc tttttagcta
    11821 ttcaattttc ttacctactt aaatctttta gatacttcgt ctttttacct atttaatttt
    11881 ttacctacta acgcttttag atacttaatc tttttatcta tttagttttt tacctactaa
    11941 ctcttttaga tacttcatct tttttattta ctaactcttt tagatacttc gtctttttca
    12001 tttacttatt taactttttt atctacttaa ctcctttaaa taattagtct ttttatctat
    12061 ttaattattt tacctactaa ctcttttgaa tacttagtct tttttattta tttaattttt
    12121 ttatctacta acgcttttag atacttaatc tttttatcta tttaattttt ttatctacta
    12181 actcctttat atacttagcc tttttcattt acttatttaa ctactaactc ttttagatac
    12241 ttagcctttt tcatttactt atttaacttt tttacctact taactctttt agatacttag
    12301 cctttttaat ctattcaacc ttttttaccc acttaacctt cttacctttt aatataaact
    12361 ctaaaacttc aaatatcatt agttatattt tgacattctt tctatagtta agcaaaaaga
    12421 ttgatttttt cttataatta atatcccatt tacattgcca tttattaaat ctttacttga
    12481 cttatatgga tttcttttta ctctctttac taaactatac ttattaatat tatgaaggtc
    12541 ttataataga ctaatatgaa tatgtccatc atatatcaaa ttttaaaatt tgtaagcaaa
    12601 atacttatct tatattacta catctaaagt ggaaaatttt acaaaaaagc tctgtaaaaa
    12661 cactttaaat aacaatttgc ttttaaatca tatacagatt ataataaata ctgtataagg
    12721 atggtgaaaa atttgattaa aataacaatt ccaggtagac ctattagtaa atctaatttt
    12781 aagcttcata acataaatgg tcaagcatgg atgccttcaa agggaaaata ttctaagtat
    12841 cttgcatacg aaaacatgat tgcaggtttt ataaaccaac agtatcaagg ggaaactgtc
    12901 gaagaaaatc taataacagt cttgaaatta ttcttcccaa ataagagaat gggggattta
    12961 cataattatc caaaaagtat ttgtgatggt attgaaaaaa gtggaataat aaaaaatgat
    13021 aaacaactta agccagtact tttatttgat tttatagata aagataaccc tagggttgaa
    13081 attgaacttt atccagtatc taaatacgat atatcctata atatttttga aaaataaaga
    13141 ttttatgtgt tattcgacat tatattgata ctccagtatt tgaaactata ctaatacaac
    13201 aataataatt gtgttatgtc tatactcaaa caaatgtttt taaaaagcat taagtaaata
    13261 tttaataagt attaaacaaa tattaaattt atataatagc tataactatg taaatttaat
    13321 atttgtatat aagaccacat ctaataaaaa gaaatcggag taaatttaat acaccgattt
    13381 caataaataa aatctatcct agcttttcaa caacagtctt aagcatttct cttataggaa
    13441 gttcatatgg acatctactc tcgcaaagcc cacagtcaat acactcagta ggtttaacat
    13501 ccataacctc atatttttct ttagcccact cttttaatcc atatctagta taataacctt
    13561 cacataaaaa actcaatggt atatttattc ctacagcaca aggcatacaa tattcgcatc
    13621 ttctacagaa ttttttacct aaagaatttc ttatttcttc aatttcctta ttatcttttt
    13681 catctagcac aagattttca agtacagcga cattttgtct aacctgttca acactctcca
    13741 tacctggtat aactacatct atgtactctt tagatagtat atatttaata gccagtgttg
    13801 cattgtctaa agcacctcca gctaaaggtt tcattactat tatcccaatt cctttttcat
    13861 gagctttttt aaacacttca tctgcttgtc cttcaactat attataaggg aattgtatag
    13921 tgtcaaattt tccatcttct atagcttttt ctatagtatt taaattgtga ctagttatac
    13981 caatatgttt tatcttacct tgctcctttg cctctaaaag ggcactataa gccattttat
    14041 ctttaaaaag attatcatac tcctcttctt taacattatg gaattgatat aaatctataa
    14101 aatcagtttt aaaattattt aaacttattt caacatctct cttcattgaa tcataatctc
    14161 tggacatact tttagtagct aagaaaaatt tatctctttt tccttcaatg gcaattccga
    14221 ttgcttcctc acttattgta taccctcttg cactgtcaat aaagtttatt ccttgttttt
    14281 ctaactcatt tataactaag ttagtatcat cttgagtaat cctttgtatt ggaatgcccc
    14341 caaagccaac tctctttatt ttcatgttag tattgcctaa atttctcata tctttcccca
    14401 cctaactttt aataattttt tatataatat aaatatgtca cctttaatta tacaacaaat
    14461 tatgagaatc tttgcaatgt atttacatac tattcataat ataattatat tttatatcag
    14521 tctattcata atataatcat actttatacc cagttaattt gtatacaatt ttcgttattc
    14581 ctatccatta atttaaatta tcacatttca tgattgtatc tccaccctaa ttatgttgag
    14641 ggtggagtat ttcacatatt tttaataaag gtttatttac tagatagtat ttcttttatc
    14701 attttaagct ctctcttgtg accttcatca tcaaaaggag tttctaagaa aaatggtaaa
    14761 tgttttagac taggatgttc cataaaatca attaatgctt ttaatcctat ctttccttcc
    14821 cctataggag catgtctgtc ttttttgtct ccaaatggca tcatactatc atttagatgg
    14881 acagttttca atctttctat gcctataacc ttatcaaatt cttccaaaac cccatctaaa
    14941 tcattcacta tatcataacc agctgaaaaa atatgacatg tatcaagaca cacacctatt
    15001 ttttctttat gctcaatttt gtctattata ctttttaatt gctcaaattt aaaacctatt
    15061 tcagttccct ttcccgacat agtttcaaga agtatagtta tattttcatc accctttatg
    15121 gcattgttca atccttttac tattctatct atgccaaaat ctacaccacc acctacatga
    15181 cttccaggat gaaaacacat gtattcaatt cctaatgagt ccatccttct tatatcctca
    15241 tctatcaccc ttgttgcaaa ttcgtataca tcatcctttg taccacccag attcatagta
    15301 tatggtgcat gtgcaagcag tggtgcaaaa ttattttctt ttctaatttc ttgaaattta
    15361 attatatctt tttcatcaaa ttctttagca tttcctcctc ttggatttct actaaaaaat
    15421 tgaaatgtat ttgcaccaat atatacagct gtctcagccg cattagcaaa tccttttgct
    15481 attgaaagat gtgttcctaa tttaatcata ttaatctcct tttatttgta ctactacttg
    15541 tactcaattt taattttgaa ctactccacc ttaactacat ttagccaaca tatgaagatg
    15601 gagattccta tttgatagta ctttttcact aaaaattcgt aagctctaag ggatgttcta
    15661 tcccaactaa tctcattcct cttttaacat atttctagca ttgatttaat ttatattaat
    15721 ttttaatttt aatatttaca ctttaattat acccatttta atcaatttca aatattataa
    15781 tcttacatac tttgtatatt atctaagcac ttcttagata gtgtatacat cttacactat
    15841 acatattatt tacaaataca ataggtataa aaaaacaaaa aaatagagat taaaaatcat
    15901 tgttttaccc ataccaaaaa ctattttttg attattaagt aaattttgat gttcaatctc
    15961 taatattata tctttaatta atccataatt aattctctaa ctaggacatt cctcctcaag
    16021 agtaggaact ataaccatag ctatatcata cttagaacct tcaacattgt gttgtctttt
    16081 atttaaaatt tcatctatct ttgacttcat agtgtcacta agttcaacta attcatcatg
    16141 agtaagagaa atatcatgat aatcaactat attttcattt tctaaaacat cttcctctgc
    16201 tgccttataa aaaacttcac tcatattttc aaattttgat atgtacaatt cttctttttc
    16261 ttccccattg tctaaagaaa aattaagtat cctattccct ataaccacat tttttgcact
    16321 tggataatag tacttttcca caatccccga ctttattttt tcctcaacta attctaaaat
    16381 accaactttg tataacattt ttatatggta gtttattttt gcatgaggtt cttccaataa
    16441 ttgagataat tgttttgctg acaatggcga tttgtcaaat gcttttagaa tatctattct
    16501 acgtggatgc gcaatagcct ttatgcactc caaatctcgt aataccaata cctctttcat
    16561 tgacccctcc taaaaaattt atttaattca tatttcttta caatttaatt atatattaaa
    16621 ttatatgttg ttgtcaaaaa aatcctttct taatatttta aaaaatattt aatttgagtt
    16681 tatttgacaa ctcctctaaa actttaactc ctgcgacgct gttacctttt ttgtcaagcg
    16741 caggtccata aacaccaatc cccatgcgtc ccggcacaga agccattata ccacccccaa
    16801 ctccagattt tgcaggaatg ccaactttaa tagcaaattc cccagaagca tcatacattc
    16861 cacaagtcat cataaatgtc ttgacaatcc tagaaaccat ttcactcatt aaatgctcac
    16921 cattttctat atctactcca taatttgcaa gatttattcc aattctcgct aaatctacag
    16981 aatctatctc aattgagcat tgtttaaaat aaaggtctag aacatcttct acattccctc
    17041 tgataaatcc atcacttttc aataagtaag ccatagctct gtttctatct ccagtcattt
    17101 tttcagactt atagacatca tagtttattc ctatattatc attttttgct aactttctaa
    17161 agaaactaag cattctttct tctttttctt ctaaacaact cccatttata agggaagtgg
    17221 ttactatagc ccctgcattt atcattggat tacagggttt ttttgtgtca ttaatttcaa
    17281 gtttcattat tgaattaaat gggtccccac ttggctccat tccaacgttt gaaaaaacgt
    17341 cttcccaatc attatccata agtgccatag caagcacaat tggttttgaa atactttgta
    17401 tagtaaactt tgtgctacag tttccagcac tataaagatt attttcgcta tctatgatac
    17461 aaattcctaa atcatttctt ctagcgtttt ttaattctgg tatgtaacta gcgacttgtc
    17521 cataattagt atacttctta ttagaactta taatttcttt taataatgtc tcatctaacg
    17581 tattcatatg cacctcttat attatgttat atattctatt ttagcacaag tttatactat
    17641 aataaaacaa tgttaaaaaa aattatactt taaaattatt ttctacaaga atcaattcat
    17701 ttttcaaaca atttaaatag tttaaatagc ttaataattt aataatttaa caacctagca
    17761 atttagtaat ctaataatcc agtaatttaa caacctaata atttagcaat ctaataatct
    17821 aactatctaa taatccagta acttaacaac ctagtaattt agtaattcaa caatctaaca
    17881 attcaataat ttaacaattt aacaacctag taatctaact atctaataat ccagtaactt
    17941 aacaacctag taatttagta attcaacaat ctaataattc aataatttaa caacctagta
    18001 atctaactat ctaaataatc cagtaactta acaacctagt aatttagtaa ttcaacaacc
    18061 taataattca ataatttaac aacctagtaa tctaactatc taaataatcc agtaacttaa
    18121 caacctagta atttagtaat tcaacaacct aataattcaa taatttaaca acctagtaat
    18181 ttagcaatcc agcaatctaa taatccagta atttaaattt ttataacacc aaaaatttct
    18241 aacccaacac cttttactct tttactcttt tactctttta ctcttttact cttttactct
    18301 tttactcttt tactctttta ctcttttaaa aaataatcac ataaattaac aaaatattaa
    18361 acaaaaaagt aaaatttaaa ataaaaagct tgcttcatac aaaagtataa aacaagcctt
    18421 tttatatatc aactcaaact ttaaaatctg tatcaattta attctccact ttacacttaa
    18481 tatacttaac ataaaccaat aacactactt gctatgccat ttaatcttca taaaacaatc
    18541 tataatacac acattaatca actattttac ttcctccaat aaattcccta agaattgaaa
    18601 tacctggtat gtcagaagta tcgtccaatt caacaaaata ctgcaaaaac tttagtaatc
    18661 tagtagcttc aatatgtgca ggattatctt cctttatctc ttccaacaaa ggtttcgcaa
    18721 atcgttttaa aactgccaat tcatatacac aagcagagtt aaatatagca tctgcctcct
    18781 cttggtacgg gaatatattc ttcttttctc ctcttctaac tgatgcccat tgtaaaatag
    18841 tattatcagc actatatcct ctaaaattat agtctctaac aattcttctt atcattctca
    18901 aatcagcagt agggattcga ttatggtcat ccaaatttat ttgagtaagg gcacttatgt
    18961 atatcttaaa tttatcttca tctggtatag atgatgttaa aataggattt agcccatgta
    19021 taccttctaa tataattggc tgatttgaat ctatctttat ctttttacca gtgtattctc
    19081 tcttaccttc tttaaaattg aatcttatat catcaatttc ttcaccagct aaaagtttct
    19141 ttaagtctaa gttaaacttc tctaaatcta ttgcataaat tgattcaaaa tcataatttc
    19201 catattcatc taaaggagta tgttttctat ctataaaata atcatctaaa gaaattgaaa
    19261 taggatttaa attatttact ctaagatgta ttgataatct gtgagcaaaa cttgtttttc
    19321 cagatgaaga tggagctgct attaaaacta ctctcttatt gccatccttt atcatatccg
    19381 ctatttggga taacttcttt tcatgcaatg cttcaaatgt ccttattata tctccatatt
    19441 cattgttttc tattatttta ttaagagata ctaccttgtc aattcccata agtcttgacc
    19501 attcttctgc ttcataatat acacttgata gcttaggttg aggtacaaat ttcataggaa
    19561 gagtcttatc ttcttcacta ggtcctaaaa ttataacgcc tccattataa agcttaatat
    19621 caaatgattt tatataacca gttgatggca acatataacc ataaaaatgg tctatatagt
    19681 ttctacactt atatacttta gcactatcat actctttgta tttcaaaagc tctgcctttt
    19741 catacatttc attattttca aagatagaaa tagcttcatt tcttgtaact tcaatttttt
    19801 ctataacata atcgttgttt actatctctt tcatcttgtt ttttatattt tcaacatctg
    19861 cttctttaag ctttctatct atatgaactt cacaatatag cccatctgat agtgaatgtt
    19921 ctattgtaac tctgctatcc caaaagattt ctctacatgc cattacaaat ataaatgaca
    19981 tgactctaaa gtaaactctc tctccatctt cattagtagt atctaaaaaa tttatctcac
    20041 aatccttctt aactctacaa tttagctctt ttagtttatt gtctacaact gcaagtgtta
    20101 tataaccctt atgctcatca caaaattctt ttgctatatc tcccaatctg attccagggg
    20161 aattttcttc taatgaatat tccttatctt ttccttctat ttttaagttt atttttttca
    20221 cttcaacatc tccttccttt tgatgaatat aaattttaat taatgaggca tatcaacaag
    20281 ataaagagag gtatttaacc ctctcctaca atattattca atttacttat agtatcttca
    20341 atgtcttcgt aatttatttc taatactaaa tattctgcat ctaatgttaa gatctcttta
    20401 atcccctctt cagataaaat tcctcttcct ataccaacat gtgtatcatt gataccatca
    20461 ttatcactta agtgtatcag tcttatttta tcctttaagt tctttacata agcatcctca
    20521 tcaattaaac agtgtccagt atcgtaacaa aaacaaatct tagaattttt aatcctctta
    20581 aatatatatt caaaatcata agatatattt ccaatatttg aaaaatcccc accttttgaa
    20641 taagtattct ctatacatac atatgaattt gtatttaatt tatctaaaaa atccacacta
    20701 tttcctaaat atttgtctct attctttcta actctatttg tcataacata tcccaaatga
    20761 aggttaaaat accttaaatc aaatcctttt agttcaaatt ctatcttctc aataaaattt
    20821 atccatgaat ttcgtatata ctctatgttt tcgcaagtat ttaattccat aggcaaatgt
    20881 atccctatag ataatcctaa cttagaaatt ctctctttat atttacataa ctcactacat
    20941 tcagatatat tgtcaattcc aatctctaaa tgagttattt gctttatact ctcacaaatg
    21001 tttaaagcct cctcaatatt aaacaataag gacgctatac caactgccat acaaatcacc
    21061 tactcaaaaa tacctatatc tgtaaaagga aatactctta aaataacttt tcctttaata
    21121 ttttcttcat ctactaatcc cacttcttta tatctactgt ctaaactaac ttctctattg
    21181 tctcccatcg caaatacttt tcccttagga atcaccatat caatatcgcc ttcagtataa
    21241 ttatcatgta tatatgaaac ttcatttaat aacttaccat ttatataaac cttagaatcc
    21301 ttaattttta gatggtcacc aggaacacct ataacccttt ttacaagctc ttttttcctt
    21361 ccattctctt gtaataaatc agtcttaaat atcattatat ctccacattt tggttctgat
    21421 aacttatatg tcatcctgtt tgctaccaaa taatcatgtg atttaagtgt tgggtacatt
    21481 gattctccat ttactaatgt tggttttata aaacgagtta taataaatgc aaaaaaaagt
    21541 gcagtaataa ttatttttat ccactctaca atctcttctt taatagtttc attcattcac
    21601 aaattctcct ttatagttat attatcttta ttgataatgt ctcttaaaca ctatctttta
    21661 gataagtcta ctaaataagg tgttgtcttt tttatataga caactattgt gtatggtctg
    21721 gtgatttcag ctaatcagcg attgctccaa attttatatg tttagtcaat agttcctatc
    21781 ttagaaaatg gatacaatct gactagaacc tttcctaaaa tggtatgctc gtcgaccaat
    21841 ccaacctcat cgtatctact atctaaactt ttttctctat tatctcccat tgcaaataat
    21901 tttccttctg gaactacgat atcaatatct ccatcagtac gattattatg tatataggaa
    21961 gtctcatcta acaacttatc atttacatat accttggagt cttgtatttt tacatggtca
    22021 ccaggaaccc cgataactct ttttactaaa tcttttttct ttccattttc ctctgttaag
    22081 tcggttttga atactattat atctttgtat tttggctctc ctaccttgta cgcaattctg
    22141 ttaactatca aataatcacg ttcaactaat gtaggataca ttgattctcc tttgactatt
    22201 gttggcacta taaaacgagt tattgcaaat gccaaaacaa gagctatgac aatcactttt
    22261 atccattcta caacttcttt tttaactgct tcacccacta aaatcgtctc ctctgtgtta
    22321 aatattaagt caatctacag caaaataatc taccataaac ctacaatata aatattatac
    22381 ttcttttttc taatagttta aaatactttt ctaagacctt taggataatg attcttaatt
    22441 acaccagaag attcttttcc ccaacctaaa ggtattcctt caaccgaaac caaaacccat
    22501 cctctactat cattagaatt tacaacatcc ccttttaaat atttaagaat tgattcactc
    22561 tgtacactga agttttcaac attttttaca tcttcaactt ttaagtaatg agatagtgca
    22621 tgagaaggct caaatctatt cttctttaaa acccctaaat gcaaaccata tctcaatact
    22681 tttagttttt tcgtatctgg atatatttca ggaagtaggt acaaattttc tcctcttaaa
    22741 taaaatttat tttttttatt actataaaaa tcaaccttta aaaatttctt ttcaaactcc
    22801 ctataatcct ttatttcttt atctaaattt tttattttta attctttgac attacattct
    22861 tcttcttcta atttttttat tttggcaaca aaatgacctt ctccctttat cttatgaggc
    22921 cataatcttt ccatttcaat aagttctgca tgtggatatt cactgataaa ctcatttata
    22981 acatgctcat tttcctccct tgaaaaagta caagttgagt aaattaaaat gccatccttc
    23041 tttaacattt tatatccatc tcttataatc tctttttgga tggattgaca ttctactacc
    23101 tttgcataac tccaatctga tatggcaacc tcatccttcc taaacatacc ttgcccagaa
    23161 caaggtgcat ctataataac cttatcaaaa tacccattaa acgtctttct taagctatta
    23221 gaatcggtgt ttgttataat acaatttctc gctccaaatc gctctaaatt ttcccctaga
    23281 gccttaattc tactagcatt aatttcatta gaaaccaata aacctgtatc gtttagctta
    23341 gatagtatat atgtagattt tccaccagga gcagcacaca tatcgagaac tttttcaccc
    23401 tctttaatat ctgcctttgg tattacactc attgcagaag gctcttgcaa atagtatgct
    23461 ccagcctcat gcagtggatt tctaccaggt ctgtcaattt tatcatcata ataaaaacct
    23521 tcttctgccc atggtatttg attcaaatca aacaactcca aatcaaataa ttttttcttg
    23581 tcaattttta aagtatttat tctaagccca gtagtcttac tttcctcata actttttata
    23641 aaatcatcat attcatcttt tagtatttct tcaatatctt ctaaaaattc ctttggtaat
    23701 tctgtcaaat ctataatccc ccttcaatct gctttacttg tgtgtcattt attatacttt
    23761 aattatttat gtctttcctt taaattttat ttcttcttct ttttcctaag attaccgaaa
    23821 aacacttttt tagagtgatt catatatttc tcaagtttat caagatttat cttccacaaa
    23881 attattacat aaactataag taatataata gcccatctat attctatcat caatatgaaa
    23941 tcaaaaactc catgtaataa aattggaatt aacaccgcca taaacaaata ctcacgtctt
    24001 ttatttttat ttccctcaaa tttatactta gaaatatagt agcccatagt aattgcaaac
    24061 attacatgag ctggtataga gataactgcc ctattaatac caacttgcag tgctagattt
    24121 ctactttcag aaaaaatata aaccatattt tcaattgttg caaatcctaa agctaaaaat
    24181 acagagtaaa taataccatc taacttttca gtaaagtgtt tttctttaat tagggcagga
    24241 atcagtatca gagcttttac accttcttct acaagtcctg caactacaaa tgatacatat
    24301 attagttctg aatacccttc aaatacatta aacttcatca gtaaattttc taaaagtata
    24361 attataacac ttagaaaagc acctattaaa aaaaatttaa ttagatggat taggggttct
    24421 ctacaatatc tatctttaga ccttatccaa aacatcccta tcaatatagg aattattgct
    24481 aataaaaata aatctagctt catagattat tttcctttca aaatttttga tttatttatg
    24541 atttatatca tctacttatt tttaacttat tcgtgtttta aatacatact aaaatataca
    24601 ttaaattatt ttaaattatt taaatacaaa tatactcaaa tactcaaaaa aatttaaatt
    24661 tatatctata ttatttaaca aatttcaagc tatattcata tcttgaaata taagggaact
    24721 aaattcattt aaagaaaggg tgatttgatg caagatggtt tcttaacagt aagcataatt
    24781 gatgctacta acaatagacc tattcaaaat gcagtagtaa acatatattc aatgtctaat
    24841 ggtagccaaa gctcatcaac tttataccaa aacctaagaa gtaatgaatc tggtcaagtg
    24901 actggtttag tattaccagc accagatgta gattactctc tacaaccatc tgatgttaga
    24961 ccttatagcc agtatatagt ggaagccata gcagatggat atgagacagt tgtcatagaa
    25021 ggaacacagt tgttagctac aattgaagca agacaaggag ttcccatgtc tcctagaaca
    25081 agaagtaagc gttctttctc aagacaaagt gagttgattt ttgatatagg agaacatact
    25141 ctatatggta cttatcctcc taagattcca gaaagtaact taaaaccatt accacctcct
    25201 actggtttcg ttgtattaga taaccccgta gtgcctgaat ttatagtagt tcatgatggt
    25261 ttgccagaag attcatcagc tccaaactat tggattccat ttaaagagta cattaaaaac
    25321 atagcgtcat ctgaaatata ttcaacttgg cctgaacaga caatctatgc aaatgtaata
    25381 gctataatat catttacttt aaatagagtc tttactgaat ggtatagaaa taaaggatat
    25441 aactttacaa taacttcaac aacagcatac gaccataagt ttataaataa tagaaactta
    25501 tttgaaccaa ttaacgtagt tgtagatgct atttttaata cttttataaa aagacctcct
    25561 acatcaagac agccactatt agcacaatat tgtgatggac aaaaatccca atgtcctgac
    25621 caaatgactc aatggggcag taaagactta ggtgaccaag gatatgacta tgagagtata
    25681 ctaagatatt tctatggaga tgagatagta tttgaaagag cacctatagt aagtggtgta
    25741 ccagtgtcat tccctggtac tacactacaa gttggttctt ctgggcagta tgttagaacc
    25801 atacaaaatc agttaaatgc tatatctaat tcttatccag ctgtaccaaa agttatagaa
    25861 gatggtatct atggaacaga tacagaaaac gctgttaaga tattccaagg aatctttggt
    25921 ttacctcaaa gcggagtagt agattttaaa acatggtatg aaatatccag agtttatgta
    25981 gctacaacta gaatagcttc tttaaatcct ttaatttaaa gcttgattta gaacataaac
    26041 tcaaattatt gaataaaatt attaatttaa aataaaagtg gctgttatga actaatatat
    26101 accataagta ttacttataa gtattgtagt cttaacagcc attttttagt tataacttgt
    26161 ttttttagaa tcttaaaact ttaaatgggg gattttaagc ttcttaaaaa cagtgcttag
    26221 caacttagat ttctttaaaa tattatattt atttttaagt ttaataaaat gctattctaa
    26281 cgaaaaacct actttttatt taaaatctca cttattattt aaaacactat ccatcactta
    26341 gaatattact tcttatataa attattattt attataaata caaataatat taatttaaaa
    26401 agttattcct aatttaaata atactactat cttcatcagt gaacctaaac tgcaacttta
    26461 gacttattag ctaaagccaa tattagacct aaagctatag atgcaaatgc agaagcaatt
    26521 actctatact cagcagggaa taaaaataca attgtatgac atggtatcca gaaaaatggt
    26581 atagttttta aaacactaaa accaaggtaa gcattccagt ccacaatatt taccacatct
    26641 ttaagtttta ccttaatccc ctttccaacc tcatattttt tatctataaa acagtccata
    26701 accttatgga ataaaaatac agttggtgac attagtatat tcatagttgc actcttaaat
    26761 agagcatttg caaaagctat tccctcaaat ggaagcatac catccacttg tgcttgacct
    26821 acacctgatg agtagattga cattccatat gtggtaacta cacctattat tccccaaact
    26881 attgctctat aaaatactcc tactggtatt ttccaatcct tatacaaaat tctaacacca
    26941 agtaaatctc ccattgtagc taatacagca aactttaaaa aacctccaat atatggatgt
    27001 gcacctgttg cttccataaa tacttctcta ctacttggta ttgctaagac agcaataata
    27061 caaataagaa ctccactcca tataaaatcg ccttttttca tatttataaa tactatttaa
    27121 tatataaata gttctccttt ctattaaaga tttatttaat ttacaatcta tctacaatta
    27181 tttattaatc acttattatt agatattcat tttccatatt taaatttatt ctatcccttt
    27241 atctatttat tttcttacta ctctatccac cattatctat atatttattt tacgattact
    27301 ctttcaccct aaccctttaa ctatctattt tttcttacta ttcttatcca catcatccat
    27361 atatttattt tacgattact ctttcactct aaccctttaa ctatctattt tttctgatat
    27421 tcttatccac catcatccat atatttattt tacgattact ctttcaccct aaccctttaa
    27481 ctatctattt ttttctggta ttcttatcta ctattatctg tatatttatt ttatgattac
    27541 tcttttaacc taccccctat actttatagt aatatgtttt taaccttttt cattattccc
    27601 aatatgcttt ataactacaa atactctccc cccttaaaaa taataataaa attaatcaat
    27661 aaatttataa tcatattatt actctatgta ttaagcaatt actatgccaa taaactttga
    27721 tttttttaaa caaatcttaa aaatcctaaa atgctaattt tctttaactt ttaatgaatc
    27781 taatagataa catttaattt tcgttatatt tttcagaata tttctattat tatttttaag
    27841 aaaaaaaaat aatgttaaca tttcttaaca tacattaaaa aatgttaaca ttataaattg
    27901 ggtttaatac ttttactttg actcgattta cttttattct acttcttact ttatttttaa
    27961 cttagacttt atttttttat tacttatttg agtcttattt attttattct cactccatat
    28021 gttcaattac atttgtctaa attctttgat acttttatta cataatttta acaagcttaa
    28081 gtgttatttg ggtagtgcag ttgataatta tttaaataat catcaactgc atgtttatat
    28141 cattacagta gcttaatgta tttaatcata cttacttagg attttgaaat atcttagaga
    28201 ttatatttaa cattagtcaa ttgctgcata attgtaagca attgaaattt gagacccatc
    28261 ttcattttct atagtgatag gcttcataat ttttaatcct tcagttttta tcttttctaa
    28321 ttcttgttcc attttcttaa ttgtttcctc aaggtctttt tgacttacat ctttgactgc
    28381 attagggtct ttagcatagt cttttaattc ttgaatatgt ttagcatact cttcataagt
    28441 ataatgctca acaccatact tttccaattc agtagggtca gttattcctg attttattag
    28501 agattcaaat ttaggtaaag caatattaga tgaatcattt ccatttgcaa atactgctcc
    28561 agttcctcct agtaccattg cagataccat cattgcactt ataagggatt tttttatttt
    28621 cataaccaat cttcctttct tagagattta tttgggtagt gtagtttaaa atgactattg
    28681 caatagttat caactacact cttagactat cacagtagtc atgtttatgt caagcctttt
    28741 taaaaaactt ttcaataaaa aatactatct caaagcaaaa ttaattattt tgagatagta
    28801 ttttaattct aaatctttta tattactaag atttatggtt attctatcca tgttctatat
    28861 tgttctttat taactgctaa caaattccct tttcttaata tgacaaagcg attatcatta
    28921 tagccacttc gctcaattga aacttctcct gaatcagtat aaagtacatc cttactatta
    28981 ctagatttat ttacatacgt ttcttcacta ttctcagaat tagttacttt gctcatagat
    29041 gattcttcat gctctctaaa ttcaattaca gatacttttg ccgtactaaa atgagtctct
    29101 ccatcacttt tagctttact aacaagaata tcagattcta ctttgggaga atataaatca
    29161 gttgatttac tacttattga aaatccaatt ttatcacttt gttcattttt tgttaaacca
    29221 aaagattcaa cctcattaat ctctttattt tctgttgcaa aaacaagacc tgtcccacaa
    29281 actaatacag ttgaaactaa aaaagtaaca taagttgatt ttttaaattt cataatatta
    29341 actattctct tttctatttt tgtctttcca aatccactac atattgtcat tgaaaatgga
    29401 ttattctcct ctgctagact tattagtgtg gttgcataat cagccctata ttcttcacca
    29461 agtatactca taaccttttc atcacaacta aattctatgt ctttatttag taaaaaaaac
    29521 atcacccaaa ctaagggatt aaaccaatgg acaacaagag ctacaagcat aaataacttt
    29581 aacagattat caaagctatt aatatgaacc atttcatgag ttaaaacaaa gtctaaagtt
    29641 tgattattag cataattcat ttgctttggc aaaataattc gtggttttaa aataccagat
    29701 gtaagtggtg tcctagtaaa ttcagatatg caaacaataa atcttctaaa acggctattc
    29761 ttacacatcc attttttaat gaactcatga ttttttatat gaattgaagt tttaaaaaga
    29821 ttaattattt taaaatatct aaagcataaa agtatgctaa acataatagt aactataaaa
    29881 gacaaaatgc ccaacatttc caacatcata atattttcaa tattagacat tatttcaaat
    29941 tcgtcagaat tgtttttacc tgtagagtca ctagctttct taagagtata attttctgtt
    30001 gatgtattac ccaattccac atcaaaagtc gattcaatta tactataaat actaaaacca
    30061 gaatttaaac ttactggtat tagaagttta aataaaacaa tcaaccacaa taatacaaat
    30121 acctttttag gtaatttttt aaataaacat gctcttataa aaataactat taaaagtaat
    30181 actccagaag taaaactcat ttctaacaat ttaatgaaca taaatcttac ctcactttaa
    30241 tttttcaaca atttgtttaa gttttgtaat ctcatcactt gaaagatttt ctttattaat
    30301 aaaagctgaa agaaattttt ctgaagaccc tccaaacatt ttattaatta aagtttctgt
    30361 ttcctgtttt tgtacttcct ctttgcttat taaagcttta caaacaaaat tcggctcaat
    30421 tctctctatt gcaccttttg acactaattt ttttataacg gtatacgtag tgtttctatt
    30481 ccatcctgtt tcttctttta aaatttttac taactctcca gccttgcttt ctcctctctc
    30541 ccatagaact tccattactt taagttctga atcaaataat tttatctcca tataaaatcg
    30601 ctcctcaaat atagttatca caatagtcat tactataata aaaaactatc atagtagtct
    30661 atactatgtc aatcatttat tttttaataa taaaaactgt ctttttaaaa aattcttata
    30721 aaagacagtt ttatcaatgt tatactttaa tgtttaatta aagccatatt ttatttaaat
    30781 atataactat ctatcttagt acatagtata agatttttct ttatctaata gtatatcctc
    30841 tacatctggg ttttctttta ctaattgtct ataaaaatta caattagtaa cacccacata
    30901 tggaattagc catagtagac ctattccaag agtagctatt gaaagtatat accatcctat
    30961 aaagctaaag tttagtacaa ataatttaag tacatttcct ttcatcatat ctaaactagc
    31021 acccattgat ttaaatacac ccatattatc cttgtcttca caaattaaga aagtagcttg
    31081 agaaacatat aatagcaata tataaactgg taacataaga acaagtatta taagaactag
    31141 agttattcca gtagaaataa tagtccctat tccaacatta tcacttgata aatattcaat
    31201 tgctccacta gtaagtaaaa ctccaaatga taaaaacgct cctataattg tgattataaa
    31261 agaataaata tacacaataa ttgattcaaa taaataaaaa ccaaaacttc ttaaataaat
    31321 tctcccactt acaaagatat ctcctatctt gactttttca tctttagtaa ttttcaagaa
    31381 catacatgta aatcctgctg aaacgcaaaa agctattaca gccactacaa tagtagtcaa
    31441 tggttcaaaa ggtaatccta caattgatat taatactatc aatgcaatat atactgccat
    31501 agaaattgca catatccccc agtttccaga taactgagct ttagatactc gttttaattc
    31561 ttgccttgat accataaaaa acaccccttt cattttataa aaaaactttt aaaaatataa
    31621 ataatctttt aacgtataat attaatatat catattttcg actatataag atacaattat
    31681 tcaccatgtt ttatcaagat atgccataat tgtattacta gttttatata gtcaacatta
    31741 ccaaatacat caaataaatt tgattaatta ttcaacaagt aataaaataa tttctattaa
    31801 ttatacttta tttagtaaac aaattattta ataaaaaaat tacctaatac agtaaattaa
    31861 ttggtaagat aaattattta ttattagtaa agtaaattat ttact
//
LOCUS       DAMJYX010000033        30787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJYX010000033 DAMJYX010000000
VERSION     DAMJYX010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.033"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            185..3733
                     /locus_tag="PC479_001706"
     CDS             185..3733
                     /locus_tag="PC479_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2804254.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3918..5852
                     /locus_tag="PC479_001707"
     CDS             3918..5852
                     /locus_tag="PC479_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2804255.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5958..6953
                     /locus_tag="PC479_001708"
     CDS             5958..6953
                     /locus_tag="PC479_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2804256.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6925..7710
                     /locus_tag="PC479_001709"
     CDS             6925..7710
                     /locus_tag="PC479_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2804257.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7698..8396
                     /locus_tag="PC479_001710"
     CDS             7698..8396
                     /locus_tag="PC479_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804258.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8519..8992
                     /gene="ybaK"
                     /locus_tag="PC479_001711"
     CDS             8519..8992
                     /gene="ybaK"
                     /locus_tag="PC479_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2804259.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9189..10028
                     /locus_tag="PC479_001712"
     CDS             9189..10028
                     /locus_tag="PC479_001712"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2804260.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10041..10589
                     /locus_tag="PC479_001713"
     CDS             10041..10589
                     /locus_tag="PC479_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2804261.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10655..11836
                     /locus_tag="PC479_001714"
     CDS             10655..11836
                     /locus_tag="PC479_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2804262.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12131..12517
                     /locus_tag="PC479_001715"
     CDS             12131..12517
                     /locus_tag="PC479_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2804263.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12667..12930
                     /locus_tag="PC479_001716"
     CDS             12667..12930
                     /locus_tag="PC479_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2804264.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13059..13901)
                     /locus_tag="PC479_001717"
     CDS             complement(13059..13901)
                     /locus_tag="PC479_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2804265.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14196..14930
                     /locus_tag="PC479_001718"
     CDS             14196..14930
                     /locus_tag="PC479_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2804266.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            14940..16073
                     /gene="nagA"
                     /locus_tag="PC479_001719"
     CDS             14940..16073
                     /gene="nagA"
                     /locus_tag="PC479_001719"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2804267.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16172..16921
                     /gene="nagB"
                     /locus_tag="PC479_001720"
     CDS             16172..16921
                     /gene="nagB"
                     /locus_tag="PC479_001720"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2804268.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17096..17368
                     /locus_tag="PC479_001721"
     CDS             17096..17368
                     /locus_tag="PC479_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804269.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18028..18732
                     /locus_tag="PC479_001722"
     CDS             18028..18732
                     /locus_tag="PC479_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804270.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18734..20221
                     /locus_tag="PC479_001723"
     CDS             18734..20221
                     /locus_tag="PC479_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804271.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20426..22039
                     /locus_tag="PC479_001724"
     CDS             20426..22039
                     /locus_tag="PC479_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2804272.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22402..23232)
                     /locus_tag="PC479_001725"
     CDS             complement(22402..23232)
                     /locus_tag="PC479_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2804273.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23305..25032
                     /locus_tag="PC479_001726"
     CDS             23305..25032
                     /locus_tag="PC479_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804274.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25069..26931
                     /locus_tag="PC479_001727"
     CDS             25069..26931
                     /locus_tag="PC479_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804275.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27264..27953
                     /locus_tag="PC479_001728"
     CDS             27264..27953
                     /locus_tag="PC479_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2804276.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28058..28744
                     /locus_tag="PC479_001729"
     CDS             28058..28744
                     /locus_tag="PC479_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2804277.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28761..30668
                     /locus_tag="PC479_001730"
     CDS             28761..30668
                     /locus_tag="PC479_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2804278.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT        12167 a         3220 c         5390 g        10010 t
ORIGIN      
        1 aattaagaat gatgaaatat gaataaagat tttacaaata aatcttaagc tatcttattt
       61 aattctaata caggaagaag gtaaatgtat attgaaaaaa tcatagaaat agaagtaaac
      121 aatttaaata aatacaaagg tttgcaaaag aagtaatata attatataca atgaggatgt
      181 gagtatgatg aacagactaa agccaagaat tttaggtatt agtatcttgt ttattattat
      241 aattatttta acatgtggag ctgttttcaa gtttaaattt aaattagata ataaattaaa
      301 agaaaccaca catacatatt tgtctgagtt tacagaacac aatataatta atctcaagac
      361 aagacttagt ggtcaatttg acatgcttga atctattgct aacttcatag gaaacttgaa
      421 taatataaat gatgaaatgg tgattgattt aatggattct gatgtaaagc gaagttcttt
      481 aattagaatg actatctcta cattagatgg ggaatgttat tctaatgatg gaattaccca
      541 taatgttcaa gatagagatt attttaaaaa agctaaaaat ggagaaaaga atatatcaga
      601 gcctttaaaa tctattgtta ataataaaga aataatagtt ttatctaccc cagtttataa
      661 aagtgggaaa atagttggtg ttttaagtgg aatttatgat tcaaaaaagt taaatgagtt
      721 attaggatta tctgcatttg atgaaaaagc agatgttttt gttgcaaaga aaaatggaga
      781 tatagttgct caaaaaacaa ataaaaatag aaagacgcta tcatgtatat ttcagctttt
      841 tgacaaagat aattgtgatg aagatgattg tctaaaaact ataaaagaaa atatgaaaaa
      901 tgataagtct ggatatttta attataactt agataatgaa aaatttatgt taaactatca
      961 gccacttgga gttaatgatt ggtatgtttt ttctgttgtg ccaaataaaa tagtatctat
     1021 tcagtcagag aatatatctt atgatgtata tatttttgca ttagaaatac tactagttat
     1081 attgattttt ataatatata tagcatgtat tataaataat aacaccaaaa ttattataaa
     1141 tgaaaaagaa aaatttaaag tactcactga taatatatta ggaggggtta aaacctgttt
     1201 aaaagatgaa tatatgacta tgacatatgt aagtcagggg tttttagagc ttacaggtta
     1261 ctcttgtgaa gatttaaata aaatatttaa aaatagattt aaagacatga tttataaaga
     1321 tgatttagac aagtttaatt gtattattct aaatcaatta gaaaaacaag ataaaattga
     1381 agttgaatat agaattgtaa gaaaagatgg aagtataatt tggattttgg agagaagtaa
     1441 gtctataaat gataataaag gaagtgagtg tattcaggga gttttaactg atataacaga
     1501 acttaagaaa atacaagaag aattagattc aaaaagattt gaaatacagg ctatcaatga
     1561 tagtattgtt ggtggaatag tgataactga gataaatgat gattttgata gtatatattt
     1621 aaatgaaggt tatttaaata tgattggata tacacgcaaa caactggaag atgagctaaa
     1681 taataaatta atcaaactta tttttaaaga tgatagaaca gaattaatta atagtataaa
     1741 caaacaactc aaattaagtg atacatttgc atctgaacaa agagttgaaa aaagggatgg
     1801 cactattata tggattttat taaaaggaag aaagatgatt tatgataatg gaaaatctgt
     1861 actttgttgt gttattgtag atattacttc tgctaaatta ttagagcaag aacttaaaaa
     1921 gagtgaagaa cgtcatgaaa tcattctaaa gcagacgcaa aatattataa tagaatggga
     1981 tatagaaaaa gataaaatga atatatcacc taattggtat aataagttta aatatgaccc
     2041 tataactgaa aacgcaacaa aaaatataat taaatcagta gatatatatc atgatgatat
     2101 ggataagttt atttcattaa ttaaacttac ttcacaaggt agtgagtatg aagagtgtga
     2161 aattagactt agaactagag atactattta cgcttggttt agtataaagg caacaacttt
     2221 atttgataat aatggtaaac cttataaagt agtaggaagt ttgactgata ttgatgaatc
     2281 aaaacgagaa gctgaaaagt tgaaagaaag ggcagaaaaa gaccctctta cagatctgta
     2341 taataaaaaa gcatcacaat cacttataga ggagtacata acattggata aaaaaagaaa
     2401 aggtgcttta atgattattg atattgatga ctttaaagga ataaatgata atttaggaca
     2461 tttatatggg gatgctgtat taagtgaaat tgcaaatgac ctaataaatc tatttagagc
     2521 aaccgatata gtaggcagaa ttggtggaga tgagtttatt gtatttataa aagatatttc
     2581 tgaaacaaga gatattataa ataaagcaga agaattggta aaaacatttg aaaggtcttt
     2641 tttaggtaaa ggacgaaatt ataaaatatc attaagtgta ggaattgcta agtttccaca
     2701 agatggtatg gactatatga cactatttag aaatgcggat atagctcttt actcagccaa
     2761 agggaagggc aagaattgtt atacattgta tgataaaaaa tttgataaag tccaatatat
     2821 aactaataca agaaatgcag gagatagtga taatgtagag atgtctttta aagaacatat
     2881 cacaaattat atatttgata tactatatga atcaagtgat atagaagtgg cagtaaattt
     2941 aattctaagt attgtaggta aacactttaa tgtgagtcgc atgtatatat ttgaaaattc
     3001 agatgataat cgagtatgta aaaatacatt tgaatggtgt aacgaaggtg taacctcgga
     3061 aattaataat cttaaaaata tttgttacaa ggatttaggc aactatcttt taaattttaa
     3121 tgcagatggg ttattttatt gtaatgatat tgaaaattta ggtagtgatg tgaaaaaagt
     3181 acttaagcca caaggcatac gttcaatatt acaatgtaaa attttagagg gtaatgaatt
     3241 tagagggttc ataggatttg atgaatgttc atataatcgt atgtggacta atgatgaagt
     3301 gaacactttg acatttatat ctaaaacgct cagtatgttt ttattaaaaa tgcgtattca
     3361 gagtaagcta gaagagtctt taacaattca aaaatctgtg atggataata tggatatatg
     3421 ggcatatgtt gttgacagga atacctatga attactattt atcaataaaa aaatgcttga
     3481 gtcagcacca aacactcatg taggtgattt atgttataaa gcgatatgga ataatcaaga
     3541 taaaccttgt aaaaaatgcc ctatgatata tttaaaagat ggtgatgata cttataaatc
     3601 attcatgcat aataaaaagt ttgaggtaca ggcaaatata actacaacta ctttgaaatg
     3661 gtttgatggt aaagatgcat gtttaatatt tggaagtgaa ataactagta ttgaagctga
     3721 agaaaatact taaaatactg tgttatatat atacttaaaa tcgcaatttg aatttattag
     3781 acttaaaatg tctatataaa tcatttaagt tgtggtttta agtatttttt atgatataat
     3841 taagcgttaa gaacaaaatt aaaagatact tttgtataaa aaaatatttg ttaaatatat
     3901 attgagcagg tgataatatg gaaaataagt ttttaccaat aagcaaacaa gatatgatag
     3961 atagaggatg ggaagaatta gattttgttc tagttacagg agatgcttat gtggaccatc
     4021 atagttttgg aacagcaata atatctagag ttttagaaag tgctgggtat aaagttggaa
     4081 taatagctca accagactgg agaactacag atgattttat gaagctagga aaacctagac
     4141 ttgcatttct agttaatgca ggaaatatgg attcaatggt caatcattat tctgtaagta
     4201 aaaagcatag agaaaaggat atgtattctc caggtgggaa aatgggttat agaccagata
     4261 gagctactat agtatattgc aataaaataa gagaagcata cagtgatgtt gcaatagtaa
     4321 taggtggaat tgaagcgagc ttaagaagat ttgcacacta tgattattgg agtgataagg
     4381 ttagaaagtc tatgttaata gatagtggag cagatttatt agtttatggt atgagtgaaa
     4441 agcagattgt agaagtagcc aatgcactta atgatggata tgacccaaaa tacattagac
     4501 atattgatgg gacatgctat atttcagata cattggaaga aatatatgat aaatatatat
     4561 taattccatc atataaagaa atttgtgaag ataaaatgaa gtatgtagaa gcttttaaaa
     4621 ttcaatatga tgagcaagac ccatttagag gtaatataat tgttcaacct catggaagta
     4681 agtatctggt tcaaaataag ccagaaaaac cacttagtag agaagaatta gatgaagtat
     4741 atggattacc atatcagaag acataccatc ctgtatatga aaaatttgga ggaataccag
     4801 ctatagaaga agtaaaattt agtatagtaa gttcaagagg ctgttttggt agttgttcat
     4861 tttgtgcaat aacatttcat caaggtagag cagtgcaaag tagaagtgaa aaatcaatta
     4921 ttgatgaagc aataggaata acaaatttag atgattttaa agggtatata catgatgttg
     4981 gaggtccaac agctaatttt agaagaccag catgtaaaaa acaaattact aaaggtgctt
     5041 gtaaaaatag acaatgctta tctccatcac catgtaaaaa tttagatgca gaccacagtg
     5101 aatacttaca tttacttaga gctgtaagaa aattacctaa aataaaaaag gtgtttgtgc
     5161 gttctggaat tagatacgat tatgtaatgg cagataagaa caataaattt cttagagagt
     5221 tgatagagca tcatgtaagt ggtcagttaa aggtagcccc agaacatata tcagaagaag
     5281 ttttaaagta tatgcaaaaa ccagcaggaa aaacttatga taaatttaga cagaagtttt
     5341 ttgctataaa tgaagaatta ggtaaaaagc aatatttgat accctattta atgtctagtc
     5401 accctggttc taccttaaat agcgctatag agttagcaga gtatttaaga gatactcact
     5461 accaaccaga gcaggttcag gatttttatc caactccagg gactctatct acaaccatgt
     5521 tttatacagg tattgacccg cttacaatga agccagttta tgttcctaaa tcaaaacgtg
     5581 acaaggcaat gcaaagggct ttattacaat atagagcacc tagaaattat gatttggtat
     5641 actctgcgtt agttgaagca ggaagagaag acttaattgg ttttggacat agatgtctta
     5701 taaagccaaa aaatgaaaag ccttatttta atagaaataa ttctaaaaaa aatgtatcta
     5761 aaggaacaaa taaaaacaaa aaaactaata caaataacag aagtaataaa aatcaacaaa
     5821 aaagttcaac taaaaaaaag aaaaaacatt aaagtataaa taaaaatact taaaaaagaa
     5881 ggattattcg ataaattaac agaaattagt ataaataaat ataattattt taatgacgtt
     5941 acctttggga ggaagagatg aaaaagtata tttctataat attattagtg gtgttgacta
     6001 tggttgtttt ggtaggatgt tcaccaggaa aagataaccc taaaaataag gagctatcag
     6061 ttgtcaaaca agtaaaagta gctgttccag atggactacc agcagtggca atagctaaac
     6121 ttgcaaatga aaatccagag attaaagaag gatatgaaac agtgtatagc attgaaaaga
     6181 cacctgaagc gatttccact agagtgatga aaaaagatgc agatatagct atagttcctt
     6241 caaacatggc tgcaatagcg tataataaaa cttcatcata taatattgtt ggaactgtag
     6301 gaatgggttc actttatttg gtgtcaacag aaaatataaa agattactct gatttagttg
     6361 gtaaagaagt tggatgtact ggaaaaggac ttactccaga cataactata aaaagtcttt
     6421 tgttacaaaa ggatataaat tattcagata taaaatttaa ttatgtaaat tcagcaagtg
     6481 aactggtacc attattggct actggtaaag ttaaaacagg tattgttcca gaacctgcac
     6541 ttagtgcttt gatgtctaaa aatccagaca taaaaattat aaaaagttta aatgactcat
     6601 ggaaggaagt aagtggttca aaagatggtt atcctcaatc tactttaatt gtaaagtctg
     6661 aatttttaag agataataaa gattttgttg attcttttgt aggtcaatta tcaaatagta
     6721 tagattgggc aaacaaaaat cctgaagaat taggtgcata ttctgaaaaa ataaaaatat
     6781 ctactgaatc aaagataata ggaaaatctc ttgaaagagc taatttgaaa tatattccag
     6841 ttaaggacat gattaaagat tataaaaatt actatgagaa attagctaat tttgatgata
     6901 agacattagg aggcaaagta ccagatgaag caatatactt tgttgagaaa taaaaaaaat
     6961 aaagaacaat ttatagataa aatggaggta acactttctt gtgttatcct cttattttta
     7021 tggcaaatta ttgctttaaa aattgataat gatatttttt taccaacagt aggtcaagtg
     7081 tttaatagta taaaagagat tatgctaaat tctagtttct atatagatat actttactct
     7141 atggggagat gtttttttag ttttttactg gctatgattt ttgctattat actttcaata
     7201 gtgtcttatt taaatagatt ttttagaaac ttattaaagc caatcaatgc tttgacacgt
     7261 tcaataccaa ctatgatatt ggtagtactt gcacttattt ggtttgaaaa ggatagtacc
     7321 ccttttatag ttggatttac tatagttttt cctatattgt atgataacgt tttgggcgct
     7381 atattgaata tagacaagaa cttattagaa atggctaatg tgtataaaat aaggtttata
     7441 gataaggtgt taaaaattta tttaccagcc ataaaatttc aaattatatc tatactagta
     7501 tcaacatttt ctttaagttt gaaagttgct atagcaggag aggtgtatag tcaacctaca
     7561 tatggtattg gaactatgat acagactgaa aaaattaact ttaacacatc tggaatattt
     7621 gcatggataa ttattgttct tttaatatct gctattttac aaatagctca aaagttttta
     7681 gcaaggagag cttttttatg gaaaagataa agttgagtat tgaaaatata aataaaagat
     7741 atgatagtcg tattatattt agagatttta atattgattt ttatgtaaat gaggtaaatt
     7801 gtatccttgg aaaatctggt tgtggaaaaa ctacactctt gaacataata agtgggatta
     7861 ttaaaaatga caccaacaat ctaaatatca aagaaaattt aaatagagta ggaaataagc
     7921 ttgaagctag ttacatattt caagatgata gacttataga ctggttaaca gtagaagaaa
     7981 atataaagat agttgtaaat aaatactata ataaaactca attaaataaa atttgtgatg
     8041 aatacttgga attggttgga attagtgatt ataaaaaatt ctatcctcaa atgcttagtg
     8101 gaggtattag acaaagagta aatattgcta gagcttttat atatccatca aaaaacatta
     8161 taatggatga accttttaaa tctattgatg caaagaatac acagcttata atggataatt
     8221 ttagaaatat attaagaaaa gaaaagagaa ctgtgttatt tgtaactcat aatattgagg
     8281 aagcattgtt tttagcagat aggatattta ttttaggaga ctctccaatt agaataaaaa
     8341 aaatattaaa aaattctaag gaattggaaa aaaatgaagt tttgaagttg atatagagtt
     8401 ataataatac tgtcgtttta aaatatattt ggttaaaatc tataagacta tattaaaata
     8461 gtacagaata ttcaaaactg tgtataatgt tcgtatttta taaaaaggaa gtgaatgtat
     8521 ggttaagact aatgctatga gaatattaga ttcaaataag attgattata aagtaatgtc
     8581 ttatgaagtg aaaagtgaac atgtggatgg tgttgaggtg gcacatgata taggaagaga
     8641 tgtaaatgaa gtgtataaga cattagtaac tcaaggagtt agtaagaata tatatgtata
     8701 tgtaatacca gtacatgaaa acttagactt aaaaaaggct gcaaaagtag ctaaagagaa
     8761 aagtgtagag atgattcatg taaaagatat aaataagctt acaggttata ttagaggcgg
     8821 atgttctcct ataggtatga aaaagtttta taaaactttt gtaaatgaaa gtgcaaagaa
     8881 tttagataca attattgtga gtgcaggaaa aataggatat caaatagaac tatctccatt
     8941 tgatttacaa agacttataa aagtagaatt tgtagatgta ataaaaaaat aagaattaca
     9001 tataaaatat agagcttata ttctcaataa taggtgttgt aatattaata aaatgaataa
     9061 gaattttaaa ataaatattc agttacttat gtgtaattgg tataaaaata ttgagaaatt
     9121 ttaaaaacaa tggtaaagtg taagtataat cacgaatatt ttttataaag taatggaggg
     9181 atgttcgctt gagaaaaata aaatctgaac aaatcgtcga gcaggtaaaa aaactttgta
     9241 tagaagcgag cctatattta ggagaagatg ttttaagttg tataaaagaa aaagcgaaaa
     9301 gcgaaaagag tgaagttgga aaaaatatat tgaatatatt agttgaaaat gctgaaatag
     9361 ctaaagaaaa aaatatacca atatgccaag atactggtat ggctgtattt tttgttgaaa
     9421 taggacaaga agttttaatt gaaggagaca cacttactga tgctataaat gagggagtaa
     9481 gacaagggta tgaagaaggc tacttaagaa aatctgttgt aagtccaatt aacagagtaa
     9541 atacaaaaga taatacacct gctgttattc attatgatat ggtaaaaggt gataaaatta
     9601 aaatagaatt tgcagctaag ggatttggaa gcgaaaatat gagtaagatg aaaatgttaa
     9661 agccatcaga tggattggaa ggaattaaaa aattcataat agatacagtt tcagaagctg
     9721 gacctaaccc atgccctcct atggtaattg gagttggtat aggtgggact gtggacaaat
     9781 gtgctcaaat agctaaaaag gcacttttta gagaactagg tgaatttaat aaggacgaaa
     9841 atatagcaaa acttgaaagt gaactattaa cagctattaa taaacttggt ataggacctc
     9901 aaggcttagg tggtactaca acagctttgg gattaaacat agagactttt cctacacaca
     9961 tagcaggtct acctgttgtt gtaaatataa actgccatgc atcaagacat aagaatgtag
    10021 taatttagga ggaaaataaa atgataaaaa taactacacc tgtaaatgaa attgatatag
    10081 caaaattaaa ttgtggagat acaattagtt tgagtggtat actttataca gcaagagatg
    10141 ctgctcataa aagattgata gattgtataa ataaaggtga agaattacca tttgacgtat
    10201 atggtcaagg aatatattat gtaggaccta ctcctacaaa gccaggagaa gttataggtg
    10261 ctgcaggtcc aacgacgagt tatagaatgg atgatttgac tatacctctt ttagaaagag
    10321 gtcttagatt gatgataggc aagggaaaaa gaagtgatga agttatagaa ggtatgcaaa
    10381 aatacggagc agtttatctt gctgcaatag gaggagctgg agcgtatatt tcaaattcaa
    10441 taaaatcatg tgaaattatt gcatatgaag atttaggagc tgaagctata agaaagattg
    10501 tagtagaaga ccttaagctt acagtagcaa tagatagcta tggaaataat atatatgagc
    10561 aaggtcgagc aatttatgaa tgtaaataat gtttagaaat aaatatgaat atcaaaatta
    10621 aaaatagttg tgtatatttt aaggaggaaa attgatgtct aaggattatg caaaattggc
    10681 attagaaatg catgaaatca acaaagggaa agtaagtgta gaaagcaagg tagaaattaa
    10741 aactaaggat gatttatcaa cagcatatac accaggggtt gctgaacctt gtttaaaaat
    10801 acatgaaaat caagatgatg tttatagata cacttctaaa gggaatctag tagcagtagt
    10861 tagtgatgga agtgctgtac ttggattagg taacattggt gctgaagcat ctatacctgt
    10921 tatggaaggt aaagctatat tatttaaaca atttgcagat gtagacgctt ttccaatatg
    10981 tttaaagact aatgatgtag atgagatagt taaatctgta gagttgatgg agcctgtatt
    11041 tggaggtata aatttggaag acatatcttc tccaagatgt tttgagatag aagagagatt
    11101 aaaaaaatct ttaagtatac ctgtttttca tgatgaccaa catgggacag caataatagt
    11161 tgcagccgcc ataataaact caataaagtt aattgaaaat aaaaaaatag aagatttaga
    11221 aatagttata aacggagcag gagctgctgg tatagccata gcaaagatac tacttaatat
    11281 gaatgttaaa aatataatct tatgtgatag aagtggagca ttggaagcta gtatagaaaa
    11341 tctaaactat gttcaaaaag aaatgttaaa agtaacaaat ataagaaatg aaaaaggacc
    11401 tttaaaagat attataaaag gaaaagatgt atttattggt gtttcaggtc caggagctgt
    11461 tacaaaggaa atggttgaaa gtatgtctga gaagccaata atattggcaa tggcaaatcc
    11521 tactccagag ataatgccag aagaagcaaa attaggtgga gcatttataa tgggaacagg
    11581 tcgttcagac ttccctaatc aagtcaataa tgtactggct tttcctggaa tatttagagg
    11641 tgcacttgat gttaaggcta aagagataaa tgaagaaatg aagatagctg ctgcatatgc
    11701 aattgctaat acaataagtg atgaagaaat atgcccagag tatatattac cagatgtatt
    11761 taataagaat gtagtaaaaa atgttgctaa ggcagttaaa gaagcagcga taaaaactaa
    11821 agtaaataga atataaattt ttatatgttc atatatcctt atattatcgg gtatatgggc
    11881 atttttatta taggtttata ctcatatatc cttactttta tatacatatc tttatgatta
    11941 aaggtgttag aaaatgtagt aaattttata ctatcagact ctatctttat tactttagta
    12001 gatacatggt tgtaaaggtt tatagaaaaa aatataggaa ggcagatttt tattgttgcg
    12061 tatatatgag aatgatataa tatgaatatt ataaattaat aaatttaaat ttatggaggt
    12121 acaagagatg atggaatata aagttaatga ccaagaactt aatgcttcaa tgtttatccc
    12181 attcgtcaac aggatatggc caggagatta tgatatggat aaaacacaga ctgcattatc
    12241 aaaaactttg aatataactg cttatgatga taaagtgctt gtaggatgtc tgcgagttct
    12301 ttcagatggt tattattttg gaacaattac agaattgctt gttcttccag agtatcagaa
    12361 acaaggcatt ggtagtaagc ttttccagct tgctaaagag aacaccccaa ctatgctata
    12421 ttttggtgca cagccaggag tagaagcgtt ctatgaaaaa aatgggtgcc aaagaagtct
    12481 gcaatcctac ataataaaaa agaaagatag ttattaaagt tccagctttc aataatctta
    12541 aaagtacaat attttagtgg aatcatttca ttttttatta ctaatatgac ataaacatgt
    12601 catattagta ttgatatact tttaatatgg ttaagagcca aagaaaaata taaggagtga
    12661 tttaaaatgg aagaaactaa attttgccaa tgctgtgcaa tgccaatggg acaaactgat
    12721 gaattatatg ggacaaataa agatggaagt aaaagtactg attattgtag ttattgtttt
    12781 aaagatggtg agtttacagc tgatataagt atggaagaga tgatagaagt ttgtatccct
    12841 catatgcttc aaagtaataa ggatatgact gaagatgaag caagaacaat gatgaacaat
    12901 ttctttccaa cattaaaacg ctggaaataa ttttatgcaa aattagttat atgcaaaaca
    12961 tgaaatatgg tgttgagaat aaggggtggt gtgctttata gcataccacc tgttttgtta
    13021 cttggagttc agttattttc ctaaatattt taaatatact attttagata agttccctct
    13081 tcatcttgat aaattaatcc ttttttcata agcactccaa gagctcgttt aaaatttttc
    13141 ttacttgaat taaataatat agaaatatct ttagggtcag atttatcatt aaatctcata
    13201 tatccatcag aaccttctaa ataacttaaa atcttatttt ctaaagtatc aagttcatcc
    13261 tttctatttc ctctaggaga aagaccaagt tttccatctt catatatttt tatgacatgt
    13321 aaattttcaa gaacatcacc agtttttagt tcagtaaagt actcattatg aagtattaca
    13381 ccagcatatt tattgtctac acatatcatt gcagaattat tagtttggaa tccatataca
    13441 actccagtta caatatctcc tacattgtaa gtatggtctg ttaataagta tgaatctatg
    13501 tctgtagtag cagcaattct tttgcttttg tctaaatata tataaaataa atatttttcg
    13561 tctctaaata gtggataagt cttagcttta aaaggaacta atatgtcttt aggaagtccc
    13621 atatctataa aagtaccaat gtctgtatta tcaactactt ttaaatgtgc tacatcccca
    13681 accttagcta aaggcggtat taaagttgca gcagttctgt catcagaatc tttaaatatg
    13741 aaagcattta cttcatcacc tatgtttatt tcattttttc cgattaatct attatgaaga
    13801 agtatatcgt cttttgtgtt attagtttgt ccatccaaaa aatatccaaa ttcagttttt
    13861 ctttttactg taagtttatt aaaatcacct atgtttatca aattaaaatc atcctctcat
    13921 tttatattta taatttagcc ataatatact tatgtagtag tttgtcacag tatccatatt
    13981 agtattagtg aaatttacaa taaataatat agttatttat gtatttttca attctctagc
    14041 ttcttatata gtataaactc caatttcttt tttgtcttta ggttttatta aaatttttac
    14101 aaaataagtt aaaaagtatt gaaataaaat ttcttttaaa gtataattgg tatataccaa
    14161 aattggtata taccatatcg aaaagtggtg actatatgaa aatagtaaat aaatctagta
    14221 atataccatt gcatactcaa ttaagttcaa taataagaga gatgatagaa actggtgagc
    14281 ttaaagaggg cgatgcaata atgccagaaa gagaactttg caacatacaa aatgtaagtc
    14341 gtatgacagt aaataaaacc attgttggat tagttacaga aggacttctt tatagggtac
    14401 aaggtaaagg gacattcgtt gcaaagcaaa aaaagaagta tcagttttca aatgtaaaag
    14461 gttttactga tgttatgaaa gaaaagggag taaacataaa aacggatata ttatcctttg
    14521 agatggaact accagatgat ttagttaaaa gaaagcttgg aataagtgat aatactacaa
    14581 atatatataa gattgtcaga cttagatata ctgatggaga gccatttgga ttagaaatag
    14641 tatatctatc agaagaaatg tgtaagggtc taacaaaagg gatattggat aattcttcat
    14701 tatatagggt tttaaatgaa aaatatggct ataagataca aaaagctgaa caggtaatgg
    14761 agccagtaat actttcagat gaagaaagta agcttcttga aacagatgaa ggtgctttag
    14821 ctcttaaact tcatagaaat tcctataata gagaaggtag ccctatagaa tataccatat
    14881 caatatttag aacagataaa tatcaatatg aaatagtatt gagtgagtag gtgaataata
    14941 tgaaatgttt aattaatgga aagataatat taaaaaatca aatcttagaa aataaagtat
    15001 tagtatttga tgaaaaaata attgatatag ctgatagtgt accaaaagat tgtgaagtta
    15061 tagatgctga tggaaaatat atatctccag ggcttattga tattcatata catggaaaca
    15121 tgggaaaaga tacaatggat tcgactgatg aatctattga aacaatttca aagtcaatta
    15181 tgagacatgg agtaacatct tttcttccaa ctacaatgac tatggataaa gaacatgttt
    15241 atgatgcact tgaagttatt aaaaaagccc aaaatagaaa gcttgaaggg gcacaagtat
    15301 taggagcaca tttagaagga ccttttataa atgagaatta taagggagca caaaatgaaa
    15361 aatttataat aaattcaaaa tacgaattta taaaagaata taaagatgtt ataaaagtta
    15421 taacttatgc tccagaaaaa gatattgatt ttgattttac tagagaaata aaaagatgta
    15481 cagatatagt tttatctatt ggtcatagta atgcaaatta tgaccaagct aaagaagcta
    15541 taaatttagg agtaactaat gtaactcaca tgttcaatgc aatgactggt ttaaaccata
    15601 gagacccagg cgttgttggt gcagcactta caactaatgt gtacagtgag ttaatagcag
    15661 atacaatcca tataaataaa gatttatttc aatttatact aaataataaa ggaaaagaaa
    15721 gactgatttt aataacagat tctatagaag ctggtggtct tgaagatggc aattattctc
    15781 ttggaggtca gaaagtcata gttaagggta atgaagctag acttgaaaat ggagcattag
    15841 ctggaagtgt gttgtcttta aataaaatgg tatttaactt cttagataat acaaacctta
    15901 aagtgaatga agcaattaat ttagcttctt taaatccagc aacatctttg ggaataaatg
    15961 acaaaaaagg aagcttagaa ataggtaagg atgcagatat agctgtattt gatgagaatt
    16021 tagattgtaa aatgacactt tgcttggggg acgtagtata taaaaatata tagtatacaa
    16081 aacaaacctt aaagacagta aaaacaatat aaaaaataat aaattaaact tacattagta
    16141 aaagtagtag ttaaaattta ggaggaataa gatgagagta ttagtgtgta aagattatga
    16201 tggaatgagt aaaaaagccg ctgaaatgat agcagctcaa atagttttaa aaccaaattc
    16261 aatactggga ctagctacag gaagtacacc agtaggtatg tatagagatt tagtaaaaaa
    16321 atacaatgat aatatagtag atttttcaga tgtgatgagc tttaatttag atgaatatta
    16381 taagttacca atatctaatg accaaagcta tgattatttt atgaaagaaa atctatttaa
    16441 tcatgtaaac ataaagccag aaaatactca tctaccaaat ggaatggctg atgatattga
    16501 aaaagagtgt atgaactatg aagcttctat agatgcagct ggtggtatag atgtacaagt
    16561 acttggaata ggtcgtaatg ctcatatagg ttttaatgaa cctgatacta aatttgcaaa
    16621 gagaactcat gtagttgaac ttacagagtc tacaatagaa gctaatgcaa gattctttaa
    16681 atcaagagaa gatgttccta aaaaagctgt aagtatggga ataggttcaa tattgaagtc
    16741 taagaaaata ttgcttttag cttcaggaga agaaaaagct gatgcagtat ataatactgt
    16801 ttatggagat ataactccag aagttcctgg ttctatactt caacttcata aagacactat
    16861 agttatagta gatgaagcag cagcaagtaa attgaatcca aaagattata aattagtata
    16921 agaatagaat gtagtttaag taaaaaatag ctatcattga gtaatcaaat ttatttaatg
    16981 atagctattt ttatttaaat tttttacttc tttttattta aattagttat tattgtttaa
    17041 attacttgct tattactttt tacgaaaact cgttatcaag aagtatccaa ataagatgtt
    17101 aaaaattatt atgtttagtg ggattctatc aagcataata aaattaccta taattgggat
    17161 attaccaact ggaaacataa agaagaatac aggcatcagt gatagtaaaa aagatggaaa
    17221 acaaagaaga agtaatcttg gaatatcaat ataccaacta ccatcctttt tgtattctct
    17281 aatcaataat tcagttccta gtaataaacc aataccacca gaggaaatta aattaataaa
    17341 aatgtatctt ttaaagtctt gcatataacc agtgtaaaat tgagtataga aatcataatt
    17401 taatttaaca gctaaaaaat acataagcaa tagaatgatt aaaaataaaa gatatctaag
    17461 agcttttttc attttaaatt ctcccttcag aaatacttta ttcagtttta ttttgttgga
    17521 atctgatttt atcatttagt ttaagaaatc cataatatta gtgaagaatg aaataagtat
    17581 tatatatgaa taaatatttt tagaattaag gtgaattgta gtgcttaata taactataat
    17641 attaattgtt tgaatattca ataaaaagtt tgaaaaatat caattttgat ataaactgaa
    17701 taaatacagt tgaatattaa attattattt tatcaaaaat atgcaaagta ctacaccttc
    17761 aaatatcacc taaagtaatt aaaaggcaat taaggtgaag tagttcaagt atttctaaaa
    17821 taagtttaat aaattctata ataatttatt ttttgcctta aaattgcctt aaaatccaaa
    17881 caattttaaa actaatgtac tataaaaagc tggtataatt taaatatagt aaaaaataag
    17941 atttatctta tctattggga tttaattatt taatcaatat atttatacca tttaatttaa
    18001 ataaaaagtc tattaaggag tgagcttatg agtaagttaa tttatatagc agatgatgaa
    18061 gataatataa gaaatttggt aaagacattc ttaaaaaatg aaggtcatga tgttatggat
    18121 tttaaaacgg gtgatgaact tttagaacaa tttaatataa aggagtgtga cttagtaata
    18181 ctagatataa tgatgcctgg ctcaagtggt tttgaagtat gtacaaaact tcgtgagaaa
    18241 agtacagtcc caataatcat gcttactgca agagatacag atatagatta tattacaggg
    18301 ataaccttag gtagtgatga ttattttaca aaaccattta gcccaatgtc tttggttatg
    18361 agagtaaaat ctatatttag aaggatagag tttgaaaaga aacaaaatta tgataaatat
    18421 agtaactcga tagatatgga attaaaattt ggtgatgtaa taataaataa aaagaataaa
    18481 atcgttacaa gtaaaaatgt aaatatagac cttacaccaa atgaatataa cctattgact
    18541 tatttgtttg aaaatataga tagagcagtt tccagagatg aattattaaa caaaatatgg
    18601 ggatacgata tagaagttga gacaagggct gctgatgata cagtgaagag attacgtaaa
    18661 aaaattttaa atacaaatat tttaatagaa actgtatggg ggtttggatt taggttgaag
    18721 gagaaaagtt aagatgaaga atggaaatga caagaataga attggaaaga ttaaatccaa
    18781 atttaaatta aagttctcaa agaaaaatat aagcataaaa actaggattt tgttgagtat
    18841 actttgtttg ataattatag tatttatgat aatattagta tcttttaatg tatttcttga
    18901 tacatatata aaaacaactg cgaatgaaga attgacaaag tctacagaaa tagtagaaca
    18961 tatggatagt aattttagac ctattaagcc accacaagat gatggaaagc ttcctcccaa
    19021 agtattatct aattttatga gaaatgtaca agataaagtt aggatggcag aaacacaatc
    19081 agatgctgat gctatggtag ttgattctaa gtataacctg atatttccaa caagagagga
    19141 tgatttctta aaaaatgttg atgagatgga gctgattagg gagtgtattc aaaatgaaaa
    19201 attagattta aattcagatg aaaatacacg gatatctacc agaaataagg attattatat
    19261 ttcaactgta aaaattacta gtatatatgg tgaacaagac aagtatctta tactttttat
    19321 agacatatca aaaactctaa atctagcaca aaagataaat attgtattaa tatctgtaat
    19381 gtgttttgct ggaatattag caatatttac agctgtgatt ttatctgaga aaattgctaa
    19441 accgataaaa gagttgtgtg agtttgcaaa aaaaatgggt caaggagatt tcaaaagaac
    19501 ctactttgac ttttcagata aagaattagt agagctctca actgttatga ataagagtgc
    19561 tgagtatctg gataagtatg ataatgaaca aaaaatattt tttcaaaatg catctcatga
    19621 attaaggaca ccacttatgt ctattaaagg ttatgcagaa gctataaaat ataatgttat
    19681 agatagtaaa catgcaagtg atataattct tgaagaaagt gataggttaa gtgatatggt
    19741 tgaagattta ttatacattt ctaaaataga taatataact aaagattttg aacttgtaga
    19801 atgtgattta agagaagtac tttcaaattg tggagctagg caaaatgtaa gagctataaa
    19861 taaggggata aaatttattt atgaatttga tgaagaaatg gttttatttg aatgtgatga
    19921 aaaaaacatt tccaaagcat ttatgaatct tatagaaaat gctttgagat acgctaagag
    19981 tgaaataaaa atagtgtgta agtataatca aaaaaatata gttgtgataa tagaagatga
    20041 tggaattgga atcaagaaag aagatttacc tcatgttttt gaaagattct ataagggagt
    20101 agggggaaat catggaattg gattatcaat agttaaatct attgtaaata agcatggtgg
    20161 gcgtatatac gtagaaaatg gcaaaaaagg agctaaattt actatattat ttaaattgta
    20221 aaaaaattag agaaaatata gagatgtttg aagttgtttt ttatatatat ctgttgtata
    20281 ctgttgaaaa aagaacaata ttaagctgac atatattacg atagttgaca tttaaagtaa
    20341 atgcttaaat atagatacga aaggaaaagt aaaatctgaa gtgtatttag gtttaagtaa
    20401 gcttcttatt ttataaaaaa tatgtatgaa agaacagctg atttttaata ttacaaattt
    20461 agtaacggtt gtatttgaag cttttgtcat ccatatgttt ttatcagact ttttaggtca
    20521 gaaagaaggg tatataaaag ttgtaagata tgctaaactg ggttttataa tatgtttagg
    20581 attctgtaat ttaataactt taaaccctaa aatcacaatg cctttaattt tcatccttat
    20641 tttttcaaca tcatttttat ataaaggaaa tttaaagaca cgattgttta ctactgtttt
    20701 attaagtata ttctttatac tgtcagaaat tgtagttaca agtatttttg ttttatttgt
    20761 aaaagaaggc tttgaaataa tgcttgaaaa taatagtata agagtattgg cgacaatttt
    20821 atctaaaatt gtctttttgt tgacttgtaa aattatttgt ctatttaaaa aggatgtaca
    20881 tttagatatg cccataaagt attggctccc tttattttta atacctatat ttagtttatt
    20941 tctgtctgtt tctatctttg atgtaagtaa attttttagt cttgaaagtt taaaatttct
    21001 aagcttaata tctagtgttg gtattttata tattaatttt attgtctttt atttatttaa
    21061 atttattata gataaaacta aactatctat gaaatatgaa ctacttgaaa aagaaataat
    21121 tcataaggaa gaattaagac ttagtaatga gtgttataaa attattgttg aacagactga
    21181 ttctgtcgtt ttcgaatgga atatcaaaga aaataaatca tttgtatcgc aggcttggac
    21241 tgaaaaattt ggatataaca atgcatgtaa gaatattttt aaagaaatta aagataaaga
    21301 cttagttcat tctgaagata aagctatatt tgaaggtttt cttgaaagta ttaaaaaaaa
    21361 gaatatgcac aatcaagcag tatatcgttt aaaaaaatct aatggtgaat atatttggtg
    21421 tagaacatca attacaagca tttataatga tgaaaatgaa cttttaagag ttgtcggagt
    21481 aattgtagat gtagatagtg atataaaaaa atatgaagag ttaagaacta gagcagaaag
    21541 tgactcattg accaatatat acaataaagg tacatttgaa aaattagtgg aagaaactat
    21601 agttatgaat acaggtgata aaaaagatgc attattcatt attgatttgg atgattttaa
    21661 agaaataaat gataattttg gtcatccatt tggtgatttt gtactaaaaa catttgctga
    21721 taagatacaa actagttttg gtagtaaaga cttagtcgga agaattggtg gagatgaatt
    21781 tgttgtatat atgcaagatt atgttacaga agtaaatttg cataaaaaag ctaaagaatt
    21841 aaatagagta ctttcggata attacactga tttgagtttt agttttgatg cttctgtcag
    21901 tataggtatt gcaaggtatc ctcaagatgg aacttcattt tttgaattgt ttaaaaatgc
    21961 agatagagca ctttatagca taaaagcatc aggtaaaaat tcatattgtt tatttgaaga
    22021 agaactatat gtacagtgaa tttgaattta gattttatgt gagagtaaga tttatatata
    22081 cttgtggctg tctgatttat aaaatatgtt actttgtata tttttatatg tttcagtaag
    22141 gttaattaac atgagcaaag gtgtaagtaa atttatatta gtttaagttt acttacacct
    22201 ttataaaaat acaaataata gattcattta cattcttaaa tgattaaagc tctttacctt
    22261 ctaaacatgc ataacatatc gttataattc cagtaaataa gactaaaatg cccattacta
    22321 agattacaat ttcatcatct attactccaa atgtttttaa agtaacttat ttcgctttgt
    22381 atcataataa tttgtctctt tttacatttt aacttctgct gagatttgag ttagatattc
    22441 attagaatct cgcacactta tatcatatag aagatattct tcataaacaa aatccccaat
    22501 acgtaaatta ttctgattaa aaaaatttaa taacttttca taagccttgt aaatagtttc
    22561 ataactccct ttatgataag cagttacata aagtcccttt ggtttgattg atacaggata
    22621 tttcttgtac tctttattaa cttttgtaaa taaatatgaa tatgattcaa agtctttatt
    22681 aattatatct tcacctttaa tgattgagcc aatcgtacca taatcactca attcatagtt
    22741 gtccataaag ttaatacatt catataaaaa attttcttcc tcatctccta aaaactgttc
    22801 tttatatgta acaggtgttt tgaccaagta ctcttcttca tgttctttta aagtaatagt
    22861 attcgcatct atattacatg cacttttagt aaatgaaata gtagattcca ttagagcttg
    22921 aatattattt agtttttcaa tttcattttt aatatcaatg gttttctttt ctaaaagctc
    22981 aattagtaaa ttaggtgttc ttttgtctat ataagcttta atttctttta aaggcatttt
    23041 tacttcccta agtgaagaaa ttataccaaa aatgctaagt tgatggtatg agtagtatct
    23101 atatccattt tctttcttta attctggtga gaaaagacct atatcatcat aatggaaaag
    23161 tgttttttta ttgattccac aaatctttgc aaattctcca gttgtaaaat aattttttga
    23221 aacttgactc ataattataa ttcctccttg actatcctgt tacagtatag tttatcatag
    23281 tattttgtaa aggagtgtga ttatatgaaa aaattaattc attttttaaa accatataga
    23341 gttttaattg ttgtagtttt gatatttacg tttttacaaa cacttggaac tttgtatata
    23401 ccaacactta cagcaaatat agtaaataat ggtgtagtaa agggagatat agattatatt
    23461 gtaaaaacag gtttaatgat gatgatagta gctggtataa cagctttatc agctgtgtta
    23521 gtttgtaaag tatcagcaaa tctttcatct ggattttgta gagatattag agaagcagta
    23581 tttataaaat cacaagattt atcaataaat gattttaata atataggtac agcatcaatg
    23641 attactagga gtactagtga tattacttta ataggtcagt ctgtgtttat gtttattcaa
    23701 ttagtattac cagcaccaat tataactgtt tctggtctat tcttagcata ttctatagat
    23761 aaagcaatga caattattat tgttgtagta atgtttttat tcatgctttc agcattttta
    23821 gttggtaaaa agcttataaa gttgtttaaa atgatgcaaa taaagatgga taatatgaat
    23881 agggtgttac gtgaggtagt aactggagta agagttataa gagcttttaa taggagtcgt
    23941 tttgaaaaaa agagatttga tagaactgca atagattatt ctgaaacagc aatttcaata
    24001 aataaaatat ttgcagtact tatgcctatt gtaatgttaa taatgaattt aggtatagta
    24061 agtattattt ggtttggtgg gatgagagtc agtaatggaa atatggaaat aggtcatata
    24121 atggcacttg ttgaatattg tatcttaatt ttattctatt taatcatggg agtaatggta
    24181 tttatgtaca tacctagagc aggtgcatgt gcagatagag taaatcagat attagatata
    24241 gaacctgaaa ttgtagatgg aaatggtcat aaagatactg tctcagagag aggacattta
    24301 gtatttaaaa atgtaacctt ttcatatgca caatcagaag aacctgtgtt aaataatata
    24361 acatttgaag caaaatctgg agaagtaact gcaattatag gaagcactgg ctcaggcaaa
    24421 tctactattg caaatataat accacgtttt tttgaaattc aatctggaga aatatctata
    24481 aatggtcagg atattaagaa gattcctcaa aaagaactta gagataagat tggatttgta
    24541 ccacaaaaag cttttttatt tagtggaaca atagaagaaa atataaggta tggaaaagaa
    24601 gatgcaagta ttgaagaagt aaagcatgct gcaagtatag ctcaagctga tgaatttatt
    24661 tatgatatgg aagacaagta tgactcattt gtagcacaag gtggaaataa tttgtcaggt
    24721 ggtcaaaaac agagaatatc cattgcaaga gcgttagtta gaaagcctga agtatatgtc
    24781 tttgatgata gtttttctgc attagatttt aaaacagata aaagacttag aaaagcgttg
    24841 aaaaatgaaa tcaaagattc atcagctata attatagcac aacgtataag tacaatcatg
    24901 gatgctaatc agattatagt tttaaatgat ggaaaaattg taggtattgg aaaacataaa
    24961 gatttacttg aaaattgtga agtttataag caaattgcgg attcacaact tagtaaagaa
    25021 gaacttgcat aaaatgaaga attaatctga tatttggagg tgtatgatat gagtgaaaaa
    25081 agaatagatg aacaagatat agaagtactt agttttgaag atggcatgtc agcagaaaaa
    25141 gctaaggatg caaaaaaaac tactaagaga ttattaaaat atatggcaaa acaaaagttt
    25201 aaaatattaa ttatttttat ttcagtattg ataagttcag cgcttacagt tttagcacct
    25261 atggttatgg gaaaagctat tgaccaacta tttaatggaa taaaaactgc agtccaaaca
    25321 ggtacaaaat tcagtgtgaa ttttagtact atgggaggga tagtatcaat acttctaggg
    25381 ttgtacttaa ttagtgcagt atttatatat attcaacagt atattatgtc aggagttgca
    25441 caaaatctag ttcttagcat gagaaaagat ttaagtgata aactcaataa acttcctctt
    25501 aagtactatg attctcataa aaagggggaa acacttagta tagtaacaaa tgatttagaa
    25561 aaagtagcag atagtttaca agaaggatta atgcaactta ttacagcagt agttacagta
    25621 gttggttcta ttgtaatgat gataagtata agtgtaccac ttacaatagt atcagctata
    25681 acattacttg tttcattggg tattactgtt gtaattgcaa gaaaatcaca agaaagattc
    25741 tctgaaaatc aaaaggcact aggagagtta aatagcaata tagaagagat atttactggt
    25801 caaatagtgg ttaaagcatt tagtaaagaa aaggatacta taagaaactt taaaatatta
    25861 aatcaaaatc tttataatgc aagtagaaaa gcacagtttt cgtcatatgc aatatctcca
    25921 attattagat ttataaacct aattggctat gttattatag cagttgtagg tgggatattt
    25981 gcatcaactg gagcaatgac tcttggtagt atacaagcgt ttattcaata tgtaaatcaa
    26041 gcatcagagc ctactacaga aatatcttat attgtaaata tgcttcaagc agcaatagca
    26101 tctgcagaga gagtttttac agttatggac gaggtggaag agataaagga tgaagaatct
    26161 tcaaaggtta ttagcatgcc taaaggtaaa gtacaatttg agcatgttaa atttggatat
    26221 agtgatgatt ttattcttat gaaagatatt aatatcaatc taaatgcagg agataagatt
    26281 gcaattgttg gacctacagg agctggaaag acaactcttg taaatttact tatgagattt
    26341 tatgagatac aaggtggaag aattactatt gatggtgtta atataaaaga tttaaagcgt
    26401 ggagaactta gaactatgtt tggtatggtt cttcaagata catggctgtt taatggaagt
    26461 ataaaagaaa atattgcata tagtaagtca gatgccacaa tggatgaaat tgtaagagca
    26521 gcaaaatcag caagagtaga ccattttata aggactttac cacaagggta tgacactata
    26581 ttgactgagg atgcttctaa tctatctcaa ggtcaaaagc aacttctaac aattgccagg
    26641 gcaatacttt cagacccatc agtattgatt cttgatgaag caacatctag tgtagatact
    26701 agaacagagg tagaaattca aaaggcaatg aataacttga tgaagggaag aactagtttt
    26761 gttattgcac atagattatc aactattcga gatgctgatt taatattagt aatgaaagaa
    26821 ggaactatta ttgaacaagg aagccataag gaacttattg aaaagaaagg attctatgaa
    26881 gaactttata acagtcaatt tacaagtgaa tatgatgaag atgttgtatg attatcaagt
    26941 tttaggcaac taaatgattg ataaatatta agttaaatat agtaacatgt aaggttatat
    27001 ggaataagag tatttctcta tttcatataa ccttttttat ttatcaaaat aagataaaat
    27061 ttcctttcta aattagattg aagaatgtat ttatcaatat aaaagtaaaa taatcattat
    27121 tattagtgaa gtaaatattg tttgccacat atatgccata aagatgcact tattttacgg
    27181 agatttaaca aagtagtgta aagtatactc taaatataaa ataaatcaaa tagagttaaa
    27241 taaattgaga ggtgatataa atagtgaata aaaaattgaa ctatagattt gaaatgaagc
    27301 acaaaataac tgaagcagat gtgttagctt taaaatcaag actctatcca ataatgaaga
    27361 aagatgaaaa tgcatctaaa gatggaaaat atcttattag gagtttatat tttgatactc
    27421 ctgaagataa agcacttttg gataaattaa atggcgttgc aataagagaa aagtttagga
    27481 taagatttta taataatgac tgctcttata taaggttaga gaaaaaaatc aaacattaca
    27541 acatgacatc aaagctatca actaatttaa ccaaaaaaga agtaataaat atactaaata
    27601 atgatattga atttctaaaa gagtcgacaa attgtttatt aagagaattt tatttgaaat
    27661 taaaatgtga aagattagag gcaaaaagca tagttgatta caatagagaa gcttttattt
    27721 atccagttgg aaatgtaaga gttacaatcg atagtgatat taaaacttct gtaaattcgg
    27781 tagatttatt taataaagat ttgccaacta tttcggtaat tgatgagaat atgactgtat
    27841 tggaggttaa atatgatgaa tttataccag attttataaa ggatttaatt caaattaata
    27901 agactacctc tacagcagtt tcaaagtatg cttcaagtag attatatatt taagaataaa
    27961 ggctatgatt tatataaaga tatacataaa taaggttaaa aaacaaataa gttaaaaata
    28021 aataaaataa aaaataaata agttaaaaat aggaaggatg attttaatta tgacatttaa
    28081 tgatattttt aaatcaagtt ttatagaaaa cgtatcaggt ttttcatttg tggacagtgc
    28141 tttagcacta ggttcagctt ttttagtagg tttgtttata tatatggtct ataaaaagac
    28201 ttatatggga attatgtatt ctcgaccatt caatgtgtcg cttgtagcac ttacaatgct
    28261 tacaactttt gtaatattgg cggttacttc aaatgtagtt ttatccttag gtatggttgg
    28321 tgctttatct atagtaagat ttagaacagc aattaaagac ccaatggatt tagtatttct
    28381 attttggtca ttaggttcag gtatagtttt aggagcaggt ttgattccct tagctgtcat
    28441 gggttcaata attatgggat tgattctaat attcttttca aataaaacaa tatctgaaac
    28501 tccatatata ttgatggtaa attgtaataa tgaagattca gaagatatag caactgataa
    28561 aattaaaaaa gtatttacta aatatcaaat aaaatcaaaa agtgttacac cagaaaaagg
    28621 aatagaatta gtgtttgaag taagaatgaa agatggagaa acaagtctta taaatgactt
    28681 gagtcaagta gatggagtta ctaatgcagt tcttgtaagt tataatggtg actatgtagc
    28741 atagagagga gtaatctttt atgaaagata aaaaatttac ccttcttatc tcgattatga
    28801 ttatattttt atgtgctgta gttggagttt atagtacatc tagcaacaaa agtgctgatt
    28861 tatatagtga tgtatatatt gaaaaatatt ttaacagaga caaggttatg gaagttaata
    28921 tagagataga tgaaagtgac ttgaaggata tgaatgaaaa tgctataaaa gaagaattta
    28981 aggttgcaaa agtaactgta gatggagata catatggaaa cgtaggtata agaactaaag
    29041 gaaattcaag tcttatatct gtagcaaata gtgatagtga tagatacagc tataagatta
    29101 attttgataa gtataatact agtcaaagta tggaagggct tactcaatta aatcttaata
    29161 actgttactc tgacccatct tatatgagag agtttttaac atatagtatt tgcgaggaaa
    29221 tgggattagc gactccagaa tttgcatatg ctaaagtctc tataaatggc gaatatcatg
    29281 gtttgtattt ggcagtagaa ggattaaaag agtcttatct tgaaaataat tttggtaatg
    29341 taactggaga cttatataag tcagatgaag gaagctcgtt gcaatataaa ggagatgacc
    29401 cagaaagtta ctcaaactta atcgttgaaa gtgataaaaa gacagctgat tggtctaaaa
    29461 tcacaaaact attaaaatct ttggatacag gtgaagatat tgaaaaatat cttgatgtag
    29521 attctgtcct taaaaatata gcaataaata cagctttatt aaaccttgat agctatcaag
    29581 ggagttttgc ccataactat tatttatatg agcaagatgg agtattttct atgttaccat
    29641 gggattttaa tatgtcattt ggtggattta gtggttttgg tggaggtagt caatctatag
    29701 caattgatga acctacgaca ggtaatttag aagacagacc tctcatatcc tcgttattaa
    29761 aaaatgagac atacaaaaca aaataccata aatatctgga agagatagta acaaaatacc
    29821 tagattcaga ctatttagag aatatgacaa caaaattgca tgacatgata gcatcatatg
    29881 taaaagaaga cccaacagca ttttatactt atgaagaatt tgaaaaaaat ataacatctt
    29941 caattgaaga ttctagtgat aataagggat ttggtaataa agggtttgac aacaataact
    30001 ctaataacag tgattctaat aataattcta atagtgaaaa taagcgctct ggaaatcaaa
    30061 gtgatgaaaa agaagttaat gctgaattaa catcaagcgt agtcaaagct aatacagata
    30121 atgaaactaa aaataaaact acaaatgata gtgaaagtaa gaataataca gataaagata
    30181 aaagtggaaa tgataataat caaaagctag aaggtcctat gggtaaagga ggtaagtcaa
    30241 taccaggggt tttggaagtt gcagaagata tgagtaaaac tataaaatct caattaagtg
    30301 gagaaacttc ttcgacaaag caaaactctg gtgatgaaag ttcaagtgga attaaaggta
    30361 gtgaaaagtt tgatgaggat atgagtggta tgccagaacc acctgaggga atggatggta
    30421 aaatgccacc aggaatgggt aatatggata agggagatat gaatggtaaa aatggcaata
    30481 tgaatatgga tagaaatcaa gataatccaa gagaagctgg aggttttggc aatagaggag
    30541 gaggctctgt gagtaaaaca acaacatact tcaaattaat tttaggtgga gcttcaatga
    30601 taataatgtc gattatgtta gttggtgtat caagggtaaa gagaagaaga tttataaagt
    30661 caaaataaga ttaaaattga atataaataa ttttaatact gttaagtcta aatatttact
    30721 tagcagtatt tttttagaaa ataatgtatt tggttaataa tataaggtaa taaattgata
    30781 aaaatta
//
LOCUS       DAMJYX010000034        30690 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJYX010000034 DAMJYX010000000
VERSION     DAMJYX010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.034"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            165..2066
                     /locus_tag="PC479_001731"
     CDS             165..2066
                     /locus_tag="PC479_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2804279.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2069..3112
                     /gene="ypdE"
                     /locus_tag="PC479_001732"
     CDS             2069..3112
                     /gene="ypdE"
                     /locus_tag="PC479_001732"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2804280.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3127..3432
                     /locus_tag="PC479_001733"
     CDS             3127..3432
                     /locus_tag="PC479_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2804281.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3529..3864
                     /locus_tag="PC479_001734"
     CDS             3529..3864
                     /locus_tag="PC479_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2804282.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3896..5218
                     /locus_tag="PC479_001735"
     CDS             3896..5218
                     /locus_tag="PC479_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2804283.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            5228..6298
                     /locus_tag="PC479_001736"
     CDS             5228..6298
                     /locus_tag="PC479_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2804284.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6303..7490
                     /locus_tag="PC479_001737"
     CDS             6303..7490
                     /locus_tag="PC479_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2804285.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7687..8559)
                     /locus_tag="PC479_001738"
     CDS             complement(7687..8559)
                     /locus_tag="PC479_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2804286.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8953..9144)
                     /locus_tag="PC479_001739"
     CDS             complement(8953..9144)
                     /locus_tag="PC479_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804287.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9364..10410
                     /gene="cobT"
                     /locus_tag="PC479_001740"
     CDS             9364..10410
                     /gene="cobT"
                     /locus_tag="PC479_001740"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2804288.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10442..11002
                     /gene="cobU"
                     /locus_tag="PC479_001741"
     CDS             10442..11002
                     /gene="cobU"
                     /locus_tag="PC479_001741"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2804289.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            11012..11785
                     /gene="cobS"
                     /locus_tag="PC479_001742"
     CDS             11012..11785
                     /gene="cobS"
                     /locus_tag="PC479_001742"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2804290.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11805..12419
                     /locus_tag="PC479_001743"
     CDS             11805..12419
                     /locus_tag="PC479_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2804291.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12486..12665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12795..14294
                     /locus_tag="PC479_001744"
     CDS             12795..14294
                     /locus_tag="PC479_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2804292.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14333..15709
                     /locus_tag="PC479_001745"
     CDS             14333..15709
                     /locus_tag="PC479_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2804293.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15710..16681
                     /locus_tag="PC479_001746"
     CDS             15710..16681
                     /locus_tag="PC479_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2804294.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16713..17783
                     /locus_tag="PC479_001747"
     CDS             16713..17783
                     /locus_tag="PC479_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2804295.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17785..18672
                     /locus_tag="PC479_001748"
     CDS             17785..18672
                     /locus_tag="PC479_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2804296.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18660..19292
                     /locus_tag="PC479_001749"
     CDS             18660..19292
                     /locus_tag="PC479_001749"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2804297.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19301..20527
                     /locus_tag="PC479_001750"
     CDS             19301..20527
                     /locus_tag="PC479_001750"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2804298.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20524..21132
                     /locus_tag="PC479_001751"
     CDS             20524..21132
                     /locus_tag="PC479_001751"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2804299.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21122..21697
                     /locus_tag="PC479_001752"
     CDS             21122..21697
                     /locus_tag="PC479_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2804300.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21690..22442
                     /locus_tag="PC479_001753"
     CDS             21690..22442
                     /locus_tag="PC479_001753"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2804301.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22426..23547
                     /gene="cbiG"
                     /locus_tag="PC479_001754"
     CDS             22426..23547
                     /gene="cbiG"
                     /locus_tag="PC479_001754"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2804302.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23544..24269
                     /gene="cobJ"
                     /locus_tag="PC479_001755"
     CDS             23544..24269
                     /gene="cobJ"
                     /locus_tag="PC479_001755"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2804303.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24266..25018
                     /gene="cobK"
                     /locus_tag="PC479_001756"
     CDS             24266..25018
                     /gene="cobK"
                     /locus_tag="PC479_001756"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2804304.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25037..26515
                     /locus_tag="PC479_001757"
     CDS             25037..26515
                     /locus_tag="PC479_001757"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2804305.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26545..27450
                     /gene="hemC"
                     /locus_tag="PC479_001758"
     CDS             26545..27450
                     /gene="hemC"
                     /locus_tag="PC479_001758"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2804306.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27440..28939
                     /gene="cobA"
                     /locus_tag="PC479_001759"
     CDS             27440..28939
                     /gene="cobA"
                     /locus_tag="PC479_001759"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2804307.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28956..29921
                     /gene="hemB"
                     /locus_tag="PC479_001760"
     CDS             28956..29921
                     /gene="hemB"
                     /locus_tag="PC479_001760"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2804308.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30074..30652
                     /locus_tag="PC479_001761"
     CDS             30074..30652
                     /locus_tag="PC479_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2804309.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12428 a         3063 c         5671 g         9528 t
ORIGIN      
        1 attgaactat atattttatt attataaaat atttatatta aattgtggaa ttaaaatata
       61 ctaggatata aaattcttaa ataaaaagaa aaggttgttt ttataaaaaa tattttttat
      121 accactttaa aaaacacaat aataacactg agggaggtgg ggatatgaga gaacgcatta
      181 ttcttgaaac attaaaaaat agtagtggtt atctaaacat agattatttt gcagataagt
      241 tgggggtatc tactcgaact atcagaaatg aaataaaaaa gatagaaact ataaaagaat
      301 gtaatggttt taaattagaa tataagtcta aattaggata tattctaaat attaaagacc
      361 aagataaatt cgagcattat ctaagaaact taccctcaga tttagttgaa aatccagaac
      421 aacggttgga gtctataata gtagagcttt tagtaaatga aggttataaa actatagaac
      481 agttaagtaa aaaatttcta gtaagttcat cccaaataaa aaatgattta aaaaagatag
      541 atgaaaagat aaaggataca gaattaaaac tagagcgaaa ggctcattat ggaattaaaa
      601 tagaaggttc tataaaagaa attcaaagta tattggtgga tagttatttt agagggaata
      661 gaaatataac agagtacagg aataagttta tagataattt aaaattagct aatattagaa
      721 gtacaataaa aaatgtactt aatgaacatg atttagaagc aaatttaaca gaacttgagg
      781 aaatattagc acaaattatt atattgtata taagagttag tatgagagta gtgggaaatg
      841 ctagcgattt aaaattatat acagaagatt taattataga taaattatta gataaaattt
      901 ttagagataa aaaatataac cttaattatg atgaaaagta ttatttaaag gagcttataa
      961 aactaaagac taaagataag aaagccacta taaagaatat tgataagaat aaacttcaag
     1021 atattatgtt cgaatttttt agagagatag ataaaaaata taactctaat tttttagaag
     1081 ataaagagtt ttttaactta ttttacttac atattgcatg tttaatagaa cgtataaaga
     1141 agaaccacaa gataataaat cctttttcag ttaaaataag tcaacagtat ccaactgttt
     1201 ttaatttatc tattcaattt tcaaaaatca tagaaaatga gtatcatata aaaataagtc
     1261 aagatgaaat agggtttata gctactcaca ttgctgttcc ttttgaaaag agagaagaag
     1321 ctaattttaa taaaaaatat aaaatagcaa taatatgctc ttctggtgga ggtagtgcat
     1381 ttttgataaa tttaagactt aaagaaattt ttccaaatgc tgagataaga aatttttcat
     1441 tactagaaga aaaagctgta atagaatttg caccagattt gattttttca ataacaaatc
     1501 ttttatttga aataaatgct ccagttatac ttataaatga aattttagac gaattggatt
     1561 atttagatat taaagaaagt gttagatttg cagaccatat aggaaatata tcaagcccaa
     1621 aacaatatat attaggtcta tttgataaga atcactttag gtgtataaaa gataaagcta
     1681 agtataagga tatccttgat agtatgtcaa aatgtatagt tgacgaggga gcttgttctc
     1741 caacctatcc caaagatgta tgggaaagag aaagttattt gagtactatt tatacaaatg
     1801 gggtggctat acctcatcca atcgagatga caggaaacaa aaatataata tcagtagctt
     1861 tgatacaaac tgatattatt tatgaaaata gggtgcctaa aattattttc atgatttcat
     1921 taataaaggg aaatttagaa cttcataagc aaatatctaa atatttaagt aagattatgg
     1981 caaataagga tatggtagac atgctaaata agtcacaatc ttatgaagag tttatgtata
     2041 agttaaaaat ctatctggga ggttagacat gaatatagag ttttttaaga ggttgtcaga
     2101 agcagatggt atagcatcta atgaagaaga ggttagacat gttttattgg aagaactaaa
     2161 agcttatagt gataaaatta tctgtgatgg gctagggagt atcatctttt ctaaaataaa
     2221 agatgagagt gcaccaaacg taatgatttg tgctcatatg gatgaagttg gatttatggt
     2281 gagaagtata gacaaacttg gtatgattca cttaataaca ataggaggag taaaacctct
     2341 tgcacagttt gtacaaaaag ttagaattac aacaaaagaa gggaagaaaa tccccgctgt
     2401 cataaatgct acatataata atggtaaggc tgaaaatata tatgcagata ttggagcata
     2461 tacagaagaa gatgtatata acctagggat taatgttggg gatatggtaa catatacaac
     2521 aagctttgag gaatttactt taccagaccg attagtggga aaggcttttg atgataggat
     2581 aggatgtttt gtgatgggag aagttttaaa agaacttagg aaagaaaatc taaattgtaa
     2641 tattcacttt gcggctacaa gtagtgaaga ggttggaata aggggagcaa agacatcaac
     2701 acagttaata aatccagata tagtatttgt aatagatgta gcatgtgcta aaaatgagtt
     2761 tgtgagagac tatacaaata agaaacaaat tgataaaggt gtaatgctaa tgcatagaga
     2821 taggactcta gtccctagta gaaaaatgat agattatgta atggaaattg ctgataaaaa
     2881 taatatacca cttcaacatg atatgtttga atcaggtggg actgatggag gagaagcaca
     2941 tctcgtaaat gaagggaaac cttgtgtagt aacttgtgta ccagttagat atggtcattg
     3001 tgcattttca atagtaagta ataaagattt ggagaacatt ataagattgt atacacagtt
     3061 aattttaaat tttgatgaag aacaatataa acatataaga aattttatct aaaggagaat
     3121 aagaaaatgt caacagaagt acaaataatg gaacttatat caactgcagg agagagtaaa
     3181 tcgaaagctt ttgaggcttt aaaaaaagtg aagtcaaaag attttaaagg agcaaaattg
     3241 ttaatagagg aggctcgtaa aatagatatt gaagctcaca ggatacaaac agaattaata
     3301 actcaagatt taaatgaaga tgttgaaagt ccagcaatga gtcttttaat ggtacatgct
     3361 caagaccact atatgacttc tcagctagca aaagacttga tagaggcttt gatatattgt
     3421 cttgataagt aattgtaata tagaaataga ttatttatgt aaaaattctt ttaaaagttt
     3481 agatgctttt aagagataat aaaaatacaa actttaggag gaattattat gaaaatatta
     3541 ttatgttgtg caggtggatt ttctactaat atgttaatgc aaaatatgaa aaaggtggta
     3601 aaagagagtg ctaaactcaa tgaaaaagat tttgagttta cagctatacc agcagattca
     3661 atagaaagtg aagttgatga ttgggatata gtacttatag gtcctcaaat agctcacaaa
     3721 atagacttct taaaaggaat attagagcca aaaaataagc cttttgcagt aatagataaa
     3781 gatgtatatg gaaaaatgga tggagctaca gttatgaagt tggcattaat aacttataag
     3841 aaacatcaat tagctcaagg ataataaaaa taatgaggga aaagggagat tagatatgtt
     3901 ttcaaaattt gaagcattta tgaatagagt atttatgcct ttagctcata aggttgacaa
     3961 tcaacgtcac cttggttcta taaaggctgg tatggttgct atgacaccat tcacaatatt
     4021 aggaagtata tttgcaatac ttccagcact tccaaatatg cttggagaaa ataatccaat
     4081 aagcaaattc atacttagca attcagcaat attggattta ccagtaagtc tttccatagg
     4141 tttaatagga ttatatgctt gtatgtgtat tgcttttaat ctggggaact actataaatt
     4201 atatatacca ggttgtgttg cactatcaac tttttctttt ttattcctag tagcctcatt
     4261 tacaccagat ggagctttag aaataggaaa tttaggagct aaagggctat ttacaggtat
     4321 gatagcaggg cttggttctg tggagttata taatttttgt aataagaaga atatcacaat
     4381 aaaaatgcca gaaaatgtac ctgattttgt atctaggtct tttgaattaa tacctactac
     4441 tataatagtt tgtggaactt ttatagttgc tcgttttatt tcattaaaag tatttggaga
     4501 attacctcca cagatactta ctcaattttt atctccatta gtaggaagta tggataaccc
     4561 atggtcagtt cttgcactta actttgctat atgtactata ttcttttttg gaatacattc
     4621 atcagtattt agtcctataa ctagacctat aatgattaca tttatagctg aaaacatagc
     4681 agctatgcag gctggagaaa caatacctca tttctttaca gcaggagctt caagtgcatt
     4741 ctttggattt acaggatgtg gaattagtat aggttgtgta attgcatgta tgttatcaaa
     4801 aaatgcttcc tataagaaga ttggacgtgt atcattattc cctgcattat ttgggataaa
     4861 tgaaccaata ttatttggag caccgataat attaaatcca ataatgttta taccacatgt
     4921 atttggtgga gctataatag gtacactacc aatgtttttt atgcactggg gatggttagc
     4981 aaagccagta tttgaccctc catatgtagg ggtattctta gaaggatttt taactaatgg
     5041 agattggcat tctatattag caaatgcatt gcaattagta ttagcagttt tattatattg
     5101 gccattcttt aaaataatgg aacgtcaaca aaataataaa aaacaaaaag aagaaactaa
     5161 gaatatattc tctaaagaag aagaaggctt attggatgat ttaggattgg atttctaata
     5221 ttttgttatg aataatacaa tggagagatt agtatcctta atctataaaa gaggtgttga
     5281 cgctcttttg attaaagcaa aaaatactaa aagatatata ggagctttat caggtagtgg
     5341 tgtatatgtg cttgttacta aggataaaaa ttatcaaatt ctagatggca gatatattga
     5401 tgaagcagat aaaaaaacga ctggtttcat aaaaaggatt gtatctcaag gttcttatat
     5461 accaactata attgagttat taaaagaatt aaatataaaa aagttagcaa tagaaaacca
     5521 agctatgagt atacaagagt atctttcact aaaaaatgaa ggttttgaaa tcaatttaat
     5581 aacaaatgaa ttatggaagc taagggctat aaaatctaaa gaagaaatta aacttattaa
     5641 aaaggcttgt gagattacag atgaagtatt tgatgaggtt atttctgaaa ttaaaaaaga
     5701 tatgactgag ctagaagtat cagctctaat tcagtatcat gcattaaaaa aaggtgcaag
     5761 tggaatgtct tttgaaacaa tagtggtatc aggagaaaga ggtgcaatgc cacatggaag
     5821 acctacaaac aaaaaattga aagtgaatga agctataaca atagattttg gtgttgttta
     5881 tcaagggtat caatcagaca tgactcgtac aatatctata ggcaaacctc ctaaaattat
     5941 taaagagata tatgatgtag tattggaagc acaattatct gcaatagagt caataaagga
     6001 aggaactagg gcaagtgatg ttgacaaagt ggctagaaaa attatagata aacatggatt
     6061 tggcgaatat tttaatcatg gactgggtca tggtataggt cttggagatg gtgaagttcc
     6121 cactttaaat ccaaatagtg aagatatatt agtggaagga atggtaatgt cttgtgagcc
     6181 aggtatttat attccaaatg tagggggagt tagaatagaa gatgatattg ttataataga
     6241 tggaaaaggt gttccactca ataagacatc caaagagttt ataatattag gagagtaaaa
     6301 ttatgaaata taattttaat aaagtattaa atcgtaaaga aggaaattgt agaaaatgga
     6361 gcaaccatgt gattaaagaa aaatttggct taaatgaaga tgcaattcct atggatttag
     6421 ctgatatcga ttttgagtgt gctcctgcta taaaagaatc tatcttaaaa agagcatcag
     6481 taggagatta tagttatact tttataggag atgacttcta tgatgctatt ataaattgga
     6541 ataaaagaag atttgatgta tatatagaaa aggattggat aaaattaact tttggtactg
     6601 tttcaaccct tcattatata gtccaagcat atactaaaga aggagagggt gtattgataa
     6661 atacacctgc ttatgaccct tttgcagagg ctgtagtaaa taataataga aagttgtgtt
     6721 gtagctcatt aaaattagat aacaatagat attatttaga tttagaggat atggaaaatc
     6781 aaatgaaata cgagaatata agattgctta tattctgtag tccacaaaat ccatcaggtc
     6841 gtatatggac aaaagaagaa ctatatcaag tgtcagagtt atgtttaaag tataatgtaa
     6901 ttttggtaag tgatgaaatt catagagata taatatttaa agattataga tttgtatctt
     6961 tgtggaatgc tcatccagaa atttataaaa atagtataat ctgtgtatct cctaataaag
     7021 gcttcaactt aggtggatta aaaacatcat atgtattaat aagagatgta aaaattaggg
     7081 agactttatt agaaaggcta aaaagtaatt caataacttc tccaaatgta tttgcaatac
     7141 cagcaatagt tgcagcatat aatgaaagcg aagaatggct agatgctatg actagttatg
     7201 tagaggagaa ttttgaaata gtttatgatt tctttgaaac taatatacca aaagctaaag
     7261 tgatgaagtc agactcttct tttttagcct ggatagatgt aagagaagta tttagaaatg
     7321 aagaagaatc taaagaattt tttagacatg caaacttaac tatggtagtt ggaagttatt
     7381 ttgtgaaaga tggagatgga tttattcgta ttaatgtggg gtgtcctaga gaaacattaa
     7441 atgaggctct aaatagaatc aaaaaaacgt atataagtat gtattgttaa atcataaaaa
     7501 aagtatctat ggtttatata aaaacacatt atttagttat caaatattac tggaattttg
     7561 tattccagta atattttttg attaaggaat attattgagg aatattaaat aatattattt
     7621 cataccctta tattaactta tataaaaata ttttattatt ttgggaacgg gttagctaac
     7681 ctttatctag tgtatattta tattgtaaga agcagtaaaa acttttcctt tactaagttt
     7741 attaataaac ttcttatctt tataaatttt gttagaattt atactatcag caagaccata
     7801 ccaaggttct atacataaaa aaggagcagt agaattagtt tcactataat aaggagtcca
     7861 aataccaact agagggaaat cttccataga tatagttaca tatttactac catcattatt
     7921 acatatagac acttcatcta tgttggtgta tatcaaagca tcattcttga atacttctgg
     7981 atttaactgt aagtttttta attttccaat aggtattata tcatctgtaa aagaaccatt
     8041 taaattgatt ttttctacat catttttact tttaaactca aggtagtgtt tagaaaaatc
     8101 attttgttcg taaaaaggaa ggttgaatgc aggatgtcca cctatagaaa agaatatatc
     8161 tttagaatca gtatttttta caatccattc tacctcaata ctattatcat ttaatgtata
     8221 gtttatgatt agctcaaaag aatatggata cttctttaaa gtagattcat tatctgttaa
     8281 tttataagtt acaaatgtat ctcctttatc tacaatctca aagtccatat ctctagcaaa
     8341 cccatgttga gtcatgctat ataaattttc ttctattatt gtttcattat caaaaagttt
     8401 acctactata gggaacaata taggtgattg tctaccccaa tattttttat taccatccca
     8461 aagaatgttt ttgtcgtatt ttttagaaaa tattttagtt agttcagcac caaaactgct
     8521 tgattcgata attaaatttt cattttgtaa tatattcatt taaaatctcc tttattatat
     8581 tttatgagta tattatacta taaagtataa agattattaa attttatttg atattatatt
     8641 tgaaaggtag attttagtat attttgaaaa ttttgttttg aaaatttggg attatttaag
     8701 aaattttgta ttataaatag aaggaaatag ataaattgta tagaataaaa tatattaaga
     8761 taagttaata atgttaaatg ggaagtgagt ttatggaaaa accaaataca aaagttgtta
     8821 actttcaaga gtataaagac agaaaaaacc aaagagaagt aaatagagac gaacttttaa
     8881 agcttataaa aaaaattgtg acttcaaaat agggagttgt taaactccct attttaatgg
     8941 gtttcataat aattaagaca ttctaagtcc atctatataa tctttgaatt gtgagaaatc
     9001 tgattcacta cttaattcta tggtttctga accttccgta taaaaattct taagatagat
     9061 agtataatta ttatcaacac aatcatgata aatacatcta tatccatctt catacaaact
     9121 ttttaactta gaaaaatcat ccacaatatc atctccttaa agttctttta aatatatttt
     9181 tacaaaaagc atctaattgt atgcaaattg acaatgtaaa ataaaggaaa tgaacataaa
     9241 atgtagaata atttttatat gtatgtaaaa ataaaaagaa aaagttgaaa tttttagaaa
     9301 ataagagtat tttttatcta aaaagagagt ttaaaaaata aaaaaatgtt gggaggataa
     9361 aaaatgagtt tattagaaag tatatcaaaa aacatatatt ccctagataa ctcttctatt
     9421 gaaaaaacaa agcagagatt ggataggctt atacatccaa ctggaagctt aggaaagata
     9481 gaagatattt gcatgcaatt agcaggaata tttggcaatg aaaattttga cacaagtaaa
     9541 aaggttataa ttgcttttgc gggagaccat ggtgtatatg aagaaggcgt agcacctgac
     9601 cctcaaaata taacaaaatt acagtttcca aatttttcga aaggtctatg cggagttgga
     9661 gtaataagta aatttgtagg ggcagatgtt gtagctgtag atgtaggaat aaactgtgat
     9721 gaaaaactgg atggggtatt agactacaag ataagaaaag ggacatcaaa tatggcaaaa
     9781 ggtccagcta tgagtaaaca agaagcaatt agatgtttag agataggtat tgaaatagca
     9841 gagcagtgta tagaaaaaga ttataaggtt attggtattg gggagatggg tatagcaaat
     9901 actactccaa gtacagcaat aatatctgta atttcagggt gtgacccgtt agaagttact
     9961 ggtataggtg caggtcttaa gaaagaaaga ctaaagcata aggctgaagt aattagaaag
    10021 gctattgaaa ttaataatcc aaatccaact gatggagtag atattttatc taaagtagga
    10081 gggtttgaaa taggctcaat ggcaggtgtt atattaggtt gtagtgctaa taggattcca
    10141 gtagtaattg atgggtttat ttcttatgca gcagcattaa ttgcatataa gataaatcca
    10201 aaaactagag aatatatgat agcatctcat ttatcagcag aatcaggaac taaaagagca
    10261 ttagacatat taaaattaga ccctctatta aatatggata tgagattagg tgagggaagt
    10321 ggagctgctt tagcatttga tataatagaa gcttcaaact atacttataa aaatatggca
    10381 acttttgatg aaattgacat gggaagataa atataaaaat ttaatatgta ggagtgtttt
    10441 gatgagtaaa attattctag taacaggagg agctaggtca gggaagagta actttgcaga
    10501 aaaactatgt tttgacagaa ataataatac agcttatata gcaacctcta ttccatttga
    10561 tgatgaaatg aaagatagag taaaaaaaca tcaagagaat agacctagaa actggaatac
    10621 ttatgaaata tatgaagata tatattccat aattaaggag atatataaag aacatgaaac
    10681 tgttatacta gattgtgtaa cattattagt aaataatcta atgtttgaac acaatctaaa
    10741 tatagaagaa tctagtcaag aagaaataaa caaattagaa caatacataa aagaacaagt
    10801 tgtcaaactt attgaagaaa tagaaaaaac aaatctatac tttgtatttg taacgaatga
    10861 gttaggtatg ggaattgttc ctgggaataa acttagtaga atttatgctg atattgtagg
    10921 tcgtataaat caatatatag cttcgaaaag taatgaagtt tattttgtag ttagtggcat
    10981 agctatgaag ataaaggagt agtatgcaat tatgaaaaga tttattttaa tactacagtt
    11041 tttgacaagg atacctataa aattaaatgt aggatttgat gatgaatttt ataagtcaat
    11101 agtatatttt ccacttgtag gttttgtcat aggtatatta tcctatttaa taggatggat
    11161 ttctatgctt ttatttgaac catttatagc ttctataata ataactctag ctggtgtctt
    11221 aattactgga ggacttcata tagatggatt aggagataca tttgatgcaa tatatagtta
    11281 tagggataaa gaaaagatgc ttgaaataat gaaagattca agattaggta caaattcact
    11341 tctagcaatt atgtttgtac ttttattaaa ggttggattc gtttacgata taataagtaa
    11401 taattcattg tgggttataa tttttatgcc aatgattgca aggttaggag taatgttatt
    11461 gacatataaa actgtgacac caagagaaaa aggaatggga aatttattta taggaaaatt
    11521 aactacaagt atgttaataa ctgctataat atacacatta ttgatagttg ctcttattac
    11581 taaatttata tttttattac ctaatatagt gttgattaaa gtattaggtt cgattatagt
    11641 ggtttttgta tttataatat tattcaaaaa acatatttat aaaaaaattg acggagtaac
    11701 aggagatata ttaggctgtg gaatagaact ttcagagctt gtatatttaa tttacatata
    11761 tttgttaatt tttatgtttt tttaaagtaa aaagtggtga taatatgata agattaatat
    11821 taataagaca tgcactgaca aatgataata aaaaaggaag attatcaggc catataaata
    11881 gttgtattag tgaagagggc aaactacaaa taaataaaat taccagatat ctgagtaatg
    11941 aaaacataga taaggtttat acaacaccat ctacacgtac taaagataca gtagaaaaaa
    12001 tatcaaaatt gaaattatta gaaatagaag aaaaagaagc tttaagagaa attagttttg
    12061 gagactttga aggaagaact tttgaagaaa ttaaaatcaa atatccaaat gagtttgaaa
    12121 aaatgataaa agaaggtaac aactatagat atccaaatgg agaaagtttg atagactctt
    12181 ataaaagagt ggcaaaagaa attgataata taattttaga aaataatagt aatttagata
    12241 caaagacaat acttatatgt tctcatgcag gaacaataag aaatataata acacatttga
    12301 tttctggtag ttataagtat cactggaact ttaaaattga taatgcatct attacagttt
    12361 tagaaattga tggtggattt gctgtaatag ataaaatgaa ttttacagac ttcatataat
    12421 ttaatcaaaa ccaaacatta caatctgtgt ttgtaatata tctgtaaaat tagcaatgaa
    12481 aatagaatat tagattaaaa ttaggttctt aattagatgt taaaagggaa aaaggttaga
    12541 tgcctttgca gcccccgcta ctgtgaaacc aacgaaacta taaataccac tgtcagtttg
    12601 atgggaaggt tatagaagta ggatgaagtt aagccaggat acctgcctaa tttaatttaa
    12661 aacaagaggt cttcggggtg aaagattttt aattaatata tataaaagtc ctatctttag
    12721 aagtattagg acttttttta atttgttttt tagtaaattt taacaaggag ggatggaaag
    12781 tggggaaaaa aataatgcta caaggaacag cttcaaatgt aggtaaaagt gtcttagtag
    12841 caggtttgtg tagaatattt aagcaagatg gttatactgt tgctcctttt aagtcacaaa
    12901 atatggcttt aaattctttt ataactaaag aaggattaga aatgggaaga gcacaagtat
    12961 ttcaagcaga ggctgcgaaa atagagccaa tagctgatat gaatccagtt ttattaaaac
    13021 cttctggaaa taataaaagt caagttattg taagaggtaa agttgtagga gaaatgcctt
    13081 cttctaagta tcatgattat aaattggagt tagtagatgt gttgaaggaa acatttaata
    13141 gcttaaattc aaggtatgat attgtagtta tggaaggggc aggaagtaat gcagagataa
    13201 acttaaaaga tagagatatc tctaatatgg gtatggcaga aatagctgat gcacctgtaa
    13261 taatagtcgg agatattgat agaggaggcg tatttgcctc attagcagga acaatgcttt
    13321 tattaaatga agatgagaaa agaagagtaa aaggtgtaat aatcaataaa ttcagaggaa
    13381 aaaaagagct tttacaagat ggtgtaaaga tgctagaaga catcataaaa gtacctgtta
    13441 taggagtagt accttataca gatataaaaa tagaagatga agacagtgta actacgagat
    13501 ttaagaaaaa aatgaataaa agtgatatac acatagaaat aattagaacc ccacatatgt
    13561 caaattttac agattttaat atatttgaga cacaagaaga tgtaagtata agatatgtag
    13621 actatggaga atctttagga aatccagata tattaataat acctggaact aaaagtacta
    13681 tagacgattt aacatatata agaaaaagtg gtcttgaatc gcaaatcaaa aatttacata
    13741 gtcaaggtaa attgatattt gggatatgtg gtggatacca aatgttaggt aaaaaattaa
    13801 aagaccctta tcatgttgaa agtgaaattg aagaagttga tggaataggt cttttagata
    13861 cagaaacaac gtttgagaaa gaaaagacta caacacaagt agaagctact attgttgaat
    13921 gtatcagtga atacatgaat aaccttaagg gaaaaaaagt aaaaggttat gaaattcata
    13981 tgggaattac aaagcgaggt aatgatgtaa gaagtttaga tttaataact aaaaaacttg
    14041 ataaagaagt gaattatact gagggaagtg taaataaaga ggggaatgta attgggactt
    14101 atcttcatgg aatatttgat gaaataagct ttacaagagc aattttaaac aatataagaa
    14161 agaaaaaagg tttaggagaa ttagaaagca aagtaaactc ttttaatgaa tttaaggata
    14221 aagagtatga taagttggct gattttttaa gagaacattt agatatagag aaaatatatg
    14281 agattatgaa ataatgatga cctttattta taatttaggt aggaggtgta ttatgaaaaa
    14341 aattttaatt gcgggtacta atagtggagt aggtaaaaca acaatatcat taggaataat
    14401 gcaagctttg gttaagagaa atatgaaagt acagccatat aaagtagggc cagactatat
    14461 agacccttca tatcacactt ttataactgg aagacattct agaaatttag attcttatat
    14521 gttagatgat gaaaaaataa agtatatagt aaataagtcc tcaaaagatg cagatatttc
    14581 agtaatagaa ggtgttatgg gcttatatga tggctttgga atagatttag ataattgtac
    14641 aagttcacat acttcaaagg tattaaagtc acctgttata ttagttataa atggtaaatc
    14701 tatggcagca tctagtgctg ctatggtact tggatataag gaactagata aagatgtaaa
    14761 tataaagggt gttatagtaa ataatgtaaa gactaatagc cattatcaaa taattaagag
    14821 ttctatagaa aaatattgta atgttgaagt attggggtat tttcctccaa ataacaaatt
    14881 ctctttggaa tctcgacatc taggattagt tcctagtgta gaaatgaaat cactaaggga
    14941 aaaattttat accttagcag atgaaattga acactatata gatattgaca gaataattga
    15001 aatatcagaa agtgatgaat ttgagagtga ttttgatttt caaactttga tagaaaataa
    15061 taaaataaaa gaacatgtta ataataaatc aatagctata gcatatgata aagcattcaa
    15121 tttttattat agagaaaata tagagctttt agaggaactt ggtttagaaa taaactattt
    15181 tagccctctt gaagatgtat cagtgccaaa tgcagattat atttatattg gaggaggttt
    15241 tccagaaata tttggaaaag aattagaagc aaatgagtca atgagaactt ccatattaga
    15301 agcacataac aataatatac ctatttatgc agaatgtgga ggactgatgt acctaggtga
    15361 gaaactattg aatcaagaag aacaagaatt taatatggtt ggaatattta gtggaattag
    15421 taagatgaca tcttctttaa agagatttgg gtactgtatt ggtgtagcca aggaaaatac
    15481 tcttttatct aaaaaagggg aatccattaa agggcatgag tttcatcatt caatatttga
    15541 aagtgatgaa aaatgtgtat attcaatgag aaaagaaaga gatggaaata tagtagaaga
    15601 gtgggatggt ggttatagta aaggaaacac tttggctaca tatcttcata ctcattttta
    15661 taataactta aattgtatag aaaatttttt gaaaaaagga aatgaataaa tgagtatttt
    15721 atctatttat ataggatatg tgacagactt aattgtagga gacccatatt cttttcctca
    15781 tccagtgaga tttattggta aacttataaa ttttactcaa ggtataataa gaaaggtctc
    15841 taaaaatgat aggcagttaa aatttggggg atttatatta tggtttataa ccgttggaat
    15901 aacatattta acaacttatg ctatagtgaa gttatttagt tttaatatat tattgagtat
    15961 tgtagtgaat agttttataa tttatacaac actagccaca aaatgtttaa aagacgaagc
    16021 attaaaaatt tataatgtat taaaaactga agatatagaa aaatctagga ttcagctatc
    16081 ttatatagta ggtagggata ctactaattt aagtgaacct gaaataataa gagcaactgt
    16141 agaaactgtt gcagaaaata ctgtagatgg tataattgcg cctatgtttt atgcatttat
    16201 tggaggagca ccacttgcta tggcctataa agctatcaat acattggact caacagttgg
    16261 atataaaaat aaaaagtata aagatattgg ttttgcatct gctaagattg atgatatagc
    16321 caattatata ccagctagaa tatctgtcat tcttatgaca attggaagtt tctttttaaa
    16381 ttataactat aggaactgtt ttaaaatctc tattagagat agaaagaatc ataaaagtcc
    16441 aaattgtgct ttttcagaag gtgcagtatc tggtgctcta ggaatacaac taggaggtac
    16501 caacgtttat tttggggaaa aagtatataa gccaacaata ggggataagc ttagggagat
    16561 agataaagaa gatataatta aaactaataa aattatgtat gcatcttcat ttgtatctat
    16621 gttggtattt acactaatat atgtgttatg taggatgatt ttaattaact taattatata
    16681 aattattgtt tgttaactta agggggacat agatgaagga tttagggcat ggtgccaatg
    16741 tagatgaaat ggcaagatta tatgggaaaa acccaaaaga aataattgat tttagttcaa
    16801 acataaatcc aaatgtatta ccaaatttag agagatacat tttaaaagga ttagaagagt
    16861 gtaggaatta tccagatata aattatacaa acttgagaga aaatatatca aaatatatag
    16921 atataaatcc agactttata atacctggca atggtgcaac agaagtgatt tatttgctta
    16981 tgaagagtat taaaaaaaaa ttggctataa taaatccgac tttttcagag tatagaagaa
    17041 gtgctgagtt aaataattta gatattatag atttagagtt agatttagaa aataacttta
    17101 aacttaatat agatataata aaagaaaata taaaaagatt tgatagttta tttatatgta
    17161 atcctaataa tccgagtggt aatgtccaag atttaaagga acttgttcat ttactagata
    17221 aacataataa ggtactaata attgatgaaa ctttcatgga atttgttgaa gatgaaagta
    17281 aatatagttt ggttaaatat atagaatcaa ataaaaatat atttataatt aaagctgtga
    17341 ctaagttttt tggtatgcct ggtttaaggc taggatatgg tcttacaagc aacactgaaa
    17401 ttatgaacaa aatttatgaa cacaaagagc catggactat caattctttt gcagatatac
    17461 tatccaattt tatttttgaa gataaagagt atattaaaaa tagtaaagaa tattacatag
    17521 aagaaagaaa atatatgtta caagagttga gaaacataag aaatataaag gtctatgata
    17581 cagatgctaa ttttatcttg ataagaatat ataaaaaaac aactaaagaa ttgaaaaaag
    17641 atttgtttaa gcaaggaaac atattagtta gagatgcatc taattttata ggattagatg
    17701 atagctttat aagagtggct ataaaatctc atgaagataa caaaattctt atagaaaata
    17761 tgaaaaattt attgggtgat taaaatgaaa tcttatggaa tatgcccagc atcttgtgga
    17821 gaatttgtac aaggtataat tgatgatgaa gaatatttat gttcatatgc aatagatatg
    17881 tattctaaag tgtatattga agaaaaatta gtagatataa atttagggag atataagtca
    17941 aggttagcaa ttgaaaaggt atttgaaaaa tttaatctac ctaaaaaata tactaaaaat
    18001 atttctttaa atataaatag taaaatacct gttggaaaag gaatggcaag ttcaactgct
    18061 gatataggag ctactataaa ggcaacatta tcattgatag ataaagattt aagtagcgag
    18121 gagatttcta aattagcagc agaaatagaa ccaacagatt ctatatttat agataaaaat
    18181 agtatattta atcctctaaa tggaactgtt ataaaatacc taggaaatct gaccaatgcg
    18241 aaagttgtaa tacttgaacc aaacaaagta ctagatacta tgaaaattag attgaggcaa
    18301 gattataata aattaaaagt agagaataaa gaagtaataa aaaaatcatt tgctctttta
    18361 gaagaaggat tgaaaaaaaa taatttatct ctagttggag aggcttgtac tttgagtagc
    18421 ttagcaaatg agaatataga aaaaaaagag tatttaaatg aaataataaa aatatcaaaa
    18481 aaatatggag catatggagt aaatattgct cacagtggaa ctgtagtagg tattttaatt
    18541 gataagttta tgaatgataa aaagatgata gatgccttat gtgaatctaa cataaattct
    18601 gtctataata aaatttatac acaaaacata attaatggag gaattaaggg ggaaatagaa
    18661 tggaatacat aaagaatcct atgaaaatag aagaaaaaag ttttgaattg atacaagaaa
    18721 taatagacga aattagacca gattataaat ttaagaataa tatcgaagaa aaaataataa
    18781 aaagagctat acatactact gctgattttg attacttaga tatattaaaa atatcagaag
    18841 aagcagttga tagtataata gatgcattaa aaaataatgc aagtatttat actgatacaa
    18901 atatggctct tagtggtata aataaaagaa agttagaatc attaggatgt aagtataaat
    18961 gtttagtggc agatgatgaa actgtaaaat tagctaaaga aaaaggaatt actcgttcta
    19021 tggcagcagt agaaattgca gctaaagaag aaggaagaaa aatattcgtt ttaggtaatg
    19081 ctccaacagc tttatataaa gttatggaga tgaaatcaga aggtagatta gatttagatg
    19141 cagttatagg agttcctgta ggttttgtag gagcacaaga atcaaaagat gaagttgaaa
    19201 aaacagatat accatatata atttctaaag gtagaaaagg tggcagtaat ttagcagcag
    19261 caatagtaaa tgctatatta tatactatgt aggtacctat atggaagaat atgtatatat
    19321 agatggaaaa aaatatagaa gaggatatac tacaggttca tgtgcaacag gtgcatctaa
    19381 ggctgctgta tatatgttga ttacaaaaaa tagaatcaat actataaata tagatactcc
    19441 taaggggata ccattactac taaaagttga taatataaat atttctgata catttgtgga
    19501 atgttccata aaaaaagatg gtggagatga tatagatgca actcatacca tggatattta
    19561 tgcaagagct gaaattgtag ctaaaaatga taagaacaaa gggtatctta ctctaaagga
    19621 tatagataat ttaagcacca atagtgagtg taaaagtgag ctctataagt ttattagggt
    19681 ttatggcgga actggtattg gtgtggttac aaaaaaaggt cttagcgtgg atgttggtaa
    19741 acctgctata aatccgactc cacttaaaat gataaatcat gaaataagaa agcttatagg
    19801 agataatttt gaatctatac ttggaaatga taaagtttta aaaataacaa tctttgctcc
    19861 acaaggagaa actgtagcaa aaaaaacatt taatccaaga ttaggtatag ttggaggaat
    19921 atctataata ggtacaactg ggattgttga acctatgagt gatgagggtt ggaagaagtc
    19981 tctatctata gaacttcaga tgaaaaaaga acaaggctta gataaaataa tattggttcc
    20041 aggaaatcat ggagaacagt ttataagaga aaaattaaac ttagatatta agtatgtagt
    20101 tagggtaagt aattttatag gctatatgat taaagaagct caaagaatag gatataagaa
    20161 aatcttaatg gcaggtcata taggtaaatt tataaaagta tctgctggta tttttaatac
    20221 tcatagtaag gtagcagatg ctagaagtga gatattagtg gcaaacttag ctttaatggg
    20281 agctagatac gaatttttaa ataaaataaa tcaatgcgta actactgaag aagctgtaga
    20341 gctaataaat aatagtgaat atagagaagt atataatata ttaagtaata aatgtaggga
    20401 gagagtaaag caatacttga atgaagattc agatgatatt gatgtggaag taatcatatt
    20461 ttcaatggat aagtctttat taggtaaatc ggataataca gatgatttag tggaggtctt
    20521 tatatgataa acataatagg tctaggacct ggtaacttag actatataac aaaaaaaggt
    20581 gaaaatttaa tttccacttc tgatgtatta attggtggta aaagaaattt agagtctata
    20641 aaaaattttg aaggagaaaa aatagttctg gattctaatt taagagaaat tatagagtac
    20701 ataaataaca ataaagaaaa gcaaatatca ataatagcat ctggggaccc actaatatat
    20761 gggataggta gatatctatc taaaaatatt gataataaga tgttaaacat ggtttctggg
    20821 attagttcat tacagtacat attttcaaaa atatatgtag atatgaatga tgtatatatt
    20881 acaagtagtc atggtaaagt accagatttt gattacatat tatcacataa aaaagtatgt
    20941 atggttacag attcaaagat aggaccaaaa caaataagta gagaaataat agatagaaat
    21001 ctaaataaaa ttattgttgt aggagaaaat ctgtcatatg acaatgaaaa aataacaata
    21061 gcaaaaccag aagagataat aagaatggac aattttgata tgaatgttgt ggtgatttta
    21121 gatgaggaat agtgaattta taacaggtaa agttccaatt actaaggaag aggttagagc
    21181 aatatcaata agtaagttaa atttagtaaa tgctaaaaac ctcatagaca taggggctgg
    21241 tacaggaagt gtaagtgttg aagctgcata taattatcct aatttaaagg ttatatctat
    21301 agagaaaaat gatgatgcta tagagcttat agagaaaaat aaaaaaaaat tcaacttgaa
    21361 aaatatagaa gtgataaaag gatatgctcc aattaatcta gggttaaatg ttaaagttga
    21421 gtctattttt cttgggggaa caggaaataa tctggaagaa ataatagaat ggtctaaaaa
    21481 aaatctcgtt acaggaggaa gacttgtagc taattttata ataatagata catttaatca
    21541 aactttaaaa ctgcttaaaa agcatggatt taaagaaata gatgtatgtg ttttgaatgt
    21601 ttctaaacta gagaaactag gaaaaggtga atattttaaa ccactaaatc ctatatatat
    21661 aatatcttgt gaaaaagggg aaaatgatga tgaataaagt acattttgta ggagcaggac
    21721 ctggagataa agagctgata acattaaaag gatacaaatt attaagtaat gcagatgtag
    21781 tgatatatgc aggttcactt gttaatcctg aacttttgga atactgtaaa gaagattgcc
    21841 aaatacataa tagtgcacat atggatttac aagagataat agatgttatg agagaaggta
    21901 tagaaaataa taaatctgtt gtaagactgc aaacaggtga tttttctata tatggttcaa
    21961 ttagagaaca agtggaagat ttaaataaat tgaatataga ttatgactgt actcctggag
    22021 taagttcttt tttaggggca gcatcatctt taggggttga atatacagtt ccagaaattt
    22081 ctcaaagtgt aattataaca agaatggaag gcagaactcc ggttccagag aaagaatcaa
    22141 tacaatcata tgctaagcat cagacatcta tggtaatatt tttatctgtt caagaaattg
    22201 agaaagttgt gtctaaatta ctggaaggtg gatatccaaa agatactcct atagcagtta
    22261 tatacaaagc aacttgggca gatgaaaaaa tagttaaagg aactttaagt gatatagctg
    22321 taaaagttaa agaaaacaac ataaataaga ctgctttaat aatggttggg agatttttag
    22381 gagaagagta taataattca aagttatatg ataaggattt caaacatgaa tatagagggt
    22441 aatattgcta taatctgtat tacagaaaat ggaaaaaaat tagctctaaa gataaattct
    22501 ctagtaaata actgtgttat atatcaagta aaaaataaaa aaagtaattt agttgttgaa
    22561 gaaacatgtg tgcatacagt tcaaaagaag ttaagtgatt ttgtaggaga aatatttagc
    22621 aaatttgatt acatagtatt tataatggct acaggtatag ttgtacgaag tatagcacca
    22681 tacataataa gtaagtttag tgaccctgca attttggtaa tggatgaaaa aggcaaaaac
    22741 gtaataagtt tattgagtgg tcatattgga ggggcaaaca gcttgacaca gtacataagt
    22801 agtcttattg atgctaatcc agtaataaca acagctactg atataaatca aaaagcttcc
    22861 ttagatatga tagcaaaaaa actaaatgca catattgata attttagaga aagtgtaaaa
    22921 gaagtaaatt ctatgctagt gaataataaa tgtgttggat tatatgttga tgaaggatat
    22981 caagttgata ctaggggatt taaacttata gaaagtaagg atgagttaag agatagtttt
    23041 tcaaacacat atatagaaaa attagttgta gtaagcaata aaataaattg tattgaagat
    23101 attgagaatt taaacctaga taataaattt gaacattgct atagtaccca aaaaataata
    23161 aaggtaattc caagagatat agtgatagga attggctgta gaagaaacac agatagtgaa
    23221 ctgatgtata attcacttga agagtttctt aataaacaaa atatagatat tagagcaata
    23281 aacacagttg gaagtataga actgaaaaaa gacgaaaaag caataattga attatcaaaa
    23341 atattgaagg tgccatttaa aacattttca gtagatgaaa tatctaaggt tgataatctt
    23401 tttgataaat ctgattttgt taaaaaaaat gtgggtgtgt attcagtagc agaacctgta
    23461 gcatatcttt tgagcaaagg taatttgtta gttgaaaaac ataaatataa aggaataact
    23521 ttttcagtgg ggagaataaa aatatgatat acgttgtagg aattggacca ggaagtaaag
    23581 atacaatgac tttagaagca ataaaagcaa tagaagattc tgaggctatt gttggatata
    23641 agacttatat aaaactaata gaagaattta tacaaggtaa agaaataatc caaaatggta
    23701 tgaggcaaga agttgataga tgtaaacaag cagtggagga agcaaaaaaa ggaaaaaaag
    23761 tagcagttgt aagcagtggt gatgctggaa tatatgggat ggcaggtctt attttagaat
    23821 taatatctaa agaagatgaa gatataaaag taaaggtaat tccaggcgtt actgcaagta
    23881 ttggtgcagc agctatactt ggagctccta ttatgcatga tttctgtcat ataagtttga
    23941 gtgatttaat gactccgtgg gaagtaattg aaaaaagatt gagattggct gctgaagccg
    24001 attttgtaat ttgtttgtat aatccaagaa gtaagggaag aagtgaacat ctaagcaaag
    24061 cattcaaaat aatgggtgag tttaaaggtg gagatacacc tgttggagtg gtaaaagatg
    24121 taggtagaga aaaagaagaa aagtttgtat gtacttttga aaatatggac tttgaaaaag
    24181 tcgatatgac tacaatggtt ataataggaa ataagtctac ctatattaat ggtgaattga
    24241 tgataactcc aagaggttat acagtatgat tttaatttta ggaggaactt cagatagttt
    24301 atctatatgt gataagataa atgaactgaa aaatcaacca tatatccttt cagttactac
    24361 agattatgga agggaacttg cacaacaaca tgcagagaat gtaattttag gtaagcttgg
    24421 aaaagaagat atgctaaaat ttataaaaga taataatata gtaaaaataa tagatgccac
    24481 tcatccttat gcagttgaag tatccaaaac tgctattact tgtgcaaaac tattgaatat
    24541 agattatata aggtttgaga gaaaatcttt aattgaagaa atagattatg ataataaatt
    24601 tatagtagat acaattgaag aagcctgtga aatagcaaat aaaataggga caaatatatt
    24661 cataggaaca ggaagtaaaa atttgaacag atttatagag gaaataagtg ataaaaattt
    24721 agttgttaga gttcttccaa cgagtgaagt catattaagt tgtgaaaaat taggtttaag
    24781 tgcagataat atagtagcta tgaaaggtcc atttagccag tatataaatg aagaaacata
    24841 taaacattac aatatagatt tagtcataac aaaagaaagt ggaatagctg gtgggttctt
    24901 agagaaggta aatgcatgta aaactttcaa tataccagta ataattataa aaagagaatc
    24961 gattaactac gaaaatgtat ttaatgacat aaataaaatt aaagacatac ttgtgtaatt
    25021 tttaggagga gaatatatga agaaagcctt attagtagtg agttttggta caagctatca
    25081 tgaaacgaga gaaaagacaa tagatatatg tgaagataaa ataaaaaatt cgttaaaaag
    25141 ccatgatttt tttagagctt atacatcaaa tatgataata aataaaataa aaaaaaggga
    25201 tggaatagaa atagataatc caatacaggc tttagataaa atatatgaac aaggatatga
    25261 tgaagtaata atccaaacac ttcatattat atgtggcgaa gaatttaaca aattaaaaga
    25321 acaagtagat ggatattctt ctaaattcaa aaagattgta ttaggaagac ctttgcttac
    25381 acatattgaa gattatcagg aagctgtaga ggcaattaag catcaaattc ctcatatgga
    25441 aagtaatgaa gcagtagtat ttatgggaca tggaacactt catgaatcac attcagcgta
    25501 tccagcattg gaatacatgc ttagggataa tggaataaat gcttatgttg gtacagttga
    25561 agggtatcca gaaatagaac atgtaataag aagattaaaa gaaggaaata taaaaactgt
    25621 aaatttgatg ccatttatgt tagttgcagg agaccatgct ataaatgata tggcaggaga
    25681 tgaagaagat tcttggaaaa caatattaga agaaaatgga tttaacgtaa gagttcattt
    25741 aaaaggacta ggagaaaatt cttatataca agataaattt gttcgtcatg ctgtaaaatg
    25801 tgcagagaat gcgaagttct atggtatagg agcagggcca ggagatggtt ctttacttac
    25861 gataaaagcg gttaatactt taaagaaact ggatattcta tacactccag aatcaaaaaa
    25921 gggtggagat agtctagctt tatcaatagt tagtgaatat ttacctgaat cagtggaaat
    25981 aaagtctagg catttcccaa tgagttttga tgggaatgaa aaaagtttag cttgggacaa
    26041 tgtggcacaa gaaattatat cagatgttaa tgatggtaaa aatgtaggat ttgttacatt
    26101 aggagaccct atgatttata gtacatatgt atatataatg aaaagaatat taggagaggt
    26161 agaagtcgaa actataccag gaatttcatc attttccaat atagcatcta atcagaattt
    26221 tccattagtc atggataaag aagctttagt ggtaattcct tgtactatgg aagatgataa
    26281 gatagagtat gctttgcaaa attataattc aatagtattg atgaaagtat ataaaaattt
    26341 taaagaaata ataaaaaaat tagaaaagta tgatttaata gaacatgcta ttttagtaag
    26401 taattcttcc caagaaagcg aaaatgtgtt tacagattta aaagaagcgc accttgaaga
    26461 taagatatct tatttttcta ctatactagt aaataaagat aataaaataa aataggtgaa
    26521 agcaatattt aaaggagact taatatgaat atagtggttg gaactagggg tagtaatcta
    26581 gcgcttatac aaacagaatg ggtaataaat gaattaaaaa agaagtatcc agaaattagt
    26641 tttgagatta aaatcataaa gacaaaagga gatttaattc aaaatgtttc actggataaa
    26701 ataggtgata aaggattgtt tgtcaaagaa atagagcaac aattattaga tggaaaaata
    26761 gacatagcag tacatagtat gaaagatatg ccttcttatt tagcagatgg actaaaattt
    26821 gctcatacgc ctaagagaga agacccaaga gatgttctaa tacttagaga aggatataaa
    26881 aatttagatg atttgcctca tggagcagta ataggaacag gaagtaaaag aaggaagttt
    26941 caattattga aacaaagacc tgatttaaat atagtacaag ttagaggtaa tgtggaaact
    27001 aggatacgaa aaataaaaga tgaaaacatg catggtattg tgttggcagc atcaggaata
    27061 attagagcta atttacaaga taaaataagt tcttatttac ctgttgatgt tgttattcca
    27121 gcacctgccc aaggagcttt agctatagaa attagaagca atgatagtac gatagaggga
    27181 atagtaaatt cactgaagga tgagaataca gaaatccaaa tattggctga aagaggtttt
    27241 ttagatggtg taaatggaag ttgtcatatt ccaatggcag cttattgtga aataatccaa
    27301 gataaaatac atcttactgg cttatatgga gattcagaag gaaaaaaagt agtaatcaag
    27361 tctatagatg gagatatttc ttctcctaga gagttaggtt taaagttagc taaacttgta
    27421 ttgaaggagt atgagaatta tgaagggtaa ggtttactta gtaggggcag gaccaggtga
    27481 ttataaactc atgactttaa aaggtttaga gtgtataaga aaatcggatg taatagtata
    27541 tgatagatta gcaaatagta gttatttaag agaagctaag cctgattgtg aacttatata
    27601 tgtaggcaag gcatcaagta atcatatttt gccacaagaa gatataaata gaattatagc
    27661 agaaaaagct aaagaaggaa aaattgtaac tcgtcttaaa ggaggagacc cttatgtatt
    27721 tggcagaggg ggagaagaag cagagacttt agtagatgaa ggtatagaat ttgaagtagt
    27781 tccaggtata acttctgcta taggaggttt atgttatgcg ggtattccta taacacatag
    27841 agaccatgct tcgtcatttc atgtaataac aggtcatttg aagggtgatg atagtggaga
    27901 attaaactgg aatgctcttg caaataacaa aggtactctt gtgtttttga tgggaatttc
    27961 aaacctagag aaaataagtg aaaacttaat gaaagaagga aaagataagg atacacctgt
    28021 tgctcttata agttgggcaa ctagatataa tcaaagagtt gttacatcta cacttgaaaa
    28081 tgtttatgaa actgctatta aagaagaagt aaaaccgcca actcttatag tagttgggag
    28141 tgttgtagga cttagagaaa agttgaactt ctttgaaagt aagcctttat ttggaaaaag
    28201 tattgctgta actaggtcta gaaatcaaaa tagtgtattg gtagaaaaaa taatggattt
    28261 aggtggaaat cctatagaaa tacctactat taaggtagaa aaaatacaaa ataatattaa
    28321 cttagaaaat gaaataaaaa atataaataa gtataattat ctaatactaa ctagtaaaaa
    28381 tgccgtagaa atcttctttg aaaagatata tgaaatgaat tttgatttaa ggatattatc
    28441 taatttaaag gtatgtgcta taggttctgc tacatcaaat gagttgaaaa aaagaggaat
    28501 tatagcggat atagtaccaa aaaaatttgt agcagagtca ctgtatgaag agttagcgcc
    28561 tattttaaat gatgacgata atatacttat acctagagca gaaaatgcta gagattatct
    28621 ggttgacaaa ctcaaggcta tttgtgaagt aacagaagta catatatata gaacagtaat
    28681 agatgaggag aaaaaaggtg agatattaga tattttaaat tctaatgaca tagattatat
    28741 aacatttact agttcctcaa cagtaaataa tcttgttgaa attataggaa aagaaaatat
    28801 taaaatatta gaaaaaataa aaacactatc tataggtcct ataactacag agacaataaa
    28861 tagtttggga ataaaattgt acaaagaatc tgctacatct actatagatt cgttagtaga
    28921 tttaattata aaaaattaat tttggaggct tgaatatgtt aagaagacca agaagactaa
    28981 gagcaagtaa agcaatcaga aatttggtaa gagaaactaa ggtgaatgta gaagacttaa
    29041 tatatccact gtttgtagta gagggggaaa atataaaaga agaaattaaa tctttaccag
    29101 atgtctatca tttatcaata gatatgctag aagacgaaat aaaagaaata agtgagttgg
    29161 gaattgaaca tataatgtta tttggtgttc cagatgaaaa agatgagtgt gcttcacaag
    29221 cttttaatga taatggaata gttcaaaagg caattagaaa ggtaaaagaa ataaattcag
    29281 atataaatgt aataacagat gtctgtatgt gccaatatac aagtcatgga cattgtggga
    29341 ttttaactga tactggttat gttgataatg ataaatcatt ggagtatttg gctaaaatat
    29401 ctgtaagcca tgctaaagca ggagctgata tggttgctcc atctgatatg atggatggaa
    29461 gaatacaagc aataagagaa gctttagatg aaaatggatt agaaaatgtt ggaatcatgg
    29521 cttatagtgt taagtatgct tcaaattatt atggaccttt tagagaggca gctcattcag
    29581 caccatcttt tggagataga aaaacatatc aaatggaccc agcaaatagc aatgaagcta
    29641 taatagagac tgaacttgat atattggaag gtgcagatat acttatggtt aaacctgcat
    29701 tatcatattt agatattata agaagagtta aagataattt tgacttacct ctagcagctt
    29761 ataatgtaag tggagaatac tctatgctta agtttgctgt gaaggaaggt ttattaaatg
    29821 aagatgtaat tcttgaatca attacatcta taaaaagagc aggagcagat ataataataa
    29881 cttattttgc taaagacatt gctaaactgt tgaaaaaata aattctagca gtataaagaa
    29941 tatcaaaaag tataggaatt gtaattaact attatacagt agttttgttt agacatttat
    30001 tgaagaataa tagaaactaa tttgcaattt ctatacttta tttatgtaac taaataaaat
    30061 ttggaggtag aagatgctat atcctattaa tttaaaatta gatgagttag atgtagttat
    30121 aattggtggt ggagaagttg catataggaa atgtaaaaat tttttagagt ttaacaaaaa
    30181 tgttactata gtttctaaac aaattttaaa taaattttat gatttaaaag gaaatataaa
    30241 aataataaaa gatgattata aagaaatata tataaaagat gcttctgtaa taattgctgc
    30301 cacaaataat agagagttaa atatggaaat aggattatat tgtaaagcaa aaaataagct
    30361 agtgaatgta gttgataata tagaaatatc gaactttact gttccatcat atataaaaag
    30421 aggagattta cttataagtg tgtctactgg gggcaagagt ccttcattat catcaaagat
    30481 aaaaaaagaa attgaagaga gatatactga agattatgag gagtatttaa atgtattagg
    30541 agatataaga aaaaaggtta taaaaaaata cgaagataaa agtaaaagaa aagatgtgct
    30601 taatatgttg gtaacacttg acctagaaga acttaagaag tttgatatat aaaaaataaa
    30661 ataaaaaatg ttaaaaaggt gttgacggat
//
LOCUS       DAMJYX010000035        30564 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJYX010000035 DAMJYX010000000
VERSION     DAMJYX010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30564
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.035"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            373..1239
                     /locus_tag="PC479_001762"
     CDS             373..1239
                     /locus_tag="PC479_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2804310.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1481..2290
                     /locus_tag="PC479_001763"
     CDS             1481..2290
                     /locus_tag="PC479_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804311.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2434..3498
                     /locus_tag="PC479_001764"
     CDS             2434..3498
                     /locus_tag="PC479_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2804312.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3526..4245
                     /locus_tag="PC479_001765"
     CDS             3526..4245
                     /locus_tag="PC479_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2804313.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4269..4853
                     /locus_tag="PC479_001766"
     CDS             4269..4853
                     /locus_tag="PC479_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2804314.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            4855..6204
                     /gene="pabB"
                     /locus_tag="PC479_001767"
     CDS             4855..6204
                     /gene="pabB"
                     /locus_tag="PC479_001767"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2804315.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6194..6937
                     /locus_tag="PC479_001768"
     CDS             6194..6937
                     /locus_tag="PC479_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2804316.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            6971..7672
                     /locus_tag="PC479_001769"
     CDS             6971..7672
                     /locus_tag="PC479_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804317.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7698..8270
                     /gene="folE"
                     /locus_tag="PC479_001770"
     CDS             7698..8270
                     /gene="folE"
                     /locus_tag="PC479_001770"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2804318.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8334..9125
                     /gene="folP"
                     /locus_tag="PC479_001771"
     CDS             8334..9125
                     /gene="folP"
                     /locus_tag="PC479_001771"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2804319.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9136..9501
                     /gene="folB"
                     /locus_tag="PC479_001772"
     CDS             9136..9501
                     /gene="folB"
                     /locus_tag="PC479_001772"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2804320.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9494..10000
                     /gene="folK"
                     /locus_tag="PC479_001773"
     CDS             9494..10000
                     /gene="folK"
                     /locus_tag="PC479_001773"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2804321.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            9981..10766
                     /gene="aroF"
                     /locus_tag="PC479_001774"
     CDS             9981..10766
                     /gene="aroF"
                     /locus_tag="PC479_001774"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2804322.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            11021..12811
                     /locus_tag="PC479_001775"
     CDS             11021..12811
                     /locus_tag="PC479_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2804323.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12829..13995
                     /gene="rpoD"
                     /locus_tag="PC479_001776"
     CDS             12829..13995
                     /gene="rpoD"
                     /locus_tag="PC479_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2804324.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14160..14849
                     /locus_tag="PC479_001777"
     CDS             14160..14849
                     /locus_tag="PC479_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2804325.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14863..15960
                     /locus_tag="PC479_001778"
     CDS             14863..15960
                     /locus_tag="PC479_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2804326.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            16023..16595
                     /locus_tag="PC479_001779"
     CDS             16023..16595
                     /locus_tag="PC479_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2804327.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16655..17122)
                     /locus_tag="PC479_001780"
     CDS             complement(16655..17122)
                     /locus_tag="PC479_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2804328.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(17508..17984)
                     /locus_tag="PC479_001781"
     CDS             complement(17508..17984)
                     /locus_tag="PC479_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2804329.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(18104..19195)
                     /locus_tag="PC479_001782"
     CDS             complement(18104..19195)
                     /locus_tag="PC479_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2804330.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(19188..19577)
                     /locus_tag="PC479_001783"
     CDS             complement(19188..19577)
                     /locus_tag="PC479_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2804331.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            19769..20215
                     /locus_tag="PC479_001784"
     CDS             19769..20215
                     /locus_tag="PC479_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804332.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20479..21204
                     /locus_tag="PC479_001785"
     CDS             20479..21204
                     /locus_tag="PC479_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804333.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            21191..22435
                     /locus_tag="PC479_001786"
     CDS             21191..22435
                     /locus_tag="PC479_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804334.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            22515..23192
                     /locus_tag="PC479_001787"
     CDS             22515..23192
                     /locus_tag="PC479_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804335.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23193..25754
                     /locus_tag="PC479_001788"
     CDS             23193..25754
                     /locus_tag="PC479_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804336.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            25975..27249
                     /locus_tag="PC479_001789"
     CDS             25975..27249
                     /locus_tag="PC479_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2804337.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(27410..30451)
                     /locus_tag="PC479_001790"
     CDS             complement(27410..30451)
                     /locus_tag="PC479_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2804338.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
BASE COUNT        12184 a         3281 c         5248 g         9851 t
ORIGIN      
        1 taattataaa gagacaatta ctaaacttga atttgctaaa ataaaataaa ttactgcgaa
       61 aatagatatt tagaaaaaaa tactataaat ttactatatt gcaagttaaa taataagcag
      121 tatcatatta tgctagaaaa agtttcattt aaggcataaa tgagatgatt tgaacgtttt
      181 taaagattag aatataaaag attgcactag acatatatga aaaagataat ataatagatg
      241 atgtaataga tgacagttca agaaattata aaaaaaattt atttgaaata ttatctatta
      301 catcaggaat aatatatgta agagtaagct tttaaaattc taaagattta gaaagattga
      361 agggagaaag aaatgagtga agttaagaat accctacaaa aacatccagt tttaagatat
      421 tcaacaatgc tatttgggat gttgatgaca tctataggaa taaatgggtt cttaagacca
      481 gcacatctac ttagtggagg tgctacaggt atagcaacat ctataaacta tttgacaaat
      541 ataaatgtag gtttattaac atttttaatc aatattccaa tatttatctt agggttcata
      601 taccttgaaa aagagttttg tatttcaagt ttggtaaata tgattgtatt ttcattatta
      661 ttaggagcaa ctcaggaaat aagtaatatt atacctatac atgatatatt gcttcaaagt
      721 gtatatggtg gtatattatc tggtttagga gtcggagttg tatttagaac taggtcttct
      781 caaggtggta cagatataat agctgcaata ctaaaaatta agaagaatat agaaatgaaa
      841 gacactgctt tagcaataaa tggcttgata gtacttacag gaagtttttt atttgggttg
      901 gatttagcat tatacactct tataggtcta tttctaaatg cgtattcaat gagttttatt
      961 aaagatgcaa tgaattacca aaaatctgtt atggtaatgt caaatgaagt cgatttaata
     1021 gcagaagata taatgaagag tttagttaga ggtgtaactt ttttagatgc agaaggtgca
     1081 tacactcatc aaaaaaagaa gattatatat acaatagttt catcaaatga gataccaaag
     1141 ataaaagata tagctttgaa gtatgataaa aaggccttta tatctgtaaa tgatgttaca
     1201 gaagtaaaag gaagagggtt taaagcaaaa gatttataga aaattttaca agctgggagt
     1261 ttataatgta aatttgtata ttatatctct cagcttattt aaatttagta tacgaaaata
     1321 aaggttttat taatgatttg tgtatcactt taaaagatat cattttaaaa ttaaaattga
     1381 aaaaattact taatatgtag aaaacatgac cactattagg atataataaa tttaatagat
     1441 ttagtaaaat atctaggaat aacgttggag gaatggcatt atgaatttat ctaaagaata
     1501 tgttaatttg tatgataatt atttgaaata tataaatgaa gtaaacaaag agattagaag
     1561 tataaagaac atgaagaaac gtgttttaaa aaggggcgat tttgctccag aaaatgaagt
     1621 attagaattg gaaggaaaaa caatacgcga tatagatgat gtcgatacaa aaaaaccaaa
     1681 aaataaaatc tataaatttt caaatgggga tgtatatgta gggaaattct tagagggaaa
     1741 gatggagggg aaaggctcat atactttttt tgtagatgaa ggaacagtta tggagtacat
     1801 aggagaattt aaagaagata aaaaaaatgg aaaaggaaac tttatatttt caaatggaaa
     1861 tgaatatata ggttattttg aggaagatat gatgagcggt attggaaata tgttatatag
     1921 tagtaaagat gaatatattg gaacttggaa aaacggaaaa aaagatggaa aaggtattta
     1981 taagtggagt gatggatgta tatatgcagg tgaatttaaa ggtggaaaga tggaagggta
     2041 cggtatttgc tataatagta aaggggaagt actttatgaa ggagaatgga agaataattt
     2101 gattcatggt aagggaactt atatatggga aaaaggtaaa aagtatatag gtgaatttat
     2161 gcatggtaaa aaacatgggc aaggaacttt ttatctaaat aatgaattgg tttatgaagg
     2221 gacttggaaa tttgataagc catctatatt tgatagaaca ttagatgaaa tattttcctt
     2281 gagactgtag ctttatctaa ttagtagata aagctttttt ctaatactta ctgtaaatag
     2341 gtatgaatga tattgattag tatacactat caatattaac ttaataaatg caatattaaa
     2401 aaattttgat tgattttaag aaggagagat atattgaaga gattaattgc tttagtgtta
     2461 gtagtactta ttgttttatt ttatagtcct gaattattaa aagaaggata tgatttaaaa
     2521 gaaaagtttt ttcaaagtga aatatggaat gaatttttag acaaaataaa tataaatgat
     2581 aattctaaga aagataaaaa agaaaaaagt caaaatttac aatcaaataa tactgaaggg
     2641 gagaacttag gagagtcaga tataaagaat tttgaaaaaa taagcttagg agataattta
     2701 agctatgttt tatcaagtat cggcaaacct ggtagaattg atattagtga atatggtttt
     2761 gattggtatg tatataatca atatggaaaa gaatttgcaa tggttggact tgaaaatgat
     2821 gaagtagtag cactttatag taattctatt aattcatgtg aaaatcaaga tataaaacta
     2881 aatcaagata gacaaacagt gagaactaag attacaccat taaaatataa gagaaaagga
     2941 aataccagat atataataaa ctcagaaaat caatatgata taataagtaa ggaaggtaag
     3001 tatattacaa ttttctacga tattcatgaa gaaaatagag tttgttcata tttaataatt
     3061 gataaaagca ctgaagatga gtttaaaaat ttatatccag atgatagcga agaacttaaa
     3121 aaatgttttg aacttgaagt tatagacctt gtaaatagtg ttagaaatca aagagggtta
     3181 aactcattga gatacagtga acaggcaact ttgagttcaa gaaaacatag tgaagatatg
     3241 agagataata atttttttga ccatgtaaat aagaagaatg aaactccatt tgacagaatg
     3301 aaaagagaag gtatagtata tacttcagct ggagaaaaca tagcagcagg acagattaat
     3361 gcaatatatg cgcatgaagc ttggatgaac tctgaaggac atagaaaaaa tattttgggg
     3421 aactataata atattggagt tggagtaata tttggaggaa gctataaaac atattacact
     3481 caaaattttt ataaataact aaaatatttg aaaggaagtt aaaaaatgac atcatggaaa
     3541 aaaaagacag tatataagtg cttaatagca gtagtcttgt tttgtggaat agtgttaata
     3601 tctaatttta gtaaagtttc tgctttgatg atggatacta atggaaatgt tttgattaaa
     3661 catggttcaa gggaaaaaaa gctaatagca attacctttg atgatggtcc tcatcctaaa
     3721 gaaactagtc aagttttaga tgtattaaag aaatacaatg taaaagctac tttttttata
     3781 gctggaaaac atgcaaaatg gtataaagaa ccattagtaa gagcaagtaa agaaggtcac
     3841 gagataggaa atcatacttt caatcatcct gatataagta atttaagttc tagtcagata
     3901 gaagaggaga ttgttaagtg tgaagacatt ttgaaagaag taactggaaa aaaaccgaca
     3961 ttatttagac caccatttgg aagttataga gaaaaagaat taatagaaat tgcaaaaaag
     4021 catgactata aagttgtttt atggacaggt gtagatgtga aagattggaa aaatccagga
     4081 gctaatagca ttgcagataa aataattaat aaagtacaaa atggagatat aatacttttg
     4141 catgattatg caactaatga tacagttgaa gctttagata tgtttattcc aaaaatgatt
     4201 gaaaaaggtt ttaaatttgt aacagtttct gagttaataa aataattttt aggggggaga
     4261 tttacagtat gattcttatg atagacaatt atgattcttt tgtatacaac ctagtccaat
     4321 atatagaaga attaggggaa acagttgtag taaaaagaaa taatgaaatc aaaataagtg
     4381 acattgaaga actaaatcca gaggtaattg tgttatctcc aggaccgtgt tctccaaaag
     4441 aagctggtat ttgcatagat attgttgagc attttaaggg gaaaaaacct atactaggaa
     4501 tttgtttagg tcaccaaact attggacatg tttttggagg agatattata aaagcacaac
     4561 agcctgtgca tggaaaagta tatagtatca accatactaa taaaggagtt tttaggggac
     4621 ttaaaaatcc tttaaatgtc actaggtatc attcactaat agttgactcc aatacagttc
     4681 caaaagaatt agaaattaca gcaattactg ataaagggga aataatgggg attaggcata
     4741 agaaatattt aatagaagga gtacagtttc atccagaagc tattttgtca gagtatggtc
     4801 atgaaatgct taaaaatttt ataacagaag ctagagaaag ggtacatatg taatatgata
     4861 agagagataa atactaaatt aaattctttt gagatattta ctatttttag aaatgaacat
     4921 gacagtttta tattagacag tgctatggac aaagaaaaat tgggaagata ttcttttata
     4981 agtagccaac catttaaggt attaaagtat aaagatactg atgaaaatcc tttagaagtt
     5041 ttaaaagaag aactacataa gtatagggtt gtaaatgata ctaatcttcc atttgtagga
     5101 ggtgctgtgg gatatttatc ttatgattta ggtaattaca tagaaaattt acctagaact
     5161 gctgtagatg atattgagat gcctgatatg tattttggat tttataatca tgtaatagtt
     5221 atagaccatc ttgtgcaaaa aacttatata gctactccaa acatagatat cgaattagaa
     5281 gaaaagataa tagatgatat agaacaaaga atattaaaag aagagaaaaa aggtatagat
     5341 agcatatgtt atgaagaaaa agaagtaaca cctataagac ttaaatctaa ttttacaaag
     5401 gaagaattta aaaatgcagt tcaaagtgtt agagagtaca taagacaagg agatatatat
     5461 caagctaatt taacacaaag gttcagtgga gaaacagaac ttacaagctt tgaattgtat
     5521 agggacttaa gaagatttag cccagcacca tttggggcat tcttaaactt tgaagatgca
     5581 catatattat caaattctcc agaaagattt ataagatgtg ttaataagag aatagaaact
     5641 agaccaataa aaggtactcg tccaagaggg aaagataaag aagaagattt aagacttcaa
     5701 caagaactta gaaatagtga aaaagataga gctgaactac ttatgatagt agatttagaa
     5761 agaaatgata ttgggagaat atctaaaact ggaagtgtta aagttccaga gctatttgta
     5821 attgaaccat atgcaaatgt aaatcactta gtttccacag ttgtaggtga gttaaaagat
     5881 gataaagatg ctactgatgt aataaaagca acttttccag gggggtctat aacaggagca
     5941 cctaagatta gagcaatgga aattatagac gaattagagc caacgcaaag aaatgtatat
     6001 actggttcaa taggttatat aggttttaat ggtgatatgg attttaatat agcaattaga
     6061 acaataatca aaaatgacaa aaaagtctat tttcaagttg gaggaggaat gacttgggat
     6121 tctgacccag atgaagaata tcaagaaaca cttgataaag ctaaatccat aatgaaggct
     6181 ttgaggggat attatgaaga ataatgtagg ttttgatagt agtttaagta aatttggaat
     6241 tggtttgttt gaaactatta gagttaaaaa tggaatagca gtagatttag atatccatat
     6301 agaaagaatg ctaagttcaa taaattgttt ggatttagat ataaaatata aaaaagattt
     6361 cttgataaat gaaattgtta catatataaa aaaagaaaat gtcattaata aagcacttag
     6421 aattactgtt tttgatgaag gttataatat atctattagg gatattccat ataatcaaga
     6481 gacgtatgag aaggggttta aattagctat atctccaata gttaggggga atagtttaat
     6541 tcatagacat aagactacta attattttga aaacatatat accaaaaata tcgcaaataa
     6601 gactggttat aatgatggta tatttttaaa ttcagaaggt gtaatattag aatgttctat
     6661 gagcaatatt ttctttgtaa aaggagaaag agtgtacact cctagtgata gattaccgat
     6721 attaaatgga ataataaaaa aaaggattat agagatttgt gacgaattac atatagagtt
     6781 gatagaaaat gaaattaata tatcagaaat tagtagtttt gattttgttt ttgtcacaaa
     6841 tagtcttatg ggcgctataa aggttacaga aattgacaaa ataaaattta ataaagaaaa
     6901 tgttgttttt gataaaatag tgaagttatt agaataaatt aaaatattaa caatttgttg
     6961 gagacaaaag atgataggtt gtatttatga agtatacaat gaagagataa ctaaaataat
     7021 agaaaaccaa aacactttgg ctgtatattt agtgggttct agtaaagacg tagattttac
     7081 tctttatgat gtagagataa acgatattga tgtgttcgtt tttgtaaaag aaggggaaaa
     7141 acaagagaga atactgttta aaaaaaaggg aatagaattt gatgtaaatt acttttcaaa
     7201 agatggtgta aaaaaattat tatcaaatag ggaatatttc tttgtgaaag aaatgaaaga
     7261 tgctaaagta ctgtttgata gggagaatat atcacatatt ataaaagata taagtagaaa
     7321 tatttactta gaaggaccca gtaaaatgtc attagaggaa aagtctttta taaagcaaga
     7381 tattggagca aaaatatcga atttaaaaaa taaagaaaaa tttgatgttt ttgaatatca
     7441 ttttttgacg aatttatatt taaaagatat aataattggg tactttaata taaatgataa
     7501 atgggtacca aaagacaaaa aattgctaaa agttttaaaa aaagaaaata atgagctttt
     7561 tgaactagtg tcaaaagtaa gtgaaaatta tgactatcaa agacttttag atgtttataa
     7621 ttatattttt aaagaaattg agacaaaaga ggttataaaa ctaatcttct agtcacaaat
     7681 caatgggggg agtttaaatg aataaagttg ataaagaaaa gatacaacat gcagtaagag
     7741 aaatactaga ggcaattggg gaagacccag atagagaagg tctaatcgaa actccaaata
     7801 gagttgctag gatgtatgaa gagatattct ctggtcttag tgaagaacca agagaccatt
     7861 taaaagttct ttttgcagat gaaaaacatg aagagttagt attggtaaaa gatattccat
     7921 tttattcatg ttgtgagcat catctagttc ctttctttgg aaaagctcat attgcttatt
     7981 taccaaaagg aggaagactt acagggcttt ctaaacttgc tagagtaata gatacattgg
     8041 caaaaagacc acaattacaa gaaagaatca ctaaaaatgc tgcagatata ataatggaag
     8101 aattacaacc ttatggtgtc ttagtagtag tagaagctga acatatgtgc atgacaatga
     8161 gaggcgtaaa aaaaccaggt tcaaaaactg ttacatcagc agtgagagga atatttgaaa
     8221 aggacattgc gtctagagcc gaggctatga gtttgattac tatgaagtag caatttatta
     8281 tagatataat catttattga gataaaattt aatttaaggg agtgtcatta attgtgtttg
     8341 attatggtaa aagaacttat ataatgggaa tattaaatgt aactccagat agtttttctg
     8401 atggaggaga ttttaataac ttggacatag caatacaaca tgcaaaagat atggttgacc
     8461 aaggtgcaga tataatagac ctaggaggag agtctacacg cccaggtcat agctatgtag
     8521 actcagacga agagctaaga agagtaatcc ctgtaataaa aaaacttaaa caggaattgg
     8581 atataccaat atcaatagac acttataagg cagatgttgc agaagaagca ttaaaattag
     8641 gtgtcactat ggtaaatgat gtttggggtc ttagaaaaga taaaaatatg gcttctgtaa
     8701 taggaaaata tgatgcagaa gtgtgtataa tgcataatca agatgggaca aactatgaca
     8761 aagatataat ggagtcaata aaagactttt ttaaggtaag tatagaaatg gcaatgagtt
     8821 gtggtgtaaa aaaagaaaaa atagtacttg acccaggtgt aggatttggc aaggattttg
     8881 aacaaaatat tgaagtattg agaagattaa atgaattaaa agatttaggg tatcctattt
     8941 tgcttgggac atccagaaaa tcagtaatag gaaaagtact tccagttgag cctaaaaaaa
     9001 gattagaagg tacaatagct actactgttc ttggtataag agatggcgta gatatagtta
     9061 gagtacatga tgtttatgaa aatttaatgg cagctagaat gactgatgca atatatagaa
     9121 agtaggtata atataatgga taaaatatta cttagtaatc taggattcta tggatatcat
     9181 ggtgttctta aagaagaaaa ttttctaggt cagaaattct ttgtcgacat ggaactttat
     9241 attgattcaa gagaagcagg tcttagtgac gatataaata aatctgtaag ctatgccgag
     9301 gtttacaatg ttgttaaaga tattactgaa aataaacaat ttaatctttt ggaggcttta
     9361 gcggaaaata ttgctgaaga agtcttgaat aaatttatac ttataaatgg agttatggtc
     9421 agagttagaa agcctgaggc tcctgtaaat ggtatttatg actattttgg tgttgagata
     9481 aggagaacaa gagatgaata aggcttattt gggaataggt actaacatgg gtgatagatt
     9541 tgataattta tcaagagcat gtgaactttt gaaaaatagt gattctatat ataaagtaaa
     9601 agaatcaagc ttatacgaaa caaaaccatg gggatataca gaacaagcag attttctaaa
     9661 tatgtgtgta gaaatagaaa cagagtttga accttatgag ttgttagaat attgtcaaga
     9721 aatagagaga gaactacaca gagagagaat agttcattgg ggaccaagaa ctatagatgt
     9781 agacgtacta ttttttaatg atgtagtttc cactgatgaa agactaacaa tacctcatcc
     9841 aaggattcaa gatagagctt tcgttttgat accacttatg gatttaaatg aagaacttat
     9901 aataaatgaa aaaacaataa aagaacattt aaacctccta tctgctgaag aaagagaaga
     9961 agtaaaggag cttgtaggtt atgaaagaaa acctatttaa taataattca aagatagaga
    10021 taaatacaaa taacaaaaag ataactgtaa aaaaagataa acttataata gctggaccat
    10081 gcgcaataga gagttatgag caacttttgg agactgctaa atttgtaaaa agtcaaggag
    10141 caaacatttt aagaggtgga gcatacaagc caagaacatc gccaaattca tttcaaggat
    10201 taaaaaaaga aggtcttgaa atattaaaag cagttaaaga cgaagttggt atggctgtta
    10261 ttacagaact tatggatgta agagatatgg acgaactgta tagtataagt gatataattc
    10321 aaattgggtc aagaaatatg caaaacttta ctcttttaag tgaagtggga aaacaaaata
    10381 aacctgttat gttaaaaaga ggaatagcat ccacaattac tgaatggata ggtgcttctg
    10441 aatatatagc aatagaagga aatagtaata ttataatgtg tgagagaggt ataagaacgt
    10501 ataatgatta tactagaaat acattagatt tagctgcggt acccattatt caaaaggaaa
    10561 caggtcttcc tgttgtggta gacccaagcc atgccactgg agttagatat ttggtaaaac
    10621 ctatgtcact ggcatctttt gcatgtggag cagatggaat aatggtagaa gtacatcctg
    10681 acccagaaaa tgctttgtca gatggtgctc aatctctttg ctttaatgaa tttgaagatt
    10741 taatgaaatc aattaataat tattaaaaga ggcaagagcc tcttttttat tcgtaatcgt
    10801 tgctaaggta aatttataag ttataatttg atagttagat tatttaatat aaataaagat
    10861 ttaatattga tagaattaat ataaaaaata aaaatttttt gattaataag aaggatatca
    10921 ataaaaaaag tcgaattgtg tatattgagt gattgacaaa tttagaatat agtgtagaat
    10981 agaaatgtta acacaaaaaa ttacaccaga aggtgataat atgaatgata taaaagacat
    11041 tattgaagaa ataaaagcta gatgcgatat agcaagtata atatctgaat atatgaatat
    11101 aaaacaatct ggagctaatt acaaaggatt atgtccattt catggcgaaa agacaccttc
    11161 tttttatata aatacatcaa aacagattta taagtgtttt ggttgtggtg aaggtggaga
    11221 cgtaataaat tttgtcatga aaatggaaaa tttggatttt atggatgctg taaaaatttt
    11281 agcaaataaa tgcggtatag aaattaacac taacatgaat gaagaaacta ggattaaaat
    11341 agaaaaatct aagaaatttc aagatattca tacggaagca gctagatttt atctttcaaa
    11401 cttattggga agcaaaaatc ttgggtatga atatttaaga ataagaggac tagatgataa
    11461 aattattaag aaatttggtt taggattttc tttggattca tggaattcac ttatgaatac
    11521 tctaatcagt aaaggatata aaaaacaaga cttattagag tgcggtctaa ttgcaaaaaa
    11581 tagagatgga actaattgct atgataaatt tagaaataga gttatgtttc ctatattcga
    11641 ttatagagga aatataatag gatttggggg aagagtatta gacgattctt tgccaaaata
    11701 cttaaattct ccagacacat taatttttaa taaaaaacag aacttatacg gattaaattt
    11761 tgctagaaaa aatttagaaa gtaaaactat agtcttagtt gaaggatata tggatttaat
    11821 ttcactttat cagtatggta taaaaaatgt agtagcaacc cttggaacag ccttgactga
    11881 acaacaaggc attttaataa agagatatgc ggacactgct attatatctt atgattctga
    11941 tgaagcagga ataaaagcga ctttaagagc tatagatata cttactaaat taggtatcaa
    12001 tgtaaaagtg ttagacttaa aagatgctaa agacccagat gaatttgtaa gaaaatacgg
    12061 acttagtgat tataaaaaag ctatggatgt ttctactcac tacataaaat ataaaataga
    12121 tcatcttaaa aaagaattta acattcaaaa agatgaagaa cgagtgaagt tcgcaaaaga
    12181 agcttctaaa attataaaac aattgacaag tcctgttgaa attgattttt atacaaagta
    12241 tttaagcaat caaatagata ttaatgtcga gtctataaag agagaagttt atggtaaaaa
    12301 ttacaataag ccatataata acaaaaatca aaagaaaata gaagaaaaag taattgaaaa
    12361 agtcgaagtc agacaagacg gaaaacaatt agttgaagaa actctaataa aaataatgtt
    12421 agaagacaaa aaaattagag aaatagcgtt acttaaagta gaagaaagtg attttttatt
    12481 gaaagaaagt aaagaaattt taaattacat gattaaaaat caagaattgg acaaaataac
    12541 tattgacaaa ttgaaaagtt taaacatatc cgaagaatac ttaaaagaat taaattcaat
    12601 ttctttgaat agtataaact tagagaacac aaaagaaata gaaggaataa taacaaatat
    12661 cagaaaaaac tcattagaag agcaaattaa tagcttgttg agagaacaac aagaattaga
    12721 aaataataat gatatgaaag aggtagatgg tagagttatg gaaattgctt taaaaatagt
    12781 tgagataaat aaaattttaa agagcttgta gagaaaggag gtttgagtgt ggaaaataaa
    12841 tcgaataaaa aagagttaaa aaaggttact gctaagacat taatagaaaa aggaaaaaag
    12901 caaggttctc tgacacttgc agagataatg gaagcttttt cggagactga acttgataag
    12961 gatcaagtag aaaatcttta tgagacttta ggaaatttgg gaatagaaat aacagaaaca
    13021 aaaaactata aagctgatat agatttttcg gttgctgatg acgatttaag tataggccac
    13081 ttagatgaag atgcagaggc aatttcacat gatgactctt ctgcaataga aatagaaact
    13141 gtggatttat ctttaccaaa agggataagt atagatgacc ctgtaagaat gtacttaaaa
    13201 gaaataggaa aaattcctct acttaaacca catgaagaag tagaatttgc tagaaggatg
    13261 cacgaaggtg atgagatagc aaaacaaagg ttagttgaag caaacttaag actagttgta
    13321 agtatagcaa aaagatatgt aggaagaggt atgcttttct tggatttaat acaagaggga
    13381 aatttaggtc ttataaaagc agtcgaaaaa tttgactata caaaaggata taagtttagt
    13441 acttatgcaa catggtggat aagacaagct ataacacgtg ctattgcaga ccaagctaga
    13501 actataagaa taccagttca tatggtagag actataaata agctaataag agtatcaaga
    13561 caactacttc aagaacttgg aagagatcca aaaccagaag aaattgcaaa agaaatggaa
    13621 atgacagaag ataaagtaag agaaataatg aaaatagctc aagaccctgt gtctttagaa
    13681 acaccaatag gagaagaaga agatagccat ttaggtgact ttattccaga tgatgatgct
    13741 ccagccccag cagaggcagc agcatattct ttattaaaag aacaaataga agatgtactt
    13801 ggttcattaa atgatagaga acaaaaagta ttaaagctta gatttggtct tgaggatggt
    13861 agagccagaa ctcttgagga agttggaaaa gagtttgatg taactagaga gagaataaga
    13921 cagatagaag caaaagcact aagaaaacta agacatccaa gtagatcaaa aaaacttaga
    13981 gattatttag actaaaaaga tacatcattg atgtttttgt gataaatgcc atgagataag
    14041 tgatatctca tggctatttt gtagtaaaga tttaatttta tttttgtagt tatttcagtc
    14101 taatatctat aacttggatt aaatcctata aataaaggga attgaaagga gaattatatt
    14161 tgaaattaac agatagatta ttgaaaatag catcattagt gtcagatggt aaaaaaatag
    14221 ctgatatagg aacggaccac ggatacatac cagtatattt gttaaaagag ggaaaagtac
    14281 catttgcagt gctagcggat gttaataaag gacctcttga caatgcacat aaagaagtca
    14341 tacagaacaa tcttttagat aaagttgatt tacgattggg ttctggtata gagatacttg
    14401 aaatagggga agtagaagaa gtgataattg ctggtatggg tggaatcttg ataagtgagc
    14461 ttctcgaagc taaaaaagaa gtagcacata atgtagaaaa attaatatta cagccaatgc
    14521 aagcacaaga agaacttaga tattaccttt taaacaacgg atatgaaatt ttagaagaag
    14581 ttttagtgag agaagatttt agaatatatg agataattgt tgccaaatat acaggtaaaa
    14641 atactataat agaagatgag atatattatg aggttggaat aaagctttta gaaaacaaag
    14701 attctctttt taatgatttt atagaaaaaa agataaagac atatagttct atagttaata
    14761 aattagaagg gaaaaatggg gaagctatag ataagaagag aaaggaaagc gaagttgcaa
    14821 taaaaaaact tgaaaattta ataaaataaa taggaggtat atatgctatt aaagagtcta
    14881 actagaaaaa ttgaaaaaaa gtatcctcta aacttagcag aggattggga taatgtaggt
    14941 cttatagttg gagattttga tatggatgta aaaaaggtat tggtatcttt agaagctaat
    15001 gaagatgtta ttgatgaagc tatatccaaa aatatagatt taattgtgac acatcatcct
    15061 tttatatttg ggaaaattaa taaaattaat agtggagatt taaaaggcag acttatccaa
    15121 aaacttatta aaaatgatat atcactttat tctatgcata cgaattttga tatagcattt
    15181 gatggtctaa atgactattt tatggaaatt atggaatttg gtaattcaaa ggttttagat
    15241 ataactaaaa gcgaaaattt gtataaatta gcagtatacg ttccacataa ctattcagat
    15301 gaacttagaa aggttttaag taattcagga gcaggtcata tagggaatta tagtgattgt
    15361 acgttttcaa cagaaggtga aggacaattt aagccattag aaggctctaa tccgtttctt
    15421 ggaagtgtaa atgatataga aactgtaaat gaagttaaaa ttgaaactgt agtaccacag
    15481 aagcttttag gtggggtaat aagttctatg ttagatgcac atccttatga agaagttgca
    15541 tatgatttgt acaagttaga aaataaaggt gagatatttg gactaggtag aataagtaaa
    15601 cttgataaaa gtatgacatt agaaagtctt tcaaggaaaa taaaagagaa attaaatatg
    15661 aaacacataa gagtagttgg aaaccttagt acagacataa cgaaagtagc cgttgtaaca
    15721 ggagctggtt cagaatttgt aaaaaaagct aagagacaag gtgcagaagt actaattacg
    15781 ggtgatgtaa agtatcatga ggcacaagat gcacttgata ttggaatgtg tatagtggat
    15841 tgtggtcatt ttgatacaga agatatattc aaaaatgtta tgaagagatt tttagatgaa
    15901 tttagtgaaa ttgaagtaat aaagagtaat gtatatttga atccatttag tactatttag
    15961 cagtgtttga taataaaaat actatataaa aaataaattt gagtttaatt agggggataa
    16021 tgatgaaagt agcagtaatt tttcatagtg tttgtgggag tacatattta ttagctagag
    16081 aatataaaga agcgcttgaa gaaatgaata tcgaggtaga tatatttaga gtaagtgacg
    16141 aagttgcaaa aacacttcct caatattatt tgataaattc caaagaatac aaagacgaat
    16201 ttgaaagtat aaatgtaatt aaatctggaa aagaaatatt agattatgat gctatattta
    16261 tgggttcacc aacatattat ggaaatgtat ctggaccaat gaagatgttt atggatagtt
    16321 tttctgatat ttgggtaggt gctcctttga gtggtaaaat atttggttgt tttgctacag
    16381 caggttcaca acatggagga ggagaattag ctttgcaagc tatgaatata tttgcccaac
    16441 atatgggaat gactcttttg tcagttccat gttcagtaag aggaggttat ccagcgtatg
    16501 gaatacttca tatagcagga gataattctg atataaggcc aaatgatgat gccaagatag
    16561 gtataagaga ttacctaaaa agacttaaca tataagaata tcatataaaa atacctaaaa
    16621 gcgactgtat atacttttag gtatttttac ttaattataa acgtgaagct ttaccagaaa
    16681 tatggtcaat ttctatttta acaacagttg ttaagttcat atccttctct atgtacttca
    16741 tgccttcttc aaagaatcct ttagaatatt tttttattat ttctacaaga gcatttttct
    16801 tttcttcatt ttctacagtg caagcttttc caaaaactat agcactagaa taagtagtac
    16861 taaatttatc tggaataaca ctttcattag caactactaa aaaagaaact ttgttgtttt
    16921 tcgatatatt atctaactta tgcccatttc ttgcacaatg aaagtaaata gaatcattaa
    16981 agtatacata atttacagca actccataag gataaccatt ttcagatata gtggaaaaag
    17041 taccaaattc accatttttt aatacttcaa cagtatcttc ttttgtcatt tctctttttt
    17101 tcaatctcat ttctttaaac ataatatcat cctttctaat tttgatgctt aaattaaatt
    17161 ttagaacatt tataaagatt atgctatgtt ttttacaaat tttaagaaaa aaatatagtt
    17221 taagataaaa ggtagtgaaa gagaagctat ttggaggttt atttatgata ttattgttag
    17281 agtagtacaa ataatactaa taaatttaat agctggtttc ttgttattta aggtagtaag
    17341 catgtctata agaaggggaa gttatcgagg gtagaagttg ttgtttataa cgatattata
    17401 aagaaaatat tattatataa aataataaat agtaaagagg tatcaagttg aatataatac
    17461 ttaatacctc ttcaaaatta agttataaaa gtgttgtggg aattgtccta tacatggtaa
    17521 ggagataata aagaatgtat ctttaaagtc tcaactaaac agcaaaattt atccaacaaa
    17581 catacaataa atgcttcttt gtatctaggt ggcttaacag taagtggcca catgtgtttt
    17641 aaaattatgt ccgtttcttt ttcatttatt tgaaaaaata aagttgcatt ttttaatgct
    17701 tctctagggt gagtaaatcc atgtaatcct tttctatcac ctttgtaatg ccaatcataa
    17761 agaaaaaagt cgtgtagtaa agctcctcta ataacacttt gaatatcaac acttaagtgc
    17821 aattttttac ataatagata actataatat gctactgaca aactatgttc aagacatgaa
    17881 acattgccat gatgaggaaa tgttctcata agttgaactt tttcagaaga aagtatctca
    17941 gaagcacatt cattaaaata tttgatttca gcttttgaca acattatgaa aatctccatt
    18001 cttagtaata tataaatact atacttcaac gataatatga aatatttctt atcctacaaa
    18061 atataatacc caataataca agtaagatac cttttatggt attttaaagt aagagagatt
    18121 ttattaggct aaacaatgaa aaaattaaaa gtaaagctat agcaatatat agtatttttc
    18181 caagaggatt gccaaaattt atagtccatc caataccaaa ccttttatta accattaatg
    18241 atgggtcatt tggattatta tacatgatgc ctgctatcca atacttctca tcttcttctg
    18301 gagaataaga tgagttgaat tttaaatttg gggaattaat atatgttgct attaaataaa
    18361 ttataactaa aagcgttgta atattcatag cagttaatag ttttgttttt attgataaaa
    18421 gagtagtaaa aaactgtgtt gtcattataa tcatcaatat taaaaacaaa taaccaattt
    18481 tatttaaata tttaatgttt tcaactctac tttcttcaat tctatttgta tctatcttaa
    18541 tccttgactt tatggatcca atggaagtta tatataataa aaccattaaa caaaagtcag
    18601 taccgattaa tttaaataca ttaagaaatg atttatctat aaaaacatca ggagacccat
    18661 ttatgttcca atgagtggca attgagtctg gcaattgatt ataattaaat gctataaaaa
    18721 tactcattcc caaagtaaat agtatcggta ttaaatagat tattttaaat ttttttatta
    18781 ttttattttt cttatttata aaatccatat ctactattaa ttttgaatct atatctatat
    18841 gacctaattt agcataaatt tctgatttaa gcttcttagc ttttttatgt gtatgaatat
    18901 atagtatact ttcatataat aaaaaaccta taataccaat aaaataggaa agttccaatt
    18961 tattaaatgt aaagaccaga ataaaaacta atataaagtc tataataaaa cctaaagata
    19021 atagtttttt aaattcttta tctaacttta taaagtaatt ataattttta tgctcttggt
    19081 ctatagaaac tccatagatg tgttttttac ctacaaaata gggtataaaa taaaatgtag
    19141 catacatcaa aattagaact ggaaatattg taaatagaca ttcaatctta ctcataatat
    19201 ttcacctcat acttgtcaaa taaacttgaa cttagtgaca aaaattcttc tttactaata
    19261 cctgataaat aagattttgc caccaataat tccaagtcaa gtttattttt ttgacgagtt
    19321 gtattatcta ttttcattgg tagttgagct acaatagcac cttttcttct atcaatattt
    19381 aagaatccct cttcttttaa ttcattgtag gatttgttca ctgtatgtaa atttacacca
    19441 atattttcag ccatacttct tacagatgga agtgattcat ttattttcaa ttctcctgaa
    19501 gctatagcct tagttatttc ttctttaatt tgtacataaa tagatttatc actattaaaa
    19561 tctaaattta aaaccataaa aaacctccat tatgctttat aaatgaaaac gattgttata
    19621 tacatagtat aacataaaaa ataataacaa acacaaaata taagtttata ttttgtaaaa
    19681 attgtaattt aaaaaataac ataattttta ttaaaataat acagatacta tttatagata
    19741 gaaataaaaa ttagaaagga gaaaaaatat gttaacatat ttaatgaaaa gacaaatgca
    19801 taaaaaagat gatggaatga gtaaaccaat gatgattaca ggagctgtaa ttacaggtgt
    19861 aggagcatat gctgtatata aaatggtaaa aagccataaa tctaattctc aaatggtcaa
    19921 tacaaattat tatggaaata gcgattatga tgattataaa tatgacgaat ttgattatga
    19981 ctgtgattgt agagacacaa aacaagatta tgaatatgaa gaattaaaaa aaaaagtaaa
    20041 agaatttaat tcaagaagaa taaattgtga aaattgtcca catgtttctc aagaagaaat
    20101 gatgcaatat gtaaatagtg ttaaagatgt aaaggaagag cttgatttac atgaagatgc
    20161 taaaaaagat aatatttaca aagaagatga atacaaaaaa tatgaagaag aataagtaat
    20221 attttgtata actgaacaaa tagtaatgtc aaattattta atatctagtt ttaatatgta
    20281 agttgtaact taacttgaaa gaatcagtat cattctttag atagttaagt aacaacttac
    20341 attttatttt tataaatttt aatagttctt tgtgactatt ttgtaatttt agaaatattt
    20401 tagtatccag ggtaattgtg tataataaaa cttgtataga tagatagtat gaaattaagc
    20461 agatagttta aaattaaaat gaaaagaaag gaaatagtta tgttaaagtt gttgatagtt
    20521 gaagatgata gtactatatc ttttggaata aagtatgctt tagaacaaga aggatttagt
    20581 atagatatat caaaggattt atctagtgga aaagaaatga tttcttctaa tgaatatagc
    20641 atgatactgt tggatgttac attgccagat ggaactggtt atgagctttg tcaatatata
    20701 agaggattct ctcaggttcc tataatattc ttgactgctt gtgatgaaga agtaaatatt
    20761 gtaatgggtc tcgatatagg tggagatgat tatataacaa aaccttttag aattagagaa
    20821 ttaatatcca gaataaaagc tgttttaaga agaaaaggta atacttcaga agaaaataag
    20881 aaaatactta aatttggaga tttaagtata tatacattag aagctagagt atataaaaat
    20941 gataaagaaa tatttctgac atcagttgaa tataaacttc ttttaattct tatacagaat
    21001 aaaaatactg ttttaagtag aactcaaata ttggaaaaac tttgggatgt aacttatgat
    21061 tttgtaaacg acaatactct aactgtttat ataaaaaggc ttagagaaaa aataggtgat
    21121 gatttatgtg aaccaatcta catagaaact gtaaggggga ttggttataa atggatagga
    21181 agtgaaaata gtgtttctat ataatccaga agtgaagaag tttttaagta agtatgttac
    21241 tttgatgttt attgttatca taatatctat tggatttagc ataatcaacg tgtccttaac
    21301 taaagagatg gttgtaagaa ataatcaggc tataatagga actttatcaa gcaagtatcc
    21361 aaacttagaa agtcagatag tagacataat aactcaaggg aagtctatgg agaacataga
    21421 ttatggtcag aaaatattaa ttaagtataa ttatgataaa tctattagga taaactctga
    21481 gcctattatt tctaaattag ttttagagac tatcaaaata aatataatat tagtatgtat
    21541 aatatttatt ttgatatttg tgttgattgt tagatatttt acgtcaattt acaatgattt
    21601 atcagatatg actaagtatg tttactatag ttcagagggt aagccatttg atatgaaaaa
    21661 caagaatcaa gaagggcaga taggtcttct aaaaactgaa ctcttaaaaa tgacgacaat
    21721 tcttaatgaa aaggttgaac ttttaaaaag agaaaagata tttttaaaca atacaatttc
    21781 cgatatatca catcaattaa aaactcctat gacttcttta ataatgttga atgatttatt
    21841 atataatgat gtaccatacg aagtaaaaat cgatttttta aataagataa aaaatcaatt
    21901 aaatagaatg gattggttga ttaaaagcat gcttaaatta tcaaaggtag aagcaaaagt
    21961 gataaatttt aaaaaagata aggtaaaatt tagtgaactc atacatagag caatgctatc
    22021 tatgaaaata cctatggaga ttaaaaatca aaaactgact atagagggaa atgataatgt
    22081 atcttatatt ggtgatattg attggtccgt ggaagcatta gtgaatataa tcaaaaattg
    22141 tgtagaacat actcctgaat ttggaaatat aattataacc tataaagaaa atccattatt
    22201 ttctgaacta ataataaaag atgagggtga aggtatagat aaaaaagata taccacatgt
    22261 atttaaacga ttctatagag gaagaagtag ttcaaaagaa gatagtgtag gaataggtct
    22321 tgcaatgtca aaatctataa tagaaagcca aaatggcgat atatatgtaa acagcgaaaa
    22381 aggtaagggt acagaatttc atataatatt tcataaaatg tatgatagtg actaatctgt
    22441 aatatttaat tcactttata gtaatagcca aaacctaata tatgaagtat agaatataaa
    22501 tattggaggt agttatggaa attttaagag tagaaaatct aactaaaagt tatggtaaaa
    22561 atgaaacaaa agttgatgct ataaaaaatg taagtttatc agttgaaaaa ggaacgttta
    22621 tagcaataac aggtccaagt ggaagtggta aaagtacctt attacattta ttaggaggag
    22681 ttgacagacc aactagtggt aaggtttata taaatgatgt tgatatatat aatttaaaaa
    22741 ctaaggattt ggcgatattt agaagaagaa atataggtct tatatatcaa ttttacaact
    22801 taattccagt cttaaatgtg aaggaaaaca tattgcttcc agcagagctt gacaatagag
    22861 atattgatag agaatatttt gatgatttaa tgaaaacatt aggtctaatt gatagagaaa
    22921 agcaccttcc aaaccaagta tcaggaggtc aacaacagag aacctctata ggacgtgcat
    22981 taattaatag accttctata atattagcag atgagccaac aggtaattta gatagtaaaa
    23041 attcaaagga aatattagaa ttgttaaaat tgtcagtaaa aaaatacaac cagacactaa
    23101 taatgataac tcatgacaaa aatatggcac ttcaagctga tagaattata agcatagaag
    23161 atggaatcat aaaaagtgat gaggtgatgt agatgaattt atatacatca ctaactatta
    23221 gatacttaaa acaaaataaa agaagaacca ttgtaaccat aatcggtata atactttcaa
    23281 cagctttaat ttgtggtata ggaaatatat ttgaaagttt tatggattat caaatgagag
    23341 aaactataaa gaatgatgga agtttccatg ttacatttta tgatgtaaat agaaaaaatg
    23401 tggaatatgt aacgaaatca gctgaaatag aaaaacatgc atttaccaaa caacttggat
    23461 attctaaatt agaaaatagt gaaaatgcta tattaagtat aaagcaatat gataaaaatg
    23521 caataaatgg atataaagtt tctataaaag aaggtagatt tccagctaag gaaggagaaa
    23581 ttgttttaag tgaaagtatt attaatctta tagatgataa gttaaaaata ggagataaga
    23641 taactttaaa agtaggggat atgtttgact caagcaagaa aaagattgat agtatgacat
    23701 ttcatgaagg tgattatata gcaaatgaaa aagatagaac ttttaaagtt gttggtataa
    23761 ttgaaaaacc tgggtttgaa cgttataatg gaattgcaac agctatagcc tactttaatc
    23821 ctatgaataa ttacaatgat acaattaatg tatcagtagc agtaaaaaat cctaaagatg
    23881 tgtataagat aagtaataaa ataagtaaaa atatatattc aaataaagat aaagaagcag
    23941 tttctgatat gataaaatac aatgaacatt tattgaggtt acaaggtgct agtaagtatt
    24001 caaatataaa tagctctata aagtctataa ttatagcagt aactatttta gtaatcgtat
    24061 gtacaattgc tacagtgtac aattccttta gtatttctat aaatgaacga aaaaaacagt
    24121 ttgggatatt aaattctata ggtgctacat ctagtcaaat taaaagatta gtttttatag
    24181 aaggtattat aattagttta attggtatac caataggact aatatctgga acagtagcta
    24241 tagacttatt gtttaaaatt ataaataagt attttactga atcggttgtt actaagatga
    24301 gtttgcagat agtgtataat ccaataataa ttattgttag tataataata gttttgctta
    24361 caatctttat atcaattcta ctacctgcaa tatcggcttc taatatttct ccccttaatg
    24421 tgattaaaaa tactggagag tataaagtgg gcaaaatcaa aagctcaaga cttattaaaa
    24481 tgatttttaa aactgaagga gtgcttgcat ataaaaatat taggagaaat agaaaaaaat
    24541 ttataataac tcttttttct ttaatggtaa gtataataat atttatatct tttagtggct
    24601 ttacattact tttactaaga ggtgaagaaa ttagaaattc tcaaagaaaa tatgatttgt
    24661 atttaactgc aaagaacaca gctaggtctg tagaggatac tataagtgaa ctagaagata
    24721 ttgatgggat aaaaaacttt gaattggcaa caggacaata tgtgtctata agagtaagtg
    24781 aaaataaaat aaataaatcc aaggaagatt taatcaggaa atattatcaa aaatataaga
    24841 ttggagatag ttatgaatat gatttcagta ataatgaatt aatttttcca ggagattttg
    24901 cagtaaaaaa caaaataaat aacttagtac agggctcttt taataaagag agagcaattg
    24961 aagaaaatgg tgttatatta gttagaaaaa gtgcaattga agaaaatggg aagaaaggtg
    25021 tagtagaact taccaactat aaagtaggag atacagtaaa ttgtgaatat cttgatgaaa
    25081 atgccttaag taaaaaagta aaggtcaaga ttcttgctat aactgacgaa gaacgtctag
    25141 gtcttggtta tcaaaatatg gggttacaat ttataactta tgatgaagtt gctaaaaatt
    25201 taaatttaaa attaagtaaa agccttatat ttattgattc aggaggaagc attaagacta
    25261 agaaggaaat agaagctttg gcaaataaaa ataattttaa cttttatgat gaaagtgcaa
    25321 gcaatgaaag tgaaaaacaa gatttaaagg ttattaaaat atttgtatat ggttttatag
    25381 tagtgatatc tttagtgagt gttacaaata ttctaaatac agtaagtaca agtataaatc
    25441 taagaaaaag agaacttgca gtaatacaat ctataggggt tactcctaaa ggatttagga
    25501 aaatgatata tttagaaagt tttatatatg gtatactgtc attgttgttt ggaataccaa
    25561 ttagtattgg tataattcta attatgaata aacttatttc aggtgtaatt gaattttctc
    25621 cgattatacc gtggacagcc atagttatct gtatagtaag tgtttttata ataactttta
    25681 tagcagggta tataccaatg agtaaattaa ataaagaaaa tataatagat aatataagaa
    25741 gagaaagtat atagatataa aaataattct taactatata aaatattatt aaaatcattt
    25801 aagtggtata aaatattatt atatcactta aataattttt tatttatata agaaatctaa
    25861 taataaatta ctagaggtat ttaactattt agaaagaatt ggtattatat aaagtaagag
    25921 aatatataat aagaaagaat atttgatttt attggaaaac aaaagaaaaa gaaggtgatt
    25981 actatttctc atataactgc gaagaatatg tataaaagtt tagaggaaag aataaacaag
    26041 tttccacaag gtgctccacc atctgataca ttatacaaaa tactaaatgt actatataca
    26101 gaacaagagg cgaagttagt tgctcagctt ccaataaagc ctttcagagt aaaaacagca
    26161 gctaaaattt ggagtgtaag tgaaagtgaa gcatatagag tattggataa acttgctagt
    26221 aaggctctga tactagatat agaagataat aaagggaaga aatatataat gcctcctcca
    26281 atggctggtt tttttgagtt tgccatgatg agaacgagac acgatataga ccaaaaactt
    26341 ttggcagagc tttactatca atatatgaat gtagaagaag attttatcaa agatttattt
    26401 tattcaacag aaacgaagct tggaagagta tacgttcaag aggaagtttt aactaatgat
    26461 aatgaggtta gtattttaga ttatgaaagg gctactcata taattgatga atcaactcat
    26521 ataggtataa gtatgtgtta ctgtagacat agaatgcaac atgttggaaa agcttgtgat
    26581 gctcctatgg atatatgtat gacgtttgac aatgtagcaa attctttaat aaataataaa
    26641 tttgcaagac gtgtggataa aatagaatgt aaagagctac ttcatcaagc atatgaacat
    26701 aacttagttc aatgtggaga aaatgttagg aaaggggtta cgtttatatg taattgctgt
    26761 ggatgttgct gtgaagcaat ggtagcagct aaaagatttg gaaatcttca tccagtacag
    26821 acaacaagtt ttataccaaa tataaaccat gaaaattgtg taaagtgtgg taaatgtata
    26881 actgcatgtc caatagatgc gataagcaag gttaaggaag atggtaaaga atatataaaa
    26941 attgatgagg atagatgttt aggttgtgga gtttgtgtta gaaattgcca taaaaatagc
    27001 attatgttat taaagagaga tgaaaaaata ataactccag caaattcagt acatagagca
    27061 gttcttatgg caattgaaaa agggcagtta caaaatttaa tttttgataa taatgctctg
    27121 gctagtcata gagctatggg agctatactg tcagctatat taaagcttga accagctaaa
    27181 aagattttag ccagtaaaca attaaagtca gtatatttag ataaattgtt aagtatgaat
    27241 gataaataga aactaactta aaaaaggcaa agttttagac ttatctatat cttaataaac
    27301 ttatcattta atatttaaat ttaattttta cacatataaa acttataact taaataaaaa
    27361 aaggctatat tcaattaata ttacttgaat acagcctttt ttagttatgt taagctattt
    27421 cttttatttg cttaaatgag ttttcatttc catttccacc aatttgtgtt acagatttaa
    27481 atctcatagt tttaaatagt tctttttgat tataatctag tgagtttcct actagcatta
    27541 caggagattt agtttttcca gccaaaactc caacagataa tccgtctatt aaatcatctt
    27601 gtttattcat tccattttta gcaacatata aattgtctat cttagaatcc ttgtaaaatt
    27661 cttttattac ttttgcatta gtttcatttc tagtgctacc tgatattctc tgtggatttt
    27721 gaagtttact ttctatattt ttagaaactg tatattctcc accgattaca taagatttat
    27781 caatcttttt gtttttaaac aagttattta tttcagttat attactattt tcattagtaa
    27841 gtattatagg catatcattt tgagctgata cagctccaat gcttactgca tcggctaatc
    27901 ctttttcgcc attaactact gaaatctttg atatattgct aatattgttc aattctttag
    27961 caatgttcat agaagtttga tatctatcac ttcctgaaat tctagataca gagatattgt
    28021 ttgattttat agtatctaaa gatttttcat ttatagaagc ttctccacca actacatata
    28081 catttttaac ttttaatctc tttaattcag ccaaagtttt ttcatttatt tgagagcttc
    28141 cagtcaataa tataggagcg tttttcttgt atgcaaatgg tgttgcagcc aaagcatctg
    28201 caattgctga atcgtttatt aaaactgcat tttcagcatt ttcccaacct gcttgactta
    28261 tcttaataga agtttcatat ctatcagttc ctgtaagtgc acttgcattt acttcatctt
    28321 gatatgtaac ctcaaatcta gcatttggac ttcctaagaa aacaattacg cctccttttg
    28381 gcaatctaac tctattgttc tttgtaacta aagaatagtt tacaggaaca cctttatcat
    28441 cataaacagc aaaagctgaa ttttgaggaa gattaacttc tattttttta tttgcgatat
    28501 cgtcaccaat cttataccat tttgcataac cattggattc tagttcacaa gtaaaagatt
    28561 tttctgctgg caaattagtt atagaatctt cactaacata cgttagtgtt ccaaaagata
    28621 aatactctgt accattttct ttatgaagtt taatgtcgga taaatcacga cctattacac
    28681 caggtatttc tataaaagca tttgagttat tctcatccat tattttagtg ttaactatat
    28741 aacctggagt ttcatcagaa agagcaagag ttgccttcac agaaccaaac atatagcttt
    28801 gagaagtata tttttcatct actagatagt aatctttacc atttcttttt ttccatgctt
    28861 cttttacact atttgatata ttgttgtcat ctatcttttg agctacatag tataaacttg
    28921 ctgtctgacc taatccaggg acatcatcat aagagctcat atttaaatat gtgatattgt
    28981 ttttttcttt tacaaatttt agacagctgt tacctttttc agatacaaat ctatcttgac
    29041 ctatgtatac gtatttatct tcaggtccat tttctatata aggagaagat actgttaaag
    29101 taccattatc attaacatct acttttatca tatttgaaga agcataaaga ccactatttt
    29161 cttttagaga gctaggcatt ttaacttgtt gtggtttact aaatgtttta tcaggtttta
    29221 tttctttaat tttacctttt tcttttaatg cagatagtaa gatttcttgt ccaattattt
    29281 cattaagttg actacttcca ccagaagaaa gaactgcaac tgccatattt tcatctggaa
    29341 gaactataag attactgtgg aataataaag agtctccacc ttttgtaaga gcttttaaat
    29401 tatactgatt aaatggataa gtgttgacac aatcccaccc taaaccatat ccaagtatag
    29461 agtcttctcc ttcaggccat agaccattta aatattcttt gttttccata gcttttactg
    29521 aagcaggaga aagaatacca tttgaatttt tcataaatgt ttgtgcaaat gtacatagat
    29581 tttctgcact tgaatataaa ccacctgcac ctatagcatt aaggttatct tgcggaactg
    29641 catcttccca ataaggcacg tatgcttttg ctagttttga actatcaaaa ctattttctg
    29701 tagtttttgt attttgtaag ttaagtggat tatttatgta tttatcaaga aaattagtaa
    29761 atgacatacc agatactctt tctactaaaa tttcagctaa agtaaatcca tcattacaat
    29821 aaacggaaaa agctcctggt ttagctttta atctttgttt ttgtaattct tttaagaaat
    29881 tatcatgacc ataagaatca ttgtctgcta ataaaatagt gtttttaaaa ctacttccca
    29941 tcaaccctga tgaatgattt aataacattc ttggagtgat ttccttgtat ctatcatcag
    30001 ccattttaaa ttcaggtata tactttacaa caggagtatc taaatttaat tttccatcat
    30061 caaccaactt cataacagct gttgtagtaa acattttact gatagatgcg atactataca
    30121 tattatcctt atttaagttt ttgttatcct gtttgctgta aactccacca ttaccagaaa
    30181 tctcaatttt gccattatca atcaaagcat attgagcact tacagcacca tactttgata
    30241 gcaaagtttg gattttctct ttagcaagag ctttagtttt tgtgtaagat tccgcgtttt
    30301 tactataatc atgtaattct atatcagaac tttgatattt ttcggaatta tcttttgtat
    30361 tatcagcaaa agtaatattt ggagcagcag taaataacat actcaatact aatgtactag
    30421 ataataattt agagagcctt tgcttaaaca tattacatta cctctttccc tttttaataa
    30481 tttataatta gcataacatt cttaattatt ttaatcaaca gattattatc aataaaaaaa
    30541 gcttttataa gtatagcttt ttat
//
LOCUS       DAMJYX010000036        30407 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJYX010000036 DAMJYX010000000
VERSION     DAMJYX010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.036"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            98..1162
                     /locus_tag="PC479_001791"
     CDS             98..1162
                     /locus_tag="PC479_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2804339.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1320..2006
                     /locus_tag="PC479_001792"
     CDS             1320..2006
                     /locus_tag="PC479_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804340.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            1996..3027
                     /locus_tag="PC479_001793"
     CDS             1996..3027
                     /locus_tag="PC479_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804341.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            3109..3774
                     /locus_tag="PC479_001794"
     CDS             3109..3774
                     /locus_tag="PC479_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804342.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3787..6204
                     /locus_tag="PC479_001795"
     CDS             3787..6204
                     /locus_tag="PC479_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804343.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6385..7176)
                     /locus_tag="PC479_001796"
     CDS             complement(6385..7176)
                     /locus_tag="PC479_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2804344.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(7209..7796)
                     /locus_tag="PC479_001797"
     CDS             complement(7209..7796)
                     /locus_tag="PC479_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2804345.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            complement(7973..9364)
                     /locus_tag="PC479_001798"
     CDS             complement(7973..9364)
                     /locus_tag="PC479_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2804346.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9548..10852)
                     /locus_tag="PC479_001799"
     CDS             complement(9548..10852)
                     /locus_tag="PC479_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2804347.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            complement(11211..12086)
                     /locus_tag="PC479_001800"
     CDS             complement(11211..12086)
                     /locus_tag="PC479_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2804348.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12290..12976)
                     /locus_tag="PC479_001801"
     CDS             complement(12290..12976)
                     /locus_tag="PC479_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2804349.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(13025..13558)
                     /locus_tag="PC479_001802"
     CDS             complement(13025..13558)
                     /locus_tag="PC479_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2804350.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13602..14234)
                     /locus_tag="PC479_001803"
     CDS             complement(13602..14234)
                     /locus_tag="PC479_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2804351.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14324..15403)
                     /locus_tag="PC479_001804"
     CDS             complement(14324..15403)
                     /locus_tag="PC479_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2804352.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSDVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(15536..16882)
                     /gene="pepV"
                     /locus_tag="PC479_001805"
     CDS             complement(15536..16882)
                     /gene="pepV"
                     /locus_tag="PC479_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2804353.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(16951..18231)
                     /locus_tag="PC479_001806"
     CDS             complement(16951..18231)
                     /locus_tag="PC479_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2804354.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18358..18618)
                     /gene="hfq"
                     /locus_tag="PC479_001807"
     CDS             complement(18358..18618)
                     /gene="hfq"
                     /locus_tag="PC479_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2804355.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(18732..19667)
                     /gene="miaA"
                     /locus_tag="PC479_001808"
     CDS             complement(18732..19667)
                     /gene="miaA"
                     /locus_tag="PC479_001808"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2804356.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(19705..21672)
                     /gene="mutL"
                     /locus_tag="PC479_001809"
     CDS             complement(19705..21672)
                     /gene="mutL"
                     /locus_tag="PC479_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2804357.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(21683..24526)
                     /gene="mutS"
                     /locus_tag="PC479_001810"
     CDS             complement(21683..24526)
                     /gene="mutS"
                     /locus_tag="PC479_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2804358.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(24686..25420)
                     /locus_tag="PC479_001811"
     CDS             complement(24686..25420)
                     /locus_tag="PC479_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804359.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(25389..26402)
                     /locus_tag="PC479_001812"
     CDS             complement(25389..26402)
                     /locus_tag="PC479_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2804360.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(26424..27194)
                     /locus_tag="PC479_001813"
     CDS             complement(26424..27194)
                     /locus_tag="PC479_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804361.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(27169..27459)
                     /locus_tag="PC479_001814"
     CDS             complement(27169..27459)
                     /locus_tag="PC479_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2804362.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(27590..27689)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      28309..28398
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28451..28627
                     /locus_tag="PC479_001815"
     CDS             28451..28627
                     /locus_tag="PC479_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804363.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(28742..29098)
                     /locus_tag="PC479_001816"
     CDS             complement(28742..29098)
                     /locus_tag="PC479_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804364.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            29565..30313
                     /locus_tag="PC479_001817"
                     /pseudo
     CDS             29565..30313
                     /locus_tag="PC479_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
BASE COUNT        10330 a         4810 c         3498 g        11769 t
ORIGIN      
        1 acgcttaaat tgaaaatata actttattct tatataatta aattatacaa caataaagtt
       61 atatttttta aatatatttt tactaggggg aatagaattg aaaagggttg atgagagaaa
      121 tactatgttt gcaagagcaa attacaaaaa agatagtact gcttataatg actattataa
      181 gaaaaatcct gacaaaaaag aaattgatga tagtataaga aatagaccaa atttatgtag
      241 tgaaggaaca atgacttaca atgagttaaa ctctccaatg gcaagtagtg cattcgattt
      301 tttatctgat atcaaatctt tatgcgaagg aaaagtttct gatactaaag tagatgtaga
      361 tgcaaagtca atgacaaaaa aaattaaggg tcttgcaaag cagtatggag cttctgtagt
      421 tggtataact aaattaaaag actatcatat ttatacaaat aggggtagac atgaagaaaa
      481 ttacggtgaa gaaattaatc taactcataa gtatgcaatt gtatttggat gtgagatgga
      541 taaagaaatg ataaatagag ctccaatgat atgtgaagtt atagaaacat caaagtgcta
      601 tgtggatgca tctatcgtcg gcatgatttt aagttactat ataagaaatt taggatatga
      661 tgctagaaat catatggatg ctaattattt agtaatgcct gtttttatag ctcgtgatgc
      721 tggtttagga gatataggaa gaaatgctat acttacaaat aaagattatg gctctagact
      781 tagattaggt gttgttacta cagatattcc tcttttagaa gatgaatatg ttgattttgg
      841 actagaagat ttttgtaagg tctgtaaaaa atgctctttt aactgccctt ctcattcgct
      901 ttctaatgat attaaaattg gtgatgatgg aaaatataat tgggtaattg aacatgagac
      961 atgctatatt aaatggagat atttaggtac agattgtggt atgtgtatat ctgcttgccc
     1021 atttagccaa aatctcgaaa ctataaagaa tactacttca tttaagggta ataatgagct
     1081 tattcaaaaa gctttagatg agtatacttc aaaatttggt aaaagaatat ttatacctgg
     1141 aaatccatct tggttgaaat aattttattt aataaagaat ctgtactaga atttatcata
     1201 ggacaggttc tttattaaat atataaattt ttaaatactt tttctcgttt ataacaattc
     1261 agcaatgttt ttagtctata attgacatta taaaatatta aactaaaagg agtaactgta
     1321 tggaaaaaat attagtagta gaagatgatt ctatattaaa taaaactcta tcatataacc
     1381 taattgaaga tggatatata attacttcaa aatttacagc taaatctgca ctaaagtcta
     1441 tttttgaatg tgaatttgat ttaataattt tggatattaa tttaccagat aaaagtgggt
     1501 ttgaattatg taatgaaata aaggaaaatt acaatatacc aataattttt ttaactgcta
     1561 atgatatgga atgtgatatg attaaaggct atgaacttgg agctttggat tatataacaa
     1621 aaccatttaa tatcaatata tttaaacaaa aaattaaagc ttttttaaat catcttacaa
     1681 taaaaacaaa acaagattac tatagggatg gatatcttga aattaacttt tctgagttat
     1741 ctgccaatat aaatggaaat caaattatat ttacaccatt agaatatcgt actttaaaac
     1801 tgcttactga aaatcctaaa agtattttaa caaggaaagt acttttagaa aaactctggg
     1861 atatagatgc taactttgta gatgaacata ctttaacatc tgttataagt aggattagaa
     1921 gcaaaattga aaaagacaat cttaagtata tcaaaacggt atatggtatg ggatatatgt
     1981 ggttaggtga aagaaatgaa ttttaaaaaa ttttctttta gttctattta tttaataatt
     2041 gctgttggta taatttttac agctatttta tttagtatct caatttatct aataactgat
     2101 gatttaaaac taattgctct attattaata tatacaatag tactttgtgg atttatagca
     2161 ttaataatat ttttacttca taaaaaaata gttatatttt gttctagaat atgtcacact
     2221 ttagatagta tgatgaatag tgaagataat attgaaattg aattagaaga agaaactatt
     2281 ttatcacgta tcaatcacag attaattaga atgtatgaag ttattcaaga aaatcgcaat
     2341 agtattgcta tagagaaagc tgatttacaa gaactagttt ctgatatatc tcatcaaatt
     2401 aaaactccta ttgcaaattt aaaaatgatt aatactactc tactaaatca aacatttaaa
     2461 acagaaattc aaaaggattt tttaatggat atggaaaacc aattagataa attagatttt
     2521 ttaatgcaat ctatggtaaa aacctccaga ctagagacag gtataatcac actatctaaa
     2581 aagaaaaatt caatttatga aacaattgct acagcactta gtggaatttt gtttgatgct
     2641 gaaaaaaaga atattgaagt tacagttgat tgtgacccaa atttatatat aattcatgac
     2701 aagaaatgga cttctgaagc tatttttaat atattagata atgctgtaaa atacacaagt
     2761 tctaatggta agatacgtgt agctacagaa tgttgggtta tgtatacaaa aatagatatt
     2821 attgataatg gaaaaggaat atcagaaagt cgccaagcag aaatctttaa aagattttat
     2881 cgtgaagaag atgtccatga catagaaggt attggaatag gtctctatct ttcaagaaaa
     2941 attatatctc ttcaaggtgg ctatattaaa gttacatcag atattggaaa agggtcgact
     3001 ttttctattt tcttacctaa taaataaatt tttaaatatt tcatttgtaa tgattctgca
     3061 acattttggt tttatactgt atttacaaga tgttaaggag gtattattat gagtatttta
     3121 caagtaatta atttaaagaa atattatgga caaagttcaa acattacaaa agctcttgat
     3181 ggtgtaactc tatctattga aaaaggcgaa tttatttcta ttgtaggtac atctggtagt
     3241 ggcaaatcca cattattaaa tatgatggga ggattagata atcctacatc tggaaaggtt
     3301 attataaagg gtaaggaatt atctactatg tctgatgaac agcttacaat ctttagacgc
     3361 agaaatatag gttttatatt tcaaaattat aatcttgttc ctttacttaa tgtatatgaa
     3421 aatattatct taccaatagg attggatgga gagaccatag ataaggattt tataaatgaa
     3481 attataaata tgctttctct tgaagaaaaa ttatatgata taccaagtaa tctttctggt
     3541 ggtcaacagc aacgtgttgc tatagctaga gcacttgtaa caaaaccagc tattatacta
     3601 gctgatgaac caacaggtaa ccttgatagt agaactagtt cagatgtact gagtttactt
     3661 aaagtgacaa gtaaaaaatt caaccaaaca attgtaatga ttacacacaa taatgaaatt
     3721 gcacaactaa ctgatagaat tattcgtatt gaagatggaa aaattgttaa ctaggaggtg
     3781 tagtatatgt ttagaaataa taatcgcaca tttataagaa aaatagcttt aaatgattta
     3841 aaaattaata aacttaaaac atatctttca ggtatcatta taatgatttc tacatgttta
     3901 ttattaacag tgaccttagt ttcatataat gcctctgtag atttagttaa tgcatctcca
     3961 tatcatgcta tatataaatc tgtagatgaa aaagcaaaaa atatactata taaagataaa
     4021 aactttagtg gcgtaggaac ttacaaatta attggaagta ataaaaagac agattgtatt
     4081 atgtcgattg tttatgcaga tgatacagca attaaactta tgaattttca accattgaaa
     4141 ggcaaactac ctacaaataa aaatgaaatt gctatttcag aaaaatattt acaggaattt
     4201 ggtttagata aagatattgg agatagcatt aaattaaatt attatgatga tattacgaat
     4261 aaagaagttc aatgtgattt tattattgtt ggatttctag aaaattatta tcaagataat
     4321 gccaagcaat actatacagt cgtttcaaat gattatttta agaatatagc ttctacacct
     4381 ttacaaaata aaagtaatac ttttgacgag tcaagacctg atactgtaga tgtattagtt
     4441 aaactatcag aagaaaatac agagaaagat tcagcttcaa ttaagacaca gttaaataaa
     4501 attggtttat ctttaggtat taagaaatat aatatatact taaatgataa ctatattgaa
     4561 tcaaatttaa ttgatggtga gcagattatt acagttttag tagttggaat agttgttctt
     4621 ttttctagcg tttttgttat atatagtatt ttttatattt cagttgttaa ttcagttcag
     4681 atgtatgcta agttgaaatc gttaggtatg acttcttttc agttaaaaaa aattatttct
     4741 ctacaaggaa atattttgtc aattattttt attccattag gggttatagc ttcttgtata
     4801 attgcttaca taattcaacc attagcatgg caaatgatag ctgatttatt tataatttta
     4861 attttatcac tggtaatgtt tttaactgtt agaatttctt tatttaaacc agctagaatt
     4921 atttctaaaa tatctgctat tgaagccatg caatatacag aaactaaatt tagaaaaaaa
     4981 gcaaaaaatt ttagttatat aaatataaaa aatctagcat taaagaatat cgagtcaaat
     5041 agaaagaaaa atttgattgc attaatatcg ctaagcatta gtggagtttt atttatttca
     5101 atcgctaatc ttgctaattc aatagatttc aaaaaacaac tatctcaaca atttgtctat
     5161 aacgaagatt atataattag cattaagagt gataacttat atgagcatat tacagaaatc
     5221 caacaaaata atccactaac agattctctt aagaatgaaa ttaaatctat tcatggagta
     5281 aaaaaattaa ttgaatccaa atcaattaaa agtaatatta tagaacctta catcaaagat
     5341 agagacgaaa aacaatttat tactctaatt aaaggtatta cgcctgaact ttcaaaatca
     5401 cttgaagaat atattgtcag tggaaaaata gactatacac atttagattc agatagttta
     5461 attataaata aatatagaac aaaatattat ggattaaatt taaaagttgg agatacagtt
     5521 acatttaata tctttagtgg aaatgatatt attaaaaaga aaatgaaaat tataggaatc
     5581 attgacaatt ccaatacagg aggtatgttt tttgcttcag acaaaacttt agaatctcta
     5641 acaccatata atacaaattt agatttttct attttaatag ataaaaagca ttctaaagaa
     5701 gttgaagaaa agttgttaag tataattcag aaaaattcaa atttaatgct ttattcatat
     5761 gaagatgatt atagaatgat tactagagca tttcaatata taattatagc ttcatacatt
     5821 tttgttggtt taatttcttg ctttggtata ctaaatatga ccaataccct aataaacagc
     5881 gttttaatta gaaaaaaaga gtttgcatta cttcaagccg ttggaatgac aagaaagcaa
     5941 ctgcgaaata tgctttatag agaaggtcta aacataagta tcaaagctat atgtacttca
     6001 agtatactag gttattttgg ttcaaatcta ctttgcactt ttataaaaga tgtaattaga
     6061 ttagacttta ttaattttaa attttctata tttactattt taattttttc attcgtatta
     6121 attggtatac aagttttagt gactgaatta cttgtaagaa atatagaaaa aaaatcactt
     6181 acagaacgct tacgttctca gtaagaatgt atataaaaaa tagccatttt ttatacaagt
     6241 tttaacataa ttttttaaaa gctaaaagga tagaaaattt tgactttaca taattttcta
     6301 tccctatact tgtttttatt ttatacagat acacttactt atatctccga gcaacatcac
     6361 cagagacata aacaagcata ttccttattt tctaaatatg tcaattgcat gacttgtaac
     6421 ccctactaaa tcttcacgtt cacaagtagc aaggtgatat tttaaatata atgcaaatgc
     6481 atcatcatcc atagagtcta ctgcttcacg catatacaaa gccaaaccat ctgatgcgac
     6541 ataatgcagt ctagttacat taaatgcaga catcaaatca tcaatgtttt ctttacgtac
     6601 aagctcaaac aaatcctttg gttcagactt agcagcaaat gtctgtggat caatcaaacc
     6661 tttttcaata tattcagata cattaatatt tcctctatgg aacccctcgt caataagaca
     6721 gccatcagat ataacatatg cagcaaaaac cacaccacca ggttttgtta cacgaattgc
     6781 ctcatgtaaa gcttgttgtt tatcttcttt gttataaaga tgatatagtg gtcctaacag
     6841 taacgtaata tcatatttat tatctgggaa aacagataaa tccatagcat taccctgagt
     6901 aatgcttata ttttcatttg attgagtatt tttatggaaa acctctatat tatgttcgac
     6961 taattccact gcgtcaacat catatccctg acgtgccagt gtgtgagaat aacgaccagt
     7021 tgcagcgcca atttcaagca cacaatcacc tgattttatg tacttatcaa tgtaatgcat
     7081 agtagtgaga aattccacca ttccatgttt taatgcaagt cgactatctt catcataagt
     7141 attataaaag tcaattaagt attgttttgt ttccataatt tcacctcttc tatattagtt
     7201 aataaacatc aaatagacaa attccaatct gatttttctt gtcttataaa agctgttaat
     7261 atatttttca acattttttc agcactatat ctatctaatg ttaatttttt tgttgctgtt
     7321 aagacagcta tgccatgagt ataaagaaat aaatctaaaa atattacttt tgcacattcc
     7381 aaatctattc caatgctttc cgaaaaaagt cttgcctttt tttcattgtc aatttcttta
     7441 taaaaatcat tcgcctcttt catcatcaaa tctaaatcat ttataaataa caacttaaat
     7501 aaatacgttt cttcttgagc aaattcaatg tatgaaagcg gaagcagtaa ataagggctt
     7561 acacttttag aattacgata attaacaaca tactgattat aatattcata tgcaaaagca
     7621 agaaattcat tttttaattc attcatgttt tcataacaag taaaaattgg tcgagtggaa
     7681 cactgtaatt tacttgcaat actcctcgca tttacagctt caaatcccac ttctcttgta
     7741 atatccaaga ctgtattcaa aatcatttct tttgttatct ttactttagg tggcatatat
     7801 gttcacctct ccattaaatt tttataaaac acatgtgtta tataactata gtaacatatc
     7861 aagaaattat gtcaagaaaa agctttgcat ttttttgcaa ttcctaaaac ttataaataa
     7921 aacatgagtt gcaattaatc tattaataga taacagcaac tcagattttc ctttaaatac
     7981 tcttcttttt gtctaatatt tcatatacac aatcttctat tgaattaata tctttttctt
     8041 ttcttttttt agaatcaatt aaaatgaaat acagtataaa acctacagca agtccccatg
     8101 ttccgccttt agcagcaatt acacctccca taagtccaat caatcccttc tcaacacctg
     8161 tattacatag ctccattgat agctttgtac ataaatatcc ttgaactaaa agtgttactg
     8221 acaatgctaa tggtataaga ggtgtagcaa tctgcgcaat tggataaaaa cagataaaaa
     8281 ttgctgaaac ccatactaat gtacccatgc cactaaaaac agactgcatg gcttcctttc
     8341 caaccttgta cctttgagca acacttcctg taagtgtcgc agctaatgga ccgcacattg
     8401 gtatatatgg agaaaagatt gccataaaaa tatttcttat cccacttata acattagacc
     8461 tatttgaatt gaaatctata tattcatctt ttctattttc actttcagaa agtactaagt
     8521 tttcacctgt tataaaatca ccaaaggcaa ttatgtatat cataaaagcc attggtatcc
     8581 cttgtataaa taacattaca cttggaaaac ctattccaaa aggagatact gtatttatca
     8641 gatttttaaa atctactatg taaactaaat tgtcaaaact aaatctagga atttctattt
     8701 ctttactaaa tatacctact attaaactca ctaaaatagc tgatactaca ccatattctc
     8761 ctaaatgaaa taaaaattta ttcttaactt ttagagtatc aaatctttca gaaaacatta
     8821 caaaacaagc aaccaaaatt cctactgtta tagatatagg ataagaatca aatctccctt
     8881 taggattaaa ctctgatatt atagtcgata taccgacacc cattagtatt ccacctttaa
     8941 tagaatttgg tatcttacta actaactttc cagctacacc agttataccc attaaaataa
     9001 atattattcc taaatctatt tgtaaagctg ttaatgcttg tattctttca atgcccatgc
     9061 tgaactttaa caaatatgca gttgtaagag ttatagaagc tgtaatccag ccaggtacaa
     9121 ctgggtctcc cataaaaaca tgcaaacagt ataatattga gttaatgata gccatactcc
     9181 atgccacttc atagggtaat cccaaaattt gtgtactaag gccaattgtg cctaaacttg
     9241 tcatacttga tattatacct tgaatagctt ctggtttctc aaatctgtaa tgtataaatg
     9301 gaattcttat cttaaacatt ccaaatttta tatgaggttg ttcccttcta gcgagtcttt
     9361 ccataacttc actccacctt tcaataactc ttataagttt acaactttat aattgtttat
     9421 aaattatcaa tgtcattaaa attagtatat aacatgcaaa ttatataaac aataaattaa
     9481 atctataaaa ttgatagatt ttgtgcatat atagaaagac cgcttcattt ttagttactt
     9541 aaatcactta atagtagtta tttaaatcac ttgataaagg ttttttataa aattcaatag
     9601 ttctattcca tgattcaaac tgtccttttg aatttccttc tacagttcca cctgaagaaa
     9661 aaatccctcc actcatactc atacataaag tctcctttag aggcataata tatggaattg
     9721 gtaacggatg acccatatta ttgtatgtta ataattctac gttatatgga taattatttt
     9781 cttcaaagat ttctaatgcc tttaaacaag cttcataact attccatatg ttatcttctt
     9841 ttccagcaat cataagtata tctgctttgg aattttctat ttttattcta gcctcttttc
     9901 tattctctgc tcttttaaga cttttcttat aagtactatt aaatccaaat ggaatacctt
     9961 ttgatatatt ttttttctgt tcttcaaaga atatatcatt gtctactttg atatatggaa
    10021 atggctctcc tttatatgac catgatgaag tatcatttcc actcatcaat ccattaagag
    10081 cttggaaaca ataaatgtga ggttgtgata ctacaacctt tttaatttgt tcatatcttg
    10141 aagcaagtaa aaaggccaat tctccaccct ttgatgtccc atgtacaaat atttctttag
    10201 tactggtaat ttcattttca ctaatccact caaatacttt ttcaaaatat tctaatggaa
    10261 cctcttctaa tttatctggt aacccttcta aagcaaagta tgcaactgtt agtacattga
    10321 atcctctaga tgcaagtgga gctgctaaaa ggtctagagc gtctaaattt ccatcagaac
    10381 cacctagcat taaaattgtt ttattattag aattttgttt ataaaataat tttcctataa
    10441 actcatcaga tatatatttt gtggtaatat cttctgattt aaatattctc tttaattcaa
    10501 cttcttttct ttcaatagaa gtttcaaata tcatattcat aattattggt ttatcaatag
    10561 atatattttc tgcaaaattt ttaccttcag ttttagattt ttccagcgaa taaattaacc
    10621 ccatacaatt atcagcctta taagtacctt ctataggttc aactaaatct aaatctactt
    10681 ctcctttttc atttgaacaa aacactccat aagatgaaaa ttcttctccc ttgcaccatg
    10741 gaaattccat tttcacactt attttaagtt tagtatttgg aattaagtcc gaaaccataa
    10801 tcttaagtct ttcatccaca aatgattcta cattgtatac atttactttc ataatctatt
    10861 tctccttata ataattattt atgttttata taaaacagct ttatctaata caacataaat
    10921 aaatttttaa attaagacac catattgact atttcatttt atcaacatat tctatttttg
    10981 tcaacatatt gtctttttta gcaatttaaa attatgtagt ctatatgaaa tccagttgat
    11041 ttttggctaa ttaaagatag gaatgctatt aggtttagaa tatatatatt caaaataaaa
    11101 aaccactcat tttaagaatg gtttttaata taaactattt gttattaata aggatatttt
    11161 tttatttaaa tttatataat cgaacatcta atatttaatg tttaataaaa ttatgcattt
    11221 gcaaaagtat atatggcatt aactcttgct acatataaat tctcatcact acatccaata
    11281 tatctagcat atttttcaat cttttctttt gcattttcaa catcattttc atcgctctcc
    11341 attaactcat ttatataaat atccattaat gacgctttat attctcttat atcatagcct
    11401 aaaaattcag tcaatctctc aatttcctta ctaaaatctc catcttcttt taccaaaaat
    11461 tcgtatacat cttccctata tggatttaaa gttattattt gttttactac tttgcattcg
    11521 tcagccttcc caataaaacc atctttataa ttattaaata aggcattaga tttttttata
    11581 gattcttcat caatataaga atgtaccgca gttatacgat tttctataag ggcatctaca
    11641 acagcataat gaattttaaa acaatcattg tataaataat tgctcatttt ctttattgtc
    11701 gattctattt ctaagaagct cgcgtggcca ttttttttat ttttatttgg tataataaaa
    11761 gttttattca agttattaaa aagtctctta aaatctaaat atttttcaca ataaacactt
    11821 ttaaaggtat tgatatctat agttataatt ccaaaactta ctatagtctc aattcctttc
    11881 ttaaaagact cttctatata tttttctcct aaacttaaac ctctatcaga tatttgcttt
    11941 agattttgaa aactatttaa acaatctctc tcaaagtttt tagatatttc caaagcatat
    12001 aatttaaaga ttttccttat cttattataa ttattttttg tatcatcaaa tctgataata
    12061 gctccattta catcaaaagt aatcattgag ttttaacctc cccattagtt tcatcaataa
    12121 aataaattta atataaactt tataatcata atattaaact tatttatact ataattatta
    12181 atttgatgat aaaagctatt ttccttctaa ataaaaaatt tgtctaaagt ttctaaaaaa
    12241 tttagacaaa tcttaggtta ttactcttta aattattttt aaattatttt taaattattc
    12301 caaatacgcc aaataatatt ggtataaata tagctggtaa caaattggct acttttatat
    12361 tcgttaattt aagtacattt agaccaagtc ctataattaa taaacttcca gctgctgtca
    12421 tttctgtgac tacaggatca cttaaaaatc catttaaaaa tgatgctccc acacatataa
    12481 taccttcata caaaaataca gatattgcag aaaacattac acctatacct aaagaagcag
    12541 taaatataat tgaagctatt ccatctataa cagattttac aaataaagta tcatgacttc
    12601 ctgtaagacc actttctaat gaaccaacta ctgccatggc tcctatacag aataataaac
    12661 tagatgatat aaaaccctct gctatagaat cttttttatt tcctttagaa aatttacttt
    12721 gtaaaaaagc acccaactta tttacccatt tgtcaatatc tattaattct cctatcagag
    12781 cacctacagc tacggatatt attgtcacta atgtgttttt tccttctagt gctcctgata
    12841 taccaatgta caatacacat aaggcaattc cattcattat agtatcgctt actctttgag
    12901 gtaatcctcc ttttattata agacctacta tacatccacc tgcaattgct agactatttg
    12961 ctattactcc aaacattttt tctcccctcg attagctaat tgaaatttat cctattatta
    13021 aaacctaaac atctatactt tgtattggat attttaaata ttccaaaaat tcttcaaaac
    13081 cttttctaga tatatttaca gttttagtat ttatgttagg atgaaaattt aagtaatctt
    13141 ctttaattac atctttatct atgtaaagct caacttttct ttctgtatca tttattaatg
    13201 aaaaaggatt tacacttcca ggaagaagtt taagtacatt gtatagtctt tcaggtgaag
    13261 caaacgataa tctagaactt cctatcttat ctttaagtga gtttaaatca acttgttttt
    13321 ctcctttaac tacaataaga taatacttat ttcctttggc atttcttaaa aataaatttt
    13381 tacattgaca cccctttgtt attccatcta aaatgactaa ttgctctgct gtatatacag
    13441 cttcatgctc tactacatca tattcaacat ttaatttatc caaaatatca tatattttag
    13501 tttcattcat gctagattca ttttcatctt gttctttttt attatttaaa tcactcatac
    13561 ttttctcctt aaacacttca tcataatatc attgtgaggt attattctac tatagctatt
    13621 gctcttactg gagctccatc agcatcatta tacttaaatg gtgctgctat aaatagaaat
    13681 ttttctggaa cattttctaa atttgtaaga ttttctatga ttatttttcc tttttcaaat
    13741 aaaatatgat gcacttcaaa tactgatgtg tcatatctat ccactgatag catatctatt
    13801 cctatgcctt ttatattagt attagctata tagtttgcag cttcttttgt taatgtagga
    13861 taacctacat agtactgctt tttatcccaa aattttgacc aacctgattt aaatattata
    13921 aaatcactat tttttatttc atcttcatat ttttttagat attctaattc tatattctca
    13981 tttccctttt caagttccaa tacataagct ttcccaacaa aattttctat atccaactta
    14041 tctagggtct cacctttagt atacatatgt cttggtgaat ccatatgagt tccattgtga
    14101 gaaaatacac taattaatgt ttcttgataa ccattttctt caataatagc tactttcttg
    14161 atatctggtc tattaggctc tgcatataca ggcatgtcat tatgtgtcac atgagttaaa
    14221 tcaaaaacct tcatctttat atacccccta tgtttttatt ttctattata tacctaaatt
    14281 cattttattt ctatcagatt ttattccatt atataccata ctactatata ttttttataa
    14341 acctcaaggg atttaccgaa aaatcttgga aagaattatc agtaaatata acatcttcac
    14401 ccaaaaaacg tttatacctt ccttcttctt tctttaaagt aattctctgt ttttgtgaat
    14461 gattattaaa aaccattatc ttaagaggag gtttttttct attagtctgc actcttttta
    14521 acatgtattt tatgtgcata tcagcttcag gaaatgaata tccacaaaat attaaaaggt
    14581 cagtatctct taaagttttt tctgtttcat tccaaacatc acttaaaaat atgtttgaca
    14641 tattcttaaa atatgttggt ggcactataa taggttctgt taactcacca cactctaaac
    14701 attttgtctc tgaactattt tcaataagct tcatgacacc accttcatgt ggtgtaagag
    14761 ttacactatt acaaatgggg caataaagcc aattcaatga cccatgtatc ttatataatt
    14821 tgattaatgg ttcttttggt tttctccaat ttaactcctc tttaaaattt gtaaactcca
    14881 caccataatc aagcataatt ggcaaatcat tcttatctct taatccagta actgtattgt
    14941 ctatatgtat atcataattt gcactaataa aagtagtatc aagaagcaaa tcattttcca
    15001 aaagattttc aactaaaagt ttatgatatt tattatctgt tctaggtgta ttatgaattg
    15061 cttcagccat tagcagtatt aaatattgtc ttaaaaaacg tatactatca cttctgttat
    15121 ttagattttc tagtccaaaa tttctaaatg cttcttttct ttgttctgct aagtctaaaa
    15181 gacctaaaac ttcttcaaag gtaggaaaat ttgcattatc tatattatct tttactatat
    15241 caatattgaa catctgttta aagaaaatat acaattctct attcattttg gtattataat
    15301 ttgtgggatt tagtttttta aaatattcgc taaaaagttc attttgaata ggtagattct
    15361 ctgctgctga agctcctgct cctaaaaata ttgctatttt catatagttc ctcctaatat
    15421 aaatattact cctattttta tatccattaa atatccatat catttatttt atgatacaac
    15481 aataaagctg tcttttacta gaaaaaatcc tattacaaga cagccttata ttatattaaa
    15541 tctttaataa ttcgtaaata gaaagagcat atatttgagt agcttttata atatcctcta
    15601 ctattaagta ttcattagct tggtgttctg tttctggttt tcctggaaaa atagctccaa
    15661 atgcaacaca attatctaaa gctcttgcgt atgttgctcc tccagatgaa agtggtgttc
    15721 catcaagacc agtttcttct tcatatactt ttctaagtgt cttaacaaga agagtatctt
    15781 ctggaacata gattgaatcc aagaaatcat attcttcata atttaaattg tatttatctg
    15841 tcattttctt taactctttt acaaaatcat cctttttgta tgttactgga atccttacat
    15901 ctattcctgc aaattctttt tcgttatcaa tagttactct acctatattt actgttagtt
    15961 tacctgatac atcatccttg cagttaggaa ttatattgtt tccattagca tcttcaccaa
    16021 taacttctgc taaaaactta attatattag aatctatacc tatattatta agagctatac
    16081 aaagtctagc cactgcattt ataccagtat cacttgctgc tgaatggaca ctctttccaa
    16141 ttatacagat tttattacct tcaacagtat actcaaaatt taatttatta agttcttttt
    16201 ttaatttatc agagtatttt ccaatataga ttgctttacc tggaactgca tttaaagcct
    16261 ttccaactga caactctata tcacttttgc tatcacaagt caaatgaact tgtaaaagac
    16321 ctttctctgc atttgttatt gggaatctag aatctggagt aaatccatag tttggtattt
    16381 cttcattatt ttctttatat ttatttatac atctccaaag attttcttca tcagccccaa
    16441 aaatgaatct tatcctctta ttaaagtcta catttaaatc cataagagct ttcactgcat
    16501 aaattgctga tattgtaggt cctttatcat cttgtgttcc tcttccatac aatttaccat
    16561 cttctaaaac agcttcaaat ggagggtaat tccagctttc taaatcacct tctggaacca
    16621 catcaacgtg accaagtata cctatcattt tttcaccttg tcctatttct gcatatccat
    16681 aatatccatc tttgtatact gtcttaaatc ctaaagattt acataaatct aatatacctc
    16741 ttaaagcttt atctacattt tcaccaaatg ggcaattttc agttgcttca gatataacac
    16801 taggtatttt tacactttct tgtattgaag aaatcatttc atcttgtaga gaatttactt
    16861 tttctttaat ttgctctttc atattatcac ccttcaattt tcatcataat atactaataa
    16921 atattttgac cttttaaaaa aaaaatatgc ttattttttc agttttttta taaattcaat
    16981 cgcttttaaa gtccccattt ttgaatggtc aaatgtaagt ccaccttgga aataaacatt
    17041 ataaggaggt cttattggag catctgcact taattctata gaagaacctt gtataaatgc
    17101 tcctgctgcc attatgactt cactttcgta tccaggcatg tcccaaggaa caggttttac
    17161 ataagaatcg actggtgcag cttcttgtat accttcacaa aaacttatta cttcatcagc
    17221 attattaagt ctaatacatt gaataatatc acttcttaag tcatcatatt ttggtaatac
    17281 atcatatcct aatttttcaa aagctcttga gcaaaagata gctcccatta cagcctgaga
    17341 tactatataa ggcgccaaga aaaaaccctg aagtacattt ctagttgttc caaaagtaag
    17401 accacattct tttcctattc caggagaagt cattctataa gatataagct caatcaaatc
    17461 ttttctacca gctatatatc cacctgtcaa tgcaagccca ccaccaggat tttttattaa
    17521 agagccagcc ataacatctg ctccaacatc agtaggctct ttggtatcta aaaactcacc
    17581 ataacaatta tcaaccatta ctatagcttc tggtttaaca gactttatta cttctatagc
    17641 ttcttttata tcactaattg aaagtgattt tctccaagaa tatccttttg acctttgtat
    17701 cataacgagt ttagttctat catttatttt gtttttaatt ccttctaaat ctaagtttcc
    17761 atcctctaaa aaatctacat catcatatgt aacaccatac tctttcaatg aacctttttc
    17821 ttctcttatg cctataactc cctctaaagt atcatatggt ctaccagtaa cagataaaat
    17881 ttcatctcct ggtctaacta taccttgtat acataaagta agtgcatgtg taccatttac
    17941 aattattggt cttacaagag catcttctgt attaaatact ttagaataaa tttcctctat
    18001 tttttcacga ccaatatcat tatatccata tcctgtagtc cagttaaagt gcatatcact
    18061 tagtttagat tcttgcatag cttttaaaac tttatattga ttatattctc ttatttcttt
    18121 tatttcttca aacttatctt ttatttcttc cattatttct tgcgatagtt taaaagtatc
    18181 atcatctatt ccatagtagt cttttaataa ttcttttgtc tcattaagca ttttatcacc
    18241 tgtctcttta ttattttatt taaacatgta aaggagacta ttttactaaa acaaataaat
    18301 tatagtttat aagttagtat aagtctcctc tcaatcatct taaattaaat taattatcta
    18361 tctgttgtta ttattattgt tgttttggtt ttgatttgtt agattaatat actttccagg
    18421 ttgtatagtt gatacagcat gtttataaat catgttttgt tgtctattat ccccttctat
    18481 taatattata taactatcaa acccctttac aagcccttta acttgcactc cattaactaa
    18541 atatatagta acaggtatcc tttctttcct tgcattattt aaaaacaaat cttgtaaatt
    18601 taaaactgta tttttcatta gatttgtacc ctcccaagat taaattgaac ttctaaaata
    18661 ttcctacctt atattctata taagttatga caaattttca atattaagtt gtatacttaa
    18721 aaatttatat attattttat agaatcttta atatataaga ttatttcatt ttttaattcg
    18781 tctatatttt catattggtc taaatcaaac cattttgctg ttttatatct tttaaaccat
    18841 gttatctgac gttttgcata ccttctagaa tctcttttaa ttatttcaat agcttcttcg
    18901 tatgtatatt ctccatccag atactttaat atctctttat aacctatacc ttgcattgat
    18961 atcatatctt ttttaaatcc catacttaaa agttttttta cctcttcaac caatccattt
    19021 ttaagcataa tatcaactct catatttatt ctttgataca aatgttctct atctctattt
    19081 agaactatta ttattggttg atattcttca ttaaatttta aatcacttga aaaatcattc
    19141 attttcttac cacttagaca aacttcaaga gctcttatga cccttttagt attgttttta
    19201 tggattctat tagcagattc ttcatcaagt tcttttaact tattatgcat gtaatctatt
    19261 cccttttcag caagttgttt ttgaagttct tctcttaatt cattattggc atcagattgt
    19321 gcaaaatcca tattatatat taaagagttt aaataaagtc ctgtgcctcc agttactaaa
    19381 acacattttt cctttttatt tatttcatgt atatattttt ttgctctttg ttgaaactct
    19441 gaaactgaaa atggatagtc aggcttaact tcatctataa ggtaatgctt cactccttgc
    19501 atctcttcct ctgtaacttt tgcacttcca atatccatat attcatatat ttgcatagaa
    19561 tcagctgaaa ttatctctgc attcatatct tttgctaatt ttattgacaa atctgttttt
    19621 ccaacagcag ttggacctgt aagtattata agtggtattt ttttcataac attcctttcc
    19681 atatatttta aatatccaaa tattttacat aattctttta aacatttttt ctatttctgt
    19741 cttagatatt tcgaccatta taggtcttcc atgaggacat gtaaatggat tctcacactt
    19801 ttctagctgt tccaataaac tttttatttc aatatcgtat attttatcat ttgcttttat
    19861 agcagaccta catgccatag atgcaaatct ctctcctttt aaatcataat tacttgtaat
    19921 ttcttctata ttatcaatta tttgaagtat aaatttctcg gtttcaggta ctccaaatat
    19981 agtaggaaca catcttacca ttatatgatt attgccaaaa atttctagct caaaaccaaa
    20041 tttcataaat aactccaagt tattttcaat ttgaagcata tctacatttg atacttcaat
    20101 aaccactggg tctaaaagta tttgcatatt tatatcttgt ctatagaact tctccatata
    20161 tctttcatat agtattcttt catgtgcagc atgttggtcc aacaaataca tagaatcgtc
    20221 tttgctcaaa attatataag tattaaatac aactccaatt acagaatatc cgtataaaga
    20281 aaacttgcgt tttgaactac acaagtattc ttcttcactg tccttaatac tatctcctaa
    20341 gtaataattt ccttcatttt taattccatc aacttgaaac tcttcttgta tattatcatt
    20401 tgcactatct tcacttagat aattaatgtt ttttacatca tcttctacac ttgcatttag
    20461 gacttcactt actgattgaa attcatttat tggtttttca cttgataaag taacaacttc
    20521 aatgacttca tccttacctt tagtaatatt actattttta ggagtagatt ctaataaatc
    20581 attatttctt aatttataat ctgtactttt ttctctactg ttaatagcga ttctaggttg
    20641 tgttttttta tcactataag tagcatattt tccaataaga tttgagtgta ttaatttaac
    20701 ctttaaaaaa tctctaagtt ctatatacac ttcttgttct ttttcaaatt taatttcaag
    20761 tttattgggg tgtatattta catctataca tgatgggtct acttctatat ttaagaagca
    20821 cactgcatgt ttacctattg gaattataga cttataactt tctgttattg catctatgat
    20881 tattttactt tttacaaatc ttttatttat atagatatgt tgtaagtttt tatttgaacg
    20941 gtatatattg ttattaccta tatatccatt cattttaaag tgattgcact taaactctac
    21001 atctattata ttttccgtta tttcttttcc atatatactt ctaatagtgt ttacaagctt
    21061 tccatcccct ggagtattta gcatctgttt attgttattt gtatatttaa attgaatgtt
    21121 tggattacct attgcaagtt tatttatcaa atcacttata tttatagttt ctgcatgagt
    21181 agattttaaa aatttctgtc ttgctggtgt attaaagaaa atatctttta taatgatggt
    21241 agttccatta gtagaaccta ttggttcttt cgatattatt tttccacctt caacatatat
    21301 tttagttcct attatttcat ctttagtttt agttgtcatt tctaattttg atacagctga
    21361 aatagatgcc aatgcttctc ctctaaaacc aagagagtat aaatcataca aatcatcaat
    21421 ttttttaatt ttactagtag catgtctcaa aaatgatttt tcaacttcac ttgaaggtat
    21481 tcctattcca ttatctgtaa tcttaattag actttttcct ccatcaatta tatctatgga
    21541 aattttattt gcacctgcat caatggagtt ttcaataagt tcttttacta cagaagaagg
    21601 tctttctaca acttcacctg ctgctatctt atttatagtc aaatcatcta ggatgttaat
    21661 tatatttttc atttttacac ctctaagatt ttatttcttt tgcttttctt tgaagattat
    21721 aaagagagtt tatagcatct aatggagtca tatttaaaat atccagattt aaaatttctt
    21781 ctgatagtat atctctattt acactatcaa aagagatttg agttaaggct acttctttta
    21841 cagaatccac ttgattaatt ttctctttat catcattttg ctttattaaa gcaacttcat
    21901 catttaatgc tttaaattta gaatttaatt tatcataatc cttttttata tgtttgtagt
    21961 cttttactat aagttcatgt tctattttta gagattcata tttattctta aaattacttt
    22021 gatttacctt aactaattct tcgtccaaat tactattaat tatattattg ttttctttat
    22081 ctccatttat tgcaacactg ttgtaaacat gatttttttc caagtcttta agtatatatt
    22141 ttgctctttc tatgacttcg tctggtaatt tagctaattt agcaacatat attccataac
    22201 tcttgtctgc tccctgagga attatttttc ttaaaaatat tattccttcc ccatcttctt
    22261 tgactgctat tgaataattt ttaacctctt taaactcttc ttctaaatca gtaagttcat
    22321 gataatgtgt tgcaaataat gtcttacatc taatattttt ttgaatatat tccactattg
    22381 accatgctaa acttatacca tcataggtac tagttccacg acctatttca tctaatataa
    22441 ctaaacttct ctctgttgca ttttttaata taagggatac ttcgttcatc tctaccataa
    22501 aagtagattg accttgagat aaatcgtcac tagctccaac tcttgtaaat attctatcta
    22561 atattggaat atcagcagac tctgctggta caaatgagcc tatatgtgcc ataagagcta
    22621 ttatagcagt ttgtctcata tatgttgatt tacctgacat attaggacca gttattatgt
    22681 taataatgtt ttctccacga tttaaataag tatcatttgg aacgaaattt tcctctccta
    22741 ctatattttc gacaacagga tgtctaccat ttcttatatc aagtttatta ttttcattta
    22801 ttgctggttt tacatagtta tttatatgtg caactgttgc aagggacaca aaaacatcta
    22861 tattagctat agtcttagcc actttttgta ttctatctat gtttttatat attgtatctc
    22921 ttatctctac aaatatctcg tattctaaag atttaatctt ttcctctgca tgtagtatct
    22981 tttcttctat ttcctttaat tctggagtta tatatctttc tgcattacta agtgtttgct
    23041 ttcttatata tgtctcatct agctttgcct gtttaaagtt agcttttgtt atttctatat
    23101 aatatccaaa aactttatta aagcctattt ttaatgattt aactcctgtt ttttctcttt
    23161 ccctgttttc tatttccttt acaagaaagg ctccattctt agaaatctct cttagttcct
    23221 taagttcatc actaaaatct gatttaatta tgtttccatc ttttatggtt atcgtaggtt
    23281 cttctagaat agcttcatct attaaattgt atatatcatc taatttatcc atttctgaaa
    23341 catactcttt taggattttt gcatttgata aatttatagt atcttttaaa ttaggtaact
    23401 tttctatcga attttttaag tgaattaact cttttggtgt tactctctca aatgctatct
    23461 ttccacatat tctttctata tcgtatatat ttttaagtat atcatttagg tcttctctta
    23521 atataaagtc atcttttatt tcttctatta catctaatct attttcaatt ttagatttat
    23581 ttatcaaagg ttcttctaca tattttctta aaagtctacc tcccattgct gtagaagttt
    23641 tatcaagtac gtgtaataat gaaccttttt tcttattccc tcttatagtt tgagtaagtt
    23701 ctaaattagt tctagtaaac atatctagaa ccatatattc tgatgaattg tatatattta
    23761 tattattaat gttagatgtt atttgttttt gagtattgta tatataattc agaagaattg
    23821 ataagcttga ctttattaga cctttatcat caaactttag tttttgtaga taaacatctg
    23881 gaaaatattc ttttaatata tttatatcca aatagttatc actaaaactt tcatttatgt
    23941 atatattact tacagtagct atatctctaa gattttctat aaagtctaaa tcatttataa
    24001 ttatttcagt tggatggatt tttgcaattt cttcaattac cttatcttca tttaaacatg
    24061 ttgcattagt ttctcctgta cttatatcaa cataagttag acctatatta gtaccatcct
    24121 tatacaaaga taataaataa ttattctttt tattttcaag taaatttcca tctaaaactg
    24181 taccaggagt gatgactctt ataacctctc ttctaacaat tccttttgct gtagatgggt
    24241 cttccatttg ttcacctatt gctactttat atccattttc aactaatttt gatatatatg
    24301 aattggctga gtggaaagga acaccacaca taggagcacg ttcttctaaa ccacaagctt
    24361 ttcctgtcaa tgctatttca agagcttttg aagctactaa tgcatcttca aaaaacattt
    24421 cataaaaatc tccaagtcta aaaaacagta tacagtcttt atatctattt tttacttcaa
    24481 gatactgttt catcatagga gttaatttat tcatatctat ttgcattatc aaacctcctt
    24541 cttttctaat taaatattat atcatagtag atgattatta ttaacacaat atataaaatt
    24601 ttgacatata actaaaaaca atccaaaatg tatactcaca ttttggattg ctaagtttaa
    24661 tgcttattaa ataaaaatat tttttttaca ataaagatat tatttctttt ttaagtttaa
    24721 tgatattttc aaggttatct tcatttaact ttctactatc tataacaaat tctttcttta
    24781 ctattgatgg tttttttgaa ataacataaa ttctgtttga tagcattata gcttcgtcaa
    24841 tatcatgagt tataagaagt attgttgaat ttactttttt cttaacatct aaaagccatt
    24901 tttgcatgga tgttttagtt aatgaatcaa gtgccccaaa aggctcatct aaaagcatta
    24961 tgtcattaga atttataaaa gttctcaaga agttagctct ctgtctcatt cctccagaaa
    25021 gttctgaagg atatttatct tcatatccgc ttaatccaaa cacctcaaaa taaggtttta
    25081 ccattgacct agctttactc tttttttctt tttttaaaat tagtggtagt gacacattat
    25141 ctattatagt tttatatggc aagagtaaat ctttttgata catataacta tacttaccat
    25201 ttatattcac tttgccacta tcataatctg taagtttagt tataatatta aaaatagtac
    25261 tttttccaca tccacttggc cctaatatag aaacaaactc accttcataa acatcaatac
    25321 ttatatcttt taaaacttca atatctttat aagtttttga tacattttct atagatactt
    25381 ttactttttt attgtggtaa aaattcattt gtgtaagcat cacttgcctt catatcctta
    25441 tctattaatt tatattcttt taagaatgat gtatagttgt tccatacact atctttcatt
    25501 tctccccatc gtggtgcatc atctttgtac ttgcttgcta aatattcttg actctttaaa
    25561 gctagttttt catctacttc aggtgcatgt tttactaaaa tttttgctga ttcttctgga
    25621 ttttttattg catattcata accttttgtt gtagctttta agaatttttt agcaagctct
    25681 ggattatctt taagcacagt ttcattagaa attataagtg gtgtataata atccaatctc
    25741 tcatctaaat cttttactgg tataaagttt aagtcatatc caattaaatc agctttaact
    25801 gcatcccaac cttcaaatat ccatgcaaaa tctacagttt tcatagctgc aaagaaatca
    25861 tcttgaccag tattaactat ctttaactta ttaaaatcag ctccattttt cttcataact
    25921 gctttaaaaa cagcttcttc tgatggagag ccccaaccac cataagtttt tccttcaaaa
    25981 tctttagctg ttgttatatt cttttctttt ggtgatgcaa acccagaagt attatgttgt
    26041 ataacagttg ctacagcttt tattggaagt ggatcttcac tagtctttgc ataagtaact
    26101 tgttcttgat agcttatacc aaagtcagct tttcctgttg ctactaaagt ttctgctcca
    26161 ctttcaggtg gttgtactat ttcaacatcc aaaccttctt ctttatagta acccttatca
    26221 agtgctacaa ataatccagt atgatttgta tttggtgtcc aatcaagcac catagttact
    26281 tttttagaag aagtatcttt atcattgttt ttgtctttat tgttatttga agaacatcca
    26341 gttattgttg ccaatgtaaa aacacttact aaacctaata ttcctaactt tttaaaattc
    26401 ataattgcct ccctcaaata cctttaatct attaatctat gtcttataac aacttttttt
    26461 acagcctgta caattcctac aaacattaaa ctaaatataa ctactaatat agtagatgca
    26521 aatactttat caagtgcata tgcacttttt gacctaagca tataaacacc aagtcctttc
    26581 gttccaccta gccattctgc aacagttgct gccattacag catatgttgc agatatttta
    26641 agtccagaaa aaaatttatc cattgccata ggaaatttaa ggtgaatgaa tgtattaact
    26701 ttatttgagt ccatagtctt aaataaattt aaataatctt tatcaatatt ctcaatgcca
    26761 tctacaaaac ttattaatat aggaaaaaaa caagtaagca ttaccatcaa tacttttggc
    26821 attgttccaa atccaaacca tatcataaag agtggtgcta taattattgt aggtatcatc
    26881 tgtgtaacca acataatagg ataaagacat ttttttatta ttgatacaaa atccatcaat
    26941 attccaatta ccaatgatat tataattgcg actataaaac caagtattgc ttctcctata
    27001 gtaaccacag catgcatgga taaattttga taatctttaa aaaacacatt aattatatct
    27061 actggtgtgg gtagtatata ctgaggaact tctaaaactc ctaccataga ttgccaaaga
    27121 ataagtataa acaaaaatgt tgctattgga tatatactat ctttgacttt atctatgatt
    27181 tctaagtttt tcatctatag tcagacctcc agctttttca tctactctag ctactgttat
    27241 gactctttca catccaagat taaaagggat tttatgtacc ttttttaata attcaaaaac
    27301 ttcatcaaaa tcaccttcaa cagtagtgct cattgctcct atttcacatt taagaccact
    27361 tttttcaatt tcagcaatac aagcatctac tactttatac atctcttctt cacctttgta
    27421 aggtcttagt ggcattactg ctatgtctgc gattaccatt taaatcactc ctttaaaaat
    27481 aattaatacc tactaggtat attttgtttt ttattaaaaa attttattac taaaaagata
    27541 ttatatttaa ataaaagaaa agtcttatcc aaaataggat aagacttatt tttatcttaa
    27601 ctggtctccc tacactggca ttatccaaac aggttcaaag ggtcgagata aaatctcctc
    27661 tcagcacatt agctccccga aatatattat atcttttcta cttgattata ctacacttat
    27721 catattttaa caaatttttt atttatatta gatattttat ccgttcaaaa atatctaata
    27781 taaattgtac attattacac caaataaatt atcttaaaat ggtattttat caacatcttc
    27841 caaaactaga ttaactgaat tttctagttt ttattttgct atttcaagca tatttggatg
    27901 tatatcaatt ccatacaaat attgtgctaa atttctgttt tcactcatct aatttgcaat
    27961 cgttacagat tacagattag aaacagtttt tagactctac ttatctttat ctacaaaaaa
    28021 tctttcaata aattcattaa taatattcag taatctaaaa ataaacctct atctggattt
    28081 ttaagataca aaccttttag gaagtatctt ttgtgataat ataaaaatta ctcgtcatta
    28141 tcatcaaaat cctcagcaac caacatttta tataagaatc aaactctata atataattat
    28201 tttatccaat ttatttttga gaaataaaat atttttttaa tttttaaatt ttttaatatg
    28261 caccttgaaa aaagaaacgt tttcctatat tatggtaata gtagaataaa actaaatagg
    28321 caaaactaga gaaatttagt gacgcaaagc tatagggact aagacttatg aaatgagtta
    28381 tgtcagccag ttgccaaaga gatattttct ttttgttttt atgaatgttt tttaggggga
    28441 tatatgatat atgtttatgg ataaaatgga tagatgtaca catatactaa ctgcatatat
    28501 atgcagttca cgcgattact gcaattttat agatacacaa ttaaatgatt ttatactaga
    28561 atatggagag aatgtggtag aatcttgttt gcatcaagtg atggtattgg taagcagata
    28621 taattaaaga aattttattt tctagaaaac cctttttagt tttatctacg ttttataatt
    28681 cattaaagaa ctaattaaaa taagcttcat tatatatttt tcatgaagta aacagttaag
    28741 tttatctcat atactgttta ccccaattac ataattctaa taatatagat tccaatgatt
    28801 taccacgttt agtagacaaa tattcaactt ttggaggaat agtcggataa gaaatcctct
    28861 taataagtcc atctctttca agatacttta attcttgtga cagtatctta tgacttatat
    28921 gaacaacttc tttttttaat tgactgtaac gtattggctc ttgttttaaa atatgccata
    28981 aaataattgc cctccattta cttcctatca cagtcatggt aaaaccaatt gaacaattaa
    29041 aatctttttg tctttcttca atatttggaa attcttcaaa attattatct atatccatct
    29101 caatcctctc tttcaaaata gtaagtatct aactaaaaag tgcattcttg tttagcgtac
    29161 taattcgtaa tataatcttg attaaaaaat agctatgcac actcgtgact ttagtcatgg
    29221 gaggttcagt gtagcataga gccttacata atactacttt tttagatgta tatgtttaaa
    29281 ttttaatata gacatatgtt attttattaa ttttatttaa acagataata ataaataata
    29341 atttatctca agttttctat gtagataaac taatctaatg tttagaaagg attatataat
    29401 atgaataaaa aatttcgtct ttatcaagtt gactcattac aaaagaaaaa tttacaggta
    29461 atccagctgg tgtaattaca aatgctgatg gcttatctga atcacaaatg caaaaaatag
    29521 caagagaatt aaataattca gaaactgctt ttattttcaa taacttggct aaaaatcatc
    29581 aagaaaatta tgatgtacat atcagatttt ttacaccaac taatgaggtt cctatatgtg
    29641 gtcatgctac tattgctgcc cattatgtta gagcaataga aaaccaattc aataacaaaa
    29701 cagtatttca aaaaactggt gctggaatat taccagttga tattataaaa aaagataatg
    29761 attatgaaat aattatgaca caaggaaaaa ttgaagtatc tccaccatta caacaaacct
    29821 atcaaaaaag attacttaat gcatttggta tttaaaggaa gatattcgta cagattgtcc
    29881 tattgccata tcatctacag gacattcaaa agttatgatt ggaataaagg aattatcaac
    29941 attaaatagt ttatctccaa atttatatga attatcgaaa ataagtcaag aaataaaatg
    30001 taatggatat tatattttta cattacataa taaagaaact ctacaagttc atggaagaat
    30061 gtttgctcct gcaattggta tttcagaaga ccctgttaca ggaaatgcta atggaccctt
    30121 aggtgcgtac cttgtacatt ataaagtaat acctacaccc aacacagagt ttatatttga
    30181 agcagttcaa ggagaagcaa taaaacgcaa aggcaagatg ttagtaaaag taattatttt
    30241 agataataag ccagttgaag taaaaataat tggaaaagct gtaattgcat tctcaacaaa
    30301 catagaaatc taattataga ttccttctaa gtaaattttt gaacttttta atactaattt
    30361 aataacaaac taatttatat acaatcaaat taattttttt attattt
//
LOCUS       DAMJYX010000037        30268 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJYX010000037 DAMJYX010000000
VERSION     DAMJYX010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.037"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            31..1143
                     /locus_tag="PC479_001818"
     CDS             31..1143
                     /locus_tag="PC479_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2804365.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1397..3421
                     /locus_tag="PC479_001819"
     CDS             1397..3421
                     /locus_tag="PC479_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2804366.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3641..4327
                     /locus_tag="PC479_001820"
     CDS             3641..4327
                     /locus_tag="PC479_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2804367.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4422..5477
                     /locus_tag="PC479_001821"
     CDS             4422..5477
                     /locus_tag="PC479_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2804368.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5592..7148
                     /gene="murJ"
                     /locus_tag="PC479_001822"
     CDS             5592..7148
                     /gene="murJ"
                     /locus_tag="PC479_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2804369.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7194..8900
                     /locus_tag="PC479_001823"
     CDS             7194..8900
                     /locus_tag="PC479_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2804370.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="PC479_001824"
     CDS             8922..9995
                     /locus_tag="PC479_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2804371.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="PC479_001825"
     CDS             10007..10729
                     /locus_tag="PC479_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2804372.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="PC479_001826"
     CDS             10745..11902
                     /locus_tag="PC479_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2804373.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="PC479_001827"
     CDS             11905..12744
                     /locus_tag="PC479_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2804374.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="PC479_001828"
     CDS             12759..13934
                     /locus_tag="PC479_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2804375.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="PC479_001829"
     CDS             13963..14712
                     /locus_tag="PC479_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2804376.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="PC479_001830"
     CDS             14734..15642
                     /locus_tag="PC479_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2804377.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="PC479_001831"
     CDS             15690..16442
                     /locus_tag="PC479_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2804378.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16396..17763
                     /locus_tag="PC479_001832"
     CDS             16396..17763
                     /locus_tag="PC479_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2804379.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17769..18860
                     /locus_tag="PC479_001833"
     CDS             17769..18860
                     /locus_tag="PC479_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2804380.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="PC479_001834"
     CDS             18944..20857
                     /locus_tag="PC479_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2804381.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="PC479_001835"
     CDS             21056..21763
                     /locus_tag="PC479_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2804382.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="PC479_001836"
     CDS             22065..24176
                     /locus_tag="PC479_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2804383.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="PC479_001837"
     CDS             complement(24374..25339)
                     /locus_tag="PC479_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2804384.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="PC479_001838"
     CDS             complement(25624..26580)
                     /locus_tag="PC479_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2804385.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="PC479_001839"
     CDS             26948..27736
                     /locus_tag="PC479_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2804386.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="PC479_001840"
     CDS             complement(27859..28329)
                     /locus_tag="PC479_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2804387.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="PC479_001841"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="PC479_001841"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2804388.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="PC479_001842"
     CDS             29155..30060
                     /locus_tag="PC479_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2804389.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12000 a         3298 c         5373 g         9597 t
ORIGIN      
        1 atttcaaata atgctgtaga ttctaaactt atgaatagta ggattaaatc aatagatata
       61 ataagagggc tatcaatagc cctcatgata gtctgcaata atcctgggac ttggatgaga
      121 atgtatcctc aattaagaca tgctgtatgg catggtgtaa cacttgcaga ttttgcattt
      181 cctttttttg ttatatcttt aggtgttaca attccaatat caataaatag taaactaaaa
      241 aataataaat ccacactgag tataatactt agtatattca agagaagtat cttattgata
      301 ttatttggat ttttcttaaa ttacctagga aatcctgatt tagatactgt aagaatactt
      361 ggagtacttc aacgtatggg attagtctat tttgtgacta gtttagtata tttactacta
      421 aaaaaattaa atgttggaag tactgctaca ataattactt ttctatgtat atctacattt
      481 ataatagttg ggtactacat attagcaaaa ccttatggat ttgagctaga aggttctctt
      541 gcacagttag tggatttaca tttcttcaag ggacatttgt acaagccgga atttgaacca
      601 gatggttttt taactagtat agtagcaatt tcttcaggta tgttgggatg cactatggga
      661 tgtgtacttt taaaagaaaa cataggagaa tataaaaaat tctttaaaat actggttatg
      721 agcataatcc tgctaatagg tgcttttatc tttaatcagt attttccatt taataaaaga
      781 ttatggtcta gctcatttgt acttttaatg gctggcagtt atggtatatt actgtcaata
      841 ttttatttta tatgcgacat caaaaataaa agtaaaatat ttaccccaat aatagcactt
      901 ggaagtagcc ctatttttac atatatgtgc cttgaaatat tgagtcatgt tttttggaat
      961 gttccaaaac ttacgaacaa agtcgattat ccaactacct tggtagagtg gactacatat
     1021 gaacttataa caccttgggc aggtacaact tgggattctc ttatattttc tttattgtat
     1081 gtcctattct gggttatagt aatgtctata atgtacaaga aaaaaatatt tattaaaatt
     1141 tagtgcaata tttttagatg ataaagtctt tgatttgttc aaatatacgg aaactatcaa
     1201 atataatcaa aatttctcct cgataaaatt taaaaaccat gccataaaag attacaaaaa
     1261 gtttacaatt tgtaattttt tgtgctataa tgaaaaaaga tttacataat attgaattta
     1321 tgtaatataa aacaagctta agatgtatta aaaaagattt aaagatttaa agaaccaaaa
     1381 aattaggggg gatatgatga aaaaaacaac aaaattattg gcaacaggaa tgttgtctgt
     1441 agcaatggtg gcaccaaatg tagctttagc ggctgaaaat actactgcaa atacagaatc
     1501 taattcagat attaatataa atttacaaag aaaaagtgta gtgttaggaa gtaaatctaa
     1561 tgcaagtgta aaatttaaag aaaaattaaa tgctgattca ataacattaa attttatgtg
     1621 ttatgatatg ccacttgaag ctactttaaa ttacaatgaa aagacagatt catatgaagg
     1681 tgtaataaac tataacaaag acccagaata cttaaatgta tgggaacttc aaagcataaa
     1741 aataaatggt aaagatgaac agaaagtttt aaacaaagaa gacttagagt ctatgggatt
     1801 aaatcttaag gattatgatg ttacacaaga gtttataata agtgatgcaa attcaacaaa
     1861 agcagtaaat gaatatatga gaaaaacttc tgcaccagta aaaaaacttg ctggagctac
     1921 tagatttgaa actgctgttg agataagcaa acaaggttgg aaagatgggt ctagtaaggt
     1981 tgtaatagtt aatggagaat tagcagcaga tggaattaca gctactccac ttgcatctac
     2041 atatgacgca ccaatattac ttgcaaataa agatgatata ccagaaagta caaaagcaga
     2101 gttaaaacgt ctaaatccaa gtgatgtaat aattataggt gatgatggtt cagtttcaca
     2161 aaaggcagtg tctcaaatta aatctgctgt aaatgtaaat gtgactcgta ttggtggagt
     2221 tgatagacat gaaacatctt tacttatagc aaaagaaata gataagtatc atgatgttaa
     2281 taaaatatac atagcaaatg gatatgctgg tgaatatgat gctcttaata tttcatcaaa
     2341 agctggtgaa gaccaacaac ctataatatt agctaataaa gattctgtac cacaaggtac
     2401 atataattgg ttatcctcac aaggtttaga agaagcttat tacataggtg gtagtcaaag
     2461 tttatcatca aaaattatag accaaatatc taaaatagca aaaaatggta cttcaaaaaa
     2521 tagagtttct ggagcagata gacatgaaac aaatgcaaat gtaattaaga ctttctatcc
     2581 agataaagag ttgagtgcaa tgttagttgc aaaatctgac ataatagttg attcaataac
     2641 agcaggacct ttagcagcta aattaaaagc tccaatactt ataactccaa aaacttatgt
     2701 ttctgcatac catagtacaa acttatcaga aaaaacagca gaaacagtat atcaaattgg
     2761 tgatggaatg aaagatagtg ttataaattc tatagcatct agtttatcaa aacacaatgc
     2821 accaacagaa ccagataatt caggttcagc agcaggaaaa actgtagtga tagacccagg
     2881 tcatgggggt tctgattcag gggcaacaag tggattaaat ggtggagcac aagaaaagaa
     2941 atatactctt aatacagcat tagcaactac tgaatattta cgttcaaaag gtataaatgt
     3001 tgttatgact agagatacag ataaaacaat ggctttagga gaaagaacag cattatcaaa
     3061 tactataaag ccagatttat ttacaagtat acattacaat gcgtctaatg gatctggtaa
     3121 tggtgtggaa atttattaca aagtaaaaga taaaaatggt ggaacaacta agactgcagc
     3181 atcaaatatc ttaaaaagaa tactagaaaa atttaatatg aaaaatagag gaatcaagac
     3241 aagaacactt gataatggaa aagattacct atatgtgtta agaaataata attatccagc
     3301 aatacttgtt gaatgtgcat ttattgacaa taagagcgat atggataagt taaatactgc
     3361 agaaaaagta aaaacaatgg gaactcaaat tggaatagga atagaagata cagtaaaata
     3421 aaatagaaaa tattaaagca aaacatatca tctcctgtta taatgtaaaa tataacaaga
     3481 gatgatatat ttttgtaaag aatcaaattg tttacaatga tgtttcttgt attgataatc
     3541 tgctcattat tgagtataat atagaaataa gtcaaaaata agatagaaat aagttgaaat
     3601 taagaattaa aaaaattttt attccaatat ggaggtaact atgtctggat atactaatga
     3661 tgagtgtgaa atacctaaaa ttaaaagcta tccaggggca gataaagaaa ttgcaagtga
     3721 gattgactat agcatagtaa aaggaacagt tttattcgac ttgtaccaaa gaattatgga
     3781 tttagtttta tctataatag gacttgtgat aggtttacct ttaatagcaa tatttggaat
     3841 tcttattaaa atagaggaca aaggaccaat aacttataag caagaaagac taggaaaatg
     3901 cgggagaaga ttctatatat ataaattgag atctatgaga acagacgcag aaaaatttgg
     3961 cgcacaatgg gctgaaaaag atgatcctag aataactaag gttggaaagt ttattaggaa
     4021 aactagaatt gatgagattc cacagctgtt taatatatta aagggggaca tgggcttaat
     4081 aggtcctaga ccagagagac cgaactttac tgttcaattt aatgaggaaa tacctggatt
     4141 tatcaacagg ctcgctataa aacctgggct tacaggttgg gcacaagtaa atggtggata
     4201 cgaaatcact cctgaagaaa aacttaaaga agatatttac tatataaaaa ataggagtat
     4261 attacttgac tttaagatac tctttaaaac tgttaaagta gtattgacag gagacggagc
     4321 tagataattt taaaatatta gattactaat caatgaaaaa gctctttaaa aagagctttt
     4381 ttattgtcaa aaaatacaaa aaagaaaagg aatgagaact tatgattaaa aaaatttcta
     4441 ctatattatc gttagttttg ttaatctcta tttctagtac gataggagta tttgcagatg
     4501 caaatcccaa aagagaattg atagaaggga gtataccaga gatttcgaca gaattaaata
     4561 aaagagcatt taaggattct aaagaagtaa ttcttgtaaa tgaagagtca attgtagata
     4621 gtataagtgc aacaccttta gcatatgcaa aaaatgctcc aatagttgta actaagagta
     4681 aaaatcttgg tagagtaaca agaaattatc taaaagaact aggtccagag aaggttacga
     4741 tagtaggtgg tttaaaggca gtatctaaag atgctgaacg taatatagaa aaaatgggaa
     4801 tgaaagtaga aagaataaga gggaaagaca gatatgatac atctttaaag atagctagag
     4861 aaatgtatag aacagtagga tttgatgaag catttttact tagctcaaca acaggattag
     4921 agaacgccat atctgtatat tcttatgccg ctaaaagtgg aatgccaata atatgggcta
     4981 aagatgaagg gtttgaggaa caaattgatt tcttaaaagg caaaaatctt aaaaaaatat
     5041 atgcattagg tgattcaaaa gagtttattg cagagattga ttctaactta aaaaatatag
     5101 aaggaataaa gcaaataaat aagtcaagta caaatgttga tttaataaag aagttttatg
     5161 atgaaaaaga tataaagaaa atctatactg ctagattaga ctttggtagt agatctgatg
     5221 taaacgaata tatttctttg ggagtagtat cagcaaaaga aaatatgcca atactgattt
     5281 gtagtgataa cttaagtcgt gcacaagata aattcttgaa agatagcaat ataaatgatg
     5341 tagttgaagt tgggtataca gtaggtgatt atagtctatt taagagcata tttaatctaa
     5401 cattcttatc ttgtatagta ttaatactat tattactatt gattacattt agggcactta
     5461 gatatgagtc gaaatagttt ttgatttata aggaagcttt tcataggtta aatttactta
     5521 attagaataa tgtttataag cagttttaaa agttctactt ttagagttta aattagataa
     5581 ggagaaagaa gatgtcaaaa acagctaaag cagcattgtg gattatggca gccactatgt
     5641 tttcaaaagt attaggtttc cttagagaac ttgtacttgc aaatttctat gggacaggta
     5701 tgtatgcaga cgtatttgta ttaacattaa atatacctgg tttaatcata gcagtcatag
     5761 gctctgcagt tgctacaact tatataccta tgtactttga gacaaagaaa agactcggag
     5821 atgaaggtgc cttaaaattt actaataatg ttttaaatat atgctatata atggctatag
     5881 taatagctat tattggtctt ttatttacag agcaatttgt tacagtattt gcagcaggat
     5941 ttagaaacga ccctgctaag ttccaagcag caatattatt tactaaaata atgatttcag
     6001 gagttttatt cctgagtgga agtaagatat ttagttcgtt tttacaggtg aatgacagtt
     6061 ttgtaattcc tgggcttata ggaatcccat ataatattat aataatagca gcaatagcac
     6121 taagtgcagg taaaaatgta tggatactgc cagcaggagc actattggct atggcaagtc
     6181 agctattgtt tcaactgcca tttgcgttta agaagtccta caagtataag ccatatataa
     6241 atttaaagga tgagtccata aaagagttag tcaacctagt attgcctatg ttggtaggag
     6301 tagctgttgg acagttaaat atatttgttg accgattatt ggcaaccact ttgggtgatg
     6361 gaaagttgtc tgctcttaac tatgcaaata ggctaaatga gtttgttatg gcattatttg
     6421 ttacatctat aataacagtt atatatccta aactagcaaa aatgtctggt aaagataata
     6481 aagaaggatt tataagtact attgtaaaat cttcaaattg tattatattg gtggtgcttc
     6541 ctatatctat aggagctatt atattagcag agcctcttgt tagaatatta ttccaaaggg
     6601 gtaaatttga tgcgttatca acagacctta catctatagc gcttagatta tattcactag
     6661 gtttattggc ttgtggagta agagatgtct tatatagggc attttactct ctttcagata
     6721 caaaaacgcc aatgataaat ggaagtatag ctttaataat aaatatagtt cttaatttaa
     6781 tattgataag accattgggt catgcaggta ttgctatatc aactagtaca tcaaacataa
     6841 ttacagttat attattattt atatctctta aaaagaaaaa tggatatttt ggaggggata
     6901 aaattataaa gactggtcta aaaagtttgg tagcatctgg tgtgatggca gtagctactt
     6961 tattgatata taataatcta tatgcattta tgggttcagg aacaattaaa gaaattatat
     7021 cagttggagc tggagtatta ggtggagctt ctgtatatac tgtattgata gttttattta
     7081 aggtagaaga gatggattta gcttttgaat ttttaagaaa aggaaaacaa aaactactta
     7141 gaagatagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgaacaa acgaaaaatg
     7261 agctattaag tataaaagat gatgaaaaag aaatacaaga tagattttat aaaaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaaaact caaggagaag
     7441 aaggtaagca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagtac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa gccagtaaga aaagtcttaa atgaatgtgg ctttgaaaat gtaatggtag
     7981 ttaaggagca agaattgcca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa caatggcact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggc atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggt tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat actatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tactagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa gatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa tatcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacaa
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagagc
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aagataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatatgga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgac aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcagcat gttttttaat
    11101 ggtagctata ttattattta atctatttcc ttcattgata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actaccaatg ctcctaaaga cattaccgat
    11461 aatacttact ttatttagtg taattattgg tacagtattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggacagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag acaatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaataaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagacta tacaagattt gatgagaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataacta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attataagtt tgcaaaagtt atggtatcaa aacttgtaag aaatatagga
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagt tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt ggaaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatttt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagta gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta tagaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gattatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagtaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcaacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaagaagat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggttcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat gatgtaaaag tagaacttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctac atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaggaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa taggatatgg tggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaggttgctg ttttaggttt gacttttaag ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgctcgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaacta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagcgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcctataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa tccagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaaaata aaattggaag aataatccac aatgtgaaaa ttgtaggaac
    19561 aacttcagat ataaaagatg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaattg cttagaaaat ataaaaataa
    19981 attagattta aaaacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatt attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtacttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat ataaatgggg agcaactgga ccaaattctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggagtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca cgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggatgattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttga aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatga
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatatata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagcatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggtcagta cttattatgc aaagtttaaa attgtagaag atttgacgaa
    27361 agtagaagga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 tataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgcc ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatagat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaaatcctt tgcatcaaag atttttgatt cctttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggactaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatggttg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agacttgata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actaatcaac aacaaaatgt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttgcaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaattttaaa
    30241 aaataaattt agtataatgt aatgtagt
//
LOCUS       DAMJYX010000038        29853 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJYX010000038 DAMJYX010000000
VERSION     DAMJYX010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.038"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(729..1238)
                     /locus_tag="PC479_001843"
     CDS             complement(729..1238)
                     /locus_tag="PC479_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2804390.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(1449..2858)
                     /locus_tag="PC479_001844"
     CDS             complement(1449..2858)
                     /locus_tag="PC479_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2804391.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(2862..3992)
                     /locus_tag="PC479_001845"
     CDS             complement(2862..3992)
                     /locus_tag="PC479_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2804392.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(4004..4807)
                     /locus_tag="PC479_001846"
     CDS             complement(4004..4807)
                     /locus_tag="PC479_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804393.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(5189..6070)
                     /locus_tag="PC479_001847"
     CDS             complement(5189..6070)
                     /locus_tag="PC479_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2804394.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            6526..7500
                     /locus_tag="PC479_001848"
     CDS             6526..7500
                     /locus_tag="PC479_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804395.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(7616..8677)
                     /locus_tag="PC479_001849"
     CDS             complement(7616..8677)
                     /locus_tag="PC479_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2804396.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(9289..10140)
                     /locus_tag="PC479_001850"
     CDS             complement(9289..10140)
                     /locus_tag="PC479_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2804397.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(10157..11077)
                     /locus_tag="PC479_001851"
     CDS             complement(10157..11077)
                     /locus_tag="PC479_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2804398.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(11187..12101)
                     /gene="murQ"
                     /locus_tag="PC479_001852"
     CDS             complement(11187..12101)
                     /gene="murQ"
                     /locus_tag="PC479_001852"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2804399.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(12200..13297)
                     /locus_tag="PC479_001853"
     CDS             complement(12200..13297)
                     /locus_tag="PC479_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2804400.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(13530..14876)
                     /locus_tag="PC479_001854"
     CDS             complement(13530..14876)
                     /locus_tag="PC479_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2804401.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(14965..15273)
                     /locus_tag="PC479_001855"
     CDS             complement(14965..15273)
                     /locus_tag="PC479_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2804402.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(15536..15934)
                     /locus_tag="PC479_001856"
     CDS             complement(15536..15934)
                     /locus_tag="PC479_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804403.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(15936..17096)
                     /locus_tag="PC479_001857"
     CDS             complement(15936..17096)
                     /locus_tag="PC479_001857"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2804404.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(17527..18546)
                     /locus_tag="PC479_001858"
     CDS             complement(17527..18546)
                     /locus_tag="PC479_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804405.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(18540..19220)
                     /locus_tag="PC479_001859"
     CDS             complement(18540..19220)
                     /locus_tag="PC479_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804406.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(19372..21279)
                     /locus_tag="PC479_001860"
     CDS             complement(19372..21279)
                     /locus_tag="PC479_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2804407.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(21280..22032)
                     /locus_tag="PC479_001861"
     CDS             complement(21280..22032)
                     /locus_tag="PC479_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804408.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(22348..23502)
                     /locus_tag="PC479_001862"
     CDS             complement(22348..23502)
                     /locus_tag="PC479_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2804409.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(23505..23972)
                     /locus_tag="PC479_001863"
     CDS             complement(23505..23972)
                     /locus_tag="PC479_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2804410.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(23986..24474)
                     /locus_tag="PC479_001864"
     CDS             complement(23986..24474)
                     /locus_tag="PC479_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804411.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(24488..25819)
                     /locus_tag="PC479_001865"
     CDS             complement(24488..25819)
                     /locus_tag="PC479_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2804412.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(25833..27398)
                     /locus_tag="PC479_001866"
     CDS             complement(25833..27398)
                     /locus_tag="PC479_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2804413.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(27568..28353)
                     /locus_tag="PC479_001867"
     CDS             complement(27568..28353)
                     /locus_tag="PC479_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2804414.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(28520..29719)
                     /locus_tag="PC479_001868"
     CDS             complement(28520..29719)
                     /locus_tag="PC479_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2804415.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         9900 a         5172 c         2995 g        11786 t
ORIGIN      
        1 tatcttataa gcaatgtaac tatatatctc taataactac ttttttaaca tgcttaaact
       61 atttataagc tattatattt atgtattaac ctaataactt tttcactaaa aaataacaat
      121 ctattcaaag ataaactata gtacttcttc tactttgttt caataaaata ctaaataaat
      181 ttcgtacatc cacaattttt caggctacac tataaatata caatatttat cttagttata
      241 agtttaagaa tatttttagt cttattttgt cgaatatctt tttaataaaa cttcataatt
      301 ctaagcatat ttttacactg tgttttcatg tacaaaaata atgcttacaa ataaacaaca
      361 taaataaaac ttaaataaga tggattctat tcaacatagt atccatctta ttcattcaaa
      421 acctaaaagg attttttata atattatata ttttaaaatc taagtaactc attatagtat
      481 caatttcact taaagcttac aatacactta ttaatcttgt tttatattaa aaacatctac
      541 aataacaatt taaatcttta agtgatatcc atctcacacc taaaggagta ggctctccgc
      601 actattctgt aattcttcaa tacaatcatt attatttttt atataattta attatatatt
      661 ttattttacc aaaaccagca tacataaata atacttaaaa tagattaaat aaaggatact
      721 aaaacacttt attgaggtat atgacttgaa atatatgaac taacttcatt aggagaaata
      781 tctaaaatag ttgcaaattc ttcatttaat aacctttcag cttctttcat tatattatca
      841 tctgcttgat gtgttttttt accaatagat tttacatact ctctgttatt atctatactt
      901 cttactaatt ttattaactc ttcacattta ccacctctga gtattttttt aaattgctca
      961 cttctcaatt tttcattatc tatccatgat gtatccatat taggaatatc atttattaat
     1021 gaaagcacat taattttaga aagtaacttt ctcattggta ctttttcatt atctacagga
     1081 atttttatta ctgtattatt agaataaata ggactcaata cataatactc tttttttatt
     1141 ccatttgtaa atcgttcatt tgttatatcc atgactttgc agactcctgt cataccatac
     1201 attatataat catcaatctt aaacaaaaac atcaccatcc ttaataaaaa aaatagtttt
     1261 gacaactaaa tccaccttgc caaaactatc tgctatatat tatattttaa cacattttga
     1321 gacttaaatg taagtaaaac ttatattatt aaaattaaaa aatacattaa ccattgatta
     1381 atgtattttt tagattttta taacaagcat atcaaaataa attaatacag cttatctcct
     1441 gtgaatacct ataaaattgg tgcttggcta ttcattgagt acccatcatt tcttacatca
     1501 tttatttcta atatttcatc atttgtttta accttagtaa gttttttaac tgctccataa
     1561 ggttctttta atgaaatttt atctcctctt ccagaaataa cccataaaat tttgtaccct
     1621 ctaggtatta cttgtagttt attttcacct ttaccatcag taaagtagac taacaaatta
     1681 atctttttat tattagcata ctcaaagact ggagaaaatc tagtacctcc acctatccta
     1741 attctatctt ttatatcttt ttcagttctc actttatata cacgtctaat ttcattatca
     1801 cattctataa ttgtaatttc atgattatag tttttcacta tactaagaac ttctttaata
     1861 gcttgtttaa actcttcatc actaatactt ccacttacat caagtgcaac tgctatttct
     1921 gacttatgac ttctaagttc tcccctcaaa tctaaacgat ttggttgtct tctatttctt
     1981 cttgttatag tcttcttttt attattttca actgttccca ttattctatt aagatataaa
     2041 ttccatggca attcaccctt actattttta agtgatgata tcatactttc taaataagca
     2101 ggaactttac ctttttgaga actgttaata aatttttcag taaattctct aagggtattt
     2161 tcatctatct cattggattc atcccatata tcatgagttc tttcggggct ataatcaatt
     2221 tctatatctc catatttatc actatcatct tcctcaccat cttcatccac ttccatcaaa
     2281 tctaattcag tctgaatctt ttctacataa tattcaaaag gttcatatgg ttcaagtttt
     2341 aaggaatatt ttaaatttac ccactctaag gtagttgcat atggtggtaa attgtttaga
     2401 aatttattta ctactatatc cattgccata ttaattgcta atgtactata ctttcctttt
     2461 aactcctttg ctctcatcaa atgcatagat aatatatgat gaatttcatg tttaattgta
     2521 gtctccattt gctttatgtc aagcgttaaa aagattattg gattaaaata tataatatat
     2581 ttagctccct taaaattcac acctgtagga ctacttatat caaatcttat ttctcttgac
     2641 atctgaaaca aaaaatatcc ataaaaatta tcttcttctt ccataagact taaattcact
     2701 ttatctacaa gactaaaaaa ctcatactta aaatcttgtg gtatatctat atcaaatttc
     2761 tcattagcac gatttgcctt tagcatagaa tgagtattta taatttcact tgctttatta
     2821 taaagctctt ttgcttgttt atcaaaataa ttttccatac attatcccct tattaaacta
     2881 taagattcaa aatatgattt tacaaactct tcattttcta tagctttttt atatacttca
     2941 atataactgt ttctaatatc cttcataata cctatcatta aatctacagg atacattttt
     3001 aaaaattcaa taaatctatt aatatagtgg tttgattcat atttatcatt cttaatattt
     3061 aattctaagt cttttagaat gttcatagct gatagataaa gtcttgtatg actctcattt
     3121 tttactcttt ctacaataga ctcatcaata gattctccta aaaaaacatc ttcataggat
     3181 attaatggtt cagagtttga ttcaataaaa cttataaatt cttctgcaat aactttacct
     3241 acattaccct ttacaacatt taaaaatact gctctaggaa tagaattatt tttttcttta
     3301 taaactttat aaattttaga tactctctca taacttctag gagtggctct tacatcatct
     3361 tcatttattt tatgcagata ttctggaaaa gtggaaataa actcgataac ttttctttca
     3421 ataccctcat ctattgccca ttttatccat tgtgtatggt cactctccat atttaaccaa
     3481 acaaacctat tttcttgggc tgcatccata tctaccactt gataatcaaa atctgaacca
     3541 tatttacttg atggattcat tgctgctaaa atctttacat catcatgcag tttatatcca
     3601 ttaatttctc tatttaaaat taaattcata agttcttgct gtactgtatg ctcacaacga
     3661 tttatctcat ctataaataa aagaactgtc ttacttttag atatctcttc atcaatttct
     3721 cttaatttgt tatgaacagc atacactgtt gttttctttt cagtcttata tccattagaa
     3781 ttaactccta cataagattc tatagttgga agaccaccta tttcaccctc tttaagtaag
     3841 tttccatcaa taacaattaa actccaatca ttttctttgg caagcttatt tgctaaagct
     3901 gtttttccta ttccactttc acctactatt aaaggtactt ctccagtgga taatattaag
     3961 tcaacgcttt ttaatgtgtc tataaaattc atttttctct ctcctacata atctttaatt
     4021 tctcatctac agctattttt tttgctgtct tacttccata tttataaata aacataattt
     4081 tatctattgg tatactttcg ctatttctat ctataacact acaattcaaa aaatctctaa
     4141 tatacttttc ctcaacatct tctttggata atacttcttt taatgcactc tctaattcat
     4201 ctttagatac ttttttcaga atgtacttaa tcactaaaat attcactttt aaaaattcca
     4261 acattttatt tttatctaaa tcagttataa agctaatagc tgcttcatta ttttttatag
     4321 ctataagttc tgcattataa ctaggtgaat ctatatatct caaggcatca taacttattc
     4381 tcactgcctc ctcttgtaca ctttcataag gttgttttat aaactttatc gaatcataat
     4441 tttttcttac tgctaacagt tgtaattctt cacttggatt tttagcatat ttaattgccc
     4501 atcctgcttg atttatagcc atttcaataa ctttatcagt tgggtttttg atataatcca
     4561 gtatactcca tccctcattt actgctttta tcatcatatc ttctgttgga ttatctataa
     4621 atttaattgc tctaatatta ttatctattg ctagttcttg catctctctg gttggattat
     4681 ttatatattc tagtacaagc ccattttctt taatagctaa caatttcatt tcatcagttg
     4741 gattatctat tgtagctata atattgggat tattttttat catttcaata actctcaatt
     4801 tttccataat gtgcctttcc ttctataatt atttttatat ctatattaat tataataatc
     4861 tattcctatt tatataattg tttcttatta taattaatat actttttaat caaatttaaa
     4921 cagaataatt ataccaatat ataaaaaaat ttctaaaaaa caataaaatt aagttataaa
     4981 caccaatata tttctattat ttcaataagt atacaattag attagaatct aacgctagta
     5041 ataaaaaatt actttcccaa aatagatata agcctcattt tatcgttcaa ataatgaaaa
     5101 tatatttgat ttcatgcaaa aagagtgcct aatgaactaa aaagttcatt ttgagacact
     5161 cattttttta tataaattta tatagttttt aatcgttcgc attcaaccac tcagttaaga
     5221 cttttatata tttctcattt tcttcaacga atggcatatg tcttgaatac tcaaataatt
     5281 cccacttact attaggtatt ctatcataca tagtctttgc aatatatggt gaacataaat
     5341 ctattgctcc actagttact agacaaggct cttttatttc atgtaatcta tctgtaaatt
     5401 catatccaga aagagttcct gtaggagaaa attcattttg tccccatcct acaatatatg
     5461 cttcagaacc tgatttttta ggtcttctca ggcactctgg agaatcttct gtcacttcac
     5521 ctgcacaata catttccata aatctttcta atgcatcatt atattctttg ctagagtaat
     5581 ctccagtatt tactgcatca agtaatgctt tttggtcaac ctcactcata tatgatattc
     5641 ttcttttttg ttctttctcc caaagttttg cagaagaaag agtagatgaa aggatgtaag
     5701 acttaatacc ttttggttta tattcaatag cataccaaat agcctgcata cctccccaag
     5761 attgacctaa cagatgaatt tcatcaagtc ctagatgttt tcttagttca ataagctcct
     5821 ctatccaagt atctgcatta aatagctcag gatgcccttc tacaaatgaa tttccacaac
     5881 caatttggtc atacattatt acttgtctac cactttcagc tatcttatcc aatacctcaa
     5941 aatagttgtg tgtagaacct ggacctccat gaagtaatac taatggcttc ttcccttccg
     6001 tattttctcc tactatacgg taatacgttt taaaaccttt aaaaggcata tatccttcag
     6061 taattttcat gtttatctcc tccaatttgt aattataaac aataatcaag ttcattaaca
     6121 atactattta aaacctctta tacatatttg attatgtatt ttaatcttta tacctacttt
     6181 tataaatagt ataacataaa agttaaaaat ctctatacta aaccaattat aaaattatat
     6241 taactatata attttatttt ttaataatag tatatacata tttgattatt ttcatttgct
     6301 atttaaaatc aattttaagt tttcttctta taataaactt cctctttgtg gtctcatgtt
     6361 agtctctaaa tttaaaattt tacaaaaaat tttcataaaa taaaattata ttgattgtat
     6421 ctaaacaaat agggcaataa aataattaag tatagttact tacctttcaa gcaatattag
     6481 ttttatgtta gaattttatt atattgattt acaggaggtt tgcatttgaa taatattaat
     6541 tcacttaaag taataaaatt atctcttgga tgttctcttg ctatatttat tgcttggcta
     6601 ttaaaattag aatattcaat ggttgctgga gttatagtac tacttacagt aaaggacact
     6661 aaaagagaaa ctcttaaagg ctccattgga aagatttatg gttttctttt atgtactatt
     6721 ttttcatatt tatgttttaa tattttagga tataatttga cttcattttc aatatttata
     6781 tttataatta taccactttg ttttttgtta aatattcaag atgttattgc tatgtgtgta
     6841 gtcatagcat cccattacta ccttcaagga gaaacttcta tcaaatggat tttaaatgaa
     6901 gctggaatat ttgcagtagg aactggaata ggtgtcttaa taaatatgta tattccaaca
     6961 aatatacata aaatccatga agttcaaaaa aagttacagg aagaagtgag tattgtactt
     7021 atagacattg cagatattat tgtaaatcct aaaaaagaaa atggatatag tagagattta
     7081 tatacattaa attcacttat tgacagcagt atttctgaaa cttacgataa tattaataat
     7141 actttattaa gtgatactag attcttctta gagcatatgg atattataaa aagtcaacgg
     7201 gatatcttag aaaatttgta tagttatgta tctcaactaa attctacacc accacaagct
     7261 catattctat ctgcttttat acataagata ggttatactg aatttgaagc tgaaacaggc
     7321 aatcttcttc ttgaagaatt aaagagacta atgcttagta tgaaaaacca gcctctacct
     7381 gttgacagaa cagagtttga aaatagagct attttattcc tatgcttgac tgagttaaaa
     7441 cagtttttag ttaatcgtaa acatgctcag atgcttagag ataataattt ttataaataa
     7501 aaaggggaga aatctagcag ttgacctgac tacaaaatat cagatataat attaaatgcg
     7561 cccctagtaa gagggcgcaa tgtttataac ttattctact gtcatgatta aatcattatt
     7621 catatcgaac atacattatt tgaccaagtc caatattagc tttttcatca gtgatttcta
     7681 ttttaatagt cacatcattt tttttacagt acttcgctaa ttctgcacca gaacgatttt
     7741 taccactaaa atctaaagaa aatggcttat catatttaaa aggatattca acaggtttac
     7801 cctctggtgc taatgcaaac atcccatatg gtgtgttcat aaatcctatt acagcaatcg
     7861 taagatttga tgggtctaaa tttgattcac tacctgtaaa tgtaattgta tttactgatg
     7921 aattaactac actacatcga acaccttcat acattacatg tttttcattt atatcttctc
     7981 cttttttccc aaaatttcca acagcctttg ccccttcttg atgtggtaac atagtgtgct
     8041 tcccaggtgg tgctggtttc tgtgtctgtt ctggttttac cgtctgtact attgcaccaa
     8101 cttttggata tgctgtctta tcaaatggta atgggtggct aagactcttg tatatgatat
     8161 cctttcctgc tgatttatta gcatcaatat ctacatgata caaacgccca tcaccaaaac
     8221 ggataagatt ccactggtgg tcatttgatg tgctgtgtac ataataagat tcaattccca
     8281 tagcatcaca caaagctttc atgctcctag ccattcctga gcatgcacct tttttagtaa
     8341 ccaatgtata ggcagtattt gctccctcta ctgcattcca gtcaacatat gttgtgttat
     8401 ttttaatgtg attgtaggca actaaaatct tagcatctgt ggtcatattc tttgtgattt
     8461 tcttgttcat aaaatctgct gttgcttgtg ctactgagtt tagttgggct tgtgacagct
     8521 ttggaccata tacgctattg gttccagtga catagctttt tggtgttttt gctgttacct
     8581 ctttggctgt cataccataa actgtcatac tcatacctga gattaaaact attgttgtaa
     8641 tcaataactt ggtagctaac ttcctaattt tcttcatact taatattctc ctttataaaa
     8701 cattgttact tcctattctc taccattaat aaaattaatt taggatttaa aaaggtggat
     8761 tgtcaacatg tatatttctc cattcataca ttcgtgaaat atttaaattt catggaaaat
     8821 ctaatagata ttttctagat aatttgtata caatacaact cgcaaatcag ggtgtttgct
     8881 gttagtttga gaaaatgatg atgattaata cattcggaat ctttgaccta taataaatac
     8941 cattgtcttc aatcattaat aatggtaaga gtaaagagaa ttatcaataa tacagttaaa
     9001 atcaactttg tatacatttc atacattctc tcccaacaac attatacttc ttataatttg
     9061 tatattcaat acgttttctg ggaaaatata ggatatatct ataaaaatat aaaaaaggag
     9121 ttgaacttaa aatttgatta aaaaaaatca attttaagac aactcatttg tattataaaa
     9181 atgctatata ttattattaa gtaagtcaat attttagata tttatctatt tcttattgac
     9241 ttacgcttat agtctaactt atttgtttag ttgatatata acctagtttt aagcattatt
     9301 aatatttaaa taatcaagta tcctttttgt ttcttgtagt ttctgttctg cctcatttaa
     9361 gttttcttga attatagaaa tatatattat atctatcaac gtaagcattg ctattcttga
     9421 agaaatagct ccttctctaa gccttttttc tatatttggt actactagtt ttaaatcact
     9481 aatcttacta agatgattgt cactatattt agttatacaa atgaccttac atccttttaa
     9541 ttttgcattt tctatagact ttattacttc ttttgattct ccagagtaag atattgctat
     9601 agctatatct tctttatcta catttgaaga taccataagt tgtgtatgac tatctaaaga
     9661 ggtaaatgcc tgtttattta tccttaaaag tttcatctgt aaatctagtg caactaaagc
     9721 cgaaccacct actccaaata tatatacatt tttagccttt attatctgtt caactgcttc
     9781 ttttattata tctttttcat tcaacaacct tgtttggttg atagtctcta tgtttttgtt
     9841 tgtaattttg tttataacac tatctatatt acaatcatag ttttcatcta catatgtgta
     9901 ttcattttct ttgcccatca aatctttagc tatctcaatt tttaaatccc caaaacccga
     9961 atatcccatt tttctagaaa acctaactat acttgctgta cttgtttttg agtttgacgc
    10021 caattctgat acagaaagct tacttatagc ctctgtatta ttaagtatgt aatcagctat
    10081 ttttttatct acttccgtta aatcttcata tatctgtttt aattttaaaa tgcaactcat
    10141 actctatcac ctctacctat ttactataat atttcttctt agcaagtaaa catgctccta
    10201 aatctggagt caacactggt ctcactatac caaatctcgt tttattcaaa tattttttaa
    10261 gtggctctaa tattaaatct cctgacttaa acactccacc tgaataagat acctttatct
    10321 ttgtaccttg ttcaaaatga tgctctaatc ctattataag cctacttaat tcataagctg
    10381 catcatcaaa tattttacta gcagtattat caccttcaat aactgctttt aaacatatcg
    10441 tagcaaactt tgctatttct attctatctc cttttatttc atcatttaca tattttatta
    10501 tgtcataatc attctctata ttaagatgtc tttttattaa atcatataat tttgtttttg
    10561 aacaccttcc atcagactgc attgtgaaca tttttaaggt agccattcct atataatacg
    10621 ctgaaccttc atctcctaat acatagcccc atcctccaca tctgtgaaga ttcccatctt
    10681 tatctaaagc ctgtgctatt gaaccagtac ctgcaattat atttatacct ttttccccat
    10741 ttaaagaacc tgctaatgct atttctacat cattacctag agtataattt atatgactat
    10801 atgcattttt tgttgctact tctaatgcat acaatacttc ttttattttt ccatatccag
    10861 caagtccaac aaatgcatac gttatttctt ctacatttat cttagcatct ttacaaactt
    10921 cttccaaccc agttattaat aatttcgtta gattatcaaa tcctatttga ttataatgac
    10981 aagttccttt ggttatggtt cctactatat ttaattcttc atctactaat gtaaatgttg
    11041 ttttcgttcc tccaccatct acacctaaaa aatacatact atcctcctac aatatcttta
    11101 ataattcttt caatacttaa tatctaaaaa taaccttcca atgctgtttt tttaatattg
    11161 aaaggttatc agtactatta tttattttac aatttttcta ttgattttaa agcttcactt
    11221 atataacctt cttgtctatc taggatactc tttgcactat ccttatctaa accacttttt
    11281 atcataaata tagccaattt aacgctatta tctgcttctt ctaagaattt ttctgcaaca
    11341 tcatatttta catttgtagc tatcattatt attctttttg ctctttcaac caacttttca
    11401 tttgatgtct taacatctac catcaagttt gagtatactt ttcccatctt aaccatagaa
    11461 gctgttgtta acatattaca taccattttt tgagctgtac cacttttcat tctagttgaa
    11521 cccattatta cttctgcccc tactataatg tttattggta aatccgcttt tttactcatt
    11581 tcactattcg gattcatagt tattgatagt actgcacacc ccaattcctt agcatactcc
    11641 attgcaccta taacataagg agtccttcct gatgcagcta taccacatat aacatcctta
    11701 gatgtaactc ctctatctat tatatcttgc tttccaagtt ctttatcatc ttcagcacct
    11761 tctattgctt taaatattgc ttgatttcca ccagctatta tagcttgaac aagctcaaaa
    11821 tccactccgt aagttggagg acattctgtg gcatctagta ttcctatcct cccactggta
    11881 cctgctccta tatagaataa tctacctccc tgtttcattc tatctactat tatattaaca
    11941 gcttttgcta tactctcctt ttgttcttca acagcatagg ctacagtttt atcttcgtta
    12001 tttattaatt ctattatgcc taatgtatct tctttatcta tttgcaatgt attcttattt
    12061 ctaccttctg taactaaatt ttctaaagtt tttattaaca tatcatttcc tcattttctt
    12121 aatattaaat ttttaaacac attttaattc caactttcat aattatattt ttataataaa
    12181 ttaatatttt cataataaat tattttgttg agggtttaaa attaaacttt tgccatggtt
    12241 taatataatc taatataaat aattcttctt ttcttatata tcctactaca ttcgatttac
    12301 cactgttttt catatcagtc aaggctattt gtaactcccc tgcataatga ccatattcac
    12361 tactttcaat tactatatct cctcttttta ttattttagg agtattaaat agttcaaatt
    12421 tatatccttt atatttaaca cgagattgag tagaccttat cagactatca gatatgtccc
    12481 ctctattaaa atgtatttct tcaaagagta tcttcttctc aacttctcgt atattttcct
    12541 ctattgctac atcaaatgta accatatctt ttctcataga acctaatacc tttagctcct
    12601 catatgttgg ataacaattt gatattatta tatcatttat attattcata gctattatat
    12661 gctttacttg aatatctata ggtagatttc tgtgcatctc aattgttgga agcccatcag
    12721 ttacaggcca tggtccaaat gtagtttcat tttgacttgt tacaaaaact gctgttctta
    12781 atccatactt attaaatctt tctgtacatt ttataaagtg ttctaaattt aatccactat
    12841 atctatgtgg ataaaagtta tgacatccta tcaaattttc tttatttggt tggtagtcca
    12901 ttattgtatc tatataatta gtattattac tcatattgat ttctatttta agattttgag
    12961 gattaaaagt cattaatgcc tcttcagaac ctgtaaatcc catatcaagc cttattccgt
    13021 ctgccttcgt ctttttaaaa aaattcaaat ctttgtagct tatttctaat tcaccaaata
    13081 ctctgggatt tacatcaagt attacctcaa aacctttaaa cttagcgtat tcatttatac
    13141 ttttaaattt tttaattata ttttctttag tatctttaac tgataataaa catgaaaata
    13201 tacgtgtaaa tccattttca gcagctttat ctatataatc atatatttcc ttagatgttg
    13261 ttttttctgg ataaattgat attcctaatc ttcccatctg tgtcatctcc ttatatccta
    13321 tatgtgttta aatttatttc gaattacacg aaactttttt aaaaatttga tgaaaaaaag
    13381 tttcatatat atttataaaa aaagaaaagt ttaccttttc tttttttata aacaatcaac
    13441 aaaatatctt cgttacttat ttcataactg ttatactttg aaatttatac taatctaata
    13501 tagtttttaa tttccaatat agtttctaat taataagctt cttgctcttc aactttttct
    13561 tcatgaagca acctcttatc gtacatcttg acaaatggat agtaaattat tattgatata
    13621 gctatacata ataagttaag aactgaagct ctccaatctc caccagtggc taaatatgcc
    13681 cctactggtc ctggtaatgt ccaaggagca gtaactacta ctctggatac taaattagca
    13741 ctcatagcaa accatgaaac tgttgccatt actattggtg ctacaacaaa tggtattatt
    13801 aatattgggt ttaatactat tggcgctcca aatattattg gttcatttat attgaatatt
    13861 gctggtatta cagttgcttt tcctaaagac tttccataag ctgattttgc tctaaatgca
    13921 agaagtaaag ctaagcctat tgtagctcca gaacctccaa tccatataaa ccattgaaaa
    13981 aagggttcag cagcaatacc tggaactgct tgacccgcag catatgaagc tgtattagca
    14041 tctaataatt gtatccataa aggtcttgcc aatgaaccta ttattgatac accatgtatt
    14101 ccaaatgacc aaaaaaacgt tattaaaaat attagtataa ttactgatgg taatgtgtca
    14161 gtggctgaaa ctaatggagt tactaattta gctatgaagc catgccaatc aaaccctagg
    14221 aaataagtaa caatagccat taaaattatt attaatgcgg ctggtgtcag agcttcaaaa
    14281 gaccttgtga ctgatgctgg aactgcttct ggcatggtta tcttgaattt agatttgtca
    14341 gtaaatctat aaatctcaac acctattata gatgttacaa tagcaacaaa cattgaacct
    14401 ccaccaagat tagccattgg catttgccat ccttgaggaa ttgaattata ccaagctaaa
    14461 agttctggac tcttttcaac tatctttaat aaatcagggg taagaccctc tattgcagta
    14521 ggaactattg tcaaaaggaa tgtagctgta gctaataaac ctcctgtcaa tccatctaac
    14581 ttatatgact ttgccaaact ataacctatg ccaaatgtag aatatactga tataataaaa
    14641 aatgtcattc tatatggtag taatattgta actgcatttt cctttagata ctgagttacc
    14701 ccccatgaat ctggtaatgg aggaaatgct aatatcaaga aaaatgaccc aactattata
    14761 agtggtaatg aagctacgat tccatctctt acagctctta agtgacgttg ttctgccaat
    14821 ctggccatag aagttgataa tttagtgtct aaaaattctg taaatttatc tagcatcttt
    14881 tctctcctta attaatattt atttttatta tcattaaaat ttttataaaa ttaactttct
    14941 tctaatctaa caacactgca actactattt actcaacagc tcttgaactt gttttaataa
    15001 ctttggtcct cctaatggac tataagcttg tgcttgtatt aatgcacatg gtacactaac
    15061 ctcatcagca aaagctttta aatagtcaaa tctatgtcta acttgtggag ctaccatagc
    15121 aatatcccat ccatttttaa cttcatcctc aaactcttga gtaccaacag ccaaaacatc
    15181 tatatctatt ccattttttg ctccttcttt ttgtaatgca ttaactgcta tagcacttga
    15241 catccctgat gaacaaacaa ataatactct catattgatt ccccctgttt ttttatttct
    15301 tttcaatcat tatagcatgt atttttgatt acaggaatac gtttgtatat atttttttca
    15361 tattttacat tttaaattgt aattttattt catacattta tatcttgctt taattgcttt
    15421 atttatctaa tcattctttt gtctaaaatt acacactact taaaatactc atatcttttc
    15481 ttaaaaaaga aaaaagacta tcttattatt aacataaaat agtctcaata cttttttaaa
    15541 tatgtattcc tttatatatc cagaagtaac ctattattgc aaatgataaa gcaaataaaa
    15601 tatacagtct aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag tatcttgtat
    15661 atgcaactgc aaaagatgta attaatgaat ttcctataac aactccaaat ctattatcta
    15721 ttccaaatat agataataac aaaggaaaaa ctacagtact tactagagat gaaactatta
    15781 atattaatcc acttttaaaa cttatagttg gaatattata atctgttctt tgatttttct
    15841 ccatttgttt cttagtattt ttattacttt ttgtactttt tgcattttta taattattat
    15901 ttttctttct tttctttttt ttattattag acatgtcatt tacctccaaa aggtttaggt
    15961 gttatattcc ctaaaataac tttattttta gcccctgttg cagatattac attagaaaaa
    16021 gaattattta atgtttcatt gcccaagatt acaaatgcca aagcctcctt tgcatcagaa
    16081 gaatacccat attcatcttg agttaatact tcaacctcta ataataactc ttttatatat
    16141 cctattaaag ttagattatg agctcctcca cctccaatta ataaagtatc tacattaacc
    16201 tttggaagta tgaagttttt ataattataa tatatagctt ttgccgtaaa catagttact
    16261 gttgctatca aatcatattt atctacatgt ttatatttgt taagcatgtt atccaaataa
    16321 acttgtccaa atacctctct tccagtgctt ttaggtggcg cttgactaag atacttatga
    16381 ctcatcaaat cttttaacat ttcttcatta attttacctt ttgatgcaaa gtatccattt
    16441 ttatcatact ttctattaaa taatctttga catactccat ctattatcat atttcctggt
    16501 cctgtatcaa atgcaaatac atcatctatg ttacatgatt taggtattat agtaatattt
    16561 cctattccac caatattttg aagtgctaaa tttttattgt tatcactata taataaaaat
    16621 tcactatatg gtactaatgg agcaccttct ccacctgctg ctatatccat tactctaaaa
    16681 ttagatatta cctttgtatt tgtttcatat gctataactg ctggttcacc tatttgaagc
    16741 gttgaagata caaaattatt ataacttctt ggtatatgaa agattgtttg accatgagat
    16801 gcgataaaat ccaaatttgc tatatgaaag tttgctttat gacacactga ttttacagct
    16861 ttactaaata aatatccaag cttaaaattt aagctacaga ttaaatcaac actcgattcc
    16921 tcttctatac agcactttct tatttcgtct tttatatctt taggaatttc tagagttata
    16981 aattctattt gcttaacttt agtatttcta ccattcccac taatttcagc tagtactgca
    17041 tcaactccat ctaaggaagt tcctgacatt aaacctacac tatacatagt tatcatcctt
    17101 ttaagtttat agttattcct ccccaaatca aggtattaag tttaataatt ttagaattaa
    17161 tcttatttca tctttaatta cgataattta ctcttatgaa attaattgag ttaatcttat
    17221 acacgttttt taccttagct aaatcttaca ttaatttatt ttcagtgtca atgtaatacc
    17281 atgtaatttt ttttcacaaa caaaacatat tctctctcca aaaaccatat taaaatagtt
    17341 actacaaata aaaaacatac caggtaccta tctgatatgt ttttcatata taataattat
    17401 tattttataa tatagataga gcatcattta aggcactaat taaatcttca ctattttcaa
    17461 gaactaaatt tattcttatt gtatatgtcg atttgtatat gatgatagat tttatagtct
    17521 aataacctaa ctatctaggc ttaaataatc cgatatatca taaaaataga tattaaaaat
    17581 tgtatatgaa ccattttccg aacatacctc aatctcatgc cctaaactat ctatcatttt
    17641 ctttacaaaa tacatcccca ttccagtaga cttatacaat tgatttcttc catttttccc
    17701 cgtaaatccc ttgtcaaaaa ccctagatat atcttcctca tttataccta taccaaaatc
    17761 ttttatagat aataccactc cattttctaa cttttttgaa ttaaattcaa tcttaccaac
    17821 ttcctttgaa tactttatag cattattaat aatttggtct aaaacatatg acatgcattt
    17881 agaatctgtg taaacatcat tttctaattc attcaaacta acttctacat tattttttat
    17941 aagcaaaaat gaattatttt ttatagacat ttttatcaat ttttctaaat taaatttatt
    18001 tataaataca tcttcaaaca tagtgttgct tctactacta tacaaaatag agtttactaa
    18061 aaaatttatc tttgcaacct gattcttaat atccaaacta tcttctatgc tacttaacct
    18121 atctgtaatt atacttaaag aagatattgg aatttttatt tcatgaaccc atttagctat
    18181 gtactcatcc aattctttta atgattgttt ataagtctct atgtcactat tattttttat
    18241 ttttaagttt tctataatat cttttattag attttcttca aaagattgtc catcaactaa
    18301 ataactgtct atttcaccac tattttctat acaattatat aaatttgtgt aactatttct
    18361 aaaatttata taatctataa caaaaaatac cattattata aacagtacta aaaaatctaa
    18421 atatattaac tctgaatatt tatttttaaa aatatctata gcaaataagt agatatttat
    18481 tatactaact acaactaaag gtagagcaat atacttagac ctacctttca aatattttac
    18541 tagcattgta ttatatatcc tacccccttc ttagttttta taaaatcatc aaaacctagt
    18601 tctttaactt tacttctcaa tctattcata tttacagtaa gtgcattgtc tgtcacaaac
    18661 tcatcattat cccatagagt catcataaga ctttcccttg atacaaccct attcttattt
    18721 tttaataata atgttaaaat ttttatttcg tttttagtca actctatact tttatcctta
    18781 tacttatgtt ttataatccc tttccccaca tcaaacatca aatcttcaca atacaaaata
    18841 ctattagaat cactataatt atagactctt cttattatac catttatttt ggtaaccaat
    18901 atttctatag aaaatggttt ggtaatgtaa tcatctgctc cattatttat acccattatt
    18961 aaatccatat cagaatctct tgatgataaa aatattaatg gtacatttga aatagctctt
    19021 attttttcgc accagtaaaa tccattatat aatggtaaat ttacatctaa aagtactaca
    19081 tctggttttt tatctataaa ttcatctaat actacctcaa aatcatcaat taattctact
    19141 ttaaatcccc atttttcaag tgctaatgca atctcttttg ataaatcaat atcgtcctct
    19201 actaataaaa ttctatacat ttgtatattc tccattatcc taatatttca ctatgctatg
    19261 aattattaat atcataacat attttatatg aatttattac ccaaattatt tagttaatca
    19321 acaaggagac tcttattaca agaatctcct ttctctttat taatatatca actaattttt
    19381 atacactaca ctcttaaact tattaacaga tactgctcca tatattatga atattatcaa
    19441 acatatcact aaacttagta tatatatacc aaataaatcg gtcatcaata aatctcctgc
    19501 taatctcaat gcaaaataac tagatatagt tgcacttaac attggcaaag tataaaacat
    19561 agctacttct ttaagtatag ctctatttat atctttttct gtagtaccta tttttaacaa
    19621 aatcttatat ttctctttat cttcatatgc atcactatat attttcatat atattatact
    19681 tccagctaca attgcaaaga ctaagaataa aaacacaagc actacatatg caaacttcat
    19741 ccattgactc tcaccttgag ctttatatcc attttgaata tacccatttt ttaaacttaa
    19801 attcttttca atattgttga acattacttg actattttct tcttgaaata cctttgcacc
    19861 ataaaaatac acttgttgac ccttatctct tagtttaaaa tatgtatcat catttaccac
    19921 tataatactt tgattaagtc ctgtccctag tactggaact tttacttctc cccttgaaat
    19981 attatataac tgaccatcaa gttttatctt ggcttctttt gtagctattg atgctatcgt
    20041 ttgagggctt tttaactcta ttacattatt tccactaact aatctttcac catactcttt
    20101 taattcttta tcattgccat tcactttaag agtattacaa aaatcactat atttcattat
    20161 agtatctgtt gtatcataag cttcttttat atctgttttc tttaaaattg ttagttctgg
    20221 atttactgag tactttatct tattagtatc ttttattata ttctctattt gttttttatt
    20281 cacttcatct ttatccattg aaactatgct aatagtgtac acatttcttt gttcttgggc
    20341 attcttatgt atacgcttca tcgcaactga agcaccaaga gttgatattg ttgtagttat
    20401 aattatagct attatagcat aagttttata gtttttattt aatcggtagt ttatattgtt
    20461 tattgcaatt atattatttc cattaaataa tattttttta tttttgatta taatatcaaa
    20521 tataacaggc ataactgatt tgaataatcc ataagtacct actattatta tacctataac
    20581 aggtagtaca ctagttatat ctccagttct tatattggta gaaagatagt agccatatcc
    20641 tattactata attgatacta tagctaatat atatatacct attcctacct ttggtatttt
    20701 ttcttgtttt ttagatatat tgattagatt tattacacta cttctacata ttgttataaa
    20761 accttttaca gccattatta taaatataga aataaagatt aaagtagcat atagtatagc
    20821 ttctatacta agtccattag atacctttat attaaatcca gataacttta ttatcactac
    20881 ttgaaaaaat ctagatgtta ttatccctat aattattcct gttatacaag aacttattcc
    20941 tataagcata ttttctacaa aatatatttt tcctatagta tataaatcta atcccataaa
    21001 agacaatatt ccaatttctt tagacctatt cttaaaaaat atatttgttg cataccatat
    21061 aaagaaaaat aaaaatactg atagtatcat tattacagct actaacactg atagtgtaaa
    21121 ttttttattt tcatcttgta acacatttat agcatcacca tatcttaata attcaaaatt
    21181 agttagcaca actactgaaa aaaccataga tataaaaaat gctagataag ttcttatgtt
    21241 actcttaaaa ttattataag caattccaag tatactcact tacatctcac ctcccataga
    21301 ggctagcata tccataattt ttccataaaa ttcttttcta ctatttcctt tgtttaattt
    21361 acactttata tttccatcat ttatcatata tacttcattt gaataacttg ctgttagagg
    21421 gtcatgtgta atcattataa tagtagcgtt attttctctg tttatttttt ctaatgattc
    21481 taaaagttca tatgctgatt tagagtctaa agcaccagtt ggctcatcag caaatatgac
    21541 tgttggattt gtaattaatg acctagctgc tgcgcctctt tgtttttgtc ctcctgataa
    21601 ctgatatgga tatttatcta aatgattttc taatccaaat tttttagcta tagaaaatac
    21661 cttagcttct atctccttac ttttcttttt tcctaatgct agaggaagtg ctatattgtc
    21721 cattagtttc atattgtcta atagattata gtcttgaaat ataaacccta ttttttctct
    21781 tcttaatttt gatagttcat tattttttac ttttgatata ttaattccat ctagtattat
    21841 ttctcctgaa gtttgtttgt caagtgtaga cattaaattt agcaatgttg tctttcctgc
    21901 accagatgga cccattattg atataaaatc accttcattg actgtaagag atatattttt
    21961 taacacattt gttttaaatc cttttattcc ataatctttt gatatatttt ttatttttaa
    22021 tatttctttc ataataatag cctccagttg ctaatttttt aatatatatt tattttaaag
    22081 gttttatata aataaatcct taagtaaata ttacatttta actgttagca ttacactttt
    22141 gtaacatatg gaagactatt ataattatag tagaaatatc ttaaaataaa agcatgattt
    22201 acttctttgt ataaacaaag aaaagaagct cctaagaagc ctctaatcta tacaattatt
    22261 cttctctata aaaaaccaaa atacacattg attgtggcaa atacagatac tataaacaag
    22321 tgatattact actatatatt aaaacaatta ttcctgaaga taattcaata agactatcaa
    22381 gcaataagct aagcatccaa aaagacttaa taccattaat gtataattat acccaatatt
    22441 aatgctcaaa aaaccagcaa cagttggaat aatcagagat gaacctccca taaatatact
    22501 taaagctatt aattggctac tacttctaag ttttttagga gaaacttcaa atattaatct
    22561 ttttgaagta attaaaatta aaggagtaga aaacacttga aaaacacaca gtataataat
    22621 gatatttgaa tcatttgtta aagcaaataa tagaaactga actgttatca taatagcact
    22681 taattttaat aagtttaaac tgctatattt ttttaataaa taagaaccag ccagatatgt
    22741 tggtatctct aacaatgact gtaaagacca ctttaaagat atctgagaat ttgtagcatt
    22801 taaagctatc attttatcta ttacagtaca gttatttgct accaccattg agtataataa
    22861 gaataaaata catactagta agatatattt tttatctttt aataattcaa taatatcttt
    22921 attagttaca tttattttct tattaatctt ttcaatatca ggtaataata gtatatttat
    22981 aataagtaat actacaatta aaccaatcgc tacagataat ccaaaatatg agaatctaag
    23041 aatcaagtaa gatgctacca tacttccaat agcccatcca atcgacccaa aagtcttaat
    23101 aaatgacaaa taattattaa tattatctcc acaacctatt atccatgtat catataaact
    23161 acagcaagta tttaataatc ctcctgataa agcaacaagt aataagtata ttataaaagt
    23221 tgcagattca gtagaaaaca taataactga tgcaattcca taaactgata aactaattaa
    23281 aacaattttt ttcattgttt gatacttatc agctaatact ccaaataata attgaaacaa
    23341 tatattggtt attgcatagc tcgataatat aattcctctt tcaaacgctg agtaacctaa
    23401 ttttgataag aaaggtgtga attttgttga agcaaaggca aaaataatat atgagaaaaa
    23461 atagaatatg ataaatttaa atttaatcgc tttattaatc ataatcaatc aaaattgata
    23521 actttaatac cactatttcc ttcctgattt aagtttgaaa gatgatattt taccgtatta
    23581 ggattctcta caataactaa aggaatatat aaagtatttt caagtaactt attatcaatt
    23641 tctactaact tttcccccat attaacttct tgaccttgtt taacttctaa tttaaagttt
    23701 tctttttttg ttgttgcact atccaatcca atatgaataa gaatttgaac atcattttgc
    23761 aatttaattc ctatagcatg gttagtttga gcaataaaac ttattactcc tttagtcggt
    23821 gatttaagaa tattgtcctt tgctatcata gcaaatccat ctcccattac cttagttgaa
    23881 aatactttat caggaatttc tccaagatta attatttttc catcaatgta tgaattaatt
    23941 gtaattgctt ctttttttct tttaaagaac attatagaca ctcctctaca taattaactt
    24001 tattctaatt atcattacaa ataacgatat taagcatact gcaattgagg aaaaaagagc
    24061 cttatagctc tttccccatt gtagttcttt aatactgctt atgatttgtt taattccaac
    24121 aaatttataa atataggtaa acatgataac aatagttatt gtgtaaattt ggaatatctg
    24181 ccctaataag actaaataga aagcaatcat gttttctttt atctctttat tcttttttat
    24241 attgtaaata acatatgcac tgtatactgc actaaagtta gagattaaat ttaaacttgc
    24301 attaacatca ttgtttaaaa gttgtgaaaa tgaaatattt gtaaataatg atgtcaattc
    24361 taaatacact atatataaaa caggtaatat tagaaaacaa accataacca ctgaaagaaa
    24421 tatattttca tcttttaata ctgctgtgat attttttatt ttatttgtta tcatgttttt
    24481 gttctcctta tttaaaagtt ggccagaaat ccttattagc cacaatcatt tcatctaaga
    24541 tttcttttgc tacttttgca cttggaacag ttttactcat agtaaacgac tgccataatt
    24601 tttgataact tttttctatc catgcttcta cagctaattt ttcagaagca acttgttgtt
    24661 ccattaaacc tttttggaaa gttggaattt tacctataga cataggttca accatatctt
    24721 ttccaacaag gcaaggaatt tcaaccattg catcatctgc aaaattttca atagctccat
    24781 tattaggaac aatcagtaaa aatctttcct gagtattaaa tgctaatgca caagctaatt
    24841 caacaataaa ttgagaatgt tcacttgcat caaaccatgt atctcttgct gtgccatttt
    24901 ttataattct ttcacattct tcgaatgtat ccttttctct tctatccata atttcatttg
    24961 ctcttgtata atttggatta gcatgttcaa cagtttcatc agccattaaa taatatttta
    25021 aataagtatt aggtaatgta gtcggttcta tttccaataa atcctttgct gctctcatag
    25081 tttctaacca actagattct ttgtgttgac cttgaggtgt aaattcctca tatccttttt
    25141 tagcacaata ttcctgtaat ttaggcatta aatcatttcc ttctttgtcc ttaatacttg
    25201 tccaccaccc gaaatgattc aatccataat atctaacatc aaagtcttta cgagaattta
    25261 aacctaaaac tctagcaaat atttcctcta aacatactgg catatcacaa atattaataa
    25321 ttcttgaatt tggtcttaat acacgacatg cttctgcaac tatagctgct ggatttgaat
    25381 aattaagcat ccaagcattt ggagaatatt tttccatata atcaatcatt tcgattattg
    25441 gacctattgt tcttaatcca taggcaattc cacctgcccc acaagtttct tgaccaacaa
    25501 ccccatattt tagtggaatt ttttcatctt gttcacgtaa agcatataaa ccaacacgaa
    25561 tttgtgccat tacaaaatca atatcagtaa atgcttcttc tggatttgtt gtataactaa
    25621 atttaatttc tggatttttc tctctaacaa ttattgcaca tgcttcagca acttttcttt
    25681 gtctctcatc attattatca tataatttaa ttgattcaat aggaaattta gcaacacttt
    25741 caattaacat tgttacaatt ccagcagtat aagtactacc cccaccagca attagtatcc
    25801 tctgtctttt tctttccatc tttccttctc ctttataata aattttcaaa ttcttctctt
    25861 acacttggta ctgaaagccc aataataatc tgaatagctt ttccattatg cactaaacca
    25921 tgtgcaccta ctgccttaaa atcttcttct ttagaaataa ttgagtcatc tttcaaggtt
    25981 aatctcaacc tcgtagcaca gtttgtaaca tctacaacat tttctgctcc accaactaat
    26041 tttatgtatt cagatgcttg ttgagaggct ttactcaatt tatttccttt ttcatcttta
    26101 gtttctaagt attcattttt agtatatagt tttgtttctt cactatcttc ctctctacca
    26161 ggagttttca tatcaaactt cttaattaag aaagtaaata caaagaacca gataaatgaa
    26221 aatataatcc ctacaacaac ttgaacaaca tataccttcc agtgatacat tccaagagga
    26281 agccaattta atacaaccca gttaataatt cctccaccaa aatcaccaac aactcctagg
    26341 gcaaacgaaa tcgtttgcaa gcatgcatct aaaaatgcat gtactaggaa taaaagtggt
    26401 gctacaaata aaaatgtaaa ttctaacggc tcagtaattc cagttaatac tgcagtaaga
    26461 gtagcaggaa tcattaaacc taatactttt ttcttttttt ctggctttgc agttacatag
    26521 aaagcaccaa atacacctaa tgaaccaaac accttcgata atccggataa tgaaaatccg
    26581 attggataca tttcctttag agtcttagct gaagtttgaa aatcctgaat atgagtagcc
    26641 caatatactg atgtaccacc tggaacaaca acactatcat aacaaattgg tgcatataca
    26701 aaatgatgta atccagttgg tattaaaatc ttttgtaaga atgcataaca ccaaactcct
    26761 atatttccac tatttataaa aaacgtttgc aaatgtctaa taacattttg aatatgaggc
    26821 caaataaaac aagttaataa tgcaactggt atcataacaa agaaacaaat tgcaactaca
    26881 agtgatgagc ctctaaataa tccaataaaa tctggcagtt ctttatcata aaatctatta
    26941 tgaatataaa tgacaatacc tgatattaac aaagcaccaa ccataccagt atctaatgtt
    27001 ttaataccag caatgcttac aagtccactt ccagcactta tttcagctgc gaaatcaact
    27061 ccaaagcttt ttccccaaaa acttaacata gaacctacaa aataattaaa agtcaaatac
    27121 gttgctaaag cctccataca agctcttgct tgttgctttt ttgcaagtgc tattggtaaa
    27181 gaaatagcaa ataataatgg caattgatta aataccatcc aagctccact agatattaca
    27241 ccccaaaatt ggtaccaaat attttctggt gtagcaagtg aacccataat tgactgattt
    27301 tgaaatacaa ttgagaatcc aaccattatc ccagcaaaag caaataatag tgttggtgta
    27361 aacattgccc ctccaaaacg ctgtattttc ttcatcatat aaattactcc cctcctcata
    27421 tttttagttt atctttggcc ttagatatct attattttaa caggttaata aatatataca
    27481 atctttaggg tacattcgga gcttattcac agtcaaataa gtttttgcac atttttatgt
    27541 tcaaattctc ataaaaaaaa agaatgacta caatttgcaa tcaatccctt ctgtaatcat
    27601 tctatgcctt ttataaatac tatatttaat aaataataac tcaatcaata taaaatataa
    27661 cattgtcaat ttataactag ggtgcatttg taaaaatgag atattagtcg ctgaaatata
    27721 aattgattca tcacttaaat cagtcaatgt attattttta aactctgtaa ttgaaattat
    27781 tttagttcct attaatttta atcttctagc aatattaagt gtattctcat tctcaccact
    27841 tagggatata ataatcataa catcattttt actgacaata ttgtaaaaag aatcatcaaa
    27901 tgttcttccc tcaaaatcat atattatttc ttgacatgat aagaacattc tctttatctg
    27961 ttttgcaacc acattttgaa tatcacctga accataagcg tatattgttc ttgattcata
    28021 aagaagttta cacaatgcat cataatcttt atttttttga tcttcaacat acttaattat
    28081 tgaattacat gcaatatcaa atgactgatt ttctatgaca tgaacctttt ctgattccca
    28141 gcttaataat actttaaact cactgaaacc ttttaaatct aattttttaa caaatcttgt
    28201 aatagttgca cgagacacat tacatttcat agcaagttca tttattgaca tcttactaca
    28261 atgtttttta tttgcagtta tatatttcca tattaataaa tctgtatcat tcaatttatt
    28321 ataatttaaa aaaatcattt cttctaatct cataaactat tacccccttt aattaaaaat
    28381 cgtcataaat attataccaa tattcaacat taaattacag cgtattctta tatttactat
    28441 ttagcccata aaattgacta taaaaaatac tgctataatt gatgtttaaa aaaatgtagt
    28501 tttaatttta ttcttttatc taataattaa tattttcaag ttctagtgct tttcttatac
    28561 aattatcatc tttaaaatca gcagtccttt tacaatcttt tactttaaat tctggaatag
    28621 ttttaaattc ctcatcacat attccattct tatttaaata catacaactt gccattctaa
    28681 ctattagacc actatttttt aaattaaaca atacagggtc tatacaccct ccatctccgc
    28741 cagtagtttc accaactaaa gtcgcaaatt tattttcttt acaaaacatt gcaaatgact
    28801 ctgatgatga atatattttt ctatcaacaa gtaagtatat attaccctta aattttgaat
    28861 catctgtaga ttttatagta taggaagtat cttcaaaatc attaaaatct tttataattt
    28921 ctttaggtgc attttccttt acacttgctg gtaaattttc tacaggattt aattttatat
    28981 ttcttgcatt tgtatatttt tttactattt cattattatt tctataaatt ctataaccat
    29041 ttattttgac atcatttttt atgagtttag atacgattcc ctgccaatac ctatcacttc
    29101 cacctaaatt acctcttata tcaataatta aagctttatg tttttctaaa gtgttaatat
    29161 aatctccaat tatttttaaa tcattattta tagagccatt ctttgatgac attgatggta
    29221 aatacatata tccaatctga cccttaataa catcctttaa aattaattct ttttttacaa
    29281 aaatatcatc tggcattttt attttaagat tcatagagtc atatctatcc acaacttttt
    29341 tatcattaag aaaatcatac caatcattat tagagtatga tttcttaaaa agttcatatc
    29401 ttttcttatt atctattact tctgtgtgtt tattatttaa atcagataat atagatgaca
    29461 gttcttctat aaattcttca tcattttcag tgttttttat tcgttttgta tattcatcct
    29521 tatttgctaa ccaatcaata ttattaagcc tcttatttac acctagatat ggatatcctg
    29581 tctttacaac attatacaca taattaaagt catccacctt ttcctcttca gacaactgat
    29641 tcttttgaga ataagtagaa ttacctaata atattataaa tgctaaaata attactaaaa
    29701 ataatacttt ctttttcatg taattctcat ccactttcag attttatatt ttaatacatt
    29761 gtatcattat tttattatca cctaaacagt taaaagaaat tcttgaagat atattccact
    29821 attattctct ttaaaaattt ttatttgtat ttt
//
LOCUS       DAMJYX010000039        29618 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJYX010000039 DAMJYX010000000
VERSION     DAMJYX010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.039"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(164..2830)
                     /locus_tag="PC479_001869"
     CDS             complement(164..2830)
                     /locus_tag="PC479_001869"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2804416.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2906..3168)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3464..4291)
                     /locus_tag="PC479_001870"
     CDS             complement(3464..4291)
                     /locus_tag="PC479_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2804417.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4339..5775)
                     /locus_tag="PC479_001871"
     CDS             complement(4339..5775)
                     /locus_tag="PC479_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2804418.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5862..6731)
                     /locus_tag="PC479_001872"
     CDS             complement(5862..6731)
                     /locus_tag="PC479_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2804419.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6889..7542)
                     /gene="phoU"
                     /locus_tag="PC479_001873"
     CDS             complement(6889..7542)
                     /gene="phoU"
                     /locus_tag="PC479_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2804420.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7570..8334)
                     /gene="pstB"
                     /locus_tag="PC479_001874"
     CDS             complement(7570..8334)
                     /gene="pstB"
                     /locus_tag="PC479_001874"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2804421.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8353..9177)
                     /gene="pstA"
                     /locus_tag="PC479_001875"
     CDS             complement(8353..9177)
                     /gene="pstA"
                     /locus_tag="PC479_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2804422.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9179..10096)
                     /gene="pstC"
                     /locus_tag="PC479_001876"
     CDS             complement(9179..10096)
                     /gene="pstC"
                     /locus_tag="PC479_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2804423.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10477..10923)
                     /locus_tag="PC479_001877"
     CDS             complement(10477..10923)
                     /locus_tag="PC479_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804424.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTYCKHQVS"
     gene            complement(11441..12121)
                     /locus_tag="PC479_001878"
     CDS             complement(11441..12121)
                     /locus_tag="PC479_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804425.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12171..14186)
                     /locus_tag="PC479_001879"
     CDS             complement(12171..14186)
                     /locus_tag="PC479_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2804426.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            complement(14258..14935)
                     /locus_tag="PC479_001880"
     CDS             complement(14258..14935)
                     /locus_tag="PC479_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804427.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(15515..15598)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(15831..16673)
                     /locus_tag="PC479_001881"
     CDS             complement(15831..16673)
                     /locus_tag="PC479_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2804428.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(16925..18709)
                     /gene="pepF"
                     /locus_tag="PC479_001882"
     CDS             complement(16925..18709)
                     /gene="pepF"
                     /locus_tag="PC479_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2804429.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(18775..19440)
                     /locus_tag="PC479_001883"
     CDS             complement(18775..19440)
                     /locus_tag="PC479_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2804430.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(19591..19752)
                     /locus_tag="PC479_001884"
     CDS             complement(19591..19752)
                     /locus_tag="PC479_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2804431.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(19830..20339)
                     /locus_tag="PC479_001885"
     CDS             complement(19830..20339)
                     /locus_tag="PC479_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2804432.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            20545..20763
                     /locus_tag="PC479_001886"
     CDS             20545..20763
                     /locus_tag="PC479_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2804433.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            20781..22727
                     /gene="feoB"
                     /locus_tag="PC479_001887"
     CDS             20781..22727
                     /gene="feoB"
                     /locus_tag="PC479_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2804434.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(22906..23961)
                     /locus_tag="PC479_001888"
     CDS             complement(22906..23961)
                     /locus_tag="PC479_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2804435.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(24009..24809)
                     /locus_tag="PC479_001889"
     CDS             complement(24009..24809)
                     /locus_tag="PC479_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2804436.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(24802..25593)
                     /locus_tag="PC479_001890"
     CDS             complement(24802..25593)
                     /locus_tag="PC479_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2804437.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(25633..26055)
                     /locus_tag="PC479_001891"
     CDS             complement(25633..26055)
                     /locus_tag="PC479_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2804438.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(26071..26550)
                     /locus_tag="PC479_001892"
     CDS             complement(26071..26550)
                     /locus_tag="PC479_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2804439.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(26682..29438)
                     /locus_tag="PC479_001893"
     CDS             complement(26682..29438)
                     /locus_tag="PC479_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2804440.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         9519 a         5231 c         3222 g        11646 t
ORIGIN      
        1 ttgcttataa attttaagac ataaaattca tatattcaca acataaaata aaaatataac
       61 acatctgtaa agaattataa catcatattt gtatatatat aaagaaaaac atacaaaaag
      121 tatagaatat atactttttg tatgttttta ctaattctta tatttatttt attttagatt
      181 ctaaatttgt taatctttcc tcaacagatt taatcatctc ttcaaatttt tctttttttg
      241 ctttttcttc ttcaattaaa ctttcaggag cttttgctaa gaaaccttga tttgcaagct
      301 ttccattaac tctctttatt tcgccttcta actttttctt ttctttagat aatctctcaa
      361 tttctttttc aaaatcaact aattcattaa gaggtataaa tatttcaaca ccatcaataa
      421 ctactccaac tgcatcttct ggaacattag atttatcttt tgctatctct acatttgaag
      481 cagatgctaa agttataaaa tagtctttac caagctcaat agcttctatt ttttcttcac
      541 ttgggattat tatcaattta gctttcttag aaggtggtac attcatttct gctctcacat
      601 ttcttatact tcttatacct tccatcaata agttcatcat atcttcttct tcagccatgt
      661 tatcttcttc attatatttt ggccattcag atactattat acatccttca acagtaggaa
      721 gatgagtata tatttcctct gttataaatg gcatgtatgg atgtaataat tttaatattt
      781 tttcaagaac ataagttaat gtatataatg ctgcagattt agcatttgca tcttctccat
      841 ataatctagg tttaaccatt tcaatatacc agtcacagta ttctgaccaa gtaaaatcgt
      901 atatcttttg aagtgctatt cctaaatcaa acttatccat gttattagtt gcttctttta
      961 caatattatt tgctcttgat attatccatt tatcagctag agttaagtcc tctttaacac
     1021 tttctctagt catgttttta attatatctt catcaatatt cataaataca aatcttgatg
     1081 cattccacaa cttatttgca aagtttctag caaattctac tctttccata tagaatctca
     1141 tatcatttcc tggggaattt ccagttgtta atgtaaatct tagggcatca gctccatatt
     1201 gctcgattat ctccaatggg tcgataccat ttcctaaaga tttactcatt tttctacctt
     1261 gagaatctct tactaatcca tgaaccaaaa catggtcaaa tggcttttca ttcatacaga
     1321 acatacctgc aaatgccatt ctaactaccc agaagaatat tatatcgtat cctgtaacaa
     1381 gtacacttgt tggataataa taatctaaag cttcagtctt atttggccat cctagagtag
     1441 agaaaggcca taatgcagaa ctgaaccaag tatctaaggc atcttcatct tgtttaaaat
     1501 gagtgcttcc acatttagga cacttatctg gcatttttct agcaactact atttctccac
     1561 actcttgaca ataataagct ggtatttgat gaccccacca taattgtctt gatatacacc
     1621 aatctcttat attttctagc cattgtaaat aagttttatc aaatttatct ggaacaaatt
     1681 ctaattctcc ttttttaaga atatctatag ctggttttgc aagttcttcc atcttaacaa
     1741 accattgctc tgataatcta ggctcaacta ctgtatggca tctataacaa gttcctacat
     1801 tatgattatg ctcttttatc ttaattaggt aaccttgttc ttttaagtct gctacgatag
     1861 cttttctaca ctcaaatcta tccatacctt catattttcc acaaacttca ttcatagttc
     1921 catcttcatt catagtattt aactgctcaa gattatgtct taatcctact tcaaaatcat
     1981 tagggtcatg tgctggagtc atcttaactg caccagttcc aaactctaaa tcaacataac
     2041 tatctgcaac tattggaagt tctcttccta ctaatggaag tattgctgtt tttcctatta
     2101 aatgtttata tctatcatct tctggattta ctgctatacc agtatctcct atcattgtct
     2161 caggtctagt tgtagctatt tctaaaaagt cttcactatc ttttagtggg tactttatat
     2221 gataaaagtt tccgtcgtgt tcttcatgtt caacttctgc atctgataaa gtagttttgc
     2281 aatctgggca ccagtttatt attctatttc ctctgtatat gtgacctttt tcatataggc
     2341 ttacaaaaaa ttctataact gcttcattac acccttcatc catagtaaat ctttctttat
     2401 cccaatcaca agaatcacct agttgtttaa gctgattaga tatttttcct ccaaattcat
     2461 ctttccattc ccaggctctc tttaaaaatt cttctctacc tatttcatac tttgtcttgc
     2521 cttcttgttt ttttattctt tcaacaactt taacttctgt tgctatagaa gcatggtcag
     2581 ttcctggaag ccaaaaagct tcatatccat ccatacgttt ccatctgatt aatatatctt
     2641 gaagtgtatg gtcaagagcg tgtcccatat gcaattgccc tgttatattt ggtggtggca
     2701 tcattattgt aaatggtttt ttatctggat ttggtttaga tttaaaatac ccttcatcca
     2761 tccatttttt atatagccta gcctcaaaat cttttgggtt ataagttttt gataaattcg
     2821 tattttccat tgtcctgcct cctgtatttt ataaataaat tttttaaata tctttagaaa
     2881 taaaaaagag ctcttcatcc tctttaaaaa ggacgaaaag ctcgcgttac caccttaatt
     2941 ccaaagctta aaatacatta acatatcatt aaattattgg ttaatgttat tttatcaact
     3001 ttgactctca aagaatttaa cggttctaac cgtctaaact tacttatagt tcagtttaga
     3061 aactccaaag ctacattcag taaatcttat acttagaaaa tcttacagcc agtgaatttt
     3121 cctctctttt agtagtattt acttactcct cttcttctca gtctttaata ttacatatta
     3181 ttatatacaa atattattct ctttgtcaat tatttttatt ttattttttc tgaattttct
     3241 taattatgtc tattctcttt tatatagttg ttatatttta aaatatgttt ttacagttat
     3301 tctgtcttat aatgtcaata attgcaagat tattttattt taattttaat gctttctcat
     3361 taattttata ttatccttac agtatacctc ttattcgtat tttttatatt atatgtttta
     3421 ttttataaat aatttacttt atcttttact atatttaaat ttattataag ttttcttgcc
     3481 agaaattttt aggtgttata ttttctgtaa ttggcaatat tgtatatcct ctctctttta
     3541 agattcttat aatattattt aaagcattta aactttgctc tttctcatga attaatacta
     3601 ctaattcatc tctttttctt ccataatata atatattact tactatctga cttgtagaag
     3661 acttccaatc ctgtgtatcc aaattccagt cccaaattaa ataatcattt gccataagtt
     3721 tattatatga accttctggc atatatggtt tacttccgta tggtaatctt attaattttg
     3781 aactctgacc agttatttct tgtagagtat tattgcatgt attaaattcc ccaactgtta
     3841 cttctgggct tttatataac ttatgtatat catgacttac actatgaaaa cctattgagt
     3901 tttcttcttc caagactctc cttacttctt ccttgtggtt tttcatattc tgattaagca
     3961 tgaaaaatgt cgctttgaca tcattttctt ttaaaatatc aagtagtgca tttgtatatt
     4021 ttgatggacc atcatctatt gttatgtatg ctactttttc agaagtagcc cctggacctt
     4081 ttgtttcttc ttgagatgct ttgtatatga tttctcctaa agtattactt tgagaaagca
     4141 gttccagtgt attttcacct tttgaactta tattttcaac tggaacatta ttaatttttt
     4201 cctccaataa tgttccagtt aaaattaaaa cagctataag aaatatcctt ataaatacat
     4261 gaaaaacagc cttattatct ttaattttca tcccccatat ctccctttta taaaagcata
     4321 agtttaagac ttaaaaagtt attttttata tttattctct ttcttattac tttttatatc
     4381 tctactaaat atttttttaa ccttttcttc ttctttttct cctaaagatt ctactctttt
     4441 gattcccaaa gaagttaaaa attgtaatac atttaaatta tcaaatggag tttccatatt
     4501 cgtactttca ctatattcat caacattctt agtaccttct tccacactta aaatacgctt
     4561 cggacctcct acacagcctc ctacacaccc cattccttct ataaatgtag cttctatttc
     4621 tttatttaga actttcttta gaccgtccat gcactctttt gttccttcaa atgccttttc
     4681 ttcaaatttt atatttttgt caattcttct aactgataac ttaactgctt tacttacacc
     4741 tcctgttcta gcataaactc tacctgaaag ggacgcctct actctattac tctcatccat
     4801 tattgctgga tttaactcta atgcatcaaa tatttcttgc aactctttaa atgtaagcac
     4861 aaaatcaact gcatctttta tatcttctaa cattgcctct ttcttttttg ccatacaagg
     4921 acctatgaat acaactttag catctggatt taaaattttt acagctctcc cacaagcaat
     4981 cataggagaa acggatggtg atacattctc caatatttga ggaaaactat tttgtatcaa
     5041 actaacccaa ataggacagc aacaactagt tataaagcat ccttcttcgt cctttttcat
     5101 atgctcataa taatcatatg cttcttttgc agtaagcata tctgctgcca aagcaacttc
     5161 tatcatatct tcaaatccta tcttttttaa tgcagttctt aatttaccag ggcttacatc
     5221 tttcccaaac tgtcctacaa aagctggtgc aactattgca tatacagggc tctttatatc
     5281 ttttaacaat tttatcattg gtataaattc tattttatct gaaattgcac ccaacttaca
     5341 tcttgataca caatcaccac aagcactaca tacatcttcc tcattacaat taatatgctt
     5401 acttcttatc ttacatgact catcttcctt attattttca tcacaatatt tgcaagcatc
     5461 tgatattaca gatattattg gtcttttgac ttcatctaaa tttacagctt catgtagtga
     5521 ctcttttaaa tttttatctg tatcttctgt cacatccaat cccatagtta ccctaattaa
     5581 atttaccata gtatctttat caaattttcc actaaaataa ttatttaaat tatcctttaa
     5641 attttcattt aaaattttat taggtaagtt ttcaatatcc tctatgtcat cattccacac
     5701 tgcttttaca agttcgccaa aaagcttcat tttatatatt tctagtattt tagaattgtc
     5761 atttctagta ttcataaaaa atagtacctc cataaattaa acttatatat gtttaatatt
     5821 tacaaagata ctatttttta tttattattt aactactatt attaatatga tttatttagt
     5881 tttctcaaca actctatttc aatcattatt ttcattagat gatagttgaa tacagggctt
     5941 ttttcttcat ctatattcca gtctagctta tccacattat atgttttctt aaggtattta
     6001 tatgtatgct ctttcaaata aagtttattt tcaagtaact ctatagactg catatgaaaa
     6061 tgtatttctc tacatatcct taaggcattc tcaatttttt ctatattagt ttcactcaaa
     6121 ctataatcca actcatcaat aagttttgaa tatatttctt ctaatttttc tacttcttct
     6181 tcaaatttct ctacatttaa attttctttt ctaagaattt tatcttcaat aagcaacatt
     6241 gaaagttctt ctatcttttt aaactcatct ataacactat ttagataatt tggtctagca
     6301 acataataat taacaagtac tccaaacaaa acacccacag atgtatctaa caccctatgt
     6361 aaagaataat acacagggtc actatttcct acacctagat gtatagccag aaaggttaca
     6421 caggcaacta ctactgactt tattttaaac agattgcata aatatattga gcacattact
     6481 cctagaccac ataaaaatgg gtctccagct ttaatgatgg caaatatgaa tcctattata
     6541 ccacctaaaa tagtaccttc cactctattg attcccgtct ttaaagaacc ttttactgta
     6601 tcttgaacag aaattataca tgctaccaga gaataaaaca tattctctac taaatagtca
     6661 ccaagaatca tacataaagt gacactaatt cctgttttta tagtcctcat acccatcttt
     6721 tgaagtttca aagttccacc tcctaaaaag caccttatat aaaatacgca tcctcatatt
     6781 taatatgttc ctagtatatc acaatttttt aaatttaccc attaatcata tttttttatc
     6841 aattctttct taatttagac aataaaaaaa ctgctttaaa gcagtttttt atcctatctc
     6901 aaccatttta ccctcaattg caaaaattat cttctcacat acatttgtta tatggtctcc
     6961 aattctttca aggtatcttc ctataagtag aagttttacc ccttggtcta tggtatttga
     7021 gttttcatgc attattttta aacaatcaat ttgtacttgt tcatacaatc catctacaat
     7081 atcatcttta gttccaacat cataagctat acttgcatca aataagatta aagcttctct
     7141 agtctgagac atcatagact tacattcttt tgccatctta ggaatatcta ttagctcttt
     7201 tatataagcc tctccaccta tcttcaacat ttctctagct atatttgctg cataatctcc
     7261 tattctttcc agctctatag atatattacc taatgaataa ataaatctta agtcccttgc
     7321 cattggttgc tttaatacta ttagctctat actcttatct cttatttctt ctcttaattt
     7381 atttatttct tcatcatctt ctactacttt tcttgcaccc tctaaatcct tctctatcat
     7441 acaagtcaca gacttttcta ttgcttcttc acatttttcc atcataagca ctgtttgttc
     7501 ttttaaagta tcaatactta tatctaagct attattgacc attgtctaac tcctttatag
     7561 ttatatattt taaccaaatc tacctgttat atagtcttct gttcttttat ctacaggagt
     7621 ggtaaacata gtttttgtat cgctaaattc tataacctct ccatttaaga aaaaagctgt
     7681 ttcatctgat atacgagcag cttgttgcat attatgtgta actatgacta ttgtgtagtc
     7741 tttttttaaa tcctctataa gttcctctac ttttaatgtt gaaattgggt ctaaagcaga
     7801 tgtaggttca tccataagaa taacttctgg tctcatagca attgctcttg ctatacatat
     7861 acgttgttgt tgaccaccag aaagtcctag tgcagatgat ttaagcctgt ctttaacctc
     7921 atcccaaata gctgcacctt ttaagctttc ttctacaatt ttatctaatt gcttcttatc
     7981 tcttagtcca tgtgtacgag gaccatatgc cacattatca tatatactca taggaaatgg
     8041 atttggcttt tgaaatacca ttcctacttt agtacgaaga tttataacat catctgaggt
     8101 atatatgtcc tctccatcaa cagaaatgtt tcctttaatt gttacatctt cgattaagtc
     8161 attcattcta tttaacgttc ttataaaagt tgattttccg catccagatg gacctattaa
     8221 agcagtaacc ttattctctt ttatatccat atttattttc tttaatgctt gcttatcacc
     8281 ataaaataaa tccaaatctt ttacactcat ttttatctta tcgattaatt ccattattca
     8341 cacctcttaa atttaataat tagctttatt taatttctta gcaacaaatc ttgctagcat
     8401 gtttagtgct aatacaatta ctattaaaac aattcctata ctggctgctg ttgctatatc
     8461 tccactctct ttagtcagta agtatgagtg tacagttaat gttcttgcac tatcaaatat
     8521 tccacctggc atcttggcta cagtacctgc tgtaagtaat attgcagctg attctccaat
     8581 tatacgacca actgataaga ttactccaga taaaattcct ggtattgcac ttggaagtac
     8641 taccttatat aaagtttgaa attttgtagc tcctaatgcc aatgatgatt ctctataagc
     8701 ttgaggaact gtttttaaag cttcttctgt tgtacgaatt attactggta atattattat
     8761 tgcaactgta agtgaacctg caactatgga atatcccatt tttaatgtaa ctacaaagaa
     8821 aattcctcca aataatccat atactataga tggtatccct gataaacttt cagttgcaaa
     8881 tcttatagct gtaactgctt ttcctttttt agcatattct tgtaaatata tagcagccaa
     8941 tattcctata ggagttgcta caactataga aactactact gtatataatg ttgtaattat
     9001 cattggaagt ataccaccat cacctgatgc tgaataattg ctaaataaga aattaaaatt
     9061 catgttgcca attcccttta taaatataaa tcccactata actactaatg atactattgt
     9121 aaatagtgct gatagatata ctaatgactt caatagattc tctttgaact ttctcatctt
     9181 aatttaccgc cttatttgaa agtttattta aaactaagtt taaaattaat ataaatgtaa
     9241 acaatactac tccagttgca aatagcattt cttgatgtgt accaaatgcg tatcccattt
     9301 caagtgctat attagttgta agtggtctta cactatctgt tattgcagtt ggcataactg
     9361 gtgagttgcc tgcaacaagt attactgcca ttgtttcacc taaagctctt cctactccta
     9421 acactactgc ggctaatact ccagattttg cagcaggtaa tacaactttg aatattgttt
     9481 ctatttttga tgcaccaagt gctaatgaac cttctttgta tgcttttggt actgctctta
     9541 ttgctgtttc tgatacagat attattgttg gtaacatcat tactgctaat actattacta
     9601 ttgctaacaa actttgacct tttggaagat taaatatact ttgtatagtt ggaactatca
     9661 ctgcaagacc aaatacacca tataatactg aaggtatacc tgctaaaagt tcaactgctg
     9721 gtgacattat ttttgctaat cttttaggtg ctacttctgc tataaataca gaggttaata
     9781 taccaacagg tactcctata acaagtgctc caagtgttgc aaatattgat gctactatca
     9841 tagatgatat tccaaattta ttggcagaag gaacccaatc tgcaccagtt aaaaagtcta
     9901 taaatgaata tccttcaaat ataaatggtc ttaatccttt ataaaataca aatcctatta
     9961 tcaaaagtaa acttgcaact gctactaaag cactaagtag aaaaatattt tttgctactt
    10021 tttcaattac gtattttgtc ttattgccat tattgtcatt tttttctaaa cctactaatc
    10081 tagattttac tatcataata ccctcccatt aaaatatact tatcgatttt cgttctgtat
    10141 tcaaaattta attttttata attcgtcttc atcaaatttc ctgtactttt attctacttt
    10201 ttttaagtta aatctatatt aggattaagt taagcaagtg taaaattata tttacttttt
    10261 aacattaatt ttacttttaa aactatattt gacatttact ttacaatata ctgttgtttc
    10321 tctatattaa aatagcagat atagataata atacagttta tgtataattt attacctata
    10381 tctgctatta agatttgtta actaaattta acttttatac tacatatata attattgtat
    10441 cctctagttt atatcactat tatttccatc aaattgttac gatacttgat gtttgcaata
    10501 ggtatgaata gccaaaaata taaaaatagg tatttctatc actgagaaaa acagtataat
    10561 gtgtggtgtc caaaaactca tacctcctaa gtgtaagaaa ttaaatgatg tcatttgata
    10621 taataaacta atcttaaaac caattccccc tggcggtaat aggtatccaa tccaactaac
    10681 acctattccc ataggaatta agcaaataga aaaagcaatc agcatagaag taagagaatc
    10741 cttacatttt gcagataaaa ataatgttaa actaaccatt gcaagtattg aaattagacc
    10801 accaatagct ataagaatct gtgcttgtag taaatttata tttactagac taatgacaga
    10861 aaacaacatt tgaaatgagg ttttcaaaca ttcagtacca aaagctaaat cagaaattgc
    10921 caaatagata gaaatacata taacaaacat tattataaat atagaaaata gagctaatat
    10981 tttagtaaac gctagacaaa tatgtccatg tttcgtacaa cgaaggatgc tatctgattg
    11041 tgtctgatac tcaccagaaa aagtaggtgc agcaattgca acatatataa ttaataatat
    11101 aaaaatataa aatattatat aatcgaaagc atcttctgga tttattaaga aattcttaat
    11161 cttattattt aactatttta ttatttaata tatattaaag gtaaaaaaat aatatataga
    11221 tttagatata caatttatct atcttttcaa atataatcta taaaataata gttccattac
    11281 gcattttttt atataataaa actgactgta atttaactgg caaagtctag aagaataggc
    11341 tttatcaatt aaattacagt cagctatttt ataataattt agtttagata ttgttagtat
    11401 ttcacattta taacaattat taatgatatt aatatattta ttattcttta ttaaaataat
    11461 atcctgctcc tcttattgtt ataattttct gaggattttt ataatctttc tctattttct
    11521 ttcttaatcc attcactgca actaccacac tatagttatc ctctaagtat tcattcatcc
    11581 atacatgttc atatatctct ttagtagtca aagtcttttc aggattctct ataaaaaagt
    11641 ttaacaactt attttctgtt ggagtcaatt ttattatttt atcttctaac tttattactc
    11701 cactagttct attatattcc tctaattttg tattattttc attattattt acatatgagt
    11761 tcattcttct taaaatagct tttacttttg cgtataatat ttctaaattt agaggcttta
    11821 ttaagtaatc gtctccacca gaatttaaag catttaaaat gctttggtct tcatttatat
    11881 aagtcatata aacaattgga caagtagtta catttctaat ttttttacaa agagtccatc
    11941 catcaccatc acttagaatt atttctaaaa atattaaatc atatttattg ctaaatattt
    12001 ttccaatagc ttcttcacaa ttatatgcta aatctatact tattcctttt tcctccagaa
    12061 atccttttag cttcttgcaa ttatccttgt ctccatctat tatcaatatc ttagtttgca
    12121 ttataatcac tcccaaatat cattattctt ttatattaaa taagctatta ttataaaaaa
    12181 tatagactta attttattgt aaaaacagtt ccttttccta gttctgaatc tacaaagatt
    12241 tctccaccat gctctcttat aatggatttg acaatatata gccctaaccc ttgccctttt
    12301 ggctcattat tgtctctttc tgtaaagaat ctgtcaaata cctttggtat attttctgga
    12361 ggtatacctt ttccattatc agaaatttta ataattgcaa atccatcttc tttatccatt
    12421 gataaaatta tctttccatc aaatggagta aaggtcaaag cattataaat taaattctca
    12481 aatgctcttg taagcatctc tttatttatc tttgcatata tttttttctc caatacgtaa
    12541 gtttcaaagt ttacaccaca tgcttgtgta tctggcaaat tattttcata tatctcttta
    12601 ataaaattat tcagacatat tttttcacta ataccaatgc ttttatcttg tgatgtaaat
    12661 tcttgcaaac tacgtactct attttgtact tcatttgact tttgatttat agcataaaga
    12721 taggttcttg tctcttcatc tacatgaata ttaccaacct ttatcaaatc tataaatgct
    12781 tgtatagccg aaacaggtgc ctttaaatca tgtgctacat ctgccaagaa tcttttacgt
    12841 tcatttaaca atgttgtaag ttcattagtt ctcacttcaa cttcatgttc aagattcctt
    12901 gtaagttttt cattctcttt tattattatt ctattcctgt ttatcatcat aacagaaaat
    12961 aaaataacaa ttacaaaccc acaatattct gtttgccacc ctgtaaatat aggctcaaat
    13021 ctattactcg taactgcatc aaataatatg ctaattccaa aaacagtgtt tactgaaact
    13081 aaaatataag aacttatatg ctcagagcat gtctttttat atattgaaac cagcgaagac
    13141 attaatatat atataaatac aatatattta tatatgtcta ttaacttccc atatatgttg
    13201 acaaagtaca tatcatatgg aagaagaata attggtatca ctatagatat gatactcatt
    13261 gtaattccta atggtaaaat taaaaatctg tacactttat tttcttctat tttggatata
    13321 cttccattta tacaaataat acataaaact accataaaat atgacaaatc ttcaaccgca
    13381 tatgtaaagc ttaccaaagg cagtcctaac aaatgtacaa agggatagga tacatggatg
    13441 gcaaagaaaa cacacataca tccaaaatat aagtaaattt tacttttgtt tgaaagaaac
    13501 catacagaca atgaaaatat agctattgca aaagttgtga agcaaattat actataaaaa
    13561 agtaatcgat aaaacatcat attacttatg tcctttgttt ctcctaatgc aggcggataa
    13621 tacaaaccac tataataatg tgaaaaattc gatacattga ttataatttc tgtatttttc
    13681 tttgcaataa aacttactac tgaattttta attttgggtc tatatttttc tgttcctaca
    13741 tctcctaatt tagaaaccat ttctccattt atccatactt cacttgcgga aaaaatctca
    13801 ggaagctcta aacttagtag cttatccttg ccttcatttt ttattctcat acgataagta
    13861 gcattcccat atggtgactg agtccccttt ctcatagaaa aatctggata ttgacctata
    13921 aaaacatatt ctggttttat atcttgctct gtaaaatcat tcggtttaaa tattttatga
    13981 ttataaaact cccagtcatc tatcaaaaag acaagtttac ctttagtgaa agtatcatta
    14041 tctatagtta aaattccatt acttgctcta atagacttat atgtatattt attatcatat
    14101 ttatacagaa gatttaatcc tgttattgcc aacagagaga ctatcaatac aatgagagtt
    14161 ccttttagta tattatactt tttcattgta tcaaccacat ctctttcata gtagtattaa
    14221 ataaaacaag aactatttat taattacagt atctcactta tggtacaaac ttatatcctt
    14281 ttccccatac ggtttgaatg tattgatgct ccctgcttaa agaattaatt ttttttctca
    14341 tctgtgcaat atgaacctgc actgtatgtt gatctccaat atcaggcatt ttccaaactt
    14401 ggttatatat ctctatcttc gaaaatactt gttctttgtt ttcagctaga aacaataata
    14461 tttcaaattc catagtagtt aaatctatac tttctccatc aaaagttact tttcttgaat
    14521 ttacatttat tactaacgct gaaaacttta acacattagg aactgatgta atatttttgt
    14581 actgcctcat acgagcatat attctaagtt caagttcttt taaactataa ggttttgtta
    14641 tataatcatc ccccccagct aaaaatcccc gaactttttc ttcttcatgc tcataattag
    14701 ataaaaatat tataggtatg ttaacctttt cttttatatc attgcacatt ttatatccat
    14761 ctatatctgg caatgaaata tccaaaatta tacagtctaa aactatactc tctactatat
    14821 ccatagcatc tttagcagtc tctgatgtat atacattaaa acctatatta gaaaaataaa
    14881 tttgatttat gtttaaaata tcaacatcat catctacaac cagtaaatta tacattacac
    14941 cactccattc aaaggtattt taattataat tccaagtttt atagttatta caaattataa
    15001 tgttttattt tcatatataa ctggtattat tattttataa tatctttaca aactagacta
    15061 taatacttaa gtatttttga ataattcttg aatattgtat taaacattaa gattcattat
    15121 taaatactaa ttaaagtctc cgattgttgg agaatggagc ttaaagtcat tttcacgtat
    15181 aaatttatta tttttctaga aacttgtcta ttggctagtt tgagtattga tttatatgat
    15241 ttatatggtt ataatataaa ataaattgta ttattttggt aaatatattg ttacaaaagg
    15301 tttatattct gcatctcaat gtttataaaa actttatttt cttattataa tactattatt
    15361 cttttaaaaa aatttccctt ttccaacaaa tggctacttt aaaagccaaa attttaccta
    15421 tcaataaaaa tcttaacgac taatactctt attttcaaat tttgcaacat atttgttaat
    15481 tttaaaacaa aaaaagacta tgcaatataa aaattatatt catagccggt tgcaccacta
    15541 actccatata tctctagatg cccaataata agatatactt ctcagtttct aaaatatttt
    15601 tattaaaaat cttttatata ttttcataaa tttctttttt attctaacat tgtaatatta
    15661 ttttgtcaat atttccatat tcttagagat aagatactaa aatacctatt ttatctaaag
    15721 atatttatat ttctacattc aataatagac aataccctgc aagttaaagc atcattttta
    15781 tgagcttatc tcacagggta ttatatgttt actttatatt ataactcttg ttattgatta
    15841 actttaataa atcctttttc tgaaacaagt gcttgtcctt catcacttaa tatataatct
    15901 attaaagctt tacctttttc tgacaaacca tcttctttat taactaatat aaatggtctt
    15961 gatattggat atttattagc tattacattt gcatcagttg cttcaacatc atttattttt
    16021 aaactattta ctttctcatc aacatatcca aatgatatat atcctatagc attttcattt
    16081 ccttcaactg tagtttttat atttcctgaa ccatcactta tttgtgcatc tttagttagt
    16141 tcttctgaat caaaccctac aatttcttgg aatccatctc tagttcctga accttcttct
    16201 cttgatacta ttactattgg tgcatcattt cctcctactt ctttccagtt agtaatcttt
    16261 cctgtatata tacctttaac ttggtccatt gtcagatttt ttactgagtt tgcagtatta
    16321 gttatcaatg ctataccatc tattgctatt ttagtttcct ttaatccagc acttttttct
    16381 tcatctttta aatctcttga actcattcct atttcagatg ttccttctat agcatttttt
    16441 atacctccag aagaaccaat ctgttgtact tctacactaa caccttgatt tttttcttga
    16501 aacttttctc cgatagtttc cataagagga cctattgaag ttgaacctga aacagaaact
    16561 ttgcttgaat ctcctgaatc acctgaacct cctgagttag aactacatcc tactgctaaa
    16621 gctccaacta acattgtact agctaaaaga acgcctattt tctttttaaa cattttactt
    16681 acccccgctt atattttaat tttttgttgt catttttcta tattttaata atacagattc
    16741 tttgttaaat gaatattaga gcagggttaa gtaaatgtaa atattgttaa atacatgtaa
    16801 aaacttaatt ttatatgatt tattcttaat tagattaact ttttaaagtt actttaggca
    16861 gatataaaca taatacaact catattatca gaagtcttca ttgaagctat caacctatca
    16921 catcctataa ttctttttct agtttattaa ctaattcagc aaatacattt aaggcttcct
    16981 ctatagattc ttttttcgtc atatctacac cagcagattt taattgttct agtgggtatt
    17041 ctgaccctcc actttttaag aagtttatat atctatctac tgcagtactt ccttctgaaa
    17101 gtatctgttg acttaaagca cttgcagcag aaaaccctgt ggcatactta tatacataga
    17161 agtttgaata aaaatgaggt attctcgccc attctattcc tatttgttta tctactattg
    17221 cagattttcc gtaatacttt tcatttaatt tgtagtatat attagtaaat tcatcagcag
    17281 taagtggttc tccactctct accctttgat gtacaatttt ttcaaattca gcaaacatag
    17341 tttgtctatg aactgtagtt ctaaattgtt ctaaataata atttaataag tatatagttt
    17401 catccttaga cttagaattt tctaataaat aatttataag aagtaattca tttaatgtag
    17461 atgcaacttc tgcaacgaat atcttatagt ccgaatataa aaatggttga cttgttcttg
    17521 aataatgact gtgaacagag tgccctaatt catgaattaa agtgaataga gaattaaggt
    17581 catttttata gctcattaat atataaggat gtgaatcata acttccccaa gaataagctc
    17641 ctcctttttt accttcatta tcatatacat caatccaccc ttcatcaaaa gcacttttta
    17701 tgaccttcaa gtattcttct cctaaaggtt taagtgcctc taatatgata tcctgtgctt
    17761 tatcatatgg tatatccatg tcaaatttat ctgtcaaagg aacatacaaa tcatacatgt
    17821 gtatttcatt caatcctaaa aatttctttt taagctcaac atatttattt agattaggaa
    17881 tacttttatc tatagctgat ataagattat tgtaaacatc taaacttaca tcatctgaaa
    17941 ataaagacgc ttctattgct gaattatact ttctagtttt tgcgtaaaat atctctgatt
    18001 ttattccacc ataaagcgtt gaggcaaatg tatttttata tttctcatat gttgaaaatt
    18061 caccttcaaa agcgtctttt ctaactctac tattcttact ttttataaat aaagagtagt
    18121 ttgaatgaga aagcttaacc ttattcccct cctcatcttc tatctcagga aattccatat
    18181 ctgcatatgc taacatatcg tatacatttt ctggaattgt cgataattct gatgtagctg
    18241 caagtatttc ttcctctcgt tcactcaaag tatgtggttt atctctcaat atatccttta
    18301 tatattttgc ataaaaggat agcttctcat cttttaagta ttcacttaac ttattctctt
    18361 ctatcgccag tatttctgga actatataag aagtagccac acctaaatca gtagataaca
    18421 tttctgtctt agtggctctt ccttgattat cattaattct agtatcttca tgttgtttca
    18481 tatgagtata tacatataaa ttttgaacaa tcctagatgc attttcagag atatttaata
    18541 ctttatataa gttctcctta ctctcagaaa gcttcccttt atattctttt acttcttgga
    18601 taagattttt tactttactt atgtcttttt caatttcctc tttactggag tacatcttat
    18661 ctatcttcca tttgtacttt tcttctattg tacttctatc agttcccata tcatacacct
    18721 ctattttaat ttgttttata ttttattcac tttacaaaat atattgtata tattttagtc
    18781 caaataatct acatttatta ctccttctat catatttatt tctttcataa cgtcgtgtaa
    18841 gtgtattctc ttagcataag atacaaaaaa attaaccttc cagtattctt cattacgaca
    18901 ttctatttcc atatttctta cgataagttg atttttttca aatttatcat ataaaaaagg
    18961 tatagaatcc aaattgctta attttaatat caatacattc ttttcttggc taaataaaaa
    19021 cgtattgatt atcctaagca caaataatgc tatcattaca aataatccac ctagaattgc
    19081 caattcataa taaccatatc caactgctat accaatacat gcagttgccc acatcccagc
    19141 agctgtagta agccctttta ctccaccttt attttttaaa attgctcctg cacctaaaaa
    19201 accaattcca gataatactt gagcaggcat tcttgctggg tcagcatttg tatacaaatt
    19261 ataatcaaag aataattgaa tcgaagttat tgaaacaata caagaaccta ttgcaattag
    19321 tatatgtgtc ctaaatcctg caaactgtct tattttttct ctctctaagc ctattattcc
    19381 tcctattatt agtgctaata gtaaccttat tgctatctcc ttacttccta tatcaatcat
    19441 aatataaaac cctcctgact taattacaac ttatgtccta cagataattt acacatcaga
    19501 aatactattt tatatcttaa tattattatt aatatatgta aaatatagcc atctgttaat
    19561 ttttgataca aaaaaaacca caactgtggg ctatttgctt aggtacatgt atttatttat
    19621 taatatatct attttcttac ttgattctac taactcttct tgaaatattt ctggatattc
    19681 tatgtatgta tttatctctt ccctaactgc ttcaatctca ttttttaacg cttctaattc
    19741 attgttcgcc ataataatca ccactattac aaatttgtat atttttatat acattttatc
    19801 aaacaatatt atattttgtc aatattatgt caataaaact tactataaaa atgctttaat
    19861 ttaggtattg tatacctgga tactaagcct acaaacatac cagttactaa tgatgccatt
    19921 aacataaatg gtaagtaccc tatagcaagt atattttcta tgattaaact tgcaactatt
    19981 aattgaccta tattatgccc tattgcacct ataatgctta tccctattag tgataaatct
    20041 tttattttac tcgctaaata cataaataaa agactcagat aagctcctcc tatactaaat
    20101 agtagatatg ataaagggcc tgcaaatatt gatgacaaaa ttaccctgat agtaacaatt
    20161 ataacagtat ctttgacacc taacaacatt aatgaaatta aagttataat attgctcaaa
    20221 cccaattttg cccctggaaa tataactata agaggatttg gtatatatgt ctctaataca
    20281 tataaagcta aactataccc tatcataaga cctaaaaatg tcatttttct agttttcatt
    20341 aagttaactc ctccaaattt tatctatcat cttacattat aatacaaaat atataaaaac
    20401 acctaattta gactaaaata tttttaatct atttagaata aatattagtt atttcaatgt
    20461 gcaattacct ctataaatct tgactttttg tatcatactt gttacaattt taatgataat
    20521 gaatttcatt aggaggtgtt aataatgact gtttacgatt taaaaatagg agaaaaagga
    20581 tttattaata gtatagatgg agatattaaa ttatctaaaa gattgcttgc acttggatgt
    20641 ataaatagta ctgaaataga agttaaaaag gttgccccac ttggagaccc aattataatt
    20701 agatttagag gctttgacct tgcgattaga aaatctgatg ctaaaaatat ttctttacaa
    20761 taatagagga ggagctaccc gtgattaatg tagcattatt aggaaaccca aatgtcggta
    20821 aaactactgt ttttaatcta cttacaggtt caaatcaata tgtaggtaac tggcctggtg
    20881 ttactataga aaaaaaagaa ggatttcttg gaaaagagat aaaggttgtc gatttacctg
    20941 gtatatatgc aatggatact ttctcaaatg aagaaaaggt ttctaaatca tatttagaaa
    21001 atgaagatgt tgatgtaata gtaaatgtag ttgatgcttc taatttatca agaaaccttt
    21061 acttaacaac tcaacttatg caatttaaca agcctatagt tatattgctt aatatgttag
    21121 acattgcaga atctaaagga gttaatatag atgccaaaaa attaagtgaa gaacttggag
    21181 taattgttgt tccaattata gcaaaaaaga aaaatggtgt tgataggata gaagaagaga
    21241 ttaagggagc tacgcaaaaa gcttttatat atcagaaaaa ctttggaagt gaaacagaaa
    21301 catataaaaa attagaatcc attttatcga gatgtacaaa aactacatat acacaaaaga
    21361 aatctatcag tgataagata gacaatatag tcttgaatcc tatattagca tatccgatat
    21421 ttattggtgt attgttcctc ttatttaaat ttacctttga ctgggtcgga ggaccactac
    21481 aagagggatt tgctagtctt atagaggctt atatttctgc acctgttagt gatatgttat
    21541 ctaactctag cccatggttt aaatcactta tagttgatgg aataattgga ggtgttggtg
    21601 gtactttacc attcttccct ttaatattta cattattctt tggtatatcg ttgtttgaag
    21661 atagtggata tatgtcaaga acagcatttt taatggataa agttatgaga aaggttggtc
    21721 tatctggtaa agcttttata cctatagtta tgggtatggg atgttcttct ccagctataa
    21781 tggcaactag aacattggaa agcgaaaaag atagaagagt tacagctctt attgcaccac
    21841 ttatgacttg tggagcaaag ctacctatat atgcactatt tgtagctata ttctttccac
    21901 ataatgctgc tttagtaact acatcactgt atttggtggg tatagttatg gcaattgtag
    21961 ttgcattatt cttaaataaa acagccttta aaacagaggc tgaaccattt atattagaat
    22021 taccagaata tagaatccct actatagatg cacttattaa aaatacttgg aataaatcaa
    22081 aaggtttctt gataagagtt gttactgtca tgtttgcaat gtcagtcgta atttggggac
    22141 tttcttcatt caatgttttt ggatatacag aagatataaa tgtaagcttc ttggctactc
    22201 taggtcatat tatatctcct atattcaaac ctcttggttt tgatgattgg agaacttcag
    22261 ttgctatttt aagtggtctt ggagcaaaag aaatcgtagt aaactcttta aatatactat
    22321 atggtaatct aactgttgtc ttaccaacaa tatttaatgg agttactgca tatacattct
    22381 tgatatttac tgctttatat actccatgta tagctgcact tgcaactatg aaaaaagaat
    22441 atggcaataa aatgatgttt acatcatttg catatcaatt tatactagca tggataatgg
    22501 cttttatagt taaaagtata ggtggagtac tatttatggg taattcaata atagaagctt
    22561 tgataggtgg aataattata gtccttgcac tctttattct gtttaataaa ttcaagtcta
    22621 caaaaaacgg aaatgtctgt agtggatgtt ctatttgttc tggatgtcca agtgctaatt
    22681 cttgctcaag taaggaagac agcaataaaa aaaataatgc acactaatat ataaatattt
    22741 tagtttaaaa taagataaaa gtgggtgtct caaaattaac tttttagttc atgaggcact
    22801 cttttttata gaaaatcaaa tatattttca tcatttcaac agtaaaaaag agcttatcta
    22861 gtaaaaaaga ggcttatctc tattttgaga caacctcttt ttttgttact tattcagttt
    22921 cttgctacct aacttcatgt ggaacctagt atcttttgga acacttgggt ctacacctct
    22981 taatgtaggt atttccccac atattagttg tataggtact ataaaattaa atgtttgata
    23041 gtaaggatta tctgtgatat caatctttaa atttttatca ttttctgtaa catctctacc
    23101 tatagcaaat acattttccg tccaaccaga aagtacatca atcatcttag ttactctagg
    23161 ttcttttcct gtatctagta taaatatagt agaatcacta tttatagcat tatatatacc
    23221 atgtataaac tcttcaaact catacccagt tacaggtatt ctcattgttt ccaataattt
    23281 taatgctgct tctaatgtat ctccatatat atcagagtga cctattattc ttatttcttt
    23341 agaattaact aacttttctt tgtttctttc aatccattgt ttagagagct tatacactgc
    23401 ttcaaatcta ttaattgcgt ctaatatctt atttatttcc tcattgtact tttcactaga
    23461 tattatacct ttttctctag ctatttgaag acctaataac attaagttta actttgtaca
    23521 ataatatcct tttgttttag ctccagattt ttcttcacca caatttactg ttaatatata
    23581 atcagaaatc tcatctatta aagcattctt acaacctgcc atagatgcta ttttacagcc
    23641 cttatcttca gctaacttca tagcgttgta tgtagaataa ctactcccac cttgagatac
    23701 acctactact agggtatttt cattatcaaa cttgaatgta tcttcagtta tcataaatgg
    23761 atacattttc acaacatcta tatctaataa attttgtaaa tatggttgta cttgcactcc
    23821 tgagtgatag ctagtacctg aacctgttat tataatcttt tttagatttg ttctcttaac
    23881 ttcattaaat aagctatctg cattagatat aatttctctc attctaactg gtgtttctaa
    23941 catataatct tgtattgtca ttttatacac cttccttatc tatatattca ataaattata
    24001 attaatttct ataacaaacc tatagcatgt aaagcgattc ctgttactat agtgcctata
    24061 gttaattttg ttacacttac ctttctcttt aacatgtagt atattacaaa tgcaaaggtt
    24121 aaaggtaaca tattaggtat tattttatct agtatatctt gtaaaattac ttctgcacca
    24181 tttaagttga atttcagagg agtagttata ctcaacatag ttgcaaccat tcctcctaca
    24241 acgaataaac caacagttgt agccatagac attattttct ccataagtcc ctctctttgt
    24301 attctttcaa gagaatttac ccctacttga taaccatact taagtccaaa tattctaagg
    24361 gcaaatgctg gtatattgta taatagtaag aataatagtg gtccaaagat attaccttct
    24421 tttgcaagtg atactcctac tcctgctgct ataatcctaa atgttcccca gaataatgaa
    24481 tcaccaattc ctgcaagagg tcccataaga gctgttttaa cagcactaat tgaactttca
    24541 tcaaattctg gattattagc attctcttct tccatagctg ctgttatacc taatatagca
    24601 ggaactacta ctgtcgttgt attaaaaatc tccaaatgcc tttctattgc ttttgcttga
    24661 ttttctttct tattgtatag cttctttatt gcaggtaaca ttgaataaca gtaacctaag
    24721 ttttgcattc tttcgtaatt aaatgcacct tgaagagcaa atgaacgcca aaacatactt
    24781 cttaattctt tttttgttaa tttattcaaa gtcaatctct ccttccgtta aagtattgat
    24841 tgctaattga ggattttgat tattttcttt gttattgata taaaagttta gtacaaatgc
    24901 tacacatact ccaaataaag ctatagcagt tatatctaat ccaccataag atgctaacaa
    24961 aaaccctata aagaaaaata cagacatctt ttttgaaagt aacatatcca taagcaatgc
    25021 aaaacctact gctggcaaca atgttcctgc aacacccagt ccgtctaata taactttagg
    25081 tattgcattt atcaatgctg taactttatc agcaccaatc ataattgcta aaaaggttgg
    25141 aactgatgta cttaaaaaga ataatattac tggcaaccac atcattatgg taactttctt
    25201 aaaatcagct tgtttagcat agaaaactgc cttttgagaa aagtacgaat ttattattct
    25261 aactaatact ccaagagatt gtgctaagac tgctactggt actgctactg ctaaagcaac
    25321 ttctgcacca ttacctgata atatagcaaa agctgtacct aatacacctc ctgttactac
    25381 atctggagga gtagctgcac ctattcctgc aattcccatc caaattaatt caagctgtgc
    25441 accaatcata attcctgatt ttatgtctcc taaaactaat cctactaaca tacctgtaac
    25501 tattggccta tcaagcatag attgaccaaa tatacgccca tctagtatac caactcctgc
    25561 tataatagct aacatagtag cttgtactaa catattgaac ctccatttta acttagttta
    25621 atttacatta aattacatta catttttgat tttgttttga ttttctaata actcatttac
    25681 ataaacaatt ccattttttg cttcctctaa tcctgattta actattagtt ttgtatcctc
    25741 ctcaactaat aaaagtatag ataaaagcat tggtaaattt actcctgtca ataattcaaa
    25801 tttgaaccct tgtaaaagta agtttgtaca tatgtttgcc acacttccac caaaaacatc
    25861 tgttaagaca acaaattctt cactatcttt attatttgat aatatacttc taatttcttt
    25921 ctcagcatca tctgctgatt tatccgttgt catacaaaaa tactcaatat tagttaatga
    25981 accaattatt aatttacttg tgtcaacaag ctctctggac aattctccat gtgttgcaat
    26041 tagaaatttt ttcattctct ttctcctaaa ttattttatt aaatctgata gttgtttctt
    26101 tttgtctcta ggtaacattc taaattctat atcaatccct aaatcaccta tttgtttaaa
    26161 gttatttata tcatcatcat caacagcaac taaatctgta agtttcttct tcccttcctc
    26221 atatcttaat cctcctacat ttatttcttt taatactcct cctgaatttt tagctacaac
    26281 taaagcatct ttggtatttt ttactaacac taatacattg cttttctttg aagaagcaaa
    26341 tttttctact atttctccag attcctctac cccccttata tataaagtta cattgctagg
    26401 tttcgctaag ttcagtgaca tagctttcat ttggtcattt tttgcttcat catttgcaac
    26461 tagtattaca tttataccta gtgatgttgc ccatccatat gcaacctgac catgtatcaa
    26521 tctgtcatca attctcatca aagataccat tttccccaaa tcctccatca tacattaaaa
    26581 tttttattgt ttatactctc atttatctat aaaagcaaaa agtatgccaa atactggcat
    26641 acttttttaa atttcattca cttattttgt aatcttgtat tttactgtgg tagaccatag
    26701 ataatattat aaatatatcc tatctctgaa attggtattt gcacactata actcttttct
    26761 agcacactaa atgcattttt tattagtttt acttcactat gatggttttt gattaacaaa
    26821 tccatatcag tatactcttc aatttcagtt tgtctaacta atctttcaat catacaactt
    26881 aagtgaacat ataaagaaat ctttttatca tttgaaagtt ttttcttttg aatattttct
    26941 atagcattaa ttccttcttc tattctctct attatctttt ttgaatctaa aatagttaga
    27001 ctatctatta ctctaattaa tgaaaaattc tttacaatag aatcatttat gttctttatt
    27061 ttttcatttt ctactactct gctaaatatt ctaaaaacat catcttctcc ttttcctgat
    27121 attatatcct ctaataatat aaaatctacc ccattcacat ttggattggc agttcctact
    27181 atagccatca catcatacat ttcaaaaact ggttctttat ctcccataga aacaagacta
    27241 tcataatcat aaggtattat tttgacatca actattccaa ttaaactttc ggaaagtaga
    27301 tgttggattt ttatagcagt acctatccct gtaaaacaag atgatattat agcatattct
    27361 ttattttttt gaggataata caattgtata ttaggtttag tttcttcatc cataatttct
    27421 gcaatctctt caatgttttt accttgcatt aataaatttc ctacttctag tgcagaaata
    27481 gtggatattt ggtctataaa taacattggg ctatttatct tattctttaa atcttcatat
    27541 ataagattaa gagagcccat atcaaataac acaattaaac cattctttat cctatctccc
    27601 tctaggtatc tagacatttg ttttgataca tccgcaatag tagcatctat aggcatatca
    27661 atagctgtaa atatattttt acccaacatc cgattacata cattcgccat actactggca
    27721 gtagcatatc catgtgctat aatagtcgca ttaacttgat taaatttcat tcttataatt
    27781 gttgatttaa taaatataat cattatgatt atatctacat tattaagata catatccaat
    27841 ctattcgtta ttaatgttga tattttactt attatagagt attctttgta actctctttt
    27901 tttaaatata ttagtaattt attttctaaa gctttatact catcagacaa ttgataagat
    27961 tcgtccttca aataaagata agacgaaaat gccaatacat tattcccatc atattttata
    28021 ttgttattaa cttctaaatt tctaaatata tcctgcatta tcgatattat aagctccata
    28081 agtacgttat tattttcatc attatttgaa aagactaaat tgtccattag tctaatcatt
    28141 agattagaac tctttttact aaatttttcc tttgttattt catttttttc tagtctatca
    28201 aagtaatcta aacattttat gaaactttga tagattatat ttcttttatt tcttccattt
    28261 gacatttcaa tactaaattg tttaggagaa aagataatgc ttttgctttt accactttgg
    28321 aattgaaata tatctacatt ttctatataa agatttctag gaaggtcttg aagagtaaca
    28381 aaaatatttc catttcttct ttgatttttt gcataagctc gtccacatgc atattttatt
    28441 acatttttac attcacctat attccccttg aattttatct ttaaaatcgc atttaatgta
    28501 atttgagtta tctctatagg caaaccaagt accttacttt cttcataaat aaaatgttct
    28561 acaatctttc ttttttcaat agtccctctt tcttctatat tgggaacata aatatttatg
    28621 ggtattcttc ttaaaaaggt ttgtaaaaat tcagatagac tttctgttgt agcaaatatt
    28681 aatcttaccc tagcttttct aattactcca ttctctccaa tcctagaaaa cgtacctgta
    28741 tccataaatg taaataactt ttcctgacct tcactattta atctatgtac ttcatctaaa
    28801 aataaaacac caccatctga ttgttctatt agacctgctc tagattcatt tgctccagta
    28861 tatgctcctt ttacatgccc aaataataga ctagacaata actccaaatt attataatac
    28921 tgcgcacagt ttaacacatt gtatggagaa tttttttcta atacattact gcttacacta
    28981 tatttgtata ttatcttggc taaaaaagtt tttcctactc cactttcacc tgtcagcatt
    29041 attgtaagtc cgccatttgg ataaagtaca gcggttttta tttgttcgat tacttctttt
    29101 aagctattat catatcctat gatactatta aacacagtct cttgctttaa taatttacac
    29161 tcatttttaa gttcatctaa attttcataa atactttttt ttgtaggtcc tactacatca
    29221 tttattttat ttttaggtaa aaaaataaca ggtctactgt ttatctttat aagattatca
    29281 gattttacta aatcatttaa taatctactt gattgagttc tacttaaatt aaactttttt
    29341 actatatctt ctgttattat gccactttct tttttaagtt catcaaagtt gttaaataat
    29401 acacattcat ttacaaaact taaaatttca tccctcatgt tcaacctcca atacaaatat
    29461 caataatact ataatattag tatatcattt atatatttat gaaaaagata attatattcc
    29521 tatcttgatt ctcttttgta agtttgtaaa atcaaacttt ttataagtat aaaatctaga
    29581 ttttttaaca taatctcaag gttcttatct ttccatat
//
LOCUS       DAMJYX010000040        29262 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJYX010000040 DAMJYX010000000
VERSION     DAMJYX010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.040"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            251..2893
                     /gene="adhE"
                     /locus_tag="PC479_001894"
                     /gene_synonym="adhC"
     CDS             251..2893
                     /gene="adhE"
                     /locus_tag="PC479_001894"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2804441.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3253..3594)
                     /locus_tag="PC479_001895"
     CDS             complement(3253..3594)
                     /locus_tag="PC479_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804442.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3621..4292)
                     /locus_tag="PC479_001896"
     CDS             complement(3621..4292)
                     /locus_tag="PC479_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804443.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4304..6880)
                     /locus_tag="PC479_001897"
     CDS             complement(4304..6880)
                     /locus_tag="PC479_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2804444.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7088..8023
                     /locus_tag="PC479_001898"
     CDS             7088..8023
                     /locus_tag="PC479_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2804445.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8042..8779
                     /locus_tag="PC479_001899"
     CDS             8042..8779
                     /locus_tag="PC479_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804446.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8964..14021)
                     /locus_tag="PC479_001900"
     CDS             complement(8964..14021)
                     /locus_tag="PC479_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2804447.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14046..14465)
                     /locus_tag="PC479_001901"
     CDS             complement(14046..14465)
                     /locus_tag="PC479_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804448.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14699..14950)
                     /locus_tag="PC479_001902"
     CDS             complement(14699..14950)
                     /locus_tag="PC479_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804449.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15039..15770
                     /locus_tag="PC479_001903"
     CDS             15039..15770
                     /locus_tag="PC479_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2804450.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15829..16740
                     /locus_tag="PC479_001904"
     CDS             15829..16740
                     /locus_tag="PC479_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2804451.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16791..17603
                     /locus_tag="PC479_001905"
     CDS             16791..17603
                     /locus_tag="PC479_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2804452.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17712..18404
                     /locus_tag="PC479_001906"
     CDS             17712..18404
                     /locus_tag="PC479_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2804453.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18491..19432
                     /locus_tag="PC479_001907"
     CDS             18491..19432
                     /locus_tag="PC479_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804454.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19469..20626
                     /locus_tag="PC479_001908"
     CDS             19469..20626
                     /locus_tag="PC479_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2804455.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20778..21338
                     /locus_tag="PC479_001909"
     CDS             20778..21338
                     /locus_tag="PC479_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2804456.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21366..21857
                     /locus_tag="PC479_001910"
     CDS             21366..21857
                     /locus_tag="PC479_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804457.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22086..22637
                     /locus_tag="PC479_001911"
     CDS             22086..22637
                     /locus_tag="PC479_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2804458.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22723..23655
                     /locus_tag="PC479_001912"
     CDS             22723..23655
                     /locus_tag="PC479_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2804459.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23710..24573
                     /locus_tag="PC479_001913"
     CDS             23710..24573
                     /locus_tag="PC479_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2804460.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24820..26682
                     /locus_tag="PC479_001914"
     CDS             24820..26682
                     /locus_tag="PC479_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2804461.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26685..28151
                     /locus_tag="PC479_001915"
     CDS             26685..28151
                     /locus_tag="PC479_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2804462.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            28175..29011
                     /locus_tag="PC479_001916"
     CDS             28175..29011
                     /locus_tag="PC479_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2804463.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10831 a         3501 c         4670 g        10260 t
ORIGIN      
        1 ttcaaatgaa atatatctca tttagataaa aaattttagt aaaagtaaaa aaaataatat
       61 atttgttaaa aaataaattg acaaaatatt cagaaaaaaa cttcttgacc taagagagta
      121 aaataaataa aatctaaggt atagagatga aatctataaa attattttaa gtataagtta
      181 aaaatataga tgatgcattt aagatttata aaaattagag atgcataatt attttaggga
      241 ggtttttagt atggagaaaa aagaaaaggt agtagaaaaa tcaaatgttg aagtatgtag
      301 tcctgaaatt gtcaacagtg ttgaaacatt aagaatgaga ttagaagaaa ttagattggc
      361 acaaaaagaa tttgcaacat ttacgcaaga acaggtagat aagattttct tagctgcttc
      421 aactgcagct aatcaacaaa gaatacctct agcaaaaatg gcagtggaag agactggtat
      481 gggtattgtt gaagataagg ttataaaaaa ccattttgcc tcagaatata tctataatgc
      541 ttataaagat actaaaactt gtggtgtcat agaaaaagat gaagcatttg gttttacaag
      601 aattgctgag ccagtaggtg ttttatcagc agttatacca acaactaatc ctacatcaac
      661 tgcaatattc aaatccctaa tagcactaaa aactagaaat ggtataattt tctctcctca
      721 tccaagagct aaaaactgta ctatagaagc agctagagtt gtacatgatg cagcagttaa
      781 ggcaggagca ccaaaaggac ttataggttg ggtagatgtt ccttcgattg aattaactaa
      841 tgttgtcatg gcagaggcag accttatatt agctactggt ggacctggaa tggttaaatc
      901 tgcatactct tcaggtaaac cagctgtcgg agttgggcct ggaaatgtac ctgcaataat
      961 agatgagagt gcagatataa aaatggctgt aagttcaatc ttagtatcaa aatcattcga
     1021 taatggtatg atatgtgctt ccgagcaagc tgtaatagta ccagaaaaaa tatatgaaga
     1081 agttaaaaaa gaatttaaat atcgtggagc acatttccta aataaagaag aaactgaaaa
     1141 agtaggaaaa gtagtaataa ttgatggttc attaaatgcc agaatagttg gacaaccagc
     1201 acatgtaata gccaaaatgg cggatgtaga agttccaaaa acagctagaa taataattgg
     1261 tgaagtagaa tctgttgaat taaatgaacc ttttgcacat gaaaaacttt ctcctgtact
     1321 tgcaatgtac aagtctaaaa gttttgaaga tgcagttgct aaagcagaaa aattagttgc
     1381 agatggaggc tatggtcata catcttcatt atatgcagac tctataaatc atccagagag
     1441 agtagaaaag tttgtaaatg ctatgaagac ttgtagagta ttagttaata ctccatcatc
     1501 acaaggtggt ataggtgatt tatataactt taaattggca ccgtctctta ctcttggttg
     1561 tggttcttgg ggtggaaact cagtatctga aaacgttgga gtaaaacatt tgcttaatat
     1621 caagacagta gctgaaagga gagagaatat gctttggttc agagcaccag aaaaggttta
     1681 tttcaaaaaa ggatgtttag gtgtagcagc aagagaattt aaggatgtta tggataaaaa
     1741 gaaagcattt atagtaacag attctttcct atataataat ggatatacta agaaattaac
     1801 tgatttatta gatgagatgg gaattaagca tactacattc tttgatgtag ctccagaccc
     1861 aacattagct tgtgcaagag aaggagctaa agctatggct gatttccaac cagatttaat
     1921 aatagctgta ggtggaggaa gtgcaatgga tgctggtaag ataatgtggg taatgtatga
     1981 gcatccagaa gtagatttcc aagacttagc tatgagattt atggatataa gaaaaagagt
     2041 atatgtattt cctaagatgg gtgaaaaagc gtattttgct gctatcccaa cttcagcagg
     2101 aactggttca gaagtaactc catttgcagt tataactgac caagattcag gtgtaaagta
     2161 cccattagca gattatgaat taatgccaaa catggctata attgatgctg atatgatgat
     2221 ggagatgcca ccaagattaa cagcagcatc aggtgttgat gctttaacac atgcattaga
     2281 agcatatgta tctatgttaa gaactgaacc agctgacggt ctagcactac aagcaggtaa
     2341 aataatattt gaatatttac ctcgtgcata taaaaatgga aagaatgata aagaagcaag
     2401 agaaaaaatg gcaatggcat ctactatggc aggaatgtct tttgcaaatg ccttcttagg
     2461 aatatgtcac tctttagcac ataaattagg tgcattccac catgttcaac acggtgttgc
     2521 aaatgcatta ttaataaatg aagtaatcaa gttcaattgt gcagaagctc caaataagat
     2581 gggtgcattc tcacaataca gatatcctga ttgcatacaa agatatgctg aatttgcttc
     2641 ttttgcaggt attaaaggaa gtacagacca agaaaaagta gataatctta taaaagcaat
     2701 agatgaacta aaggcaaaag taggtcttcc taagactata aaagaggctg gagtagaaga
     2761 atctaaattc ttagaaagat tggatgctat ggttgaacaa gcttttgatg accaatgtac
     2821 aggtgctaat cctagatacc cattgatgag tgaattaaaa gagatatatc taaaagttta
     2881 ttatggtaaa taatatctat tgtgcaacat aggttctatt gggtttgtat ataaattatt
     2941 agatttgaat ataaatagag ttttattaga gacacgtaga agtaactata aataatagat
     3001 tataatttaa attctaaatg gaaatgataa aaagtcagta atttaatggt aatttcgaat
     3061 atgtgtattt aatcaattaa ctttaaaatt agtcggaaaa tttttttatt acatataatt
     3121 ttaaagcata atatataaaa aactttatga aaaaattata aaaatattca gaaaattcta
     3181 ttgactaatt tatttatcat gatataataa gtttagaaaa gaaaaaaatt ggggcctatt
     3241 aaggccccct tactattttt tgatagattt atttaaaatt actttctcat tttgattttt
     3301 tatatttatt atagagtcag tataatccac ttcaaagttg gcaaaagcag agaataaatc
     3361 tatatctctt aacaaatgaa ttgtcataca ttgattgttc ttatttagcc ttagaatcaa
     3421 tgctcttaca aatggaaatt gtattctaga attaaataag ttagaaccat cagtatctat
     3481 atctaagcta cgataattaa tatctataaa tatatctaca tcttcatttt cttctcttat
     3541 tattctgtag tcaaaatcca agtttaaatt taaattacct aaaatcaata acatttaaat
     3601 cctcttttct ataaatagtt ttagagtgtt aatttaagct ctccatctat tatagatata
     3661 attctatctg cttttctagc aatattttca ttatgtgtta ttattataag tgtttggtta
     3721 tattttttta tagaactttt tagtaaatcc atgacttctt ctgtagtttt actgtccaaa
     3781 tttcctgttg gttcatctgc taggattata cttggttttg cagataaagc acgtgcaata
     3841 gcaactcttt gctgttgacc accagataat tggttgggaa gatgattttt cctttcatta
     3901 agacccaata aatttataag gtcatcaata tattctttat ctattttttt attgtctaac
     3961 tttagtggta attctatatt ttcctttaca tttaatacag gaattaagtt atactgttga
     4021 aagacaaatc ctaccttttg tcttctatat cttgctaatt ctttatctga aagacaacat
     4081 atatctttac catctacctt tatagtacca ttacttggtc tttcaagacc acctactaaa
     4141 tgtagaaatg tacttttccc acttccactt ggtccaatta tagcaacaaa ttcgccttta
     4201 tttattttta aatttgcatt ctttagtgca tgtacagatg tttccttttt tccataaatc
     4261 ttacctaagt taatagtttc caatataggc ataattattc ctcctattct gtaatgttta
     4321 tagcttcaac tatactaatt ttatttatct ttcttgatgg aatatatgta gctagaatac
     4381 ctacaattat atttgctaca acaactagta tatatatttt gtaatctatt gaaaatccca
     4441 atccatatga tacaaagccc tgcatataat acattttaaa ttgtattata attcctgaca
     4501 cgatagtaat tatacttgag agtacaccat ataatattcc ttcataaagt atcatatttt
     4561 taaagcctct ctcagagata ccaatagctc ttaacattcc aaattcacta gttcttgaag
     4621 ttaagttata acttatgttg tttattatgt taagtacact gattatgaac attattgcta
     4681 ctataccata actataaatc atagttctag atgtcatctt atcagaattt tctttttcta
     4741 ccatcatatc tactgttgta gttccaggaa cttcacttcc aattttacct aaaaggttat
     4801 ttattttttt cttgtttgca ttttcattaa ggtctgcata aactaagtta tacttatcta
     4861 caccagttaa tttttttagg tattcgctac tcgttattac atctacacca tcatctccag
     4921 agtacatacc attatctgca aaaggataac ttataatagc tccaacttta aattcatatt
     4981 ctaagtattc ataattatct tttagacttt cataaagacc tgtatccatc ccaccctttg
     5041 gatatttgac ttttatagta tcacctactt ttatatctac agctggtgtt ccttcaccag
     5101 gagtaaaagt ctttgtgtat ttctgtgttt tagaaatttc tggtacatat acaatagcta
     5161 aattttcttt tttcattttt ttcaagttta tatttccact tactaagtat ttttctaatg
     5221 actttatcat ctcatcatta taacctttta gcttttgctt tagtgtatat ttgccagtag
     5281 tcttattttt tatgaacaga ggatttagct tataataagg agttgaattt atagcttcgt
     5341 agtaatctaa tctatttatt ttatttacct ctattcttgt gtttaagata ctagcagttt
     5401 tgactttgtc aattccatct atatttttta ttttatctat ctgattatca ttaaaccctt
     5461 ttatatcttt aaaagaagta ttgttaccaa tttcattgag ataaacattt gatagaataa
     5521 aatctgcatt catatatgtt tgttcttgag tagatttgtt ttgcacatct aaaaaagaat
     5581 atttataatt gtcttttatg acgattatac ctcctatact catagataat acaattataa
     5641 tgaacccctt tttatttcta aagatgtttt tcgcagatat agattttgtt actgacatat
     5701 ttaaagttaa ttttgatatt aaataattaa ctttggttac atttttatca gctccaaaag
     5761 tttttctgat agcatcaata ggagaaattc ttcttatttt taagtatgta aagaaactta
     5821 taattagtat tattagtatt gtacatgcaa ttgataagag aattatttta tctggaataa
     5881 caaaaggtgt aacatttata tttccttcaa attgaatatt tcctacagac ctattaaata
     5941 tttgtgcacc tcccatacct atacatatac ctataggcat tgctattaga gcaagtatga
     6001 aaagttcata aagcataagt ttgaaaatcc taaagttggt ggaaccaata gctcttaaga
     6061 cgccatattc ctgtactctt tggtatattg atataacata gatactatat attacaatac
     6121 cagcaaatag actcatagca attgcaacgt atttacttga attatctaaa gtaccgtttt
     6181 tcatagtaga ttctataagc attgagtttc ttcctatact tttctcatct aacatagcat
     6241 tttttattat attgtctatg tttgtattta tatcactgcc ttcattaaat tcaatataag
     6301 ttgtaaaatc attcagttct gattcattga gattagaaaa aatttcacat tgtccctttt
     6361 ttttctctat aaatctatct tcaaggattc caacaacttt aaaagtttct ggattttctt
     6421 tttgatataa tttaaaagta aggttttgat ttatctctgg ttttaagccc atactattta
     6481 aaatccattt ctcgactacc acctcattgc tagcttttgg aaatcgacct tttataagtt
     6541 tagaatgact agtcaaataa tttttatttg tatatagtat gtttataggt aatttttcat
     6601 taacatctgt agacgcatag taagatgtta tacccagatt tttaatatct ttgctagatt
     6661 ttatggactt taattgattt ttctttatat ttttaaagta tacatggtca gttccaagct
     6721 ctcttttcaa tctatctaca tctgcttgct gagcacttct tgagagagta ccaataccaa
     6781 caacgagtga tgtagcaaga attatactca caacaattgc taaagaccta gctttataac
     6841 tttttatata ccttagtact attttcaaat tatctttcat atttaccact tcctttctta
     6901 actaaatcaa ttatatattt atctaaaata tatagcaaag taagtatttt caaattttaa
     6961 gtagttaatt gtacaaattc atctgtattt ttacaagttt aattaatata tatgtaaatt
     7021 ttaacttttt acataatttt tttaaaatga tataatccct atataaattg tagaggggag
     7081 gggaatcatg gataacaatt ttgtatttcc tatagtaata ctaactttgt ttatatttag
     7141 caatcttata atgaataagt ataaatttta caaagaatta gacatattaa aaagaggcat
     7201 attaacattt tttatatcgt gttttgcatt gattttattt atggagggca ctaaatatat
     7261 acaattaagg ttaggatata gttttttact tgaaggagta ttaaatatta tacttttttt
     7321 ggttttaatt attttaagtt tttatacagg ctttaaagtt ataagattga taaataataa
     7381 taatgaacag gaatataaat tacaaaaact agatagtaaa ataaatcata aggataatat
     7441 aattatcaaa ttaagaagtc aaaaacatga ctatttgaaa catttgcaag tagtgtattc
     7501 tctattaaat actgggctag atgaagatgc caaagaatat ataatgaata tatcaaatag
     7561 ctttaaatca aaaaattcaa ggtatggaaa agtatcttat ttagatgcta tagcttcatt
     7621 aaaatacgag gaatgtcttg aaaaggatat atcttttgat gtctacattc aagattttat
     7681 ggataattta gagattgaac ctaatgatat gagttcagta ctattaaaca taatagataa
     7741 tgcaatagag tctttgtcta ctataaaaaa agaaaagaag tatataagat tacatgcata
     7801 tgaagatgaa tttcagtata ttatagctat aaaaaataat ggtcctaaaa tacataatgt
     7861 tgaaagtata ttttggaatg gatttagtac taaagaagga caagataggg gatatgggct
     7921 acatttagtt aaaaatatat tagataagta tgattatagt atacttgtaa atagtgatga
     7981 ttttttaact gaatttgtaa tcttaatccc aaaatattat tagttaggag gagaaaagtc
     8041 tatgataaaa gttttgattt gtgatgatga taagattgta agagatgata tagcaaatat
     8101 agctaaagag tctggatatg tagattatgt taaaaaagtt aaggatggta tagaggcaat
     8161 tgaatttata aaaaatgaga aaatagattt attgattatt gacattgata tgccatataa
     8221 aaatgggatt gattctgctg aagaaattct ttctatagat aaggatatac atatagtatt
     8281 tgtgacgggt ttcgctgatt atagtcttga aagctttaaa gtgcatccta tagattttgt
     8341 agttaaacca tttaaaaaag aaaaaatact tgattcaatt aacatagcaa tagaccatat
     8401 taactcccat aagattgcta ataataattt tatagaagac accctttttg tatataaaat
     8461 aaggaagcag atacatatga ttaattttga tgatatagtt atgtttgaga aaaattcaag
     8521 agcaattaat ctacacacta aagatgaaga tataataaag ttttatgaga actttgatga
     8581 tttaaaaaaa cgcctacctc ctaatttctt tatgacccat agacaataca tagtaaatct
     8641 aagatttata cataaggtta tacctataaa taaatcttca ttagagataa ggtttataaa
     8701 ttttcaagaa aatgccatat taaacaaatc tctagaaaaa gattttttat atagatttta
     8761 tagggttaaa agattctagt gaagatagaa gaaaaatact ttttgtgtga tataataagt
     8821 accgtaaaga taatgtataa gagaagttta tataaaaatt atttgtaatt atgatttaat
     8881 ctatataaat tacagtatag tatatattgg actttctaat ttttttaaga aagtccaata
     8941 tattttagag atagttttta tacttataaa ttacttaaga tactagggtc tttaatctta
     9001 ctatcttcag caagtaatag tatcttagac ataatctcag cagttttagg gtcttcatca
     9061 gcgaatggaa ggaatatctt acctctactt tgagaatgta taggaagtat attcaaagca
     9121 cccatccctt ttttatgtac aataccagaa cccatatgca cagaatactc acctaaatta
     9181 cctgctataa gagcatgaga gccttcaaaa ttgacattgt taatttttaa taataataaa
     9241 agttctttag caattgctat acgcatctct acagtagaat ggctagtttc agggtcaaca
     9301 ccaccagcat gagcgacact tacaacaagg tctatatcac gcataacttc tgagaataga
     9361 attggaggta tagaagctaa ttctaaactt ttataagttt ttctatcaaa aaattctact
     9421 gtttcaatag taggaggctc tatatctgct ggtgaaaacc aatctgcaag tgcaaacatc
     9481 tttgccacta tattttcttt ataaaatact ttctgtaatc cctcttcata atcgacagtc
     9541 caaagctgat ttctaagaag tgctacagtt ttctttggct gaatttggtg accagcatat
     9601 ctgcgagata tagttttttc agataattca tcactgttta ttctataata ctcacggaat
     9661 acttgcttaa atggttgaac aatttgctta ctaaataaat catgttggaa ctcactccac
     9721 ttaccagatt caaacaaatg aactggatgt gcaagtttta atttattttt tgccgtcaac
     9781 ttataagttt caccttctat attgcaaata aagaacttag acttatcatt ataaacatat
     9841 ccaatattat catcacatac ccatactaga tttttaatca aagggcagat aactggattt
     9901 ttatagatat tggcaatttc tttaactgta aatactattt catttttcat agcttgttct
     9961 aaactctgtt ttgcacgaga atactgactc tttaaatctt tttggacaga tttaagttca
    10021 agtacatatg gatgtttagc aatagctttt ggtatactct ttaaagactt gttgcctttt
    10081 tgaactttag tagatgcttt tccgttttca tcaacatcta tccaaattga agtaccatca
    10141 atatcttttg gtttaaatag atgagcaact tcatttagtt tttcagtctc aagagcccac
    10201 atcatacgat ttacatctcc aaatccagca cttctagcta gattttctag cgctatagta
    10261 caagcttttg attcactttc acgacgttgt gcaccaaatt gcttgctttc tttagcaaac
    10321 ttttgaataa attcataacg gaaagttgct tcttttaggt ctcctttttt cataggaagt
    10381 aaagagaaac ttctcaattt atcttgattt ctatttgttt caatttcttt catcaacatg
    10441 tctttatcaa gcttaccaag tgtagcatca gcatagagtt gtgcacgtct atgagagtta
    10501 ccaccactag ttatatattt agctgacttg taaagtatat tgaatctttc ttccccaata
    10561 gttttatatg aatctaaaaa ccaatctaca tcaaatgcac catcaatgaa actacgtggt
    10621 gaaatagggg agaatagagc aacctctgat tctttttgtg cagagaagcc ttcatttata
    10681 tgtgcatgga agtaccaaat tgtacttttc agacccttcc agtttaaata tttttcaatt
    10741 atgtttaccc attgaggggc atacatagca gattctatca atcttaactc agtaatgtcg
    10801 gtatttttaa gtaaactttt taattttttt gaatcatctt ctttagtagg atagcaattt
    10861 ttaagtagag aacttaaaac atcttttttt gaataattgc taccatatga atatcctctc
    10921 tggaaagttt ctttaccaag agcgaccaaa atatttacaa aataatttat accttcaaag
    10981 tattgtatat tattagctag attagacact tctgttggca tatcacctct acgtgattca
    11041 atttctataa ttcggtttgt aacagttttt aaattatctt taagccaagg gtacttttct
    11101 aatgtgtcgc cagcccatct aggatttgtc aatgttctta ttatatttga tgcgttgtat
    11161 ccacttatca tttctctatt aacttgctct tcagtcatta atcctaattc atgtgcacga
    11221 gcaaacacat ttatgctagt atagaaagga aacttatatt ctagcaactg ataatttgaa
    11281 aacgtagtgt agaaaaactt agtaaaagaa atatcatcat aaataaacga atgtgcttta
    11341 tcaagccaat agtttaaaat aggggcattt aaagcagggc agtattcatt ttgtctgtaa
    11401 tagtttttag taaattcaac tctttgtttt ttacattgtt ctatagtcaa taagtttcta
    11461 agtttatagt atatttctaa acaatagtta aatatttctt tatggtcaaa ctcatttagt
    11521 aaaactctta aaagttttct tgtaagtcca ttaaatttaa gtttttcaag ttttgtatat
    11581 attttatttt gttttttaga gtcaaggaca tttgaatata tagatgaaaa ccaacttttt
    11641 tctgtttcat tatatccaca tgtacttata ctataaaata agatatcaag tattaatttg
    11701 taatctaagt caagtttttt actagtttct ctccaaagct ctgctaatgg ataattatca
    11761 agagtatcat tagaaacttt atatttaata ggagtcaagt tataagtaga ataacctaaa
    11821 agtactttgt ctacattacc tgaccatctt tcaacttcat attcataatc tgcattttca
    11881 gtaataagtt catcaaattt ttcataaact tttattattt tatttatatc cgtatcgaga
    11941 attttttttg cttctttttt tgttgctaca gaaacagaat cctcacgagt agataaactt
    12001 atatttgaga taattttatt aagctttgat gagctattag aatcatcttt aaaatcaaat
    12061 tgttctggaa tttgaacttt gttttcaggg ttatatattc caaaaccatt ttctttgcag
    12121 taatcatttt ttgctttacc taacaaatta tcaattagta tttgaacttg agatgtattc
    12181 tttttagatt tatcaatatc ctctataagt aaaataagct tattatatag ttcttgtcta
    12241 gcagtattgt cttttaataa gtttacaatt tcaagacctc caaggcattt ttgagaattt
    12301 gaagatttta tcagtgaaga agcaatattt tccaaatttg aatcatcttg ttcaagtaaa
    12361 atttttatta ctgtttgtct aatagagctt gtctttaaag acaatagatt ggttatctta
    12421 tctaattcta catctgttag ctcaagcttt ctaagtagac tcaataccgt ttctctattg
    12481 gaaacactct tatcacttaa acattccaat aaaaattcac gttgtctatc tgtctttggt
    12541 tcatttacaa aattgactat aagtgaagct cttaaatctg aactcataag atgacaattt
    12601 tccataagca catctataaa ttgagcattc atatcgtatg ctgttaaagt catcatacat
    12661 ttaagtattt catgagaaga aatttcaatc caagtccatg gaaatacact tcctgaaaat
    12721 gattttgtct ttgaatttaa aatttctaaa gtatgtttta aactttcaaa ttgttctttt
    12781 ctagcatcaa agtctttgtc aataggagtg tttgacaagt caaaattagt ttctctgttt
    12841 tgccaactat agtagtagcc aacagaacct tctacataga aatttgtaat tacccatgct
    12901 agtatctcta aatcatcacc cttaatgttt ttagatgcta tagaatgttt aaaatatgta
    12961 ctttctgtat gcgataaaaa gtaaagagct acaagttttt tatatttttc ctgacaagaa
    13021 agtaagtttt caataagaga gtaagagtca tatatgttgt aagttgcagt agcccataag
    13081 gcaaaataaa tttcaagatt atcattgcta ttaattgatt ctatacggaa cttttcatca
    13141 gtcaatgatt tatatgaaag ttctaaacat ttacgtatta ttccaggttt ttggtctgaa
    13201 aaaccaagtc ctgtccatgt atccaaggct cttataacag aactaaatcg ctctaaattg
    13261 ttgtccaaaa tagtttttaa catgtatatt gatgcagaaa ttgtaccctc atctaaactc
    13321 tctacaattt gctgtctaag tccttcttgt tgtttggctg ccaaaagaag tttagaaagc
    13381 atttcatagc atttatcact atgactcttc atgataccaa ttatcatttg tcttgtaagt
    13441 agagcagttt gattttctcc aaatattact tcttctaaag cacttattac tttttgattt
    13501 cctttgtcta tttctagtgc tattttatca ctcattatag agtcatagta caagacctta
    13561 tttggaagag taagataatc tataactgaa aaatcataat aaattaaatc aacatagttt
    13621 gaaataaacc ttataatacc tctaaaataa tccacaagtt tatttgagcg atacccttta
    13681 cgataatatc ctcctgaata tgtataatcc atctttcttt taattaattg gcaaagttct
    13741 tctacttgtt cagaagtaaa tattaattct aatgcatttt ttaaaggtcc agaaaggagc
    13801 tcgtccatat ttttaatttc gtagtagtgg caattgtagt atttctttat catatttaag
    13861 aatttgtctt caggttctct ataatagtat gaactatttt ctatgcagtc ccaaagagac
    13921 tttgctaagt cgtagtactg ttttgaagaa gatttagtaa aattattttt aaaattttca
    13981 attattttag gatttttttc tactctagta cgttctaaca tttatgtacc tccaaattat
    14041 ttattttaaa acattcttcc agcaagcaag gtaagtctta taaaagtaaa tatatctcca
    14101 cttttcagat ttatagagga atcgagtttt tcaataacat cctcatttat aagcactttg
    14161 tagattccat cttcaaatgc ctgtaacgct acctctattg ccttttctac agattgttta
    14221 ttctcattgt aaatagagcc aaatccaact tttccaatat cagcttgaga ttcaatttct
    14281 tcattagtta agaagtttat tattgatttt tctgggattt tctcattata ttcaagaaca
    14341 tttttagata caatttcaga gatagcttct tttaggcaat tgatgttgtc agatacatca
    14401 aaaggggtaa gttctagcat aggtttgcgt ttgccaacag ccttaactct aaaataaatt
    14461 tgcataaaat cacctcgcaa aatattaatt atgtatttag aaatttaaaa atttatctat
    14521 ttgttatatt atatcatttt gtgaattatt aatgtgaatt atggaattat tttacaaaaa
    14581 atagaaaaaa agctatctta aagtctaaaa aagcaataaa agataacttt taactataat
    14641 tatatagaat tataactgta catttattaa attttaattt tagaattatt tttgataact
    14701 atcttttttt atttataaat tttgctccta aatatttttt gatatcttct tcaggagaaa
    14761 agtcatcaat aagtatttct ttcccagtgt cttcaaatac tacaagaact tcacagtcac
    14821 cttgtaagaa aaattttcta actgtatctt ctttaacttc tctaactttt attacattct
    14881 ttttttcttt ttcaaagtct tttgtgtaat aacttcccat catattaatt atttttgtat
    14941 atctattcat aaaatacctc catatgcgat taaatttaaa aaatatgtta taataattgt
    15001 ttgtattaac ttaataatta taacaaggag taataataat gactagtata aaaaataaag
    15061 atattttaaa ttgtatagat tattccatta aaaataagct ctttgacaaa ctaaatgatg
    15121 tatataagtc cttgccagta ggaaattgtt ctggatgtgg aaattgttgt atggagtcag
    15181 taggtataaa tttaattgag ttcttgaata tattttgtta tcttgaagat agagttgatt
    15241 taaaaaaaag ctgtataagt aaaatattag attattattt tgaggagtat acaagaaaga
    15301 acccatgtcc atttaaagat actaataata gatgtttaat atatgaagtt agaccattaa
    15361 attgtaggat gtttggacat tggaaaaaag aggattataa taaaaattta gataatgtta
    15421 ttgagaaaaa taataactat agagagttaa tgaaaaagca atatggattt gaaataaatg
    15481 atgaagtagt aaattacagg atagattatt gtgatagatt tattccaaat aaagattatt
    15541 taagtaaatc taagagatta agcttttttg atgagcttat gatattagac tcaaatattt
    15601 attcaagtgg aaatatagat atagacttta gggatagagg tatagttgaa tattttatag
    15661 agtcattact ttatagaaat ttagcatata atgtgaaaat aaggatatct aaagaataca
    15721 aaataagaaa aagaactata gatagaataa aaaggattgt tcttgtatag ttttatatta
    15781 gtaagtaata aattgtatca taaatattgt caaaatgagg tgaagaaggt gaatcttaca
    15841 aaagtaaaag gaaatacctt ttttataaaa ggtggcacga atacaggaat atatgtattt
    15901 gaagataaca cagcacttat gatagaccca gggttatgtg gttcaagacc taggagagta
    15961 aataaagtat tagatgaaaa taatatacag gttagatata ttataaatac tcatggtcat
    16021 gatgaccact atggagcttg taatcaattt aaagaatatt ataaagatgt gtgtataatg
    16081 tcatctgaat acgataaatt atatatagaa aatccagaat tgttttcaaa gtatattgtt
    16141 gggggaaaga gcaatgtatt tatggataat atcttaaaaa ataaaatgtt ggatgatata
    16201 aaaatagata aatctataga agctggaaaa attgaattaa acaatgaaat gtttgatata
    16261 attgaatttc agggtcatac acctggtagt atagctgttt tgacgaagga taatgtgatt
    16321 tttgttggcg atttattgat aggagatgaa attctatcaa aatacgattt cttgtttcta
    16381 tatgatatac aagaacaaat aaattcatta gaaaaattaa aaaatataga ttttgagtac
    16441 ttagttttag ggcatggcaa gcaagttata tcgaaagaag attcttataa attaatagaa
    16501 aaacatttga atgctttaaa aaaatattta tatcaaataa gagagttgct tgtagaaccg
    16561 aaatctttag aaattttatt aaaaaacatt ataaataaca ataatttaag caataattat
    16621 aaagagtatc atttttttaa atcttcgtta gtatctgtca taagttactt agttgattta
    16681 gaagaaatag ggtatatatt ggaaaatgga gaattgcttt actatacaaa aacaaaataa
    16741 attatgataa aataaagaag gtcataaaaa acaaataaag ggtgagcaat atggaatacg
    16801 aaaaatggaa tgagatacta gcaccttatg aacatgcagt agaggaattg aaaataaaat
    16861 ttaaaaatat aagaaaagaa tatttagcaa aaggtgaata ttctcccata gagtttgtca
    16921 caggtagaac aaaaaaaata tcttccataa tatctaagtt aaaaaggata aatgcaaaag
    16981 atatagaaac tgaaattgat gatattgctg gtataagaat aatgtgccag tttgttgagg
    17041 atatatatgc tatagttgat ttgataaaag taagaaatga tatgactata ataggagaaa
    17101 aagattatat tacaaattac aaagatagtg gttatagaag ctatcatgtt ataataaaat
    17161 accctataaa ctccattgcg ggttcaaagg aaattatatg tgaaatacaa ataagaactc
    17221 ttgcaatgaa tttctgggct acaatagaac attcattaaa atataaatat gaccattaca
    17281 tacctgagac tcttgctgaa aggcttagaa gagcatctga tgctgccttt cttttagacc
    17341 aagaaatgag tgaaataaga gaggacataa tgaaagctca ggctatgtac caaatgaagt
    17401 ctatagctat tagagatact ttaaatagaa tacaagagtt atatgacttg ggtgatacac
    17461 ataaagctat gcagtaccaa agaaaacttg atagaactga aacagataaa aatataactg
    17521 aaattttgga attaaaacaa gaaatagata tattattaga gcaatataag gatactaagg
    17581 tagatgataa agtagttcag taaaaactac ttgtagaaaa tttgctaaat attaatgggg
    17641 ctgcctctta atggaaaata atttctaaaa agagacagcc tatttagtgt aattaagaaa
    17701 ggaatgatat tatgagtttg tatgctatag gtgatttaca tttttcaaca tcagtcaata
    17761 agccaatgaa tatctttgga agtaattggg atggtcatga aaagaaaatt atagataatt
    17821 ggaaagaagt tgttaaggaa gaagatatgg tcttagtact aggagataca tcttggggaa
    17881 ttaatttatc cgaagctaaa aaagatttag atataataag caaacttcca ggtcagaaaa
    17941 tacttataaa gggaaatcat gattactggt ggacaaccgt tacaagttta aataagctat
    18001 atgaagacat gcgttttatt cagacaaatt tttatgaata taaagattat gcaatttgtg
    18061 gaggtagggg ctggatatgt cctaatgatg ttaaatttga tgagactgat gaaaaagttt
    18121 ataaaagaga agaacataga cttagactat cattagaatc tgctcgtaaa agtggtcact
    18181 caaaaataat tgtgattact cattatcctc ctacgaatga taaattggaa gaatctttat
    18241 ttacaaagtt atttgaagaa tataatgttg aaaaggtgat ttatggtcac ttgcatggta
    18301 aagaatcttt taaaatggga ttgaagggga ttcgaaatgg agtagaatat acattagcat
    18361 cttgtgacta tacagaattt aatttaataa aagttcacga ttaaaaaaca taaattggta
    18421 aatactataa gatacatgat taaaaataaa tatttgaggg atttcggttt tgagaaagga
    18481 gacacgactt atgaggtgga tattagtttt tatattttta tatcttattc ctttagtagt
    18541 gatattcaga aattacaaga attttaagag gtcttgtatc tacagtagta tttatgtggt
    18601 attagctaca acaataatga taagtaatat ttatatgagt ggacttaata aaataaaaga
    18661 gtctatgtat tatcacaatt atatttctga tgagacatat gatgaaaagt atgtatcaaa
    18721 ttttaaggaa aaagaaagta ctgatttaaa acaggataat aaaaagaatg aagaaatatc
    18781 aaataataaa aatttaaaca acaaacaaga tagaaatata caaaataaag aaagtaatga
    18841 agaaggaagt aatgatataa aagaaaagaa taattctgat agtaatgttt cagtgaataa
    18901 taaatcagaa aaacaagaag ataatacaaa agaaaataat aaatatacaa gtaaagaaaa
    18961 aaatagtgaa gatataaaaa aaatagatat ggaaagtata ggaattttta agaaagaaat
    19021 ttataatata gaaagagcag ctttagttcc aatgagagaa tgtatacctt acactaagga
    19081 tatagcaaaa aatataacta aattatctag tataaaaaaa gatgttacaa atgccagaaa
    19141 caagtgtaaa gaagttgtaa aagaatatga aaatatgcag attccagaac tttctaagga
    19201 gaagtataca gaaacattga gtaatgctag agatgacgta aagaaagcat atgagcttag
    19261 agaaaaatct atggaaagtg ctttgaaatt aataaatact aaaagcccta aatatatagg
    19321 gaagataaca gaatatcttg atttatcaga taagcagata gaaagtttta aaaatagatt
    19381 gaacgattta aaagaagaaa taaataattc agaaacaaat aatattaatt aataagattt
    19441 aaagatataa gttaaaaggg agattatgat gaataaacag agtataggaa aactaaatat
    19501 gttttttatg gggattactc aaagaaacct tgataatagg gattttttta ttgagttaga
    19561 ggtgattttt aaatctggaa gaaaagagtt taaaggaatt gcaatagagg aagataataa
    19621 atataaattt aattttaaag gaaaatcaga tttatatacg tttaatgagt tgcttaagaa
    19681 tatttctaaa gaggcagaaa attatgatgg attagtattt aaatatgttg agcgaggaac
    19741 tattgttgta attgaaggag acaataagaa ggtaaatgta aaatatttag ataataagga
    19801 agaagtacct aaaatagatg aatttacggc atctcaaata aaaaacagag attattatgt
    19861 aaaagtagga caagcaaatg cacttttaaa agaaattgga gtacttacaa aagatggtaa
    19921 gataaaaaat gataaaataa gaaaatacaa tcagatagac cattttgttg aattgataga
    19981 tagtatttta aaagaaataa aggataaaga ctgtataact atcttggatt gtgcttgtgg
    20041 aaaatcttac ttaagctttg tcttaaattt ttatataaaa gaagtattga agaaaaactg
    20101 ttattttata ggaatagatt attcagatgt agttatagaa gcatcaaaaa atatggctaa
    20161 aaacttgggt tataaaaata tgtcatttat aaaagaggat ttgactaatt atacacctaa
    20221 tagggatgtt gaccttgtaa taagtctaca tgcttgtgat acagctacag atatggcaat
    20281 aggtctagga attagagcaa aatctgaagc tatagtagta gtaccatgtt gtcataagga
    20341 gttattaggg caatatagat atgaagctat ggaaccaata ttaaaacatg gtgtatttaa
    20401 agctagattt gcagatttaa taactgatgg tttaagaaca ttgttattgg agggcaatgg
    20461 atatgatact tctgtagtag aatacatatc tccacttgat acaccaaaaa atttaatgat
    20521 aagagcgata aaaactaaaa cgaataatga caaagcatta aaagaatata aagaactgaa
    20581 atcacaattt ggagtggaac caaccttaga aaaacttata tattagttaa aaatgtaaaa
    20641 tttacgaaaa gcttgcttaa atatgcaagc tttttggtat tatataggta tagtcatatc
    20701 cagagttttg atttataatt aaagctgtgc tataatattt gattaatcaa atcaataaat
    20761 gttacatgga ggtaaaaatg aacaaattga atatatgtat tgatatagat ggaacaataa
    20821 caagccctta tcattttctt ccatacctaa atgatatctt taataaaaat ataactgaag
    20881 cagagtgtat aacacataat tgggaagaat tgtatggttc gggaataaga gatgtgtatg
    20941 cggagtttaa taataagtat atacactcat atgatgaagc aaagattgta gagggtgcta
    21001 cagaggtaat tagcacatta tctaaaggac ataatttatc ttttgtaacg gctagacatg
    21061 agtgtttaac tgatgtaact aaaaattggc tatcgagaca aggattttca gatatagatg
    21121 tttacttatt aggaagtgat tataaagttg aaaaggcaag agaactgagt tgtgacatat
    21181 ttatagaaga taatccttta aactcagtgc aactagctga tgatggtgtt aaagtaatac
    21241 ttctggatac taactataat aaagatgtta aacatcaaaa tatagttaga gttagtaatt
    21301 ggatagatat tgaaaagatt ataaaacaag gtatttaaag atttgtagat tgaaaggggg
    21361 agcatatgat agatttaaaa attgaaaata gaaaaggaaa aatccatgta aagagcagtg
    21421 aaataaaaga cattttagaa ttacgtccag acttcgaata tgtacaagat atatcaaata
    21481 ctataaatca agaaaatata ttggtatttg attgccagtt gacaggaaat tcttttgata
    21541 tggatgattt agatatagaa gaaatattgg aagagttagg tgaagaaata gatgagtctt
    21601 atttcagtgt tttatttgaa gatgtaagag cgtatttaaa agatgctact gatgagatag
    21661 aagcagactt acaggataat tacttatttg ataatttgag atgttatttt gatatataca
    21721 atataaatca agaatttaca gattttaagt ttgtattttt agtttctttt aaagatataa
    21781 aaatttcttc attagcaaac ctagctaaga ttgtttctaa gagacagctt ataggtgcat
    21841 ctaagtttta tagttaaaat gaaatattat tataataaaa gctagtaacc ttgtaaaaat
    21901 ataggttact agttttttat ttattcattt tcacaaaatt tcagaaaact atttacattc
    21961 taaaaaaagt aatgtatatt aaacataatg tacattataa tggataaaaa gtttctatta
    22021 aattactaag tttatttaat aatcaaaata atttatctta tattagtatg tattaggggg
    22081 tatttatgca aagtttaaat gaaataattg ctgaaaattt aaaaaagata aggaaagaaa
    22141 aacatcttag tttagacaag attgcacaac ttagtggtgt aagtaagagt atgctatctc
    22201 aaattgaaaa gtgtgaggta aatcctacta tatctacttt gaagaaaata actaatgggc
    22261 taaaaatttc atttacatca ttgatggaga ggcaggaaag tgacatagaa ttgattcaaa
    22321 aaagtgatat agaccatttt attgaagata atggaaagta tataagttat ccaatatttc
    22381 catttgactc caaaagaaga tttgagatat ttatgattga aatagaagaa ggtggaaatt
    22441 tagattcaaa tgctgaactt ccaggaacac aagagtttat aactgttttt agtggcgaag
    22501 taaccattaa aataaatgga gaagattaca ttgtatctag tggaaattct ataagattta
    22561 aagctgatgt atcacatata tatagaaact caggcaaagg tatagctaaa atgagtatgg
    22621 tagtatatta tatataaata atttaatgga attagagtga tatttagatg attttaggta
    22681 tgaagatata ttatatttaa gttataggag tgtgatttat ggatgaataa taaaaaagca
    22741 gtttttttct tttgcagtga tttaaagaaa gacccagtag ccagtaatgt gataaaatgt
    22801 tgtgaagaaa taatggattt aaatcaaact gatattgtta ttgatgataa ttttgtatta
    22861 gagtttaagg ataaaaacaa taacttattt cattttgtaa aaacaaaaga tgtaataagt
    22921 cataattata aatattatct tccaatttta aatagacatt ttagagatta tgattttgca
    22981 ggagttgtaa attggcatga gggagaaaat gctccagaac atatcctaac agctcataca
    23041 attggagatg ttccaacagg agaatttgga aattcaaatc caagatactt taaaaatcta
    23101 atcaatgcta ttgaggatat aaaaaatgaa aatctacttg atgaatttac tacattaaca
    23161 gaagcaactc actggtctgg aacaacatat ggtgaagaat ctaaattaat tacagaatat
    23221 tctgtaccta tgttggatat tgagatagga agtagtagtg atagttttaa taatagtata
    23281 gcaatacagg tattggcaaa atcattaatt agagtatttg attgtgatga acctttgaag
    23341 actttgttat gtgttggagg ggtacatttt gagaaatctt tctcagatat tataaaaaat
    23401 aaagaatata acatatcaat tggacatgta ttgccaaatc aatggattgt aagtggaatg
    23461 tatgatgatg aatctggatt tgagaaacta gaaaagtgta taaatagcat tgaaggagga
    23521 attgattgta tagtttttca tgataaatta aaaggtacct ataaagagca atgccgtaaa
    23581 ctaggtgaaa aacttaatgt acctgtattt aaacataaaa tattaaaaaa tccaaaagac
    23641 ttacctatat ggtagtctta tacagttaat ataacaataa gtcggattaa tttttaagcg
    23701 aggtcaaaaa tgatattatc ttattttgca ttattaatta gagtagtgct attagctttt
    23761 gaaagagtag ttgtaagatt attaggtgat gaagaaggag atatttataa aaatatagct
    23821 tcatcatttc tatttttctt tataggggga gtatgtttac ttccatttag cataatggat
    23881 agagttagtg attggtcgtt tttaatacca tgttatataa gtagtttagt ctattcaatt
    23941 ggttcagtag cgtatgtaac gtctttagca actggggaag tttcacttgt tactccaata
    24001 aatagtttaa attcattgtt tttactgctt ttatcagtta tatttttagg tgaaggttta
    24061 agtttagcaa aagttgcagg aatcataatt atgattggtg gagtgtttat attaaaaaaa
    24121 gtaagttctt ttttaaagaa tgtctctgta atttttaaca atttaccatg tagattaatg
    24181 tttttataca tatttttgca gtcagtagga agagtattgg ataaggcttt ttttgtacaa
    24241 gtatctccta tattatattc aactatactg tattttttcg taggtttaaa tttatttatc
    24301 tttttagcat ttaaaaagaa acaaaaaata atttgtgaga tttttactaa taaaaagggg
    24361 ctttcaattt taagtggtgc tattaatgga tattcttatt tagcattatt aattgcttta
    24421 aataatctag aacttagtat agcagagcca ttttctcaag tatctatgat aataacttta
    24481 atacttgctc attttatatt caaagaaaat ataaaagaaa aaataccagg ttcaattttg
    24541 atactaattg gtgggtggct actgttactt taattgacat aaaaaatgaa ttataaaggt
    24601 gctattagtt agtatcagaa aatttaaaaa agtattgaca attcttttag gcaaatgtta
    24661 atataataat acaaaatagt ataaatagaa gacatgttac tggtaatgca ggcaggatcg
    24721 aatatattgc tttgattttt caaggtgttt atataggttc tgcctttttt gcatcttgaa
    24781 tggccattca taaaaaatta tggagaagga gattacaaga tgaattataa aaaaactgcc
    24841 attgacatta ttgaacaagt tggtggttca aaaaatattt tagctttaga acactgttca
    24901 actagactac gctttaagtt gattgataga agtaaagtca atcaagatgc tttaaaaaaa
    24961 gtacaaggtg ttatgagtat agttaatggg gcacaatttc aagttgtcat tggaaatagt
    25021 gttgttgatg tgtatgatga aattttaaaa gtttgccctt tagatacaaa tgatagtact
    25081 gtaaaaactg aagaaaaaat aaacttagga acaagattta tagaatttat tattaatatt
    25141 tttcaaccac tgatttttgc tattgcagga gctggtattt taaaatcact gttgatgtta
    25201 ttagcaatgt ttaatatttt aaaatcagat tctactatat atacattatt agttgctatt
    25261 tcagatgcaa cattctattt cctacctctt atggtagctg taactactgc caatgtattg
    25321 aaatgtaatc gtttagtagc tattgccgct gtaggatact tattgcttcc agcaacaaca
    25381 actgctttaa gtgaaggtgt tgaactattt ggatttacaa ttccaaacat tgcctataat
    25441 gcacaggttt tcccggctat cttatgtgtg tctttcttgg caacaatgga gaaaattttt
    25501 aacaagtatt caccaaaacc aattcgaact ttctttgttc ctatgatgtc tttggcaatt
    25561 actgttccaa ttactctaac tattttaggt cctttaggat ataacgttgg tacaattttt
    25621 acaaccatta ttttattctt atataacaaa atgggattct tagttgtagg attacttgct
    25681 gcttttcttc catttatggt tgcaacagga atgcacaaag cactaatacc ttattcaatt
    25741 aatacaattg gaaagttggg ttatgaagct ttatatatga catcttcatt agcccacaat
    25801 atttctgaaa gtggtgcttg ttttgcagta gccttgcgaa caaaagatga aacattaaaa
    25861 caaacagcat tatctgcagg aatttctgcc ttgatgggaa ttacagagcc agctttatat
    25921 ggtattacct tacaacataa aagagcaatg ttaggtgttg tattctcaag tgcaatttct
    25981 ggtacctttt taggattatt tgcagtaaaa ggatttgtaa ttgttggacc agggcttgcc
    26041 agtatgagta tgtttattga cccaaacaat ggaaacaatt tgatttttgc gagtattggt
    26101 tttgttattg caatattagg ctcgtttatt attacactga ttatttggaa ggaagataca
    26161 aatacagtag agtcatcaat tggagatgtt gttgaagaag agctagaaga agaaaaagaa
    26221 gtaaatatct tagtaactcc agtagaagga aaagtaatag atttaagtaa ggttaatgat
    26281 gaactatttg caagtaagac tttgggagat ggagttgcaa ttataccaac caatggaaat
    26341 ttatacgcac cttgtgattc agaagttgtg atgttatttg aaacaaaaca tgcaattggt
    26401 ttaagaacaa aaaatggggc tgagattttg attcatattg gaataaatac tgtaagtatg
    26461 aatggagatg gatttaaaac atttgttaag acaggcgata atgtaaaaga aggagattta
    26521 ttaattcaat ttgatttaga caaaatctca catgctaatt tagattcaac tgttatgatt
    26581 gtaaacaata atggagcaga ttatgcttat aaagtgttaa accagtctta tggcaatgta
    26641 aaaaaaggaa gtatcttgtt tgatgtaaag ggaggaatat aaatatgaga aaatttccag
    26701 aaggatttct ttggggagga gctacagctg ctaatcaatt tgaaggtggt tggaatttag
    26761 gtgggaaagg gtggtctgta tcagatgtag caaaggcaca ctttgatgca gatgtgaaag
    26821 attataaatc taataatgaa atcacaacaa aagatataga agaaggatta gcacatcctg
    26881 aagatgaagt aaattatcca aaacgtcatg gtagtgattt ttatcatcac tataaagaag
    26941 atattgctct aatggcagaa atgggattta agacatatcg aatgagtatt gcttggtctc
    27001 gtatttttcc aaatggagat gataaagagc caaatgaaga aggtttacag ttttatgatg
    27061 atgtatttga tgagttgata agttatggaa ttgaaccact tgttactatg tcacattatg
    27121 aaccaccatt aaatatcgtt ttaaactatg atggatggta ttcacgacaa gttatcaata
    27181 tgtttgttag gtatgtagaa actatctgtg aaagatataa aaacaaagtg aaatattggc
    27241 ttacatttaa tgaagttgat tctatgattc gtcatccgta cactactggt ggtttagttc
    27301 gagaccgttt taaggataaa aactttgagg aagttatttt tcaagctatg catcatcaat
    27361 ttgtagctag tgccattgca acaaaaattt gccatgagat tattccaaat tcaaaagtag
    27421 ggtgtatgtt aacaaaatta acgtattatc catatacatg tagaccagaa gatgttttgg
    27481 caactcaaca aaaaatgaga agtatttatg catatagtga tacgcaggtt tttggtgagt
    27541 atccagtata tttactgtcg tattttaaaa ataataacat tcaaatagta aaagaagaac
    27601 atgatgatga aattatgaaa aagtatccag tagattttat ttcattctct tactatatgt
    27661 cttcttgtga ggcagctgat acaactggat tagatattac acctgggaat acgttattag
    27721 ctgtaaaaaa tccttattta gaaatgagtg aatggggttg gcaaattgat tcaataggtc
    27781 tacgtatatc tttgattgaa ttatatgata gatacagaaa gcctttattt atagttgaaa
    27841 atggattggg agctaaagat attttaactg aagataaaaa agttcatgac caatatagaa
    27901 ttaattattt aaaagaacac tttaaatgta tgttagatgc gattatcgaa gatggcgttg
    27961 aactttgggg atatacttca tggggttgta ttgatttggt cagtgaatca acgaaacaaa
    28021 tgtcaaaacg ttatggatat atttatgtgg atgctgatga ctatggaaag gggacatata
    28081 atcgatataa gaaagatagt ttttattggt ataaaaaggt aattgaaaat aatagtatag
    28141 attttaaatg accaatagaa ggtgagcaaa taaaatgaac tacataataa agaaagtttt
    28201 gaactcgagc gttgttttag ttcatgatgc aaagtataat gaatttattt tgctagggaa
    28261 aggaattgga tatggtaaaa aaacaggaga atatatttgt ggttccaatg ataatcaaat
    28321 gtttgtccct gttgaaaata caaaatcaaa acaattttta gaattaatgg atgatattcc
    28381 tgtcgacatt ttaaagataa cacaggaaat aatagtagag gcagaaaagt tattgaactc
    28441 atcttttaat aaaaacttat acttaatatt agcagaccat tttaactttg caattgaaag
    28501 gatgcgtaaa ggaataaaaa ttacaaatcg agtattttgg gaaataaaaa attattatcc
    28561 aaatgagttt aaggttggaa tggttgcaat ttatatggta gaggagagat tgggcattca
    28621 attaccagag gaagaagcag ctaacattgc tttccatttt gcaaatgcta tggcttcaga
    28681 tggaaattca tatgatacga ttaagtatgc caaagtaatt ggagaaataa ttaatatttt
    28741 tgttttttct ttgaatagaa ccttagataa aaagagtatg cattatatga gatttataac
    28801 acatataaaa ttttttgtgg aaagattttt ttcagatagt atgttaagca gtggtgatga
    28861 tttattattt aatcaaatga aaaggtcaaa tccaaaagaa atgttaattg cattaaaagt
    28921 tagagatttt ttagaaaaga aatatggaaa aaagttaaca aatgaggaaa tagctttctt
    28981 ggttgtacat atcgcacgag ttaagcaata gagtgaatta ctaatattat cttatacgtc
    29041 tacatggtag aaaattgaaa gaaggtaaag tatataaaga aactgagaca tattattttg
    29101 ttgttcatta tgtttatttt atgagtgtat cctgttaaaa atttaagtta atatttataa
    29161 ttaaaatata caaatagtta aaataagttt atgtggttat gaatttattt taactatttt
    29221 tttgtttaat ggaaaatatg ttttataact agaattttat tt
//
LOCUS       DAMJYX010000041        28736 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJYX010000041 DAMJYX010000000
VERSION     DAMJYX010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.041"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            97..663
                     /locus_tag="PC479_001917"
     CDS             97..663
                     /locus_tag="PC479_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2804464.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            968..1429
                     /locus_tag="PC479_001918"
     CDS             968..1429
                     /locus_tag="PC479_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804465.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1497..3743
                     /locus_tag="PC479_001919"
     CDS             1497..3743
                     /locus_tag="PC479_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804466.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3736..5568
                     /locus_tag="PC479_001920"
     CDS             3736..5568
                     /locus_tag="PC479_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804467.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6287..7564
                     /gene="hflX"
                     /locus_tag="PC479_001921"
     CDS             6287..7564
                     /gene="hflX"
                     /locus_tag="PC479_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2804468.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7797..8294
                     /locus_tag="PC479_001922"
     CDS             7797..8294
                     /locus_tag="PC479_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804469.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8344..8868
                     /locus_tag="PC479_001923"
     CDS             8344..8868
                     /locus_tag="PC479_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2804470.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            9180..10544
                     /locus_tag="PC479_001924"
     CDS             9180..10544
                     /locus_tag="PC479_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2804471.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            10683..11849
                     /locus_tag="PC479_001925"
     CDS             10683..11849
                     /locus_tag="PC479_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2804472.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            11966..12748
                     /locus_tag="PC479_001926"
     CDS             11966..12748
                     /locus_tag="PC479_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2804473.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(12867..13520)
                     /locus_tag="PC479_001927"
     CDS             complement(12867..13520)
                     /locus_tag="PC479_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2804474.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(13544..14908)
                     /locus_tag="PC479_001928"
     CDS             complement(13544..14908)
                     /locus_tag="PC479_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2804475.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            15566..16996
                     /locus_tag="PC479_001929"
     CDS             15566..16996
                     /locus_tag="PC479_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2804476.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            17099..18202
                     /locus_tag="PC479_001930"
     CDS             17099..18202
                     /locus_tag="PC479_001930"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2804477.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            18217..18453
                     /locus_tag="PC479_001931"
     CDS             18217..18453
                     /locus_tag="PC479_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2804478.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            18470..19519
                     /gene="vorB"
                     /locus_tag="PC479_001932"
     CDS             18470..19519
                     /gene="vorB"
                     /locus_tag="PC479_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2804479.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            19521..20261
                     /locus_tag="PC479_001933"
     CDS             19521..20261
                     /locus_tag="PC479_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2804480.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            20264..20803
                     /locus_tag="PC479_001934"
     CDS             20264..20803
                     /locus_tag="PC479_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2804481.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            20927..23026
                     /locus_tag="PC479_001935"
     CDS             20927..23026
                     /locus_tag="PC479_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2804482.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(23293..23808)
                     /locus_tag="PC479_001936"
     CDS             complement(23293..23808)
                     /locus_tag="PC479_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2804483.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(23900..25111)
                     /locus_tag="PC479_001937"
     CDS             complement(23900..25111)
                     /locus_tag="PC479_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2804484.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            25787..27229
                     /locus_tag="PC479_001938"
     CDS             25787..27229
                     /locus_tag="PC479_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2804485.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            27271..27624
                     /locus_tag="PC479_001939"
     CDS             27271..27624
                     /locus_tag="PC479_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804486.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            27815..28636
                     /locus_tag="PC479_001940"
     CDS             27815..28636
                     /locus_tag="PC479_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2804487.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT        11057 a         3256 c         5003 g         9420 t
ORIGIN      
        1 ataatttaat aaatataaat taaattatat gtgtatatat tgtactttaa tagtgatata
       61 ataataaaaa aatttttaaa gagggtgata ttacatatga gaaaacttga agagatatta
      121 gaatataata aatcatttgt agagaacaaa gaatatgaac aatatgtgac atcaaaacat
      181 ccaaataaaa aaatagtagt cttatcttgt atggatacaa gattaactga gcttttgcct
      241 aaggctatga atttgaaaaa tggagatgtg aaactaataa aaaatgcagg agctactata
      301 atgcatcctt ttggaagtat tatgagaagt gttttagttg caatttacga atttgatgta
      361 gatgaggtta tggttgtagg gcatcatgga tgtggtatgt gtaatgttga tacggataag
      421 ttacttggta aaattttaga tagaggaata tctaatgatg taatacttac acttcgtagt
      481 gctggaattg atacaaaaca atggttacat ggattcgatt ctgcagaaga atctgtaaaa
      541 gatagtattg atttaataaa agggcatcct ttaattccag atggtattat tgtacatggt
      601 ctaattatat cacctgaaac tgggaagtta gacgttgttg taaatggata tgaagataag
      661 tagagtatca taatgttata taatcaagat actgttgaat atgatgttgt ttcaaaattt
      721 attttgggac aactttttta tttgaaaata tatgaaaagg tttattttaa gctttacaat
      781 aaaaaatata agtagctgac taattaaaaa tcttactatt atttattaaa attataacta
      841 tatgatataa aaatatttaa taaatgaaat ttatatgaat tttttataaa tatattgaaa
      901 aaatgcatgc agtatagtaa aataatgctt agccgactaa gtaatataaa aaataaggag
      961 ggcaagtatg aatagcaatt ctgaagaaaa attaaatgat gttttcttgc aattaatgcg
     1021 aatatatttt ataagaactc atttttattt tgataaatta ggcttatatc atggtcaacc
     1081 ccaaatactt ttttctttaa agatgaaaga tgggcaaagt caaaaagaat tagcagataa
     1141 aaggaaagta aaagcatcta caactacagt tatgataaag aggatggaaa aagctggact
     1201 tgtggaaaga aaacaagatg aaaaggacca aagagtatct aggatttttt tgacagacaa
     1261 aggtttagaa atatgtaata aagttgaaga atttaacaag gaattggaaa gagagttttt
     1321 tttaggattt agtgaagaag aaaaaatagt acttagaaga cttctaatgc aagtacgaga
     1381 taatttaaaa gcaggatgtc ataagcatga agatgaaaaa tatttttaaa gtttaaaata
     1441 atgtttttca atttaatgtt attagactaa ttaatattac agaagggaag aaatgtatgt
     1501 ttaaattaat taaatattta aagaaatcgg cattatcaat agtaataata gtctgtctat
     1561 tggtaattca agcggtatgt gacctttctt taccagaata cacatccaat atagtaaatg
     1621 taggtataca acaaggtgga gtagaaaact ctgttccatc tgtgattaga gagagtgaat
     1681 taaataaaat tactttattt atggataaaa gtagtaagga taaagtacta gataattata
     1741 ctttgctgaa taaaaaggat tatgttaagt ataaagataa gtatccaggt ctaaaagatg
     1801 aatctcttta tgagttaaat actaaagaca aagacactat agatgattta aatgttattt
     1861 ttggtaaagc tattttaata gtatcagggc ttgagggcga tagtaaagag gtaaaagcta
     1921 tgaaagctca gttaatgagc aaacttccac cacaagctac tcaaagtgga gatgttgata
     1981 tatttaaatt gttatcagca atgccaaagg aacagttaga tacattgact aaagagatga
     2041 gcaaaggctt tgaaagtatg ccagagagta tgataacaca aagctctgtt tcatatgtaa
     2101 ggtcagaata tgagaaaata ggtattgata cagaaaaaac tcaaaataat tatatattat
     2161 ttactggtgc taaaatgctt ggtatagcaa tgattagtat ggtagctaca ataacagtag
     2221 gatttttagc agcaagagta gccgcaagtg taggtagaaa tttaagaagt ggagtattta
     2281 gaaaagttat gtcattttca aatactgaaa tgaatgaatt ttctacagct tcattaatta
     2341 ctcgtagtac aaatgatatt caacagatac aaatgttgat ggttatgttg cttagaatag
     2401 tattctatgc acctataatg gctataggtg gagtgataaa ggttttaaac acaaatactt
     2461 caatggcctg gattatagca gtagcagtag ttgctatctt atcattaatt gtagtgttat
     2521 tctcagtggt tatgcctaag tttaaactgg ttcaaaagct tgttgataag ttaaacttag
     2581 taagccgtga aataattact ggtattccag ttatcagagc ttttagtact gaaaaacatg
     2641 aagaagaaag atttgataaa gcaaataaaa atttaactaa aacaaatctt tttgtaaata
     2701 gagttatgac atgtatgatg cctgctatga tgcttataat gaaccttatc acagttctta
     2761 tagtttggag aggtgcatac agtgttgatt ctggagcaat gcaagttgga gatatgatgg
     2821 catttataca atatacaatg caaataataa tgtcattctt aatgatatca atggtatcta
     2881 ttatgcttcc aagagcatcc gtttcagcta cacgtataga tgaagtatta gtgtcagata
     2941 taagcataaa agacccagaa aaatcagagg cacaagagtt taaggaagat aaaaagggtc
     3001 ttgttgaatt taaaaatgtt tcgttcatgt atccagacgc agaagaacct atattaacaa
     3061 atatatcttt tacagcaaag ccaggagaaa caactgcctt tataggaagt actggtagtg
     3121 gtaagtcaac tcttataaat ttaattcctc gtttctttga tgttactgaa ggagaaatat
     3181 tacttggtgg agttaatata aaaaatgttt ctcaacatga tttaagggaa aaaattggat
     3241 atgtacctca aaagggagta ttgttttctg gtactataga atccaactta aaatatggta
     3301 gaaaagacgc aacagatgaa gaaatgagaa cagcagctga aatagctcaa gctacagaat
     3361 ttatagattc taaaccggaa gcttttaaaa ctgaaatttc tcaaggtgga acaaatgtat
     3421 ctggaggtca aaaacaaaga ctttctattg caagagcaat agcaaaaaat ccagaagttt
     3481 atatatttga tgatagtttt tcagcacttg acttaaaaac tgatgcagct cttcgtaagg
     3541 ctttaaaatc aaaaactgct gaaagtacag ttctaatagt tgcacaaaga attagtacta
     3601 tcttaaatgc agaacagata attgttttaa atgagggcga aatagttgga ataggaacac
     3661 ataaagagct tctaaaaaat tgcgaagttt atgagcaaat agctttatca caactttcaa
     3721 aggaggagct agctaatgag tagaatggga agaggtccta tgggaaaatc tatgggagca
     3781 ggacaaaaag caaatgactt taaagggact atgcgaaaac taattgcata tttatctaaa
     3841 tttaaaatat caataattct agtaattgta tttgctattg gtagtgcgtc gttttctata
     3901 gtaggtccaa aaattttagg aaaggcaact acaaagatat ttgaagggct agtgagtaaa
     3961 gtttctggag gaaacgtagg tatagatttt aatgcaattg gaaaaatctt aaccttttta
     4021 cttttccttt atttgataag tgcactgttc tcttttatac aaggatttat aatgagtgga
     4081 atttctcaaa aagtatccta taatttaaga aaagagattt cagcaaaatt ggatagattg
     4141 ccaatgaagt attttgatac taagacgcat ggtgaaatat tatcgagaat tacgaatgat
     4201 atagatactt taaatcaaag tttaaatcaa agtatgacac agcttataac atcagtaaca
     4261 actatgatag gtgttttgat aatgatgtta tctataagtg gaattatgac attagtggca
     4321 gttttaatat taccaatatc aatgtttgta atttcaagaa taataaagaa atcacaaaaa
     4381 tactttagat atcaacaaga atatttagga aatgtaaatg gtcaagttga ggaaacttac
     4441 agtggccaaa caatagtaaa agcatttaat cgtgaggatg aagtaattga agagtttgat
     4501 aaactaaatg atagcctgta taattcagcg tggaaatctc aatttttatc aggtataatg
     4561 caaccactta tgatgtttat tggaaacttg ggatatgtaa tggtatcaat cttaggaggt
     4621 tggcttgcaa ttaagaaaac aattgaggtt ggtgacatac agtcatttat acaatatgta
     4681 agaaacttta cacagccaat gactcagatt gcacaggttg ccaatttatt gcaatcaaca
     4741 gcagcagctt ctgaaagagt atttgagttt ttagaagagg aagaagaagt ccaaattgtt
     4801 gaaaatgcag tttcaataga tggtttagaa ggtaaaattg attttgaaaa tgtcaacttt
     4861 ggatataacc caaacaagac tataataaat gatttctcag taaatgttaa acctggtcaa
     4921 aaagttgcta ttgttggacc tacaggagca ggtaaaacta caatagttaa actgttaatg
     4981 agattttatg atgttaatag cggttcaata ctgattgatg gtcataatat taaagatttt
     5041 aatcgtagtg agcttagaga aatgtttggt atggttcttc aagatacatg gttatttagt
     5101 ggttcaatta tggaaaatat aagatatggt aagttgaatg ctacagatga agaagttata
     5161 gaggctgcta aatctgcaca tgttcataga tttattaaga cattaccaga tggatataag
     5221 atgaaattaa atgaagaagc aagtaatgtt tctcaaggtc aaaaacaatt attgactata
     5281 gctcgtgcta ttttagctga tcctaaaatt ttaatattag atgaagctac aagttctgtc
     5341 gatactagaa cagaagtatt gattcaaaaa gctatggata atcttatgga aggtagaaca
     5401 agttttgtta tagcccacag attatcaact atacgtgatg ctgatatgat tcttgtaatg
     5461 aatgaaggag atatagttga acaaggaaat cacgaggaac ttctaaagaa aggcggattc
     5521 tatgccaatc tttacaattc tcaatttgaa gaagatgaag caatgtaaca aaaaactatc
     5581 ttagcataga catgtaatta ataagataaa ataaatgagg gtgccttaaa atgaactttt
     5641 taattcataa ggcactcttt tgtgtatgaa ataaagtgta tttttatcat tttaacaatg
     5701 aaaaagagga ttctctatat tttgagatag ccacataatt ttatgtcttt acaaagtgtt
     5761 tattttatta taatatgtag taaggaaaat gttggaggtg attatcaaaa tgtatattta
     5821 attttattct ttaaatttta ttataaatag aatttaattt taatagttga tgattatatt
     5881 gaagtatatg tttttattac ttttattaat catgcaaata actacttaaa tttaagaata
     5941 gggttaaata taaaatgata attacatata agttaattaa actaatgtat ttttaatata
     6001 tatttagaat tttaatcctt cttatgatat aagtagcttg ttttgtatta cttagatttg
     6061 taaggagttt ttttattgaa aaaactgcat aatatatttg ggaggaggtg taagaaagtg
     6121 attttttata taagattgag aaaataatgc acgattatgg ttattataat agtaaataag
     6181 aagtgtcatt taaggataag ataaaaagca aaattcttga tgaatttaat tatagagcca
     6241 taatctcgtg ctatagatta aataaaaaat agcagataag gagaatatgg atatggcgaa
     6301 aaaaggaata acagtaggat taaacataaa taataaatct gaggacttta atgaactaat
     6361 gattgaactt gaaaacttat gttcagcatg tgatattgat gttgtaggga gtataactca
     6421 aaatgcaaag caggttaata gagcatttta cattgggaca ggtaaagttg aggaaatatt
     6481 aaacttaata aagaaagaaa atatagagat agttatattt tacaatgaac tttctacatc
     6541 acaactaaaa aatcttgaag aaaagcttaa ttgtgaaatt atagatagaa cggcattgat
     6601 acttgatata tttgcacaaa gggcaaaaac tagagaagct aaacttcaag tagaagtagc
     6661 aagtttaaaa tatatgttac caagacttat aggttcaaat gaaaatttag gacgtcaaag
     6721 tggtggagtt ggtactaaaa atagaggttc tggtgagaaa aaattagaac ttgaccgtag
     6781 aagaatagaa gaaaagataa catctttaaa tagggagttg gatgatttaa aattccaaag
     6841 agaaactcaa agaagtatga gaagaaaatc aaatcttcca aatgtagctc tagtcgggta
     6901 tacaaatgct ggtaaatctt caataatgaa taaactagta gatatattta aaaactctga
     6961 agaaaagaaa gtgtttgaga aaaacatgtt atttgctaca cttgaaactt ctgtcagaaa
     7021 tatagttctt gcaaataaca aagaattttt attatctgat actgttggtt ttgtaagtaa
     7081 tctaccacat gacttagtaa aagcatttag gtcaacatta gaagaagcat gtgaggcaga
     7141 cgtactgtta catgttattg atatttcaaa tccaagctat aaaagtcata ttaaagtgac
     7201 agaagatact ttaaaacaaa taggtgcaga tggtatacct atgatacatg tgtataataa
     7261 aattgattta atagatgtag aagtattgga tagaatttta gatagtatcg ataaagaagg
     7321 tatatttgtt tcagttaaaa aagatataaa tatagataaa atgataaaat gtatttgtga
     7381 tagcatattt aaagattatg taagatgtaa gttcttaatt ccatatgata aaggtcatgt
     7441 agtttcttat tttaacgaaa atacgagcat tataaataca gaatacagag aagatggagc
     7501 tattttagat gtagaatgta gcaatataga atataataag tataaaaagt atgctttaga
     7561 gtaaattttg aaaaacttat tatagcctaa aatatattta atttataatt tattataaat
     7621 tgattgtaaa tatgatttta tgaaacggta tagccataat gctatgccgt tttgccgttt
     7681 ataataaaaa taaatttatt gacataatag taactaatta ctataatata aagtaattag
     7741 ttactattta aaattatcaa aaaattaaaa tctaagataa agagaagtgt ataataatgg
     7801 atgcagaaag tttaatgaaa aaatatatgg tagatgatga tagtagaaaa caagctatat
     7861 ttgcaagtat atttgtaatg caaaataaac ttcaaactac atgtgataaa ttagacccag
     7921 aattaacaat gaaacaatgg ttacttttag ctattgcgga ctctgtagat gaaactctta
     7981 cattaaccaa acttggagaa ttaatggggt gctcaagaca aaatgtaaaa aaactagcta
     8041 ttgctttaga aaagaaaggt tttatacaaa ttgagcagaa tgaaaaagat tcaagagcta
     8101 tctgtcttgt gacaaaagat agagtgcaag agtattctaa aaaagtaaga ggtatgcagg
     8161 aaaaagtatt acagctttta tttgaagatt ttacagagaa agaagtggaa cagttatttt
     8221 ttggaattat gaaattaagt ataggaattg aaaatgtgaa aaattatgta gaaaataatg
     8281 acgttggaga ataagcaagt atcaaatcaa ctttgtatga catcagagag ggggataatt
     8341 tatatgaaaa atgtagtagt gatttataag agtaagtatg gttcaacaaa aaaatatgct
     8401 aaatggcttt cagaattatt gtcttgtgat atttttgaaa ctaagaatat taatatagaa
     8461 aaggttatga agtatgatac gattatttta ggaggaggtc tttatgcttg taatatagcg
     8521 ggaatttctt tcttaaaaaa aaattataaa gaattgcaga aaaaaaattt agtagtattt
     8581 ggcgtaggag ctgctccata tgatgatgaa gtaataaaga aattaagaga agtaaatttt
     8641 aaggatgaat taaacaaaat tccttgcttt tattgtcgtg gggcatggga tgagacaaaa
     8701 atgtctttta tagattatac tctttgtagt ttttttaaaa aatctatagc taaaaaagaa
     8761 cgtcaaggtc ttaaaccagc agaagcacct ttattgtctg gagaagcctt tgattggtgt
     8821 aataaagaaa atttaactcc aatacttgat tatataagag aactttaaga ggtatatatt
     8881 tgtaattatt tattatctaa agttagattt catagaaact tactagacat tatttataaa
     8941 ataatattat taaagatgga taatttaatt gtttcaataa ggttataatt ttaaaaaatg
     9001 tttattttaa aataaactat tttataagct tacttatact taataagggg ctttttttat
     9061 tggaagattt atgttatatt gagatgaatt tattatgata taaaaaataa tgttaggata
     9121 aatttaaatc tttttctaaa aatgtattag tataattctt aaagaaagga tttttaacaa
     9181 tggatataaa agagttagtt aaaatgcaaa gaaaatatta taatacaggt aaaacaagag
     9241 atatatcgtt tcgtatagaa cagttgaaga aacttaagtt agttgtatca caaaatgaag
     9301 aaaaaatact tttagcacta aagaaagatt taaataaatc ggattttgaa ggttttatga
     9361 cagaggtagg catgttttat agtgaattaa actttgctat aaagaatata agaaaatgga
     9421 gcaaaataaa aagagttaaa tcaagtatgg ttaattttcc atctatatcc aaagttgttc
     9481 cacaacctta tggtgttact ctcataatgt caccttggaa ttatccattt caattagcac
     9541 ttataccatt agtttggtca ttagcagcag ggaattgtgt tatattaaaa ccttctgaat
     9601 attctgtgtc aacatctagt gtagttaaag acatagttga agatactttt tcaaaagaat
     9661 atgttgcagt tgttcaagga agtcaagaag aaagtgaaaa gttactattg gaaagatttg
     9721 attatatatt ttttacagga agtactaatg ttggtaaaat aatcatgaaa tcagcatcag
     9781 agcatttaac tcctattact ttagaacttg gaggaaaaag tccttgtatc attttaaaag
     9841 atgctaatat agaccttact gccaaaagac ttacttgggg aaaactaata aatgcaggac
     9901 aaacctgcgt ggctccagat tatgttttag tgcatgaaga tagaaaaaat gagttaatag
     9961 agaaaataaa atattatact aataagtatt ttggggataa tgcatgtaat aatgagcaat
    10021 ttcctaaaat cataaatcag agacatttta atcggatatt atctcttatt gataaagata
    10081 agatagtcta tggtggaaat tataataagg agacattaaa gatagagcca actatagtag
    10141 acaatgttaa ttgggatgat aatataatga aggaagaaat atttgggcct atatttccca
    10201 ttttaacata taaagattta gatgaagtaa tacaaaaaat tatacaaatg ccaaatccat
    10261 tggcacttta tatttttaca aaaaataagt acctagaaaa taaactttta gaaatgattc
    10321 cagcaggcgg atgttgtatc aatgatacag ttactcatat agctacgaat tacttacctt
    10381 ttggtggtat tggtgaaagt ggtatgggct catatcatgg aaaagctgga ttcgatacat
    10441 ttactcacta taagagtgtg ttaaaaaaat taaatttgga tgtccctatt agatatgctc
    10501 catacgataa taaattaatt aaggttctta aaaagataat gtaggttata ttgtagaata
    10561 ataaatataa ataaaattga gtatttgtat tctgaatata ttttattttt aatttggtat
    10621 atttgttata aaagtggtga ttttgtgata aaatttaatt atatagaaat aaggaggaaa
    10681 aaatgaaaat taattggaat acaaaataca caacaatagc aatttataca tttataatag
    10741 ctgctagtag tattatattt tatctagtat catcacaaat tgatgtattt tctaataatt
    10801 tagatgctat atttacaaca ttacaacctt ttataatagg atttgctatt gcatatcttc
    10861 ttaattttat tttaaagttt tatgaagaca gaatttttat aaaaagtgag aaattaaaaa
    10921 aactaaaaca aagttcaaaa agaggcttgg gtcttttatt aacatatgca acagctacat
    10981 tgatacttta cttatttatg catttcgtat tgccacaagt aattgagagt attgttggac
    11041 tagcaaatga tatacctatg tatgtaaaca atgcaactaa gcttattgat aaattaatga
    11101 ctgatttaaa tcttgatgag caatacttta atttggcagt tgataagtgg aatgaatttg
    11161 ttacatatat tataaagttt gtaacagatt taatcccaat attaggaagt atgttaaaga
    11221 atgtagcctc tagtatatgg aatattgtac taggattaat tgtttcagtg tacttattaa
    11281 ttgataaaga aaaattctat ggattaagca aaaaaataac ttatgcagta tttacagaaa
    11341 aacaagctgc cagaatttta gaattaactc atagaagtaa ctatacattt ggaagatttt
    11401 taggtggcaa aatattagat tcattcatta taggtattct aacatttgta atacttacgt
    11461 tagttaaaat gccatataca ttattaatat cagttataat tggtattaca aatattatcc
    11521 ctttctttgg acctttattt ggagctatac catctacgct aatagtgatg tttgtatctc
    11581 ctataaaagc tttctggtta ttgttgatta tattaataat acaacagata gatggaaata
    11641 ttataggacc taaaatactt ggagattcaa ttggtatatc tgcattttgg attttgttct
    11701 ccttattggt tgcaggaaaa ttattaggtt ttattggaat ggttatagga gttccaatgt
    11761 ttgcagtaat ttattctata attaaggata ttgttgaaag taaattggac aaaaagggat
    11821 taccgacaga tacttccgat tatatgtagc taaaaaatat tatgtataat taaaaatata
    11881 taaattaagc aatatttaaa gcactgagaa agttaaactc ttggtgcttt ttagatataa
    11941 aattattata gaagaggtgt tttttgtggc atttatagaa tgtaaattta aatctaaata
    12001 tactggagga gaaactgata taattgtaat tttgcctgta gcaaatggaa aagaactttt
    12061 tagtggtaaa gagatatatg attttgaagg taaatttaaa acattatact tatttcatgg
    12121 tttagaagat gaccaaagct catggattag atatactaat gtagagcatt atgctaaaaa
    12181 taaaaatata gccctagtaa taccaagagt agaaactagt ttttatactg atatggcgta
    12241 tggacaccaa tattttactt ttgtaagcga ggaattacct aaatttgtta gagctatatt
    12301 tcctttatca gataaaaggg aagataactt tgtagctggt atgtctatgg ggggatatgg
    12361 agctttaaaa ttagcgcttt caaaaccaaa agagttttcg gcagttgcaa gtttttctgg
    12421 ttcgcctgat atgattcatg agttagaaaa tcaacaaata gaaaccaatg cagacattct
    12481 atttcacgat tttggtagac cagaagacgc tagaaatagt gagaatgatt tactttacat
    12541 acttaagaac cttaaagaaa gaaatgaaga tattcctaaa atttatcaat tttgtggtga
    12601 tagagatttt ttatataaaa ataatcagat gtttaaaaaa tatgcagatt ctctgggtgt
    12661 agatgttgaa tatgaagagt gtgtaggggg gcatgaatgg cgtctttgga atttatggat
    12721 aaagaaattt atagatataa ttggatagta cacttatggt attaacaggt atactataaa
    12781 atatctttaa atatgataaa gactgaaatc ctaaaatcaa attatgattt taaaatttca
    12841 gtctctaaga atttaataaa attttattaa gttcttacac gagttattat tggagtaaaa
    12901 ttgtcaacta tatctatata gtatttactc aattcctcta aaatctcttc gcataaatcc
    12961 gtactatgac caagattaat aagaaactgt tttgtaaaat gctttattac agcttgagaa
    13021 gatatatcgt acttgtgtga agtataaact ctaattattt caggtttcat agcaagtata
    13081 ccaatacaag aaagtattaa ttcatcatgt tctatagatt ctttatcatt aacatatctt
    13141 ttcatgacca tttcggttac acttctttga tagcttaaaa tagcttcttc ataagctata
    13201 tccaaataaa aatttctata tgcttcgttt tttagaagga aacaaaggtc agcattcata
    13261 gcaagaaaga acatcatcat agtaccttct tcatggtagt tatagattaa agactgcatt
    13321 tcatttctta tatcttcata tattatagct cctatttcgc ttttacttga gaagtaatag
    13381 cttattagac ctaaatttat attagctctt tctgcaattt gcctagttgt tgtcttatga
    13441 taaccttgct caaaaaataa ttctctagca gcacttataa tactagcttt tgtttctttg
    13501 cccttttctg attttgacat atttactgtg tatacctctc ttttcaatct ttttttgaaa
    13561 atttgctaac tataaatatt gtaatagaac cacctattat aggaaccaaa attctgtcaa
    13621 atacagtaag aataccaatc atatttaata atgccaatat aacacttact ataacagcat
    13681 agatagctcc tttttttgta gcacctttcc agcaaagacc aattacaaat ggtgcaaata
    13741 ttacacctgt acataatgga gctggccaaa acaataattc taataaacta tcaaatttaa
    13801 gtgctatgat tgcacctatt atagctgtca aagctgatgc aatttgagaa tactttagag
    13861 tattttttaa atcattaaat tctatattag gatgtaataa tttgttataa atatctcttg
    13921 atataatagc actcattgag gtcattaatg aatcggcagt agacattatg gcagccaata
    13981 tagcagctaa gaataataca gatattaaag gtggcaaagt agtcgatatc atttgaggta
    14041 aaacctgaac agatgatata tttggaaata aactaccacc aatagctcca attattccta
    14101 tagggaaagc aagaaataat agtataaatc ctgcaattac actactttta actgcatttt
    14161 tagcattatc agcacattga tatcttaaaa aactttcata agaaaatatt gagcctaaaa
    14221 ctaatggtaa aactgtatac ataaaatcac taatcgattt atttcctgga aagaatggtt
    14281 cttttaccat tgattgtaac ataagtaaag ggttgtcgcc aactaatttt aagcttatat
    14341 aagctactat tggcaaaaac actatacaaa tagcaagttg aataaatgct gatactgcaa
    14401 ctccccacat tcctgatgta taagtatata taatgataac tataactcct ataattacag
    14461 ctatatcata aggaatacca aatgcagtag atacagaacc aaaagaaatc aattgactac
    14521 ccataatagc tagtatagat attggtgtta caagactttg ataagctctt aaaaatacac
    14581 tatgaccata tctagtttca atataatcag caggtgttat atcacaagat aagcttctta
    14641 attttcctgc gaaaaatgca tttataaagc aagctaaagc tgcacctata gtagaataca
    14701 ttccaaccca aagtcctctt tcagctcccc attgaactcc accaactaag caccctcctc
    14761 caaaagatgt agcagcaatg gttgcagcta gtataggcaa aggcagattt ctacctgcga
    14821 ttaggaaatc ggatgctgtt ttaacttttt ttgaaaaata aaaaccaatc catatgtata
    14881 ttgcacacgt aacaattaaa agtaacatag tcttttctcc tttataaaat attttaataa
    14941 aatatacaaa atatatttaa aatatatttt atataaaagt agtacgatat ttgttttatt
    15001 ggttttagat taatatagct ataataattt atgatattag atggaaatta cgtaaaaata
    15061 gaaaattgaa aattctgaaa tttttgcata ttgatattta tctcatagat tcttttttgt
    15121 aattgtaaca cgcttttatt tttaaatcaa tagtacatgt ataaaaataa aaggaattta
    15181 aataagaaaa agatattttt aatatctgga tgtagtatag aaatatcttg aataaattat
    15241 tcttgaataa aattataaaa aattaaaatc actagaaata tgataaaata atttaaaata
    15301 cgcatttcaa tgacatttca aaacaagaaa ataataaaaa acaaacctaa attaagtcta
    15361 aatagctttt tagttaattt tagtagtaaa aaataaaact taaaaagtgt ttgacaatta
    15421 tgaattttga gaatataatt aaattaaaat tatacacata taaaaatata ctcctgaatc
    15481 aagaatgaga tatttaagat tcaaaatata tattattata cgtgaataat aaaaagtaag
    15541 taaattttat aagggagggg tagatgtgag tagtcaagaa agcaaaccaa aaggaaatct
    15601 tatatcaaat tttagagatg atatgagaga ccctaagaaa agagcaatat acacatattc
    15661 aagttctata aatttagcgt acaattttat aggttacttt gtatcaactg ttacaaatat
    15721 ttttttaact gatgtggcaa aacttggtgt aattatggca ggtggtatgc aaattataca
    15781 atctttcata aaaattgctg tagctcctat aacaggtaca gtatttgaca aacaaccatt
    15841 caaaaaagga aagtattatc catggcttag atatgcacca gcagcgttag cattaacata
    15901 tggaatattt ttcatagttc cattaatgaa aataagtcca gcattacttt tacctttgat
    15961 gacagcttta ttattaatag catcttgtgc tcagcaagta gttttcacta taatttatgc
    16021 tatttaccct gtagttgcta agacacctag agaccgagtt ataggctcaa ctactacgaa
    16081 tatatttaaa gaagttggta aattcttagt aggtttttca tatccgttat tattagtttt
    16141 atttgcagat gtattaggtg gagaaggaat gggttattta ggtacctact tattctttgc
    16201 agcaggatgc cttttagtat tttggttttc ttcaacagag ataataaaat ctggagcaga
    16261 agaagaagtt cttcaaaaag aagagaagcc aaagattaaa gcaagtgaaa tggcaaaagc
    16321 tttattcact aatgtaccac ttatgatggc attttgttta gaatttctta tatgtataag
    16381 atctatagga ttaggacctt tggctccata ttattttaaa tatgtaatag aagatgaacg
    16441 tggattagca gtatttttat caatcatgcc tttggtaagt gtagcattca tgtttttcgc
    16501 accagtgttt ataaaaatct gtagagagca aaaactagct tcaataattt ctttttctat
    16561 atgtgcacta tgccacttat tggttgcatg gactccttgg gggaaaacta ctttaggagt
    16621 aactatgttg ttggcaattg gtggaggttt ttcaaatgta gtatcaataa taaatcttaa
    16681 tttctttgcc ggttcttgtg attatgggca ttggaaatca ggaaaagact tacctggctt
    16741 aagtatgtca ctatatccag ttgcaataca agtaggagta ttacttgcaa ctactataag
    16801 aacagtttta atgaactcaa tgggatatca agctgatatg gtagtcacag aagcagttaa
    16861 atcaggtttt ataaatatga tttcttattc tatggcaata cctttaataa tagctgttgt
    16921 tatagcaata ctatatccag taagtgacaa gaaattaaac caaataagag aagagttaaa
    16981 tcaaagaaat gcttagttaa aagaaagttg agtagatggt taatttaagc tattaaagta
    17041 agtagacgca atagcgttta tataaatgaa aatacatata cagagggggc ttttaaaaat
    17101 gatagaaaaa ttattgagaa ataccgtaaa agaattacat caatatgttc caggtgaacc
    17161 aatagaaaaa gttaaggaaa aatatggtgt aaaagaaata ataaaattag cttcaaatga
    17221 aaatcctcta ggaccatcac caaaagcaat agaagctatg attgaaatgt taaaacaagg
    17281 tcaactttat ccagagccag aagctaacga acttagaaga aaattagctg aaaagctaga
    17341 tttaaaacca gaaaatttta ttgttgctaa tggtgcagat aacgttatca ctctaatagg
    17401 tgaggctttt ataaatagag gagatgaagt tatatattgt aacccaacat ttccatcata
    17461 tagaactgca actataaaaa atgaagggat accagtagaa gtacctttaa cagaagatta
    17521 taagtatgat ttacaaggta tattagataa gattacagat aaaacaaaat taatatgtgt
    17581 atgtaaccct aataatccaa caggaacaat tgtggatgat aaagagttag aagaattttt
    17641 aaagaaagtc cctgaaaata ttataactat attggatgaa gcatatatag aatttttaac
    17701 agttcctaat tatgtggatg gacttaagta tgtaagggaa aattataacg ttatagtaac
    17761 aagaactttc tctaaaatat atggtcttgc agggcttaga gtaggatatg gtattgctaa
    17821 agatgagata ataagaactt tatttacagt aaaagagcct ttctctgcaa atcgtgttgc
    17881 tataagtgga gcaacagcag cacttgatga tgaagagttt ataaaagaaa catatgaatt
    17941 aaatagagtt ggaatggctt actttaaaga agagtttaca aaaatgggat ttgatgttgt
    18001 agattcacaa tcaaatttcc tttatgtaga tatgaaaaca gatataccta agttatttga
    18061 agacttgaaa aaaagagggt ttgtaataag accaagtgct acacatgcac gtgtaagtat
    18121 tggaactatg gaagaaaata ggaaatttgt agcagtttta aaggaaatat taaatattaa
    18181 aggagagcca agtattggat aatagataga aatattatga aacggggtgt agatatgaac
    18241 ttaaaaataa acgcagagag atgtaaaagt tgtgaatact gcgttatatc ttgtaaaaaa
    18301 ggagcattaa aaatatcaag taagataaat aaagaaggtt atgctcatgt agaagttgat
    18361 gagagtaaat gcatactttg tggaatttgc tatcaggtgt gtccagataa tgtttttgaa
    18421 ataattaaag aagaagcatg tcaagaggct tagaaggagt tgggataata tgaaaaagat
    18481 gtggaagggg aaccacgcca ttgcagaagc agccctaaga ggaggttgtg aattttatgc
    18541 aggatatcca attacaccac aaacagaggt aatggaattt ttatcacata gaatgtctga
    18601 acttggaaga acttttatac agtccgaaaa tgagatggct gctatatata tggtatatgg
    18661 agcatatgca agtggaatga ggtctatgac gagttcttca ggaccaggaa tatctttaaa
    18721 acaagaaggg atttcttact tatgtgcaaa tcattaccct tgtgtaatcg taaatgtaca
    18781 gagatggggg cctggtcttg gctcattgga ctcagcacag acagactatc taagagatac
    18841 tagaggggga ggaaatggag attatcattt gatagtttat gcaccaaaca gtattcaaga
    18901 gactgtagat ttgatgtata actcatttga tgtagcggag aagtacagag tgcctgtcga
    18961 aattttgaca gaagcagctt taggtcaaat gatggaacca gtagaatttc cagagtttaa
    19021 gaagagagaa ggagatttag gttggactta tgatggaagt aatagagacc atgcaaaggt
    19081 tgcagataac caaaagccaa ctttttgtat ggagaaacgt atgagaataa ctgaaaatga
    19141 acaacaatgg gaagattatc aagtagaaga tgcagaatat gtgtttgtag cgtttggtat
    19201 accatcaaga acaactatga atgctgttag aagacttaga gaacaaggtg aaaaagttgg
    19261 aataataaga ccaataacag tatggccttt cccatataaa gcatttgaaa aggtaagtaa
    19321 agatgtaaaa ggatttatct cagtagaatc aacagacaca ggtcaattag tagaagatgt
    19381 tgctttagcc tcaaagaaag tatgtaaaga aaatgtacct gtgtatggtt tatttagtgg
    19441 aaatcatata ccaaaaacat tgcaagtaat ggacacttat agcaaaataa aatctggcga
    19501 aatcaaggag gtgttctaat atggctacag aaaaattaac acctagcgtt gtttcaatac
    19561 caaacaagtt ttgcccaggt tgtggtcatg gaatagtaaa tcgtataata gcagaggtaa
    19621 tagaagaaca tggatatgaa aaaaatcatg tattaacatt aggagttggt tgtgtctgca
    19681 atatgaattt tagctggaat ggtgataaga tgcaaactgc acatggtaga gcttcatcta
    19741 cagctatagg agttaaagta gcacttccag atacattagt aatgacttac caaggtgatg
    19801 gagatgctta tgttataggt ctttctgaaa ctttaaatac agcatataga aatcataatg
    19861 tgactgtatt tgtaataaac aataataatt ttgcaatgac tggtggacaa atgtcatgga
    19921 ccactatgcc gggtcaagtc acaacaacaa gtgtaaatgg aagagatata aagactactg
    19981 gtagtccact gaaagttcct gagatggtgg caaacttctt tgatgtagca tatgtagcaa
    20041 gaggttcagt tcatagccca aaggaaataa ttaatctaaa aaaatatata aaaaatgcaa
    20101 tagaagctca attaaatgga gagggatatt ccttagttga aatattagca ccttgcccaa
    20161 ctaactgggg tgtatcacta gagaaatcta tcaaatggat ggaagaagaa atagtgccat
    20221 attatgctct tggcgaattt aagcaaaggg atggtgaata gttatggaaa aagagctagt
    20281 atttgcaggg tttggtggtc aaggagtttt aactcttggt ctgattgtag ctgagtcagc
    20341 acttgaattg gggaaacaag taacttggat gcctgcatat ggtccaacta tgagaggtgg
    20401 aaaagcctat agtgtagtaa agttttctga tgagagtata ggtgggcctg atatggaaga
    20461 aatagatgtt ttagtagcaa tgaataaacc atctttagat tatataaatt tagttaaaga
    20521 aggtggaaca gtagttatta atacatctgc aatagatgag aatgtaacat taagagaaga
    20581 tataagtgta gttaaaataa attgtcagga attagctcaa aaagtaaaca atccaaaagc
    20641 agcaaatata gtagtacttg gagcattaat tgctaaaact gggttatttg aaaaagaatt
    20701 agctttaaaa actatgtgtg atttctttga ggaaaaagga aaaggaaagt ttaatgttca
    20761 aaatgaagca gcattcatgg aaggctataa taacgccatt tgataaatta cccttatctt
    20821 aagtgattta tctcaagatt taataatctt tagtgccaag agaaacttca tcttgagata
    20881 aaattcactt aaaatcacaa ctataaacat ataggagcgt gaatttatga gtttggaaaa
    20941 acttattaga ccaaaaagta ttgctatagt aggagtgact gataaacttg gttttggaag
    21001 aagtgcagca cttagtatag taaaaagtaa ggaaacagat agagtatatt atgttaatcc
    21061 caaaagagaa gaattgtttg ggagaaaatg ttataagacg atacaagaag ttccagaagt
    21121 agtagactgt gtagtagtat gtacgcctag aaatgtagtt ccatctgtat taaaagattc
    21181 tggagagtta ggaacaaagg cagcagtggt atatgctagt ggatttgcag aggaaggtac
    21241 agaggaaggt acagatttag aaaatcaact tatagaaata tcaaatacat acgatatgaa
    21301 aatattgggt ccaaactgca tggggttgct aaattgtata gataaggtta atctatgggc
    21361 aggaggttct aaatgggatt tagatactaa aaaacctggt ataggaattg taggtcaaag
    21421 tggatttata actgctgaaa ttgtttcaag tgattatttt aatatatctt atggtttttc
    21481 aacaggaaat ggaaatatag ttacactaga agatgcagta gatttccttg tagatgatga
    21541 ttatgcatca gtaatagcaa tttaccttga aggtttaaaa aatcctcaaa aatttataga
    21601 tgctttaaaa agggcagcac aaaaaagaaa gccagtaata atattgaaat caggaaggtc
    21661 agaaaaaggt gctatttctg ctgcttctca tacaggaaat ttagctggtt cgagtaaagc
    21721 ttttgaaagt atttttgaaa aatatggagt aatttctgtg gaaaatttag aacagtttat
    21781 gtgtttagca caagctttta gtgttttaga tggtaattta cctgcaaatt ctaattttgc
    21841 agcaataaac ttctcaggag gagaaaatac tatttgtgca gatttagcag aagaaaatgg
    21901 tgtagaactt gcagaaatat cttctgaaac taaagaagaa atggaaaaat atcttccagg
    21961 atttgcaaca cctaaaaatc cactagatgc tacaacagca ttattccatg aaaaggatat
    22021 gatagtaggt ttattacata cctttgaaaa ggatgatagt gttggattta ctatggtagg
    22081 tgcaaatata agagatgaag aaaatgaaat gcatgaaacc ctttgtaaag ctgtaagtga
    22141 agctagagaa caaggattga aaaaacctgt gtttgcagtt cctacacttg aaggaaatag
    22201 atatttagat tataggacta gattagaaga taatcaagtg cctataatgt catcagttgg
    22261 aactacattt acttgtttta ataaaatggc taagtttata gattatgatt attcaaaaag
    22321 aactttagaa tttaaagctg ttaagaaaag agagtcaaat aatgtagtag cattatcaga
    22381 attagatagt aagattgaga tgaaaaaata tggagtacca gttccaggac aaggtaatgc
    22441 aaaaagtatt gatgaattag atgaaatctt aaaatatatt aagtatcctg ttgttttaaa
    22501 aataaattct tctgaaatat tgcacaaatc tgatgttgga ggagttaaaa taggaattaa
    22561 gaatagagat gaggcagtag atgcatataa tgagatttta actaatgtaa aaaaagcaaa
    22621 accagatgct aatattgatg gtatattagt tcaagaaatg gtagaaagtg gtatagaaat
    22681 tattattggt ataactaatg atgaccaatt tggaccaatg ttattagtcg gtttaggagg
    22741 agtatttgta gaggtattta aagatactac attatatcca ttacctataa atcatgatga
    22801 agctattatg atgcttaaaa aactaaaatc atttaagctt ttaaatggct atagaggaag
    22861 tgagccatgt gatattgatg ctcttgcaga tatgatggtc aagttaggaa aatatgctta
    22921 tgaaaataag gatgaagtca aggaaataga cttaaatcca gtgtttgtat atcctaaagg
    22981 aaaaggcgta tgtgcagtag atgccttaat agttaaatat aaatagttta taagttttgg
    23041 tattcaacca taattaaagc accaactgaa aatcacattt tgtaagttag atagtgtgat
    23101 tttcagtcac cccaaattta atttaaaaat attttaaaat gaaagtatag ttttaagttt
    23161 cattgtgaaa tagcactgta taaacaataa aatactacgt ataaaacgaa taataaaagg
    23221 tgttttaatg ataagaatca atcttgattt tcattaaaac accttcttta ttatattaaa
    23281 aatttttaaa cgttatattg tcacaggtgg gtttgttgga gctgcatatg tacgattagc
    23341 aaattcttgg tcaacaaaga atagcccacc ctctccagca agtttaacac gttttatatt
    23401 gtcttcacaa tttgtttctt cttctgcttg ttcagatata aaccaaagta agaattgaga
    23461 agttctaaag tctgattcag agtttgctac agctgctaag ttgtttatta aagctgtaac
    23521 ctttttttca tgagctaacg tacgctcaag aatatccatt gcagaagtaa agtctgaatc
    23581 tggcattggg atagcatcaa gctgaacctt tcctcctaca ttatgtaagt atttgtaaaa
    23641 aaacatagcg tggtctgtct cttctttata ttgaacataa aaccaattag aaaatccttt
    23701 aagtccttct gcttcaaggt aagaagacat agaaagataa agatacgcag aatataattc
    23761 atggttaatc tgctcattaa gtaatttttc cattttagct gaaatcattt ttatacctcc
    23821 aaattttaaa tgctcttata aaatataata atattaaatg gaaaaatatt caataaaagt
    23881 gtttaaatat tttacatatt taataaaaat ttatactacc attaaaaaaa ttataactta
    23941 caatggctat agagccaatt aatggtgcat ttgctccaaa atttgaatgc cttacactta
    24001 tttttttata tttagaaaaa cttgctgaag cagtcaattt ttttaataat gtattttcta
    24061 taaatgtacc aggtatatta aagtcatagc ctattattat agaactacaa tcaatcaatg
    24121 taagtgtatt aactaatgca tatgatacgt atgtacaaaa ttcatcaagc aaaaacatgg
    24181 caagactatc tttattattt gcctcatcta ttaacttgac taaagaaaaa tctttacagt
    24241 taattaatgg ggagttagga taaatatggc tcaatgattc aattctttta attagattac
    24301 tgacacttgt ataataatct aaacatccat tgttaccaca actgcacaat ggtccactaa
    24361 aattaattga tgtatgtcct atttctccac tttgtcctag gttgccagta tgaagtttat
    24421 tttctagaac tagaccagct ccaataccat tcattatgtg taggtatata taatttgata
    24481 tatttttacc taaaccatac attttttctg ctaatgcacc cgaattagca tcatttatca
    24541 aaaatattgg caaatcagac atttctttta taaaatctac tatatttagg tttgatacat
    24601 ttccaaaatc tgggggattt agtatggtac catttatatc atctactgga cctattgaag
    24661 aaataccaat tccaattata tctctcttgt tgattttcat tattttagta aacattcttt
    24721 ttattatatc ttttaaaaca tcatttgata gaaatttagg gtaagtttca gaaagttgtt
    24781 ttacaatatt accttttaaa tctgaaataa ttaccttaca tatatttctt cttattagga
    24841 ttccacaaat acaaggcgat atattagaaa tatctaataa aataggttta cgacctgatg
    24901 aatgtgcatt taaagttgat gactctattt ccctaatcat attttcatta ataagttcag
    24961 taactagatt tgataaagtc attttagtaa gtcctgtaga cttagctaaa tcaactctgg
    25021 acataggtgt atttgtagaa agtaatttca atattattaa cttattttta atttttacat
    25081 ctttgatgtt agaaccttgt ttgttactca tcataaatct ccttaaataa agttaagtgt
    25141 tttatttatt ttcaagtata ctatcacctt ataatagagt aacatttttt tacttgaaaa
    25201 tatcattaat tagaatatat cacattatat atcataaaac atataaaaaa atattagaat
    25261 taaattaaaa gtgaaaagta gattgatata taaatagtaa atacttaatt aaaaaaagat
    25321 aatgatagca ataattattg ttaccaagaa tctactactt caagtgttac cttaggtatt
    25381 taagttgata gtggtttgca cgtaataaac ttgatgtaat atatattctt aataatatcg
    25441 ataaaagatt tacttaaaaa taatatagat tatgtaaaca tataatctat ttattgtata
    25501 caataaaatt tcattttgta aaacagtgaa ttaaatatgt aatatagata tcgattttta
    25561 ttacaaattg ttactaaata taggtacatt agaacaaaaa tctagtaaat tgtaactaaa
    25621 atgtaaattt ttcctttaat ctatacaata ttatggaaac tttttgtaat ttatgatata
    25681 ataacaaata tataattatt aaattataat aaatgactat agcgtagatg taatactatg
    25741 ttatagtcat ttttgaatta aacttgatat atcaaggggg aagaatatga gaacaattag
    25801 aagagtttta gcattaggtt taacattagc tatatttcta ataaatgtac ctaatgtaga
    25861 tgcattaaca tcagatacaa ttaaaggtaa caatatatat gagacggctg gattaatagc
    25921 agataaaaag agttatgata cagcaattat ggtaaatatg gataattcta tagcagatgg
    25981 tctttcagca agtggtcttg caggtgctgt tgatgctcca attttgcttg cacaaaaaaa
    26041 taagatacca aatgaaacaa aacaaagatt aaaaaatgtt aaaaagatat acataatagg
    26101 taaagaatta tcaataagta agtcagtaga aactgaacta aaaaacacag gggcacaggt
    26161 aactagattg ggtggagatg acagaataaa gactagttat agtgttgcta aagaggtaaa
    26221 tggtatcaaa aaagttgatg aagtgatatt aactaatgca tataaaggag aagcagatac
    26281 aataagtgct gcacccgtat cagtaagaga tatagctcct atagtactta cagatggaaa
    26341 gagtgtgcct ttttcaacaa gcaatgtaaa aacatatgca gtaggtggga gtatatcaat
    26401 gagcactagt ttagttaata aaactaatgc taaaagactt ggtggttcag atagatatga
    26461 tacaaataag aaagttataa aagagtttta tccagatgca tcagaatttt atttgagtga
    26521 tggatatgat ttagtaaatg cacttacagg ttctacaatt gctaaggaaa atccaattgt
    26581 attggtgtca gaaagtagtg ataagtctat attagcagga gcagataaga ttactagatt
    26641 aggttcaata agtgacagtg tgtataataa gtgtgtttct gctgcacaaa ataatggcga
    26701 ttcgtctaca aaaggtgagt cacctatgaa aaatgagaca agcatattag gtcagccaac
    26761 agctagttta gaagcgtgct taaaatgggc aaaatctaaa aaagctaatg atttatttat
    26821 agagttaata ccaatattat atgatactgc tgttcaggaa ggtgttaatc ctgttttggc
    26881 agtagctcaa tctgcaaaag aaactggttt ttgtaatttt ggtggagtat tagatgcatc
    26941 atttaagaat ccttgtggac ttaaaacttc tgtaggtggt tctgatactg ataagaatgc
    27001 ccattcaaga tttgatactt gggaagaagg aatattagct caaattcaac atttatgctt
    27061 atatgcagga caagatggtt acccactttc aaatccagta gaccctagac atgaaaaatc
    27121 tttatttggt aaggctaaaa cagttgaaag tttatctaat aattgggctg gaggtcaata
    27181 tggacaagat ttagtaagaa tgatgggaga aattgaggca acaaaataat tgaagattat
    27241 caaatatcca ataaataagg attgattatt atgaaaaaga gtaatatttt tagtatcata
    27301 tttcctacag tcataatggt gctaatgttt ttaacttttg ctacagatat atttaatatt
    27361 ccagatattc aatctaaaag tatttttgtt gtgggaatgg ttttgatttt tccaatttct
    27421 tttttgatac aagggataat ttgtgtatta aataagacta attggatttt atctcttatt
    27481 gtttctttag taacttatat agtattaatg tatatatttt taaatgattc tgcatatata
    27541 tatgttttac tttatacagc tttttatatg attggacata taacagctaa actctattac
    27601 aaaattaaga cttttaaaaa ttaaattgaa gtatataaaa gtaacattag gagaagtatc
    27661 cttaatgatt ttctaaaatc agaaataggt gcttctcttt tttgtaaaaa taaaagtatg
    27721 taaataaaat ttaaccatta taattttctt ttaacaaatg tatacaataa acttgttgta
    27781 taattaagat agataaaata aagggaggga taatatgaat ttaatagata atcatattca
    27841 tactaatttt tcaagtgatg gaaaggattc catggaagat acaataaaaa aagctataag
    27901 tattggtgtt agatatttga cttttacaga ccatttagaa catgatgaag atagaggatt
    27961 ttgcataaat tataatgact atgttccagt atttaataag tttaaagaaa aatataaaaa
    28021 ggacatagag ttattactag gtgttgaagt gggttacaga aaacatctaa aaaatgaaat
    28081 agaagaaata ataaattcta atccttttga ttttgtattg tgctcaactc atacaattga
    28141 caatgtacct gttccatcaa aggcgtattt taaaggtctt agcaaagaag atgcgtatta
    28201 taaatatttt aatagcatac ttgaaactaa tcgtgaattt ggagattaca atatatatgg
    28261 tcatttggat tatatatcta ggtatggaat atattcagat aatagagtta tatacaatga
    28321 ctttaaagat gttattgatg aggtcttaaa gtcaataatc aataatggga gtggtataga
    28381 gctaaatact tctggttata gatatggact aaatgctata catccaaatg aagatattct
    28441 aaaaagatat aaagaacttg gaggatttat agttactgta ggttctgatt ctcatagggt
    28501 agaagatata tgcaaagatt ttgatgtggc ctatgatatg cttaagtatc ttgactttaa
    28561 atatgtatct ttatttaaag aaagagaaac ctattttgta aatatagaaa aagttaaatc
    28621 taacaatata gcttagagtg tgttttgaag taaattaata gatagttaaa aagttaatag
    28681 ttatagtatt gtatcctcta gaaaaatact agaggttttt attttgctaa aataac
//
LOCUS       DAMJYX010000042        28180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJYX010000042 DAMJYX010000000
VERSION     DAMJYX010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.042"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            681..1643
                     /locus_tag="PC479_001941"
     CDS             681..1643
                     /locus_tag="PC479_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2804488.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            1722..2351
                     /locus_tag="PC479_001942"
     CDS             1722..2351
                     /locus_tag="PC479_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2804489.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            3184..3597
                     /locus_tag="PC479_001943"
     CDS             3184..3597
                     /locus_tag="PC479_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804490.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            3665..4081
                     /locus_tag="PC479_001944"
     CDS             3665..4081
                     /locus_tag="PC479_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804491.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            4082..4294
                     /locus_tag="PC479_001945"
     CDS             4082..4294
                     /locus_tag="PC479_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804492.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            4691..4861
                     /locus_tag="PC479_001946"
     CDS             4691..4861
                     /locus_tag="PC479_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804493.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            5404..6282
                     /locus_tag="PC479_001947"
     CDS             5404..6282
                     /locus_tag="PC479_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2804494.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            6318..8213
                     /locus_tag="PC479_001948"
     CDS             6318..8213
                     /locus_tag="PC479_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2804495.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            8481..10388
                     /locus_tag="PC479_001949"
     CDS             8481..10388
                     /locus_tag="PC479_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2804496.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            10378..10827
                     /locus_tag="PC479_001950"
     CDS             10378..10827
                     /locus_tag="PC479_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2804497.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            10927..11814
                     /locus_tag="PC479_001951"
     CDS             10927..11814
                     /locus_tag="PC479_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2804498.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            12219..13667
                     /locus_tag="PC479_001952"
     CDS             12219..13667
                     /locus_tag="PC479_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2804499.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            13693..16083
                     /locus_tag="PC479_001953"
     CDS             13693..16083
                     /locus_tag="PC479_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2804500.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            16085..16756
                     /gene="pgmB"
                     /locus_tag="PC479_001954"
     CDS             16085..16756
                     /gene="pgmB"
                     /locus_tag="PC479_001954"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2804501.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            16795..18165
                     /locus_tag="PC479_001955"
     CDS             16795..18165
                     /locus_tag="PC479_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2804502.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            18512..19354
                     /locus_tag="PC479_001956"
     CDS             18512..19354
                     /locus_tag="PC479_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2804503.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            19499..21427
                     /locus_tag="PC479_001957"
     CDS             19499..21427
                     /locus_tag="PC479_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2804504.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            21488..22915
                     /gene="ascB"
                     /locus_tag="PC479_001958"
     CDS             21488..22915
                     /gene="ascB"
                     /locus_tag="PC479_001958"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2804505.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            23326..24102
                     /locus_tag="PC479_001959"
     CDS             23326..24102
                     /locus_tag="PC479_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2804506.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            24118..24483
                     /locus_tag="PC479_001960"
     CDS             24118..24483
                     /locus_tag="PC479_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2804507.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(24659..25408)
                     /locus_tag="PC479_001961"
     CDS             complement(24659..25408)
                     /locus_tag="PC479_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2804508.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            25606..25758
                     /locus_tag="PC479_001962"
     CDS             25606..25758
                     /locus_tag="PC479_001962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804509.1"
                     /translation="MRRDNNLLMKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFV
                     FLFTIT"
     gene            25886..26311
                     /locus_tag="PC479_001963"
     CDS             25886..26311
                     /locus_tag="PC479_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804510.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIG
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            26343..27530
                     /locus_tag="PC479_001964"
     CDS             26343..27530
                     /locus_tag="PC479_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2804511.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            27722..28126
                     /locus_tag="PC479_001965"
     CDS             27722..28126
                     /locus_tag="PC479_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2804512.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT        11221 a         2888 c         4839 g         9232 t
ORIGIN      
        1 tatttatagt catttttgta tttaacttaa aagttttgaa ataattttca tatagaaaag
       61 agaatttttt attaaaagag attaatggaa ttgatatcat taattcaatt ttagtagtta
      121 tgtttagaag atgattttaa tctatgtata gttgtttttg ttagaataat aatttgtaaa
      181 tacaagccaa acactcaata ttatgttgag tatggaaata tgattgtata ttcttcagat
      241 aaaaatttga atttgaagtg ataattggaa ctaatttatg gaggttatgt taaaaaatca
      301 tagattctat taggtgattt tttcagtttt ataaaaaaca atacccatct gatataaaaa
      361 tagatttttt taaattaata tcttttatac tattaaacca ttaaagatga tagtctttcc
      421 tcataatata tttatggaag aaagaagatt tagacttatt gtaataatat tcaagggagg
      481 tagctgaaaa tatatcaact aaaatagata tactactatg taatttaatg gataacttaa
      541 ctatagaaaa agactatcta taaatatttg aaataaatct tagtataaat tattagcagt
      601 ttactaatgt ttttttgtaa aaaatcaatc aaaaagttat ataataactt tatataacaa
      661 atagaaagag gtgaaactac atggctaaaa ttgacaatat gatgtctata ctgtggatgt
      721 taaactctga caaaaaaatt acagcaaaac agatagctga aaaattagaa ataaatataa
      781 gaacagtgta ccgatatatt gatgtacttt ctgcgagtgg agtacctata atttcagata
      841 caggtcataa tggtgggtat agccttttaa ataattttac taaagcacct cttttttttg
      901 atatagaaga gcaaaaatct ctacttcatg cagcactatt tgctaaaaaa gaaggatact
      961 tcttagacga agcattagat aaatcgatat taaagttaaa aatgtattca aatcaaaaac
     1021 aagaagatat tttaacaaac cacttaagag cccttgaagt aataaaacca attgggaaag
     1081 tgtctataga atcagtactt aaaaaattgg agaatagtat attaaaggag ctatctgtag
     1141 aaatcaacta tcatgcaggt cgtaaagtaa aatcaaaata tagaattata aatccatatg
     1201 gaattgttca ctggaataat aattggtacg tagttgcatt ttgtaatatg agaaatgagg
     1261 ttcgtagttt cagagtggat aggattagtg aaatcataga aacatcaaat acatttaaca
     1321 gaccgacaga attttcagca agtgaatttt ttataaaagg aattatacct gaaataaaag
     1381 atgaacaaaa tataattcaa ttagttatag aagggagtat agattcttta gatagtttaa
     1441 gccaacattg gtttttaggg cactacttga aagaaagaac acctaataaa ataatattta
     1501 tgcttgaaga ggaagtaata tgcaagtaca taccaaatat acttttacct tatagaaaga
     1561 caatacaagt aattgaacca ctaagcctta ggaagaaaat aattgaagat ttactagaat
     1621 taattgatta ttatcaaatt taatagctta actgactgaa tatgtcagtt aagttatttt
     1681 ataattagat atataaatta attacataag gagtaaaaaa gatgaaaaat agaaaagtat
     1741 atatgtatgt atttgatact atgtcagatt gggaagtggc atatttaact gctgaattaa
     1801 atacaggaag atattttaaa aaaggattaa agccactaaa agtaattact gtaggaattg
     1861 ataaaaatcc tattataaca atgggtgggt taaaagtttt acctgaaatt acagttgatg
     1921 agtttaatat ggaaagtaag gatttattaa ttttgccagg gggaaatact tggttatcta
     1981 tgaatcatga gataatatta gaaaaagtta aagaagcttt aagacaagat tctatagttg
     2041 cagccatatg tggagctaca ttaggcctag ctaaaaaagg gctattagat tttagaaatc
     2101 atacaagtaa tgacttagag tttcttaaaa tggtaatacc aaattactct ggagaaaaat
     2161 attataaaac ggaatctgta gttagtgatg aaaatttagt tacggcttca ggaattgctc
     2221 cgttggaatt tacgtttcaa gtattgaaac ttttagatgt ttttgaatca gaagcattaa
     2281 attcatggct aaatctttac aaaactcatg actcaaaata tttttttgaa ttaatgaatt
     2341 gtaacaaata aatttaaaac ttatttttca atttaatatg gtatagcatt gtgatataaa
     2401 gaacaaaaaa ttacaatctg gtggaataga gtagtggttt ctaatataga tttctttttg
     2461 gtgaatttaa attttattta aatttaaatt ctaaaccact ttctatatag ccaacagaaa
     2521 agtgttaaaa aattatcata tatataatta ctacttatag taacacaaat agatctaata
     2581 actcctataa taaaatttat cattagttac atatagaata aacctcagtt ttgattactg
     2641 taggaactag gattatatga agctaccact catagatttc agttttttct gttgtatcat
     2701 agcatagaat agtaaattta tctgaaactt tattttaaat ttagaatttg catatagata
     2761 aattatgggc attattctga tgattcgtct ccaataatta aaaaaagaat gataaaatac
     2821 ttgaaaatac tagtctttat tgactagtgt ttttttattt cagtgataaa atataccaat
     2881 tatacttcag ttacaggaaa tgatgttttg agggtacatt gggaggcatt taggttgtat
     2941 aattataagt ttcaatgtta tatgctcatt agaataatag ttagtaaatg atatgattga
     3001 gatgatagat gaaataaact aatttagtac tgtattgtaa tgtttattat aaaatctttt
     3061 ataaaatata tttatgaaga tattattgaa tattttcaat attctattgc aatttatttg
     3121 gaaaagttat atatttataa cataaggtgt tataaatata taacttttaa ggggtgattt
     3181 tatatgtcta aatcagagct tggtgttaaa acaaatcttg tttcagagtt acttatgata
     3241 tcctattttt ttatagactc attattaaaa aagtttattt ttaaagaggg aaatctttgt
     3301 attactttta taattttatt cgctttttgg gcatgtgact tcatgtataa taaaaaatat
     3361 aatgatttag ttgacgaggc gtcaaaactt atgttattaa aggttgatca tattgcatca
     3421 aaaataatgt tatattctat tttgctaata gctgttttct tttcagcaaa ttatacaaga
     3481 ggtttggata tatcaaattt ggatataggt gtaattctct tagctatttt atttatacaa
     3541 tctttatcta aattaatttt gtatatacat tttgatagaa agggaatatg caactaagtg
     3601 ataatattga taaaaacgaa aattaaagag tatagagaat ataattttta aggagtgatt
     3661 ttatatgtct aaatcagaaa ctggtgtaag atcaaagctt atcctggagt tgcttgtaat
     3721 aacttatttt tttatagctg tattagccac taagtatatt tttaaaggtc caagcactgc
     3781 ttctcttcca gggttgagtt ggttaatagt ttttggatgt tattcaattt ataataaaaa
     3841 atataatgat ttagttgatg aatcatctga atttatacta tcaaaggtta ataagattgc
     3901 aataaaaata atgatatatt ctatagtaat aatatctatt ttctttgcaa caccttatac
     3961 aagtaatata aatatatcaa acctagatac aggtataatc ctcattgcag ttttatttat
     4021 acagtcttta tctaaattag tgttgtatat atactttgat aaaaggggga tatgtaacta
     4081 agtggcaata ttgatgaaaa caaaaattaa agaatacaga gaaaaactcc ttatgacgca
     4141 aaatgaactt gctaaattgg ttggggtaag acgtgagact attgtacact tagaaaatgg
     4201 gaaatataat ccttctttga agttagctat ggatatcgct aaagtattcg atactacagt
     4261 agaaaatttg tttgaattta ttgaagaaga atagtggaaa gtttataaac atttataaga
     4321 tggataataa aatatagtta tttaattatt aagtttgtta aaaatattta tataatataa
     4381 tatatgatta aggtcagatt ataattgttt taagataaaa tgtaataaaa tttaatggag
     4441 aaattttata ggtagataaa ttttaatatt tataagatta tgttaaggaa acacttaaaa
     4501 attagattaa aattgtgggt atttaaatca actaaaagat attgactatt tagtcaatat
     4561 ctttttttat taaaaaggaa tatacatata aataggttag attaaaaatt gggataaata
     4621 tgatttatat agcatcattt aattaaaagg agctatatta gtattcattc atacaattgg
     4681 ataatataca atgtcagcct tgctttatgg tgtttgtaat aaatctattt cagtttctat
     4741 tgtaattaat atgcaagaat ttaagatagg agtatctatg gcttatggtg tattagtaat
     4801 tggtatatgc tatatagcat taggaataat ttttaaatta gatagaaaaa gatttacata
     4861 agataacaaa ggctaattta taaattggtg cttagaaaac caaatcactt tattagctag
     4921 agatgaattt ggagggtgtc actttggtgt tttatgtaaa gtttatggaa gtaaaacatt
     4981 agataatcgt gagtaagatt tctataaatc gtatatacta ttggaatcca agaaaaggta
     5041 ttggccaaat agctaatatc tttttaattt ttttgactct aatatagcat taactattgc
     5101 atgagtatct ttaaatttat agttaatgta tcttaattgt ttattataaa atctaggaag
     5161 gctaaagcat aaaacactta aaaagagaag atgtacatga gaaatataat taactatgat
     5221 gcagacttct aaaaattatt tagtgaaaag gaacttagag tgtataataa aagttccttt
     5281 tcactaagga aaacgattgt tattttctga cattcattac cctgatatta tttaaatata
     5341 aacaacaaca aaattaacaa gaagcaaatt caacctaaaa tttaaaatat cgggaggtac
     5401 aatatgttaa taaacatgaa ggaaatgcta aaagtggcac aagaaaatca atttgctgta
     5461 ccagcattta atataggaag tgggcaaata cttaaagcag tagtacagag tgctaacgaa
     5521 aaaaatgcac cagtaatact tgcaatacac ccaaatgagc taagttttct aggagatagc
     5581 tttgtagcta gttgcataga agaagcaaat aagagtaaag tacctatggt aatacactta
     5641 gatcatgggg aaaataaaga acaaatatta agggcaataa gatgtggatt tacatcagta
     5701 atgattgatg gctcacattt accatatgaa gagaatgtag ctatatctag ggaaatagta
     5761 gaaatagcaa aaggattaaa tgtgtccgta gaaggagaac ttggaacaat aggaacaaca
     5821 ggaacatcaa gtgaaggtgg tactgatgaa ataatatata cagattcaaa attagctaag
     5881 gattttgttg aaaaaacagg agtagacaca ctagctattg caattggtac agcacatggt
     5941 atatatccaa aaggttttaa accagagctt aaactagatt tattaaaaga aataagagaa
     6001 gtagtagata tacctttagt tcttcatggt ggttcatcaa atccagatga agaaatagca
     6061 caagcagtta aactaggagt ttgtaaaata aatatttcga gtgatgttaa gtcggcatat
     6121 tataagaagt gcagagaact attagaacaa aacccaagct tatatgagcc agatactata
     6181 tatccaccat gtataaaagc tgcaagagaa gttatagagt ttaaaatgaa tctattcaat
     6241 gctatagata agttaaagtg cttttataaa aataaaaatt aattatgagg taacaaatta
     6301 aaaggagggg agaatttatg ataggtaaaa tactacgaag cgattgtatt gtactagata
     6361 aacaattaca aacaaaaaaa gatgtgatta attatatggc aaatcatttg taccatggaa
     6421 attatataaa taataaagaa aagtttatag ctgatattaa taaacgtgaa aaagaatcat
     6481 caactaatat gggtaattta atagctatac ctcatgctaa gtcaaatgta gtaaatcaac
     6541 ctgtaatgat gtatttaaga acagatagag agattacaga taatgatggt caaaaactta
     6601 ggtcattttt tatgatagca gttccagaat caggttcaga tgaacatctt aagattatat
     6661 ctaaactagc tacatatcta atggaagaaa attttcttaa gcatctacta agtgcagaaa
     6721 ctccatacga aatattaagt atatttaata ataaagctgc agaatcagga gattttagta
     6781 ataatcaaaa taaaggtctt atagttggga taacaggatg tccaacaggt atagctcata
     6841 cttttatggc aaaaaaagca ttagaggaag cagggcaaga acttggatat gaagttaaaa
     6901 tagaaactca tggagctata ggtgtagaaa ataagttgac taaagaagat ataaaaagag
     6961 ctgaatacgt aataatagct tgtgataaag aagttcaact gagtagattt ataggaaaga
     7021 aagtgctaca aaagaaagta gtagatgcta agaatgtttc attagctaaa aatttaatta
     7081 taaacgctga aaatggaatg gctaaagaaa ttaaaccagg tggaaagact ttagaagatt
     7141 caaaagaaat gcttcaaggg tcagagaaaa agggtgttta taagcactta atgagtggtg
     7201 tatctgaaat gattccattt gtagtgatag gtggaattgg tatagccata gcttttatgt
     7261 ttggtatatt tgcatcagac ccaactcatg aaacatacaa ccctatagct ggattctttg
     7321 gacaacttgg aggcgattca gcatttaagt tatttatacc gatacttgct ggttttatag
     7381 ctaaatcaat agcaggaaga caaggatttg ctcctgctat gatagctggt tttatggcag
     7441 ttattggagg ttcaggtttt ataggtggta tggtagctgg ttttgcaaca ggatatatag
     7501 ctttaggagt aatgaagata acagaaaatt taccagatgt attaaaagga gtaaatgctg
     7561 tattgatatg tccacttttg attgcattta cgagtggagc tataatgttc tttgtagtta
     7621 atacgccagc atcatggtta aatgaagctt tacaatcttg gttacaaagt atgtctggaa
     7681 ctaataaagt attattaggt gcaatattag cagggatgat ggcatcagat atgggaggac
     7741 ctattaataa aacagcttca gcttttggat tggctatgtt ttcaagtaat atatttgaac
     7801 catcagcagc attaatggta ggaggaatgg tacctccatt agcaatatca ttagctatgt
     7861 taatattcaa aaataagttt actaaagaag aaagaaagtc atctctacca actgctataa
     7921 tgggggcatc tttcataaca gaaggcgcaa taccgtttgc agctgcagac ccattaagaa
     7981 tgattccagc aaatataatt ggttcttgta taggtggggc aatatgtatg agtttagata
     8041 taactttaat ggcaccacat gggggaatat ttgtaatacc atttgcatgt agtgcaccag
     8101 ttgcatatat agcttgtata ttgattggta cagtaataac agcattgatt ataggggtaa
     8161 caaagcctac tataaggagt gaaaatagtg aagttgaatt acttggtgct taaattataa
     8221 ataatagtaa aaataattaa tggaataaca acaagtatat agaataataa taaggatatt
     8281 tagaatcagt taactatttc taaatatcct taatttttat tttgaaatta tattatctat
     8341 tatatcaatt tagtaaggtg tatcataaat tcttttatga tacagaagta tcttagtaat
     8401 tttacatagt tagtaaatat gttaaaatag tacttaatat aaacatagaa atatataaac
     8461 ataggtaggg agggctaagt atgcttgtgt cgaggcaaat tgaatttctt aaattgatac
     8521 taaacgaaaa taaatataga cctataaaat actttaaaga taaattaaat gtatctgata
     8581 aaactttaca aaaagatttg aagatgattg agaaatattt agaaactttt aatataaaaa
     8641 ttgatataaa aagaggctat ggaatattaa tagaagattt agcaaagaag aacatagact
     8701 taattaatag tttaaatata caatcgaagg aaaataaatc tttatcagta gagcagagaa
     8761 gaatggagat tttaaagtat ttattgctaa attcaaataa tataacttca ataaatcagt
     8821 tagcagataa atattatgta agtaaaactt ctatagtaaa tgattttaaa tatatagagg
     8881 catggataaa agaatacaat ttaaaactta ataaaacttt agaaggaact aagattatag
     8941 gtaaagaagt ggatataagg aaatcgattg ccaaaatgat ggatgattta ttagatgaaa
     9001 attatgaaca acatgatata tctgagttaa caagattaga ttctgctaca tttagtgcat
     9061 tgataaattt atttgatatt gattctatta tatttgtaga aactataatt acagaattag
     9121 aatacaattt aggatatact ataaatcagc cctactatat aaatttaatt acccatatac
     9181 ttatatgttt aaagagaatc gaagaaggta atcaaataga atctcaggaa gaaagagaaa
     9241 tttctgtaga taatttggac gagttagtat ataaaaatgt cactacactt atagataaga
     9301 tagaaaatag atacagagta aaaataacga aagatgaaat tatgtacata tatatttttt
     9361 tggtatcatc tggatttagt agcgaaaata atagtagtta caatgatgaa tcagatagat
     9421 taagtgaaag tgaaaaagtt tcatctataa tgatagagaa tatgtcacga tttttaaata
     9481 taaatctcaa aaatgatgac ttactacaga aaagtctagc atctcatgta aggcctatgt
     9541 taaacagatt aagatatgat atccaaataa aaaacccgtt gttaggagag atacaagagc
     9601 gattttcaga tgtgctggga ttatgtatga tgactataaa tattatgaca gagtatgata
     9661 atctgaagaa tattagtata gatgaagttt cgtatcttgc cacatatttt caagcggcaa
     9721 tagaaagaaa tatgagtagt aaaagagtaa tagtagtttg tcatacagga tatggaacat
     9781 ctcagctcct tgcagccaga ttaaaaagag aatttcctga atggactata gttgatatag
     9841 ttcctatgca tcagttgagt aagcgaaatt tagatgatgt agactttgta atatctactg
     9901 taaacttaga tttgaaagat aaattacata tagttgtatc agtactatta atggaaagtg
     9961 atattaataa tataaaaaat gctttacttt ctaaacctaa aaaaagtatg aacttagata
    10021 caaacttatt aggaatgtat ttagaagata atatatattt taattttgat gtagctcaga
    10081 tggaaagttt aataggtgtg aatttaggga gtaaatatga atctatttca ttaggaaaag
    10141 atttaaatat atatatacat aagcaaagta aattaaataa agggataatg aatatcaata
    10201 atgttactag aacgattgat atttatttag aaattagtaa tcaaagtttt ttaaagaata
    10261 ttttaatgga aatatcaaat ttatataggc aaaagaacta tataaactat ataatagatt
    10321 gcaagagcaa agaggatatt aaaatattat ttaagaataa tctattggag aaaacaaatg
    10381 gaaattagag aaatattaac agaagaaagc attattttag atttgaaatc aactacaaaa
    10441 gaaggcataa tacaggaatt agtagataaa ttatacgata caggaagtat aactttaaaa
    10501 agtaagttta taaaggcatt aaaacataga gaaaaaatag gctctactag tataggttat
    10561 caaatggcca tgccacatgg aaaaacaaaa tatgtaaaga aggcatccat agtaataggt
    10621 aaatctaaaa aaggtgtaga ttggccaagt atagatggct tacctgtaaa ggtaataatt
    10681 atgtttgcta tgccacaatc tggaggtaat gaagatatga tattaatatc taaaatagct
    10741 tacattttag gtggtaaaaa tataggtgaa agacttttaa atgcacatac aaaaaaagaa
    10801 atattagata tattttatga agtgtaggta ttgtataaat tataaatttg aggtttttaa
    10861 aataaaatgt gtattctttt aaatagtata gattaatttt tagacactag agaggagagg
    10921 atatatatgg caatatattt agaaatgcct aaattaatac atgaatttcc ttttcgtact
    10981 ctatcaaatg aaggagaagt attaacaaca ccacactggc ataaggaaat agaaatatta
    11041 cttataacag aaggtgtagt aaatctattt ataaatgaca agcctatgca attaaaaaaa
    11101 ggcgagatag tgataataaa aggaggagat attcattata tactgccatc accgggaagt
    11161 gaaagattag tatttcaatt tgatatatct ttttttaatg atttaatttt attaaatgag
    11221 gagaaagaaa gcttaattaa tttattcagt agtataaaaa aatgtagtag agattggtca
    11281 gcaaaaataa gaaagtcagt tttcgatata ttgatagata tctacaatga agacctatac
    11341 aaaatagaag gatatagtta tgcaataaaa ggtaaaatgt ataatttaat tcctatatta
    11401 tatagacaga ttcctagaga aattgaagaa aatacagtag aatatgaaat aaactctaaa
    11461 gagatattag agagattgaa caatatattc aaatatgtag aaaaaaatta taaacaacct
    11521 ataaaattag aagatgtgtc ttatgaagta ggatttagtg tatattattt tactaaattt
    11581 ttcaaaaaaa atacaggaaa aacatttata acttttttaa atgaatatag attagaaaaa
    11641 gcaaagtgga tattacttaa taacaatata tccattgtag atttggttga agaagttggt
    11701 tttggaagta caaaaacttt ttacagagta tttaaagaaa aaattggagt ctctccatta
    11761 gaatttaaaa aaagatataa tagcttggag aaaaaaggga gtgtctcaaa atgaacttag
    11821 ttcatgaggc actttctttt atatgaaatc aaatatattt tcagtatttc aacaatgaaa
    11881 attccattca tttataaagt ctaaatttta gttctcaata caacctctat ttcgaaataa
    11941 atccttttat atatataaat atacagcaat atatgagaat aagataacaa taatgaggat
    12001 ataaaaatgt atttgctata tatataatta tatacataga tatctaatat tgatatttaa
    12061 tatgaaagta ttgaaaatag acaatattaa ttatagtgag taaaaaatat aattggattt
    12121 ttaaattgaa ttatttaaaa ggtatagtat gaaaaaaatc ttataactaa atatgagata
    12181 gaaattataa aattttatac tgtgaaagag gtagatgcat gaaattaggg aaagattttt
    12241 tatggggagg ggcaatttca gccaatcaaa gtgaaggatt ttatttagat ggaggaaaag
    12301 gtttatctca gtttgataaa ctcccattaa atgataagag attaaaaaat gtatgtttag
    12361 atgaggataa tattttaaat gaaaataatc aatactatcc aagtcatata gggattaaat
    12421 tttgtgaaaa ttacaaagaa gatatagcaa tgttagcaga aataggtttt aaatgtttta
    12481 gattttctat atcttggtca agaatattcc caacaggaga agaaagtgaa ccaaatgaaa
    12541 agggattaca actttatgat aatataataa aagaattaaa aaaatataat atagagccat
    12601 taataacgat aagccacttt gatatacctt attctctagt tgaaaaatat tctgggtggg
    12661 ctgatagaag agtggttaat ttatatgtaa aatatgctga aattttgatg aaaagatata
    12721 aagatgatgt caaatactgg attcctttta acgaaatgaa tatgataatg catattccat
    12781 atataggtgc tgggttaact tttatagctg atgaaaatca acttcagaaa aaatatcaag
    12841 cagcacacca tcaattatta gctaatgcaa aaactataga aataggaagg tatataaata
    12901 aagaatttaa gtttgggtgt atgttagctg ctggaaaaac atatccatac acatgtaaac
    12961 cagaagatgt atttgcaact ttagagcatg agaggcataa tttgtttttt tctgatgttc
    13021 aagttagggg tgtgtatcca agctttatga gttattattt aaaatcaaat caaataaaat
    13081 taataataaa agaggaagat ttatcaacac ttaaggataa tactgtagat tttgtatctt
    13141 ttagctatta ttctagtgct tgtagttcag ctagagctga aggtttagaa aaatcaaagg
    13201 caaacggacc agaaaccata aaaaatccat atcttccaaa atcaaatagt gtatggcaaa
    13261 acgaccctgt agggttaaga ataactatga atcagttata tgaaaggtat cagttacctt
    13321 tatttatagt agaaaatgga ctgggtactc aagatattgt aaatgaagat ggaaaaataa
    13381 ttgatgatta tcgtatagat tacatgaaag aacatattaa aaatatgatt cttggagtaa
    13441 atgaagatgg aatagagctt ataggatatc ttatgtgggg atgtatagat gtaaccagtg
    13501 taagtgaagg aaagatgaca aaacgatatg gcataattca tgttgattct gatgactatg
    13561 gaagaggctc atttaaaaga actaaaaaac aaagtgcttt ttggtataaa aaagtaatag
    13621 aaacagatgg agaaatttta tttaatgata tggaaactca tgcataattc attttaagaa
    13681 aggagtgtta ttatgagttt aatatttact actaaggacc aacttgtaga gctagggttt
    13741 agtaaggata caataaaatc caatgaaacg ttgtttacac tagctaatgg acatttagga
    13801 gttagaggga atattgaaga gtctgatttt aataatgagt atgtagataa tatgggtaca
    13861 tatgtaaatg gattttatga agaatctcca attacatatg gagaaagcgc ttatggctat
    13921 gcaaaacaaa atcagactat ctgtaagtta cctaatgctc atatagtaaa tttctctatt
    13981 gaaggagatt gctttgattt atcaaaaggt attacaagtg aacatactag gatactagat
    14041 ttaaatgaag ggattcttaa acgttcattt atatgggaaa actctcgtgg tagaaaagtt
    14101 aaagtatcta tagaaagatt agtatcacaa gatatagaag aattaatgat gatttcatat
    14161 acaataacac cattgaattt taatggaaaa ataaaattta aaaataaaat ggaaaatagt
    14221 atggagaagt ttaattgtga aaaagtagat gacccaagag tggcatctat aaataagaag
    14281 caatttaaaa ttaatgtaaa aaaatataac aataagtatt ttatgaattt agttacagaa
    14341 agaagtagat tagggttatg gtgtgggttt gattcatata ttacttgtgg aaatattaca
    14401 aatgagacct ataatgtttc agatgaatgt attgagcgag aaatagaagt ctatgccaaa
    14461 aaagatgaaa ggttaaattt agaaattgtt gtaggctata gtagagtata ttctgattta
    14521 aatctaaatg aagaacgttt aaaatcatta gaatgtctgg agtcgttaaa atgtttagaa
    14581 tcagaagcac atttaaaatc attagaattg gttttaagag aagcatgtaa tattggattt
    14641 gataaagtca aaaatattca gagaaagaaa atgaatgagt tttggacaac ctctgatgta
    14701 gagattgaag gcgataaaac tcttcaactg gggataaaaa ccaatttatt tcatatttat
    14761 caatcagcag gaagagatgg aaagactaat atatcagcaa aaggtcttac aggtgatggc
    14821 tatgaggggc attatttctg ggatacagaa atgtatatat taccattttt tatatatact
    14881 caaccatcca ttgctagagc tttattagat tatagatact caatcttaga tgcatcaaga
    14941 gaaagagcta gagaacttgg aataaataaa ggttgcttat attcatggag aactataaat
    15001 ggtaatgaat gcagtgcata ttatccagca ggaacagcac agtttcatat aaatgcagat
    15061 atagcttatg gtgttagaac gtattttgaa gcaactaatg atgaagaatt tatgatgcaa
    15121 aatggcttag aaattcttat agaaacatca agattttggg ttgaatttgg agattatata
    15181 gaatcaaaaa ataataaatt ctgtataaat ggagtaacag gaccagatga atatacagca
    15241 atagtaaaca ataattacta cactaattta atggctaagt ataatttaat atatgcagta
    15301 cagatggtag ataaatttaa gcataattta ttttcaaatg aagtatttag aaaagttaaa
    15361 ttacaagaat atgaagtgaa aaactggata aatgccgctc aaaatatgta tttaccatat
    15421 gatgaagaga agaaaataag caaacaagac gattcatttt tagaaaaatc agtatgggat
    15481 tttaaaaata caccaaaaga aaaatatcca ttacttctcc attatcatcc attaacaata
    15541 tatagatatc aagtaaataa acaagcagat actgtacttt cagcgttgct tttccctgat
    15601 gagtttagct tagaacaaaa gaagagagat tttgaatact atgaaaaaat aactactcat
    15661 gattcatctt tgtcaaggtc aatatttgga atgatggcaa gtgaaattgg aaatcatgaa
    15721 aaagcttata actattttat ggacacagca ttaatggata ttaataacct acaaaaaaat
    15781 acaagtgatg gaatacatgc tgctaatatg ggtggaacat ggatgagttt agtatatgga
    15841 tttgcaggaa tgaaaataaa aaatgataaa ttaagttttg aaccaaggat acctaaacac
    15901 tggaaaaata ttaaatttaa tatattattt agagataatt tagtttttgt aaatatagaa
    15961 gaatataaat cttattataa gttaatcaaa ggtagaaata taaccatatt acatgataat
    16021 aaagaaataa aattatcaaa gaacataata gaagttaata attgttttaa agggagtaat
    16081 taatatgaaa gcagtcttat ttgatcttga tggagttata acagatactg ctaagtatca
    16141 ttttgaagct tggcatgaga tagcaaaatc gctagatata gaaatagatg aaacttttaa
    16201 tgaaaaatta aaaggcataa acagggaaga atcattagat aaaatattgt tatctgctaa
    16261 taaagtagat agttatacta aagaggaaaa gttaaaggtg tgtgaagaaa aaaataacat
    16321 ttatataaag ttaataaact cattaacata taaagatata ttaccaggga taaatgagct
    16381 tataaaagaa cttaaaaaaa ataatgtgaa attgggaata gcatctgcta gtaagaatgc
    16441 acctaggata ttagaatctt tgggagtaaa agatgaattt gattgtatag taaatccatc
    16501 tttattaaaa aggggaaaac catatcctga tatttttatt gaagggagca agatgctaaa
    16561 tttaaaacaa tatgattgtg taggaataga agattcttat tcgggtatta aagctataaa
    16621 tgctgccaat atgacatcaa ttggtgtggg gaaaaaagaa gtactaggaa aatgtgacca
    16681 tattgtagaa gatactagct tattaaatta caatacacta ttacaagctt ggagtaaaaa
    16741 gattaatttg gattaaaatt atattaaaaa ttattttaaa agagaggata aggtatgaag
    16801 aataaatatg aagatgtagc tcaattgata attgatggac ttggaggaaa agataatata
    16861 attgacttaa ctcattgtat gacacgatta aggttcatct taaaagatga aaagaaagtc
    16921 aatgcagata aattaaaatc tatagataag gtagttggag taaactcaac ttcaactcaa
    16981 tatcaggtca taataggtaa tgaggtaggt gttgtattta atgcaataat aaataagggg
    17041 ataaatacca caggagatat taaaagcgat ataaaaaata aaaataaaaa aaatagtatt
    17101 ttaaataata taatagatac aataactggt tgtatgacac ctatgatacc agcccttaca
    17161 gcatctggga tgataaaagt tatattatct ctttgcacca cttttaattg gctatcatca
    17221 aattcaagta catatagagt attagatata ataggagatg tagcatttta ctttatgcct
    17281 ataatacttg caataaatgc agctaaaaaa tttaatgtta atacatcaat agcagttata
    17341 gtagtaggtg tgtttttaca tccaaatttc tctgcatggg tatcaagtgg ggaccctata
    17401 tcatttatag gagtaccaat tcaaggtgtt atatatgcag catctgttat ccctgcactt
    17461 ttaacagtat ggatgatgtc atatatagaa aatttcattg ataaattaac acctagcatg
    17521 ttaaaaacaa ttttaaatcc aactttagta ttattaataa gtgcgccaat agcattaata
    17581 gtaattggac caataggaaa tttcttagga gagggattag ctagtataat aaatttacta
    17641 caaggaaggt taggatttat aatggtttgt ttattagcag ctgcaatgcc atttatcgtt
    17701 atgacaggga tgcatcatgc attgactcct atattcatat cagcatttgc aactacagga
    17761 caagaatcgc taatattaat atctcaagtt tgtgctaatt tagcgcaagg tggcgcaaca
    17821 ttagctgtag cattaagaag caaagatagt aagatgaaac agttagcatc agctgcatgg
    17881 atatcagcta ttatgggtat aactgaacca gctctttatg gagttacatt gaaacttaaa
    17941 aaacctgcta tgttagcatc tataggagca ggaatcgcag ggttatttgc aggaattact
    18001 catgtgactt tatatgttcc acaaaatagt ataatggcag tacttggatt atcaggggaa
    18061 aaggggattt caaatatagt aaatggactt atagttatat ttatagcatt ggttgctcca
    18121 tttattatga catatgtttt tggagttgaa aaaaatgtta aataaagtac agataatgta
    18181 gtagaggaag tataatatta actatttaca ttaatagaat ttttattatt attgactata
    18241 acttgttttg tatttatatt gatgcaaaga aataagttaa tattaataag aataattatt
    18301 ataactgaca aaaagcaaca taaaagattg atgaagtata tggaaataaa aagttgacaa
    18361 aatatgtctg atgttttatc atattaattg gaattaggta gtaattgttt aagtacaagc
    18421 taagcttatt cattaataat agcagtatcc taggaaggag tatgcccatt attatggata
    18481 agcttttttt atgcttaaaa ggaggataat catgataatt aataagatat taaacaataa
    18541 tgttgtaatc acattagatg ataatgatga agaagttatc gttatgggta aaggaattgg
    18601 ttatcaaaaa tcaaaaggca atctaataga taaaactaaa gtaaataaag tatttagaat
    18661 atcaaataaa gaaatatcaa acaaactaca agaattactc aataatattc ctatagaaca
    18721 catgaaatta tcaagtgaaa ttatagaata cgcacaaata aagttaaaca agaagctaaa
    18781 tgaaagtatt tacatatcct taagtgacca tacatattct gcaattcaaa gaatgaaaga
    18841 aggaataaat gtaaaaaatg caatcctttg ggaaattaaa agattttata aagaagagtt
    18901 tgaaattgga atgaaagcac tagatattat tgaaaacaaa acaggtataa aattaccaga
    18961 agatgaggct ggatttatag cctttcatat agtgaatgct cagttaagtg aaggtcatac
    19021 tttggcatct gatattacga agttaataca agaagtactt agcatagtta ggtatcattt
    19081 taggatagaa tttcatgaag aatcagtgtt ttattataga tttataatgc atttaaagtt
    19141 ttttgcgcaa agattgcttt tagatagtgc acatgaaggt gagacagaca aagaattact
    19201 aagcattatt aaatctaaat ataataaaga gtttgaatgt gtagttaaaa ttaaaaattt
    19261 cattaaaaaa caatataact acatattaac agatgatgag cttatatatt taacaataca
    19321 tttagctaaa gtagttaaag actcaaatat gtaagtgaaa taatttttca gcagtatatt
    19381 aagaatgatt gaaaggagtg atactaacaa aattatactt atggttggat ggtaacattg
    19441 agttggccaa accttcaaat ttcaaaaaat taatttaaac gaaatatgga ggagaaaaat
    19501 gggaaaatac aaacaattag cacaagagat agtaaacaat gttggtggaa aagaaaatat
    19561 tagtggatta gtacactgta ttacaagatt acgttttact ttaaaagatg aaagtatagc
    19621 aaatgacaat gtattaaaaa acatggaagg tattgttact gtaatgaaaa gtggaggaca
    19681 atatcaagta gttataggaa atcatgttga agctgtctat aaagatgtag ttgaaataat
    19741 tggtttagat gattcaagta caagttcaga aactaaaaaa tcaggtaata tactagataa
    19801 aggtatagat ataatatcag gaatttttca gccagtgtta ggaataatgg cagcttgtgg
    19861 tatgttgaaa ggatttaatg cattatttgt agcaatggga ttatatagtg atgtatctgg
    19921 tggttatatg gtaataaatg cagcgggaga tgcattattt acattccttc ctttgttctt
    19981 aggatatact tcagctaaga agtttggatt aaaaccaatg ttgggattag cactaggagc
    20041 tgcagtatgt tatcctgcta tacaaggtag cagtatatca gcaggagcag atgtgatgta
    20101 cactttattt aaaggaacaa tgtttgcatc accagtatat attgatttct ttggactacc
    20161 aattgtatca attgattata ctggaacagt cgtaccaatt atatttatag tgtattttgc
    20221 ttctaggtgt gaaaaattat ttaataagtg tgttccaaat ttagttaagt ttttctttgt
    20281 gccaatgctt acattattaa taacagtgcc tgttgcactt atagttatag gccctatagc
    20341 aacatttggt tctacactta tttcacaaat tgtaatttca ataagagatt ttagtccact
    20401 attggcaggt gctatagtag gatttacttg gcaaattctt gttatttttg gtatgcactg
    20461 gggatttatt cctgtatata taaacaatgt tatgacttta ggatatgata atgttatgat
    20521 gccattcttt gcctgtacat ttgcaagttc ggcagttgtg ttggcaatat ttttcaagac
    20581 taaagataaa aaactaaaag agatggcaat accaaacttt atatctggta tatttggagt
    20641 tacagagcca gcaatttatg gaatattatt gcctttaaag aaaccattta taataagttg
    20701 tattgctagt ggtattggag gcgctttcta tggattcttt aatcttagaa aatttataac
    20761 tggaggaatg ggaatatttg aacttccagc aatgattgaa ccagatgggg gaatgggtaa
    20821 cttaatagta gctctttctg gtatagctat atcaatggtc gtagcttttg tattaactat
    20881 gattttatat aaagatgaga aagtagaaga gtctaaaaaa gtaagaaata agggtaaaga
    20941 agaaataaaa gaagtcaagt ctactaaatt agaaagagaa atagtaacaa gtcctataaa
    21001 aggtgaagta ttaaaattaa gtgatataga ggatgcagca tttgcatctg gtgtactagg
    21061 tcaaggggtg gcaataatac ctagtgatgg aaaagtagtt gctccagtag atggggtagt
    21121 tacaacctta tttcctagtc tacatgctat agggattctt tctgatacag gtgttgaagt
    21181 gcttattcat attggattaa atactattca attagaggga agaggttttg aggcatgtat
    21241 aaaacaaggt gatagaatta caaaaggtca aactatatta aactttgatg ttgatgcaat
    21301 aaaagagttg gggtatagta ctgtaactcc aattgttatt acaaattcat cacaattttt
    21361 agatgtagta gaaacagaaa gtaaaaatat tgaattagaa gacaatctaa ttacagtctt
    21421 attttagtaa agattgaggt attagcataa tctagtgctt tggttataat tttaggaggc
    21481 tttgactatg agttttaaga atgatttttt atggggagga gctactgcgg ccaatcaatg
    21541 tgaaggtgga tacaatgaag acgaaagagg attggcaaat gtagatgtat gtccaacagg
    21601 aaaagataga acagcagtta ttacagggaa actaaaaatg tttgattttg atgatgagca
    21661 ttattatcca gctaaaactg gtattgatat gtatcataat tataaagaag atataaagtt
    21721 atttgctgaa atgggattta aagtatatag aatgagcatt gcatggagtc gaatatttcc
    21781 aaaaggtgat gaagaaacac caaatgaaaa gggacttcaa ttttatgaaa atatatttaa
    21841 ggagtgtaaa aaatataaaa tagagccttt agtcactatt acacactttg attgtccaat
    21901 gcatttagtt aaaaagtatg gtgcatggcg taatcgaaaa atgataggtt tctatgagaa
    21961 attatgcaat gttatcttta gaagatataa aggattagtg aagtactggc taactttcaa
    22021 tgaaataaat atgattttac atgctccatt tatgggtgct ggtatttgct ttgaagaagg
    22081 tgaagatgtt gaaaagataa aatatcaagc tgcccatcat gagcttgtag ctagtgcaat
    22141 tgcaactaaa atagcacatg aagttgattc aaataactta attggatgta tgtttgcagc
    22201 tggaagtgta tatccttata gttgtaatcc taatgatgta tgggaagcta caaagttaga
    22261 tagagaaaat tacttctttg tggatgtaca atctaaaggt aaatatccta actatgcgtt
    22321 aaagtatatg gagcaaaaag gtattacacc agaaatggaa cctggtgata ttgaattatt
    22381 gaataaatat acagtagatt ttatttcatt ttcatattac aatagccgtt gtgttagaac
    22441 cgatgaaaat gcagatgata tggctgaagg aaacatattt acatctgcta aaaatccata
    22501 tctttcatac agtcaatggg gatggccaat tgacccatta ggtttgagaa ttacactaaa
    22561 tcatgtatat gatagatatg aaaagccact atttgttgta gaaaatggat taggtgctaa
    22621 agatatagct gatgaaaatg gttatgtaga agatgattac agaattgatt atataagaga
    22681 acatataaaa gctatgcatg atgcagtttc tatagatggg gtcgatttat taggttatac
    22741 tacatgggca cctattgatt tagtaagtgc tggtacaggt gaaatggaaa agcgttatgg
    22801 atttatatat gtagatagag acaatagtgg taatggaagt ctaagaagaa tgaaaaagaa
    22861 aagttttgaa tggtataaga aagtaattgc ttcaaatggt gaagatttat attagacaag
    22921 aaatctatgt taaataagaa atttgtatta aataagaaga gtataaaaat taataataag
    22981 tataattttt aactttttta actatagaaa atcaatacag aattatttta cattgtattt
    23041 atgcaatagt atgattcaca aataaaattt tagaaaataa aattgttaag aagtatggga
    23101 agtaagtgca atttactttg taataaaaga tgaaaaaggc aagcttaggt tttaagcttg
    23161 tcttttttat tatgtataaa ctatgatact ttaaaattct atgaaaaatt tatttaatag
    23221 agaaattaaa aatttaatgg taatcaaatt ctttaccaat gaatataaaa aatgtctacg
    23281 tgttataata gtttaaacta ataaacgaaa ggtagatgaa tgaatatgaa ataccaacat
    23341 ataatagaag ttgacaaaga actttgcatt ggatgtgggc tttgtaaaaa tgattgtcca
    23401 gtaaacaata taattataga aaataaaaag tcagtaatta aaaaacaaga ttgtctaatg
    23461 tgtggacatt gtgcagcaat atgtcccaca aaagctatta ccttgacagg atttgatgaa
    23521 cctcctatag aacttacaaa taagccaaag ctagattcag atgaattact aatggcaatt
    23581 aaatctagaa gaagtatacg taaatttaaa gacaaagaag tttcttcaga aattataaag
    23641 caaattattg aggcaggtag gtatacccct agtgctaaaa attcacaaga tgtatcatat
    23701 attgttttag acaataaaaa gtcaatttat gaaaacgaag cagtgaaatt ttttagaaaa
    23761 ataaaaccta ttgcaaatat agctattaaa tattcaaaag aagttgagat tgacgataac
    23821 tttttcttta agcatgctcc tattgcaata atgataatta caaaagataa gattagtggt
    23881 tcacttgcag catcaaatat ggcattaatg gcagagtctt atggcctagg tgttttattt
    23941 agtggatatt tttcagatgt agcaaataat tcgccaaaat taaaaaagct tttaggtcta
    24001 aaacgcagta atcatgttct tacaacatta gtaattggat acccagatgt taaatataga
    24061 cgtactgcac agaaagaagt agcaactgtt agatacttat aagagagggt ggattttatg
    24121 gaaaaagaat gtcaacaaag aatgcaagaa ataacgtcta aatttaaaga atgtcgcaaa
    24181 gctatctctg ctatgggtga tgagactaga caattgattt taattgctct cttagagagt
    24241 gattttaatg gtatacgagt gggagaaatc acaaaaaaaa cacatctttc tcgtcctgct
    24301 gtatctcatc atcttaagat attaaaagaa gcagagattg ttaatttgcg tagagaagga
    24361 acaaaaaatt attattatct tgattcaaaa aaatcacaat ggaggtcact aacagagcta
    24421 ataaatctta tatttgttgg aatacaacat attagtgaaa atgatataag aaaaggtgat
    24481 taaattatac ttgatttaat acaaaaagat tgatttagga actaaaaaga actaaaaagt
    24541 tcactttgag acactatctt ttgaactata cccatcttag gagacagtta aaatatgtaa
    24601 aactgtataa aacaatgggt atagttcatt ttataatggt ttaaatatta agaatatatt
    24661 attctaaata gtatctttct ctgttttgag ttttattttt atactcaata gcttcagtgg
    24721 ggcaaccaca aatacatgcc atacaatgag tacagttatt tttccaaact ggttttttgt
    24781 tttttaaatt aatattattt aaaggacata gctctacaca ttttccacag ccaatgcatt
    24841 tatcagtata atgaaatcct ttggcactaa taattgtctt ataaaaaact gtatttatta
    24901 taccactttt aattgtacct cctaagccac taggagtaat aatttcaaat tcattttcat
    24961 ttcgtatatc agaaataatc ctatatattt gtttatctgc ttctttaatc atttgttttg
    25021 atgtttcttt gtctgttgta gaaaataaag ctatataatt ctctggcatt ttaatttctg
    25081 ccataccttt taattgccat tccttgtatt tacatagttt ttgaatatag tttatggatt
    25141 ttgctgtatc tccaccacaa gtcattacaa agtaaactct gttgcttcct agaaattttg
    25201 tttcttttat aaaatccgta accactattg gcaatctcca tgcataagta ggacatacaa
    25261 atatgaatgg tttatctgtt gatattaatt catctgtttt ttcctctttt ataagttgat
    25321 ttagtgatat cagttcatca tttaattctt gtgcaatttt acgagccaca tatcggctat
    25381 taccagttcc gctaaaatat aaaatcataa taatatctcc ctaactattt tttattttaa
    25441 ctcttaattt tttatttatt atagtaattt attgatatat tctattcaaa tgtatgtaaa
    25501 atttagtagt taagaaaatt ataacacatt agtaattttt atacttattg gtaaagaatt
    25561 tgattaccat taaatttagc aacaagacat caactggact tgtatatgag aagggataat
    25621 aatttgctta tgaaagatta taaatataaa ggttattttg aaggtgtaga aatatttgat
    25681 aatgtaaatg accttaactt aaatacttta acaacagtta gtagatttgt gtttgttttt
    25741 ttgtttacga taacttaagg aaatatgtta cctgaacaat aaatttatat aataaaagta
    25801 tataaatata tatatgaatg ttaagattag ttgatagaat ttaaataata aatccaatat
    25861 tataaacata ggaggtatga aaaatatgat taatgagaat ttaaaaagtc taagaaaaat
    25921 caatcagtat acacaagaag aattagctga aaaacttaat gtttctagac aatcaatagc
    25981 aaaatgggaa agtggagaat ctattcctga tataggtagt tgcattaaac ttgcaaaatt
    26041 gtacaatgtt aaactagatg acttagttaa tcattcggaa gaaaaaacag gtattatagt
    26101 acctcctaaa ggaaagtttt tctttggagc agttgtagtt ggtgaacgtg gtcagattgt
    26161 cattccaaaa gaagcaagag aagtatttaa tataaatgca ggagataaat tattggtact
    26221 tggagatgag gaaagaggat taggaattgt tcatcaacgt gatttgataa attttatagg
    26281 tcatgtggga gtagtacata aagaagaatg atttaaaaaa attataaaag gaggtatgaa
    26341 atatgagtaa aatagtattt tttagcattc ctgcatatgg tcatactaat cctactattg
    26401 aagtagttcg tgaattagtg gatagaggaa atgaggtatt gtattattct tttaatgaat
    26461 ttaaagataa aattgaaggt gctggagcaa aatttatttg ttgtgataaa taccttccag
    26521 aattactccc tggagatgag aaaaaaattg gtaaggattt tcctggatta attgagatga
    26581 tagtagatac tactatttct ttagatgaaa aagtttgtag agaacttaaa gattttaatc
    26641 cagattgcat tgtatcagat tcattaagtt tttggggaaa gttatttgca caaaaactta
    26701 atattccata tgtatgctct acaacaactt ttgcatttaa taaacataca gcaaagttga
    26761 tgaaacaaaa ctttatagaa attatacgta tgatttttgg tgtaagacgc attaataaaa
    26821 aaataaagct tttacaaaga aaagggtatg aagtaaaaaa ttttatttct atagtttcta
    26881 atgataatga tacggatacc attgtatata catcaaaaga atttcaacca atggtagaaa
    26941 ccttctctag taagtattca tttgtaggtc catcagtatc aaagttgatt attgaaccta
    27001 aagagagaaa aagaaagcta atatatattt cgcttggtac aattaataat aaaaatgtta
    27061 gcttttataa aaactgtata aatgcattta aagattctga tgttgatgtg attatgtctg
    27121 ttggtagaag tactgatatt aaaagtttag gaaatatacc aaataatttt gaggttaaaa
    27181 atagtgttga acagattgct atacttcaaa aaactgatgt gtttgttact cattctggga
    27241 tgaatagtgt taatgaaagt ttatattatg gagtacctat ggtattattt cctcaacatt
    27301 cggagcagag aatggtagct aagagagttg ttcatttggg agcaggaatt atgttaaaag
    27361 aggataaatc agaaagtatt aaaaaagcag tatttcaagt tataaatgac aatgaatata
    27421 aagaaaatgc aactaaacta tcaaaaagct tttataatgc aggaggctca aaaaaagcag
    27481 cagatgtaat attacagatt atatgtaata gtagacaagt tgacatataa tagatttgtt
    27541 aaaaatatat atacatacat gtaatttaat caaagtactg acagtggggt cttttaacct
    27601 accagttggg gcatatatat tgatgctaaa tatgagggat gttatatttt aataaaaaag
    27661 ataacatcct ttttttagtg cattatatcg agttttaaat aataaatgaa ggaggattaa
    27721 tatgaaaaaa tataaagtta tttttatagt attaattata ggtattataa gtgtaacagg
    27781 gtttgttgta tataaaaact gcattaaacc atggaaagat gctgaaacat atattgataa
    27841 gtatatggta aaacaagata tttcaaaaga taatataaaa agtattacaa aagaaaaagc
    27901 caagaaggca agttacgaag gaatactata caaagtgtat tacaaagatg accctcgata
    27961 tgagtatcaa tatttttata gtgataatta ttatgctcta tatgtaaata aagtaatgtt
    28021 acaaatttat gatatggaaa ataataataa acttttaaga agtaatgagg aattaaaaag
    28081 tgtaaagtat ccaccaatat atcttaaata taagccagat aaatgattta tgacaaaata
    28141 gaataatttt tattaaaatt tatttaaaaa tatcactaga
//
LOCUS       DAMJYX010000043        27422 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJYX010000043 DAMJYX010000000
VERSION     DAMJYX010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.043"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            262..2280
                     /locus_tag="PC479_001966"
     CDS             262..2280
                     /locus_tag="PC479_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2804513.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2785..4797
                     /locus_tag="PC479_001967"
     CDS             2785..4797
                     /locus_tag="PC479_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2804514.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            5039..6952
                     /locus_tag="PC479_001968"
     CDS             5039..6952
                     /locus_tag="PC479_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804515.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6945..9164
                     /locus_tag="PC479_001969"
     CDS             6945..9164
                     /locus_tag="PC479_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2804516.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9273..10103
                     /locus_tag="PC479_001970"
     CDS             9273..10103
                     /locus_tag="PC479_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2804517.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            10093..13560
                     /gene="addB"
                     /locus_tag="PC479_001971"
     CDS             10093..13560
                     /gene="addB"
                     /locus_tag="PC479_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2804518.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13560..17387
                     /gene="addA"
                     /locus_tag="PC479_001972"
     CDS             13560..17387
                     /gene="addA"
                     /locus_tag="PC479_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2804519.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17433..18644
                     /locus_tag="PC479_001973"
     CDS             17433..18644
                     /locus_tag="PC479_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2804520.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18631..22158
                     /locus_tag="PC479_001974"
     CDS             18631..22158
                     /locus_tag="PC479_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2804521.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22289..23542
                     /locus_tag="PC479_001975"
     CDS             22289..23542
                     /locus_tag="PC479_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2804522.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23691..24743
                     /gene="ytvI"
                     /locus_tag="PC479_001976"
     CDS             23691..24743
                     /gene="ytvI"
                     /locus_tag="PC479_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2804523.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            24833..26059
                     /gene="pepT"
                     /locus_tag="PC479_001977"
     CDS             24833..26059
                     /gene="pepT"
                     /locus_tag="PC479_001977"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2804524.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26266..27318
                     /locus_tag="PC479_001978"
     CDS             26266..27318
                     /locus_tag="PC479_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2804525.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11656 a         2682 c         4850 g         8234 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tataatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacatatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcaa ataaggtata taaaataggt
     1621 ggaggattga cttctaaggt aatgagctct atagcatcat cattatctaa acacaataca
     1681 actccaacag aaccaggaaa tagtgggggc aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttagagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2461 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2521 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gttttttgtg
     2581 gatttttatg tcaataaatg atatagtata gataattaaa tgtattaaat atatttaatt
     2641 tttttatttt ttgagtttta taagtttatt tttatatatt acttatatat tatttatatt
     2701 ttgggttaga tacactttga catattttca aaagttaaaa gattaaaaat aaagtagaaa
     2761 tttatgttaa gtaaggagat taatatgaga agaaatacaa aattattaac aacagggatt
     2821 ctttcaatgg caatcgtcgc acctacaatg gcatttgcta ctgaatctaa tgctatggaa
     2881 aataacgctg atttaaatat aaacttagag aaaaaaagta tcgttttagg tagcaaatca
     2941 aaagttagtg tcaaatttaa agaaaaacca gatgcagata gcattacatt aaagtataaa
     3001 tgctatgaca tgccattgaa tacaactcta aattacaatc aatcaactgg ggcatatgaa
     3061 ggaactatca attataacca agacccagaa tatctaaatg tttgggaact acaagggata
     3121 acaataaaca gcaaaaataa tcctaaaact ttaaacagac aagacctaga aaagctggga
     3181 ttaaatttaa aagactataa tgtaacacag gaatgtataa ttgaagatat aacttctaga
     3241 aaagatgtaa ataaatattt gagaaaaact tcttcaccta ttacagaact tacaggaagt
     3301 gatagatatg aaacagcagt taaaataagt aaagagggct ggaaaaatgg ttcagataag
     3361 gtagttataa taaatgggga tgtaagtata gatggcatta tatcaactcc actggcaacc
     3421 acatataatg caccaatact tttggttgaa aaaaacaatg tacctaatag tgtaaaatca
     3481 gaattaaagc gcctaaaccc taaagatata attataattg gagatgagaa tgctatttct
     3541 aaaactactg ctaatcaaat taaatcaact gtaaatgcta gtcaaacacg tttaaatggt
     3601 tctaatagat atgagacatc tttattgata gcaaaggaaa tagataaaaa tcatgatgtg
     3661 gaaaaagtat acataacaaa tgctaatggc ggagaagtgg atgcacttac tatagcagca
     3721 aaagcaggtc aagacaagca accaattata ttaactgata aagatagtat tacagacaat
     3781 acatataaat ggttaaagag tgaggattta caaaatgctt attttatagg tggtcctcaa
     3841 atgatatcaa caaatgttat aaataaggta aatggaataa ctaaagatag tgttactaat
     3901 aatagagtat acggagcaga tagacacgaa acaaatgcaa acgtaataaa aaaattctat
     3961 acagatgatg agttagaggc tgttttagta gctaaatcag atgtacttgt tgatgcttta
     4021 gcagcaggtc cattggctgc gaacctaaag tctccaatac ttataacacc aaagacgtat
     4081 gtatctgcat accataaaga taatttagaa gctaaatcag ctaataaggt atacaaaata
     4141 ggaggaggat tgacttctaa ggtaatgagc tctatagcat catcattatc taaacacaat
     4201 acgactccaa cagaaccagg aaatagtggg ggcaagacag ttatgattga cccagggcat
     4261 ggtggttcag cacctggaaa ttcatctgga ggaatgattg aaaaagatta caatttaaat
     4321 acttcacttg caacaactga atatttacgt tcaaagggat tcaatgtaat aatgacaaga
     4381 gacacagata agactttatc tcttggaaat agaactgctc tatctaattc attgaaacca
     4441 gatttattta caagtataca ttataatggc tcaactaata aacaaggtca tggtgtagaa
     4501 gtattttata agcttaaaga taaaaatgga gggactacta aaactgtagc taccaatata
     4561 ttaaatagaa ttttagagaa atttaaactt acaaatagag gtataaaaac aagagtactt
     4621 cctagtgatt ctacaaaaga ttatttatac gttttaagaa gtaatgatat gccagctgta
     4681 cttgtagaat gtgcattttt ggataatgaa aatgatatga gtttaataaa ctcatctgca
     4741 aaagtaaaag aaatgggtac acaaataggt aaaggaatag aagattcatt aaaataaatg
     4801 gaaataaaaa tatttaaaaa cttaatgtaa atttatagtt gtaaatttat actaaagtca
     4861 aaaaatgaaa tataagtgta tcaatagtaa gactgtatgt acatttgcac gtgtgaatgc
     4921 aagtatatat acagtctttt tttgtagtta aatgcaataa tttgaatgtg ttttgtaaaa
     4981 tacacaatat gatataatta gataatatac acttaattta attgaggaag gtgattatat
     5041 ggaatattta tttgaaaaaa gtagtttaaa agaatatgga aatgaagctt tagataaata
     5101 tagagaatta ttagataaaa ctggtaataa ttcaaggtgt attcttttgt tagtaccaaa
     5161 caataacaca aagatacgat atgaaaggtc tctaagatta aattatagtg aagaactgaa
     5221 aataacaaca tatataagtt ttgtgaagaa agaattagtt aaatactggc cactaataat
     5281 agaaaaatgt gatgaaataa gtacaaaggt tgtatctcca acatttatat caaatagttt
     5341 aagtgaatat ttaatagtgc aaaaagttaa ggaaaaaagg atacaagaag gatattttga
     5401 agatattaca ggaaccaata aatccatagc aaatagtata atgactaaca taaataagtc
     5461 tgcatttaat ttaattgatt ttgattttat tggagaaaaa atatattcat caaagaaaaa
     5521 taaagatagt atatatagat tttcatacac tcaaatggat gaaattatat catattatat
     5581 aaattcctta ctatcaaatt caattttaga taatgcatta agcatatatt tatacaatca
     5641 atacctactt aatgatgatt tttatttaaa aaacttattt acagaagtga gatatataat
     5701 tgtggatagc cttgaaagct caagtaatgc agaagttgat tttataacag aagcattgga
     5761 taatacatta caaagttata tatattttga ttatagtagg gattattctg tatttaataa
     5821 tgtggatata gactatataa atgagaaaat aatatcaaaa attaagccta aagaagaaaa
     5881 tattaaaaag aaggagatta gaatagaaga tttatactta ttacctgcaa atatagaatt
     5941 aaatcaatca agtcaattat ataatgaaat gttagattta ataagtgaaa aagttatatc
     6001 tcttatagaa gaagatgtat ctccaagaga tatagcaatt atatctccta taaacaatag
     6061 tatattggag catcaaatta gagattcttt gatagaaaaa aatatagatg tttttagtac
     6121 taagaaggat aaaaaagctg tggattatcc atatgggaat gcattggtag tggctacatg
     6181 tatattttat ggatatttag attttataaa agatgatgaa tacttaagct ttttagaaac
     6241 tcttttagaa ataaatagga taaaagcatt taagattttt aatgaaacaa gacatttaag
     6301 tattgataat atagatgaac atagtcagta tcgtgagatt ttacagtata ttgaagaaaa
     6361 aaagaagagt gatataaaaa tacatgaatt tttaactcaa ttttacatag ataaaatgtt
     6421 aaatttaaaa gaaggtaaaa agaatgttgg gttatgtaaa aagataatag ctgaaagtga
     6481 gtcttttagt gaaagtttaa gtctacttgg gatgaaaaaa gaaaagatat ttatagaagc
     6541 tttaaaaagt acaataaacg attattactc tgttgtcgat atagaagagt tgaaatccaa
     6601 agacaaaata gtaataacta ctccttattc atatatatca tcaaatatag atagaagtat
     6661 acaaatatgg gtagatatag gaagtaatgc ttggaatatg aaaatagaaa aagatataag
     6721 taatttaatt gtacttagaa aatcttttga agaaaaaaag atttatacaa atgaaatgga
     6781 agaatattat aaaaaatact atctatacaa tacagtatat aatttgttat taaatgcaaa
     6841 gaaaatctat gcatttaaaa gtgaatatgc gattaatggt tatatccaag agagtatatt
     6901 atatagtata cttttaaaaa tctcacataa aggagataaa aattatgatt aatataaatt
     6961 atagagaaga ccagatacca ataataaatt acgataatgg tactatggca gtaccagcag
     7021 taccaggtgc tggaaaaaca tttataataa caaatttagt agctaaacta ttacttgagc
     7081 agaaacataa gggtggtaaa atattaattc ttacatatat gaatagtgct gtaaataatt
     7141 ttaaaggtag aataagaaaa attcttgaag agaataaaat agatgatact aattcgtatg
     7201 aagtaatgac tatacacagt ttggctgtaa aaataataaa ggaaaagcca gaagttgtaa
     7261 tgttaagtga ggattttaac atagctgacg atttgcaaaa aagtattata ttaaatgaat
     7321 gtataagtag atttagattt gaaggtggag aaagagcttt tagatggttt ttaaaggagc
     7381 aaaaagatga acgctggaaa gaaataactc ttgaagcctg ggaaagaggt ttttttgaat
     7441 ttgcaggaaa tgcaataagt gaacttaagt ataagggaat ttctccagat aaccttgaag
     7501 aaatattgtc aagaggacac aaaggaatgc ttaaaattat tttaccaata tataaattgt
     7561 atgatagaaa attaaagcaa aatggattgc tagattatga tgatatatta attttagcag
     7621 aaaaaacttt aagcttagac gaagggctta gaaagaagtt tcaaactaga tacaaatata
     7681 tatttgaaga tgagtgtcag gactcaaatg aaattcaagg taatataata aaaattatat
     7741 ctagcgaaaa taaaaaccta gttagagttg gagatataaa tcagagtata acaggaacat
     7801 tttcatcatc agaccctaaa ttttttaaag aatttataaa tagtgcagat ttctgttata
     7861 ggatggatat gtcaaatcga agttcaaaag atatacttga tttagcaaac actcttgtca
     7921 agtatgttac aaaggaattt agacagaaag aatgtagaca tgctcttgaa gatatgcaaa
     7981 taaaaactgt gccaaatgga atgggatata aagaaaatcc taatccagaa cattataata
     8041 taaatgtaaa gtggtatgag agttggaaaa ttgaaataga gcaaactgct agatatgtta
     8101 aaggtataaa aaataaatat ccagataaaa gtataggtat attggttcca tttaatgaac
     8161 aagtgacaca agttgctaaa gaacttagag agaataatct aacttttgaa gaattaggac
     8221 caaattcttt gaataaaagg aaggttataa acaacatagc atatattatt gattttatgt
     8281 tacattgtga tgatgtagaa aagttaatag ttgtacttaa taaagttttt atagatactg
     8341 ataatgaaga agaaataaag tattttttag aagaattaaa aaactattct actgaagaaa
     8401 ttatttataa ttttgaattt agtaagttag ataatgattt aatagaattt aaagaagaaa
     8461 gtgacatata tttaagtttt aaaagaggaa tagatattat aaaacagata ttagaatatc
     8521 caataataag gttagacctc ttagttttgt ttattgggaa aaaacttaat ttagaaaaag
     8581 aagataaagc tgtactggat tatatatctt tttatataaa gtatctagta tcagaaaata
     8641 taaatatgga tttagaagat gtatataatt tattgtttga tgtaaagaac agggtgttta
     8701 atcatataat agatgtggta tatgagataa atggatatga accagagccc ggtagtataa
     8761 cactatgtaa ctatcataaa tcaaagggga tggaatggga ttgtgtgttt ttactaggtc
     8821 ttgttgaata taatttccct gacaatatta atcaaaagtt tcagagtgat aaatggtatc
     8881 taaaagaaaa atataaaaac cctatggcaa ttattaaatc agaagttgaa gctatattaa
     8941 aaggaagtat ttcaacagat tatgctcata aaacgaagat tgattctata aatgaaaaga
     9001 ttagactttt atatgtaggg attactagag caaaggaaat gttagtactt tcaggaagtg
     9061 cttatagaga tgaaagtgat atagggaata agagaaaaga gcaaaaacca tgtatatatt
     9121 taagcagact taatcaacat attattgaaa aaaggtcaaa ttaatatata ataagttatt
     9181 aaaagattgc ttgaaatata tctaaaaggg gataaaacaa tatgtacagt aagtaactac
     9241 taaaaaataa agttaagaga gaagtgatat gagtgaacga taaattaaaa tatttttctt
     9301 atagtcaaaa ttcaattaac acatataaat catgtccatt gaaatttaaa tataaatata
     9361 ttgatagaat aaattggaaa aatgatgatg ttggtagtag agagtattat gaaactttaa
     9421 aaacaggaag agattttcat ttgatttgtg aaagatattt tagcaatata cctcttggaa
     9481 tctattttaa tgaggatgat aaaaatagta aaaagttttt aaagtggatg gaaaatataa
     9541 aaaaagttgt tcctattgaa aaaggaaaaa cgtatttgcc agaatatgaa gtaaggatga
     9601 cattaaatgg ggatataata caggcaaagt atgatttagt tgtaattgga aataacaata
     9661 tagaaatatg ggattggaaa accgagtcta aaaagattga ctataaacac gtagaaaata
     9721 gaatacaaac tattgtttat atgtttttag caaaagaagt aatacctaaa gttttaaaaa
     9781 tggacataaa tgttaaagat ataaatatga gatattatca acctgaattt gatgacttac
     9841 caattaccat tttatatgat gaagaaaaac atgaagcaaa tagaaataaa attcaaaatt
     9901 atataagtat gataaaaaac actaattatg aagaacatac atacgaagac aatctataca
     9961 atgatataga aaaggtatat aataaagaag aacgtatgtg ctatagaaat aaaaagcatt
    10021 gcaagtattg tgaatttaat aagttatgta atggcaaaga aatagattat agtattttgg
    10081 aggcggaaat ttatgggact taggtttgta ttaggtagaa gtggaagtgg aaaatccacc
    10141 tatatactag atgaaattaa aaaagaagct caaaaaaatg aaacgacatc aattatactt
    10201 ttagttccag aacagtacac atttgaggct gaaaatagag taagtaaact atttttagga
    10261 aaagaaaaag ataagtatct aagagttagg gtacttagct ttaagacact aagtaatata
    10321 gttttttctc aagtaggagg attgacagat gtaaatataa attctagtgg aaaagcaatg
    10381 atggtatata gagctattga agatgtaagt gaagaattaa atgtgttttc aaaatcaaaa
    10441 tcgcagtctg gttttgtaag ttcaattaca gatatgatat cagaaatgaa acaatacaat
    10501 ataagccctg aaatgttaga aaatatatct ggagaattgg ataatgaaac tttaagttta
    10561 aaattaaaag atataagtaa aatatataat tcttttgaag ggaaattgca tgaaaattat
    10621 gtagatgctc aagatatgtt aacttcattg gcaagtaaaa ttgaattaag cagttattta
    10681 gatggagctt gtgtttatat agatgaattt acaggtttta caccaaatca gtacaatgtc
    10741 ataaagagta tattaaataa gtctaaaagt gttaatatct ctttgactgt agatgatatc
    10801 aactatattg ggtacagtaa atctgatatg ttttctagaa cgaaatttac ttattcaaaa
    10861 ttgactcaat tgtgcaacga agaaggcata aaaatattgc cacaagtaaa tttaaacaca
    10921 ggtgttataa aaagatttga aaaagttaag gaattacagc acttagaaag attttataat
    10981 gcttatccat ataaaatata ttcaaatcca actgaaaata taaagataaa agagtttaac
    11041 aatttatatt cagaggtgga agagatagcc agagaaattg tacatttagt tagagataag
    11101 aatgtcagat atagagatat aaccattgct acaagagatt taaatagata tgatttttta
    11161 gtacactcta tatttaatga atataatata ccaaacttta tagataagaa gagagaggca
    11221 aaaagtaatc caatagttat tttaattatt tcagctttag aaatgaagaa tagacgatat
    11281 gggtatgaaa ctatgtttag gtatttaaaa agtggactta taggaattga taatgatgat
    11341 ataaatttac tagaaaatta tgtgttggct aatgggatta aaggtaaaaa atggtttgat
    11401 gaaaaatggg attatagaat aacccaaagt ctttcaggtc aagaaagtga atttgaactg
    11461 gaattaaaag agaaaataaa tgaaataaaa aatagagtat tagagcctat agtaatttta
    11521 caagaaaaat taagaggaaa aaatagagta aaagaaatct gtagatatat atatgaattt
    11581 ttattagata taaatatgcc agaaactata gaaagtttaa tagtaaattt taaggataaa
    11641 ggggaacttg atgttgcaaa tcaatactca caagtttggg acatagtagt ggacatatta
    11701 gaccaaatgg tggaacttat gggtgatgaa ataatttctt tagaaaaatt tataaaatta
    11761 ataaccttag gttttgatga atatgaatta ggactggttc ctcctagtat agaccaggta
    11821 cttgtaagta gtgtggatag aatgaaaaat ccagacacga aatatttata cctagttgga
    11881 actactgatg gtgtgtttcc gctgattaca aaggatagtg gaatattgag tgataatgat
    11941 agggaaagtc ttggaaacaa aggaatcgaa gttgatattg atagtaagac taggagtttt
    12001 gaagaacagt ttcttgtata taaagctctt acttcgacat ctaagaattt aaccataaca
    12061 tatccaatat cagaccatga aggtaaaacg cttagaccat caataattat atcaagactt
    12121 aagaagatat ttccaaatat agaaaataaa agttatttag tggaagaaaa taaaagcact
    12181 gataaagata tacttaaaaa aataacggtt aaatccccta catttaatga acttataaac
    12241 gtaataaaaa attatgacag tgatggttac aatacagaag agattaatag tatttggtta
    12301 gatatatata ggtactattt aaaagatgaa atctatagtt ctataacaaa aaaagttata
    12361 aaaggtctta gttatactaa tcaagttcat aagattgaag agaaaaaaat aaggtcattg
    12421 tataaaagta attctcttag catatctagg cttgaaaaat atgctgaatg tccatttgct
    12481 tactttatac aatatggtct aaaagcaaaa aagcgtaaag agtattcatt tacaccacca
    12541 gatttaggaa cttttattca taatatttta gataggtttt caaaagaact tttacaagat
    12601 aatttaactt ggagagatat tgatgaaaaa tatatagaat taaagatagg tataatagtt
    12661 gatgaaataa ttttaaagat accaggatat atattaaata gttctgagag gtataaatac
    12721 cttgcatata gattaaagaa tatgttgaca actgctataa ctataattag tcagcaaata
    12781 aaacaaggtt catttgaacc aatagattat gaagttaaat ttggcaacaa tggaaaatat
    12841 ccacctataa aaatggtgtt agaaaatgga caagaagtaa gtttaattgg acaaatagat
    12901 agagttgatg aatttgaaga aggagaaaat aaatatataa gaataataga ttataagtca
    12961 ggcaataagt ccataagtct aacagaaata tattatggtc tacaattaca attacttgtg
    13021 tatttagatg caatccttga aagtgcaaag gatgaagata taaatataaa tccagctgca
    13081 atactatatt gtagaataaa taatccaata gcaaaattta atgaagataa agatgatgaa
    13141 gaaatacaag aagcaatatt aaaagaactt agaatgaagg ggcttgtagt aaaagactct
    13201 cacatagtta aagagatgga taaatcatta atagatggag aaaggaaaaa ctctttagta
    13261 ataccagtag gtcttactaa ggatggaaat gtaggtaaaa gtacttcagc aataagctat
    13321 gaggatttca aactgcttag aaaatatgta aggcatgcta taaaggattt atgtgaagaa
    13381 atgttaagtg gagaaatacg tatagctcca tataaacata aagatggaac atcatgtgac
    13441 ttttgtgatt actctgctat atgtcagttt gattcaacta taaaagataa caagtataaa
    13501 aatttaaata ataagagtaa tgaagagata ataaaaatga tgaaaggaga tgttaattag
    13561 tgagttctcc taaatggaca aaagagcaat tagaagttat agaaagtaga gaatgcaatt
    13621 tattggttgc tgctgctgct ggttcaggaa aaacagccgt tctagttgag cgtataattc
    13681 aaatgataac aagtagagaa aatccaattg atatagataa gttattagtt gttacattta
    13741 ctaatgctgc tgcgtcagaa atgagagaga ggatagggga tgctatagga aaagccttag
    13801 atgaaaaccc agaaaataaa catttacaaa accaacttgt tttattaaat aaatctagta
    13861 ttacaacaat acactcattc tgtttggatg ttataaaatc aaactttcat agaataaact
    13921 tagaccctaa tttcagaata ggagaccaaa ctgaatgtgc tattttgaaa caagaagcaa
    13981 tagaggaagt atttgaagat ttatatgaag aaagagatga ggggttttta aatttagttg
    14041 aaagttatgc agaaagagga ggagataaag aagtacaaga tattatcttg ggaatatatt
    14101 catttgcaat ggcttctcct gaacctaaaa aatggttgat tgactcagca gaacgattta
    14161 atattgatga aaattttgat ttttcacagt ctatttgggc tagagctata ttagacactg
    14221 taaaaataga gattaatggt ctttgtctaa atatggaaag ggcattaaaa gaagtggaat
    14281 caatagaaga attagaaact tttgcagaaa agttatccgt tgaatataaa aaaattgctg
    14341 acatttcaca agcatgtaat aaatcttggg atgaggcata taaaaagatg gcaagtatgt
    14401 cctttgaaaa ttatgtaaaa ggagtaaaaa gaatttcaaa agatgctcct tcgtatataa
    14461 aagaatcaaa agaaaaggca aaaactataa gagataagac taagaagtct ttagaaagta
    14521 tagtaagtgc tacattcaat aaagataatg attctattag agaagaaata aaatatcttt
    14581 ataacatagt taaaccaata tctagtgttg tactgagatt tgaagaggaa tatagcaata
    14641 agaaaagaga aaaaggaata atagatttta acgatataga gcattttgca ttaaatatac
    14701 ttacagatgt agatgaaaaa ggtaatattg taccttctga tatagcagtt ggatgtagaa
    14761 acaagtttta tgaaatattt atagatgaat atcaggatag taatctagtg caggaagtac
    14821 tgcttaaagc tgttgcaaat acagaaactc caaatagatt tatggttggt gatgtaaaac
    14881 agagtatata tagatttaga caagcaaagc cagaattatt tttgcaaaaa tataataact
    14941 ataatgataa aaagggaagc tcccatagaa aaataatgct ttacaaaaac tttagaagta
    15001 gagaagaagt tgtagatgct gtaaattata tatttgaaaa tataatgaat gagaatatag
    15061 gagaaattga atatacagaa aaagaaagac taaatttagg tgcaaatttt aatgtggata
    15121 cagacgagaa atctattatt ggtggagcaa cggagataca cctaatacaa aaagataata
    15181 aacttgatga tgatattata aatgataaag acgatagaat taataacaaa gaaaatgaaa
    15241 tagaggaaga agaaaaatta gataatatac aacttgaggc tagaatggtt ggaaacatta
    15301 tcaaagattt aatgaaagtc aatgaagatg gaaaaattca aaaggtctat gataagggaa
    15361 tagatggata tagacctgtg gaatttagag atatagttat tcttttaaga gctacatctg
    15421 catgggctcc agtgtttgca gatgaattga tgaatatgga tataccaacg tatgctgatg
    15481 ttggagtggg gtattttgat acgattgaaa taaaaacaat attgtcatta cttcaaataa
    15541 tagataatcc aatgcaagat atacctctta tatctgtatt aaaatcacct atatttggat
    15601 ttacaccaga ggatttgatt gatattagag ttcagagtaa agataaaatt ttttatgagg
    15661 ttttaaaaag tacagcagag tatgatggat ttacagattc tcaaaatgaa aatgagtctg
    15721 agtttatacc aagtgaagag tgcataaaca aaagtaaaga ttttttaatt aagctaaagg
    15781 aatttaaaga aaagtcaatg tacatgagta cagatgaatt tatatggtac ttgtacacaa
    15841 ggactggata ctatgcctat gttggagcat tgccaggtgg aagtcaaaga caagctaatt
    15901 taaaagtatt gtttgagaga gcaaaacaat ttgaagaaac cagtcttaaa ggaatattca
    15961 actttgtaaa ttttatagag aaattaaaaa aatctagttc tgatatggga agtgcaaaaa
    16021 cacttggtga gaatgcaaat gttgttagaa taatgagtat tcataaaagt aaaggtctag
    16081 aatttccagt agtcatatgc tctgcaatgg gtaaaaattt caatactcaa gattttaaaa
    16141 agagtatttt atatcatcat aatttagggt atggacctca atttgtagac tatgagagaa
    16201 ggatttcttt tccaagtata gccaaagaag cattaaaaag caaaataaat atagaaaatt
    16261 tatcagaaga gatgagagtt ttatatgttg catttacaag agctaaagaa aaattgatta
    16321 taactggttc tactagaaat atacaagata gtataaagag atggtcaaat ggaattgaaa
    16381 gtttagatac aatatcacaa tacgagatat taaaaggtaa aaatttttta gattggataa
    16441 tgccttgtgt attaagacat agggatttat ctaatctact agaagaagta ggattagacg
    16501 cagtttttaa tgttgaacat aattcaaaat ggtatggaaa gttgtggaat aagaatgata
    16561 ttttagtgga aaagaagagt gatgaagaaa aagaaagtat tgaagaaatt cttgaaaaaa
    16621 tagatgtaaa taatcctgac agtgattatt acggtgaaat agaagaaaaa ttaaactata
    16681 tatatccata tgaatttagt acaagaaaac ctgctactat atctgttaca gaaataaaaa
    16741 agatacaaaa taattatgaa gaagagttaa taaatactat atttgaacaa aaagtaatat
    16801 taaaaaagcc tctatttatt caaaatgaag aagaaagaga aaaaataagt ggaacagaaa
    16861 gaggtacaat agtccattta gttatggagg ttttagactt aaaaaatgta agtagtgtaa
    16921 atgatataaa atcacaaata agaggttttg tttcaaaagg tataataaca gaaaaacaag
    16981 ctagtatagt aaatccatat aaaatatata aattttttgc ctctaacata ggaaaaagaa
    17041 tgttgaatgc tgaaattata aatagagaaa aatcaattta tgcacaagta aatatgaaag
    17101 acatatatat ttatgaaaag cttataaaca atgacgataa aaaactttat gataatgaaa
    17161 gtgtcatgtt aagaggtata gttgatgctt attttgaaga agataatcaa atagttttgg
    17221 tggattataa aacagatttt gtaaatgagg aaaatataaa tcaaataata gagaaatata
    17281 aaaaacagtt ggatttatat gcagatatta ttgaaacttt aactggtaag tcagtaaaag
    17341 agaaatgtat atatttattt ggagttgatg aagctgtttg ttattagttt ttatatataa
    17401 attaaaccta aaagatagag gaaggggaaa aaatgagatt tattcataca tcagattggc
    17461 atctagggaa gtccctagaa ggccattcta gaattgagga acaagcaaag ttttgtgagg
    17521 aatttataaa aatagtagaa tcaaatgaaa tagatatggt tataattgct ggagatgtat
    17581 atgatacttc taatccccca gcacaagcag aaaaactatt ttatcagact gtatcaagac
    17641 ttgccaataa tggtcagagg tgtgtattaa taatttcagg aaatcatgat aatccagaga
    17701 gactctctgc aataacacct ctagcacatg aacaggggat acttatatat ggttatccat
    17761 taagtgctac gatagaagct aagtataaag ggtttgaaat aacatatgca actcagggat
    17821 gtacaaaact gaatataaat ggagaaaata ttgtggttgc tacattacct tatccaagtg
    17881 aaaaaagatt aaatgaagtt ttttcaagtg aagatgagtt tgagaaacaa aaaaattatt
    17941 ctgaaaaggt aggagatata tttagaagtt tagaagaaaa ttttagaagt gatactataa
    18001 atatagccgt atcacatata tttgtaatag gtggagagag tactgaatca gaaagaccta
    18061 tacagttagg tggaagtttt ttagttgaaa gaaaagattt gccagaaaag gctcaatata
    18121 ctgcactagg tcatttacat aaacaacaaa aagcttccga acgtttaaat gcatactatt
    18181 ctggttctcc acttcaatat agcaaggatg agagagcgta taccaaaggg gcatacatag
    18241 tggatataaa agctggtgaa aaaccaataa ttgaagatgt atattttaat aattacaaac
    18301 ctatagaagt atttaaatgt aatggaatag aagaagcact agatatatgt gagcaaaatc
    18361 aagatagaga gatttggtca tattttgaaa taaatactga tgagataata tcacagaatg
    18421 aaattaaaaa aatgaaggaa ctattaaaag acataataga aataaaacca ataataacat
    18481 cttgttatga acaagaaagt gtagatataa aagaaaagtc tatggcagag ttgtttagag
    18541 agttctattc ttttagtaaa ggtgtagagc caaaaggaga acttatggat ttatttctag
    18601 atataataag tgaagaaggt gaatcagcag atgagaccaa ttagattaga acttacaggg
    18661 cttaatagct atattgataa acaagttata gactttgaaa aattaattga aagaggtttg
    18721 tttgggatat ttggaactac tggaagtgga aaatctacaa tcttagatgc aataactatt
    18781 gctatgtatg gaaatatatc cagaaataca aaagaatata taaacagtgt gtgcgataag
    18841 gcaattatat catacgagtt tgagatagga agcaaaaaca ctagaagaag atacattgta
    18901 gatagaacca ttgctagaag caaaacagga acaaagacct ctaatgctag attaatagaa
    18961 gtattgaatg acaataccca aaatgtttta gctgataaag ttgtggaagt aaatgaaaaa
    19021 gtagcacagg ttgtaggatt aacagcaaat gattttacta ggtcagtagt tttaccacaa
    19081 gacaaattta atgagtttct aagattatca cgtgcagata gaagagacat gttagagaga
    19141 atttttaatc ttgagaaata tggaagaagt cttggagaaa aagtgaaaaa gagaaaaaat
    19201 atacagttac aaaatttaaa agatttgaaa tcaaaattaa gtcaatatga tggaattaca
    19261 gaggaagtat acaacaatat aaaccaagaa ttaatagagt taaaaaattt ggaaaaagat
    19321 aaggataatg ctttagacct agctcaaaaa tcttatgaag aaagtaaaac agtatatgaa
    19381 gaacaattaa agttagaaaa aaatgaactt agaaaaaatg agcttgattt aaaaaatagt
    19441 gagataaaag aaaagattgc tcttgtagaa aatgatagta acgctaaaaa ggtagaccca
    19501 tacataagtt cagttcaaaa tctagaaaaa aagatagatg aagatagctt cgcagtaagt
    19561 aatttagaaa agaaattagc aatattaaat caggagcttg aagttacaaa aaatagatat
    19621 gaaaaaataa acaaaattaa gaatgaagaa gtccctaaat tgagtgaaga aaaaataaga
    19681 ttgcaacaag caataaaatt agaagaagag ttagttttat tagatagaga gttaaaagag
    19741 ctaaaagaaa gtggaattaa tttaaataag actaaagttg aattagaaaa agtaaagcag
    19801 gtgtcagagt ccagaaaaga tgcagttact aaatctataa aagaagttga aggaaagatt
    19861 gataaagtaa atataagtgc agaattaaaa caaaaaatat ttctagcata tgaatacgag
    19921 aaagattaca acaacgtatt ggaagaaaaa aatcaaaagc taaataaact agaagaaata
    19981 ctcaaagaga cagaaaatat aaatttaaaa gttagatata ttgataaaga taagaatgat
    20041 gtaaatcgta atttagaaaa tttatcactt cacttggatg ttcttcttaa aaaatgtcct
    20101 ggtaagagtg ctgatttact tttaaaatct gaatatgtta ctgaattaag aaataaagca
    20161 aataatacta aagaaaatga aattaagaaa agcagtatac aagatgaatt aaagataata
    20221 cttgaaagta agtttaatac tgaaagggaa gtaaatttac taaatgaaaa acttgaaaat
    20281 aatagaaaga acagagatga cttagaaaaa gaattagaag aactaaagta tttaaattta
    20341 gcctctgaac ttagacgtga attaaaagaa aatatgcctt gtccagtttg tgggtcaaaa
    20401 catcatgaaa atcatgatat aactaagtat gatgaaaata tatcttttgt caaagaaaaa
    20461 ctggagaaat tagaaaaaga aaaaataagt ataagaaaca atatagagga attaaatgct
    20521 aaagtaagtg ggtatttatc tatagaaaaa atgaagacta aagaacttga agatgtaaaa
    20581 ggaaaactag gggggattcc atctagtcaa ttattaaaaa aacttgatga agaacaaaga
    20641 aaacttgcat tgttaaaatc taatattcag gagtgggaga aagaaaaaga gagtactgaa
    20701 aataaagtaa cattagctaa agaagaaaaa aataaaattg aaaaagaaga attaaaaata
    20761 agagagagtc tgaataatta taagaaatta acaagagatt taaacataga aatagaaggt
    20821 ttagaaaata aatgtaaaaa acttaaacag gaatacctag gattaaagac gattactaaa
    20881 gtctctaatc tattgtcaaa agtaaaagaa ataagagaga atgaaaaaga attagaatta
    20941 ctaagtactg ggcattcaaa tttattaaaa aatagagact cactagatat taaaataaga
    21001 gaaaatgaga gtaaactaca tgaaatagag atagaattaa taaaggctag agaattatat
    21061 gtagaaaaga aattgagtag agataataag tataaagaag ttattagcat aacgaaaggt
    21121 gatttagcta aaaatctttt acataatgtt gaagaaaata tatgtaagat attagaacaa
    21181 gaagaatcaa gtaagaaaaa attggaagag caaagattag aatatgaaaa aaacattgct
    21241 gaaaagcaca atatagatgg tagacttaaa actgctaagg aacaatataa agaccaaaaa
    21301 tcaacactaa ataaactatt agcagaaaat aaatttgaaa gtatatatgc agtaaaaaga
    21361 gctttattag atactgatgc agtaaaaaaa ttgattgaag aaatactaga gcatgaagaa
    21421 gaacaaaagt tattatcatt taaaataaaa tcatcaaaag aaaaattaaa tggaagaagc
    21481 ataaagaagg agtactttga acagttaaaa gatgaaattt ataatttgaa agtagaaata
    21541 ggaaatatat ccaaagatat tggagctaat caaaatcagt taataacatt aaaagactct
    21601 ttagataaga taaatgattt taataaacaa cttaaggtag ttgaacacaa tgtggactta
    21661 ttggaagaat tagataaatg tgtacaagga aataaatttg tagaatatgt ttctacaaac
    21721 caattaaaat atatagcgtt ggaagcatca aaacgattgg atggaataac aaaaggaaga
    21781 tatgcacttg aaattgactc aacactaaac tttgtaatga gagataactt taatggagga
    21841 gaaagaagaa gcattgatac tctatctggt ggagagacat tcttgacatc tctatcatta
    21901 gcacttgctc tttcatctca aatacaatta aagggaagtg caccattaga atttttcttt
    21961 ttagatgaag gttttggttc attagatagt gaacttttag aaattgttat agaatcattg
    22021 gaaagattgc atagtaataa tctaagtgtg ggtataataa gtcatgtcga ggaacttaaa
    22081 aatagagttc ctgttaagct attagtttca tctagtgagg ctggaatagg ttcaaaagta
    22141 aaaattgaat atagttagaa aatatatgtt aattgaatat attatctaat atttccaaat
    22201 aatatgtaat tgtgatataa taaaaactaa tattaaagtt atttataatt aatattaata
    22261 taaaaacatg taagtaggag tgtggtaagt gttggaatcg cccaataatt tgattcagat
    22321 tattttttta atagtattac ttatagggtc tgcatttttc tcagcatctg aaacggcttt
    22381 aatgtcgttg agtaaaatca gaatcagata tatgcaagat gaaggagtta aaggagctaa
    22441 gttagtaagt tcattaatag aaaatccaaa taagctttta agttctatat tggttggaaa
    22501 taacgtcgta aatatagcag caacatccat atcaacatct ttgtttatag gattaatggg
    22561 agaaaaagga gtggcacttg ctacagctgt tatgacagta ttagtattaa tatttggtga
    22621 gattactcca aaaacaatag cagcaaataa ttcagaaaag gtttctctat tagtatctaa
    22681 acctataaaa gcaattatat ttatattaag accaatagta tggatattta atataattac
    22741 aaatattata tttaaattat ttggtataac aaataaagga gctaaatcat tcataactga
    22801 agaagagcta aaaactatgg taaatgtaag tcatgaagaa ggcgtccttg aaatggaaga
    22861 gagagaaatc ataaataatg tttttgaatt tggagatatg caagctaaaa atgcaatggt
    22921 acaaagaata gatatggtag ctatagatat ggaagatagt tatgatgaga taatacaggt
    22981 atttaaaact gaaaaattaa gcagaatgcc tgtgtatgaa gaaactatag atgatattgt
    23041 aggaatactt aatataaaag atattatatt tttatctgat gaagaaatag agtcatttga
    23101 tattaaaaat tatatgagag aacctttttt cacgtatgag ttcaaaaaga taacacaact
    23161 tcttgaagag atgaaacttg aaaaaagtca gatggctata gttgtagatg agtatggtgg
    23221 aacatctgga cttttaacta tagaagattt agtagaagtc attgttggag atatagagga
    23281 tgaatatgat gaagaagagg atgaaataca agttataaaa gaagatgagt atattgtaga
    23341 tggaagtact aaaattggtg atgttaatga acttattggt gtaaacttgg aatctgagga
    23401 gtttgattcc ataggaggtt ttataattgg acacctaagc agattgccag aagaaaatga
    23461 agtaattgag gttgataata taagattttg tatagaaagt atagaaaaaa atagaattaa
    23521 aaaaataaga atatatacat aatgtaataa tgttcttaaa tttattagaa gtgtataaat
    23581 ataaaagata tcttttatat ttatacactt ctttttattt tgtattcaaa taaaactagt
    23641 atgaaatgga caaaataaaa atataaatta gaagaaagga gatttttaag atgagcatac
    23701 tagaaaataa ttttttatac aaattaaaaa acaacactat ctttttttgt atatatacat
    23761 tgttatttat ttttatttac aaagcgtttc catacatagc tccatttttt ctaggtgcta
    23821 taatagcact tatgattaat cctatatcac aaaagttaga aaataagttt cgtataaata
    23881 aaggtatctc tactcttgtt ctaagttttc ttgcagtggc tatagtcagt acagttacta
    23941 caataatagt aataaattca atgaaagaat taatgggatt tttaaataat atatctgcta
    24001 atcctgagga tataagtaat actattatgt attttttaaa taaaataaat gactttatga
    24061 aatcatttca agaaatagcc aattttgatg tagaacaatt agtaaacaaa ttcagtggtg
    24121 aagtaatgca aattacaaaa aatcttctta ctagtatact tggattggct acttctattc
    24181 catatattat aatatttata attaccttgt ttattgctac gtactttata gcaaaagacc
    24241 ttgataagat tgaaaatagt ttttataata tgtttactgt tgatgttaga aagaaggtaa
    24301 aaaacgtaaa gaaagaggca gggctttcac ttgttggata tataagggcc tatacaatac
    24361 ttatggctat aacatttttt gcaatctggg gaagttttgc tttatttgga ttgaaatacg
    24421 gtctaatagt agggtttgta ggagctttaa tggatttaat tccattccta ggtataataa
    24481 caatatatct tccagtaatt gtttattatt tccttataaa aaattatttt atagctataa
    24541 gtatgactgt gatatttttt gttttgtctt tgattagaga aatacttgaa cctaaactag
    24601 tatcagtaaa tgttggacta aatccattag ctaccctagc agcgattttt attggaatcc
    24661 aagtaaaagg aataattgga gtaatttttt gtttgggttt ggtatgtatg catgatatac
    24721 ttaagaaggt ggatattttt taattataat aattgataga ttttttaaaa ataaaatttt
    24781 attcacaggt aaataaaaaa atattaatct tattaagaaa gagggtatat acgtgaaaca
    24841 aaaagtagtg gaaagatttt taaaatatgt atcatttgat acaacatcta attctcagtg
    24901 tgagaactgt ccatcttcag aaggtcaaag agttttagct aagtatatag ttgaagagct
    24961 aaaaacaatg ggtgttgatg atgtgagttt agatgaaaat agttatataa tggctacttt
    25021 aaaaggaaat actgatggtg tggatactat aggattcata tcacatttag atacaattga
    25081 agatgttagt ggaaaagata ttaaaccaag aataatagaa aattatgatg gaaaagacat
    25141 agttttaaat gaagcgttaa atgttattac gtatgtaaag gattctccag agttggaaga
    25201 atttaaaggt gacgatttaa tagtcactga tggaacaact ctacttgggt ctgatgataa
    25261 agctggtata gctgagatag tgactgcaat agagtatcta ataaatcatc cagaaattaa
    25321 gcatggagat ataaaaatag gatttacacc agatgaagaa attggtagag gtgctgattt
    25381 atttgatgtt gaaaaatttg gagcaaaata tgcatatact ttagatggag gaatagttgg
    25441 agaacttgaa tgtgaaaatt ttaatgctgc aaatgcaacc attactatac atggtagaaa
    25501 tgttcatcca ggttcagcta aaaataaaat ggtaaatgct atacatatag ctgctgaaat
    25561 atcagaaatg ttcccagcag atgaaagacc agaaacaaca gaaggatatg aaggattttg
    25621 gcatttaaat tctataggtg gaaatgtcga gaatgtaagt atggcttata ttattagaga
    25681 ccattgtaaa gaaaaatttg aaaatagaaa atctattatg atagagaata tagaaaaaat
    25741 aaataagaaa tacgataata gagtggaatt ggatttaaaa gactcttatt acaatatgaa
    25801 agaaaagatt gagccagtta tgtttatagt tgatatagct aaagaagcta tggaggaatt
    25861 aggtataaaa ccacgtttag ttcctgtaag aggtggtact gatggagcaa gattatcttt
    25921 taatggatta ccttgtccaa atatatttac tggtggatta aacttccatg ggaaaaatga
    25981 gtgtatacca gttagttcta tggagaaagc tactaaacta attgtaagaa tagctgaaaa
    26041 atatgctgaa agagtttagt taaaaagtat ttaaaaatta agcatttaaa gttataatag
    26101 ctatatcatt ctttataagt caagctgatt ataagcttgt gttgtactta aaatggtata
    26161 gctatttttg taattatttt tataaaagat aattattttt agtttagggt atcaataaaa
    26221 agtatgaata tcaataggta ttaacattta aaagggggaa ttgtattgaa aattaaatct
    26281 aaaaaaataa tagcatttgc actttcactt gggattatgt tgccaagttg tgtatatgca
    26341 gacactatta gtaaaaatca aaatgcacag gtagatatga acaattatct tcaaggtaga
    26401 aaatctccaa aggaggaaaa acttattggt gaagatagat ttgatacagc gataaaaata
    26461 tctcaaagtg gttggaataa tggttcagaa agggtgtttc tagttaactc aaattcattg
    26521 ccagatgcat tggcttcaac tcctttggca agtaaattgg atgcaccaat actgcttaca
    26581 aataaaaata gtataccata taatgtaaca gaggaaataa aaaggcttaa tccagcagaa
    26641 gttattttaa ttggaagtga aggagctatt tcatcaagtg taaaagaatc tcttgaagat
    26701 atggaagtat tagtcagtag aatcggtgga gcagatagag aagaaacatc tttacttctt
    26761 actagacagc tagatgatac tggtgattta ggtgtctcaa aagtggcagt ggttaatggg
    26821 tataatggac tggcagatgc aacttcaata tcaagcccag ctgcaagtga taatacagct
    26881 ataatatata caggtaagga tagcataaga agtgaggcaa agaattttat tacacaaaac
    26941 tcaactaaga catacatagt aggtggggag tacagtataa gtaaaaaact tgaaggacag
    27001 ttagtaaact cagaaagact agcagggaca gatagaaaag atactaatgc gaaagtttta
    27061 gaaaagtttt atggaaaatc aagtaaggtt aataatatgt attttgcaaa agatggaagt
    27121 ggtagagaag ctgatttagt tgatgggctt gctgctggag tattagcatc aaaaacaaag
    27181 tcacctgtag tacttgcaag tggaagttta agtagtgcac agaaaagttt tataaaaaaa
    27241 gtaaaggctg ataaatttgt acaagttggt ggaggtaaaa atagtaaacc atatgcagaa
    27301 gcattagcct tacagtaata ttgtgtaaat caaaattaat atataataat agcaagtaaa
    27361 ccaagttgca cttcaatata tataataaaa taagtaaact aagctatgtt tcaatataat
    27421 at
//
LOCUS       DAMJYX010000044        27242 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJYX010000044 DAMJYX010000000
VERSION     DAMJYX010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.044"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(60..735)
                     /locus_tag="PC479_001979"
                     /pseudo
     CDS             complement(60..735)
                     /locus_tag="PC479_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1283..2812)
                     /locus_tag="PC479_001980"
     CDS             complement(1283..2812)
                     /locus_tag="PC479_001980"
                     /inference="COORDINATES: protein motif:HMM:NF012862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HDF2804526.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
     gene            complement(3511..4941)
                     /gene="dcm"
                     /locus_tag="PC479_001981"
     CDS             complement(3511..4941)
                     /gene="dcm"
                     /locus_tag="PC479_001981"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDF2804527.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            5154..5870
                     /locus_tag="PC479_001982"
     CDS             5154..5870
                     /locus_tag="PC479_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDF2804528.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEDIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTVLLK
                     KTISNLADEISKDKSLFNQK"
     gene            complement(6115..6393)
                     /locus_tag="PC479_001983"
     CDS             complement(6115..6393)
                     /locus_tag="PC479_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804529.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(6641..7975)
                     /locus_tag="PC479_001984"
     CDS             complement(6641..7975)
                     /locus_tag="PC479_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2804530.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(7988..8731)
                     /locus_tag="PC479_001985"
     CDS             complement(7988..8731)
                     /locus_tag="PC479_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804531.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(8752..9066)
                     /locus_tag="PC479_001986"
     CDS             complement(8752..9066)
                     /locus_tag="PC479_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804532.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(9070..9264)
                     /locus_tag="PC479_001987"
     CDS             complement(9070..9264)
                     /locus_tag="PC479_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804533.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            9548..10264
                     /locus_tag="PC479_001988"
     CDS             9548..10264
                     /locus_tag="PC479_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2804534.1"
                     /translation="MIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFD
                     NYSAKLIGNNISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALAT
                     YTKIEDLFGFPLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDK
                     SEFDLVIHILKNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGL
                     VENFIQENSNGLIEDEDEEY"
     gene            10361..11113
                     /locus_tag="PC479_001989"
     CDS             10361..11113
                     /locus_tag="PC479_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2804535.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            11103..12269
                     /locus_tag="PC479_001990"
     CDS             11103..12269
                     /locus_tag="PC479_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2804536.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(12386..12616)
                     /locus_tag="PC479_001991"
     CDS             complement(12386..12616)
                     /locus_tag="PC479_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804537.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(12865..17436)
                     /locus_tag="PC479_001992"
     CDS             complement(12865..17436)
                     /locus_tag="PC479_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2804538.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
     gene            complement(17841..18755)
                     /locus_tag="PC479_001993"
     CDS             complement(17841..18755)
                     /locus_tag="PC479_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HDF2804539.1"
                     /translation="MELVKNIHKNKSKDEIFKELLENLKGTIASWDYYVNWNKALENT
                     RKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVIPIVIASRDNEFKILDPSDEDM
                     FKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQKNIKNLVDYVFGVEVGLDTNA
                     RKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKISEKWSIEVPTDKSKRKYDFAI
                     NNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKDGGFTFCWITDGLGWLESSIPL
                     RDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(18774..19688)
                     /locus_tag="PC479_001994"
                     /pseudo
     CDS             complement(18774..19688)
                     /locus_tag="PC479_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
     gene            complement(20116..20465)
                     /gene="ssrA"
                     /locus_tag="PC479_001995"
     tmRNA           complement(20116..20465)
                     /gene="ssrA"
                     /locus_tag="PC479_001995"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(20623..21078)
                     /gene="smpB"
                     /locus_tag="PC479_001996"
     CDS             complement(20623..21078)
                     /gene="smpB"
                     /locus_tag="PC479_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2804540.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            21691..22518
                     /locus_tag="PC479_001997"
     CDS             21691..22518
                     /locus_tag="PC479_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2804541.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            23543..24007
                     /locus_tag="PC479_001998"
     CDS             23543..24007
                     /locus_tag="PC479_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2804542.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(24274..24918)
                     /locus_tag="PC479_001999"
     CDS             complement(24274..24918)
                     /locus_tag="PC479_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804543.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(24848..26137)
                     /locus_tag="PC479_002000"
     CDS             complement(24848..26137)
                     /locus_tag="PC479_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804544.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(26134..27006)
                     /locus_tag="PC479_002001"
     CDS             complement(26134..27006)
                     /locus_tag="PC479_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804545.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         9258 a         4274 c         2847 g        10863 t
ORIGIN      
        1 ttttagtatc attattggtg taaacaatga taatattaat aatattttat ttatatggtt
       61 taacatattc tatcaccaat aaattttaga aacccctctt ttatatcttt atatctatat
      121 ctactaatag gtacttcttc ttgactgtct aatattgcag tatttgactt tatattttct
      181 acatagttta agttagttat ataactttta tgacatctta caagttcctc tatatttaaa
      241 tctttttcta ttttttctag gatacatata gcatcaagag ttttattttt tgtatgtatt
      301 agcatgttct ttttttgaac ttctatatat ttttttgcta gattagattt tgtatgtgtt
      361 agatttattt ttattttttt caattttttt ggtacatgtt aatatgtgtt tttctagttc
      421 ttgaaaattt attggcttta ataagtatca ataagctctt acttcatatc tttcctgtac
      481 ataatttatt agagatgtta taaatattat tatctacttt tcttatgttt cttgcgatat
      541 ctatactatt tagttctgcc atttttatat ctaagagtag tgtatcctct caattaactt
      601 tgtaatgatt cttcttttga gctatatttt tttaattcga tattctttgt ttttattttt
      661 taatatatta tcaacttgat tttttattac gatttgtatt tctttttcat catcacaagc
      721 tgttattgta atgataacct ctcctaatat tgtattttta tttagattct tcactttagg
      781 ttaaaacttt ttaaatgtat ttatcaataa tatttaaatt aaaaatgtat aaaaatacct
      841 atatccaatt ggatataggt attcgtggat ttcaatggtg gagacgaggg gagtctataa
      901 gtggttattt gattgttttg ttgttttttg caagtgtagg tatatagcta tttagttatg
      961 atgttaaact gattttttcg gatatttttt attgtttatt tttgtagttg gggtattatt
     1021 gggggggggt ggatgatata atcttttaat tttataaaag ttccattaac agcatattat
     1081 acgtttttaa atatttacta gactttttca aaataaatat ctaattattt aaatcaatac
     1141 caatataatt tataaatgga tttataattg tatcttctat catgtttagt gtaaacaact
     1201 tattttaatt taaattcgta accaacaaag taccaatctg acttataatt ctatattagt
     1261 acaactatgg aaaaatttaa ttctatatct cctcacttaa tcttatgact tctcttaaat
     1321 ctttgtaata atcctctata ttcatttcca tacattgctt tctattttcc tcagttctac
     1381 tatgatccca ataaactcca aaattttgta tagttgacac taagtttata catttatcaa
     1441 accaatcttt aatttcaatt ggtggaaaat ctaaatttat aactttattg taacactcat
     1501 ccattttttt ctttctacta tctataattt gaaaaatatt atcttcacat attttcttat
     1561 tttcatattc attatagaag gatataattt cttcagatat gtttatcata tttgttaaaa
     1621 tttctttaac ttcgtatact gccttttctt tagtgattgt attttgactt atatatgatt
     1681 ttaagctttc gtagttatca tatgtttcag tgtgaacacc gttgataaaa aatttatttt
     1741 tcccatttac actataccat atatctttct cttgatattt atcaatccaa gtagttttac
     1801 acatatatat tccatttatc atatcaattt catctttgcc aaaatatacc catattatat
     1861 tagcatcttt tccttttaat ctatcttctc ctatttcact attataaact tcaacagact
     1921 taactatgtc aaaaacctcc ttagatattt ctgatatatc acaataggag aatttatttt
     1981 ttaatataac ttctactgaa tatcttttta ttatacttat atgtaaagat ttgacattgt
     2041 atattatgta atcgtcatgc tcttgaaaaa ctttcttttt tatataagat ttataatctt
     2101 ttacattaaa atatatatct cccagtatgt tagacctaaa gataggattt tgtttacagt
     2161 ttatataatt tttgttcaat atactcatac gcaaattaat atacttgctt attgctctta
     2221 aagataactc cccttttctt actatgtatt catttcttaa cgcttctgat aataaacttg
     2281 taaatctact aattgaacta tctggaagag aaaatgattc ttgatattcg ctacttgata
     2341 ctaacactgc acatcctttt ctatcaaaca aggaaaatat gtctttaata tttatttttt
     2401 gtttgtcact tacattaaat ttacctgcaa agcaacaatc aataaataca attttatttt
     2461 tacaatttaa tccttctata aaatcaatta caaattgaat agaaactaat tcatcactta
     2521 atactaagta atgcactccg ttattacata tgcatccatg ccctgaaaag taaaatataa
     2581 atgtatcatc atctcctata gaatcgctta tttcttgaaa agttttaaaa aataaatctt
     2641 ttctgacttc atactccata cctaaagttc ttatattatt cgaagtaatc cccagtcttt
     2701 tatgtaacac attattaatt tcaattatat catttttaca aaatagtaaa tctttatttt
     2761 taactatact atatttactt actcctacaa ttaatgcttt taagcttttc atattccatt
     2821 ccccctttat aatttttcaa tttaatatac ttaaaatgat gttttaattg ttttattgat
     2881 tttataaaag ctagtatcac actacttttt tgactttttc attatttact tttgtagttg
     2941 gattataatt tttcaaggta agtatatata tatttattta catcgcaccc atataattta
     3001 tatctctgat ttatctgacc tgaacttagt gtaatttaaa ccaatgtaaa atcatctatc
     3061 tcttcaccat atatttttta aaattcccca aaatattgat gttaatagaa tttcatgtgt
     3121 tttttgtatt ctactatcca taagaatttt aaagtgagta aatatttcca atcatttata
     3181 tcttatttaa tatcatttta ttccctcaag tatttattta aattgttatg ctttatttat
     3241 aaaattatac atacatttct aaagttagac atacatcttt aaaaaccttt aatttgtgct
     3301 actttttttc taaaaataat aaatatataa aaattatata tacaaaaaat taagttatga
     3361 ctagtttata aataagtaca taataaaatt aaagagagca attttttttg ctctctttca
     3421 aaatttatat taaattgtaa taataaaaat aaaaacgcta gttatctaag gctaatattt
     3481 tcaacgatta tataaatatc tacataacag ttaaattaca tactcttctt cagttattgc
     3541 aacttcctca gtgacatata ttttttctat tttattaaga taatctacta tacttaatcc
     3601 aatagccttt cctagcttaa caggaactgc atttcctatc tgcttgtaca cctgactaac
     3661 tgaccctgta aactcccact catcaggaaa actttgaatt ctagcatact ctctagttgt
     3721 gaatggtcta gtctcatctg gatgacacct ttcagtttgc ttttgtgcag gactacatgt
     3781 taaagttagg catggttcat cccaacttat tcgccttgcc atacctcttt ttccgccacc
     3841 gctataataa ctttttccca tataatctct tgccacctca tctggcaaat ctatccaaca
     3901 cccacctggt ggtaccatat ctaacacttt tttcttcctc tcaggatatc tagctccttc
     3961 cgattttgga acatttttaa gtgcatctct taacgtagca acatattcaa acgacttagg
     4021 gaacttaaaa tccacatgtg acaaatcttt tcttgttcct attataataa ctctttccct
     4081 cttttgtgca actccatagt tcacagcctt tagtatctca tattttacat tgtatccaat
     4141 ctcgctaaat acatctatca tagtctttaa tgtttttcca ccatcatgag atacaagacc
     4201 tctaacattc tctgctaaaa acaccttagg ctttaactct tctagaattt tagcatatga
     4261 ataaaacaat gtccctctta catcctctaa ccctagcttc ttacccgcat agctaaatgc
     4321 ttggcatgga taaccacctg atagcaaatc tatctctata ttattatcaa tataattctt
     4381 tattccattt tccgctattt taacaatgtc accctgtata acattccagt ctggtctatt
     4441 taattttaat gtatcacaag catatttatc ccattctact aatccaacct ctttaaaacc
     4501 agctttttca aggcctaatg ctaatcctcc tgctcctgca aataattcaa cacatgtata
     4561 tgctctttta gctagtacat tacttatatc tatatgttta ttatttttat ttctctctct
     4621 aggatttaat tcatattcac tgagttcttt atatgtacag tctctatgct ctatataatc
     4681 tttattactg ctacaaatat ttgtaaattt cagttgcttt tcatttacat cttccaaaca
     4741 cataatttca tcgaatccaa tatctaacac ttcacataat ttttctacat tactttttat
     4801 aggtgtaaat ttttctgata atattgcaga taactgattc ttagttatgc ccatatttaa
     4861 tgccagttga ttctgacttc ttatattttt ctctttcata atttttctca ctctattttt
     4921 atcaatagtt atttttttca tttagttcac ctcttaataa ataatatacc cttaaatagt
     4981 taataaaata cattaactat ttaaggatat attatcataa ttctaatttt tatgtaagta
     5041 gttataaaaa cttaaaataa aatatctgta aacaacctaa tattacctca taattttaca
     5101 cattatggaa tatattgtat aattacatta atacttttta gggggattat tttatgactg
     5161 aattagaaaa acaagaaata ctagataaag ccaaagattt ttttacaaat attttggttg
     5221 ctaatcataa aaagaatact attaagttaa atttaaaggc atttaatata aatccttttt
     5281 tagttaaata cttagctagt ttcttaacag gatcaaataa acctgaagat attgctaaag
     5341 cattgattta tccaagagtt ctaggcactt cgattaatac gtccttcgga actaatttac
     5401 aaaaattctg tattaccact ttagatggtt ttgcctctac aacttctggc attgatatag
     5461 agtttattga ttgtattgat ggtagaagga aattctgtca aattaaagct ggaccaaaca
     5521 ctattaataa agacgatgtt aaaactataa ttgaccactt tagctcagtt aaaagacttg
     5581 ctagaacaaa tggactttca ttatcttata atgacttaat tgttggtgtt ttttatggaa
     5641 cagaaaatga gcttaatgct cactataaaa gaattaatga agagtatcca gtagttgttg
     5701 gtcaagattt ttggtatagg cttactggtg atgaaaattt ttattttgat ttaatagata
     5761 caattgctca atctgcttta gaagaggact gtactgtttt acttaaaaaa acaatatcca
     5821 atctagctga tgaaatttct aaagataaat ctttattcaa tcaaaagtaa tataaaaatg
     5881 attaaatagc ataatttaca tttttagata tttaaaaata atattcacca aacaaaatta
     5941 ttaagtaaaa tgaaatgtat cttataaaag ctgagctata agtaaatctt tgtaaaaaca
     6001 aatttactta tagctttttt atgttcaaaa aacacaaatt atatactatt taatatatta
     6061 taaactttta tttgttaata ctctctacta aatttctaac atctcttata taaattaact
     6121 atttaataat tcaatcataa atctagctcc tgccttaaaa ccaagcttat aaccaagctc
     6181 tctttcaatc tctataactg aattaaataa atcatttaac tctatcaata catccatttt
     6241 cttttcttct gataaatttt tttctagtaa tttattaact ttcttaattt ctttacatct
     6301 atcattataa tactcatctt tttctagtac tttcctatac atgtgttcat ttatccaata
     6361 atcaaaatat atttctataa tttcatcata catgttagta cctccttgac tttcatctgg
     6421 tacttaatat atacttaact tgtgtagatt agtgtacatt aagtacctaa tttataaagg
     6481 tagttgttat atttatatgg caactacctt tttctaataa atttttcaat ctaaatacta
     6541 tatattttaa atcttcattg cttactattg aggattatat acttttcttc taacactcct
     6601 taaacaatag tttatatatc acttaataaa ttatattttt tcatacctcc tttaccttct
     6661 tacattcctt aaaagaaaac aaacttccta tataccaata aggtctaaag cctgattccc
     6721 catctctatt tttagcaact ataatttcac ttaaattagc tccaaaacct ttttctctac
     6781 gtcttaattc ttttatatcc tctattccca ttgtcttaca ataatcaaga gcctctttct
     6841 ctgtaggtct atgaatatat atgactgaat tagaatccat atatatctgt tttgaacttc
     6901 tcattactct atctcctttt ggcctttcat ccttcaattc atcatttaat tgagccaact
     6961 gtataattgg aatattaaag tctaaagtaa tcttttttat ttgccttgat aaatcagcaa
     7021 acttagcttc tccaacttct ttaccttcgt aatccaaaat ctgaagataa tcaattataa
     7081 caacatctgg tttaatactt tttatttctc tcttaattcc tgatattgta ttgactgaat
     7141 cattaatata gagattattt tcctgattta aagtacttaa agcatttatg tatttaatta
     7201 tttcttcttc atttagattt ctattcttca tttttacaac tgatattcca gttaaagaag
     7261 ctatgtttct aaacaaaatt tgtatatcac tcatttcccc acttataaaa agtacttttt
     7321 taccttgaaa taccacattt ctcattatct gtaaggctag tgctgttttt cctcttccac
     7381 ttttggctgc tattgtaatt aattcaccac tatatagacc tccaatagtt tcatctaaaa
     7441 attttattcc tatatcgata ccttcttcaa tttcatcatt tatgtattca aaggctcttt
     7501 ctgatatagc tgttatacta gaatccttct taaccaattt ctgattaact tcattaacgt
     7561 atttttctaa ttgatatata agtgtattat catctatttc acctaatttg aatttattaa
     7621 atttatcaat tatatttcta tttttatatt tttcaataac tatctcaata taatgggata
     7681 cattacctat agttggaact attgttgata gttttgtcaa ataacttata cctccaacat
     7741 aatctataag cccctttttc tttaaatctt ctgtaagagt tattatatct ataggctcat
     7801 cccttttatg tatatctttc actgaattga atatatgttt gtgctcttga tgataaaaca
     7861 tatcttcact tattaatatc tcaatctctt ctattttcct agaatctagt aaaatactac
     7921 ctaatataga ttgttctgct tctgtgttct taactaaatt actcatatta tccataatat
     7981 aaactcccta ccactttaat tgtgaatcat ctggactata tgcttctgca tctactttga
     8041 cattaattga tttatcttga taattgcctt ctaatatctt aataaaatta tttgggctca
     8101 ttatccaatc aaaatttgcc ttccaacctt tttcattgct tccatttaaa aagctagttt
     8161 tactaactat atgaataatt tctaatacag tctctttatc atattctttt attctagcat
     8221 taattacttt ttttctagct tctgtaagtt tctttacttt tgatagttct gtacaagtaa
     8281 tattgaattt ttctataatt tcactgtata ttttactttt ttcattattt acattcttgt
     8341 tagttgttag ttgcttgtta attgcatgtt gattagtctg ttgattttct tcattagatt
     8401 gatatagctc ccagttttca atagttataa gcctattttt gtttgttgat ttatttgtta
     8461 gaaatccata tctttcaaat ttctttaaag cagttcttac attctgaata cttacatctt
     8521 ttgaacacat atttgctata ctttccaaag atgttattaa ctgtcctgat ttaacttcat
     8581 attctttacc tccataaacc catttattag gcttatgatt aactgacagt aataggttta
     8641 tgagtatatc tctttgtttg gaattacaat ctttatatag tgggctttct tttatctttc
     8701 tatgaagctt tatccaacct agattactca aatactatca cccacttaag tctataattt
     8761 tataccattt gcattttcaa taaaccaatt atctagttta ttttttatta tatacactct
     8821 attatctatc catatacaag gaaatccttt atttttagaa tgacataact gtctaaattt
     8881 tgattcccct atgggataaa ttttagaagc ttctttacaa gtgataagtt gagctttatc
     8941 tcttctatat gtctgtatct ccttgtaccc tttttccttt tcttgtgctt cttgttcttg
     9001 cttgacaagt atattcaatg tagcttgtag tgttttaaga acttctagat tatttgtatc
     9061 tgccatacat cattcctcct tatcaaaaaa taattcttct acacttgtat ctagttcttg
     9121 agccaatttt aacatcaaca tattacttgg attttttgat ttacctcttt ctaaagaaga
     9181 taaataatat cttgagatgc caactttttc agctaattca tattgcttta tacctttttc
     9241 aattcttttt aatttgagtt tcattgattt cctcctttct ttcataccca ttttgtgtat
     9301 cctcatttct ataaaatatt ctaacaccca tttggtgtat tgtcaatata tttaagtgta
     9361 tcaatagacc catttagtgt tataatgtat ttgaggtgaa attatgatag ataatacaat
     9421 tggaaaaaag attaaacaat atagaaaatc tataaatatg tcaagggatg agttagctaa
     9481 taagattggt tgctcagttt atgctgtagc taaatatgag caaggccaaa gaaagcctag
     9541 tttagatatg ataaaaaaaa tatctactgc attatcaatt cctataaata ggcttattat
     9601 tgatgaaagt gatttagata cttcaacaaa taattatact aatttaaaag aattggattt
     9661 agttgtaaaa acatttgata attactcagc aaaactcatc ggaaataata taagcaaatt
     9721 tagaaatata aataatatat ctctcaaaga actctcagaa aaaataaatt gttctgagaa
     9781 tgaaattata aattatgaga atggaacaaa aatgcctaga attgaaatat taaaaaaaat
     9841 agcacttgct acttatacta agattgaaga cttatttgga tttccattag aagagataaa
     9901 aatagataac caaacaatcc tctatagaaa tccccagact ggatatatta tagataactt
     9961 tcttatcgat tttataaata aagatattaa catagataat actccagttt cttattatat
    10021 agataagtca gaatttgatt tagtaattca tatattaaaa aattatggtt atgatgtaaa
    10081 aacagattat aagacaaagt ttgaaattgc agataatgca gatacaataa aaccaattag
    10141 cttaagcttc tctgacttca aagacttctc caaaaatcta aattgggtga taaatggtct
    10201 tgttgaaaat tttatacaag aaaactctaa tggtcttatt gaagatgagg atgaagaata
    10261 ttagtattgc acattttcaa aatatgagtt tacctattaa cttttataca ttcatagtta
    10321 ataaataaaa attagatatg atatgaaagg agctaaaatc atgaataaat catatatacc
    10381 aaaatacaca cataaattcc ctactaatct acctcatgga gataaaaaat atactttaac
    10441 atacattaga agaatgataa gtgaatttgt atatgatatg ggtaacctag aaaataaccc
    10501 ctttacattc ccagaagtac aaacattact tgatggtata accgtaggtg gacataaatt
    10561 aagtgaccaa aatcaaatac taaatataaa atcatcatgg gattatgtaa taaaattaag
    10621 taacaaagaa aacttatctt taaataaaga tacaatttgc tctatacata aaatagttgc
    10681 aaaagatgaa gctctaatag ttggagattt tagaaatggt aacataggta tagctggtac
    10741 tactcaatat gaatgcatag aagccactct attaaatgat ttatttgtaa gcgacataaa
    10801 tatcataaat aaaataacta accccttaga aaaggctata ataataaatt tatggttgtc
    10861 ctattgccaa ttcttctatg atggcaacaa aagaactgct agacttacct caaacctaat
    10921 acttataagt aatgatatag gagttctatc aataccagct aaacataaac aagaatacaa
    10981 cacattaatg ctaaactttt atgaaacact agaagctgat gaagttataa aattcttatt
    11041 agagaaatgt attacattct ttgatgaata taactataaa tcatataagg agctgtttaa
    11101 aaatggcaac taaaagagct aatggagaag gctctatcgg aaaatataaa gatggttggc
    11161 gttcccgtat aatgattggc tataatgaag atggtaaacc aataagaaaa gaattttatg
    11221 gtaagactca aaaggaagtt aaagataaac ttgatgctta taaaaaacaa tattatttaa
    11281 gctctgatat ttcagatgat aaaataactt tagagcattg gttttacaat tggttatttg
    11341 aatatagaat aaaagattta aaacctaaat catttgaaag atatgaaagc atctatcgta
    11401 attatattaa agatactgat ttaggcaatg ttaaattaaa ggacttaaga gtatctcaca
    11461 tacaaaaata ttacaataga ttgcttgatt ccaataaaag tatacctact ataaggcaaa
    11521 taaacactaa actgaaaacc tgcttatcag aagctgaaaa acaaggtctt atacaacgta
    11581 attattgtac tatggtaaat ataccagttg agaaaaagga gaataagctt gaaatattga
    11641 ctcttgaaca acaaaagagt tttataaaag ctatagatgg acataaatta gaggtattat
    11701 ttttacttgc tcttggtact ggacttagat taggagaatt attaggactt aaatggtttg
    11761 atattgattt taaaaagtct aatttaacag ttaaaagaac ccttcaaaga acatatttta
    11821 tagataaaac cggaaataga gagcttaaag tattagagca agagccaaag acatcaaatt
    11881 cttacagaac tgtacctatt ccaaaagatg ttttaaataa attgaaagag cataaagtaa
    11941 atcaaaattc ggatatatta aaggctggag aattatatag aaatgataat tatgtatttt
    12001 gcaatgaatt aggtgcccct attgatgata aaagaccttt aaggaatcta aaatctatat
    12061 taaacagctt agatatagag cctataaaat ttcatggtct taggaaaact tatgcgacta
    12121 gactttttga gaatgatgtc cctcctaaaa ctgtacaagt tttaatgggt cattatgata
    12181 tatcaataac tttaaatata tatactcaag ttatggaaga taagaaaatt gaagctgtag
    12241 aaaagttgga taaaatattt tcattgtaaa aatcaaatat ataaaaatac tctgatttca
    12301 tatagttaat tacaaaacca aaataaggat acaaattaat tttatattat cttgtatcct
    12361 tattcttgtc tagtttttta aatatttatc ttggatagcc tttatgatac attaatccat
    12421 tttctaacat tagttcttgg gctaaatata tatgaccctt acagtgcata tctgatatac
    12481 cctcacttat aagtttatat gtttcagaag tatataaata atccatactt tcttccaagc
    12541 tcttacctgt ttgtttagaa aattctaata tgattttttt atatagcgat tgtaacaatg
    12601 tcttattagc ttccataaaa gctatacctc ctctttcaaa tatttgttta tcctattttg
    12661 actaactata tatttattag ttatttaatt agccataata attcggctat aagatattta
    12721 taatctaatc tcaattatat aaatcttttt gccaatattt aataatcaaa atattgcact
    12781 taattaacta tcttataagt attgataata aaatctgtct tcttaaatat acttaaataa
    12841 aacttctata attatatcaa agtattaata ttcactctct attgtctctc ctacaagtct
    12901 attttctcca tttagggtat atactcctga tatagttggt tgtatgtatt ttctatccat
    12961 atatggttct tctaaaactc ttttttctgc tagcatcgtc caaaatacac tatatccttg
    13021 ttcattaaga tattttaaga aaatttcctt attaataaag aatcctatct cttcattaag
    13081 taattcacta ctttcaaaac aaacaacttc accataatca ttatatagaa tcgaatctcc
    13141 ctgaccatat ctcaatttca acccataaaa tagagtacta caaggtttat gccatgtgcc
    13201 agaaaagtct tcttgaaata tgtcaccttc tcgttcagta gtataactat aagttggcaa
    13261 taatacttta taatcctttt ctaagatatt cttatgttca tctatttgta cccactcttc
    13321 tccacaataa tatgaatttt gaaaaaagta atatccatct gcccaataat attccttatt
    13381 ataaagagta taattactat taggttcagt aagccatcta cccataaagt cttttccctt
    13441 tagagcattc actattggtt ctagttggtc atttttaatt atatagctat tgattaatac
    13501 ccacatgtct ttttcaggat ttttatagtt cttattttta aaatcatttt cctccttcca
    13561 agtataccat ccattaagta ataaaaagtc ttgatttttg taaacactat ttaccatatc
    13621 atttatgctt ggtaaatcac taaaatctcc taaccactca ttattaggta ttggagaaaa
    13681 ttgaaataat tgattatgta ctcttttatt gttattatta tttattactg tcatgacagt
    13741 tggatcaata ttcctaatat ctggctcaaa tgtaccctta caataaattt cttttttagt
    13801 accataagca tctgtataca cttctatctt ataattatca gcagcttgcg ctgcaagttc
    13861 atataatgca atccattgat atttctttcc tattctttca tatttactat ctcttaatct
    13921 accattttgt actgttctat caaatttacc atgtttattt acatcatatc ccatatcaaa
    13981 aactttctta atagctatat tctttaaatc gttatagtct agacctttcc atttatggaa
    14041 atagtgttgg aacatatatc ttccaaaatc tccataccat cctggttgtc catctctagt
    14101 atattctact tccatagaat ttaagatagt gttttgactc caataataat ctttaaaatc
    14161 tgatgcctta tagtcatact tatatccctc aatttcttca tcagttggta ctttaggaaa
    14221 aattgattta tatggtggtt caacattcat atcttttaat actgaactat caattttata
    14281 ttttgcataa tctacaatat tttttgcata atctcttaat aaaatattgg gataaacata
    14341 atctacatca aatattttat tataaacata aactgccaat gcctctatca tctcttctga
    14401 ttgctctgat acaacacatc caaatgcaac tgcatataat cgttcaataa tatatgggtc
    14461 atctacatct tcaaatgttt taagtaaatc tattacaata tccattttcc ctgttaatag
    14521 atttacaaga gcttttgtac ttttatctct taagtctcta tttgaagaaa ttaaaaacgt
    14581 tgcaatcatc atagctgaca atctaattgt ttcatttgaa gtattatcaa aaccttcaaa
    14641 atcaaagcac catcctaata atcgcttaat agaagactct tcttcttcat aaacttcatt
    14701 aaatataggt ataaagatag catctctatc tgccatagta ttttgtaaaa tatattttac
    14761 tatataatct gcatttaatg tatgattaac ctttgtagaa attaagataa gtacattata
    14821 aaaatctatt ttataataag atttacctaa aactatttta ttaatataat ctagtgtttt
    14881 ttcagtaatt gtagatttct tacgccattt tagtgcattg ataaatgcac taataacatt
    14941 ataatttgtt tctttatttt taaaaacttc aaaaatttca ttaggtatat ctgagcctgt
    15001 ctcttcagat aaaacaattg ccaatacttc taatatatcc tcatattcta ctaaatattt
    15061 atacttatgt gaaaaatcta ctaccccaat ttcttttaac tctttaacca acaaatttgc
    15121 atataaataa tcttctaact tctcatatgt cacatgcata tattcttcat catttaaatt
    15181 gactgtttta ttaactattc catttgctat gagttcatct attatcacat tgttaatctt
    15241 atacttactt gcaatattta ttataatctc tacactcttg tttgcgggta ttaaattatt
    15301 cccaagacca tctccatatt tatatttaat catttcatta agtacttctg taactacatt
    15361 ggcatgtcta ctataattgc atgattcaga tattctaaga ttaagtatat tcaaataatt
    15421 tttataaata tctgtaaaac taatagtatc taagttaatc tcttctcctt taaaagtttc
    15481 acacataatt ctcaaaaaaa gagggtttct aaattcttga tttggcattg gaatattaga
    15541 ataaggtatt ccataataat caaaatactt tttcatagct ttatgttcta tactagaaaa
    15601 tccttgatgc tctataaaaa ccaaattctc ttttatcttg ttattattat ctattaaaga
    15661 tggtatatat tcacttctta ttgataatat tagtccaatc catggatatt tctttatctg
    15721 ttctacaata ccatttaaat aatatttcca aagttctttt ccttgacctt cattcaatgc
    15781 atctataaat aatatcacac gctgttgctc tttttctcct atttcattaa gtatttctaa
    15841 taattcttca aactcacact caatttctaa tatttctaac atatgtttaa ttggtgtttt
    15901 atccaactta aaatgttgcc ctaaaaataa tatagactta tgcccatcca tatttctttt
    15961 ctcaatagta tgtgcaatta tatgagattt ccccatacca cctgcactat gtaataaaat
    16021 ataagggcta ttaactactt ttatttcctt agaatccaaa tattttatat atacatagat
    16081 atctttttga gtctgcttca atttgtatat ttcatcttct ctttttttac ttttttcaca
    16141 gtcttcattt ataattatat ttttaaaatt atttattcta tcatcaagtg ctgcacttat
    16201 tttttcaaag tgctttttta acaacttgat atctaatctt tcattagctt tgtcataaaa
    16261 ttgttgtatt atttcttcta taactagtac atctacttcc acttcattta ttttctgttt
    16321 ctttatactt gatagaactt tatctgtttt ttcatcaaaa cgttgtttaa attcttcatt
    16381 ttttcctaat gcattaaacg tattttctaa actcaattct acattcaatt ctggtaaata
    16441 tctatctcca aggtttttta tctgtatatt attctgattt tgaaaccatt tctctgttaa
    16501 tctacaagat aaattagtta acctactttc ctttaaagac ctatccttta atcttctata
    16561 ttcatctggc aaaactattt ccaaaattcg ttcaagtatt attcttacac actcatgctt
    16621 accttcagga tacataatgg tttgtatata atactcattt tctaactcta ttcgtctatg
    16681 tttctttttc tcactactaa ctggaataat agcataatga cccgttcctg cctctgatac
    16741 ttgctgtaat aattccaacg gtctatcctt ttccaaacta catccaagaa ataacaactc
    16801 tttacttgta tatacttgtg taagcatctt tctcaattca tcattactat atgctttttc
    16861 atattgttct tttgttaaca caatagatgt tgtaggctct gtaatatccc catgaatttt
    16921 atacaatact aattcattat ttctaagtgc attatttaat gcttcaaagt gacctggatg
    16981 tgacactaaa actgttttat tgtgtaatcc ataatttttt tctagtactt tatcataatt
    17041 agtagttata attaatcctg agccaaaaat tataggaatt aaatagacac atttatcaat
    17101 aaaatcgtct ttatcttttg catccagata acctgcccca aaaacttttt ttaaatgttt
    17161 agaaaatcta actttcgcca attcttcata taaaaattct gcttttttct cataatcctc
    17221 tattttttca aataaaattt tatcctcatc ttctaaatat tcttcacttg tgttatttaa
    17281 aaatgtactc catgaaggat atatgtcttt aaataacata gacatgcctg ctccaatata
    17341 tgggactaca taaccatctt taattgcatt taccagttca tcaaaatttt tttgattgta
    17401 aggcttatta aattccataa tttcttcaaa tgtcattatt ccctcctaaa ctatctctta
    17461 ataatttcaa ccataattcc tattttataa ttttattata tcattccatt ccattttttt
    17521 actacaattc tatgttatag aatgaattca cttacttaca tttgttaata atataaaact
    17581 ttcttttaag ataattaaaa tttatacttt taaattatgg ataaaaatgg aatgttataa
    17641 cttttaatgt attactaata ttaacaattt taaaaaataa tactattctt attttatgct
    17701 ataattatat tttgcataaa tattattgat attttataaa tataattata cttatatctc
    17761 atactcaaat attttatata atatataagt ctaaattaac ttgcaatata aaagcgcctc
    17821 ttaatatagg cgctataatg ttataaattt ttaattatat attccaatga acctttatca
    17881 accatatcta aattaaatat ataatccatc ttatcaaatg catctcttaa tggaatactt
    17941 gattcaagcc atcctaaccc atctgtaatc caacaaaatg taaaacctcc atcttttata
    18001 tacttatcta acttagtaaa ctcatttgca acagatttta acttagaacc tccaccatta
    18061 taaaaattaa cttctactaa aacaattttt ccattattat ttatagcaaa gtcatacttt
    18121 cttttactct tatctgttgg aacttctata gaccatttct cacttatttt tttcactgta
    18181 gcttgtgtta aataactcca attattcttt tcacacatat tctttacata tctttctaca
    18241 agagtttcca tactacttcc agttctattt tttctagcat tcgtatcaag cccaacctct
    18301 actccaaaca cataatctac taaatttttt atattttttt gttttaataa atctaaaaac
    18361 tttatttttt taagaaattc tgtagctaat tcgctttctt catcacttat actctttctc
    18421 ttcttaaaat taaacagttt atatttaaac atatcctcat ctgatggatc tagtattttg
    18481 aattcattat ctctactcgc tattactatt ggtatcacac taagaacttc tggatactct
    18541 tttaataaat ttttagtttc gttttctata tcttcttttc ctataagata gtttaagata
    18601 tttaactgaa cctctatctt tctagtattt tccaaagctt tattccagtt aacataataa
    18661 tcccagctag ctatagtacc ttttaaattt tcaagtaatt ccttaaatat ttcatcctta
    18721 ctcttatttt tatgaatatt cttaaccaat tccatttgat tccccacctc ttcataatta
    18781 gtaattagca attcagttat aaaacctctt ccattcccct tagaatttat gaatctttta
    18841 gcatgtactc tttttatatt aaaatctgaa tacaaatcat caaagaaagt atcttcttca
    18901 tctaaaactt ttggatcaga attacttaac atcaattttg tatctatttt atctagaact
    18961 ttataaaatt tacttaactc aatttgacta tcatcattaa aattaccttt gttataggaa
    19021 ttaaatcctc ctatagttac tggtctatat ggtggatcta gatacacaaa acaattttta
    19081 tctattatat tttctagatt taaataattc ttatttaata tttttacttt tagctctcct
    19141 cgagaatctc ttttatttaa aagcatggat aatttttcaa gttcattttt atcaaaaaaa
    19201 ctaggatttt tatatcttcc aaaaggtaca ttaaattcac ctcgagaatt ttctctatat
    19261 aatccattaa aacatgtttt atttaaaaat ataaaatatg aagcttgctt tatacaatct
    19321 tcattttcta tatttttttc atttgtaagt atattaaatc tatctctaat atcatagtac
    19381 atcttttctc tatctgaaga accttcttcg taagatatgt attgttcttt taaattagtt
    19441 agttctgtta ttaactcttc caaatcatct ctgactttaa tatataaatt aattaactta
    19501 ctatttatat cattaattac atacttgttg aaaatgtcct cttctaacaa atatagaaat
    19561 agtgcacctg cacctacaaa aggttctata taagtcatgg aactaaaatc acaacctgta
    19621 ggcatttgtt ttcttagaat atcaagtagc tgtgttttac ctccagccca ttttaaaatt
    19681 ggtttcatgc aacacctctc tcagttatat tgttatataa ttatataaaa taagattcta
    19741 aaatacaagt actcatcaga acactatatt tatatcaaat ctttccaaaa tctaagtatt
    19801 attttatcct aacacttttc aaaacctcta aataatatac aatattctct aatatttact
    19861 tactaaaaat ttaaatctat ctttaagtat aacctaaaac agattataag ttacgtagtt
    19921 tttaatataa aatatacttc tacaagtaaa gctttaaata tcaattactt atttcataat
    19981 taatgactat gtaccctcaa aaaaacctca ttttctatgg ttggggtacg gttggtggat
    20041 ttcatcacca aaataaaaaa tactagtcaa taaagactag tatttccaag tatttcatca
    20101 ttcttttttc aaaattggtg gagacgaggg gagtcgaacc cctgtccgca aatcttttcc
    20161 ttgctgcatc tacatgctta gccaacttca ctaatatctc acctcacata gcgccaagtg
    20221 gcaggctcta tgcttagcca gtcccaatta atctacttca ggtcgagaca cccctaaagt
    20281 agttccctgc ataatgacgt ccagtcttag tcggcaggag gactaagagg gacgagcagc
    20341 aactaggctg ctatcgcgaa attatcgtct gcgtttattt taaagttccc cagtttttac
    20401 gtgacccaga gtaacacgac atgcagcctc aagttccaaa cccacgtcga aacctttacg
    20461 cccccttgag tagtttatag tccagtagac ttttcgcaaa ctttatcacc atttttatta
    20521 aatttttaat gtatatataa tataatactt ttaagaataa aaatcaatgt tatgttctag
    20581 ataataaaga attttattct aaaaaataca gttttatcta gtttaatact tacctgtcat
    20641 ttctctttct atacgtcttt gtgcatcttt cttagccata tcttgacgtt tatcatatag
    20701 ttttttacct tttgctaagg caatctctac tttaacttta ccgtttttta gatacaaact
    20761 aagtggtata agtgaatacc cctttatagt agttgctcca ataagctttc ttatttcatg
    20821 cttatgaagt aatagttttc taactcttaa agaatcaaca ttgaatatat ttccttgctc
    20881 atatggactt atatgaactt gttttaaaaa tacttcgcta ttatctacag atgcaaatcc
    20941 atcacttagg ttaactttgc cttgtcttat tgattttact tctgttcctt ttaattcaat
    21001 tccacattca taagtttctt ctataaaata ttcatgtctt gctcttttgt ttgttgctaa
    21061 tattttttta ccagccatat aaatctcacc ttctttgcta gaaatttatt ccataaatat
    21121 tagaaattta ctaaatattt aatgttgcca aattatttga ctaacttgta tgataacata
    21181 tttaggcatt ttagtcaata tttcactgat atattaagtt agttaggcta tatataatta
    21241 agtaatataa atgtatgaaa atagctaaaa aactacttcg aatatactta atactaaact
    21301 aactctaacc agtaatacta atttataaat ttcaaaacaa aaatattaca tattataaat
    21361 cttctatctt ctatcttcta tcttctatct tctatcttct atcttctatc ttctatcttc
    21421 tatcttctat cttctatctt ctatcttcta aataatttta acatgcccat aaaatataat
    21481 acccacaaat gataaataat actcaaaatg tctattgaaa taactataca tccttaaata
    21541 acttaactta aatctaaaca tttcaaaaac ttcttaaaaa tatgtaaaaa tttgatgata
    21601 aagtagcgat tttttcaaaa aaaatgtaat atagagttat atcttataat attttagaga
    21661 taaattttat catagtgggg agaaatttgt atggatttaa gcaaaaaagt aaaaatttta
    21721 gaaaatgcat cagatggcaa aagcgaattt gagatattag aatttgaaaa acttgaggga
    21781 gcttctagcc cattaatggc aatgggatta tattttgcta gagaagctgg tttgaaagct
    21841 agacaaatta agataaaatt aaataattca tcaataaaaa cagaagctgg tgcattatac
    21901 tattataaag gaaatatatc ttctgaaact aagattggtg gtgttggagg tctttttaaa
    21961 aaaggaatat caggagcact tactaatgaa tcaatgatga aacctgtata caaaggtact
    22021 ggagaagttt atttggaacc ttcttttaga cattatttat ttatgagtct tgataatgat
    22081 tctataattg ttgataaagg attattctat tgttgctctg atacaataga agtaaaagca
    22141 tttacacaaa agaacttctc atctgcttta cttggtggtg aaggcttctt ccaaatagaa
    22201 ttaagtggta ctggtgtaat cgttctggaa tgtgttgtac ctcaaagtga aattgtagag
    22261 tatgaattga aaaatggtga agaattaaag gtagatggta actttgcaat agcaagaaca
    22321 tctggtgtta atttttctgt tacaaaatca gataaatctt taattggttc tgctttaaat
    22381 ggtgaaggtt tcttaaatac atttactggt aatggtacag tttggctagc tccaactcaa
    22441 gtaatgtatg aaaaactaag ttttggagca atgcctacta atgataatat gaataataaa
    22501 gtttctaagc aaaaatagtt gttttataaa tatttattaa atcattattg attttataaa
    22561 gtcactataa attttattag attattatta taaaatcaga gtgtggaaat gtttatgtgt
    22621 attctttaaa taaaaatttc cacattctgt aaggttatta tacttgatat ataaaattca
    22681 atttaggaac atagtctaac atttataaac atattaaggt aatgatttct cttggtaatt
    22741 attctcatat cttattcttt gattatattt atcttattcc tagcccttat gtcatattat
    22801 atttcttatc ttattactta taattagcat taacttatct aaaatcttgt atttttttaa
    22861 tagtaaagtt tacatctata ttaaaaagtt tacatctata ttaatttcat aaaacaatca
    22921 aggtagtcaa gagattcaac tttacttgat tttaaaaatt taagtgaatt tgttcttata
    22981 cacatctttt taaaattata tctgtagaaa atatcattta gagctataat taaattaact
    23041 tattaaattt tagaaaattt attaaaaaat ataagttaaa ttaatatttt taaagattct
    23101 tttttgtaac tactagaata gtaatttagt agttattgta tctctaaagt ttaagcatgg
    23161 atagatatac atttgataat ttgcctataa tttacctact acttggatta ttggagcaaa
    23221 tacaatatat taataataag ttcctaaaac caataaatta actaatacac ataaagatat
    23281 tgatgttgat tatacaacta taagaaacgg atttaacaga atttaatcaa gctaagaaat
    23341 tatatatata tcgtgatgtt tttgaattag aaggtaattc aaaattagtt tatagtcttc
    23401 tgtcaatcca ataaaaagta cagggtacaa gaattgttag taaaattttt aaagatttta
    23461 tggataaata taaatagttt tacttatttt ctaaaacttt aaaataccat taattacctt
    23521 aaatatttta ataggaggac atatgaattt aaataaagtt aaatttactg aagaacatgc
    23581 tattgaagtt actaattgga aatatgaagg taaatattca atatacaatc tacctccatg
    23641 ggaggaaatt aaaaagaaga atttttcact agcaaaagaa gataaaagaa aaaattttat
    23701 aagttttata aatgacaata aagaattgat aggttttata aacttattag atgaaggaag
    23761 ctctgtattc tttggtattg gtatgaaacc gaattattgt ggaatgggaa ttggtaaaga
    23821 aataattggc ctaggattac aagaatgtag aaataaatat agtactaaac caattgtatt
    23881 aaatgtaaga acctggaaca tgagagctgt aaaatgttat gagtctcaag gatttaaaat
    23941 agttgagact aaggtacaaa aaacgcattt aggagatgga gaattttttg ttatgaggta
    24001 tcaatagttt ataacaatag tcttattgtt aatacaacaa ttaaaatgaa aaaatacata
    24061 tgtgtaggtc atagcaataa gtgtctttga aatataaatt tgagagcttt tgataaaatt
    24121 ttgtatacat atatatttta tactacggat atataaatct atctacatat ataaagagat
    24181 tgtaagtagg tttgtgtata taaaaataga tttatatttc ataaatgtac tttttatttg
    24241 tattaccttt attttatcta agctctcatt ttattatcta atcttccttt ttactggttt
    24301 tattaagaga gatgcaatta tcaacacaac acttccaagt atcagccaat atttctcatc
    24361 aaaactacct tgactcatag acattataaa aaattttcca acatacttgc ctttagtcat
    24421 catatttaaa attaaatatc ctatagatat catataacct atgattgtat tattaaataa
    24481 atttgagaaa aacattccta aacttcctat aaacatagct gatgcgaaac ttccaataat
    24541 atactcaact actggaaaat tacatccaag atatattaaa acatttacaa aacctatgat
    24601 taataccact atacctataa ttgacatcag tatcctaatt aattgtacaa taacttgaga
    24661 taatgctttt gaagatacta tctcatcaat ttcttttgat tgctctagcg aaaatacagg
    24721 taccaacatt actatcccta ttattgcaac ataattttct aaacatatag ctgttgaaat
    24781 tctgtctaaa ttagctactc cttgtattag aggaaccata catagcaata taattgaaca
    24841 tattaaatta gttaaagtgc ttaatttgaa gttttctttt attagaacta aacagttttg
    24901 aaaactttgc ccaaacatca agattccccc ttcttttttg ctcatataag aatatagcta
    24961 acaaaacaag caccaatgct cccaagctta tcacaattct attaatcata atttgattga
    25021 agttttgtat aataatatcc ccatttaata cactattatg tcttatcatt agccccattg
    25081 ttgaataatc acctttgagt cctgtagaat ttatactgta aagccaccaa attaattgca
    25141 caataattgc tactggtgta tctgttaata tagtaatact cattcctaca gctgtaacta
    25201 tcataagagt tggtagtatc caccataatg tatattttat aaatgctaag ttatcaatag
    25261 atataccttt atctttagca aagaaaatca aaggaagtaa atctttcata cctaaaatta
    25321 atacaggcaa tatcatcata aagacaagtg caaaatatct tgataaaact attttgcatg
    25381 aagatacttg ttttgaataa ataatctctc tcattcttga acgcttatct ttaagtgaca
    25441 taaaaactgc cacaaatact ggaaatatac ttaaaactat acctatataa tcgcaaaaaa
    25501 ttcttgcata cccattacta actttatctt tttgtattgt atagttgtaa ttcttcatag
    25561 cttcatcata ggtaactgga actactccaa atctttgtaa cttgtcaaaa gcatatgcag
    25621 accctcctcc taaaatgtcg tctactcttt gcattatttc ttcaaatctt tcaagagata
    25681 ctttagaata atctattttt atactttcat cagttttcca aagtgaattt atttcgctaa
    25741 tacttttacc agttatttct tctatatatt tttctatggt cttcgtttta ttttggtcta
    25801 attttatata cttaactacc cctgttggat acgttgtata tgtgtttttt tgatattctc
    25861 taattaggtt tatagttgca tttggaatga ccttttctgt tttcatagat tctttttgac
    25921 catatggata ttgattgtgt acagaataat attcctcaat attctctact ttttgtggag
    25981 caactaatgg atttccatca ttaggaatat ttttacttac atcatttgaa actgaacttc
    26041 caagttgaga aatatagaaa aaaactaaaa ctacaataaa aatataatat accatactct
    26101 ttaatatatt tttacattct tttataaata gattcatata ttatctcctc ccattataag
    26161 catatagcca tcttcaatag ttggattaca atttactgct gtctcaaaag gtttatcatc
    26221 tgatataatc ctaagtatag cctttccacc atgagtcaac attcctataa ctataaatct
    26281 actttttata ttttcaatgt cttttttatc tacttcaata ctatatactt tcccttcagc
    26341 tagttttgct aatgcttctg ttgagccatt gtatatgact tttccactat tcaatatagc
    26401 tacgttttca catgtagctt ctatatctcc aacaatatgt gtagataaaa taacgattct
    26461 atcatttgca atttcgcata gtaaatttct aaatcttata cgttcttctg ggtcaagacc
    26521 tgctgtcggc tcatctacta ttaaaactct gggattatga attaatgctt gtgctatccc
    26581 aagccttcta ttcatacctc cagataaaga ttttactttc acattttgat aattagtaag
    26641 gtttactttg cttaataact ttgctataat ttttcttctt tttcttagtg gtatatccga
    26701 taaaactgct aaataatcca ttgctttata tactgtcatg ttaggataca ttgaaaaatc
    26761 ttgtggcaaa taaccaacca tatttctaat ttccttagta ttctcaatag gaactccaca
    26821 catatgtatt gaaccttctg tttttgataa taaagtggct aaaattctca taaatgtggt
    26881 ttttcctgct ccatttcttc ctaaaagacc aaacatacca ctgtcgattt caagatttat
    26941 attatcaaga gcttgttttt tcccaaatct tttactaaga ttggatactg ctattttcat
    27001 ttccatatat aaatacaccc ccagaaacaa ttttatagaa ttttattttt ataaattttt
    27061 actgtatttt cataataaat tctaaaactt aagatattat ttataaattt ctaaattttt
    27121 tctaaagatt ttaatttttc tgtttttaat taaattaaag tatctttgtt tctgaaattt
    27181 tttaagttta tgaagttata atttaatata tatttatttt atttgtgagt ctatttttat
    27241 at
//
LOCUS       DAMJYX010000045        25972 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJYX010000045 DAMJYX010000000
VERSION     DAMJYX010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.045"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            164..1039
                     /locus_tag="PC479_002002"
     CDS             164..1039
                     /locus_tag="PC479_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2804546.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1057..2547
                     /gene="cls"
                     /locus_tag="PC479_002003"
     CDS             1057..2547
                     /gene="cls"
                     /locus_tag="PC479_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2804547.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2740..3024
                     /locus_tag="PC479_002004"
     CDS             2740..3024
                     /locus_tag="PC479_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2804548.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3057..4685
                     /gene="groL"
                     /locus_tag="PC479_002005"
     CDS             3057..4685
                     /gene="groL"
                     /locus_tag="PC479_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2804549.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4947..5687
                     /gene="larB"
                     /locus_tag="PC479_002006"
     CDS             4947..5687
                     /gene="larB"
                     /locus_tag="PC479_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2804550.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5771..6505
                     /gene="larC"
                     /locus_tag="PC479_002007"
     CDS             5771..6505
                     /gene="larC"
                     /locus_tag="PC479_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2804551.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6535..6954
                     /locus_tag="PC479_002008"
     CDS             6535..6954
                     /locus_tag="PC479_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2804552.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCSES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7014..7115
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7336..8871
                     /gene="guaA"
                     /locus_tag="PC479_002009"
     CDS             7336..8871
                     /gene="guaA"
                     /locus_tag="PC479_002009"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2804553.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9410..10840
                     /locus_tag="PC479_002010"
     CDS             9410..10840
                     /locus_tag="PC479_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2804554.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFLLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(10988..11338)
                     /locus_tag="PC479_002011"
                     /pseudo
     CDS             complement(10988..11338)
                     /locus_tag="PC479_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11333..11854
                     /locus_tag="PC479_002012"
     CDS             11333..11854
                     /locus_tag="PC479_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2804555.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(11983..12219)
                     /locus_tag="PC479_002013"
     CDS             complement(11983..12219)
                     /locus_tag="PC479_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804556.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(12529..15957)
                     /gene="uvrA"
                     /locus_tag="PC479_002014"
     CDS             complement(12529..15957)
                     /gene="uvrA"
                     /locus_tag="PC479_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2804557.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            16276..18510
                     /locus_tag="PC479_002015"
     CDS             16276..18510
                     /locus_tag="PC479_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2804558.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            18766..20796
                     /locus_tag="PC479_002016"
     CDS             18766..20796
                     /locus_tag="PC479_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2804559.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            20793..21245
                     /locus_tag="PC479_002017"
     CDS             20793..21245
                     /locus_tag="PC479_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2804560.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            21247..22302
                     /locus_tag="PC479_002018"
     CDS             21247..22302
                     /locus_tag="PC479_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2804561.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            22369..22677
                     /locus_tag="PC479_002019"
     CDS             22369..22677
                     /locus_tag="PC479_002019"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2804562.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            22781..24046
                     /locus_tag="PC479_002020"
     CDS             22781..24046
                     /locus_tag="PC479_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2804563.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            24134..24850
                     /locus_tag="PC479_002021"
     CDS             24134..24850
                     /locus_tag="PC479_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2804564.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(25045..25338)
                     /locus_tag="PC479_002022"
     CDS             complement(25045..25338)
                     /locus_tag="PC479_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2804565.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(25344..25559)
                     /locus_tag="PC479_002023"
     CDS             complement(25344..25559)
                     /locus_tag="PC479_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804566.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         9858 a         2863 c         4519 g         8732 t
ORIGIN      
        1 ttaaggtaaa aatttaaagt aaaatattat tttacataat aataacctta taaattttga
       61 aattacttat ctttgtgtta aaataagtaa aatgcacata ttagagctaa gtttttaagt
      121 aaataaacaa aatgaaaata taaatattaa ggagatttgg tatatgaatg tttatgaaaa
      181 aggtaatgga gttatactag aaggagtaac agattttgac ccagtacata tatttgagtg
      241 tggtcaatgt tttagatggc acaaacaaga agatggctca tacactggag ttgcaaaggg
      301 aagaatatta aatgtcaaaa aagagaatga taaaatatat ttaaataata ctaatttaaa
      361 ggagtttaac agcatttggt ataattactt tgatttagga actgattata cagaaataaa
      421 gaataagcta aaaaatatgg atgaatattt aaataaggct actgaatttg gatggggaat
      481 aagaatatta agacaagatg gatgggaaat gcttatatca tttataattt cctctaataa
      541 taggattcct atgatacaaa gagcgataga aaatttatca agaaagtttg gtaagtatat
      601 aggtgaatat gaaggtaatg aatactatgc attcccaaca ccagaagaac taaataaggc
      661 ctcacaagag gaaataagag catgtcaaac aggttttaga gataagtaca taaaaagtac
      721 tacacaggca gttatagaga ataatgatga ggtatctgaa tatacaaatt taagtacaga
      781 ggattgtaga aaagagcttt taaaatttaa tggagtagga ccaaaagtat gtgattgtat
      841 tgcattattt ggtatgcaaa aatatgattc ttttccagtt gatgtttggg ttaagagagt
      901 tatgcaagaa ttttatatag atgaagacat gagtttacct aaaatgagaa cttatggaat
      961 cgataaattt aaagaaatgt ctggatttgc tcaacagtac ttattttact atgcaagaga
     1021 actcggtata ggaaaataaa ttaaggggga gaaattatgg gagtaatagg aacaatattt
     1081 ctgttctatt taattatatc atatctagct ggagccataa tttctgtgat tatattgctt
     1141 gaaaatagag accctgcaaa aacaatgtct tggctattga tgtttattat atttccaggt
     1201 gtaggtttaa tgatatatgc tatttcaggt agaaatatta gaaaaagaaa attatttaag
     1261 actcaaaaac tagctaataa tataaaagaa aagaaactat ttgatacatt agaaaagata
     1321 acagaaatag ttgaattaga aaaagaatct ataaaacaaa ataagctttt aagagatgaa
     1381 gaggatggta gttatagaaa aagggtaata aatatgttgt taaagactgg tatgtttccc
     1441 tttactaaaa ataataaagt ggatgtattt gtagatggaa atgaaaagtt taaaagactt
     1501 atagaagata taagagaagc taaagaccat attcatttag agtactttat catcaaagat
     1561 agtgaaatag gtagggtgtt aaaagaagaa cttataaaaa aggctaaaga aggcattaag
     1621 ataagaattt tatatgatga tgtaggatgc tggagatttt ggtttaatag aaaatttttt
     1681 cgagaaatga gggaagtagg tatagaaatt gcagcatttt tacctacaaa atttcctata
     1741 ataggaggga aattaaacta tagaaatcac agaaaaattg ttgttattga tggaataatt
     1801 ggatatacag gtggaataaa tataggagat gaatatttag gcaaaaatga taagtttgga
     1861 tattggagag atactcatat tagaattaag ggtatttctg tatacatgct tcaaatgaca
     1921 tttctgattg attggtatta tacgacaaaa gaagtattag tgactaaaaa ttatttccca
     1981 agtgtaggga atgttggtga aagtatgata caagttgttg cgagtggtcc agacagtgac
     2041 tgggaagata ttcattatgc ttatttttct gcaatatgtc aggctagaaa aaatgtttat
     2101 atagaaactc catattttat tccagatgaa agtctactaa aagctataaa gagtgctgct
     2161 cttagtggag ttgatgtgag aataatattt cctaaaattg cagaccacaa gatagtaaat
     2221 atagcatctt attcatattt tgaagaaata ttaagagcag gtggtaaagt ttatttatat
     2281 aataaaggat ttatacattc taaagtcgta ataatagatg ataaaattgc ttctgcaggt
     2341 acagctaata tggatttaag aagttttatg cttaactttg aggtaaatgc atttatctat
     2401 gatgaagaag ttattagggt aatgacagat gatttctttg aggatttaag tcactgtgaa
     2461 gaacttaatt tggaggtatt taagaataga aatatcatac aaaaaatcaa agaatctgta
     2521 gcaagattat tttcaccaat attataaaaa attattaaaa aaggtattga aaattatgtg
     2581 tgattggtat attataataa ttgttagcac tctattagat agagtgataa taaataaaaa
     2641 gtatatatat aaatctacat atataagtag tattaattta aaaataacta aaaacatagg
     2701 cgaactagtc tatacttaat attttaggag gcgtgaataa tgaaaataag accattagct
     2761 gacagagtag taattaaaaa agtagaagca gaagaaaaaa ctgcaagtgg aatagtttta
     2821 ccaggagcag ctaaagagca acctcaaata gctgaagttg tagaagttgg accaggtgga
     2881 atagttgaag ggaaagaaat aaaaatggaa ttgacagtag gagataaggt tatattccaa
     2941 aaatattctg gaacagaagt taagatagaa ggacaagaat acacaatact aagacagagt
     3001 gatgtattag ctgttattga ataaatatag aataaattta ttaggagggg tttaaaatgg
     3061 ctaaagaaat taaattttca gaggaaacaa gaagagcttt agaagctggt gtaaataaat
     3121 tagcagatac agtaaaagta acattaggac ctaaaggaag aaatgttata ttagataaaa
     3181 aatttggttc tccacttata actaatgatg gagtaactat agcaaaagag atagaactag
     3241 aagatagatt tgaaaatatg ggtgctcaat tagttaaaga agtagcaact aaaactaatg
     3301 atgtagctgg agatggtact acaactgcta cagttttagc acaagctata ataagagaag
     3361 gtttaaagaa tgtaacagca ggggctaacc caatactttt aagaaaagga atacaaaaag
     3421 cagtaacagt agcagtagaa gaattaaaaa atcaatcaag aatagtagaa acacaagagg
     3481 ctatatctca agttgcttct atatctgctg gtgatgaaga agttggaaaa ttaatagcag
     3541 aagctatgga aatagtaggt aaagatggag ttataactgt tgaagagtct caaactatga
     3601 atactgaatt agatgctgtt gaaggtatgc agtttgatag aggatttgtt tctgcatata
     3661 tggttacaga tgtagataaa atggaagcag ttttaaacga cccatatata ttaattactg
     3721 ataaaaaaat atctaacata caagaattat taccagttct tgaacaaata gttcaacaag
     3781 gtaaaaagtt attgataata gctgaagatg tagaaggtga agcattatct acattagtag
     3841 taaataaatt aagaggaaca tttgatgtag tagcagttaa agctccaggc tttggagata
     3901 gaagaaaaga aatgcttcaa gatatagcaa tacttacagg tgctcaagta atatcagaag
     3961 aattaggtta tgatttaaaa gaagctgatt tatctatgct aggtagagct tcatctgtta
     4021 aggtaactaa agaaagtact acaatagtag atggttctgg agataaaaaa gctatagaag
     4081 atagagtaac tcaaattaaa catcaagtag agcaaactac ttcagatttt gatagagaga
     4141 aattaatgga aagattagct aaacttgctg gaggagtagc tgttgtaaaa gttggagctg
     4201 ctacagaagt agagttaaaa gaaagaaagt taagaataga agatgctctt aatgcaacta
     4261 gagcagctgt agaagaagga atagtagctg gtggaggaac tgcttttgtt agtgttatac
     4321 cagcaatagg aacactaatt gagagtttag aaggagaagt taaactaggt gctcaaatag
     4381 ttaaaaaagc gttagaagag ccattaagac aaatagctat aaatgctggt cttgaaggtg
     4441 ctgtaattgt gcaaaatgtt gttaattctg aagcagaaac tggatttgac gcattgaatg
     4501 aaaaatatgt aaatatgata gaagctggta tagttgaccc aactaaagtt agtagaagtg
     4561 ctttacaaaa tgctgcatca atagcaagta ctttcttaac tactgaatct gctgttgctg
     4621 accttcctga aaaagaagat gcaggaatgc caggaatggg tggcggaatg cctggtatga
     4681 tgtaatttaa aaaatttaaa atataaattt taatataaag aactatttcg acttagattt
     4741 taaactctaa gttgaaatag ttcttttgta tatatctaat ttttgtatat ctataaattc
     4801 aatacacata tttagataat cagtatctag tttgtaataa tattttagag tagtattgtt
     4861 attttttagg ttatgatttt agatatatat taattaaggt ataattgttg atagagaata
     4921 aatcaaattt aaaaggatgc gataatatgg acttaagaaa attgcttgaa gaagttaaaa
     4981 atgaaaatat tgatatagat tttgcgttag aaaagttaaa agatttacct tatgaagacc
     5041 ttggatatgc aaatatagac catcatagag aattaagaaa tgggtatccg gaagttattt
     5101 attgtgaagg taagagtgat gagcatattt tagggattat tgataaaatg aatcaaaaaa
     5161 gttctaacat attggggaca aggtgcagaa aagaaacttt tctgaaaatt aaagaaatct
     5221 ataatcatgc tgagtacgaa gaggcgtcta aaattcttaa gattcaaaat catgatatag
     5281 agaatatagg aaaaggtaaa attgtgattg ctacaggggg aacttctgat ataccagtag
     5341 cagacgaagc ttatcataca gctaagtttt taggtaatga tgttgataga atatatgatg
     5401 ttggagtggc aggtatacat aggcttttaa ataaaagaca taaaatagat agtgctagag
     5461 taatagtagc cgttgcaggt atggaaggtg ctcttgcaag tgtaattggt ggactggttg
     5521 atgttccagt aatagctgtt ccaacttctg ttggatatgg agcaaatttt ggaggattag
     5581 cagcattgct agctatgtta aatagctgtg catctggaat atctgttgta aatattgata
     5641 atggatttgg agcaggatat ttagcatcaa tgataaataa actgtaaata cattgatata
     5701 aattaaacaa aactatataa taaaatttaa tttctattat atttttacta ttaaaattga
     5761 ggagacatat atgagtgaaa aaatacttta ttttgatata ataagcggta tatcagggga
     5821 tatgaccctt gcaagtttat taaatttagg tgtgcctaag gaaatttttt tggaggaaat
     5881 tcataaacta agaatgagcg atgaatttaa tataaacatc agctcgaaaa ctgaaaatgg
     5941 aatagtaggt acaaaagtag aagtaattac aaaagaaaaa catacacata gaaatttagt
     6001 ggatatattt gaggttatag ataaaagcac tctaaacgaa aatgtaaaat ctaaagcaaa
     6061 aaaaatattt atggtaattg gagaagcaga agcaaaagtt catggaacta caatagacaa
     6121 gatacatttt catgaggtag gggctataga ttcaattgta gatatagtag gagcttctat
     6181 attagttgac ttactttgtg tagataaagt ttgtgctagt acagtgtctg ttggttcagg
     6241 atttgtaaaa tgtgaacatg gaattatacc tatacctgca cctgcaacta tagaaatttt
     6301 aaaaggtgtg ccaattaaat taaataatgt aaatggagaa tgtacaactc caacaggagc
     6361 tgcaatcata aaagttttgt gtgatgagtt tgtagatgag cttgaatttt ctccaaagca
     6421 aattggatat ggtataggtc ataagaagtt tgaagtacca aatatactca gaactgtatt
     6481 aggtgaaaaa aaaaagaaga attaatttat gaaattagtg caaacattga tgatatgtct
     6541 tcagaaatct attcttattt gtatgaaaaa atattagatg agaaagcatt ggatatttat
     6601 acagagagca tttatatgaa gaagaataga ccagcaacaa aagtatcaat tttatgtagt
     6661 gaatcagatt tagaaaaatt tattaaaata ttacttttag aaactagtac ttttggagtg
     6721 agatataatg catataatag agaaattcta gagagaaaat ttgtaaaatt agatacaaaa
     6781 tatggagtgg ttacagtaaa actagggtat tataaaggaa aactagtcaa ggctactcca
     6841 gaatataatg aatgtaaaac tatagcaaaa aatgaagata ttcctataaa aaaagtgttt
     6901 gctgatataa attttagaat aaaagaagaa tttagtgaaa acttattgac ataagtaata
     6961 aattttgata aattaatatt cagccttatt aaataaaagt aaacagaaaa aaatatataa
     7021 aatataaact cactcgtata tgctcagaaa tatggtctga gagtctctac caagataccg
     7081 taaatatctt gactatgagt gaaattatta taccaaaaga cctagttaag taaatgtgta
     7141 ggctgagtta aactccctat gtgttggcta taagataaag gtattttgga agcctgtata
     7201 gtaatttcac ttttgctaga aattatggaa gttactatac aggttttttg tgtaaaaaga
     7261 tttaatataa tttagactgt attgtgttaa aataaatata taaaaactat attatacaag
     7321 gagagatgtg aatttatgaa acatgaatta gtacttgtaa tagattttgg aggacaatat
     7381 aatcagttga tagctagaag agttagagaa aacaacgtat attgtgaaat acttccatgt
     7441 actgcaagca tagaaagaat aaaagaaaaa aatcctaaag ggataatatt tacaggcggt
     7501 ccaaatagtg cgtatttaga agattctcca acaatatcaa aagaaatatt tgaattagga
     7561 gtaccaatac ttggaatatg ctatggtatt caaataatgt ctcatgtatt gggtggagtt
     7621 gtaagaaaag gtaataaaca agaaaaagaa tatggaaaaa cagctataac ttatggaaaa
     7681 tcatcattat ttgaaggaat aactacaaat agtgtatgga tgagtcatac agatttaata
     7741 gaaaaagttc cagaaggatt tactatagtt gcaaatacta atgattgtcc agtagcagct
     7801 atggaaaatg tagaacgaaa cctatatgga gttcaattcc atccagaggt ggaacactgt
     7861 ttggaagggg ataagatact tacaaacttc ttatataata tttgtaaagt aaaaggtgac
     7921 tggactacag attcattcat agaagataaa ataaaagaat taaaagaaaa aataggcgat
     7981 aaaaaagctt tatgtgcttt aagtggagga gttgattctt ctgttgctgc agttcttatt
     8041 cataaagcaa ttggggacaa cttaacttgt atatttgttg accatggttt acttagaaaa
     8101 aatgaaggaa atgatgtaga aagaatattt agagagaaat ttgatataaa tttaataaga
     8161 gttaatgcag aagatagatt cttatctaaa ttaaaaggag tttctgaacc agaagctaag
     8221 agaaaaataa taggtgaaga atttataaga gtatttgaag aagaatcaaa taaattagga
     8281 aaaatggatt tcttagtaca aggaacaatc tatccagatg ttatagaaag tggacatgga
     8341 aatgctgcta ctataaagtc tcatcataat gttggtggaa tcccagaaga tgtagacttc
     8401 caagaaattg tagaaccatt aagagagtta tttaaggatg aagttagaaa aataggatta
     8461 gagcttggta tagaagaagg attaatattt agacatcctt tcccaggacc aggtctagga
     8521 ataagagtta taggtgatgt aactaaagaa aaatgtgata tattaagaga agctgatgct
     8581 gtttatatgg acgagcttag aaaagcaggt ttatacagag aaatatggca agcttttgca
     8641 acattaccag atgttaagac tgttggagtt atgggtgatg aaagaacata tgcttattta
     8701 gttggtttaa gagcagttac ttcttctgat ggaatgactt ctgactggta taagatgcct
     8761 tatgatgtat tagaaagaat ttctaataga ataataaatg aagttgatgg agtaaataga
     8821 gtagtttatg atataacttc taaacctcct ggaactattg aatgggaata aaatatagaa
     8881 tgccaaaaat ccacataaat acaggattta aggtctctct tacttctatg ttatactggt
     8941 ttgatactat caatatttgc cagtatctta tggattaata agtaaaaaac atcctgctga
     9001 atgataaatt cagcggggtg tttttatttt gttttagaac tcctttgctc tgaaactcga
     9061 agtatttaac actgatgatt ttgttatctt ttagttcctt ttgtgtcatg ctctgaaaga
     9121 caaattgata caataaaaaa gtaatttctt aatcattgat ggagatatgt atttatgtta
     9181 ctcttgatgg taatttgagt aatattgttc atagcttttt atattcaatt aattagaaaa
     9241 ccatttggta taatcataaa taaacttgat gatataatag aaaagattta taattattag
     9301 aagtatataa aagtatttta tgtctatgaa atgtatgtct ataaaacata tagatgactt
     9361 ttgtatatta atgtatgaat attgatatta tagaagaatt gagttgtcaa tgatactaaa
     9421 tcaaatattt atagaagctg gagggattat gagaaataaa ataattatag gaattacttt
     9481 tttacttgta ttactaatgt ctggatgtgc ttcaaaacaa accgcagaaa taaacgtatt
     9541 agcaactact gatttacatg gagttatacc ttatgagtta acttcttatg taaaagaaga
     9601 aagaaaaaaa gataaaaatc taactttagt agatgcagga gatttttttg atagtggaga
     9661 agtgtttggt tctagcatgg atagttattt tagtgctaga tataaagatg gtgataataa
     9721 cactgaaagt tatattgaga cgcctatttc aaaagatatg aaagaggtag gttatgatgt
     9781 tgcagttctt gggaatcatg aatttatatc caacaataaa ttttatctag ataatatgat
     9841 atctgatttt gagaagcaaa aaataaatat tttatcggca aatacgtata aaaaaaatgg
     9901 agacagttat acaaaaccat acataataaa aaatattaat acacctgaag gaaacgtaaa
     9961 actaggaatt ttagggctta ctataaaaga agttggtgag aggaaacagt ggaaagatgg
    10021 aaaacttgtt gactctaagt ctttagaact aaaagaccaa gagggctaca atggtgaatt
    10081 gtatatgaat gatttagtag aagatgctaa aaagtgggtt agtataatgg aaaaagagaa
    10141 gcctgacatt atagttgctg tggcacatac aggtgagaaa cctaagaaac ctaaaaaccc
    10201 aggaaataga atacaagact tagctcaaca agttgatgga attgatgcta tagttgcagg
    10261 tcataatcat gttcaaattg agcaacatga ttataaaaat aaatcaggag aaaatgttat
    10321 agttactgag ccaggaaagc atggggaatg tatttctaag attaatttta aattagaaaa
    10381 aaacaatgat aaatggaata tagttgacaa atctagtaaa ttaattaaat ttgaaaaaaa
    10441 tcaagaaagt gataaatttg gagaatttat ttcttcaatt aataaagtaa gtaaagataa
    10501 gaaagaaata agattatcag aagtaaagac tcctgaatgg gataaaatat atgtttttaa
    10561 atctggaaca tctgcagatg atatatataa aacaatagga tataaatgga gaaatatatc
    10621 atctccaaag agtgatggca tggtgcagat tgttgtaatg aataataata agcctgttag
    10681 ctatatatat ggtgaagctg aagttatgcc aattaatata aaatgtgatg aatcagagta
    10741 taaagataat gttgtaacta tttatcctaa taaaaatgat aaatttaaaa ttgaaaaagg
    10801 taaacaagaa tatagtatta atttaaaaca tatacaatag aaacttttat cttatttaag
    10861 tttagattgt acttaaaata aagtgtgtat tattttaaaa attgatgata attaaaatca
    10921 caacattaag gaatagtaca cactttttat gctaaaaaat agaatgttaa aaaattaaac
    10981 ctttaaatta aagtacattg cttatattat ccaatattat cttcaaaggt tgtgtaatat
    11041 ttttatcctt atgtaatatt gctaacattt tattttccat atctatccca tctaattcaa
    11101 tcaatcgcaa ttttttatgt aagagtgaat ctaataataa attttctggc aaaatggtta
    11161 tacctaaccc tgcttgtgca gctttaataa gtgcaaaaga attaacactt tcccaaacag
    11221 gataagcttt ttggtttgaa agagataata cactgtccaa agtatcgcga atggcacttc
    11281 cttgctcacg taacaataac ggaaacgtgc ataactgtcg tagagataag gcttgtttag
    11341 tattggtggt ggttatgcaa tcataccttt aatccaagag aaagtagtaa atcaaactgg
    11401 atggataagt gagaaaatat ttacagatat tataactatt tcacaaatga caccagggcc
    11461 actggctgtt aatacttcta cttttgttgg acttcaaatt ggagggtttt ggggagcagt
    11521 tgcagccact ttaggttgtg tattatgtgg tgtagtaata tctctatttt tatataattt
    11581 ttttcaaaag catcagaact ctacttatat atttgaaata ttaaatggtc taaaatcagc
    11641 ttcattaggt ttaattatct cagctgcggc tacaattata attcttgcat tatttgggac
    11701 gaataaagtg tacttcaatt ctatatttga gatgctaaac tggactgcac taataatctt
    11761 ttttgttaca ctactgatag tacgtaagtg gaaaattaat cctattgtga ttataataat
    11821 gagtggtata ctgggaatat ttttttattt ataagttttt gctaagtaaa ggatatctat
    11881 ttttttatat cttaaatcca aatagccaat aaagtaaggc tatttggatt tttaataaat
    11941 tctgaaaaga taatcagaaa cctatataac atttaaatta tatcactggt tttctacaaa
    12001 ccaatcataa gcttcttggc aaaatttctt actatctata tagtaactat tatgaacatt
    12061 attaatttca acaagtttct ttttacattt tagtgaatta taatagctat caaaatattg
    12121 ggttttaaaa tatctatcat gaagtgtttt aaagacaaga gtaggtatgt tgactccatc
    12181 ttttatctta ctatcttttg caataaagca ttctaacatt gtattaatta tctttgtact
    12241 atttttccaa atatgaggat ttttcttttc aaatatttta taaaattcat tatcatagac
    12301 ttcattataa cctttatatt taggtggttt tactgttatg tgaaaattag gaagaaactt
    12361 aaagaaaaaa tataataatt tttatgttct gttttatata ttattcatat tttatcacag
    12421 ataatatact aaagttaatt tttttattgt acaactatta taataattct tctataatat
    12481 aagacattct ataattttat atttaagttc atgtgtattt aattgttttt atataaaatg
    12541 tccagtaata ctatttggat ttatttttat ttcatcagga gttccagaag caacaatttc
    12601 ccctccattt attccactgt ctggacctat atcgataatc caatcagaag cacgtataac
    12661 ctctgtattg tgttcaataa ttaaaagtga atgctgttca tttacaagtt ttctaaaagt
    12721 aattaacaaa ttttctatat ctttaaaatg aagtccagta gtaggctcat ccaatatgta
    12781 cagcattcgt tttttagttt ttgtccctaa ttcttttgct aattttatcc tttgagattc
    12841 tcctccactt aatcctaatg tattttgtcc aagctttaga tatccaagtc ccacttcatc
    12901 taataaggag agttttctat atatatcaga gcaatcgcta aaaaatgttt ttgcttctgc
    12961 tacttccatg tcaagtatat ctgcaattgt ctttcccttg tattgtatag acagtatttc
    13021 tttttgatat ctcttacctt gacattcact acaagttacc cattggtctg ccatatattg
    13081 cagaataatt tttgttttac ctaatccttg acagtttggg cattgcccct ttttgctatt
    13141 aaagctaaaa tagctttcat ccaataattt ttgttttgct tgtggagttt cagcaaatag
    13201 agaacgaatt tcatcaaaaa tattgatata tgtagcaggt gtagaacggc tagattttcc
    13261 aataggtgct tggtccatta agataaaatc atcaatagca tcaaatccag taaattcagt
    13321 ataattttta tctgggatag atttttgttt caatttttct tctagagcag gaagtaaact
    13381 gtgaaataca agagagctct taccagaacc actaacacct gtaatagagc acatacagtt
    13441 taagggaatt tctacatcaa tattttttaa gttatttgca tggcatcctt ttaagcttaa
    13501 ccatttactt ggtataattt tattttgtga acttatttta ttgtatgtac ttaaatattt
    13561 acctgttata gagtttggat tttttataat ttcttgggtt gtaccttctg cgataatgaa
    13621 cccccctttt gtaccagcac ttgggcctac atcaataata taatcagcag acagtattgt
    13681 atctttatcg tgttccacaa ctataacagt attaccctta tctcttagct cttctatcat
    13741 tttgatgatt aaattatggt ctcttgggtg taaaccaata gatggttcat ctaaaatgta
    13801 ggttaagcct actaattcac ttcctagctg acttgataat cgtactctct gcagttctcc
    13861 tccagataaa gttggagctg ttctatctgt tgatatatag ctaagaccta cctttaatag
    13921 ataactaact cgaattttta attctgttaa gatatcattt accaaagaga gctcattttt
    13981 ttgaaggtga tttggtaatt cacacaacca attccataac tgttctatcg tcatttttgt
    14041 tagttcaggg aagcgataac ctttgattgt agtgaatcta gcctcaatgc acagcaattc
    14101 gccaccacat gtattgcaag gaattttgtt catatattga tgcaaggtat tattttcaga
    14161 actagattct ctaaaaagtc tttgaatgtg gttgattgct cctgaagcag gtcttctaat
    14221 ctttgattct cgtccctttg attcaaagct aaattcatat atcttatcgt ctgtaccata
    14281 taagattgca tctataaaat ctcttggtaa ttcattccaa ggtatatcta ggtctatatt
    14341 tttatctttt gctattgcat ataattcacc taccatccaa ttaccatttg gctttttccc
    14401 cctcaatttt ccaaaatagt aagtagctcc atctaatatt gaaatagttg ggtcttttac
    14461 aattaattga ggattaactt gataggtaaa acctaatcca ttacaatcgt gacaagctcc
    14521 agtatgggag ttagcattga aggttgaagt atttatctta gaaaaaatag tttgacaatg
    14581 tggacaataa ttttttgtat tattcttttt ttctaatgga attttgcaat atggacagta
    14641 actttttccg attgatacaa ataacattct taaataatcc caaatgcttg taagagtacc
    14701 aacagtagag cgaggatttt ggcttccttt tttctgttcg atggctatag taggggttaa
    14761 tccctgaagt aaatcaaatt ccggtttttg aagctgttct gaaagttttg ctccgttact
    14821 tagattttca aaataacgtc tttttccttc ttcataaatc gtatcgaaag ctaaagaaga
    14881 ttttccacta ccacttacac ctgtaataac agttatttga ttttttggaa ttgtaacttc
    14941 aacatccttt aaattgtttt gccttcctcc ttttaaatag atagagcttt tattatgaca
    15001 aagtgaattt ttaattgatt tatttattgc ttttgtaaat ttgaatttct tttctagttc
    15061 aagaggattg cctaattctt ttatttccga aatacctaat atttttaact gagaaaagat
    15121 tgatttcaaa taatcttcag gcaatttttc tattaacaaa ttaagggctt ctatttttat
    15181 atttccgctt gataatatct ctgccatttc atctaataaa tttttttctg ctcttgttaa
    15241 ttgattgtat tttttggaac ttaattctgt ccataaatcc catggaagca attctaaacc
    15301 aatcatatta ttaaagtcat gtagaagatt accctttaca gaatgccaac ctttccaatt
    15361 tttattatat ccaaagtcat ctggatgagc atgtccttgc cgacatctta tccaggcttc
    15421 tgaaccagtt aaaaaaccat ctgtttttcc gacatcatga gtgtcaaaat taatttgaaa
    15481 ataatttttt tgtatgtcat ctatttgtgc atctattctt atccatctct ttgttagagt
    15541 atcatagtat tctacaagcc agtggtcttc ataagtaagc tcactttcaa aataattagc
    15601 aaaacctgct ctcattctag cctcaaaacc tcgatatctt aggagcgata caagaagtaa
    15661 agaataatct ctgcacattc caacaagcct ttgttttggg tttcttggaa tagttagtgg
    15721 gttagaattt atttcaaata ttctttttaa catttgagga attgtacgaa gcaattcctc
    15781 atcagattgt tgcttattaa aggatatacc atataatttt ccttggtctc catgaattac
    15841 taggctttga accactttta ataactcaat tggagtcttt ggtaaatctt gatatttatt
    15901 ttcaaatttt ttaggatttg tgatgatact ttgattttta tagttattta ttttcattta
    15961 tatttcacct ctctaaatgg atgatatacc ctgacatggt gtcatatgca aggacaattt
    16021 agatttttgg ccaaaattta aaaaataaat aaaaaatgat acagtgataa attgtaatac
    16081 aaatatagtt tcaaattgca tataatataa ttatttaata aaaccattat ctattgaatg
    16141 ttaatgaaat gttaagatgg cattcttgaa tattaagaaa tagaatacta taatatagtg
    16201 tgaaggattt ttagtaaaaa aatttgtaag tgttaatata cattaaaaaa taggtttata
    16261 atttggagga aaaaaatgaa attatcaagg tataagaaaa atagtttagt tctgtctata
    16321 attttatgtt cacttattat ttcaatcctt gtaattactg tttttaatac cgtaaaacta
    16381 cacaatatat ctaataaaca atctaaaaac tatttaaatg atgtatctac acagattgta
    16441 atgaatgttg attctaaaat caaatttatt ttatccgact taagaataat ggcagatttt
    16501 attaagcaat atgaaggtga tagtcgatat gagtatttat ctaaaagaaa gactagttat
    16561 aagtattatg atgttggaat aatagattta gaaggtaaag ctgagttttt aagtggaaaa
    16621 aagtttgact taaagaatac tgtttcctat aaaaaaggaa ttgaaggtaa agaatactca
    16681 gaagtaatag agtcaatggg ttttgttctt tatagtgtac ctatatttaa tgataatcat
    16741 gaagtatcta gtattttaat aggtgtatcc aataaagaaa gtatgaatga gattcttaat
    16801 atagataact ttaatggaaa aggaactatt gaaattatta atagtaaggg aaaacctctt
    16861 ttcataggaa aaaatactga attaataaag gaccttagcc ataaatataa gagttctgga
    16921 gatgaacctt gggcacaaaa gatgcttgag gattttaaaa atgataaatc agggaatatt
    16981 acaataacct catcaaaagg tgtacaatgc ttattaactt atcatccaat tacaagaggt
    17041 ttaaatgatt ggcattttct tctcatagta cctgaagatg ctgtacttgg tgaacttaat
    17101 aaattaaact cttttacaat aacaatgaca tttataatta catgtattat tggaattata
    17161 acattaattt tatatattat aagaagaaaa tatgtaaatc aaatagaaaa tattgcatat
    17221 ttagatagta taacaaatgg tataaattca actaagttta gtatgctagt caaaccttta
    17281 atttcagaat cacctgatag tacttatatg atgatagcaa tgaatataaa agattttaag
    17341 ctaataaatg attgtttcgg tagtgaaaag ggaaatatga cactaaaaca tctttacaat
    17401 attttgtata aaaacattga tgaaaatgaa gaatttgtgt gccgtcatga tgcagacctt
    17461 ttctatttgc ttattaagaa tagaccagtg ttagaagccc tggagttatt atataaaata
    17521 gaagctgaca caaattattt taataaaaat agggaaaatc catacttttt aagactatca
    17581 gtaggtattt acacaattga aaatcataat gaagatttaa ttacaataca agaccatgca
    17641 aatacagcaa gaaaaagctt taataaaact catcaaagtg atttttcttt ttatagtgat
    17701 atagaaagaa aacgattaat atctgaaaag gaaatttcaa atttaatgga aaaagccctt
    17761 gaaaataaag aattttttat gtgcttacaa cctaaaattg atataaaaag tggaaaaatt
    17821 agtggagcag agtctcttgt gcgttggaaa aattcagaaa agggcataat ttatccaagt
    17881 gattttattc ctcttttcga aaaaagtgga tttatatgta agttagattt atatatacta
    17941 gaagaaacat gcaaattgat ttctaagtgg ataaaagaag gtaaagaagt gttgagaatt
    18001 tctgttaatg tatcacgtca acatctgaat gataaattgt ttttagaaaa atataagcat
    18061 atatgcaata aatacaatgt accaacatgt ctgatagatt tagaacttac cgaatctatt
    18121 ttcttagaaa atccagaagc tatagatata atagaggata ttcattctaa tggatttaaa
    18181 tgttctatag acgattttgg atttgggtac tcatcattag gaatattaaa ggattttaaa
    18241 gttgacataa ttaagttaga ccgttcattc tttgttagca aaaataatat agaccgtggt
    18301 aaggtagtta ttaagtctat tattgaactt agcaaaaggc taggtatgaa agtaactgct
    18361 gaaggaattg aagaattaga acaagttgag tttctaaaaa atgtgggatg tgattatatt
    18421 caaggttatg ttttttcaaa accattatta atttctgatt ttgaaaattt tgcatatgaa
    18481 aataataaaa ttaaaaatat ccattattaa atgtacaaga taaaaaattt aggcaagtta
    18541 tataattaaa aatataactt gtctttttta tttatatatt taataaaaat atatttgcag
    18601 attttgtaaa aataattttc aaatgttgta agttgtggct aaagctttat tgtattattc
    18661 tgaatataga aagatagtta ctgtcttaga taaaaactga aacatacata ataatataaa
    18721 atttgctata ttaataactc taattatttt ggaaggaggg aaaatatgat aagtgggaga
    18781 atgttactca tcatgaattt tctaaaagga aaaaataaga caagttacaa ggatattagc
    18841 aaacaactta gtatagaaga aagaaaagtt agatatgata taaataattt aaatattgtt
    18901 ttgcgttcat taaacaagtc agtaatacaa aagatgaaca aaggtgtgtt aattattcct
    18961 gatgacttcc aagtagtatg tataaataat aacgaatatg tattttcctt aactgagcga
    19021 gtatctatta tgaaaattat atcaatgttt aaaatagata atctaaactt agaaaaatta
    19081 agtaaagaat ttcaggtttc tagaagttca attaaaaatg accttaatgt tttgtcaagt
    19141 gaattagaaa gaaaccaaat aactattact tatgataaat cttttaaaat aagtggagaa
    19201 gaagacatta tatataagca aagattgaac atattaaaat cgtattctta tctttttggt
    19261 aaggaaaaat ctgatttaag tgtatttgag aagtatttag tgaatgtaat tgaaaaaata
    19321 ttcaaaggga tttatttgat tgatatatat aattggtcta tgaatttatt attgcaaatg
    19381 ggatggactt taaatgatga atcttttaca tggtatgtgt caaacatttt cctttttaca
    19441 tggtatatac attcagatga aaaacatcct ttagaatctg cttcattaag tgagccgtgt
    19501 tttgaaaata agtttgacca agaatttgaa acaattattc aaaaaaagtt gactaaggaa
    19561 caaatttttc tgttaaagag ttttgtattt tttacaaata aatatgcaag tttaaacgag
    19621 gatatggatt tgattagtac agaaattata gttcaagatc taatttacaa tatgtcagaa
    19681 atattagaga ttagctttaa agaagatatg attctttata aaggattact caatcatatt
    19741 gccccattga tagagagaat caaacagaat atacagattt atgaggaatt acttcatgta
    19801 ataccagata aatatcaata tgtacttgaa gctactaagg tttctattca aaaaaacaga
    19861 gttttaagtc aaataaagaa tgaaaatgaa acaatacttt tggcaataca tttcttagga
    19921 agtatacata gaaatgagag gaataactac aaaaatatat tgttagtttg tggatttgga
    19981 tatggtgtaa ttgctatgtt aaaggatagt ttagtaaatg attaccagat aaatatagta
    20041 gagagtattc catcatataa gttacaagat tataaagaat gggaaagtat tgacttcatt
    20101 ataacaacat caaagatagt aatagacttg cctaaaacaa ttattgagat aaatccattt
    20161 ttacaagaag aggattattt gaagtttgaa gaaaaaggga taaaaagaaa gaacgtctta
    20221 actaattata tgtcaataaa aaaaaggttg gactttttag aagaagatgc ccaaaaaaga
    20281 gtattaagta ttatatgtca agaacttgga tacagtgatg agagaatatt atttagacaa
    20341 cttaatttaa gtgatttaat aggaattgat accataagga taattgatgg agatatgaaa
    20401 tgggaagatg ctgtaaaaat tagttcaaac atacttgcta attgtgattt tgtatcagca
    20461 caatatgctg agaatattat agatatttta aaaaatgtgg gtttttatgc tgtaaaagat
    20521 aacgaatttg ctttactaca tggcaatgat agttcattag tcaaagtaag ttcgataagt
    20581 ttattaatat gtaaagaagc agttagtttt ggagataaaa aggttaaaat catattttgc
    20641 ctagcaagca gagataaaaa agagcaaatt cctgcaataa ttaatctaac taaaatggtt
    20701 tataagacta attttattgc tatgttagaa agtgcaaaga caaaagaaga agcagaagtt
    20761 ttaattcata aatatgagaa ggaggtcact gtatgaataa attagttaat gtaaaatgtt
    20821 catttaaaat acatgttaat tcaaaagaag aagccattat atcgttagtt gatgtagtgt
    20881 caaaagaagg atatttaata gataaaaatc aatttttaaa agatgtttta aagagagaag
    20941 aaacattgtc gacttatatt ggacatggta taggtcttcc tcatagtcaa agtgtaggtg
    21001 ttaaaaactc ttgtattaca ataggaaagc ttgatactcc tattgaatgg acagaagaag
    21061 gagaaaaagt ggatttaata tttttgatat cagtaacaaa agacaatgaa aataatcttc
    21121 atttaaagat tttatcaaaa ttagcacgtt tattgatgca tgaaagtttt agaaatcaaa
    21181 ttagatgttc agatgaacaa actgtttata atcttataaa agaaaaaata gaggaggaag
    21241 attaaaatga gaattttaaa gtttttgcaa aaacatctta tgacagcaac atcttatatg
    21301 attccatttg tggttgcagg aggaatatta tttgctttat cagtaacttt aagtggacaa
    21361 gctgctgttc ctgaaacagg ctggttggct aaactgaatc aaattggagc tgctggcttg
    21421 gctttattca ttcctatatt aggtggatat atagcattta gtatggcaga taaaccagga
    21481 ttagctccag gtatgatagg tgcatattta gcaaaagaag taaatgcagg ttttataggt
    21541 ggtatcatag caggatttat agcaggattt gttgttttac aattaaagaa aattaaatta
    21601 gctcctacaa tgagaacttt aggttctata tttatttatc ctttagttgg tacattgatt
    21661 acaggtggta taattgtatt tttaattgga gaacctatag cttcttttat gacatggatg
    21721 acaaactggt taaatggaat gtctggtgtt tctaaaattc ctttaggtgg aatcttagga
    21781 ggaatgatag cctcagatat gggtggccct ataaataagg ttgctgctac ttttgcccaa
    21841 actcaagttg acacacttcc atatttaatg ggtggagttg gcgttgcaat ttgtatacct
    21901 ccaattggtt taggattagc tacattattg tttccaaaaa aatttagcaa ggaagaaaga
    21961 gattcaggta aagcttcact attaatggga tgtgttggaa ttacagaagg agctattcca
    22021 tttgcaacat cagatccagt tagagtaata ccttgtatta tggctgggtc gattgtagga
    22081 aatattatgg cttttttatt aggatgttta aatcatgcac cctggggagg tctgatagtg
    22141 ttacctgtag ttgacaatag gttggggtat attgcatcag tattaactgg agcagtagtt
    22201 gttgcggtat taatgaaatt agtaaaaaaa gacgtgaaag aagatgaaga aattgaagaa
    22261 gatttagatg aatcaattga attaatattt gaagaattat aaatataaat ggaaggaatt
    22321 tgtataaaaa agtaaatata aaactataaa tttaaaggag ataagaatat gaaaattgta
    22381 gcagtaacat cttgtcctag tggagtagca catacttaca tgtcggctga atcacttgaa
    22441 ttatcagcca aaaaatttgg tatagaaata aaggttgaga ctcaaggaag ctctggaata
    22501 gataatgaat tgtcacttaa agatattgat gaagcaactt gtgtaatatt gactaatgat
    22561 gttgagatta gaaatatgga acgttttaaa ggaaagaagg tagtaagaat gagtgtatct
    22621 gagataatta agaaatctga tgcattaatt aagaaaataa aagatacttt tcaataaaaa
    22681 ttgatttatt ttacatataa ctcaaagatg tttttaaaga aatttttatt aaaagcatct
    22741 ttgaaaatat cttaaaaata gaataaaagg agatatagtc atgaaaaagt taccaataaa
    22801 gactgtcgtg caaagattac taaacttaca agaagagggg aaaagtgcaa ctcttttagg
    22861 aattggtcct atgtcaccaa acttgttaca agcaagtttt gaactagcaa aagatgatga
    22921 ttttccttta atgtttatcg caagtagaaa tcaagtagat gcagatgaac taggtggcgg
    22981 ttatgtaaat ggttggaatc aggaaacttt tacaaaagat attaagaagg ttgccgacaa
    23041 agttggattt gatggtttat attatttatg tagagaccat ggtgggccat ggcaaagaga
    23101 taaagagaga aatgaccatt tgcctgttga tgaagcaatg gagttaggaa aaaaatctta
    23161 tttagcggac attgaagcag gttttgacct attgatgatt gaccctacaa aagacccatt
    23221 tgaaattgga aaagtaattc cactagatgt agtactagaa agaactgtag aattaattga
    23281 gtattgtgaa aacgagagaa aaagacttaa tctaccagac ataggttatg aggttggtac
    23341 agaggaaacc aatggaggtc tgacttcaac tgagacgtat gaaacattta taactagact
    23401 aaaggttgaa ctcgataata gaggtttgcc aatgccaaca tttatagttg gtcaaactgg
    23461 tactttaact agaaagacag aacaagtagg aacttttaat tttagaaatg cttatgattt
    23521 agctcaaatg gctaagaaat atggagttgg tttaaaagag cataatggcg attacttaga
    23581 tgatgtaact ttattagaac atataccatc tcaaattatt gctacaaatg tagctcctca
    23641 atatggtact gaagaaacta gggcatattt aaaattagca gaagttgagt gtaaattaga
    23701 aaaagagggc ttagttgaga aaacttctaa tatcagacat gttcttcttg ttaatgcaat
    23761 tgaatgtgga cgttggagaa aatgggtagt aggagaacaa aaaaatctaa ctactgaaga
    23821 gatatttaag gatgaagttt tatctaatga aatactagat attgcaggac attatacatt
    23881 taataatgat gatgttaaaa aagaaataga agtgctttat gataatttaa gtaagaataa
    23941 tattgatggt caaagatttg ttgtagacca tattaaacgt cctttgcgtg attatgcaga
    24001 atgctataac ttaaaaggtg ttactacgag gattttaaat aaataatagg acttatagtg
    24061 gtgagtattg ttatactagg tatttatagt tataattata cttaccacta ttaaatttag
    24121 gaggactaat attttgatta atttaatttt tgatgtagat gatactttat ataatcaatt
    24181 aacgcctttt tacactgcat ataataaagt gttttcttca ataaaggata tttctattga
    24241 agatttatat atgagtagta gaaagtatag tgatgaggta tttcatatga ctgaaaatgg
    24301 agaaatgcca atcaaagaaa tgcatattta taggataatg aaagcttttg aggagttggg
    24361 aaattctatt acagaaaaag atgcacaaag ttttcaagat gagtatatat atcaacaatc
    24421 acaaattaca ctaataccag aagttgagag gattttaaat ttttctaaag aaagaaatat
    24481 taatctagga attataacta atggcccatc aaaccatcaa agaatgaagt taaagcagtt
    24541 aaatattgaa aactgggttg ataaaagtaa tatttttatt tctagtgagg ttggattttc
    24601 taagccagat actaatattt ttagagtagc tgagaatgtt atgaatttag atagagaaaa
    24661 tacatattat gtgggtgatt cttatagaaa tgatgtatta ggtgctaaaa aagctggttg
    24721 gaagagtata tggttgaacc ataggggaca tgagttagaa gagttgtttt ataaacctga
    24781 ttttgtaatt ttggaacata aggatttgat atctttattt ataaaaatat gtagttataa
    24841 aaatatgtag taaaaaaatg ttaataatag aattgtataa aataattaat aataactata
    24901 taaaattata tgtttagatt gtgagcaatc tattttatgt taaaaataga ttatttagaa
    24961 gaaggggaaa tcttaaaaaa atttgaatat ccccttttta ttatttaatt aaaatgaaat
    25021 agttagaaat atattttatt tagtttagtc aataacattt tgggattctt tataactaca
    25081 tgttaactgt tgaataggtt tatcatatgg tttaacccat cctttattaa tacttaattc
    25141 tgtaagtgct gtgtgacctt ctaccatttg agttaatgaa gctgaatata tagctcttag
    25201 ttcaggtgta ctacttgtca atgcagaatt aagatacata ttggcagatg ctttagcaga
    25261 tgaaagcata cttaacatta ttactttatc gtctatataa gtattgttct taattagatt
    25321 ttctatcata tttctcatgt tatctatacc tcctcagaaa ttagtatttc attttcaact
    25381 ataaattgtt gtatagcttt aattcttcct tctgttgcta atatactaga ttctgattgt
    25441 ctttttagat cttcatctga tattaacata tttattgctc tttgcataag taatccgtca
    25501 gactccattt ttataattgc tgctaaagat attttttcag tttctgaaag tattctcatt
    25561 taatcatctc ctatattgta atttatgctt taatagtatg tctttctttt caaggaaata
    25621 accattacac ttttttacag tattttatat tctcgttata tcaaaattta gtcgacttaa
    25681 tatattaata taattttgta ggatatttat cattaaatat ctataaaata aattgtttga
    25741 atattcataa agcaaaattt atttgtatgt acttcttcaa gagtaaatat gagttttttc
    25801 aaaaaattaa tttttatgat aattattaag ggctatattt ttcttaaagt tgtgattatc
    25861 attattaatt tacaataaaa ttgccgaaaa gccctctcct ttaggtgtag ggatgaaagg
    25921 taaggtctta atataaaaaa gttagaataa tgcttgtaat ctaataccgc ta
//
LOCUS       DAMJYX010000046        25296 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJYX010000046 DAMJYX010000000
VERSION     DAMJYX010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.046"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            207..347
                     /locus_tag="PC479_002024"
     CDS             207..347
                     /locus_tag="PC479_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804567.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(344..715)
                     /locus_tag="PC479_002025"
     CDS             complement(344..715)
                     /locus_tag="PC479_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804568.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            complement(1270..2346)
                     /locus_tag="PC479_002026"
     CDS             complement(1270..2346)
                     /locus_tag="PC479_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2804569.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(2719..3234)
                     /locus_tag="PC479_002027"
     CDS             complement(2719..3234)
                     /locus_tag="PC479_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2804570.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(3597..4667)
                     /gene="leuB"
                     /locus_tag="PC479_002028"
     CDS             complement(3597..4667)
                     /gene="leuB"
                     /locus_tag="PC479_002028"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2804571.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(4674..5165)
                     /gene="leuD"
                     /locus_tag="PC479_002029"
     CDS             complement(4674..5165)
                     /gene="leuD"
                     /locus_tag="PC479_002029"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2804572.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(5180..6457)
                     /gene="leuC"
                     /locus_tag="PC479_002030"
     CDS             complement(5180..6457)
                     /gene="leuC"
                     /locus_tag="PC479_002030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2804573.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(6414..8156)
                     /gene="leuA"
                     /locus_tag="PC479_002031"
     CDS             complement(6414..8156)
                     /gene="leuA"
                     /locus_tag="PC479_002031"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2804574.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(8280..8526)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8674..9120)
                     /locus_tag="PC479_002032"
     CDS             complement(8674..9120)
                     /locus_tag="PC479_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804575.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(9141..9860)
                     /locus_tag="PC479_002033"
     CDS             complement(9141..9860)
                     /locus_tag="PC479_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2804576.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(9978..11252)
                     /locus_tag="PC479_002034"
     CDS             complement(9978..11252)
                     /locus_tag="PC479_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2804577.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(11452..12819)
                     /locus_tag="PC479_002035"
     CDS             complement(11452..12819)
                     /locus_tag="PC479_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2804578.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(13069..13533)
                     /locus_tag="PC479_002036"
     CDS             complement(13069..13533)
                     /locus_tag="PC479_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2804579.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(13538..14167)
                     /locus_tag="PC479_002037"
     CDS             complement(13538..14167)
                     /locus_tag="PC479_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2804580.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(14201..15088)
                     /locus_tag="PC479_002038"
     CDS             complement(14201..15088)
                     /locus_tag="PC479_002038"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2804581.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(15117..16355)
                     /locus_tag="PC479_002039"
     CDS             complement(15117..16355)
                     /locus_tag="PC479_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804582.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(16739..17881)
                     /locus_tag="PC479_002040"
     CDS             complement(16739..17881)
                     /locus_tag="PC479_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2804583.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(18022..18888)
                     /locus_tag="PC479_002041"
     CDS             complement(18022..18888)
                     /locus_tag="PC479_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2804584.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(19223..20770)
                     /locus_tag="PC479_002042"
     CDS             complement(19223..20770)
                     /locus_tag="PC479_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2804585.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(20763..21899)
                     /locus_tag="PC479_002043"
     CDS             complement(20763..21899)
                     /locus_tag="PC479_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804586.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(22162..23259)
                     /gene="dinB"
                     /locus_tag="PC479_002044"
     CDS             complement(22162..23259)
                     /gene="dinB"
                     /locus_tag="PC479_002044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2804587.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(23649..25154)
                     /locus_tag="PC479_002045"
     CDS             complement(23649..25154)
                     /locus_tag="PC479_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2804588.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
BASE COUNT         8358 a         4465 c         2670 g         9803 t
ORIGIN      
        1 tagatttttt tgttattatt acgagttttt tattgcaact gtagaataaa agtattatta
       61 tataattaag ataattatct agggaaattt tggataatca aaatatattt tataaaatca
      121 aaatacaatt acataaactt tattaaaaat ttgttgggaa tttctgacta acattaagaa
      181 cgtaagtttg ttgggggatg gtatatgtgg aaaagtcaaa cactactgtt acaaaaaaag
      241 aaaaaattga agatatagga atgaaattag aacatttatt gagtgaagat attacttttt
      301 atgaattatt acttgagatt ttgtataaag aaaaagaaga gaattaattc tcttctttaa
      361 ggaatctttt taaataggca ttatcaatca ttttttctat agcttctcta tcttcttttt
      421 caagagaata tattttttct aagagctttt ttgttttttc acttactgat aagttctcta
      481 tgattaattt atcttctttg cttatttttg tattatcatt tacgcctaat aaataatctg
      541 ttgatgtttc taatgctttt gctaatctaa ctattatttc acttttaggt tctcttaaat
      601 tattttcata acgtgacaag ctagcctcag ttataccgac tttagtagca agttcttttt
      661 gatttatatc taattctttt cgtagatttg ctattctatc tcctaaactt tccatatgta
      721 catcactcct taccgaaatt ataagttatt ttgataaaaa gtaaaataaa attaccaaaa
      781 atataaaaaa aaaaggttaa aataataaaa tatattgaaa attaccattt gtatatgtat
      841 aatatgaatt atacaaatgg taatttgatg agaggtggta tgttgaaata agaaactgaa
      901 ttaattaata aatacataga aagttttgaa aacctttaat tgagatataa acaatatgta
      961 caaaattttt tgcaagtaaa attaccaaat gtataaaata aatcagtagc atagataatt
     1021 ttatgtttta atacattaga ttatttaatt tagttataat ggtcaaaaat gcaatctaca
     1081 aataaaacaa agtattatca aaaaagtatt ctttaagaaa attttttatt agagatgtaa
     1141 ttgatataaa taatcgtatt aaaaaataaa atactaagtt taaaaattta aataatctat
     1201 aaatcaatta taaaatatta tttattatat attctaaatt tatcttaaaa catacatatt
     1261 tttacaatat tatttattaa aacctaatat atcatcaata ttatctaata attcttgtac
     1321 atcttcgaca gtatagtcac ccatatgtga aattctaaaa gttttttctt ttaaatctcc
     1381 atagccattt gctatttgga atcctcgttc ttgtaatttt gaatttaaat ttgatacact
     1441 aataccttga gtattttcta taacagtaag tgtattggat aagtgatttt cattggtaaa
     1501 aatttggaag tgttttttag cccagtttct aactaagttt gccatgtctt catgtctgtt
     1561 gaatctgtta tctaggcctt catctaatat attatctaat tggaaatcta aagcgaacat
     1621 gtgagaaaga gatggtgttg aagggtattg gtagtttttc ttgattaagt attcatacat
     1681 tgctaacaaa tcaagatata ctcctctaaa aggtacttgc ttagctctat cgattgcttt
     1741 ttgagaaaat gtgcaaatag acattcctgg tggtaatccc agtgcttttt gtactgatgt
     1801 tatacaaata tctattccaa tcttatcaac ctcgactttt attccaccgg ctgaacttac
     1861 agtatctaca cagtagatta catcctcata ctttttaaca acttctccaa tttcttctat
     1921 tggatttctt attccagttg aagtttcatt gtgagttaca gttattaaat cgtattctcc
     1981 ggtagaaagc accttatcaa ccatttctgg cgttgttgcc tctcctaatt caactttaaa
     2041 tatatccgct gggacattat tagccactgc cattttgtac catctatcac caaaggatcc
     2101 acaggaaaat actgctgcct ttttggatgt acaggaacga atggagcctt ccattaaacc
     2161 agttccggat gacgtggata aaagtatcgt gttgtttgta tagaatagtt tttgcatctt
     2221 ttcacttata ctcttttgga gcatagatgc atcttttcct ctatgtccta tcattggtgt
     2281 agccatcttt tgcaagactt cttctttaac atcaatagga ccaggaataa ataatttctt
     2341 actcattata attcccccct ttttagtttg taatatggat agcgttttta ctgtccttat
     2401 ttttaataac tataatatca agctgaatta actatgtcaa cataaaattt attttttata
     2461 tataatttat tttatatatt ttatgaaaac attcattttc taccttttca taagtctgaa
     2521 ttttctgaat tattccctca aaaaaaatat actttttatc tatataaatt aattttttta
     2581 gtatgtaaat tttacgctat tacgataaat ctttttagta taactaattt atttttatct
     2641 tgtattttta ttgtattttt gaattttatt ttttctaaat ataaaattta tgttttaaaa
     2701 tataaaattt ttatttaatc aaagttcaat atattccact ttttctttcg attcttcaaa
     2761 aaaccattct tcattagaaa aatcaccttc tgcaatttgt ccaataggca ttttcatata
     2821 ctttccagtt gctgtcactg ctatatctcc atttggtaga attatttctc ctgtaccttc
     2881 aaataatttt ctgttttctt tagttattct tccaacaact tttatagtag catctgttgg
     2941 tactggtttc ttatatttca actctaaaga tactgttact ccccatattt ggtcatcatt
     3001 tatacttaca gctcttccaa tagtctcatc caatattgca gcagacatac ctccatgaac
     3061 tctcccagga taactttgat gccaatcctt tgtattacaa attgatacaa gctctccatt
     3121 ttcaagttca taaaaatctg ctttaagtcc tagctcattt tgtgttccac aaactaaaca
     3181 agatttacta ctattttgct tcttcaaaac tttatacttt gccaaaaaat ccatctccta
     3241 accgacatta gtagtcgctt aatattaaat aattctaaaa tatattatat aatattaata
     3301 aaatccctgc aaacttctaa taataaaaat aggctacttc aatatgataa gtttaattta
     3361 aagtattatt ttccaaaaaa taactttatc aaaatagaaa ctcaaagtag atataaacct
     3421 ctttttcgtt attaaaatag taaaaatata tttgacttaa tataaaaaag agtgtctcat
     3481 taactaataa agtttatttt gagacacccc tcataaaaca aattatacta caatagattt
     3541 ttactaattc tacaagttat atatttaaat tatattattc aaattaaact ataattctaa
     3601 atttcattta ctattaattc gcccatttcc cttgttccta caacatttcc cacaccatta
     3661 tagatatcaa tagttctata acctttattg agtacacttt gtacagcatc ttcaatacac
     3721 ttagcttctt cttctaagtt aaaactatgt cttaacatca tgcttacaga tagaatagtc
     3781 gcaattggat ttacaatatc ttttccagct atatcaggtg cacttccatg tattggttca
     3841 tacataccaa aactatcttc tctaagtgat gctgatggta acatacctat agaacccgta
     3901 atcatagatg cttcatcaga tattatatct ccaaatatat tattagtcac tattacatca
     3961 aattggctag ggtcttttac aacttgcata gcagcattat caacatacat atgtgacagt
     4021 tctacatctt caaactcttt agctaagtct tccattatac ttctccaaag tcttgaactc
     4081 tcaagcacat ttgccttatc tacacttata agttttttat ttctatttct ggctatttca
     4141 aaagctctct taccaattct tcttatttca ttttcgttat atatttctac atcataagca
     4201 ttttcgatac catcaatgat ttttcttcca cgttctccaa aataaatgcc ccctgttaat
     4261 tcccttacaa caacaaaatc aatgcctttc tcaactatat ccgttctaag tggtgaagct
     4321 tcttttatac tttcatacat tgtagctggt cttaaatttg caaataaacc caatgactct
     4381 cttaatttta aaagaccaga ctctggtctt ttatctccct tacatttatc ccactttggt
     4441 cctccaacag caccaagcaa tacagcttcg ttttttcgac aagtttcaac agttttatta
     4501 ggtagaggtt ctccagtttc atcaatcgcg catcctccag ctaatacata ttcacaatcg
     4561 aatctatgat taaatttaca acaaatttta tttagaacct ttataccttc atctataatt
     4621 tccggtccaa caccatcacc ttttatgact gctatattac aattcatact tcactacccc
     4681 ctatttgctt ttattgagtt aattaagcct tcatttaata taatattttg cataaactca
     4741 ggaaatggtt ctgctttata ttcttttcct tttgttatat tttttatcac acctgtagaa
     4801 aaatctactt ctatattatc cccttcatct atgttattgg cagcttcttc acattcaagt
     4861 ataggaagcc ctatatttat agagtttcta aaaaatattc tagcaaaagt agaagcaatc
     4921 acacaggata ctccactttc ttttattaca attggtgcat gttctcttga tgaaccacaa
     4981 ccaaagtttt tagttgcaac tataatatct ccttttttta ccttagaaat aaatttatca
     5041 tcaatatcct ccatacaatg cgttgctagt tctttataat cagctatatt taaatatcta
     5101 gctggtatta ttacatctgt atctatattg tcaccgaatt taaaaacact tccattggca
     5161 atcataaatt ttacctccct taaattattt ctgatggttt tgatattttt ccagttatag
     5221 ctgatgctgc tgctacagct gggcttgcaa gatacacctc tgaatcaaca tgccccattc
     5281 ttcctacgaa attcctattt gttgttgaaa tggatttttc tcctgctgca agtataccca
     5341 tatgtccccc tagacatgga ccacaagttg gtgtagaaac tgctgctcct gcctctataa
     5401 aagtggtaat ataccctttt tctatagctt caagatatac tttttgagtt ccaggaaata
     5461 tcataactct aactccatct gctacttttt tgcctttcat tatctcagct actacttcaa
     5521 ggtctgatat tcttccattt gtacaagagc ctattacaac ttggtctatc tttatatcct
     5581 tatctatctc atctactgtt ctagtatttt caggaagatg aggaaaggct actgtttctt
     5641 ttagttcagc taaatctatt tcgtatactt catcatagac tgcatcttca tctgcttcat
     5701 atatatttac atcttttgtc atagttgaac acttatgtga atttatatat tccatagtct
     5761 tatcgtctac tggaaaaatt ccattttttg ctcctgcttc tattgccata ttacaaatag
     5821 taaatctatc atccatagat aagtattcta ctccatctcc acaaaattcc attgacttgt
     5881 aaagtgctcc atcaacacct atctccccta ttatatgaag tattatatct ttaccactta
     5941 tccatttatt tggtttattc ttcaaaacaa attttattgc tccaggaacc ttaaaccaac
     6001 attttcctgt agccatacct gctcccatat cagttgaacc tacaccagta gaaaatgctc
     6061 ctaatgctcc atatgtgcaa gtatgagagt cagcacctat aattacatct ccagcagtta
     6121 ctataccttt ttcaggaaga agaacatgct ctattcccat atctcctaca tcaaaaaaat
     6181 ttacaactcc ttttgatttt gcgaaacttc ttgtaaattt acattgttct gcactcttta
     6241 tatctttatt aggtgtaaag tggtctaaaa ccatcactac tttagtctta tcataaacat
     6301 cttcaattcc taatttttca aattctctaa tggctactgg tgatgttata tcattaccta
     6361 aaactacatc taaatctgct tcgattaatt gccctttctt aacttcattt aatttagcat
     6421 gtttggctaa tattttctgc gtcatagtca tccccatttt ttatttcctc ctcaatataa
     6481 cttttattta ttgcattcat aagtgcatta atagctgctg tatttatgtt ttccgatata
     6541 cttacaccaa aatagacttc attattgtgc tctattccta cataagctac ccctcttgaa
     6601 tgtgaccctt cctctaatgc atgttcccag taatatctaa atttataatc tttcatgcca
     6661 cattgcttta gtgcattatt aaatgaatct acaggtccat ttcctatacc aacaatcctt
     6721 tgttcatttc ccatataact tattcttgca gtcatctcaa tcatgttagt atcattagaa
     6781 tcatcatttt catagtttaa ttcatccatt gattttattt tatacttctt aactttataa
     6841 gggctctcaa cttcaatata ttctttctta aacaaattaa atattgcttc actactaagt
     6901 tcttccccaa ttctatcaga ttccattttg actacatttc caaaatcaga atgcatattc
     6961 ttaggtatca taaatccata atctgtttcc attatataaa cagctcctcc ttttccagat
     7021 tggctattta tccttataat ttctttgtat tctcttccaa tatcatgagg gtctattggt
     7081 aaatatggaa cttcccagta atcattatct ctgtttttca ttgctatcat gccttttcta
     7141 atagcatctt gatgtgaacc tgaaaaagca caatgtacaa gattacctgc atatggatgt
     7201 ctatcatgta ccataagttt tgtacaatct tcatatatac ctattatctt atctatattg
     7261 ctaaaatcta gctctgggtc tattccttgt gtataaagat taagtcccac atttactata
     7321 tccatattcc cagttctttc accatttcca aatagtgttc cttcaagtct atcggcacca
     7381 gccaataatc caagttcact tgcagccgta caagttcctc tatcattgtg agtatgcaaa
     7441 cttagtataa tagaatccct acaacttata tttttacaaa accattctat ttggtctgca
     7501 tatacgtttg gtgttgccat ttcaactgtt gatggaagat ttattattac ttttttttga
     7561 gtagttggtt tccaaacatc tatcactgcc tcacatattt caagtgcata atccatctct
     7621 gttcctgtaa aactctctgg tgaatattca aaagtaaatt ctgtttctgg atattttttt
     7681 acttcttcat taaataattt tgctccttca actgctatat ctattatttc ttgtttactc
     7741 atattaaaaa ccacatctct ttgaagaaca gatgtagagt tatataaatg tataattgct
     7801 tttttacatc cccttattga ttcaaatgtt ttttctataa gatgagccct agattgtgta
     7861 agtacttgta tagttacatc atctggaatc atattctcat ctattaattt tcttaaaaat
     7921 gtatactctg tatttgatgc tgatggaaat cctacctcta tttctttaaa tcctgtatca
     7981 ataagcattt tgaacattct gactttttca tttacactca taggtgttgg cagtgactga
     8041 tttccgtctc ttaaatcgac actacaccaa attggagcct taaatatttc attatctggc
     8101 catttacgat ttactatttg cattttatca tattttttat actttccaca cttcatgtta
     8161 tttccccctt tttctgtttt ttgtattcag tatacctcat atcgacgcaa tcctgcacaa
     8221 ttcataagga taaatgtcaa ttttatcatt agaataaaaa aaacctcatc tctaataaaa
     8281 ttagagacga ggttttgctc gcggtaccac tctatttagc tttctaaaaa gctcccttag
     8341 taatagccaa tacaataatt ataatgacta tatttcctat aacgtggaat tactccgatt
     8401 attctaagat ttaaatctta gaaaacagct cctagacaag ttcagaacta tcaactacta
     8461 aatcacacca tcctttagct ctctgaaagt ttcattgttc ttactacttc tattcatcac
     8521 ttttttaatc atttaaattt tctaaatatt ttgatttaat agttattata tacacattaa
     8581 aatgtattgt caacacaaaa aataattatc tcaatttttt atttttgata agcaattcta
     8641 taaaacatgc taactattga aaacagtatg atattatgcc ttaaactttt tcatcttttc
     8701 atctatgata ttcaataatt ttttttcatc ttccaaactc aatgccctta atctaatttt
     8761 ataattagat tcgtcagtgt atatatttaa tatatttttt actacttcta ttttttctat
     8821 tatcttataa ttaaactttt gactttgttt agattttata ctgaatatac attgattatt
     8881 tattaatatt gaatgattat cgagagtaat atatactttt ccctcatatt tatttgaaaa
     8941 atcacgacct tgtttaaata gaaaaactgc tgttattata cataaaatat ataatattgt
     9001 cgttgaatat ataaaaattg tagctaaaat tataagtgct aaaaccatat atatagtcat
     9061 tcctttttta aaataaccat tgtcataata attcatatag ataaacttat ttttactcaa
     9121 attttttcac ttccaagatt ctaaaaattt atatcaaaaa attctataat ttcatcttca
     9181 tgataattta tgactaattc atctggaaaa tctactgctt ctaaaacttt ttctgcattt
     9241 tcaaattctc ctatagaata acatatatga gagtcagtac caagaattac tcttgcacca
     9301 tatgttttac acaatgtcaa catatgactt acattttccc aagtacccgt tctatgagag
     9361 tttgaactta atgatgaatt atttatttct aaaaggatat ttttatcttt agcttttttt
     9421 actataggtt cataatctaa aggataccta ctgtcatctg gatgtccaat tatttttacc
     9481 ttaggtttat ccataacttt taaaatagca tttgtatttt cttcttttgt accagaagct
     9541 atacaaggtc tatgcaaact tgcgattata taatccaaat gttgcaacat atcttcttga
     9601 acatctaaat taccattata atccattata tttccttcta ttcctctaag aatctttacg
     9661 ccttgcactt cacgtggaag caaatgcaaa ttataaaaat agaatggatg tggtcctcct
     9721 ggcatattag gtccatggtc tgatattcct aagtatttta tattcttctt actcgcctct
     9781 tcgacatttt ccttgaaagt actatgtgca tgtccactta ttagagtgtg acaatgtaca
     9841 tccataatag actttttcat taaatttcct cctaatattt tacataaatt cgtgaaaata
     9901 gctaaagggc tatttcaaaa ctaaagctgt cataatagac agtgtaacgt tttaaaacag
     9961 ccccattttc aattatttta cacttctaaa tctttaaatt tatctttaaa taattctcca
    10021 atacttccta aagattcctc tacttctagg tattctgtta tatcctcttc tggctctcta
    10081 gttgcttctt ttattgaaac caacattctc ttagtctttg gtttaaaatc aagaatctta
    10141 actttaacct catctccaat attaacaaca ttagtaactt tagccactct agtatcactc
    10201 aattcagata ttggtaaata cgcatctaca ccttctttaa tttgaacaaa agcccctttt
    10261 tctattattc taagaacttt aacattgaca acatcatcaa tttgtacttc ttttgctatt
    10321 aaatcccaag ggtcattatt tatgtctttt aatgccaagg caattttttt agtctcttta
    10381 tttacatcca acacataaac ttctacttct tgtccctctg aaacaacatc ttcaacactt
    10441 tcaactcttg tccaagctaa attatttata tgtgctaatc cttcaatccc accaatatcc
    10501 acaaatgcac cataaggcat tatctttgtc accttaccat ctcttttttg tccaacttca
    10561 agagaattaa ataagtcttc tttagctgct ttgatacgag ctttttcttc ttctctttct
    10621 tgcttaattc ttgctctttc agcttctttt tctgccttaa tttgagctct tctttcttct
    10681 ctctctttat ttatacgctc ttgtaataca tctctatgag atgctacaag tctatttttt
    10741 ctaacatcca attcttttat ataaacttct aagttaagac caacaaattt agatgtatct
    10801 gtaataaatt ttgtatcaag ttgagaaatt ggtataaaaa atgtatatgt attgtaatta
    10861 gcaaatacac ctttatctat attcttttca acatttacag ttattattct atgttcatca
    10921 taagcctttt gtaactcggc aaaatcattt cttttgtcaa gttgtaattt agataaagtt
    10981 attgtaccat ctttttgaga tacttttgtt attacagcag ttatttcatc acctatgtgg
    11041 tatatatctt ctatatattg atttttttct atgttcaatt cacttatagg tattattcca
    11101 tcaaacccat agtctaatcc taattgtact tcatcatttc tcactgcagt tatttttcca
    11161 gttgtcaatt ctccaacttt cactttgctg aatacttgtt cttgctggtc aagtaattct
    11221 tgcattgtta aatcattttc cattataaac atcctttcct cttattgatt atcatatctg
    11281 taaatattat accattaata ttgcaaatac ttacattttt cttactataa tttttaaaat
    11341 aaattcaact tcatatattt atacattata gaaaatatct tattaaatta aaatacaaat
    11401 tctaattttt atctaataaa atattttcat aaaaaattta tattgtatat cttattcttt
    11461 tattgcttta ggttttgata gaaaacattt ataaaatata aagaaagaaa ttgctgttat
    11521 agtagcggct gttatatctg caactggctc tgctaaaatt actgcaaaaa gtttttcatt
    11581 aaagaatgtt ggaagtataa atattaaagg tattaaaagt acaatctttc taagtagagc
    11641 taaaaccaaa gatattgttg cttttccaag tgctaaaaat gtctgttgac aagctatttg
    11701 aatcccaaac ataaaagcac cagcaaaata tatttttata ctccaactag tcatactaag
    11761 taactgcgga ttcttattaa atatactaac aaaaaatgca ggaaatatca ttaataaaag
    11821 ccataaaata gtagaatatg taaaacatga caataaacac aacttaaatg tcttttttac
    11881 tctatcaagt tgacctgaac cataattata acttattatt ggttgtgctc cttgtgtaac
    11941 ccccatatta ggaagcataa gaatttgcat tatactgctc attatggcca ttgaacctac
    12001 tgctaaatct cctccataca tttgtaattt actatttaag gctataagta ctaaactttc
    12061 tgtagattgc attataaatg gagagattcc caagccaagc actggtaaga tgatacttgc
    12121 ttttggtatc atatacttct tttttatttt aagtatactt ttctttccaa agagaaacat
    12181 aagtacccaa atagcagaaa cgccttgtgc aattatagta gctaatgcag ctccttttac
    12241 acccatatta aagccaaata taaaaatcgg gtcaagtatt atgtttatta aagcacctat
    12301 agcaactgtc atcattccag ttgtagcaaa cccttgggta tttataaaag tattcatacc
    12361 cattgaaatc tgaacaaaca ctgtacctat taaataaata tttaaatagt ccattgaata
    12421 tccaattgta gattcacttg ctccaaacat ccaaagtaaa ggctcttttc caatcttaaa
    12481 acctatagtc aaaagtattg ctaaaatcac caatacagaa aaactgttac tcattatttt
    12541 ctctgcctca tcattgtttt gctcacccat ttttatagca gctaatggac ctcctcccat
    12601 accgattaaa taactacatg cagttattat aattattatt ggaaatgcca ctccaacacc
    12661 agccatagca acttctccat tttccattct accaataaaa atcctatcga ctatgttgta
    12721 taaaacattt acaatttgtg cgataatagc aggtgtagca agtttaaata ataatttacc
    12781 cacactttca ctacctaagt tagtgtttga attttccatt ttaaaacctc ctcaatccaa
    12841 taattaaaat gtatcactaa aattttattc tatataaaaa tttcatttta ttagcagaca
    12901 aatttagagt actttcccta caagtaatac tatactatat ttcttttgcc atagtaaaca
    12961 ttttagaact aactgaagtt aaatacttta tctcattttt tatttattat taaaaatttt
    13021 tcacatatac tcataaaaaa actatccaag tatattactt agatagtttt atattatatg
    13081 taaagaaaga gctgaaacta taacccctga taacaaattg ccaagaaata ctcccataaa
    13141 agcatctttt attctcattt ttaatatgga agctatagca gatgcagtcc atgttcctgt
    13201 agttggcagt ggtataccta caaatagtat tataccaatt atcgaaacac tttttaattt
    13261 cttcattctt gagtttattt ttctatcaat tacatcagca atccctttaa atagtttctt
    13321 ccctcttaaa aattgtaata catgtctgaa tgtcaatatc aagggaatcg ataccaatgt
    13381 agaacctatt acactaaata cataaaccca tataggattt aagcccattg caatacctat
    13441 aggaatggct cccctcaatt ctgttacagg aaccatagat aaaattaata tgtgtaaata
    13501 tcccaaacta ttccctccta ttctaagtga cattaagtta tgaatttatt tgctctataa
    13561 cctcaccatc ctttttacta actaaaaatg catttttagt caatgaaagc attggtaaat
    13621 cactcttaga gtctgaatat gcatatgaat tttcataatc aatctctatc ccaagtttac
    13681 ctaatacagc ctttattctt tttacttttt caattccctt acagttttct ccaatgaatt
    13741 gatttttata tcttgaattt tcataaaaaa actccgtgcc tataacctcg tctgcaagac
    13801 cataatactt aaaatatttc atatatgcat atggagatgc agttatcata attataacat
    13861 tatcttcttc tttatttttg taaataacct ttttcataga ttcgttaatt tttttcatta
    13921 tgcaatcatc aaaaaacttt tttaaatctt cttcactaaa atctactatt ggataaaaga
    13981 ttgcctcttt tgccttcttc aagggtatca ttctaattaa atacataaat agaataaaaa
    14041 taatgtatgg caactttata atagttttaa aggttttatt ttttaaagag taaataaaaa
    14101 aatctatcat gctatcagta tttataacag tataatctac atcaagtaat aatacacttt
    14161 ttttcataag tatttcccca tcctatcact aaaaatatca ctaaaaaatc aatattatta
    14221 aacatgtaat tatccataag attacgttta tttttaaagc attatctttt aggacaatat
    14281 cagtaggatt attttcattt gtagtgttaa ttatataatt atatctaagt acaccataaa
    14341 ctacaaatat tgttgtaaat atcatatgtg gcttttctgg attacttgta gaatacaatg
    14401 cataacatac aattgtagaa cttaatataa ctatcatcat ttgatttaga ttttctatgt
    14461 cataatcttc caatattttt ctatgttcaa ctgcatcatc tcttagagtt tctatttctt
    14521 ttttacgttt tccaagagct aagtacagag ataagaaaaa tgtacataaa attatccaat
    14581 ttgataaact aacacttatg gctacacatc ctgcataaac cctaagtata aaaccaagag
    14641 atattgaaaa cacatctaat agtattatat tctttagttt aagagaatat aatatattgt
    14701 ttatcaaata gagactgaga attatagaaa gattaaaact aacaactgtt gataaacaga
    14761 ctgaaattaa cgctaatact acaaatagta tattagcact ttttttgctt attcttccac
    14821 ttgccactgg tctattacat tttttaggat gttttttatc tttatccatg tctatagcat
    14881 cattcagtgc atatactgat gatgacatta ggcaaaaaca cacaaacgca ataaacgtat
    14941 ttaaaaataa atttatattt agtatgttgt ttgaaaaaat tatagctgaa aatacaaaga
    15001 aattctttat atattgcttt attctcatta actttatata atctgaaatt ttagtaagaa
    15061 gccttatact tgtattggtc tgtttcataa acttactacc ctttcaatat tattttttaa
    15121 acaggatatg attttatccc tatgtttaaa atttctataa atccattgac attactgatt
    15181 actatagtaa ccaataacag cgatagaaat atattataca tattcttttc attttttcta
    15241 agggctttcg taaagtaagc caataaaatt ataactgcaa aactaaaatt tatagccata
    15301 atacttgaat tgactagatt atatcctact ataccatgta aagttagatt gaatataatt
    15361 attcccatac atagcttagg aactcttttt tctgaatttt caataactga taaaacacaa
    15421 aaacataaag ctaataatgt aagaattact ggtattgtag caacttctcc attttgcaaa
    15481 aataaccctt ttttaatact tgctccagga aaataaaaaa ccgaatacaa taatctctct
    15541 ataatcattg caatcttttc caaaattggt tctccactta taaatttcgt atcatactgt
    15601 attacatttc caaggttcat aaaactctca taagacttcg ttgaaactac acaaatatat
    15661 gccattagga taaatgtaat taaatgtttt aacatttttc ctatttttaa tttaaactca
    15721 aatatcatat taaaaaaata caaatatata ttactaatag ttattccaaa tagaggaata
    15781 gctgctaatt ctaccaaata agaatttttt cctttcatct tatttgcaat aactatgaaa
    15841 aatattaatg aaaattggct atatataaat ctctctacat ttaaagtcat aaataactgt
    15901 ggaaaagaaa atatcattat catagtaagt aaaattaaag ttaatctctg catttttatc
    15961 tttttaatat gattaaagac taaagttatt gacatagcac tagttattat ttgtagacaa
    16021 attagtccaa acccataggc actattaaaa tctgttgtcc caggatttgc tattgaagta
    16081 caaaataggt atattggata tgcaaaaatt gatataattg ctgaaaacaa tatatgctta
    16141 ctattgtctt gacttatagc aaatacattt aaattgaata aaactcctgt atctgtatct
    16201 aacaatacat cataagtatt gtttaaatca taaaatatag tagtcttaga aaaaaatatc
    16261 ttagttatta ttaagtaagt tattacaact aatataaaca aaaacatctc gtttttatat
    16321 tttaatattt tactcatcaa tatatcacca tccattcatt atataggatt tgctgacacc
    16381 tacaatttat tccttatatt taatagccat cttcataatg ttcttacttg ttttattgta
    16441 attgaataac tctttattta caaccacaat atttacttta ttgatgttat aatcagtcaa
    16501 gtattgttta tataacatag attagtgttg tataaaatga gaatttgtaa gcaatattaa
    16561 tgtaaaatat ttgaaaaact ttatataaaa atatacaatg ttacttatgg aattaaacta
    16621 taacaaatta aagcataatt taaggagaca aaattataat ctgtctcctt aaatattaaa
    16681 actatatttt aaaacattac taaatactat acaaatttca aatatagtat aaaattaatt
    16741 agactttaac actattagct ccatcttctt cccatttttt aatttctttt aatctatcta
    16801 ttataggtaa atgctgagta cattcaactt cacattttcc acaagatata cattctttag
    16861 cttttgcctt actattcatt gtgtaattcc aatgtcctaa gccataaact aactttgtca
    16921 tttcttcatc actcttttta aacatctgct tttcattata aaaaagcata taggctgggg
    16981 tattaatacc ttttggacat actttacaat aaccacaacc tgtacatatt tcgttcatat
    17041 tcttatttaa tctagtctca atatcatgta tatcctcatc tgaataaatt ctattttcgt
    17101 ctgcaatttt acatgcatca tctatatctt gttttttatt aaaccctatc aaagatatac
    17161 ttatttgtga aattcctatg ttgaatctta aagctgattc tatagtactt tcgccatctt
    17221 ttgatagaaa agaaagttct ttattatact ttggaatagt tccaccactt aatggattca
    17281 ttgccacaac accatatcca ttatcaacag catatttggc accttttagc ctatatggaa
    17341 agtttaatat atttattccc atagttacac cttcaaactt attttcactt agaatatcta
    17401 taacctcatc tcctggttga tgagatgaaa atactatatg gtctatcaat ccttcttctt
    17461 tacatttcaa taatccttca tattgacctc caggtctcat tgctagttcg taatgttcca
    17521 tctttctaat attccaaaca tgataaaaat ttattttaga tacgcctaat cgttctattg
    17581 atttttttac agcctctata gctttttctg atgtatcaca tgcttttggc ttttctttag
    17641 ttgatacata aaaatcggtc ttaccttcct ttatcatttg tctaaaggct agtccaaata
    17701 tatcttcact tctgtcatca caataccctg gtgctgtatc aaagtaatta ataccttttt
    17761 catacgcata tttgactaaa tcagcatttt cttgattact tttttctaaa tcaaacctta
    17821 atcctccata gccaactact gaaaccattt tccctgtctt tccatattca acgtatctca
    17881 ttgtaaattc ctccttagat atattaagac ttaatttctt tatagaaaat attataccat
    17941 aaataatttt tataaattat ttatggtata atattttttt tataaattat atttttttat
    18001 aacttgtatt ttatagctaa gttaaaaact ataaaaaggt tcaatatgga ctattacttg
    18061 agcattttca ttaatttctt cttgtatttg tttctcaatt ttatgtgtta aatcatgaga
    18121 ttgctctacg cttataaatg ggtctaccat tacatgcatg tcaatataaa tagaactttt
    18181 gctacctcta cttctgatat tgtgaacatc ccttacctca ttaaattcaa acacaatttc
    18241 ttttatataa tcctcatcta cagccactct atcgaccaaa attccaatag atggcctaaa
    18301 tattccataa gccgaataaa ttatgaatgc agaaataata ataggaacta tagattctat
    18361 tattactgga aatcctaact taacaccaac taaagtaacc aatacaccta gagacacaaa
    18421 tatatcactt cttgtatgca atgaatccga aatcaaaaca taactattta gtttagtccc
    18481 aattctatat tcatacatgc atacaaaaat atttataaaa agtgtaataa ttaaagcaat
    18541 taaactttct atagttatag caggtacaac tggattagct attcttaaca ctgcactaag
    18601 aattatctta attgctataa caaacaacat accacttata aataaactgg ttataatttc
    18661 aaacttttta tgtccataag gatgattttt atcttttggt ttagatgcaa agaaaatacc
    18721 tataagcccc actatattag atgctccatc tgacaaagaa tgaaatccgt ctgctgtcat
    18781 actatagctt tttatttgat taccaataat gatttttaat aatgctactg caaaattagc
    18841 aaataaaatt atccacaata cttgcttcac tttcttatag ttatccataa caaattcccc
    18901 ctcccttcga ttcaataaag aaagctatct atcaatatca atcaaccaga caataaaatc
    18961 gtatataagc acatttacta tatttatatt caaaattggt tgtacttgtt cattctattt
    19021 gaaacttaat tataaataaa aatcgtaggt atggttttgg tatacataaa aataagagac
    19081 tgtctcaaaa taaagataat cttctttttt attgttgaaa tgataaaata tatttgattt
    19141 catataaaaa agagtgcctc atgaactaaa aagttcattt tgagacactc tcttaaattt
    19201 tttgatataa actattaatt ttttatacta agccttcttt aactaagaat gaatgagcaa
    19261 cttcttctgg tgatttacca agttcatcca cttgatagtt aagttctctc atagtttctt
    19321 catttatttt tcctgaaagc atattcataa ctttttctaa ctcaggatat ttcttcaaag
    19381 tatcttctct tactaatggt gctgcataat atggtacaaa gaaattttta tcgtcctcta
    19441 atatcttaag attaaacttt ttaataagac catcagttaa aaatgcatct ataacctgag
    19501 attcattatt atctaaagct gtatatctta aacttccatt cattgctttt acatctttga
    19561 attttaaacc ataatctcta gaaagcccta caagaccatc ttctctattt tcaaactcca
    19621 aagttggtga gaatgtaaat tctttaccat atttagtcaa atcagaaata gtatttattc
    19681 catacttttc agcagtttca ggcatcatag ctaatgtgta agtattatta aatccaagag
    19741 ggtcaagtaa agttaactga ttttcttttt ccatagtatc ttttacaaca ttatatgctt
    19801 catcagcatc tttaatcgga gcatgtttca ttacatttac caaaagtgta cctgtatagt
    19861 caacatacat gtctaactca ccagatttaa ttgcattaaa cgcaacagat gaaccaccta
    19921 gatttaaatt cttcttaact ttaagattcg tatttttttc tactaattca gcataaacat
    19981 taccaagtat aagttgttct gtataatctt tagaaccaat agttattgta tcttttttac
    20041 ttgaaaaact agaactaaaa acaacgaaca acattgaaac aacaaccact acagatatta
    20101 ctttaagtgc tatataattc ttcttaggtg cttttggatt taatcctttt ggagtaactg
    20161 caacttcaat tttactaaat aaataatcaa ctaacagtgc aattatacat gatgggattg
    20221 cccctgctaa aatcatatta ttatttacag tttgaacccc tgaaaatact aaatatccta
    20281 aaccaccagc acctataaat gctgcaagag tcatcaaacc aacagctgta actgcagata
    20341 ttctaactcc acccattatt ataggaagtg ctaaagggaa ttgtatttta aataatgtct
    20401 gatttcttgt aagaccaata cctttagctg attctaatac tactgggtca atacttgtta
    20461 tacctatata cgtatttttt acaattggta gtaatgaata aaccactacc ataaatatgg
    20521 ctggtttact tcctatacca agtataggta caagaagacc caaaagtgcc atagacggaa
    20581 ctgcttggac taagtttaca aatccaagta caaactttct aagagatgag attcttgaaa
    20641 taattattcc aagaggaaca ccaactatta tagctattaa aatagatgta acagttagac
    20701 tcatatgttg tattaaaagc tctataatct tatctttttg taatagtata aagttaaaaa
    20761 aattagccaa gaacactcac ctccatttct aagaattgtt cacttaaaac agaaagtaaa
    20821 ctacttctag taataagccc taccagttta ttttcatcag aaataaccgg aatatatcca
    20881 accgaattac gattcattac atttagtatt tctactaaat tatccccttc atttacatgc
    20941 aaaggctcac ttgacataat atcagcaagt aatattgact tatcatcaat atctttcata
    21001 tcttttacag taacaatacc ttttaatgtt tttgctctat ctataattag taagctatca
    21061 accttactcg ttctcataat ttctattcct tgagttacag ttctagtaga attaacagca
    21121 ataggatttt ttatcataat atctcttgct tttatatatt ctggattatc ccaaactcta
    21181 tcttctccga taaaatcttt tacaaagtca tttgcaggct ttctaagtat attttcggga
    21241 gtatcatatt gtgctattct tccatccttc ataatacaaa ttttatcagc tatctttaaa
    21301 gcctcatcca tatcatgcgt tacaaatatt atagtttttt tcaattcatc ttgaagagaa
    21361 aataattgtt cttgtaaaga tgttctcgtt attgggtcta aagcactaaa aggctcatcc
    21421 atcaatatta tttcagcatc agtagcaatg gctcttgcaa caccgattct ttgttgctgt
    21481 ccaccactaa gttctgaagg atacttattc aaaaattcat cactatcaag tcctaccatc
    21541 tcaagtagtt gcaacgttct tttttcaatt tcttcaacgg atttttcctt ttttaggcgt
    21601 ggaattagct ctatattttc ttttattgta agatgaggga ataggcctgt gttctgtata
    21661 acatatccta tttttcttct aagctctatt tcattttcct gagaaattgg tttgccatta
    21721 atatatatct caccagatgt aggttttatt aatttgttta taagcttcaa agtagtagtc
    21781 tttccacaac cacttgaacc aatcaaaact accaatgtac ctgtttctac aactagagaa
    21841 atatcatcta atataacatt atttcctatt ttttttgtta catttcttat ctctatcata
    21901 atttatcatc ccctttttcg aacaagcaac cttctccaat attataagtt gtttttataa
    21961 ttatatcata ctattttatt tatgtaaaca aagtacgatt tttaggtcac tatttgtctg
    22021 aatattgcta caaatataaa gtatttatat atagtatata atatacaatg tcaaaaaaca
    22081 aaaaatataa aaatattcat aaaataacac tagcctcaat tatatagtat taaagctagt
    22141 gttatttcat ttaaacaaaa ctcacatttg gatactatcc atgtattcaa ataatgtaat
    22201 atctttataa gtttcttttt tatcacttag gtttgatatt gtaactccca atagcctaat
    22261 ttgttttcca ttaacctcaa catttctcaa caaattatct acaccacttc ttatatcatt
    22321 atattcgtca atatagtgtt ctaaactaag gcttctagtt atctttgtaa agtcctcata
    22381 tttaattttt aatgttaatg tttttccaaa cttctcagag ttttttaatc tatgacatac
    22441 ttcttcacaa agttcatcaa gaatgttgcg tgtttcttct tcatcaatat ccaaattatg
    22501 acttaatgta gtctcagccc caacagattt tctaacccta tttggttcca ctggtctatc
    22561 gtctattcct ctagcaaatt tatataattc ataacctctt tttttgaata tattttcaag
    22621 ttcaaataaa tttaaacacc ttaaatcata tccagttttt atacctaaat ttttaagagt
    22681 atttgaagta acttttccaa caccaaaaaa tttattcaca ggtaatttat ctaaaaagtc
    22741 ctgggcattt tcttcggtta ttacagttaa cccatttggc tttcttaagt ctgatgcaat
    22801 cttagcaaga aacttattgt atgacacacc agctgaagaa gtaagaccaa cttctctaaa
    22861 tatatctttt tttatttgct ttgctatctc aattgatgaa tcaatatttt ttttattttt
    22921 tgttacatct aagtaagctt catctaaaga taatggttca actaaatctg tgtatctata
    22981 aaatatatct cttatttgct ctgacacact cttataaaca tcaaatctag gtcttacaaa
    23041 aactgcatat ggacacctat tgtaagctac agctgatggc atagctgaat ggataccaaa
    23101 ttttctagct tcataagaac aggttgcaac cacacctcgg ctttgtggat ttccaccgac
    23161 aataacaggt cttcctttta attttttatt atctctttgt tctatagatg cataaaaagc
    23221 gtccatatct atatgaatta tttttctata atgtttcatt attatcacct tttaaagtat
    23281 aaatagatta attttattta tattactttt taattatacc aaacttatgt ttctttttgg
    23341 tattttttca ttattttctt tatatattca tattttccat aaataaaacc atctataatc
    23401 cattaacttc aatataattt ataaaaaatc cttaaaaaca cttgcaaaaa ctatataaaa
    23461 gctttacaat aggtattcca aggaactaaa tttaaaattc cttatggata acagtatata
    23521 tatttaattt atgggatgtt ttaaaaacaa aagactgtct tatacaagac agcctactga
    23581 ttttattata attgtaatgg actgctcaaa ctattacttt aaaacagccc atattttttt
    23641 atataatttt aactatagta aagattttgt gaaggatatt ctgggtcaca agttacatct
    23701 ataccaagtt ttctaaatgt ttgttcatca tctctattta atatagtagt agaatgagct
    23761 tgacaccctt ttaataatgc taacttttcc atagcaaatt gtgcagttgg attagttaca
    23821 gcacaaatac taagtgcagt taacacctct tggcaactaa gtgaaacatt tctactagct
    23881 aaagtctttg tttttaagtt tatgataggt tctaatatta caggtgatat taagtgcata
    23941 tcatcagata tattggctaa atgtttaatg gcatttaata ctactgctgc agttccattc
    24001 attaaatcag aacccttacc agttaatatt gtaccatctt ctaattctaa tgcaactaca
    24061 ggacaagtat cattcttgtc ggcactttct ttcaatttat tactgtagtt tctagcaggt
    24121 aatacaacat ttctatcttc tggctttaag ttaagctctt ccataatcat tttcattcta
    24181 tcataagcac ctttatcaac ttgtcccttt ttatactcac aggcagtttt aaagtatctt
    24241 cttattattt cttctataga agcttttctt attacttcat catctattat tccaaagcca
    24301 actctattaa cacccatatc agttggagat tggaaaattg attcttttcc agttatcttt
    24361 tctattattt ttttaagcac tggaaataat tccaaatctc tattgtagtt tactgacatt
    24421 tcaccatacg tttcaaggtg gaatgaatct atcatattca catcttttaa atcaactgta
    24481 gctgcttcat aagctatatt caaaggatgt tttacaggta cattccaaac tgggaatgtc
    24541 tcaaacttag aatatccaac tacattacct cttttatttt catgatacaa ctgacttaga
    24601 caagtaccaa gttttccact attaggtcct ggtgctgtga ctactactat tggctttgta
    24661 gtttctatat attcatttgc tccatatccc tcatcactaa ctatagtctc aacatctgac
    24721 ggatatccct ttgttggata atgcttataa actttaattc ctctacgttc taatttattt
    24781 ataaatacag ttgtagcagg ttgtccttca aatctagtaa ttactacact atttacatct
    24841 agttcataac ctcttaaatc atctattaat cttaaaactt ccatatcata agtgatacca
    24901 aagtctcctc ttattttgtt tctttctata tctcctgcat agacacaaat tacaacttca
    24961 actttttctt tcatatgttg taatactttt atctttgcat tttcatcaaa tcctggaagt
    25021 actctttttg catgaaggtc tcccatcaat tttcctccaa actctaaata aagtttgtca
    25081 aaattattta ctctttctaa gatatattta gactgctctt ctaaatattt tttgtggtca
    25141 aatcctactt tcatcttaac acctcactaa attaaatttc ttgttgataa cagtatattt
    25201 atatatattt ttttagtaaa acatcttata aaaaaatgtt aatacttttt cactcaaagt
    25261 aatattatac aattaagatt aatttttttc aagata
//
LOCUS       DAMJYX010000047        25023 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJYX010000047 DAMJYX010000000
VERSION     DAMJYX010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.047"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            151..534
                     /locus_tag="PC479_002046"
     CDS             151..534
                     /locus_tag="PC479_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2804589.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            536..886
                     /locus_tag="PC479_002047"
     CDS             536..886
                     /locus_tag="PC479_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804590.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(987..1445)
                     /locus_tag="PC479_002048"
     CDS             complement(987..1445)
                     /locus_tag="PC479_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804591.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1490..2278)
                     /locus_tag="PC479_002049"
     CDS             complement(1490..2278)
                     /locus_tag="PC479_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2804592.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2417..2854)
                     /locus_tag="PC479_002050"
     CDS             complement(2417..2854)
                     /locus_tag="PC479_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2804593.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3676..4185
                     /locus_tag="PC479_002051"
     CDS             3676..4185
                     /locus_tag="PC479_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804594.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4591..5127
                     /locus_tag="PC479_002052"
     CDS             4591..5127
                     /locus_tag="PC479_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2804595.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(5554..6354)
                     /locus_tag="PC479_002053"
     CDS             complement(5554..6354)
                     /locus_tag="PC479_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804596.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6549..8147)
                     /locus_tag="PC479_002054"
     CDS             complement(6549..8147)
                     /locus_tag="PC479_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2804597.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8475..8651
                     /locus_tag="PC479_002055"
     CDS             8475..8651
                     /locus_tag="PC479_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804598.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            8825..9955
                     /locus_tag="PC479_002056"
     CDS             8825..9955
                     /locus_tag="PC479_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2804599.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            10196..10546
                     /locus_tag="PC479_002057"
     CDS             10196..10546
                     /locus_tag="PC479_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2804600.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10554..11003
                     /locus_tag="PC479_002058"
     CDS             10554..11003
                     /locus_tag="PC479_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2804601.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11105..11680
                     /locus_tag="PC479_002059"
     CDS             11105..11680
                     /locus_tag="PC479_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2804602.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            11673..13082
                     /locus_tag="PC479_002060"
     CDS             11673..13082
                     /locus_tag="PC479_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2804603.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            13266..14699
                     /gene="eutA"
                     /locus_tag="PC479_002061"
     CDS             13266..14699
                     /gene="eutA"
                     /locus_tag="PC479_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2804604.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            14717..16081
                     /locus_tag="PC479_002062"
     CDS             14717..16081
                     /locus_tag="PC479_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2804605.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16094..16975
                     /gene="eutC"
                     /locus_tag="PC479_002063"
     CDS             16094..16975
                     /gene="eutC"
                     /locus_tag="PC479_002063"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2804606.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            16995..17648
                     /gene="eutL"
                     /locus_tag="PC479_002064"
     CDS             16995..17648
                     /gene="eutL"
                     /locus_tag="PC479_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2804607.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            17659..18360
                     /locus_tag="PC479_002065"
     CDS             17659..18360
                     /locus_tag="PC479_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2804608.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18363..19832
                     /locus_tag="PC479_002066"
     CDS             18363..19832
                     /locus_tag="PC479_002066"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2804609.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            19928..20215
                     /gene="eutM"
                     /locus_tag="PC479_002067"
     CDS             19928..20215
                     /gene="eutM"
                     /locus_tag="PC479_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF2804610.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20342..21103
                     /locus_tag="PC479_002068"
     CDS             20342..21103
                     /locus_tag="PC479_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2804611.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21116..21745
                     /locus_tag="PC479_002069"
     CDS             21116..21745
                     /locus_tag="PC479_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2804612.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            21787..22512
                     /locus_tag="PC479_002070"
     CDS             21787..22512
                     /locus_tag="PC479_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2804613.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            22525..22797
                     /locus_tag="PC479_002071"
     CDS             22525..22797
                     /locus_tag="PC479_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2804614.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            22790..23341
                     /locus_tag="PC479_002072"
     CDS             22790..23341
                     /locus_tag="PC479_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2804615.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23353..24444
                     /gene="eutH"
                     /locus_tag="PC479_002073"
     CDS             23353..24444
                     /gene="eutH"
                     /locus_tag="PC479_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2804616.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            24446..24919
                     /locus_tag="PC479_002074"
     CDS             24446..24919
                     /locus_tag="PC479_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2804617.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
BASE COUNT         9829 a         3140 c         4173 g         7881 t
ORIGIN      
        1 gaaaatcctt ataaaaattt aaattgtaat gaataggaag taattaaaaa gaatttttat
       61 tgatttttaa aaatttagtt agaaactata tcattatggt aaaaaatgta ttataatgtt
      121 tagaaatatt ataataagga gcgattaaac atgaatctat cgaagaaaat agaagataaa
      181 agaaaagcta aacagagagc tgaaaaagtt aagaaagcta aaattgctac agctggtgtg
      241 gtattaggag ctgttactgg tgcagtgagt ggagtacttt tggctcctaa atctggaaaa
      301 gaaacaagag aggatataaa agatgcttca caacaaattg ctgaaaagat aaatatgaaa
      361 actgtagatg taaaaggcaa agtatctgag aagctagagg ataaaaaagg aaactttata
      421 gaatcaaaga aaaaaataaa aaaatatata gataacaaaa agagtgttga aaattctagt
      481 gaggaagata aaataataga tatagttgaa ccagttagtg ttactgagga ataatatgga
      541 caatacaata acgattaaca taagtataat atatgtatta ttagctatac ttgcttgtat
      601 tgctttagtt tatttaatta ttgtacttgt taaactacat aagattttaa ataatgttgg
      661 aactatgtta aatgaaaata ctcaaaatat aaatacaact ttaaataatc ttcctaatgt
      721 tgtagaaact tttggagaag taggagaaaa tatgaaagat gtaacagaag tagttactga
      781 agttacagca gattttcttg ttactaaaga cagtgtcaaa tcaaatatgg atgttatagt
      841 tgatatattg actataatta aaaacatttt tctagataaa aaataataag tttaattaaa
      901 ataagcctat aagatataat ttaaacgacc ttaaaatagc atttataagg tcgtttttta
      961 ttagcttgat ttgacactat gtgtttttaa taattggtaa tattcctctt tatcatttga
     1021 acatatatat ctatgcttgt tgtttcaatt acatccataa gaacatcttt acattcaagc
     1081 tcaaaatctt ccattccttc taaatattca tcatcttcat caaaaaagat ataagaatta
     1141 tatagccatt cttcaaattt ttcttcaagt atatcccaat acgaactcca aaataatcca
     1201 caacctgata tataagatgc tctactttga tttatataaa cctcctctag aagcttttgt
     1261 aatctactgc tataaagaaa atctgctctt ccactgcttg atattaattc atttaaatct
     1321 tctattgaca ttaattcaac tttaccactt ttatcataag gagtataatt ttcatcaacc
     1381 catttatgtg gtttgttttc tctgcatact gttgctatca actctacaac ttttgttctt
     1441 atcatttttt tatcctaact tatttatttt ttataaaata atcatgattt tataatttat
     1501 ctatttcttg taatgctaat ttaacagttt catatctatc agcaccattt attactacat
     1561 caccttttac aagtttatta gcttctccac ctacaacata aaccttttta cctaaacctt
     1621 gcttataact agacgcttca attattaaag aatctttcaa ttgccaatgt aaaatctgag
     1681 cagccacttt atcagcatca cctgtataaa cgattacatt ttcatacact ttattttccc
     1741 ctccctcttg agaatttgat atagatttgt ttagtatacc ttctgctatc aatttagcaa
     1801 ctttatcttt attattaaca taataatctg tatctgcttt actatctaca aaacatactt
     1861 ctattaatat tgctggtgca atagtatgat taagccaata aagacctcta acatcagact
     1921 ttgcgcccct atctttgaat actgtagcta acctagtatt cactctttct gcataggttt
     1981 tgccattatt agtcttatat attgtttctg tccctactgg agttgaagtt gtggcatttg
     2041 aattaaaatg aatctgaaca gccaaatcag tttcctgttt atttgctata gcacattggt
     2101 ctgctaaatg attacttgat tcatcaactc ttcctgtata aacagtagct ccacctattt
     2161 taagccattt aacaattaag tcattcagta ttcttgtttc tttgctttcg tttatatacc
     2221 caacagctcc tgtacctttt ccaattaaag tgtgccctgg tattattact actttcaaac
     2281 taacatctcc tttacaatta aatttaactt ctatttaata tatgcataac ctagaatttt
     2341 gtttcttttt atatcttata cttttattat aacccatttt gtaaaaatat agaatattaa
     2401 gtaaataatg ttaaaattat aattttgtat ataagacatt acttgattgc aataatttta
     2461 tagcatattc atctattcta tatccagttc tataatatat atgtttaatt ccagcttgaa
     2521 ttatagcctt agtacaattt aagcaaggaa agtgagttac ataaatagag caatttttaa
     2581 ctgaaatacc ttcttttgca caataataaa gggcattttg ttcagcgtga atggttctaa
     2641 tacaatgtcc atctctcatt tcatgaccaa cttcactaca atgcttatct ccagaaatac
     2701 taccattata tccagtagac actattctgt tttctgaatt gacaattata gctcctacat
     2761 aagctctatc acaagttcct ctttcagcta ctgtttcgca cagtctcata aaatattctt
     2821 gccatgagca tctattttca agttttttat tcaaattata tacctccaaa acttatatct
     2881 aatatataag atttatacct taaaaataat attaaaaaca aatttatacg ataaataaaa
     2941 tttattaata ctgtttttag tctaaattat ttcattttaa attaaagtta caaggaatct
     3001 aaaattatat atatctttct tcgtaatatt aatgttacaa tataaaaaac tgtctcaata
     3061 aaatagagac agttttacag tcaaagttta taatacaagt tcatattttt ctttaaattc
     3121 ttttacagct tcgggccatg ccttttgttc tgtaatacct ttttctttag ctattttaat
     3181 tgcaatctct cttatcattg cagcgccttc taatattccc atttttaatc tcccttcaaa
     3241 tatctttttg tatagaatat aaatagtgct aagatttatt acatgctgca tatacaatat
     3301 ctatacaaca cataacttgt aaattccttt tattttcata cgtctttaac cgacatataa
     3361 ttatattcaa taagttttaa aattatgaac tcaattataa ttatttttat ttttttagaa
     3421 tctaaacact aaaaattgta ggtaaatttg tcttatgcct ttataaatat ttctacagtt
     3481 ttaattactg gacatatcta tttttattat aaaaagcgat taaaaatcga gaatatccgc
     3541 ataaaaactg tatataagca aaacggttaa aggaaaaaga agaaatttac ttgaatgtta
     3601 aaatgcgttg cttgaagcaa tgggcagttt tgcctataat atcggtaaca actgaaagaa
     3661 aggtggttag ccctaatgtt aagcgaaaac attaaaacaa tcaggaagtc aaaaggactt
     3721 tcacaacaag aacttgctat aaaactgaat gtagtgcgac aaacaatatc taaatgggaa
     3781 caaggactgt cagttcccga ttctgatatg ttgatttcca tatcagaggt gcttgaaacg
     3841 cccgtaagta ctttacttgg agaaactatt gttgagtcgg aagttgataa cttaaaagtg
     3901 atttctgaaa aattagagat tataaacttg caacttgcac aaagaaaaaa catgagaaga
     3961 aaaatgtttc attggttatt tattttatta tgtgcagtca tattaatagt ttttgtggtc
     4021 ttagggggac taaatagtcc ttacttggat tggaattata ccgaccctga aaccgctgtt
     4081 tttggagtag cttttcatgc ttttgagtgg ctatttgtca gattagtacc gattgtcctt
     4141 ataggagcaa ttattggaat ctttttgaca cgtaagaaaa tatgaagtaa ctctgctttt
     4201 tataatacag caagcgttcc ttaaaagcgt cattctacat ggcaatcgag gacgactatg
     4261 taaggaagaa cctttttgat ttccaactaa gcgaagttat tgaggacgac agtaagacca
     4321 aaaccgcatt aagtaaagta caggaagcaa gactgctgga cttatactct catgcgtcag
     4381 atgcaggaaa tgcgaggtct ggtagcatga tttacaacga tttgtacaac gactgctagt
     4441 agaaataata tggagataag tattatctac aaagtcagaa aagaaatgac ttgataattt
     4501 actcatgtta taattattga tgtgaaatta ataaatcgaa attccttttt tggatttaat
     4561 aatactgagt aaggaaaaag tgaattactt ttgcaaggag gtgttaatat caggcaagta
     4621 aagtcttttg tttttccagt tatatcctta atattcttag accaaattag caaagttctt
     4681 ataggattat tcttaatgga ctttgaaatt gatataattg ggaaattttt aagattcaat
     4741 cctgttcaaa atacaaatct ctcttatggg ggaaacttta ttggtattct atctaattta
     4801 tgggtattgg tattgtttaa cattttagtt atattagtta ttatatctgg atatgctttt
     4861 tataaatcaa aaaatgaaca aacaagctat tcagtaaaag taattatgtc ttgtggactt
     4921 gctggtacaa tatgtagctt gatagataaa ttattttggg gaggaagttt agattttttg
     4981 cagataccaa gcttttttat tttcgactta aaagactgct accttactgt tgcagaaata
     5041 atatttgttg tcataggaat tttgcataat agagaaatat caatgaaaga atacatatat
     5101 ttttgctatc gtcagtttaa aaaataaatt tagaagataa atttaagttt caaggtaaac
     5161 ttatttaatc acagcaaata gttggcttac ttaagctttt gggctaaact gtgagcacta
     5221 attaagtctc tgatatctaa caaacggagc acaaaaataa aaattaaata taaagcacag
     5281 ttaattttga ttaaaaagtt gactgtcttt tcttttgtag aaatttaagc ataggtaata
     5341 aagaaatgac tgcatttaag cattatgtat ataattggag gcaataaaaa gccagcaaag
     5401 cccataaaat agagctgtgc tagcaacatg atgaattata agaagataat caaaaactta
     5461 cgctttattt taatttaaaa aaatgattac tgtaaagaaa tcatttttta ttataatcac
     5521 tgatacatca aatattctaa ataaaatttt tatttatctg gaattgtata ttttttctat
     5581 aatattaact acagcgtaca ttgcccaagc acagatagcc aatacaaata ctcccatcat
     5641 tactaaatct aatttaaata cttgagaacc atatactatc aagtagccta ttccatatct
     5701 tgaaactaaa aattctccaa ctattacacc tacccatgcc ataccaatat taattttagt
     5761 taaatttatt aaatttcctg tgtttgcagg taatatgagt ttaaataata tttgagattt
     5821 agtagctcca aaacttttta acatcttaat tttttcttca tctatattca taaaataatt
     5881 ataagctgaa agtatagtaa caacaacaga aatcgttaca gctgttacga cgattccttc
     5941 tattccagca cctacccaaa ctataattat aggagctaaa gcagtcttag gaagagcatt
     6001 taaaactacc aaaaatgggt ctaatatttt tgataatttt tctgaccacc aaagagcaat
     6061 tgctaccaaa atacctaata cagtacctat aacaagacca agaacagttt catatacaga
     6121 tattccaata tgtttaaata attgaccact aaatgcatat tgaataaata aattgtaaat
     6181 atcacttggt ttgctaaata gaaatgtatt tataacattt aagtcagcta aaagttccca
     6241 gattacaata aaaccaatta aaattaatag ctggtagaat aatacatttc ttttttctct
     6301 ttttacacct ttaacatagt ttatatatcc ttcagatctt ttattaagcc tcatgtgtat
     6361 ctagctcctt ccataatatg tcgaaatact tttgaaaatc aggaacttta cgtgttttga
     6421 gaggcgtttt tgttccatcc atgttaagtt caatatcgta aatatttttt atggtagctg
     6481 gtcttttaga tagtattgca accttatctg atatgcttat tgcttctgct atagctaaaa
     6541 aagtgcaact aattgcttaa agaaacagaa ttatcaactt cagcacccat ataaacaata
     6601 ttaagatttc ctaaactgct atcccaaacg atactattaa taatagtaga taataacaat
     6661 ctcttatcat ctaaactggc attatcaact tcatcattaa atctcttcaa attactaaca
     6721 ataatatcaa tattaatact gtcactacta atattgatgt tactagattc caaagtatta
     6781 atcttatcct ttaaaatact aatctcatta tttaggctct caatctggga aactatataa
     6841 tcagaagcag tactaccagt attttttgca agctctataa ctaaattttt aataagtctt
     6901 tccttatcta aaatttgatt tttaagtaaa ttagtctcag atttaaaaat attaaaaaca
     6961 ttatcagtct taatgttatt actattgaat gcacttataa tagactcttc tttacaattc
     7021 ttaattttat ctataactaa actttcaact aatggaccat taagattttt acagttgcaa
     7081 ctatcagacc caagtgtttt tttagtacca caaatataat aatactttaa atccttgtca
     7141 gtttttttgc ctttataggt aattctcata ttagaaccac atttaccaca ttttataaga
     7201 ccagataaaa gagcagtttt tccagttgat atccttggag ttttatcacg atttttatct
     7261 aattgatttt gtacttcaag ccatatttca ggttctatga ctccatggtg ttttgcaatt
     7321 gctgctattg agttagatgt atttttagca taggtaagga ttccacattt tctatctgga
     7381 gtacctacaa catccatacc actattttta aggtaatcta aaatttcatc attagctttt
     7441 acatatgctg gatttctaag tattctagat aaagaactag ggtcaaattt gccaccacga
     7501 ggacccctta ttccattatt atagacatat ttatataact tagataagga cctaagttct
     7561 aaatatttat tgtatattag ctttacaatc tccatttgtt catcatttac ttgaagctta
     7621 tacatacttc tttcttttag attttcatct aagaatgtga ttttttcact gttgaatcca
     7681 tgaggtgtat tgcctccaag ccatctacca ctacgggcta attcatacat attgtctttt
     7741 atacgttctg ctattgtttc tctttctaat tgagcaaata cacttgatat aaacatcata
     7801 gctcttccca ttggagttga agtatcaaat tgttctttga tagatataaa tgatatgctt
     7861 agttcattta atttttctat taaagtagag aagtcagata catttctcga tatcctatct
     7921 aatctataac aaactaggta actaaatttc ttatctttgg catcctgcat cattttctta
     7981 aattgtggtc tatctatatt gctaccacta aatccctcat cttcatatat caaaaaatct
     8041 tttacaccaa tagactttgc ataatcaata cataattgta tttgattttc tatagaatca
     8101 ccttttccag taaatcttga ttttcttgaa taaattgcag ctttcaaaat taaaaccccc
     8161 tataagttag gatatatatt tgaaactatt tatctacaac cttttaagta aacttattag
     8221 aatgaattta ttaattaggt aaatttatat aattctactt tttataatat atgactattt
     8281 attttatttc tttactattc aagttctaaa tcaataataa attttgatat ttaattgtat
     8341 caggacaaat ctctaatttc atagattatt aatataaaat cataggttag tgtgaaaaag
     8401 ttgaaattta aatattttaa aagataattt ttttgaacta ataggtataa caaaaattta
     8461 tagggggatt gcatatggaa atcaatgtta tagaaatttt ccctaaagat aaagctaaac
     8521 ttaataaaat agaaatggat aaagctagtt ggtttgtaaa tataataagt aaaaaatatc
     8581 ctaaagaagc tttaaatgaa gcatttagta ctttagaaaa agaattaaat ataagtaaag
     8641 ctaatacata agctttgatt attgatttta tatactattg gttttatacg ctatgacttg
     8701 acattatatt aatcaaatag tatattttta aatatagcac aatgaagtgt taaaagagca
     8761 atggagctcc atagataaag tctatggagc ttttttgcgt atttaactta aggaggatag
     8821 gactttgaat aactttgaga ttaaaactag aataaaattt ggagaaggct cactaaaagc
     8881 tttgcaagaa ttaaaaaaca aaaatgtact aataataaca gaccctttta tggtgaaatc
     8941 aaaaacaata gataaaataa catctaattt aataaactca acatatcaag tttttgaaga
     9001 aatagtacca gacccaccaa tagaagtagt agtagcagga atagaagcat ttaaatctat
     9061 aaatccagac gtaataatag cattaggtgg tggttctgca atagatgcag caaaatctat
     9121 aatggacttt tctaaaaaaa tattaaaaat taacgaagta gagtttatag caataccaac
     9181 aacaagtgga acaggttcag aagtaacatc atttgctgta ataacagata gtcaaaaaaa
     9241 atgtaagtat ccattagtga gtgatgattt acttccagat atggcaatac tagacccaga
     9301 acttgtaaaa actgtaccaa attttataac agcagataca gggatggatg tattgacaca
     9361 tgctatagaa gcttacgtat ctacaaattc taatgacttt tcagatgcac tagctgaaaa
     9421 agctataaag ttagtattta aatacttaat aaaagcatat aaagatggaa atgacataga
     9481 agctagagaa aaaatgcaca atgcatcttg tttagcaggt atggcattta accaagcatc
     9541 acttggcata aatcattcta ttgcacatgt attaggtgga aaattccata taccacatgg
     9601 aagaacaaac tctatattac ttcctcatgt aatagaatat aattcatcaa tagtaggata
     9661 tactaacaca acttactctg ctacagctaa aaaatatgca caaatagcag aattagtagg
     9721 acttgggagt gctaatataa gaatatctgt tagaaattta gtaaatgaaa taaagaaact
     9781 acaaaaagaa atgaatatgc caactaaatt gacacaatgc aaattaagtt tagaagatat
     9841 aactaatgaa gaaagagaaa tagcaaaact tgctataaaa gatacttgta cattaactaa
     9901 tcctaaggta ccaactgaag atgatataat aaacatatta aagaacatta aataactaca
     9961 ttttattaca tgtaaatata atattataca aatagggaaa atgtttttaa aaatataaat
    10021 tatatattga caaatataaa acataaaact ataatgaaag tatcacaaag gcgtgatact
    10081 ttagtagaaa gcaatggagc ttcttaggaa tattaatatt attcttatgg agctcctttt
    10141 attttaatca aatagctttt taataaacta tattcaaaga aagcagggaa taaatttgac
    10201 tgaagaatct aagcaaagag ttattcaaga atatgttcct ggaaaacagg ttacattagc
    10261 acatatcata gctaacccta atgaagatat atataagaag ttaggattag tacttgataa
    10321 aaaagatgca ataggaatac ttacaataac accaagtgaa gcgtcaataa tagcagcaga
    10381 tgttgctaca aaggcttcaa atgtatcttt aggttttata gatagattta gtggttcagt
    10441 agtaatatca ggtgatgtaa gttctgtaga atcagcgtta aatgatgttt tagaagtctt
    10501 aggaaatatg ttaaacttct catctacaaa aatcacaagg acataataat acaatgaaga
    10561 atataatatt tatgggaaag acaggtagtg gtaaaactac cttatgtcaa aaactagacc
    10621 agttagaatt aaagtataaa aaaactcaat cagtggaact ttataataat gcaatagaca
    10681 ctcctggtga gtatatggaa aatagaaacc tatataatgc cttaataaca acatcagcag
    10741 atgctaaaat tatagcaata gtgtatgatg ccacacaaga agaaaattat atagcaccag
    10801 gttttgccag tatgttttgc aaagacgtaa taggaattat aactaaaata aataaagcta
    10861 ctgaaaacga agtcaatata gcttatgaaa gactatcact agcaggagca agtcaaatct
    10921 ttaaaataga cacagttgat aatattggac ttgaaaaatt atttaaatat ctagaaaaat
    10981 ataaaaatat agatgaagtt taaggggaaa tattttttta ttcaaaaata ttattaaagt
    11041 atgataaaat aaaaaaatgt ataaactatt ttaagaagat ttaatcttta agcgaggtag
    11101 gaacatgggg aaaaaaatac tattggttga tgatgaacca ctgttaagga tggatacaaa
    11161 agatatatta gaaagtcatg gctatgaaat aataggcgaa gcaagtgatg gatttgaagc
    11221 tgttgaaatg tgtaaaaaac acaaacctga tttagttata atggatatag agatgccaat
    11281 acttgatggt ataaaggcag gcaaaataat atgtaaagaa aatatagctg gtggagtact
    11341 tttattaact tcatttgaag ataaagaata cgtagaaaag gctaaaagta taggtgctta
    11401 tggatattta gtaaaaacag caagtgaaaa atcacttatc ccaaccatag aaatgtgttt
    11461 aaataaggta aatgaatttg aaaaaatgaa aaaagattta gaaaaagtaa attctaaatt
    11521 gaatgaaaga aaattagtag aaagagcaaa aggtatactt ataagagagt ttaagatttc
    11581 tgaggatgaa gcatacacta gaataagaaa attaagtatg gataaaagaa ctacaatgat
    11641 ggaaattgcc aaaatgatta taataggata cgatgactaa tacgataaaa aaaatatcaa
    11701 ctgcacaaac atacttaaat aataacgaaa ttgaaaaaat tatagaagta tcaaaatcct
    11761 tagatttaat ggcttctttt tatgaagctg atgtttttat agatgtacta agtaaatttg
    11821 atgatgaagc aattgttgtg gcacatggta gacctaaaga aaagtcaatg tactgtgaaa
    11881 atgttatagg taaaaaggct ttaattgaaa atgaaccagg agttataaag tgcttaaaaa
    11941 caggagaaag agtaagagat attaaagctt taacacagga atataaactg gtcaaacaaa
    12001 atattcagcc aataacacta gatgacaaag ttttagcagt actaatagtt gaaaaagata
    12061 taagtagtga agttaagaat gagtttaatg ctagtgaaga cgaagaaaat aaaaatttac
    12121 atcagtttat aaaacttatt ggaaataatg actttttagt tgataattta aattcagcca
    12181 tattaatatt tgataaaaat ggaaaattaa aaattaaaaa tcgtattgca actgagatat
    12241 acaaaaatct tgggtttgga tatgatatac aagatatgca ttacaatgat ttagatatag
    12301 aaaccaacac ctttgaaaat atattaatgg atgaaaatta tgatgagaca aatgaagttt
    12361 caataggtaa tatgtttttt aaaataaaga ccatatacat caaagatgat gaatttaagg
    12421 tttgcaaaat agtacaagat ataagtgatt taaagagaaa agaagaagaa ttagttttga
    12481 aaacagtggc tataagagaa gctcatcata gagtaaaaaa taatcttcat acagtaatat
    12541 ctataataaa aaaacaaagt agattatctc aaaatgatga agtaaagcag tgtttagata
    12601 acataataaa tagggtattt gcaatacttt cgactcatta tttactatct aaagaagttg
    12661 ataataatat atctatacga gaaggtatca atttattagt gtcaaatatt caaggtggct
    12721 atttatttag taaggatata gatatacgta taactggtga agattttaaa attagtggag
    12781 aaaaagcaac agcaatactt ttggttataa atgaaatact acaaaattgt tatgaccatg
    12841 cattttcgga tagagaacat ggtactatac aaatattaat aaataaagat aacgactata
    12901 gaaatattgc tattatagat gatggaattg gatttgatag taataatata aataaaaata
    12961 gcttagggac atatataata gatagttata taacacagat gttaaaagga aatatagaga
    13021 gaaaaagtag tgagaatggt acagaagtat taattactat acctacacaa attaatattt
    13081 aaataataac tcatctacaa agtagtagtt ttaattgagc aaagaggctt aaggttatta
    13141 atagattaag ttaattgaat gtatttataa aaattataag tatatattca atttaacagg
    13201 tatttattat aactttaagc ctctttgctt gtttaagaca tacttaaatt ttaggaggtg
    13261 agaaaatgag agaagagtta ttaagtgttg gtattgacat tggaacaagt actactcagt
    13321 taattttttc taaattaata gtagaaaata ctgaatcaaa tttttcggta cctagaataa
    13381 gtatagtaga caaagaaatt atttataaaa gcaaagttta tttcacccct cttatttcaa
    13441 ataatgaaat aaatggtcaa caaataaaag atatagttga aaatgaatat aaaaaagcaa
    13501 atatatctaa agaagatata catacaggag cagtaataat aactggtgaa acagctagaa
    13561 aagaaaatgc aaatgatgta cttcatactt tgagtggatt tgctggggac ttcgttgttg
    13621 caacagcagg acctgattta gaaagtataa tatcaggaaa aggagctgga gctcatattt
    13681 attcaaaaga acattcaact acaattgtaa atatagacat aggtggggga actagtaaca
    13741 tagcctcatt taaaagggga gaagtaaccg atactgggtg tttggatata ggtggaaggc
    13801 ttgtaaagat agataaaata agtaaaaaaa taacttatat atcacctaaa atagataaaa
    13861 tcataaaaga taataattta aatcttcaag taggcacaat ggctactgta gaaaatacaa
    13921 agccaatagt ggatataatg gttgacttat taatggaaag cataggatta aagaaacaga
    13981 cacctttgtt tgattttata ataaccaata aatcaataag tatagatgaa aatattaaaa
    14041 atgtttcatt ctctggtgga gtagctgatt atatttacta tgatggagaa ataaaagatt
    14101 attttgaata tggtgacttg ggaattttac ttgggcaagc aataaaaaat tgtgaatacc
    14161 ttaaaaaagt aaatgtagtt aaatctatgg aaacaataag agccacagtg gttggagcag
    14221 gttctcatac tactgaagta agtggaagta caataactta cactagcgaa aagtttccac
    14281 ttaagaactt gccagtttta aaattatcaa ttgatgaaga aacaggcact tcaaaacaaa
    14341 tcagtgaagc tataaaaaat aaacttaatt ggtttaaact agaaaatgat ttacagaaag
    14401 ttgctatagc catgatagga aagaaaaatc caaccttttt agatatacaa gagtatgcta
    14461 aagggtttgt agatggaatg caacaaatca tagaaaatga tttacctttt atagtaattg
    14521 ttgaaaatga tatggcaaaa gtgttaggtc aagcaatata tgcattatta aattatgaaa
    14581 aagaaattgt ttgtatagac agtgtcaagg ttgaaaatgg agactatatc gacattggaa
    14641 aacctatagt tgaaggtaaa gttttacctg tggtaataaa aacattagtt tttaattaat
    14701 agaaggaggt aagtaaatga ttttaaatac aaaattattt ggacgtactt atcagtttaa
    14761 aggcttatat gatgtaatgg ctaaagctaa tgaagaaaaa tcaggagata aattagcagg
    14821 tcttgcagca gaatcagctg aagaaagaat agcagcaaaa gttgttttat cacacataaa
    14881 attggaggat ataagaaata atcctgcagt ggcatatgaa gaagatgaag taacaagaat
    14941 aatacaagat gctgtaaatg aaaaagttta tgaagaaata aaaaattgga cagtctctga
    15001 acttagagaa tggctattgg attcaaatac tacaagtctt gatataagaa gaatatcaag
    15061 aggattaact tctgaaatga tagcagcagt tgcaaaatta atgtcaaata tggacttagt
    15121 ttatgcagct aaaaagataa aggttactgc tcactgtaat acaactatag gagaacaagg
    15181 aacattatca gcaagacttc aacctaatca tccaacagac gacccagatg gaatacttgc
    15241 atcacttctt gaaggtttaa cttttggtat aggagatgca gtacttggat taaatccagt
    15301 tgatgactct gttgagagtg ttactaaagt attaaaaaga tttgaagaag taaaacaaaa
    15361 atatcaaata ccaactcaaa catgtgtatt ggcacacgtt actacacaaa tggaagcaat
    15421 aagaaatggt gctcctgcag atttaatatt ccaatctata gcaggttctg aaaaaggaaa
    15481 tgaagcattt ggatttaatg gcgacactat agaagaagct agacagttag ccttaaaaca
    15541 cggtacagct acaggaccta acgttatgta ttttgaaact ggacaaggtt cagaattatc
    15601 ttcagaagca catcatggaa tagaccaagt tactatggaa gctagatgct atggatttgc
    15661 taaaaagtat gaaccattct tagtaaatac agttgttggg tttataggac ctgagtactt
    15721 atatgatgct agagaagttg taagagcagg acttgaagac cactttatgg gtaaactaca
    15781 tggcttacca atgggtgttg atgtttgtta cactaaccat atgaaggctg accaaaatga
    15841 tatggaaaac ttagccatac tattaacaac agcaggatgt acgtatttta tgggaatacc
    15901 tcatggagat gatgttatgc ttaactatca aacaacagga taccatgaaa cagcatcact
    15961 tagagaaatg tttggactaa cagcaataaa agaatttgat gcttggatgg aaaagatggg
    16021 attctctaaa gatggtaagt taacagagtt ggcgggagac gcatctgtat tattaggata
    16081 ggaggtattt aatatgaatg agaaggattt aaaagcattg gtagaacaat tagttggaca
    16141 aatggttgga gaattagata ctaatgttgt gtctgagact gttaaaaagg caactgaagt
    16201 tgtagtagac aacaatgctt gtatagatga tataacagaa gttgatataa gaaagcaatt
    16261 actagtaaaa aatcctaagg atgcagaagc ttatttagat atgaaagcaa aaacacctgc
    16321 aagattaggt ataggaagag ctggtactag atataaaaca gaaacagttt taagatttag
    16381 agcagaccat gctgctgcac aagatgctgt attctcttat gtagatgaag aatttataaa
    16441 agaaaataat atgtttgcag tggaaacttt atgtaaagat aaagatgaat acttaacaag
    16501 accagatttg ggaagaaaat tttctccaga aactataaat aatataaaat ctaaatttgg
    16561 tacaaatcaa aaggttttaa tattagttgg agatgggctt agctcagctg caatagaggc
    16621 aaatttaaaa gactgtgtgc cagctataaa acaggggcta aaaatgtatg gtatagattc
    16681 aagtgaaatt ttatttgtta aacattgtag agttggtgct atggaccact taggtgaaga
    16741 attaggttgt gaagttatat gtatgttggt tggagaaaga ccaggattag ttactgctga
    16801 atcaatgtca gcatatatag cttataagcc atatatagga atggcagaag caaaaagaac
    16861 agttatatca aatattcaca aaggtggaac aactgcagtt gaagcaggtg ctcatatagc
    16921 tgaacttata aaaactatgt tagataaaaa agcttcagga atagacctta aataaagtcg
    16981 agggagtgta gaatatgaaa aatgatgtaa ttcgtcctaa tatactagga attaaaataa
    17041 tttcaaatat aagtccagaa atggcacaaa aactagaatt aaaatcacat cacaaaagct
    17101 taggtttaat aactgcagac tgtgatgatg ttacgtatac agctcttgac gaagcaacta
    17161 aagcttcaga agttgatgtt gtatatgcaa aatcaatgta tgcaggtgct gcaaatgctt
    17221 caacaaaact tgctggtgaa gtattaggaa taatagcagg tccaagccca gcagaagtaa
    17281 gaagtggtct aaatgcagta gttgattttc tagagtatgg agcaactttt ataagtgcaa
    17341 atgatgatga ttcaatagca tactatgctc actgcgtatc aagaacaggt acataccttt
    17401 cagaggtagc aggaataaga gaaggtgaag cattagctta tttagtagca ccaccacttg
    17461 aagcaatgta tgcactagat gcagcaatga aagctgcaga tgttaagatg tgtgaattat
    17521 ttgcacctcc aactgaaaca aactttggtg gagcattact tacaggttct caatcggctt
    17581 gtaaagcagc atgtgatgca tttgcagaag cagtaaaatc agttgcagac aatccaacag
    17641 gattttaagg atagttagat ggatgctata ggcttaatag aagtaatagg atatgttgct
    17701 gcaatagaag cttctgatgc ttgtttaaaa tctgcaaatg ttaatattgt aagagttgat
    17761 aaggttggag caggtattgt tactttaaca atatgtggag atgtcggtgc tgtaaaagct
    17821 gctttagaag caggtgaaat agcagcttcc agagttggta cattaagaac atcacatatt
    17881 ataccgagaa tccataatga agtaacaaat gctttattca aaactaaaga acctaaagtc
    17941 tgtgaagttt cagaagataa agaaaaaaac tttgagatta cattggaaat agctgataat
    18001 gtatcgaaaa atttatcaga aaaagaaaat gtaattttag atgtcaatga aaacagtgga
    18061 gatactgaaa atttatatag tgatatagat aaaacaaaca aaaaaattga aacagttgat
    18121 gatagtttaa cagaagctaa tgaggaaaaa atagaaaaca ctaatgataa agaagtattg
    18181 gaacaagtta aaatagaaaa ttccgatttt gatagagatg acctatcaaa gaacagtgta
    18241 aaaaaactta aagctatggc taagaaatta gattcttcaa taacttataa agacttaaat
    18301 gttcttaaaa aagaagaatt aataaactta gttaaaaagc ttagtagaag ggatagatag
    18361 ttatgacact tttagataaa gatttagtat ctatacaaga agtaagaact cttgtaagaa
    18421 gatctaaagt ggctcaagag aaactttcta aaatgagtca agagcaaata gataaaatag
    18481 taaaagctgt tgtagatgca gcatttaata atgctactaa attagctaaa atggcaaatg
    18541 aagaaactgg atttggaaaa tgggaagata aagtaattaa aaatactttt gcatctatgg
    18601 gagtttatga aactttaaag aacacaaaaa ctattggaat aattggtgaa gataaagaag
    18661 ccaatacgat agatatagct acaccagttg gagttgttgc aggtcttata ccttcaacaa
    18721 acccaacatc aacagtaata tacaaaacta taatatctct aaaagcagga aatactatag
    18781 ttttctctcc acatccaaat gcaaaaaact gcatacttga aacagttaat ataataagtg
    18841 atgctgctat aaaggctgga gctccagagg attcaatatt atgtataact aatccaagta
    18901 tagttggaac caatgaattg atgaaacata aagatacaaa cttaatacta gcaactggag
    18961 gagaagcaat ggttagagct gcttactctt caggaacacc agctattgga gttggaccag
    19021 gaaatggacc agcatttata gataaatcag caaatgttaa attagcaatc aaaagaataa
    19081 tagattctaa aacttttgat aatggtgtta tatgtgcatc agaacaatct atagtaatag
    19141 aaaaatctat ggaagaaact gttttaaaag agcttaaaaa tcaaggtgca tatgttttaa
    19201 atgaggagga aagtgaaaga ttagcaaaat tcatattaag agctaatggt actatgaatc
    19261 cacaaatagt tggtaaaagt gttgaggtaa tagcaaattt agcagactta acaaatgtac
    19321 ctaaaaatac aaaagttcta gttgcaagag aaaataaagt tggtcacaat gtagcctatt
    19381 caagagaaaa acttacacca atactagcgt tctatgttga agaaaatgta gaagctatta
    19441 tgaacagatg cagagaaata ttgttaaatg aaggtgcagg tcatacattc tcaatgcatg
    19501 taaatgatga agaattggta aaaagatttg cccttaatat gccagtatca agaatacttg
    19561 tgaatactcc ttcagcactt ggaggaatag gtgcatcaac aaaccttgta ccagcactaa
    19621 cacttggttg tggtgcaata ggtggaagtt caacttcaaa caatatagga ccacttgatt
    19681 taataaatgt taaaagagta gcttatggaa tcaaagaaat agaagaactt ggaagagata
    19741 aatctcaagt tgaaaaatca atgtttgact caatggataa agaagaatta ataaatcttc
    19801 ttgttcaaaa actagtcaaa gaactagcat aactaaaata aacaatgaaa cttatggcta
    19861 tcgctataaa tttctaaaaa tataaactat aaaaaactat aaaattaaat ttttggagga
    19921 taagattatg gcaagtgcaa acgcattagg aatgatagaa actaaaggat tagtaggagc
    19981 aatagaagca gcagatgcaa tggttaaagc tgcaaatgta caattagtag gaaaagagca
    20041 agtaggtgga ggattagtta ctgtaatggt aagaggagat gttggagctg ttaaagctgc
    20101 tacagatgca ggagctgccg ctgccgagag agttggagaa ctaatatcag ttcatgttat
    20161 accaagacca cactttgaag tagatgctat attaccaaag gtatcagctg agtaatataa
    20221 gaataattat aaaatttaaa ttttgattga aaaagaggct atctcaaaaa tgagtcttac
    20281 taataaactc aatgtatttt gaggtagtct tttttataca tctaatagga gatgtgataa
    20341 aatgggtgtt ttaacagaaa acgaagtaaa aagaaaaatt ggaaaaaaca aaaataatat
    20401 aactgaattt tatatagaaa aaggtcaaat aataactcct tctgcaaaat cttatttagc
    20461 tgagagaagt attgagctta aatatgttga taaactttca gatgtaaaca atgaacctaa
    20521 agaagttgta aaagaagtag ttaaagagaa aaaatataga tatactactg tatttggcgc
    20581 taaattggat gaaaaaccag aacatatgac tcaattaaat ggaaatcttt tagtttttaa
    20641 agaccataaa agaataatat tgagaggaaa aatagattct cttgaatcag aaatactaaa
    20701 ggtacagata ctttgtcaga aactagatat gccaaagctt gttaaggaat tgcaagaagt
    20761 attagacttt gtaagaaata taatgagatg tgaagtttta gatgaaaagc taggggactt
    20821 taaactttta ggattaactc ctcaagaact tagagaacaa tctcactatc ctaaaaaata
    20881 ctttggtata ggacatgagt tcccaagtta tgaaatggga gaagtagtag tagctataaa
    20941 tagtataaga agtaatacac gagaggttga acttgcagcc tatgaagcat ttaagggtga
    21001 gtatggaaat gttcacagag aagatttaat aaaggcactt aatagattat cgtcagtatt
    21061 ttggataatg atatataagg ttagaactaa taagtataag tagttggagg acataatgga
    21121 gaatctatta aatgaaatag tggaagaagt aataactaga gttaaaaaag aagcatttat
    21181 agaagttgaa gcatcaggaa gacacgttca cttaagtaga gaagatgtcg ataaattatt
    21241 tggagaagga tatactctta caaaactaaa agacctatct caaccaggtc aatatgcatg
    21301 taaggaaaga gttacaataa ctggtcctaa aggaagtata aaaaatgtag ttgtacttgg
    21361 accatgtaga aatgaaactc aggtagaaat atctttaaca gatggttcaa ctcttggact
    21421 aaaagctcct ataaaacaaa gtggagactt agaaggtact ttaagtatta aaatatcaac
    21481 acaatatggt gaagttgaac ttgataaggg gttaatggtt gctaaaagac atatacacat
    21541 gactcctaaa gatgctgaaa aattcaatgt atgtgataaa gagatagttg aagctaaggt
    21601 gatgggtcaa agacctttaa catttgatga tgtagtaata agagtgagtg acagttttaa
    21661 aacttatatg catatagact atgatgaagc caatgcatgt ggttattcaa aaggaactgt
    21721 tgctaaaata ataaagaaag cgtaaattta acctactata taaattacct ctagggagga
    21781 tttaatatga attatgataa ccttgtagat ttaataacgg aagaaatcta taaaaaaata
    21841 aatagtaatg aaataaaaat atctaataaa cctaaagctg tgattgtatt tgaacaagat
    21901 aacaataaat ttaacttatt aaaagatgaa tttgaaatag tagaatttga taaaagtata
    21961 agggaatgtg aaatagtgat agtttcaaga ctttgtatga gggggctatc gaatatagca
    22021 cttggaaatt caacaagtga tgaagaaaga tttatactaa aaatgattat gaagggcaaa
    22081 gaagtatatg tcctagatga aggaattgaa tacaaaaaat ataaggatac tgcaccaaaa
    22141 gcattatata agaaatttat gtcatttgaa gatgaaatat gtaaatttgg tgtagaaatt
    22201 ataaaagact taaatgttat aactaaaaat aagttaaata taaagaaaga actagaaagt
    22261 actagaggtt ctaaaaaaga agatacaaaa gttttagata aagatgtttt aaatttaacc
    22321 agtgtagaaa gtgtaaaaat agataatgaa tttagtttag atttaaggaa taaaaaactt
    22381 atatcggaag ctgacttaag aaaaccaact ataaatggag ttaaaaacat attagttaat
    22441 aaaaaaagta taataacacc attagctgta gattttatga gaatacacca tctcaaacta
    22501 aagaaagtgt aaggaggtaa ttttatggat ataggaaaag ttgtaggtaa tgtttgggct
    22561 acaagaaaag atgaaaaatt atgtggccaa aaacttttag ttgtaaaaat tttaaaaact
    22621 aaagacacat acaaggaagg cttcatggtt gcagccgata atgcaggagc aggaaatgga
    22681 gacttagttc ttatatccaa aggttcctca gctagagagt cgattgaaaa atctcatgtt
    22741 ccggtagatg caactatagt tggagtagtc gattcgtttg aggtgtttga tgaataaaag
    22801 tataggagct atagagttta aaagcattgc taaaggtata gaagtatcca atgaaatgat
    22861 aaagaaatct tcagttgatg tattgtactt aaaaagtatt tgcccaggta aatttctaat
    22921 aatagtaggt ggagaaactt cttatataaa tgaatgtgta gattatggaa taaaacttgg
    22981 agaaggatat attgtagata acttcgtaat aaatgcaatt tcacaagaaa tattagatgg
    23041 atttaaaaat aagtatcaaa agctagatag tatagtctct ataggcgttg ttgaaagtag
    23101 taaagtatgc gctggaataa aaatgcttga taagacttta aaatctggcg acttagtatt
    23161 agtaaaactt caattatctt ttgctatagg tggcaaatta gtatatatag tagcaggaga
    23221 tttaagtagt ttagaatatg cattaaaaga aagtgaaaat gtagtaagag aaaaagaagt
    23281 aatatataag actgtaatac catcagtaga cagtcaaata ataaaaagtc taataaaata
    23341 atggggggaa ttatgagtat aaacgaaatt ataatctatg taatggtagt cttcatggta
    23401 cttggagcta tagataaatg tataggtaac aaatttggtc ttggagaaca atttgaagaa
    23461 ggtataatgg caatggggtc acttgcagtt gcaatggttg gtgttatatg tttagcacca
    23521 gttcttgctg atgtattaag accaattgta gtaccagtat ttaatatact tggagctgac
    23581 ccagctatgt ttgctggaag tttattagct aatgatatgg gtggtgcacc acttgcactt
    23641 gaacttgcga aagaccctaa tgctgggtta tttggtggat taatagtagg ggctatgatg
    23701 ggacctacaa tagtatttat aataccagtt gcgcttggaa taattgaaaa agaagaccaa
    23761 aaattcttag caacaggaat acttgcaggt ataataacaa tacctattgg agcattcgtt
    23821 ggtggattag ttgcaggatt cgaggtaatg atggttctta gaaacttagt gccaataata
    23881 atatttgcag tagttatagc acttggatta ttaaaattcg aaaatgctat gataaaagga
    23941 ttcacatact ttggaaaagg tgtagtaata gttataactt taggacttgc tgctgccata
    24001 gttgaagctt taacaggaat agttgtaata cctggaatga aaccaatcag tgaaggtata
    24061 gaaatagttg gagatatagc aatagtttta gcaggagcat tcccattagt gtttgttata
    24121 acaaaggtat ttaataaacc attaatggct ttaggaaaag ttcttggaat gaatgatgta
    24181 tcagcagcag gccttgttgc aagtttagct aactgtatac caatgtttgg tatgatgaaa
    24241 gatatggaca atagaggaaa aataataaat gtagcattct ctgtatcagc agcattcgta
    24301 tttggagacc acttaggatt tacggcagga tttaattcta atatgataac accaatgata
    24361 gttgctaaat tagttggtgg tataacagca gtattgttag ctatggttat cgcaaataag
    24421 acactaaaga aggagaatgt ataagatgga tatatcaaat atagataaaa atttaattga
    24481 aacattagta agacaaataa tagaagaaaa aataagtgga actaaagaca cagtagattt
    24541 tgttagaaat aaagacatta gtggaataac ttctataaag ttaccaactg taaaagttag
    24601 tgaatctgat agattagata cagggaatcc aagtgatgta gtttacacaa aagatttatt
    24661 tacattagag gaaagcccaa gattaggttg tggtatgatg gaaatgaaag aaacaacttt
    24721 tgattggact ctaaactatg atgaaataga ttatgtaata gatggaactt tagatataat
    24781 aatagatgga agaaaggttt ctgcttcttc aggtgaatta atatttatac caaaaggaag
    24841 taagatacaa ttttctgtac ctgattatgc tagatttata tatgtaacat atccggcaga
    24901 ttgggcatct caaaattaat aaaaatcaag ttaagttaat aataatcaaa taaataattt
    24961 aaaatacaaa ctcttaaata agcacacata tttaaaaaag ctatgaatta aaattcatag
    25021 ctt
//
LOCUS       DAMJYX010000048        24986 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJYX010000048 DAMJYX010000000
VERSION     DAMJYX010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.048"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            50..1906
                     /locus_tag="PC479_002075"
     CDS             50..1906
                     /locus_tag="PC479_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2804618.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1989..2229
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2295..2530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2615..3832
                     /locus_tag="PC479_002076"
     CDS             2615..3832
                     /locus_tag="PC479_002076"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2804619.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3949..4431
                     /gene="cdeM"
                     /locus_tag="PC479_002077"
     CDS             3949..4431
                     /gene="cdeM"
                     /locus_tag="PC479_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2804620.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4695..5960
                     /gene="hisD"
                     /locus_tag="PC479_002078"
     CDS             4695..5960
                     /gene="hisD"
                     /locus_tag="PC479_002078"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2804621.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6163..6915)
                     /locus_tag="PC479_002079"
     CDS             complement(6163..6915)
                     /locus_tag="PC479_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2804622.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7033..7302)
                     /locus_tag="PC479_002080"
     CDS             complement(7033..7302)
                     /locus_tag="PC479_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804623.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7777..8871
                     /locus_tag="PC479_002081"
     CDS             7777..8871
                     /locus_tag="PC479_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2804624.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8873..9823
                     /locus_tag="PC479_002082"
     CDS             8873..9823
                     /locus_tag="PC479_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2804625.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9816..11000
                     /locus_tag="PC479_002083"
     CDS             9816..11000
                     /locus_tag="PC479_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804626.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            11013..12509
                     /locus_tag="PC479_002084"
     CDS             11013..12509
                     /locus_tag="PC479_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2804627.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12532..13482
                     /locus_tag="PC479_002085"
     CDS             12532..13482
                     /locus_tag="PC479_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804628.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13503..14465
                     /locus_tag="PC479_002086"
     CDS             13503..14465
                     /locus_tag="PC479_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2804629.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14837..15469
                     /locus_tag="PC479_002087"
     CDS             14837..15469
                     /locus_tag="PC479_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2804630.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15880..15972
                     /gene="kdpF"
                     /locus_tag="PC479_002088"
     CDS             15880..15972
                     /gene="kdpF"
                     /locus_tag="PC479_002088"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2804631.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            16013..17695
                     /gene="kdpA"
                     /locus_tag="PC479_002089"
     CDS             16013..17695
                     /gene="kdpA"
                     /locus_tag="PC479_002089"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2804632.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17720..19777
                     /gene="kdpB"
                     /locus_tag="PC479_002090"
     CDS             17720..19777
                     /gene="kdpB"
                     /locus_tag="PC479_002090"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2804633.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19796..20422
                     /locus_tag="PC479_002091"
     CDS             19796..20422
                     /locus_tag="PC479_002091"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2804634.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20659..21567
                     /gene="cysK"
                     /locus_tag="PC479_002092"
     CDS             20659..21567
                     /gene="cysK"
                     /locus_tag="PC479_002092"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2804635.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21594..22184
                     /gene="cysE"
                     /locus_tag="PC479_002093"
     CDS             21594..22184
                     /gene="cysE"
                     /locus_tag="PC479_002093"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2804636.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22344..22532
                     /locus_tag="PC479_002094"
     CDS             22344..22532
                     /locus_tag="PC479_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2804637.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22586..23455)
                     /locus_tag="PC479_002095"
     CDS             complement(22586..23455)
                     /locus_tag="PC479_002095"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2804638.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23652..24896)
                     /locus_tag="PC479_002096"
     CDS             complement(23652..24896)
                     /locus_tag="PC479_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804639.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
BASE COUNT         9624 a         2673 c         4506 g         8183 t
ORIGIN      
        1 taattaagta gtattattat aaataacatt gaaaggtagt ggttagaata tggataccca
       61 taataaatat gtaaatttta taaaaaatat acctgtacct tttttatatt gtagaattgt
      121 aaagagacaa gaagatatag aatatcgagt agaatatata agtaagggta tgggcaaagt
      181 attacaatta gaagaaggta tttgtgataa aaatatacta gatgtattgc ctgtatttaa
      241 gtctaaaaaa tatttcaaag agttgttctc aaatgaagtg gattgtataa aaaggtacat
      301 tccgacactt aaaaattgga taaatataaa aaaacagata ataggggatt catacataat
      361 tttgtatttt ggtaaaattg tatttgatta tagacagata atcgattcct ttgataaaaa
      421 ggaaaaagta gcttatatta aagacgaaga aggcacatat atagattgca gtgaaaattt
      481 aataccaata ttaaataaca acattaaaac aacgaaagat atctttggta aaaatgatat
      541 agaagtatgg ggagagaata caggaaaatt atttagagat gattatagag aaggagtatc
      601 tagtaaaaaa agatttttac aaaatctttt tgaatatgaa gaaacatttt ttatggttga
      661 aaaatatttt ttgtatgatg aagacgaact tttaggtact atagggatag tagacaatat
      721 aatatatagt ggttacagta atagaaatta taactcaaaa gatttaatga agatgataga
      781 acactcaatt ccagagaata tgttttataa agatgtgtat ggaaattata ttggatttaa
      841 ttcaggtttt ttgaatttag cttgtatgaa taaagaagaa ttattaggga agaatagtta
      901 taaaatatca gaagaagaag ctttaataga taagatattt gaaagtgata agggtgtagt
      961 tgaaaataaa aaagtagtta catttgagtt aaatataagt atgaatgatg aaaacaagtg
     1021 tattgagatt acaaaaaggc cgttttttga tagctatgga agtgttattg gaataattgg
     1081 aacagcaagg gatataagta gaagaaaaag attagaagag gaaatggata aaactaggat
     1141 ggaatttttt gcaaatttat cccatgaact tagaacgcct ataaacttaa tatcatcttc
     1201 attacaagtg atagaaaaaa aagaagcaga tttaatagaa tcaaatgata ctttaaaaag
     1261 aaatttaggt ataataaaac aaaatggaaa tagaatatta agattagtaa ataatgttat
     1321 agattttaca aaaatgcaat caggatattt agattttaaa ccagaagaat ctgatataat
     1381 tgcatttata gaggagatat gtatgtctgt agctgatttt gcaagccaaa ataatataca
     1441 acttacattt gatacagaaa tagaagaatt ttcaatgtta tttgattcag aaaaattgga
     1501 aagaataata ttaaatttac tttctaatgg aattaagtac aataagaaag atggaaaaat
     1561 aaatatattt ttatatgtga aagataatgt atttaatatg aaaatatctg atagtggaat
     1621 aggaatacca aaagaaaaaa tagataaaat atttaatagg tttgaacaga ttgacaatga
     1681 actttcttac agagttaaag gaagtggtat tggactttca ttagtgaaat ctttggttga
     1741 gttgcatgag ggaagtatat ctttaaaaag tcaacttggt attggaagtg aatttatagt
     1801 ttcattgcct gttagaagta aaaataatat tgaaaaatat aatcataaaa gagaaatcag
     1861 caatgaatta agtaaaaaat tagaaataga gttctcggat ttataagttt aaatataaaa
     1921 aataaaattt taaaaaagta ttgacaattg atttagaagg gtttaaaata aattacaaca
     1981 taataaataa aaaggcgatg aataggaaga gtaaatctaa ttcgattacc agagagaaga
     2041 gtccgttggc tggaagactt tttaaattag agggattgaa aactacctag gagctacttt
     2101 ctgaagtcat ctgcattagt agattttagt aagaatagtc gttagctgcg ttaaagtgta
     2161 aagtgagttt tcatgacata tgaaaactaa attaggtgga accgcgggaa taatactctc
     2221 gtccttatgt tataaggatg agagtttttt gttatttaaa aatataaaca gtaatcataa
     2281 taattataga aaatataaaa gcaatgaata ggaagagtaa atttaattcg actatcagag
     2341 aggaaagttc acaggctgaa agactttcta agtttgagaa attgaaaact acctaggagc
     2401 tgtggtctga agttacttta aaaagtttta gtaagactaa ccgttaactg cgttaaagtg
     2461 taaagtggat tttatcaata aatgataaaa tcaaattggg tggaaccgcg agtatagact
     2521 cgtcccttta tatagggatg agtctatttt tattgcaata aaagattgaa aataaaaaaa
     2581 aatcggaaaa ttataaattt aggaggaaaa taaaatggag aataaagtga aaattggagt
     2641 actaggatat ggagttgtag gaagtggact tatagatata atagataata ataaagaaaa
     2701 aagaaatata gaaatagttg gaattttagt taataatttg gaaaaacata aggataaaaa
     2761 atattctaat ataataacta ataatataga tgatatattt aataaagata tagatatact
     2821 tgttgaagtc atgggtggtt tagaaccatc attatcttat ataaaaaaag cattaaacaa
     2881 taaaattcat gttgttacag caaataaaga tttgttagct gaatgtggag atgagcttgc
     2941 aaaattagca agtgaaaata aagtaagtat taaatttgaa gcttcagttg caggaggtat
     3001 accagtatta aaaccaataa tagaatcact agaaggaaat aatatagata gtataaatgc
     3061 aattttaaat ggaactacta attttatatt atctaaaatg tatgatgaga atctatctta
     3121 tgatatggca ttaagacaag cacaagaatt aggttttgct gaagcaaatc cagagtcaga
     3181 tgtattaggg tatgatgctg ctagaaaact atcaatatta tcaactctag cttatgacaa
     3241 tagagtatat tggaaagatg tgtatttaga aggaattaca gatatagatg aaaaagatat
     3301 tgaatatgca aaaaaattaa attgtaaaat aaaattgatt ggtcaaagta agtatgaaaa
     3361 tgataaagta agtgcttttg ttagacctgt tttagtagaa aaagataata tccttgctag
     3421 aatagacaat gagttcaatg ctgtcatagt gaatggagat tcagtaggag aagtttcttt
     3481 tgtaggtaaa ggagctggaa gtttagcaac aggaagtgcg gtttattcag atgttataga
     3541 tataatagat aatagagttt caagtataga ttcttttact aaagataaaa tacaagttaa
     3601 taaaatagtt agagaaaagt gtggagcact tcttagattt aaaaaatgta ataaagatga
     3661 aattctaaat atagttgaga attgtttagt aaaattcgat attttaaatg atgatgatga
     3721 acttgctatt atggtttatg ctgattctga atatgaaata aacaacagtt tatgtctaat
     3781 aaaagataaa ggatactgcg aaaaaatgaa taaaatgttg aaaataagtt aaaaaagaaa
     3841 aaatataagt ttatatgtaa catattttga cgattttaat actatatagt atgagatgaa
     3901 acaaaacatc tcataaaaaa attaaaatta aagggagaga tatgttatat ggaaaataaa
     3961 aaatgttatt cagaagattg gtatgaaaga ggagaatcta cagctaaatg gttccaaaat
     4021 gatagagaag aatatgaaag agaagcatat gatgaagata gagaaagaag aggttcaaac
     4081 tgtggatgtt cagattcagg agaaaataga cctagaaact gtgaaagatt tagaagagaa
     4141 gctgagataa gagaaagaga agcaagagaa gcattctgtg aatcttcaga gaaaaagaaa
     4201 gaggcattag catatgaatg tgaagctaga aaattatggg aagaagcaga aaaatactgg
     4261 gatgaatatt caaaatacaa ctataaagga atcgaatatt tagcagaagc tgctagatta
     4321 tttgatgaag gtatggaatg tgaagctaga agaaatggaa ataatggagg aaacaataat
     4381 aattgttgcc ataaatgcca taaatgtaat tgtaactgct gtagaaaata aaaaaattaa
     4441 ttgacaaatc aatagttaaa atatatcata ttatgtataa taaagagaaa attcagaaaa
     4501 aaataatgat tagaagaaat ataagtatat ttattagaca aatagtgctc agttagtgag
     4561 gtgagtacaa tatgattaaa ctcccatcct ctagcttttg atagagggtg ggagttttta
     4621 tttatttata tatcttaaat gaggataata ttgtaaaata aatgagtatg agattgtaaa
     4681 attaggaggt aaaaatgaaa agtattaaaa catcaagtat gaaagaaaag gtagatgaaa
     4741 agatattaag agagcaagta agttctataa tagaagatat aagaaataat aaggatgttg
     4801 cacttaaaaa atataatgag aagtttgata gaaatacaag agatgaattt agaattacta
     4861 aggaagaaat aaaggaagct tataagcatg tagatgacga atttataaat aacttaaaga
     4921 ttgctgccaa aaatataaga gaatttgcta aagctcaaaa actaagtttt gaaaaccctt
     4981 ttgaaaaaga aatttatcca ggtgtaatct taggtcaaac taatattcca atagagtcat
     5041 gtcttgcata tgttccagga ggtcagtatc cattgttttc aacagctctt atgcttataa
     5101 tacctgcaaa agtagcagga gtaaaaagaa tagtggcatg ttcaccaact atgaaaaata
     5161 ctgaaaagat aaatcctaag acattggtag ctatggatat agcaggtgct gatgaaatat
     5221 atgcaactgg aggagttcaa gctatagcag catttacata tggaacagaa aagattaatc
     5281 cagttgacat aattgtagga ccaggaaata agtttgtaac tgaagctaaa agacaatgtt
     5341 atggacaagt agggattgat tttgtcgctg ggccaagtga agttcttata atagcggatg
     5401 aaacttcaaa tccagtttat attgcagctg atttattagc acaatgtgag catgatttaa
     5461 atgcaagagg aatattattg acaaatagct tggaagtagc acaaaaagtt gaaaaaaata
     5521 tagaaagtat gctaaaagat ttgccaacaa aggacatagc atatagttca tgggaaaata
     5581 atggagagat aatattggtt gatgatatgg aagaagccat taaaataagt aatttttatg
     5641 caccagaaca tttagaagtt gcagtaaatg agtataatga tatttgtgat agattaacta
     5701 actatggttc tttatttatt ggaaatcttt ctgctgaggt gtttggtgac tatgtttcag
     5761 gtaccaatca tacattacca acacttaaag catcaagata tactggtggt gtttgggttg
     5821 gaacttttat caaaacttgt acaaagcaga tatttaatga aaaagctata caatcattag
     5881 cacctgtagc tgaaaagctg gcaaaagaag agggattata tgcacatgca aaggctgctg
     5941 aggttaggtt taaaaagtag taaaaaattt tattttggca gtcgagactt agtattagac
     6001 tgagataagg aaaacgctga gaacctgaat aaatactata ttttgtgggt ctcggcgctt
     6061 cttttatatc tgtatataat agaataatac tatctgtgtg tttaaatact aacttaatat
     6121 ttcttttcta aataatgttt atgaaatagt taatattttt agttaatact tgaaaaaaac
     6181 ttttgctaac tgtgcaaatt catctgcatt tgtcaatata gcagggtgtc ctccctgact
     6241 aaaaatgtaa atacttgctt ttgaaataaa agttgccatt tgcttgtact cttcttcaag
     6301 attatgacga cacatttcgt cttccttact tcctgtaaat aaaattggca tttctaattt
     6361 acacaattct ttatgaaaca aagggcgttt ctcgtttgca cattgcaata gagcttttgt
     6421 atcaagatta acaacattct cccaatcttt tccatgacac cattcataga attttcgtgc
     6481 ttgaatatct tgctttgatt tttcccttcc agtgattaga ttatctatga agttatcatt
     6541 aagagtacgt ccatcaaagc tatctgctat aattgcttca actaaatcgg gacgttctaa
     6601 tccagtatta acagctgccc atgctcctcc acttgttcca attaagccaa ctttaccaca
     6661 attcaaatgt tcaatcagtg ctattgtctg taaggcttca tcatgccata tgtcaggaga
     6721 aaattgagaa actctatccg actgtccatt acctaaaaaa tcaattaaga tacatctaaa
     6781 attttctgca tataatggca caagtgcttt aaacatatta gaagaaccag tattaccatg
     6841 caggaaaatt atcggtttac cttgtccata ttctttataa aaacaagaat taccttgata
     6901 ctgaaaatat gacatatatt ttcctccttt ttatttgtta agttaattat aacaatatag
     6961 gtatgaatga aaaacctcat gttctttctt agaataaaaa tgtgccacgc ataaaacgtg
     7021 acacatttct aattataaat tttgctgttt aagtattctt tgtatgcttt tctctgtcaa
     7081 aaagtatttc tctgataact gcttgatact cattccattt tggaaattta tacagatttg
     7141 agaattacga gaagctaaga cttgtttggt gtttgtgttt tcaccccaat gctttttgtg
     7201 ctcttgcttt ttgggaatat aaatcacttt accatctata tattcttgca cttgctcaat
     7261 cagttcttta ggcagtaagt caatcgaatt ttgatagctc attttgcacc tcctaaataa
     7321 ttattcttta ggattagcaa aggctactgc cactcacaat taaaaataaa attttttatt
     7381 atgtacaata gcctttgcta tgcacaagtt ccaattccca taaactaaat actcctttct
     7441 ggtttgaaaa attcatctga acattgattt cgcttttaag atagagttca atgacaaatt
     7501 tacattttta ttatatatat tcagatagat aattgcaact gtctttgtat gaggatattt
     7561 tattatagtc aatactataa tatgtattta tatcatcaaa ctaatatgaa cagttgaaag
     7621 gaatatgaac aataaaactt agtcaggaag aataattatg aagcagacaa tagagaaatt
     7681 tgattttaaa gttttattga aaatttattg acaaatttaa cttgtaaata ttatactaaa
     7741 cttaataaat aaagtttata aagtaaggtg ttgtctatga aaaatatagt tggaaaaccg
     7801 caaacgataa aaaaagtaaa tgaagattta ataaaaaaca ttataaaaaa tgaaggtcct
     7861 ataacaaaac cagaaatttc aagcattact gaattgagtt tagctacagt taataagatt
     7921 gtagagcaat tagctttgaa aaatgaagtt aaagtaagtg gactaagtga atctactgga
     7981 ggaagaagag ctcaattatt tgaaataaat gcaaacttag aacacataat agctttatat
     8041 tattatagaa attgttgtat aggggtagtt gccaatttat taggtgaaat aatttatcaa
     8101 gaagaattta atataagaat ggatacgtat gaaaacgtta accaagatac tttttttgtt
     8161 atagatactc taataaaaaa ggaaaaagga aacaatataa gggctattgg aattggtgtc
     8221 ccaggagttg taaaaaaagg tgtagttaaa aatataccaa ctataaaaag ttgggaaggt
     8281 ataaatttag aaaatttaat aaagagtaaa tacaatacta aagtgtttat agaaaatgat
     8341 acaaatatta ctactattgg gatttatcaa aaacagtata aagacaaata taaaaatatg
     8401 gctcttatgt atttagaaga tggtattggt tcaggtatag ttataaatgg tgaattgtat
     8461 ttaggttcta caaattttgc tggagaatta agttatctaa atatagaatt tggtaagtat
     8521 aactctaaca acttaaatct tgaagattat attatgagtt taagaaaaag atatttagaa
     8581 agtaaagata ataaatttaa gaaagaaatt ttatctataa tatcaaactt tataaggact
     8641 cttgtatgtg tcctaaatcc acaagctatg attatacagt gtagtatatt aaccaagagt
     8701 gacatggagt ttatagaaaa agatgtaagc aataatgttg gagaagaaaa ttttcctaaa
     8761 ttaataaaag tagactcatt gagagaagga agtataaatg gagtaattag tatgtgttta
     8821 aaagaaactg actatcaata ttccttatct aataaaaaag gaggatatta atgtgaaata
     8881 tttggtttca atagatggag ggggaacaaa aactaaattt tgtgtaagtg acttagatgg
     8941 aaacatactt aaagaacata ccactggttc aactaattat aagtcagttg gaattaaaaa
     9001 aacatatgaa aatattaata atggatttaa aaaaatatta aaagatttat atatcgatta
     9061 tgacgatatt gagtacactg tatttggtat atctggttgt gattctccta atgattataa
     9121 gattataatg gatgaaatat taaaaattgg gattaacaaa gaaaaaatat atctggcaaa
     9181 tgatgcagtt ttagcttttt atgcacaagc tgattcccct ggcttagtaa ttgtggctgg
     9241 gacaggttca attatactgg gaattaaaga agatggagaa atatatagag ttggtggatg
     9301 ggggtataat tttagtgatt tgggttctgg atatgatata gggagaaaat tattaaagaa
     9361 agtattgctt tattgtgatg aatgtcatga gtatagtgat ttatttagtt gtgtcttgga
     9421 cttctttgga gcaaatagtt ttgaacaact tgcatatatg attactgata taaatgataa
     9481 tgttgaaata gcaaacttag cttcattagt gattgattgt gccgaacaag gtgataaatt
     9541 ggctattgaa atattaagag aatcatcaac tgaactatct aaattagctc aagtaatatt
     9601 gagaaaaata tctaatccaa tgaaaatgga taaaagcgaa ataaatatag ttttgtctgg
     9661 tggtacatta aataaaactg tatatgctca aatgttaatg aataatttaa aaacagaaaa
     9721 tacagataaa aatttaaaat ttatatctca agaaaatcag ccagtatatg gggggataag
     9781 acttgctatg gcattagcta ataggagggt caagcatgga tagaaaaaag gtaatagtac
     9841 aaatttttac tggaggtttt aaggctaaag aaataaagtt taatgatata aaatgtaaac
     9901 tactagaatt attggactgt attgatatag aaaaggttat catgggatgg agtgtagatg
     9961 aagaattata tgtagaaact aaaaaactat taaataaata taatgttgaa ctatatcttt
    10021 ggttgccatg tttttctgaa ataggattac ttgaaaaaag taatttacta attgattata
    10081 tggggaagga agtcaagggc tatgctttac aagaggatga aaattttgag ttttattgcc
    10141 caaataataa ggaaaatata aaaaatataa agaaaatata tagtacttat tttagtgata
    10201 ttaaatttga tggaattttt ttagataaaa taaggtatgg ttctttttcg aataaactaa
    10261 atggtgtttt taattgtttt tgtaaagatt gtttaaatat atataaaaaa aataatattg
    10321 ataaagagtc gttaatgaga gaaatgaata atgtgtctaa gggagttata gataatgaag
    10381 atactccatt tggtataaca gaatacatta atggaaaata caaatttaaa aataaaatat
    10441 gggaagattt ttttgaaaca aaaaatcaaa atatatacaa ggcattatta gagataagta
    10501 gttattttaa aagtaaggga ttgaaaatag gtatagatgc tttttctcct tttatttctt
    10561 actttacagg tcaagattta gaaaaattac aagaagtagc agattttata aaaccaatga
    10621 tgtatagaac tacaaaagct ccagctggtt taccatttga atttgaaagg tttattgatg
    10681 aatccataca taagaataaa gagattggaa aaagaaattt cttagatatt ttaggaatta
    10741 aggagtatga aggagatgta tatcctatag aatttgtact agaggaaatc gattttatga
    10801 caagaaatct taataaagat atatatgtag gagttgaaat aaataaaaag gaaattatat
    10861 ctcctgttac acctaagtac ataagtgaaa atatttcaaa tataaataag acaacagtaa
    10921 aaggatatgt tttatcttgg gatttgttgt cagcaccaaa agaaaatatt gatgaaatta
    10981 taaaatggtt tcggaattag tgaggtgata aagtgagtaa gatattggaa atacaacaaa
    11041 tatataaaag ttttggaaag gtagatgttt taaaagggat aaattttgag ttagaaaagg
    11101 gagaagtaag agctcttttg ggagctaatg gagcaggaaa atctacactt attaaaattt
    11161 taggaggagt tcattctcaa actaaaggta aagtaatttt gactggtaat gaaataatgt
    11221 ttaaaaatcc acatcattct aaagaaaatg gtattagtat aatttatcaa gaattaagct
    11281 tgataccaac gcttacagta cttgaaaata tttttcttgg tagagaaatt ttaaaaggaa
    11341 gtgtgttttt aaacaagaaa gacatgcttg aggaatatat gaaaatttgt gaggattttg
    11401 attttaatat agacccacat atagtagttt ctaatttaag tgtagcaaac caacaaatgg
    11461 ttgagataat gaaagctgtt tcatgtaatt ctgaaattat cataatggat gaacctacaa
    11521 catcacttac taataatgaa aaagatgggc tatttaaaat aataagtaga ctaaagtcta
    11581 aaggtaaaac aatagtctat atttctcaca tattagagga agtttttaaa gtatgtgata
    11641 gtgcaagtat aatgaaaaat ggaattatga ttggaactta tgatgtagag gatttaacta
    11701 aattaaagat aactcaactt atgacaggaa atgataatca caaacttatt gagaagaagg
    11761 attatacatt tgcaaaatat gaagagccac cagtactaga agtaaagaat ataagctggt
    11821 ctaacaaaat aaaaaatgtt tcatttaaag tttatagagg cgaagttgta ggtcttgcag
    11881 gtcttgtagg ttcaagaaga actgaaatta taaatttgat atttggtata gataataaag
    11941 atagtggaga tatttttatc aatggtaaaa aagtagacat aacttctcct aaggttgcta
    12001 taaaaaacaa aataggattg attcctgaag atagaaaaaa tctaggatta attttagaac
    12061 aagctatata cacaaactca tcatcagtac aaattgataa attcaagaaa tttttgttta
    12121 tagatagaaa taaagaaatt gagttttcta aaaaaggtgt taaggaatta ggaataaaag
    12181 ttagtgatgt aaagcaagaa gtaaaagagc ttagtggtgg aaatcaacaa aaagtagtag
    12241 tttctaagtg gttagagaaa gatttagatt taataatata tgatgagccg acaaagggaa
    12301 tagatattgc tgcaaaagaa gatattttca atacaatcaa agaattttca agtaaaggaa
    12361 taggagtaat atttatatct tctgatttag aagaagtaat aagagtaagt gatagaattt
    12421 tagtaactag agaaggttct attgtagatg atttaaaaaa tgagaatata caggttcaag
    12481 atataatgaa cagtatattt aatgtataaa aaatgaaaat ggaggactga gatgaagaaa
    12541 gcttttgaga agttaaattt aagagattat ggagtattat taggtttttt agctttatgt
    12601 atagctataa gtattgcaac tccatcattt ttaggtaaac aaaatatact taatctttta
    12661 cgacaatctt ctataattgg aataatttca gcaggtatga cttttgttat aatatctgga
    12721 aactttgata tatcagttgg agcagtagca gctcttgcag gtgctataac aatgaaattt
    12781 gcgactggtg gaactaattt gtttgcatcc atgttcttag gtgtagcgat ttgtgcagta
    12841 ataggattaa tcaatggtat agtagttgca aagataaatg taccatcatt aatagccact
    12901 atggctatgg tcactattgt aagaggattg ctgttgatgc ttacaggagg atatccaatt
    12961 actgaaaata taccaatact tgattacatt ggaaatggat atttgtttaa tataccaata
    13021 ccagtagtaa ttttctttat agtggtgtta gcttcgttta ttgtacttaa taaaactaag
    13081 tttggtagat atgtttattc tgtaggaggg aatcaagaag caagtaaact taatggtata
    13141 aatgtagatt ctcacaaagt gaaagtgttt attattaatg ctgtacttgc aggaatagct
    13201 ggaatcgtac taacaggaag acttgggact gcaactgcga ttgcaggtga aggatatgat
    13261 atggatgcaa ttgcatctgt agtaatagga ggtacatcag tggcaggagg ttctggttct
    13321 gtacttaaaa ctgttatagg agtattactt atgagtgtta taaacaacag ttttaatctt
    13381 cttggaatag atgtatattt ccagtacata tttaaaggat taataatttt agcagcagtt
    13441 ggatttgatt cttatagcaa gaaaaaactt gcttcaagat aaaaaataag ggggattttt
    13501 ttatgaaaaa attaatgaaa catttagcat tgttgctatc acttgttatg atatttggtt
    13561 tagttggttg ttcaaatggt ggtgacaaag aaaaaaatgg agaaaagaaa atagcagtat
    13621 tactaccagg ttcaacaggt tactttgtag caactaaaca aggtatagat gcaaaatcta
    13681 aagaattagg tgtaagcgtt gaatatgcag atgctcaatg ggatgctagt aaacaattat
    13741 cacaagctga agattttatg gctaaagggg tagacatgat tgcattatgt ggagttgatt
    13801 ctgctgtaag tgagagaata gttaaagctg ctaatgattc agacgttcct atagtagcat
    13861 ttacaaattc tataggaagt aacccaactg gagaatttaa aggtttagtt acatatatag
    13921 gacaaaatga ggaagaaaca ggagcattaa ctggaaaaat tgctaaaaac ttattaggag
    13981 aaactggtgg gaaagctgta ttaatagaag gagtaccagg aactactcca caagtattaa
    14041 gaaaaaaagg tcttgaaaaa gaattaaaag atagtaatat agaaatagtt tataatcaaa
    14101 cttcaaattg ggagaaagag caagctttaa aagtaacaga agatttaatt caaaagaaaa
    14161 ctgattttaa tgttataata tgtcaagatg ataattctgc tacaggtgca ggtcaagctt
    14221 taaaagatgc tggtcttaag gataaagtta aagtcatagg tttgggtgga agtaaagatg
    14281 gattaaaagc tataactgat gggttaatag atggtacaac ttatatgtct gctgtagaag
    14341 aaggtggatt agtcatagaa aaggcatcta aattccttaa aggtgaaaaa atagaacctg
    14401 ttacacaaat taaacaagtt gaagttaaca aagataatat ttcagagttc aagggtgaat
    14461 ggtaaattgt catagataat aaataagatt tttagagtta ctttagaaac tatataaagt
    14521 aactcttttt ttaatttaat ttagtgataa attattgaat agtatttcta aacaaataaa
    14581 aaattaatat aaacaaagac ttaattatac taaattataa gtaaaactaa tataaactta
    14641 tggaaaaaag gaaaacgtct aaagtattgg aattactata gtgtaggtgt agtattaaaa
    14701 aaacataaaa aaacataaaa aaattgttga aaaaaatata gaaattttgt ataattaaag
    14761 atttgaaaag tgcggtatac aaagccaatt cttttgtata taatatatat aaattcagaa
    14821 aaaggaaggg atatgtatgg ccaggcggtt atttttagaa aggattggac ttatattttt
    14881 aggttcagca atattagcat ttggagttta taatttttat tatttaaaca acataacaga
    14941 aggtggagtg ttaggtattt tactactgtt aaaaaattta ttcaatatac aaccagcaat
    15001 agcaaatgtt gtaatagatg gtttactact tttagtagga tataaatttt tcggtaaaaa
    15061 attccttata tattcaatag tagcttctat aactttttca gtgttatatg acttatttga
    15121 agctatagga cctctagtac cacaatcaca aaatatgctt ttatcaacta ttttggcagg
    15181 tgtaactgtt ggaactggtg taggtatagt agtaaaagca ggttgtgcat caggaggaga
    15241 cgatgcttta gcattagtaa tttctaagac tacatctttg aacatagggc aagtttattt
    15301 agcaacagat gttattgtat tgttgttatc attattttat ctatcagcgt ttgatatctt
    15361 ttattctttg attgctgtta caattagtgg caaggtaatt gattttattt attatcatgg
    15421 taaaagtttg gatatggata taagtaatga tatagttcca gaatgttaat ataaattaaa
    15481 taactttaga ttttattagg ggtggcagat ttgccacctt ttttgtgtct aaatctttat
    15541 agaaagtata atcccatcta ggtttttgca aatctcaaat atagaattat gttggaatga
    15601 ctgggaatac gattgctaga tgtgcatttt aaaaacccaa ttttatattt atgaaatact
    15661 aatcattaat ggatgtttct taatcctatc tttataccta aaatgataag ataaatgaag
    15721 tagcaataca aaactaaaat ttgtaaaaga gattaaatta aatggaggag ctaaaatgtt
    15781 ggatatagtt atgatagcat tgttattcat aggatttgtt tccatgaagt tacttgctga
    15841 ttggtgtggg aaacaaactg aaaaaaaata atgtaatgta tggagggatt acctatgtgg
    15901 tttctggcag ttgtaattat aatgttaata atatatttgg tatatgcgct tattaatcct
    15961 gaaaaatttt aaacagtata aggctaataa gtaagttgaa ttggggggaa caatttttat
    16021 ggtgcagata gtaatagtgt tagcgatttt tatgatttta gtaataccta tgggcaagta
    16081 tttatatcat atagctacta atcaaaaaac atttggagat agactatttg ataaagtgga
    16141 caattttata tataaggtat gtagtataga taagaaaaaa gaaatgaact ggaaacagta
    16201 tgcattagct ttattattta caaatgcagt tatggtattt ataggatata tcatacttag
    16261 aactcaaagt atgcatattt ttaatccaag tggaattaaa agtatggaac aaggactatc
    16321 atttaataca ataataagtt ttatgacaaa tacaaatctt caacattatt caggtgaatc
    16381 aggactttca tactttagtc agatgacagt gataatatat atgatgttta catcagctgc
    16441 aacaggatat gcggctgcta tggcatttgt aagaggtcta gtcggtaaaa agaaaacatt
    16501 gggaaatttt tatgtagatt taattaggat aacaacaaga gtacttttac caggagcttt
    16561 aattattggg cttatattag ttacacaagg agtaccacaa acttttgctg ggacagaaac
    16621 agtgactact atagaaggaa agcttcaaga tatagctaga ggtcctgtgg cagctcttga
    16681 atcaataaaa catttaggaa caaatggtgg tggattcttt ggttcaaatt catcacaccc
    16741 atttgaaaat ccaacaataa tatcaaatat agtagaaata ttatctatga tgatattacc
    16801 aggagcttgt gttgttgcat ttggtcacat gataaagaat aaaaaacaag gttgggttgt
    16861 gtttggcgca atgtcaatca tatttttaat aggtctagta gtgtgcttta aagcagaaag
    16921 tgcaggaaat cctattttat cacaattagg gcttaatcaa agtatgggaa gtatggaagg
    16981 taaggaagtt agatttggta tagcacaatc atcattattc acaacagtga ctacttcctt
    17041 tacgacaggt acagttaata atatgcatga tacactgact cctttaggag gattagtacc
    17101 acttttaaat atgatgctaa atgtagtatt tggtggtaaa ggcgtaggtc tgatgaatat
    17161 gttgatgtac gctataatag cagtattctt atgtggatta atggtaggaa gaacaccaga
    17221 gtttttaact aagaaaatcg aaggaaaaga aatgaagctt attgctttac ttattatatt
    17281 acatccactt ttaatactaa tgttctctgg tctttcagta gcaataccag cagggcttga
    17341 aggaatttca aatcctggat tccatggatt atcacaggta ttgtatgagt ttgcatcttc
    17401 agcagccaat aatggttcag gatttgaagg tctaggagat aacactatgt tttggaatat
    17461 aacaacaggt gtagttatgt tctttggaag atatgtgtct attatagtac tactagcaat
    17521 atcaagtttg ttagcatcta aaaaagctgt aaatgaaagt ataggaacac ttagaacgga
    17581 taattttaca tttacaatag ttttagtact tgttgtttta attgttggtg ctttaacatt
    17641 tttcccagcc ttagcgcttg gacctatttc ggagcatcta gtgttgtggc actaaaaaat
    17701 tgggagggag taaagtttta tggaaactaa aaaaacaaag tttattacaa aagatatttt
    17761 caaaacttcc attatagaag cttttaaaaa attaaatcct aaatacatga tgaaaaatcc
    17821 agttatgttt gttgtagaaa ttggatttat agtaacattg atacttacaa tagtacctag
    17881 tttgtttggt gatgaaggca ataacttaag agcttataat gggattgtaa caataatctt
    17941 atttataaca gtcctttttg caaattttgc tgaagctgta gctgaaggaa gaggtaaagc
    18001 tcaagcagat acacttaaga aaactagaaa agatacagta gcaagggtta tagatacgtt
    18061 aggaaatgaa aaaatgataa gtgcaagtga gctaaaactt ggtgatattg ttttagtaaa
    18121 tgctggagaa gttattccaa atgatggaga agttatagag ggtatagctt ctgtggatga
    18181 gtcagcaata actggagaat ctgcacctgt catgagagaa tcaggtggag atttcgcatc
    18241 tgttacaggt ggtactacag ttgtaagtga ctggttaaag ataagaataa caacaaaacc
    18301 aggagaatcg ttcttagata aaatgatagc tcttgtagaa ggtgcttcaa gacaaaaaac
    18361 gccaaatgag attgctttaa atactttatt agtagggtta acacttatat ttatggttgt
    18421 tttaatatct ctttatccaa tggcaaacta tgcaggtgta aagattccaa tttctactat
    18481 gatagctctt ttggtatgtt taataccaac tactataggt ggattgttgt cagctattgg
    18541 tattgcaggt atggatagag taactagatt taatgtaatt gcaatgtcag gaaaagcagt
    18601 agaagcttgt ggagacgttg atactatgat tttggataag acaggtacga ttacttatgg
    18661 gaatagactt gcatcagaat ttatagcagt tggaaatgca gattcaaaag atttaataaa
    18721 ttattctgtt atgtgttcat taaaagatga tacaccagaa ggaaaatcta tagttgattt
    18781 aggtatgaaa ttaggttcaa caggtaagac aaaagaggca gaggaagctg agtttgtaga
    18841 attttctgca caaactagaa tgagtggtat taacttagct gatggtagag ctataagaaa
    18901 aggtgcttat gatagcataa taaaaagagt aaaagaatct ggtggtacag tgccagaaga
    18961 tttagaagaa aatgtaaatc gagtagctaa actaggagga acacctttag tagtgtgtgt
    19021 agataatgag atatatggaa ttatctactt aaaagataca gtaaaatcag ggcttgtaga
    19081 aagatttgct agacttcgag agataggaat aaagacaata atgtgtacag gggataatcc
    19141 cttgacagca gctacaattg caagagaagc tggagtagat ggatttattg cagaatgtaa
    19201 accagaagat aaaatagatg caataaaaaa agaacaatta gagggtaaaa tagttgcaat
    19261 gacaggtgat ggaacaaatg atgctcctgc acttgctcag gcagatgtag gtcttgcaat
    19321 gaatagtggt acaacatctg caaaagaagc agcaaatatg gtagatttag attcagaccc
    19381 aactaagata ttggaagttg ttgaaatagg aaaacagctt ttaataactc gtggggcttt
    19441 gacaacattt agtatagcaa atgatgttgc aaaatatttt gcgattatac cagctatatt
    19501 tactcttgca atacctcaaa tggagatatt aaatgtaatg aaactttcta cgtcatttag
    19561 tgcgatatta tcagctttaa tatttaatgc tataattatc ccatgtttaa tacctcttgc
    19621 aatgaaggga gtaaaatata gacctatgaa atcacaagct atgttaatga gaaatatgct
    19681 tatatttggt cttggtggag ttatagtacc atttttagga ataaaattaa ttgatttatt
    19741 gattacacct ttagtaatgg ttcttaattt aggttaataa atggaggata taaaaatgaa
    19801 aacattaaag ccagcattac ttgtaagtat tgtattgtta gttgtttgtg gacttgtata
    19861 tccacttgtt ttgacaggtg taagtcaagt tgcatttaaa gataaagcca atggaagtat
    19921 gatagaagta aatggtgtaa aagtaggttc agaacttatt ggtcaaagct ttacagatgc
    19981 gagattcttt aagggaagag tatcatcagt aaactataat acttatacaa aagaagattt
    20041 agtaccagat aaagatggaa atacatctta tggaggtgta tcatcagggt cttttaatta
    20101 tggagctaca aatccagaac ttcatgatag agtacaaaaa gatatagaaa agtttttaaa
    20161 ggataatcca actgtcaaaa gagaggatat accaacagat ttacttacag catcaggttc
    20221 aggacttgac cctaatataa gtccagcctc tgcaaaaatt cagataccag ctatagcaaa
    20281 agcgtctgga atatcagaaa gtaaattaca aaaaattgta gatgataata cttccaaaaa
    20341 actttttgga gttttaggtg aagatagagt taatgtatta aaagttaatg tagaggtcgc
    20401 aaaaatatta ggtcttatat agtattttgt ggaattttag caacaaaata ttacgcaaga
    20461 tttaatttaa aggttaaatt ttaaagagtg aaaatcgata atgattttca cttttttatt
    20521 tgaatattac taatatttgg aataaagtga ttgtaagtat aaaatccaaa aaaaggttgc
    20581 attatcaaaa tgataatact ataatatatt tatcgttatc aatttgataa tagatttgga
    20641 catataggag gaaatagtat gttatataat aacgcattag agttgatagg aaacacacct
    20701 gtagtaaggc tgaataattt aggctgtact aatgggtgtt ctaacatata tgtaaaatta
    20761 gaaaaagtaa atccagcagg aagtataaaa gatagagctg tttatgaaat gttagagggc
    20821 ttagagcaaa gaggagaatt aaaaaaggga gatgtactag ttgaagcaac tagtggaaat
    20881 actggtattg cattatctat gataggaaaa ttaaaaggat atgaagtagt aatcgtaatg
    20941 cccgaaacaa tgagcgttga gagaagagat ttaatgaagg cttatggagc aaatttggtg
    21001 cttacagatg gtaaagctgg aatggcaggt tccatagaaa aagcaaatga acttttaaaa
    21061 gaaaatccaa actataaaag cctaaaacaa tttgaaaata aagataatcc aaatgcacat
    21121 tataaaacaa cagcagttga aattatgaat gatgtcaaag atttagatat attcgtatgt
    21181 ggagttggaa caggtggtac tttaatagga actgcaagat atttaaaaga acaaaatcca
    21241 aatataagag taattgcact tgaaccaaaa aaatctccag ctatatcaga aaacaagact
    21301 ggacctcata aaatacaagg aataggtgct aactttgttc ctgaaaatta tgattctagt
    21361 gtagttgatg aagtaatatt agtagatgat gaagatgcat ttgatacagt taaattactt
    21421 gcagcaaaag agggaatttt agtagggata tcttctggag caaatatatt tggtgctata
    21481 gagatggcaa aaaagtatcc agataaaaag atagttacag tagctccaga tggagttgat
    21541 aagtatatgt ctatgggaat attttaataa ttttatttga gggagtgaat actttgttta
    21601 agaaaataaa taaagatatt gaatatataa tgaagaatga tccagcagct aggtctaaga
    21661 tagaagtttt tttactttat ccatcagttc atgcaatgat aatgcatagg atggctcatg
    21721 ctctttataa gaagaagaag ctttttacag caagattaat atcccagata tccagattta
    21781 tgactggcat agaaatacat cctggtgcta aaatggggga aggtatattg atagaccatg
    21841 gaatgggtgt tgtaattgga gagacagctg aagtagggaa tagggtgaca atatatcaag
    21901 gagctacttt aggagctaca ggaaaagata ctggaaagag acatccaact gttggtgatg
    21961 atgtattaat tggtgctgga acaaaaatac taggaccttt aaatataggt tcaaattcaa
    22021 aaattggtgc aaattctgtt gtcgttaagg atgtacctaa tggtgcaaca gttgttggta
    22081 taccagcaaa aatagtaaaa ataagaaacc tagaacctgt aaaaaagaat aaaaaagaag
    22141 tgtcctatga gtatgatgaa ttagataatg tatattatat ataggattta tataactata
    22201 aaataaactt tataaaaatt attttaaaag gtattttttt caaatttgag gacataataa
    22261 aaaagaactg aatgttacag ttttggttca tcaatctttg ataaacaaga ttttaaattt
    22321 gtagcgtcta ggaggatttt attatgaaag caaatgttaa tcaagatact tgtataggtt
    22381 gtggattatg tccatcaata tgtccagaag tatttgatat gaaggatgat gggaaatcac
    22441 atgttatagt tgatgaagta ccaagtgatg ctgaagaatc agcagcagaa gctagagaaa
    22501 gctgtccagt tgatgcaata gatgtgcatt aataaaataa atgcctataa aaattgaatt
    22561 ttcaattttt ataggcattt gtcttttatt cagttagtat ttcaacacct gtttctgtaa
    22621 ctaaaaccat acattcccat tgagcagata atttaccatc ttttgtataa atagtccaat
    22681 tattttcttc atcaacaaaa atttcgtgag ttccttcatt taccataggt tctattgtga
    22741 atatcatccc aggaaccaat agcatctcag tccctttttt agatacataa cttacaaaag
    22801 ggtcttcatg aaagcttaat ccaactccat gaccacctat ttctctaaca acagagtatc
    22861 catttttctt tgcataagag ttgatagcat cacctatatc tcctaaaaat ccccatggtt
    22921 tggcaacttc aagacctttt tggacacatt cctttgtaac ttgaactaat ttttttgcat
    22981 tttcattaac attacctatc ataaacattc ttgaagcatc tgaatagtat ccattaaaaa
    23041 tagtagaaac atctacattt ataatatctc catcttttaa gataatgtct ttacttggaa
    23101 taccatgaca aacttcatta tttactgatg tacatacact tttaggaaat ccattatagt
    23161 ctagtggagc agggatagct ccattttgta gtgtaaagtc atgaaccaac ttatttatat
    23221 cttctgtaga cattccaatt tcgattttat ctgaaatata atccaaaatt tttgtgttta
    23281 ttacagcact tttttttatt tcctcaattt gactttcatt ttttataata tcatgagttg
    23341 gtacaatatg acctaactta gcaaatgaat ctattttatt atccatatcc atatgacatt
    23401 ttttatattt tttagtactt ccacaccaac agctatcatt tctatttaac ttcataactt
    23461 ttcatccttc ttttattatt tatttaaatt atgggttaca taaactattt taaaacataa
    23521 ccattataaa tattataaca taaatactaa gaaatccttt atagatagtg atttattcaa
    23581 agtataaatt attatatggt tattgttgat gaaaagagga tagttaaatg tattattgga
    23641 tattagcgca cttaatagtt tagttcaagt ttttcaagaa aatcatatgt tagaggacaa
    23701 ttttccttta aagtattttt tgaatcttca tcaagataat aatgtacaaa tgattctgca
    23761 aaaaattctt ctataaactt gcttatatat gcatgtgcaa cttgcttagg gtcataaagc
    23821 ttatatcttt cctgagcaaa tacttccata aattcttcag aatttgattt tttatttaaa
    23881 acatttttat ctatagcatg tgcagtttca tgtaattcta aattttttga tgaatgtaca
    23941 tttgcatagc tagggttact ataacctatt ttagccacta tagaattatt tctatatata
    24001 ccaggtacat ctttccaagt cttgcctgat cttacccaac atggaggtaa ttgatatttt
    24061 aaatcactaa attctggttc atcagttaga tttgaattta ttaattttat ttttatatcc
    24121 ttttctttaa gtgtctgtaa atatttaata tttatttttg caagtctgtt tttcatattt
    24181 tcaacttcat ctttatcata attcaagtca tcaggaagta taagcatttg ttcaattata
    24241 tctatactac tgtcttttat attttcagat tcttcatcaa acttatattg aagagtatta
    24301 ttttcataga tagttatatt tgtattattt tctatagaat aagagtgaat atcctctaaa
    24361 tctaaaatat tcatttgttg taaaatatgt tttgtttcgc cactccatac tactaaagaa
    24421 agtgaattaa aattaattag tggattttct tttgctttta tatcattgct tatatctaca
    24481 tcaaatacat attcaattga gtcttctatg ttaagaccat ctgtattctt tttaatatat
    24541 aaatacgctt gttttaagtc tatataaatc tcatctaaag attcgtcata aaattctgat
    24601 aaattattag taaagagttc ttcacttaaa agttcatttt tatcagttat actttcaatt
    24661 aaaatattaa tttcttttat catatcgtaa atacttattt ttctatttgc tttttcacta
    24721 gatacattgc atggcgcaag agcaatgcaa taaaaatctt tttctttttt ataaaaggct
    24781 ttaagtgatt ttgataaaag tgtaaagtct acacctatct gtaagttgac cttaagtgag
    24841 tcattttgta ataattcagt attatttctt ttagctgtat ttaaattgtt ttccataatc
    24901 attgccccct ttttattagg gtatcataat ttttttatat gtgtcaattt gtttttttga
    24961 aaattataat ttttttgaaa atttta
//
LOCUS       DAMJYX010000049        24587 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJYX010000049 DAMJYX010000000
VERSION     DAMJYX010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.049"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(432..608)
                     /locus_tag="PC479_002097"
     CDS             complement(432..608)
                     /locus_tag="PC479_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804640.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1183..2205
                     /locus_tag="PC479_002098"
     CDS             1183..2205
                     /locus_tag="PC479_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804641.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            2219..3106
                     /locus_tag="PC479_002099"
     CDS             2219..3106
                     /locus_tag="PC479_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804642.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3112..3885
                     /locus_tag="PC479_002100"
     CDS             3112..3885
                     /locus_tag="PC479_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804643.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            3997..4722
                     /locus_tag="PC479_002101"
     CDS             3997..4722
                     /locus_tag="PC479_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804644.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4726..6129
                     /locus_tag="PC479_002102"
     CDS             4726..6129
                     /locus_tag="PC479_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804645.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6140..6964
                     /locus_tag="PC479_002103"
     CDS             6140..6964
                     /locus_tag="PC479_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2804646.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7289..7909)
                     /locus_tag="PC479_002104"
     CDS             complement(7289..7909)
                     /locus_tag="PC479_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2804647.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8175..8507
                     /locus_tag="PC479_002105"
     CDS             8175..8507
                     /locus_tag="PC479_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804648.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8627..9268)
                     /gene="pcp"
                     /locus_tag="PC479_002106"
     CDS             complement(8627..9268)
                     /gene="pcp"
                     /locus_tag="PC479_002106"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2804649.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9289..10221)
                     /locus_tag="PC479_002107"
     CDS             complement(9289..10221)
                     /locus_tag="PC479_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2804650.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10243..10908)
                     /locus_tag="PC479_002108"
     CDS             complement(10243..10908)
                     /locus_tag="PC479_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2804651.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11480..11956)
                     /locus_tag="PC479_002109"
     CDS             complement(11480..11956)
                     /locus_tag="PC479_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HDF2804652.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(11959..12363)
                     /locus_tag="PC479_002110"
     CDS             complement(11959..12363)
                     /locus_tag="PC479_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2804653.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12338..12967)
                     /locus_tag="PC479_002111"
     CDS             complement(12338..12967)
                     /locus_tag="PC479_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2804654.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13333..13376
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13477..14445
                     /locus_tag="PC479_002112"
     CDS             13477..14445
                     /locus_tag="PC479_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2804655.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14447..15010
                     /locus_tag="PC479_002113"
     CDS             14447..15010
                     /locus_tag="PC479_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2804656.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15018..15707
                     /locus_tag="PC479_002114"
     CDS             15018..15707
                     /locus_tag="PC479_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2804657.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15834..16562
                     /locus_tag="PC479_002115"
     CDS             15834..16562
                     /locus_tag="PC479_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2804658.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16576..17250
                     /locus_tag="PC479_002116"
     CDS             16576..17250
                     /locus_tag="PC479_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2804659.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17276..18112
                     /locus_tag="PC479_002117"
     CDS             17276..18112
                     /locus_tag="PC479_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804660.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18128..18799
                     /locus_tag="PC479_002118"
     CDS             18128..18799
                     /locus_tag="PC479_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804661.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18789..20021
                     /locus_tag="PC479_002119"
     CDS             18789..20021
                     /locus_tag="PC479_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804662.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20171..20488
                     /gene="trxA"
                     /locus_tag="PC479_002120"
     CDS             20171..20488
                     /gene="trxA"
                     /locus_tag="PC479_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2804663.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20505..21383
                     /locus_tag="PC479_002121"
     CDS             20505..21383
                     /locus_tag="PC479_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2804664.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21653..22021
                     /locus_tag="PC479_002122"
     CDS             21653..22021
                     /locus_tag="PC479_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2804665.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22024..22395
                     /locus_tag="PC479_002123"
     CDS             22024..22395
                     /locus_tag="PC479_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2804666.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22540..22995)
                     /locus_tag="PC479_002124"
     CDS             complement(22540..22995)
                     /locus_tag="PC479_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804667.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23034..24299)
                     /locus_tag="PC479_002125"
     CDS             complement(23034..24299)
                     /locus_tag="PC479_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2804668.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         9497 a         2730 c         3792 g         8568 t
ORIGIN      
        1 attatttata taaatattga aatttgtcta gttatacgtt tttattgacc agttatgcag
       61 ttgatattga atattctaaa tattggtgtt attttatagg tggtttaaac atattagggg
      121 gattttatat atgatttgta tgatttatag agaatgtata aaatagtaaa ggtttagagc
      181 catgtttagt ggattgcaga atatgcatat agaatgattc ttgtaaatta agttagacat
      241 aggaaatata taatttgtat tggatgatta attttatggt gtatgctttt aacttctata
      301 atgggaaagt aaagatgtac tcccaataca tctttacttt agcagaagtt atagttactt
      361 ataaagtaca aaaaattata tatttattag agtagataaa tctaaaaagg tttttctaga
      421 aaaatttagt tttaattgta tctacttact aataataaga cttgatgtaa gcaggtttca
      481 actaccgttt ctccatattc tgatataaaa tcatctaact gtgtatctaa aaaattacaa
      541 taatcatatg aactactgat gtaagcagtt agtatatgtg tacatttgtc tatttctcca
      601 ataaacatat tatccatccc cctaaaaaat ttagtaaaaa taaaaagtcg cattctatta
      661 gtcaattaac ataatttata aaatttttta gtactagttt ctataactta actccttaag
      721 gttaaatatt cccagtttta ccaatttagt tttattctac aagtatataa tactagaaga
      781 tgctccatta ttcaacatgc ttgtttgaga atagaaaata ttctaaaatt ataattgttt
      841 ttaaaaataa gattcaagat aaataaagtt atatgcaact atttaataat ctttccataa
      901 taaggacagt aggttctatt cttaaaaaac tccatcttta aatttaaaga ataactacaa
      961 tttgaacaac aataaaattt ccaatcaata aaaatatcaa aaataataaa aataattgaa
     1021 aatgtataat ttcccagaaa aataataaat gtaactccaa taataaacac acctataaat
     1081 tataaaataa tttaagcttt atttaaggtt agtattatat ttatataaga tttaagtttt
     1141 aatatatatg tgtaattaaa atgatttagg aggagaaata tattggtaaa ttgggaaata
     1201 aaaaaattgg ctaaatctaa gagtatgttt atatctctag ggatattagt gttaatacta
     1261 atgattagta tctttattac gcctgtttta gaaacagaaa gttattatat agataaagaa
     1321 aaaggacgta tagaagatac gagaagtggt atcgacgtag gtaatgagaa atttcaaaat
     1381 aagattaatg ttttaaagca gttttcaaat caaacagata caggtgaatt ttctaaaaaa
     1441 attgaattaa tgtcaaagaa aaaacttgat aatctaagtg tgaatgaata taaggatatt
     1501 agtttttgga aagtatttaa ctatagagtc acaaattcac ttataaatgt aggaatgtta
     1561 atcattatat ctataattat atccaatatt tatgtagatg aagtcgttac ttcggtaaaa
     1621 gatatcatac tatcatcaaa agagaagaaa aaagcattaa agtctaaaat atttgttgct
     1681 ttattagttc cattagtaat ttattctgtt tatttaatgg gaatatttat tataacttgt
     1741 atgcaacaag gagtacctgt taatggagac ttagaatctt ttagaatagt agacaatgta
     1801 gttgcattga aaactaattt aagtataaca aagtatgtaa tattaaatat aggcatagag
     1861 cttttgatgt ttgaaggatg ggccatgata gccatatttg ggtcttttat aagtaagtct
     1921 tcaatatctt caataagttt ttttatattt attatatcta taactaagat aatgtcggta
     1981 atccatatat tacctaaaaa attactttca gttgtatcaa atgtaaatta ttatgatttg
     2041 atatttggtt ttaataaaat aataggaaat tatctaggtg atattatgat atttaaagtg
     2101 aatatagaca ttgtcaatat agcaatagga cttttgatgg caatattgat aggtgctatg
     2161 tcattatgtt ttttagtaat aaaatcagat tatataagta gataaaaggg gagttaagat
     2221 gaataaatta gaaatagaaa atctttcaaa gatatatggt aaaaaagtag caaacgacaa
     2281 gattacagta actctggaaa atggagttta tggtcttcta ggaccaaatg gagcaggaaa
     2341 atctacatta atgaaacaaa taacaacgtt gataaaacct gatgaaggtc agatacttta
     2401 taatggtgag gatatattta aaatggatgc tgattataga aatattttag gatatttgcc
     2461 acaagaattt ggagtttata aaaattttac agcaaaagaa tttttacagt atattggagc
     2521 tttaaaaggt ttaaggggaa aatatttaaa tagtaagata ggagaattat tagatttagt
     2581 aggtctttat gatgtgagaa ataagtctat aggaaaattt tcaggaggta tgaagaggag
     2641 agttgggata gcacaagttt tactaaacga ccctaagata atagtccttg atgagccaac
     2701 agcaggactt gacccacaag aaagagctag atttaggaat ttaatagcaa aaatgtctag
     2761 agataagata ataatacttt ctactcatat aatttccgat atagaatcag ttgcaaagga
     2821 aacaataatg attaaagatg gcaaactcct aatgaaagga acacacaaag acatattaag
     2881 tcacatggaa aataaagtat ataatgttca agttaaaaat gaatgtgaag ttgatgatat
     2941 acaatctaaa tataaggttg taagtattca aagtgatatt gattcagtag tacttagaat
     3001 tgtttcagag gatattccaa gcgaggataa tatacaacca gttcaagcaa aattcgaaga
     3061 tgtatacatg ttttattttg atttagaaga tgcaagggag gtgtagtaga aatgatgtat
     3121 ttagttaaag ctgaaatgat aaaaatatta aaatcaaaga gtgtttggat aatttggctg
     3181 tttttaatat tttttggatt ttttctgatt agaaagtttg atgttttaga tacttatgca
     3241 gatatatttt acaagataga aggctcaata cctctaatag gtttgataat gtttataata
     3301 acttctggaa attacattaa agaatatgat tctaatatga caggtttaat aaatacaact
     3361 aaaaagggaa aaaaatcagt agtattatca aaactagtag ctaatggatt aattttatct
     3421 attattaata tttcttttat attgttagta ggaattagag gttttagttt ttttgatttt
     3481 aaaaatttaa atgaatcgat tcataactta tggtactttg gaaatagtaa tttaaatctt
     3541 acagtaatac aaatgtatat aattgttata ttgactacta tttttgcttc ttttatattt
     3601 gcacaaatag gcttaacact ttcatctata tttaaatcgg ctgtaattcc atttatacta
     3661 ggtggattga taatggcaat tccatacttt agtgtaggtt ttataccaga taaagctata
     3721 aaatttatgt cagtgactcc aaattggatt atgatgagcc aacaaatggt taagtataat
     3781 gtaccaagta ttttgatagt tttttctata gtaatctcaa ttatattaat gatagtgtta
     3841 actaaaataa catatgaaaa ttttacatca agcaaaaggt tttaactaaa aataatagta
     3901 attttattta tattttagtt taattaaaaa taagataaat gaattttaga taaaatatat
     3961 gctaatatat atgtaaatta ttacaggagg ttgtttatgg aaaatcgtgt tttaataata
     4021 gatgatgaag tagaaatatt gaagctttta gaaactgtcc taaaaaaaga gggccttaat
     4081 aatatatata ctgcaaaaac taaaaaagaa ggattagaat tatttaaaag tattaatcct
     4141 gatttgattg tattggatat tatgttacca gatggcgaag gttatgatat ttgtaaagaa
     4201 ataagaaaaa cctccaattc tccaataata tttttatcag ctaaaaccga agaattagat
     4261 aagttacttg gactagcgat tggaggagat gattatgtta caaaaccttt tagccctaag
     4321 gaagtggctt ttagagttaa agcgcatttg agaagattaa gttattttag tgatgctcaa
     4381 aacgaaagta aaaatctaaa taataatgaa gaaaaaatta tatcatttgg cccatatata
     4441 ttaaatgaaa gtagggcaga acttataaaa gatggaaaat ctataggact gtctgcaaag
     4501 gaactaaaaa tcctatcttt atttgcacat aatcaaaatc aaattataag taaagaaaaa
     4561 ctatgggata aagtgtgggg agaagattat gttggatttg acaatactat aatggttcat
     4621 ataagaaaaa ttagggaaaa attagaggat aatccttcaa agcctgaata tattctgaca
     4681 ataaaaggtc ttggatataa attagcagta aaggaagatt agaatatgaa gtggaagatt
     4741 actagaaatt ttatatttac aattgtgttt gtagctatat cagtagtaat tataaatatc
     4801 atatctatat tgtatgtgat ttcaactaat agttttttta aagttgtaga ttctggaaat
     4861 aatcctgaag aatttgcacg ttcttttgaa aaagatttat atgaaaaaga tggagaattt
     4921 aaactatcta agattggagc tgaaaaatta gaaaaatcaa attcctggat acaggtttta
     4981 aatgatttag gagaggaagt ttatggagta aatgtgccaa aggatactcc caaaaaatat
     5041 acaccctttc aaatggtgaa taattacaag tatatagaaa caaagtatgt taattttgta
     5101 ttagaaaaat atttaaacaa aaaacatcta aacataatag taggtatacc tagtagagat
     5161 atatcaagaa taattttaac ctattctcaa aataacatta aaaaaacttt aaataaagtt
     5221 atcattataa cactagtaat tgatagcgtg gtagctttag gtgtaggata tttatttagt
     5281 agaaaactta caaaaccaat ttcaagcgta ctatggagta ttgagactat ggctaatgga
     5341 aattatagtt tatatcttaa ggacagaggt atatatgaag aagtttttaa aaatataaat
     5401 atgttagctg atactttaag agtaaatgaa gttgagagaa aagaaaatga agagttgaga
     5461 gaggaatggt tggcaaatat aacacatgat ataaagacac ctctagcatc tatacaaggg
     5521 tatgcagaga taattaatga caaagattat gagtttgaag aagatgaaat acaagagtat
     5581 acagaaatta tatataataa gtctaagtat ataaaagatt tagttgatga tttaaattta
     5641 tctactcgat taaaaaataa tactatagtt ctggacaaga aaaaaataaa tttagtcagt
     5701 ttagtcagag atataataat tgatatttta aatgataata gatataaaaa taggaatatt
     5761 gaatttgaaa gtaatgaaga tttaattgaa gtatatgtag attcaatctt attcagaagg
     5821 gctataacca atcttatatt taattcaata gttcataata gcgaaggaac tttgataagt
     5881 gtagaaattg taaaaaaaga taatatagaa attataataa aagatgatgg aattggaata
     5941 agcaaaagtg atttgaaaca tatttttaaa aaatattata gaggtactaa tacaggtgag
     6001 atgcataaag gttcaggtct tggtatggct atttctaaag aaataataga gattcataaa
     6061 ggtaaaattt atgtaagtag tgaaattgga ataggaacaa aaattattat agagataaaa
     6121 caaaattagg agatatgata tggatttttt taaaaaaggt ataagtgggt ttactattaa
     6181 gattttagca ttaatattta tgacatttga ccatatagca gcatttatgc cacaaacaat
     6241 gcaaatacca atttggtttc attgggtagg cagaatatca gcaccactgt ttatattcat
     6301 ggctgttgaa ggattttatc acactagtaa cagaaaaaaa tatataagta gattgtatat
     6361 ttggtctgtt attatggcta taggaaatca aataattaat aatgtatttt cacatccaga
     6421 aggagctata attataaata acactttttc aacattattt ttaatagcga tttatttaca
     6481 agcaatagag ttcataaaga aatttagaaa agagaaagaa attaaatatt ttattatagg
     6541 attattgatg ataatcattc ctataatttt aggaatattt acagtagctt tactttttaa
     6601 agtcactaac agagttattg ctttatttat gattttggta ccagtaccat ttttagtaga
     6661 aggtggacca atttggatta tacttgggat tattttttat ttgtgtagag gtaaaaagtt
     6721 cagtttgagt atttgttatg ttttaatgtg tatatttata tttacaacaa tgtcaaatgg
     6781 agattatagt ttaaaaaatt ctattttaca aaattaccaa tggatgatga ttgcttctct
     6841 acctttaatg ttactttaca atggagaaaa agggaagagt atgaagtatt tattttattt
     6901 atactatcca atccatgtat atatattgta tatcttagga atatacttaa taaatggttt
     6961 ttgattgtaa gtgatatttt taatacaaaa tgaacagtat gaggattgtt ttaggtctat
     7021 taaaattgtt atttagatga gatttgtaga atatttaaat ttatgagcag aaattcagtt
     7081 attgtaattt caataacatt agaatataat attataattt atttagaaat atatgatgtt
     7141 atacatggtt tggttattaa tgtaataata aaacagaggt ttgatataaa attcaatacc
     7201 tctgttttag tgctttttat cctacattta tgcttatttt atttataaat tagtgttttg
     7261 ttttaattaa tattagtaca ttttttattt attttataaa attagtcata attgtttctt
     7321 catattgtgg ttctttaatt ccagcttttt tacctgctga tatacactta agaagccatg
     7381 ccatgttttc tcctagagtt cgcatagtct gtaatccttc ttcatccttc ataacatcct
     7441 ctggagtaaa gccatgtacc tggttccaat actgtgatga tacaataggc atattgctga
     7501 ttgaaaaata tttatttagt tggtcaaatg ttgcacttgc accaccacgt ctacatgaca
     7561 caactgaagc acctaatttt gtagccatgc gactcccagc tatgaaaaat agacggtcaa
     7621 gaaaagaagt taattgacca gttgcactag aaaagtaaac aggagaacca ataataatac
     7681 catcaatatt atccagatct tcaatcaatt cattaacttt atcatttcga atacattttc
     7741 cagtttcaaa acaggatgcg caactaatac atcctgggat tgtttcttta cctaaataca
     7801 gtatttctgt tttaatatcg tgtttactca atactttagt aatctcattt agtgctgtat
     7861 aagtacatcc atattggttt gggctaccgt ttatcaataa aactttcatt ataaatttcc
     7921 tccaatgaat tttatttaat agattttcta agagacatta ttttttataa aataggattt
     7981 tcatgaatat tatcaattta tatagaaatg tctaatctta gaatcctatt taaaataact
     8041 ttttttttag taaatacacc tcctattata aaattgactt tcaattattc ttacactata
     8101 attttattat aaattattct aaaaacaagt acgcacattt ttgttcaata ctaaccaaaa
     8161 ggagagtgta atatatgagt atagactgta tatcaaatac agatttaagt gaaacaggat
     8221 ttagttatac actttcactg attagtggta aatataagat gataattctt tactgtcttg
     8281 tagaatttga agttgtaaga tataatgcat taaaacgtta tataaatact atctcctata
     8341 aaactttgag tttatcatta aaagaattag aagcagataa cttgattatt cgtactgaat
     8401 atcctcaaat acctcctaag gtagaatata gtctatcaga aaggggaaaa tctctgattc
     8461 caattttaga tgcaatgtgt gagtggggag agaggaatag accttaattt tgtatctgta
     8521 gataccataa aaagactgtg agtaaaattg tttgaataat ctactcacag tctatattta
     8581 aattaatagt tagtattttt aatttataaa aatactaaaa aatttattat gaaatttcac
     8641 caccaatttc ttttacatct tctttatata taatacaagc ttctatagct aattctaagc
     8701 cttttactat ttcttctaaa gacatgaatg gtgtattttt tttatctatt acttgtgaag
     8761 ttgtatatgg gatgtgtata aatccacccc ttatattagg atatttcttg tctatcatat
     8821 aaagaattcc atacatcaca tgattgcaaa caaatgttcc tgcagtgttt gaaacactag
     8881 ctggaatttt attttcattc atatgtttta ccatagcttt tacaggcaat tttgaaaaat
     8941 atgcaggttc accatcttct tttataatag tatctataac ctggttaccc tcattatctt
     9001 ttattctaaa atcatctata tttatagcaa ctctctcagg tgtaatgtca aatctacctc
     9061 cagcttgtcc aactgatatt actatatctg gattgtgctc ttgaatagct ttatctatag
     9121 cttcaacaga ttttgtcata actgttggta tagttatctt tatgatttct gcaccgttta
     9181 tattattatt gaccctttca actgcttctt gagctgggtt tattggttct ccgccaaatg
     9241 ggtcaaatcc tgttaacaaa attttcatta ttttacctcc taattatatt aaaatcctaa
     9301 aaagtacatt actaatatgt gggctataat taataccaaa gcaactggag cttgatattt
     9361 tataacaata taatcatttt tacattctag caaggctgct ggaactatat taaaattagc
     9421 agccattgga gtaagtagag ttccacaata tcctgctgtt aatgctaatg ctccaattat
     9481 agcaggattt ccaccttgag acaatacaaa aggaatacca attcctgccg ttatgactga
     9541 aaatgcggca aatgcatttc ccattatcat ggtaaatata accataccta atacataagc
     9601 tataacacca aataatctgc tatcagcagg gactacacca gatatcatgg ttgaaataac
     9661 ttcaccgact ccggcttgag taaataaagc tcccaatgca actaaaagtt gtggtaacat
     9721 actagcaggt cccatttgtt gaagtaatct tgagccatct tgaactgatg tagcaggttt
     9781 tgccttagtt attataaggg ctgatattaa tgcaattatt gaacccacac ctattgcaaa
     9841 ctgagaagaa ttatcaaaat tagaaagact catagctact actactgcag caaatgctaa
     9901 tattaatgat ggtataaata ttttattttt aagtttgtct gcttgttctt ttccaaaatt
     9961 ttcatctggt tcttcatact ttgcaaatac aacttgttta gaaagtgtta ataaactaga
    10021 taatattaat attattccta ttatattggc tggaatcaat ctaccaaata tgaatggcag
    10081 tgatataagt atccaaaata atgatgtacc atatttctta ttgttattta tggtagtaaa
    10141 agacttatat gctataacaa tcattaaaaa tcccattagt gcgtaaaaga tttccaatga
    10201 tatgtttata aaattttgca tttttattcc tcctaaataa aactattttt tatgactttt
    10261 gtttaatttt ttatcatata aggtaaactg gattgtagcc actacaagtg ctattactgc
    10321 cataatagta ctagccttta cgatattaag tccagttaca ttgtatccaa attgagtcag
    10381 agtagatgct ataagtaata ctccactact acctgcaaat aagttttgac cataaaaatt
    10441 tccatagttt tctgatgcag cagataatgc ctttatggct tcttcatccg attcttttag
    10501 ttcatcacta ttacttaaac cagcagcttg tgccatagga ttaacaagtg gtcttacaaa
    10561 ttgaggatgt ccactcattc ttattgatag agcaccagct atttgtctag ctatcatgta
    10621 tatagtaagt acttttcctg ttgtaagtct ttttaatttt tctattaaag aaacagctct
    10681 ttgtcttaaa ccatatcttt ctaatacacc gattactggc aatgttaata tgaataatga
    10741 tacacctctg tttgagacaa aagaatctcc taaaattgta aaaatttgat taaaatctaa
    10801 tcctgcaaca attcctgttg ccactcctgc taataataca gtaaatagtg tgtcaatttt
    10861 aagtataaat ccaattacaa ctatcaatat accgattagt tttatcataa attgttctcc
    10921 ttccatttct atatgtttta atttaaattt atttaatgga taaagttaaa ctctttttat
    10981 agtatttgcg ttaataggac tattattata aaatttaata aaaaataggg aagatgtttt
    11041 aaagtatcaa agtaaataat ggttgtgtat aggaatttga ataacaaaga ttatatttta
    11101 agctaatatg aattgatttt gtaagtctat agtttaagta tatgtcaata tttgaaatag
    11161 tagttttaaa ttaaacatta tattataaaa agagaatgtg ccaaacatag agacaagtgt
    11221 atttttctct gttgaaatga taaaaatata tttgatttca tacaaaaaga gagtgtctaa
    11281 tgaatttttt agttcattag acactctctt tttgttatga ttaataaaac atatttgtta
    11341 tgattaataa agcatatatt aagtgctagt ttcattagat ttcactaaga aaaataaaat
    11401 tttgataaag tttaaagact attttgagca atattactta taaataaaag aatttatcat
    11461 agaaataatt agctttattt tagctattaa gatttattaa aaaattatct atattatcct
    11521 gtgatattct aaaactgtca taattacctt ttgaaacagc cttcataata agtttatcta
    11581 aaaatttcat tttttctaat cgagcttcac taccaaaaat tccaatagca tttgcacttt
    11641 ctaataagga tgatggaaaa tttgatttta aatatttatc tgcttgttca gaaaagccac
    11701 agcaaaggaa aatgccaact tgttttttgt ttaataactc tacattatca ttacatagca
    11761 cctgtatttt ttttgaaact gaaccaacat atatagaact tccaataata attttatcaa
    11821 attttgataa ctcaatttta ttattattat tattaatatc aacaatagtg acattatctg
    11881 atagtttatt ttttagtata tttgcacagt cttttgtaca accatattta cttgaatata
    11941 taataatagt attcataatt attctccctt tcttaaagca ttttctacag cttttcgtat
    12001 agtattactg ctataagttg cagaagttat agcatccaca tctaaagact gttgtttaat
    12061 aatacttgtt gtaatttttt cagcaggttt acctaaaaga tggtcgtgtt ttaagatatt
    12121 aatattttgg attttaccat tattaacttc aacagacaca gtagctttta ccaagttatt
    12181 atcagcactt ccagtataaa tgccattttt aacattatta atatctatat cttttactac
    12241 caagttttct ggctttaagt tgcttacaat tacaataata atgcatataa atacaaagaa
    12301 tagagcaata gatattttta catatttttt ctttttatta gtagccttgt tgcgaatttt
    12361 cattgtaaag tcctcccact aaaatatcta agaaattttc tataagttga gaaaaattat
    12421 gtaaagaata tagtttattt tcaacaacac ataaaacgaa tcctaacaat gcacttataa
    12481 gcatccatga gcttttatct atattttttt tgaatattcc ttgttcaatt ccaatggaaa
    12541 ggaatttatc aagcattttt ataccattat tgctaggcgt attgtttgta ttttcagatg
    12601 caaatataat attcattcta ctatgcatga tagattttgc catctctggt tcactagaaa
    12661 gagtttttat aaagctagtc ataatttcta tgatttgttt atctattggt agtgatgagc
    12721 aattattcat aatgtcaatg gcattacttt ctactgtgtt ataaagttta ttggtcatat
    12781 cgctgataat ctctgctttg tctttgtagt aaagataaat tgtagttgga gaatattcta
    12841 tttttgtagc aatttttctt attgataaat tttcatatcc atgttggttt ataatttcga
    12901 tgctagcatc cataatcttt ttcttcattt cttctttttc atttttacgt ctttctttga
    12961 tactcatata atcacctctt tgcttaacgg tgttaagtta atttacactg ttattgtata
    13021 atttaatttt aatactgtca ataaattatt aaataatata agttaatata tttttatgga
    13081 taataaatta atgttattaa ttgtaatatt taagatatta ttgtataata ataatcggtt
    13141 taaatgcgcc acatcttatt aaagttggtt cataattcta aataaacttt aataagatac
    13201 attaaaatat aataaattct tttatgctat aaaatttatt ttatagctat taaatcagat
    13261 gtttaaaaat tttttaacgg tctatagttt ttaagagtct tatagtaagt agcctaaaaa
    13321 tatgatttta catctgtact tgttcgagaa ctccaagtat aaaaaactaa ggaattatat
    13381 agtggcagta tataattaat acccttagac acaccttttt gaaagtggaa aaaagatgtg
    13441 tagttctcct cgaccacaaa tcaaggagga taaaaaatgg aaaagagaaa agtaataatt
    13501 gattgtgacc caggaattga tgattctttg gcaattcttc tggctttaaa ctcaccagag
    13561 ctagaagtaa ttggaattac cacatgttgt ggaaatgtcc cagcaaatat aggtgcagaa
    13621 aatgcactaa aaacacttca aatgtgttct tcactaaata ttccagtata tataggagaa
    13681 gaagcaccac taaaaagaaa acttgtaaca gctcaagata cacatggaga agatggtatt
    13741 ggagaaaact tttatcaaaa ggttgtagga gctaaagcaa aaaatggagc agtggatttt
    13801 ataataaata ctttatataa tcatgaaaaa gtatcaataa tagcacttgc accacttaca
    13861 aatatagcta aagcacttat taaagataag aaagcatttg aaaatctcga tgagtttgta
    13921 tctatgggag gagcatttag gattcatgga aattgctctc cagtagcaga gtttaattat
    13981 tgggtagacc cacatggagc agattatgtt tacaagaatt tatctaaaaa aatccacatg
    14041 gtaggtttag atgtaactag aaaaattgta cttactccta atattattga gtttataaat
    14101 agacttgata agaagatggc aaagtatata actgaaataa ctagatttta tattgatttc
    14161 cattgggaac aggagggaat aattggctgt gtgataaatg accctctagc agtagcatac
    14221 tttatagata gaagtatatg taaaggattt gaatcatatg tagaagttgt agaagatgga
    14281 atagctatgg gtcagtctat agtggattct ttcaatttct ataaaaaaaa tcctaatgca
    14341 attgttctaa atgaagttga tgagaagaaa tttatgtaca tgtttttaaa gaggcttttt
    14401 aaaggttatg aagacattat agactctgtg gaaggagtga tatagtgtga aaagaaaaat
    14461 atgtgtaaaa actattacac ttatagcttt tggtatttct ataaatataa taggcgcttt
    14521 tatagctatg ggattaagat tacctgtata cttagattca ataggaacta taatgatagc
    14581 ttcactttta ggacctaaat acgctgttgt tacaggtgtg tttggaagtt taataagtgg
    14641 aattacgttt gatgtgtatt ctctctattt tgcaccagtt caaatatcaa ccggattact
    14701 tgctggaata gcatttaaaa agaatttttt aaggggttta aaaactccac taggagtact
    14761 tttatttgcc attccaacat ctataataag ttcaataatt tcagcatttt tatttggagg
    14821 tatgacctct tcaggttcat catatatagt gcaaatcttg aaagtgttag gactggggga
    14881 tgtttttagt gtgtttgtaa cccaagtatt tactgattat ggagataagc tgttagcagt
    14941 tgttttagtg aatttaggat taaatgcagt tccaaaaact ttaaaggtat cattgactgg
    15001 aggaaaatag atagaaaatg gatagatata gtgagataac aaataaaaat caaagagaaa
    15061 ttgttctgct taaaggattt ccttgtatat ggggaaagtg ttctttttgt gattatatag
    15121 atgataattc taatttagaa gaagagatga ataaattaaa tctaaaggtt ttaaaaaacg
    15181 taactgggaa atatggagtt cttgaagtca taaattctgg aagctgtttt gaattaccta
    15241 aggatacttt agaaaaaata aagtgtatta taaaagagaa gaatataaaa aaattatttt
    15301 tagaaagtca ttggtcttac aaaaataggc ttaaggaaat gagagagtat tttgaaattc
    15361 ctgtggtatt taaaattgga gttgaaacct ttgacaatga ttttagaaat aatattttaa
    15421 ataaaaatgc aaattttaag actccacaag atgtaaaaga atattttgat tcaccttgta
    15481 ttatggttgg aataaaagga caaacaaaag agatgataga taaagatata gagattatcc
    15541 taaatacttt tgaaaaagcg acagtgaatg tttttataaa taattcttct agtataaaaa
    15601 gagatgaaga gttagtgaaa tggtttgtta gtaagtataa attcttggat gaaaatccaa
    15661 atatagaagt tttatacaat aatacagact ttggtgtagg agattagcat taatttagtt
    15721 taaatttaaa attgaaaagt ttcttatgtt tttgaaaaag cttctaatag ttagaagctt
    15781 tttttgtaga gttttatgtt acaataagaa aaaaggaatt aaggagcaat taaatgatgt
    15841 ttgatagggg aaaatggaat aaattggatt ataatgaact tttagaatat atgatgtcta
    15901 tacaagatat aaaatataga gattttaata aaaagcttat acctggaaca gaaaatataa
    15961 taggtattag agttcctaat ttaagaaagt tatcaaaaga aataagccaa gggaattgga
    16021 aggaattttt agaagtagca caggatacat actatgaaga agttaggcta caaggtatgg
    16081 taattggcaa tataaatagt aattttgaag aaactttata ctatgtaaaa agatttatac
    16141 ctaaaatcga taattggtca gtttgcgatg gattttgttc tgatttaaag tctatgaaaa
    16201 aatataaaga aaatatgtat gatatactta aaaaatatgt ttattcaaag aatccatggg
    16261 aaattagatt tgcattagtt atgtttttaa tatattatgt tgatgataaa catattaaag
    16321 aaatatttga atactgtaat aatatacaaa gtgaagaata ttatgttaaa atgggtatgg
    16381 catggttgct atcaatttgt tttattaaat gtgaagagga aacttttctc tatataaaaa
    16441 ataataattt agatgatttc acttataata aaatgctaca aaaaataatt gaatcaaata
    16501 gagtagattt agaaaagaag aatataataa gaagtatgaa aagaaagaca agaagatgtt
    16561 gacggaggaa tattaatgaa taacaatgaa gacagaattg acaaattttt agaacttcaa
    16621 agcaaaggat ttaaatttgc agagatagct caaaatctaa agattagtca aagtacttta
    16681 agaacttttt taaataaaaa aggatataag tcagaaaaag gtaaatacat attaaaagac
    16741 gaattaaatt ataataaaag tttggataat agaaacttgg aagaagagaa agctaaggag
    16801 tatgaaaaaa gtaaaaagaa tactataagt aatagaaaaa aagtaagcac caaaaaaaag
    16861 atttctaatg aaaaggcttt aagtaaaagt gttgaacaaa ttgagtttaa tgatacaggt
    16921 ttaaaaaatg caaagccaaa acaaaatatt aagcctaaga aagatagaaa aataaatata
    16981 actcaagaag atttagataa gctttgtgaa gtatatgatt ggtatttaga agtaaaagat
    17041 tacaatataa agcctaaaaa agtatctaat aaaaaagatg tacatataga tgatgaacac
    17101 atagatgaat taaaaacaac aagaatacgt atagataaag aaatatggaa tgattttgaa
    17161 agattatgta gtaattcgaa ttttgaaaaa catcaaatac ttacacaggc attgaaagaa
    17221 tttatgaaaa agtataaaaa tctactataa aatactacta tatgaggtgc ttaatatgaa
    17281 taaaatagta aaaaaatcaa tttctctaat gattatatta acaatattta tttttatgtt
    17341 gacagcatgt gaaaaagatg aacagcctga ttcagttgat attcaaacag aagataaaaa
    17401 tgagataaaa atagatgagg gaaatgcaaa agtattgaat ataggaaaat cagaaattat
    17461 aaatatagat gaaggtgata aagtagatac atcaacaaag gtagaaaata attcaacatt
    17521 taatatatcc aatgttgaat tgatatataa tgaatatgat gagaataaaa aaattatatc
    17581 ttcggatagt aaggctttgt tggacatgac tttaatgcct gggaaagttg cttacataga
    17641 atgtgggcat aaaacttttg taaagggtgt agaagtttat gcatatgaat atgaggcaga
    17701 gggtaagatt gtgtatgtta atttaaaaga aaatactatt aatataagaa ataataatat
    17761 taagctagaa aatagtagtc agtatgaggt gctatctaca tcagaactta aaaaagtaaa
    17821 tgaatcaaat gagggaatta cgtatcaagt aaaagtaaaa aattcatcat caaaagattt
    17881 aggaaatatt attcttaaga cagcagaagt taatgaaaat ggagaatatt taacagtaaa
    17941 tcgtgtacca tcatataaga ttttaaagcc ttcagaagag acagatatag atatattgtg
    18001 ttcaacaaag gcaaaaagtg ttgaaatagt gggatatacg tatgatgata taaaagaaaa
    18061 agccaatgtg gatattgact taaaatctca taaagttaag atagacaaat aaaggtaggg
    18121 gatacttatg aaggttttaa tagttgaaga taataagata cttttagaaa gtgtagtaga
    18181 agaacttagc aagcattttg aaactgaaaa gtgtgaagat ggagaagaag ctttatactt
    18241 aataaatcaa aatatatatg atttagtcat tttagactta atgcttccta atataaatgg
    18301 atttgatatc ctaaagaaaa tgagaattaa taatatagat acacctgtac ttatattgac
    18361 agcaaaagaa acattagatg acaaagtaga agcatttact ataggagcta atgattattt
    18421 gacaaaacct ttttatatgg aagagttagt agctagggta tacgctatat taaggactaa
    18481 tggaaagata aaagaaagaa acggattaga atttaaatct ttatatttag atacactaga
    18541 aaaaagagtg tatattgaaa aagaagaaat aaaacttcaa aacaaacagt ttaacttgct
    18601 tgagtatttt gtattaaata aaggttcaat tttattaaaa gagcagattt atgatagaat
    18661 atggggaatt gattcagatg caactattga aatagtggaa gtatatgtaa gtaatctaag
    18721 aaaaaagcta agtaaatatg gatatgataa atatattaaa actaaacgta aggtaggata
    18781 tatattcgat gataaataag aatgtattta ccagtactaa aaatcattta ataaaaatgt
    18841 atattattgt agtaggtagt tttttaataa tcttttctat atttatatac tcatatttta
    18901 ggggacttac atatagtgga attgatagtg agataaatga tgaacttgaa tatatagtct
    18961 ctcaatttaa aagaacttca tttttgaacc caataagact caaagatcct aaagatatgg
    19021 tgtatgtata tgaagatgga aggataagtt actatactca aaatgaatat tttgatgagt
    19081 tacttccaga tagaagacta gataaaaaaa attcgttttt taaatacaca gaaaatggat
    19141 atacttttag agaattaaat gtagatgtag gtagatacca aatacagata attagaaata
    19201 tagattctga gatgaattct ttaagacagc ttacatctgt tcttataatt ggaatattaa
    19261 tttctgtaat aattacttac tttgtggctg tatatctcac tagaaaagct ttaattccaa
    19321 tagaaacagc atggaaaaat caagctaagt ttatacaaga tgcttctcat gaattaagaa
    19381 caccaataac aatagtttca tcaaaattag aaagtatgtt aaaatcccca gaaagtacag
    19441 taaatgatga agttgaaaca atagctactg caatgaagga aactagaaga cttaaaaaaa
    19501 tgataactga tttattgtct cttactaaag aggactctat tgttaaagtt aatttagaag
    19561 aaatagattt agaaaagtta ttggaagaaa tatcagaaga ctatattgac attgctgagt
    19621 ttcaggaaaa aagattcgtg tttaattcta aactgaagaa taaagttata ataacagata
    19681 aaaataaatt aagacaactt atattaattt tcattgataa tgcctttaag tatacaaaat
    19741 tgggtgatga gatagcatta gaattgaaag aagatataga ggatgaggta actttactta
    19801 taagtgatac tggtataggc ataaaaaaag aagagatacc tcttatattt gatagatttt
    19861 ttagaagtga aaatgtaaga aataaagatt tagaaggttc tggaatagga ctttcaatag
    19921 ctcgcatgat tagtttaaac ttatctattg atataaatgt aacttctgat gtagatattg
    19981 gaactacatt tgaattgtca ataccaaaaa agttaaaata ataaaaatat aaaaaattgt
    20041 ttttagaaat gaagaatggg tatactaatt agtataaaat ataaaaagac cttataaaag
    20101 taaatttata tttataaatt tcatagtata attatatagg gataataaag tttaatagga
    20161 ggatttaaaa atggcaaaag ttataaatac atctgaattt agaagtagtg ttgaaggtag
    20221 taagggagta gttgtagttg atttctttgc aacttggtgt ggaccttgta acatgttggg
    20281 acctgtattt gcagaattag gtgaagagat gaaggataaa gctagatttg taaaagttga
    20341 tatagatgag agtttagaaa tagctcaaca attcaatgta tctacagtac caactatgat
    20401 tatatttaaa gatggaaaac cagtagaaac tttaattgga ttcatgccaa agaacaaaat
    20461 tgaaatgcaa gtaaaatcat atttataaaa aatgggtgag tattatgaga tatgatatag
    20521 ctataatcgg cagtggtcca gctggattgt ctgctgctat aaatgcaaaa ataagaaata
    20581 aaacaattat catgtttggt aatgataatt taagtaataa gcttgttaaa gctccttcaa
    20641 tagataatta tttaggtttt tatgatataa gtggtgatga attaaaagac aagtttaaat
    20701 cacatataga tagtatggat atttcaattg aaaataaaag aataaataat atttatgcta
    20761 tgggagaata ctttacaata atgtctggaa atgatatgta tgaagctact actgtaatcc
    20821 ttgcaactgg ggtagagtat actagaccaa tcaagggtga agaagaattt ctaggaagag
    20881 gtgtgggcta ctgtgctaca tgtgatgcac cactttatag aaataaaaag gtagcagtta
    20941 taggatataa tgaagaatct aaagaagaag caaatttttt aagtgaactc acttcaaaga
    21001 cttattttat acctatgtac aaaaaagata atcttatgag aagttcagat aacctagatg
    21061 attctattga agttatacat gacagaccag tacagataga tggagataaa cttgtaaaca
    21121 aagtatcttt taaagaaaac cacattgaag ttgatggagt attcgttata aaagatagta
    21181 cggctccaag tgcattggtt cctggaatag agattgatgg cattcatatt aaagttgaca
    21241 ataatatgaa gactagtata gatggatgct ttgctgctgg agattgtgtt ggaaaaccct
    21301 attcttatat aaaagcagct ggtcaagggc aaatagcagc tttaaatgct gtttactatt
    21361 tagataaatt aaagagggct taatatttat attgattaac tttttaaaga tatttataaa
    21421 aaactgtctt gaaataattt tattatttca agacagtttt ttatttttaa gttattaata
    21481 aaagtatttt aaaatctata tgtaattact tttattaata aaactcaaag ttatattagt
    21541 aaaaattgga attatttata atatactcca aaatttaaaa ataatggttg aaaaatctaa
    21601 ttaagagtac tataatttaa ttaagtaatt acttaatcaa aatgaggtac ttatggaaaa
    21661 cttggttaaa atatttaaag cattgagtga tgaaactaga cttaacatac ttatattagt
    21721 atccaagaga aatatatgtc aaaaggggat ttctaaatat ctagggattt cagattctgc
    21781 tgtttcacaa catataaaaa tattaaaaga tgttgggata ataacaggat ataaagaagg
    21841 atactatgtt ttatatcata ttaataaaga atcatttaat atatgtgtag attttataaa
    21901 ttctatgtta agtaattcta gtgaaagctt tatagatgta tttgatgtaa atacaataca
    21961 tctaggatgc agtaaagaat gtaaatctat aaaaaaatgt tgtaagagaa gggagaaata
    22021 ataatgaaag tatgtatacc agtagaggag aataaaggat tggatagtaa accatatgga
    22081 cattttggtt ctgctccaat ttttgttgtg tgtgatttag aaagtggaga agttaaatct
    22141 ttagataatg gagatttaga tcatgaacat ggaaaatgtc aaccattaaa agcattgtca
    22201 gggacagctg tagatgcagt agttgttgga ggaataggac aaggagctat aattaaatta
    22261 aatggaatgg gaataaaagt ttacagagca gaaggtgata ctataagtgc taatttagac
    22321 ttacttaaaa atggaaaatt agtagaattt ccatcagacc atacttgctc acatgacgga
    22381 tgtggacatc attaaaaaaa aacagctgat gaaatatata cttcatcagc tattttttct
    22441 tttaataaat acgattttat tcttttaaaa ccaaaattta gatttttatg ttttttgtag
    22501 tatccttaat aatatttcaa gtttacaagt ttactacttt tagatttttt ttgtatgttt
    22561 ttctatatag tcaatatcat ttaatatgtc attgagttct ttattaagtg tttttaaatc
    22621 atcaccatct acattgtagt ttaaaaatag ttcctctaca ttatcaatga tagaattgac
    22681 ttttctagta acttcaacaa atgtcttaaa gttactacta tttttagcat ttagaaattt
    22741 ttttaaatcc tttgatataa atgatactat ttcatattca tcattaaata gacctctacc
    22801 aataggatat ggttcaattc tctgtaaaaa atattggtct ttagcattaa tattataagt
    22861 ataagatatt tcccaaccgt ctcctaaatc aaacttgata aaatataatg tactacttac
    22921 ttttgatatt cttgtggcac caagctgtct taaaagaact tcaatcgaat caccttgaac
    22981 agtattaata ttcatatata cacatcccct ttagtattat tatactatat tttttaaaat
    23041 tttataatta aataaacagt agatataata atagatataa tcataagtgg aaaacctatt
    23101 ttgaaatact ccttaaaaga gataggatga ccgtgtttat tacctatacc tgctagtaca
    23161 acattagcag atgctcctat tagtgttcca tttcctccaa gacaagctcc aagagatgtt
    23221 gcccaccata gtggcattac atcaatacct tcagcttgca tagtcaaaat aagaggaatt
    23281 agtgttgcaa caaatggaat attatctaag aaagaagaga ctatagctga caaccataat
    23341 atcaacaaca ttgtaaatac aagatgtcct tctgtaagtc caatcaatgc ttgagccaat
    23401 ttgtttatta tcccaacttc tactaatcca ccaactacta caaataatcc cataaagaat
    23461 aaaattgtag accattcaat acctgccata atttcatcta catcttgttt tcctattaaa
    23521 agcattatag atgctcctgt cagtgctaca acagaagaat ctatgtggat tgtactgtga
    23581 aacataaaac caaaaagtat gaatgctatt aatataaggc tttttataag tagaggttta
    23641 tctttaacag attttttttc atctagtttt aatattgcat ttttagctct ttcattcaca
    23701 acaagttctt ttccataaat aaatctaaag catattatag ttatagcaag tattactata
    23761 acagcaggac ctaaatttat tacaaaatcc ataaaactaa gattggcagc actaccaatc
    23821 ataatatttg gagggtctcc tataagagta gctgttcctc ctatatttga agctaaaatc
    23881 tgtgttatta aaaatggaac tggattaagt cctaatattt gtgtaataac tatagtcatt
    23941 ggtccaataa gtaagacggt tgtaacatta tctaatacag ctgataaaat agctgttata
    24001 atagcaaaac aaatcattat cttccatggg tcacctttgg ctttctttgc agtccagata
    24061 gctatatatt caaataaccc cgagtttttg acaacagcta caaatagcat cataccaact
    24121 aatacaccaa tagtattgaa atctatatgg cttaatcctt catcaagtgt aagtatattg
    24181 aatattatca ttaatagtgc accaccaaca gctgctacgg atctgtttac cttttctgta
    24241 ataatagctg ccataactat taaaaatata catatagcta ttatttgttg aaattccata
    24301 atataagtac ctccatttgt attattttgt gcctaatgct agtactatat atagtattac
    24361 ttttttattg ttttttcaat ggatgtatga aagtatatta tttaacaaag tttttggaaa
    24421 attgacagaa tttaaagaaa atttttgtta tttaaataaa ttttaaaatt attccattga
    24481 taataatatt aaacgacaaa cgaaaaaata taccctttaa actagaattt attgtatcta
    24541 ataacaatta taaaatatta aatatatcta tattataaaa acaataa
//
LOCUS       DAMJYX010000050        24275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJYX010000050 DAMJYX010000000
VERSION     DAMJYX010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.050"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..1008)
                     /locus_tag="PC479_002126"
     CDS             complement(<1..1008)
                     /locus_tag="PC479_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2804669.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NL"
     gene            complement(1056..1916)
                     /locus_tag="PC479_002127"
     CDS             complement(1056..1916)
                     /locus_tag="PC479_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2804670.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2185..2394
                     /locus_tag="PC479_002128"
     CDS             2185..2394
                     /locus_tag="PC479_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2804671.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2394..2660
                     /locus_tag="PC479_002129"
     CDS             2394..2660
                     /locus_tag="PC479_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2804672.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2689..3261
                     /locus_tag="PC479_002130"
     CDS             2689..3261
                     /locus_tag="PC479_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2804673.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3496..4686)
                     /locus_tag="PC479_002131"
     CDS             complement(3496..4686)
                     /locus_tag="PC479_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2804674.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4946..5377)
                     /gene="dut"
                     /locus_tag="PC479_002132"
     CDS             complement(4946..5377)
                     /gene="dut"
                     /locus_tag="PC479_002132"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2804675.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5490..7718
                     /locus_tag="PC479_002133"
     CDS             5490..7718
                     /locus_tag="PC479_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2804676.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7829..8314)
                     /locus_tag="PC479_002134"
     CDS             complement(7829..8314)
                     /locus_tag="PC479_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804677.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8566..9789
                     /locus_tag="PC479_002135"
     CDS             8566..9789
                     /locus_tag="PC479_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2804678.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9793..12927
                     /locus_tag="PC479_002136"
     CDS             9793..12927
                     /locus_tag="PC479_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2804679.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12961..14259
                     /locus_tag="PC479_002137"
     CDS             12961..14259
                     /locus_tag="PC479_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2804680.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(14548..14793)
                     /locus_tag="PC479_002138"
     CDS             complement(14548..14793)
                     /locus_tag="PC479_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2804681.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(15007..17634)
                     /locus_tag="PC479_002139"
     CDS             complement(15007..17634)
                     /locus_tag="PC479_002139"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2804682.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(17695..18537)
                     /locus_tag="PC479_002140"
     CDS             complement(17695..18537)
                     /locus_tag="PC479_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2804683.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(18544..19182)
                     /locus_tag="PC479_002141"
     CDS             complement(18544..19182)
                     /locus_tag="PC479_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804684.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            19455..20066
                     /locus_tag="PC479_002142"
     CDS             19455..20066
                     /locus_tag="PC479_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2804685.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            20364..20702
                     /locus_tag="PC479_002143"
     CDS             20364..20702
                     /locus_tag="PC479_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2804686.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(21109..22545)
                     /gene="miaB"
                     /locus_tag="PC479_002144"
     CDS             complement(21109..22545)
                     /gene="miaB"
                     /locus_tag="PC479_002144"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2804687.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(22793..24229)
                     /locus_tag="PC479_002145"
     CDS             complement(22793..24229)
                     /locus_tag="PC479_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2804688.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         8408 a         3832 c         3217 g         8818 t
ORIGIN      
        1 taggttatta catttattta tatatatttt ttcattatct aagtcttcat caaacatttt
       61 tattatattt tttatctcat acttgtactc atcttcattc attctataaa gcattaaatc
      121 ttctagtaca tctttcatgt cgaagtacac atcttctaat aaataaaaat agccttcctc
      181 tatatctttc aatttatcta tagtatccat ttttttactt atatctttca taatttctaa
      241 attttgtata tttacatcta tgattgttaa taagtacatt ctcacagttt ttattatttc
      301 aacagaaaaa tctacaatat tataatttcc ttctttttta tataatgact ccttaaattt
      361 gttaaaacaa ttatctaaat tagaatttat atcgttaaat ggtggtctta ttatgtcacg
      421 tattattaaa agattttgta tatctacatt atttgtgatt gcctcatcca aaataaataa
      481 attatatgca tcatttaatg ggtcatgggc atctccacta aactctaagt taacaaattc
      541 aagtaaattt ttcaatgata tatagttcat acatctcata ccacaattta tatatttttc
      601 ttttatatct acaaataaat ctctaatgtt atttacaaac cttggatggt tatttttttt
      661 agcacttctc atatctgtgt tattgaaaca tgtcaaatcc aaattaccaa aagtatatat
      721 tcctttaata tcagaaaata tgccaagcca ttttttaaac tttctcatga cctcttcata
      781 acttggtgca ctcttcaatt cttcagaaga tatatgtgtt aattcttgta tatgaggata
      841 caactcctca ttacttactg gtcttattaa agatttaaat ttatcaatat tttttgtttt
      901 ttcatcatac acaactgctc caattgcaat tatatcagaa ttaaatactg ctctttttga
      961 tttagaattt ggcatattca tttcaaaatc tatatatact cttttcaata taaacaccct
     1021 ttccaattgt ttatagtgat tctttttaac tatttttatt caaaattttc taagaaagaa
     1081 tgtttctccc catctttttg ccaaccaagt actgaatcaa gatttacatt ttctgccgcc
     1141 ttaaatatag atttttctac atttttaaaa tttgattcta aactctttat taagtctttt
     1201 atctcatatc ttttattacc tgttttttta gcagcaacca tacatgcaca atttaatggc
     1261 catataccac tattttgaat aaatcttata atatgttctt ctcttatata ataaagtggt
     1321 cttataattt ttataccttc aaaattagta gaattaagtt taggaagcat tgttttaaaa
     1381 tttccagcac acaataagtt aagcattgtt gtctctatta catcgtcata atgatgacct
     1441 aatgcaagtt tgttgcaccc tagttcttct gcctttgaat ataatgctcc tctcctcatt
     1501 cttgcacaca tatagcatgg ataatctttt gctatttcat ctgctatctc aaatatcctt
     1561 gaatcaaata aatgtattgg tatatttaga tactcacaat tatctataag taattgtctt
     1621 atatttgcat gatatccagg gtccatagca ataaattcaa catcaaaatt aacttgtcca
     1681 tgttttttta actcttgaaa cattttagcc ataagaatac tgtcttttcc accagaaatc
     1741 gctacagcta tcttatcacc ttcttcaacc aatttataat ctcttattgc cttcataaac
     1801 ttagaccata aattttttct atattttttt attatacttt tttctatatc tcttactgga
     1861 agtctttcct caaaaggaac tataatttca cagccttttc ctgatagttc actcatctaa
     1921 tcacctcgta tatttatgtt aataatattt tacaaaaaag tctaccttca tccacaaaat
     1981 actataattt ttactttaat tttactaaat aatttatagt ttacaataac acataaactt
     2041 ataataacat aaataacctc tttcttttat tatctttttt atttttctca tttaatattt
     2101 tattattagt aacgattttt aaatgattga atattataca ctagatagag ttacctatct
     2161 atttacaaca aggaggtttt catcttggaa aataagacta gagatatcaa aaaaaattgt
     2221 ggtccatctt tggctagacc atacattaat aatcaaattt tcacagaaat gtactgcctt
     2281 tctgatgcat taaaatttgg aactatattt ccagaactaa acttactaga gtctgaaatg
     2341 tacaacaaag aattatatgc taagccaaaa aaatatagag gaggtagaag ataatgaaag
     2401 actcatctag agctgaactt atgagaaaag ttcaggaaac atcttttgca tgtgttgata
     2461 tgaacttata tttagacaat caccctgatg ataaaaatgc tatcaacact tataattcat
     2521 tatgtaatca atttgcgcaa gctagatatg cttacgaaaa taaatacggt cctttaacta
     2581 actttgggta tgctccaagt agatgccctt ggcaatgggc tgaccaacct tggccttggg
     2641 acagagaatt taattattag acatcaagta ttggaggatt gaaaattcat gtggatatat
     2701 cagaaaacta tacaacaccc agttaatata aaaacttgtg accctagaat ggctaaattt
     2761 cttataactc aatttggtgg gccaaatgga gaacttgctg catctttaag atatttaagc
     2821 caaagatata caatgcctac tggtaatatg cgtgcacttt taacagatat tggtacagaa
     2881 gaactagctc acgttgagct tatatgtact atggtttatc agttaacttc tgatgcaagc
     2941 ccagaagagt taaaagctgc aggtcttggt tcaaactatg ctcaaaatgg atatggaatt
     3001 tatccaacag attcaaatgg tgttccattt gatgtaagac ctatagcagt tatgtcaaat
     3061 cccgtaaccg atttacatga ggatatggca gctgaacaaa aagcacttgc aacttattat
     3121 caacttataa acctaacaga tgacgttgat gttatagatg tattaaaatt cttgggtcaa
     3181 agagaaataa ttcactatca aagatttggt gaagctttaa tggatgctta cgagttagaa
     3241 gaatctcaaa aaatgttcta aaatttttat ttgtataact attattattt gataatttat
     3301 aatttactgt tgtgttaaaa tttataaaaa atataaatat aatttatttt ctataaacag
     3361 agaaaataaa taaaaagcag gtatcaatca aatagtaaca tgtattatac tatacattac
     3421 tatcaatatt gtacctgctt tcatattata agcaataaag aaaaatctat ttattaggtt
     3481 ttataataaa aacaattata aaactcttct tgcatttaca tatctacttg aatagtagct
     3541 actatttata ttagatattg ttactttttt tgatgaacct gaagatgcat gtatcatatc
     3601 tccatttcct atgtatattc caacatgact tacagaacca ttatttgaac cttgtgtatc
     3661 aaagaatact aaatctccac ttctaaggtc tcctctattt acatatgttc catacttact
     3721 ttggtctcta gatactcttg gtatactaac tcctacagat tttttcataa cgtactgtgt
     3781 gaatccagaa cagtcaaaac tatttggacc ttctgctccc catacatatg gttttccaag
     3841 tagtgtcttt gcaaaactta ttacactatc agctccttga cttggagaat tagtcgaata
     3901 agatggaatt gaagtatcaa cagaaccttt ttcagcaagg tattgacttg acacatatcc
     3961 agtttgattg ccataactta cttttgtcca tcctccactt tcagaaatac ttccaacctc
     4021 agtaccataa cttaatgtag ctattttaga atgacttgta cctggtcctg ttctaacgtt
     4081 caatgacttt gctgttacaa cctttgtacc ctttacaagg tcatttgaac tgttttcagc
     4141 attttcattt gtagagtcat ttacactagt tcctaaatat ttacttgaaa cataacctac
     4201 tctaccatta gaacttattt ttgaccatcc atttgactca gatatgtatc cgacttcagt
     4261 cccatatcct agtgttgata ttttagaact acttgtactt ggtcctgttc taaaatttaa
     4321 tccttttgct gttacaactt ttgtcgactt aactgaagta tcactacttt cattgctact
     4381 tccaaggcta tttattgcta cataatttcc atgcacataa cctgtgtttc cattatattt
     4441 aacttttacc caagaacctt cttttcctac gtactcaact ttatcacctt tgtaaagttt
     4501 ccccattgat tcatggtttg tacctggtcc ttttctaaaa tttaccgcat tccctgttat
     4561 tgttctgctt tctatactca cactctctac ttcagactca ttagcatatg ctaaattccc
     4621 catagtaggc attattatcg cagttgccat tatactggct gctattctct tttgattaaa
     4681 attcataact ttccactcat ctcctctttt ctactatctt tatttcactt caaaatataa
     4741 aagaaataat tgacactatt aaaatatcaa ttatttcttt tatattaata ttattacaaa
     4801 tttgtaacta tttgtctata gtaatatttg cttaccataa attaccatgt ataaatttta
     4861 atctttaata gcctgtaatt tagtaatata gccatctttt acctttttta aaaatggcta
     4921 tattttttaa cttttttgta actttttaaa atccagttga gccaaaccca ccttttcctc
     4981 tttcggagtc acttaattct tttacttctt ctatgttaat atcatagata ggttttacta
     5041 tcatttgagc aattttcata tgcttttcaa ccttaaaatc atttttacca tgatttatta
     5101 atataatttt aagctctcct ctgtatcctt cgtcaatagt tcctggtgta tttaatacag
     5161 taacagaatg ctttaaggca agcccacttc taggtcttac ctgcgcttct gtcatagtag
     5221 gaagctctat acatatccca gttttgataa gtttagtctc tccaggaggt attacaacct
     5281 cttctataga atacaaatcc ataccagcat cacctttgtg agcaaaatta ggtattatag
     5341 catcatcatt taattttttt acttttaaat tgtacatctt atatctcctt tcaactttct
     5401 ataaatatta taacttaaat ttcgacctct tgaagttttt tatttttttg tggtaatata
     5461 attattagtg ttttaaagga ggatttttta tgaaacttgt acttgctgag aaaccatctg
     5521 tggcaaaaac tatagcatca tttttaggtg caaagaccag acaagatggt tactttgaag
     5581 gaaatgatta tataattact tatgcagttg gacacttagt atcattatat gatatgaagg
     5641 attatgataa agataaatac tctggctctt ggaaaatgaa caatttccca tttgttcctg
     5701 aggataagtt taaatttaag attgatagtt ctaaaactaa acaattcaac actgtaaaaa
     5761 agcttttgaa tagaaaagat gtagaatatg taataaatgc cactgacaat gaccgagaag
     5821 gagaacttat agcattttta atatttttac tggcaaaaaa taaaaaacca gtaaaaagaa
     5881 tcttggtaaa tgaatggaca ccagaagata ttacaagagg tataaaaaac ctaaaagatg
     5941 aagatgaaat gagaaatctt caagctgctg gatatactag gcttattact gactggttga
     6001 ttggaataaa tttcacttct gttgctacat tgaaatatgg aaatgggaaa ctattaaaca
     6061 taggtagggt aattcttcca acagttaaat tagtctatga tagagatatg gaaatactaa
     6121 actttgttcc taaaacctat tatgaaatag aagggcattt taaagctgaa gctggtgaat
     6181 ataaaggtaa atatgttaag ggtaaagaaa gtaaatttga tacacttgaa gatgcaaata
     6241 aaattatagc ttcaattaca tctgaaactg gtaaaatact agataaaaaa gttactatgt
     6301 caaaggaata tgcaccaaaa ctttttagtt tgacatcact tcaaggatat ataacttcta
     6361 agtattccaa ttttacatct gataaggttt taaatgtctg tcaatctctc tatgagggta
     6421 atggtaaggg tggatacata acttacccta gaactgattc cattttctta gaagaaagtt
     6481 tggcttcaaa agcttctcaa actttagata aattaaaggt tggtcttgaa tatgaaaata
     6541 aaattaaatt ttctaaaact aaaagagttt ttgattcttc taaagtagac tcccatagtg
     6601 ctataattcc aacttatata attccaaaaa gtttatctaa ggatgaacaa ttagtttatg
     6661 atgccataaa agatagattt gtagcaaact ttatgcctcc tgctgaatat gagaatacag
     6721 aaattaagac tgaagtagat aattgtactt tcttaacaaa aggtaaggtg cttaaatcaa
     6781 aaggatacct tgaggtatat aataaggaag aaaaaaacga tttacttcca cttgtaaata
     6841 aagatgatgt tgtagatgtg ttagaaataa aaccattaac taagcaaact actccaccta
     6901 aaccatatac tgaggacaca cttttaaagg ctatgaaaaa ttgtggtaaa aatgtgcctg
     6961 aagaagatac aacagtgcta tctggatatt cgattggaac atcagccaca cgtgctgatg
     7021 tgttaaagaa gataagtcaa gtgggctatg taaagaaaaa aggtaaatct tattctataa
     7081 cagaccttgg gaaaaattta gttgaaattt ttcctgttaa agacttattt gatgtagatt
     7141 atactggaaa acttgaaaaa agtttaagcg atattcaaaa aggaaagtat acaagaaaag
     7201 aatatcttac taatatcatt agttttatat atcaaaatgt caatattata aaacaagatg
     7261 cttcaaaaaa tataaacaca gaagcatata ctttcgattc aaaaactaaa aaatttacta
     7321 aaacaaaaga aaaaaaacag aaaaaagatg taactaatac tgaaaaagaa gttgctatat
     7381 caaaggattc taataacaaa aaagaagtta aaaatggtga tgaaaatcaa tctcttggca
     7441 aatgccccat ttgtcaaggt gatgtactag aatttgacaa aggctttgct tgtaaaaatc
     7501 ataaagaatg taaatttgtt atttggaaaa atgataagta tttaagtctt tataaaaaga
     7561 aagttaacaa aacaatggtc aaaaatattt taaaaaaagg tgaaactaaa gtaaaatctt
     7621 taactgctaa aaacggtaat aaatttgatg ctacattaaa atatgataaa aatccagata
     7681 ctggctattt caattggaaa atagagtttg acaactaaat attcataaga aaagaaatag
     7741 cagtagtata ataatctata ttatattact gctatttctt ttcttatgaa gttttaactt
     7801 ttttaatagt gacttttaca atcacactct aacctaattt ctttactatt tctgataact
     7861 ttaatgtatg ttcatctaat aaagttagtt cctcttcaga aagattagat aattttttta
     7921 ctagagattc tttgagaata gcttttactg catcaaataa tttaaatgct ttatctgtta
     7981 attcaaccct aagaatcctt ctatcttttg ggtcaggaaa tttattgact aaaccatgtt
     8041 ccactaatct gtcaataatc ggagtcatat ttgattttaa aattcctaaa ttagaagcta
     8101 tttgagaaat tgatgatgaa tttgatgtca taagataaag aattacctta gcatgtgatg
     8161 gagccattgg acattcttca aaataagctt tcattatatc agagttattt tgaaactttt
     8221 ttaacaaatc gctttcttta aatatattat tttcaagcac aaataaaaaa tctaaaaaag
     8281 agtccgctat tttaagtata ttgttgttat ccatatttgt aacatccctt caatagataa
     8341 tataataaaa cattgttgtt taatatagta taaattataa actattaata aatttaaaac
     8401 aagaaaaagt tattgactca taataattat aaatttataa tcaaattaga gaattataaa
     8461 ttgccaataa agggaagttt tttaattagt ctaaaactaa acttaaatta taagacactt
     8521 taaattctac aacaagctag tgtgcaatat ataataggag gagatatgaa aagatattta
     8581 atagttttct taatgtgtag ttcactgttt ttagttggat gtggaaaaac agaagaatct
     8641 gaacctgaga aaccaatagc agtttctgtt caaaaagcaa taggtggaga aattgaaaac
     8701 acaaattctt tctcaggaac tacaaaggtt aaagatgaaa cagctgttac tgcacagact
     8761 gttggcacag ttcaagaagt gtatgtaaaa cttgggcaaa atgtaagaaa aggagatgaa
     8821 ttattgagta taagcagtcc agagctagaa aatagtgtaa agcaaagtaa agcctcacta
     8881 gatttagcaa aagcaagtta ttcaagtgcc actggtggtt cattagaggc acaagtaaat
     8941 caggcaaaaa cagctcttga caatgcaaag attcaatatg aggagtctca aagaaattat
     9001 gacaataata agattcttta tgaacaggaa gtcattagtt tagaccaatt taaaaaaata
     9061 gaattttctt tagagcaaac aaaacaacag ttagattctg ctcaaagagc atacgataca
     9121 gcaacttcaa aaagtatacc tcaagctaaa gcacttgcta aaaaacagct tgaccaagct
     9181 caagtatcat ataatttagc aatgagtaat ttagacaaat taacattaac atctccagta
     9241 gatggtacaa taacagctaa aaactttgat tcaaaggaaa tgataacaca atcacaacca
     9301 gcttttatta tttctaatcc tagtatacta gaagttgatt taaatgtagc agaatcagat
     9361 ataggtaaat ttaagaaaga tggaaatgta gatgttataa ttgaagacca aaggatttta
     9421 ggaaaaatag attatgtacc ttcagtagtt gaccctcaga catctttata tcctgttaaa
     9481 gtacttgtaa ataatacaaa caacaaattt aaggcaggta tgtctgctca agtaaactta
     9541 agtgttgaaa aagaaaatgg tgctgttaca gtacctaaaa aagcaatatt tgaagaaaat
     9601 ggtaaaaaat atgtatacat agcaacaaaa gacaatatag caaagaaaca cttggttcaa
     9661 actggaattg taacagagga taaaatagaa attaaaagtg gtgtaagtga taaagataca
     9721 gtgattattg gagggataag tcttatttct gacggaacta agatatttcc tgtagaaaag
     9781 gagaagtaga ctatgaattt aacacagact tccgttaaac gtcctctcac aatcataatg
     9841 gtctttttag tattaatagt ttttggtggt attggatata aaaaaatgtc aataaatcta
     9901 atgcctgata tagaaatacc agttgtaatg gtcatgacta cttggacagg tgctggtcct
     9961 caagacgttg atgaacaagt tagtcaaaaa gttgatgaaa gtttatctgc cgtttcaaat
    10021 gtaaaatcta ctatatcaag ttcacaagaa agtgtttcta tggtagttgc tcagtttgaa
    10081 tttggaacta atttggatga aataatgaat gatgttaggt ctaaagttga tgcattacag
    10141 accagcctcc cagatgatgc agctaaacca acagttttaa agctggatat gaatgcacag
    10201 gccataggtc aacttgtcat atctggagga aatgaaaata gttcacaagc cttaagaaaa
    10261 tacgctgaag atgtaattca gcctaaaata gaatccatag atggtgttac ttcagcagac
    10321 cttaaaggtg gaaaaaaagc acaggttaat gtaatagctg acccagctgt tctttcaaat
    10381 tatggggtta gtttatccac tataaaaggt gtcttatcct ctagtaataa aacattccct
    10441 tatggttcaa ttactcaagg tgaggataaa atagttttaa gagcgattga taaattagaa
    10501 tccctagatg atataaaaca gattcaaatt cctgtaaaag gaggaaatac agttagatta
    10561 gataatcttt gtagtgtaga ttatggatat gcagacaaag atagtattta tagatacaat
    10621 ggaaaagaca gtttggtaat agatatttct aaacaacaag atgccaacac tgtaaaagtt
    10681 atgcagagtg catacaaata cgtagatgaa ttgaataagg aaaatcctca atttaaaatt
    10741 aaaattgcta atgatacaag ttcatacata agtgaatcaa taaacagtgt aatgagcaac
    10801 ttgcttataa gtgctgttat cgcatttatt gtaatatttg ctttcctaaa aagtgtaaga
    10861 gcttcactag ttgttgcagt tgcaatacca atttctataa taggtgctat agcaattctc
    10921 tactttactg gtgaaagttt aaatcttatt actgcaagtt cattggtaat ttcagttggt
    10981 atggttgttg ataatgctac agttgtaatt gaaaatatat ttaaatatag gaaaagtggc
    11041 agattgagtc ttgatgattg tgctattgag ggaacaagaa ctgtaacaaa tgcaatactt
    11101 gcatcaacat tgaccacaat agcaatattc ttacctatca tgtttacaga aggtatagct
    11161 aaaataactt ttggggcatt aggtaaaaca ctaatagctt cactagcatt ctcctttata
    11221 ggtgcaatta cactcgttcc tagtgtattt gcaaaattaa gcagaggaaa aaatagtcaa
    11281 aagatgcaag aaaaagatag tcctatattt gataaggtaa gtgaagcata taaaaaatta
    11341 ttaagtgtat gtttgagaca tcaacgctta gttgtaatat caagtttact tatttttgta
    11401 gcttccttat ttggaatggg atttataggt atggacttta tgcctgcagc agacaagggt
    11461 gaattatcta tttctataga acttccaaaa ggtcttagcc tagaatccaa tgattactat
    11521 gtatctatga ctgagaaaaa agtctcagat attccagaaa ttaaaacttt aataacaact
    11581 ttgacatcag attcaaataa tagtaaagca agtatagcaa ttgaattaga atctcaaaaa
    11641 aatagaaaaa gaactactga tgaaatagaa aaagagataa ttgatagagt ggctactgta
    11701 ccagattgta aaataaatgt aagtcaaaac agtagtgtta tgggtggagc tggaggagca
    11761 gacttcacat tagaaatgaa aggtcctgat ttagatgtat taaaaatact gggaaaagaa
    11821 gctgaaaaag aatttagctc tataaaagga tttagaaatg ttgaaacttc tcttacagat
    11881 acaacacaag aagctcaatt tactatagat aaacataagg ctcaagaata tggtgtaaat
    11941 accgctgaaa ttgcaggcat gttacgtaca gcaataaatg gtgactccgt aaccactgct
    12001 actattgatg attacaaagt ggatgtaaac ctaaaattta aagcagatac tattgatagc
    12061 ttagaagata taaagcaact aaaagtaaac tctgcaactg gaaaagaagt tcctcttggt
    12121 gctttttcag atataaaaat ggcagatggt ctacaatcta tatctaggac tgatggagat
    12181 ttttctgtaa atataactgc tacacttgac aatttagatg ctggtactgc tacaaaacta
    12241 gcactagaaa aaacgaataa aatggtaatt cctaaagatt actctatagg tgttggtggt
    12301 gaaacagaaa tgatgaatga atctatggca agtcttggat tttcaatggt catagctata
    12361 gcattagttt atatggtaat ggtagctcag tttgaatcat ttagtaaacc atttataatc
    12421 atgttctgtg ttccatttgc attcgttgga gttgtgcttt cgcttctagt tagtggtcaa
    12481 agtttaagta ctgtcggtat gctaggtata atcttactta tcggtattgt tgttaataat
    12541 ggtatagttc ttattgacta catagagcaa cttagaaaaa caaatcgtaa caatgattta
    12601 attggtttgg tttcaattgg ttgtgcagca agattaagac cagtcttaat gactacagca
    12661 acaacaattt taggtatgtt gccaacagca ctatctcttg gtgaaggtgg agaaactatg
    12721 caaccactag ccatagttat aatgggtggt ctaactgtat ctacacttat aactttggtg
    12781 cttataccta ctatatactt aatatttgat aaatgggaaa ataagttttc agacaaaatg
    12841 cctaaaatat ttggtaagat aacagataaa ataggaagta taaaacgtaa aataataaag
    12901 aaaccaaaat tgaaagctaa agtttaaata gaatggaggg tgattgtatt gaataaaaaa
    12961 atgagcaaaa tagttgcaat tggctgtggt gttggtataa taactgccaa tttaatccca
    13021 gtttacgcag ataataatac ttcttctata aacaaagttg ataattctat tttaagtgac
    13081 tcaaataaaa gaacatctga aaaggaaatt ttgactgcta gagaagctgt tagtgctgca
    13141 cttaaaaata gtgaaaaatt aaaaatgaaa tcagaagaaa taaaaatgtt aaaagaaaag
    13201 ttagaagtac aagatgagtt tgatagtttt actggaagtg acaactcgtt tccatacgac
    13261 caaattggac ttcttaaaaa tcaaagtgaa caggctaaag gctttatgga ggaccaaata
    13321 gcaaatgata ttactaataa atttaacgat ttagtttcaa gagaaaatga gctagacaag
    13381 ataaaaaaca atcttgaaat aaaaactaaa gaaattaaag atatgaaatt aaaaaaagat
    13441 ttagggttag taacatcttt agaaactgaa tcagctgaac ttgaacttca aacactacaa
    13501 aatactcaaa aagctaaact tcaagaacta aaaaacaatc aagactactt taagttgttg
    13561 actaatatag atttaaataa ttatcaattg gataaagaat atagatttga atcttttaga
    13621 gtaggtggct cagttgattc atatatggaa ggtaaggtta atgaatattt aaaatacgac
    13681 caattgattt tagaacgtac agaagaaagc tttaatgata aagatgagaa caaagcagat
    13741 ttaccagata gacctaatct tacaagacca gttgctccta caaaaccaga gcaaggtaac
    13801 ctaactgatg atgaatataa ggttcttacg gataagtatg aaaaagacta caagagatat
    13861 gaaaaagaag ttgaagcata caatcttgaa agacagactt atgctgctgg ccttactact
    13921 tatgcaaatt atcttcaaca aaaatttaat accgaaaatg gtctagtaac attagaagac
    13981 agtaaaaaag ctcttaaaaa agggttaatt gatagctatg ctcaattact tgctttagaa
    14041 gatacaattc agattactaa aaaacaactt gatttatcag aaaaacaatt aaaaaatact
    14101 aaattaagat atgatttagg attaattact ctaactgatt ataaaaaaca agtggtaagt
    14161 aatgaagatg ctaaaaatag ctatgacact cttattgtga attataactc actaaaaaat
    14221 ggtatagaaa aaccttggat acttaatact ggaaaataat tctggtaaat agaaagatta
    14281 ctttaacata cgtttgatta aaaaatagtt atgcacactc gtggctttag tcatggaaga
    14341 ttcagaaggc aaataataga aatagattag taaaaatata tttgcttata aaactgtata
    14401 attttataga ggctatctca aaatagagat aagcctcttt tttattgttt aaattataaa
    14461 aatatattta attccataca aaaagagtgc cttatgaact aaaaagttca ttttgagaca
    14521 ccctcaaaag cttaactcta aatttaatta ttttttatca aatgtctcat ctattttaca
    14581 tctcaccatg ttaacaaatt cagaaatatt atatcctcta agtttgcatc cacatttagg
    14641 acaataatcc aatttcctag ataccaaagc cctacaacta ggttctggac atcttatttt
    14701 tagactctca tctgctactt taaatccaca tcgtgggcat atactacctg gacgtgatga
    14761 tacttccttt ccacatttag gacactttgt caactttatc ccctccagta aactcaattt
    14821 aattgatagt tgaaatttct ctctacaata atatatgcaa tataattaaa tacgtttcac
    14881 ttacaactat ataagttaaa tttttataat aaaaatcccc tatattcaat ttacaaatac
    14941 ttaatttgat acattgaaca ataggggatt ttatttttat ttagttacat attttgcagt
    15001 ctattattat ctaggatttt ttatagatgc ttgagctgct gcaagtctag caattggcac
    15061 tctaaatggt gaacaagata catagttaag tcctacacta taacagaact ctactgaaga
    15121 tggttctcct ccatgctctc cacatattcc tagttttaat tctggtttaa cttctctacc
    15181 taatttagct cccatcttaa ctaacttacc aacaccattt tggtctagta cttggaatgg
    15241 gtctttttct aatatttctt catctacata ttgacctaag aatttaccag catcatctct
    15301 tgaatatcca aatgccattt gagttaagtc attagtacca aaactgaaga agtctgcttc
    15361 ttgtgctatt tcatcagcag ttaaacaagc tctaggtatt tctatcatag tacctacagt
    15421 ataaggaact tctatacctt cttcttttat tatagcgtca acagtttcta ctatcatttt
    15481 tcttattatt tttaactcat ttacttctcc aactaatgga accattatct ctggagaaac
    15541 tttaacacct gctttgattg cttctatagc accttgtatt atagctttag attgcattac
    15601 acatatttca ggataagtta ttgcaagtct acaacctctg tgtccaagca ttgggttaaa
    15661 ctcatctaaa tcaactattc tcttcttaat atcacttact tttattccca tagattttga
    15721 aagttcttct atagtctcat catcatgagg caaaaattca tgtagtggtg ggtctagtaa
    15781 tcttatatta actggttttc catccataac tttaaataac tctacaaaat cttctctttg
    15841 cattggtaat attttttcaa gagctttttc tctttgttct actgtattag ataatatcat
    15901 ttctctaact gctggcaatc tagcttcatc aaagaacata tgctcagttc tacatagacc
    15961 tataccttct gcaccaaact ctatagctgc acgagcatct ctaggattat ctgcattagt
    16021 tctaaccatc atacatttgt gtttgtctac ccagttcatt aatttttcaa aatttccagt
    16081 taaagatact tctgttttct taacatctcc taggtataca tttcctgtag aaccatctat
    16141 agaaatgtat tctccttctt ttataactac atcatcaagt gctcttattt ctttattaaa
    16201 ctcatcaact tttatttctc cacatccagc aacacaacat ttacccatac ctctagcaac
    16261 aactgctgca tgtgatgtca ttccacctct agctgtaagg attccttctg ctttaaccat
    16321 tccttctata tcttctggag atgtctctag tctaactaaa actacttttt ctcctttttc
    16381 atttgctttt actacatcat cagcattgaa ataaacttta ccacttgctg cacctggtga
    16441 tgctggtaaa ccttttgcta taaccttagc ttcttttaat gccttatctt caaattttgg
    16501 atgtaataat tggtctaatt gattaggctc aattctcatg atagcttctt tttcatcaat
    16561 tattccagct tcaactaaat caaccgctac atttattgct gctgctgctg ttctcttacc
    16621 atttctagtt tgtagtatat ataatctttc attttctata gtaaactcta tatcttgcat
    16681 atctttataa tgaccttcaa gtatatgagt aatttttaca aattcatcga atacagctgg
    16741 cattacatct gcaagagttg atatattttg aggagttctt atacctgcaa caacgtcttc
    16801 accttgtgca ttcattaaaa attcaccaaa taatttattt tctcctgtag ctggatttct
    16861 agtaaatgca acacctgtac cactagtttc tcccatatta ccaaatacca ttgattgaat
    16921 atttactgct gttcctaagt tatgagctat atcattcaat ttacggtata cgattgcacg
    16981 aggattattc catgatctaa atacagcctc tattgcaagc attaattgct cttttgggtc
    17041 ttgtgggaaa tcatttttta tttctttttt ataaattgct ttaaattctt ttactatttc
    17101 ttttaaatca tcagttgtaa gttctgtatc aactgtgtat ccctttgctt cttttactct
    17161 gtctaataca ttttcaaatt tatattttgg aacttccata gcaacatcag aaaacatttg
    17221 tatgaatctt ctgtaactat catatgcaaa tctttcattt tgtgtagcct ctgctaagcc
    17281 aacaacacta ttgtcattaa gacctaggtt aagaattgta tccatcattc ctggcataga
    17341 gaatactgca ccagaacgaa ctgatactaa taatggattc ttattgcatc ctaatgtttt
    17401 atttaagtct ttttctaaag tagctaaatg ctcttctatt tcttttatta tctctgctct
    17461 tatactttca ttgttaacat aataatcatt acatgcttct gttgttatag tgaatccagg
    17521 agggacaggt aagcctatct tagtcatttc tgctaaattg gcacctttac ctcctagtaa
    17581 agatttcatg tctttgcttc cttcaccaaa actataaaca tacttagttt ccattgttaa
    17641 atcccccatt aatcttttta ttttaattta tatcattttt attaatagac attattaaat
    17701 ttctatttct ttgtatatct ttagaccatt ttctttcatt atgtctaaaa ttatacctgc
    17761 tgtttcttct attgccttat ttgaaacgtt tattactggg catccaattc ttttcattat
    17821 ctcttctgaa taatctaact cctgaagtat tctttctaaa ttagcatagt ttgctttact
    17881 ggacaatcct aaagctttta atctttgggt tcttatctca ttcagctttt caggtgaatt
    17941 agtaagacct attatctttt tagtatctat ctcaaacact tctttaggaa ttggtatttc
    18001 tggaactaat ggaacattag ctacttttat atttttatta gctaaataca tactcaaagg
    18061 agttttagat gttcttgata ttcccactaa aattatgtct gcttgaagaa cacctctagg
    18121 gtcttttcca tcatcatatt tcactgcaaa ttctatagct tcaactctct taaagtaaga
    18181 ttcatctaat tttctaatta taccagcttc tcttttaggt tttcttcctg ttctcttgct
    18241 tatttctctt agtatatctg tcattaaatc tatacaacta agaccttctc tttcgcaata
    18301 tctttcaaca atactgcata attcttcatc aactaatgta tatacaatta aagcgttttc
    18361 agcttttgca ttctccaaag tttcaagtag tactccttta ttaatcatgt atggaaatct
    18421 ttttatttca taagtttcat tcacataaaa ctgagacata catgcctttg caacttgttg
    18481 tgcagtctcc cctactgaat ctgatactgc atatataatt aggtttttca ccagcatacc
    18541 tccttaattg tcaactattt ctaagaatag cttagttatg ttggtttttg atattctccc
    18601 aacaactctc atatgatttt tgtcttctcc cttatattca acaatcggaa tcgaatctac
    18661 ttcatgttct attatttttc ttgaagctaa taaaacactt gctcctttag ttaaagtgac
    18721 tacatttgga gttcttgtca ttatcatacc tattggcatt ttatttatat cagaacctcc
    18781 tatagttgct tttagcaagt cttttctgga aacaactcca cataactctt cattctcatc
    18841 tataataact atactcccta catcagataa aaacatagtg actattacat catatatact
    18901 ttcatccttc ttagctagta caggcatgct cattatatcc tctactgttt tttcttttac
    18961 acttttacca attaaattta tttcactaac tcctgaataa aaatatccaa cttttggtct
    19021 tgcgtctaat attcctgtca ttgttaatat agctaaatca gatctaagtg tagcacgagt
    19081 cacattcaag ctggaggcaa tactttcact cgttattggt tcattttcct taactatatc
    19141 tataattttt agttgtcttt cattaagctg aataataatc accttctttc attgtgctat
    19201 actttttatc ttttcgcatc ttatatagta tattatataa ctatatatac cattctgtaa
    19261 acagattttt tattgaatac gatatatttt ttactaagtt ctctcgctta aagatattta
    19321 ttatatatta atgggggata tttctttaat taatagaact atattagtat tacagttaat
    19381 attataatac ataattggtc attttaattc attttatatt aaaattttta ataatattat
    19441 tttaggaggt aaaaatgata aacgatatta aaaataaatt caaaaattac aaaccatata
    19501 taaatggata caagaatatg aagagagcat ctgttttaat tccaatagta gaaataaata
    19561 acacacacta tatattattt gaagttcgct caaaaaattt gaagcatcaa cctagcgaaa
    19621 tttcttttcc aggtggtaaa atagaatcag gtgagactcc atatgaagct gttattagag
    19681 aaacttgtga ggaacttgga acttttagtg acaatataga agtaatatca agtcttgacc
    19741 tccttataac accagtaaag tttatcatac atccatactt aggatatata aaaaatatta
    19801 ataatttaaa tataaacaag gatgaagttg accatacttt tttggttcct ataaaatatc
    19861 ttttagagaa tcctccaaat acttatagta atactgtaaa agtttttcca gatgaaaatc
    19921 taccatatgg ttcaattcca aaccaaaaag attataaatt tgaaagtgga agttatccta
    19981 ttctgtttta taaatataaa gattatgtaa tttggggaat gacagcaaaa atactagaaa
    20041 actttttaaa ttttttatca gaataacact cataataaat tatcaaaatt tttacataag
    20101 aaatttataa aagtttctaa atttaacaat tctaaaatat caataactat attttgtagc
    20161 acttttttat tttattattg tttgaatatt tgatggtttt caatgcaatt tcaacataaa
    20221 tactatatat tagtgtatac tgttgatgaa ttaaagtatg ataatctatt taataaagta
    20281 tgaagtaaat gattttaatg tgcctttatg gtaaaattac taataagtaa taaaattaat
    20341 taaaatatat ggaggtcctt ataatgtttg ctgtaggatt tgttgttata attgcacttg
    20401 cctttttcat tttaagtgta ctattattta atggaaaagc cagtatgcta ctagcaggtt
    20461 ataactctat gagtaagaaa gaaaaagcac gatacgataa aaaaaaatta tgtagaagtt
    20521 ctagcattgt tacttttatt gtctctataa tgctatttat tatggcgttt ttgggatata
    20581 gaatagagag tggtcaaatg gacgaaaaaa aaatggttcc cttttctata atatttataa
    20641 tagtcatact ttccagtgta ttttttaata tcctctactc taacaaaaaa tgtaaaaaat
    20701 aaatagttgt aagttgagca gttaattata aaagattgac tgctttttca tgtatattga
    20761 ctactcttat taaatatttt aacatttaaa aatctatgta aaaataatga atatagtaca
    20821 aacttctaat aatgatttta cctttgtggt tacagtccac tatatataat ttaatatgat
    20881 aacagggaat tattaatgac aactaagtaa acttttataa aatataataa aacaaaaata
    20941 gatgtacaat gaaattatat cttatatcaa atttattttt attaatagca aaaacttaca
    21001 ttagcataac atatgacaaa ttcgcttaaa ataaaaattt ttaatataat ttgttctata
    21061 gtacatctat tttgattagt ttacataatt ttattatttt gtacaatatt aattatttac
    21121 taatatccca tttaatgaaa aagttctagg ttcagtaatt ttgaccataa ctaatttacc
    21181 aattaaatca tcatttccac cttcgaaatt tacgagttta ttttgtctag ttctaccagc
    21241 aaacttattt tcattatttt tacttcttcc ttcaactaga acttcaacta ttctatcttt
    21301 atatccatca tttatttctg cagaaatcct atttacagct tctaacaccc tattaaatct
    21361 atcatgcttt atatcttctg gtatttgatt ttccatttta gcagcaggag ttccctgtct
    21421 tttagaataa ataaatgtaa aagctgaatc atatctaact ttttcaacca catctaaagt
    21481 gtccaataaa tcttcttctg tttctcctgg gaaaccaatc attaagtctg tagaaaaagc
    21541 aatacctgga acttcctttt tagctttttc tattatttct aaataatatt cctttgtata
    21601 atgtctattc atttttttca aaaggctact gcttccgcat tgtattggta gatgtaaaaa
    21661 ctcacacacc ttatcacaat ctctaattgc gtatattact tcatcagata tatcttttgg
    21721 atgagatgtc ataaatcgaa ccctttcaat tccttctata tcattcactt ttcttaaaag
    21781 ttctgagaat gaaactggat tttcaagtgt cttaccataa gaatctacat tttgtccaag
    21841 taaagtgatt tctttcgttc catttgcaac aagctctttt atctcgttaa ttatatcttc
    21901 aggagttcta cttctttctc ttcctcttgt gtatgggact atacaatatg tgcagaaatt
    21961 attgcatcca tacattatat ttacaaatgc ttttaattca aattttctat tacttctaag
    22021 accttctatt acttctccat ccacatccca tacatccact aacattttgt cagaatttat
    22081 tgattctgta agtaattgcg gaaatttata tagattatgt gttccaaata taaggtctac
    22141 atgtttatat ttttttctta attcctctac aacatgtggt tgttgcatca tacatccaca
    22201 tacagctatt ttcatatctg gattttttcc ttttagatgt tttaattgtc ctaagttacc
    22261 atatactttt agctctgcat tttctctaac tgcacaggtg ttatatatta ttaaatcact
    22321 ttcttcaacc atcattgaca tttggtatcc catctcttct aacattgaac ataatttttc
    22381 tgaatcatgt tcattcattt gacacccaaa ggtttgagta aacacgagtt ttctctttcc
    22441 agttctgttg aagtattctt cattttgtct gtgtatttgt tctatatatt tatcttgttc
    22501 ttgtattttc tctataggta ttgttacttg ttttcttttg ctcacgtcta ttcctcctag
    22561 ttaatttctt atattacaca taaccttaat tatatcaaat aagaaaaatc ttttaaagat
    22621 attattattt ttctagttgt gttttaatag taaatctaat gatttatata tactatattt
    22681 ctaaaatata aaatcatata attattataa taataaaaag atgttttaaa ttaaaactta
    22741 aaacaccttt ccttaaataa aatatatgat ttactattaa atacaattac atctattcat
    22801 tttcaattaa ataacaatca tttaatatta aatattgacc ttcctcaacc gttatatatc
    22861 ttcttcctga aaaagtatca ttagtaacta ttccctctgc attacctaag ctatcatttc
    22921 taacttcata atatgcatca gaagaatttg acattataat atattctcca gcaggaattt
    22981 ctaatccaat tttatacata ccatttccaa acttaccatt cttggccttc tgggctttag
    23041 catactttgc taatgtcatt gttgaatcct caattttaat atattgacca ttctttaagg
    23101 tgatatatct agtcccacta aaaatatcat ttgaaagtat actatcagca ttacctgtac
    23161 tatcacttgt aacttcataa taagaagcat aacttcctga gtttgaagtt attaagtatt
    23221 cacctgctga gatatctttt ccaaccttat acattccatc ttttatagtc tttgtagtag
    23281 gtttaacatt ttttacaacc ccagtattaa tatctccaat tccatttgtt atatttaaac
    23341 actgttgtac tatatttgaa tctatatatc ctatagaagt tatcttctta gcatttttta
    23401 aaatgctttt attgctacca tcattaacta aaaccattgg accatgtttt gataaagaag
    23461 atcctacaag tgcatttgtt aattcataag ctccagctat ataaaactct ctagcatcct
    23521 tataaaactt atttattatt gctttattag tttcaaaacg agtactcccc cctagtctag
    23581 tagatttagt attgtttact aaagtggtac tcattgaagc tgttcctcct attacataag
    23641 actttaaacc actagttgaa aaaggtatac tttgtccatt agtaagaatt attggtgctt
    23701 tatctcttgc tgctacagat gcaatactta tagcatctgc ttctccttta tatgcattgg
    23761 ttagcataac agtattgact ttttttattg aatttatttc ttttgcaaca ttataacttg
    23821 tttttattct gtcatttccc ttaattctta caactttcat tttcttagat tttaatgaat
    23881 tttcgacaga tgtactaata gaatatgttc ctccaattat ataaactttt gatacatttt
    23941 ttaatcttgc agatgtctca gttggtatag tatttttttc agttaatagt ataggggcat
    24001 ttaaagctcc tgctagtcca cttgcactta atccatctgc aatagagtta ctagtattaa
    24061 ttaatatagc agttttatat gcatttttat ctgctatttt acctgcgatt tcatacttat
    24121 tttttccttg tattttttct acagatgata ttgcatctac taattgaata tttgcaaata
    24181 caaaagaaat acttaaagtc aatgttaata ttcttttata gaatttcata ataaaattgt
    24241 ccccctcata ataaattttt aatatataaa tacat
//
LOCUS       DAMJYX010000051        24071 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJYX010000051 DAMJYX010000000
VERSION     DAMJYX010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.051"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     regulatory      199..300
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            373..1716
                     /locus_tag="PC479_002146"
     CDS             373..1716
                     /locus_tag="PC479_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2804689.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1895..3259
                     /locus_tag="PC479_002147"
     CDS             1895..3259
                     /locus_tag="PC479_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2804690.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3437..5302)
                     /locus_tag="PC479_002148"
     CDS             complement(3437..5302)
                     /locus_tag="PC479_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2804691.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5538..6755)
                     /locus_tag="PC479_002149"
     CDS             complement(5538..6755)
                     /locus_tag="PC479_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2804692.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6955..7818)
                     /gene="aroE"
                     /locus_tag="PC479_002150"
     CDS             complement(6955..7818)
                     /gene="aroE"
                     /locus_tag="PC479_002150"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2804693.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7900..9822)
                     /locus_tag="PC479_002151"
     CDS             complement(7900..9822)
                     /locus_tag="PC479_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2804694.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9886..10734)
                     /locus_tag="PC479_002152"
     CDS             complement(9886..10734)
                     /locus_tag="PC479_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2804695.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11147..12028
                     /locus_tag="PC479_002153"
     CDS             11147..12028
                     /locus_tag="PC479_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2804696.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12236..13099)
                     /locus_tag="PC479_002154"
     CDS             complement(12236..13099)
                     /locus_tag="PC479_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2804697.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13254..15188)
                     /locus_tag="PC479_002155"
     CDS             complement(13254..15188)
                     /locus_tag="PC479_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2804698.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15300..16313)
                     /gene="galE"
                     /locus_tag="PC479_002156"
     CDS             complement(15300..16313)
                     /gene="galE"
                     /locus_tag="PC479_002156"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2804699.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16337..17206)
                     /gene="galU"
                     /locus_tag="PC479_002157"
     CDS             complement(16337..17206)
                     /gene="galU"
                     /locus_tag="PC479_002157"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2804700.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17404..17724)
                     /locus_tag="PC479_002158"
     CDS             complement(17404..17724)
                     /locus_tag="PC479_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804701.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17714..19705)
                     /locus_tag="PC479_002159"
     CDS             complement(17714..19705)
                     /locus_tag="PC479_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804702.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19828..22371)
                     /locus_tag="PC479_002160"
     CDS             complement(19828..22371)
                     /locus_tag="PC479_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2804703.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22649..22983)
                     /gene="rnpB"
                     /locus_tag="PC479_002161"
     ncRNA           complement(22649..22983)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC479_002161"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23115..23792
                     /gene="srtB"
                     /locus_tag="PC479_002162"
     CDS             23115..23792
                     /gene="srtB"
                     /locus_tag="PC479_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2804704.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23952..24026)
                     /locus_tag="PC479_002163"
     tRNA            complement(23952..24026)
                     /locus_tag="PC479_002163"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23992..23994),aa:Thr,seq:tgt)
BASE COUNT         7694 a         4161 c         2712 g         9504 t
ORIGIN      
        1 acaaactcat ttataataaa cttccattaa tagtataaaa caagttagaa taattgacat
       61 tatactcata catattcaat tattggaaaa agtaattttt ttcaataaat aaccttaatt
      121 ttttataaaa tcgacaaaaa aacgaataat tttattatat ttattgtaaa taatacaata
      181 ttacgatata atacaacaga aaaacaaata gaaaatgttc atataatatt agtaatacgg
      241 tctaatagtt tctacaaact caccttaaat gggttttcta tgaatagaat gtcaactctg
      301 aatatatgtt ctattctatc tatatgaata tatatttttt tgtttatatc ttaaagtttt
      361 ttggaggtaa aaatggtacc aaacactaac aatgtacaac caggtggaaa taattcatca
      421 ttaagctttt tagacaaaat gtttaaattt agcgaacgtg gctctagcct taaaacagaa
      481 gtaataggtg gagttactac attcttaaca atggcataca tagttttcgt aaatccagca
      541 atattatctg aagctggaat ggataaagcc gcccttataa cagttaccat actagctaca
      601 gccattggta cacttatatt tgcgatttta ggaaatgcac catttgctct agctcctggt
      661 atgggattaa atgcattttt cacatactct ttggttatag gtgcaggaat cccatggcaa
      721 acaggtcttg gggtagtttt tctatctgga tgtgtcttct ttacactcgc agtaacaggt
      781 cttcgaaaga caattgcaga cgcaattcct agagaattgg cagtagcatc ttcagcaggt
      841 ataggtcttt tccttgcttt tgttggttta aaaaatatgg gtgttataat agctaatcca
      901 gatactatag tttcacttgc agattttaca ccaacagttt taatagccct ttttgctctt
      961 atcttaatgg caatactaga atataaaaaa gttaaaggtg gaatattgat aagtatagta
     1021 gttgcaacaa taatatctgt tatatttgga tttgtggagt tgccaacagc aataatctca
     1081 acacctccta gtatagcacc agttgctttt aaattagata ttgtaggtgc attgaaattc
     1141 tcattaattg gacctatatt ctctttcatg tttatagaca tgtttgattc attagcattt
     1201 cttattactt gttgtaaaca aatgggtctt gaagatgaga atggtgaaat aaaagattta
     1261 ggaagaatgt tatatgcaga tgtttcttct actttaatag gctcattctt aggaactagt
     1321 actgttacaa catttggaga atctgcttct ggtattgcag caggtgcaag aacaggactt
     1381 gcttcaatag taacagcagc attattctta gctacactta tatttacacc attacttggt
     1441 atagttccta cttacgctgc agctcctgca cttgtaatgg ttggtgtgtt tatgtttgaa
     1501 agcataaaac agattgattt tgctgatagc aaaatagctg tacctgcttt tgttacagtt
     1561 ttattgatgc ctttaactta tagtataagt attggtttat gctttggatt tatttcatat
     1621 ataataatgc atgtagtagc taaagaaact gataaaatca gtattacact ttggattata
     1681 ggtttacttg cagttgtaaa cttagttcta agttaatatt tttaaatagt tctaggtcaa
     1741 tattttaaat tatatagaat aaaatcttat ttattttaaa tttataaata gcaaaatcac
     1801 tttatatgta aattgatttt gctattaact ttgtatagaa aatttattat cataatatat
     1861 agatataaaa atatatagaa aaggagtggt gtttatgtca caattactta taaaaaatgc
     1921 atatctaatt actatgaatg cagatagaga ggtatatgaa aatggaagca tacttattga
     1981 aaataatatt ataaaagagg ttggtaagtt tgatattaat attgtagata aagatgcaga
     2041 gatatatgat gctaaaggaa aaatactaat gccagggcta gtcaatactc atgttcactt
     2101 atctcaacaa ttaggacgtg gtcttgcaga tgatgttgta cttttaactt ggcttagaga
     2161 aagaatttgg ccatacgaaa gtagcttcaa ttatgaagat tccttgattt catctactgc
     2221 atgttgtgtt gaattgataa aatctggtgt cactacattt ttagaatctg gtggtcaata
     2281 tgtagatgct atggttgaag ctgttgataa aactggactt agagcctgtt tagctaaatc
     2341 agttatggat acaggagatg gtcttcctga ggcatggcaa aaaactactg atgaggaaat
     2401 caacactcaa ttagacttat tccataagtt taataataca tctaatgaga gaattaaaat
     2461 atggtttgca ttaagaacta tttttaatac aactgaagaa ttacttgtta gaactaaaga
     2521 actagctgat aaatacaata caggtataca tatgcatatt gctgaaatag ctgatgaaat
     2581 ttcatttgta aagcaaaaca atggagttgg aacagttgaa taccttgata aactaggtgt
     2641 attaggacca aatcttttag ctgctcatac agtttggctt actcataagg aaattgattt
     2701 atttagatta catgatgtaa aagtttctca tgacccaggt tcagctatga aagtggttct
     2761 agggtttgca tcaattcctg aaatgttaga aaaaggaata cctgtttcaa ttggtactga
     2821 tggagctcct tcaaataata gaatggacct tatgagggac atgtatctta catcactaat
     2881 acataaagga agaactttaa acccaactgt cgtaccagct gaagaagtct tagaaatggc
     2941 tactataaat ggagcaaaat gtgctttact agaaaaagaa attggtagtc ttgaagtagg
     3001 aaaaaaagct gatatgatta ttctaaatcc agatacaatt cattgtttac caatgcataa
     3061 tccaattgga aatatagttt attctatgac tagtgaaaat gtagactcta ctatttgtga
     3121 tggtaaatgg ttgatgaagg aaagaaaagt attagttgta gatgaatctg aattattaaa
     3181 taaagttaaa aaacaagctt ctaaaataaa agataaagca gaagttaaaa ttccatctaa
     3241 atttaaaatt ataaaataac ataaatttca attgttttgt aaatatgaat atataaatat
     3301 atttatatac atcatatata caaaacaatt gtttttttat tttatattca ttagcattgc
     3361 tcataaaata caccataatt ccagaaaaaa taaccaatca gttaggatta catcatctaa
     3421 gcaatatata aacgtcttag tttataagtt ttattctact cttatctaaa accatcttta
     3481 aagagttttc aactgtttca attacatctt tataactcaa gtacacattt gcgtagatac
     3541 aatcttcata taaattatat tcagttactc tacaatacag cagttttcct ccatctaata
     3601 aattgctcaa aatatagttt tcttttcttg ggacatagcc tatcacatat ccagtttcac
     3661 caactatttt aatagctttt tcatcatata gattatcttt ctctctagtt aaatagtata
     3721 tcttactttt ctccaattct ttttcaagat attctctatt tctaaacctt gaaccagcaa
     3781 cagctattga agtcaaatag atatccttac catgttcatc tacttttatt ttttcaatat
     3841 ttacaaattc tttttttaaa ttattacttt catataatac tcttcttaat tcatccttca
     3901 atataacatt tttttctttt tcatacgctt ctttaacaat ttttaattct tcaagagata
     3961 gtttctctct gtatcttttt aagatattta tggattcttt tcttacttca tttatatttc
     4021 caaatattcc ttttaaagat atattctttc cttcaggata tagtgattta cttcctttaa
     4081 gcacaataaa aaataacata tcatcataat attcttgagt caatttatct ttttctatat
     4141 ctttcatttt tccaattaaa tcatctattt taaacgtctc ttcaaaaaaa tttaataatt
     4201 ttaatctaga acttgtcgtt ccctttttag atatatgcca atatacctca aaatctctaa
     4261 tatctctatt tagatatggc aacaagtcat caaaagataa gtttacatca taaatttctg
     4321 acatttttat aatatcttct gtttttccac tagcatctct taatgcttca aaatatatat
     4381 ttttccattt ttcactgagc aagataccat ttatttcttt ttcaaattca atcttattgc
     4441 actctatatt ttcatctttt aagacgttta tagctattag gaaaattgaa tataaacttt
     4501 caaagttagt cccataattt actacagtag gtaaaaatcg attcactaat tctaatttat
     4561 tacctatata tttaaattct acacttagca agtaattgca tataagacgt gctaaattat
     4621 ttatcttttc tttatctaaa tctcgttttt caagatattc attcaaatct acaattgaaa
     4681 taatatagtt catcaataat cgttcgtact ttgtatcctt ataaccatct tcaattagat
     4741 aggaatttat tttagaatct aaattttcca tattaacaat agcaaatact tttattgaac
     4801 ctgtagcctt tttagataaa ttaaataaat atgtattgta aaattcaagt tttttaatag
     4861 tattactcag ataaaatacg tattctccag atttagaaaa tgtatctaca acttctctta
     4921 agttttctac atttagatat ttctcactta aaactaaccc tagttttaca tactcaactt
     4981 ttttactctt atatattaat tctttagtta atttatataa ccatttctca tcaataatca
     5041 tttcttttac caaagtattt atctttttca taaaaatttc gaaataacaa attaaagact
     5101 tagaattact accctccaat aaataaattt cattagattc acttttatac atatttaaaa
     5161 tttcatctac tagattattt gcaagctcta cttgttctct taacggtata ctttcatata
     5221 tacataggta attcatttca aatgattctt tgtatccatc ataaccatta aattcctgca
     5281 atcgcttaaa aacattctcc accaaattat cctcctttac atgatgtaat ttatattgat
     5341 tatactactt atttcatcaa gtttacattt atacgcaaaa aaatattact agaaattgaa
     5401 attctagtaa tatccttatt attaattatg taaataccct atcttataac tgtaaataat
     5461 atccttatta attttaatta cttttaataa aaatctatcc tagatttaga cttctctatg
     5521 catatattat actaatatta gacatcataa tcatacactt tttctttttc atatctcatg
     5581 tttacaaaca atgcaagtaa aattccaaca aatgttattg ccacattaaa caatacaaca
     5641 tattttggtc cattaactcc ccctgatttt gtaataatac ctgctatatt taaaataaca
     5701 tagtttgcta tactagatgc aatcataact atagatgtta tttttccttt attagttgga
     5761 aacatttcat ttgcagttga cgttgttaac tgaagaacac ctcctgctgc tgaataacct
     5821 attacaaatc cccctaacat acaaatagtt ggagtttgaa caaaatatac aactaatagc
     5881 ataagtaaag ctattatagg atatataact aatattctta ttggtttaat atatttttta
     5941 ataagtaaag aagttattaa tactgcacat attactccca ttgagtaaaa ggattgaatt
     6001 tttgtagggt cagccatccc atacaattta cccaattcct gattacaatt taaccataat
     6061 acaaatgtag atgtacatgt aaatccaata caaacaagag ctatacttgt tggtgtaaat
     6121 ttcatcttct ctgatttaac tgttttgcct ttgttatcaa taacattatt agctggtgga
     6181 aatggtaaca cagctatcaa aaaggcatct actacaataa gaatcgctgt tactataaaa
     6241 ataactttaa atgatattga atttgctgca acaaatccaa ttataaaagg aagcaaaaat
     6301 tgtgctaaag ctattgtaaa tttagtaaac atatttgcta ttgttcctaa actagcaaat
     6361 atctccatac aagaaggagt tacacaggta tctaagaatg aatttgctgc ccctccaatt
     6421 attgcaaata catatgctat ataaaaattt ggtgaaaata caattccaac aaagaaaatt
     6481 gcatgtagaa agtttcctat aagaccactt acttttcttc catacttatc tgaaataggt
     6541 cctgcaaatg gataagatat aagtctccct aatcctagtg cagcaataac agctaaaaca
     6601 atacttacat ccaatgttcc atctgataat gttttagccc cccacattga tgaaaattct
     6661 ggtttgtatt gtcctaaaat tgaaattcca ataccaagta aaaaatatga aaaatacaac
     6721 gctatggcag taggataata ttttttattg ttcataattt ttctcccatt cttgttaatt
     6781 tattaacatt tttgtgtaca ataaactcat ccccataatg gatttaaagt atagtcagtt
     6841 aaagcttcaa atttaactgg actatgaggc aaaacccact atgacaattt gtaatttatt
     6901 attaaaaata ttgaaatacg tatgtttttt cattgtttaa gatattactc ttatctattt
     6961 aaagcataat tcattaactt cttctacagg catttctaat ccagtatata gattaaaagc
     7021 ttctgctcct tggtataata acattcctaa tccaccaata gccttacatc cattagcttc
     7081 agcatcttca atcatttttg ttttcttagg attataaacg acatctgtta ctactaaatc
     7141 ttttcttaaa acactagtat cttttatatt tgtttcattg tcataaggct tcataccaat
     7201 aagagttgca tttgtcaata tatcacttga ttgtatctcc tcataaagtt tgtttgtatc
     7261 ttctaaatca tatagattaa caacacattc tggtacatct ttttttatat tctcaactgt
     7321 ttgctctgct cttttataaa aatcatcctt aggattaaag atagatattt ctcttgctcc
     7381 atctaaagca cattgtactt gtatagcagt tgctgcacca ccagcaccca taattgtaat
     7441 ctttttacct ttaacttcta ccccattttc ttttaaatta cgaacatagc ctactccatc
     7501 tgttatatga ccaactaact ttccattgtc atttacaatt gtattacatg ctccaattat
     7561 acgagctgct ggagaaagtt catccatata ttctgtaaca gcagatttac atggcatagt
     7621 aacattggct ccttttatat taaaagtttt tattgctaaa agtgcctctt ttaccttatc
     7681 tactgtgata tcaaaggcta aatatctata atctaaatct aatttcttaa aactataatt
     7741 atacatcact ggtgatttag agtgaccaac cggtgtacct attaatgcaa ataatcctgt
     7801 tctacctgaa atttccataa ctataatctc ctctcaatat tatcattttc tttgcaaata
     7861 acctattcag ttatttacaa agaaaaattt atttattatt tatatattta atgctacttc
     7921 aaaagcttct tttgttgcat ctaatgccct acgagctttt actgcatcac caacaatata
     7981 agtttctttt acaacttctt ttataacttc acttaatggg tcgtaattac gagaacccat
     8041 agcaagtact actgaatcaa aaccttttaa atcatgctct gtgccatctg taagtaaata
     8101 gtcgacacca tcatcataaa atttagaaac ttttgcacca gtaatggtat gtatattata
     8161 attttcaaaa tcattcataa ggaatttacg gtgttctgaa attacatctg caccaacttc
     8221 ttctctaagc tcaataacag taacctcatg ttcttgttct ccaaggaagg ctgccacttc
     8281 acatccaacc attccacctc caacaacaag tacttttttc ccacaagatt ttttaccatc
     8341 aagaaggtct acagcatgaa ttaatcctga atcattgatt cctggtatag gaagtacaag
     8401 tggagttgct ccagtagcta taataactac atcaggtgat ttctccttta taagttctac
     8461 tgtagcttct gtattagtac atatttttac accatattga ttacattttg aaatataact
     8521 tctaactaag ttagtaatat ctcctttccc tggaggatat gcagctaaac gcatttgccc
     8581 accaagaact tcagattttt caactaaagt tacattatgt cctcttttag cacataccca
     8641 tgctgcaagc atacctccaa cacctccacc aacaacaaga acttctttgc taatttcagc
     8701 tggcttaagc tctgcttcat gtcctaatat aggatttgca agacaggcta taggtttgcc
     8761 ttggaacata tttggtacac atccttgtaa gcatgcaata catggtatca tttcgtctag
     8821 ttttccattt ttagctttat taggcatttc tgggtcagcc aagctttgtc taccgaatgc
     8881 tactaaatca catcttcctt gtctgaccat taactcagca aactgtggct ctgtataacg
     8941 tccaactgtt attactggta tactaacagc tttttttatt tcagtaacta attctgcact
     9001 gaatccagca tgtaaagtcg taggagccca catatattca tcatgtatat gtatactacg
     9061 tgaaacatga agtccatcta ttccacaatc ttctagatat gctgctatta ctgaactatc
     9121 gtgtacatca attcctcctg gaacatcatc agtgctgttt atacgacata atattgctag
     9181 agaatttcct acttttttac gtatattttc aatgataaga cgaggtaatc tcattctatt
     9241 ttcaaaacat ccaccaaact catctacacg cttatttgtt ctttgtgata taaaactact
     9301 aaccagatat ccatgagcgc agtgaacttc aacagcatct gcaccagctt tttgtgcacg
     9361 taatgcagca tctccataag attctataag ttcatatatt tcttctgttg ttattgctag
     9421 tggtgtatta cgaccatttg atgcaggtat tgcagaagca gcttttaatg gatgtcctgc
     9481 tactttttca tttccttcag aaccagcatg ttgtaattgt atagatacct tcgaacctgc
     9541 atcatgacaa gcatctatta catttttaaa actatctatt gtgctatcat catataaaca
     9601 aggcttacga ggacctcctt ttgctttttt gtctacaaca ctagattcta tagttattaa
     9661 tccaaaacca cctaaagcac gctcaagata ataagcctta gaagtttcac ttaaagtacc
     9721 atctgtgttt gcaaaattgt ttcccatagg gggaactaca aaacgatttg gaactgtcat
     9781 tgaaccaatt cttataggtg taaacataga actaatcttc ataaggtttt ccttcctttc
     9841 aagttttata ttataatact ctgtatattt ttatattata taagtttatt ttggagatac
     9901 ttcagaccat gctttatcca aaactttttt agttgcatta aatacagttt cagcagcagt
     9961 ggataaccct ttagatatta gagaatcaga tattacttct gcccctatga ctcttggtga
    10021 tatattgtgt tcttttatta ttttcacaaa ttcctctgtg tttccatatc cttcacctgg
    10081 agccaaacgg tcatgtagtg attcatccct aagtttttca taaggagttt ctaaaacatc
    10141 acacacttga attgatacta tcttatctgc tggaacaggt gcaaggtcct ctgctgtctg
    10201 ctttgctcta gcccagtgcc aagtatctaa tataagcatt gcattttcac gattacatcc
    10261 tttcacaaca cgccatgcag tagccaaatc tggtacacca ctatatggca taaactcaag
    10321 acctataata agttcacctg ctctgtcaca taattctccc aatgcagtta taatttgttc
    10381 ttctggtatc atctctaata atccacaatt tatatgttta acattaaata agtgagccat
    10441 atgataaaca ttttgctctt tttctctttg agcttccgtt ctatcttctt ctcttcccca
    10501 ttgagttata tattctactt ctgtaacttt catatcatat tcattaagaa ttgcaatcat
    10561 ttcttcatct gtaacacctg cattttttgc atcaatataa ttttcagcac gcaatccaat
    10621 accatcaaac ccagcttctt ttgcaatacg aactctatca cggaatgaaa cttctgttcc
    10681 caatgtataa gaactaattg taattggaat attaagattt ttttgattat tcatagctta
    10741 aatttgcccc ctttattttc tttataatta atagtatttt tgattttata tttttattta
    10801 tcctactagt agtattattt taccttaaaa ataaaagtat tttttgattt ctatttatct
    10861 agattttata aatttattgt atcacgagac tttactaata acaaattgat aatttcctat
    10921 taattcatag attttgttta tagattttat ttataagata attcttgtat ttttctatta
    10981 ggtcgtataa atatatttat cttataattc agacaaaaaa cgataaattt ttttaaaagc
    11041 ttttattctt actctataat catatataat aaaaatgtaa aaatatctta aaggttaact
    11101 tctacaaata tataaataaa aatacctatg aaaggatgaa caaaaattga atttatatca
    11161 cttacgctat tttgttacat tggcacattt agaacactat acaaaagcgg cagaaaattt
    11221 atctataaca caaccaagct taagtcatgc tatttcttta ctagaaaatg aacttggggt
    11281 ggctctattt gaaaaagaag gtagaaatat agtccttact aagtatggaa aaatattttt
    11341 aaaagatgtg gaaaaatcat tagaaatttt agattcaagt gtaaaatcat taaaaataac
    11401 tggtactggt gaaggacaaa tagacttagc ttttcttaga acacttggta ctgattttat
    11461 cccagacata gtgcacaaat ttttaaaatc taatcctgct aaatcaatag attttaaatt
    11521 tcatactggt gtaactactg acattattca aggtcttaaa gaaagaaaat atgatattgc
    11581 attttgttcg aaacttgaaa aagaaaaggg aattgaattt atacctgttg ccaaacaaga
    11641 tttagtacta attgtaccat atagtcatcc actagctgca aaggatacta tagatttaaa
    11701 agaaaccata ccttatcctc aaatagtatt taatcagaga agtggtctta gatatataat
    11761 tgatgatatg tttaaaaaaa taaatcaaca gcctaatata gtctatgaag tagaggaaga
    11821 ccaagtaatt gctggattag ttgcaaaaaa tttcggaatt gctgtagtcc caaatatgaa
    11881 tatgcttagc tttacaaaag taaaagtaat tcagattatt cacccaagtt gggaaagaaa
    11941 cttttactta gcatttataa aagatagata tttgcctcct gcaattaaaa actttaaaaa
    12001 ctttgtcatt aaaaacgctc aattataatt tattaataaa ggggttattt ttaaatatcc
    12061 tcttttattc tttttataaa ataaaaaaat ctatactttt aaaagtatag attcctaact
    12121 gacagtcggc atccgagtct cagtaattat cgttatacaa cagcaactgg attcagttcc
    12181 tgaataacta ggttacttac attatatcag attttttaaa atttgtcaat tgtatctaac
    12241 gcattttttc aagccatggt ccaacaggct ctacaatagg aacttcttta aatccccaat
    12301 ttcttatatc attcacactc gttggagtat atattttgtt tctgacagat ttgaatattt
    12361 tgtttttttc tccatattgc atacactttg gattagcttc tattaaccct cttcttgctc
    12421 tgctaagagg attacataaa atatctgcta tttctaaacc ttctctatag tcagcatctt
    12481 cttttttggc aattataaaa cccttaacct tatctcttaa ttcttcatct atatctaagt
    12541 atattgttcc attattaaat acatcaaaaa acgccttttg tacttttaag tttgagacat
    12601 catcccttga ctctataact attcgtgcag attctacagt ttcattattt acaaatgaat
    12661 aaaaattctg aagaacatgt ataaaagcca caacatacgg ttcctttgaa ggtgcataat
    12721 atttctttaa tctagcttta tctactgtta ctgatattat agtacactct aatctagata
    12781 gaaataaagg caactcatca tagaataact taatctgtga atcatcaaac ttctcaaatg
    12841 ctcttgattt tcttgatata tctgtcaaat gtattcctat gttactggtt ccaaaacaat
    12901 actctttaaa tgcatccatt tctttcctca aagaacatac ataatcgcct tgacttactg
    12961 catatttata ttcaaaaatt actcctgtca atgtaaatgg gtcttcatga tttatatcag
    13021 ctcttccact ttcatcaata aacattatat attttttctt tataatctta ctcaccctct
    13081 taacatatga tgatagcatc tatattcatt ttatatgagt tttaaaaata ttccaagatt
    13141 tttatataac ttaaacttaa ttttatacta aaaaattgag atgtaaaaca atcaagcttt
    13201 tattgattga atttcacatc tcaattataa ttatttttca atatatattt ttactatttt
    13261 gcaagaatat ccattaattt atccattact atataagata ctccgcctcc tatttgataa
    13321 ggagtttcaa cttttttatt tgcaaatact ctttgttgct ctgatgataa tcctgaatta
    13381 tccattaaaa ctattggtga atgtaatttt acagctaatg gtccagctga taaagcatct
    13441 actaaagcat ctgatttact aactaataca gcctgtaaat cagtatctcc atagaatttt
    13501 tctatcactt tggcattagt atctactcta ctatctccat atattctatt tcctgaaata
    13561 tcttcagtag ttatatcatt catttttgct attactgaat cgttaattgc acttggtcca
    13621 cctataaagt atgcattttc taattttctt tctgttagcc atttataaga ttcttctgta
    13681 attccatctt tattgactaa tattataggt tgtttttctt ccccagcttt agaagctaca
    13741 gataaagcat ctgcctcccc atttccagaa accatatatg cagtttttat ttcttctcga
    13801 tttccaagtt cttcagctat tcttgtagca gtttgatatc tatcttgacc tgctattctc
    13861 tttgtttctg tgttgaaagt agtttttatt tcttgctcta atttagaact tattgcattt
    13921 tctccaccta tcaagattac tgttttaggt gctaataact tcatttcttc aacagtttca
    13981 gttggtaaat tagctgtatt agtaagtagt attgttgagt catagctact tgctaaagga
    14041 gttgctgtta taccatcagc tattgcatta ccatttacta atattacagt atctgaacta
    14101 tcagcattcc agtttccaac tgaacttatc tttttagctg tatcaaatct atttgcacct
    14161 ataattgtat cccatgtagg atttatagtt ccatcttcat taaaagtata atctttccca
    14221 tctatttttt gagaaccagt tagcatcttt ccatctttgt ctaaatagta agacttacca
    14281 tctatttgtt gccatcctgt ctgcattatt cctgttttgt caaagtaata cttattacca
    14341 tctatttctt gccatcctgt tactgctaaa ccatcagatc ctagatagta tttctttcca
    14401 ttcttttctt gccatcctgt ttgcattact ccatctgagt taaagtaata agtttttccg
    14461 tctattactt tccatccaga tactcttgct ccatcagttc ctaaataata ttttttgtta
    14521 ttaattgttt tccatcctgt ttgcattact cctgaagcat caaagtaata catttttcca
    14581 tttactttta gccaactatt cttttggtat gttccatcag attttacata atacttctta
    14641 ccattttctg ttttccatcc tgtttgattt agatttatct tatattttgc cgctatcttt
    14701 acatagctgt cgttactata atctataatt acatctgaac ctacaggaac ttggtcaaat
    14761 aaccatctaa catctttatt gtgcattctt atacatccac cagaaatatg ctttcctata
    14821 gaactctcat tattatttcc atgtattcca tatgtatctc cataagttcc ttttaatgct
    14881 aatcccatcc atctatctcc taatggattt cttggacttc ctccaggtat attaccttta
    14941 taatatggtc tattttttat cttgttaact acttttgttt tccctgttgg tgtttctgac
    15001 ccctttttac ctgttgcaac tctaaattct tttacaagtt tattatttac aaaatatcct
    15061 agagtatttt ttcttgaatt tactataatc acatgtttac tggctgcgct tgattctggt
    15121 acgccacatg caaatactaa aactaaagtc agtgctagta ctaaatactt ttttagactt
    15181 tttttcacta taacttcccc ctcttttaga aacattacta catgtgtcta attataacat
    15241 aaaattacca ataccttata tttacaaatt tgttacattt atacatataa gtatgtattc
    15301 tactcttcat acccattagg attcatactt tgccatctcc atgaatcttc acacatctct
    15361 tcaagttcat acttagcttt ccatccaagc tctttttctg cctttgaaga atctgcataa
    15421 cacatggcta catctccagc ccttcttcct acaatcttat atggtatttc ttttccactt
    15481 gctttagaaa atgcttttac taaatcaaga acactatacc cctttccagt tcctaaatta
    15541 taaacaacaa gtcctggatt ttcttctaat ttttgtaatg cttttacatg accagcagct
    15601 aaatctaata catgtatata gtctctaact ccagttccat catgtgttgg atagtcatta
    15661 ccataaacac tcaattcctt caatttacct actgctattt ttgttatata aggcattaga
    15721 ttgctcggaa ctccatttgg ttcttcacct attctaccac ttttatgagc accgactgga
    15781 ttaaagtatc ttaaaagagc tatatccaat gacttatctg cttttgatat atctactaac
    15841 atctgctcta tcataagttt agttcttcca tatggattgg ttacagaaag tggaaaatcc
    15901 tctaatatag gacaagtatg tgggtctcca tatacagtag ctgaagaact aaatacaaac
    15961 tttttaacac catattctct cattaattca aataaattta aggtacttat tagattattg
    16021 ctgtaatatt caattggttt ttctactgat tcaccaactg cttttaaagc tgcaaagtgt
    16081 ataattgatt caatattatt ttctttaaat acaacatgca tttcatcttt atttctgata
    16141 tcaatattat aaaattttac tggtttttta gataattcct tgattctatc aaccactatt
    16201 gagttgctat tacttaagtt atcaactata acaacttcat aacctgattc taaaagttct
    16261 attgctgtat ggcttcctat atatcctgct cctccagcta ctaaaactgc cattgtaatt
    16321 acctcttttc taatatttat ttttcactta ctttttttct catatagtcc attaaatcat
    16381 cttttaaatt ttctctcttt agagcaaagt ctattgttgc ctctaaaaaa ccaattttat
    16441 ctcccacatc atatcttcta ccttcaaagt tataagcata aatagcctct tgttttccaa
    16501 gagtttgaag tgcatctgtc agttgtatct ctccaccttt accaggtgtc tgtttctcta
    16561 atatgctaaa tattgcagga gttattatat atcttccaag tatagctata tcagatggag
    16621 cttcttcaat tgcaggtttt tcaaccatat ctttaacttt atatactcta tcttcgatat
    16681 gttttacatc caatatacca tatttattag tatcttcttt agctatcttt tgaacaccta
    16741 atacagttgt tttatactca ttataagtat ctattaattg ttttaagcac ggaacatctg
    16801 aatctactat atcatcacct aataatacgg caaaaggttc atcacctata aaactttttg
    16861 cacagtaaat tgcatgtcca agaccttttg gctctttttg tcttatataa tgaatattta
    16921 ccatatttga tatgtctctt accatttcta acatctctgt cttacctttt tgctctagtt
    16981 ctaactctaa ttcaactgat ttatcaaaat gatcttctat agatttttta tttctacctg
    17041 ttattataag aatttcttca attcccgatt ctatagcttc ttctattata tactgtaaag
    17101 ttggtttatc tactatagga agcatttctt tgggctgtga ttttgttgct ggtaaaaatc
    17161 tagttcctag ccctgctgct ggtattattg cttttttaac tgtcatctgc attttttcac
    17221 ccttcttcgt aaattttcta ttgtaccaaa ttaatatacc atatttatac tttttcttca
    17281 aactctatat tatcccaata tttaatgcta taagataata taaggtagat gaaatttcat
    17341 ctacctttag ttttataagt taatattgta ttgtttatat tttatccaaa acttatacag
    17401 gtactaaatt tcatctaaga tgccttttat atcactatct atatcttcat ctagataaga
    17461 atctaaatct tctaagtcaa cttctagttc ttgatatgtc atatcaacac tgacatcctc
    17521 atctatatcc atttctgata ttaattctcc taaaatacta tttccatcag aatctaaacc
    17581 actatctata tcttcatcta tatagttttc atttaaaatt tcatctaaat caatctcgta
    17641 ataaacttca ttattttttt catcccctga gtccaatcct aagaactcat agttaaaagt
    17701 ccaaacattg tatttacttg acatacttta ttcctcctgt gaaaaacata ttttcatcta
    17761 gtttattggt tccataatca acagagttat atattttttc aattatgtct tttcttcttt
    17821 cttttgtact attatttaat gtcaattgtt taaatacata tgtatttgtt attctctcga
    17881 ataatatttc ttttatacta tcatcatcaa tatttctata gtttttccac aataactcaa
    17941 ataacttaaa catttcccta ctagtttcat ctacataata aggatacata ttttctacta
    18001 aatggtttaa tcttgatatg gcttgtttta aaaatacatc aagacttgca ctttcacttt
    18061 cttttaacat tctataattg tatttcttct tatcttctcc tgaaattaca aagatgatat
    18121 tgtctaaact tttttcaact tcatttaaat aactgcttat attatatgat aaatcagaca
    18181 atagattttt aatctgctta aacacttgta catcaagttt agtattatac ttttcaaaca
    18241 aatctatttc ttgattttct aatattcttg gcatatatgc tgacaataaa tctaaattgt
    18301 ttctttgttc ttgtatttgc ttataaagtg aatttataac ttctattctt atttcatcat
    18361 ttaactcttt ttctaaaatc ttatcaaatt cagatttcat atagtaaata tttccaaaga
    18421 aattaaatat tatcatatca ttcatagctt tagaccaatt cttatatatc acttcaatag
    18481 aattttcttt aagtagaggt tgctttgcat atataaatac atcttgcata agtttttgaa
    18541 ccatactttt acttaagttt aaaagtactt tttgattatt aaattttatt tccgtttcta
    18601 caaaattaga attttcgttt tcaagttctt tattaattac aatgtctttt atatcactat
    18661 tttttataga tatgatttct ttttgaaaag actttagaat agtcaagcta tcgaaatctt
    18721 cttgaataaa aaatggagca tgttttaatg tatatcccac tatttcattt tctatatctt
    18781 ttaggtcatc tatttgcata atttctctat ttttatcatt tatatgtagg ttaaatacat
    18841 ttttattatc atataatatt atatcttctc ctaaatttaa cttagaacaa ttatctttcg
    18901 ctgactttac aatagtcttc ttagatttct tatttataaa aattaattgg tcttgatatt
    18961 ttactaaaag catgtttgtg tttttataat caaatccatc tatatttcct gatatcttgc
    19021 agaatactat ttcacaatct ttaggaagat ttcctatttt ttggttatct ttaactctct
    19081 catttaaatc agatgggact acactatcta tattgttatt aaatatagat acagacctta
    19141 ctataatccc ctctctttcc atatgaaagt atcccttcaa tgacactctt ttatcttcta
    19201 aaatgtatct ttctatatca tcaaaattta tttcaaatct atttttccca ctgataaata
    19261 taaacttctc attatcttta ttaattccac ccatataaaa cttattgtca tctaaactta
    19321 atatagtcat attatccttt agatagaata taaattctct ttgacttttg cagattaaat
    19381 catattcatc caaagttatt ttcatagtca aatttgctgt aacattttta cttgaatttt
    19441 cactttttat gtaaaaaggc aagttatttc catagacaat tatttgagaa tcttctaaaa
    19501 ctgcctctaa tttttcttta ttgtctgaaa aactatatat atattttaca cctgtatcct
    19561 cattaacttc atctgatttg ctgattatta tattccctag atttgttata tgaagctttt
    19621 tatattcttt tgagtatcta cttatgccac taagctcaaa cccatctgaa agctcatata
    19681 tgattttttc tcctaatttc aacatacctt caccacctaa acagaattat aagtatttta
    19741 taaaggtgaa taaaatgtct caaaacaaat aatatttttt ttaatacttt ttattctgag
    19801 acatagtttc tcctttttta taatgctcta agcaccttca actatttctt cataaagttt
    19861 atctaaatgt ttaacatatt cacgatatcc agatttgttc tttaactcac ttcttaaatc
    19921 ttcatatttt tgtttaagtt cttcagcact caaagaagga gctgattctt ttaattcttc
    19981 taacttataa tcatagtact tatcatacat agctatatat gtatcaatag acttcttagc
    20041 tagttcatta catttttctt gtgcttctcc atattgagaa taatcaccta tttttatttt
    20101 atagtatcca ttttcttgaa ttattgttga agaaatacct ttagcttcat attcttttgc
    20161 tgtttctttt gctgtttttt caactttaag tgaacctgca taaacagtgt aatatacacc
    20221 tttaccatct ttatatgctt ccatttcttt tgctttcttt tcagcttgtg ctttttcttg
    20281 agcctctttt tcagcttttg ccttttcttc tgcttctttt ttctcttttt cagcttgtgc
    20341 ttttttctct tcttcagtta atttattgtt agcttgttgt tgagttgatg actttggaat
    20401 attctcttta tcatttttct tattgttgcc tactgttata aaatatccta caccaacaat
    20461 aaccactata actactgcta taatagtttc ttttaatcta ctcttcttag aaacaacctc
    20521 ttcatcatct aaagaatctt ctttatctga tacataattt ttcatttctt tcttgttttc
    20581 tctatatttg tccttagaag aatttttaga ttttttatta ctattttctt tctttttaat
    20641 aggtattact actgattttt cattttctaa tttttcttct tttatttctt ttgctttttt
    20701 atcttctaag tttttacttt ctttttcaag cttcgattca ttatttgaag atactttttc
    20761 atctaagtta ttatttattg gtttttctat ttttacacta cgttcttctt ctatatgttg
    20821 acttggttct tcttgtttta tttctttcaa ttcattttgc tctatttctt ctgatttcac
    20881 ttgatgattt tcaactggtt tatctaaaat tatatctgga gaaatatcat ttaaattctc
    20941 ttcttcttgt tcagcttctt cattgtcttt tttaataaat ccaaataatt tagccttctt
    21001 tttactcttt ttattttctg gttcaattat atcttcttta ttgctgtcgt actctttatc
    21061 tgtagtatct ttatcttcta caacgtcacc tatatcttga ttattactat ctacttcttc
    21121 tttagtccct tcttcagcat catcttctat tgaaatacta cttctaaagc aaatatcttt
    21181 atctgcattt tttatcatgt aaatgtattc ttcaatatcg atatctttat ttcttctagt
    21241 tctttttgat gcaatttcat gattttctaa ttcactatag ttatctttta ttagattctg
    21301 tgctttattc catgtatctt cactaatttc tgttttattt ttaattacat agtccacaaa
    21361 ttccttaaaa ttttcaggac caaattctcc aattatagtc agtctcattg caccttctat
    21421 aaggtctcta tttcttctct ttaaaaattc taatacaact gttgcacaag caaaatcttt
    21481 aataatttct ttgccaaaac catatgcttc aacagaatga agttcttcta atccacataa
    21541 cttcttatat tcattgtgta ttgtttgaga tatactttca ttatttaagc tatttaactt
    21601 atctataaac ttcgattcta tatcatttga ttttgattca tgttttactt ttgaattttt
    21661 aatttcattt agtagcatgt cattttctga attatcttta cctaattgca aatcctcaat
    21721 ttcagttgct tttatttcta ctttgttttg ttcgttattc tcttccacat tattaggtac
    21781 cacatcttta ttactagcta atattttgtt tacacagaat ataatttctc tttttaattc
    21841 tcttttcgca tctgcttgat ttttaagaaa taaatctcca cattctttct tttctaaact
    21901 tatttttcta agagatatat taagaaggtc tttatatagg tttaaaacct tataatcctt
    21961 ttctaaacgt cttcttctct ctgatagttc cattttatca taagctaaaa aactatttaa
    22021 atcattggct tttagagagt ctaatccctc tttcatagcc ttatttacat actttctaca
    22081 ttttgctgta gttaactcta aatctttttc taattcatct atttcaggta tgtacttatc
    22141 tactatccat ttgccaataa gtggttctgt atttcttgct aatgtcttat aaaacaatat
    22201 attaaatttt aaaagctctg catattttgc ttcattaaat tctacttttt caaatttctt
    22261 ttccaaaaca ccatctagct tttcatttat tttagactct tgttttaaaa aacttgctgt
    22321 gacatttaaa acagtgtttc ttattatatt attttcactc aaaaccttca ctccttaaat
    22381 caattatttt attgtaatat ttacattgtg caaagttgct aatttgatta tgatatctaa
    22441 ataattattt actaaatttg ctacatctta tatcatatca tattatttac atcattttac
    22501 tttttctttt tatatctatc ttatattata ccatttttaa tatctgtact aacttatatt
    22561 acaatattcc tcaaaatttt aaaagtagat tagacaaatt tacaaataaa aaaacatcag
    22621 tttaaactga tgtttttgac tatcgaaata attaagcaag tctataagcg gagttctgta
    22681 ttagataatc atctttctag gcctattgtc accaataggc tcaagcgacc tacctaccgg
    22741 agagtacgag cagcactctt tattcctatc ttggtcttgc tccaggtggg gtttacatag
    22801 ccaactagtc gcctagctgc tggtgagctc ttacctcgcc tttccatcct taccacaata
    22861 gtggcggttt atttctgttg cactatcctt aggatcgctc ccactaggcg ttacctagca
    22921 ccctgctcta tggagctccg actttcctca tgtaagatta catgcgatta tctggcttac
    22981 ttaatattta ttttacttag ttatgttaac acaaattctt ttacatgtca aagtaatatt
    23041 atactaaaaa tttaaattta ttcaaaaata gtgtaatata gatatataaa ctacatttaa
    23101 ctcgaggtga tctattgaaa aaattatata gaatagtaat taacattata ctagtattag
    23161 taatacttta cagtggattt aatatatact caaaacttac caaatacaat catgatacta
    23221 aaatatcctc agaacttcaa aaaaaagaat ataaaaaaga ggatttatca aagattaaca
    23281 gtgattttaa attttggctt tctgttgaga atacaaatat aaattatcca gttgtacaat
    23341 ctaaagataa ttcttactat cttgacaaag atttttataa aaaagattct atttctggta
    23401 cactgtttat ggattataga aataaatcta ttgatgacaa aaatatcata atatatggac
    23461 ataatatgaa aaataaaact atgttcaaca acctaaataa gtttaaagat gctgacttct
    23521 ttaaaaaaaa taataaaata aaaataacct tgaatggaaa agaattttta tatgatgttt
    23581 tttctgctta tattgtagaa tctgattatg attatctaaa aacaaatttt aacaatgaat
    23641 ctgattatca aaattacata aatgatatta cttctaaatc attgtataaa tctcctatta
    23701 aggtaaatag caatgataaa atagttacac tttctacatg tacctatgaa tttgatgatg
    23761 ccagaatggt gattcatggt agattgattt aaaactcaca gacacagttt aaaacaataa
    23821 ttttaaactg tgtcatcttt tatacctata atttgtaaat tagaccacta aaagtattta
    23881 atacaaaata acaaatacat caaacaataa aaaacaataa aaaaagacta tgctaaaaac
    23941 atagtcttta ctggagctgg tgataggaat cgaacctaca acctgctgat tacaagtcag
    24001 ctgctctacc gttgagccac accagcacac ctattttatt gtaaattagc aaaactaatt
    24061 tttaaaagtt a
//
LOCUS       DAMJYX010000052        22948 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJYX010000052 DAMJYX010000000
VERSION     DAMJYX010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.052"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            95..931
                     /locus_tag="PC479_002164"
     CDS             95..931
                     /locus_tag="PC479_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2804705.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1284..2861
                     /locus_tag="PC479_002165"
     CDS             1284..2861
                     /locus_tag="PC479_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2804706.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2854..4038
                     /locus_tag="PC479_002166"
     CDS             2854..4038
                     /locus_tag="PC479_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2804707.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            4056..4655
                     /locus_tag="PC479_002167"
     CDS             4056..4655
                     /locus_tag="PC479_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2804708.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4679..5149
                     /locus_tag="PC479_002168"
     CDS             4679..5149
                     /locus_tag="PC479_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2804709.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5508..6254
                     /locus_tag="PC479_002169"
     CDS             5508..6254
                     /locus_tag="PC479_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2804710.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6315..6989
                     /locus_tag="PC479_002170"
     CDS             6315..6989
                     /locus_tag="PC479_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2804711.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            7357..8703
                     /locus_tag="PC479_002171"
     CDS             7357..8703
                     /locus_tag="PC479_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2804712.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            8730..10130
                     /locus_tag="PC479_002172"
     CDS             8730..10130
                     /locus_tag="PC479_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2804713.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            10340..11776
                     /locus_tag="PC479_002173"
     CDS             10340..11776
                     /locus_tag="PC479_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2804714.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            12120..13064
                     /locus_tag="PC479_002174"
     CDS             12120..13064
                     /locus_tag="PC479_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2804715.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13233..14165
                     /locus_tag="PC479_002175"
     CDS             13233..14165
                     /locus_tag="PC479_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2804716.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14270..14737
                     /locus_tag="PC479_002176"
     CDS             14270..14737
                     /locus_tag="PC479_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2804717.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(14981..15835)
                     /locus_tag="PC479_002177"
     CDS             complement(14981..15835)
                     /locus_tag="PC479_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2804718.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            16001..17965
                     /locus_tag="PC479_002178"
     CDS             16001..17965
                     /locus_tag="PC479_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2804719.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            17965..18426
                     /locus_tag="PC479_002179"
     CDS             17965..18426
                     /locus_tag="PC479_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2804720.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18429..18749
                     /locus_tag="PC479_002180"
     CDS             18429..18749
                     /locus_tag="PC479_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2804721.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18765..19865
                     /locus_tag="PC479_002181"
     CDS             18765..19865
                     /locus_tag="PC479_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2804722.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            19985..22585
                     /locus_tag="PC479_002182"
     CDS             19985..22585
                     /locus_tag="PC479_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2804723.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         9013 a         2357 c         4054 g         7524 t
ORIGIN      
        1 tatatacgaa agtaaaattt taaatattga cattaaatta aatatactat actatgtaag
       61 tgtgatgaga gaattttttt agaggggatt tatagtgaat ataaaaaaga tatttaataa
      121 caatgtagta gtaagctccc tagaagatgg cacagaaata attgtcactg gtgctggagt
      181 aggatttaag aaaaaagtag gcgatttgat agatgaaaat ttgatttcta agaaatattt
      241 tgttcaagac gatcaaaggg acaaatacaa tcaaatactt aataagacct ctattgaata
      301 ctttaaaatt tcagaagaaa ttatagaaaa ggctaatgaa gttttaaata ctcaagtgaa
      361 tgattcaata atattagcct taactagtca catagaattt gctgtccaaa gagaaaaaca
      421 aggtataaaa ttaccaaact taatattgaa tgagacaaaa caactttata gggaagaatt
      481 tgaatttgga ttatgggcaa ttgatgaaat tgaaaaaagg ataggaataa aattgcctga
      541 agatgaagca ggatatattg ctattcacat aatcaatggt ttggaaaatt ctcaaaaaga
      601 tgaaggtatt aatatactag agttttcaaa acaagttatt cagataattg aagaagtaca
      661 tggatttaaa ttggatgtaa attcattaaa ttatactaga cttataactc acttaaagtt
      721 ttttgttcaa aggattctcc gaaaagaaac ttacaatgat agtgatattg aagatatgta
      781 taagttagtg aataaaaatt acaataaacc taaagtgtgt actgaagaaa ttgcagcatt
      841 ggtattagac agatttgaat ataagattag taaagaagaa gaattatatt taatgattca
      901 cataaataaa ataacaaaca gtggttgtta ggattgtaac tgataaagca ggcaaaacct
      961 aagtcattga gcttaactat tagattaagt tttttgattt aggttttttt attagataca
     1021 atcaaaatac agacagtgac ttttaggatt gtaactggtt aagcaggcaa aacctaaatc
     1081 attgaattta actattaggt taagtttttt gatttaggtt tttttattag atacaatcaa
     1141 atacagacag tggttgttag gattgtaact ggttgagcag gcaaaaccta aatcattaaa
     1201 cttaattatt aaattaagtt ttttgattta ggtttttcta taaatttgat tatgttttta
     1261 gataaaaata ggaggggaaa attatgaaaa aaaataagtt aggtttatgg aacttcttcc
     1321 aaatgttagg aaaaacgttt atgttcccaa tagcactact tagtgtttct gggatgatgc
     1381 taggacttgg ggcaggtttt acagacccta agatgattga aatggttcca tttttaggga
     1441 atgaatatgt taacttagtt ttaaacttca tgttaacaat aggattattt gcatttaaca
     1501 acttgggagc attatttgca atggcaattc cactaggatt attaaaaaaa gaaaaagagt
     1561 ttggtgcttt ttcaggatta gtaggattta tagcaatgca tataggtaca aatttttatt
     1621 taacaagagc tgatttgtta gtaccagcag aacagatgtc aactaatggt caagctatga
     1681 ttatgggtat acaaacatat aatacaagtg ttttaggtgg tattgtagca ggattaattg
     1741 tttatgcaat atatgataaa gttagtaatt taaagatacc agaatcatta ggattctaca
     1801 gtggaccaag attagttcca attgttagtt taatagttat gagtatagtt ggattattaa
     1861 tacctattgt ttggccacca tttttcaatg cattccaaag tttaggaaga tggatttctt
     1921 cagcaggacc attaggatat ttttcatatg cagtggcaga aagagttaca attccatttg
     1981 gactaaatca tcttgtaaca tctgtattcc gttttacacc aataggtgga actgctgtta
     2041 ttgatggaga aacgtacttt ggtacattga acatgtttat ggcatatgta gaaaataatc
     2101 agattatacc tcttgattta gctggtaaaa tggaacaagg taaattgatg attcaatatg
     2161 gattagctgg tgctgcattg gctatctacc gtacagcaaa accagaaaat agaaaggcac
     2221 ttaaaggact tttaatatca ggagtgttga cagttattat aggtggtata agtgaaccaa
     2281 tagagtttct attcttattc atatcaccag cattatttgc tttccataca tttatgaatg
     2341 gtctagcaaa catggtactt ccatacttag gcgtgttgat gggattcaca ggagatttaa
     2401 ttgcatttat aagttttggt gtgttaagag gaacagctac aggatggcca atagcagtat
     2461 tagtagcagt tttatatttt gcaatatact attttgtatt taagtgggca attattaagt
     2521 ttaatataaa gaccccagga cgtgaggata aagaaataaa agtatctgaa ggaaatggtt
     2581 caaatttcaa aaatttgtct acatataaag gacaacaaat gatatctgct ttaggtggtc
     2641 aatctaatat attatcacta gataattgtg taactagatt acgtttaaaa ttacaagatg
     2701 tatcattaat aaatgaggat gcaattaaag aggctggtgg tatagcagtt gttaaattgg
     2761 atgagcatac aatacaagta attataggaa cccaagtgta ttctttaaga aagcagatgg
     2821 ataaagctat ggaaggtcat ttggaggcat gtaatgatta attttgataa aaaagtagac
     2881 cgattgggta catattgtac ccagtgggac tatgtagaag atagatttgg taagaaggat
     2941 ttgcttcctt ttacaatttc agatatggat ttagagtttc catttgaaat aacagaggtg
     3001 ttgcaggaaa ggttaaatca tagagttttg ggatacagta gatggaaaca tgatgatttt
     3061 agaaattcag ttacgaattg gtatattaaa agatttaatt gtagcgttga tagtaactgg
     3121 atatattata gtccaagtgt aatgtattca attagcaaac ttattgagat gttaagtaat
     3181 gaaggagatg gaatccttat aaatactcct gcatacaatg ctttttatga tgtgataata
     3241 aataacaatc gtaatattat aaagtcacat cttgtaaata ggaatggatt ctattcaatt
     3301 gattttaatg attttgagaa aaagtgtaaa aaatctaaga tatttatttt atgtaatcct
     3361 cacaatccaa ctggtagagt atggagtgag ttagaacttg ttaagatgat aggaatttgt
     3421 aagaataatg atgtcaagat aatatcagat gatatacaca tggatattgt atatgacaat
     3481 agaatgacac ctatcttaaa tgttgcaaag gactatttag attctgtatt catttgtaca
     3541 tcagcgtcta aatctttcaa tataccagca ttgactgggt catatgtgat tataccaaat
     3601 gaaggtatta gaaataaatt tgaaaatatc actagatacc gtgattttgt aaattcgcca
     3661 gcaattctag caatattagc cacaatggtc tgttatgatg actgcgaata ttggttagat
     3721 gaactattaa aataccttaa agagaatctt aaatatacga ttgattatat aaaagataat
     3781 ttagaaccat tgaaattggt catgccagaa ggctgttact ttgcttggat agatttttcc
     3841 gaacttaaaa taagtggtga gaaattccaa aaattattga tggatattgg agaagttgct
     3901 attatgtcgg ggtctgtata tggtgaagaa ggtgagtttt atttaaggtt aaatgtggca
     3961 tgttcaagag aaaaacttga agatggttta aataggatta aaaaaactat ggattatatt
     4021 gaaaaaagaa ttttatagta ggaagggaga tagttatggg atatttaaaa acaatggttg
     4081 atgttatgac tactgaaatg aatgccataa gatgtttaat agatgaagca ggaatagaat
     4141 atgaaagtat agttagtgaa attgcaaatt gtgaaggcaa agtaattttt atgggtgtag
     4201 gaaaatctgc acatataggt aagaaattgg cagctacatt tgctagtact ggtacaccta
     4261 gcttttttgt acatgcaaca gaggcagtgc atggagattt aggaatgatt gaaagtaagg
     4321 atattacaat tttaatatca aatagtggaa attctatgga agtggtaaac tgtataaaat
     4381 atattaaagc aataggttct aagacaattg catttacttc aaacagaaat tcagtactgg
     4441 caaaagagtg tgattatgct ttaatatatc ctgcaaagga tgaagcagac catttaaatc
     4501 tagcaccaac aacatcatct acaattactt tggttttagg agattctatt gcatgtgcat
     4561 tatctaagag tagtaatttt ggttcttcag atttttataa gtaccatcca ggaggaagtt
     4621 taggtgagaa gttaaaaaca gctaacaatg gataaaagca aaaaggagag tagtttttat
     4681 gttcaaaatg tttaaaaaaa aatcaataaa atcacccata tcaggaaaag taatagagtt
     4741 atctaaaata caagatggtg tattttctca aaaaattatg ggagagggtg tagctataga
     4801 ttctacagga gacattgtat atgctccaat caatggaaag atagtagtag tagcagagac
     4861 taaacatgca tttgtaatag aattagaaaa tggaatggaa cttcttattc atgttggttt
     4921 agatactgta aatttaaaag gcgaaggttt tgaggcattg gtgagtatgg gagaacaagt
     4981 gaaggaagga acaccaatgt tgaaaattaa ccgtcctttg attgaatcaa agggtatatc
     5041 acttattact ccagttacaa taactaatca ttcagagtac aatatgaata catgtaatgt
     5101 aggcaatagt gttgaaggtg ggaaagatac tgtaattgag tttaaataaa gaactaaaat
     5161 acaaggttaa tatttataaa tacctataaa cttatgtttt ataagattga ttgtatagat
     5221 ttgttgtata aaaaagaact ctttaattat aaattaagga gttctttttt attttgtaaa
     5281 tattgtttag atttatatag aaaaacactt agtaataaat agaataaata agtatttata
     5341 tagctaaaaa tctaaatagt tttgtttaaa tatattttta tattttgtaa tttcaaatta
     5401 ataaataatt agatttgata aacaaaaaat aaaaatgttg attatattaa taaactttaa
     5461 tataatatat ttatgaaaaa tgtaaactgt gaaatggagg tagtaacatg aaaattgaaa
     5521 atctttctat tgatgatatt aaacgaggct atatatttaa tgatgattta aattgttata
     5581 gttgcattaa atgtacaaaa acatatgagg taggagaaat atataaaata ggtaatagat
     5641 tctttgaagc ttcaaaagct attgaacttc atatggaatt agaacatagt gattatttag
     5701 agcaattgct taatagtgat tcaaaataca acacattgac tgaaaatcaa aaacaattat
     5761 ttcaattatt tattagtgat ttaccagata aggagatagc aaaacaggtt ggagtatcca
     5821 catctactat acgccatcaa aaatttattt tcagagaaaa agcaaaacag gctaagctct
     5881 atttagcact atatgaatct gtatttgaag ataaatctaa taaaagtagt actataatac
     5941 caatccatga acatgctcga atggtagatg aaagatatgt agtaactgaa gaagagagaa
     6001 aacacatttt ggatacatca tttgaaagta tgaatccttt aaaattaaaa gtattttctt
     6061 caaaagaaaa gaaaaaggtc gtaattttat ctaagatttt ggaacaattt gaacgtggga
     6121 agaaatattc agaaaaggaa gttaatcaga ttttagaatc tatatatgat gattttgcaa
     6181 caattagaag atatctaatt gagtatggtt ttatgagtag aaataaagaa tgtacaaaat
     6241 attggttaac ataattaaaa taataaattt tactaatgtg taatgttatt tttatctagg
     6301 gggaggagtc aaatatgaat gatgaattaa aatctatttt atacaataaa ggtgtggaca
     6361 ttgttcgctt tgttgatata tctgaatttc caataaatca aacgcaaggt ttttctaaag
     6421 caattttatt ttgtataggg ttatcaaaaa aatttattac agatatatac aataacttac
     6481 ctacagatag tgatgagttt ttagaaaaag aggagaaagc agaagaatta gctgactgga
     6541 tttcagaata tattcaaaat aaaggatata gagcgtattc tcaatcggag aaaaacaatc
     6601 tagaacatgg atattttgaa aaagcctata ttaatccaga aatgcaatca gggattagtc
     6661 ctttaccaca taagacaata gcaaatatta gtggcattgg atttatggga aaaaataatt
     6721 tgtttgtaac agaggaatac ggatgtgctt ttagcatgtg tacagtactt acagatgctc
     6781 caatttctgt tgaaagatat cctttgattg attcaaaatg tatggattgt aatgtttgtg
     6841 tagaaaactg ccctgcaaaa gctatacatg gaaatgagtg gactttgcca ggaaaaagag
     6901 agtctattat tgatgtttca aagtgttttt gtgtattgaa gtgtatgatg agttgcccat
     6961 ggtcattaag atatgcaaat caaaaataat tttactagag aaggtattaa attgaatatt
     7021 ataaaatttt taaaatgtaa gattatataa gatttttcaa aataaaaatt tgtatagaac
     7081 actcagaaat gagtgttttt ttattggaat taaattatat tgatacaaag tttaactatt
     7141 ttaatcatta tataaaattt gtattaaaaa attaagaata acttggtaat agtgaaaata
     7201 taatgagatt ataattgatt tataatataa ctataatgat gttgtaaaat gaaaattgtc
     7261 atttcaaaat tcccaaaaag agtaaaagtc acttcggtta tagtcaaaaa attttttcag
     7321 tgaaaagaat ttagatttaa aagggaggaa ttaatcatgg aaattgaaac taaaactccg
     7381 gattggatta atgtactaga attaaacagt aaaatagata ttactgggaa atatgatgta
     7441 agtaatatta tacaagatat tgtctctgat aagtcactag aaggttccat tatatatttt
     7501 ccaaaaggta attatttatt tgagaaaggt attaaaatta gtcaacaaat aacacttcaa
     7561 ggagattctt attatggagg agataatcaa gtatcaaatt tagataaaaa acaatcaatc
     7621 ataggaacaa ccaattttat aactcgagga gtttctaata tgagtattat tgcattgaca
     7681 ggaacaagtc aatgtatcaa aaatattaat ttttattatg atagtcatga tatagagaaa
     7741 ataccgccta aaaatgtatc tgctattaca gaatatggag aaacacaagg actatctcat
     7801 tttgaacacc tgtttatttc tggattttca gggataggga ttgaaattcc atactattca
     7861 actggcaatg atataattgt tagttcatgt ggtcttggta tgaggcttgg agagaagtca
     7921 atgctttcta gtagcaagat ttacgaatgt aaaaatggaa tggagattac tactggtgtt
     7981 agcttaaata atgtgagaat agaagaaata aaagaaatag gtattaataa taaaggattt
     8041 ggttttaacc tgattatgaa tcttatggtc gaccaatgtg gatattgtgg atttatgttt
     8101 gaaaaaatgt gctatagcca gataactgcc agaattacta gatgcggtca gtattataaa
     8161 agtgttgatt ataatgctta tgagaatatg gaagatagaa aagaaaaagc ttattccatt
     8221 ttttatggag atattttaga aagttgcaat attgtattga tgaatagaaa tattgatacc
     8281 ttagaaagtg attcacttaa tgaacataag atatatgtaa tcaaggcaaa tgaaacaaac
     8341 aatgtaatgc taacatgtta tgctgaagca gataacttta tacaatgtgc aaagggcaac
     8401 ttgctgttag aaaatggtga aaatacttat aaattttata atggtgagat ttgttctgta
     8461 ggaggtgttc aaatttctga tatagatgaa aaagatttaa tcaaaataat agagaacact
     8521 ttgtatatag atgataaagg taagtattta atggttccta gattaaaaaa gaatactgta
     8581 atatattcag tactagattc tacagaagaa ataaataaat tacatggtgg aatatgggaa
     8641 cagattgatg taaaagtatt agctggggaa tccatgtact attataaaaa gataaaagat
     8701 taaattatca aactaggagg tagtgattaa tggaagagtc tataataatt aaagatataa
     8761 gtggctggat taatgtactt gattatggaa tttgtaatga tgaaacaaaa gatgtaagtt
     8821 ttaagttgca agagattatg gatgctgtac cagaagattc tattatatat tttccaaaag
     8881 gaaaatactt atttgaaagt ggtgttaaga ttaagaaacg attaacctta tgtggagatt
     8941 cttatatttt agcgaatgtt cctaaaaata ctgctggagt aacacagttt acttttaaag
     9001 gaaagaaaaa tcctaaaagt gaaaatagag caatcataac agcaaaaagt gtaagacact
     9061 gtattaagaa catttgcttt tattcagata gttgtgatag agatatgaca tctaatcatg
     9121 aacctccaac agatggaaaa cctagatttc atcataatgt aattataaat tatagaaata
     9181 tatctgctgt tatatgtgaa gataaacagg gagaaccagg tcattatgaa aatctatctt
     9241 ttagtggttt ttctggaatt gcacttgata tgcctaataa ttctacagca aatgatatta
     9301 caatttttac atccggactt ggaattagta caggggaaaa ttcaacaatt gctaacagta
     9361 aggtttgggg ctgtgttgat ggaatgataa tttctactgg aacttttata aatggtatga
     9421 gggtagaaga aataaaaaaa gttggaatta aaaatattgg tagaggttta aattttatta
     9481 caaatatcac aattgaccaa tgtggatatt gtggagtttg gtttgattct atatccaatg
     9541 ttcagatatc aggaaatatc acaaggtgtg ggcagtacta ttatgatgta agctatgata
     9601 attatttaat gttggataat agagttaaag aagcatattc tttattctat ggtaatttat
     9661 tggagtcaag taatatagtt ttatcaaata ccaatgaaga taattgggaa gatttcaaag
     9721 aagataagca taaggtgtat cttatagaag cacttaaaac caagaatgta ctattaacat
     9781 gcaatataga tgctactgac tggattgtta agtcagaaaa aggaaattta acgtacaata
     9841 atatgcgaca aactgttata ttttacgatg gaaaactaag tagtatcaat ggaattggta
     9901 tatctgatga agattatggg gataaagtca agataaaaaa aggaaatata tatataaata
     9961 gtaatgataa ggatattaag atgcataaac cagaggtagg tgacattatg tcaacaatta
    10021 ttaaagataa aagtattctt tctgctcatt atgggggaac atgggagaaa attggtgaaa
    10081 aaatagagtt tggaataact gtacactatt ataaattgat agagaaataa gtaaatacgt
    10141 agtatttgaa tagatgtatt ttattggaga gttaggacta tagttttaat ataaatggtt
    10201 tggtaatagc atggattgaa taatataaaa ataatataaa aaattaattt gattatgtaa
    10261 atttctatga agtagtaaaa ctgtgacttt agtcatagga ggttcaaacc aagtaaaagg
    10321 gaaaggaata tataatatta tggaaggaag aactgggatt aaagatggaa gatggattaa
    10381 tgtagtagaa agaggtatta aaaatgatgg ttcgtacaac ataggtcctg ctttgcagga
    10441 tattattgat gaaatagaaa cagatttttg tgtcttatat tttccaaaag gtaaatacct
    10501 atttacaagt ggtgttaaaa ttaataaaga aattgtattg caaggtgatt ctaattcatc
    10561 tacaggtact acacaattta taactcgtgg agtagaaaat atgagtatta ttacattgac
    10621 agggaagaaa caatgtatta aaaatataaa tttctattct gatagttgtg agatacaggt
    10681 aaataaagaa ccaccgacaa aaggtaatcc taaatatcat tatgagatga ttattaataa
    10741 gaatgaaaag aatgttgaga tgaataatgt atcagcaatc ctgtatgata acagtaagaa
    10801 tatgattaaa ggtctaggtc attatgaaaa tatttatata agtggttttt cgggtacagg
    10861 aattaggata ccatattact caattgtaaa tgatattaca gtttcatctt gtgggctggg
    10921 tattgataca ggaatagaca caattatttc taatagtaga atatctaagt gtcaaaatgg
    10981 aatgaggatt agaactggaa ctagtataaa taatgtaagg attgaaaaaa tacagaaagt
    11041 tggtcttata agtagtcaag ttaaggggta tgaggggtat ggaagttata aaattaataa
    11101 tattacgatt gaccaatgtg ggtattgtgg cttttcattt gattcaatga aagatagtta
    11161 tttttctggt attataagaa ggtgtggtca gtattattat aatacagatt atgacacata
    11221 tttaagtatt aaagatagag tagaagaagc atattcttta ttttatggaa attattttac
    11281 agaatgtaat attgatttag ttagtgataa taaggataac tgggatgatg gcttagaata
    11341 taaacataaa gtttatgtat ttaaaaccct tcaaatacca aaattaacat tgagatgcaa
    11401 tatagatgcc tatgattata tcgttaaatc ggtacatgga ggaggtaatt taatgttaaa
    11461 aaataacttt aacaattata tatttattga ttcatctaat ccagaagtta ttaatggaat
    11521 taattttaat tatcaaaaga atggaaactt gttaaggtta aataatggta aattaagtta
    11581 taaaaatgga aaggaagaaa cagctatatt cagacctaat tatggtctta ttgtagcatc
    11641 aacagattca gaatgtgaac aaatagaggc tacctatgga gaaaaatggg agaagcttgg
    11701 ttctgaagta attggagaag aaacggtata ttattataaa catcatgaac cagataaaac
    11761 aaaccaaata caataaaatt tgtattatat aatgatataa agttttaata tatagtgttt
    11821 acctttaatg taatattggt atatttgtat agtattaaat ttgttaaaaa gtaaaattgt
    11881 agggtgcttg aaaatagcac cttttttagt ttcattataa aattagtttt gaaagtttag
    11941 gaataatgta tatttatttt taaagaatta taaccttatt aaggatattt taaatgctag
    12001 aaatgaatca gtaaatcata ctggaattat cttaaatgtt ttgattaaat taagtattta
    12061 gagctataat tatattaaaa atatattaga acataattat attactatgg aggttaaata
    12121 tgatagtaag tgtgagtaga agaactgata tacctgcttt ctatagtaag tggtttttta
    12181 acagacttga agaaggtttt gtttatgtaa ttaatccaat gaatccaaaa caggttagta
    12241 agatagagtt aaatccacat actgttgatt gttttgtatt ttggacaaag gatgtaactc
    12301 caatgttata tgatttaaat aaattaagag actataaata ttattttcac tatactataa
    12361 catcatatgg aaaagaagta gagacatgta ttttagataa aagaaaggta atagattcat
    12421 ttaaggaatt gtctagaaaa ataggcaaag aaagagtaat tttaagatac gacccgattt
    12481 tcttaagtga aaagtacaca gtagattatc atatcaaagc atttgcgagt ttatgtaatc
    12541 agcttgatgg atttacagaa aaatgtataa taagttttat cgatttgtat aagaaaacaa
    12601 aatttaatac taaagcatta catataaagc caatagaaat tacagaaata gaaacattat
    12661 caaaagagat ttctaagatt gctcataagc atgggataat acttgaaatg tgttctgaag
    12721 aatataattt aagtaaattt gggattggaa aaagtaagtg tattgatgat agattaatat
    12781 caaaaattat aggatgtgaa gtagaggtaa aaaaagatag tacacaaaga gatatttgtg
    12841 gatgtgttaa aagtgtcgat ataggacaat acaatacttg cagacattat tgtctgtatt
    12901 gttatgctaa ctttaattat gcaaaagtag aagaaaactg taggctttat aaggaagata
    12961 ataaattact catagatact ttaagagatg atgcaaaaat aagcattaga gatatgaaga
    13021 ctattaaagt agataagaat aaacaaatta gtatatttga ttaaaataaa tatactaatt
    13081 accttaatat attaagaata tagatacttt tgataacaaa attaaactta atagatattt
    13141 tctatataag aaagatggtc ataatatgtc aatttgacca tctttttgat ataattaaaa
    13201 cacaaataat ttagtaacaa aggaggcaga acttgaattt attaaaaaaa tatcctatac
    13261 ctattgcaag tttgatttta tcaattttta ctttaggtaa cttattacaa tcttatagtg
    13321 aatcattacg taatgtaatt ggatttatag gatttatttt gtatgcaatt tatatagtca
    13381 aaataatatt acttagaaag ggtgttaaag agcaattaga gaacccttta atatcaagta
    13441 catttccaac tataacaatg gcaacaatga tatttgctac atatataaaa ccattatctt
    13501 tagaattaag tattatgatt tggagcatag gaattgtggg tcatcttgtg ttaatagtct
    13561 tatttagtaa gaagttctta gttaaattct caataaaaac ggtatttcca tcttggtata
    13621 tagtatatgt tgggatagtt gcagctagtg ttacggcacc agcatttaat caacttttga
    13681 ttggcaaaat tgccttttgg ataggatttg tatcttatat agttcttata ccagtaattt
    13741 taaagagagt atggataatc aaggaaatgc ctgaaccatc cttaccaagt atagctatat
    13801 tagctgcacc agggtcacta ttattgtctg gatatattaa ttctttttca gataaaaata
    13861 atgtagtatt atacttgtta ttcatattat caattgtatt ttatattata gttttgggat
    13921 atttaccgaa attattaagg ttgccttttt atccgagttt tgctgcattt acattcccta
    13981 tggcaataag tgcaatgggt atgaaactaa tgaatggtta tttaactaaa tcaggtcatt
    14041 ttataagttg gatttcatat gcttctaagt tagaagagat aattgctaca gtgattattt
    14101 tatatgtttt agttttgttt attatatatc ttagtaaatc tgaaactaca tctaaagcta
    14161 aataagaatt attaaatatg tgtatttaat aggaatattg atataaatat atgaagataa
    14221 gataaaccat agaaacttaa tttagcttaa ttttattgga ggaaaacaaa tgaaaacaaa
    14281 agaagaatat ttaaaagaag ttagaaacat atcagaagaa tactttagaa aaggagagtt
    14341 tttttgctct gaagcagttc tccagacaat aaatgatgct ttaggccaac ctttaagtcc
    14401 agagataact aagttagcat caggatttcc gataggactt ggaaaggcac aatgtctttg
    14461 tggagcaata tctgggggag agatggcttt aggtatagta tatggaagag ttcatggtga
    14521 agctatgaat ccaaagatgt ttgagcatgc taaaggtttg catgattata ttaaaaaaga
    14581 gtatggtgca acatgttgta gagctattac aaagaaatgg gatggtgata actttatgtc
    14641 tcccgaaaga aaagagcact gtattaaaat aacaggtcaa gttacagagt gggttgcaga
    14701 taagcttata gaagatgaaa aacttaatat caaatagtgt aatattagtt ggtgaattta
    14761 taaagttaaa tttacttata gtataagata cttgatattc tcgtattgat agaaaaattt
    14821 atagcatatg tatttaatga aatatactta tatttagagt gtggtttaat ttcataaata
    14881 catatgctat tattttaagt atataagcaa atttatttaa ataaatagaa acagttaatt
    14941 tatatgtaca aaactatata tgattagtgt aaataaatac ctattttata atactcaaca
    15001 taaagacacc agacatgatt agtattatac caataaaaat agcaagccat tcttttttgt
    15061 tcttcttttc tttaaaaaat ataatccctc ctaaagtggc tactatcaca caactctgag
    15121 agatagaaaa acttattgta actcccaatg tattagtcgt tatgaacata cctaaatttg
    15181 ctattgacca caatatacca gtaattaaat tgaataaaac attttcccta tatacaatct
    15241 ctttagaaaa atacttgaca ataatagatg aagttatcaa cataccaaca gattgaggta
    15301 atataattct atatccttca attaaaaata tttgattagt tacaacatat aatgataaaa
    15361 atagagatga taataataca actccaacac attttaacat atttttatct gtagattttt
    15421 tagtttgata atttgtgaga agtataccaa gtattataca taatattgat aaagaaccaa
    15481 tcagtaacat tttagaactg gtccattcat taaacaagat tacagctaat aatgtcgtaa
    15541 aagttaattg tgaaccattt gaaattggca ttactttaga tacactagca atttgaattg
    15601 ctttaaactg cattaattgt cccaaactcc agaatatgcc agaaatcata cttatacaaa
    15661 atgaaaaaaa tgtaatttca ggatgtataa ccagagttat tatagtggaa aatataaacg
    15721 cacttatgga tgtacctaaa agctgttcat gaggagaact tttctttaga tttgcaacta
    15781 tgggcatcaa tccccatccc attacaggaa gtaaagcaat aattattgtc agcattaaac
    15841 tatttccttt catattaaat ttagacagct tttaatttat catctaaaat taaagtgttc
    15901 aatacataaa aattaccgta tttaatcagg aaatattagt agttttttag aaaaacattt
    15961 tccattaaaa taaagacaaa gggaaggagt aaaattgaaa atgttaactg atatgattca
    16021 aaatcgtgaa attaaaataa taaagttttt attaaaagaa ggtcatacaa ctgtaaaagc
    16081 aatagcagat gatttagatg tttcagaaaa aacagtttca aaatctctta aagaagtaga
    16141 agcagtgctt aaaaagatag gtctttcttt aataagaaaa cctaagatag gtgtttatat
    16201 tgatggagat ttaaaaaaaa tacttcctta cctagataat aaaggaagtc aaattcctac
    16261 aaccagagag gagagagtta tctatatata ttcagtactt ttaaagagta atgattatat
    16321 tactatacaa caactttctg atgaactata tataagtcgt ggaactattg aaagggattt
    16381 agcagaagtt gaaaaactgc taaaaaacga agggatttcc atatataaga aacctagtaa
    16441 gggaataaag ttaaatataa gtgaaagaga taaaagacaa cttactgcta agtttataca
    16501 taaattttgg agtagaaact ggtatattaa gcaagagcaa gaaagctttt atcaagcatt
    16561 tgaaaagata caagcagatg taaatggtat tttttcggaa gatgatttat caataattat
    16621 tgatattctt aggaatttca gcagagaaag aaaatttgag ttctctgatt atgaatttca
    16681 gtcattagtt attcatttgg caatagctgt agagagaatt agaaaaggcg aatatataga
    16741 agaaagtttg tatactggta atatagaaaa taatcaaatg tcaaatacca attatttagc
    16801 aaaacaatta gaagaaaaat tttcaatatt attaccaaaa tctgaaattg gatatataaa
    16861 tttacattta gtagcgtctg accaacatag agatactaat gaactaaaaa atacttcaac
    16921 taatccttgt gagacaagta caaataatat atcaaatctt cgggaaatta taaaaaatac
    16981 attgtatgag acagcttatg acaaggattt aattgatgga ttaacttcac atgtacattc
    17041 atcaataaat agactgaaat atggacttag tattaaaaat ccatatgtaa atacaatcaa
    17101 acaaaacttt tctttatcat ttgagcaagc tttagattta aagaaagtaa ttgaatttat
    17161 atatgatatt aggataaatg atgatgaatg tgcttatata gcattacatt ttgaagcttt
    17221 tagagaaaga atgaaagaac ttccacaaaa tattagagta attctagtat gcagtacagg
    17281 attaggttca tctaggttat tgtctgcaag aataaaaaaa tactttccaa tgataaagat
    17341 tgaaaaagta ctttccgtac aagaattagt atccagtaaa attgatgcag atttagtaat
    17401 aagtactatt tatttagagt tagaaacaat tccaaccata gtagttagtc ctattttaaa
    17461 tgaaagtgat ataagaacct tggaaagaaa tatacaaaat gtttcaagtt atacttctaa
    17521 gaagtataaa tctttcagaa acttaatctt tgaagaaaat atattgttga atattgaagc
    17581 ttcaaactat gaagatgcaa ttaagtatat aacagataac ctaataaaaa aaggttttgc
    17641 aaaagaaggt atagctaaaa gtgcaattga gagagaaaat ttatcttaca cttctttcca
    17701 atcaatagct acacctcatg ctaatcctag ttatattaaa aaatctaata tatctgttgc
    17761 aacgttaaaa aatcctatta catggggaaa tgaaaaggtg gaagttattt tctttatttc
    17821 attagaaaat gacaaaaacc tagagcttga tgaaatatat gaagtttttt atgacttcat
    17881 tgacaataaa aaaaatataa atgacatatt aagtgcaact tccacaaagg aagtgtacga
    17941 tttgctagtg gaggagagta tatagtgaaa atatcagatg tattgaaaaa agagcaaatt
    18001 gtttttaatc ttgatacaaa tacaaaggaa gaaacaataa aaaaattaag tagtatattt
    18061 gttgaaaatg ggattgtgga caatgaagaa gaatatgtta agtctgtttt ggaaagagaa
    18121 gaacattcaa ctacaggtat tggtaatggt attgctattc ctcatgggaa aagtaatgct
    18181 gtcaaaaaat cttctataat atttgcaaag actaagcata agattgaatg ggatgcttta
    18241 gatgaaaaac cagtagatag tattttctta ttggctataa gcaatattga taaggatagc
    18301 aatcatcttg tactattatc taaattagca actaaactta tggatgatga caatgtagat
    18361 gcactaaaat tagccaattc agaacaagaa ataatcaata tatttagtga agaaggagag
    18421 atgtagaaat gaaaagaaaa attatagcag ttacagcatg tgcaacagga gttgcacata
    18481 catatatggc agctcaagcg ttaaagaaag cttcaaaaaa gatgggtcac cttataaaag
    18541 ttgaaacaca aggagctaca ggaatcgata atgagttaac tacaaaggat gcacaaattg
    18601 cagaagtagt aatatttgca gtagatacta aagtcagaaa tgctgaacgt tttgagggaa
    18661 aagaaatact agaagtacca gttaatgcac ctataaagga tgcagagaaa gtaataaatg
    18721 atgcactaaa gttaattgac actaaataaa tggagggaaa tgtaatgttg aaaaagatag
    18781 tctctgagtt aaaaaagcat gtcttaactg gaatctcata tatgattcca ttagtaattg
    18841 caggtgctat gataatggcg atatctcgtg taggtggttc tttctataat ataccagata
    18901 tatgggatgc taaatatgca gaaagtgcta gtagtattgt tagattatta catgatttag
    18961 atgggtttgg aggtactgca ttaggattaa tgtttcctgt aattgctgca tttataggat
    19021 tttctatagc tgataaatta gctattgtac caggtcttgt tggaggtatg attgctaaag
    19081 atataggtgc aggattttta ggagctttgg cagttggtct tatagcagga tatacatgtt
    19141 tgtttattaa aaatcatgtt aaattaccaa aatcagcagc ttcaatagta ccaatattta
    19201 tagtaccagt atttgggaca ttaatcactg ttgtattaat caattatgtg ataggtatac
    19261 cttttgccac attaaatact ggattagaaa attggttaaa tggattatct ggtacaaatc
    19321 agatactaat ggcagctatt ataggggcta tgatggcagt tgacttaggt ggaccagtta
    19381 ataaagcagc tttaacaaca tcacttgcat tattaacaag tggaatttat gcgcctaaca
    19441 ctgctgcaat ggtaggaata gttataccac cactaggatt gggacttgct actatactag
    19501 caaaacaaaa atataataaa cagttacgtg aagctggaaa atcatctctt ataatgggtt
    19561 taattggagt aagtgaagga gcaattcctt ttgcggtaga atctccatta aaagtaatac
    19621 cttcatgtgt aattggaaca gcaattgctt cagctatggc agttggttta ggttcagtaa
    19681 atgcaactcc aattagtgga ttttatggat ggtttacagt agagaattgg ccaatgtatg
    19741 tgttatcaat agcagttgga acagtaattg tagctggatt agttatagta ttgcgtggaa
    19801 atccagtgat tgaggatgat gaatttgaag atgatgattt tgatgaagca gagtgggaat
    19861 cttaaaattt aataactatt taagacgtgt tataagctat attcaatatg aatcttatac
    19921 taaataaagt gttaaacatt cagtttagac ttaaatataa aagataaaga acagaggaga
    19981 ataaatgata aaagcacata ttgttaatca tacacactgg gatagagaat ggtattttac
    20041 ttctggagat gcattagtac ttagtgaaca actatttaca gatgtaattg atgaattaga
    20101 acgtaatcca gatgtaagtt ttgtacttga tggacaactt tctattttgg atgattatgt
    20161 acaattacac aaagaaaaaa tagaaatcat taaaaagtta ataaaagaag atagactaca
    20221 tattggacca tggtttactc aaactgatgc attctttgct agaggagagt ctattttaag
    20281 aaacttaatg attggaattt ttgaaagtaa aaagtatggt aagtatatga aaattgggta
    20341 tttgccagat acatttggat ttaatgcaca gatgcctata ctattaaagc atgtaggtct
    20401 tgataatata ttcttttgga gaggtataca tcttggtaaa caggttcaat caccatattt
    20461 taagtggaaa agcttaaatg gagaaagtta tatatatgca gtaaatatgc cacatggata
    20521 tggaactggt atgcttttag agccttcatt aaagtatgtt aatggacgct tagacccagc
    20581 tattgatttt attaaatcat atactgatgt agatgaggtg ttaatacctt ctgggaatga
    20641 ccagttaaat ataataggtg atttcaaaaa taaaattaaa gcgattaatg atatcggaaa
    20701 gtattcatat ataacgagtt catatcaaga gtttttaaaa tatataaagt ccatagaatc
    20761 actagaaagc tatagaggag aatttagaga acctgtatta gcaagaatac ataaatcaat
    20821 tggttctagt agaatggata taaaattagc atgtgataaa ctagaaaaca agctgattaa
    20881 aagaatagag ccattattag taattgctaa aaagagtaaa attgaaataa gcaatcagtt
    20941 acttataaat acatggaaaa aacttttgga aggacaagca cacgatagtt tagctggatg
    21001 tgtaactgat actgtaacag atgatattct tcaccgtata agagaagcaa atgaaatatg
    21061 tgacagtatt gaaaatacta ttgtaaaaaa gatttcagaa gggcttaaat tatctaaaaa
    21121 tgatatttta gtatttaata cagaagctaa gcgttttaat ggatataaag agatacaagt
    21181 tgtaagtgat agtaaaaata tttattttaa agataattta gatgctacta taattgaaga
    21241 aacttatgta aaaccgagag aaaatgtatt agaagaaaca cctgctggta atatatttat
    21301 tgaagaacca ggatattata tattaaaagt aagaataaat gtacagcttc ctgcacttgg
    21361 ttataaagtt gttagttttg aatcaagtga taaagaaatg gtgtcactta ataaatctaa
    21421 tgatgctttt atatctaatg ataattataa gataatatat gaagatggaa gcttaaattt
    21481 acaacttaaa gatggaactt acatatcaaa ctttttaaca ttaaaagata gtggtaatgc
    21541 aggtgatacc tatgactttt ctccactaaa aaatgatgag gatataaaac tttcgtttga
    21601 taaagtgtat acagaaaaag ctttaggata tgaaaaaatg gtggttaaag gtactacttt
    21661 actaccactt acgttagaag atagaaaaaa caaatatctt aatgggaaat taaatgtaaa
    21721 agtaagtatt gtcttatcca aagaaaatcc attgatggat gtaaaacttt gtgttgataa
    21781 tacgatatat aatcatagac tacgtgttca tataaaaact gatataaaag ataataaaaa
    21841 catagcatca ttgccatttg gatatataac aagagaaaat ggagttttag acaactggga
    21901 agatatttac agtgaaatgc ctatagattt agaacctttg gaaagtaata ttactctaac
    21961 aaaccaaaat agaagctgta ctatatttac taaaggaata aaagagtatc aacatattga
    22021 agatgaaatt gcattgacct tacttgctac tacagatgaa ctaggaaagc ctgatttatt
    22081 atatagacca ggacgtgctt ctggtgatac aactaaaaaa ggtcacataa gaattaaaac
    22141 agataaagct caaatgctta agaaattaga attttcattt gcaatatata tggattctaa
    22201 atcatttgat gaattggaaa ttgcgaattt aacccacaat tatctaaggg aatgtgtaaa
    22261 ttatcaatta caggattaca atttcttttt atatcgtata gataataaga ttcaaaaatc
    22321 aattgtcaat aaaactgtaa gtagagaatt agaaataata tctttaccag aaaactatct
    22381 tatatcagca tgttatcctt cttattacag taaagataaa tttataataa gagtggaaaa
    22441 tccaagtagt aataaaatga tattggatga gcaaatattt aaaaatagaa atgggaaaat
    22501 tgtaaatgca atagaagatg tagaagagga acaggtatat gaaatagcac cttttgacgt
    22561 tatttcaata ttattggatt tataagtgta aaagtaagtt tattgtttaa agtctgacag
    22621 atattaatct gccagacttt ttaatttgta taaatttggg taaatattaa tgaatggttt
    22681 tagtatttaa gtatgaattt agtatttaat atagaattta ttgaagtaga ataattagtt
    22741 taacaggata agcagtttaa ataaagaatt attatattag agtgatttta tatataattt
    22801 aagataataa aattttaata aaatgagtta aagattgtat attaatagag ttgatgtagt
    22861 tacaaaaaag ttaccaattg ttatgtattt acaaaactga tatattaatt acataaaatc
    22921 taaaatagtt tgtaatagga gtatatat
//
LOCUS       DAMJYX010000053        22905 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJYX010000053 DAMJYX010000000
VERSION     DAMJYX010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.053"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..321
                     /locus_tag="PC479_002183"
     CDS             <1..321
                     /locus_tag="PC479_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2804724.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="PC479_002184"
     CDS             337..1920
                     /locus_tag="PC479_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2804725.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="PC479_002185"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="PC479_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2804726.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="PC479_002186"
     CDS             3475..4827
                     /locus_tag="PC479_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2804727.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="PC479_002187"
     CDS             5019..6128
                     /locus_tag="PC479_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2804728.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="PC479_002188"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="PC479_002188"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2804729.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="PC479_002189"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="PC479_002189"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2804730.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="PC479_002190"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="PC479_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2804731.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12637..13659
                     /locus_tag="PC479_002191"
     CDS             12637..13659
                     /locus_tag="PC479_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2804732.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13719..14726
                     /gene="gap"
                     /locus_tag="PC479_002192"
     CDS             13719..14726
                     /gene="gap"
                     /locus_tag="PC479_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2804733.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14948..16150
                     /locus_tag="PC479_002193"
     CDS             14948..16150
                     /locus_tag="PC479_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2804734.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16176..16919
                     /locus_tag="PC479_002194"
     CDS             16176..16919
                     /locus_tag="PC479_002194"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2804735.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16928..18460
                     /locus_tag="PC479_002195"
     CDS             16928..18460
                     /locus_tag="PC479_002195"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2804736.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
     gene            19048..20340
                     /gene="eno"
                     /locus_tag="PC479_002196"
     CDS             19048..20340
                     /gene="eno"
                     /locus_tag="PC479_002196"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2804737.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            20933..21157
                     /gene="secG"
                     /locus_tag="PC479_002197"
     CDS             20933..21157
                     /gene="secG"
                     /locus_tag="PC479_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2804738.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(21541..22479)
                     /locus_tag="PC479_002198"
     CDS             complement(21541..22479)
                     /locus_tag="PC479_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2804739.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(22467..22865)
                     /locus_tag="PC479_002199"
     CDS             complement(22467..22865)
                     /locus_tag="PC479_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2804740.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVK
                     INLNIKGYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
BASE COUNT         9105 a         2624 c         4430 g         6746 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta gaaataaaat tatttatagt aataaatatt agaatgaaaa
    12421 tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg atttgaaaat
    12481 ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac tattgaaatc
    12541 tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca ctagggacaa
    12601 aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt agaaattcaa
    12661 aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat tttaagacaa
    12721 atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga gattagtgaa
    12781 agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga cgtagctgta
    12841 tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa ggacatcatg
    12901 agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct tggaataaaa
    12961 agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa agatgtaact
    13021 agatatggat gcgaatattt cttaggcatt ttaaaagatg gagatatcgt ttcaataaca
    13081 ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag atatccaaac
    13141 tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca atctaacaat
    13201 atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca tataccagat
    13261 gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa gacaactttg
    13321 gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga tgtcatggca
    13381 aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa tgcagttgga
    13441 gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact gaacactgtt
    13501 ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt tgcagggact
    13561 gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt tcttgtaaca
    13621 gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc tagcacagag
    13681 ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta gctataaatg
    13741 gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa gataagttcg
    13801 aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta tttaaatatg
    13861 atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct tttgtagtaa
    13921 acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca tgggctgaat
    13981 taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat aaagctgaag
    14041 ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact ggagatttaa
    14101 aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca gttatatcag
    14161 gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat gataaatacg
    14221 gtatagaaaa aggcttaatg actactatac atgcatacac taatgaccaa aatactttag
    14281 atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga aacatagttc
    14341 caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta aaaggaaaat
    14401 tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa ttagtttgta
    14461 cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa gcttcaaacg
    14521 aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga ataagctacg
    14581 gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa caattagtta
    14641 aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata agaactttag
    14701 gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt agattgacaa
    14761 agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa aaatatataa
    14821 aatacaccta aataatgtta catactatat ataaaaatat gataatatga tatatttaat
    14881 agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat taaaaggaga
    14941 gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt gcggaaaaaa
    15001 agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta ctgatgaaaa
    15061 tagattgaac ggagctttac caactattca atatttaatt tctaaagggg caaaagttat
    15121 attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat ctttagcacc
    15181 agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag cagacgataa
    15241 tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag atgtagtatt
    15301 attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt tctctaaaga
    15361 actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc atagagcaca
    15421 ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat acttaattca
    15481 aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat ttacagctat
    15541 acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat tagaaaaagt
    15601 tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc agggttatga
    15661 agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa tgatggaaaa
    15721 agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg cagaccactt
    15781 tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag attacatggg
    15841 actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag aatcaaaaac
    15901 tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa atggtacatt
    15961 atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg gcggtggaga
    16021 tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg tatcaacagg
    16081 tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg cagcattaga
    16141 taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata attgcaggaa
    16201 actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa gttaaagata
    16261 aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta ttaaaggatt
    16321 taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg cactttgaag
    16381 aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata gatatggact
    16441 atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat gaaactgtaa
    16501 acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt gttggagaaa
    16561 ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa gttgaaaaag
    16621 ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat gagccaatct
    16681 gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt atatcttata
    16741 taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga atacaatacg
    16801 gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat atagatggag
    16861 ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt aatttctaag
    16921 gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg gatacaataa
    16981 ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata gaataaaaaa
    17041 agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac ctgatggtca
    17101 aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag tatatcaaga
    17161 tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata aagttttatg
    17221 tgaagcaatg gataatgcaa aagaaaactc tcttcatgta atgggacttt tgtctgatgg
    17281 aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa aagataaagg
    17341 cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc ctcaaagtgc
    17401 attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag gtgaatttgc
    17461 tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa gagtagaact
    17521 tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg aggaagctat
    17581 tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag ttattatgaa
    17641 agatgataaa ccagttggaa gtataaagga aaatgattca attatattct tcaactttag
    17701 accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg atggatttaa
    17761 aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa ctatagagaa
    17821 tgtgcatata gcatttggac ctcaatcttt agccaacact ctaggtgagt atctagctaa
    17881 aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg taactttctt
    17941 cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga taccttctcc
    18001 aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga cagataaagc
    18061 tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg caaatcctga
    18121 tatggtaggt cacacaggaa gtatagaagc ggctataaag gctgttgaaa ctgtagatac
    18181 ttgtgtagga agattgatag ataaaatagt agagttaggt ggaagtgcaa taataactgc
    18241 tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg taacggctca
    18301 ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg caaaattatt
    18361 ggatggagga agattatctg atatagcacc tactatttta gatatgatga agttagaaaa
    18421 gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga taagtagata
    18481 ataatatata aactacaata taaatttaaa aataatgcag tttaggttta ttttgtaaag
    18541 tttagacttt atgacataag gtttaaattt tgtaagttct ggatattgta aggtgtagat
    18601 gaagatatat tttataaaat ttacaagata gacaatttat aaaataaaaa attacaatat
    18661 agataataat gtaaataatg atataaataa taacaagata gacaataaca atataaataa
    18721 taataattaa tatatattat ataagcaaaa attttagaat cattagttgt acattttatg
    18781 tttaatgttt taaatctagt tataatattt gtgtatttaa gattggtttt ggattttaga
    18841 caagtttttt tatattatat aaattaaaat attataaaaa ataaaataaa aataggaact
    18901 tttacaaatt tacccatcaa ataaatttgt acctaatttt caaaagcaac tttatgtact
    18961 ataatataga aagtagatta aaatagtaga cggatgttta cagtaaatga aaatttaact
    19021 aatagaaaat cgaagggaga tatacaaatg tcagttattg aattagtata tgctagagaa
    19081 gtattagact caagaggaaa tccaactgta gaagttgaag tagtattaga agatggagca
    19141 atgggaagag caatagttcc atcaggagca tctacaggag cttttgaagc tgttgaatta
    19201 agagatggag ataagggaag atacttagga aaaggtgtag aaacagctgt tgctaacgta
    19261 aatgaaataa tagcacctga aatagaagga atggatgcat ttgaccaacc agctatagat
    19321 gcaatcatga tagaattaga tggaactcca aacaaaggta aattaggagc taatgctata
    19381 ttaggtgttt caatggcagt agctagagca gctgctgatg aaataggttt accattattc
    19441 caatacctag gtggagtaaa tgctaaacaa ttacctgttc caatgatgaa catattaaac
    19501 ggtggagagc atgctgataa caacgttgat gttcaagaat ttatgatatt accagtagga
    19561 gcttgttgtt tcaaagaagg tttaagaatg ggagcagaag tattccattc gttaaagaaa
    19621 gttttaggag aaaaaggatt agcttgtggt gtaggtgacg aaggtggatt tgctccaaac
    19681 ttaggttcaa acagagaagc tttagaatta atagttgaag ctataacaaa agctggatat
    19741 aagccaggtg aagacgttat gttaggtctt gacgttgctg ctacagaaat gtacaacaaa
    19801 gaaactaaaa aatacgtttt agcaggagaa ggaaaagagt taactgctgc tgaaatggtt
    19861 gctttatatg aagattggtc aaacaacttc ccaataataa ctatagaaga tggtttagac
    19921 gaagaagatt gggatggatg gaaattatta actgaaaaat taggaaataa attacaatta
    19981 gttggtgacg acttatttgt tactaacaca gagagattag aaaaaggaat tgaaaatgga
    20041 gtagctaact ctatattagt taaagttaat caaataggta caataactga aactttagat
    20101 gctatagaaa tggctaagag agcaggatat actgctgtta tatctcacag atcaggagaa
    20161 actgaagatt ctactatagc agatttagct gttgcagtaa atgctggtca aataaaaact
    20221 ggtgctccat caagaactga tagagttgct aagtacaacc aattattaag aatagaagaa
    20281 atggttggag agcaagctag atattgtgga ttaaaatctt tctataactt aaaaaaatag
    20341 ttattaaaat ggttactaaa aaagattaat gaagagagaa tataagatat ttaagctcat
    20401 cttataagat ataaaagaga taagtgttaa ttgatttttc aattcactta tcctttttta
    20461 tattttatta cttaatttta ttcatagatt cagaaaaaat atgaataaat gtttctatct
    20521 catttttatc taattgcata ggaggttttt attaaaaaat aaaagtgaag acatattttg
    20581 atttaatcat aattgtcatt gaagtattat tgctaaatgt ggattattta ttttaaatta
    20641 tgttgaaatt aaaagttata tcaatatttt aaaatgattg ttttattttg gtggctattt
    20701 ataatatata caatgagagt agattatttt aagtgttaat ttatgtatta atttaaatag
    20761 ttttctcttc taagttttat tcatttttat attgagaata tagtaatata ttatagtaac
    20821 aatatatttt tttgtatgtt atttatagca taatagtctt gaaatggcta tattattgtg
    20881 atacactaaa gagtaatgaa atttaagtaa taaacagata ggaggggcaa gaatgagcaa
    20941 cattttaatg ggtgtgcaag tagtactagg gattttacta gtaattagca taatgcctca
    21001 agattctaag aatgctttac cttcagaatt tggtggagaa ggaagtcaag cttactttaa
    21061 accaaaagga aaacaagcat ttcttgctag agttacaaag ataacagcag tattattttt
    21121 tataaatgca ttggcattac ttatagttaa gaagtagtga tattgttgaa taactggcca
    21181 ttatttaatt agtgcatttg ctagacattt aaaaattaaa tttagttttt aataaaagta
    21241 catcaacata aaaagtacgt aaaataaaag ctggactaat gattttatat gattagtcca
    21301 gtttttattt tatagattga ctatttggtg gcgtttatta tttgtattta tatattgttt
    21361 gtatttatat attttcttca aactaaaaaa tatatagcaa cactactttc accatgttta
    21421 atgagataat atacacaaag ttatattata tagtaaaaat agtataataa tatgttttta
    21481 cgcgattaaa tcagttttat atatgtttct tataatacca atttgtgtta tcaaatatca
    21541 ttaaataaga ttattctgct ttaaaataaa tattattaaa aatactgtaa ttatagaaaa
    21601 tccagaagta aatactacaa tttgtccagc taattcactg tcagaatcca tctgttgtgc
    21661 cattgtaaat gaagaaactg cagttggagc agataacatt atcataagtg aagctaattc
    21721 tacatttcta aatccaagaa aaatgctaat tggtaaaaat atagcaggta caagtataag
    21781 ttttccacat acgcccaata taatgggttt tatatgcccc tttatagcag aaaaactaaa
    21841 agagccgcct agaattataa gtgataaagg agtagctatt ttagaaatat caccaatagt
    21901 tttgtgtaaa aatgtaggta tattaatatt taaaattagc attgtcaatc ctataaaaga
    21961 ggcgattatg agtggatttg ttaagacgct tttaatcata tgttttatat ttactttgcc
    22021 acctctaaaa atttcaagaa ttacaacaga taatacatta aacataggta caataactgc
    22081 aattaataaa gatgtgactc caatactctt atcgccaaat agtgaaattg tcacaggaat
    22141 accaaatata acgaaattgc tacgaaatac accttgtatt aatgcacccc ttgtcttatt
    22201 atctttttca attttaggaa taataaagac taagattaag aagattgaaa aaataattat
    22261 aacagctaca gccataagct taaaattaag agaaccatct aaatctgtat tgtatatatt
    22321 aaaaaataat aataatggca agaaagactt gaagcaaaca ttattcattt tatttaaagt
    22381 atgagtatca aatagaccta atttttttag aacatatcca agtgacattg tgagaaacaa
    22441 aggaagaaca acatttattg ataggattaa attttccaag gtttactctc cgtgattcta
    22501 tgatttgata attcaattaa atttccatta gtttttttca aataaataga tttcatatta
    22561 ccaaagacac ctgtacattc tacaattcct ctcttaattt gaatctcatt ttgttctaag
    22621 tatttttttg gctcatcaat atttgtgttt attttaaaac ataagttatc tctacttgct
    22681 tttgaatttt taacataagg taaaaattta taacctttta tgttaagatt tatcttaaca
    22741 tttctagcat taagtttata atttttgctc atttctttag atgaaaaacc aaatttttta
    22801 taaaaattga tataattttc tatattatta ttagtaatga ccacatgttc tatttttttt
    22861 atcatggcaa ctccttaaga ttttatataa attagttaaa tttat
//
LOCUS       DAMJYX010000054        22840 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJYX010000054 DAMJYX010000000
VERSION     DAMJYX010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.054"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            33..1166
                     /locus_tag="PC479_002200"
     CDS             33..1166
                     /locus_tag="PC479_002200"
                     /inference="COORDINATES: protein motif:HMM:NF025330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HDF2804741.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASTLGSPFVSTD
                     SFSTTKTKIQTFKSTLATNLTKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGVTSALQQYPPCVAVENYIYFLCNNSLGLSLLRYDTLTNVNSVLAKPSVCDDAL
                     MVYYDGYIYRIGGYSNSSANSTCQKYNIKTNTWSYISNMITPRGGTCGNTYSNEIHVL
                     YGYTDTNSNSTASAISEYYKISTNTWSTKTSFPPSRDRRLFVCAKTGTYYFCITGATD
                     PNNIWETYINRYDAGNGALTKIEGWYKSFATSINDMLYTVYYGDTYLYVKGYNQNGRV
                     FSKEYVKKNSTFPYNPLLKAVSVKNKYIYYYENNMYKCFIPEL"
     gene            1184..1549
                     /locus_tag="PC479_002201"
     CDS             1184..1549
                     /locus_tag="PC479_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804742.1"
                     /translation="MIKIWDKKEKINGVSAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKNLTALETAEKYLEMHKKQEEDIKAHEELEKNSKNATIATHEEVK
                     ALREETATLTFAMMEKELI"
     gene            1550..1684
                     /locus_tag="PC479_002202"
     CDS             1550..1684
                     /locus_tag="PC479_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2804743.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIVGEDYIV
                     "
     gene            1895..2500
                     /locus_tag="PC479_002203"
     CDS             1895..2500
                     /locus_tag="PC479_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804744.1"
                     /translation="MIEKLNENASLSDLITTFENVKDELQIGKNNIASTLGNPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            2524..2829
                     /locus_tag="PC479_002204"
     CDS             2524..2829
                     /locus_tag="PC479_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804745.1"
                     /translation="MTEYVEVGRRIFFDEEGKIIFYEGQSKGNVPDRKNIKKIEYIDL
                     EYDYVDYDKYKIIGIDIRTKQPILEEIPVYMSEEGKRIQELENQILIAENEKSGGLL"
     gene            2829..3011
                     /locus_tag="PC479_002205"
     CDS             2829..3011
                     /locus_tag="PC479_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804746.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            3045..3611
                     /locus_tag="PC479_002206"
     CDS             3045..3611
                     /locus_tag="PC479_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804747.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            3642..3932
                     /locus_tag="PC479_002207"
     CDS             3642..3932
                     /locus_tag="PC479_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804748.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            3936..4193
                     /locus_tag="PC479_002208"
     CDS             3936..4193
                     /locus_tag="PC479_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2804749.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            4454..5317
                     /locus_tag="PC479_002209"
     CDS             4454..5317
                     /locus_tag="PC479_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF2804750.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            5396..6208
                     /locus_tag="PC479_002210"
     CDS             5396..6208
                     /locus_tag="PC479_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2804751.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            6365..8656
                     /locus_tag="PC479_002211"
     CDS             6365..8656
                     /locus_tag="PC479_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2804752.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFY
                     SKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVVLVGNGLDNSQKELIKNKKF
                     KNITQIGGNGNEKAFNEVENLVK"
     gene            complement(8698..8883)
                     /locus_tag="PC479_002212"
     CDS             complement(8698..8883)
                     /locus_tag="PC479_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF2804753.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(9401..9577)
                     /locus_tag="PC479_002213"
     CDS             complement(9401..9577)
                     /locus_tag="PC479_002213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804754.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            10294..10971
                     /locus_tag="PC479_002214"
     CDS             10294..10971
                     /locus_tag="PC479_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2804755.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            11140..13176
                     /locus_tag="PC479_002215"
     CDS             11140..13176
                     /locus_tag="PC479_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2804756.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(13367..13933)
                     /locus_tag="PC479_002216"
     CDS             complement(13367..13933)
                     /locus_tag="PC479_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804757.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            14221..15156
                     /locus_tag="PC479_002217"
     CDS             14221..15156
                     /locus_tag="PC479_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2804758.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            15621..16835
                     /locus_tag="PC479_002218"
     CDS             15621..16835
                     /locus_tag="PC479_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804759.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(17255..17776)
                     /locus_tag="PC479_002219"
     CDS             complement(17255..17776)
                     /locus_tag="PC479_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2804760.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            18132..18929
                     /locus_tag="PC479_002220"
     CDS             18132..18929
                     /locus_tag="PC479_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2804761.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            19039..19629
                     /locus_tag="PC479_002221"
     CDS             19039..19629
                     /locus_tag="PC479_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2804762.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            19695..21392
                     /locus_tag="PC479_002222"
     CDS             19695..21392
                     /locus_tag="PC479_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2804763.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            21606..22445
                     /locus_tag="PC479_002223"
     CDS             21606..22445
                     /locus_tag="PC479_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2804764.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         9195 a         2412 c         3713 g         7520 t
ORIGIN      
        1 aaaggatact taaaaaagga ggcaggaatt taatgactat aaaattagaa gaagattcaa
       61 gtataaaaga agttatggac tcttttgaga atataaaaaa tgaccttcaa aatgctaaag
      121 acaacttagc tagtacatta ggaagtcctt ttgtttcaac tgattcattt agtactacta
      181 aaacaaaaat acaaactttc aagagtacat tagcaactaa tttaactaaa aaaggtgttt
      241 caacttcttc aaatgaaacc gtccaaaata tggtaagtaa agttgatgat attgaactag
      301 gaaatacaga cataccaaac tggtataaac ctaaaaatat aatttttgat ggagttactt
      361 cggcattaca acaatatcct ccatgtgtag cagtagaaaa ctatatatat tttctttgca
      421 ataacagttt aggtttatcg cttttacgct atgatacatt gacaaatgtt aatagtgtct
      481 tagcgaaacc atcggtttgt gatgacgctt taatggttta ttatgatggt tatatttaca
      541 ggataggtgg atatagcaat agttcggcaa attcaacttg ccaaaaatat aatataaaaa
      601 caaatacttg gtcgtatatt tctaatatga ttacacctag aggtggaaca tgcgggaata
      661 cttattctaa tgaaatacat gttttgtatg gttacacaga tacaaatagt aattcaacag
      721 caagtgctat cagtgaatat tataaaatta gtacaaatac ctggtcaact aaaacaagct
      781 ttccgccatc tcgagataga agattatttg tatgtgcaaa aacaggcacc tattattttt
      841 gtataacagg agcaacagac ccaaataata tttgggaaac ttatataaat agatatgatg
      901 caggaaatgg agctcttaca aaaatagaag gttggtataa aagttttgca acaagtatta
      961 atgacatgtt atatactgtg tattatggcg atacatatct ttatgtaaaa ggatacaatc
     1021 aaaatggaag agtattttct aaagaatatg ttaaaaaaaa ttctacattt ccatacaatc
     1081 cactacttaa agcagtttca gtgaagaata aatatatcta ttactatgag aataatatgt
     1141 ataaatgctt tatacctgaa ttataaaaag gagaaaataa aaaatgataa agatatggga
     1201 taaaaaagaa aaaataaatg gagtttcagc agaagaaatt ttaaaaggta attatgattt
     1261 tcaaacaagt gaagtatttt taatacttga tgattataac agagttacaa atatagagtc
     1321 agttaataca attaaatcta tatataaact agataagaat ttgactgctt tagaaacagc
     1381 agaaaaatat ttagaaatgc ataagaaaca agaagaagat ataaaagcac atgaggaact
     1441 agaaaaaaat tctaaaaatg caactatagc tacacacgaa gaagtaaaag cattaagaga
     1501 agaaacagca acattaactt ttgcaatgat ggaaaaggag ttgatataga tgtggtataa
     1561 aataataaaa aaatactatg aggatgaatt atggactaaa gagcaagtta aaacagcagt
     1621 agaaaagaat aagataacaa caatagaata taaaaatatc gttggagagg attacattgt
     1681 atagtccttt tttaatttta tatagtaact atttgtcaag aaagagttac taaaaagctt
     1741 tatacttgtt tattttgctt gtttttctat taaaaataaa attatttata ctatatatac
     1801 tttgaaaaaa ttacttaaaa tcaatcactt ttctatttta aaaagtaacc atttcttgac
     1861 aaacagttac taaaaataga aaggtgatat ttttatgata gaaaaattaa acgaaaatgc
     1921 tagtctaagt gacttaataa caacttttga gaacgtgaaa gatgaattgc aaattggtaa
     1981 aaataatata gctagtacat taggaaatcc ttttgttgga actgataagc taagtgtaac
     2041 taaaaccaaa atacagacct tgaaaaatac actagcaatt agtttgtcta tgaaaaatat
     2101 atctgctcaa tcaactgaaa cacttcattc actaattggt aaagtagctg gaatcgcaaa
     2161 tatttttatt gcacaaggag aatgtactca agttggagaa aaaacttttc aactctatca
     2221 agtagataat aatacgccaa aaacttgttc agcaaacgca ataagtttta acttagcttt
     2281 tactccaaaa tttattattg tctatagaaa tgcttacaat ataggtttgt gtagttttgt
     2341 tttttactca tcttttcgta atattataac agcagtgcct caacctagca attttggtga
     2401 aatgtacatg cctaacacat tcgattgtgt agtaggtcac acttgtattt tgccagtgaa
     2461 agattacaaa ataccacatt ttttctatgc aattgcttaa aaaataagtt aaaggaggaa
     2521 actatgactg aatatgtaga ggtaggaaga agaatatttt ttgatgaaga aggcaaaata
     2581 atattctatg aaggacaatc gaaaggaaat gttccagata gaaaaaatat aaaaaaaata
     2641 gaatatatag atttggaata cgattatgtt gattatgata agtacaaaat aataggaata
     2701 gatataagaa ctaagcaacc aatcttagaa gaaataccag tatatatgag tgaagaagga
     2761 aagagaatac aagaattaga aaatcaaata ttgattgcag aaaatgaaaa atcaggagga
     2821 ctattataat gaatataaat aatgttgtag taagaatatt agcagagaga atattaaacg
     2881 gagggttaaa cccattgaaa aatagagaat ttcagttaga tgacgtgact aacacagagt
     2941 acagaaaggc tatagaggat tatattataa aaaatagtgg agtagtagaa ggagcagaac
     3001 caactatata gagggttctt tttttattga aagaaggtga ctaaatgact tttaaagagt
     3061 tagttaataa agttagaaat cttgtattag aagcaaagaa agtaaccata gaagatacag
     3121 aaaataagtt tacaagtgaa aatgtagaag aagcattgaa agaatgtata aatagagcag
     3181 atgaggcttt tcaagaagcc gatagtggaa aagagttaat tgcaactgct atcggctctc
     3241 ctgctacatc agaacaaaca tttcaagatt atgcgaacta tattacagga tttaagagca
     3301 atataagcaa tttagaaact caattgaaaa gcaaatattc tattagatat ggtcctattg
     3361 atggatatga tggtaatcct ttttctgcta attttggcaa gagtgcaagt taccttattg
     3421 tctatgttta ctttagaaga agtgtatatt attataatcc cagtggtagt tctttaggaa
     3481 gtaatacagg aggttctgaa cgtgcatgga ttactataga cagcaataaa actggttttt
     3541 cagttcattc atatgatact agttatgagc catatccttt tacaggttat tatattgctt
     3601 gtttcgcata ataaattata ttattaaaac taggaggatg tatggaagaa attagcataa
     3661 atctattatg tgcagttgca ggagttgtaa tatcctactt agcatttaga aatagctcaa
     3721 acagaaagat acaagatgat acagaaacaa ctacaaaatt agaacaacaa ataacttttc
     3781 tgtgtgagaa tgtaagagat ataaagcatg atgtagcaaa gtttaataca agtttcttag
     3841 atatcagtga acgagttgca aaagtagaag caagtacaaa acaagcacat cttagaattg
     3901 atgaaattat aaatagaatt ggaggaaaat aaaagatgga caatttaata agttttatac
     3961 cagagcagtt gctaatttta gtagctgctc tttatgtttt aggtgttgga ttcaagaaat
     4021 ataaacaatt agataataaa tacattccag tagtgttact gatacttgga ataggtttct
     4081 caatatggat gctaggatta aatcctgttg cagtcttaca aggtgtaatt tgttggggag
     4141 ttgcaatagg tataaatcaa acttacaaac agttgaagga tggtgaaaag taatgaaatt
     4201 aacaaaaata ttattactca caaaaattta aaaaatttca ttaaatagag tatactttta
     4261 tagctaataa gtatataata atagtacaga actcgtaaag catttatatg ctcaaatata
     4321 cgggacattg atttttgaag gaacccgcca agcttctttt atagctcaaa taggcgggac
     4381 agataaattt acccactaat attaattggt gggttttatt atataaaagt atatctatga
     4441 aaaggtgaaa acaatggttg aagtaaaaga agaaaaaaca tttgatgaac aaatagatat
     4501 tttaaaaagt agaggattaa taataaatga taaagaagat gctaaatttg tattaagtaa
     4561 tgtcaattat tataggttta cagcatatct tctaagtttt aagaatgatg atggctcata
     4621 taaagaagga actacgtttg aagaagttta tgatatatat aggtttaata aggaatttag
     4681 gatattatta acagatttgt tagggagcat agaaatagca tttaggacat acattgcata
     4741 tacattagca attaaacatg gtgcttgtgg atatctagaa agggagagtt tcaaagatga
     4801 aaaattctat attaattttt tgacagcatt agagagagaa aaaagtaata attcagataa
     4861 gctttttatt atacaccata aagaaaaata tgaaggaaaa cttcccattt gggttgcaac
     4921 ggaaataatg acttttggta tgttgtcaaa attatattca aacatgttgc cagaagatac
     4981 tagatacata aaaaataatt tgtgtagagt gaatacttta ttagttaaat cttggttaca
     5041 atcattaaca caggtcagaa atcaatgcgc tcattatggt agaatatata ataataattt
     5101 ccgtattata acaataaaaa acgaatataa aaagtataac ttggataata aaaagatatt
     5161 ttcttatata cttgctatga agcatttgac tatggataaa ttaatttgga atagtttttt
     5221 tataaaactt caaaagttaa ttaatgatta taataattct atagacttaa agcttattgg
     5281 ttttcctaat aattggatag agatattggc taaataaaat agttacttta agaagtttat
     5341 aaacacttac tatatgtaag tgttttttta ttgaaaagaa ggaggaaaat aaataatgaa
     5401 aatatgtatt acagtaggac acagtatttt aaaaagtggt gcatgcactt ctgctgatgg
     5461 agtagttaac gaataccaat acaataaatc tcttgcacca gtattagcag atacatttag
     5521 aaaagagggt cataaggcag atgtaataat atgccctgaa aagcagttta aaactaaagc
     5581 agaagaaaag acttataaaa tacctagagt taatagtgga ggatatgatt tacttataga
     5641 actacatcta aatgcaagtg atggtcaagg aaaaggttca gaagttctat attatagtaa
     5701 taaaggttta gaatatgcaa ctagaatatg taataagcta ggtacagtat ttagaaatag
     5761 aagagctaaa ttagataaag gattatatat cttaaatagt tcaaatccta cagcagtatt
     5821 aattgaaagt ttcttctgtg ataataaaga agattatgag aaagctaaga aactaggtca
     5881 tgaaggtatt gctaagttaa ttgtagaagg tgtattaaat aaaaatataa ataatgaggg
     5941 agttaaacag atgtacaaac atacaattgt ttatgatgga gaagttgaca aaatctctgc
     6001 aactgtagtt ggttggggtt ataatgatgg gaaaatactg atatgtgata taaaagatta
     6061 cgtgccaggt cagacgcaaa atctttatgt cattggtggt gcagcatgtg agaagattgg
     6121 ttctatgact aaagaaaaat ttactatgat aaagggtaat gatagatttg atacacttta
     6181 taaggcatta gattttatta atagataaat taaaaagtag caactaaaag tagttgttat
     6241 cttctaatgc ctcattcata ataaaaaaat aatttattat ataataatat taaagtttgt
     6301 ttctaaggtt aatattatgt gatacaattc aagtgggtaa atattatttg aatgaggtgt
     6361 ggggatgaaa aatttaagca aaaaagcagt tattttatca ctttcattgg tagttgtatc
     6421 acctttggta cataagataa atgcacaaga tattgagaaa aatagggaag ttagatatga
     6481 aacaaggaaa gctaataact tagaaaatat gacaatggaa gaagcttttc cagatgaaaa
     6541 ttttagaaaa gtaatttgtg ataaactaag tattttagat gatggaaatc caattggaat
     6601 ttcacaaaaa gcgattatag aatctacaaa aggtttaacc ttagggagca aatcaataaa
     6661 aaatttatct ggaattaatt attttattgg attagaaaat tttagttgta gaggaaataa
     6721 tttaacgagt ctagatttaa gtagcaacat aaatttaaaa gaattgtatt gtaacgaaaa
     6781 tcaattaaca agtctagatt taagtaataa tagagagtta acaacgttgc attgtggaga
     6841 aaataatcta acagacttag ttatagaaaa ttctaaatta gacgaattga actgtagaaa
     6901 aaataattta aagagtttag atataactaa agcaactaat ttgacaactc ttttatgttt
     6961 tgaaaatgaa ttgactagtt tgaacttatc taagaatcag aaattaaaaa ttttgaaatg
     7021 tgattataat aatttaaatg atttagatat atctacaaat ttagaattac aggatttaaa
     7081 ctgttctttt aatcaattag aaaatataaa tttagataat aataaagaac ttgttaagtt
     7141 aatttgtcac aacaataaat tgcaaaattt aaatttaaaa aatcatgaaa agcttgtaaa
     7201 actatattgt aatcaagaac aattaagtac tttaaatgtg gaaaattgta ttaatttgga
     7261 agatttaact tgtagtagaa gtaatctgaa gaatttggat ataagtagca ataagaattt
     7321 aaaatatcta gagtgtgtag caaacaagtt aacaaatttg aatatagaga ataatataga
     7381 acttttagaa ttatactgtt atgacaatga aataaaagct ttagatataa gtaaaaatat
     7441 tgacttagaa aatttaactt gttataaaaa taaattagat agtttaaata caagtaaaaa
     7501 taaaaatctt aaatacttat attgttcaca aaatgaatta actagcttgg atgtaacaaa
     7561 aaatacagaa cttgtaaggc tatattgtgg ggaaaataga ttaaccagct tagatgtaag
     7621 caaaaacaca aaattaaaag aattagattg gtcaaatcaa aaaaaatcaa catcaacagg
     7681 aggttcgtca gggagtggag gaggttcatc atctacaaat gaagagtcct ctgaacctac
     7741 ttcaacacca tctaaggaaa aattaacagg tgtagacaga aatgaaacat cagtgaaaat
     7801 aagtcaaaaa ggatggaata aagctgataa tatagtgtta ataaatgact ctagcatatc
     7861 ggatgcttta tcagcaacac catttgctaa gtctaaagat gcaccaatat tacttactaa
     7921 aaataataac ttaaataaat taacagaaaa agaaataaat agattagaag caaaaaatgt
     7981 gtacatagtg ggcggattaa agtctgttga tgaaaaagta gtgtctgatt taaagaaaaa
     8041 aggattaaat gtaattagaa tatctggaaa tgatagatat gaaacatcaa taaaacttgc
     8101 aaaagaatta gacaaaaatt ctaacctgtc aaaagtagta gtggtaaatg gagaaaaggg
     8161 actagctgat gcagtaagta tgggagctat atcagcaaaa gaggaaatgc caattcttct
     8221 tactaatcaa aatgatgata tgaaagatat aaaagactta atagctaata aaaacatatc
     8281 aaaatcatat gtaataggtg gagaatcttt atttaataat aaagaagtga ataatacatt
     8341 accatctgta actaaaatag caggttctga tagaacagaa actaactcaa aagtaataag
     8401 tcatttctat agcaaagata cacttaatga cttatatgta gctaagaatg gaatgaataa
     8461 acaagacgat ttagtagatg ccttatcagt aggagtgctt gcaggtaaaa ctgaatctcc
     8521 agtagtatta gttggaaatg gattggataa tagccaaaaa gagttaatca agaataaaaa
     8581 gtttaaaaat ataactcaaa ttggtggaaa tggaaatgaa aaagcattta atgaagtaga
     8641 aaacttagtt aaatagattg aaaggtagta actggaaata gttactacct ccttttttta
     8701 ttcattttca gttttttcaa taattatttt tccatcctca aaagtaacaa gaacatccct
     8761 ttcatcagga gagatattca tctctttaat ccatgacatt ggaagtatca atctgggtgt
     8821 aaaagagcct ttgccacttt tattaaatcc aattctaagt tttctttgct ccaataatat
     8881 cattcctttc tactattaat aaatgccagc aataataaag taaataccaa agcaataaga
     8941 gctttaaaag taaaacttat tattatatta ataatattta aaactatagt gataccaaga
     9001 ataatcatac aaattctata aaatttatta ttcatattaa attagaaaat gtgttataat
     9061 attagaaaga agctacctag aaggaaggta ttttcctcct aggattgaaa cttataattc
     9121 tttaatcaat cttagtattg cgagtactag attaattaaa actaatgtga aagagaaagc
     9181 atttttaatt ttattaaatt tgttttctct tttatttttt cttctttcta atctccccac
     9241 tttcttacct cctttctata tttatattat atcatcacgt gacgtgaaag ccaataaata
     9301 ttccatattt ttcaataaag ttataaaata tattaatata aaatacaaaa tataataaaa
     9361 ataagtggat gtttacatta tacacccact tattttagag ttattcctca tcagattttt
     9421 ttattataat ctcatcttcc ttaaaaataa ctacagcctt acgtttttcc cttgtaaatc
     9481 ccattttttc aagttggctt aaaggtaaag aaattttagc cgaataagta cctgaaccac
     9541 ctttactgta aactatttta gattcttttt gctccaaact attttcctcc ttttttatat
     9601 tgtaatgtaa caaatattaa caataaaatg cttactatta atcctattat attgttgata
     9661 ttaaaatgta aaaataaaag tattatgtct attactatag ttatagcaac tataataaga
     9721 cagaatttat aaactttatc actcatattt tatagaaaat gtgttataat atttagaaag
     9781 aagtcactag gaagaagatt aaattcttcc tagcttacaa aactataaat cttttaataa
     9841 tttaattagt ttatcaatta aatttattaa aatgggtaag atagagagag cagttttaac
     9901 tttggcgagt ttgctttctc tatctttttt tcttctttct attctactca ttttcctttt
     9961 cacccccttt ctatatttat attataacat gccgaaccgg cgaaaacaat atttatttcc
    10021 atatttttca ataaagttat attgataaac tatatttttt acaaataaaa ataaatggtg
    10081 acaaaaccaa acttgccacc cttttgccac cgaaaataaa taatagaata aatttaaatt
    10141 aaacataata aaacataaac aacaaaactg accttaataa aacattgaaa taccaacact
    10201 tgtaaaaaat aacaacaata aacaaataac ctagaataat attacaactt aagtaaataa
    10261 taattttgaa taataattta cggaggtatt catatgttta aacacgataa agcattatta
    10321 aaagaagtaa aagtagaaag accaaatcct caatatgctg ttttaatgca agaacagttg
    10381 ggtggtgcaa atggagagtt aaaagcagct atgcaatact tgtcccaaag ttttagaata
    10441 aaagaccctc aaataaaaga tttatttctt gatatagcag cagaagagct tagccatatg
    10501 gaaatggtag cacaaactat aaatttatta aatggacatg atgttgatta taatgcagtt
    10561 aatacaggcg aaatagaaac acatgtatta actggtctat caccagtctt aataaactca
    10621 tcaggagctc cttggactgc taattacgtt acagttacag gggatttagt agctgactta
    10681 ctttcaaata tagcttctga acaaagagct aaggtagttt atgagtactt gtatagacaa
    10741 atagatgata aatatgtaaa agaaacaata gatttcttac ttaatagaga agaagcacat
    10801 aatgctctat ttagagatgc tttaaataaa gttaaagata caggttcaaa tagagatttt
    10861 ggagttacag aagattctaa attatatttc gatttgtcta ctccagggcc aaatcacgat
    10921 actaagatag atattaatcc tccttctttt gaaaaacctt taaaaaaata atttgatatt
    10981 ttttattttg ttttagtagt tataaaaatt atttactaga agaattaatt atatgaaaaa
    11041 ttaaatatgt atagttaaaa atgggtatac acttattata agtgtatatc tatttgttta
    11101 ttgcaaaaca tataataaaa taacaaatga ggtggtattt tggacagtaa tagaaaacct
    11161 aataatctaa taaatgaaaa atctccatat cttttacagc atgcttataa tcctataaac
    11221 tggtatagtt ggaatgatga ggcttttaaa aaagcaaaag aagaggataa accaatattt
    11281 ttaagtgtag gatattcaac atgtcattgg tgccatgtta tggaaaaaga atcttttgaa
    11341 gatgaagaag ttgctgaaat aatgaataga aactttgtgg caataaaagt agataaagag
    11401 gaaagacctg atgtagatag tgtgtatatg acagtttgcc aagctatgac aggtagtggt
    11461 ggatggccaa tgaccataat aatgacacca gataaaaaac ctttttttgc gggaacatat
    11521 ttcccaaaat attcaaggta taatagacct ggagttatag atttattaga gaatgtatct
    11581 gaaaagtgga atacaagcag agatatatta ataaaatctg gagatgaaat aatagaagct
    11641 cttaaggatg attttggtgt aaaaaatact gagggtgatt tatcaaaaga gatgttgagt
    11701 tcatcagtta gagtatttaa ggctatttat gatgaaaagt atggtggttt tggtaatgct
    11761 cctaaatttc caagtcctca gaatcttatg tttttaatga aatattatag tatagaaaaa
    11821 gacaaagatg tgttaaaaat ggtggaaaaa actttagatg gaatgtatag aggaggcttg
    11881 tttgatcata ttggatttgg gttttcaaga tactctactg ataaaaagtg gttggctcct
    11941 cattttgaaa aaatgctata tgataatgct atgcttacta tagctttttt agatgcgtat
    12001 aaaataacaa agaaagagtt gtataaagaa attgctataa aaactataga ttatgtagtt
    12061 agagagatga aggataaaga aggtgggttt tattcagcac aggatgctga tagtgaaggt
    12121 gaagaaggta aattttatac atttaatcct cttgagataa ttgaagtttt gggtgaagaa
    12181 gatggaattt tctttaataa ttattttgat ataactagta gtggaaattt tgaaggcaaa
    12241 agtattccta atcttataaa aaataaagaa tatgagagac ataatgaaaa gatagctgac
    12301 cttagtaaaa aggtatttga atatagaaaa gaaagaacat ctttgcataa agatgacaag
    12361 atacttacat catggaatgc attgatgata gttgctttaa caaaggcata tagtacttta
    12421 aaaaatgaca tatatttaga gtattcaaat aagtgtctta actttataaa taataatctt
    12481 gtaaatgagt caggtagatt attagctaga tatagagatg gaagttccga ctatttagca
    12541 tatttagatg attatgcttt tttaatctgg gcgtatatag agctttatga gtcaacattt
    12601 aatatgaaat atttagaaaa ggctttaaat ttgaatgaaa gctgtatcaa ccttttttgg
    12661 gattatgaaa aaagtggatt ttatatatat ggtaaagata gtgaaaattt aatagcaagg
    12721 ccaaaagatt tgtatgatgg agctattcca tcaggaaatt ctgttcaatt atacaatctt
    12781 ataagacttg cgaaaattac tggagataat agacttgaag aaatgtcata taaacaatta
    12841 aaactgtatg tggataatgt aaagagttct ccagctggct acagctttta tatgctttca
    12901 ttgatgtttg aactttattc tactaaggaa attatatgta tttttaagga agattctgat
    12961 ttaattgcct ttaaagaatt gataagtgaa aattttatac caaatgcaac ttttttagca
    13021 aagaaataca atgaagaaaa cactattata ggttttttaa ataactatag attgaaggat
    13081 gataagatta gttattacgt gtgtcaaagt aatagttgta gtcaaccaat taatgattta
    13141 caaaaattaa aagatatgat tttaggagta gaataatcat aatagtgaaa aaggagatgc
    13201 tttaaaataa aagtctaatc atctcctttt tttgtacaaa aaacagaaaa agagttcctg
    13261 agaacaccta atctatgtaa tgattgtttt gttaaaaaat aaaaatgaca ttatagttat
    13321 ataaaatatt gtactataaa aaagtagtat tatagatgcg atagtattaa tcgtttttat
    13381 ttactctctt tcttcttttt gcaaattctt caagtgcttc agatttaatt ttatctattt
    13441 cttcatattc cataaaatta gcatcttctt caaagccatc ttgttcttca ccattaaaac
    13501 gatacatttt ttttagtaaa ctccataagg tctctaattc atctttggta aattcgtgaa
    13561 atacatctgc cataaagttt attccagtcc tactacatgt taccataacc ttttttccta
    13621 agtctgtaac tttaacatta attgctctct tatcatgagg acttggaatt acatcaacat
    13681 atccattttt ttcaagatta gcaactagtc tatttatatt ttgtttactt gtacccattt
    13741 ttcttgcaat attatttaat gttgtttctt cctctggtaa atgaagaatt gataaaatag
    13801 tcatatactg tcttgaagta agtataccaa aatatttatc tccttctatt tgtattttgt
    13861 ttgtaagggt aaaaagagtt gcatatgttt gattcattaa aaagagttct tttagttcat
    13921 ttgaataatc catgaaatat atctccttta tttttagatg ttgttttgtc gaaaacagaa
    13981 tatcatgaat taattctaat gtcaatatgg tgtcaaaata tattaaagta ttactttttt
    14041 atgtaactat taattaatta aattattgta taattttatg taaaaacttc tatgtaccaa
    14101 tgataacttc tatgaggaat taataattct taaatttttt agaaagattt tgacttgaaa
    14161 atacttgtaa tataatatat aaattaatat actgaataaa atcaatctgg agggataatt
    14221 atgaagtgta caaactttac tttcaaagga gaggaaggtt tagatatata tacatataaa
    14281 tgggaagatg aaaatataaa aaaaccaaag gcagttatcc aaattgcaca tggaatggct
    14341 gaaactgcac aaaggtatga gacttttgcc aaggtactta ctaaaaatgg ttatatagta
    14401 tatatcaatg accatagagg tcatgggaaa actgcaaaga taatagaaaa tgttggacat
    14461 ttagctgaaa aagaaggatt tagatgtctt gtagaggaca tgtatacttt aacaaatatt
    14521 ataaagaaag aaaatgaaga tctgccaatt tatctatttg gacacagcat gggttcattt
    14581 gcaagtcaaa gatatataat ggattacagt aataatttat ctggtcttat actatgtggt
    14641 tcaaatggaa agcaaggaat cattctaaat cttgctcatc tgataatcaa tcgtgagatt
    14701 aaaaagtatg gaagacgttc taaaagtaat aaaataaata atttgatatt tggcggcgaa
    14761 ataataagaa gaaatgaaaa gactaagttt gactggttaa gtagagataa agaacaagtt
    14821 gaaaaatata taaatgaccc attttgtgga gtagtgtgta gttgtggctt tttttatgat
    14881 ttagttcaag ggttaaaaga aattgaggat aaagaaaact taaaaaaagt gccacttgat
    14941 atacctattt acataatatc tggagataaa gaccccatag gaaaaaatgg taaaggagtc
    15001 ttaagactaa gggatagata tataaaatta ggagtaaaag atgtaacttg caaactttac
    15061 aaagatggaa ggcatgaatt gttgaatgaa ataaatagag aagaagtttt tgaggatata
    15121 atttgttggt taaataataa aatagaaata ttgtaataaa aaaatataat gatatattta
    15181 ctttaaattt atttatatat aatatttgaa aagtggatta tgtatatgca aagtagaaaa
    15241 gagagagccc agaagtgatt ttttgctata atgccattta ttgaataggt gggtgttatt
    15301 ataaataaat tattaacact cagtattatt atcattgtat atggaattta ttttatttaa
    15361 aattccttga aagtttaaat atagtaagtt gtagtaaaga gaaaatataa atatttaaat
    15421 gctatttaat gtgaatctat taattaggtt atacttaaac ttaattaata gattttatat
    15481 ttcaagacaa tgagataggg ttttagatta aaatataacc aatggttaaa ttacagacat
    15541 tttgttaact ttccctcgat ttactagtaa atattttttt tatatactta aattacaaca
    15601 gatgtactag ggaggtatat atgcaaatta aaataggtaa agtcatacag tgtcttagaa
    15661 aagaacgaaa tttaactcaa gaacaattag caaagtttat aggtgtgtca acaccagcag
    15721 tatctaagtg ggagagtgga aattcttacc cagatataga attattgcct ttattagcag
    15781 acttttttaa tgtgtctatt gataagcttt taaattacaa gattgattta agtgaagaag
    15841 aagtaatgaa aatttataaa gaattagaat caggctttgc tagaattgaa attgatttat
    15901 caacagaaga accaaaagaa gaatttagac aagatttaga atctgtaaaa aagctttcca
    15961 atatgtatat agagaagtat cctaaaagct atttattgaa gctaaggata tgttcgctgt
    16021 atcagatgta ttcatataaa tttggtaaga gtgaacttaa tgacagagta aaagaaacaa
    16081 cgaatattct tgaagatata gtgagaaata cagatgatat ccaaataaaa gaaacagctc
    16141 ttattatatt atcaaacgca tattgtatgt tggaggatta tgaaaaggca gagttgtatt
    16201 taaatatgat acataagtca ataggggata caagtgttaa tttagctatg atatatttaa
    16261 agcaaaatag acttgaagaa gcagaaatat tgcttcaaaa taaattgttt agtaatgtat
    16321 ttaatataag catggattgt aagggaataa ttaatgtata caaaaatcaa tacaaggaat
    16381 taaagaaaaa attggagaat agaaatttta ataaaaatgc aattgagaga gaaatggagt
    16441 atataaaaaa taagttgctg gggtatgcaa atctttcttt agaaataaaa aaaatgctca
    16501 gtgaagataa aggtgcattt tttagtatgt atatggatta tatggaatta tctctaattt
    16561 ttttattttt taacatgaaa gaagaagcaa aaaaagcttt atatagtcta aaagaaatat
    16621 tagaaaaata tcctatacat gaaagtttgg atgtaagtca aatgagattt tttgataagg
    16681 tagagtctaa aaatttatat acttttaata tatatacaaa cttgttaatt gtacttaatg
    16741 atgacagtta taatgagctt agagaagaac caatttttaa ggatgtaatt gaaaagattt
    16801 tagatatgga aaaaatgttg aaaaataaag aataagtaaa aatttaatat aggacttaaa
    16861 gattaatcaa attaaaagaa agtgtttcaa aataaaaatt tgaatcacct tttttgtcgg
    16921 tcttaaacag ttatttagaa aaatagaggt ttcaaaatag aaatctgaat tatctgtatt
    16981 attacacaaa aatagaaacc taaatttata aaaatagaat aaaatttgtt atataaaatg
    17041 taattttgtc tataaaatct cacctgtttt cttaacttta agcgtgaaaa agaggatgta
    17101 tcatgaattt tttagttcat gatgcatcct gctttgtatg aaatccaata tatctttatt
    17161 atttaaacaa tggaaaagag tcttatttat attttgagac atctctttta tttaattatg
    17221 aagttttata cagttcaata aataggaatt atatttagaa actcatgaca ttatttctta
    17281 gatagtgatt aaatttatca tattccattt tagataactt ttctgggtaa gacattacaa
    17341 tcaggttatg cccattaaat tttttatggt atggtggatg aatgtgttca taattatttg
    17401 tcttatattt tactctttca tagaacgcct tgcgataaat tgaaatctca tcaattggaa
    17461 ggtcaatttc taaaataata gtttttcctt tcttatttaa aagctctaac gctctttgcc
    17521 catatctatt attgcgaatt tctggaaaaa tgcagaaatg ttctacatag ataaagttat
    17581 ctgtttccca acataaaatt atgccgacaa actggttttt atcataaatt aaattgaatt
    17641 gatattcttt atttttcaaa atttctgctt gcaaagaaga ttttctttgt ttatgaaatg
    17701 ggaaactaat tttatataat tccatagcct tgtaatataa tttattttca ctggttctta
    17761 accgttcaaa tttcacttta ctcctcctaa tcacgaaaat tccaatttat ttatgagttc
    17821 attttaccat agtcagtatt tgcatacaat gtgttttaag ttgagtgtca tttctttctt
    17881 ttatttatgc ttatatctca tagtctaaga gataattgct taagacataa ctaaaaacta
    17941 ttatattaag ctttgataat ctaaaaacag tatatttatg gacttttcta ggtctaaaag
    18001 taatcttaat aattttagaa gttacaaaaa agttacataa atttggaaag ctataatata
    18061 catatataat ataaggttaa aactgtaatg aataatatta atttaaaatt tgaacatgga
    18121 aggagattat tatggagaaa agagaaaaga ggataataat atcatcttta ttaagtgtat
    18181 caattttaat gggtttggca agtatatatt ctatattaaa taaagaagac ataagtttaa
    18241 ccgttaaagg tcaagagcaa aaagtatctt cctttaagaa aacagttgag gaacttttgg
    18301 atgaacaggg tgtaaagtat aattctgaag ataagattaa tccaagttta gatacagagt
    18361 taaaagatga tatgaaaata aaagttgtta aggtaactaa aagtaaaaaa gaagagattg
    18421 aaaaaattcc atttgataca aagcatgtaa atgatagtaa tttgttaaaa ggaaaatcta
    18481 aagtttatca agaaggtcaa gaaggagaaa aaaaactagt ctataattta acttaccatg
    18541 atggcaagtt agtcaaaaaa gtcttatcaa aagaagtaat atctaaggag ccaactacaa
    18601 aaattataaa atatggaact aaagaaaaag tactaatagc atcaagagga gcaaatataa
    18661 gaggaggcaa acatatgaaa gtggttgcca ctgcatatgc aggagataca ataacatcta
    18721 ctggtacaac tccaagatgg ggtgttattg cagttgaccc acgtgtaata ccatatggaa
    18781 caaaagtata tatacctaaa ctgggtatga cttttgtagc agaagattgt ggaggtgcaa
    18841 ttaaaggtaa tagaatagac atttttatga acagtgaagg aaaagcttca aattggggaa
    18901 ggaaaagtat agatatatat ctacattaaa ttagtgaata gaataacttt acttgaagga
    18961 caaaaaagta taaaatatga attaactaaa ctgttagatt attcttaaaa cataaaaata
    19021 tatctaaggg gggataagat gaaaatagtc tgcttaggag acagtttaac gtatggattt
    19081 ggagtatcac gaagtaattc atggacaaac attgtaaata aagagactcg attagaaata
    19141 gtaaataagg gtataaatgg tgatacaaca agtggtatgt tagctaggtt taatgaagat
    19201 gttgtaaaaa actctcctga tatagttttt ataatgggtg gcaccaatga ttttattgct
    19261 ggtgcgggaa atgaaattat taattctaat ataatggcta tggtgcatca gtcatattca
    19321 aaaaatatta tacctataat aggtatacca ttaaaaccag atattccaaa tgttagggaa
    19381 gattggagtt gttttactga ttttaatata gtatctcaaa acttagaatc atatagttat
    19441 tggataaaaa aattttgtat gacatttaat actaatttcg tagattttta ttcagaatat
    19501 aataaaaata tggaactaga aggatataaa aaattatatt ttgatgggtt acatccaacc
    19561 aaagagggtc atagaataat ggcagacatt tttattaatt caattaataa gtatatagaa
    19621 gagttataga ttacattaaa atgcttgttt atgcgtcagt tgcaataaat acatacagta
    19681 gagaggggtt tattatggat tatactaaaa cttatgaaga gtggataaag ggttcatatt
    19741 ttgatgaaga tacaaagcta gagttggaga atataaaaaa taatgaaaaa gaaatagagg
    19801 atagattcta taaggattta gaatttggga ctgcgggtct tagaggaata attgaagcag
    19861 gaacgaatag aataaataag tacacagtta gaagagctac ttttggattg gcaaactata
    19921 tattagaaaa cacaacaaaa gaagaaacaa gtagaggagt agtaatagct catgataata
    19981 gacacaagtc tagacaattc tgtatagaat ctgcaaatac tttggcagca tgtggtataa
    20041 aagcatatat atttgatagt ttgagaacta ctccagaatt atcttttgct gttagaagct
    20101 taaatgcaat cgctggtata gtcataacag caagccataa tcctccagaa tataatggat
    20161 ataaggtata ctgggaagat ggagcacaag ttatgccaga gattgcaaat gcaataacag
    20221 agaaagtaaa tagtatacat gattatagta caataccaac cttaacggaa gctaataaaa
    20281 atttggtagt tttgttggac gaatcacaag atactaaatt tatagaagca gtaaaaagcc
    20341 aaattataag aaaagattta gttaaaaatg taggtaaaag ctttaagata gtttatacac
    20401 ctctttgtgg aacagggaat gttccaataa gaagagcatt gaaagaagtt ggttttgaaa
    20461 atataattgt agttccagaa gaagaaaatc cagacccaaa ttttgcaggg cttgattatc
    20521 caaatccaga agagaaaaaa gctttaaata gaggtatatt actcgcaaaa gaaaaaggag
    20581 cagaccttgt aatagcaact gacccagatt gtgatagagt aggtgtagca gtaaaaacta
    20641 ctacaggtga gtatgcttta cttacaggaa atcaaattgg tggtatgctg acacattata
    20701 taatagaagg attaaaagaa aataataaat taaaagaaaa tcctacaata ataaaaacca
    20761 tagttacatc agaatttgga gcagatatag ctaaagcaaa taatgttgat gtattaaatg
    20821 ttcttacagg ttttaaattt attggtgaaa aaataaaatt atttgaacaa aataaaaata
    20881 gaagttatgt atttggatat gaagaaagtt atggttactt agttgggact catgctagag
    20941 ataaagatgg tgtagtttct tcattgttaa tatctgaaat ggcagcattt tactattcaa
    21001 aaggtatgag tttatatgat ggcttaatag aactttataa aaagtatgga tttttcaaag
    21061 aacagactat atctttgact ttaaaaggta tagaaggtgt agaaaagata aaagaaataa
    21121 tatcttattt tagagaaaat caaattgatt atataaatag tattaaagta gttgataaaa
    21181 aagattataa aaatggtata gataatcttc ctaaatctga tgttttaaaa tactttttgg
    21241 aagatgaatc ttgggtagct ataagaccat ctggaacaga accaaaactt aaattttaca
    21301 tagctgtaaa aggagctagt gatattgaag cagataaaaa gattcaaggt ttgaaaaagt
    21361 atatagatga catggtcgaa aaattaaaat aaaatattat tattttataa gataattttg
    21421 taataatatt tatttagctg aaaattataa tttactttca ttgagtagaa tatggaaatg
    21481 ttagtagaat tgttattttc agcttttatt tgtgttattt ttacttaaat aattatatta
    21541 tagaatgaat ctgagtataa ttaatattgt ataatgaaat taatttgata gaaaagggga
    21601 tatttatgtt tggtatagat aagaaattga ttgaatatat aattgcaaaa tccaattaca
    21661 gtgaattaag ttcaaagtca gcagaagtat ctttcttctt aatgttgtcc atatttcctt
    21721 ttttaatatt tacaataagt tctatagctt atataccgat tcttcattta aataagtata
    21781 tagctttgtt tagaaatatg atgccagaag gtgcttttgc tgtgttatct tcaatcatag
    21841 tttctgcaat agacaataga aacctgaaat ttttagcagt cagttttgta cttactatgt
    21901 ggacattttc aagagcagta aaagctctta ttaaaggaat gaatagagcc tataaagtta
    21961 aagaaactag gtcttttttt aaaattttat ctatatcatt tttatttaca ataatgcttt
    22021 tggtccttat ttttttatct atgatatttt tagtttatgg ggaaaaaata ggttatttta
    22081 tttttaatct tgtaggattg gatgagatat ttataaaaat atgggatatt ctaagataca
    22141 ctgtaggtat aataacaata atagtgatat ttacactttt atataaatac actccaaata
    22201 aaaagttaac tattaaagaa tctgcgccag gtgctatttt tgctactttt gcttggtttt
    22261 tagtatcatt tttatattct tattatacaa attactatgc taactatgaa gtaatctatg
    22321 gaagtatagc tgaaataatt gtacttatga cctggatgta ttttagtagt tggtctatcg
    22381 ttattggcta tgaggtaaat tcaagattgt attttagaaa gataagacat gaaatgttaa
    22441 agtaaagtat ttaaaataaa agagagtatt tagtattata aatgtaattg aattaataaa
    22501 gaaatattga catttaagtt aaaaagtcta gaatttttct gggctttttt tatttttagt
    22561 ataacttgac aaattttatt taatgggata gttttaattt ataatcatct ttaaaaagat
    22621 gactattgat aagtaagaga taaaattggt aagtatcaaa aaatatacta aataaaaatt
    22681 atagtcctaa tgagttaatt tctgtatttt caaataatgc agtatttagt catgtgtttt
    22741 tatatgcgcc tgtatttcta attaccaata tattagaata agctactgta attagattta
    22801 gaaaaaagcg acataaataa ttataaacaa ataataaatt
//
LOCUS       DAMJYX010000055        22838 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJYX010000055 DAMJYX010000000
VERSION     DAMJYX010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.055"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(431..1096)
                     /locus_tag="PC479_002224"
     CDS             complement(431..1096)
                     /locus_tag="PC479_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804765.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            1440..2870
                     /locus_tag="PC479_002225"
     CDS             1440..2870
                     /locus_tag="PC479_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2804766.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            3238..3816
                     /locus_tag="PC479_002226"
     CDS             3238..3816
                     /locus_tag="PC479_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2804767.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            3846..3992
                     /locus_tag="PC479_002227"
     CDS             3846..3992
                     /locus_tag="PC479_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2804768.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            4288..5667
                     /gene="scfB"
                     /locus_tag="PC479_002228"
     CDS             4288..5667
                     /gene="scfB"
                     /locus_tag="PC479_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2804769.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            5696..6193
                     /locus_tag="PC479_002229"
     CDS             5696..6193
                     /locus_tag="PC479_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2804770.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            6491..8155
                     /locus_tag="PC479_002230"
     CDS             6491..8155
                     /locus_tag="PC479_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2804771.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            8168..10618
                     /gene="recJ"
                     /locus_tag="PC479_002231"
     CDS             8168..10618
                     /gene="recJ"
                     /locus_tag="PC479_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2804772.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            10631..11143
                     /locus_tag="PC479_002232"
     CDS             10631..11143
                     /locus_tag="PC479_002232"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2804773.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            11217..13424
                     /locus_tag="PC479_002233"
     CDS             11217..13424
                     /locus_tag="PC479_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2804774.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            13449..13898
                     /locus_tag="PC479_002234"
     CDS             13449..13898
                     /locus_tag="PC479_002234"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2804775.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            13913..14527
                     /locus_tag="PC479_002235"
     CDS             13913..14527
                     /locus_tag="PC479_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2804776.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            14618..16174
                     /locus_tag="PC479_002236"
     CDS             14618..16174
                     /locus_tag="PC479_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2804777.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            16164..17426
                     /locus_tag="PC479_002237"
     CDS             16164..17426
                     /locus_tag="PC479_002237"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2804778.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            17500..19287
                     /gene="aspS"
                     /locus_tag="PC479_002238"
     CDS             17500..19287
                     /gene="aspS"
                     /locus_tag="PC479_002238"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2804779.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            19746..20978
                     /locus_tag="PC479_002239"
     CDS             19746..20978
                     /locus_tag="PC479_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2804780.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            20992..21798
                     /locus_tag="PC479_002240"
     CDS             20992..21798
                     /locus_tag="PC479_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2804781.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(21910..22779)
                     /locus_tag="PC479_002241"
     CDS             complement(21910..22779)
                     /locus_tag="PC479_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2804782.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         9084 a         2367 c         4116 g         7271 t
ORIGIN      
        1 tttataagta aaaataattg attactagtt aataaaataa atgtgatatc caaataaatc
       61 ctaaatctaa tttgagtttt aatatagata gtctaaagtt tgattattaa agatttaatt
      121 gtaagttgtt acagttgatg atattgatta gagattaaag tttagagtag aaagttacct
      181 taaatttaaa aatgcaagat aacccaatct aaaaattata atcactcaat aggtttaagg
      241 atttatttgt ttattataaa taggagggtg tctcaaaatg aactttttag tttatgaggc
      301 actctctttt atatagaatt aaatatattt tattatttca acaatgaaaa agaagcttat
      361 ctctattttg agacagcctc tttgtatact attattaatt taatcaataa cataatttaa
      421 aactatatta ttagttaaga tttcctgctt tattagtaaa taattcttta atagcattgt
      481 ttctggaaac taaatcgtta tcagagttgt ttgttttttt cataatcact gaataacgtt
      541 ttttatttat aaaaaatatt aaaatcttat catcatcaaa attgtattct ttatatctgt
      601 caatagactc ataacctaga tttagaatgt ttttgtcttt tatactatct acatctaaaa
      661 tatatgatgg gtcagccttt agtaatatat taattaaaca tttttcttca ccagcacaga
      721 ataactccat cttacttgat atagatttag attgctctga agttattttt gcaggaataa
      781 ggttgtcatt gtcatctctt agaagataac catgtgtgtc ttgttgatat actaaattaa
      841 cctttttctt aggaacacta atcattttat ctaaatgtac ataatgatga ttttcaacaa
      901 aaccttcatt taatatttca acaaagttat taattttacc taggcaaaaa gattctatag
      961 attctttaca gaatttattt gtaaatggta ttatgtttac gttttcctcg tctgtattcc
     1021 atgctatagc ataatccaac gaatatactt cttcctctat atctgcaaaa ttatattgta
     1081 ctatgtttcc tttcaaaatt actcgacctc catcttataa tatatttaat atactttata
     1141 aaacttatat tatatttaaa taatatcata aattgaataa atatgatata gtaaataaga
     1201 aaaaatttga aaaaaatcac tataaatgag taataaatat aaatagtgta tactaatggg
     1261 atggaaaatg tccactatta actttattac cccacatata taagataaaa ttattaatat
     1321 aaaatataat ctatcgacaa tgaatagact tttaaataaa cttaatataa aattaattaa
     1381 ataagtgctt aaaaacgatt tactttaatg atagaatcat attaaaggag gataatttta
     1441 tggatataaa gaataaaatt aaatctatat ttttaccaga aaaaaatgac tatttagatg
     1501 atgaagaatt tgagtatgat atagaaaata atgaagaaga tttaaaggaa gataacaaag
     1561 tacatttaaa tgatttaagc agagtaaaat atgattacag agagttaaaa gacatagaaa
     1621 accaaacaga agagatgtgt ctgctagcag taaagcagga tggtactata attgagtttg
     1681 taaaggacaa gacttataaa gtatgtatgg aagcagtaaa acagacatat aaatctctta
     1741 agtatataac aaatcaaaat gaggatattt gtatagaagc agtaaaacag aactacagag
     1801 ctttgtacta cataaataat aagacagaaa atgtactaat tgaggctata aaaaatgcat
     1861 caacttatga tgtcatggaa gtatttaaat ttgtggaaga gcaaacagaa gatgtatgtc
     1921 tagcatttat agaaagggca tctaaaaatg atgttgcaga gatattaaaa ggaataaaag
     1981 agcaaactcc agccatatgt ttagaagctg taaaaaaaga tggaaaatct ttagcttatg
     2041 taaaggaaca aagtaatagt atatgtttag aagctgtaaa agaaaactac agtgcactat
     2101 cttgtgtaaa agagcaaact gaagaaatat gtatagaagc agtaaaacag aatgactttg
     2161 cattgtatta tgtaaatgaa cagacagaaa aaatatgcat ggaagcagta aaaagaagtt
     2221 atatggctct acagtatgta aataagcaaa cagaagagat ttgtttggaa gcagtaagaa
     2281 ttgatggaag agcattacag tacgtaaaag agcaaacaga agaaatttgc ttggagtcag
     2341 ttagacaaaa tggaaaagta ctgcaatatg taaaaaaaca gactgaaaat atatgtatag
     2401 aagcagtaag aggttcattt gaagaacttg aaataaaaga aatattaagt tatgtaaaga
     2461 taccaacaga aagaatattt gtagaagcag taaagcaaaa tggaaaaatt cttaaatatg
     2521 tagaaaatca gacagaatta atatgtttag aggcagtaag agaaaactat aatgctttag
     2581 cttatgtaaa ggaacaaaca gaaaagattt gtttagaagc agttaatcaa agttatgaag
     2641 cgcttaaata tgtgaaagaa caaacagaag aagtttgtct aaaagcagta aaacaagatt
     2701 atagaatgtt gaaatatgta aataatcaaa cagaaaaaat atgtttagaa gcagtaaagc
     2761 aaaattatag agctttagaa tttgttgata atcaaacaga aaaagtttgt cttgaagcag
     2821 taaaacagaa tagaaaagca ctacagtatg taaaacaaaa acaaagttaa aaggctaaaa
     2881 tatatgggga atatatgcaa ataaataaaa tttgatattt tgtaataaat aaaatgggta
     2941 tctcaaaagg aactttttag tttgtgaggt actcttttta tatgaaatga aatatatttt
     3001 tagcctttta acaaagggga aaaggtttgt ttctattttg acataggctt tttatgcata
     3061 taaagcttga taaaattgtt ttattataat tgttttgtta tataaataat aagaagaaaa
     3121 ttatataggt ctttttttga ccagttttac atttttaaca actagttttg taaagtatat
     3181 tgaattttct caaaaaagaa tgtactctat atatgtaaaa tttttttgga ggtaaaaatg
     3241 tttaaaagat atgctgaaaa aatgacatct gttttaattt gtaataatat gatagataat
     3301 aatgaatcta aagtctattc ttatggattt gaaatattaa tagctttcat agttaatata
     3361 accaccatgc tgtttatagg ttttttattt ggtaaattta catatgtatt atttttttta
     3421 atgtgttatt gtccaataag acaattttca ggagggtatc atgcagacaa ttattttaga
     3481 tgcttattaa cttttatttt tataatttta tccacaatct taattataga aaatataaat
     3541 atagacttat ttaaaaatat tattatgatt attgcaagtg ttagttgggt tggaatttgt
     3601 gttctgtgcc caatagaaca taggagtaac cctataagtg atagggaaaa gttggtttat
     3661 aagaaaactg ctatatttat atctacagta gtgttattga taacaatttc aagtctttct
     3721 ttgagtatat ttgtagatta tttcacatat agcgcatttg ccatgttttg gatttttgtg
     3781 atgttagtat taggtaagtt aaaagctaaa gtataagtaa aatatgtatg agggagagtt
     3841 aagttatgaa aaagtttatt gtaagattta tgaagtttgc tagctcattg gcacttagca
     3901 ctgctatttt gtcagctaat agtacctgtc cttggattat tcatcagcct aaagtaccca
     3961 aagaaatcag caatctaaag aagacaaatt aaatacaagc acttatttat acaaaattat
     4021 caggaaaaaa gaaattatta tattttaata agattaaaac aatataaaat cagatacaaa
     4081 atttatttta taggagccta atacctattt ggtaatatat tagtgcgcca atttaggggg
     4141 atatagtgta tttaatgaaa tgacttacgg agagtaagtc atttttgtat tgtatgtatt
     4201 ttatgtaaac ttatgatata atgaagggta aagtttaatt aaaataataa aaaactttag
     4261 tatagatagt aatttaggag gtagggtatg agtatgatac ataaattttc tatgaatgga
     4321 tataatatcg ttttagatgt gaatggtgga gctgttcatg tgttagatga tgttgcatac
     4381 gatttgttag atttttataa agaaaaaagt aaagaagaaa tattagagat attaaaatca
     4441 aaatatcaag aagaaaaaat aaatgaagca tatgaagaaa tattaaattt ggaaaaagag
     4501 ggtcttttat atacagaaga tacatatcaa tatcatccaa gctttgtaca tagagagcct
     4561 gtagtaaaag ctttatgttt aaatgtagct catgattgta acttaaaatg taaatactgt
     4621 tttgcagctc aaggtgattt tggaggagaa aaagaactta tgagttttga agttggtaag
     4681 gcagcaattg actatttgat tgctaattca ggaagtagaa agaacctaga gatagatttc
     4741 tttggtggag agcctttgat gaactttgaa gttgtaaaac aactggttga ctatggaaga
     4801 agtgtagaaa aagattataa taaaaatata agatttacaa taactacaaa tggtgtactg
     4861 ctaaatgatg agattataga ttatataaat gaaaatatgc ataatgtagt tttaagttta
     4921 gatggaagaa aagaagttaa tgataatatg agaccaactt taaatgataa aggaagttat
     4981 gatattacat taccacgatt taagaaactt gtggaaaaaa gagcaaaaga taagtattat
     5041 tatataagag gtacttttac aagagataat ttagattttt caaaagatgt tatgcatttt
     5101 gcagatttag gatttaaatt aacctctgta gagccagtcg ttggagatga gagtaatcca
     5161 tatgcactta gagaagaaga tttacctaag atatttgaag agtacgaaaa atttgctgta
     5221 gagtatgcag atagacaatt acaaggagat ggatttaaat ttttccattt tatgatagac
     5281 ctaaatcaag gtccatgtgt aataaagaga ataactggat gtggagctgg aaatgagtat
     5341 ctttctgtaa ctccaaatgg agatatctat ccatgtcatc aatttgttgg aaatgaagaa
     5401 tttaaaatgg caaatatatt tgatgaagaa atagtacttc cagaaaattt aaaaaatatg
     5461 tttagagaag ctcatgtata tactaaagaa gagtgtaaac aatgttggaa taagttttac
     5521 tgttcaggtg gatgtcatgc aaatgcgata aactttaata atgacatatc aaaaccatat
     5581 gaattaggat gtgaaatgca aagaaaacgt acagagtgct ctataatgat acaagctaag
     5641 ttaatgttag aaggagcaac caactaaatt tatattttaa aggagttgtt tatatatgat
     5701 tagagattat ttagaagata aacctttaat tgacgaaagt gtatttgtgg caaaatcagc
     5761 agatgtaatt ggaaatgtta aaataggcaa agattcaagt atatggtaca atgcagtagt
     5821 taggggtgat gaaggaccaa taactattgg agaaaacact aatatacaag attgttctat
     5881 agtgcatgga gatacagaaa ctataattgg taacaatgta acagttgggc ataggtctat
     5941 agttcatggt tgtaaaatta gtgataatgt gttaattggt atgggttcaa taatattgga
     6001 taatgctgaa ataggagaat atactctgat aggagcaggt acactaatta catctaataa
     6061 gaagtttccg ccaggtgttt tgataatggg ttctcctggt aaggttgtta gagagcttac
     6121 agaagaagat aaaaaatata ttgatgaatc ttatgaatgg tatttagagg cagcacaaaa
     6181 tcaaaaatat tagtttaaag agtggttgtt tcaaaacata tttaaatctg ttttgaggca
     6241 accactaatt tattttatta gattttgttg atattttagt tttcaaaaag tatttgatta
     6301 aaccagatat aaaatgtcaa aaataaaaat taactgagtt aaataatttt aataaaattg
     6361 gtagtatatg tgataaaatt aaatattaaa gaggatatag gatattagaa tatcaattgg
     6421 tgaaataatg tcatatacga taaattaagg tgtaagtttt tgggtatacc tatcttagga
     6481 gtgaaaagat ttgagaataa gttatagaaa tttaaagcga tatagagaaa ttggatatgt
     6541 tttaataaag tatggtttta gttttattgt agaaagacta aatatagaag gaattgccta
     6601 taaaatacca ctatttgacc caccagaaga aataaaaaat atgacaactg gtgagagaat
     6661 gaaaagagtt ttagaagaac taggacctac atatataaaa ataggacaga tattgagtac
     6721 aagaaaagac ttgttagacc aggatataat tgatgaaata tcaaaactca gagatgatgt
     6781 agagaaattt gattcaaata tagctattga tatatttaaa gaagaagtgg gtttaagtat
     6841 agaagaaata ttcttagaat ttaaagaaga agcaatagca gcagcttcaa ttggtcaagt
     6901 ttatgaaggt gtacttaaaa cgggagaaga agtaatcgta aaaattcaaa gaccaaatat
     6961 agagaaaata ataaaatctg atttagagat tcttagaact attgcaaata ctttaaaaga
     7021 tttaaaaaag gattttaatt tagatttagt acagatgatt gaagaatttc aaactcaatt
     7081 aatgagggaa cttgattata catttgaagc tataaatgca actaaattct ctaggatatt
     7141 taaaaatagt gatgaagtat atatacctaa agtatattca gaatataata ctaagaaaat
     7201 tttagtaatg gagaaagtta atggtacaaa gttaagtgat gtagaaaaaa taaggagact
     7261 aggttataac acaaaaacta ttgttgaaat aggcgttagg tcatttttta cacaggtatt
     7321 atctcatgga ttttttcatg cagacccaca tccaggtaat atattcgtag ttgctaaaaa
     7381 taagatagca tatatagatt ttggaatgat tggaattatt gataataaaa ctttaaacca
     7441 attaaatgaa attgcattag caggtgtaga aaagaatgtt gacaagataa tatatctatt
     7501 gatagaaatg gatgcattaa atggagaggc tgatattaag ggacttagac aggatttact
     7561 ttatctaata cattattact atgatatttc tatagaaaaa ataaatgtaa cagatatatt
     7621 aaatgaacta tttaggtttt ttagacagta taaaatagtc atgcctgcac aatttgtaac
     7681 tcttgcaaag acagttataa cattagaagg aacaagtaga actttaaaca cagatttttc
     7741 ttttggttct atgggaaaag agtttatgaa acatcattat aagagtaaat ttaaccctaa
     7801 aaatgttgtt ttaagttcaa gacaaaatgt agaagaaata ttactggaca taaaaactat
     7861 tccaaagcaa ttaaaagcaa ttttaaaaaa tatagaaaga aataatataa aaatgcaaat
     7921 tgaagatgtt aaaatgacga ggttagaaaa ttgtataata gagctaacat ctcaaatatc
     7981 tttgagttta gtgttggcat ctattatagt aggttcttct cttataattg catctccaaa
     8041 tatagaaaat aatatatgga taaaatttac tgcaatagcg ggtttcttta tatcctttat
     8101 aataggcctt tgcttagtta taagaagtat taggtcaaaa tacaaaaaag attaggggga
     8161 attaaatttg aaaaaatgga tattgaagta taaaggtaag atagaggaaa gtgaatttag
     8221 caaaaaactt aatatttccc cggaaatctg ccaaatttta aaaaatagag ggatatgtac
     8281 tgaaaaagat tcagagatat ttatgaatcc atctttagat tatttaagag accctttttt
     8341 aatgaaagat atgcaaaaag ctgttgatag aataaagtta gcagttgaga agaacgaaaa
     8401 aatttggatt tacggagatt atgatgttga tggtgtatca tctacatcta tcttgtgctt
     8461 atattttaag agtataggtt acaatgttga ttactatatt cctaatagat tagaagaagg
     8521 gtatggtata aatgaagatg ctattaagct tataagctct aggggatgtg acttgattat
     8581 aagtgttgac tgtggaatta catcagtgtc tgaggtcaat acagcgaatg atttgggcat
     8641 agatgttata attacagacc atcatgaatg tcaaagtgaa attccatcag cctttgcagt
     8701 aataaatcca aaacaagaag attgtaatta tccatttgat tcactatgtg gatgtggtgt
     8761 agcatttaaa atgattcaag ccttgactcc aaaagaagaa tttaaaacta gtatgtataa
     8821 ttatttggaa atagtaacac tagcaactat ttgtgatatt gttccactta tagatgaaaa
     8881 tagaataatt gtaaaaaatg gtttaaaatc aatgaaagaa ggcaaaaata ttggattaag
     8941 ggagcttata aaagtttgtg gtgtagaaag tgataagata ggttcttctc atataggttt
     9001 tgcaataggt cctaggataa atgcttcagg tcgattggga tattcttact taggagtaga
     9061 gctatttact actcaatcac aagaagaggc agtagaaata gctagtatat tggaagaaaa
     9121 aaataatgaa agacagatga tagaagctaa gatgtatcat gaagctgagg aaatgctaaa
     9181 gtctaatagc agatacaatg atgacaaggt gttggtttta gctaaagagg gttggcaaca
     9241 tggaataata ggaattgtag catcaaagtt aactgaaaaa tactacaaac caacaattct
     9301 tctaggtata gaaaatgggg aggctactgg ttcagcgagg tcaataaaag gatttaatat
     9361 atttgaagct ctcataaagt gcaaagactt gatgactaag tttggtggtc atgaacaagc
     9421 agcagggtta tccttggatt cagacaatgt agaaatttta gcaaatgaaa taaataaatt
     9481 tgcagattat aatttaactg aagatgatat gatagagaat gtaaatgttg agtttgaatt
     9541 acaagaaaat gtaataaatt taaatcttgt agaagaatta cataagttag aaccctttgg
     9601 attaaataat cctaatccta gatttatagt tagaaattat attttaaaag atttgaaagt
     9661 tataggtaag aatcaacaac atctgaaatt aagcatagaa aaagagaaaa gctatgaatg
     9721 tataggattt aatatgagtc atttaaaatc aatgtataaa gtaggagaca aggtagatgt
     9781 actattccaa ttagatgaaa ataactatat gggaaatagg aaagtacaat ttttacttaa
     9841 agatatacga ttggctcgac ctaaaagtgc aagtaatgac aaactttcat tgaaattaat
     9901 gtcaaaaata ataccaaaag atactcaaag cctgtataat atatctgtgt ctgattttga
     9961 attatttgat ggaaatacag atataaatat atttgattat tttgaaaaag atacactaat
    10021 aatatctaat tctataaatg gattttatag agcaatgtca gatatatcac ttatagattt
    10081 ggattttaac ataaattaca atattattga agatgttagc aaaaatactg ataaattaga
    10141 gcttatattt tctcctaata ttgataaaat agacttaaaa agatataata atattattct
    10201 ttatgattat ttatacaata aaggcgaata ttcgtatata tatgagaata aaagagaaga
    10261 aagtgaaatt ataaaatatt ataataaaac agacctatta tatttaaaga atgtagttag
    10321 taatatagtt ccatcaagag atgaatttat aacaatttat aaacaagcac tcataaaaaa
    10381 agaaattgat ttggatatgg taaatataag ggaaactttt aatgtaattc cattaaagtt
    10441 ttttacgata ttaaatgtat ttagagaatt aaatttactt gactttaatt taaattatga
    10501 aaaaaattct gtactcataa gaatattgcc aaaaccacag aaaaaattag acttaaatga
    10561 gagtttaata ctcaataatc taaaaaattt agaaaaacaa tacaatagca gttattagga
    10621 ggcgtttccc atggatttaa aaaactttat aaggaatatt gatgatttcc caaagccagg
    10681 catagatttt aaagatgtga caactctatt taaagatgga gatgcattta aatatgcagt
    10741 ggattctata gttgaagaat tgaaagataa agatgtagat ttagtaatag gaccagaagc
    10801 aagaggattt ttgatgggta cacctgtagc atatgcttta ggagtaggat ttgttccaat
    10861 tagaaaacct ggaaagttac caggtgaagt tgagagttat gagtatggat tagagtatgg
    10921 tacagatact ttagagatac ataaggatgc aattaaaaag ggtcaaaaag tagcaatagt
    10981 agatgattta ttggctactg gtggaactat ggaagccgct gccaagcttg tagaaaaatt
    11041 aggtggagaa gtagtttcta tgcaattttt gattgagcta aaattcttaa atgggagaga
    11101 aaagttgtct aactatgatg ttaattctct tataaaatac tagtactttt ataatagttg
    11161 gtataacacc aactattttc atatatatta aaattttata caaaataggt ggttatatgc
    11221 atgataaaga attacaagaa ataattgaaa aaataaaagt ttatgcacct aatgcaaatt
    11281 ttgaaatggt tgaaaaagcc tataatcttg caaaaaaagc gcatgaagga caatatagaa
    11341 aatctgggga accatacttt attcatccat tagcagttgc aaatatttta atagagatgg
    11401 aattagatat tgaaactata acagcaggtt tacttcatga tgttgtagaa gatacagaat
    11461 atacctatga agacataaaa aaagaatttg gcaaagaagt agcggatttg gttgatggag
    11521 taactaaact tggtcaaata aaatatcgtt cgaaagaaga aactcaatct gaaaatttaa
    11581 gaaaaatgtt tttggcaatg gcgaaggata taagggttat acttataaaa cttgctgata
    11641 gattgcacaa catgagaaca ttaaaataca tgcctccaga aaaagcaaag tataaagcaa
    11701 aagagacact tgaaatttat ggtggtatag ctcatagatt aggaatttcc aagataaagt
    11761 gggaactaga agacagagca ttaagattta tggacccaga agggtattat gatttagtta
    11821 gtagagtatc aatgaagaga agtcaaagag aggattatat tcaaggaatt gttgaacttc
    11881 ttaaagataa gtttaaagat ttaagtatac catgtgatgt atatggtaga ccaaagcatt
    11941 tttacagtat atatagaaag atgcaaaaaa aacataagac ttttgaagag atttttgatt
    12001 taacagcagt aaggatattg gttgataatg taaaggattg ctatgcagta cttggtatag
    12061 ttcatacatt atggagacct attccaggaa gatttaaaga ttatatagca atgccaaaac
    12121 ccaatatgta tcaatcatta cacaccactg ttgtaggtcc tgatggagaa cctttagaga
    12181 tacaaataag aactcatgag atgcataata tagctgaaaa tggtatagca gcccattgga
    12241 aatataaaga aggtatctct agcaaagaat caaaggttga agaaaaactt caatggttga
    12301 gacagatgat ggagtgggaa aaagatttaa aagacccaca agagtttatg gatgcactta
    12361 aagaagatgt atttaatagt caagtttatg tatttacgcc aaaaggagac gtaattgaat
    12421 taccagcaga atcaacacct atagattttg cctatagggt tcatacaaat gtaggcaata
    12481 aatgtgtagg agctaaaatt gatggaagaa tagttcctat tgattataag cttcaaaatg
    12541 gtaatattgt cgaggtactt acatcatcta attctaatgg accaagtagg gattggctta
    12601 atatagtgaa gaccccaaat gcaaaaagta gaattagaca atttttcaag aaggaaagaa
    12661 gagaagaaaa tgttgaccgt ggaaatacaa tattagaaag agaatttaag aagcatgata
    12721 taccacaaaa agaccctctt gttgaaaaat atatggcatt agttgctaag aaatttaatc
    12781 agccaagtgt tgaggactta attgccactg taggttatgg tggtataatg tcatctcaag
    12841 tggtatctaa ggtaaaagac ctatatttaa aagaggttaa aaaagctaaa aaggaacaac
    12901 agatacaaga agaagattta aataaacaca atataagtga aacagaatac agtaaaaaga
    12961 gaaagaaaaa cgcatcacaa ggaattatag taaaagggct tgacaatatc ttagtaagat
    13021 ttgctaagtg ttgtaatcct cttccaggag atgaaattgt aggatatatt actaagggta
    13081 ggggagtagc catacacaga gctgattgcc caaatgcaaa caataatggt gatttcttcc
    13141 aaaatagatt ggtggaggtt tcatggagtt cagcaggaaa aggtaagttt gaagcggaaa
    13201 ttcaaataaa agctattgat agaagaggaa ttgttaatga aataactcat attgttgcaa
    13261 tggataaact tagtttaaac ggaataaatg caagaaaagg taaggatagt attgtaagta
    13321 taaacttact tgtagaggtt aatgacacag aggaattaaa aattctaatg aaaaaattaa
    13381 aagcaattcc tggtgtagaa agtatttata gaatgacaaa ttagagttaa aataataggg
    13441 aggaaactat gagagcagta gtgcaaagag tatcttcatc taaagttaca gtagatgaaa
    13501 acactatagg tcaaataaat aaggggttac tagtattgtt aggagtgact catgatgata
    13561 aatcaagtga tgtagattat atgattgata aaatattgaa tctgagaatt tttgaagatg
    13621 aaaatgacaa gatgaattta tctctaatgg atataggtgg agagctttta gtagtatctc
    13681 aatttacttt atatggagat tgcagaaaag gaagaagacc tggatttagt aatgcagcta
    13741 ggccagaact tgcaaataac ctttatgaag aatttgtaaa aaaagcaaaa gataaaggtg
    13801 tgacagttgg tacaggtcaa tttgcagctc atatgatggt agagttaact aatgatggtc
    13861 cagtaacaat actattagac tcaagtaaat ctttttaagg gggaaaactt taatgattat
    13921 aaaaaaattt gtagatagtt attttggagt aaatacttat gtattaggtg atgaaaagac
    13981 taaaaaatgt gcagttatag acccaggtgg aagtttagta gagacattaa gttttgtaaa
    14041 agaaaatgaa ttaaatattg aatatattat ccttactcat gggcatggag accatatagg
    14101 atatgttaag gatattaaag aaaaaactgg tgctaaagta gttgcacatg ttgatgaaaa
    14161 agaattatta aatgacaaaa ataaaaatct aagttataca atgagatgtg gagcgcaaga
    14221 gtttgatgca gatatctatg taaatgataa agacaagctt gaacttggtg aattaaaaat
    14281 gactttcttg catactccag gacatactca aggatgtatg tgtataagag ttgaagatga
    14341 aatgttcaca ggagatactt tgtttgctgg aagtattggt agaactgatt tatatagtgg
    14401 agactttaac caaatggaaa aatcacttaa gaaattatgt aaatatgaag atgctgttag
    14461 agtatatcct ggacatggac caaccagtac tctaggggtt gaaaagaaga caaatcctta
    14521 catgtaattt tatattagta tttaaaaaag tataaaatat ttaataacta tattagtgag
    14581 ggggttcttc tactgttaaa tagaaggagt taagttgatg ttgtatgttt ttttaaaagg
    14641 acatgattat aagtatgaag tagcagaact tataaaattg tttactagtg aatttaagtt
    14701 taaagactct aatgctaaca ataatataga gtcaaagcat ttaataaata gattgattta
    14761 tgagaatggt gtattattct caagtactga atactatgaa aatggaaact taaagtacga
    14821 gtcaatacag aatatagaaa atatgaatct tgaattttta gatggatttg atagcagcaa
    14881 aacgattgaa gaatttctta atagcctaga tagtgaaagt aagaggaact ttaaaaagct
    14941 gtgtaaagaa aatatcaaaa aaagcatgtt tttagtgctt aaacaagtat ttaattctta
    15001 tgtaccatgg ggaattttga ctggtattag accagtcaaa attgtccaca atttaatgga
    15061 taaaaagcta gatgataata gcataagaac cattctaaaa gataattatt ttataataga
    15121 tgagaaaata gatttagctc ttgaaatagc taaaagagag cgattattta tgtatcctat
    15181 agataaaaat aaaatttctc tttatgtaag tattccattt tgtccaacta gatgtgtata
    15241 ttgttcattt ccttcaaatt cattaaaaca atttggacat ttaaaaagag aatatgttta
    15301 taaattgatt gaagaaatta aggggtttgc caaagttatt aaggatacta agaaagaagt
    15361 tgaaactcta tatattggtg gtggaactcc aactacactt gatgaggaag aattggattt
    15421 gcttataaat tcattgttca atgaattaga tttaagtaaa attaaagaat ttactgtaga
    15481 agcaggtaga cctgacacta ttacagaaca aaaactaaga gtgttaaaaa aacacaatgt
    15541 tagtagaatt agtataaatc ctcaaactat gaatgatgat accttggtaa agattggaag
    15601 agagcataga gtatctgatt tagtagattg ctttaatatg gctcgtagaa tgggatttga
    15661 taatataaat atggatataa ttttaggtct tatagatgaa gacttaaata tggtaaagaa
    15721 cacattggag gagataaaaa aactttctcc tgaaagcctt acagttcata cattagccat
    15781 aaagagagca tctaacttaa atataaatat ggataggtat aaagaacatc taacacaata
    15841 tgaagaaatg gtaaaaatga tagatttatc aatgaaatat gcaaaagata tgggattaaa
    15901 tccatattat atgtacagac aaaaacatat gttgggaaat ttagaaaata taggttatgc
    15961 taaagaagga tatgagtgca tttataatat acagattatg gaagaaaaac aaagtaacta
    16021 tgcattaggt gcaggtgcta tatcgaaatt tgtctatgta gatgaagata gaattgaaag
    16081 agtagaaaat gtaaaaaatg ttgaacaata cattgaaaga gtagatgaaa tgataaaaag
    16141 gaagaaagaa gaggtatata aaaatgttga ctaaagctcc tagaggaact aaagacataa
    16201 ctccaaaaga ggcttacaaa tggcgttatg tagaaaataa atttagagaa atatgtgctt
    16261 tatatggata tgaagaaatg gtaacaccta tatttgaaca tacagaactt ttcaagagaa
    16321 gtgttggaga tactacagat atagttcaaa aagaaatgta ttcttttaaa gataaaggtg
    16381 atagagaaat taccctaaag ccagaaggta cagcaggtgt agtaagagca tttatagaaa
    16441 ataaattata tgcagatact caaccaacaa aattatttta tgtgacacct tgttttagat
    16501 atgaaagacc acaagctggt agacaaagac aatttcacca atttggaata gaagcacttg
    16561 gaagtgatac tccttcaatg gatgctgaaa taatcgcact agctgttcaa ttttttaatg
    16621 aagttggatt aaatgatttg gtagtgagta taaattcagt tggctgtcca gtttgtagaa
    16681 aagagtacaa tgctttatta aaagagtatc ttgactcaaa agctgatata ttgtgtgata
    16741 cttgcaatga gagaagagaa aaaaatccaa tgagggttat agactgtaaa aatcctacat
    16801 gtaaagaaaa tatcaaagat ataccattta ttgcagacca tttgtgtgat gattgtaaat
    16861 ctcactttga taaacttcaa gaatacttaa aggaaatgaa tataaacttt gtaattgata
    16921 aaacaatagt tagaggctta gattactata gaaaaactgc ttttgaaatt atatctaatg
    16981 atataggagc tcaaagtact gtatgtggtg gaggaagata tgatggtctt gttgaacaat
    17041 taggaggacc aaaaggtata agtggaattg ggtttggact aggaattgaa agacttttac
    17101 ttacacttga aggtaatgga attgaaatag aaaaccctca atctacggat atatttatag
    17161 taactatagg tgaagaagca aatacaagaa gctttaaatt gttaaaagac ttgagacaaa
    17221 atcatataag tgcagataaa gaccatatag agagaagtgt taaagcacaa tttaaatatt
    17281 cagataaaat aaattcaaaa tttacaatag taattggaga tgatgaactt aaaaatgaca
    17341 ctgcgacatt aaaaaatatg aaaacatcag agcaaactac tgtaaaatta agtacattag
    17401 ttgaagaatt aaagcaaaag ctgtaaaata agaaagtatg atgttttgtg tattagataa
    17461 atatatagca ttaaaattaa ataaaatgga ggtagtatct tggaaacttt aaagggttta
    17521 aagagaactc actactgtgg agagttgaga gaaaaaaaca taaatgaaga agtcgtactt
    17581 atgggatggg tacagaaaaa aagaaattta ggtggtcttg tatttgttga tttaagagat
    17641 acaagtggat tatgtcaaat agtttttgat acagatgtag ataaagaagc ttttgaaaaa
    17701 gctgaaaaat taggggctga atttgtaata gctgtaaaag gaaaagtatg cgaaagacaa
    17761 tctaaaaacc ctaatatgcc aactggtgat atagaaatat ttgcaactga acttagactt
    17821 ttaaacaagt cagaaactcc gcctatttat ataaaagatg atgatgatgt ttctgaagca
    17881 ctaagattaa aatatagata cttagattta agaaaaccat ctatgcaaag aaatttaaaa
    17941 ttaagacata aagtaatgaa tattacaaga aattacttat caaacaatag attttgcgaa
    18001 atagaaacac catttttaat agctccaaca ccagaaggag caagagatta tctagtacca
    18061 agtagagtta atccaggaaa attttacgca ttaccacaat caccacaatt atataaacaa
    18121 ttattgatgg taagtggtat ggatagatat ttccaaatag ttaaatgctt tagagatgaa
    18181 gacttaagag cagatagaca accagagttt acacaaatag actgcgaaat gtcttttgtt
    18241 gagcaagaag atgttatgag tatgattgaa ggtttgcttg aagcgatatt taaagaagtt
    18301 ttagatgtag aattggcact tccacttcct aagatgactt atgcagaagc tatgtctaaa
    18361 tatggttcag ataaaccaga tactagattt gggtatgagt taacagatat atctgatgtt
    18421 gtttgtaact gtggatttaa agtgtttgct gatgctacac aaccaggaaa aagtgttaga
    18481 ggtataaacg taaaaggcaa agctgacgac tttactagaa aacaaatatc atcactagaa
    18541 gaacatgcta agacatatag agcaaaaggt cttgcttgga tgaaagtagg acaagaagga
    18601 gtaacttctc caatagcaaa attcttcaat gaagaagaaa tgaacgctat acttacaaga
    18661 atgaatgctg aagtaggaga tttactttta tttgtagctg ataaaaactc aatagtattt
    18721 gatgctttag gacaagttag acttgaagta gcaaataggc ttaatctatt ggataaaaat
    18781 gtatataact tactatgggt aactgagttc ccagtttttg aagaagatga agaaacaggc
    18841 acattctcag caatgcatca cccatttaca tcacctatgg atgaagattt agacaagtta
    18901 gaagaaggtg ataagtcatc tttaagagcg aaagcttatg atattgtatt gaatggttat
    18961 gaaataggtg gaggaagtgt tagaatatca aattctgatg ttcaatcaag aatgtttaaa
    19021 gcacttggtt ttacagagga aagagctaat gaaaagtttg gttatttatt agaagcattt
    19081 aaatatggta ctcctcctca tgctggtcta gcttttggtc ttgatagact tgttatgtta
    19141 cttgcaggaa ctgataatat aagagaagtt attgcattcc ctaaaaatca aaatgctgtt
    19201 tgccctatga caaatgctcc tacattggcg gaagatgagc aattagaaga attaagtata
    19261 aaagttgata taaaagataa tgaataatat gtagttgcaa gaaactaaaa tgtaaaaagt
    19321 ctggatataa aatatattta tattcagact ttttttatta gaattatttt atattttttc
    19381 tctatatatt ttcataaatt atacttatat tcttaaccaa tataattaaa atactatgtt
    19441 taaaaatttt acataaactt ggttgtttta gaaactactt ctgtaacgag tacacgattt
    19501 taattaaaga atatataaca aatttgaaat aagtctaata tatgacttaa tagattaaat
    19561 taaaataata aatttggact atttatctat ttataactta aattatatca aattgataac
    19621 aaactagata ttatactttt ttgtagaata gaattacaat tatataaaga aaaattctat
    19681 taatagtgaa aaaaaagtag aaaaaagtag aaattattct tttataaaga aagggaggga
    19741 taatattgca agagagagaa gaattagttt taaatccagt tccaaaagag gaaagagttg
    19801 gatggttagc tccattgttt aatatgcttg gttctaacat agctatatct gaactaatgg
    19861 ttgggggaac tttaatttta ggaatgactc tttctaatat gattattaca tcaattatag
    19921 ggaacttaat attagttgca attattatga tacaaggtta tataggttac aaagaagggt
    19981 taaatacgta tgtattagct aagggggcat ttggagagat aggaggaaag tatcttatat
    20041 ctctattact tggtataact agttttggat ggtttggtgt acaagcaggg gttgcaggac
    20101 tatcagtaca aaagatattc ccaagtgtaa acctaacatt agttacagtt atacttggat
    20161 tatttatggt tgtttttgca ctgtatggtt ttaaagctat ggctaaattt aattacttag
    20221 ttataccacc acttatgata ttaatggtgt ggggagtttt taaagctttt tctacttatg
    20281 gagttgaagc tatttataat tatactcctc aaacaactat gagtatggtt gaaggtctta
    20341 atattgtagt aggtcttgtt atagttggtg caattatatc acctgaccag ttaagatata
    20401 cgagacgagt aaaagacatt tggattatca gttttttagg tctagggatt atttcattat
    20461 tccaacaagt tgcagctggt gtaatgtcta tgggagcacc tacttgggat ataacagaag
    20521 tattggctaa tttaggtttt ggatgggtag cgtttgttat tttaatactt gcatcttggt
    20581 ctacaaacgt atcaaatgct tattctggtg gattagcatt aaaaactatt tttccaaatg
    20641 taaaaagaaa tgttttgact ttagttgcag gtctaatagg tacaataata gcagctactg
    20701 gtataatatt taaatttcag tcatttttat ctttcttggg aatcgcagtt cctgcaatag
    20761 caggaattat gtggtgtgag tattatttta tacaaggaag aacttataag catagagaag
    20821 gaataaattg gattgcagta ataagttggt taataggttt tgcagcgtcg tattatagtt
    20881 caaaaataaa tttcttaata cctccgataa atggaattgt agtgagtatg gttatttatt
    20941 atatattaat gaattgtttt ggaatcaaag ataaataaag ggggcaatta catgggtcgt
    21001 aaggtgaaaa taggtataat tcaacaacac agtgtacttg gaaatgttaa aaaaaatata
    21061 gaaaaagcag tagagatgat agatgactta ggaaaacaag gggcagatat aatttgttta
    21121 ccagaactat ttgctacagg atataattta gagtctttag gtggagttaa aacattagaa
    21181 ttaataagag aacataataa gtacatagag gaatcaatgt cagaagctgc taaaagaaat
    21241 aatgtatacc tgatatctcc atatggaacc ttagaaaaag ggtctacaca tgtttataat
    21301 tcagcagtta tatttgatag aaaaggtaag attatgggag aatactgtaa aaatcatctg
    21361 tggtcattag aagcagtata ctttaaagga ggggaaaaag tagaggtata tgatgctgat
    21421 tttggtagat ttggtgtaat gatatgttat gatgcaggtt ttccagaagt atccagagaa
    21481 ttgacattaa aaggttctga aataatattt ataccatctg catggagaat acaagatgaa
    21541 gatatgtggg atttaaatgt atctcaaaga gctttagaga acactgtata tacagtaggt
    21601 gtaaatttag taagtaatga ctctaattta atactatttg gaaagagtaa aatatgcaat
    21661 cctagaggta ctgtaataac tcaattagat acatacaggg aaaaatatgt attatcagag
    21721 attgatttag atgaaataaa aaagtatagg gaagatatcc cctatttgaa agacagaaaa
    21781 agtagctata acatataatt ctatactata atacaagttc aatataaaat gataaaaaga
    21841 ggatgtatca atattaagat taaatttatt atactttgat atatcctctt taatattatc
    21901 aactttgatt taaatatttt taaaatagtt gataagaaag tttttgaaca acaacacagc
    21961 tttagattca tagttttcgc taaaccatga aagatttaaa tatcttttaa attcaatatc
    22021 tttgatatgt aacaacttaa tctcagggtc ttgagataat ccccatgatt ttccacatat
    22081 aaatccaact ccttgaagtg attttataag agcataaatt gtatatggtg aatcagactc
    22141 aaatgctatt ttgggtttga aattagctga ttcgcacagg atatcaatta cctctctata
    22201 attctcaccc ttcgttataa ctataaagtt ttcatctgaa acttcgctta gatatacttc
    22261 atcctttaat gataaaggat ggtttattga aactcctaat agtatttctt cgcatgttaa
    22321 agttatgcta ttttctgagt ttagctttgt aaatgaagaa gatatgtaca aatcaaaatc
    22381 acttttttta taagatgatg gcaaagtatg tgacacatta aaagtaatat tgggatatag
    22441 ttttctgaaa ttactcaaaa gtgatggtaa cacattagcc gctgcaggag ataatatctt
    22501 tacttctcct gaattttcca aagaagtgtc ttgtaactcc ttttttgaac tttctactaa
    22561 atctaaaatg ctatctactc tttttagaaa caatcttcca tactcattta aaacaatata
    22621 tcttcctttt ctgtcaaata actgaactcc aagttctttt tctaaggaag atatagtttt
    22681 gcttaaagct ggctgtgcta tatttaactt gtgtgatgct tttgttatgt gctccattct
    22741 agcaacagtt tgaaaatact taagatgcaa taaatccata gtataaaata cacctctttt
    22801 aatatgactt gagttatatc aattttataa tcaactcc
//
LOCUS       DAMJYX010000056        22242 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJYX010000056 DAMJYX010000000
VERSION     DAMJYX010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.056"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            143..1327
                     /locus_tag="PC479_002242"
     CDS             143..1327
                     /locus_tag="PC479_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804783.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1704..2813)
                     /locus_tag="PC479_002243"
     CDS             complement(1704..2813)
                     /locus_tag="PC479_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2804784.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3276..4469
                     /gene="nifV"
                     /locus_tag="PC479_002244"
     CDS             3276..4469
                     /gene="nifV"
                     /locus_tag="PC479_002244"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2804785.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4494..6419
                     /locus_tag="PC479_002245"
     CDS             4494..6419
                     /locus_tag="PC479_002245"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2804786.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6441..7436
                     /locus_tag="PC479_002246"
     CDS             6441..7436
                     /locus_tag="PC479_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2804787.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7761..8234
                     /locus_tag="PC479_002247"
     CDS             7761..8234
                     /locus_tag="PC479_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804788.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8268..8762
                     /locus_tag="PC479_002248"
     CDS             8268..8762
                     /locus_tag="PC479_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2804789.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8859..9380
                     /locus_tag="PC479_002249"
     CDS             8859..9380
                     /locus_tag="PC479_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2804790.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            9569..9880
                     /locus_tag="PC479_002250"
     CDS             9569..9880
                     /locus_tag="PC479_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2804791.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            9908..10594
                     /locus_tag="PC479_002251"
     CDS             9908..10594
                     /locus_tag="PC479_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2804792.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            10826..11710
                     /locus_tag="PC479_002252"
     CDS             10826..11710
                     /locus_tag="PC479_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2804793.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            11809..12984
                     /locus_tag="PC479_002253"
     CDS             11809..12984
                     /locus_tag="PC479_002253"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2804794.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            13112..13966
                     /locus_tag="PC479_002254"
     CDS             13112..13966
                     /locus_tag="PC479_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804795.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            14010..15245
                     /locus_tag="PC479_002255"
     CDS             14010..15245
                     /locus_tag="PC479_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2804796.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            15346..16287
                     /locus_tag="PC479_002256"
     CDS             15346..16287
                     /locus_tag="PC479_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2804797.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(16506..16859)
                     /locus_tag="PC479_002257"
     CDS             complement(16506..16859)
                     /locus_tag="PC479_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2804798.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            17155..18066
                     /locus_tag="PC479_002258"
     CDS             17155..18066
                     /locus_tag="PC479_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2804799.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            18326..18760
                     /locus_tag="PC479_002259"
     CDS             18326..18760
                     /locus_tag="PC479_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804800.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            18783..20318
                     /locus_tag="PC479_002260"
     CDS             18783..20318
                     /locus_tag="PC479_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804801.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            20402..21769
                     /locus_tag="PC479_002261"
     CDS             20402..21769
                     /locus_tag="PC479_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2804802.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            21804..22028
                     /locus_tag="PC479_002262"
     CDS             21804..22028
                     /locus_tag="PC479_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2804803.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         8688 a         2228 c         4070 g         7256 t
ORIGIN      
        1 atttaatata aaggtggaaa taagttgaaa atgtaaagtc attggaaaaa tatagttgaa
       61 tttaatgagc aaaaatgtta ttgttttatc aataaagtgt taaaataaaa actttcaata
      121 tcattttagg gaggatgatt tcatgcaaaa aagtgtaaag aaagcaaaag taacgggctc
      181 tatgttagct atttttggtg ttgcaagcgt tctttttagt tcacatgcag gaggaggatt
      241 tgctacaggg aatcaagaaa ctcagtacta tgtgcagtat ggatggacag caccacttat
      301 ggcaatttta gcaatgatta tacttactgc taccatgaga gaagttatca taatgtataa
      361 taataacaat tgtaggaatt ataaggattt attttgtgag ctttggagac catatccaaa
      421 acttgaaata atatgggaga tatattacta tctaatggtt ttaatagctg taagtgctgt
      481 tatagcaggt gcagcagcag tatttcaaag tataggagta aattattttg tagctgtatt
      541 tattattgga gttgtacttc ttgtctttac tatatttggt gctatgcttg tatctaaagc
      601 agctacagca atgacaatag ctattctagt atgtactctt actattttta ttgttggtat
      661 aaaagctaaa gtaccagaaa taactgaaat attatcaaat agaacgagtt tcacacctgg
      721 ctatatcaag ccaatactta atacttttat ttatgcaggt tttcaatcgg ttgttatacc
      781 tacacttgca ggatgttcaa gaccacttct taagaactca aaagaagcta ctaaggcaat
      841 gataatttca tttgttatga atgcaattgc attaggactt gctgtaacta tgctaatggg
      901 atggtataga gaaattattg ctgctgggca aacaacactc cctactcttt atgttgcagg
      961 tcaatctgga aatcatacta tatacataat ttataatgtt gcattattcc tttgtttaat
     1021 gtctacagga gtaactacta tatttggtct tgtaaataga tttgaagacc ataaagcact
     1081 ttctttcctt tcttcaagaa tgaaaagaag ggtatttaca gcttgtgcaa taatggtagt
     1141 gtcaatgctt atatcactta caggattaag taatattgtt aaatatggat atggatattg
     1201 tggttatctt ggtctattca ctatagtaat tccatttctt acactaggac attataagaa
     1261 taagaagttt gcaaaggaaa atccagaagc taaatggcca gcggaattaa atgaaaatgt
     1321 aaattagatg tagtagcaaa aaatattgct aaagagaata ctataaataa gaatactata
     1381 gataatatat ctgcatgttt attaagatta tttgcaaaat ctaatattaa acaactataa
     1441 acaagcaata taaaactaag aataaaagat aaaaagaggg tatctcaaaa tggactttta
     1501 gtttataagg cactcttttt tatatgaagt caaatatatt ttcattattt tgacaataaa
     1561 agagactaat cctactttgg atagcctctt ttttctttaa tataaaatag tataacagca
     1621 aagtatagat tgatacatgt tttctgtagc tttctatata ttacagtgtt tgtgtggcat
     1681 tggtatttta agcaatctat aaattataat tctataattc ttctactaat tttagattct
     1741 atagccgtct ctatttcact taacattcgt tcatttttag ggtcaataaa agcacatgta
     1801 tagccttctt caccagctct accagttcta ccaatacggt ggatgtagtc ttctggtgtt
     1861 tctggtaaat cgtaattata tatatgactt actccagata tatcaagacc acgagaagct
     1921 acatctgtag ctattaagta ttgtatatct aaatttctaa atgatttcat tattctttct
     1981 cgttttgatt gagcaatatc actatgaagt ttttgacaat tatatcctct tatagcaaga
     2041 gcttcttcaa gattgtcaac ccttctctta gttctacaaa atattattgc cataaaagga
     2101 ttatcttcat ctagtacctt acataaggca tctaattttt ttctatctgt agtttctact
     2161 acttcttgtt ttatattatt aagagtgatt tcttcttttt gaatagaaac tacaacagga
     2221 ttttttgtgt atctataagc taattttttt acttgagaat ttatagttgc agaaaagcat
     2281 agtgtttgct ttttgtttga agtctctttt aaaatagctt ccacttcatt tttaaatccc
     2341 ataagcaaca tttggtctgc ttcatctaaa acaaaagtct ttaacttact aaggtctatt
     2401 gtttttctat ttagatggtc taaaagccta cctggtgtag ctataatcat atgaatattt
     2461 ccttttagtt tctttatttg agaaccaata tcttttcctc catatgctgc aagaatactt
     2521 acatctttag attctttaag tttcatagct tcttctgtta tttgaatagc tagttctctt
     2581 gtaggagaaa gtataagtac ttgtatatca tttatatcta atgatatatt ttcaaatatc
     2641 ggaagtaaaa aagctagtgt ttttcctgtt ccagtttgag cttctgctat tacatctttt
     2701 ccttctttta ttagtttaat actttctttt tgtataggtg taggtgttgt tattcctgat
     2761 ttttttagaa tatttattgt attttcacta atccctaagc ttttgaaatc cattgttatc
     2821 ttacctttca tgaaaaattt tcttttttat taaccaatta ataatatcat atttgtaagt
     2881 atttttagta aattttttaa ttattattta tatagcttaa aaatatatta agatacttta
     2941 aaatggaatg taacttaaat taaattttag taggaatttt ataggtttat ttttaaaggt
     3001 tttttgaaaa taaatctatt taaaaattaa attaaaatgc aagaaaaaca taataacttt
     3061 catttttaag aagttttcac aaaaaagaaa aaatatagtg caataaatga tacaaagtaa
     3121 tataatagat tacaagatat gaaaagctaa aaaaataaat tgcaaatgaa attaaaatga
     3181 ataaaaaata aaaaaataat cataagttag aagtatcaat gaaaaataag acttaatgtt
     3241 tttaaaatta aaagtatact aaggggggca ttaatatgtg cgttatatca aaagatagag
     3301 caaaggaaat aaaaatagta gatactactt taagagatgg agagcaaaca gctggggttg
     3361 tatttgcaaa tagagaaaaa ataatgattg ctgaaatgtt aagtgaccta ggtgttgacc
     3421 aaattgaagt tggtatacct actatgggtg gagatgaaaa aaatgtaata aagcacatat
     3481 gtagtagaaa tttaaaatca gatataatgg cttggaatag agcagtaata aaagatgttg
     3541 aagaatctat ttcttgtggt gttgatgcag tagctatatc aatatcagta tctgacattc
     3601 atatagaaaa taaattgaga acttctagag gctgggtttt agaaaatatg gctaaaacag
     3661 ttgaatttgc aaagaaaaat ggattatatg tttcagtaaa tggagaagat gcttcaagag
     3721 cagatataga cttcttaact gaatttataa atgttggaaa acaagcaggg gcagatagat
     3781 ttagatactg tgatacagta ggagttatga atcctttttc tataaagaat gcaatagaaa
     3841 ctctttatga aagaactaat tttgatatag aaatgcatac acataatgac tttgggatgg
     3901 caactgcaaa tgcacttgca ggaatagcag caggagcaaa ttatgttggt gttacagtaa
     3961 atggattagg tgaaagagca ggaaatgcag cattagaaga agttttaatg gcacttaagt
     4021 gtgtttataa atgtgattta aataatatag atactagaaa atttagagga atatgtgagt
     4081 atgtagcaca ggcatcagga agaatacttc ctacatggaa accagtagtt ggggataata
     4141 tgtttataca tgaatctggt atacatgcag atggagcttt aaaagaccca cataactatg
     4201 aaccatttga cccaagtgaa gtaaatcttg aaagaaaaat agtaataggt aaacattcag
     4261 gaagagctgc agttgtaaat aagttgagtg aatatgaaat gtatatttct ccagaaaacg
     4321 ctaccaagct attaaatgct ataagagcta catctataag attaaaaaga agtttaatgg
     4381 ataaagaaat tcttcaatta tattgtgata tactagcaca tgagaaaggc acaacagaag
     4441 aagaagcagt tagaggttca tatatttaag atataattgt ttaggaggaa aatatgggag
     4501 ataatatagt ttataaaata attaaaaaac atatagtaga tggagaagct gttgctggaa
     4561 gttcaattgg tataaaaata gaccaaactt taacacaaga ttctactgga acaatgacat
     4621 atcttcaact agaagctatg ggaatagata aagtaaagac taaaagaagt gtggcatttg
     4681 tagaccataa tatgttacaa caaggatttg aaaatgcaga cgaccataag tatatacaaa
     4741 cagtggcaga taaatatgga gtttatttct ctaagccagg aaatggtatt tgtcatcaag
     4801 tatttttaga aagattttca acaccaggag atacactttt aggttctgat agccacacac
     4861 ctactgctgg aggtgtgggc atgatggcta ttggagctgg tggattagat gttgctttag
     4921 ccatggcagg aggagcatac tatataaaag caccaaaagt ctgtaaggtg aatctagttg
     4981 gaaaattaaa taatatggta tcatcaaaag atataatatt agaagtgtta agaaaacaaa
     5041 ctgtaaaagg tggagtaggt aaagtttatg aatatggtgg agaaggtgtc aaaagcctat
     5101 ctgttccaca aagagctaca ataacaaata tgggggctga acttggagca actacttcaa
     5161 tcttcccaag tgatgaaaaa acactagaat tttttaagag tcaaggaaga gaagacgcat
     5221 gggtagagct aaaaccagat gcagatgcag tttatgatga agagattact ataaacttgg
     5281 atgaattaaa accattagct gcaaaacctc atagcccaga caatgtagat gaagttgaaa
     5341 atataggcaa gattaaaata gaccaagtgg caataggaag ttgtacaaat tcttcttatg
     5401 aagacttaat gaaagtagca caaatcctga aaggaaataa agtacataag gatgtgagtt
     5461 tggtaatagc accaggttca agacaagtta tggagatgat tgctagaaat ggggctttgg
     5521 cagatataat aagtgctgga gctagaatac tagaaaattc ttgtggtcca tgtattggta
     5581 tgggtcaatc tccaggtact gactctgtgt ctttaagaac tttcaataga aatttctatg
     5641 gtagaagtgg gactttgtca gcacaagtat atttagtaag tccagaagtg gcagcagtaa
     5701 gtgcaatcaa gggagtgctt acagacccaa gagaatttga tattaaattt acaaatttag
     5761 atgtaaatga gtttttgata gatgatagca tgataattaa acctgctgat gtaggaagtg
     5821 atgtagaagt agttagagga cctaatataa aacctttccc tcttaataca gagctaagtc
     5881 agtctatagg tggaaaagtt atattaaaga cagaagataa tataactact gaccatataa
     5941 tgccatcaaa tgctaagtta cttcctttta gaagtaatat accgtatctt gcaaactatt
     6001 gtttcaatac tgtagataca gaatttcctc agagagcaaa agacaataat ggtggattta
     6061 tagttggtgg agataattat ggtcaaggtt caagtagaga acatgcagct ttagcaccat
     6121 tatatctagg tgttaaaggg gtaatagtta agagctttgc aagaatccac aaggctaact
     6181 tgataaatag tggtataatt ccaatggagt tttgtgatga gaaagattat gaaaatatat
     6241 ctcttttaga taatctggag ataccaaata ttttagacaa tctaggtagt ggaattttag
     6301 aagttaagaa tacaactaaa ggaacttctt ttaaggtcaa agtagaattg tcagcaaaag
     6361 aagttgatgt attaaaagct ggtggaaaat taaactacac taaaaatcaa gctaattaat
     6421 atgaaaagag ggaatattga atgtataaag tgacacttat accaggagat ggaataggac
     6481 cagaagttgc gaaagctatg aaaaaggtag tagaagctac tggagtagaa atagaatggg
     6541 aagaagttaa tgcaggagag gcagttattg aagagtatgg tactccactt cctgaatata
     6601 ttatagatag tataaaaaag aacaaaatag ctataaaagg cccaattact actcctgtag
     6661 gtaaaggatt taggagtgta aatgtaactt taagacaggc attagatttg tatgtcaact
     6721 taagacctat taaaagtttt aaggggataa aatcaagata tgaagatgtt gatttagtgg
     6781 ttgtaagaga aaatacagaa gatttatatg ctggaataga acataaaata ggtgattatg
     6841 cagctgaaag tataaagata ataacaagaa gtgcaagtga aagaatcgtt gattttgcat
     6901 gtaactatgt taaagataat aagagaaaaa aagttacagc tatacataag gcaaatataa
     6961 tgaaaatgtc tgatggattg tttttagatg tgtttagaga agttgcatca aaacatggtg
     7021 tagaatatga tgatttgatt gtagatgcag cagctatgaa cttagtttta aatccagaaa
     7081 attatgatgt aatggtaatg ccaaatcttt atggagatat actatcagat ttaggtgctg
     7141 ggcttgttgg aggtttaggt ataattccaa gtgcaaatat tggtaaagat tgtgcgatat
     7201 ttgaagctgt tcatggaagt gctccacaaa tagcaggtca aaataaggct aatccaacag
     7261 cacttattca atcatcagta atgatgctta gatacttagg tgaatatgaa aatgcacaaa
     7321 aaatagaaac agctttagaa aaagtatttc ttgaaggaag taaattgact gtagatttag
     7381 gtggctcagc ttctactaca gagtttgcgg atgaggtgtg taagtatatt gtatagattt
     7441 tatttaggga aaaagtaaaa tatagattga agaaaagagg ctttctcaaa atagagataa
     7501 gcctttttta ttggaatgat aaaatatatt tgattttata caaaagagag ttcctcataa
     7561 acgaaaaagt taattttggg atatccattt taattttaaa attaattgat taatacgcaa
     7621 agaaagatta aataaattta ttatttttaa ttgaactttc aaccaaaata tggtaaaatt
     7681 taaaaagttg aactttcaac taaaaatgaa aatattgatt atgtgaaatt ctataaaaca
     7741 gaaactattt gactttagcc atggtagttt cagtggaggt gtgtttgatt aagactttag
     7801 atagtaatat attaagagaa gtaggtactt tatctagagc agtaaattca ataaatgata
     7861 ttaagtacaa ggaattaaag ttacaaaaag gacaatttac atttttgaca agaatctgtg
     7921 aaaatcctgg tataaatctt gtggaattat caaatatgtt aaaggtagac aaggctacaa
     7981 caaccaaggc tatacaaaaa ttaataaagg caggatatgt agataaaaag caggataagt
     8041 ttgataaaag aggatataat ttaactccaa ctgataaatc tttggaagtt tatgagttga
     8101 taattgaaga agagaataga agtatagaga tatgttttga taattttaca gatgaagaaa
     8161 aacaagtggt aactaagtta ttagaaaaaa tgagcaaaaa cgtagaaaat gaatggttta
     8221 aagtaaagag atagtaaata aatacattaa cttaaggaga tatttttatg attagaaaag
     8281 cgaatatgaa tgatttagaa agcattatga aaattataaa atctacagtg gaagaaatga
     8341 agacttataa taatacacaa tgggatgaaa actatccatt agaaaaagac tttgtttcag
     8401 atattaagaa acaagattta tatatttatg aagtggatgg agaagtagca gggtttatat
     8461 gtcttaatta tgaagagcca gaagaatatt taggcttaaa ttggtcatct aataaaaaag
     8521 caatggtaat acatagaatg gctgtaaatc caaactttag aaagacagga atagcatcta
     8581 agttagttga ttttgctgaa aaattagctg tagaaaacaa tgtatcatac ttaaagagcg
     8641 atacatattc aataaactca aaaatgaatt tattacttac aaagtgtggt tttataaaaa
     8701 tcggagaaat gagtttttta ggaaaggaga aatctttcta ctgttatgat aaaatattat
     8761 aaagagcatt tataatacaa ggggtaaata atagattttc agcattagta agggcataat
     8821 ataacctagt atatgctatt atgaaaggga tgatttatat gattggattt ctaaagaaaa
     8881 gaagaagtat tagaaaatac aaggatgttg aagtagaaaa agaaaaatta gataaaatat
     8941 taaaagctgc tcttttagct ccatcatcaa aggggcttag aacatgggaa tttatagtgg
     9001 tagatgataa agaaaagcta attaatttat ctcaatgtag aactaaaggt ggtggatttt
     9061 tcttaaaaaa tgcaccttta gctattgtaa ttattgcaga taaagaaaaa aatgatgttt
     9121 ggatagagga tgcaagtata gctgcttcat acatacaact tcaagctcat gaactaggtt
     9181 taggttcttg ttggatacaa gtaagaaata gaatgcatga tgataatata gaagctgata
     9241 agtacattag agaagaatta aaagtaccta gcaagtacag tgtagaatgt ataatttcta
     9301 taggatatcc tgatgaagaa aagaaagcat acggtgactc tgatttagac tataaaaagg
     9361 ttcattttaa caacttttag tatataatat gttgtattaa gacattatat ttttttctat
     9421 tgttaatata tacttaaaat tgtaaaaata tatctgaatt ttactaactt atctaggggt
     9481 tatgtacaag gattttaaag ataaatatag aaattaatgt ttataatcga tacataactt
     9541 gagtaacaaa atttaaggag gttaaagtat gggtatattt aaaacaaaaa tggatgagga
     9601 ctggaaagta aactacatta aagagtttaa tgaaatgaga gattcttatg agagtaaact
     9661 tcaaaaaaaa cagtttgagg tagatagttt gaaatcagaa ttggatagac taagaagcta
     9721 taaaaatagc cttaagccaa aagaaaaaca aattactgat gatgatataa ataatataaa
     9781 gagtttaaga agagatggct taagctataa agaaatttca aatcaaacaa gttggagcaa
     9841 agcaactgta agtagggtat taaatggact ttacgattag catatacatg gggtgattta
     9901 aattgggatg aagtatagtt acaaaaaatg cataatagat tctattttat tgatgtttat
     9961 agttcaaata ttaaggatga ttttgaatta tgtacttcta agtcaatttg aatttactct
    10021 tgaaaatttt aatattataa acttaatttc ttttacatta gttggtttat cgttaatttt
    10081 atttttaaaa gataattctt tgtataacaa ggttagaaat agaaaaatca cagaagcttt
    10141 tgaagaaaat aaaaacaata tacttattga aaagtgtaag ctgatacttt ttgttgtggt
    10201 tttatcttta gcaataattg taacttattg tactaaggga tatgtacttt ttaatgtaac
    10261 tatgatgact ttgtctgtat tgatagtccc aatatttgaa gagttatttt ttagagaata
    10321 tatatggaac tatctaagta attttataaa gtcaaagagt aaaattatct gtataacatc
    10381 tatactatct ggaatttata atataggata catagatgtt ataagaaatt atgtaatatt
    10441 gtataacaat tcaagctaca cctttgaagt tattatttca aaaattatga taggtactgt
    10501 ttttggtatt gtacttggat tagttaagta taggtttaga gatgtagggt tttgtatatt
    10561 attgaggagc ttatttgcta tatttattag gtagatagta taatcggttt ataaggttgt
    10621 tttaaattta attttaaatc aaccttattt tatttgtgtt ttataaagca cttatatatt
    10681 aataactaat aaatttaata aaaattattt gaatattttt aaaatatata aatatgtatt
    10741 gacatataaa tattatgaat tacaatttat ataaactaat aaaaattaaa taatattaat
    10801 caacttattg aatggaggaa taaaaatgaa atttgtgact ttttgtagta gcaatgaaga
    10861 aaaaataggt gtatttaatt cagaaacaaa tagtatatat gaaattaata gtttaggatt
    10921 aagtaaatta tatactgata tgaatgattt tattgaaaac gtatctacag gagatttaga
    10981 aaaaataaaa aacaattcat ttgaaaacgc aaagtgttat aagttagaag aagttaaatt
    11041 atgttcccca attgtaagac ctaaaaaaga tattatatgt ttaggtctaa actataaaga
    11101 ccatgtcaat gaaatcccag atggagttat aaaaaacgta gttatgccag attatccaat
    11161 ttatttttct aagagagcag acaaaataat tggagtggat gataagataa gcttacatgg
    11221 agatttggta gagaagttgg actatgaatc agaacttgca gtaatcatag gtaaagaagg
    11281 tataaatata tcaaaagaag atgcttatga atatatattt ggatatacaa tagtaaatga
    11341 tataagtgaa agagcacttc aagataaaca tgttcaatgg tttagaggaa aaagcttaga
    11401 cacacataca tctatgggac cttgtatagt acataaagaa gaatttgaac atcctttaaa
    11461 attagatata agtagtgtag taaatggaga agtaagacaa gattctaata cagagtactt
    11521 tatatttgat atacctacta taataaatga tttatcaaga ggaatgactc taaagccagg
    11581 agatataatt tcaactggaa ctccagcagg ggttgctatg gggatgaatc ctcaagtata
    11641 tttaaagcat ggcgatatag ttgaatgtaa agtcgaagga ataggagttt taaaaaatat
    11701 agttgattaa tggataaata tgccatataa ttttattagt acgaataatt taattaatat
    11761 aggattaatc aaacttatga ttaatttatt atagagaggg atgagtatat ggcaagagaa
    11821 ctagaattaa agtatggctg taatccaaat caaaaaccat caaagattta tatgaagaat
    11881 ggagaacttc ctatagaggt attaaatgga aaacctggat acataaattt tttagatgca
    11941 tttaatagtt ggcaacttgt gaaggaatta aaagaagcta caggtcttcc agcagcaact
    12001 tcatttaaac atgtaagtcc agcaggagca gcagtagggg tacctttatc agatacttta
    12061 aagcaaatat attttgttga tgatttagag ctttctccat tagcttgtgc ttatgcaatg
    12121 gcaagaggag cagatagaat gtcttcttat ggcgatttta tagcactttc agatgtatgt
    12181 gataaggaga ctgcaactat tatagcaaga gaggtttctg atggtataat agctccagga
    12241 tatacagatg aagcccttga aatattaaaa ggaaagcgta aaggtaacta taatattgta
    12301 aaaatagatg aaaattatac tcctgaacct attgaaacaa aggatgttta tggaattact
    12361 tttgaacaag gtagaaatga aatattaata aatgaagacc tattaaaaga tattccaact
    12421 gataataaaa tttttacaga tagtgcaaaa cgtgatttaa ttatagctct tattacatta
    12481 aaatatacac aatcaaattc tgtttgttat gctaaagatg gacaagtaat aggggtaggt
    12541 gcaggtcaac aatcacgtat tcattgtact agacttgcag gtaacaaagc tgatacttgg
    12601 tatcttcgtc aacatcctaa agtattaaac ttaaaattta aaaaggatat aggtcgtcca
    12661 gatagagata acactattga tgtatatctt tctgatgatt atatggatgt gcttgctgat
    12721 ggcatatggc aaaacttttt tgaagaaaaa ccagagcctc taactggaga aggaaaaaga
    12781 gcatggctta aaactttaac aggagtagca cttggttcag atgcattttt cccatttgga
    12841 gataatatag aacgtgcaaa aagaagtggg gtaagtttta tagcacaacc aggtggttct
    12901 attcgtgatg ataatgttat attaacttgt aataagtata acatagtcat ggcatttact
    12961 aaaaatagat tattccatca ttaaaatact gaaagtataa agataatata gattaagtaa
    13021 aaatctacaa caagttttgt tgtagatttt tttatttgct gtataatttt aaatagaact
    13081 ttatttttag atattcaatt tggaggttga tatggcttta aatataagaa actatagcct
    13141 attatgtaat caaggaacag ctaaatttaa tgaagatgta gtcggtataa atccatttgg
    13201 ggcatgggtt ttagatggtg ctacaggact taatggaaaa aatttagtgt caactgaaag
    13261 tgacgcaagg tggtatgttc agtggtggaa taggtattta tataaaaata taaataaaga
    13321 agaatcatta aagagtatta ttaatactgg aataatagaa gtaaaaaaag agtatgaact
    13381 aagattaaat gggtttaagg ttgaaaaatt agatttacca tctagttcta tagttgttgt
    13441 aaaatttcat gaaaataaag tagagtattt tttattagga gattgtgctt tgtttattaa
    13501 aaatggagat tccaaagtta taaaagatag gagtatttgt aaatttgata atactgtatt
    13561 tgatgaaatg agtaaacttt ctaacttgga agagatggct tttgatgaga ttagaactag
    13621 tgttatggat acaatagtaa gcaatagatt gaagaaaaat acaaaagagg gctattggat
    13681 attggatttt gatgaggaag ctgtggaaaa ttctatatat ggatacattg aaataaaaaa
    13741 agattttcaa ttgatgctaa cgagtgatgg ttttacaagt gcatgtgata gatataatct
    13801 aataaaagaa gaagaattga tacgtatagc agaaaaattt ggtataggat atatacataa
    13861 taaagtaagg gattttgaag ataatgacta taaagcggtt aaagtgccaa gatttaaagt
    13921 aaaagatgat tcatcttgtt tatatttaga tatatataat gattagactg tataaaacta
    13981 tttaatttta gaaaggaggc aagtgaaaag tgaaaattct tcatataata acacaaaaac
    14041 caaatagtac aggtagtggt atatatttgt ctggtatgat aaaagggttc gaaaaaatcg
    14101 gtcataagca agctgtaatt gcaggtatag atgtaaatga tgatgtaaat tgttttccaa
    14161 gtgaagtaag tttttatcca gtaaagtata attgtgggga gttaaatttt cctgttgtag
    14221 gtatgagtga ttctatgcca tatgaaagta caagatataa agatttaaat atagacatga
    14281 taaatagatt gaaatatcaa tttaaagtaa atatagataa ggctatgaat gattttaaac
    14341 cagaacttat aatatgtcat catttgtacc tcttaactgc atttgttagg gaaatggtaa
    14401 aagatataaa ggttatgtct atttgtcatg gaacttgttt aagacagtta aataccattg
    14461 atttagagaa agaatacata atagcaaata taaggaaatt agatttaata tttgctcttc
    14521 atgaaaatca gaaatatgat attataaaga cttttggagt aagtgaaagt aaagtagttg
    14581 taattggtag tggttataat gatgatatat tttataataa aaattataaa ataaaggatg
    14641 ataaaataaa aatagtattt gcaggtaaga tatgtaaatc aaagggactg attccattta
    14701 taaaagctat atctaaatta aaatatagta aagatttaat agaagtcaat tttgcaggta
    14761 ctggaagcga tatcgaatca tataatgaga tagttaaact ggcaagtaag tctccattta
    14821 aaatgaattt cctaggaaag ttagaacaga gagatttagc tgagctattc aacaggtcac
    14881 aaatatttgt gttaccatca ttttatgagg gattaccagt tgtagtttta gaagctttat
    14941 cttgtggcac agatgtaatc actactgata ttcttggtgt taaagagtgg attggtagtg
    15001 agattaataa ttctggaaag atagaatatg tttctctacc ttttatggag aaagaaggta
    15061 ttccaaaaga tgaagagtta tatgattttg aaaataattt gtataatgca atagattcta
    15121 agatacaatc tttattgaat aactcaaata agaaaacatc tgtagatatg tcaaaaaaaa
    15181 cgtgggatgg cttagcatat agaataaatg aagttatttt aatggatgaa ttgtgtttag
    15241 tctaaaatta tttaaattat aaaaaagtgt aactattttt acactatttt taaatgattt
    15301 tatattatac taatactaaa tatatttgtt ttagggaggt aatttatgag gattagtaaa
    15361 aaggtattgg cgttaggtat ttcagcatta ctgttaagtg ttagttttcc aacatcaata
    15421 aacgcactag ataagattga aaatattcaa ggagtagata aatatgaaac agctgggctt
    15481 atttcagata agcaagattt tacaacagca gttcttatca atgcagactc tacaatggta
    15541 gatgggattg ctgcaagtgg tcttgcagga gtaaataatg cagctatttt attaactaat
    15601 aaggatgata taccagaagc aacacttcaa agactgaata gagttactaa gatatatgta
    15661 ataggtggag aaaattctat aagtaaagat gtagaaaaaa tgttacttat gagaagaatg
    15721 caagttataa gaattgatgg tgtagataga gtagatacaa gttataaaat tgcaggtgaa
    15781 atagaaaaaa ttaaaaacag tgataagatg tttttagtaa atggatttaa ggatgaagct
    15841 gatgcagtaa gtgttgcatc tgttgcttat agagatggag ctcctataat attgacaaaa
    15901 gatatacctt cagcagaaga agacacagac ttagacccat ggtttggagt ttctcctgtt
    15961 ccagtatatg ctattggagg agagtctaca ttaagtgatt acatagtgag cagatataga
    16021 gcaactagaa ttggtggtgt tgatagatat caaactaata aaaatgttat tgaaaagttc
    16081 tataatggag caaaagagtt ctatataaca agtggagatg atttagttta tgctttagtt
    16141 gcatcaccac ttgcaaaaaa tgctcctgtt gttttagttt caaataaaag tgataagagt
    16201 atattaagtg gagctagtaa agtaacagca ataggtataa gtgataagag tattatagag
    16261 caatgtttgg atgctgtaaa gaagtaatgg agtgttatta gtaaaatgaa tatatacatt
    16321 gaaaattagt agattgttca ttttataatg gtataaaata tatgggaaga ggctatcaaa
    16381 tggagataag cctctttttc attgttaaaa ttatgaaaag atactttatt ttatataaaa
    16441 aagtatctta tgaactaaat gtctattttg agacactatc tacttttctt atattatatt
    16501 attttttata tggaataaat ttcataagta ttatcttcct ttaatttttc taaccaaacc
    16561 atctctaaaa tatctagttc ttctaaatag tctttctctg ttttgttatc atcatgtttt
    16621 ataagttcta aaacttcaaa tgtaaatgct ttttcaccat attttttcca ttcattattt
    16681 aattctttta tcatacatga gccagcacca agctgaaatt tgagagagtt aaatttgctt
    16741 tttatgtcat tactttttcc aaggtaaatc tttttagtaa tatcattttt tattatgaat
    16801 acacctgtgt caaatttcat ttctttgtat aattgtttta attcacgttt tctatccatg
    16861 attttagata cctctcttta taattgatat gtaatatgta agtgtaaaaa tatattacat
    16921 atcaattata aagagattaa ttttatatgt aaatagtttt tataaattta attatgaatt
    16981 agtcatcaat aaacaagaaa tttagctgta aaaatccatt ataaaggcta ttataaattt
    17041 taatatgcta aaaatataaa aagaatagtt gacagtgtag aaaatatgga ataaactaaa
    17101 agtgtaatag taatatgaag ttatatatta cattgaaagg tgtgaatgat tattatgaat
    17161 aaaaaaatag aattaatagg gaattttgca agtgattgga ttaggtatag tgattatgaa
    17221 tataaaatta ctgaagaaga tgaattatat atagttccaa cagaagatgc tgtgtttacc
    17281 atgtataacc catttgatgt agcagatgat ataatagtag atataattcg cataggacaa
    17341 gaggcattac aagatatatc aaaagagaca aaagaaatga aactaaagaa agaagtactt
    17401 gaatttgtaa aaaagtatgg attattgggt ttaatatatt caagtgtata taatagagat
    17461 atagttggag aagaaagagt actgatgatt gaaaagaatt atattacaaa agagaatata
    17521 ttgagtacag atgaatatgt aaacatgttt ataccatttg caagtgatga agatgtgtat
    17581 tttaaaaagt atagaagggg tatagatgta tcaaagagag aagaatctcc aaagtttttt
    17641 ggaaagagac cattgatttt agatttagta ttttcaaagt tctacactga acaactagat
    17701 tggattgtaa gttttgctaa agatatgtgt aagcatttta atcaaatttg tgtatataaa
    17761 aatacatcta atctattaac tgaaagtgtt actattatgt ctggagggtt taatcctcaa
    17821 aagataggat tttctataag ccaattagat aaaccaatta ttgcatggga gtttgattct
    17881 ttgaaaacag ctattgagac tatctatgcc tttgcaatta caagtgaacc ttcttcttta
    17941 aataaatgca agtactgtgg taaaatttat attcctacaa attctaaatc tcaatactgt
    18001 tcaccttctt gtagaaattg ctcaaatgta aagaaaagta gaagtagagc caaaaaagat
    18061 aaataaaatt aataaatata tcattgttta gattttagta aataactact gaattaaaag
    18121 ttgttataga aaattatata tctaaatgat ggtatttttt tgttgagaaa attgaaaagt
    18181 ttaattgaga tagtaatttt gagttgacaa aaaccacttt taacactact attataagtg
    18241 gttggtaaaa taatatacat taatttaagt atagcataaa aaacaaaaaa agtcaatgga
    18301 aaaaataaaa aaggagaatt taaaaatgat atcacctatt attttgagtt ctataaatca
    18361 gaatttaaag gaaatagaaa gaaatgaact gcttgaaact aatatagaat ctggagatta
    18421 tggtctagct ctttctgaat ctgatgtaaa agatataata aattcgagag ataatacttt
    18481 aaaggggtat ggaagaatag aattagatat aaaagttaca aaacagttaa tagaaaatat
    18541 atatacatca caatatacaa atgtggacaa ttatttagag gctattaatg atatgcaaga
    18601 aattttttat tatttaaaaa atgaaactga tgacaagata tgtgatgatg agattataga
    18661 aatactgggt gaattttatg aaaaattttc aggcaatatg gacaatgtaa gaggagaagc
    18721 agacgaattt gcaaagaaat ttaaatttgg agaagtttaa tatatgatag gagagatttt
    18781 gtatgttaaa taatttaagt aatattaata taaacgaaaa taatttaata aagaaccaat
    18841 attccatatc tctacttaag gaatgtctaa attgtaaagt gatagatgaa agagaagtat
    18901 ataatataca acaagaagta tctctaatac ttatggactt gattaaaaaa tatacaaatg
    18961 gtcaaagcac atcagtaaaa acagaagttg ccgaaaaact gttgatttca atatggtatg
    19021 ctattgatgc atatataaat aagcttggag atatagaaaa aagagttgtg gagttgactg
    19081 ctaagaatgt taaaaaagtg taccttggag gcattgaaat attagaggaa gagttagtta
    19141 ggctaaaaga tttttataaa ctaatgatga gtagaaaatt ggatactaat tcaatagcat
    19201 ataatgatac cttgatggag ttatcaagtt tctttaaaag ttatattgta aaatttgaag
    19261 ctcaggacgt tccagtaagt atagattatc cattggcact agatgatatg gatgttaagg
    19321 gtatttatta tgtgaagaat tatattgaga gtatagatat agaaaataga ttttgtaacc
    19381 tatttaagaa aaaagataga gaaaaattat tttatgacta tgggatgact tataaaatag
    19441 attgtgatgt tatacttgta aatttatttg aacttattat gaataattgt attttttcta
    19501 ctattttagg aaatgaagtg actaatttag aaatttcaaa atatgagtat gaatttttag
    19561 aaaaacaatt taaaaatatg tacaataata ttactgaaga taaagttata gactctgaag
    19621 aaagtaatat aattgaattg attgaagtag atgatagttt taacactgaa gtagatagta
    19681 atttggatta taatgatttt gaagaaatta acagagaaat tagaataaat aaaaaaataa
    19741 ctttactact tatggaagca attgaagtat taataaaaag attaagcata gaagatgaag
    19801 atgtaataag atatattaaa aaatatgaaa agatatttat tgaatctgtg ctaagctcaa
    19861 taaaaagtaa cacttttaaa agtatggtaa ctgtgagtaa aaataaaaag gaacacttag
    19921 aagattatat tgttgatgat gaaagtaaat tggatgatga atcgtttaga gagatattca
    19981 atgagatatt agatagtaca tctataattg aaaaaataag aataataaag gaaaatatca
    20041 atgctaaaaa agactttatc gatattcttg aatctgaatg tttatttgga gaagagtatt
    20101 taatgttgtt tgcatcatta agtgaattag aattggcaat attgggtagt gtagtatttt
    20161 atgaagatat tagaatgaga gaaattaata tattagaatt tatgttaaat gagaaaactg
    20221 aaactacttt atggaagata gagtacatta aatttatgaa gaggcaaagt gaagataaga
    20281 tatactccat agaagagcat atgaataaaa ttaattaaca aaaaattgtg aataaataga
    20341 atgttagtat ataatataat ctgtaaagca tatcaaaaat acatatattt ggaggaaaat
    20401 tatgaaagag tatatcataa ataatgttga agaaataaga gaagaattaa ttgatttatc
    20461 taagaaaata tgggaaaatc cagaattagc ctttgaagaa aagtatgcaa gtagcattca
    20521 aaaagaatac ctaaagtcaa aaggatttaa aattgaagaa gtagaaaatt tacctactgg
    20581 atttatagca agttttggag agggtaaacc tgtgattggc atacttggag aatatgatgc
    20641 tttaccagag ctatcacaat gtgtaagtgc agaaagaaag ccattggtag aaggaaaagc
    20701 tggacatgga tgtgggcaca acctgttggg tgtagcaggt gtaggagcag ttgtttctat
    20761 aaaaaaatta atagaagaaa ataaatttaa tggaacaata aaatattttg gttgtcctgc
    20821 tgaagaagaa ggtggaggaa agacagttat gtgtataaat ggctgttttg atgatgtaga
    20881 ttgtgcattt acatggcatc cttttgatat aaatgcacct tggagaggtg gaagtctggc
    20941 aaacttatct gtaaaattta aatttaaagg gataacagcc catgcagctc aagctcctca
    21001 taatggtaga agcgcacttg atgctgttga aattatgaat gttggagcta attatttgag
    21061 agaacatgtt attgactcta taagaatgca ctatgttata actaatggtg gtggaagacc
    21121 taatgtagtt cctggatttg ctgaaagttg gtactttatt agaggtaaaa aagcaaagga
    21181 tgcagaacac gttcttgata gattaataaa agtagctcaa ggagcagcaa tgatgacaga
    21241 aactgaaatg gagtataagg ttacagatgg aatatatgac tatataccaa accaatgttt
    21301 gactgattta gtttatgaca atatggtttt tgttggttgc cctaaaaata ctcctgaaga
    21361 agaagaattt gcaaaaaagt tgtgtgatac tcttactaga gaagaaagat taggtgtagc
    21421 aacatcatta caaaatgata aatctattgt agaaagctat atacacaagg aaatagttga
    21481 cggtgacaaa gataaaggac tagctggttc tactgatgtt ggagatgtaa gttatgtaat
    21541 accagtggct caatttgcaa tggcagcttg gcctgttgga attgcaagtc atacatggca
    21601 gtcatgttca tcagcaggtt caaatatagg attttctgct atgataaatt cagctaaagt
    21661 actggcttgt agtgcttatg atgtatttat ggatactaaa attattgatg aagcaaaaat
    21721 tgaatttgat aaatcattag atggacaaaa atttaaacca ttggtataat tttgctaata
    21781 aaatatttct ggggggaata gctatgagag aaaaagttga aaaagtgctt gaggaaaaga
    21841 taaaaccagt attacaaaga gatggtggag atgtagagct tatagatgta aatgaaaatg
    21901 gtgtggtttt agttagacta caaggggcat gtagtgggtg tccaggagtg actatgacaa
    21961 tcaaagctat tattgaaaat gttttggtga gtgaagttcc aggagtaact caagtattag
    22021 gtgtttaata gttagcgttt attaaatgta tgttatacat aagtatttaa taaagaagta
    22081 aaaaaaatag tgaagcaata tatatgaaaa agtatgagaa agataataaa acattataga
    22141 aataatgaaa tatttcatat gtatatgaag aaataagaaa aaatagtgaa gcaatatata
    22201 tatgaaaaag tatgagaaag ataataaaac atttatagaa at
//
LOCUS       DAMJYX010000057        22194 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJYX010000057 DAMJYX010000000
VERSION     DAMJYX010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22194
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.057"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            155..496
                     /locus_tag="PC479_002263"
     CDS             155..496
                     /locus_tag="PC479_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2804804.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            647..1060
                     /locus_tag="PC479_002264"
     CDS             647..1060
                     /locus_tag="PC479_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2804805.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1262..1903
                     /locus_tag="PC479_002265"
     CDS             1262..1903
                     /locus_tag="PC479_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2804806.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1927..3180
                     /locus_tag="PC479_002266"
     CDS             1927..3180
                     /locus_tag="PC479_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2804807.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3226..3876
                     /locus_tag="PC479_002267"
     CDS             3226..3876
                     /locus_tag="PC479_002267"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2804808.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3927..4523
                     /locus_tag="PC479_002268"
     CDS             3927..4523
                     /locus_tag="PC479_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2804809.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4536..5375
                     /locus_tag="PC479_002269"
     CDS             4536..5375
                     /locus_tag="PC479_002269"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2804810.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5620..6774
                     /locus_tag="PC479_002270"
     CDS             5620..6774
                     /locus_tag="PC479_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2804811.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7080..8777
                     /gene="ade"
                     /locus_tag="PC479_002271"
     CDS             7080..8777
                     /gene="ade"
                     /locus_tag="PC479_002271"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2804812.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            9129..9593
                     /gene="bcp"
                     /locus_tag="PC479_002272"
     CDS             9129..9593
                     /gene="bcp"
                     /locus_tag="PC479_002272"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2804813.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            9603..10346
                     /gene="yaaA"
                     /locus_tag="PC479_002273"
     CDS             9603..10346
                     /gene="yaaA"
                     /locus_tag="PC479_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2804814.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            10474..13131
                     /locus_tag="PC479_002274"
     CDS             10474..13131
                     /locus_tag="PC479_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2804815.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      13185..13287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13397..14662
                     /locus_tag="PC479_002275"
     CDS             13397..14662
                     /locus_tag="PC479_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2804816.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14692..15582
                     /gene="metA"
                     /locus_tag="PC479_002276"
     CDS             14692..15582
                     /gene="metA"
                     /locus_tag="PC479_002276"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2804817.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            16073..16516
                     /locus_tag="PC479_002277"
     CDS             16073..16516
                     /locus_tag="PC479_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2804818.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            16547..18364
                     /gene="ftsH"
                     /locus_tag="PC479_002278"
     CDS             16547..18364
                     /gene="ftsH"
                     /locus_tag="PC479_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2804819.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            18542..21244
                     /locus_tag="PC479_002279"
     CDS             18542..21244
                     /locus_tag="PC479_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2804820.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            21278..21976
                     /locus_tag="PC479_002280"
     CDS             21278..21976
                     /locus_tag="PC479_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804821.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
BASE COUNT         8877 a         2369 c         3985 g         6963 t
ORIGIN      
        1 tttaaaaatt aatgttaaaa aataaactta tacattaata ttaataataa ttatttaaat
       61 atcttaagta aatcagtctt acatatattt ccgatagatt atattgataa aaaacactaa
      121 aattaaatta ataaaagtac ttggagggat ggaaatgaaa gaggaaaatt ttattaatgt
      181 tgaatacgag gataaacgtt tttcaattaa tagtaaagaa gttttgtaca tagaaaagca
      241 agagtatgaa gtaaagattt atactgaaag tggtaattat gaagttggag aaaatgctcc
      301 ttgtataaga aaactattga aagaagaaaa ttttttcaaa tgcagtaata gttatattgt
      361 aaatctagac aagtgtgaat tttatctcag caataattgt atttttgtga ataataattt
      421 tataagaatt ttaggttcag aaagaaaaaa gaaattaaaa aagaaattag aaaaattata
      481 tgattatgaa gattaatatt gggaggctgt attttataca gcctctttat ttattgattg
      541 tattattaat aaattttaaa ttatgataaa atataaaaca agtaacttat ttcagaataa
      601 aatacttttt aaaagaaagg gacgagatat gggagattat aataaaatgc ttgaattaaa
      661 gaataactta ggtatcagtg aaagaaagtt aaaatatcct tgtatctata aacatttcaa
      721 agggaaatat tatgctacaa tgggtcttag caaggctatt gatgatatag aaaacatatg
      781 tgaaatatat ggtaaagaaa atttaatcca aaacagaaat aaatataaat tagttattag
      841 acatactgaa agagaagaag atatctatgt ttatagggat ttagatggaa acttttatca
      901 taaaaaagaa gaagatacta atgatttagt tttatacaag accttatatg atgatacagg
      961 aatatttgca agacctttgg atatgttttt agaaaaagta gatacagata agtatgtcaa
     1021 ttcaattcaa gaatacagat ttgaagaagt atataaataa tataagagaa atatgtttat
     1081 aaatttagaa aaattcttta acagtacaat aatttgcaga aatacaaatg agatttattg
     1141 taaataatat gttagattag attataaaat agaaacaaag taaagtatta tatgatataa
     1201 ttaagttttg agaagtgata atttatattt aataaaaaat aggcatatat taggaggaca
     1261 tatgggaaat actttttata tagtaagaca tggacaaacg gattggaata tattgggaaa
     1321 aacacaaggt catggtaatt ctgaccttac gcctcaaggt atagaacagg caaaagaact
     1381 ttctgaagat attggcaagt attcaattga ttatattttt agtagcgacc ttgggagagc
     1441 aatgcaaaca gctcaaattt taggagataa gctcaatatt gaagttcaaa aaactgaggc
     1501 acttagagaa atgggattcg gagtttggga aggcctttta ataaaagaaa ttcaaaaaga
     1561 ttattcagat atatatgcaa catggagaaa tgaaccacac ttagtaaata taccagaagg
     1621 cgaaactctt aagattataa aggaaagagt agatgctttt ataaaggaac taaatgaaaa
     1681 atatgataat aaaaatataa ttcttgttac acattcaatt acattgaggg ttatgttgtt
     1741 gtcattttta gaatcaggta tggaaaatat atatagaatt aagcaagata atactgcttt
     1801 aaatatagta gaatttaaag attatgggcc tgttatagta aaaatgaatg atacaagtca
     1861 tataaaaaat catgtaaaga taaataattc tgcactggag taggaatcaa ttataaggag
     1921 aaatttatga gtaaagttat agtagttggt ggaggagctt ctggaatgat ggcagctttg
     1981 tcagcttcaa aaaacaataa tgaagtaata ttagttgaga gaaatggaga attaggaaga
     2041 aaacttaggg caactggtgg cggtagatgc aattttacca ataatagaga gatagaagat
     2101 ttttttgata aagttgtcag taataaaaaa tttctctata gtagttttta tacatttaca
     2161 aataaagacc ttatttcgta ttttgaaagt agaaatttgg aatataagat tgaagaagaa
     2221 aatgaccaca aagtatacac taaaaatgat aaatctatag aagtaataga agtattaaat
     2281 aaagatttac tcaatcacaa tgtaaagata atgtataata agaaagttat agatattata
     2341 actgaagaaa ttgctctaaa agatgattct aataaggaca agtcaaagta tctaataaaa
     2401 gggattattt tagataatgg agataaaata ctaggagaca aggttattat atcaactgga
     2461 ggagtgagtt attcaaagac gggttcagat ggttcaatgt ataagattct taaaaagcat
     2521 ggacatacat taaacaaact atatcctgct ttagttccac ttacaataga ggaaaaatgg
     2581 attaaagatt tacaaggaat atccatgaag aatgtggaaa tatcttgtaa gattaagaaa
     2641 aggaaaatat caaaatctgg agacatgcta tttgcccatt ttgggataac aggaccttgt
     2701 gttcttataa tgtcttctta tattaacaag attattgaaa aggaaaaggt agaattaaat
     2761 atagatttct taccaaattt aagtactgat gaaatctcta gtataatcag agcatttcca
     2821 aataaaaatg ttctaaataa tcttaagcaa atactacctc aaaatttctt aaaagagatt
     2881 ttttctctat tatctttagt tgataaaaag gcaagtgatt tgtctaaggc agatgaaatt
     2941 agaattattg agtatataaa aaatatgaag ctaacttgta atgggactac aggtataaac
     3001 actggtatgg ttacttctgg tggtatttca gttaaagaaa ttaattcttc tactatggaa
     3061 tctaaattag ttaagaactt attttttaca ggtgaagtaa ttgatataga tgcagaaact
     3121 ggtggatata atttacaaat agcattttca actggatatc ttgcgggtat aagtgtataa
     3181 atataatttt ttaataatta aatatgaata aatagaggtg tatatatggg taatttagtt
     3241 atagctgtag atggtccagc aggagctgga aaaagtacaa tagccaagat agtagcaaaa
     3301 aagctaaata taaattatat agatacaggt gctatgtaca gagcagttac ttataagtgt
     3361 ttaaaaagtg gtattgatgt caataatgaa aaagaagtta ttcaaatagc tgaaaattcg
     3421 gatatagatt ttaaagataa caatatatat ttggacaaag aagttataaa tgaagaaata
     3481 agaacaatag aagtaagcaa taatgtttct aatgtagcaa aaataaaaga agtaagacag
     3541 ttgatggttg aagttcaacg aaaaatagga atgaaaaatt cagtaatact agatggtaga
     3601 gatataggtt catatgtttt cccagatgca gattacaaat tctttttagt agctactcca
     3661 gaagaaagag gaaatagacg ctataaagaa ctttgtaata aaggatataa tactacttta
     3721 gaagaagtaa ttgaggatat aataagacga gatgaaattg attcaaatag agaatttgca
     3781 ccattggtaa aagctaatga tgcactagaa atagatacta caggaaaaac tatagaagaa
     3841 gttgtagaag aagttgtatc taaaataaat ctttagtttt ttatgcatca taatgaaaat
     3901 aaatgtagtg ttgaagggag ataaacgtga atttttatag atttgtaata aatattttta
     3961 agggtttttc taaaatattt tttaaatatg aagttatagg tgcagagaat attccagata
     4021 gaggaaatat agtaatagca tcaaatcata aatcgaatct tgaccctata ttcttggctg
     4081 ctgccataga aaatagagaa atagctgcaa ttgcaaagaa agaattgttt aaagtaaaac
     4141 cattgggatt tatattaaaa aaattgcatg ttatgcctat aaatagagaa aaaccagatg
     4201 tatctacaat aaaaactatt ttaaggtcag ttagagacgg ttatgtatta ggaatatttc
     4261 cagagggtac tagaataaaa ggagattctt ttggtaaggc taaggcaggt ctttctgtat
     4321 ttacaataaa aagtaaatct aaagttgttc cagtatcaat aatatctaaa tataaacttt
     4381 ttagtaaagt aattgtatat attggagaac caatatcatt tgaagaacat tttaaggaaa
     4441 aattgagtaa tgatgaccat gaaaggatat cacaagagat tttagaagtg ataaaacaaa
     4501 attactttaa atattcaaaa taagggggta aagttatgaa cataaaaata gcaaaaaatg
     4561 ctggcttttg ttttggagta aaaagagcaa tgaaaatggc atgggatgaa gttgaaaaaa
     4621 atgattctgg catatatgct ttaggtcctc ttattcataa caagcaagca gtagcaaaat
     4681 atgaagaaaa aggtcttaag actgtgaatg aaattgatac aataccaaat catgaaaata
     4741 tgataataag gtctcatgga gttcctgaaa atatatataa ggaagccaaa gataaaaaat
     4801 taaaaattgt ggatactaca tgtccatttg taaagaaaat acatacagta gtaagtgaat
     4861 atcataataa aggctatgaa ataatagtta ttggagatat gaagcatcca gaagttatag
     4921 gtataaatgg atggtgtgaa aattcagcta taataataaa gaccttagaa caaatggaaa
     4981 atatggaatt tgataactcc aaaagatatt gtcttgttgc acagactaca ataaatccag
     5041 agttgtatat aagtatagtt aataaactat cagataaatt agaagaaata gtatttaatg
     5101 atacaatttg ttcagctaca aaaacaagac aagaatcagc taaagagtta gctaaagaag
     5161 ttgattgtat gatagttata ggaggtaaac acagttcaaa tactcaaaag ttagtaaagg
     5221 tttgtgaaga cttagtacca acttttgcaa tagagactaa agatgaattg gatgtaaata
     5281 cgcttaaaaa atataaaaat ttgggtatta cagcaggggc atctacacct aattggataa
     5341 tagaagaagt tgtaacattc cttgaaaatt tataatttga taggtaataa aaataggtgg
     5401 acattgttat aatttatcat aatatccacc tttgatttca tattacatgt ggtaaataga
     5461 taacctaata tcaaaaagtg tgtatcttaa gatgaaaaac taagagtata agatgcgcac
     5521 tttttaatat tagaaattca aaagaaatat tgatattgat tatcaataga aaatagaata
     5581 taatataatt gttgcaaaat tagtctagga ggtaaattaa tggaaatgtt gatatccgct
     5641 tctgaagagg cgtttttaca tgtaggttct atgattggat tttttatttt gttatttgga
     5701 tatattaatt ataaaactag tggaaatttt actaatatca tatcaaaaaa cagaaaattt
     5761 caacccttaa taggtgcttt aattggagct ataccaggtt gtggcggaag tttggcaata
     5821 atgccattgt atataaatgg aaaattaagc tttggagcaa taattgcaag tttaatagct
     5881 agtatgggag atgcagcatt tgtacttatt tcatcaaata ttaaaatgta tttttttgtc
     5941 actatagtta gtacaattac aggaataata actggacaat tagtggatta ctttaaacta
     6001 gaagaaaaat tagggttaaa aaaccgtaga aaatcagata aatatgatat aagtaaaaat
     6061 aaccctaaag aaaataaaga acaacataat catgatgaaa taattttaga cactctagct
     6121 aaatctcatg gaaatacaaa tagacttgca tttataataa cacatggaaa gggatataaa
     6181 atttatatag gaattatagt aatcggtttt atatttatgt cacttgctca ctctggactt
     6241 aatcttccta taattgagaa gttacattca ttggaagaag taatagcagt gattggaata
     6301 ttattttcaa taatttatat gtggtgtttt aaaaaggtat ttaaaaattc taatcaaaaa
     6361 gaggaagaaa ataaaaagat ttctttgcga gaaatgctta tccattctgt tggagaaata
     6421 tcatttgtaa ttacatggat ttttattgca tatcttattt acgatttgat aatattaatt
     6481 ttaggtggag atgaatactt agttaaatta gtattatcaa caggtgtaat aagtgtattt
     6541 ataggtgcag gtcttggact tattccaggt tgtgggatac aaattgtgct tatgtcattt
     6601 tatttgaagg gaactatacc acttggtgca gtgatagcca actctatttc tcaagatgga
     6661 gatgcacttt tcccactttt agcaatggat aaaaaatctt ctttatgggc tatgattatt
     6721 actactatac cagcaatact tgtaggtatg atagtttatg catttttagg ttaaatataa
     6781 ttatgaataa agaagtatta aaaaaataat aaagcacata aatttattta taagaatagt
     6841 gaagattaaa tataaaaata aaaaattcaa tatgaattaa attattgctt aaatattaat
     6901 gagaacatgg gagataatat tattcctcat gttctctcta tttaattaaa ggaattatgg
     6961 aacaaatttc aagtgatact ttctatcaaa actcatatat aatatatatt aataaattaa
     7021 actataatac gtaatacagt cagtatattg atataaaaaa cttaggagga gttgtaaaaa
     7081 tgggtaattt aaaaagacag tgtgatgttt caagtggaag agaaaaagct gacattgtac
     7141 taaaaaatgg gacaatcata aatgtattta cagaagagtt aataacagga gatgtagcaa
     7201 tagtaggaga cactattgtt ggaattggcg attacaaggg caatgtagaa attgattgta
     7261 gtaacaaata tatatcacct ggctttattg atgcacatat gcatattgaa tctacaatgg
     7321 taatgccaat tgaattatcg aaaaaattat taaaatcagg tacgacaact atcatagccg
     7381 acccacatga gttggtcaat gtaaaaggag ttagtgcaat agatttttta ttagaatcga
     7441 caaaagatat accacttaat gtatatatta tggtgccttc atccgtacct gcaacaagtt
     7501 ttgaaactaa tggagttggt aaatttagtg caaaagacat ggagagttat gtaaataatc
     7561 ctagaatttt aggtttagga gaggtaatgt gttttaatga tgttataaat tctgaaaatg
     7621 aaatcttgga taaactagag ctttttaaaa ataaagttgt agatggacat gcacctaata
     7681 taaatggtaa atcacttcag acatatgtat gtgcaggaat agaaaatgac catgaatgta
     7741 taacttttga tgaagtatat gaaaaattga gagcaggatt aaaaatttta ataagagaag
     7801 gaagcgcagc aaaaaattta aagtctatag taagtggtat gctaaagcat aacttgccaa
     7861 ttgaagaatt tatgttttgc acagatgata agcacttaga tgatatagaa aaacaaggtc
     7921 acataagatg gaatataaaa tgtgcgattg atttaggtat ggaacctgtt agggctataa
     7981 aagttgcaac ttataattct gcaaaagcat atgggttaag gaaaattgga gcaataggtg
     8041 cgggttataa ggcagatatt gttgtattga atgatttaga caaaatggaa gtggatagtg
     8101 tatataaaga tggtaatttg gtaaacgaag agatgttttc taattacaat tatgagatta
     8161 aagataaaga attgcttaat acagtcaagt ttaaatatat taataaagaa aaaatacagt
     8221 taaaagtaag tgaaaaaaat tatgtaatgg agattgtacc ttatcaaatt ttgacaaata
     8281 aggtatatga atctttacca tgtgcagatg gatattttgt tccaaataaa gagtattcaa
     8341 aactttgtgt tgttgagaga catagaatga ctggaaatgt agcaattgct ccactaaaag
     8401 gatttggcat aaaaaatgga gcaattgcta ctactgtggc ccatgattct cataatatta
     8461 tagttgcagg ggataatgat gatgatattc tagtagctat aaattactta aaggaaatac
     8521 aaggtggtta tgtaattgta tcaaatggaa aagttcttgc acacctaagt cttcaagtag
     8581 ctggattaat tagtactttt acagctgaag aagtacaaga aattactgat aatatgttgg
     8641 agatagcaag aaaaatggaa gttccagagt atgtagaccc ttttattaca ctttctttta
     8701 tggcacttcc agttgttcca caaataagac ttacagattt aggtctattt gatgttgagg
     8761 aatttaaatt tatatagata gtataaaagt aataataaat gaggctttct caaaatagag
     8821 ataagtctct ttttattgtt gaaatgatga aaatatattt gatttcatac aaaaaagagc
     8881 atctcatgaa ctaaaaattt tatttttaga tatccttatt ttaattaatc tatcatttaa
     8941 cattgtttta agcatagata tcttaataaa actattttca gtctatttta acatataaat
     9001 acatgaaaat tatatattta tgaaacttta tacataaaaa ataaaatatt ttatttatat
     9061 atagatttat agggtaataa tataagcata gtataaaatt attaataaaa acaaaaggag
     9121 agatatttat gttgagtata ggaacaaaag caccagaatt tacactagaa gataaagatg
     9181 gaaataaggt aagtatgtca gattttaaag gcaaaaaagt agtagtatat ttttatccta
     9241 aagataatac accaggatgt acacgtcaag cttgtgcttt tagaaatgca tatgatggat
     9301 ttaaaaagga agatatacaa gttattggga taagtaaaga tagtataaaa tcacatcaaa
     9361 aatttgcaga aaagcatgaa cttcctttta ttttactttc tgaccctgac ttagttgcta
     9421 taaaagcttt tgatgtatgg aaagagaaaa aaatgtatgg aaaaactgca ttgggagttg
     9481 tacgtgcgac atatattata gatgaaaatg gaattattga aaaagtcttt gaaaaagcaa
     9541 aaccagacac aaatgcacaa gagattcttg aatatttgga aaaacaggag taataagaat
     9601 ttatgattat aatgttatca ccagctaaaa atatgaagaa tatagaagta tttgatagag
     9661 atttgagttt gccttgcttt atagataaca caaaagaaat agtggaaaat ataaagacat
     9721 ttggtataga agattttaaa aataaaatga aaattaatga aaaattagct gtattaaata
     9781 aaaatagatt tgaaagcatt aaatttgata gattaggaaa ccctgcaatc ttaacttatg
     9841 atggaataca atataaaaat atagaagcag aaaattttac aaggaaagat gaagagtttg
     9901 caaatagctg tattcgaatc ataagtggat tgtatggtgt tgtaaaacca tatgatagta
     9961 tatatgaata tagattagaa atgcagacaa aattacaagt aggtgagttt aagaatttat
    10021 acgaatattg gggaaatcgt atatataaag aactaatcaa agaaaaaaca gctatcgtta
    10081 atctttcatc aaatgaatac agcaaatcca tagaaaaatt tattaaagat agtgatacat
    10141 atattacatg tacttttaaa gtaaataaaa atggcatatt gaaagtggaa tctacacaag
    10201 caaagaaagc tagaggtatg atgacaaaat acattgtaaa aaataggata agagatattg
    10261 aagaactgaa aaagtttgat ttagaaggtt ataagtataa agaaaactta tctaacaata
    10321 gtgaatacat atttgtaaaa gagtagaagt tttgttaaat attaaatggc attgttaaat
    10381 ttaggtaaaa tatacttagt gtgtattgaa ttgaacttga ttttaaaata taattattct
    10441 taaattaaaa aaagaaagga gtggtcttcc aaaatggaaa atcacaaaca gatgccttgg
    10501 cattcaagag caacgcatga aattttagaa agtctagatt catcagagga ggggctcacc
    10561 gataccgagg cggaaaaacg gcttaaagag aatggcttaa atgaacttag aaaaaaacca
    10621 cctagaacaa ctttacaaat gctttggagt caaattacag atgctatggt tatgatacta
    10681 ataggagcag caatattatc tttgatattt ggcgaattta cagaagcttt tgtaatacta
    10741 ataattgtaa cagtaaatgc agttattgga attgttcaag agaaaaaagc agaatcatca
    10801 ttagaagcat taaaaaatat gaattcacct acagctcgtg ttatgcgtga tggtgaagaa
    10861 aatgttattc cagcaagtaa tcttgtagta ggagatattg tttttttaga agatggagct
    10921 atggttccag cagaccttcg tttaatagaa acatcaagtt taaaaattca agaggcttct
    10981 ttaacaggag aatctgttcc atcagaaaaa gatgcagata caatcttacc taaagaatgt
    11041 gtattaggag accgtagcaa tatggcgtat acatcttcaa tagtcacata tggtagaggt
    11101 gtaggtgttg tagtagcaac tggtatggat acagaagtgg gtaacatagc acatctttta
    11161 gattcacaag atgattttga tactcctata aaacgtaaac ttaatactgt tggaaagaca
    11221 ttaagtgtca taggtattat tgtatgcgtt gttatatttg caataggtgc attttatcag
    11281 agacctctct tgccaatgtt tatgattgca atttcccttg ccatatcaat tattccagag
    11341 ggattacctg caacagctac tattgtaatg gctttaggcg tacagcgtat ggctaaaaga
    11401 aatgctttaa ttagaaaatt acctgctgtt gaaacattag gaggagctac agtaatatgt
    11461 tgtgataaaa ctggtacatt aaccttaaat aaaatgactg ttacacatat tgctgtcaat
    11521 ggagattttg aatctggaaa agtgacatca atagatactg catctgaaaa acatccatta
    11581 gtgtataaag agattgttta tgctggtgca ctttgtaatg atgcatgtat taatccagat
    11641 aaaacagatg aaatattagg tgacccaaca gagggcgcat taatttttat ggccaaaaaa
    11701 tttggaatag atcaagaagc tcttgaagaa gaatatccac gtgtatttga acagcctttt
    11761 gattcagata gaaaatgtat gacaactgtt cataatatag atgaaaagat tattgcttat
    11821 actaaaggag cagtagacga aattcttgac ctatgtacta aaatattaac ttcaaaaggt
    11881 gaaagaaaca ttactgaagt ggataaaaag aatatacacg aattatgtct taacatgtct
    11941 aaagatgcat tgcgtgtact tggttttgct aagcgtgaga tttcatctat accaaaagaa
    12001 gatagtgaaa atatagaata tgatttaaca tttataggta tggttggtat gattgaccca
    12061 ccaagaactg aggtaattga tgcagtaagt acttgtaaag aagcaggtat aagaaccata
    12121 atgataacag gtgaccataa agttactgcc acaacaatcg ctcatgagtt aggaatatgg
    12181 agtgaagaaa atactgtgat atctggtgat gaacttgata atttgagtga tgatgaacta
    12241 gatgaagctg ttaaaaatac tacagtattt gctcgtgtat ccccatttga taaacttaga
    12301 attatacaat ctctaaaaag aattggagaa gtaccagcaa tgactgggga tggtgtaaat
    12361 gactctcctg ccttaaagtc tgctgatatt ggtatagcca tgggtataag tggtacagat
    12421 gttgcaaaag attcttctga catgatttta atggacgata gttttacaac aattgcttat
    12481 gcaataaaag aaggtagacg tgtatataga aacatacaaa aagttataca atttttgctt
    12541 gttggtaata tagcagagat acttacgttg tttgtagcaa tattattaaa ttgggatact
    12601 ccgcttcttg ctgttcatat tctatgggta aaccttgcaa ctgctacgct tccagccctt
    12661 gctcttggag ttgacccacc aagcagaaat ataatgaagc accagccagt gaaatcaacc
    12721 actttgtttg aaaaaaatct tatacaaaga gttgttaccc aaggtatatt tgtggcaata
    12781 atgactattt tagcatataa aattggtctt aatatagaaa atcattctgt aggtcaaaca
    12841 atggcatttt gtgtattagc attttcacag atgttacgag catttagtca gcgttcaaat
    12901 acagattcaa tgtttaatag aaataatggt aaaaatccat ttcttttaat ctcattttta
    12961 acttctgcat ttttaatagc agtaatacta tttgtacctg tttttagaaa tgcttttaac
    13021 ttaacagttt taaataactt agagtggctt gtaacgattg ttttagccat aatgtctgta
    13081 attcaagtag aggttgggaa aattattaaa agaaaaaaat taaaaaaata gttgacaaaa
    13141 ttaggaaaaa tatataagat ataaaacata gaaaaattaa ataaatctta tccagagagg
    13201 tggagggact ggccctatga aacccggcaa ccagtatatt tatatacatt ggtgctaaat
    13261 cctgcggcga aagctgagag atgagtataa ttaagcttat acagagcctg agttatatac
    13321 tcaggcttta tttattttaa ctctcatctg gtaaatagtt taattttttt tactaaaatc
    13381 taagggggat acaagaatgt ataataaaga aacaatatgt gtgcaaggaa attataaacc
    13441 aggtaatgga gaaccaagag tactaccttt atatcaaagt acaactttta aatatagcag
    13501 tatagaccaa cttgctgaat tatttgattt aaaagttgat ggacatatat attcaagaat
    13561 aagcaatcct actattcaag cttttgaaga aaaaataagt ttactagagg gtggagtatc
    13621 ttctgtagct gtatcatcag ggcaatctgc aaatatgttg gcagttttaa atatatgtaa
    13681 atcaggagat agtatacttt gttcttcaaa agtatatgga ggaacattca atttactagg
    13741 acctagtctt aaaaaatttg gtatagattt aatatcgttt gacttagatt caagtgaaga
    13801 tgagatagta gaacttgcaa aggaaaatac taaggttgtg tttgcagaaa cacttgcaaa
    13861 tccaactctt gaagtcatag attttgaaaa aatagcaaat gtagctaaga gaattaatgt
    13921 tccatttatt gttgataatt cattagcatc tccagtgctt tgtaaccctt taaagtatgg
    13981 agcaaatata gttactcatt ctaccacaaa atatttagat gggcatgctt caagtgttgg
    14041 aggaattata gtggatggtg gaaactttaa ctgggataat ggaaaatttc cagaattagt
    14101 tgagccagac ccaacatatc atggtataag ctatactcaa aaatttggaa atgccgcata
    14161 tgcaactaaa gcaagagttc agttgcttag agactatgga aattgtttaa gcccattcaa
    14221 tgcgtatctt actaatttaa atgttgaaac actacatctt agaatggaga gacatagtga
    14281 aaatgcactt aaaatagcta gatttttaga aaaacatgaa aatgtagatt ggattaatta
    14341 cccaggactt gaagataaca agtattatga gaatgccaaa aagtatttat caagaggatg
    14401 tagtggtgtt ttatcatttg gagtaagagg tgggttagaa aatgccaaaa aatttgtgga
    14461 aaaattacag atagcatctt tggttacaca tgtttcagat gtaagaactt gtgttataca
    14521 tccagcttca actactcata gacaattaac agaagaacaa ttaattgcat ctggagtatt
    14581 gccttcacta ataagattat ctgttggaat agaaaatgta gaggatttaa tagctgattt
    14641 aaatcaagct ttaaatttct aatattctaa gagaaagaga agtgaaaaga tatggcttta
    14701 atactaccaa agggattacc tgttattaat aaattattag atgaaggaat agatgtaatt
    14761 tataaagaag attttaaaaa ggaattaaag tatgaagaaa acatagacac taagatagct
    14821 atactaaatc taatgcctat aaagatagat acagaattag atttactaag aaggatagat
    14881 aaaacaggat tcaacgtgtc tgtggaattt attaaaattt caacaagaga aagtaaaaga
    14941 agttgtaatg aatatgttaa agaattttat aaaacatttg atgaagccaa aggagagtat
    15001 tttgatggct ttataataac aggagctcca gttgaacaaa tggagtttga agaagttgat
    15061 tattggaatg aactagaaga aattatggat tattctaaga gaaaaactaa atctacatta
    15121 tatatatgtt gggcagctca agcaagtctt tacaaatact acaatgtaaa aaaacttcca
    15181 ctaagtcaaa aatgttttgg agtatttaag cataaggtag ataaagatag taaaatagta
    15241 gatggatttg aaaatgaatt ttttgctcca cattcaagac atactactgt aaatattgaa
    15301 gctttgaaag agaataaaga gttgagcatt gtgagtcatt ctaaagaagc tggtccctat
    15361 ataattacaa attcaagaga tgtgttcgta atgggtcata gcgagtatga caaatacact
    15421 cttgataaag aatataagag ggatataaat aaaggagata aaatatctat acctcaaaat
    15481 tactatatta atgatgaccc ttcagaagag ccaactgtaa aatggaaaaa acattcagaa
    15541 ttgttgttta gaaattggat aaaaaactat ttgatacaat aaattttaag agtatataaa
    15601 tgttaactaa aaaaatatag aaatttatag gtataaagtt aatcttaatg atactttata
    15661 ccttattttt ttaaaatttg accttataat agtagtttta tattttaaga aaatcacttt
    15721 aggtgtatgt agatagaggt ggctttaatt ttatatatgg tgcgataatg ggagactaaa
    15781 cctttatagt atcaataaat ttgtatttta acttttaaaa gagattaaaa agggagaaat
    15841 cagtgtaaaa tatatatata gattttttat taaattttat taaaaatatc ctgtataaaa
    15901 ttaaatatca ataagataac aaaatttcaa ttgaagttac aatattgtaa ttgacaataa
    15961 atacttaata ctataatatt taatacatta aacattatag tgttaagtat ttatttacag
    16021 tatgcacttc agcctaaatt tgaaataaag tattttcgta gggagcgttt gtatggatga
    16081 gatgagtttt aagaaagagc tactagagtt gactagagat ataaatatga aatttacaac
    16141 tttattaagt gatttttatc agccacttgg tattacagca gtgcaagctt tgattttatc
    16201 tgagttatgt gaacatggag agaaaaaaat aagcgattta gggaagaact taaacatgac
    16261 aaatagtaat gtatcagtaa tctgtcaaag gcttgaaaaa aatggctttt taaatcgcat
    16321 aagagatata gaagaccaac gaattgtaaa agtaaaagtt acaaataaat cattggatat
    16381 acaagaacat atttcaagta gtatttttga tagttatttt gaaaatatga ccagtgaaaa
    16441 attgcaagat atggaagata taatagaagg tttagagaaa ttaaataaac ttttaactta
    16501 cattgattgc aaataacata aaaataattt aaaggagaat aaaattatgg atgaacataa
    16561 accccaaaaa aggtcagcac tctattatta tacagttata ataatagtag taatgttttt
    16621 aaatgcttta atatttccaa gtttaatgga taaaaaaatg gttgagattg actatggaaa
    16681 gtttctcaca atgttggata caggaaaggt taaatctgtt gagatagaaa ataatagaat
    16741 agcaataaat ccatctagta gcaaggataa gaatatatac ataactggta gaatggatga
    16801 cccagaattg gtagatagac taaaaaatgc aaaagtagaa tttactaagg ttattcctaa
    16861 ggaaaattca cctcttttaa gtattttact tacatggata atacctatag gtattcttat
    16921 gctatttggt aacttaatga tgaagtctat gcaaaagcga atgggtggta atgctatgca
    16981 atttggaaaa agcaacgcca aaatgtatgt atctgctcaa agtggaaaaa gatttaatga
    17041 tgtagctggt caagatgagg caaaagaagc tttaactgaa atagtagatt tcttacacaa
    17101 tccagaaaag tataaaaaga taggagcaca aatgcctaaa ggagcacttt tagtaggacc
    17161 tccaggtaca ggaaaaacac tccttgcaaa agctgtagca ggtgaagcaa atgtaccatt
    17221 tttctcaata tctggttctg agtttgttga gatgtttgta ggaatgggag cttctaggat
    17281 tcgtgattta tttaaacaag caaaagaaaa agctccatgt atagtgttta tagatgagat
    17341 tgatactatt ggtaaaaagc gtgataatgg aagtggatta ggtggaaatg atgaacgtga
    17401 acaaacttta aatcagttgt taagtgagat ggatggtttt gatggtggaa ttggagtagt
    17461 aatacttgct gctacaaata gacctgacag tcttgacaaa gcattacttc gtccaggaag
    17521 atttgataga cgtataccag ttgaacttcc agaccttgga ggacgtgaag ctatattaaa
    17581 ggtacatgct caaaatgtaa atgtaagctc agatatagat tataatgcaa ttgcaaggtc
    17641 tacatcagga gcatcaggtg cagaacttgc aaatattgta aacgaagcag cacttttagc
    17701 agttaagtct ggcagagata tagtagaaca acaggatttt gatgaatctg ttgaaactgt
    17761 aattgctggg tatcaacgta aaggagctgt aatttcagat aaggaaaaga aaattatagc
    17821 atatcatgaa ataggtcatg cacttgttgc tgctatgcaa aaacattctg cacctgtcca
    17881 taagattact ataataccta gaacttcagg agcattggga tatactatgc aagttgctga
    17941 agatgagagt gtacttatgt ctaaagaaga agcaattgat aaaataacta cttttacagg
    18001 tggtcgtgct gctgaagagg ttatctttaa cacttgtaca tcaggagcat caaatgatat
    18061 agaacaagct actaaaattg caagagctat ggttactcgc cttggtatga gtgaagagtt
    18121 tgatatggtc gctcttgaaa ctgtaagcaa tcaatattta ggaggagata cttctcttgc
    18181 ttgttcttcc gacactgctg ctaaaataga tgaagaggta tttaaaatag taaaggcttg
    18241 tcacaaaaat gcaattgaaa tcttagaaaa taatattgat aaattgcatg aacttgctaa
    18301 ttatttatta gaaaaagaaa ctattacagg tgatgagttt atggaaatac ttaacttaaa
    18361 ctaaaatgat tatacacaag ataaaaatgc ctattgttag gcatttttat tttacaacta
    18421 aattttttaa atttatattt aaatctttac ataatcttaa tcattaaaaa tacatcttaa
    18481 ctacaatttt atatcggaat tgatattata atagtaatga atggtgtaag gaggtttttt
    18541 aatggataga cctaatcctg atattctatt agaaaaaatt aaaaatgaag aagaaaaact
    18601 aagtagaggt caattaaaaa tattctttgg ttatgcagct ggagtaggaa aaacctactc
    18661 tatgttggaa tctgcacaaa atttaaaaaa agtgggtgta gatgtagtag ttggatatat
    18721 tgaaccacac actagaccag aaacattggc tttacttgat ggcttggaaa ttcttcctgt
    18781 aaaggagata gaatataaat ctataaaact aaaagaattt gatttagatg ctgctttagc
    18841 aagaaaacca gaagttatat tagtagatga atttgcccat tctaatgttg ctgggttaag
    18901 gcatacaaaa agatggcaag atatagaaga attgctgtta gcaggcataa atgtctacac
    18961 tactgtaaat gtacaacatt tagaaagtct aaatgacatt gttgagataa ttacaaacgt
    19021 atcagttaaa gagactatac cagataagtt tttggataca aatacacagt tagaacttat
    19081 agatgttgaa cctgatgtac ttcttgaaag gttcaatgaa ggtaagatat ataagaaaga
    19141 acaagctact agagcaaaaa ataatttttt tataaaagat aacctggttg cacttagaga
    19201 aattgcactt agaaaaacag cagagagagt taataaagaa gttcaaatga ccagactatc
    19261 aaagggtgat gtaactgtaa tacctacatc agatacactt cttgcatgta taagtccatc
    19321 tccatcttca gcaaaagtta ttagaactgc atccagaata tcagacagta catttgcaaa
    19381 atggatagct ttatatgttg aaactccaaa cacagcaaga cttccatttg atgaacaaaa
    19441 acaactacaa aataacctaa aattagctaa gaaattaggt ggagaaataa ttgttttaca
    19501 tggtgaaaat ataatagagc aaatacttag aattgcaaag ctgagaaatg taacaaaaat
    19561 tgtaataggt agaaatcatt caagtaataa aagatttagt aaaagattta aaaaagatat
    19621 agttgacaaa ttaattgatg aagttgatta tatagatata catataattc catataaatt
    19681 tgcctcagat gttaaataca aacctaaaaa ggataaatca tcaataaaat ctaaatttaa
    19741 aattagcaaa gttgatttta taaaacttat atttattact ttgatgataa gtattttagc
    19801 atatgcagta caaagcatgg gatttataag agaaaatata ttgttaatat atatgttggg
    19861 tgtagtttta gtttctatgt ggactaaagg gtatagtaca ggtataataa gttctgtatt
    19921 taatatagtt cttttaaatt atttttttac agcaccatta tatacattat ctatagcaga
    19981 ttcaaactat atagtaacac tggttgtatt ctcaatagta ggtataataa ctagcactct
    20041 tacgtctaag atacagcatg aagcagaaac tgctgctaaa agagaagaaa atacaaaaat
    20101 gatatatcaa ataattaagg gatttttaag attatcaaat aaagatgata tagtaaacaa
    20161 aggtatagaa cttttgtctc ttagcttaag tagagatgta atttgttatt taaatgtaga
    20221 taagcataat aaatcaaagc tttacaaaaa aaatactaat aataaggata aaaatgattt
    20281 ggatagtgaa gatgaaaaag ctgttgcaag ttgggtttta tccaattcaa cagtagcagg
    20341 aaatgacacg gatacacttc ctggttcaaa gggatactat ataccaattg taggaatgaa
    20401 tttaacttta ggagtagtgg gaatatcatg tattgattca aaattggata cagaagatat
    20461 aagcttaatt gaaacaataa ttgctcaaat ggcaatagcg cttgatagag aaatcttgtc
    20521 agaagctaaa gaaaatacca atcttgagat tgaaagggaa agattaagaa gtaatctttt
    20581 aagagcagtt tcgcatgacc ttagaactcc actagcagga atatctggtg cagtaagtac
    20641 aattataaaa aacaagggta ctataggtca agatataata gatgaattac ttagtggtgt
    20701 atatgaagat actcagtggt taataagact tgttgaaaat ttacttagta tgaccaagat
    20761 agatgaaggg aaattggaag ttaaaaaaca ttctgaactg gttgaggaaa ttatatcaga
    20821 agcgttacaa aaaataaaaa aaagaattga aaatgcactt atagatatta atataccaga
    20881 acagatatta tttgttccta tggatgcaaa attaatagaa caagttttaa ttaatttaat
    20941 agataattct ttgaaatatt caaaggaaga ttgtaagata gagataaatg tatatgagaa
    21001 agatgattat gtttggtttg aagtatctga taatggtcct ggtatatcaa aggaacttaa
    21061 aaagcatatc tttgacagat tttttactgg agaagaagga gctaaagatt ccagaaaggg
    21121 tgttggactt ggattatcaa tttgtaaatc tataattcaa gctcataaag gtgaaattat
    21181 ggtagaaaac aataaagata agggttcaac ttttaagttt agtttgccta aggaaaatga
    21241 atagttttaa attttaaaat tagttttgga ggaagaaatg agtaaaaaac ttatattgtt
    21301 agtagaggat gataagacaa taagaaaatt tatatctact gcacttttaa cacaggatta
    21361 tgatgtcaag gaggctataa ctggaaaaga aggtatatct atagctgtct catattctcc
    21421 agatgttgtt cttttagatt taggcttaga agatatggat ggaatagagg taataaaagc
    21481 tatcagacaa ttttcaaata taccaataat tgtagtttca gctagagagc aagatagaga
    21541 caaggtagaa gtatttgatg caggggcaga tgattactta acaaaaccat tctccatagt
    21601 agagttatta gcaagagtaa gagtagcatt tagacattca caagtagaag ctcaacaaaa
    21661 agatgatgtt aaatctacgt ttgaagtaga taaattattg attgattttg ataaaagaaa
    21721 agttattgtt gatgatgttg aagtccattt gacacctatt gaatataata ttctttcatt
    21781 acttgcaaag tatcatggaa aagtgcttac tcataatttt ataataaaag aaatatgggg
    21841 tagtgttata gggaatgaga ctaagtcttt aagggtcttt atggcaacgt taagacgaaa
    21901 aatagaaaaa caacctgcaa acccaagata tataattaca gaagttggtg taggatacag
    21961 attaaatgat gaatagttgt aatatattaa ttattagatt atgtgcttat taatatatat
    22021 taaaattata tagtttagtt aaaataagaa tgaagtgtat gtgcttgttg tcagtttagc
    22081 acatgcattt tattttttag cttttttagt atcaataatt ttttagtatc aataatatga
    22141 aatatgtttt aaaataagta tatagtgtaa tatgtaataa aaactttggt taaa
//
LOCUS       DAMJYX010000058        22004 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJYX010000058 DAMJYX010000000
VERSION     DAMJYX010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22004
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.058"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(77..1183)
                     /locus_tag="PC479_002281"
     CDS             complement(77..1183)
                     /locus_tag="PC479_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2804822.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(1218..2759)
                     /locus_tag="PC479_002282"
     CDS             complement(1218..2759)
                     /locus_tag="PC479_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2804823.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(3121..3867)
                     /locus_tag="PC479_002283"
     CDS             complement(3121..3867)
                     /locus_tag="PC479_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2804824.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(4079..5389)
                     /locus_tag="PC479_002284"
     CDS             complement(4079..5389)
                     /locus_tag="PC479_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2804825.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(5402..5872)
                     /locus_tag="PC479_002285"
     CDS             complement(5402..5872)
                     /locus_tag="PC479_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804826.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(5876..6727)
                     /locus_tag="PC479_002286"
     CDS             complement(5876..6727)
                     /locus_tag="PC479_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2804827.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            7651..8562
                     /locus_tag="PC479_002287"
     CDS             7651..8562
                     /locus_tag="PC479_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2804828.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            8737..9234
                     /locus_tag="PC479_002288"
     CDS             8737..9234
                     /locus_tag="PC479_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2804829.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            9978..10340
                     /locus_tag="PC479_002289"
     CDS             9978..10340
                     /locus_tag="PC479_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2804830.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            10405..11022
                     /locus_tag="PC479_002290"
     CDS             10405..11022
                     /locus_tag="PC479_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2804831.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            11019..11504
                     /locus_tag="PC479_002291"
     CDS             11019..11504
                     /locus_tag="PC479_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2804832.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            11547..12065
                     /locus_tag="PC479_002292"
     CDS             11547..12065
                     /locus_tag="PC479_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2804833.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            12201..13160
                     /locus_tag="PC479_002293"
     CDS             12201..13160
                     /locus_tag="PC479_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2804834.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            13186..14889
                     /locus_tag="PC479_002294"
     CDS             13186..14889
                     /locus_tag="PC479_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2804835.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            15150..17252
                     /locus_tag="PC479_002295"
     CDS             15150..17252
                     /locus_tag="PC479_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2804836.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            17265..17705
                     /locus_tag="PC479_002296"
     CDS             17265..17705
                     /locus_tag="PC479_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2804837.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            17741..18544
                     /locus_tag="PC479_002297"
     CDS             17741..18544
                     /locus_tag="PC479_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2804838.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            18584..19180
                     /locus_tag="PC479_002298"
     CDS             18584..19180
                     /locus_tag="PC479_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2804839.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            19286..20149
                     /locus_tag="PC479_002299"
     CDS             19286..20149
                     /locus_tag="PC479_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2804840.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            20216..21205
                     /locus_tag="PC479_002300"
     CDS             20216..21205
                     /locus_tag="PC479_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2804841.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            21297..21908
                     /locus_tag="PC479_002301"
     CDS             21297..21908
                     /locus_tag="PC479_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2804842.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8533 a         2595 c         3706 g         7170 t
ORIGIN      
        1 actctaatat ataacttata aagttgagga tacataggtt tttattttta taatttaaag
       61 agttctatta tgatatttat aatttaatca tatgataaac tattttggca gtatcatata
      121 attcgtctat atttacatat tcattaactg tgtgtacatc atacatacct agacttaata
      181 tagcacaatt atatccaaga ttagctaata tatttgcgtc acttccacca ccaattacca
      241 ttggatttgg aacaacacca acttgtctta ttgcttcttc acttaacttg aaaacatgac
      301 tatctctact aagttcaaaa ctagggtaag acatattatg ttcaaaagta taagtagtat
      361 taaatttaga tgcagcgtcc ttacaacatt tttccatatg attaagctca tactctaaag
      421 tttcaggtat atgagaccta atttcagcag tcaatgtaac cttatcagtt actatgtttg
      481 tagctccacc accttctatt tcacctatat ttgaagtagt caatgaatct attctgccta
      541 tgtgcatgtt gcttattgca tgagaggcta ctaatatagc atttaaccct ttttcaggtt
      601 ctataccagc atgtgctttc tttccttgaa aagagatttt tatagattgt tgagcaggag
      661 ctttgtatgc tatcgaacca atagctccac cagaatctaa aatcaccata tctttacaag
      721 gcaagttgtc aggattaaag tttttgacac catgcatacc agcttcctca cagattgtaa
      781 aacaaagata aatatctcta tgtggtattt tttctgtaat aacatgttct aaagcttcaa
      841 gtatagcagc tattccagct ttatcatctg caccaagtgt agtagttcca tcacttctaa
      901 ctacattacc atctagtatt ggttttacac ctaaacaagg ttgaacttgg tccatgtgtg
      961 cacataaaca aagtggtcta cttccttctt caccttttat ataggcaatg acattacctg
     1021 tattaccacc atatttttca cctgcatcat ctataatggc atctatattt ctttctttta
     1081 agtagtttac caaccactta gacatttcta attcttgatt agatgggcta tcaattctaa
     1141 ccatatccat aaaattattt ataagtctat ttttatctaa cataaattat ttctcctttt
     1201 agaaagattt aaataaatta catgctatat gaaggaagta ttccaggtcc tagtggaata
     1261 ttaaatacca taaacaatat taatagtata gtccacacta ttaagaatga tatagagtat
     1321 ggtatcatat tagcaattac agtacccata ccagcatctt tatcgtattt tcttgtgaat
     1381 gctagtataa tagggaagta cggaaataat gggctaagtg gatttgttat acaatcacct
     1441 atacgataag ccatctgagt aagtgctgga ttgtatccaa ccatcataaa cattggtaca
     1501 aatataggtg caagaatagc ccactttgca gatgcactac ctataaataa atttataaaa
     1561 catgataata taataaatcc tatcaacaat aaaggtccat ttattcctgc tgattttaaa
     1621 aattctccgc cctttacagc tagtatcaga cctaaattac tatttgtgaa taattgcaaa
     1681 aattgtgaag cagcaaatgc tagtattata tatccaccca tatcactcat tgcactagcc
     1741 attatagaga ctacatcctt atccttcttt attttaccta tagtttttcc gtatacaaca
     1801 ccaggtatga aaaatgctag tgctattata ggtaccatac ctttcataag aggagcattg
     1861 tttgatagta aatctcctgt ttctgggtca gctaagaatg gttttttacc gattacacta
     1921 agagctacta taatagccac atatattaat atacttatac tagctttttt aagccctttc
     1981 ttttctacat cactaagact tccatcaaca tctagttctg catttccttc atatttacca
     2041 aatctaggag ctacaatttt ttctgttacc catgttgcca aaagtattag aacaaaagtt
     2101 gatgctatca taaaaaataa gttcattgtt gcattggctt gaaatccaga atcaatcatt
     2161 tgagctgctg gaactgtaaa acttgcagca agtatatcat tgacacttat cataatgttt
     2221 gcggcaaatc ctagacagac tgctgcatat ccagaaaata aacctataag tggatgtcta
     2281 ccaacaccaa gatatacaag tgcagctaat ggtggaaata atattgtacc tgcatctgaa
     2341 gctatattgg caagcatacc tatagtcatt acaacaagag ttactcttgt ttcaggaatc
     2401 ttgcttactg atgctttgat tgttgctgtc ataagtcctg ttttgtcaca tacaccagca
     2461 cctatcatag ttactagaac tagacccaaa ggagcaaacc cttggaaatt gctgactatg
     2521 tttcctaaaa gaatttgaag ctgttctaca gtcaataaat ttacagcggt tatagtttct
     2581 ccagtaccag gatgaactac tgatataccc atagaaccaa ctagactaga tagtataagt
     2641 aaaagtacac ataaccctaa aaatattgta actgggtctg gtagtttatt accaacagtt
     2701 tctattctgt ttaatgtcct gtcaaaaaag tttttcttct taactacgtt atcattcata
     2761 atatcctccc taatgtttaa aaatataatt aaaaaataag tattgtggtt aatcttacaa
     2821 tttaaattct gtttgtcgat ttaagacgtg tccacaatat gtcctaaaaa aagtataaaa
     2881 taaacttttt cctgtgtcaa tgcagttttc tgaattttct ggttgagtgg tgttagttaa
     2941 atatgcttta agtagattaa tgacgaattt tatctaatgt atttaaataa catatgcact
     3001 ttaaaattaa ttttaaaaca aaattttatg aaaagctagt ggaagattaa gttattaaaa
     3061 taagtagcgt agtaaagtgt tagatttttt aattaatcaa ataaatttac tttgtatttt
     3121 ctacaaaaat ctgtgaatat cttttgaatt atgtagagga tataaattta ttttatttac
     3181 atagattaga ttttgttcta atttactgta ttcaaatggt tttccatgtc caggataaat
     3241 cattttaggt tttgtattga tgattgtttt ccaagattga ataaattcta gtttgctttc
     3301 tgcaaatatg gtaattttgt gagtacttgg aaatccattc attgcagaat ctccacaaaa
     3361 taaaacacct tcatcaatta gtagtgaaat tgaatcatca gtatgtcctg gtgtatctat
     3421 aatttttcca tttagttctt tttcaatttc tttacgattc ttgtcagaca caagaataca
     3481 ccttttttca aattcttgtt taagtggaga aaaactatgc tcaccttttc caaatagttt
     3541 cataatttgg caaaagagaa aggcagtacg gctggtacaa ccaccaataa aagagttttg
     3601 acctttatat agaatttcta aagctttatc atgcataaca atctttgtat taggagattc
     3661 gtctagaatc tgatttagaa aaccaacatg gtcatcatga gcatgagtta gaaatatgta
     3721 tcgaatatct ttgattttta tgttgaattt agaaattcgt tttttgaaat gtttaaaacc
     3781 attttcatat ccagtatcaa tcaagacaaa gccatcttga atagggtaca accatgtatt
     3841 tataattcga tttcctgcat taaacatttt ttcaccacct tgaattagaa tagaaattta
     3901 tattaattag aaagaatata gcatagttct actgatattt caacgtagct ctaaaagttt
     3961 taaggaggat aatatatttt agtttaatct aaaaactaaa agaaaagaag tatactgtta
     4021 caaaaacaaa gagtggtata acttttacag tagataccac cctattataa aaatagactt
     4081 ataaagtttt atcgtgtttt tctaagaatg ttagatattc ttctttagtc ataactggag
     4141 taaaggtatc taatatatct ttagcatcct tagcattatc aaaaagaagg tctacaactg
     4201 taagtgccat tgctttagca ggaattatgt aagctaaatc ttcatcaact atttcaaaat
     4261 ctcttgtatg aagagctcct tttacaccac caatcatagg gtgaagagta ggcattatat
     4321 gagacacatc tccaaaatca aaagagcctg taaaatctcc accatcaata acttttcctt
     4381 ttagacctaa atcttcaaga ttgtttttaa ataaattatc aagacttttg tatcgtaata
     4441 ttggaagata tccaggaatt tcagttatct caacatcagc tccaactgcc attccaccag
     4501 ccatcaaagc cttatttatt ctttcattag catcaatcat accatttata gttctagctc
     4561 taacatatga ttccatatga acatcagcag gaacaacatt tactatatct ccaccttttg
     4621 tgatgatagg gtggaatcta actctttcag actctttaaa agtttctcta agtgcattca
     4681 cgttgtttat agcaagcatt gctgcattta aagcatttat tccatcatgt ggagcagaac
     4741 cagcatgaga ttctttacct ataaatttaa ccttttttcc aacaaatcca ttactctcag
     4801 gaccaactag agccttatct tctcctaaat cagaagcatg aaacatcata gatatgtcta
     4861 tatcatcaaa ataacctctt tttattaatt cttgttttcc accaaaataa gttatttttc
     4921 cttctttttt taattgctct ctgtattcca actcaataaa ctcttcagca ggagtagcca
     4981 taaaagatac tttaccatct aaatgttcta aaacgccact ttttactaaa ccaacagcag
     5041 cacctaacat tccagctatt tgaatattat gtccacaagt atgagaagca cctatttcat
     5101 tagcatcttt atgttcttta catgatatac catccaattc tccaagtata gatatatgag
     5161 gacctttctt gctagaattt gcatctgctt tgcatcctgt aactgctata tttttttcta
     5221 cagataaatt taattcttgc tcaaaaaagt ttgaaacagc ttctgtagtt ttaaattctt
     5281 tatatccata ttctggattt ttgtatattc cttgacctat ttttaatatt ttttctctat
     5341 tttcatctat agtttttatt actagagatt ttaattgttc tttattcata ataaattcct
     5401 cctatatttt accttgaata cttaaaacta tatgagctat actcgcacat gcaaagaata
     5461 tagttgtaaa tacaataagt gaaattataa ctattttcca tgacatttct tttaaagctt
     5521 ctattttgtt tccaacagaa attcctgcaa aagctagtag tggagtagtt atagccataa
     5581 agttaatatt attagtgtta gttaagaata cttcagatgt tggactatat ggcatactaa
     5641 gtaaaaggcc gattatagtt gcaaatccaa aagctggaaa gattgattta ggaaataaat
     5701 cttttataat catagctgcc atagcagcta atattagcat caccatacca ggaagagctg
     5761 taactataga tgtaccaacg ctaattcttt gtcctataag aattataact ccaataacag
     5821 acataaccaa taattgatat gaacttttct ttaaatttaa attagcaaac atttattagc
     5881 actctccttc tgtagattta atttctttag tttttttatt tctcgaaaat ttaggttcta
     5941 aaatcttata cataaaatta gtaaatggta aggatacaaa aactaagaat ataattcctg
     6001 ttatactaga catcatattg cttgttgcag cataggcaag tattgtatct gccatttcag
     6061 gtggtaccat ggtagataat gaaccagcag ctgctgtcat catacttcca ctaccaacac
     6121 cacaggccat tcccaaagca tatgggtgaa gaccactata aatagatatt gaacctagta
     6181 cactaaagta tatagtacca agaactgttc ccattaaata tgtaccaagt acaccactac
     6241 cttctggtga gtttatccca tatttttctg atataactcc taaagaaggt tcacgactta
     6301 tacttgcagt agctccaaca gcttctcttt tcattccaaa cattaatgca agaggtaatg
     6361 caacgattgg tgcaagtata tgaccaaatt cttgagcgat aaacgctggt ccagcttgaa
     6421 ttattttgtc aatgtttggt ccaactgtag taccatattt tataccaagc attactagtg
     6481 caattccaac aacttcacca gcaatattta tttctttttc tccaaccact tttttaagtg
     6541 ctttaatttt tttacctaat aaatctggtg ttattataag tccaattata actgcataaa
     6601 gcatagggaa cagtactata gaggcttttc caatagctat agttctttgc cctataaatt
     6661 cacatattac taccaagatt aagcaagtta aaaatacttg aataaaatgt tttttattaa
     6721 tttccatgat ttcctcccca aaagaataaa tattttactt atctgaaatg agaaaatacg
     6781 ttttcttaag tataatacgt tttcctaaat aaaatgaaaa aactatttca aataagtttc
     6841 agaaatgtca ataacatgtc cttaattaag gataaaacaa aaatttaaca ttgtcaacga
     6901 aaaatatcta aaatattaca tattttgaat atattgtaat atagctttat tatgaaaaaa
     6961 ttgtttaaat attgtaggaa taatagctaa aaagctttaa aattagtaat ataaaattga
     7021 aaaataataa tatagagttg agaaatagta aattgaaatt ttaaaataaa tgagtggaag
     7081 tagcttagta aagtatggaa atgaatatta caatagaaaa taaataatat gtaaagactt
     7141 taaattaatt ttaagtatta agtacaaata ctaattttga tatagaaatt gaataaagta
     7201 aatgataata agcaaatgaa ttaagatgat aataagtgaa ttgatataga aataagtaaa
     7261 ttaagtaaga cgataacaag tgagataata ataaatgaat tgacatagca ataagtaaat
     7321 taagtaagac gataacaaat gagatgataa taagtaaatt gatatagaaa taagtaaatt
     7381 aagtaagatg ataacaagtg aggtaataat aagtgaattg atatagaaat aagtaaatta
     7441 agtaagacga taacaaatga gatgataata agtaaattga tatagtaata aataaattaa
     7501 gatgataata tccaaagtga gaaagataat agaaagtaaa taaaatcatg taaaaataat
     7561 ttagataaga ttctaagatt ttatttattt aaaaattagt atgattaatt tagattaaat
     7621 aaatttaata attttagtag gtggtaatga atgaaaataa atagattaac agaaataata
     7681 gtaattctat taaataaaaa attagtaaca gctaaagagt tggcagatag atttgaagtt
     7741 tctacaagaa ccatatatag agatatagaa actctgtcta tgtcgggagt accagtatat
     7801 atgacgaaag gcaaaggagg agggatatct ttaatagaag aatattctat agacaaggct
     7861 atattgtcaa aaaaagataa agagagttta atagttgcac tgaaaacatt acaggcgact
     7921 aagtatccag aaataaattc agtagtaaat aaaattggct caatatttgg agagcagaat
     7981 ttttcaaact ggattgaaat agactttact gaatggggaa gtaattttaa tgaagatgat
     8041 aaatttacaa aaataaaaga agctatttta aggcgtaata ctataaattt taattatgta
     8101 aatagtttgt caagccagac aaacagaact gtcgaaccac tgaagcttat gtataagagt
     8161 aagacttggt acttgtatgg attttgtaaa ctaaaagatg attttaggat atttagaatt
     8221 agtaggataa ggaatttatc tataaaggat gaagtatttt caagaaagat aatagaagaa
     8281 gtatgtttga atgattctaa agtaattaag gaaaatacta taacgttgaa attgaggttt
     8341 aaagaaaaga tgctttttag agtatttgat gattttaata aggatttaat aactaaaaat
     8401 gaagatgata catatgatgt aattacagaa tttcctattg gtgaatggat ttatggatat
     8461 attttatcat ttggtgacaa tgttgaagta cttgaaccta aagatgttag ggataatgta
     8521 ataactagac taagggagtt gtctaaaatt tattcattat aatataatgc tagattaaag
     8581 attttattaa atatataata tcttgcattt agtatataat atctcactga gtttaattat
     8641 attaaaaata tgacataaag gtgtcgtata ttataaatta taattgaatt agttcaaaat
     8701 aaaatagatt aataaaaaca aagtgaggta taaagtatga attatgaaat agtagagata
     8761 aatgagaaaa tagttatagg aatatcgaag gagactacaa ataaagatgg acaagcggta
     8821 aatgatatag gtgaattgtg gaaaaaattt atgggcaaag ggatatataa tgctataaag
     8881 ggtaaaaaga atgacaagac aataggtcta tatacagatt accagggtga ttttacaagt
     8941 ccgtatagct ttgttgcatg ttgtgaagta aatagtaatt ctgataaaga gaagaattta
     9001 gaagaattaa acactaataa tactgttgga gaaagtatta ttagtaaagt tataccagca
     9061 ggtaagtatg caaagtttgt tatagtagga ggtcaaaaag aagtaggaga tttttggttt
     9121 gagttttggc aaatggattt tgataggact tatataagtg attttgaaga gtatcaatgt
     9181 aatacttttg atacaaagaa acaagaaatt catatttaca taggaataaa gtagatttgt
     9241 tattactata ttgaatgaaa tattgagtat agtacattta ttatagatag ttttattatt
     9301 tataactata agcagtaaat ctgtaaaaga tttactgctt ttttagtaat taatagtatc
     9361 agtatcactt tgaatcttca gacatattct tcatttaata ttgtttattg ataaatgata
     9421 cttgtaaata taatgatttg ttatttaatt taaaatatga aaaaaagtat tataaacaca
     9481 ttagaatact tctggattta tgagtataca atttgaaaaa tataaaaaat aatgtataaa
     9541 gctaaatcta atttaatcga taatacatat agagaaatat aattaagtaa ataaacaact
     9601 atctattaga tattgagaaa gtcaaatcct atatgaatct acagaaatga ttttaatgaa
     9661 tctgaaaatt ttttttatac aaacataaaa aaggtataaa gttgttttat tataatttaa
     9721 ttacttttga taacttttta tgttttatac aattatattt ttaataactc gtcaaaacgc
     9781 caaacatata ttacaataga tatataagtt tttttgttga caaattatgt taagagaatt
     9841 aagtataata tggcataaat aattactttt atttatcaat gaaatgtatt tattgtgtag
     9901 cgaaaaatag tcagatagta tagacaataa ctgaagataa aagcgtttat tgttgtacat
     9961 atggaggagt tagttttatg gaaaataaaa ttttgatagt agatgatgct atttttatga
    10021 gaatgataat taaagaagct ctgagtaaaa gtggctataa taatttaata gaagcaaatg
    10081 atggagaaga agcgtgtaat atatttaaat atgaaaagcc tgatttgatg ttgcttgata
    10141 ttacaatgcc aaaaaaagat ggtttagagg tgctaagaga aataaaaaag ttggattcaa
    10201 gtgctaaggt tgtaatgtgc tctgcaatag gacaagagaa tatgattgta gaagccataa
    10261 aacttggagc gttagatttt atagtgaagc ccttcaaaac agaaacatta gttaaagttg
    10321 tagataatgc tttaaaataa aagtgtaaat atacactaat acaatgaaaa gcaaataaat
    10381 gcaataataa agggaggggt ttatattata aattatttag aacttgatga tgttcatata
    10441 gatgctctta gagaaattgg taatataggc tctgggaatg caattactgc acttgcatcc
    10501 atgataaata gcaatgtaga agtattaata cctatggtca agatacttga atataatgaa
    10561 gcgacaaatt tgcttggtgg tccagaaaac aaagtagtat gtattttact cgatatgaaa
    10621 ggtgatatca atggaatgtt tatgtttttg ttggatgaat caataactca acttatgcta
    10681 agttctcttt ttaataaaga agaagctttt ttagatgaga ttgaggcaat tgaaatttca
    10741 gcaattaagg aaataggaaa tattatggca agttcatatg taaatgctat tgcatctatg
    10801 ttaaacatga ctataagtgt ttcaattcca gatatatgta ttgatatggt tggagctgtt
    10861 ctaaatgtac ctatgataag attttcagat gttggtgaca aagtcttatt tatagaaaat
    10921 aaatttaaaa tgagtgataa ttattttact agtcacattt tgatgattcc agaaatgagc
    10981 tcacttagag aaatactcgt gaggttggga ttagaagtat gaatagagag atagtagtga
    11041 acattgcaga tatgaagata gcctatagac caaatgtttt agttacttat gcacttggtt
    11101 cttgtgtagg agtatgtttg atagataaag tagctggaat tggtggaatg ttacacgtta
    11161 tgttgccata tagtaaagat gctattaaca ttgaaaataa gtgtaaattt gctgacacag
    11221 gaataaatga gctcattaaa tctatggaga atataggtgc aaatagaata tatataaaag
    11281 caaaaattgc tggaggtgct cagatgtttt taggaagtag caattcagta attgcaagta
    11341 taggacatag gaatgttgag gcagtaaaaa aaactctttt aggattaaat attccaatat
    11401 tagctgagga cacaggtatg aattatggta gaacaatacg tttttacact gagacgggtg
    11461 aattacttgt agattcaata aataaaggta agaagaaatt ataatatata tttcatagaa
    11521 agtgtatttt aggagggagg tattaaatga gatttccaga tgacccatat ttagattatg
    11581 gactaattga tgaaggaatt aagtatctaa agtttaaagt agatgagcaa gattttgggg
    11641 ttgaacttga aaaggtaata gaaattactg gaaatcaaga aattatattt attccagaac
    11701 tacctacata ttctaaaggc ataataaatc taagaggaaa aatcattccg ataatagata
    11761 tgcgtttgag acttaaaaaa gatgaattaa tgactacaaa ttgcatgtat atagtagtaa
    11821 ctgaaataaa ggacttggta gttggatttg tggttgatag agttgatgat atagtaagct
    11881 tagaaaaaag taaaatatca cctccaccaa gagttactat agaatattcg gattattttg
    11941 taagtggagt tggagaatta gaaggtaaaa taattacttt actagatttg gagaaacttt
    12001 tgacagataa agatgaagtt ttaatagatg aattaatcaa tgggtttgat tgcactatca
    12061 tgtaacatag ggatgtagtt ggagtgttat tataaaaaga ttgtgatgag aacatttaag
    12121 aatatagtgg tataattatt aagtataaaa attttaaatc attaaaattt gaatatatta
    12181 tgtttaagag aggtttgtat atgaaaaata agcaacaggg aacaatttta gtcgttgatg
    12241 atagtgttct gatgtgtgac ttaatagata aaacacttac aaaagataat tttaaggtta
    12301 aaaaagcatt taatgcaata gaggcaaaag aatttattga agaatttata ccagacatta
    12361 ttttgcttga tataatttta ccagataact ctggttttga gttttgtaag gaagtaaaat
    12421 ctaatgctcg tacagcagat atacctataa tttttataac ttcaaaaaat acagatacag
    12481 acatagtaaa aggatttggt gtaggagcaa ttgactatat ggtgaaacct tttagtatga
    12541 cagaattaaa agctcgtgtt atagctcatc ttgaagtaaa gaaatctcaa gataaattaa
    12601 aaaagataaa taatgaactg gaatcttctc ttgaaaaatt gaatagactc gttgtaagag
    12661 attatcttac aggtctttac aataggcgac atataattaa tcagcttatg gagcaaagaa
    12721 gaattaataa atatagtgaa acatcaatat cccttatcct tggtgatatt gatgacttta
    12781 aggttatcaa tgatacttat gggcatgaag caggggattt cgtgttaaca gtaatatcaa
    12841 gtataataaa aaagaattgt agacaaattg atactgtatc aagatggggt ggagaagagt
    12901 ttttgataat gctcacaaat actccaatag aaggtgctaa gatactttca gaaagaatcc
    12961 gtaaagagat aaaggagttt gatttcaact ataaagaaca taaaataaag tgcactatta
    13021 cattaggagt agtagaagta aactcaaata tatcattaga agaaaacata tccttagcag
    13081 ataaagcaat gtatgagggt aaaaacttag gaaaagattg tagcgtagtg agtacaatga
    13141 aaggattaaa aaagatataa acttacttta tgaaggagta cagatatgaa aagagaagta
    13201 aagctttcta aattagcaat tgtattgctg attattatag tattggctgt tctagcaggg
    13261 attttttctt ctatttcatc ttataaagca tttgaaaaag tagaaaaaag tattaatgat
    13321 atgaataatt tgaggactca aagtcagtat ataaaagata caactagaga gataagtgat
    13381 gtattaagaa gatatgtatt tacaggagaa aaaaagaata aagatattta tgaaaaagaa
    13441 ttatacggaa aaacaagaga atcagcttta aacaaattag aaaaaattgg tcttacaaag
    13501 agcgagtcaa ataaaatctt agaagcaaaa gaaatatcag attctttagt accaacagaa
    13561 caaagtgcaa taaaggcagt cgaaagtaat gataagagta aagctcaaag tataattttt
    13621 ggtagtgact atataaactc ccaagagaaa atggattctt taataaatga atttcaagag
    13681 gatatagtta cccgttcaaa ttccaaaatt gtagaagtaa aaaaagaatt acaaagtcta
    13741 atattaagaa caatatttat actttccctt ttaataatat tgacaataat agaatataca
    13801 gtaataaaat taaaaatagt gattcctatt aataaagtag aaaaacactt ttcaagaatt
    13861 gcatctgggg acttaagaac aagtatagac gttgaagaaa gtaaatctga aatagggcta
    13921 ttagtgagtt ctgtcaaaaa gatgcaaagt atgcttttaa attacataga ccttatagac
    13981 agtacattaa ctagtattgc aaaaggagat ttaagagtag agatagacca agaatttata
    14041 ggtgatttta aatcaattaa aatatcttta gaaagtataa taaactcttt taatggtgat
    14101 ttttctgaaa taaatttatc agcagagcaa gttgcaagtg cttcagaaca agttgcagca
    14161 ggtgcacaag ctctttcaca gggtgctact gaacaagcta gttcagtaga agaattagct
    14221 tccacgatta atgagatatc agatggaatt aagaagtgtg cagatagtgc tagtcttgca
    14281 tgtgaggtat ctgaccaatc tgcatcacaa gttggttttg gaagtagatg tatggatgat
    14341 atgatgacat caatgggtga aataagtgaa aaatcgagtg aaatatcaaa aataatcaaa
    14401 acaatagatg agatagcatt ccaaactaat attcttgcat taaatgcagc tgttgaagca
    14461 gcaagagcag gtcaatatgg tagaggattt gcagttgtag cagatgaagt taggtctttg
    14521 gctggtaaaa gtgcaaaagc agctcaaaat acagctattc tcatagaaga agctattaaa
    14581 acagtagaaa atggtgtcag aattgctaaa gagactgcaa atgcattgga cttggtagta
    14641 aatggtgtag aaaaaactac taattatatt aatggtattt cttcggctgt acatagtcaa
    14701 gaagtagcaa ttacacagat tttatctgga atagagcaaa tttccatagt agtccaaatg
    14761 aactcagcaa cagcagagga aagtgcagca gcctcagaag agatgtctgc tcaggctcaa
    14821 attttaaaag aattagtaga aaaatttcat ctaaaggaaa gtgaagtaag agaatcatca
    14881 tgggtttaaa ttatgaggtg aatattgaag taagagaatt atcatgtatt taaattatga
    14941 gatgtatata tattgaaata agagaattat caaggattta aattatgaga tgtatatata
    15001 ttgaagtaag agaataattg tgtatttaaa ttatgaggta catatagtga gataagagaa
    15061 tcatcaagga tttaaattat gagatgcata tataatagaa aattatttgt aggtcgtagg
    15121 tgataaatgt gaattatatt gaaccgagta tgagacaaat gttagatatg tttatatttg
    15181 agacaagttt attactcgaa caattagatg aaattatgat tcaaatagaa aaagagaaag
    15241 tatttactca taacaacatt aatgaaactt ttagaataat gcatacgata aaaggttcag
    15301 catctatgat ggggttagac aatatatctg ctttagcaca tagtgtagaa gacttgtttt
    15361 atgctatacg tgaaggtaaa atagaagttt tagacaatga cctattattt gatttagtct
    15421 ttcaatcatt agattttata agaagagata tagatgagtt acaatcaaga ggttgtatat
    15481 cttctgatta ctctaatttt attgaaggaa ttgaagagta tgtgagtact ttggaattat
    15541 caagttctat gaaatcaaag gataatgcaa atttagaaag taaagacgaa aaagaagtaa
    15601 tcgattcaaa ttttgaatac aaagaagact ctgatgtagt aaaaataatc tttgaacaag
    15661 gttgcatgat ggaaaatgtg agagctttca tgcttgtatt tgaacttaga gattactgtg
    15721 aatttataga tttttatcca aaagatgtag aaacaaatgc agagtctaca gagtatatta
    15781 aaagttatgg gttttatata caatttgtag ctaaagagga taaggcaata gtttataaaa
    15841 ctatagaaga taatctctat gtaaaagaat acataaatat tagtaaaaaa gaaatagaaa
    15901 tgaaaagtac atcaaacaat gatgcagtag aaaataaatt aaaagataat aataaagtaa
    15961 gtaaaagtta tcaaaataac tatacagaag atgagataca agaagatgtt ttaaatgaaa
    16021 aaactgatat ggaaaaagat agtctaagtg aagaaaatga acatttacca ataagtaagc
    16081 agagtattat gagtgtaaat ttagaaaaat tggacttact tcaaaatata gttggagaaa
    16141 ttgtaattat ggaatcaatg gttgtaaata gcgtaaattc tacaggtgtt gatatagata
    16201 atttcaataa ggcaacaaga caattacaca agcttacaga tgaattgcaa gatatagtaa
    16261 tgtctatgcg tatgattcaa attgctggtg tatttcaaaa aatgcataga attgtacgag
    16321 atatgaataa aaaattagat aaaaatgtag agttaataac tataggagca gaaacagagg
    16381 tggataagag tgtaattgat aatttagcgg aaccttttat gcaccttata agaaatgcta
    16441 tggaccatgc aatagagcct attgaggagc gtatagcgaa aggtaaagaa gaagtgggaa
    16501 agatagtttt atctgctgaa aatctaggcg gagaagctgt gataagtatt tcagatgatg
    16561 gtagaggcat tatgagagat aagattttgg caaaagctaa ggaacaaggt cttcttaaac
    16621 atcaaagtaa tgagtatact gatgacgagg tcaattcttt gattatgact ccaggctttt
    16681 ctacaaaaga atctataact gaattttctg gaagaggagt aggcatggac attgttcgta
    16741 aaagtattga aaaagttgga ggttcaattg aagtaaaaag taaacaggat gaggggacaa
    16801 cctttactat aaaaatacca ttaactcttt ctatagtaga gggtatgaag tttaaagttg
    16861 gagattcact atttacttta ccaatatcat ccataagaag aacatttaaa cttacagact
    16921 taaatcaaat aattacagaa ggaaataaga cagagttaat tacaatatat ggagcatgta
    16981 ttcccattat aaggttacat aaaatatata atattcaaac aaaagtaacc aatctgatga
    17041 atggtaatat gataattatt gaaaatgagc ataaagcagt atgcattttt gttgatgaaa
    17101 taataggtaa tcagcaagtt gttgtgaaac catttccagt ttattttaac cgatttaata
    17161 taaaaaataa gggtttatcg ggatgtacaa tacttgggga tggaagtata agtttaatta
    17221 tagatgcaga tagcattata gaaatatatt aggggattta aagtatggct aaagatgaaa
    17281 ttattaaaca agtattaaca ttttatgtca atgatgttat ttatggaata gaattagaaa
    17341 atgtgattga aaccataaga tttcaatcaa taacgtatgt accatgtctt ccaacatata
    17401 taagtggagt tattaatttg agaggaagaa taataccagt tattaatatg catataaagt
    17461 acaatcttcc taaagctgat tataatgaga gaacttgtat aataattaca aaggttgatg
    17521 agtatcaagt tggaattatt gtagataaag ttgttgatgt tattcatata gaaaatctaa
    17581 atttactaga gacgactaat tctaacaaca ctaatattaa taaagatata aaagagattg
    17641 cgaaagttga agataattct atattaattt tagatattcg aaagtttttg attaatagca
    17701 tatagttagt tctgataaat tagcaggagg aagtttttgc atgataaaac tcacagatga
    17761 agaatttata gttcttgtaa accatgtaaa aaaagaatat ggtattgatt taagtaaaaa
    17821 aagagcgtta attgaaggtc gcttatacaa tacaatgata gagaaaaaac ttagttcttt
    17881 tagtcaatat atgaatctat tatttaaaga taggacagga aatgaagcta taaacctaat
    17941 aaatagattg agtactaacc acacattttt tatgagagaa cctcaacatt ttgagtttat
    18001 acagaatagt atacttcctt tttgggaaga aaataataaa ataagaaaca taaatatatg
    18061 gagtgcagga tgttctagtg gagaagaagc gtattcaata gctatgactt tggatgatta
    18121 ttttggatat aataaagagt tgtggaacat taaaatcata gccacagata tttcaactaa
    18181 ctcacttgaa aaagctaaaa gaggtatata catagaatca aatgttaaca atgttcctga
    18241 gttatggaag aagaagtatt ttatagacaa caaggatggt acatttaaaa tttgtgataa
    18301 gattagaaaa agtgtaattt ttaagccgtt taatttaatg aatgatttct catatcaaca
    18361 ttttgatttg attttttgta gaaatgttat gatttatttt gatttaaaaa caagagaaga
    18421 acttataaat aaattttata atgctaccaa aaaagatagt tttttgtttg taggacatgc
    18481 agaagtaatc aacagaagta gtactaaata caaatatgta aaacctgcta tatataaacg
    18541 ataaaaaggt atttttgata tatgattttt tggaggcggt aatgtggaac gttttaataa
    18601 tatggttatt gctataggcg catctgtagg tggtacagag gcaatacttg aaattataaa
    18661 agacctacca aaatcaacac cagggattgt tgtagttcaa catatgccag ctatatttac
    18721 ttatatgtat gcacaacggc ttgacaagca gtgtattatg aatgtaagag aagctaagaa
    18781 taatgataga gtagaacaag gaaatgtgtt aattgcacct gggggttacc agatgaaatt
    18841 gtgtacggat aaacaaggat attatgtaac ttgtgaaaaa ggagaaagaa taagtggtca
    18901 ctgtccatca gtagacgttt tgtttgattc agtagcagaa gtagcaggta aaaattcaat
    18961 aggaataata cttacaggta tgggttatga tggagcaagt ggacttttaa aaatgaaaga
    19021 aaatggttca tttacaattg gacaggatga aaattcttgt atagtatatg gaatgccaat
    19081 ggtagctttt gatagaggtt ctgttatgtt acaattacct cttggtgaaa tttcaaactg
    19141 tttgataaga catattaata ttttaaaaca aaaagattaa ttttttataa ataaaaatta
    19201 atcttttatt aaattatatg gataaaaaac cctagaaact ccaaatataa taagagagat
    19261 ttatatttag gaggaaaatg ataatatgaa attttttaaa gtcgatacaa aagctaaagt
    19321 tttaagatat gtatttgttt taacattgac tataatggct gttttggcaa ttaaacatga
    19381 ctataaatat aataatttaa ggaaagatgc ttcaattaat ttaaatagta ataaggaagc
    19441 agtgactgtt atgagtaacc agtcattacc aaagggtaat acaaatagta aacaaacttt
    19501 atcagtaagt acaataggaa gtaaaacaat agtgaaaaat gatgagtatt tagaaagtac
    19561 tataaacatg cctgtaataa caaattcaaa taaaattgtt gaaaggtcta caaatgataa
    19621 gataaaaaat gacatatttg aattttataa taaatcatat aaagaagcaa agcagtatct
    19681 taaggataat cctgacgaga aaaataagtt tgttgcaaat gtagactttg aacttaaaaa
    19741 gaatactgat tctgctctta gtataaaagt gagatattat acttatagtg gtggagcaca
    19801 tggattttat caagatatag catataatgt ggatatgaga actggaaaat ttttagaatt
    19861 aatggacttg tttaaggata atactaaata taaagaagtt atagatgaag aaattaaaag
    19921 acaaatagct gaacttgaaa aaaaggatga agaaaatatc ggaatatata attttaaagg
    19981 tataaaagag aatcaaaact tttacttgca agatgagaat ctagttgtat attttgattt
    20041 gtatgatata actccatatg cagcaggaat accagaattt tcaataaata aagcgttgat
    20101 aagttctatg ctaaaaccag agtatgtaga tttatttgat ttgaaataga agattaaatt
    20161 aaatgttatg ctataatatt tagcatagag acttattaaa attgggggta taaaaatgct
    20221 acttttaaaa aaagacgaca taaaaaaagt atttacaatg agagatgcaa ttgaagctga
    20281 taaggaagct tttagaattt attgtgaagg aaagagtgta aatcctttga gaaccaatat
    20341 atctgttcca agtcaagatg ctagtatgtt atttatgcca ggatacgtag aagaattagg
    20401 ctgtggggga ataaaaattg tatctgtatt tcctaaaaat gctcaaaaag gcaaaccagt
    20461 aattccagca acagtactat tattagatgg agaaactgga gaagttagtg cagtattgga
    20521 tggtacttat gtaactcaga ttcgtactgg agcagcctct ggagcagcta tagacgtatt
    20581 ggctaaaaaa gattctaaga ttggagcttt gataggtcta ggaggacaag gtgagccaca
    20641 acttgaagcc atgatagaag ctagaaattt ggatgaggta agagttttta gtagaaacaa
    20701 ggaaagtaga gaaaaatttg cagaagaaat gaacttaaaa ttaagtaaat ataatactaa
    20761 aatagttgca gttgaatcat ctgacgaagc catagataac gcagatgtga tagttttagc
    20821 tactccatca aaacaaccag tacttaatgg aaacttagtg aaaaaaggag ctttaataag
    20881 tgcagtaggt tcatatatgc cagatatgca agagttgtgc ccaaactgtt taactagagc
    20941 gtctaagata ttttttgaat caacagatgc agttttatct gagtcaggag atatattaat
    21001 tccattaaaa gaaggtaaga taagtaaaga tgattttagt ggtgatttag gaaatgttat
    21061 aaacggaact attcctggaa gagaagatga tgatgagata attgtattta aaactgttgg
    21121 gataggtgta caagatgtcg taacagcaaa aagaatatac gataaagcaa aagaaaacgg
    21181 tattggaata gactggaagt ggtaaaatat tcaaaataat tctatagtta gacaaattta
    21241 tgttataatg ttgataaata aattttttat tattttaatt gagggaggat gtttgtatga
    21301 aaatggtaaa aaagttaata ttatctacga ttatggctct agttttagtt ctaactatag
    21361 ggtgttctaa tgcagaagaa aaagtaaagt cgaattttga agctaattta gctgcattag
    21421 aaatgtataa gtatgatgaa aaaggaattt ctgatgcatt aacagaagct gaatataaac
    21481 agattcaaac ttataacagc aaggacaagg tttcaatgat tgctcaattg acacctcttt
    21541 atggtaaaga aaatgctgaa aagatatacg aaacaattgt taaattaatt caaggtgttg
    21601 aaaagacagt aacagtggaa tcttctagta aggataagat aactttgaaa gttgtaagta
    21661 aagctttaga tactagtaaa gtaatgagtg aagcgaacac tttagtaaaa tcttatgtaa
    21721 ctgatgaaaa gaattacgat aaaaaagaaa aagaagttca agatgatatg acaaaaatga
    21781 ttatagacct ttacaataag aaaccaacta aggaagttac aactatgatt acatatacta
    21841 aaaaagatgg taaatggcaa ccacctagta atatacaaaa gttatgtgaa ggaatatata
    21901 caatttaatt tattttaaag acacaaaact atcttttaag catataataa aatgtttgga
    21961 agatagttta ttttattttc aaagtataaa ttatatttta taaa
//
LOCUS       DAMJYX010000059        21942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJYX010000059 DAMJYX010000000
VERSION     DAMJYX010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.059"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC479_002302"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC479_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2804843.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC479_002303"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC479_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2804844.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC479_002304"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC479_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2804845.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC479_002305"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC479_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2804846.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC479_002306"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC479_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2804847.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC479_002307"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC479_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2804848.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC479_002308"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC479_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2804849.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC479_002309"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC479_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2804850.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC479_002310"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC479_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2804851.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC479_002311"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC479_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2804852.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC479_002312"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC479_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2804853.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC479_002313"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC479_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2804854.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC479_002314"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC479_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2804855.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC479_002315"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC479_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2804856.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC479_002316"
     CDS             6989..7174
                     /locus_tag="PC479_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2804857.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC479_002317"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC479_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2804858.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC479_002318"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC479_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2804859.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC479_002319"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC479_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2804860.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC479_002320"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC479_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2804861.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC479_002321"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC479_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2804862.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC479_002322"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC479_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2804863.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC479_002323"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC479_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2804864.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC479_002324"
     CDS             11117..11767
                     /locus_tag="PC479_002324"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2804865.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC479_002325"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC479_002325"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2804866.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC479_002326"
     CDS             12536..12805
                     /locus_tag="PC479_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2804867.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC479_002327"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC479_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2804868.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC479_002328"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC479_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2804869.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC479_002329"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC479_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2804870.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC479_002330"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC479_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2804871.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC479_002331"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC479_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2804872.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC479_002332"
     CDS             14901..15848
                     /locus_tag="PC479_002332"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2804873.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC479_002333"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC479_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2804874.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC479_002334"
     CDS             16330..17163
                     /locus_tag="PC479_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2804875.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC479_002335"
     CDS             17151..18017
                     /locus_tag="PC479_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2804876.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC479_002336"
     CDS             18011..18814
                     /locus_tag="PC479_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2804877.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC479_002337"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC479_002337"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2804878.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC479_002338"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC479_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2804879.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC479_002339"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC479_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2804880.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="PC479_002340"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="PC479_002340"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2804881.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8816 a         2563 c         4494 g         6069 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aaatttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa tagggacaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMJYX010000060        21721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJYX010000060 DAMJYX010000000
VERSION     DAMJYX010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.060"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(78..563)
                     /locus_tag="PC479_002341"
     CDS             complement(78..563)
                     /locus_tag="PC479_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2804882.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(646..739)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1093..1476)
                     /locus_tag="PC479_002342"
                     /pseudo
     CDS             complement(1093..1476)
                     /locus_tag="PC479_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      1702..1799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1854..2030
                     /locus_tag="PC479_002343"
     CDS             1854..2030
                     /locus_tag="PC479_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804883.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            2256..2456
                     /locus_tag="PC479_002344"
     CDS             2256..2456
                     /locus_tag="PC479_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2804884.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            3208..3390
                     /locus_tag="PC479_002345"
     CDS             3208..3390
                     /locus_tag="PC479_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2804885.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            3461..4306
                     /locus_tag="PC479_002346"
     CDS             3461..4306
                     /locus_tag="PC479_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2804886.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(4530..4694)
                     /locus_tag="PC479_002347"
     CDS             complement(4530..4694)
                     /locus_tag="PC479_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804887.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(5236..5568)
                     /locus_tag="PC479_002348"
     CDS             complement(5236..5568)
                     /locus_tag="PC479_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2804888.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(5812..6453)
                     /locus_tag="PC479_002349"
     CDS             complement(5812..6453)
                     /locus_tag="PC479_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2804889.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(6434..6727)
                     /locus_tag="PC479_002350"
     CDS             complement(6434..6727)
                     /locus_tag="PC479_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2804890.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            7120..7179
                     /locus_tag="PC479_002351"
     CDS             7120..7179
                     /locus_tag="PC479_002351"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2804891.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(7695..8447)
                     /locus_tag="PC479_002352"
     CDS             complement(7695..8447)
                     /locus_tag="PC479_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2804892.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(8506..10161)
                     /locus_tag="PC479_002353"
     CDS             complement(8506..10161)
                     /locus_tag="PC479_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2804893.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(11434..12513)
                     /locus_tag="PC479_002354"
     CDS             complement(11434..12513)
                     /locus_tag="PC479_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2804894.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(12597..13253)
                     /locus_tag="PC479_002355"
     CDS             complement(12597..13253)
                     /locus_tag="PC479_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2804895.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            13575..13781
                     /locus_tag="PC479_002356"
     CDS             13575..13781
                     /locus_tag="PC479_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804896.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(13934..14362)
                     /locus_tag="PC479_002357"
     CDS             complement(13934..14362)
                     /locus_tag="PC479_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2804897.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(14838..15776)
                     /locus_tag="PC479_002358"
     CDS             complement(14838..15776)
                     /locus_tag="PC479_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2804898.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(16290..17102)
                     /locus_tag="PC479_002359"
     CDS             complement(16290..17102)
                     /locus_tag="PC479_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2804899.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(17128..17757)
                     /locus_tag="PC479_002360"
     CDS             complement(17128..17757)
                     /locus_tag="PC479_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2804900.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            complement(18101..19756)
                     /gene="ilvD"
                     /locus_tag="PC479_002361"
     CDS             complement(18101..19756)
                     /gene="ilvD"
                     /locus_tag="PC479_002361"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2804901.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(20080..20379)
                     /locus_tag="PC479_002362"
     CDS             complement(20080..20379)
                     /locus_tag="PC479_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804902.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            20639..21319
                     /locus_tag="PC479_002363"
     CDS             20639..21319
                     /locus_tag="PC479_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2804903.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            complement(21429..21686)
                     /locus_tag="PC479_002364"
     CDS             complement(21429..21686)
                     /locus_tag="PC479_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804904.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
BASE COUNT         7424 a         3518 c         2331 g         8448 t
ORIGIN      
        1 tttctacaaa aaagaatccc attaatggga ttcttttttg cgatttatta atatatttaa
       61 agcttgctag tatcgtatta taaacttaaa tgctcttcta tgtattgatt atatacatat
      121 ccagaatatc gtttttcatt tttattgata ttaactcttg cccattttcc aacgctataa
      181 catacttcaa ctttaaaacc atcagggatt gtagttataa ctttagattc taatgttcta
      241 tcatttctaa gttctaaact taatccattt gttcttatat ttcctatgta attctttttt
      301 ctatttttta ttgtatcatt actattgttt ttagcattac ttaatctttc taaatattta
      361 taaagtaacc aacctttgtc ataattactc tcaacttctg cccatattcc atctacacac
      421 tttaacttaa caattttacc ttctggtata ctttttaatt tcaaactatt tatgtctggt
      481 ttttctctta aattcaattt aattttagtc ttaaaattga tgctagattt gcgaaaattt
      541 tttaacatta attcagatat catcttaagc cctcctcttg ccaaatatta agttattaaa
      601 atatataact ctaaaaatac ttttaacaaa aagaattatt atctttttgg caactggctg
      661 acatgacctt tattacataa agattttagt ccctatagtt ttgcgtcatt agatttatct
      721 aatattgcct atatctgaat aataacataa tttgaacaat taaaaaataa tattacctca
      781 aattttatat tatttaaata taaattaaat ttttaattaa taataatcaa aaaatatctt
      841 tatatttatt caattactgt tctttatagt caatataaaa gacagtaatt tatctatagt
      901 gttacttata ttttatatat atggctattt ttcaacattt tacaatatat aacatttatt
      961 tttttaactt ttgtcagtaa aatgtcagca aaacacttta tccacaattt ttatataagt
     1021 tttccacaac ttatcaccaa tattatccac ataaaaaagg tagcacttat attattagct
     1081 accttttatt tattaatctt tatatttcca gctttttatc caacttccta tttctattat
     1141 tttttcttca gttatttctt ctccatcatg taattttgaa attaattctg ataaaggatt
     1201 gttatgtaaa cctctaaaaa ctctttttcc ctctattttg agatattctc tctcttttat
     1261 taatactgta tagtgtacat gcctcccaat tctctatgat attaaaaaac cttttttggt
     1321 taatctagct aaaagtataa ttgtagttgt atgcttccaa ccatatgttt tttctatttc
     1381 atctgcaaca tcttttgata ttacagtttt ataaccagta tgccatatgc atttcataat
     1441 ttttaattct gtatctggta attttttatt tagcataata tattccttct ttcaatttat
     1501 gtcacttctt caaatttatc tagcttaaat tataatagga gctttttaga acaaaaacta
     1561 tatttaattt ttaccaggta ctctacgtgt ttagtctgtt tttaagtgta aaataatgtt
     1621 catatctata aatttattta actttcaaac aatcctgttg aataataact aattctattg
     1681 tattatttta gttgtagaat aaaactaaat cggcaaaact agagaaattt agtgacgcaa
     1741 agctataggg actaagactt atataaatat cttatgagtt atgtcagcca gttgccaaag
     1801 agatagtgtt ctttttgttt ttatgaaagt tttttagggg gataataatt tatatgtttg
     1861 gaaataaaat ggataaatgt acacatgtat tgactgctta tattagcagt tcgtatgatt
     1921 attgtaattt tttagataca cagttagatg attttatatt agagtacgga gaaaatgtag
     1981 tagagtcttg tttacaccaa gtaatggtat tggtaagtaa gtataattag atagacattg
     2041 atttctagaa aacccttttt agttttatct acatttatat taatatatag caatgtaaaa
     2101 cgtttaaata tatttttaat attaagaata attaaaatgt taactaaaaa atataattaa
     2161 gtagacattt ctattaatct atgatacaat agtatttgtg gtaatacaca attaaatcaa
     2221 gtatatcttg atataggtta ataggagtat ttttaatgaa taatggaata gtgaaatggt
     2281 ttaataatga aaaaggattc ggttttatat caatggaagg tagagatgat gtgtttgctc
     2341 atttctcagc tatacaaact tcaggattta aatcattaga agaaggtcaa caagtaagct
     2401 ttgatatagt taaaggtgct agaggtcctc aagcagaaaa tataactata ttataatata
     2461 tagatttttc gggaaagact ctttttagag tcttttttta ttgttaatat atgtaagata
     2521 atcattggtg aagtaaataa ggtatattga gtaatgttct tgatagttta tatgctaatg
     2581 aatttcaaaa aaataagcac tcctataaaa agagtgctct taatgtaaat ataaaccttc
     2641 attggtccag cctaataccc cgtattctcc aagttctaac tattttttat atacattatc
     2701 actttcatcc atatctaaca caattatttc ttctttagaa tcaatttgta aaatatgagg
     2761 actgtgtgtt gtgacaatta tttgtgtttt taggagaatt gcttttaatg taataaaaat
     2821 tttacatacc acatagttga tataaaacta taataattac ataagattgt aataataaga
     2881 cttatcttta cataccacat agttgatata aaacaatgaa attaacatac aagagactaa
     2941 gacaattaaa atctttacat accacatagt tgatataaaa ccccaaaata aacttagcat
     3001 ctccaatgtt tacacatata caatgctctt aaaattgcag tgaacgcgca atagtgcaat
     3061 tgataatact tattatatat tctcaatgcc ttatatttca actgctaaac ctcaatttgt
     3121 cacaaatatc gctcactgca aaatttctat atttttatta tatcataaca tataattaat
     3181 attttcaatt aatttttaca tttaattatg tttaaattct ataataatgc aactttaatg
     3241 tttctttctg gaactaatat aaaaactatt tcagaaagat taggtcatgc agatataaaa
     3301 ataactatga atagctactc tcagttttta gaagagatgg acagagaagc atttgaaaat
     3361 ttgagcaaaa tactatttat tatattttaa agaagatgac ttattcaata tttgatgcta
     3421 gaatataatt aataaaaatt taattatagg tgattgtatt atgaatatta ttaaaagcat
     3481 aactttactc ttaacattat taattgtgat attaatgaca ggctgttccc caagagaaag
     3541 tgctagtgaa gttgcaaaaa aatatattga taagatgaac tctggtgact atgaaacagc
     3601 atatagttta ttaagtaaag atagtaaaaa aaaaattagt atagaaaatc ttgaagaatt
     3661 tcaaaatatt ttagttagta caaaaagaat aattagttat aaaataggta aagaaaaaat
     3721 cataaaaaaa tataaacatg atggaaaaga atataaaaat gttgttaaat tagaagaaac
     3781 ttttaatata aaaaaccttc tttataaaaa agatgattct ctaaaagtaa atagatattt
     3841 tgtagttgaa aataacaaat acaagctatt attgtatgaa agtttcaaaa aagaatttgg
     3901 tattaatagt atggaccttg ctcaacttaa gcttaatgat tttggactaa aatactttaa
     3961 agaagttgac cttatactaa aaaaagctat tgctattaat cctactgatt caaagcttta
     4021 ttatgcccaa gcatgtaatt atatgaattt tgatggattt cctggttatt cagatacaac
     4081 atataatgct ttagattcaa ttaataaatc tttaaagtat ttagataaaa atgattctga
     4141 ctacaaaaaa aatgcaagta atatatataa cttaaaagga gttattctta tgtttcacaa
     4201 aagatttgat gaagcacaac aatatttaaa taaagcctta tactttaaca aagataatga
     4261 agacccaaaa tataatctaa atacaataaa aaaacatcta aactagatat ttaatttttg
     4321 aatttataaa atattttttt atgtataatt cagcactgaa tcataatttc tactaaacta
     4381 attacaagtt taaattataa agacttattt ctatataaat attactctat aacaataagt
     4441 ttcttattta aattttaaaa tattatctaa aaatcataaa aaagaaggta taaagagcta
     4501 ccctcaagct caatatacct tataaatatc taaacttcat tttttactaa tttttctata
     4561 tctttgataa tatgttcttt agattttttt ccagctatgg catcaaaaat tgcaacagcc
     4621 tttttctctc caaacttttc tgctacatca ttaaagtcta aaaatctaac actttctttt
     4681 ggtttaccaa acatataaca cctctcatac atttttatat atttattata acttctatag
     4741 acttattttt caccaataaa cgttagacaa attagtccct ttattttttt aaactagctt
     4801 taccttcaaa attcttaaca aaaacagttt attctactct ataaataaaa atagacaatt
     4861 taatttgtag gaaactgata atagaataca gtaaataaaa ctttgtttaa tctatctgaa
     4921 aaagttatct attaaaactg ctatgaattt ataaagaata ttctagttca taaaaataca
     4981 aatatgtaaa tataatcacc ttaatattca atttatttaa aataaaaaat gtataaaaga
     5041 tttaataatg acaatatact attctcaata caatctatga tgtaaataat ttaaaactac
     5101 tatattattt aaaaatttat atttatatat gcaattctaa aaacttaata agaagtgaac
     5161 ttagaaaata aagcttaatt tcaaagactt aaatagacta ttattattag tctaaataaa
     5221 ctacgtctat caaaattata tattaaattc ttctaaaatt tttattgcaa atcttgcgtt
     5281 tggatatgta atagacccgc caccaattac acctattttt atagcttcta taatttcatc
     5341 tttacttgct ccagaattaa tacaaccctc aatatgataa caaatacact catcacatcg
     5401 gcttaaaatt gaaatagcta gtcccattaa ttccttattt tttttgctta tagccccttc
     5461 tgaataagtc ttatcatcaa gacttccaaa tactctaaaa aactcatctg tttcgcttaa
     5521 ttttttattg taaaatcccc tatattcttt catttctcta gtattcatct tctacctcct
     5581 atgtttttct tataagaata tcacaccgta cttatatttt caatattaaa tacaaatttt
     5641 cattttatgt tcattagcct aatttatatt attttttgaa atctcaataa gttatcatat
     5701 aagtaagatt ttttcagaac taaaatagaa atcttagtaa tttctttatt atctatctca
     5761 gttctgaaat atattattct tatccaaaca ttattatagt gattaattat attagttatt
     5821 tttacctgaa attttataca tcaaataaga atatataaat ggtagtatta caattacaaa
     5881 gattacaaag atattataaa aaggaatatt aataaatgca ttaataatta ataatattcc
     5941 accaataagc caaatccatc cagctaaatg gtgtgtctta ttccaattat cttcatcatt
     6001 aagagtccat ggtaaaatta ttcctactga gtaatttcgt ttacatttag gaagatagtt
     6061 tccaaataaa ataactatta ttcctaaaat taatcctgta taaaaattag actccaaact
     6121 aactcctgtt gaatataaaa ccatcattat ttgtataatt actgaaagta tagcaactcc
     6181 ccattttgat aaattttgta taacatttga aaaattagct ttcttaggct cattctcaag
     6241 tctaaaccat gtatacaaat ggatacatag cattaacaat ggtacaccaa aagctgctat
     6301 aacctttgga gcataattat ctggatttcc ataattatca aaatgaattg caacttcatt
     6361 tggtaatcta ttataaaaat atattgatac aataaatggt ataaaacata ttaaagtaga
     6421 taaaataaat tgtttattta tcgctttttt cattatcttt ttctcccttc tccataaatt
     6481 gtgaaaacca aagcatgacc tcttcgaaaa ctgaaatatt tatttcgtag aaaataaagt
     6541 tcttatactt tgtttcaaat atgagtcctg ccttttttag ttgagataga tgataagaaa
     6601 ttgtagcatt cgtcatatta aatttttcac taatttctcc tgctgacatt ttattctccc
     6661 tcaacataac caatatttct cttcttatag ggtctgaaag agctttgaat gtttctggaa
     6721 accccaacac catcacctcg ctatttagaa tttcatctaa atagaatata acataacatc
     6781 cattttattt caatagctat ttagaaattt ttctaaatag ttatttctaa atatttattg
     6841 aaaattttta tattaagatt ctcaatttac ttaagtcgct tttatttaat cttactttat
     6901 ctaatttttt taacttaaca aaagtgtatt gcaccataat tgttaaagca aaaacctaat
     6961 gattgaggta cttatatata taacaatact tttaatttta aattaaaagt agctaaaccc
     7021 aatttaaaaa gtagttaagt cctatttaaa tagaaaagaa tgaaacgaga aatatactgt
     7081 ataaaagtac ataaaaacat ctactaatta gatacaacta taacagaata tatttattat
     7141 tataataaca aaagaataaa aaaaattaat ttagcataac cttgttaaat atagatttga
     7201 atatgaattt aaagttatgt aaaattaaaa atacaggaat gaaaattcca ttcctgtata
     7261 aaatctaact tttgagtctc agtacaatct tattttgaga caatcttttt tgttttatat
     7321 tttcttatat ttaaaatata acgcatatct tatgcaacat attcatattt ttctttatat
     7381 tctttcatcg catcattcca agcgtcttgt actgttacac ctcttttatt agcaattttt
     7441 tgtgccagtt caattatctt catagctctt cttgatatgt tcataaacat tcctcctaca
     7501 aattaccata tatttcatat atataaatca aggttatatt tttatgatat ataatccttc
     7561 tattgtaaga ttacagttta tatgtgtatt atatatgaac tatattagaa gataattaga
     7621 atttttataa agctatatga ttagattaaa tataaaagct ttacaattac ttataaattt
     7681 aaaaagtcta taagctaaac taatgtttga tataccaatt ttacgaccat aaataatgcc
     7741 attactaaaa atattggttt tatcatcttt gaacctttct ttatagcaac tttagcacct
     7801 acataagctc ccattatcat cactaatgca aacactatag caattttata atctacttga
     7861 ccatttatca tgaataataa caatgaagtt atattacttg ttaagtttaa tatttttgta
     7921 tttgcagaag catgtaaaaa atcatatcca tatattttta taaatccaaa agtcaaaaaa
     7981 gttcctgtac ctggaccaaa aaaaccatca taaaaaccca ttataagagc cataagcatc
     8041 cctaatctta aatttttctt attaacagct tcaaaattat cttcttgacc taaattttta
     8101 gaaataaagg tgtaaatagc cactattaaa atcataacca caaccaaaac atttaatatc
     8161 tcttcactta ttgataatac acatctcaca cctagaacac taccaataat agtaaatggt
     8221 actaattttt ttaaaaggtt gttattaatt tttccagact ttgcatatct ataggcacta
     8281 cttatacatc ctgctgatgc tgcaaattta tttgtaccaa tggcaagatg tactggcact
     8341 ccaattgcca taagaacagg catgcttata agtcctcctc ctcctgctat agaatctaca
     8401 aaagcagcta aaaacccccc tatacataaa aaaataactg aaaacataaa attcctccat
     8461 cattctcttt caatttttct tacaatatat attatataat cttatttaat acttattatc
     8521 cagcatattc tttctatatt catttggagt tatactatta aacttcttaa atatttgtga
     8581 aaaataacta ctgttattaa aacctacttt cttacaaata tcactaatac tcatgcttct
     8641 atctaacaga tattttttag attcttctat cctaagaaga tttatatact cagttattga
     8701 aactttattt ctttcattaa aaatagaact caaataattt ggacttataa aaaaaacatc
     8761 tgatatacta ttcaaattta tattttcttt ataatatttt tttatatatt cgtttatatt
     8821 ttcaattaga tttcctttat cttttctcca aaatgctcta ttcttatcaa tacactcttt
     8881 ggaaaattca aataatacat tccataaatc ttcatcactg tttacaagtg atattttttt
     8941 attatattta tttcttaaat aagatataag ttttaaatcc tctaaatctc taagcactgt
     9001 tctagacaat acaaatatca tttcctgaat agtatctcta acatcattat atcttaattt
     9061 gtttgtatct attagattat aaacaccttc taaattcata tttttaattt ctttttcaat
     9121 ataaagttct aattcaaagg gatattcttt tgaagaatta ttgaacatat ctatcggatt
     9181 aataatatac tcttcttcta ataatctact atttgctttt ttaagtgcct cttcaagtct
     9241 tatattttct tttctactct tcaatatctc ctcatctttt atactaagtt gattttcaaa
     9301 aaaattcctc ttagctattt cctgaataaa ttcagacatc attaataaat tttcttttaa
     9361 tacatataat ctacttttag gatttaatgg aaattcattg taaattttgt ttatgtattt
     9421 tatatcaata ccagaactac ttgataattc agataaattt ttatctatat caatagactt
     9481 atatactata aaattaccac attctatata tccaactata ttttcttcta atactatagg
     9541 tatctctaaa ctggaaacac catactcaca atggtacact ttttcaaagt tatcattatt
     9601 tatgctttta acaagttttt tagtacactg ttttatattt ttacaaatat cgcaaaattt
     9661 attttctttc ttagtctcaa atatttcttt atactttaag tcatatacag atattctaag
     9721 cttcaatata ttagatattt gatttgcaag attttcaatt atgtttacat taactaactc
     9781 ctgtatattg ttttcagaaa atactatagg tatttgtctc aaactcgaaa atttatgagg
     9841 aacataagta attaatatta ctaaatcaat tttaccagtg tttataattt catgttctga
     9901 atatggtggg caataaacta tagatgtttc ctttatattg acctttttac catcaattat
     9961 ttgtattcct tctccctgaa tcacatataa aagctgttct tcacttaaat gaaaatgctt
    10021 ttcttggtag tttcccggaa aaaatcttac aattccagca ctaagtctat tgaattgtgt
    10081 ttttgatggt tcgtgtatcc acataacctc tccccaatca aatttttgta cttcattatt
    10141 tgactcacta aaatactcca taagctcacc tcttagtttt tgtctaaata ttttttcttc
    10201 ccttcttata ttaaaataat atcatcatca ttaactttca ttactataaa atcatcatac
    10261 tcttttaata ctcctgtaat tttagttgca cttagtttac tacaattcag ttttttaata
    10321 tattgataaa ttctataatt acttttttat tttgtaaaat cagtgttttc ccaaaataat
    10381 tatatctata aatgaaagta tttttactgc ttacatcaat atatgttatc ataattttcc
    10441 tttttattgc atatataaat aactgtatat aacattgtcc aatactatat agaaatacat
    10501 ctaagaaaga atattattaa attgttccaa ttagtattat ttttcttata gaaataaaaa
    10561 aactgttgga gtcttttaga aaaattattt tttacatacc ttttttgctt agatagtcta
    10621 tcttcacagc tattttgtat atacactctt ctagttcatg attttatttt aatatcttgc
    10681 ttatattgct gaaataattg aattctttta tctatcatat tgtctcttat atcacaaaca
    10741 tattgtttct tatatcacaa aaatgaaagc attcttatat cttcaatata taaacctgct
    10801 ttttttaatc ttgtaataaa tataggatac tctatattct cttcctagaa ataactaata
    10861 ccacttattt ttctattata tagaaagtaa taaatctttt tcttaataaa attcaatagt
    10921 aagtggaata aaattttata tgaataagaa cattataaaa atactttata caatagtcaa
    10981 taaaagatat tagcttcaaa ttatgttaaa gttgtattat ttaaagtgaa aaaacaaata
    11041 aaaaagaaat aatagtataa gtagaaaaat atacttaaaa caaggcgaag cctctcttgt
    11101 atctaaatca agtagagaat taaatctaaa aaagaaaata aatataaaag atgactcctg
    11161 tacattacaa gaatcatctt atggtttcat aatcactcca tggtttttaa ctgtctgata
    11221 taaaggtctc agttcactca tagcatgacc accttttccg attgctgacc ccgtcactta
    11281 tggacaaaat gtccataagt gatagtcctc tcttaaaacc ctctaaatag atatatctca
    11341 agcaccgcat tgaaaccagt tgctggaaac aaactgaagc gttctggaat agtaaaagaa
    11401 attgccacca tatccacgtt actgtcctag ttgttacttt gctaacttcg gagcgatttc
    11461 ctctaacaat acgcagtcaa attctgcctt tttgctaaga aatggcagat ggtctgcatc
    11521 tggtataaaa aaaatctgtt tgtctggcgc cactatttca tcaaaaaatt tcttcgctgg
    11581 aagatagggg ctattatagt cattttctcc catgatgtag tatacaggga cctgatagtc
    11641 aactccataa tttcttatgt cagcttcttc cactaaaaac cgcaatacat caccttgatt
    11701 gtcaatcgca tttggattga aaatggccgt attttttaac tctctcaatg aataatacgg
    11761 ggttgccagt atactgatta tattgcccaa atccatattt atagaaagcc catacttgct
    11821 ttggtatgtt cttactgtca taagagctgc caaatagctt ggaccatatt ttgctggcgg
    11881 atatggtgca atagcttcca gcgccttgat atctttttgc ttccctgcag ctttcaccat
    11941 ttcaagtgtc ttttcatagc ccacgcgctc attgtccacc atattaacgg tctgcgcaat
    12001 cccaatgtac gcgctgtaat cctccggata ggtttgcaca gccatagttc ccagaactgt
    12061 tccccagcta tgacctataa ggataatttt ctccttgttg aactcttgct tcaaatatgc
    12121 tgtcacctca tggacgtctt gcagaacacg ctccgcgctc atggtctgca aaactgctgc
    12181 cggatcgttc gcaaagtatg ttttgcctac gtttcgctga tcccagttca ccacagtaaa
    12241 atcattttcc cactcatact ggtacagatg taaaaatggc atcattgaat taccaggacc
    12301 cccatgaacc caaaggatga tggggttgtc tgcgttttgt ccccggatat ataacgcctg
    12361 ctgtatgccg ccgatctcca ccatttccat gcggtcgatt ccaggagctt ctattttatg
    12421 gcttgtacgt ataatcgtgg gggctatcag acgcaaaccg gcaaacgcca gaacaaaaac
    12481 agcaactagt atgcaaagtg tgattaatat cattttaagt accttctttc atgaattttt
    12541 ttagccatag atatctcctc taacattaaa aaaaatatgt tgctatttta gttttgttac
    12601 tctacctgtc ccattggttt cctttcataa aaaatattaa ttgattctat aatctcatct
    12661 tccaggatat caactcctat ttgtctcata agaaactgta agaattgaaa ccgtttacga
    12721 agtgtaggaa agtccccctg aaatacatct gtatcacacc aaaagttaaa gagcagcaaa
    12781 attacctctg cgcattcatc tgggaactct gtgatgatgc ttctatcctc tatgccttct
    12841 cgtaaaacat ttgctaatat tggtgctagt tttttcaaat tatcctgtgt aaatgccagt
    12901 atgatatgcg gactttctat cgcgcttgta atcatgttac tagattcttt tatcacctca
    12961 tccgttaaat gccgcctaac taaacttcta agcttctctt ttgctgtaag tccatgcatt
    13021 tcaccgagcc attgatttac tgtctctatt atctgttcaa agcgcctttc catgaccaca
    13081 ttaaaaatat cttcttttga actgaagtga tgaaaaattc cgccctttga cattcctgaa
    13141 gcatcaacaa tattttgcat acttgttttg tcatatcctt tttcagcaaa caactttgca
    13201 gaaatggtaa tgatattttc aatagcttgc tttggattgt tctttctagt cattatataa
    13261 ctccttctta ttaaaaaccg actgttggtt tttattatat aagctattcc tgcttctgtc
    13321 aagtaacatt gtgtttatgg caaaacaata aggtttggtg gttagacagg tattaattag
    13381 actaatgcga ctttccccta gatggaaccc ttggtaaatt tgcaatcttt accggctttg
    13441 attatatctg taagcaaggc aggtatggcc acacctgccc tgtttgcccc tcggatattc
    13501 attcctatcc ctcctctcct tagccattac accaaaacgc cgctcattgt ctccaatggc
    13561 ggcgttttaa agatatgatt tctcatgctg ctgaaatatg caaaaaatat aagatgccct
    13621 ttattatttt tcctacaata gaaaagaaaa gtattgaaga atgggttaat attggagcaa
    13681 atatgctttg tttggcacaa gatactacct ttatcactta tgttatcaat caattattat
    13741 ctttttataa tgagattgta ccaattcaaa aacaacatta aattataaat ctaattttat
    13801 attaaaagag actaatattt tatctatctc tattaaggat attttcttat gtatcataat
    13861 attagtctct cttattatta tatactgcta tcatcattag ttgctattta tactattttt
    13921 ataatataca aatttattca gctaacgttt tgcctaattc tatacactca tcttttgctt
    13981 cttcatttgg gtcttccatt gctgtaattc cttctccacc aataattgta gcaccagcat
    14041 tttgcatacg ttcttcccag tctctcatcc attcacaatt tccccatcca tatgaaccaa
    14101 ataatccaat ctgtttacct gataccatag attctaattc tgaaacaaat ggctccatat
    14161 acccctcttc tagttgctct gctcccatag atgggcaacc taatgcaaat ttatcttctt
    14221 cttttaaatc acttggtttc agtaagctca catctaaaac ttctggtgtc ttacctttta
    14281 acttcacacc ttcagccaca aaatttgcca ttttttcagt atttcctgtt ccactccaat
    14341 aaacaatata tattttactc atttttacct cctgtagtat tttttaatta atcaatagtt
    14401 agttatagct aactaacatc taaaaaacaa aagatttctt tatctgaaat ctctttattt
    14461 ttctaaatat agaatatcat ggttgatttt gaaattcaat acttattgat aatatttatc
    14521 atttatattt tatattcatt ttataatatc aaaacactgt atcgtatttc taatctttca
    14581 tagaaaaatt tctaacttat agtacttttg catctcttat aaatctttta tatgtataag
    14641 tacatagaaa aacacacctt ttccagatgt gctatctata atatcgtata tttatttttc
    14701 aattctaact agctaatatg tttataaatt ttatcactta atacctttat acattgatgt
    14761 aaaatcttac acctaagtaa tttttattat ttgtctcgac tctatataat atagctattt
    14821 tatcatcaaa tcaaaaatta tcttttgcta ttattcttgt atttataaag atttaaatat
    14881 aagtatccat ttccttgttc cgttctaggt attccaagcg ctctcgtaca ttttattaat
    14941 tgtgcataca gttcagcttc atctaaatct ctttcaaact cctctcttgc ccattcttta
    15001 attagtgcta atgagcctga tacgtatggt gcactcatac tggtaccact cataatagca
    15061 agtttattat ccatataagt cgatataata tttcttcctg gagctaccaa atctattgta
    15121 gtatttgaat tactaaactt ttcaaccaaa tagttctcat ttattgcacc tacttctatt
    15181 acctcagcat aactggctgg ataactatac tcacttgtac tagaatcacc atctccatta
    15241 ttacctgctg cacacactac agaaatatta ttttttactg cttgcatgac agcatttttt
    15301 aaattcttat catctttgtt tcccccaaga gacatagata taatatcaac cttgttattt
    15361 acagcaaagt taatagcatt aattatactt tgatatgttc cagtaccatc tttatttaat
    15421 gcttttgcta ttaataattt acaatctgga gctactccca taacttcatt attataatta
    15481 gatgcagcta taatacccgc cacatgagtt ccatgaccat taaaatcctc atatatattt
    15541 ttatttccat tactgtcatc actaaaattt gcaccaccaa ttatttttcc ttttaaaaga
    15601 ggatgagata tatcacaacc tgtatctatt ataccaacta caatattttt accagtataa
    15661 ccttcatccc acattcccct agcattcatt tgttttattc catatgggaa tttatttttt
    15721 gcacctctta gattttcatt tatttcatat ggaattagtt tcattctttt attcatcata
    15781 tatcctccta aaaattttat tgcttataaa aatcatgaat tttattttat aataggttta
    15841 taaatcaaat taaaacctat atctacttat ttatatttat gatatatttt ttatttttag
    15901 gagtcatatt ttattgtata taaaataata tttagataac tatattaaaa aatacatgtt
    15961 tgcttatata caaaacttac taaaatgtct ttaatatttt tgtatacaaa cattatattt
    16021 tttatataaa aagctcataa aaattttgat tatattttca taagttaaca aatgtgggta
    16081 taatatgaat ttacaaaatt cctctgtttg tataagtgct ttaccaatac aacaatatat
    16141 aatcacattg aagctatatt tgtatttata gtagataatg tatcaaaaat tggttatgaa
    16201 tagtaaaaat accagttaag catattttca tgtttctatt tttaacatta aatttccaat
    16261 cttcatatgt atatttttaa caaaggtgtt tacttattta aaaactctaa aactctttca
    16321 ttaaaatcct gtgcttcttc atatgtagcg tgtcccaatc cctcatagat aaatatttcg
    16381 ctttttttaa ttttttctac taaatgaaaa gaagcattat ttccaacaat tttatcatta
    16441 gcacctccta taattaaggt tggacatgtt attttattta attcggaaaa ggcattatgt
    16501 tctatgcaag aggtcgcttg aataataaat cgtttaaaat cctttggttt acctactttt
    16561 cctaaaaaag gaataaataa ttggtatttt tttaaatatc tttcagaata ggatttcttt
    16621 gctgtgtcaa tcattaaatc attgtaattc tgcttcttcg ccatatcaat ccaactacaa
    16681 ataacatttt ggatagtatc attttgcttt gaagaagtaa ctgccaacac aagtttctca
    16741 actaactcag gataatcaat tgccagatat tgagctatca ttccaccttg agaaacaccc
    16801 attacttctg ctttcataat tccaagtttt ttcattatat ctgcttggtc tttagccata
    16861 tcccttgtgg aatatttttc tgtaatttga ttttttctac taaatacata aaccttataa
    16921 tcttttgtaa attgcttata tctaaaagca aaagcaatcg cctgtatttt tccatgtaaa
    16981 ggaaataaac catctcctaa tcctggtaaa ataattagtg tcttcttacc actaccaaat
    17041 tcaatataat tcactgtatt acttcctatc atcatttgac aactttttgc attataaaac
    17101 atagtctatt cctcactttt cctattatca ttgtttaaat aaatctttaa atttttgaat
    17161 accatactca tcaattagag gaatatccat tttttttaac atgaataata aaaaatccaa
    17221 cctattacat acttcactgt attcacgttt aaataataca gggtcaagta aaatatcaat
    17281 actaaaaacg agcatttcag ctaattcctc tggatattca caagaaaatt ctttattttc
    17341 aacaccttgt ttaattatct tagcaatatt gggtgcaact acattgttga gattacgaac
    17401 ttcctctatt aaagcgaatg ggattttttc taaccagcta caatgtacca tttttctacg
    17461 tgtgtaatca ttcgcactat gaacaaccaa ttctattatt ttttcatttg ctgtaagggt
    17521 gtcatcagca acaagatgct ctaaaaaatt attcatttgt gagtaaagtt cttttgtgac
    17581 acttctcagt atatcttctt tagttttaaa gtgatgatac attgcacctc tagatagacc
    17641 agataaattg ataatatctt gtgtagatgt tgaatcatat cctttagacg aaaatagtga
    17701 aaatgctgat tggataattt tttctttact atttttactt ttttcttctc tattcatatt
    17761 tccctcctaa aaacagactg tctgtttgtt ttttattata cattttaata tttattatgt
    17821 caagaattaa cttactttca atttcttcat agttcagaag gaacgaaaag cattccacat
    17881 caaactttta aaaagttgta ttacctcaac aaggtctaaa agatacatct ctatgctgta
    17941 ctcgtattcc ttataataga gatgtattgt tttcaaagat aatcaagatt ttgctatgtt
    18001 tttcatacaa aataaaaagg atacctcatt agtgctataa cacctaagat atccaattaa
    18061 tattttctta actttttcta atttacataa atatagtcta ctattttaaa actgctcctg
    18121 tatttgctga tgttaccagt ttagcatatc tacttaagta tccatgcttt attttcggtt
    18181 ctaaaggttt aaattttaat tttctatttt ctatttcaac ttcatctact tttaactcta
    18241 attttttgtc tggtatgttt atagaaatta tatctccttc ttcaactagt ccgattaaac
    18301 caccttccat agcttctgga gaaatatggc ctatggatgc ccctctagtt gcccctgaaa
    18361 aacgaccatc agtaagaagt gctacatgct tatcaagtcc cattcctgca actgctgatg
    18421 taggagatag catttctttc attcctggac caccctttgg accttcatat cttataacta
    18481 taacatcacc tttgtttatt tttttaccaa aaatagcatt tacagcttct tcttctgaat
    18541 taaaaactct tgctggtcct tcatgaacta acatttcttc tgcaactgct gactttttta
    18601 caacagctcc atttagagca agatttcctt tcaatattgc aagcccacct tggttactat
    18661 atggattctt taatgtatgt attacttctt cattttctat ttcacaattt cttatatttt
    18721 cccctatagt cttgcctgtt acggttttgc aatctaaatt tattccattt atctttgaaa
    18781 gttcgttcat tatagctggt attcctcctg ccatatgtaa atcttcaata tgatgttttc
    18841 cacttggact taattttgtt aaacaaggag ttttttcact tatttcatca aaaaaatcta
    18901 agttaagctc tattcctgat tcataagcta ttgcaggtaa gtgaagtact gtatttgtag
    18961 aaccagccat tgccatatcc acagctatag catttttaaa agcatctatt gttaaaatat
    19021 ctctaggttt tatgtcgttc ttaagtaact ccataacata catacctgca tattttgcta
    19081 tcctttttct ttctccagaa tgtgacgcag cagtaccatt atatggtata cccatgccta
    19141 aagcttctgt taaacaattc atgctatttg ctgtgaaaag accagaacat gaaccacatc
    19201 caggacaaga gttcatggct aactcttcta attcttcttc acttactgtt ccatctttac
    19261 atgctccaac accctccatt gcagagttaa aatcatatac ttttccattt tttttacctg
    19321 gaagcatagg ccctccactc acaacaatac ttggtatatt aagtcttgca gcagccatta
    19381 acatacctgg cactatcttg tcacaattag gtataagtac caaagcatca aaaccatgtg
    19441 ccatagccat agcttctatt gaatctgcta ttaattctct tgaagccagg gaataattca
    19501 ttccgacatg ccccatagct ataccatcac atacccctat agcaggaaat tctaatggca
    19561 aaccaccact cattcttact ccattttttg cagcttctgc tatttcatca agatgcagat
    19621 gaccaggaat cacctcattt tgtgcattta ctatacctat taatggtctt tcaatttctt
    19681 ctttagtaag acccattcca tacattaacg ctctctttgg agctccttct attccttttt
    19741 ttatatcact tctcataata ttacctcttt cttacttttt taaattttca taatattatt
    19801 ataatatatt tttctattaa ttaattacga aaattgtaaa aaaatattag catatactaa
    19861 tgaaaattta ttacgatttt ttatcaatat cttacacaat cgtattatat aatattatct
    19921 tttcaatata ttttataaac taatatattt tacaaattgt tgagtaatat tttatgatat
    19981 aaatcagaag aggcacttct ataatagaaa gtgctcaact atgaataaga ttaataatat
    20041 atttgcctta gatatataga cttttataaa tattaaagat taatcaaatc tgatgtttga
    20101 aactttatcc caattaatct tccttgaata gtcttctgga tgtgataatc taactatatg
    20161 agttgaatta tcatagctgt aataaattgt agtagtacca tcaactggat tctttttgta
    20221 tccttgatac acagcactat ttggtacttt cttttcaaca aagctatatg cagatgaagt
    20281 atcaatagtt tctattttat taggaaatat aaacatataa aaagctacta ctataaaaat
    20341 acatatgcat gtaatactat accatttaat ccatttcata aaattcctcc tactttacca
    20401 aaatatacta ttattatttc catatattta taatatttat gcatacgata aaatttttat
    20461 atccctaact atataattgt agaaactttg attatatata ttttttaatc ttaataatat
    20521 atttttataa attaataaat ttttaagatt ttattaaaag atttttaaaa cttacagttt
    20581 aatataaaga tagtacttat aaaactaaaa acataaattt gaaaggggac aaaataaaat
    20641 gatatggaca ttttattcat ttatttgcgt tatattacca tgcttaatat accaaatagt
    20701 cgttataaaa cgaaaaaatt tgaaagttga aaagaatgta atgatacatt tagtatgggt
    20761 atatattttt ttattatata tctatctagc acttagtaaa gctggaattg gtagcatatg
    20821 ggatatagga aggtatagtg ggctttttag aattgatgaa ataaatctaa taccttttga
    20881 ctcagttggc atcttaacat atattttaaa tataattatg tttatgcctt taggattttt
    20941 actaccttta atttggaaaa attttagaaa tattatcaat gtttcattaa ctggattagg
    21001 tttttcatta gcaattgaac tttgtcaatt gtttaactta agagctacag atattgatga
    21061 tttaatgatg aacacattag gtgctgtttt aggatatttt atatgggtag gtgtaaataa
    21121 tttatttaat cttataaaga aaaaagatat tttcccatct atatataaaa atattattca
    21181 agatgagatt gctattacaa ttgatgatga cataagcaaa aaaattacct ctctttacaa
    21241 aaatgaagct attatatatt taatacttgc tgttttagga gaatttttat tgtttaattg
    21301 gtccattata ccttattaaa taatttattg ataaaataag tgtacttcga tatttacaaa
    21361 tatgtaaagc cctctttcat actacaaaag aaagtgggct ttagttatta aattaaaatg
    21421 cacactcact atttagctgg catctgaaat attttattaa cattgtatct gactttattt
    21481 gatgttatat aatcatattt tgttccattt tcaatgaagt tttgtatatt gtccaacact
    21541 tgtatagctc ttgtattatt ttcataatct cctattttta cagtatcaat tttactttca
    21601 aatactgcat aaacttgact accatctatt ttaatttggt ttatctgcat taaattagtt
    21661 ttatcttgac ttctaattat tatcatatca attcctccat ttacttaatt cattttaatt
    21721 t
//
LOCUS       DAMJYX010000061        21686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJYX010000061 DAMJYX010000000
VERSION     DAMJYX010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.061"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            220..1818
                     /locus_tag="PC479_002365"
     CDS             220..1818
                     /locus_tag="PC479_002365"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2804905.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2057..4102)
                     /gene="bclA1"
                     /locus_tag="PC479_002366"
     CDS             complement(2057..4102)
                     /gene="bclA1"
                     /locus_tag="PC479_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2804906.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGE
                     IGPTGATGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGAT
                     GSIGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIG
                     NSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTT
                     EPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
     gene            complement(4780..5304)
                     /locus_tag="PC479_002367"
     CDS             complement(4780..5304)
                     /locus_tag="PC479_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2804907.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(5369..6121)
                     /locus_tag="PC479_002368"
     CDS             complement(5369..6121)
                     /locus_tag="PC479_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2804908.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(6152..7450)
                     /locus_tag="PC479_002369"
     CDS             complement(6152..7450)
                     /locus_tag="PC479_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2804909.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(7467..9731)
                     /locus_tag="PC479_002370"
     CDS             complement(7467..9731)
                     /locus_tag="PC479_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2804910.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(9986..10804)
                     /locus_tag="PC479_002371"
     CDS             complement(9986..10804)
                     /locus_tag="PC479_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2804911.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(10797..11495)
                     /gene="cbiQ"
                     /locus_tag="PC479_002372"
     CDS             complement(10797..11495)
                     /gene="cbiQ"
                     /locus_tag="PC479_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2804912.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(11498..11782)
                     /locus_tag="PC479_002373"
     CDS             complement(11498..11782)
                     /locus_tag="PC479_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2804913.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(11772..12524)
                     /locus_tag="PC479_002374"
     CDS             complement(11772..12524)
                     /locus_tag="PC479_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2804914.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(12706..12898)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(13030..14802)
                     /locus_tag="PC479_002375"
     CDS             complement(13030..14802)
                     /locus_tag="PC479_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2804915.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(14816..15745)
                     /locus_tag="PC479_002376"
     CDS             complement(14816..15745)
                     /locus_tag="PC479_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2804916.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            15966..16151
                     /locus_tag="PC479_002377"
     CDS             15966..16151
                     /locus_tag="PC479_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2804917.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            16144..16845
                     /locus_tag="PC479_002378"
     CDS             16144..16845
                     /locus_tag="PC479_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804918.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            16847..17770
                     /locus_tag="PC479_002379"
     CDS             16847..17770
                     /locus_tag="PC479_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804919.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            17927..18850
                     /locus_tag="PC479_002380"
     CDS             17927..18850
                     /locus_tag="PC479_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804920.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            18843..19580
                     /locus_tag="PC479_002381"
     CDS             18843..19580
                     /locus_tag="PC479_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804921.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            19591..20364
                     /locus_tag="PC479_002382"
     CDS             19591..20364
                     /locus_tag="PC479_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804922.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(20905..21513)
                     /locus_tag="PC479_002383"
     CDS             complement(20905..21513)
                     /locus_tag="PC479_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2804923.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         6917 a         3752 c         2831 g         8186 t
ORIGIN      
        1 tttattttaa ataaaagtaa atttaattcg atatttgatt gggtaaaagt atatataata
       61 ataattttta tattaaattt gtagtattta tgtaagaaat aaattttata taatattttt
      121 tcactaaatt tttttctttt tacaacaaat actcactacc ttttgatata atatttttat
      181 caattattat tatattcaag ttttgaaagg agtattaaaa tgagtttatt agttttcgga
      241 cataaaaatc cagatacaga ttcaatatgt tctgcaattt cattagcaca tttaaagaac
      301 aaactaggaa taaaagcaac agcttatgct ttaggagata tcagaaaaga agctaaatat
      361 gccttggatt actttaaggt agatgcacct gaaattttag ataatgttag aatacaagtt
      421 agagatttaa attatgataa agtagtacct ttaacaccaa cttcatctat attagaggct
      481 tataacctta tggatgaaaa aaatgttaaa acactacctg taacttatga tgatggaact
      541 tttgctggta tcattactat gaaagaaata gctaaaaatc tacttcatca acactttaca
      601 actatacaca catcactatc aaacatttgt aaaaacctta atggtactat tcttgtggac
      661 tgtggtgaag atataaaagg tagagtagtc actttatcat ttggaatgga gactctaata
      721 gagactttaa aagaaggaga catagctatt gttggagata gatatgatag tattgaatat
      781 gctattgata caaaagttaa acttttaata cttactaatc atacagagat ttcaaaagat
      841 ttgctagctc ttgctaagat aaatggagtg tctgttgttt ctgttgaaag tgacaattat
      901 aaaacttcta atgtaataaa tcaatgtagt ttcttagact ctatagaagc tacagaaaat
      961 ttaattacat tcaaagaaga tgattatatg gatgaaataa aagagattat gctagaaaca
     1021 aacttcagat cttatccaat attagatgat gataataagt ttttaggttt agtttcaaaa
     1081 ggtcatctat taaacccatc taaaaagaat gttgttttag ttgaccataa cgaatatgct
     1141 caaagtgctg atggaattga acaagcaaac atagttgaaa taattgacca tcataaactt
     1201 ggtggtattt ctactgatgt tcctatgtca tttagagtta tgcctgttgg atgcaatagt
     1261 actataattt atcaaatgta caaagaaaat aatgttgaga taccttatga aatagccggg
     1321 cttcttttat ctgctatatt atcagataca ttattattta agtctccaac aacaactgat
     1381 atagataaga aagcttgtga agaattaagt aaaatagcaa aagtagacat ggaaaaatac
     1441 gctatggata tgtttaaatg cggtacttct ttagatgaat atactataga agaaatagta
     1501 aatatggact ttaaagaatt taatatgagt ggacatagag ttggtattgg acaagtattt
     1561 accttagaca tagactctat atttgctaaa aaagatgagt ttttatctta tataaattcg
     1621 actgattatg acaaactaat tcttgcggtt acagatataa tcaaagaagg ttcttattta
     1681 atctataaag cagaagacag cttaattgct aacgcatttg gtgttgaagg tgttcaaggt
     1741 acatttgcac caggtgttgt atcaagaaaa aaacaattag tccctaattt aactactgca
     1801 atcaaaaatt ttaaataata taaaattttt aaataacgaa aactgtctca aatctatatt
     1861 tgagacagtt ttttattata tatatatata ttgataataa agttaaataa aaatccataa
     1921 atttaaaatt tttatgaata tattttatta ttattgttat tttattcatt ctaaaatagg
     1981 taaaactcaa tatataaaaa tacttttgtc atttaaataa agcctaaata gtttttaaga
     2041 ttattttaga cacgttttaa gtcataacag tatcagctat tctaaatatc gtcaaagtag
     2101 ccgcagttcc tgctgattgc ccatttcgta tccctactgt tgtagatgaa aaattattta
     2161 ttgtaaaagt agtcccacca aataaaaaac cagcaaaata tccagaaagt actcctccgc
     2221 ctcctggctc agtagtaagc cctgccaaat cttttcctcc taaaattggg aataggatag
     2281 cgaaagaata ttgatttata cttgatgaac ctggttgtaa tgctactgaa actgatatat
     2341 aatacacacc attttcttgt actgtaaacg taccatttgc atctactgtt atacttgaac
     2401 tatttcctat tattgtttgc caacctgtta tagcatctcc attatttagt gtttgtggag
     2461 atggtgcatt tacgaggaag ttagcaactg gctgagagga attgcctgtt gctcctgttg
     2521 gacctgttgg gcctgttact cctgtcgctc ctgttgctcc cgttggtcct atacttcctg
     2581 tcgctcctgt tggacctgtt actcccgttg gccctatact tcctgtcgct cctgtatttc
     2641 ctgttggccc tatttctcct gtcactcctg ttgggcctgt tgctcccgtt ggtcctattt
     2701 ctcctgtcac tcctgtattt cctgtcggac ccgttgctcc cgttggtcct atttctcctg
     2761 ttactcctgt atttcctgtt ggacctgttg ctcccgttgg tcctatttct cctgtcgctc
     2821 ctgttgggcc tgttgctccc gttggtccta tacttcctgt cactcctgtt gggcctgttg
     2881 ctcctgttgg tcctatttct cctgttggtc ctatttctcc tgtcgctccc gttggtccta
     2941 tacttcctgt cactcctgtt gggcctgttg ctcccgttgg tcctatttct cctgttggcc
     3001 ctatttctcc tgtcactcct gtcgctcctg ttggacctgt tgctcctgtt ggtcctatac
     3061 ttcctgtcac tcctgtcgct cctgttgctc ccgttggtcc tatttctcct gttggtcctg
     3121 ttgctcctgt tggtcctata cttcctgtca ctcctgttgg acctgttgct cccgttggtc
     3181 ctatttctcc tgtatttcct gttgggcctg ttactcctgt tggtcctata cttcctgtca
     3241 ctcccgtatt tcctgtcggg cctgttgctc ccgttggtcc tatacttcct gtcactcccg
     3301 tatttcctgt cggacctgtt actcctgttg gtcctatact tcctgtcgct cctgtacttc
     3361 cggttgggcc tgttactccc gttggcccta tacttcctgt tgctcctgta tttccggttg
     3421 ggcctgttgg tcctatgctt cctgtcgctc ctgtacttcc ggttgggcct gttactcccg
     3481 ttggtcctgt acttcctgtc gctcctcttg gtcccattgg acctgtaatt ctaattggaa
     3541 gacactcaca tttaggataa caattatcta taattctatc ttcaaatgtt acaagtatat
     3601 acggtttgtt acaacctcta ctagatgtaa attcaagaat ctgataaggg taatttaaag
     3661 ataaagctaa cccataatta ggatatttat tcattgccca tgcttttata gttcccgtaa
     3721 tatttaaaca aatatagttg gatgtatcac ttatcccaac tggtaaatat cccctaatat
     3781 tttccataga cactctatca ttccatgtta tagttcgtaa attataatat tcacttattc
     3841 ttttaatttc aatatctact ttttctttaa aaatatgatt cttatttgaa tcaatatgaa
     3901 taaataactc tgcttttaat atacttttgt cttttaatat atctggtatt ctcactttaa
     3961 aaaaagtaat caacttttct tttacaaaac tatttgaaca tttacttcct attaagcaat
     4021 aatctaaatt actaaagttt ttatctggat attttttaga tataaaagta tcatcattta
     4081 aataaagtat aatatttctc atttataatc cctccatttt gggtctatat catactatgt
     4141 ttaaaataca tgcttagtta caatataatt aaaatttata gtattaaatt tttatataat
     4201 aatgctgatt tctacaaaaa aagaacatgt cattagaaat ttctaatagc atgttctttt
     4261 tcattattat atattacact ttaaattatt ccttgactgg cttagctggt gtaaactaaa
     4321 ccagtctttc taaaatatta agcacttttt tcttattatg ttttttagga tttattgtct
     4381 atattattgt aaaccaaagt tataaaattg taaatagaat atattaccat aacaatatct
     4441 ttttataagt aaaaattata aaaaacctta tttttattaa atagaataaa taaaataagt
     4501 ttaaaaaata ctgaaataag tgttttccac atattttata tatttattta atacattatt
     4561 atttaaaaat aaatatataa acaatcataa taaaattttt ttaataaaac tttatttttt
     4621 agttaaaatc atacaatata tagatttcca caatacaaat aaattaaaaa gtgaaatgta
     4681 tttgtctaaa aacataataa catttcactt tatcctcatt tttaagtaat atatattttt
     4741 aatttttatt tataactaaa tctagttttt atataaattt tagttttttt ctacatctga
     4801 gtaatctatt ccaaaagttt ctacagtaac agatttcata acttgtggag tttgtggctt
     4861 atcagctgaa tctgttctaa ctgatgctat ttcatcaact gtatccatac cttctatcac
     4921 tctaccaaaa cctgcatact gaccatctaa gtgtggtgag tttttatgca ttatgaaaaa
     4981 ttgagaacca gctgaattag gtgccatagt tctagccatt gatatgactc ctctttcatg
     5041 ttttaaattg ttagtaaatc cattaccact aaattcacct tttattgagt gacctggacc
     5101 acccattcct gtaccatttg ggcatcctcc ttgtatcata aatcctggta ttactctatg
     5161 aaatattatt ccattgtaat atccttcatt tactaatgat acaaagtttt taactgtatt
     5221 tggagctata tttggatata actcaatttt gatttctttt ccattttcca tttctatagt
     5281 tactatagga tttttatttt ccataattta ttaccttcct tttcattagt taaattacat
     5341 aattacattt attatattat actctaaatt aaagtctatc aagtatattt tttcctattt
     5401 catatccttc ttgataaata gttttacaaa actcatcatc aaagttaagc gtgtctttag
     5461 gagcaaattg agtattaggt ctaactacta ttattttatc ttcatcatat acttttttaa
     5521 tatcttctgg caactcataa ttatattttg tggatattaa aataatttta tccacattat
     5581 ccccaattaa tggttcaatt aatgcactcc taattaaacc tccatctaat ttgaaaccat
     5641 cataaattcc ttcttgcaca tctttcacaa attgttcttt aaatccaaga gttgcgtttg
     5701 gatttgatgg taacaaccca ctgtatgaaa tgctgataat ggcttcatta cgaggcattt
     5761 ttgatacgtc aacaatcttt tctgatacta ctttattatc aacttcaaca taattaacat
     5821 aaaagttcat ttcttctata ttactatcat ctagttccct taggttgtct atagcaggag
     5881 aattatccac agtattattt actattttta catttccatt ctggatatct tttatatttg
     5941 tccacatttt ctccaagtta tcaacattcc ctgtgtacaa ataatatcca tttattgctc
     6001 ctattgatgt ccctgaaatc gaataaaatt tatttattcc tccatcatac aaggctttaa
     6061 ctactcctgc ttgataagcc ccttttgctc ctccaccttc taaacataat cctaatttca
     6121 tagtctcatc tccaaattgt ttatcttata tttatatttc aaaaaactta ctaaatgttt
     6181 tatatatact atagtggtcc tcaacatttc ctaattctct tatagaatgc attgctaaaa
     6241 ttggacttcc cacatccaca gatggtatat ctatatgagt agaagatatt ggacctatag
     6301 tacttcctcc tctttcatct gacctattaa caaattcttg atactcaaca ttagcttctc
     6361 tacatatatt tttataaata gctatagaat atccatcact agtatatgcc tgactagcat
     6421 ttatctttat aaccggacct tttcccatta ttggtttatt agttgggtca tgcttttctg
     6481 gaatgtttgg atgaatagca tgagctaaat ctgatgatat cataaaagat gaataaatag
     6541 actcaaagaa tccctctcta tccttaccca ttgatatgca aattctctcc aatatattta
     6601 acaacatgtt tgaatctgca ccttgtttag tagagctacc aacttcttca ttgtcaaata
     6661 cagccattac atttacacca tgttgacctt tagtattaac aagagcattc aaactagcat
     6721 gtaccattgc aagattatct tgtctcccaa tagatataaa ttcatcatta gcccctatta
     6781 aacttccttt ttcaaattca tataaaaata aatcaaaatc tataatatcg tccatattta
     6841 catctaagtt agatgctatt tgctttataa gaaaattatc tttttctaat gtatcattta
     6901 acatcccaac taaaggtaac atatcttttt gcttattata tgagtgacca tcatttatac
     6961 ttctatttaa atgtattgct agatttggta ttatacaaat tggcttttta aagtctataa
     7021 gcctttcatt tggttttaat atactatcac cttttaaaac tactctacca gctatcccaa
     7081 gaggtctatc caaccatgta cttaatattg ctcctccata gcattctgta tttaacttca
     7141 aataagattt ttcagcttct atttctgcat ttggtttaat actaaatgta ggagaatctg
     7201 aatgagagcc tattattcta aatccatctc tttctatacc ttctgaattt actacaaaag
     7261 ctactatagc tgataaattc ttagtaacat aatactttcc accaactctt agattccatc
     7321 tttgattcaa aactaattct tcaaatccat ttttatctaa gatttcctta gtactctcga
     7381 ctgcatgaaa agacgacgga ctatcatata tgaagtctat taagttttta gcaaagttta
     7441 tactatccat tcctcattta cctccattat aaaatattta gtgttgtata ttctttattt
     7501 atttttttaa ttccctgttg gtcaaaagct atagtaacat ctgtaccaat tatagaaacc
     7561 actgtcccaa ccccaaattt aggatgatgt accttagaac caagttttat atcatctgga
     7621 ttagtttctt tattagaatc ttttattgta gcattaactt tatctgcaac tttagatttg
     7681 cttatagtac tcatatactt tttagtatat ttatctaaca tattataact tgctttagaa
     7741 tagcttagtt ctttttttac tttgtacaat ctttctatac attcttctgg cagttcttcc
     7801 ataaatcttg aagctattgc aacatttgtt ttaccatata atgttctctt ctctgtcaat
     7861 gacatatata gctcttcttt agctctagtt ataccaacat aacaaagtct tctctcttct
     7921 tctatttgtg aatcactcat agattttata gctcttgata taggaaataa cccttcttcc
     7981 atacctgtta aaaacacaac agggaactct aaacctttgg atgtatgaat tgtcataagc
     8041 gaaaccctat cattgtcttc ttcatcattt gattcagatg taagtgctat acttgttagg
     8101 aatgtctcta agtctttatt ttcataagta tcatcattac tactttcaaa ctccaaagct
     8161 atagaaataa attctctcaa attatcaatt ctgtcttgag cttcttcacc tttgcctgtt
     8221 aaatcttttt catttttatt tcttatctct acaagctcgt ccatatatcc tgtagtatcc
     8281 aagacttttt ctataagttt gctaacagga taaacttctt ttatagttct aagagtccct
     8341 ataatatcaa caaatccact tatacttgcc cttgccttag tagatatatc tgaatttgtt
     8401 tctatatcaa ttaatactga gtatatactt tcctgtttta agttagctct atcttcaatt
     8461 ttttctatag ttctaagccc tatacctctt ctaggaacat ttataatcct ttttatagat
     8521 atatcatctt gtggattttg aataactctt aaatacgcca acaaatcctt tatttctttt
     8581 ctctcataga attttgtacc accatatata ttgtatggaa tttgacttct atttaaagcg
     8641 tcctctacag gtcttgcctg tgcatttgct ctataaagta ctgcaaaatc cttataaggt
     8701 ctattttctt ttctagctat ttgtgcaatt gaatcagcaa tgaaatcagc ctcttctatt
     8761 tcactaccag ttaattgtat ctttataagt tctccttctt ttttttcact ccaaagcttt
     8821 tttctttttc tttctatatt attagatata actttattag ctgcatctaa tataacttgt
     8881 gtagacctat aattttgttc caactttaca acatgtacat tgtcataatc tttctcaaat
     8941 tcaagtatgt ttcttatatc tgcacctctc catccataaa tactttggtc atcatctcca
     9001 acaacacaaa tattttgatg ttctcttgct aataatttta tcaattcata ttgagcttta
     9061 cttgtatctt ggtactcatc taccattatg tatctaaatc tacttctgta atatgctaac
     9121 acttcatcat tagccttgaa aagttctaca gttttaagta ttaaatcatc aaagtcaagt
     9181 gcactatttc tttttaaacg gtcttgatat agcgcataaa tatctgcaat cttaaccatt
     9241 ctattatctg ccatattgat atctttaaac tgctttggag tataaagttt gtcttttgct
     9301 cctgaaattg cacttataac tgcttttggt tcaaacactt tatcacttaa atttaattct
     9361 tttagacagt ctttaaccaa tgtaacttgg tctgctgaat cataaattac aaaacttcta
     9421 ttgtaaccaa tcttatttat atcttttctt agtatacgaa cacagcaaga atggaatgta
     9481 cttatccaca tatccttagt ttcaggacct atattttgtt caactctctc tctcatttca
     9541 tttgcagctt tgtttgtaaa tgttatagct aatatatttg gtgcttgtac acctttatct
     9601 tctataagat atgctattct tgttgttaat accttagttt tacctgaacc agcaccagcc
     9661 agtataagta ctggaccttc agtcttttct actgcttctc tttgagcagg atttaatgta
     9721 tctaaattca tatttctcct cctgttaaca gccataaata tattttaatc taaaatatga
     9781 attacttcaa attataatta tgtattatat taatagatta tttccaatat attacaatta
     9841 aataacattt ttccatataa tttatttcat tgtttaataa aaaaaataac cttatgattt
     9901 ggggaatcat aaggcacctt taaggggtct aggttaaggg ttattatata taaatctatt
     9961 tttttaaaat aggtttaggg cttaattatt tatttgtatt aagttctctt tccttccaat
    10021 acttatacaa atcttcttct ttcttaaata agggtaaatt catatttcta tgtactttta
    10081 ctaaccatgg ggattctagg ccagcttgta taatattatt ttcatttata aacacatttt
    10141 ctgcttttcc ttcatcaatc aaaatcccct tatccaatac atatatgtaa tcacatattt
    10201 catacattaa attcatatca tgactggata ttactatttt tatattattt ttatttaatc
    10261 cctttattat atctacaata gacctagtac ttactgggtc taaaccagct gtaggttcat
    10321 caagtaatac aatttcattt tccattgcta taactgatgc tattgccact ctttttttct
    10381 gtccatagct aagaaaatgt actggcctgt ctataaaatc tattccatta acagctacta
    10441 gagctttgtt tattctctct ttaattgttt tttcatccat accaatgttt ctcattgcaa
    10501 aggctatatc atcatatact tttgaataaa aaatttgttt ttctgggtct tggaagacaa
    10561 tacctacatt ttttctaagg ttgtataaac ttctcttatc atattttaac ttttcttctt
    10621 taaaaagaat ttctccagat gtaggcttta atatacccat taaattcata aataatgttg
    10681 attttcctga accatttgaa cctattatac ctataacatt tccctttgaa aaatccatat
    10741 taatgtttaa tagtgcattg gtattttttt catattgata cgttaaatta tttattttaa
    10801 acatcattat cccctactat gtgaaattta ccatcataca attttatgtc taatgatata
    10861 cacatatcat cataccttgt cataagtctt ttgtacagca tacttccaag aatacctaag
    10921 gatttatatg aagtttttaa atttatatat ccaaatctta gctcttgtga cttgtatatt
    10981 tctgaaaatt cttctaaaaa tatgaatata aacctatata ttaacattga taactcgaca
    11041 aaagtgtcag gtaaatgaag ttttttaaaa aagaatatta attgattaaa aggtgttgta
    11101 agtatacaaa aatacacaca agtcaaacaa gacatcgctc taaataacac atgaatagac
    11161 atgttaatag acgcctttga tattcctaca ttaaaactaa aaaattcaaa actatataat
    11221 aaatctgctt tattaaaaga tatatttaca agagttattc ctatactaag aaataaaaag
    11281 tacattggta ttctaagtaa ttttaaataa ttttttaaat ctattccagc cacacaaaca
    11341 accaaaatac tcattaaaat cataattcca cttaaaataa atatattttg tattaccata
    11401 gaagcaatca gaaaaattac tcctatggct acttttttgt ttggatttac tttactcaat
    11461 ctatttgtat atgcatattt atcaattata agcatgatta acttctcttt cctttttgaa
    11521 gacctaatat gtatcctatt acaccagccc ctaaagctgc ttgtgcagag aataataaac
    11581 tttctatttc tccacttggt ggctcatatg gactgctaaa ccatggtttg taatttggat
    11641 ttatcttagt aatttcactt tcagcttgac catctgctcc tccatattca gcacctgagt
    11701 tgacaagtaa tgggaatata ataagagcta ctactaataa aagtaataat atgttagttt
    11761 ttgtttttgt tttagcactc attagcttta actcctttat cattattttc tagtatgctt
    11821 ttttctccgt tttctgtaat taaattatat acaattacag taagtatacc ttctgctatt
    11881 gctattggta tttgagtcat aaagaaaatt cctaaaaatt taattgctga agccattacc
    11941 ccacctgatg ggtctgggaa agccagtgct aattgtattg aagttattgt atatgtggct
    12001 aagtctccta ttgctgctgc aagaaatact ggaatagcat tatttccatc ttttttcttt
    12061 aaagccttaa atattaggaa tgatacgatt ggccctatta tcgccattga aaaagcatta
    12121 gctcctaaag tagttattcc tccatgtgct aataacaatg cttggaatat taaaactata
    12181 gttccaagaa caaacattac gcttggtcca aacattattg ctccaagacc tacaccagta
    12241 ggatgtgaac aacttcctgt tactgaaggt atttttagtg ctgataatac aaacacaaat
    12301 gctccacata aagctaataa tactttcttt ttagggtctt gctttactat tttacctatt
    12361 gattttaatc cgaataagaa aaatggtata aatataactc cccatgctat agaccatttt
    12421 actggtaagt aaccttccat tatatgcatt gcatttgatg taactggagt taaaatgatt
    12481 aacataagtg cagctataac ccctaattta atattctgtt tcatcttagt atccctctct
    12541 ccctttctaa taattaaaac ataataatac atgtctgatt tatttaataa aataaaaaag
    12601 cctaactcga tggttaggct tctaattttc cacaatctaa atacaaacac ataaaacaaa
    12661 catatctgta taattattat aagaaacttt ctccatcccc gaagaaatta ctttaatatt
    12721 ttaggcaggt ctcctgactt agcttcatcc tactcctttt ccttcccatg agatataaaa
    12781 aatatcatca tagtggctaa caaaagtttc gtctgcatca cagtagcggg ggctgtaaag
    12841 gaatttaacc tttttcccta ttaatcttgc ttaaagaacc tataatatta tatatttaat
    12901 ttttaaatta ttcttaaatc attttctata taaatcattg tatacctttt ctgtgaaaat
    12961 ttctacattt tttataaatt ttattcattt ttatattttt atgtatataa attctttttt
    13021 gtaagtttat taatttctaa ttatcttctt taaatacatt gaaactacat cagaaaatct
    13081 agttatatct cttgaatata taaaatcatt tccatatatt aatctttcag cttcaagttc
    13141 tgattctttt cctgtaaata ctcctaatac taatatattt tgctttctag cttttctcac
    13201 ttcattggct gtatctctca aaccaactat acccctataa gccttttctc ctcttaatgt
    13261 tctctcacta ctttttccta tctttacatc atttggtctt ccatcgctga gaactatcag
    13321 gattttattt tcttcatctc tatttattag accactacat atagatttta ttgccaaacc
    13381 atctctatta ttaccagcac aaaaatattc aaatatattt tcactatttt ttatactatc
    13441 atcataatct ttaaatcttt ttaatattgt atagtctaaa aaactagaaa atcccatcac
    13501 tctatttggt atgccaactt ctgttagagc atttgcaatt atgtaagctt gaattgccac
    13561 atttccctga tttctacttt gagagccact tgcatccaat aatatatcaa taacgtattt
    13621 ccctttttca ttttctatat ctttataaaa tattttatta ttattacttc taccaactct
    13681 ccaagctcta ttggctaata tagtaccaca atcagaatat actctacttt tagcattttc
    13741 ttctattaaa attcttgaca gactttcttt taatctcttt atttgctgtc tatacattct
    13801 agcatgttct ctaaattttc ctaaattatt ttctttttgt cttgttacat attttagttg
    13861 aaatacatta ttacactcac ttctaagaac tccatctgta aagtgtattc tacaaccttc
    13921 atgaacattt ctacaatttt gtgtctcaat ttttttaatt tcacctttac tcaaataagt
    13981 ttttccataa taatgttcta tttttgaata tatcttattt gcagtgtctt catcaacata
    14041 tataactcgg tctgctgaat tgctacattt agaatcatca aaatccccta caccttctac
    14101 aagcatggat gaagatattg aatctatatc actttcaata atattttctt cttcatcact
    14161 ataaagttct tcatacatga aatcagcaaa tgtatcaaaa tttacttcta cactttctac
    14221 attattttca tcaataagac tattttcttc tccatttaat atgtattcaa aataagttaa
    14281 atatataaca tctaatgctt ttaaaatctc cataatatct tgattggtat ctatatttct
    14341 tatatcatta actattcttc tagtcatact atctaatgct gggtgcttgt ccatactata
    14401 aagtacaaat gtgtatttaa ttttatccat aatattatca ttgtgtattt tggtaaaacg
    14461 tgaaacaata tctttataag cattccttct tatgtcttct atccctggcc tgtctttaat
    14521 gaccttttct tcaactattg tatttaatat aacttgaatt agacctagta taacatcttt
    14581 gttgtatcct tttcttattc tatttgtaag atatttttta actatatccc aatctacata
    14641 ctttcttcta gcacctgcaa ttattgcgaa atatagacct atttcttttg aagagtaatc
    14701 tttttctgat atatctatac tttcattgta atcaccagct attgtccatg ataggttatt
    14761 agctctattt tcaaattcat aatcatcata tatccatttc ataatctcac ctatattaaa
    14821 tatgacttat tggaaacaca tctatactct cccatttatt aggtattcta gttttaatta
    14881 catctccaac catttctttc tcatatacat caaaagtctt tcctgtaagt cccatattaa
    14941 ttgcaagagt tggttttaat cctcttctta tagtcttaag agatgccata agaccacgta
    15001 aatcaatagc tttactagat atctctccat tttgagactt caattgtaaa tctaaaaata
    15061 ttcctgcaaa gtgtattaat ttttcttcat cagcatctga aaattcattt tttaaaatca
    15121 tcattaactt atcttcttct ataggaggaa tatctattac cataaatcta gataccagtg
    15181 cttcattaag ttcttttgtt cctgcatatt cataattcat agtcccaata aatcttgttg
    15241 ctggatgtag attgactctc tcatacccag gtacatcaat tattcttcta tagtctaaag
    15301 ctgaatgtac cacaactata gcatcatttt ttgccatatt aatttcatca aacaccccaa
    15361 aacctccatt aatagcacac tcataaactg agcctcttct aagtttaact tcattgtcta
    15421 taaatgtatc tgtacctata agtgttgaac tgtctgtatt tatatgaaat gatgtattcc
    15481 actgaggtct attaaatatt tcccctagat tatcagcaag taagtttttt cctgttgcct
    15541 ttggtcccga taataatata ttttcttctt ctaaaatagc tgctatacac atagacaaga
    15601 tatctttacc ataaaaataa gttttagact ttgttactct gtattctaaa tctttttcta
    15661 aaccataata atttctaaaa tacagtacat cttcaataag tttttcatct accccttgca
    15721 cccttagatt atctattata tccatatttt tacccctttt atataaatag tatttgtttc
    15781 atttcttatc accattttag cataaaagga tataagctaa aattgatttt ttaatctccc
    15841 cattttaagt aaaaccatgc accattttat cactacaaaa ttaagagtac tacttactga
    15901 tatttcttga ttttgttcgt ctatacgtat ataatatatg ctgataaatt atgaggaaag
    15961 gatgattgaa atatggatgc ccagaagtag caaaaaaatg gggtatttct gaaagaagag
    16021 tacaaaaact atgtgaagaa agtcgcatac caggagtttc aaaactcggg tatatgtggc
    16081 tgataccaaa gaacgcagaa aagcctgttg atggtagaac aaaaagaaga aaggaagtac
    16141 aatatgaata gcgttttgat tatagatgat gacaaagaac tttgtgctct tatgaaaaaa
    16201 tgtgttgagc aagaaaattt atctgctatt actgcacatt gtggtgcaga aggtctacat
    16261 ttggtcgata aaaatagaca cacactctct cttgtaattt tggacataat gatgccagat
    16321 atagacggtt ttcaagttct gaaaaaaatt agagaaacaa gtaatattcc agtaattatg
    16381 cttactgcta aaaatgacga ggatgataaa gtttccggac tacgtttagg tgctgacgat
    16441 tacctaacaa aaccgttcag cataaacgaa cttatggctc gtgtcaattc tcttattcgt
    16501 cggtatacta tgttaaaccc tgcttttata acagacacag attgcataat aataaatggc
    16561 atgattattg acaagacaaa ccgaattgtt tctgtcaata atattcctgt tcaacttaca
    16621 agcaaagaat ttgatttact tgcttttctg gcttctaata agggacgggt gtttaccaaa
    16681 aaacagattt atacacaagt atgggaagaa gaatatgctt ttgacgatag taacatcatg
    16741 tctttcatca gtaaattgag aaaaaagatt gaaccagacc ccaaccatcc attttatatt
    16801 ttgacagttc gtggtgtcgg ttatcgtttt aataaggagg cttaacatga atacaagtgt
    16861 ctatctgctt ttgatattgg ttcttagcct gattattata gcgtaccttg tttctgtcct
    16921 gctacgtgtc cgaatacaac ttacacttat aaaagatgct ttagaggata taaaaaatgg
    16981 aaaccttaat cgacgtattt taacagatga aaacgacatt acaaaacaaa tttgctatga
    17041 tattaacgaa atagcaatca atagccaatt tcaacttatt cagcaaaaac aatcagaaca
    17101 agcctacaaa cagctaatga caagtctctc ccatgatgta aaaactccac ttgcttcttt
    17161 ggtcggttat ttggaagcag tagaaagtaa aattgttgta ggagaggaaa aagacgagta
    17221 tatccatgtt gcgtctaata aggctcatta cttaaagaat tttgtagaaa atctatttga
    17281 atgggtaaaa ttggattcta aggaacaagt ttttcatttt gatatttttg acttgaatga
    17341 attatcccgt aatattatat ctgattggat acctgtttta gaaagcagtc attttgaata
    17401 tgaatttgat ataccagaaa tagagtactt cttacgtata gacgcaaatg catatacccg
    17461 tatcattaat aacctattac aaaatattat tacccatagt agtggcaaca aaatgacatt
    17521 gagaattttt gaaaacaaag agcaagctca aattataata acagataatg ggaaaggtat
    17581 atcttcagac aatctcccac atatttttga aaggttgtac caatgcgacc attcacgagc
    17641 ttcaaaggga aatggtttag gcttggcgat tgcaaaggaa cttattaacg ctcataaggg
    17701 aaatattacc gccaacagca ctcccggtat gggaacagaa tttacaatat tgcttcctaa
    17761 agccctgtaa aagtacgagg ctttcttact ctaaaacaag ataaaagcaa gattatggca
    17821 aggttttggc aaggttcatg ttttataatt ctaattgtag ttgattttga cgttgtatca
    17881 actatctttt gtagaaacaa atttattaaa agtgaggaaa ttatatatga atgattttgt
    17941 gattgaaaca aagcaactta caaaaattta tgggaatcaa acagtagttg acaaagtcaa
    18001 tctccatgca aaaaaaggcc gaatatatgg tctgttagga cgcaacggag ctgggaaaac
    18061 cacaattatg aaaatgattt taggattgac ttcaatcacg tatggcgagg tagatgtatt
    18121 tggagagaat attaagggac gtgaaaaaca agtatatccc cgtatcggtg caattatcga
    18181 aacgccagga ttttatccta atctgacagg aacggagaac ttggagattt ttgcaaaact
    18241 gcgtggtaca gccgccccta attctgtaaa aagagcattg gaaattgtag gcttacctta
    18301 taaaaataaa aagctgttta gtgaatattc acttggcatg aaacagcgac ttggtattgc
    18361 caacgctatc ctgcatgacc cagaactgtt agttttagat gagccaacca atggtcttga
    18421 ccctattggt attgcggaga tgagaaagtt tattagggac ttgagcgttg agcatggaaa
    18481 gacaattctt atttccagtc atattctttc agaaatctca ttgttggctg atgatattgg
    18541 aattatcgac cacggtgttc ttttggaaga aagtagtatg agtgaactag aaaagaaaaa
    18601 cagtaaatat atcctgctac aagtttcaga tattccgaag acatcactta ttttagagcg
    18661 tcaatttaac acgaaggatt attccgttca agatgactac acgttacacc tttataacac
    18721 taatctggat atgggtgaaa tcaataaagc acttgtaatg cagaatgtaa ctgttattag
    18781 ttctcaactt tgtaatgata ctttagagga ctactttaaa aaaatcacag gaggagaggg
    18841 cattgcttaa acttattcaa acagaatttc ttaaactgcg tcgtaggaaa cttatttggc
    18901 tgatgttgtt gtctgctctc attatgccgt ttttcgcact actatacttc aattattttg
    18961 gtaaaacagg tgttgagcca ataaagtttt ataaatggtc tgcatttggt tatacacttt
    19021 ggattatttt gcctgttgtt ttgggtatat tttgcaccat gcttatacac gaagaaaacc
    19081 agtatgatat gctcaaacag ctttggattg tacctattag taaaatggga tactttttta
    19141 gtaaattttt cgttgttttg atttactcta tttgttttat gctgattaca gccgttgctt
    19201 ctgtgttatt tagtgtactt tctggatatg tcgtatttag ctgggcaagc attttatatt
    19261 tgttgaaaaa atgtttagaa attggtgtaa tcactgcttt tgtaatgttg ccgattttgg
    19321 caatcgctac atctcaaaaa ggatacattc tcccaatttg tatcacattg gtatatgcct
    19381 tttccggttt tgttataatg acaataaata tgtatttaca cccattatcc agtatgggag
    19441 ttattatcat gagaaataaa gacattccag gtcttatatt ttcacaggaa attaatgtcc
    19501 cattagcatt tctttgtatt tgtatttgga gtattgtatc agtattgttt gcaaacactg
    19561 cactaggaag aagaaagtga ggtgtagata atgtggaaat tactttgggc tgaatgtcaa
    19621 aaaattcgtc gttcaaaaat tgtttggatt acagtttttg catccattat gatagcagtc
    19681 atagtttttg caggagggca ggatgtatat aatgacccag atttacatta tggcttaaag
    19741 agcgtttatg acggttcacg gtatatagac aatgcaggtt ggtatatgga cgcagtacaa
    19801 ccttggtcaa ctttttttgt tcttcctgct gtcattgccc tattaggaag ctatattatt
    19861 tgtcgtgaag aagaggagga cactattaaa tccctgcgtc ttattcccat aaatgaaaca
    19921 aaattaacca tagcaaaaat gattattact tttatattca gtatacttct ttatttgtta
    19981 ctctttacta taacattttt gactgaatcc gttttacatt tttccgattt atcgacaaaa
    20041 ctggttttaa gttgcatgaa agagtatttt cttgatggca taggtgtatt ctttgcaatt
    20101 tctcccatta ttgccttggt atcgcgcatg aaaaaaggat attggcttgc attagtattc
    20161 acagaagttt attctattgc tggactattt gcgggtatgt caaatgtctt acaaaccttt
    20221 tttcctattg cggcaatatt taatatttct ggatactata taactactgg agaaaaaatt
    20281 atggggagta tcataagttt attactttgt gcctgccttt ctgcatttat acttaaaggg
    20341 ctaaaacaca atgaaaaaaa ttgaaatagg tatatcaaaa tgaaaaagat aaaaaaactg
    20401 attattctaa ttgtattctt gtttcttttt ggcatgttac actgttatac tgaatggaaa
    20461 ttcaacaata acccacctaa aggggtaata gaaagaataa atgaatggac tgataagatt
    20521 ggactggatt agtaacttat gttaattata gtgaatgtaa tattagcaag ttattgtttc
    20581 gtattttaat actataactt taaatatttg gttttaatct tccaaacgaa tataaaaaaa
    20641 ctgtcacaga ggcaacatat ttttgaatat ttataaagtg gtagttttga ttttcaaagc
    20701 tgccgccttt tcttctatat aagcaaactt atcctcaaca aagtgtaaaa catggtacat
    20761 tttaagaaaa aagtaccaca gacacaagca ataaaagaaa cgccgagaac ctacaaaaac
    20821 gtagtgctta tgcagattta cgacgccttc tattttctca accgtcttaa tgaccttgta
    20881 caaaagaaat ttttctaata ttttttataa tcttatcttg tgatgttttt tatgtataaa
    20941 tgacttaata acaaatccaa taaaaattat ggatattggt ataattagaa cgaggaattt
    21001 ataatcatta taatgtgaca ttattcttac ccaactctca gaaaaaatat atcctatact
    21061 gattaatgaa gtattccata tggttattcc cacgatagat gatattacaa aagttataat
    21121 attttgacct gctatacctg ctataaaaga tatgtatgtt ctacataaag ggattaatct
    21181 acatacacaa acagaaatag aagagtattt gttaaaataa tcttgagatg caaatattcc
    21241 tttctttgcc tttggacata tctctattat tttatttatg atagatttac ctccccaatt
    21301 tcccactgta taacaaaata tcgaaccaag tatacctgca attatactta aaattaaaat
    21361 tgtcagaaat ccaaaatgat ttcttgcagc aattgctcca cataaaggta aaacaacttc
    21421 gctaggtaat ggaaaacaag catactcaat cataattaat acaaatacag agattaatcc
    21481 atattgtaaa ataaaactat tgattacaga cacttactta tctatctcct ttcttattta
    21541 aaggttttaa gggtacatgt tctaaggatt ctcaccctat atttaaagaa tcaatctctt
    21601 taaactatta tatgtattta cacaaagcat ttataacata ctttaaaaat agtaccttaa
    21661 aataagaaac ttaataaata aaaaaa
//
LOCUS       DAMJYX010000062        21651 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJYX010000062 DAMJYX010000000
VERSION     DAMJYX010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.062"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(255..989)
                     /locus_tag="PC479_002384"
     CDS             complement(255..989)
                     /locus_tag="PC479_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2804924.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(2000..2638)
                     /locus_tag="PC479_002385"
     CDS             complement(2000..2638)
                     /locus_tag="PC479_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2804925.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(2641..3498)
                     /locus_tag="PC479_002386"
     CDS             complement(2641..3498)
                     /locus_tag="PC479_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804926.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(3500..3874)
                     /locus_tag="PC479_002387"
     CDS             complement(3500..3874)
                     /locus_tag="PC479_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2804927.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(5036..6226)
                     /locus_tag="PC479_002388"
     CDS             complement(5036..6226)
                     /locus_tag="PC479_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2804928.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(6242..8197)
                     /locus_tag="PC479_002389"
     CDS             complement(6242..8197)
                     /locus_tag="PC479_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2804929.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(8214..10280)
                     /locus_tag="PC479_002390"
     CDS             complement(8214..10280)
                     /locus_tag="PC479_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2804930.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGEIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(10646..11296)
                     /locus_tag="PC479_002391"
     CDS             complement(10646..11296)
                     /locus_tag="PC479_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2804931.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(12280..12615)
                     /locus_tag="PC479_002392"
                     /pseudo
     CDS             complement(12280..12615)
                     /locus_tag="PC479_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(12713..13393)
                     /locus_tag="PC479_002393"
     CDS             complement(12713..13393)
                     /locus_tag="PC479_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804932.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(13390..14316)
                     /locus_tag="PC479_002394"
     CDS             complement(13390..14316)
                     /locus_tag="PC479_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804933.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(14389..15306)
                     /locus_tag="PC479_002395"
     CDS             complement(14389..15306)
                     /locus_tag="PC479_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2804934.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(15306..15995)
                     /locus_tag="PC479_002396"
     CDS             complement(15306..15995)
                     /locus_tag="PC479_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804935.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(16403..16708)
                     /locus_tag="PC479_002397"
     CDS             complement(16403..16708)
                     /locus_tag="PC479_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804936.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(17865..18083)
                     /locus_tag="PC479_002398"
     CDS             complement(17865..18083)
                     /locus_tag="PC479_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804937.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(18084..18491)
                     /locus_tag="PC479_002399"
     CDS             complement(18084..18491)
                     /locus_tag="PC479_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804938.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(18577..19065)
                     /locus_tag="PC479_002400"
     CDS             complement(18577..19065)
                     /locus_tag="PC479_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2804939.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(19270..20367)
                     /locus_tag="PC479_002401"
     CDS             complement(19270..20367)
                     /locus_tag="PC479_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2804940.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(20740..20910)
                     /locus_tag="PC479_002402"
     CDS             complement(20740..20910)
                     /locus_tag="PC479_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804941.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(21082..21525)
                     /locus_tag="PC479_002403"
     CDS             complement(21082..21525)
                     /locus_tag="PC479_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2804942.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         7370 a         3599 c         2282 g         8400 t
ORIGIN      
        1 tgtatactaa agtttattta aagtattttc tttcccatta tactatgctg ttaaatattt
       61 ttcaactgaa gtattgtaga accttaaaat tactataaaa aactatattc taaaatactt
      121 gtaatactac gtatcaccct taacacttaa gtataatttt aaagcaaaat tcttattact
      181 tatatttaat tttgattcta catctttaga tataatttta agtaatttaa gaaattattg
      241 taaatcatat agtactactc aataattagg tttttcaatg ttccaattat gtttctttga
      301 tataatccac catgactaaa aactatcgtt tctatatcat aattacttat atttttaata
      361 gcatctttta actcttgttt gttgctgtgc attacatcaa caggcttaag ttctccattt
      421 tctacctgta acaaatctcc agcaataagt actttagact cttttaaata aagacatatg
      481 tgacctctag tgtgacctcc tgtatgtata acttctattt cctcacctag attcaatttt
      541 tcatggttat ctaatgacac atctacttta gtatatgatg atttaaatcc aagtcccata
      601 acatttagca tactaagcat cttatcatca attttatcta ccatttgttc caacatataa
      661 agtttaaaag gagtttcttc tcctgttata tagctgattt cacttttgta tgcatatact
      721 ttgatattat tcttagattt ttctcttaga taatttatgt ttcctatatg gtctatatca
      781 tgatgagtaa ttattatatt ttttaactta tcaaagctcg tgttactttt attaagagca
      841 tcaattatta aatcaccttg acccggaaga cctgtgtcaa taagtgttgc atcatcttct
      901 gtgtagatta caggaatata cataacttta tcttcaccga atacatttga tgaaattttt
      961 aaaacatcta atccctttgc taaattcata atttttctcc ttataattac ttaattagag
     1021 gtcaatttaa ttaaagaaat tgagaaaatg tagattttcc tctagttata ttttattaat
     1081 taaaaattat taatacctag tatatcactt aagttgaagg attactcgac ttgatttttt
     1141 tgttatttta tggtatagta attaatatag aagtaagtaa ttgcaagctt tgcaattact
     1201 tttatttttt tctaataact attttatttt tgataattcg tataaaatta aaagtaaagt
     1261 aatatggaaa atatgaatat agaaaaactt taataagaaa ataaattatg aataaataaa
     1321 agaaacgcaa tactgtatat agatgattat ttagaaaaat aaaatctctt ttaataaaaa
     1381 taagcttcaa aataaacttt tagttcataa agcttaggag gaactaaatt gagtgcaagt
     1441 gtatctactg ttcaaaataa atagttcata aagcactctt ttttatatta aatcaattat
     1501 attttcgtca tttcagcaat gaaaaagaaa cttatctcta ttttgagaca gcttcttttt
     1561 attaacttcc tactttttat tagttttcta aaattgaaaa gctttattta taggattcct
     1621 tagatttata ttttgacgat ataatttttt aaaaacatct taaaagatgg tatacaatac
     1681 acacaatcat actcataatt gaaaaataat caatggaaaa taaaattact ggatattatc
     1741 tattaaaaaa tacaaattga ttcacaagaa acatataatc tataatatta tgatgttact
     1801 ctctcgcacc aagtaacttt attttcaagt aaccttttaa attcatacca tctaacttca
     1861 ctcaataaaa atggcaatat aaatatgtac tctatttttt acttttaaat atctgacaag
     1921 ctttatatac tattggaagt aaaatacaaa gtaaaacagc ccaatctggt aaatgtggca
     1981 tataaaagtc ccccttacct tatacaagct ccttattact aaaaatttta atagaaataa
     2041 acattgaagc taacagtata attaaactta aaaggaacaa tacaatattc actatcatgg
     2101 atggatactg atatataaat ggaatttcaa aattgctgtg agctatagtt aatggtaata
     2161 caaatgatga agccatgatt atcaccacga aaaagaattt tgttgtttca tatcctaatt
     2221 tatattgaat aggcatataa actccataaa taatagaact tattaataaa acaatcaaaa
     2281 tatatgttaa gtcaaaagtt cctatttgtg gaataagtaa attttcaatc cagtaaacta
     2341 atgtacaata gataaatacc attgcaaata aaacatactt agattgaact aagccacgtc
     2401 taggataagg agttgaacaa agtaatacag atgcttttga atattgactc tcctttaata
     2461 atagatactg gcacagcata aattctgaaa atataatgga tagtacaaat cccaatattt
     2521 gtgcaaatgg aggtaaacgc cacaatgtaa ataatgggat tcctattgca ataaacaata
     2581 tcaacaatac atattttttt acaatcagaa tatctttttt aactaaatga atcaacataa
     2641 ttactctctc ctttcaatat atgcaagcat aatatcttca attgtaggac gctctattaa
     2701 aatatctggc atattttttt gcacttcatt aatttttgag gtaatcccta taaaaccata
     2761 acctgtaatt tctaaattta aaaataattt tttgatttca tcatttagaa attgaatatc
     2821 cccttttact tttctatgtt cgtccaatag agtatctttt tcacgctcta atataatacg
     2881 tccattgtca atcataacaa tcatatctgc tattttgtct aaatcagatg ttatatgagt
     2941 ggaaaagaaa acaccacgac cacatttttt catatatcct gttagtatgt caagtatctg
     3001 actacggatt agtgggtcta aaccacttgt aggttcatcc ataattaaaa actcagcttt
     3061 atgcgataat gctaatgcta atgcaaattt cattttcatt ccttttgaaa gtgtatcaat
     3121 cttttgtttt gggttaagat taaaacgctt catattttgt tgataatctt cttcacacca
     3181 attatcatat gaaggtgcaa taatactttt catttctgcc attgacaact cctcatagaa
     3241 atatgaatca tcaaatacaa ctccaattcg attttttaat tccttctcat ttccatcgaa
     3301 atctttacca aagattttta tttttccact atcacgcttt acgacatcca atatcgaacg
     3361 gattgttgtt gtttttcctg ctccattaac acctataaat ccagttatac aatcttctgg
     3421 taatgcaaag cttatattag ataatgaaaa atcggtataa gtcttattta agttataaac
     3481 ttctaaaata ttttccatat taatcctcct gatatagaat atccatcatt tcttgtaact
     3541 ctatctgtgt aattcctagt gcttttgcat ccttaatcat atctgccatt ttttgttcaa
     3601 ctatacgtct ctttgagtct cttagtattt ctaaatttcc agttgaaata aatgttccta
     3661 ttccaacttg gcgtgtaaga aagccttcat tttcaagttc ttcatacact cgtctaatag
     3721 tcaaaatact aacttttaaa tcattagcaa aatttcttat ggatggcatt gcatcccctt
     3781 ctttcagttc ttcagtaaat attgcttctt taatttgctc tttaatttgt tggtataatg
     3841 gcttatctga tgtgttagaa atgagcactt tcatttttta tcaccccctt tttatcattt
     3901 tcagtgtgat gttgttatat acacactata cacttagagc acttttttgt caatataaaa
     3961 gatgtaaaat attactatga caaaataaat atgaatgaaa tattagagat tgttgatagc
     4021 tataaacact ttttaaaacc acataagata gaatgtatta aatatttatt aaaagtaaga
     4081 attgaatatc taaaaaaaag aggaatcctg tatgaatgat ttgtttgatg ataaattaag
     4141 ttataaacta tttataaaaa acataaagga ataaatccat ttctaattac aaacatacta
     4201 aaaaatttag atagtaataa atagatagta ataaatagat agtaataaat gggatgaatt
     4261 tataagaatt tggatagagg aaagagttat tcaagagtgt agactagagg taaactctat
     4321 attaaaaaaa tttaaatttg gatattatga tgcatggaat atttgcagaa gtaaccttgc
     4381 tataagtata gaagattttt ttgattacaa aagataatac tctaaaatgt gaggatttca
     4441 acattcaata ctgtacttta aataatatca ctgcaaaata ccatccacct tttaccataa
     4501 ttgaataccc taattatttt aaaaaagaaa acattagaaa cagaaaacct tggatatata
     4561 aaataaaaaa tactaacttc taaaacacgg atactctttg ataaataaaa caaaaaataa
     4621 ttttaataat taaaattggc tatctcaaac tagagataag tctctttctc acttttaaaa
     4681 cgttgaaaat atattcgatt tcgtacaaaa aagagtgcct tagaactaaa aagttcactt
     4741 tgtggcactc ccttttttct cctcttttta attttttata aaaattcttc aaaaatatta
     4801 ttaaataccc tatattatac ataaatcaaa ttaaagttgc attttagcaa ataaaaacaa
     4861 attataacta ttagcatagc aaataaaaaa tctgctctct tataaaatca aattataatt
     4921 ataaaaagaa aatattctta taacaaatta gctaatataa aattatttta taaattaata
     4981 ctacttttta gcttctccta taaattaata ccactttttt aactttgaaa cctatctaca
     5041 acattggtac cataagacca gctaccacaa tagaaatcat acaagccata ccaccaacta
     5101 ccagcttagg cataatctta gacatcaaat atgctctttc ttcttccgtt tctccaacag
     5161 cattacacac ttcatttgaa attaaatatg tagttgggaa tcccaacatc tgacaaaacc
     5221 ctactccaaa agcaagaggt ttagaaccga taattttcca acctggtaac accttcatca
     5281 taaggaatat accaatgata gaagctgcaa acatacccac aattggtaca aaaagactaa
     5341 ttaaatctgt caaagaaact tttgctaaag aaggaataac agctgcaaac acaaccatgt
     5401 tgataaatcc cttgattttt cctttttcca aaatgtcttt tggcacaata ccaatacgtg
     5461 ttacaataac accaacaaca agacatacaa tagaataatt tacatgaata atctctgaaa
     5521 gttcataccc tagataagag aataatgcta taaaaaatat acaaacattt gtagaataat
     5581 atttatcgta tttttcacag aaagcttgtt ttacttttgt gcctgtattc tctgcttctt
     5641 tcttatcagt atcaacattt gctaaatcaa tgccttttga ttttgcttca tgatattcta
     5701 ctaaaagacg gtttgcctct tgtaacgctg ctcttgaagc tattggtgta ccaataaact
     5761 tctgaattgc aaatacaact gttgctgtag ctgctgcaag tgtcagtcct ttttctagtg
     5821 ctgcttgtgt cataatagta gtagcaggta aagctccatt tataactggt atgcttgtca
     5881 atactgttga ttttccaaac aaaggaatac ataatgagac acagactata actgctacaa
     5941 ttccaagcca tgctgtcaat actgttctcc attcgtctat aaattgttta acattcaaca
     6001 ttgttcccat actaaacata atcattggaa cactccaact agctgctgcg gtcatacctg
     6061 ctttttcaat aatatcagct ggtattgctt ttgtgacaaa tagaatcaaa aataaaagta
     6121 gtgtaacaaa tacagctgat acttttgctt ttgttttgct tgcaattaaa tccccaatag
     6181 caaataaaac taatacgaca ctaaaccata ataacatatc actcatagaa taaccccctt
     6241 attataaata tctttcaaac caatctacaa tagcatttgc tcttgcaatt ctattagatg
     6301 gtcttccatt tctagacaat ccatgtgtat ctccgtggaa caaatacatt tctgtatcta
     6361 caccaagctg ttttaatgct gtaaacattt ggaaagtatc ccccataaaa catctataat
     6421 ccctatcact atgtaagaac aatgttggtg tcttaacatt tttcacatat ttaataggtg
     6481 aatgattcca aagaacctcc tcatgattcc aaggcgtctt atctccacaa gtctgcttca
     6541 taattctatc cccaaagtca ataattaaag aagctgtaat caagttacta atacctcttt
     6601 gagaaactgc tgctttgaaa cggtctgtat gaccaattat ccagtttgtc atataaccac
     6661 cgtaacttcc ccctgcaacc cctaagcggt ctgcatcaat cccaggatat ttttcaatac
     6721 aagtatctac aaattccata agatgctcat agtcaacata cccccatctt tctaccattt
     6781 ctaagaattt ttcaccataa ccgtcaccac catgagggtt acaataaatc acatacattc
     6841 cctttgatgt tacacactgc ttcaagtgtg taaacatata gccatatgcc catttaggcc
     6901 caccatgaat gaacaataca gcaggatatt tttcgccttc tttagcatct ataggtggta
     6961 taacaaaacc atcaacttct tctttatttt ttgctacaaa cgtcaatttt tcaggtaatg
     7021 aaaccttatg agtatctaaa tatggtttag aaaaagcagt catttttttc attgtacctg
     7081 tagtcatatc aatgctatat atttctgcta actcataatc tttcaacatt actgtgtaca
     7141 tggtgttgtc gtaaatatca aaagatgtaa aagcatctac ttttgtgatg atacgtatat
     7201 tacctgtctt tgtatcaaac tcattaaatt ctgtcatagt tctgcaaatt cttgcaccaa
     7261 taaatttatc tcctataact ttattactac gtccaccacc atatactgca tctacatcca
     7321 gtaatgtagc atacatacca tctggactag caatcatatt tatttcacta gtactcaaat
     7381 caagtataaa aatatcatga ttctgtgtaa tagaacggtc taatgttgaa gcttgcaaca
     7441 ttactttatc tttgccaata tattttgcca aacttatttg gtactttcct tgtggtacaa
     7501 gttctttggt ttcaccactg tctatatgat actcccaaag tccagaaaaa ggagttgcac
     7561 aatcttcata aatctgacca gtatataaaa ttctgttttt atccgaagaa acatctatac
     7621 ctgctacttc acaaaatttt ggtgtaatgg cttttgttat attttcacct tcttgatata
     7681 attctaatgt tacacgtctt ctttgagaaa aattttctcc atctcttata aaaggtttat
     7741 ctgtaaatgt ccaataatct acaccctcta ctgccctatc tggttctgat tttttagaat
     7801 ccgttggatt ttttgttgtc agtaaccaca atttatttcc aagtgataca ggtacgctac
     7861 aagcttttgg aatagaaaat gctttttttg cttctcctcc ttctatagaa atagtgtaaa
     7921 aatctgttgt acctgctttt ggcttatttc ttttagatgt aaaaagtagt tctgtattag
     7981 aaatccaact aaatgatgtt acatcttttg tttgagtcat atgtatttgt ttttctgttt
     8041 tgatgtccat cacatataag tcatgttcat agcagtcttt ttcctcgttg atatttgata
     8101 taatataagc aatatgcttt ccatcaggtg aacaagaaat atcatgaggg aatatatatt
     8161 caaaaatatc tgaactttca atctttattt tttccatttt tatattctcc tttctactgt
     8221 cctatgttat ctttttttat atataccgaa aaccaatcta gcatttcaac taatctttgt
     8281 agcttttgtt ttggtctacc agaacgagac agttcatggt tttctccttc aaatagacaa
     8341 agttttgtag gacacccatg aattttcaat gctgaaaaca tctggaatgc ttcttgcata
     8401 tagcatctat agtctttttc accatgtaaa aataaagtcg gtgtaacaca tttatcaaca
     8461 taagcaagag gtgactgttt ccattgaagc tcgtggtttt ccatatgaga agcacctgtt
     8521 tgtcctttgc agaaataata accaatgtct gttgtaccct ccatgccact ccagtttgca
     8581 atagaacgct gagcacaagc aacagcaaaa cggtttgtat gaccaattat ccaattcgtc
     8641 ataaatccac cataacttcc accttcaaca gccaattttg ttttatcaat gtctgtatat
     8701 ttttcaagta cagtatctgt aaattccata aaatctatat aatcaatttc tccaaactgt
     8761 ttcctaatat ctgcaaaagc attgccacga ccttcagacc cccttgggtt acagaagaat
     8821 acaaagtaac ctgcacttgt aaatacctgt atctcatgca taaaatatgg tccatatgtc
     8881 aatcttggtc ctccatgaat cgccaataca gctggatatt ttttccccac ctcataatct
     8941 actggtttat atacgtatcc ttgtatttcc catttagaac ttccttttga ctctatgtat
     9001 tctggcacag aaattttata ttcctctaaa atatggtcat ttaaatgtgt tttcttacac
     9061 aatgttccat tctctactgt ataaacttct ggtaatccat ttcctaaaca acccacacat
     9121 acaatattgc catcttttac atctataaaa tcaactgaac ctgtctgtgt tactttttcc
     9181 tgttttcctg ttggaatatg tattctttca ataatgcagt ccatatctac tgtagaagtg
     9241 taataaatat attctccatc tacctttgtt gtctgaccag cacccatttt aacatcagat
     9301 gttactgtat ttccaataca atgatgttga tgccctgaaa gaagtttcat ttctttcgta
     9361 tcaatatcaa tagtataaaa atctccattt tctccatttc cataagataa agcatcagtt
     9421 aggtttagta tcacttgatt ttgatataat tcaaaacctt ttataatata cttatctttt
     9481 tctaatatac agacatttgt gtttgtgttt gtatcaaata catagacccc atttttcaaa
     9541 ccttttacat ctttaaattc ttgccctata tataagattt ttccatcact tattttcata
     9601 cctacaacat taaacatagg tgctgtaatt gccttcaact catttatatt actatcgtat
     9661 acatataggc gttttctttt tctgctaata tatccctccc cattgacagc ataaggaatt
     9721 tctttgaaat gttcataatg tcttttgtta taagcattta tttttttctg ttgttcttct
     9781 tctggcaatc cctctacaat aggtcggtca ttgtcataag aatcaataac tgcatacaaa
     9841 ccttttccta taggattcaa tttaccacct ttgataggga gtctaaaaag ttcttctgct
     9901 tctccaccat ttaaagataa agcatagtaa ctcatgcatt gcttaccgtt tttgacagct
     9961 tcttttactt tttcatttcg caaagcaggg aacaagatag tattttcatc ttcccaggca
    10021 tatgctccaa cagagtttat agacgttatt tgttttaatt cgccagtttt attgtttaat
    10081 atataaactt ggcttgtgta accattctct ttgatatcag ctttttttac aataaatgct
    10141 gtatatcctc catctggtga tatttggcaa tcacttataa attgatattc caaaaatgtt
    10201 tcactcgtaa atagcttttt catagtattt tctatccttt ctttagtaat ttttttcaaa
    10261 ctccctacca cctctttcat tttttatttt aaatttaaaa taaaaaattt taaatttaaa
    10321 ataaaaaatt ttataaaggt tattttttta ggaagccttc tggcttcctt gtaatatttg
    10381 taatttaaag tatatttaat ccatgcttaa ttcatgctta attcatactt aatctatact
    10441 taatctatac ttagtccata cttaatctat atttaattca tacttagact tatttacact
    10501 atatttagat tatatttatt agattatatt tattagatta tatttattag attatattta
    10561 gattatgttt aaactataat tagattatac ttagactaca tttagcaact taatagataa
    10621 gtatatatag taacttcaaa acttattatt catcttcaca gaatctttta atagttcctt
    10681 gaatatgatt tcttagttta tctactgctt tttttgtatc tctgtcttca atgaatttta
    10741 atatttcaaa atggtcttgt actgttcctt cataattacg attttttaat ggtcgaatta
    10801 aatagttcac tactataaat cttccttgta taccaacata tacatcattt aaaatctcat
    10861 tatttgcccc ttctacaatg gctttatgta attccaaatc tgctagtgca agttctacag
    10921 atttttcttg acgatagtac tcctcgcatt tatcagagta tctttttatt acatctagct
    10981 tttgttgaaa aacctcttcc tcttcttgca tcattttttc taaagcatat gtttcaatca
    11041 gtgttctcgt ttcacaaact tgttttacca tatcatattc atactctggt acgtacactt
    11101 taccatttgt cataatagta agcactccat ctttatttag catttttact gcttgtacaa
    11161 ctggtgttct gcttacttca aatttttcac aaaggtcatt tagtaatatt ttttgaccta
    11221 cagagtattc accacgaata agactttcaa taatcttatg atataaaatt tcatctaaat
    11281 ttgactttac tttcactact ctcaccttat ttctattctt ttgccatatt atattgaatt
    11341 actggacttt aattactttt agtaatattt ttaaagtaga aattcttatt tgatagtact
    11401 tttgtactac aatttaaatt attgtactgt aaatcatgct cttatactat agattaattc
    11461 attgtattgc aaatcaagtt attgtactat agattaaatc gttatacttc aaattaaact
    11521 attatactat agattaaacc attgtacttc aaattaaact attatactat agattaaatc
    11581 gttgtacttc aaattaaact attatactat agattaattc attgtacttc aaattaaact
    11641 attataccat agattaattc attgtattgc aaatcaagtt attgtactat agattaaatc
    11701 gttgtacttc aaatcaagtt attgtactat agattaaatc gttgtacttt aaattaaact
    11761 attatactat agattaattc attgttctgc aaatcatgct agtgtactac aaattaaact
    11821 ataaatgttt actatctctt aataatttga ataatatgtt acaaaaagta tagcaccttt
    11881 ttattttttt cgcaataaat aaaaaattta aaatttttta ttttttattt tcttatatta
    11941 tcaatttgaa atatgaaaga ttaagactaa tagaattgat tcagttaatt gaaaaaaata
    12001 ggtacctttt atgaaaattc taatgataag catttttctg atgtaatggt aaattataaa
    12061 tgataaaatt aaaaatcact tttagtttta tgaactgtcc ccatattaag aaacaactaa
    12121 gatacataaa aatagttata aagagatagt taaactatat ccactatttt atatagttaa
    12181 aatagtgggt atagtccaag ctaagaataa ctaagaaatt agcgttaaat agcatagata
    12241 aaaatacatt tatatataaa ctttttttgc tgcaaacaat tatttccata acattttata
    12301 tacttctcta aaatcttcta aacttgataa atattcaatt aaagggacat aattacctaa
    12361 actacaagaa tttaaggagt ttctatacaa ttcctcacta tcccaagata caatttttat
    12421 aggaagatta ttttctaaca tctgttttag cataacaaat ctacctatgt gaccattacc
    12481 atcttgaaat ggatgtatca attcaaatct ataatgaaat tcagttataa ctttaataga
    12541 tacttcactc tgactatagt accattcaat taattcatca agctttggct caacttcaaa
    12601 tggttgtgca atattattaa aactctaata atattaataa tagctgatat aatagcaaat
    12661 cattattatc ccgagaagat acaattaata actaccttcc tatccacagt atctagtcaa
    12721 tttcagtaac tttatgattt agtatataat atataccaat cacagttata ccaactataa
    12781 taaaaacaga aatcaaactg tcttgagttg tatttgtacc agctaaaata aattgtctta
    12841 aattgataaa aataattgaa ctgacaaggg taacaggtat tgattttctt atcattccaa
    12901 caacgaatgt ccatatacct aatgagcaaa atacaataat acttttaatt gctcctggta
    12961 tccaataaga taaaattgaa atatgaaaat ctccaataac taaattaaaa tacttatcca
    13021 acacaacaac gaatgtaaaa caacagatat atccaactat cattgatgat atcataagag
    13081 tcgtaatcaa aagtaacttt gataaaataa gttttttacg gtcaagtgga taagagaata
    13141 aaatcaagat tgttttattt gtatattcat ttattatata tgccacattc aacacagcaa
    13201 ataatataat atagtaaaaa cagaaaaata aactgattgt cctaaatgta acatcataat
    13261 catatattga agtagattta tcattcaatc cagtaaagac aaaaaacata cataagaaaa
    13321 tccccagcat tgaaaataaa aaatatcttt ttagcccaac tttttttagc tctagtttca
    13381 ttaaatgtat catagtgttt ctttacctcc tgtacctgta gtttggaaga agtaatcttc
    13441 taaagaactt tgtttacgtc caatgctatt tataccaatt tgattttgaa taaatatttt
    13501 ggatatttct tttacatctt ttcgtaaatc atatatttca atacaacttt ccgaagtaat
    13561 tttaaagtct tttattccaa attctttttc taacaagtat ccagcaagct ccacattatc
    13621 tacatctact tcaacataat ctgtttgata tttgtgtata tctgacatag aaatttcttt
    13681 aataagttta ccatcttgaa taattccaat agtgtccgca atttgttcta cctctgaaag
    13741 aatatgacta gaaataaata tagttattcc ataatctgta tttaacttac ggaataattc
    13801 tcgcatttct cttatcccct ctgggtcgag tgcatttata ggttcatcaa gaatcaataa
    13861 ttctggttta gtaagaattg ctctagctat tgcaagacgt tgtttcatcc ctaaagaata
    13921 ttttgaaact ttcttgtttg attgctttga caatccgaca cattccaata cctctgatat
    13981 atgttctttg ttataataac ccatatattc acagtgtaaa tcaagattct gtttagcagt
    14041 caaattctca tataaatacg gattttcaat aatagtacca atctttttta atatttcaaa
    14101 atcagtttct gccactttct taccgaacat aactatttct ccagaatttg gcttaacaag
    14161 atttagcaac attttcatta ctgtactttt acctgccccg tttggtccaa caaatccata
    14221 aatttctccc tttttcacat gaatattaac agcattaaca acagctttat ctttatatat
    14281 tttctttaat tgattggtct ctatgatata gttcatcata ctccctcctt tctaaaaaca
    14341 gtatacaaaa taaaattatc ttttctctct tttaaatcta acttttttct aacttttcaa
    14401 ttttactgta aaaactgttt ttacattagg tctactattt acataaattg aacctcccat
    14461 agagtttaca agcccttgag aaatagccaa acctaaacca ttgccttttt ttctgtcagc
    14521 agtataagct cttgtgaaaa tatgctcttt ttcagtttca gaaatacctg aacctttatc
    14581 ctcaatatct ataaaaacat gtttttcctt ttcatatagt gaaatcccta aaaatctacc
    14641 atctgaacca tattttatag cattatcaat taaatttttg agtatacgtt gtaatgcttc
    14701 tgtgtcaaca tttgcatata taggtctatc ttcaatatta acttcaacat taaagttttc
    14761 tttatctagc aaatcataaa actctaaaat aatctcatga cataattgtg ttagattgga
    14821 tttttgaaga tttaatatca tatctccaga ttggattttt gccatattaa acaggttatt
    14881 tattgaatta acaagctcat ttgaatttgt ttgcattttt tccacagttg ctctcattga
    14941 agaagataat tcttcttttt gactttgaag atataacatt tcaatataac cctttaatac
    15001 tgtgatggga gttcgtaaat cgtgtgatat gtttgtaaat atttgcaaca tcgctttttc
    15061 acttctatta taattaattt gcctgctgta aaacttttca agaagtttat ttatatctac
    15121 tgctgtttct tttacaatcg aaatatcaga tggtactaaa atataattaa tttcatcatc
    15181 atttgttatg tcacgaagtc ttgaattgat atacttataa tcactttcca ttttctttga
    15241 tttatttcgt tcttttaagt agagagcaaa tagtacaatc gaaattacaa ctaaaaataa
    15301 ctccattatt cttcctccat tttgtagcca attccccaaa gagttttaat ataattattt
    15361 ttttctatac catcagacaa tttatttctt aacctgttaa tatgagtatt tagcacattt
    15421 tcatttccat aataaggttc tttccaaata gcgttgtata attgttcctt tgaaaaagca
    15481 cgtccttgat tagtcgctag taatttaaca atttcaaatt ctgtatgtgt taagtttaag
    15541 tcaattccat tctttgaaac tacatatttt gatatattta taatcaaatc tttatactga
    15601 ataaccatat cttcagagtt tcttgaactg ctgtacactg ttgcacgtct aatattagct
    15661 ttaattcttg caagcaattc tacgattgaa aaaggctttg taatataatc atctgcacca
    15721 agattaagcc ctagcatttt atctgtgtca ctatctttcg cagtagtaat aataacagga
    15781 acaacactca cacttcgtat atattttaat acttcaaaac cactagactt tggtaacatc
    15841 aagtccagta aaataatatc aaatgaataa tttttaaata tttcaatagc tgtatttcca
    15901 tcaatggcag ttttaacaga gtaattctca ctaatcagaa aatcgtatac catttctcga
    15961 atattcttat catcttcaac caataagatg tccattatta ttcaactcct tttcgtcagt
    16021 atacttcaaa tgatatattt attaaaaagg gttgtcaata aataactgct tatgatattg
    16081 tatctgcaat ttggtgatac tcttctaagt taaagttctt ttcaataagc tttattgaaa
    16141 aaataattaa aatatagtat actctcttat ctttatctga aatatacata attttctttt
    16201 attaacatct acaattaact attttatatt ataaaacacc ttttaactct tgctaaaaaa
    16261 tattttatac ataaaaaaga aaccacattc tagttttata cagaacatag tttctttaaa
    16321 aataatcaaa tttggatttt aaataattaa aaacaaactt ttatttgttt ttaattttcc
    16381 aattttatag tagataattt agttaattag ttcttaaatg tttatcttct ttagtaaatt
    16441 tttttcttat taagaaatat gcaactatta caacaagttc tattgctaaa aatacaccat
    16501 aagtagctat agaatctatg ctatctatgc catttctaaa gaaaattaaa gcaattcctg
    16561 ctgctataat acctacagca tttttcccta tagaattttg accaaaacca taaaaagaag
    16621 ggaaatttcc ttttatttca atgaatgtca agattaatcc aatagctata aatagtactc
    16681 ctacaacttc cataatcatg ctaggcattt tgacccctcc ttattaataa ttaactacta
    16741 ctataaaaat atttattatt tttataatta tattatatat gcgattacat gtttttggaa
    16801 gtatatttgg aagtaatttc atatttattt aacttaattt ttttgtgata aaatacttct
    16861 aatttttata tagatattat tataaatttt ctagtttatg gagtcattca ttctataatt
    16921 cacaaatatg tattttttaa tctattaaag tattcatttt ctggcttata agcatggact
    16981 tcttatctat aagcttggtt tttcatctaa taagttattt attctttaat ctatgagctt
    17041 gatttttcat ctataagtca tttatttttt aatctatgag tttggttttt catctataaa
    17101 taagtcattt tttaatctat aagtttagtt cttcatctat aaatcattta ttttttaatc
    17161 tataagtttg gtttttcatc tataaataag tcatttattt tttaatctat aagtttagtt
    17221 cttcatctat aaatcattta ttttttaatc tatgagcttg atttttatct ataattaatt
    17281 cattctctaa tctattagct tggtttttat ttataagtca tttattctct aatctattag
    17341 cttggttttt acctataatt aattcattct ctaatctatt agcttggttt ttatttataa
    17401 gtcatttatt ctctaatcta taagcttact tcttaatcta taaatcattc attttttaat
    17461 ctattagctt ggtttttacc tataaatcat ttattctcta atctataagc tttctttttc
    17521 atctataaat cattcattct ttaatccatt agcttggttt ttcatctatg agtcatttat
    17581 tctctaatct attagcttgg tttttattta taagtcattt attctctaat ctattagctt
    17641 acttcttaat ctataaatca ttcatttttt aatctattag cttggttttt acctataaat
    17701 catttattct ctaatctata agctttcttt ttcatctata aatcattcat tctttaatct
    17761 atgagcttgg tttttcatct ataagtcatt tattctctaa tctataagct tggtttttta
    17821 cctataatta attcattctc taatgcataa gtaatacatg aaatttaaat attattttct
    17881 tcaataaatt caaacaactc ttctacagtt gtatcaaata ccttagcaat atccattgcc
    17941 agttttaaag aaggattgta gcgaccattt tctaaatgta caatagtttc acgtcttact
    18001 cctactgact ttgcaagttc accttgtgtc ataaggttct tttctctata ggatttaatt
    18061 tttgtcttta gtaatactga cacctaattg tatatcccct ttctgtcaaa atatataaat
    18121 agaattaact tcaacaagga ttgaataaac aaaatgacta ataaagacat tcctatattt
    18181 aaatttgaca catttatgtc caaagttaca agaaatatag atactatagc tacagaaaaa
    18241 aacaatactt ttattgcaat atcattaact cttgatagta tacattttga tgcctcatca
    18301 attaaatcat catacttttt attatagacc cactgagctc ccatagctaa tacccaaata
    18361 attataggaa gatattcaat actacttcct ttaaaaaaat acttatttac taatgtagct
    18421 ccaatacaat aagaaattcc caatatttct gaaataagtg ttgatttaat accagtttgt
    18481 tgtttagaca tataaaatcc cccttaaaaa gttatgtatt tataacacct cttgttataa
    18541 atatataact tttttgaata aatttcaata aaaatattat tttgcaacaa attcttttat
    18601 taattttaca ttagatagag gcactttaga tgtcactcct tgcaatgtca aatcttctat
    18661 attaacttca tttaattttg aacacagttc taagtcaaat attctttccc agcttacctt
    18721 cttttcaatt tgttcatctt cataaagctc tacagtaata tccattaaag gtaaatgcca
    18781 taaatcaaat tctgataata acacattgtc ttcacttatt tctatttcaa gtaaaacacc
    18841 tttttttcct ttttctaaat aaccactaca tcttaaatca ggtctcttcg tccaaaccca
    18901 tatcatacta tcatcaaatt catatattct atttgtcatt tgactataca tccatttgta
    18961 tggaatatca aagtcttcta atataaaaga ataatcattt gatagatatt tattgttttt
    19021 attgttattc caaaattcta aagtctgtat agtataaaat ttcatatata aatcaacccc
    19081 tttgtttttc tataagaagg agatggttca aacttttatt ttgaattatc tcctttattt
    19141 atacacctct ttgtacctat tatttatata tctctttata tctaccatat ttaaataaca
    19201 aagttttata ttaatttaaa tttaaaagag gaagctttgt atttatctag tgccatctca
    19261 atcatagtat tagttttgat tatagacttt ttcaacctct gatttattca aatcatctat
    19321 aatatcgcat atttcactca aattgtatcc tttatttttt aattttatga tgatattaaa
    19381 tttctcttct cttttccctt ttgcaatacc tttttcaata ccttcttgaa aaatttcttc
    19441 tttaaaattt ctacttttat tcataatata cgcacctctc tttaaattta ataaatagtc
    19501 ctcattacta cctaattctt ttaatctatc aagtgctctt ttaataacat aatccgtttt
    19561 agatagtatt ctaatatcta tatgagaagg attatcaata agtttacccc atattagaac
    19621 tctgtttgct atacaactgg atttacgaat ttttggaagt tctataaaat ataatgcagt
    19681 tttactagaa taaatatctg gatgcccgtc cattgttaat ctcaggcaat ttacataatc
    19741 ttctatgtga ttatatttta catgatttag tatatgtatt ccaacagttt ttccaatttc
    19801 tttatattct ttaccttctt ctaactttct accatgtaca cgactaagat aatattcagc
    19861 tctttccaaa taatcctctt caagagctct ttgcatctca atatttatat ggttacgttc
    19921 ttcatcttca gccaaaatat caagtctagg tcttttctca ccaatgtaat taattgttgg
    19981 gtcggtttca accttaatta ttctttttat ttgagtgaac tgtttgatat tcctattagc
    20041 atatggagca tctttgagga tatcatttat aaaatttgca agtaaatcgt aatcattatt
    20101 taaagtaaac aatgatttaa atacctcatc atactcaggc ttagcatacc catcttcagt
    20161 tacaacttct ccaacatttt cactagtcaa tatttctttt ggaattaaat ccttgatttt
    20221 atctaatttt tcattagaaa aatcacctct actaagtatg tcatttaatt ttttggtcca
    20281 ttctctgtcc ttttcttcaa ttatagttcg tttatactct tttgatgttt taataatctt
    20341 agatttttct tctaattttt ctttcatatc aacacctctt ttttattatt acccatttct
    20401 tcataccaaa gtctgaactt ctaagtaaaa tttgcattat tgaaataaga accttataaa
    20461 cttatatatc attactaaag ttaagacttt aaagagaagc acttagatta caatagattt
    20521 tagatagctt tctttttaaa actattgttg ataaatgaat gaatataagg gtatgtatta
    20581 aaatagagat actcatcttt ttcattgttt aaataatgaa aatatatttt atttaataca
    20641 aaaaagagtg ccttataaac taaaagttca cttcgagaca ctcccttaca ttttgtttct
    20701 taaactttac aatattacat acttatttat taatttaatc tatgcatact taacccaata
    20761 ataaagtaca tatatccaac tcattaagcc atgaaaaata gcccatggaa tagatttcca
    20821 tgcagtataa gaaataatca ttgctaaaca agaaccaaaa gaaataccct ttttaacagt
    20881 tgattttata gtattattat ttgaatccat aattaatcct ccaaaattca aaataaacaa
    20941 gttattatat atcttatttg aatataatac aatataacag cttctaaata aattatactc
    21001 ttattataca taatttataa attataattc cgataattat atgaattaat taattatatt
    21061 aacctaaaaa tattatccta tctaaaagtt atatgataga tacaacatgt caacacattg
    21121 taattctcct tcaaagattg gttctggata attatctaca aaaaagtttt taattttatg
    21181 tgaataaaca aatccaaact tctcataaaa aggaactccg ctttcagatg taccaacaac
    21241 cattgatgaa tattttttac tataaacatc taaaagatga taaatcatct ttgaggcata
    21301 acccatattt tgaaaatgct catatgtagc aatgtttttt aattcacata tttctttgga
    21361 aatttcattt acaactgcta tacaagtaag ttctttatta tcatacaatg cgtacatagt
    21421 tcctttattg agatatttat caatcatttc ttcacatggg tcggctaaca ttaaaaggtc
    21481 aattcgactt tttttattta tttctatagt ttttatttct atcataagtt ctccaccctc
    21541 actttattca aaccttaatt taacaaactt cttacagtac ttatttaatt cattttgata
    21601 tatattttta ctttttaaaa tatagtgata attccctaga atattaatac t
//
LOCUS       DAMJYX010000063        20834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJYX010000063 DAMJYX010000000
VERSION     DAMJYX010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.063"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..842)
                     /locus_tag="PC479_002404"
     CDS             complement(<1..842)
                     /locus_tag="PC479_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2804943.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1103..3145)
                     /locus_tag="PC479_002405"
     CDS             complement(1103..3145)
                     /locus_tag="PC479_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2804944.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3297..4268)
                     /locus_tag="PC479_002406"
     CDS             complement(3297..4268)
                     /locus_tag="PC479_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2804945.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4269..5246)
                     /locus_tag="PC479_002407"
     CDS             complement(4269..5246)
                     /locus_tag="PC479_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2804946.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5465..7024
                     /locus_tag="PC479_002408"
     CDS             5465..7024
                     /locus_tag="PC479_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2804947.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7130..8089
                     /locus_tag="PC479_002409"
     CDS             7130..8089
                     /locus_tag="PC479_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804948.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8092..8904
                     /locus_tag="PC479_002410"
     CDS             8092..8904
                     /locus_tag="PC479_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2804949.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9120..10022)
                     /locus_tag="PC479_002411"
     CDS             complement(9120..10022)
                     /locus_tag="PC479_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2804950.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10377..11555
                     /locus_tag="PC479_002412"
     CDS             10377..11555
                     /locus_tag="PC479_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2804951.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11564..12220
                     /locus_tag="PC479_002413"
     CDS             11564..12220
                     /locus_tag="PC479_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2804952.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12220..12885
                     /locus_tag="PC479_002414"
     CDS             12220..12885
                     /locus_tag="PC479_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2804953.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            12990..13766
                     /locus_tag="PC479_002415"
     CDS             12990..13766
                     /locus_tag="PC479_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2804954.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13828..15162
                     /locus_tag="PC479_002416"
     CDS             13828..15162
                     /locus_tag="PC479_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2804955.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15658..16590
                     /locus_tag="PC479_002417"
     CDS             15658..16590
                     /locus_tag="PC479_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2804956.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(16982..20521)
                     /gene="nifJ"
                     /locus_tag="PC479_002418"
     CDS             complement(16982..20521)
                     /gene="nifJ"
                     /locus_tag="PC479_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2804957.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7204 a         3265 c         2924 g         7441 t
ORIGIN      
        1 tataaattca gtacagatat aactcttttg atatgtaatg ttaaataaag tagttcatct
       61 tgacttacat agtaatcata attcttttct acatgagttt ttattttata tgcacaatca
      121 taatcctttt catactttac tttaattata tctataattt cattatttgt atcattgatt
      181 ttttcttttt ttactaatcg ttttgcaaag aattttaaat gagtcagaag cctatcataa
      241 tttatatcat cttctttaaa ttcaactcta taataatatc ttataatatt tagtatgtct
      301 tttacaattt tagtaattaa cagtgattcc tttgaacttc ccttatagtt tgaatttact
      361 atatgcattg ctataaaacc agcttcatct actggaaatt tcacatcaaa ttctttattt
      421 atataatcta cagcccattt cccaatctca tattcttttt tatgtattct ttttatctca
      481 tgtagtaaat cattctttat agttatgttt tcatgatatc ttttaattgc aaatgcaata
      541 tggtcagtaa gtgctacata aatatattca taaagtttag tattcaaatt ctcctctgca
      601 taagcaatta tttcagtact aacctcaaaa aatttctcat caacctggct tgctagttta
      661 ctaattttat ctaaaaagct ttcatcttgt attacaaaag ttctttcaat tttactttta
      721 tctatttctt gacctttctt tttttgaaac ccaattccac atcctgttaa tattaattct
      781 ttttttatat tttcatctaa aactaataca acattattat tataaatttg ttgaataatc
      841 ataatcaata tctctcccaa aatacaattt cccttattca taaaaaaata cctaaattaa
      901 gatacaattc acctgtatac cttaacctag gtattgcctg cttaccagta acaatccagc
      961 ttgttaacgc ttatatattg tattcaattt atggttttat tctattataa ataaatatat
     1021 atgtcaagta aattattgaa aactatttcc tattttttag tattaatttt aacatcaaaa
     1081 atcaaatttt attccattat atttatgatt ctatcatcat catttcatca tagagaatat
     1141 tttccaataa ctctttagcc acttctcttg atatttgctt agtttcaact aattgttgaa
     1201 cactgtctct ttgactttgt atcgccatta ctctgtagta ttctactctt gcctcaaact
     1261 tatcatcatt ttgaatcttt ctagtaatcc aattcaaata acgattatag aatctgattg
     1321 ctattaaggc ctcatgtctg ttatcttcat cagtattttt atttataaat gatatagcag
     1381 ttttagtaga aagtgcatat gcatggtgaa actctttgat tatttcttta ttctcagata
     1441 ctatttttgt aattttttct tttacttctt tctttacttt tctatttata atccacatgc
     1501 ttatttttaa ttttacaatt ttaaatatag atttttgaac agattttctt gattttgtaa
     1561 tataaaatct atacaattta aaggctctat cagaaatttt accatcttct aatagtttca
     1621 aaacagctaa taatccctgt tggtctgctt ctgcaatcat catacttata agtttattat
     1681 ctggagtatt tagtattcct ttttctgagt ctaccaactg ttcatttaaa tcatgtataa
     1741 ctggttgtac ttcttgtgct ttttcattac taagttttaa aatggcttct ttcaaaatta
     1801 gtttatatgc ctctttatca gataataaag gttctgactc ttgtacgtta ttttttaata
     1861 atcttggcag taaaactgtt gccaatacta aacttataag aataacttct gatgatataa
     1921 ataaaacagt atctctcatt ggaaatacat ctttatcagc caaataaaaa ggtatagata
     1981 aggcagttgc tagtgtaaat gtaccatgaa caccacaagt agcaactatt agtgagtatt
     2041 tacatttaga aatactctct tttacaactt cttcatcctt aaaacctaaa aaacctacaa
     2101 aaaaattatt taaaggattc tttttaggtt ttataaaaga attatgccat aaatataccc
     2161 atataaatct tataacaaat aaaataagta caattaaagt agaaataaaa gtcaattcca
     2221 atgccatatt ttcattttta ctactaagtc ctgtatatat acttggtaac aaaaaaccaa
     2281 gcagtgtaaa gacaaatcca ttcaatacat actccaaaac ataccaagta ttgtctgata
     2341 ttatccttaa tttagtagtt gtattttgta aatgctcttt ttcaattcca tgagctatcc
     2401 ctgccattac aacagctagg attccagata ctcctatttc ttctgctaca aaatatacaa
     2461 aaagtggagt cattatttgt ataactatta ccattggaat ttcttccaaa ttccacttgt
     2521 gtaaactaag tcttattttt ataattatgt atcctaaaat acttcctact attattcctc
     2581 caaaagctgt aatcataaat tgaatccctg cctcttctat tgaaaaaact cctgtaactg
     2641 tggcaagtac tgcaacctta aatgctacta ctccagcagc atcatttaat aaagattcac
     2701 cttcaagtat actcatcata tttttaggaa aatttaatcc ttgtgtaatt gattttactg
     2761 ctactaaatc agttggagat attactgctg ccaaagcaaa tgaaacagat aatggcatcc
     2821 caggaataag aaaatgtata aagattccac ctgcaaacac ggttattaaa actaaaccaa
     2881 atgcaagaag caaaattggt tttctgagct ctaatagctc ttttctcgat acattttgtc
     2941 cttcactgaa tagtaatgga gctatgatac atattacaaa tatttctgga ttaaatgaaa
     3001 atgagaattt aaatggcaaa atagacaata taatccctag tacaatttga tatattgcaa
     3061 gaggaatatt actccacttt ttgctaaata cagttgaaat cgttgtacaa accaataatc
     3121 ccaacaatgt tactaaaatt gacataacta cctctctttt caataataat attatagatt
     3181 aaacttatat atcaactaac ttaatattaa cagcatgaaa tcccatctta atcaaaaaaa
     3241 ttttcagttt attctattat tcttattata tacatcaaat ttaatttttg aaatatctat
     3301 actagatgac atgcaacaaa atgatttctt gatatttctt ttaaaactgg aatctctttt
     3361 ttgcatattt ccttacaata agggcatctt gtatgaaact tacatccact tggtggattt
     3421 gttggagatg gtatgtctcc ttttaatata attctatttt tcttaattgt agggtcttgt
     3481 attggtactg cgctcataag cgcttttgta tatggatgtt gagaatgtaa gtaaagttct
     3541 cctttagttg caatttctat tatagaacct agatacataa cacaaactct atgtgcaata
     3601 tgatttacta cactcaaatc atgagatata aataaatatg aaagtttata tttatcctgt
     3661 aaatccataa gtaaatttat aatttgagat tgaatagaaa catctagtgc agaaacaggt
     3721 tcatcacaaa ctataaattc tggatttaat gctaatgctc ttgctatacc tattctctgt
     3781 ctttgacctc cagaaaactc acctggtttt ctatttaaat ggtacttaga aagaccacaa
     3841 ctttctacaa catttaatat attatctttt atttcattct ttggcactaa attatgttct
     3901 ataactgcct cggatattat ttgttccaca tttaatcttg gatttaatga actatatggg
     3961 tcttgaaata tcatttgcat tttgggtctt aactttctaa gttcttcctt ctctaactta
     4021 tatatatcta caccattaaa ttcaacttta ccttcagttt tttcaattaa tctaagaatg
     4081 gttcttcctg cagttgattt tccacatccc gattctccaa ctatagccaa agtttctcct
     4141 ttttttaaat caaaagaaat tccatctact gcctttacac atttaatttc cttatcaaat
     4201 aatccagatt taacttcaaa gtacttttta agaccttcta cttttaataa actctcttct
     4261 tttttcaatt atatctcctc ctttttatat aaccaacatg caatactatg accactattc
     4321 aattctatta aaggaggtat ctgagttcta catttatcac atactttttc acatctatca
     4381 ctaaaataac atgaatctgg catcccaatt ggatttggta cttgtcctgg aatcgaatac
     4441 aatcttttat ctttgttttt tccaagagca ggctttgatt ttagaagtcc aatagtataa
     4501 ggatgttttg gatttttaaa taattcaaca acctctgcca cttctataat tttgccagca
     4561 tacatcacta atactttgtc acacatttct gcaactactc ccaaatcatg tgtaatcatc
     4621 attatagatg tgtttaattt ttcttttata tttttcatta tatctaaaat ctgagcttga
     4681 atagtaacat ctaaagctgt tgttggttca tcagctataa gtaattttgg attacatgat
     4741 aaagccatag ctatcattat tcgttgtctc attccacctg ataattcatg tggatatgaa
     4801 ttatatattt cttcagctct tggtatttct accagtttta tcatatcgat tacctttttc
     4861 ttagcttctt cctttgataa atcttgatgt atcataatac cttcaatgat ttgacttcca
     4921 atagtaaacg ctgggtttaa agatgtcatt ggttcttgaa aaatcattga tatctcattc
     4981 cctcttattc ccataagttc atcttcattc atctttaaaa tatctttgcc tcgaaagatt
     5041 atttctccac cttctatttt tgatgttgga agtaacttca tcaaagacat ggctgttaca
     5101 gacttaccac atcctgattc tccaactatt ccaatagttt caccttcatt tatattaaaa
     5161 cttacttcat ttacactctt tacaatccct ttatctgtat gaaaataaac ttttaaatct
     5221 ttaacctcaa ttagtgcttt actcattatt ttctcctctc agcaaaatta cttaagataa
     5281 catacacctt ttgattgtat tttataaaaa tttaaattac aacattttct tacattttat
     5341 tatttttttc tattaattat aataatataa tagttaattt ctatatatat tgatttaata
     5401 atgattacta ttatacaata aaaaatatgt gttaaatatt attttatgga ggttttataa
     5461 gaatatgaaa tttaaaaaat tggcatcatt aatcttagtt tcatcattaa tgcttacatt
     5521 tacagcctgt gcttccaatg ataaagacaa gtctagtgga agcaaatctg gtggaactgt
     5581 tgtaatacct atggcaagtg acccagatat aatcaatgga gcttttgcaa acgttaaaga
     5641 agactctctt gcatcaaata tagtttatgc tcctctttat acttatgaaa aaggaaattt
     5701 agtaaattat ttagcagaaa aagtagattt taaagatagt aaggaattaa ctataaaatt
     5761 aaaatccaat ttaaaatggc atgatggaaa gccaataact gctgaagatg tcttatttac
     5821 atttaataca gttttagacg aaaaacaaaa ttcaccatca agacaatact tattggtggg
     5881 tgagaaacca gttaaagtag aaaaaattga tgatttaaca gttaagatta ctttaccaac
     5941 tgcaagtgaa tctttcttat atggtatatc taaaatttct ccaataccaa aacatgtgtt
     6001 tgaaggagaa tcaaatatag ctaagtcaga aaaaaataat aatccagttg gttctggtgc
     6061 attcaagttt aaggaatgga aaaaaggtga aagcatagta tttgaaaaga atgcagatta
     6121 ttttggtgga gaatctaagg cagattctat agcgctaaaa ataattccta atgaagcatc
     6181 tcaagaagca gctttaaata atggcgaaat ttcacttatg aagacatctg ctgaaggata
     6241 tgaaaaggca aaatcaaact caaatttaca aacttatact tatagtgaag aaagattaaa
     6301 ctacattgta tttaaccaaa atatatcaaa catggctaac aaagaagtaa gacaagcctt
     6361 atcatatgcc ttaaatagaa atgaaatgat agaatcagca tatggaaaag aaggttctgt
     6421 acctgctaaa tctatattag ttccagaagc agacttctat acagaagaag gtgttgaagg
     6481 atatgaccaa gatacaaata aggctaaaga cttattggat aaaagtggtg ttaaaattga
     6541 taaattaaaa ataggttata acacaggtag atttgggcat aaaaactatg ccttagtagc
     6601 tcaacaagag ttgaaaaaaa ttggtataga agctgaaata gttccttatg aaagtaaggc
     6661 attcttcaat atactattta gtaatagcac tgaatgtgat atgtatgtaa atggttatgc
     6721 ttggggatta gaaccaaacc catatagagg tatgtttgaa actggtcaat attgcaacca
     6781 aactaaatat tctaatgctg aaatagatgc tttatgggaa aaaggattta ctgagttaaa
     6841 caaggaaaaa cgtgaagaaa tatataagca aatacaacag gatatatcta aggatgcgcc
     6901 tatttacact atagattatg aacaaaattt aatggcagct caaaaaaatc taaaaggaat
     6961 aaaagatgct aaaccatctc ctgcaatatt atttgaagat tggtctaaac tgtatgtaga
     7021 ataattgatg taaaatttta tattattaat tagaaaagga gatgtctaaa aataatattg
     7081 ttttattatt ttaaaacgag acatctcttt taattaggag aatttactta tgttaaaatt
     7141 aatattgaat agagtaaaag ttttaatacc tatgttaatc ttaatatcta tattatcatt
     7201 tggactttta gaattagctc ctggtgaccc agcagatgca tatataaatc ctttaatgtc
     7261 ttcacaggat attgaaaata taagggttaa tatggggctt gataaaccag catatataag
     7321 atatttaaac tggttgaaaa acactttaag tggaaactta ggtatttctt atataaacca
     7381 tatgcccgtt acagaacaaa ttatggaaaa aatgggaaat acatttattc ttatgggtac
     7441 ttctttgata ttttcaatat tggtagccat acctcttgga atatttttag ctgtaaataa
     7501 aaattcaata actagtaaag tatcatctgt ttttaattat ataggtgttt ctataccttc
     7561 tttttggata ggtatgatac tcatttcaat tttttcagtt aaacttaaca ttttcccatc
     7621 aggaggtatg catactattg gaaatgatag tattggagat ttagcaaaac atttagtttt
     7681 gcctgtaata actttaggtc tatataatac tgctattttt acaaattatg tagaagctag
     7741 tgtaaatgaa caattaaaaa aacaatatgt tattacagca agagcaaaag gattatcaga
     7801 aaaagtcata ctatttaaac atgtcttgaa aaattcactg acatcattgg ttacaatcct
     7861 aggcatgagt atacaaaaat tagtcactgg tgcatttgtt acagaagtag ttttctcttg
     7921 gccaggtatg ggaagattaa tgattgattc aattttttca agagattata ctgtaattat
     7981 ggcaataact atgttatcag cactattttt aattttaggt aatttagttg ctgatatact
     8041 atacttatta attgacccta aaataaaatc tagcaaagga ggattctagt tgtgtttaca
     8101 agtatcttaa aaaaattaaa aaaagataaa tcctttgctt ttgcatgtat tactcttagc
     8161 ataataatca tattaattat agcttctttt atattaaata tagactctaa tgcaattgat
     8221 actacttcta aacttcaaga acctagctta aagcatatct ttggtacaga cgaacttggt
     8281 agagattact tctctagagc tctttatggt ggaaggattt cacttagtgt tggtatttta
     8341 tcaatgctta tttctgtagt atttggtact gctgtaggtg ttattagtgg atatattgga
     8401 ggtaagctag accaattctt gatgagatta gtggatatgc ttatgagtat accaagtttc
     8461 ttagttttga ttgtattaaa tacttatcta acacctaaag tttccactat tatactaatt
     8521 attggattct taagttggat ggaagtttca agaattgtac gtggcgaaac attaaaaata
     8581 aaggaaaatg aatactgtct agctgctaaa gctactggta tcaaaactag aaatatacta
     8641 ttaagacata tagttttaaa cttaaaagag actattgtag ttgctggttc gttaaatatt
     8701 gctaatgcta tcttaactga atctgctctt agttttttag gtcttggaat ccaattgcct
     8761 cttgcatcat ggggtaatat gcttcaagat gctcaaagac atatttttga caagttatat
     8821 ttagctgtat ttccaggatt acttatattt ttaacagtca tgagtttgac tataataagc
     8881 aaaaaatttt ctagtaatac ttaacaaatt ttatatagaa aataaaaaca gtgattatct
     8941 aaagatatac actgttttta tttttcgttt aaaaaaatac atattttgta tattataact
     9001 ttaaatacta ctctaccatg ttatgcaaag cgtattattg ctctcaaaca tatagatgaa
     9061 aaactaatag tttattataa taaaaattga tttatttatt tgttatttta ataacatttt
     9121 taagagttct ttttactaaa gaaatctata ataaatttaa atacatgttg gtctacatgg
     9181 ctataacgtt cttttttagg tctacataat ataactttcc atgtaatagg atgattaaaa
     9241 ggcttttcta taattttgct attttcaaaa aaatcatgaa ctggtgatgg taaaattgtt
     9301 ataaaatcag aatttgtggt tgatagcaac aaaaaatccc atgaaccaga cattatatat
     9361 ctttttaact taacattttc atcagtaaat ttttgcatta gcttatgatg tatcataaaa
     9421 gtaggattaa atattgcaag taactgctca ttcaaatcgt tccaatctat cttatccttc
     9481 ttagcaagtg gatgatttat atttaaaaat gctgtaagtt catcttcttg aagcacatgt
     9541 tcagttacta cacttttatc aattttatgt ggatgtaaca atactgcata atctaattcc
     9601 tgtaagataa gcattctatt taagtcatat gcacccttct ctactatatc aaattctata
     9661 tctggattat ttgcaactaa ctgtgcaact acatctgaaa atgctattcc aagtataagt
     9721 ggaggtattc ctattctaac ttttccttta aattgaactg aatcttctct taaatcttcc
     9781 atcatatttt tgtattcatt tatgatatgt tctgcattta cataaaatct ttctcctgct
     9841 ggtgttaacc catttaacct gcctttatac ctttcaaata aatatacatc ttcactttct
     9901 tcaaaatttt ttataatttg acttaatgct ggttgagata tgtgaagtgt cttagatgct
     9961 attgataaat taaaaccaga tttcactatt tcaacaaaat atttcaactg ttttatatcc
    10021 attttaaacc cccttcccca aaagtataat ttagattata ttttttcttc taatgatatt
    10081 ttataggaaa atcaacataa tttcattaat attttaacaa aatatttttc taaatctact
    10141 ataaattttt atcctataaa tttttcttat acactatata aaaaattaga cttagacaaa
    10201 acgctttttt cgccgtaatc tataattaga aacaggaaaa cctaaagcat taggaataaa
    10261 agtaattgaa atacttaaaa acaagcttgt attaaaaaag tactttatta tagtgttccc
    10321 taatttcttt attcagaaaa tttttaatta tcattcaaga aaggtaacta ttagatatga
    10381 aagatgtagt aatcgtatca gcagtcagaa cacctatagg aagttttgga ggtgtattta
    10441 aaaatacttc agctgttcaa cttggaacta tcgctgttaa agaagcgata tcacgtgtaa
    10501 gacttaattt atctgaaata gatgaagtaa taattggaaa tgttcttcaa acagggcttg
    10561 gacagaatgt tgctagacaa attgctataa atgcaggtat accaaattct gttccaagct
    10621 acacagtaaa taaattatgt ggttcagggc ttaaaagtgt ccagctagct gcccaatcaa
    10681 tcacatctgg tgaaaatgat gtggtaattg caggtggtac tgaaaatatg agccaagctc
    10741 cttatatcgt tccaactgct cgctttggta gtaaaatggg tgacataacc atggtagata
    10801 gtatgctaac agatggcctt attgatgctt tcaatcagta tcatatggga ataactgcag
    10861 aaaatattgc aactaaattt gaatttacta gagaaatgca agataaattg gcattagaaa
    10921 gtcaaaataa ggctgagaat gctataaaaa acaaccgctt taaagaagaa atcgttccag
    10981 tagatgtttt aatacgtcga ggaaaaatag aaactattga taaagatgaa tatcctaaat
    11041 taggtatgac atttgaaggt ttatctaaac ttaaacctgc attcaaaaaa gatggtactg
    11101 taacagctgg aaatgcctca ggtattaatg atggtgcggc tatgttaata ctaatgagcc
    11161 aacaaaaagc agacgaacta ggtattagac cacttgctaa aataaaatcc tatgcttcag
    11221 ctggtgttga acctgaagtc atgggtactg gtccaatccc tgcaactaga aaagcactga
    11281 aaaaagctgg acttagcatc aatgatatag acttaataga agcaaatgaa gcgtttgctg
    11341 cccaagcatt agctgttaaa aatgaacttc aaatagattc atcaaagtta aatgttaatg
    11401 gtggtgcaat agcattagga catccaatag gagcaagtgg agctagaata cttgttacct
    11461 taatttatga aatgcaaaaa cgtaaagttg aaactggact tgcaacactt tgtattggtg
    11521 gtggtcaagg aatatcaatg gtggtttcac gataggagga aatatgaata aaatagttag
    11581 cattgatgaa gctctatctc atgtaaaaga tggagataca ataatggttg gtggattcat
    11641 ggccaatggt tcaccagaaa atttaattga ttatttatgt agtaaaaata taaaagattt
    11701 gacactaatt tgcaatgata ctggctttat tgacaaagga gttggcaaaa tggttgtaaa
    11761 taagcaattt aaaaagataa ttgcttctca tgtaggatta aataaagaaa ctggaagaca
    11821 aatgaataac aatgaaacag atgttgagtt agttcctcag ggaactcttg ctgaacaaat
    11881 acgtgcagct ggttatggtc taggaggtat actcacaccg actggtattg gtactcttgt
    11941 tgaggaggga aagcaaaaaa ttgttgtcga tagcaaagaa taccttcttg aaaaaccaat
    12001 ttatgcagat gttgctcttc tatttgcatc aaaggtcgat aaagcaggta atctagttta
    12061 taaaggctct atgaacaatt ttaataattt aatggcatct gctgccaaaa ttacaatagt
    12121 tgaagcagat gaaatcgttg aaattggtga tattgaccca aatgaagtta atacaccagg
    12181 tatttttgtg aattacatcg ttgaaggagg taatttataa tggataaatt agaaatgcaa
    12241 gaatatattg caaatagagt atctaaagaa ttaaaagacg gagccgttgt aaaccttgga
    12301 ataggtctac caaccaaagt agcaaattat attccggata atgtaaacgt tatcttacaa
    12361 tctgaaaatg gctttttagg tttaggttca gccgaagatg gaaattcaag tgatgaaact
    12421 attgtaaatg caggtggtca accagttact atacttcctg gaggatgttt ctttgatagt
    12481 gctacttctt ttggaataat cagaggaggt catgttgata taactgttct tggcgcactt
    12541 caggttgaca aatatggcaa tatagcaaac tatatgattc caggtaagat ggttccaggt
    12601 atgggtggag ctatggacct agtaactgga gctaaaaaag ttattgtagc aatggaacat
    12661 acttcaaaag gttctgcaaa aatattaaat aattgtactc ttccacttac tgcaacaaat
    12721 gctgtagatt tgatagttac tgaaatggga gttatggaag taacatcaga tggaatttta
    12781 cttaaagaaa taaatccagc tttcacatta gatgatgtta tttctgctac tgaagctcct
    12841 ttaatattgt cagattcatt aaatggaaaa atatctgttg tttaaaataa ttaaaactag
    12901 aaaataaaaa tctcttagac gtattacata taaatttgta ttgcttaaaa tattaatata
    12961 aaaataaaat aaaattggag gaaatgaaaa tggttaaaga taaagttgta ttcgtaacag
    13021 gagctgcaag tggaataggt aaacaaatag ctgaatcgtt cttaaaaaat ggtgctaaag
    13081 taatgttctc tgatataaat caagaagctt taaataatac cataacagag ttacaaaaaa
    13141 aaggttatga ttgcatgagt gtaaagtgtg atgtaactaa ggaagaagaa attaaccacg
    13201 ctatagacaa aactgttgaa aagtatggca gattagatat attaataaac aatgctggac
    13261 ttcaacatgt ttctatgata gaagatttcc caactgataa atttgaattt atgataaaaa
    13321 taatgctgac agcacctttt atagctacta aaaaagcatt tcctataatg aaaaaacaag
    13381 gctttggtcg tataataaat atggcctcaa tcaatggtgt aataggattt gctggaaagg
    13441 ctgcttataa ctcagcaaaa catggactta ttggactaac aaaagttgct gctcttgaag
    13501 ctgccgattc tggtataaca gttaatgctt tatgccctgg atatgtagat actccacttg
    13561 tcagaggtca gttcaatgac ctttcaaaaa ctcgtaatat accattggaa aatgtcttag
    13621 aggaagtttt atacccactt gtaccacaaa aacgcctatt agatgttcaa gaaattgccg
    13681 actatgctat gttcttagca agtgataatg ctaagggtat tacaggacaa gcttgcttac
    13741 tagatggagg atatactgca caatagttat tatatttagg gcttgaagaa agtattcttc
    13801 aggtccttct actaaggagg gatatttatg agtattatcg cgccaattgg tataattatt
    13861 ggtatcatag ctattatata tttttcagtt aaagaaattc atataactat agctgctcca
    13921 atagcaactt taatagttgt tattttaaat aaaatggatg ttgtaacgtc aatgctaggt
    13981 gctgacaaaa acaattatat gggtgcactt ggaaactata taatgagtta ttttgcaata
    14041 ttcttattag gctcaatact tgctaaattt atggaagaaa gtggagctac tgtatcaata
    14101 gctgatttta ttttaaataa atttggctct aatcatcctt atagagtttt agttgctata
    14161 tttatagtaa gttttatatt aacgtatggt ggtataagct tatttgtagt tatgtttgcg
    14221 gtcattccac ttgcaagaac tctatttaaa aaacttgata tttcttggaa ccttattcag
    14281 ataccacttt ggcttggtat tgcaacaatt actatgacta taattcctgg aacaccagcc
    14341 attcaaaatg ttatacctat tcaatatcta aatacgtctc ttactgcagc tactatacca
    14401 agtattgtag gtagtatagg ttgtgctaca tttggacttg tctatatgaa atatgctcta
    14461 aacaaaagta ttagaaaagg tgaaacttat gcaacttaca cttcagaatt agaagaagaa
    14521 tctttagata gagaattacc aggatttttt gcaagtattt ttcctctatt agcactcgta
    14581 attatagcta taactggaag tacttttggt tcagaattta tgaaaaaaaa tgtaatttac
    14641 atagctttaa tagttgcaat tcttttatct gcttttttat tccataaatt tctatcatct
    14701 aagatacaga cattgagcat aggtgctagt ggttctatag gtcctatatt tgctacatct
    14761 tctgcagttg catttggagc agtaataatg gctgcacctg gttttgatgt atttagcaaa
    14821 cttataatgt caataccagg tagtccactt ataagtctta ctgttttgac ttcaacaatg
    14881 tcagcaataa cagggtcctc ttctggtgca ttaggaatag taatgccaaa ctttgcagat
    14941 tattatttaa gtactggtct aaatccagaa ttaattcacc gtgttgctac aatagcttca
    15001 aatatactaa ctatagttcc acaaagtggt gtttttctta cattcttaag cttatctaag
    15061 ttaacacata aaactggatt taaagaaact ttcattgtag ttgcagttgg ttcactaata
    15121 gcagaaatta ttgtaattac attaggatta ctaatgtatt aatataaaac aagttaaaat
    15181 tcataatatg aaaggtggtg tctcaaagtg aactttttac ttaatgagac actcttttta
    15241 tatgaaataa catatgccta tcatttaaac aataaaaaag gccttatctc tattttgaga
    15301 cagacccttt ttatttctta aagatttaaa taattataat ttgttgctaa tacgaaagca
    15361 tctactgaat aaatttaacc aaataaaaaa aaatttatca taactataaa tttacgttat
    15421 ggaattatta taaaaatatt attttctcta actaagtaat tccatactat acaggctttt
    15481 aatacaaatt gtcaaacaca ctgaaatata cctatttatt ccatatatat cttttttaca
    15541 tatctttaca gtaaaaaaag tcttaatctt atgttagtat agattcacgg gtaaccccct
    15601 acactaaagg ttacaaatca gtaacatttt tagaattttt agattaagag gtgtttaatg
    15661 atgagaagaa atataccaac aaaattaaaa actatattag ctttaggagt gttaacttta
    15721 gcaacagctt caagtgctgt tactgcaagt gcttcatcac ttgaaaataa tgtaaatata
    15781 agtaacaaaa gtttagtata tagtaatact aaatacaagt tttctgcaaa accaaattgt
    15841 tctgtaaaac cagaacttaa gcctgataaa gataactctt gtaaggataa gaatcataat
    15901 gacaagaatt gcaacaacac taataaacct gaggacaaca ataattcaga ctctactaat
    15961 aagcctgata acaataaacc tgagtctaat aaacctgaag acaacaataa ttcaggctct
    16021 actaataaac ctaataataa taaacctgag tctaataaac ctgaagacaa caataattca
    16081 ggctctactg ataaacctga taacaataaa cctgagtcta ataaacctga agacaacaat
    16141 aacaataatt cagactctac taacaaacct gatgataata acaactcagg ctctacaagt
    16201 gaaagttttt cagcttacca aaaagaagtt gttgacttag taaatataga aagagctaaa
    16261 gctggattaa atcctcttac tcttgattct tctatatcaa atgttgctac aaaaaaatct
    16321 caagatatga ttgataacaa ctatttttca cacaattctc caacttatgg ttctccattt
    16381 gacatgttga aaaaatttgg aataagctat aagacagcag gtgaaaatat agctatgggt
    16441 caaaaaactc ctaaagaagt tgtaaacgca tggatgaatt ctgaaggtca cagaaaaaat
    16501 ataatgaatc caaatttcag taaaataggt gttggagtag cacaaaaaag tggtggttca
    16561 atttattgga cacaaatctt tgtaggataa aatactcttt ttccatacat atcaaaagag
    16621 tatctctaag tgataaaaaa cacaaatgag atactctttt gtatctattt taaaattatc
    16681 aaatccaaat atacatacta taaatattat ttgttctctt aattttagtg tgtagaacaa
    16741 tatataaaaa gtattggcaa gatgaaaatg gaaaacttaa aaaagatatt taataggtaa
    16801 aaacaaaatc ttaatatctt cataaaaaaa ggctgtctca aaatagagat aaggcctttt
    16861 tcattgttga aatgattaaa atatatttga tttcatacaa aaagagtgcc tcatgaacta
    16921 aaaagctcac tttgagacac tctcttttat caatttatat aagcattata atactttaat
    16981 attattcagc taattttttg tatccttcat atctttcttt agcatcagct tcagctttag
    17041 caaataatcc atcagcttct tctgggaatt gtttagctat agcagaatat cttacttgtg
    17101 caagtatgaa atctttgaag cttgcagttg gttcttttga atctaaagtg aatgggtttt
    17161 taccttcagc ttttacttct ggattgaatc tgtataaatg ccagtatccg caagcaacag
    17221 cttgtgcttc gttagctaca gttcttccca taccttcttt taatccatgg tttatacatg
    17281 gagcataagc tattattaga gatggcccat catgtttttc agcttctata acggctttga
    17341 taaattggtt tttatcagca cccatagcaa cttgagctac atatacgttt ccatatgtca
    17401 tagccatcat tcctaaatct ttctttctag attttttacc tgaagcagca aacttagcca
    17461 tagcagcagc tggagtagct ttagaagctt gacctcctgt atttgaatat acttctgtat
    17521 caaatacaag tacgtttaca tcttcaccag atgcaagaac atggtctaat ccaccgtaac
    17581 cgatatcata tgcccaaccg tctccaccta gtatccattg agatctctta actagataat
    17641 cttttctatc ttttatagct tttactaact ctttagcttt ttcatctgta caatcacaag
    17701 ctggtttttc taataattca acaaccttag cacttgctac tttagaagct tctccatcat
    17761 ttctagaatc taaccactct gtaaatactg ctttagcatc ttcacagata cccatagaaa
    17821 ctaactctgt catagcatct actattttat ttcttatttg cttaacagct gtgtacatac
    17881 caaatccata ttcagcatta tcctcgaata aagagtttgc ccaagatgga cctttacctt
    17941 catggttaca agtataagga attgatggtg cagaagctcc ccatatagaa gaacatccag
    18001 tagcattagc tatcatcatt ctatctccat ataattgtgt aactacttta atgtatgcag
    18061 tttctccaca tccagcacat gctcctgaaa actccattaa tggttgtcta aattgacttc
    18121 ctttaacagt gtttacaggc ataacatcac cttttggagc tacctcatca ggatttactg
    18181 caaatgccca attatctaac tctgtatcaa gttgagtatc tattggcttc ataactaaag
    18241 ctttttcttt agctggacat atgtcagcac agtttccaca tcctgtacag tcaagtggac
    18301 taagttgcat tctatatttt aatccttcta atcctttacc taatgctttt ttagcttcaa
    18361 atccttctgg agcattagct aattcttctt cagtaactaa tactggtctt atacatgaat
    18421 gaggacatat aaaagaacat tggttacatt gaatacagtt atctattatc cattcaggaa
    18481 catcaacagc tataccacgt ttttcataag ctgcagtacc acaagggaat gttccatctt
    18541 ctataccatt gaatgtactt actggtaagt tgtttccttc ttgtgcagtc attggtctta
    18601 gtatattttt tataaattct ggttctttaa cttctccttt atctgcatca acagcatttt
    18661 cccaagaagc tggaacttca actttaacta aagcatttat tcctgcatca acagcagcat
    18721 agttcatgtt aactattttt tcaccttttt taccataagc tttaactata gaatctttta
    18781 agtatttaac agcatcttct tctggtatta tttcagctaa tttaaagaat gcagattgca
    18841 ttatcatatt tattctgttt ccaagaccta tttcttgtcc tagtttaaca gcgttaactg
    18901 tatagaattt tatatcattt ttagctatat attgcttcat atgagctggt aaatgttctt
    18961 caagtcctgc ttggtcccat atagtgttaa gtacaaatgt accacctttt ttaagacctt
    19021 ttaataaatc atattgataa acatatgatt gtttatgaca ggcaatataa tcagcttcat
    19081 ctattaaata agtagctctt attggagtat caccaaatct taagtgagac atagttatac
    19141 caccagattt tttagagtca taatcaaagt aagcttgagc atatttttca gtatggtcac
    19201 caattatttt gatagcttgt ttattagctc caacagtacc gtcagaacct aatccccaga
    19261 acttacatct tatagttcct ttagaagcta tttttattgg ctcagatgga gttaaagaag
    19321 tattagttac atcatcaact atacctactg taaatccatt tttaggttgt tctgaaacta
    19381 agttatcaaa aacagttttt atatctgaag gagtagtatc ttttgaacct aatccgtatc
    19441 ttccacctat tatcatagga gcattttctt ttccatagaa tacatcacgt acatctaagt
    19501 ataatggttc accagttgaa cctggctctt tagttctatc aagtacacat attctttcaa
    19561 cagtagatgg catagcatct aagaaatgtt tcattgagaa tggtctatat aaatgaactt
    19621 taactaaacc atatttttct cccttagcat ttaagtaatc tatagtttct tctattgttt
    19681 ctgttactga acccatagct attaatacgt atttagcatc ttctgctcca tagtaatcaa
    19741 ataaactatg ttttctacct gttaaattac tcattttttc catgtatttt tcaactatac
    19801 ctactatatt ttgatagtat ttattagaag cttctctagt ttggaaatat atatctgggt
    19861 tttgagcagt accacgagca actggatgat ttggagataa agcattatct ctaaatgctt
    19921 gaactgcttc aaagtttaat aaacttgcat aatcttcatt ttctagtaat tcaacttttt
    19981 ggatttcatg tgaagttctg aatccatcaa agaaatgtat aaatggtaat ctaccttcta
    20041 ttgcagctaa atgtgcaaca ggtgctatat cagctacttc ttgaactgaa ccagaagcta
    20101 ataatacaca tccagtttgt ctagcagcca taacgtcttg gtgatcccca aatattgata
    20161 acgcttgaga tgctaaagca cgagcactta catggaatac tcctggtaat aactctcctg
    20221 caactttata catgttaggt atcattaata ataaaccttg agatgctgta aaagtagaag
    20281 ttaaagctcc tccttgtaaa gaaccgtgga atgttcctga tgcacctgct tctgactgca
    20341 tttcaacaac attaactgtt tgtccaaata tattttttct tccttgtgat gcccactcat
    20401 caactacttc agccatagtt gaagatggtg tgattggata tatagctgct acatctgtaa
    20461 acgcataggc aacatgagct gcagctgtat ttccatcaag tgttttcata aacttagcca
    20521 taatatgtat cctccttgat agctacaatt gtacttgttt ataacaagtc tattttttta
    20581 tatttacata taatttacac aacaaaacta taaacacaca tatagtcgcg caaatactct
    20641 atcttctaat ataatactac agattagata aaattccttt gaaaattgta aattaattac
    20701 atatttaagt agattttttt taaaattaaa tatgttaagt ttttttcata attttgtata
    20761 caatatataa taaattaagt tagatttttc aaactttttt atctatttgt tcatattagt
    20821 ataaaaagta aaat
//
LOCUS       DAMJYX010000064        19862 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJYX010000064 DAMJYX010000000
VERSION     DAMJYX010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.064"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            237..1664
                     /locus_tag="PC479_002419"
     CDS             237..1664
                     /locus_tag="PC479_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2804958.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(1741..2010)
                     /locus_tag="PC479_002420"
     CDS             complement(1741..2010)
                     /locus_tag="PC479_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804959.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(2013..3455)
                     /gene="nhaC"
                     /locus_tag="PC479_002421"
     CDS             complement(2013..3455)
                     /gene="nhaC"
                     /locus_tag="PC479_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2804960.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(3480..4646)
                     /locus_tag="PC479_002422"
     CDS             complement(3480..4646)
                     /locus_tag="PC479_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2804961.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(4871..6991)
                     /locus_tag="PC479_002423"
     CDS             complement(4871..6991)
                     /locus_tag="PC479_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2804962.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            7224..7625
                     /locus_tag="PC479_002424"
     CDS             7224..7625
                     /locus_tag="PC479_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804963.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            7668..7961
                     /locus_tag="PC479_002425"
     CDS             7668..7961
                     /locus_tag="PC479_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2804964.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            7976..8416
                     /locus_tag="PC479_002426"
     CDS             7976..8416
                     /locus_tag="PC479_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2804965.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(8508..8981)
                     /locus_tag="PC479_002427"
     CDS             complement(8508..8981)
                     /locus_tag="PC479_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2804966.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(8981..9769)
                     /locus_tag="PC479_002428"
     CDS             complement(8981..9769)
                     /locus_tag="PC479_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2804967.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10097..11341
                     /locus_tag="PC479_002429"
     CDS             10097..11341
                     /locus_tag="PC479_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2804968.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            11664..12761
                     /locus_tag="PC479_002430"
     CDS             11664..12761
                     /locus_tag="PC479_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2804969.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            12752..13450
                     /locus_tag="PC479_002431"
     CDS             12752..13450
                     /locus_tag="PC479_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2804970.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            13453..14517
                     /locus_tag="PC479_002432"
     CDS             13453..14517
                     /locus_tag="PC479_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2804971.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            14700..15404
                     /locus_tag="PC479_002433"
     CDS             14700..15404
                     /locus_tag="PC479_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2804972.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            15397..16719
                     /locus_tag="PC479_002434"
     CDS             15397..16719
                     /locus_tag="PC479_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2804973.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            16906..18279
                     /locus_tag="PC479_002435"
     CDS             16906..18279
                     /locus_tag="PC479_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804974.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(18651..19589)
                     /gene="pdaA"
                     /locus_tag="PC479_002436"
     CDS             complement(18651..19589)
                     /gene="pdaA"
                     /locus_tag="PC479_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2804975.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         7345 a         2498 c         3091 g         6928 t
ORIGIN      
        1 tttgtacata atctaattaa aaaaagtaac tttaaatggt taactaagga aatattgtta
       61 caaactttag ttatataaaa aattcgtttg atgagcaaag tattaatgtc aagtaagaat
      121 aaaagcttat tagataagaa aaataatatt ttataataat gatgttactt aaattattat
      181 gttatactat tttaaatagg aaacatttag atactataat tttaggggga gtcaagatga
      241 aattaactaa atcaatatca tcaatgctgt gcttatctct agttttaagc ttatcaccag
      301 tagcatatgc aaatgaaata agtagaacaa cagttgagaa tactttagta ggtcaggata
      361 gatatcaaac tgctgttgaa gtaagtaaaa aaggatggag tagtgcaaat gaggctgtag
      421 ttgtaaatag tgaatctatt gtagatgcat tagctgtaac accatttgct aaattaaaaa
      481 atgctcctat attattgaca gaaaaaaata atctaaattc acaaactgag aaagaattaa
      541 aaagattagg agttaaaaaa gtttatataa ttggtctatc tagttctgta tcaaatgatg
      601 tgcaaaaaca gttagaatct aataatttaa ctactgatag agttggtggt agtgatagat
      661 accaaacagc tttgagtata gctaaaaaaa tagaaggatt aaaagatata tcagaaatag
      721 ctgtagtaaa tgggtacaca ggtcttgcag atgcagttag tatagcttct gtagctgcta
      781 ctaatggaat ggttatactt ccagtatctg atgcatctgg tatatctagc tttaaagatt
      841 ttatagaatc taagaatata aataaatcat acataatagg ctctactaat gctgtatcag
      901 ataaaataaa acaaagttta cctaattcag aaagaatagg tggagcagat agaaatgaaa
      961 ctaatggtaa agtaatagaa aagttttata catctaataa attaaacaat gtatttgtgg
     1021 ctaaggatgg aatgaaaaat caaaatcaac ttatagacgc tttagcagta ggagtactag
     1081 catcaaaaga aaattctcct gtactaatcg taggagaaca attaagtgca acacaaaaga
     1141 gtgtattttc gaataaaaat gctacctcta taactcaagt tggtagtgga ggaaatgaaa
     1201 atgcatttaa agaattaaaa gatttacagg caaatagtaa caataagaga gtaatgtatg
     1261 taactaatac agataaggta aatataagaa atgatgctac aatagaagct tctgttatag
     1321 gatatctaaa taatggtgat gaagtagaag ttttagatgt gttaaaaact ggatgggtag
     1381 aaataaaata caatgaaggt attggatatg ttagtgggtc ttatttgaca aataataagc
     1441 ctgataatag taatgaaaat ataaaaataa agtatgtaaa agaaaaagat ggtctaaatg
     1501 ttaggaaagg accaagtaca gaagatgaaa aaataggtca cctttcatat ggaagcaaag
     1561 tagaaacaat agaaatgttt gctactggat gggtaaaaat aaagtataat ggtggatatg
     1621 gttatgtaag taatgattac ttatctgata caccagttat ttaattataa gtaacaataa
     1681 aaaaactgct ttaatttgta aagcagtttt tttataaaaa tttaagtcta ataactattt
     1741 ctattttgat accttaatcc tgggatttat tattatggat attatcaaca tacaaacact
     1801 ccaaatccag tgtctaattc caaacttaga tattaataaa ttcagtcctg ctatggtaaa
     1861 taagcagtat ttaattacaa ttaaaagtgt atttatagaa agccccttca tagaaagtat
     1921 cctaaatcca ataatagaca ttctaagtcc tataaatgcc aataatatac ctgcaatatc
     1981 ctgaattaag taatagtatt caaatttcat attcaatcaa ctcttctctg agcagtatca
     2041 aatatttcta actcttcaat ttcatcatct gtgtatgtaa gcatagtaaa accagttaca
     2101 ccataaataa atgaaattat aggtgtaatc caacttaaga aacaatatgg aataaattct
     2161 agtgggctta caccaagagt ctgagcacag aaaacagcat ttgaattcca tggtattaat
     2221 ggaccaccaa gtgtagctgt atcttctata attctagata gattttgtgg ttttaattta
     2281 aattctttgt atattggaga cattagagtt ccgacaaata cagaagtgaa catcattgat
     2341 gaactaaatg catttgcaaa gtaacttact ataaaagtag acccaaccaa tttagttctt
     2401 gattttccta ttttagttat aagaggttga agcagtactt ctaagaatcc agttttttgg
     2461 agaataccag caataacgaa aactacaaat actagtaaaa cggtttcagc catactcatt
     2521 attccaccac gatttaatag tttgtcggca tagacaaatc cagtatctat agtaaatcct
     2581 gatagcatgt aatttaaaac atcacctatt ttttctcctt cttgaaatac tgctataata
     2641 agtcccatga tagctgaact tagtatagat ataatgggtg gcttttgtag caataaaagt
     2701 aataataaaa atattattgg aatcattgct actaaaccaa tatgaaagtt tgaatttaat
     2761 acatccttga tttgatttat ttgaacatag tctatagtat tatttgaata cttaaatcca
     2821 attactgtat acagaactat acatattacg tatgcaggtc ctgtggtata aaacatatgc
     2881 ttcatatgtg taattacatc tgtcttacaa actgctgcag ctaagtttgt agaatctgat
     2941 agaggtgaca tcttatctcc aaagaaagca ccagaaatga ctgcaccagc tgttaaacca
     3001 agaggaatac ccatactagt accaatactc atcattgcta taccagaagt tccaagagtc
     3061 ccccaagatg taccagtagc aacagaagtt aatgaacata cgattaatgt tgtaagcaga
     3121 aaatatttag gggttattat tgttagtcca ctatatacta ctgttggtat tgtaccagca
     3181 gctatccaag ctgctattag tacaccaaca gaaagtataa ttaagtttga ttgaaaggca
     3241 tctttaccaa cttcaaatcc aaaagcttct atttcatcat tagtataacc aagtttcata
     3301 gctgcaggaa ctactataag ccatgaaagt aaaaacattg tagttataga agcattaaat
     3361 acaactatac caactgatgt tagagctatt attgcaagca ttactaacaa ggaatatcta
     3421 aatgaaggta tttttctttc tgtattcatt ttcatagtgc aaattctcct taaatatttt
     3481 tagttaattg cattttttat tcttataaga gcttcttcaa gcatacttct tggacaagct
     3541 aagtttattc tttgataccc acttcctcca atgccaaagc tgtttccttg atttagagca
     3601 actttacctt tttgtacaag tatactttct agttcctcat cacttaatcc aagtgcacta
     3661 aagtctaccc ataataaata agtaccttct ggttttctaa ctttaagttt tggcatattt
     3721 tcatttatat attttatagc aaaatctata ttagattcta aatattctaa aaatgattct
     3781 agccaagatt caccattatt atatgaagct tcagttgcaa ctaagctaaa gcaattattt
     3841 ctctttatat caattctggt aaaggcatca tctaacaatt tatagtcttt ttcatcaggt
     3901 agtattacat atgatgattg aagaccagct atattaaaag ttttagttgg agccatacaa
     3961 gtaattgtat ttttttcaaa ttctttagaa atagatgcca taggaatatg cttatgtttt
     4021 tttaatataa tgtcactatg tatttcatca gatatgattt ttacattatg cttaagacaa
     4081 atatctccta gtttttttaa ttcatctttt gtccatactc taccaactgg attatgagga
     4141 ttacaaagta tgaataattt tacatcttta atcttgtttt caatgtcttc ataatccatt
     4201 atgtagtttc cattttctag tttttgtaat gggcttataa ttagttctct attgttattt
     4261 tttacaacac tattaaaagg gctgtaaact ggttcttgta tcattatttt atcatttgct
     4321 tttgtgagtt catttattaa aagacttata gctgggataa cacctggact ataaattaac
     4381 cactcacttt ttattttcca gttatgtctc ctatctagcc agtttactat agattcattg
     4441 taagaatctg gtcttgttgt gtagccataa atctcttgtt ctagtctatt ttttaaggaa
     4501 tcaattatac aaggtgcagc cttaaaatcc atgtcagcca cccacatagg aagtaaatca
     4561 ttagttccat actttttttc catttctgac catttagatg aaaaattatt gcttctatca
     4621 acaatctcat taaaattata attcattgta aatacctcca gtaattttaa ttttatgtta
     4681 ataatattaa gcaaacaacg tgccaatatt gataataaaa aatatttatt cacaagttaa
     4741 tatttaatat taagtaattt cagagcatag ataagataaa aatattaatt atacttaaaa
     4801 ctttgtggaa aaggttattt ctaataaatt tttaaatata tgaaaatgta attaaatagg
     4861 ttaaataggt ttaactgttt aacttgttag acctatttat attttttaga aattctactc
     4921 cttttgatgt tataattgaa cctccacgac caacaagtat gtctacaagc ccaaagtttt
     4981 gtagttctaa aagcatattt ctaatctgtt gttctgtcaa aaatctattt tcctctaaag
     5041 ctattttaca taagcttttt ctacctattc tttcttttaa atcataagcc ttttttaaat
     5101 tatccaatat aaatatgtat tcatctagtg accttttaaa attatagtcg tatttcatga
     5161 tgtttttgtc tttgccaatg ttgaattgat acttttttat attatcactt actttattac
     5221 aaacggttct actataattt gaatctatag tatctaatat atattcaggt aaatcacata
     5281 tttctataat atctttttcc aagtaacaga agtattctcc taagtttctt aattctctta
     5341 catttccttc ccatctatac attttaaaga tttcttttac ttcctcactt agaataaaat
     5401 tacatggaat atcatatttt aatgaaccaa aaatttcaaa tatatcagca ggtctttccc
     5461 taagtggatg tattttaagg ggaagaacat tcaacctaaa atataaatct cttctaaaca
     5521 tatttttaga tacaagttct tttaaatttc tattagtagc tgcaattatt cttacatcaa
     5581 tgtcaatgac attattagaa ccaatacgtc gtacttgttt ttcttgtatg actctaagta
     5641 atttagattg agaatttaaa tccatttctc caatttcgtc taaaaatata gttccattgt
     5701 tggctaattc aaataaacca attttcccac cttttttagc accagtaaaa gccccttcat
     5761 catagccaaa taattcactt tgcagtagat tttcttgaaa agtactacaa tttactgcga
     5821 caaaaggacc atcttttctc ctagaagcac tatgaatgga ttgagcaaat aattctttac
     5881 cagtaccact ttcaccaatt ataaggatag atgaatttga ttgagccatt ttattagcaa
     5941 ttttttttgt attaatagtt tgtatactag aacctaatat gtcatcaaat gtgtatttac
     6001 tgatattacc agagtttact agttgtgctc ttaatttagc ttgctttttt tctaattgag
     6061 aaaactttgt catctttagt ataaatccat caaatacatt taattgtata tgtttaatct
     6121 ctaaatttat atcaatatga tttattttaa tcaatttctc aaaaaatgga gttttattgg
     6181 tcaatatgct ttgaaaattg atgtctttta tgcaatcacc aacaaatgag tcaatcattt
     6241 catttgcatt tatagacaaa atttttctag ccatatgatt atagaattga attattccat
     6301 cgttatttgt gcatataatt ccatcatcaa ttatagatag caacaaatca aattgtcttt
     6361 caaacttatt tgcatctaac aataactcac ctgtactata acttgtagga attatttttt
     6421 ctacgtattt ttttattagg tcatctttta ttagatgttt taatttggtt tttgtagcaa
     6481 tttcaacaat acaacttaaa tctattattc tgtattttaa atctataact tcacaatttt
     6541 taaacttagg caagattttt tcgccaggag ttaggattac agcatctgtg ggagtaaact
     6601 caatctctgg atagcagggc aggatattta tattgtctat tcctaatcta tgtattaaag
     6661 aaattgtttc tattgccata tctttactta agttatatac ataaataatt ttatcttttt
     6721 ctaatttgga tatcatatca atactgttct tttcaaatgt caacttagga acaatgattt
     6781 gagcatcata gtgagataat tttactattt catcatattt taaataagca gataggagta
     6841 taagtttttc tttaaaaaac tttaaatttc cctcttcaaa tgagtataaa tttatcttta
     6901 gattatcacc taagaattgc cttatttgat tttcataaat ttctccagcg tcttttttta
     6961 aacttacaat agctaattcc atttgtttca ttttaacctc tcatacaaat ttgacttatt
     7021 ttagattaca taataattaa attagattaa ataatattat ttaattatta ttataaattt
     7081 attatagact acacattgaa gtaaaagaag tagctaaaat tattcctttt atttttgttt
     7141 tttgtatcta tttggtataa tagtattaga gcgtataata atttaggcaa aattttttaa
     7201 ggaagactag aggagaatat aaaatgaaaa aatgtcttta ctgtaatagg gatataaagt
     7261 caccaaataa taaattagct ataaaatatg atgacaatat gaatatatat ccatgtcgtg
     7321 cagaatgtaa ggaaaaaatt gaagaatata tagcaaaaaa acctacatat aagtttataa
     7381 atatattaga ggttctttta ggtgtgggcg gaatattctg ctttttgagt aaatggacta
     7441 ttgcagccca agttgtactt ggaattgatg cactggttat atttttattc ccattggcag
     7501 gatttaaaat gaatggaaag ttaagtatgg aacaaactaa gaatcaaagt aggtctattg
     7561 caatatcaat tttggcattt atagtaataa taactgtatt atattttaaa caagttggta
     7621 agtagagcat aattgtataa gacatcatta gagtatgagg tgagtttatg gaagaaagaa
     7681 aattagcacc aaacaatgag agggaagctc tcataaaaag attaaaaaga atagaaggtc
     7741 aagttaaggg tatacaaaag atggtggaac aagaacgtta ttgtgttgat atattggttc
     7801 aaatatctgc tataaggtct gctataaata gagtaggcac aattatctta gagaatcata
     7861 taaaaggatg tgtagcagaa agtattaaac atgatgatgc tgataaaact gaagagatga
     7921 taaaagagct tatggatact ataaataaat ttacaaaata aggaggcggt tagttatgaa
     7981 tcaacaagga tttattatgg aaattgttga tgatagaact gcaaagttaa aaatgcaaag
     8041 acattcagct tgtgctgctt gtggaaaatg tgctactaca aattcagcag agtcaaaaga
     8101 aatattagta gaagtagaca acacaattgg tgcaaaagtt ggtgaccatg tagaagtgag
     8161 tatggataat atgaatgtat taaaagcagc tgtaatggct tatataatac ctcttatagc
     8221 acttcttgta ggaacaatag ggacttatta tatattgggg gcaattggaa tgacaactgg
     8281 agttgaagcc ataagtggat tagttggaat ttgtcttaca ataatctgtt atttatactt
     8341 aaagaagaat gacaaaaaat ttagagacag tagagaattt ataccagtaa taacaagaat
     8401 attaattgac ctttaagata attataatta aaaaatagca atgtactttt taaataaata
     8461 ctcaatacgt atttgtagag tttaagtaca ttgctatttt tatattttta ttttgatttt
     8521 ctgtaaggaa taaagttttt tctgattttt aaaagattca atgttcttac aaaagaagtt
     8581 acaactaata caccaagtgc tattgcagca cttagaaata gcgcatctgt accttttgac
     8641 atagccaaag aataattttt ctgaatcaaa gacaacattg tattatatag cccaattcct
     8701 ggtattagag gaagaatacc tggaattacg aacacaatag ctggttgctt tagttttctt
     8761 gctaaagtct cactgcacgc tgagaccaaa gcagcagcga taaaacctgc aaaaactgca
     8821 tttgtagtaa aattaaataa ataaacatat acaatccaac caattccgcc agttactcca
     8881 gctggaagaa gtagatatag tggagcatta aagaaaacag caaatccagc agttgcaaca
     8941 cctgaaaata aaaaatgcaa aatcatagac atatcagtca ttagaaagga cctcctattc
     9001 tataccatgt atcaaggaca aaaccaactc cagaagcaat tgcaataata atcatagcag
     9061 catcaaaagc acgagaaatt cctgatatta aattccctga tattaaatct ctaatagctt
     9121 ttataaaagc aactcctggg agtagtggca ttatagcgcc gactataagc attttagggt
     9181 catctaaaat gcctatttca actagtagaa ctcctgaaag tgcaattaaa aaagaagata
     9241 ctaaacagga aaatgcagat attgcactta tcttcatcgt tttatcataa gtaattgcag
     9301 caaagattgc tattataaac gtaagtataa aatttaatac ataattccct ccaagcaaac
     9361 aagcaaaaca agcagatgca attcctgtac aagagtaaaa taaccataaa ggataaggtt
     9421 ttacatcttg gatttcataa agacgtgcaa tggcattatc aatatcatac tctttgtcac
     9481 taacaaattc tcgagaaaaa ctgtttaata aagaaatctt atttaagttt atacctcttg
     9541 attgaatcgt tttcataaac gaaaagccat caaatttctc atctgaaata attattactg
     9601 taggagttgt aaaaatattt acatgataaa accctcttga cctgcatatt ctaaggacac
     9661 tgtcttcaac cctagatgtt tcagctccat tttttatcat cagttcccct acaaatagag
     9721 ctagttttag aatatttttc ttatattcaa gttcctcatt ttgattcata aatacacctc
     9781 caaattaaaa aacgtaatat tgaaattata tcaaattttt aagtaatatt ccttaatatc
     9841 tatacaaata tattatcttt tttttgtttc aaaatagtca catttaaatt atatccaatt
     9901 aaaagaatta gacaatatta tttattatat tttaacttaa aaataaagaa agtaactatg
     9961 ttaaatatgg aaaaatggca aaacacttga ctattaattg gtaaaggact ataataataa
    10021 attatcatat tgataaaaac gaaaattatg aaatgtctgt aaaaataaaa tttaaataat
    10081 aactaggagg agtaatatgt ttgagaactt gaaaaaagcg gaccctgaga tttatgaaag
    10141 tatgaaaaga gagttaaaaa gacaacaaag aaatattgaa ttaatagcat cagaaaacat
    10201 agtatcagtt ccagtaatgg aaactatggg aagtcatctt acaaataagt atgcagaagg
    10261 ttactcaggc aaaagatatt atggtggatg tgaatacata gatgaagttg aaactcttgc
    10321 aatagataga atcaaaaaaa tatttggagc agaacatgct aatgttcaac ctcactctgg
    10381 agcaaatgca aatattggtg tatattttgc tatgttaaaa ccaggtgatg ttgttatggg
    10441 aatgaattta tctcaaggtg gacatttaac tcatggtgct ccagttaata tttctggtca
    10501 atattataaa ttttatgaat atggtataga taaagacagt ggacttatag attttgatga
    10561 agttagaaag atagcacatg aagtaaaacc taaaatgata gttgcaggag caagtgcata
    10621 tcctagagaa atagatttta aaaagtttag agagatagct gatgaggttg gagcattgct
    10681 tatggtagat atggctcata tagcaggtct tgtggctgct ggtcttcacc aaaatccatg
    10741 tgaagtggct gattttgtta caacaacaac tcacaaaact ttaagaggac ctcgaggggg
    10801 tgttattctt tgtaaagaaa aatatgctaa agacatagac aaagcaatat tccctggtat
    10861 tcaaggtgga cctcttgaac atataattgc ttctaaggca gtttgcttta aagaagcttt
    10921 aagtgatgag tttaaagaat atcaggtaca agttgctaaa aatgcaaaag ccttagctga
    10981 agaacttatt aaaagagatt ttaaattgat ttctggaggt acagataatc atttaatact
    11041 attagatttg actaataaaa atataactgg taaagcagca gaaaagagat tggatgatgc
    11101 ttatataaca gctaataaaa atacaattcc atttgaccca aatggtgctc ttattacaag
    11161 tggaataaga cttggtactc cagctgtaac tactagaggt atgaaagaag aagatatggc
    11221 aataatcgct gaggctatag acctatgttt aacttatgat gaagaatcta aggctagaac
    11281 attggttgtt ggtttaactg aaaaatatcc tttatatgaa gaatataata ttatggatta
    11341 attataaaga acaactaaat aaaatacatt taaataatta caatgattgt gtatgtattt
    11401 gaatatagtt agatataaaa aaggtgtctc aaaatgaact ttttagttta tgaggcactt
    11461 ttttgtatgg aatcgaacat atttgtgtta tttaaataat aaaaaatatg gttatctcta
    11521 tttttagatg gtctctttgt attttttaat taaaacttta agatttctta agaattatac
    11581 taggtttttc taaagattta aagtctatac ttataagtat agtaatcata tgaaaagaaa
    11641 agctttaacg aggagggaat cttatgaagg tattaattct tacaggaaaa tttggtatgg
    11701 gtcattactc agcatcaaat tctttaagtg aagatataaa agcaaaattt gataattcag
    11761 aaataataat taaagatata tttgagtaca ttatgcctaa ctattcagat aaaatgtaca
    11821 agactttttc tattcttgta aatcggggaa gtagtcttta taatttattt tataaatgtg
    11881 ctgaaaatgg aaagaaagat ataaaattta ctttttcaga ttatttttta aataaattag
    11941 atacattact tcatgaagta caaccaacag tagttatttc aacttttcca ttttgttctc
    12001 aattagtatc aagatataag gaaaaatata actcaaatct tcctttaata acatgtataa
    12061 ctgatataag tagtcactca gaatggatta gtaaaaatac agactgttat ttagtagcta
    12121 gtaaatctac taaggaagaa cttgttttta aaggaataga tgaatctaag attaaagtta
    12181 atggtattcc agtaaaaaaa gaatttaaaa gaatagaaca tgtaaatcat tcaactaaaa
    12241 aaaatattct tataatgggt ggaggattag gattactgcc taaatcagaa caattttaca
    12301 aagaattaaa tagtttagaa ggagtaaaaa ctactgtaat aactggtaat aataaaaaaa
    12361 tgtactataa actatatggt agatatgaaa atatagaggt agtaggttac accaatgaag
    12421 tatataaata tatgaaagat tctgacctta ttatttctaa accaggtgga ataacactat
    12481 ttgaaacaat ttattcagaa cttccaatat tggcatttaa tccattttta caacaagaga
    12541 tagataatgc tagttttata ttaaataatg aaattgggag aatacttggt aaaaataaaa
    12601 aatactatgt agatgagata aaagatttaa tttatgatga tgctactctt aaagaaatga
    12661 gcagtaacat gaaagaatta aaaaaacaat ttgataataa taccttagag aatattttat
    12721 tttcacttga tgaacaagga gcgtgtagag aatgtatgta gtaggtttaa ttatcgttgt
    12781 tgcattgatt tttttagtcc attcaattat acctacatac tataataaac tattaaataa
    12841 agaggtatta aaaaatatgg ctggagaaaa tgaaattgca cttacatttg atgatggtcc
    12901 agataagaga tatacagaaa aattactaga tgtgttaaaa gaaaatgata tacaagctat
    12961 gttttttgta gtagcaaaga atgctgaaaa agagcctgaa attataaaaa gaatgttgag
    13021 agaaaatcat atagttggtt tacattcatt ggaacacaga aatgcatggc tttatagtta
    13081 tagttatgtt aaaaaagatt ttattgaaag tacaaatata atgaaaaatt taggtgtaga
    13141 tgtaaactac tatcgtccac catggggtca tactaatata ttttcaaatt catttgttaa
    13201 aaaatataat ctaaaaatga ctttgtggga tgtaatggca gaagattggg agaaagatag
    13261 tacagtagac attattataa acaagttaat gagtagaaca aaagaaaatt ctataatttg
    13321 tttacatgat gcaggtgaaa attcaggtgg tgcagtaggt gcacctgagc gtactattga
    13381 agctttaaag atagctattc caaaacttaa agccagtggt ttaaagttcg taacaccaga
    13441 aaggatgtag ttatgataag taaaactgaa aagaaaaata aatgcctagg tagtgtagct
    13501 tttttagttt tactaatggg cataacagga tattttgttt ttagagggca gtcagttgaa
    13561 tcattgataa aatcattaaa aggtgcaagc cctatgttta tactaattgg atttgcaatg
    13621 atgtttatat atgttgcctg tgaaggtata aatatatact taggtatgaa agctttaaac
    13681 caaaagacta ctcttttaaa atgtatggga tatgctttta ttggatttta ttttagttct
    13741 attacacctt cagcatcagg tggtcaacca gcacaagttt attatatgaa aaaagacgat
    13801 ataaatatat catattcttc acttatacta ttagtcattg ttgttattca ccaagtagtt
    13861 atactggcat atagtggaat aatgttcatt atggaacgtg agtttatatt aaataatgta
    13921 agtggtatga acattttact aatatatggt gtaataacaa atgttgctct tgtcattgga
    13981 gtaattgcaa ttatattttc aaaaaaactt gtaaacaatt ttataatatc aataacaaat
    14041 ttattaggta agttaagaat aataaaagat gttgaaagtt caagaaaagt tataatatct
    14101 caaattgaag agtatgttaa aggtgctcaa tatataaagc agaatccaaa acttgtagtc
    14161 caaattttag tcataactat tgtacaaata acagctatgt ttttagttcc tttctttgta
    14221 tacaaagctt ttcatctatc aacatatact gtatttgaga tattagcgat acaatccttg
    14281 cttaatattg ctgtttcttc attaccattg ccaggggctg ttggagcttc tgaaaatagt
    14341 tttatgacat tatttaagat atttttccct ggtcacttat tagttccggc aatgttactt
    14401 agtaggggaa taagttttta tgcatttgta gctataagtg gattgatatg tatagtagta
    14461 catgtaaaat cttctgaaaa agtaaaagca gtgaaaaaag ttgcttatgt aagataatat
    14521 tatatagatt atttttatta atatgtcaat ttgtgtgtat attttaatta agctttaaac
    14581 atatttacag gagtagaaac tattaaaacg tgtgacattg ctaagctttt gctaataaag
    14641 tataatattt tatatagttt gaaatattta agaaatatag caaaaaaagg agcttggtga
    14701 tgaataaaga gattccaaaa aaaattttag tagctgatga taatcctgag attagggaaa
    14761 tcgtagaaat acttttaact ggtgaaggtt atgaagttat tatggcaacg aatggagaag
    14821 aagctgtaaa cttagtagat agtacgattg atttgattat tttggatgtt gtaatgcctg
    14881 taaaaacagg atttgttgca tgtaatgaaa ttagagaaaa aacgacagca ccaatattat
    14941 ttttaactgc aaaaactcaa gactctgata aagttattgg attttcagca ggaggagatg
    15001 actatttatc aaagcctttt tcatattctg aactaatttc aagagttaag tctctactta
    15061 gacgttatta tatataccag ggaaaggaaa aggaaagaga gccaaatata ttaaaaacaa
    15121 aagagttatg tgtcaattta gatactcaag atgttacact aaatggagaa cctgtgtttt
    15181 tgacaactat agaatatagt atattaattc ttatgttgaa aaatagaaaa aaagtttttt
    15241 cgtctgaaaa tttatatgaa actatttggg atgagcagta tttttataca gcaaataata
    15301 caataatggt tcatattaga aatttaagaa aaaagttaga aaaagaccaa aaaaatccac
    15361 aatatataaa aaccgcatgg ggaaaggggt attatattga ttaacaaaat taagccatct
    15421 aagttgggag ttatactatt gatatatgta gtaatctcag ctgcagtggc tttcatttta
    15481 aatataccat tttactctgc tgttgaatct atagtaaaag atgtatcaga agggaaagta
    15541 tattttaaaa gagaagtaaa tcaacttatg gataattttc aaacttatgt gaataacaat
    15601 ggtataagag cagatgatgg agatgatata gcaaaatgga atagtgataa ttggtttgtt
    15661 tctatgaaga tatataaagg aaataaggtt ttttatgata cattatacta tcctaatgaa
    15721 gtacctgata agagtgagga aaatgagttt tatagcagtc ccatggctag aaactatgag
    15781 atacaatttt ctgatattaa agctaatata gttgtagtag ctttatttag atttagattt
    15841 caaaatttta tagaagcaat ttctattaca tttatggcac ttacatttat tataacattt
    15901 ttactgttgt ttagtagaaa gataaagtac ctaaggaaaa tagaaatggg tataaagata
    15961 gtagagagtg gcagtttaaa gtatagaata ccagtaaaag gtaatgatga attatcatca
    16021 cttgcatcta gtattaatga gatgagtcat acattggaga atcaacttat aaaagaaagg
    16081 gagatgaaga ataaaaataa agatatagta acttcaatat cacatgatgt tagaactcca
    16141 ttaacgtcag taatttgtta tttagatttg attaaagacc ataaatacaa tagtcctgag
    16201 caattggaac aatacataaa aaatgtaagg tctaaagcat atcaaataaa agatttaaca
    16261 gatgaccttt ttacgcattt tgttaattct ggagaggaag tttcaaaagg gtatgaaaaa
    16321 ataaatgcta atgaatttat ttttcaatta cttttagata gttcatatac attggaggag
    16381 aaaggatttc atataacaat agaaaatgaa ctttataacc aatatcatat ttatgtagat
    16441 gttacacaat ttagaaggat atttgataat ttatgttcta atataataaa atatgctagg
    16501 aaagataagc ctatagtatt tttaattaaa gtagaggatt ctaaattact aattatacaa
    16561 aaaaatagag taagtaattt ttctaagaat gtagaaagtt ttggaattgg tataaagaat
    16621 tgtgagaaaa tagttgcata tcataatgga agcataaatg taaattcaga tgataaagaa
    16681 tttgctattg aaatatctat tccagcaaaa gaatcataaa aatttaaatt atatattgtg
    16741 taattgctat tatatattgt gtaattgctt atgtaaaatt ctatgaagca ggaagcatgt
    16801 gactttagtt atgggatgtt caatattgtg tacctctagg caagtaacaa gtttacttac
    16861 aagaccatat aatattatta tcttaaaaaa aggggacata ataatatggt tttgacagga
    16921 gcacattata tttacattct atttatggta attatattaa ttactatgat tatgaaaaag
    16981 gatacaatag taccatgtat attaggtgtt ttttttatgg ggttgttctt tgaaaaaaac
    17041 atttttggtg ctataacagc agtgtttaac tcttttatta tatctttaaa tgagttgggt
    17101 ccaattatct tgattatagc tattatggtt gcattatcta aagccctaga agccaataat
    17161 gctatacagt atatggtgag accattttcg agagttataa aaaattctaa tacagcattt
    17221 tttgtaactg gatttgttat gttagttctt tcttggtttt tctggccaac tccagctgtt
    17281 gccttagttg gagctgtatt tttacctgtt gctatgagag ctgggctacc agcaattggt
    17341 gttgcagtag cattaaattt atttggtcat ggtcttgcct tgtctacaga ctttgttatt
    17401 caaggagctc caagtataac agctggtgca gcaggtgttg cagtatcata tgttataaat
    17461 gatggtatga ttttgttttg ggtgatggga atcgtaacca tatctatggc attttataca
    17521 ttaaaaagag atattaacaa aggaatgttt agagaagaac taaaaggttt tgaaagtgaa
    17581 gaagtagaag agttcaatgg aaaatctaaa atagcaacta ttttagtagc tctgggattt
    17641 cttgctgata taattgctat gtatatattt gacctaaaag gaggagatgc atcagctctt
    17701 ttgggtggaa cagcagtatt tttaataata attataaatg ttataaattt tggcaaagat
    17761 agtcttgaaa atgtatgtga gaatataatt gatggttttg tgtttgggat aaaaatattt
    17821 ggagcaatta taccaatagc agcatttttc tatatgggtg aagttgcacc attgacaggt
    17881 gtatttggaa aagtattagc accaggttca caaggtttat tatcagatat aggcatagct
    17941 ctttctcaaa caataccact taataaattt gccgtatcag gtatagaaac tgtagtggga
    18001 gcaataactg ggcttgatgg ttctggattt tctgggattt cattagttgg ttcacttgcc
    18061 tctgtatttg gaactgctat aaatgcaagt gttggtgcac tagctgcatt aggacaaatc
    18121 agtggtatat gggtaggtgg aggctgtcta gttccttggg gtcttatttc agcagcggca
    18181 atttgtggag ttagtccaat agaattggca aagcgaaact ttgtcccagt tataaccgga
    18241 ttggttgtaa caaccatagt agcaatgttt attatttaat aaatcgaata aatctttaat
    18301 tgtataaatt aaaaatagat gtgtagctat aatttattat aattgtttta ctaattctaa
    18361 gccatacatc tatttttatt gatattttta ttagtattct ttaataaaat aatatttttc
    18421 attatactat acataacatc taaatataac atctaaaata ctgttatcta tatttaatat
    18481 cttgccaaat tttttgatgt tttattgccc tgtcccacca acatatataa gctatcacaa
    18541 gtttttacct atactatcac caaacaaaaa ttctaatttt aatttattat tttataacct
    18601 atattatatt aactatatta gtttgtagac tttagatgta attataaata ttaatactcc
    18661 aagagttcaa attcatatcc tagttttctt gcatttgata taaaatcatt taaaatttct
    18721 gtactagttt tggaaactgc atgtagtaac aagatagaac catcatgtag attatccatt
    18781 attttttgcg ttgcttcttc atgtgatggt tgtttatcag tatcccagtc tctgtatgca
    18841 aaactccaaa atacagtctt gtatcctaaa tttttagtca ttgccaagct tttttctgag
    18901 tattttccca taggaggtct aaaaaattta accatatctt tcccagttac atctttatat
    18961 aacttttcaa catcatataa ttcgtcatta aagtttttta gattgcttgt ttttgttggc
    19021 atagatggat gggttttact atgatttcca actatatgtc cctcagaaac cattcttttt
    19081 actaaatctt tattttcttt tatatatgga gctgtaacaa aaaaaactgc tttaacctga
    19141 ttttgtttta gagtgtcgag tatttttgta gtgtatccat tttcataacc ttcatcaaat
    19201 gtaagatata gtgttttttg accatctttt gtaggtccat tgtatagggc atcatagtca
    19261 gaaaatttaa attcaatgtc cgtatttact tctggtgttt tatgcttatt gtttggtata
    19321 taaaaccaat caagagtagt gttatctaaa gaagatacat ctaatttatt attatcatta
    19381 ttagtagtgg cttttgatgg tttatctgtt ccgtctgaaa ctattacatt ggaagtatct
    19441 ttaccactat ctattttttc cttatcttct tgtaactgtg tttccttatt ttgattttcg
    19501 ttttgattgt tttgtgaatt tgaacatcca acaataaaaa aaatagaaat agaaagtagt
    19561 gttaaataat gcaatacttt tctaaacatt ttgaagctcc tttctaaatt aataataatt
    19621 gcatataata ataattatat gattaattac aattgcatat agtattatta taattggaaa
    19681 ttactattaa taatagttat atctttaaaa ataaggtcac aatagtatga caaagggtga
    19741 ataatcaata aatttaaaaa aaaatgaaaa aaatagttga ccatattatg agaagatgtt
    19801 attataataa atgtgctcaa caagaagtaa caaaataaca agcacaaaga agttaattga
    19861 at
//
LOCUS       DAMJYX010000065        19608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJYX010000065 DAMJYX010000000
VERSION     DAMJYX010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.065"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(71..760)
                     /locus_tag="PC479_002437"
     CDS             complement(71..760)
                     /locus_tag="PC479_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2804976.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(753..1124)
                     /locus_tag="PC479_002438"
     CDS             complement(753..1124)
                     /locus_tag="PC479_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2804977.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(1306..2652)
                     /locus_tag="PC479_002439"
     CDS             complement(1306..2652)
                     /locus_tag="PC479_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2804978.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(2834..3427)
                     /locus_tag="PC479_002440"
     CDS             complement(2834..3427)
                     /locus_tag="PC479_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2804979.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(3492..4961)
                     /locus_tag="PC479_002441"
     CDS             complement(3492..4961)
                     /locus_tag="PC479_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2804980.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(5076..6467)
                     /locus_tag="PC479_002442"
     CDS             complement(5076..6467)
                     /locus_tag="PC479_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2804981.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(6633..6881)
                     /locus_tag="PC479_002443"
     CDS             complement(6633..6881)
                     /locus_tag="PC479_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804982.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            7147..9276
                     /locus_tag="PC479_002444"
     CDS             7147..9276
                     /locus_tag="PC479_002444"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2804983.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(9503..10102)
                     /locus_tag="PC479_002445"
     CDS             complement(9503..10102)
                     /locus_tag="PC479_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2804984.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10120..13239)
                     /locus_tag="PC479_002446"
     CDS             complement(10120..13239)
                     /locus_tag="PC479_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2804985.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            13460..14284
                     /gene="pdaA"
                     /locus_tag="PC479_002447"
     CDS             13460..14284
                     /gene="pdaA"
                     /locus_tag="PC479_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2804986.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(14381..15166)
                     /locus_tag="PC479_002448"
     CDS             complement(14381..15166)
                     /locus_tag="PC479_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2804987.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            15631..15996
                     /locus_tag="PC479_002449"
     CDS             15631..15996
                     /locus_tag="PC479_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2804988.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            16074..16628
                     /locus_tag="PC479_002450"
     CDS             16074..16628
                     /locus_tag="PC479_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2804989.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            16848..17522
                     /locus_tag="PC479_002451"
     CDS             16848..17522
                     /locus_tag="PC479_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2804990.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      17877..17973
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18027..18203
                     /locus_tag="PC479_002452"
     CDS             18027..18203
                     /locus_tag="PC479_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804991.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(18374..18613)
                     /locus_tag="PC479_002453"
     CDS             complement(18374..18613)
                     /locus_tag="PC479_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804992.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(18728..19567)
                     /locus_tag="PC479_002454"
     CDS             complement(18728..19567)
                     /locus_tag="PC479_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2804993.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
BASE COUNT         6612 a         3072 c         2058 g         7866 t
ORIGIN      
        1 gtttttattt taaatttttt ttattttatt ttgttttaag tattcaagtt actcgtctta
       61 attttatttt ttatataaaa ttcattataa ttggaattag tattactgta attgtaccag
      121 agactccaat agaaacacca ctcatagctc cttctatttc tccaagttca aaagcctttg
      181 tagttcctaa agcatgtgca gatgttccaa gagcaattcc tttagcaact ggatgattta
      241 tattccccag tttgaatact gtagaagcaa ccataccacc aaaaatacct gtaaaaatta
      301 tggctacaac tgttattgat tctacaccat ttaaagaatt tgctaaagat attcccattg
      361 gtgttgttat agattttgga attagtgaat ttattatact tacatctgaa ttagctattt
      421 tgccaattat caatacagat acaaaagaaa tcactatacc acaacctatt cctacaataa
      481 tctcaaacat atgcttttta aataattcaa attgtttata aagtggaact gcaagtacta
      541 ctgtgacagg acctaaaaag aaatttagac tatcagctcc tatcttgtaa ttctcataag
      601 ggattttaga tatgcttaaa aacaatatta ttcctacgat tgcaattaat aaagggttac
      661 atataggatt acttgttttt cgttgaatgt atcttcctaa attgaaaaaa actattgtta
      721 ctattatacc aaatactggt gtctgtaata cattatacat ttttttgtcc ttcttccttc
      781 ttttttatca ttttctccac aataattcca gtagtataca ttactattag tgtacttaaa
      841 catatgacta ataaaagcac taatacattt tcccttatta ggtctaatga ctttataagt
      901 gatacacctg ctggaataaa aaatatagcc atattatcga ggaaaaatcc acttacattt
      961 tctatactac tcaagtctaa aactttaaat tgtagtagta gaaataataa tagcatacca
     1021 actatactcc caggaataag tataattcct tgtataaagg aacttatata ctctcctatt
     1081 acccagatag ataaaattat taccaattga ttgaaaactt tcattttcca cctccaaaaa
     1141 ttaaagagat tgcttcaaat catatatttg tagttttatc aacaagcaaa tatctttctt
     1201 taaaatagaa agttataatt tgacacaatc tctttttaaa tttattttta ttttaattta
     1261 ataaaataca tacttggttg tttcaaaaaa cttaactata taaacctatt tttcacttaa
     1321 aatttctttt atttttgaat tgttatacat actgtctgga acttcacttc tcaaactata
     1381 aaaagtctgt tgtgcatttt ttaaatcgcc attatcataa taaagcatac ccaactcgta
     1441 atatgaatca tcataatact ggtcattttt agtataagta tttatatatt ttttgtaata
     1501 ttttatagct tcatccttat ttcctaataa tttatttgtt attgctaatt gatatattga
     1561 ctcagaaata tattttttag ttttaccaga agaaactaca cttttaaaat tatctatggc
     1621 tttttcatat tctttgtttt tctttaaatt tagagcttga taatataaat cttcctcatc
     1681 agctacagtt atctctcttt ctggttcttt atttttaacc tcatttaatt ccttattcgt
     1741 tttttctaat tttttactcg tttctcctaa ctttttatca gtttcactta acttttcctc
     1801 tttactactt atttggttat tcaagttttc tattctcttt ttgctttgaa tcaaatatat
     1861 ccccatacct attaaaacac agccaactat aatatatggc aaaatacttt tctttctttt
     1921 tacttttttt atttcttcct ttttacttgt atcttctttt tcttcttcat ttttttcaaa
     1981 tacaggatta tctttatcta gctctttaag tttgtctaaa tatccttctc tcttttttac
     2041 attacctagt ttatcatata acaaagataa aattacatat ggctctatta gttcaggttc
     2101 ttcttcaatt atgttctcta aaatatgtat tgactcataa tttaattctt catttataaa
     2161 tcttatagaa tgattgtatc ttcctaaaaa agttttaaaa tcttcagatg aaattagatt
     2221 tacatatttt ctagatatat gattattttc ataacataaa gcagtataaa aactctcaaa
     2281 tgatttagca aaatcacatt ttaataattt taaaagacct ctaagattta gtatatcaat
     2341 gtctttagga tttatctcca gagctttatt aaccatatta atggcactag ttatataatt
     2401 tttatatgct aaatccaatg cattgttata tcttttatat gattctaatt ttaactcctt
     2461 agacgttgta tctgactttg aatttaaagc tccttgcaag tttaatagag tttcttcttt
     2521 ataatttgta gtcacataac tctgttttaa tgcttttaat gtttcatcac cgtcttcttc
     2581 ttctatatta aaaaaagtca aatcactttt attatcttct atcaaagata tatctaaaat
     2641 ttttctaggc atgctttcta tgtcttgttt tatatcttta actaaaataa tactcacctc
     2701 cactctaaaa tatatattta agataacata tttaatatgt caagtcaaaa ctatacatat
     2761 ttttccacca ttctcataat aaaaaaggta atcctcttct tttcataaaa aggttacctc
     2821 tttttcaata ctattaactt ttaatgttga atttttttac ttttctatag agtgtagcta
     2881 tacctatatt taatgcctca gctacttttt tcataccttc tgtattactt ccatatttct
     2941 ttatggcatt ttctatagct tccctctcaa ggtcatctat agtcttaact tcttgtttat
     3001 aaagttcatc tagtttaaaa gatattaaat cagacatact ccttacaaaa gcagttaact
     3061 ccttatcttt tgaaagtatc ttttcttttt gttcttcatt aaacgcagct atacccaaga
     3121 ttccaagtat atgctctccc attttaacgg gagtacatat atcagctaac tctttacaat
     3181 tttctctatt tgcacattta ttacagttct cttctatttc tttatttata acataggatt
     3241 tcccatcttt taaaacaaga gagtacactg acttctctga tattgtctct cctattttac
     3301 ctctgaatat tcctgtacct gctatcctag ttaaatcttt tgtaactaaa gtaacatcta
     3361 catccaagac attagaaata ttctcgcaaa tactttgaac gtaattcgtt atattattta
     3421 aattcaaagt aatccctcct atttagattt atatttttta ttactctaca taaatcctat
     3481 tcttgagaac tttaagcttg cggttgtggt tgtgtctcat taggcttatt atcagttggt
     3541 ggtgtttctg gtttttgttg attatttggg ttttgattgt ttgtattttg attattctta
     3601 ttttcgtctg tatttggttt ttcctctggt tttggttcca tagtcccaac taatataata
     3661 ccttccttct taggataata tgaattagct attttttcag ttttaatttt tttaccattt
     3721 gcatcattaa atgttctata tgttgataca gaatatgcat ttcttccact tttgtctact
     3781 ttttctttac ctttaggcat tgtagggtca ttaactcttt tcattgtagt ttgtgaaacg
     3841 ccatctaatt ttgttgatat tgttatattt tgtttatctt ctgcacttcc atatatattg
     3901 caaacaattt gatttcctga aacgtaattt tttatgtaaa ctggatgctt tagattattt
     3961 ttaaataaga aatctattcc actatctgca acagttgcat ctcttccttt tggaacataa
     4021 cttgatggta tagtatgatt ttttacatta acaatttcta aacctgcgta tagcacagaa
     4081 ttgtataacg tagatgaaac ttgacaaact cctccaccat aatcttcttc catctcacct
     4141 tccattatta caggtgcact tttatatcca ttttccttac ttcttttccc tgtatgttta
     4201 ttaaatgaga aagtttctcc tggcataagt aatacattac ttatattatc tgttgctaat
     4261 tttatgttta tacttctatt tatttgagaa gttttaaatg ttgttgaata cgtacctaaa
     4321 tttgtatcta tattagcaag ttgctctttt tttacttttg ctggagtagc ttttactact
     4381 aactcatttt tatagttacc ttttttagtt tgattatcaa aattctccat actcttagaa
     4441 atatccattt taagaccatc agaatctggt ataaccttga ctttttcacc acttatatca
     4501 agtgtagcat cttttacatc tacatctatt tctttagcta tcttttccat ttctgctttt
     4561 aatttatttt tatcataatt tatcgcaact actaagttgc tttttttgcc aaaatttgct
     4621 gaaatagttt ttgatagatt gccaaagaaa gcactctttc tatttaaatt atatgcgttt
     4681 tctactgttt tatccaggtc agtagataaa tttaaatctt tgctctttac tttccagctt
     4741 ttatcattgt aattaaaatc aacattctct aatttgtatt ttttagctaa ttcctgtttt
     4801 gcttgtgatt tagttaattt tccaatattt actccattaa catatgtgtt ttttgctatt
     4861 ttctcccctt taaattgcat gcacattatg cctataaaaa ttattactac taatgctaca
     4921 gctccaatag ctataactag ctttttgttc acattactca tcccatactt ctccttacaa
     4981 taaaattatt tatatatccc tattattata ttactacata atgcgcctta aaaccccaaa
     5041 aactttttaa ttatatatta atttaatatt attaattaat attcatttag tccattttct
     5101 gatatggcat atattttttc attgtaaata ttaatagcat aaacttcttt gcctctaaat
     5161 aagcctttat ttactacagc ataataataa atatttttat attttccaaa caattcttct
     5221 aaactctttt tactttttac atatctatct gctttctcta tatctaatat ctcaatcttt
     5281 ttatacttat tttctttttc atcaagagta ttagttaaca caaacttagc tttatctttt
     5341 gtaataccaa attgataact tccatcttct aatattctaa agtatccatc accataatct
     5401 actttgtaat cttttttacc atctttatat gaatataaat ctttagtttt tgcacttacc
     5461 aaaacttcat ttattaattt atcatactca tatacttcag cataatagta tttattattt
     5521 tttatcattt gtggaagtga atttatagat agatttgcct tgtctttgcc acttttttta
     5581 gctattacat ttataatgtc atcagatgtt aaagtataca atgataaatt ctttttatat
     5641 tttttttcta aactctgtac cttttcatca tctgaataat cgtcttttaa atactcaata
     5701 tattgtaatg ctctttcata atctccagaa gtatttgcct cttcagctct acttatatag
     5761 taatcataca tttcttcaat acattgttct ttcccatttt gagccatact gtaatacttt
     5821 tcatcttctt ttaatacttt attatacttt tcaatagctt cataatactt atatgctttt
     5881 tggtctttta ctgctgagtc ataaacatct ctagactctt tgattgtttc tatattctgt
     5941 ttgtatacat ctaggttact acccatacca tttaatattg aaacagtact tatgtagtta
     6001 atggcaatat cataagtagt attttcttct ttatacattt ctctaactct atcagcttga
     6061 gctaataagt catccacatc tatacttatt ttgtttaatt ctttcacggt atttattaat
     6121 ccaataaaac tttcttgact tatgtctcca tctaaatatt tatcaccact atttaatagc
     6181 aatttattta tttttttaga aacagactta ttaaaccttt ccatttttga atcagaaaac
     6241 tttttttcta tacttgtgta gtaatcaaca gctatagaat actgtctttt ctctataatc
     6301 tttaacaact cattagcttc atttcctaca ctaaactcgt tactacttaa gaaaaatact
     6361 attatcaaag caattacacc taatgttgat aatgaaatcg caactataga tttccttttt
     6421 tcatcaataa aatctacagc cttctcaaat aaatctctaa aattcattat agttccctct
     6481 ccatttaata atctttatat tttatggtaa actaaatttc ttattggtta ttatatcata
     6541 attcttgaaa aaaaataaaa acacaagtta aatcgtgttt ttattttacc tatcaatcat
     6601 tattttatac ataaatattt cctttacata aattatgata tctttctttt tatatcccta
     6661 gaatgactct tttgtttttt attattactg acagttttcg ttcttgtctt gtgtatagct
     6721 ttgctatcaa atatttttct agcattgcat aaaacaattc caattgttaa acaaactgta
     6781 agcacacttc ccatcaaaaa gtaataatcc aatcctatat gtactcccat aactgaattt
     6841 attaacacta atgcaaaagt cattgcaaaa catatcatca tggagataac taaaattctt
     6901 agaatttttt tcaatccact cacctcgtat aatcactaaa attgacacta ccccttatcc
     6961 attactatac caaattagta aaaaaatagc aattactatt gtcatttata ttaggaaaat
     7021 attaaatctt tttaataaat atcttttttt gacataagga ttacttaact tttaataaaa
     7081 tagtaacact aatatgtatt aattaataga ataaaataaa tgataattta ttgaggggtg
     7141 atttacatgc caaatttgcc aagtttaggg tcaaaggctc ctgattttaa agccaataca
     7201 acaaatggtc ctattagact ctctgactat aagggtaatt ggattgtttt attttcacat
     7261 cctggtgatt ttacaccagt ttgtactaca gaatttttat gttttgctaa atattatgac
     7321 gaatttaaaa aaagaaatac agaactaatt ggtctaagtg ttgatagtaa cagttcacat
     7381 ttagcttgga tgtataatat ttctttactt acaggtgtag aaattccatt tcctattata
     7441 gaagatagag atatgagaat tgccaagtta tacggcatga tatcaaaacc aatgagtgat
     7501 acatcaactg ttcgctctgt atttattata gataataatc aaattctaag aacgattctt
     7561 tattatccac taactacagg aagaaatatt ccagaaatac ttagaatagt agatgcactt
     7621 cagactagtg atagagataa tatagttact cctgcaaact ggtttcctgg aatgccagtg
     7681 attttacctt atcctaaaaa ctataaggaa ttaaaaaata gagttaacag ttgtaataag
     7741 aaatattcat gtatggactg gtacttatgt tttgtaccag ataattataa tgatgaagaa
     7801 gtgagcaaga aaattgataa tacttgtagc tggaaaaaag aacatactaa aaacattgaa
     7861 aatgaatgta actgtgaaca tgaacatcat gactacctga acaaagctct tgactgtaaa
     7921 caagaacaca agactgatat taaagatgat tgcaatcatg agaaaaaaca tactaaaaat
     7981 actaacaaag ttcacaactc caaacaagat aagtttaaag ataagtcttg tgatgaaatg
     8041 aattttaact atgataaaga tgaatcttgt gacaaaataa attctagcta taacaaagaa
     8101 gatagtagtt atgaagattt ctataaacat aattataaaa actacgatta tactagcgaa
     8161 aaaaatacta aaaaaatagc tatgaaaact ttaaaagatt ctaaaaaatt agttagacca
     8221 caaataacag acccatacaa tccaatagtt gaaaatgcaa actgtccaga tataaatcca
     8281 attgtagcag aatatgttct tggaaatcca actaatgtag atgctcaact attagatgca
     8341 gttatatttg cttttgctga gatagaccag tctggaaatt tgtttattcc ttatcctaga
     8401 tttttaaatc aattacttgc tcttaaaggt gaaaaaccta gcttaaaagt aattgtagct
     8461 attggaggtt ggggagctga aggattctct gatgcagcat taacacctac atctagatat
     8521 aattttgcaa gacaggtcaa tcagatgata aatgaatatg ctttagatgg aatagatata
     8581 gactgggaat atcctggaag tagtgcatct ggaataacat caagacctca agatagagaa
     8641 aactttacac tcttactaac tgccataaga gatgttatag gagatgataa atggcttagt
     8701 gtagctggaa caggagatag aggatatata aattcaagtg ctgaaataga taaaatagct
     8761 cctataatag attattttaa tcttatgagt tatgatttta cagcaggtga aacaggccca
     8821 aatggtagaa aacatcaagc aaatcttttt gattcagact tatctttgcc aggatatagt
     8881 gttgatgcaa tggtaagaaa tcttgagaat gctggaatgc cttctgaaaa aatccttctt
     8941 ggtataccat tttatggaag attaggtgct actataacaa gaacttatga tgagcttaga
     9001 agggattata taaataaaaa tggatatgaa tatagatttg ataatactgc tcaagttccg
     9061 tatttagtta aggatggaga ttttgcaatg tcatatgatg atgctttatc aatattctta
     9121 aaaactcaat atgttcttag aaattgtcta ggtggtgtat tctcatggac atcaacttat
     9181 gaccaagcaa atatactggc tagaaccatg tctattggta taaatgaccc tgaagtatta
     9241 aaagaagaac ttgaaggtat ttatgggcaa ttctaataga atgtagtaaa attttatagt
     9301 tatataaaaa gcagatttta ttatattaaa taagttgtat ctaattaata ctattattaa
     9361 ttgtttataa attagatact tattttttca taatacaatc tgctttttta atcaaaattt
     9421 atttaattac aagttgttta attataaagt aggaatttag atatactctt ataccatgta
     9481 attatgtata aatatttttt tattatacac caatagcttt tttagctaat ttatcagctt
     9541 cctcattata cttatttcca gaatgagctt taacctttgt aaattttaca ttaagcttat
     9601 tcttaatact gtcatagaaa tttttatatg ctatagttcc ttctttattt gttttccaaa
     9661 ctccagtaca ccacttttca atcccttcat agtcaaagta taaaatcaag ttttgaacat
     9721 tattgtcaat acagtactgc attgcaattt tagaagcctc tatctctcca gctacatttc
     9781 tcatacttac taaagttttc tcttttcctt tttcactata agtttttata acttcattat
     9841 tttttaaaat cacaacaccc gaaccataca tttttactga atgttcataa cttccatcta
     9901 catatgcttc tataaactcc ttatcttctt gaaaactcaa tttttctgac ccatcaatat
     9961 attctttagc ttcttgaaaa gtagaaaaac tcttatattc agctcctgag aatccattta
    10021 cttgtttttt gcactcatcc catgtattat atacacctat atttttacct tttttcacag
    10081 cataaaactt ttgttttgcc aaatatatca ctcccactct taatttttat cttcttctct
    10141 cataaatata tctaaattta taagatagtt tctatcaaaa tattttactt tcatatcttt
    10201 ttttataata tcaatatggt acacttctcc ttttacagct ctttgtctta tattttcatt
    10261 tatatctttt tttataatat ccttatcaac atcattgtta tccattttag catcaacatt
    10321 caacgctttt atagtatcaa atataacaga ttttagtatt aaacttctag atttatttaa
    10381 atgtcgcctt atataagtat aaaactcatt gctattaacc tttgaaattt tacaatcgtg
    10441 taaatctttc tctaatctct ttaacacact tatattagtt cctacatcaa aaatcacaaa
    10501 acatccatag tcatatttgc ttcttaaaat tctccccata gcttgcttta cctttatggt
    10561 catttgagga taatttatag tatagtagtc tataccatac ttcttcatta tagtaaaata
    10621 caaagggtct ttaggattta gattcggaag cttgtctaaa gttacacaaa ccaaaccatc
    10681 ccctggaatg tctacgcctt caaaacatcc ttttgagcca agtactacac attttctatt
    10741 taaatcgtta aggtgtctaa tgcccttttt atcagcataa atttctaagt taaaactatg
    10801 taagtatttt ttcaatattt cataagtttt ttcttgtcta tctttactat taaaaagagc
    10861 taatgtatga ccttctgtaa tctttgatat attagatata attttactca tctcatttgg
    10921 aaattcagaa tttctgtact cacaaatatc agaaaatcca actacagaga ctctattttt
    10981 ataatcaaaa attggttcta taatcttttc cacattctta actctgtcta ttcctaatgt
    11041 atttttaaaa taagacatac tttcagataa actcaaagtt gctgataaaa atattccttt
    11101 ttctaattga gataaaatat tttcttcaaa taaatctgct atttttaatg gtactactcg
    11161 aaattcaaag tcattataat tcttatcaat ttcaactatc cttgcataat catccttttc
    11221 atcatactct aaaaagattt caaatataat ctttatatcc tctaaatctc ttgtctttgc
    11281 ctttccaaat ttatatatat ctgcttcttt atctatacta tcatcatcaa tattcctata
    11341 aattattatt attattgaaa ctaaattttt tattattttc tcacaactca actttatctt
    11401 ttcactatag gctctataac ttatttcagt atctatacca tcttttttta gttttcccac
    11461 aatttcatct atttgcaaat tcagttccca tcttaaatta taattactta cattattata
    11521 ttcattacaa ttaccaaact ttaatattga atctatctct tcaataatga aatttatact
    11581 tctagcaatt ttttgtttat cttctcttcc aatttttaat acattataaa atttatcaaa
    11641 agcctttatt tttctcatat ttctactata ttttttatat ataaagctac tattttgaat
    11701 aaactcgtaa ggataaattt cttgtaataa gtaccttaaa gactttgaat ttataatact
    11761 tgaaaagaaa tcatatcctt tttcagtcaa attatgtgct tcatctacta ttatattttc
    11821 taaaggtttt tcatccttat acggccattt agctaatagt gaatgattta ctactgttat
    11881 atgttcttct tttaattctt ctattctgtt tttatataaa caatcttttt tgcatttttt
    11941 aggtctgcac atatttgggt cacaacttac atttcttaag tgagtactta tttccttgaa
    12001 gttgtcaaac actaaataat ttatttcttc tatatctcca tacttgccac cttcaactaa
    12061 tctcttcaaa aatattaaag ataaaatttc tcctttagta gggttttgag actcataatc
    12121 atctatatat gcttccagtc tatctataca aatatagtta ttttttccct ttatatatcc
    12181 gtaacttact tttccattta gacccaaaga atctaacaca tttggaatat ctttatttat
    12241 taattgtatc tgcaattcct ttgtgtcagt agaaattaat aatctttttt tattaattct
    12301 agcctctaat attgcaggca ataaatatcc tacactttta cctataccag ttggtgcctc
    12361 tatacatgca attttttctt catcttcgct atttctaaac aactctctta ttgttttagt
    12421 aagctcatat tgaccaggtc tatattcata tgtaaatcct tctttattct cccaaatagt
    12481 tttgtatttt aataattctt cataattttt ttcttcttca tttaaaattt ttaaggtttc
    12541 tctttccttc ttcttttctt cttttgagtc aaatatatta tattcttttt ttatatttat
    12601 attatttgat aaatcataat ttgcctcttc taaaaatttg ctccattccc atgttggaag
    12661 attaaatttt ttaagatatg aatttatttt aaaactcaat ggctccaaag tagttttttc
    12721 atagttatta aattttacta aaaggcaatt tacaatattt attatatcaa tagcatctga
    12781 taaagctcta ttttccactt tagaattaca atttgttaaa gtatttttta agtattctaa
    12841 gctataatct ttatgatatg gttctaaaat aactgctaat tcaattaaat caataaattt
    12901 attttttaac tttggcatat gatactcaaa aaactttttt ttcagactta aatcatgaca
    12961 tataatgaaa ctatcttcta caaaatttac aagtctatct tccaccagtt ctattttatt
    13021 agctatttct aaatcatttt tatctaaatt tttacatact gagaatactt ctataggtac
    13081 ctcaaatttg ttttttatta atgattcata ggtatatatt ttccagtctt taaccttgac
    13141 agctccaact tctaaaattt ctgaatttaa acaatctaat ccactaactt ctatatctaa
    13201 aaaaacaaca tcattcaata cacttattat attatccatt tttatatccc ttcaagttaa
    13261 accagcctaa cttatttaca atcatttctc tttatatttt atcatttata tacactttat
    13321 ttaagataat taatatcaac tttatatctt acagtaaact tctttcattt attatttgta
    13381 tatgtgaata tttttaacta taaatattca aactatatta taagaaatat actttaaatt
    13441 ttaaataaga ggtgaaattg tgaaaaaaga tagcctaaaa aaatacatta tgataggagc
    13501 atttgctcta acactttttg gaattgctag tataaatttt aaatcattag ataaaacaaa
    13561 aactcaaatt tctagtccaa cactcgacac acatgaatat gattggtatt ttaaccctag
    13621 ggaagatggt aaacagccat cacctataaa agaagctgat ttttttaaaa aatacggagc
    13681 ttattatgta ggaaatccca atgaaaaagt tatatatcta tcatttgatg ctggatatga
    13741 aagtggaaac actccaaaat tattagatac gcttaaaaaa cacaatgcta aagctcaatt
    13801 tttcgtagtt gaaagttata taaaaagcaa tccagagtta ataaagcgta tggaaaaaga
    13861 aggacattta gtttgtaatc attcaaaaag ccatccatca atggcaggta ttacagattt
    13921 tgaaaaattc aaagaagaaa ttacaagtgt agaaaaagca tacaaagatg ttactggaaa
    13981 agaaatgcct aagtatttta gacctccaat gggtaaattt agcgaacaat ctttaaaata
    14041 tacacaagat ttaggatata aatcaatatt ttggagtttt gcttatgtag attggtatga
    14101 aaagaaacaa cctactcatg aatttgccaa aaacaaaatt tattcaagaa ctcatcctgg
    14161 tgcaatagtt ttactgcatc caaattcatc aacaaatact gagatattag atgaggttct
    14221 tacacattgg gaaaaagaag gttataaact taaaacactt gattatttaa ataataaaaa
    14281 atagtatcat aaatttaaaa aaactctatc atgaattata gtaaaaatat agttatttat
    14341 aaatacttta attctcgata gagtttttta ttttttatag ctatttaaaa agaattgtcc
    14401 attcatttat ctctttgtat cctatattat gatatatttt acctgctaca tcattattat
    14461 aaaaaagaca tggaactttt ccttctaaca acaattcttt actcaaattg taaaccatat
    14521 aacttgcaag tccttttccc ctatatcttt tatctgtact tacagagacc accattgcca
    14581 aaaaactagt ttccatacca gttgacacag tgctaatcat agtatcatca tttctaataa
    14641 aatatgctct tacatttccc tcttttaaat gaacttttct ttcttctatg taatttttac
    14701 ttactttata gtctgaccta tttaaaagtt ttcctatttc atcaatatct ttttcctgag
    14761 cattttctat tttatactcc ttcatgttgg aaaaatctat ttcttttatt tcttttaata
    14821 cacaaaattt attatctagt ttttcataaa aatctttgta tttacttatt agtctatcta
    14881 ttacacattt ttttccactt atgctttcta catctaaatc ttttatatga ttcttcattt
    14941 ctcctatatt aaaattattt ttaaaactat aaattataag tgttgactta ttttttaata
    15001 ttatagaggt tatatttcca ccttctacct gagaccatat atcttgatat tgagagctaa
    15061 atccataatt ctcaacgtat ccaattatga ataaatttaa actttcttca tgtgatacat
    15121 attgccaaaa ttctttttct aaagtattat ctatttttac aatcaagcag atatcctcct
    15181 tctatgttaa taatgatata ccttataatt tatagtatca tttaaaatca ttatattcta
    15241 ccaaatattc taaattttta aatatctatg caatatgttc gtatttattt ttatattctg
    15301 ttactgcttc actccaagct tcttctactg aaatacctct cttatttgca attttttgtg
    15361 ctaattctat tattttcata gcttttcttg atatattcat atatattcct cctctaaatt
    15421 atcataagtt attttaaagt taaatttcta tagtcatgtt atatatttat ttacttatat
    15481 cttactaatt atttatgtac taaatatgaa ctacaataga agaaatttag aacttaataa
    15541 aacataaaat ttatatacag ttcatatata tttgtcatat cacaagacaa gtgatataat
    15601 ttattatcat tttactgggg gtgatatgct atgaaaataa gagctgaata tacatgtcct
    15661 cttgaaattg tacatgacat aatcaaagga aaatggaaaa ctatccttat atttgaatta
    15721 agacaatcta ataaaacatt ttctgaattg caacataata tttatggaat aaatcaaaaa
    15781 atgttgattc aacaattaaa agagttacgt gattttggga ttattgataa aataagttct
    15841 gctggatatc cattacatgt tgaatatttt cttactaatc gtggtaaaaa aatgttaaaa
    15901 gcagtggaaa taatgcaaga aataggaatc gaatatatgc ttgaacatgg tcaacaagaa
    15961 attctagata ataaaggaat ttgctacaat aagtaatcct atacatacca aaaagtaagt
    16021 aatttacatt ttttacaata gtattataat aaaaataaat aggaggtata atcatgtcta
    16081 tagctttaat attgattgat attcaaaatg attattttaa aaatggaaaa tgtgaattat
    16141 ttcaatcaga agaaactgct gaaaatgcta aaaaaatact actgtttttt agaaaaaaca
    16201 atttaccagt tattcatata cagcatatat caacagataa aacagcttct ttttttcttc
    16261 ctaatactca tggttcagaa ataaataaaa ttgtttttcc actagataat gaagaaatta
    16321 taattaaaca cacaccagat agtttctttg aaacagattt gcaaagtatc cttgagaaaa
    16381 aaaatattac taatctagta atctgtggaa tgatgagcca tatgtgtatt gatacaagtg
    16441 taagaacagc aaagaaactt cgatacgaca taactctaat aagcgatgca tgtacaacga
    16501 agaatttatc ttggaataat atagaaatta atgcaaatac tgtacatcaa gtattcatgg
    16561 catcactaca gaacagtttt gcagatgtaa aaaacacaga taaattttta tctaactatt
    16621 acaaataaaa acatacacta tgttaaaaga gcaagtttga tttatttaag cttgctcttt
    16681 aatcatttaa aattagatgc tttctatcat ttttatatac ataatatacg tttatacatt
    16741 ttatattcat atataactat tatataaatt ttaaacttta ctatattcac ataaaaaatg
    16801 tagtattata gtatatataa tattatatag aagggggtgc tagataaatg aaccatcata
    16861 tattgattca atttgaatat atagcaagaa tcattactgc tggtttctgt ggggctttaa
    16921 ttggatatga gagaaagaat agactaaaag aagctggaat acgcactcat tttatagtgg
    16981 cattagcttc agcacttata atcattgttt ccaagtatgg atttaatgat atagtgggta
    17041 ctccaggtat tggtctagac ccagcccgcg ttgcagcaca agttgtcagt ggtataggtt
    17101 ttttaggtgc tggattaata tttattagaa atcaatcagt aagtggtctt actacagctg
    17161 ctggtatatg ggcaacagcc ggtacaggtc ttgccattgg ctctggatta tacttagttg
    17221 gtattgtttc aagtatcatt attgttattg tacagataac acttcataga gataggaaat
    17281 ggatgaaact ccctacagca gagcaagttt ctcttaaaat ttctaacagc actgattcca
    17341 tagaatatat tgaaggtaaa tttaaaaaat ctaatattga aattataaac atgaatatta
    17401 aacgccttga tggggattgg ttagaagtag ttatatatac taagttacct aaaggatata
    17461 aaaaaacaaa tttattaaaa ttatttagcg ataatcctct tatagaaact ttagaaatat
    17521 aatataaaaa ttgaagctta aaaataataa tatatttaat caaggtcaat atataaataa
    17581 ttaagggatg agtctatata aaattcatcc cttgtttttt gccattcata tatatttttt
    17641 caagatattt ttgtttatct attatctagc caagtaaccc ttgtgaacta aactttttaa
    17701 tacaaaaact tcaatacaaa actaaatata atttttatta aacattctat acatatagtt
    17761 tgtttcttga gtgaaaaata gatttcatat tgttaagata ttgttaaata tttcaatttt
    17821 ctgacaagta tgttgaacaa taacttatcc tagtgtatta ttttagttgt agaataaaac
    17881 taaatcggca aaactagaga aatttagtga cgcaaagcta tagggactaa cgcttataaa
    17941 ataaactatg agttatgtca gccagttgcc aaaaagatat tgttcttttt gttttatgaa
    18001 agttttttag ggggatagta atatatatgt ttagagatga aatggataaa tgtacacaca
    18061 tgttaactgc ttatattagt agtttatatg attattgtga ttttatagat acacagctag
    18121 atgattttat actagagtac ggagaaaatg tagtagaatc ttgtttacat caagtgatgg
    18181 tattggtaag taagtataat taaatagaaa ttgttttcta gagaaccctt tttagtttta
    18241 tctacattta aaaatgttat tatattaaaa aactaatctt ccatatatgc aaagttttct
    18301 tccatataac aatttcttcc catttatact ttttcacttg tatctttaat aaaattcaac
    18361 ttcaaaaatt attctatttc ttatctttac accaattcct atcagtatta tcctttatat
    18421 aggttctaac tatttcgcca caatctaaac ataaatctga atatatagta gattgtactg
    18481 gaaaagtttt atttataact ttatctaatt ttccatctcc tctatatttt aatccaatag
    18541 tttcagcata tatgccacca gttgttaaaa ttcccttttc aatatttaca cttccacaat
    18601 tagaacattt cacatttata accccctaaa caattatttt ttcatgctat cttataattt
    18661 aaaatataat ttgctaccga ttttataaca cagtggcaaa tcggtgccaa atcaacattt
    18721 tttcattcta tttaccatct aatccattga aatatagata tttagttata ggaacttgaa
    18781 tttctgttac aaactcctct acaattccag tctcatgtat atctatctta tagatttcta
    18841 tagggtcagc aattatctta taattatttt cttctataaa tttaaacacc ttctccatat
    18901 acatcttatt attagaatta ctcccagtat aatttaaagt tacataatat ccttcatcaa
    18961 aaactatatt atatactttc tcattacttt ccagaaaaca aaatacagat ttaaactcat
    19021 taaatatgcc tttattaatt gcttcagtag aaaaggcaga accaatattg ttgtttccaa
    19081 gtatattaaa tctatcttca tattctttct gaagtttttg aattaaaaaa tcgaagtctt
    19141 catccctctt tatatcagca tttaattcta aagcttttct tttttctata tatacaacct
    19201 ctatctcatc tatttttgta ttttgaatta cactttcaat tgaactcaat ctttttataa
    19261 tattttcctt atggcttata aattcagcta tttttttatc aattaaatcg agttcttcat
    19321 ttaaaatatt tttcgtagac tctatacttc tgtcatctag gtattctttt attttcttca
    19381 ttggaaagtt caagcttcta aattctttta ttaaatttaa tttccatata tctgatatat
    19441 tatacattct atatccattt atatctctaa ctggtcttag aatgcctatt tcttcataat
    19501 acattaatga atctctacct attccatata ttttagatat ctctcctatt ttatagtaat
    19561 ctttcattta tataataaac tcctccgaaa tcttattttt attttaaa
//
LOCUS       DAMJYX010000066        18834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJYX010000066 DAMJYX010000000
VERSION     DAMJYX010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.066"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            221..775
                     /gene="tcdR"
                     /locus_tag="PC479_002455"
     CDS             221..775
                     /gene="tcdR"
                     /locus_tag="PC479_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2804994.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1093..8193
                     /gene="tcdB"
                     /locus_tag="PC479_002456"
                     /gene_synonym="toxB"
     CDS             1093..8193
                     /gene="tcdB"
                     /locus_tag="PC479_002456"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2804995.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8315..8815
                     /gene="tcdE"
                     /locus_tag="PC479_002457"
     CDS             8315..8815
                     /gene="tcdE"
                     /locus_tag="PC479_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2804996.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8894..9055
                     /locus_tag="PC479_002458"
     CDS             8894..9055
                     /locus_tag="PC479_002458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804997.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9120..9326
                     /locus_tag="PC479_002459"
     CDS             9120..9326
                     /locus_tag="PC479_002459"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2804998.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            9543..17675
                     /gene="tcdA"
                     /locus_tag="PC479_002460"
                     /gene_synonym="toxA"
     CDS             9543..17675
                     /gene="tcdA"
                     /locus_tag="PC479_002460"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2804999.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(18010..18708)
                     /gene="tcdC"
                     /locus_tag="PC479_002461"
     CDS             complement(18010..18708)
                     /gene="tcdC"
                     /locus_tag="PC479_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2805000.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7444 a         2077 c         2903 g         6410 t
ORIGIN      
        1 tttttacaat actttattaa tataaagttt attgctaaaa tactttattt attagaaaaa
       61 gattactaat taattattaa aattaatgta ttcataatgt atattttcat ataaaattta
      121 atttatttgc cgattatata attataatga ctgatttaat tccaatgttg tcaaaatttt
      181 caaataaatc atcattataa atataagaga ggatgatttt atgcaaaagt ctttttatga
      241 attaattgtt ttagcaagaa ataactcagt agatgatttg caagaaattt tatttatgtt
      301 taagccatta gtaaaaaaac ttagtagagt tttacattat gaagagggag aaacagattt
      361 aataatattt tttattgaat taataaaaaa tattaaatta agtagctttt cagaaaaaag
      421 cgatgctatt atagtcaaat atattcataa atcattactg aataagactt ttgagttgtc
      481 tagaagatat tctaaaatga agtttaattt tgtagaattt gatgaaaata tcttaaatat
      541 gaaaaataat tatcaaagta agtctatttt tgaggaagat atttgttttt tcggatatat
      601 tttgaaagaa ttatctggta ttcaaagaaa agttattttt tataaatatt taaaaggata
      661 ttctgataga gaaatatcag tgaaattaaa aatatctaga caagctgtta ataaggctaa
      721 aaatagagca tttaaaaaaa taaaaaaaga ctatgaaaat tattttaact tgtaattaat
      781 gagtttaaag aaatatttac aatagaaatc aaattttata attaacttta ttgtaaaatt
      841 aataacttaa tctaagaata tcttaatttt tatattttat atagaacaat gtttacatat
      901 ttatttcaga caacgtcttt attcaatcga agagcaaatt aatcaactga gtgtcttaaa
      961 tttaaaatgt taggaagtga atgtatatga aaacctaagt agatattagt atattttata
     1021 aatagaaagg aggatatata aaagagtttt agcatttaga tgtaaaaata ttatagtaaa
     1081 ggagaaaatt ttatgagttt agttaataga aaacagttag aaaaaatggc aaatgtaaga
     1141 tttcgtactc aagaagatga atatgttgca atattggatg ctttagaaga atatcataat
     1201 atgtcagaga atactgtagt cgaaaaatat ttaaaattaa aagatataaa tagtttaaca
     1261 gatatttata tagatacata taaaaaatct ggtagaaata aagccttaaa aaaatttaag
     1321 gaatatctag ttacagaagt attagagcta aagaataata atttaactcc agttgagaaa
     1381 aatttacatt ttgtttggat tggaggtcaa ataaatgaca ctgctattaa ttatataaat
     1441 caatggaaag atgtaaatag tgattataat gttaatgttt tttatgatag taatgcattt
     1501 ttgataaaca cattgaaaaa aactgtagta gaatcagcaa taaatgatac acttgaatca
     1561 tttagagaaa acttaaatga ccctagattt gactataata aattcttcag aaaacgtatg
     1621 gaaataattt atgataaaca gaaaaatttc ataaactact ataaagctca aagagaagaa
     1681 aatcctgaac ttataattga tgatattgta aagacatatc tttcaaatga gtattcaaag
     1741 gagatagatg aacttaatac ctatattgaa gaatccttaa ataaaattac acagaatagt
     1801 ggaaatgatg ttagaaactt tgaagaattt aaaaatggag agtcattcaa cttatatgaa
     1861 caagagttgg tagaaaggtg gaatttagct gctgcttctg acatattaag aatatctgca
     1921 ttaaaagaaa ttggtggtat gtatttagat gttgatatgt taccaggaat acaaccagac
     1981 ttatttgagt ctatagagaa acctagttca gtaacagtgg atttttggga aatgacaaag
     2041 ttagaagcta taatgaaata caaagaatat ataccagaat atacctcaga acattttgac
     2101 atgttagacg aagaagttca aagtagtttt gaatctgttc tagcttctaa gtcagataaa
     2161 tcagaaatat tctcatcact tggtgatatg gaggcatcac cactagaagt taaaattgca
     2221 tttaatagta agggtattat aaatcaaggg ctaatttctg tgaaagactc atattgtagc
     2281 aatttaatag taaaacaaat cgagaataga tataaaatat tgaataatag tttaaatcca
     2341 gctattagcg aggataatga ttttaatact acaacgaata cctttattga tagtataatg
     2401 gctgaagcta atgcagataa tggtagattt atgatggaac taggaaagta tttaagagtt
     2461 ggtttcttcc cagatgttaa aactactatt aacttaagtg gccctgaagc atatgcggca
     2521 gcttatcaag atttattaat gtttaaagaa ggcagtatga atatccattt gatagaagct
     2581 gatttaagaa actttgaaat ctctaaaact aatatttctc aatcaactga acaagaaatg
     2641 gctagcttat ggtcatttga cgatgcaaga gctaaagctc aatttgaaga atataaaagg
     2701 aattattttg aaggttctct tggtgaagat gataatcttg atttttctca aaatatagta
     2761 gttgacaagg agtatctttt agaaaaaata tcttcattag caagaagttc agagagagga
     2821 tatatacact atattgttca gttacaagga gataaaatta gttatgaagc agcatgtaac
     2881 ttatttgcaa agactcctta tgatagtgta ctatttcaga aaaatataga agattcagaa
     2941 attgcatatt attataatcc tggagatggt gaaatacaag aaatagacaa gtataaaatt
     3001 ccaagtataa tttctgatag acctaagatt aaattaacat ttattggtca tggtaaagat
     3061 gaatttaata ctgatatatt tgcaggtttt gatgtagatt cattatccac agaaatagaa
     3121 gcagcaatag atttagctaa agaggatatt tctcctaagt caatagaaat aaatttatta
     3181 ggatgtaata tgtttagcta ctctatcaac gtagaggaga cttatcctgg aaaattatta
     3241 cttaaagtta aagataaaat atcagaatta atgccatcta taagtcaaga ctctattata
     3301 gtaagtgcaa atcaatatga agttagaata aatagtgaag gaagaagaga attattggat
     3361 cattctggtg aatggataaa taaagaagaa agtattataa aggatatttc atcaaaagaa
     3421 tatatatcat ttaatcctaa agaaaataaa attacagtaa aatctaaaaa tttacctgag
     3481 ctatctacat tattacaaga aattagaaat aattctaatt caagtgatat tgaactagaa
     3541 gaaaaagtaa tgttaacaga atgtgagata aatgttattt caaatataga tacgcaaatt
     3601 gttgaggaaa ggattgaaga agctaagaat ttaacttctg actctattaa ttatataaaa
     3661 gatgaattta aactaataga atctatttct gatgcactat gtgacttaaa acaacagaat
     3721 gaattagaag attctcattt tatatctttt gaggacatat cagagactga tgagggattt
     3781 agtataagat ttattaataa agaaactgga gaatctatat ttgtagaaac tgaaaaaaca
     3841 atattctctg aatatgctaa tcatataact gaagagattt ctaagataaa aggtactata
     3901 tttgatactg taaatggtaa gttagtaaaa aaagtaaatt tagatactac acacgaagta
     3961 aatactttaa atgctgcatt ttttatacaa tcattaatag aatataatag ttctaaagaa
     4021 tctcttagta atttaagtgt agcaatgaaa gtccaagttt acgctcaatt atttagtact
     4081 ggtttaaata ctattacaga tgcagccaaa gttgttgaat tagtatcaac tgcattagat
     4141 gaaactatag acttacttcc tacattatct gaaggattac ctataattgc aactattata
     4201 gatggtgtaa gtttaggtgc agcaatcaaa gagctaagtg aaacgagtga cccattatta
     4261 agacaagaaa tagaagctaa gataggtata atggcagtaa atttaacaac agctacaact
     4321 gcaatcatta cttcatcttt gggggtagct agtggattta gtatactttt agttccttta
     4381 gcaggaattt cagcaggtat accaagctta gtaaacaatg aacttgtact tcgagataag
     4441 gcaacaaagg ttgtagatta ttttaaacat gtttcattag ttgaaactga aggagtattt
     4501 actttattag atgataaaat aatgatgcca caagatgatt tagtgatatc agaaatagat
     4561 tttaataata attcaatagt tttaggtaaa tgtgaaatct ggagaatgga aggtggttca
     4621 ggtcatactg taactgatga tatagatcac ttcttttcag caccatcaat aacatataga
     4681 gagccacact tatctatata tgacgtattg gaagtacaaa aagaagaact tgatttgtca
     4741 aaagatttaa tggtattacc taatgctcca aatagagtat ttgcttggga aacaggatgg
     4801 acaccaggtt taagaagctt agaaaatgat ggcacaaaac tgttagaccg tataagagat
     4861 aactatgaag gtgagtttta ttggagatat tttgctttta tagctgatgc tttaataaca
     4921 acattaaaac caagatatga agatactaat ataagaataa atttagatag taatactaga
     4981 agttttatag ttccaataat aactacagaa tatataagag aaaaattatc atattctttc
     5041 tatggttcag gaggaactta tgcattgtct ctttctcaat ataatatggg tataaatata
     5101 gaattaagtg aaagtgatgt ttggattata gatgttgata atgttgtgag agatgtaact
     5161 atagaatctg ataaaattaa aaaaggtgat ttaatagaag gtattttatc tacactaagt
     5221 attgaagaga ataaaattat cttaaatagc catgagatta atttttctgg tgaggtaaat
     5281 ggaagtaatg gatttgtttc tttaacattt tcaattttag aaggaataaa tgcaattata
     5341 gaagttgatt tattatctaa atcatataaa ttacttattt ctggcgaatt aaaaatattg
     5401 atgttaaatt caaatcatat tcaacagaaa atagattata taggattcaa tagcgaatta
     5461 cagaaaaata taccatatag ctttgtagat agtgaaggaa aagagaatgg ttttattaat
     5521 ggttcaacaa aagaaggttt atttgtatct gaattacctg atgtagttct tataagtaag
     5581 gtttatatgg atgatagtaa gccttcattt ggatattata gtaataattt gaaagatgtc
     5641 aaagttataa ctaaagataa tgttaatata ttaacaggtt attatcttaa ggatgatata
     5701 aaaatctctc tttctttgac tctacaagat gaaaaaacta taaagttaaa tagtgtgcat
     5761 ttagatgaaa gtggagtagc tgagattttg aagttcatga atagaaaagg taatacaaat
     5821 acttcagatt ctttaatgag ctttttagaa agtatgaata taaaaagtat tttcgttaat
     5881 ttcttacaat ctaatattaa gtttatatta gatgctaatt ttataataag tggtactact
     5941 tctattggcc aatttgagtt tatttgtgat gaaaatgata atatacaacc atatttcatt
     6001 aagtttaata cactagaaac taattatact ttatatgtag gaaatagaca aaatatgata
     6061 gtggaaccaa attatgattt agatgattct ggagatatat cttcaactgt tatcaatttc
     6121 tctcaaaagt atctttatgg aatagacagt tgtgttaata aagttgtaat ttcaccaaat
     6181 atttatacag atgaaataaa tataacgcct gtatatgaaa caaataatac ttatccagaa
     6241 gttattgtat tagatgcaaa ttatataaat gaaaaaataa atgttaatat caatgatcta
     6301 tctatacgat atgtatggag taatgatggt aatgatttta ttcttatgtc aactagtgaa
     6361 gaaaataagg tgtcacaagt taaaataaga ttcgttaatg tttttaaaga taagactttg
     6421 gcaaataagc tatcttttaa ctttagtgat aaacaagatg tacctgtaag tgaaataatc
     6481 ttatcattta caccttcata ttatgaggat ggattgattg gctatgattt gggtctagtt
     6541 tctttatata atgagaaatt ttatattaat aactttggaa tgatggtatc tggattaata
     6601 tatattaatg attcattata ttattttaaa ccaccagtaa ataatttgat aactggattt
     6661 gtgactgtag gcgatgataa atactacttt aatccaatta atggtggagc tgcttcaatt
     6721 ggagagacaa taattgatga caaaaattat tatttcaacc aaagtggagt gttacaaaca
     6781 ggtgtattta gtacagaaga tggatttaaa tattttgccc cagctaatac acttgatgaa
     6841 aacctagaag gagaagcaat tgattttact ggaaaattaa ttattgacga aaatatttat
     6901 tattttgatg ataattatag aggagctgta gaatggaaag aattagatgg tgaaatgcac
     6961 tattttagcc cagaaacagg taaagctttt aaaggtctaa atcaaatagg tgattataaa
     7021 tactatttca attctgatgg agttatgcaa aaaggatttg ttagtataaa tgataataaa
     7081 cactattttg atgattctgg tgttatgaaa gtaggttaca ctgaaataga tggcaagcat
     7141 ttctactttg ctgaaaacgg agaaatgcaa ataggagtat ttaatacaga agatggattt
     7201 aaatattttg ctcatcataa tgaagattta ggaaatgaag aaggtgaaga aatctcatat
     7261 tctggtatat taaatttcaa taataaaatt tactattttg atgattcatt tacagctgta
     7321 gttggatgga aagatttaga ggatggttca aagtattatt ttgatgaaga tacagcagaa
     7381 gcatatatag gtttgtcatt aataaatgat ggtcaatatt attttaatga tgatggaatt
     7441 atgcaagttg gatttgtcac tataaatgat aaagtcttct acttctctga ctctggaatt
     7501 atagaatctg gagtacaaaa catagatgac aattatttct atatagatga taatggtata
     7561 gttcaaattg gtgtatttga tacttcagat ggatataaat attttgcacc tgctaatact
     7621 gtaaatgata atatttacgg acaagcagtt gaatatagtg gtttagttag agttggggaa
     7681 gatgtatatt attttggaga aacatataca attgagactg gatggatata tgatatggaa
     7741 aatgaaagtg ataaatatta tttcaatcca gaaactaaaa aagcatgcaa aggtattaat
     7801 ttaattgatg atataaaata ttattttgat gagaagggca taatgagaac gggtcttata
     7861 tcatttgaaa ataataatta ttactttaat gagaatggtg aaatgcaatt tggttatata
     7921 aatatagaag ataagatgtt ctattttggt gaagatggtg tcatgcagat tggagtattt
     7981 aatacaccag atggatttaa atactttgca catcaaaata ctttggatga gaattttgag
     8041 ggagaatcaa taaactatac tggttggtta gatttagatg aaaagagata ttattttaca
     8101 gatgaatata ttgcagcaac tggttcagtt attattgatg gtgaggagta ttattttgat
     8161 cctgatacag ctcaattagt gattagtgaa tagataaaaa tatgttaaat atatcctctt
     8221 atacttaaat atataaaaat aaacaaaatg atacactaca taaagtgttc tatctaatat
     8281 gaagatttac caataaaaag gtggactatg atgaatgcac agtagttcac ctttttatat
     8341 ttctaatggt aacaaaatat ttttttatat aaacctagga ggcgttatga atatgacaat
     8401 atctttttta tcagagcata tatttataaa gttagtaatt ttaactatat catttgatac
     8461 attattagga tgtttaagtg caataaaaag tcgtaaattt aattctagtt ttggaataga
     8521 tggaggaatc agaaaagtag caatgatagc atgtatattt tttttatcag tagttgacat
     8581 tcttacaaag tttaactttt tatttatgtt accacaagat tgtatcaatt ttttaagact
     8641 aaaacatctt ggaatatctg aatttttctc tattttattt attttatatg aaagtgtaag
     8701 tatattaaaa aatatgtgct tatgtggatt accagtacct aagagattaa aggaaaaaat
     8761 agcaatttta ctagatgcaa tgacagatga aatgaatgct aaggatgaaa agtaagtaat
     8821 ggtagatata ataaagatat taacaaataa aaagtgttat ccaaataaga atagctgaaa
     8881 gttatcataa ttcatgaaac taataatgaa aacgagggag cagatgccaa gagacacaca
     8941 agtattaaat acatataatt tcgaagcaag tgttcattac tatatagatg acaaggtagt
     9001 atatcaaaca ttggttcaca aagatggtgc atggtcagtt ggtaaaatct attaagctac
     9061 attagttaca gatatcacaa actataatag ttaaacatag aaatatgtgt aaattgtgat
     9121 tgaaattatt caaaaacaca aaaatacgtg atgaaggaca aaatgatata gaaaataagt
     9181 atcaaacctt aataaatgat ttaattgata gtttaaaagt tataggaaaa atatataaag
     9241 aaataaaaac attaaaaaaa tataagatat gtttacaaat tactatcaga caatctcctt
     9301 atctaataga agagtcaatt aactaattga gtatctttaa attgaaatgt taggaagtga
     9361 tttaaatatg aaaacttaaa ttataaaaaa tcaatattaa tttattttta aaaaatagaa
     9421 aggagtgtat aagatttatt ttcaaagttt aaaaacaaga aaatcaattt aaatttcaga
     9481 aggaataaat gtggttatag aagtggattt attatcaaaa ataataatac taggaggttt
     9541 ttatgtcttt aatatctaaa gaagagttaa taaaactcgc atatagcatt agaccaagag
     9601 aaaatgagta taaaactata ctaactaatt tagacgaata taataagtta actacaaaca
     9661 ataatgaaaa taaatattta caattaaaaa aactaaatga atcaattgat gtttttatga
     9721 ataaatataa aacttcaagc agaaatagag cactctctaa tctaaaaaaa gatatattaa
     9781 aagaagtaat tcttattaaa aattccaata caagccctgt agaaaaaaat ttacattttg
     9841 tatggatagg tggagaagtc agtgatattg ctcttgaata cataaaacaa tgggctgata
     9901 ttaatgcaga atataatatt aaactgtggt atgatagtga agcattctta gtaaatacac
     9961 taaaaaaggc tatagttgaa tcttctacca ctgaagcatt acagctacta gaggaagaga
    10021 ttcaaaatcc tcaatttgat aatatgaaat tttacaaaaa aaggatggaa tttatatatg
    10081 atagacaaaa aaggtttata aattattata aatctcaaat caataaacct acagtaccta
    10141 caatagatga tattataaag tctcatctag tatctgaata taatagagat gaaactgtat
    10201 tagaatcata tagaacaaat tctttgagaa aaataaatag taatcatggg atagatatca
    10261 gggctaatag tttgtttaca gaacaagagt tattaaatat ttatagtcag gagttgttaa
    10321 atcgtggaaa tttagctgca gcatctgaca tagtaagatt attagcccta aaaaattttg
    10381 gcggagtata tttagatgtt gatatgcttc caggtattca ctctgattta tttaaaacaa
    10441 tatctagacc tagctctatt ggactagacc gttgggaaat gataaaatta gaggctatta
    10501 tgaagtataa aaaatatata aataattata catcagaaaa ctttgataaa cttgatcaac
    10561 aattaaaaga taattttaaa ctcattatag aaagtaaaag tgaaaaatct gagatatttt
    10621 ctaaattaga aaatttaaat gtatctgatc ttgaaattaa aatagctttc gctttaggca
    10681 gtgttataaa tcaagccttg atatcaaaac aaggttcata tcttactaac ctagtaatag
    10741 aacaagtaaa aaatagatat caatttttaa accaacacct taacccagcc atagagtctg
    10801 ataataactt cacagatact actaaaattt ttcatgattc attatttaat tcagctaccg
    10861 cagaaaactc tatgttttta acaaaaatag caccatactt acaagtaggt tttatgccag
    10921 aagctcgctc cacaataagt ttaagtggtc caggagctta tgcgtcagct tactatgatt
    10981 tcataaattt acaagaaaat actatagaaa aaactttaaa agcatcagat ttaatagaat
    11041 ttaaattccc agaaaataat ctatctcaat tgacagaaca agaaataaat agtctatgga
    11101 gctttgatca agcaagtgca aaatatcaat ttgagaaata tgtaagagat tatactggtg
    11161 gatctctttc tgaagacaat ggggtagact ttaataaaaa tactgccctc gacaaaaact
    11221 atttattaaa taataaaatt ccatcaaaca atgtagaaga agctggaagt aaaaattatg
    11281 ttcattatat catacagtta caaggagatg atataagtta tgaagcaaca tgcaatttat
    11341 tttctaaaaa tcctaaaaat agtattatta tacaacgaaa tatgaatgaa agtgcaaaaa
    11401 gctacttttt aagtgatgat ggagaatcta ttttagaatt aaataaatat aggatacctg
    11461 aaagattaaa aaataaggaa aaagtaaaag taacctttat tggacatggt aaagatgaat
    11521 tcaacacaag cgaatttgct agattaagtg tagattcact ttccaatgag ataagttcat
    11581 ttttagatac cataaaatta gatatatcac ctaaaaatgt agaagtaaac ttacttggat
    11641 gtaatatgtt tagttatgat tttaatgttg aagaaactta tcctgggaag ttgctattaa
    11701 gtattatgga caaaattact tccactttac ctgatgtaaa taaaaattct attactatag
    11761 gagcaaatca atatgaagta agaattaata gtgagggaag aaaagaactt ctggctcact
    11821 caggtaaatg gataaataaa gaagaagcta ttatgagcga tttatctagt aaagaataca
    11881 ttttttttga ttctatagat aataagctaa aagcaaagtc caagaatatt ccaggattag
    11941 catcaatatc agaagatata aaaacattat tacttgatgc aagtgttagt cctgatacaa
    12001 aatttatttt aaataatctt aagcttaata ttgaatcttc tattggtgat tacatttatt
    12061 atgaaaaatt agagcctgtt aaaaatataa ttcacaattc tatagatgat ttaatagatg
    12121 agttcaatct acttgaaaat gtatctgatg aattatatga attaaaaaaa ttaaataatc
    12181 tagatgagaa gtatttaata tcttttgaag atatctcaaa aaataattca acttactctg
    12241 taagatttat taacaaaagt aatggtgagt cagtttatgt agaaacagaa aaagaaattt
    12301 tttcaaaata tagcgaacat attacaaaag aaataagtac tataaagaat agtataatta
    12361 cagatgttaa tggtaattta ttggataata tacagttaga tcatacttct caagttaata
    12421 cattaaacgc agcattcttt attcaatcat taatagatta tagtagcaat aaagatgtac
    12481 tgaatgattt aagtacctca gttaaggttc aactttatgc tcaactattt agtacaggtt
    12541 taaatactat atatgactct atccaattag taaatttaat atcaaatgca gtaaatgata
    12601 ctataaatgt actacctaca ataacagagg ggatacctat tgtatctact atattagacg
    12661 gaataaactt aggtgcagca attaaggaat tactagacga acatgaccca ttactaaaaa
    12721 aagaattaga agctaaggtg ggtgttttag caataaatat gtcattatct atagctgcaa
    12781 ctgtagcttc aattgttgga ataggtgctg aagttactat tttcttatta cctatagctg
    12841 gtatatctgc aggaatacct tcattagtta ataatgaatt aatattgcat gataaggcaa
    12901 cttcagtggt aaactatttt aatcatttgt ctgaatctaa aaaatatggc cctcttaaaa
    12961 cagaagatga taaaatttta gttcctattg atgatttagt aatatcagaa atagatttta
    13021 ataataattc gataaaacta ggaacatgta atatattagc aatggagggg ggatcaggac
    13081 acacagtgac tggtaatata gatcactttt tctcatctcc atctataagt tctcatattc
    13141 cttcattatc aatttattct gcaataggta tagaaacaga aaatctagat ttttcaaaaa
    13201 aaataatgat gttacctaat gctccttcaa gagtgttttg gtgggaaact ggagcagttc
    13261 caggtttaag atcattggaa aatgacggaa ctagattact tgattcaata agagatttat
    13321 acccaggtaa attttactgg agattctatg cttttttcga ttatgcaata actacattaa
    13381 aaccagttta tgaagacact aatattaaaa ttaaactaga taaagatact agaaacttca
    13441 taatgccaac tataactact aacgaaatta gaaacaaatt atcttattca tttgatggag
    13501 caggaggaac ttactcttta ttattatctt catatccaat atcaacgaat ataaatttat
    13561 ctaaagatga tttatggata tttaatattg ataatgaagt aagagaaata tctatagaaa
    13621 atggtactat taaaaaagga aagttaataa aagatgtttt aagtaaaatt gatataaata
    13681 aaaataaact tattataggc aatcaaacaa tagatttttc aggcgatata gataataaag
    13741 atagatatat attcttgact tgtgagttag atgataaaat tagtttaata atagaaataa
    13801 atcttgttgc aaaatcttat agtttgttat tgtctgggga taaaaattat ttgatatcca
    13861 atttatctaa tactattgag aaaatcaata ctttaggcct agatagtaaa aatatagcgt
    13921 acaattacac tgatgaatct aataataaat attttggagc tatatctaaa acaagtcaaa
    13981 aaagcataat acattataaa aaagacagta aaaatatatt agaattttat aatgacagta
    14041 cattagaatt taacagtaaa gattttattg ctgaagatat aaatgtattt atgaaagatg
    14101 atattaatac tataacagga aaatactatg ttgataataa tactgataaa agtatagatt
    14161 tctctatttc tttagttagt aaaaatcaag taaaagtaaa tggattatat ttaaatgaat
    14221 ccgtatactc atcttacctt gattttgtga aaaattcaga tggacaccat aatacttcta
    14281 attttatgaa tttatttttg gacaatataa gtttctggaa attgtttggg tttgaaaata
    14341 taaattttgt aatcgataaa tactttaccc ttgttggtaa aactaatctt ggatatgtag
    14401 aatttatttg tgacaataat aaaaatatag atatatattt tggtgaatgg aaaacatcgt
    14461 catctaaaag cactatattt agcggaaatg gtagaaatgt tgtagtagag cctatatata
    14521 atcctgatac gggtgaagat atatctactt cactagattt ttcctatgaa cctctctatg
    14581 gaatagatag atatatcaat aaagtattga tagcacctga tttatataca agtttaataa
    14641 atattaatac caattattat tcaaatgagt actaccctga gattatagtt cttaacccaa
    14701 atacattcca caaaaaagta aatataaatt tagatagttc ttcttttgag tataaatggt
    14761 ctacagaagg aagtgacttt attttagtta gatacttaga agaaagtaat aaaaaaatat
    14821 tacaaaaaat aagaatcaaa ggtatcttat ctaatactca atcatttaat aaaatgagta
    14881 tagattttaa agatattaaa aaactatcat taggatatat aatgagtaat tttaaatcat
    14941 ttaattctga aaatgaatta gatagagatc atttaggatt taaaataata gataataaaa
    15001 cttattacta tgatgaagat agtaaattag ttaaaggatt aatcaatata aataattcat
    15061 tattctattt tgatcctata gaatttaact tagtaactgg atggcaaact atcaatggta
    15121 aaaaatatta ttttgatata aatactggag cagctttaac tagttataaa attattaatg
    15181 gtaaacactt ttattttaat aatgatggtg tgatgcagtt gggagtattt aaaggacctg
    15241 atggatttga atattttgca cctgccaata ctcaaaataa taacatagaa ggtcaggcta
    15301 tagtttatca aagtaaattc ttaactttga atggcaaaaa atattatttt gataatgact
    15361 caaaagcagt cactggatgg agaattatta acaatgagaa atattacttt aatcctaata
    15421 atgctattgc tgcagtcgga ttgcaagtaa ttgacaataa taagtattat ttcaatcctg
    15481 acactgctat catctcaaaa ggttggcaga ctgttaatgg tagtagatac tactttgata
    15541 ctgataccgc tattgccttt aatggttata aaactattga tggtaaacac ttttattttg
    15601 atagtgattg tgtagtgaaa ataggtgtgt ttagtacctc taatggattt gaatattttg
    15661 cacctgctaa tacttataat aataacatag aaggtcaggc tatagtttat caaagtaaat
    15721 tcttaacttt gaatggtaaa aaatattact ttgataataa ctcaaaagca gttaccggat
    15781 ggcaaactat tgatagtaaa aaatattact ttaatactaa cactgctgaa gcagctactg
    15841 gatggcaaac tattgatggt aaaaaatatt actttaatac taacactgct gaagcagcta
    15901 ctggatggca aactattgat ggtaaaaaat attactttaa tactaacact gctatagctt
    15961 caactggtta tacaattatt aatggtaaac atttttattt taatactgat ggtattatgc
    16021 agataggagt gtttaaagga cctaatggat ttgaatattt tgcacctgct aatacggatg
    16081 ctaacaacat agaaggtcaa gctatacttt accaaaatga attcttaact ttgaatggta
    16141 aaaaatatta ctttggtagt gactcaaaag cagttactgg atggagaatt attaacaata
    16201 agaaatatta ctttaatcct aataatgcta ttgctgcaat tcatctatgc actataaata
    16261 atgacaagta ttactttagt tatgatggaa ttcttcaaaa tggatatatt actattgaaa
    16321 gaaataattt ctattttgat gctaataatg aatctaaaat ggtaacagga gtatttaaag
    16381 gacctaatgg atttgagtat tttgcacctg ctaatactca caataataac atagaaggtc
    16441 aggctatagt ttaccagaac aaattcttaa ctttgaatgg caaaaaatat tattttgata
    16501 atgactcaaa agcagttact ggatggcaaa ccattgatgg taaaaaatat tactttaatc
    16561 ttaacactgc tgaagcagct actggatggc aaactattga tggtaaaaaa tattacttta
    16621 atcttaacac tgctgaagca gctactggat ggcaaactat tgatggtaaa aaatattact
    16681 ttaatactaa cactttcata gcctcaactg gttatacaag tattaatggt aaacattttt
    16741 attttaatac tgatggtatt atgcagatag gagtgtttaa aggacctaat ggatttgaat
    16801 actttgcacc tgctaatact cataataata acatagaagg tcaagctata ctttaccaaa
    16861 ataaattctt aactttgaat ggtaaaaaat attactttgg tagtgactca aaagcagtta
    16921 ccggattgcg aactattgat ggtaaaaaat attactttaa tactaacact gctgttgcag
    16981 ttactggatg gcaaactatt aatggtaaaa aatactactt taatactaac acttctatag
    17041 cttcaactgg ttatacaatt attagtggta aacattttta ttttaatact gatggtatta
    17101 tgcagatagg agtgtttaaa ggacctgatg gatttgaata ctttgcacct gctaatacag
    17161 atgctaacaa tatagaaggt caagctatac gttatcaaaa tagattccta tatttacatg
    17221 acaatatata ttattttggt aataattcaa aagcggctac tggttgggta actattgatg
    17281 gtaatagata ttacttcgag cctaatacag ctatgggtgc gaatggttat aaaactattg
    17341 ataataaaaa tttttacttt agaaatggtt tacctcagat aggagtgttt aaagggtcta
    17401 atggatttga atactttgca cctgctaata cggatgctaa caatatagaa ggtcaagcta
    17461 tacgttatca aaatagattc ctacatttac ttggaaaaat atattacttt ggtaataatt
    17521 caaaagcagt tactggatgg caaactatta atggtaaagt atattacttt atgcctgata
    17581 ctgctatggc tgcagctggt ggacttttcg agattgatgg tgttatatat ttctttggtg
    17641 ttgatggagt aaaagcccct gggatatatg gctaaaatat atgtttgata aaaaattatt
    17701 cctgtgctac taagaaatta tttttatata ataaatattg agatttaatt aaaagtcatg
    17761 tgttattgta atacatgact tttagttaaa atttttctat catttaataa tctattattc
    17821 ttgactattt tataataaaa ttcatatatg gaaatattaa tactaaataa ttaatagttg
    17881 ataaaaaata gataatatgc taaaagcaaa aactaattta gagccttgta actgtttatt
    17941 tgcaattata aaaacatctt taaacatatt gactataata taaaatatta actataatac
    18001 aaaacaatat taattaattt tctctacagc tatccctggt atagttatat ttccacccat
    18061 agttgattca taagttatag ttccagcact tatacctctt atagttatgt aatcatcctc
    18121 taatactctt gaaggagtta ttgatttttt ataactacat agtacaacct tatcataatt
    18181 tccagacaca gctaatctta tttgcacctc atcaccatct tcaataactt gaataacctt
    18241 accttcaaat tttacctttt tgtacttata atcatcaggt gttctagcta attggtcata
    18301 agtaatacca gtatcatatc ctttcttctc ctcttcttct tttttgcgtt gttcttcagc
    18361 ctttttagct tcttcagctt ttttagcttc ttcagcttta cgttgatttt cagcttcaat
    18421 agctttcttt tcgtcgtctt tcattttgaa ccatggttca gcatcagaca atttgctatt
    18481 taaagtttct atttctgttt tttgagaatc tataactgat gtttggttag aaatgacctc
    18541 ctcatggtct tcagaacaag ctggtgagga tatattgcca aaaaacacgc ctaaaataaa
    18601 tgccagtaga gcaataccct ttgtgctctt aaagaattta attattttct tagagcttcc
    18661 ttttccttca ttactaaatt cgttaccctc atttttttta gaaaacataa tacaatctcc
    18721 cttttattag atatatttta ttttaaaagt taaaaactta aaataactag ttgactaaaa
    18781 attatatatt catattttcc taaaatatat ttcccctaag cataatttta caaa
//
LOCUS       DAMJYX010000067        18804 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJYX010000067 DAMJYX010000000
VERSION     DAMJYX010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.067"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(280..672)
                     /locus_tag="PC479_002462"
     CDS             complement(280..672)
                     /locus_tag="PC479_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2805001.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1239..1601
                     /locus_tag="PC479_002463"
     CDS             1239..1601
                     /locus_tag="PC479_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805002.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1585..2310
                     /locus_tag="PC479_002464"
     CDS             1585..2310
                     /locus_tag="PC479_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805003.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            2330..2749
                     /locus_tag="PC479_002465"
     CDS             2330..2749
                     /locus_tag="PC479_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805004.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2739..3263
                     /locus_tag="PC479_002466"
     CDS             2739..3263
                     /locus_tag="PC479_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805005.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3575..4132
                     /gene="efp"
                     /locus_tag="PC479_002467"
     CDS             3575..4132
                     /gene="efp"
                     /locus_tag="PC479_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2805006.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4224..4679
                     /locus_tag="PC479_002468"
     CDS             4224..4679
                     /locus_tag="PC479_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2805007.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4842..5552
                     /locus_tag="PC479_002469"
     CDS             4842..5552
                     /locus_tag="PC479_002469"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2805008.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5606..6676
                     /locus_tag="PC479_002470"
     CDS             5606..6676
                     /locus_tag="PC479_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805009.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6704..10258
                     /gene="smc"
                     /locus_tag="PC479_002471"
     CDS             6704..10258
                     /gene="smc"
                     /locus_tag="PC479_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2805010.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10285..11565
                     /gene="ftsY"
                     /locus_tag="PC479_002472"
     CDS             10285..11565
                     /gene="ftsY"
                     /locus_tag="PC479_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2805011.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11668..12036
                     /locus_tag="PC479_002473"
     CDS             11668..12036
                     /locus_tag="PC479_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2805012.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12033..13385
                     /gene="ffh"
                     /locus_tag="PC479_002474"
     CDS             12033..13385
                     /gene="ffh"
                     /locus_tag="PC479_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2805013.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13423..13695
                     /gene="rpsP"
                     /locus_tag="PC479_002475"
     CDS             13423..13695
                     /gene="rpsP"
                     /locus_tag="PC479_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2805014.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13760..13987
                     /locus_tag="PC479_002476"
     CDS             13760..13987
                     /locus_tag="PC479_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2805015.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14095..14610
                     /gene="rimM"
                     /locus_tag="PC479_002477"
     CDS             14095..14610
                     /gene="rimM"
                     /locus_tag="PC479_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2805016.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14619..15314
                     /gene="trmD"
                     /locus_tag="PC479_002478"
     CDS             14619..15314
                     /gene="trmD"
                     /locus_tag="PC479_002478"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2805017.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15499..15849
                     /gene="rplS"
                     /locus_tag="PC479_002479"
     CDS             15499..15849
                     /gene="rplS"
                     /locus_tag="PC479_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2805018.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16170..17066
                     /gene="ylqF"
                     /locus_tag="PC479_002480"
     CDS             16170..17066
                     /gene="ylqF"
                     /locus_tag="PC479_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2805019.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17455..18747
                     /gene="brnQ"
                     /locus_tag="PC479_002481"
     CDS             17455..18747
                     /gene="brnQ"
                     /locus_tag="PC479_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2805020.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7953 a         1748 c         3472 g         5631 t
ORIGIN      
        1 aaaataaaaa atactattta aatagatatt aaaaaattga taccaagtat ttatttgcta
       61 tatgaataac aatttattta ggaactttac tttaacactg tataataata aagtaattgc
      121 taaaaacgtt tgatgatttg gatgtatttt tagaaattag aaaaatatat acttactgac
      181 taatgataat cttatatgta ttttagtttg agtgatttaa gaaaagtgtt ttgaatgggg
      241 attattttat agagtaaata aatatataat agtagaaaat tacatataaa gagtagtgtt
      301 tatttcatta ccttctttat ctgttccgat agggtcttgt agagatactt gagttttatt
      361 ttttttgtta gatctaatat tcattaaaat ctcattttca atacatctag aagcatatgt
      421 tgctaatcga gttcctttat ctatgttata agtttctata gctttaatta atcctattgt
      481 acctatggat attaaatcat cctgatcttc cgttgaatta ttatatttct ttgctatata
      541 agctactaat ctcatgtttc tttcaattaa tgtatctttt gcacttttat cgttttctat
      601 tttaaattta gttaaatact caatctcttc ttcaggggtt aagggttttt caaaagattt
      661 aagagctgcc aataggtcac ccccttttca acggtcactt taaattatat gaaggagact
      721 tatccaagtt gactgttatc taataaaata atatgcatat ttttagaatt taaaacatat
      781 atttgcaaac ttttgaatgg aatatgacat aaagagtggg aatttaaatt taagtttttg
      841 ttgtatatat atgaaataag gcgaagaaat tattgattta taaagtataa attggaattg
      901 aggtgtcaat aaatataaaa ggaaatagta tttatatatg ttttggtgtt gaagtaaaag
      961 attaatgcaa agcattagag tctataaaaa tatgaatata ggaggtgtaa ttttacaata
     1021 cttaaaaaag taaatgtaat taattgtttg tataaagttg attacaagca aaagtatcag
     1081 tcatagttaa tactgttaag ggtgaaggtg gtgtgttaat ttatatatgt atgggaagaa
     1141 attttgatta caatgaaaaa acttacaaaa gatatttatg aaaaaactta tagttaaatg
     1201 tttatatata aaatattatt taggggttgt ttttattatt gaaaataaga aagagtggtt
     1261 ttatatcaat tgaatgtata ataagtattg ctatattata tgtggctgtt tatttagttt
     1321 ctacatcatt gtataattgt tatagtttta tcagtagaaa tatatctgac agagaaatgt
     1381 taagtacagc aaaaaaatat atagaagatg agaagtatag aatacaaaat agtaagtatg
     1441 agttaattga agataagata gaaaaaaatt acataaatgg atatgaaatt aacagtaaaa
     1501 tagagcaaat tttagattat tatcaatgct atgaaataaa tatagagata aaaaatgaat
     1561 ttaaaaaact gaggtttaat agctatgtta ctagaaaata aaggctctat attaataata
     1621 acattgataa tattttctat tataagtact gtgtgcatta tgtgtatagg tttaatttat
     1681 acacataata atatgtttga gttaggatac aaggacattc aactaaaaga aatttcttta
     1741 agtggaatag aaatatcagt aagtaatgta ctttcttgtg ttgaggaagc tattgatgta
     1801 tctaataatg aagacgaatt tagagaatat ttcttaggaa ataatatgat tgaatgtata
     1861 aaaaaaataa aagatacatc ttatagtagt ttagaagaac tttctttaaa agtagaaaat
     1921 aatactattt atgacagagg agattttttt gaatttgata tagaaagtaa tgttagagaa
     1981 aaagaatttt taaaagcaat aaaagtaaga gtgaaaatca aaaatccatg gataggaatt
     2041 gacattgaag attatatgga taaatctaat aacaataaaa ttgatgacaa aacattcaat
     2101 aattataaag catctgaaaa taatgaaatt ctaaatcaat ttaaaaatga aagtgaaaat
     2161 ataaggaatc aagaggttgg tggatttaca gaagaagatg aagaagatga agaatatgaa
     2221 gaagaactta aacaaaacat aattgaaaat ataaagaaga attttgatga aaaaaaccta
     2281 gtacaggtat ataagtatag gggtgtataa ctttgaaaaa agaaggaatg tgactgtaat
     2341 agaattaata gtatctatca gtataatact cttgatagtt acattatctt ttccaaaaga
     2401 taatcttgaa aatcataaaa taaatttatt tgcacgtcaa ctttgttcag atataaggta
     2461 tgttaggcga attaatatgt ttagcaacta caatacatat atatattata taaatcaagg
     2521 taaacacgaa ggttatgtat tgagaaaaga tggtaaaaac attaaatcaa taatgctacc
     2581 taaaaatgca aaaatagcta ggagtattga aataataaag tttaaatctg atgggtcgcc
     2641 tcaaaaggcg tctacaatag agatatataa taatagatta agaaaaacta taacaataac
     2701 acctgtaagt ggaagagtac ttctgaaaga gggaaaatat gaaacataaa tatggttact
     2761 tgcttttgga aagtgtcgta tcattgtctt caatggtgat aattatacta gttttatatt
     2821 ctatattttt gtctacaata aacttaaagt taaaagtaga agataagata gaactacaac
     2881 aacaaagtct tgaaatcata aaatctatgg aaggaattat tagcaattct atgggaataa
     2941 tgaatgtatc taattatgaa gaaacattta aaaagacaac atctataaaa tgtagatatt
     3001 tagatgaaaa taataatgaa gaaggcataa gtaataaaga aattatattg aatgaaagac
     3061 gaaataagtt atttgtaaat tctttaaatg gcgaaagtag tcaagcaggt ggttatgaaa
     3121 ttggagatta tgtagatgag atgtatgtat caattactaa taatggtcaa tatgttaata
     3181 ttaaattgaa attatcaaaa cgtagtcaaa aatatgagac tgatttcaaa ataaaagtat
     3241 ggaattttag tgaaagtata tagaaataca taaatttaag agaaagaagt gacatcaata
     3301 agtcatataa aatataatta ttgagaacta tataactaaa taatatcaat atgtaaagtg
     3361 tatagattaa gaatatagag gataataata aaaatataga ataagtaatt tgccaaatac
     3421 ttgtttatat tgtataactt atgatattat gatattagta aaaatttgta atcctgactt
     3481 gacagaattc aagtaagaga tataatttta aaggcatagt aatgtaaaat ttactaaaaa
     3541 ataagaaatt tattttaata tggaggaaca aaaaatggta tcagcaggtg attttagaaa
     3601 aggtgttaca tttgaaaaag atggacaacc atgtttagta gttgattttc aacacgttaa
     3661 gccaggtaaa ggagctgctt tcgttagaac taaatacaaa aacttaaaaa caggagctat
     3721 aagagaagaa agtttcaatc caagtgaaaa attccctaaa gctgttatag atacaagaca
     3781 aatgcaatat ctatacaatg atggtgagtt atattatttt atggatcaag aaaattttga
     3841 acaaatacca ttaaactatg agcaagttga agatgcaatt aagttcttaa aagaaaatga
     3901 agttgctaca ataagatttt accaaggaca accattccaa gtggaagcgc caaactttgc
     3961 agagctagaa gttacagata cagagccagg tataaaaggt gatacagcaa gtaatgtaac
     4021 taaagcagct acagttgaaa ctggagcagt tgttcaagtt ccattattta taaatactgg
     4081 agataaagta aagatagata ctagaacagg tgaatattta tcaagagtat aaaaatttat
     4141 gtatataaaa gcttatatgt cttaataata taagatatat gagcttttag tttatatata
     4201 aaaaagaaag cgaggggtgt tacatgaaac atttatatga agaagttgca tacttaaaag
     4261 gcctagcaga aggactagaa ataagtgctg aaagtaaaga agggaaaatg atacataaaa
     4321 tagttgatgc attagaagta tttgcagatg ctattgtaac attggatgaa gaacaagaag
     4381 aacttcaaga ttttgttgaa tctatagatg aagatttagc agatttagaa gaagatattg
     4441 ctgacatgga agaagatctt tatgaagaag atgacgatga ggatgatgag gacttcagct
     4501 atatagaaat ggaatgccca aattgtggag agttagtaga aatagacgaa gatttgttat
     4561 atgatgatga agtagatgtt gtttgtcctg attgtaaggc tgtgatatta tcatcagaag
     4621 atgattgtga tgatgattgt acatgtggtg gttgttcaag ttgtgatgat agagagtaaa
     4681 aattcataag aattaaaact gcctatgatg cctatgagat attttgcttc ataggtagtt
     4741 ttatttttat gaggaagggt attatgtatt ttggttttgc aatacttgga caaaaatagt
     4801 acactagaat ttatagaata ggattttgga gggatgtaaa tatgaaaata agtaaaaaat
     4861 tactagataa tatacaaaga tttgaaaatg ttataaatta taaatttaaa aataaagaat
     4921 atatactaga agctcttact catagttcat attctaatga aaataaaaaa tataatttta
     4981 acgaaagatt ggaattttta ggggattcgg ttttaggcat agtgataagt gattatttat
     5041 ttaatgaaga agctaatctg cccgaagggg aactaactaa acttagagca aatatagttt
     5101 gtgaagattc attaagtgaa gtagctaatg atataaattt aggtattcac atgttattag
     5161 gtagaggaga agaagcaaca ggaggaagac ataggacttc aatattagca gacgcatttg
     5221 aagctgtaat agctgctata tatcttgatg gaggttttga aagtgcaaga cagtttatac
     5281 ttcatcatat ggaaaatata atttatgatt caagaaaagg aaatatattt agagactaca
     5341 aaacgcatct tcaagaggtg ttacaaggga atggagaaaa taatatctgg tacaggctta
     5401 tagaagaaaa aggaccagac cataataaaa gatttgtaat ggaagttgga attaatgacg
     5461 atgtacttgg tataggagaa ggcaagagta aaaaagaagc tgaacagtta gctgctaaaa
     5521 tagctttaaa gaaaaaatta tgggaaaaat aaatataata tgtaaaaata taaaaagaaa
     5581 tatttatatt agaaggatgt tgtagatgaa aaagagaatt atacctatat ttgttcctca
     5641 caaaggatgt cctcatgatt gcatattttg taatcaaaaa aaaataacag gtgtgagtac
     5701 tgatgttact agtgaagatg caagaaatat aattgaggaa tgtttagaga ctatagataa
     5761 agatgcagat gtagaaatag cattttttgg tggtagcttt actgctatag atattgatat
     5821 acaaaaaagt ttattatcag ttgctaaaga atatgtagaa aaaggtcttg taaaagatat
     5881 tagaatgtct acaagaccag attgtattga tgaagatata ttaagtatgt taaaagaata
     5941 taagacaagt ataattgaac taggagtaca atccttagat gaaaaagtat tattagatag
     6001 tataagaggt catcaatctg aaattgtata caagagttca aagatgataa aaaatagtgg
     6061 aatcaaactt ggattacaga tgatgattgg tcttccagct gacacagaag aaaagtgtat
     6121 atttacagca aaaaaatttg tagagctaaa accagattgt gttagagtct atccaacttt
     6181 ggtagtaaaa gatacaggtc ttgaaaagtt aatggaacaa aataaatata atccatttac
     6241 tcttgaagag agtgtaaata tagtaaaaaa agttcttgta ctgttttatg ttaataatat
     6301 caatgtaata agagttgggc ttcaagctac tgatgatata caaattggaa aagctgtttt
     6361 agcaggacca tatcatcctg catttagaga gttggtagaa gcagatatga taaaagatta
     6421 tttagaattt gttattttgc aaaataaaaa tataaagcaa atgttggtca aatctaataa
     6481 aaagaatatt tctaaaatca taggaaataa aaaaacaaat gttaaatata tggaagaaaa
     6541 atttggtgtg cttttaaaaa cacaagaatc agatttagat ataaatcaat tagaaattgt
     6601 gttagatggg aaaagtttaa ttagtgctaa tatgagagac attcatagaa aattgtatga
     6661 catttatgac ctttagtttt aataatgaag ggagagaaaa gatttgtatt taaaaagact
     6721 agagttaaag ggatttaaat cctttccagt aaaaacagat attattttta aagaaggaat
     6781 aacagccata gtaggaccaa atgggagtgg aaaaagtaat atatctgatg ctgtaagatg
     6841 ggtattagga gaacagagta ttaagagtct tagaggtgat aaactcgaag atgtaatatt
     6901 tgcaggtaca gatacaaaaa aacctatgaa ttattgtgaa gtggctctta ctattgataa
     6961 ttctgaaaat caattggaat tagattttac agaagtaact ataagaagaa gggcttatag
     7021 aaatggtgaa agtgaatttt ttctaaataa taaaagttgt agattaaaag atataaaaga
     7081 agtcttttta gatactggta ttggaaaaga tggttattca atcatagaac aaggaaaagt
     7141 agatgaaata ttaagtaata atcctttaag cagaagaaaa gtttttgatg aagcttgtgg
     7201 catatcaaaa tatagatata aaaaacagga agcagaaagg aatttaagta atacaaagga
     7261 aaatttagaa agaatagatg atgtatatat agaaattgaa aatcaattaa agcctctttt
     7321 taatcaacag acaaaggcaa aaaagtactt ggaaataagt gaaaaattaa agacacttga
     7381 agtaaatagc tttataagag aaattgaagg aatagaaaaa gaattaagtg aggttaatga
     7441 acatcgtaag gttattgaaa aggagctcaa tgaaaaagaa gaacaaaaaa atgttgtaga
     7501 gaaaaagcaa gaagatatta ataaagaggt agaggtatta caagatgtaa ttgaaaaatc
     7561 tgtagattat ataaattcaa ttaaaggagt tatatcaaaa aaagagtccc aaataaattt
     7621 aattaaggaa agaataagaa attttacgaa tgaaattagt agaaaaaatt tagaaataaa
     7681 ggatataaaa gagaagctaa atgaaaataa gcaatacata aaagaattag aaagcaataa
     7741 attatcaggt agtgaagaac tatctacctt gcaggaaaat ataaaagtat tggaaggaag
     7801 taaagataaa caaaaaatta agttagaatc cctaaataat gagatagaat tattgaaaga
     7861 aagtataatt gatatattaa ataagaaaca agagtttagt aataagctgt ctactttaaa
     7921 cgcaaataaa gaaaatatga atatcagaga tgaaaatata aattcagaga taacagagtt
     7981 aaacaaaaat attgaaataa aatcatctga attagatact ataaataaag aatttaatat
     8041 gcaaaatgaa aatttaaaga atgtaaataa tagacataaa gaattatcaa ttaatttgca
     8101 ggattcaatt agtgaacaca ataaactaga agatgaaata cagaaaagta aatataattt
     8161 aaatggatat aattcaaagt taaatgtcta tatagatatg gaaaatcatt atgaaggatt
     8221 taatagagga gtaaaagaag tactaaaaaa taaaaattta aaaggagttc atggagcatt
     8281 aggacagatt ataaatgtac ctgaaaagta tgagaaatct atagaagcag ctcttggtgc
     8341 ttatatgcaa aatataataa ctgataatga gtttagtgct aagtttgcaa taaactatct
     8401 aaaacagaat aatttaggta gagtaacatt tcttccatta aatataatta aatcaaataa
     8461 aataagtcta ggaaatttaa aagcaaatac taagttcata ggaatagcaa gtgatttaat
     8521 tacatttgat gaaaagtata gaaatataat tgaaaatata cttggaagaa caatacttat
     8581 aaataatata gatgagggca taaaatttgc aaaagaaact ggtcataggt ttaaaatagt
     8641 aactttagat ggtgaaatct taaatccagg aggttctttg actgggggaa gtctaaaaac
     8701 taatgggaat atactatcga gaaaaagata cattaatgaa tatacagaaa agataagtaa
     8761 tatcaagaat gaaatttcac atctagaatt aaaaagagaa tctttagaca aagatgtgaa
     8821 aaatatcaaa aatgaaattg attcacatga aagtaaaata aaagatttag aaaaaagtat
     8881 aataataaaa agtacaagta taaagaatgt tgagtctgaa attgaatcgt taaaaggtag
     8941 tattactaaa ttagaaaatg agaagaatga tttaaattct aacctaaatt atacacttga
     9001 aaaaagtgat gatgttagaa aagatatgga agagttagat gatttataca ataaaaataa
     9061 agaaaaaatt gatgcattga atgaggaaat aaaaagatac aatgatttat atgataaaga
     9121 aaaatctgaa tttgatgaat tgaacttaag tttagtgaag aaaacagaag tttacaatag
     9181 tatagttaga gatataaaaa gaatatctgg tgaaaattgt gagctagaag aaaaaaataa
     9241 acaattagaa gagtcattaa attatgaaga acatgaaata attaaattac aagattctat
     9301 actcactgaa gaaaaagaaa aagaaaattt aacaaaacaa ctaggggata gtaatagaaa
     9361 tcttgaaact agaaaaatag caaaagatga tttaaaaaat agttttgatg aaattaataa
     9421 agaattaaaa actatagata gacagcatat agaacttaaa gaaagtttat ttaaagttgg
     9481 tggaagatta gaaagattaa aaactagtca agacacatat ataaataagt tgtttgagca
     9541 atatgatatg accttagtac aagcattgga aattaaagat gaagatttgg atatagatag
     9601 aaaattctta gaaagtctaa agagagaaat aagaagtctt ggaaatataa atatagactc
     9661 tataaaggaa tatgaggaaa taaaagaaag atacgatttt tatagcgaac aaaagcaaga
     9721 tttagaagag tctatggaag agatagaaaa acttatacat actttagaag aaaatatgaa
     9781 atctgaattt gaaattaagt ttgaagaaat aagtaaaaat tttaaatatg tatataagag
     9841 attatttggt ggaggttgtg gagaattaac tattttagat aaagaaaatt tattagaaag
     9901 tgatatacta ataacagctc aacccccagg taaaaaaatg aaaaatttaa acctattatc
     9961 tggtggagag aaagctttaa cggcaataag tatattattt gctatattga ttacaaagcc
    10021 aacaccattt tgtatattag atgagattga agcgccactt gatgacgcaa atatatttag
    10081 atttggagaa tttttgaagg atttatctaa agagactcaa tttatatcag taactcatag
    10141 gagaggaaca atggaagctg ctgattatat ctacggagtt acaatgcaag aaaaagccat
    10201 ttcaaaagtt attagtttaa aattaaaaga agctcaggaa ataacagata ttatataact
    10261 tagattgaca taggaggaat aacagtgtta aaaaaattat ttggttttgg aaaagacaag
    10321 gaaaaggaaa tagaaaaaaa agatgctgaa gaagaaatag aagttgaaga cagtgttgat
    10381 aatttagaaa acttggaaga aactattttt tcaggacttg aagaagaagt gatagataaa
    10441 gtggaagata ttgaagaaaa aaatgaggaa agtgtcaata atgaaggcac tgaagaaatc
    10501 gaaaactttg aaaaaataga agttgagatg gatagtaatg gaaaagaagt agaaaatatt
    10561 tcaaataatg attctcaaga agagataatc gaagaattag aaaattatga tgaaattgag
    10621 tctaaagaga tagaagataa agaagataag aaagttaact tgtttgaaag actaaaacaa
    10681 ggattgacaa aagcaaagca aggaataact gatagaatag atgaagtttt aaaatcatat
    10741 acaaaaatag atgaagaatt acttgaagat ttagaggaaa ttttaataac agcagatgtt
    10801 ggtgtaaata caactatgga tataattgaa agactaagag ataaaataaa gcaaaaaggt
    10861 attactgaac caataaaggt aagagaagaa cttaaatcta tagttgaaga tattcttaca
    10921 aatgaaaact ctacactaga tatagaacca gcaccatgta taatattaat ggtgggtgta
    10981 aatggggtcg gtaagactac tacaataggt aaactagcaa atagatacaa aaaagatggt
    11041 aaaaaagttt tattagcagc agcagataca tttagagcag cagcgacaga gcaattagaa
    11101 atttgggcaa atagaaccaa tgtagacata ataaaacatc aagagggagc agaccctgga
    11161 gctgtagtat ttgatgctat aaaagctgca aaagctagaa aaacagatgt attaatatgt
    11221 gatacagcag gaagattaca caataaggct aatcttatga atgaacttgg aaaagtattt
    11281 aaaatagtag atagagaatt tccagaagca aaacgtgaag tacttcttgt ggtggatgct
    11341 acaacaggtc aaaatgcagt tgttcaagca aagacattta aagaagttgc agatataaca
    11401 ggtatagtac ttacaaaact tgatggaact gcaaaaggtg gagtagtcct tgcagtaaaa
    11461 tcagaagtag atgtacctgt aaaacttatt ggagttggag aaagtgtaga agacctacaa
    11521 gatttcaatg caaaatcatt ttcagatgca ttatttggta actaatgttc ttttaaaaat
    11581 aatggttgac aagagtgttg ttatgatata aaatacaata gtgtaaagta aataaccttt
    11641 acagtatact gctaggaagt gtaagatatg aatatagaaa aaatggtcga aattggattg
    11701 ttatttgaac agtataaaga attattaact gacaagcaga aagaaatagt tgctctatac
    11761 tatgaagaag attattcttt aggtgaaatt agtgaaaatc tgaatgtatc aagacaaggt
    11821 gtttatgata ccttaaagcg ttcagaaaaa atactaagag attatgaaga aaagttacac
    11881 ttagtatcaa agattcaaga gcaggaaaaa aatattaaga ttataaaaga taaaattatt
    11941 gacattaaag aagatttatt gcacaataga gattgtgcta atttaatccc taagctagaa
    12001 aatatagaag atgtatgtag ggagatgata aaatgatatt tgaaggattg tctgataaac
    12061 tacaaggggc atttagtaag ttaaaatcta aaggaaaact tacagaagta gatgtaaaaa
    12121 atgctatgag agaagttaaa cttgctcttc ttgaggcaga tgttaacttt aaagtagtta
    12181 aagatttcat aaaaaaagtt caagagagat gtgttgggca agaagttatg caaagcttaa
    12241 ctccagctca gcatgtaatc aaaattgtaa atgaagagct tacaagttta atgggagatg
    12301 tacaaagtaa agtaatgatt tcctcaaagc ctcctacaat tttaatgatg gttggattgc
    12361 aaggtgcagg taagactact acagcaggaa aacttggagg atattttaaa aagcaaggta
    12421 aaaaacctct attagtagct tgtgatattt atagaccagc agctataaag caacttcaag
    12481 ttgttggaga aaaattggat atacctgtat tcaatatggg agataaagaa aatccaataa
    12541 atattgcgaa agcaggttta agccatgcga ttaaaaatgc taatgattta gttattatag
    12601 atacagctgg tagacttcat atagatgaag tacttatgga tgagttaaag tctattaaat
    12661 cagaagttaa gccacatgag atacttttgg ttgtagattc tatgacaggt caagatgctg
    12721 taaatgttgc agaaagcttc aatgaggcac ttggtgttga tggagtagta ttaactaaat
    12781 tggatggaga tactagaggt ggtgctgcgc tttcaataag agcagttaca caaaagccta
    12841 taaaatttat ggggatggga gaaaagctag atgatataga acctttccac ccagatagaa
    12901 tggcatcaag aatattaggt atgggagatg tcttaagttt aatagaaaaa gcacaagaaa
    12961 atatagactt agagaaagct aaagaattag agcaaaaaat taaaaaacaa gagcttgatt
    13021 ttgaagattt cttacaacaa atggaacaaa tacaaaatat gggacctctt gacaagatac
    13081 ttggtatgat acctggtatg ggaaatgtaa aagaccaact tggagatgta gatttgaatg
    13141 gcaaagaaat gaagagaaca aaagccataa ttcagtcaat gactacatat gagagaagaa
    13201 acccaggagt attaaatgct tcaagaaaga aaagaatagc taaaggcagt ggaactagtg
    13261 tacaagatgt aaatagactt ataaaacaac ttaatgaaat gaaaaaaatg atgaagatgt
    13321 ttgctggttc acaaaagagt atgaagaaaa ggggcggctt aggcggttta ccttttttta
    13381 aataaaatat aaaaaagtaa aaattattcg gaggtgactg taatggcagt taaaataaga
    13441 ttaaaaagaa tgggagctaa caaaaaacct ttctacagaa tagtagtagc tgattcaaga
    13501 gctccaagag atggaaaatt tatagaagaa ataggatact ataatccaat ttctgaacct
    13561 aaacaagtaa gaataaatga tgaaaaagct ataaaatggt tagctactgg tgctcagcca
    13621 acagaagtag ttaaaaaatt acttgtaaaa aacggagtaa tagaaaaatt cgaagcttct
    13681 aaacaagcaa aataattttt tcttggcaat tagtttaagt aagggctcat tgctaaggtt
    13741 actttaggag atgaaatata tgaaagagct agttgtagat atagctaagg ctctagttga
    13801 taatcctgac tcagttgttg ttgaggaatt tgaagataat gatggtatcg tcttaaagct
    13861 tacagttgct caagaggata tgggtaaggt tattgggaag cagggaagaa tagctaaagc
    13921 tataagaact gtcgtaagat ctgttgcaaa tagagaaaat ataaaagttt ctcttgagat
    13981 agtataaaat aggaggatta ggtataatgc ctagtccttt ttatataaat gcgtagttta
    14041 agattttatg atgatatgac cacataagat taaatttttt aagaaggtga taacatgaaa
    14101 gaaaaattaa ctcattttaa ggtaggtcaa atagtaaata cacaaggttt aaaaggtgaa
    14161 gtaagagttt atccgcttac tgatgatata gagaggtttg atgaattaaa agatttctat
    14221 ctgggaaaag atttaaataa taaattggct gttgaaaaag taagatacaa aggttctatg
    14281 gttataatga agataaaagg catagacagc atagagaaag cagaaaaact aaaaaataaa
    14341 tttatgtatg tagctagaga agatagcaga gagcttgaag aaggcgaatt ttttatagca
    14401 gatatgattg gaatggaagt tttaactgta gatggaaaat atgtagggat actagaagat
    14461 gttcttcaat actcagcaaa tgatgtctat gtagtaaaag gtgaggaaga taaagaattt
    14521 atgataccag ctataaaaaa atttgtacct acaatagata ttgatgaaag aaaaatgatt
    14581 atagacccta ttaagggaat gatagattag gtggtaatat gagatttcat ataatgacac
    14641 tttttccaga aatatttaat tcgtacatgg atgaaagtat aatgaagaga gcagttgaaa
    14701 aaggtataat tgaggttcat atatataata taagggattt ttctaataat aaacataaaa
    14761 aagtagatga ttatccattt ggaggaggag cggggatggt aatgactcct caacctatat
    14821 atgacacata taagcatata ataacaactc ataatataaa taacccaagt gtaatttatc
    14881 ttactccaaa aggtaaggta tacaatcaat ctatggcaaa gcagatgtct ttaaaagaag
    14941 atataatact actatgtggt cattatgaag gaatagatga gagaatcatt gatttaatag
    15001 ttactgatga aatttcaatt ggagattatg tattaactgg gggagagctc ccagcactta
    15061 taatgataga ttctatatcg agattaatac caggtgtatt aaaccaagaa gaatcatttg
    15121 aagaagaatc atttaaagac aacttattgg aatatcctca ttacactaga cctagagatt
    15181 ttgaaggatt aaaagtacca gaagtgcttt tatcagggaa tcataagaag atagatgagt
    15241 ggagaagaga agaatctata aggataacca aagaaagacg atttgattta tataaaaaat
    15301 ctaatgaaaa ataaaaatat aatgaaaaat taatatatat gtatacaata aagtacctat
    15361 aaatggaatt aaaaacataa aagtgcttgt aataactatt gaagtatagt ataatataaa
    15421 atgttgaaaa tacgggcgtt cctctgttta cactggtcgg gtagttaaga acgtctatga
    15481 tatctaggag gaagaaagat gaatgaaatg ctaagagcaa ttgagcaaga acaattaaaa
    15541 aatgaagttc ctaattttgg acctggggac acagttaaag tacacgttag aattatagaa
    15601 ggaaaaagag aaagaataca agtattcgaa ggtgtagtat taaagagaca aggtggagga
    15661 gcaagagaaa ctttcacagt tagaaaaatg tctttcaatg ttggtgtaga aagaactttc
    15721 ccagttcact ctccaaaaat agaaaaaata gaagttacta gaaaaggtaa agtaagaaga
    15781 gctaaactaa actacttaag aggtagagta ggtaaagctg ctaagataaa agaagctaga
    15841 aataaataat taaaaacttt acttataaat ggggggctga tttatcagtc ctctttctat
    15901 tttaatgaaa attacagcaa ataattatac tagaaataaa tagaaatatt tgttgaaaat
    15961 atacattatt tatctatgaa tttatagctt ttaaaatgaa aaataaaatt ggtaaatatt
    16021 gcattatatt ttgtaacaat attaaaaata agagaacagt attatgtaaa gtatgacgtt
    16081 aaaaagatga caatattata gtttgttaag atagattttt taagtggaat taggatataa
    16141 taatataata ttaataagga gtgatacatg tgtcaaatga atacgaagag tatcttatga
    16201 atgacaatct acatataaac tggtatccag gtcatatgaa aaaaactaag gagttagtaa
    16261 aaaataactt aaaattgatt gatgtagtaa ttgaactttt ggatgctaga atcccgttta
    16321 gtagtaaaaa tccagacata gacaggcttg taggagataa gccaagagtt gtcgtactta
    16381 ataagagtga tatggcagat agggataaat tgaatcaatg gattgaatac tataagaaaa
    16441 ttaatatcaa agctatacca gtagatacaa taaaaggcgt tggtataaat aagataatcg
    16501 aagaatgtaa aaatgtaact agagaaaaaa tgagttcttt aaaagacaaa gggagaaaag
    16561 aaagagctat acgaataatg atagttggag ttccaaacgt tggtaaatcg tctcttataa
    16621 acaaattaac aggtagaaaa agcactcaaa ctggagataa gccaggagta acgaaaggga
    16681 aacaatgggt tagattaaag ggaaatcttg aacttttaga tacacctgga atactttggc
    16741 ctaagtttga agaccaagaa gttgcattaa atttagcatt tagtagagct ataaaggatg
    16801 agattttaga tactgaaact ttggctttaa gacttataga gaaacttatg aaaatagaac
    16861 ctgagaagtt gaaagccaga tataagttag attgtctagg agaaactcca attgaaacta
    16921 tggatatgat tggccataaa agagggttta taacaggtaa aaaagaacta gattatacac
    16981 gcatagccac aactgtatta aatgaattta gagatggaaa aataggaaat ataacattgg
    17041 aagttccaga aaacgttaaa agatagattt taaaacaaaa tcaaaaattt aatgttaaaa
    17101 atttaaaaaa gccttttagc aaaggctttt ttattttttt gaaatataga caaatatctc
    17161 catataaaag tataaattag gttagaaaac ctttaaaaat attaatttaa gacaaatata
    17221 cctaatattt tgaaaattaa aatgagtgtg attatttaaa attttagcat attgacttgc
    17281 ttgtttcgta taatatataa taagcacaaa taaatacaat atattaaaaa tttaatttat
    17341 ttaattttat taatttaata ataagttctt attgtttaat tcaacttact tatgcaagcg
    17401 taaacaatcg agacatttta ttaatttaat aaggttaaaa agggggattt tataatgggt
    17461 aagaccaagg atgttatagt atttggattt gcgttatttg caatgttctt tggagcaggt
    17521 aacttaatat ttccacctta cttgggaata attacaggtc cagaatggtt aattgccttc
    17581 ttaggattta cgtttgctga tgctggattg gcacttctag cagtaatggc tacagctaaa
    17641 ttcgatggaa acgtagttga aatgtttaaa agatgtggta tgaagttagg aattcttata
    17701 ggttgtgcag atattttatg tataggacca ttcttagcta taccaagaac aggtgcaaca
    17761 acttatgaaa tgggtataat gcctttgttt ggaacttcaa ttccagtgtt attattctgt
    17821 atactttttt ttgcgatttc atatgtacta acaataagac cttctaaggt cgtagatata
    17881 gttggacagt ttttaactcc agctcttcta atagctttag catttataat tataaaaggt
    17941 attatatcac ctcttggaga tatagtagat aaacctatga tacctaatgt atttgcagaa
    18001 ggtataggtc aaggatatca gacaatggat gcttttgcag caatagcact tgcatcagtg
    18061 ctaatagtat ctttaaatga taaaggatat tctactatta gtgacaagtt gaaaatgatt
    18121 ggtaaggcgg gtgttcttgc ttgtggagga ttagctctag tttatggagg attatgtttc
    18181 cttggggcaa ctgtatcaac aatgtatgga acagatgcag tacaatcaca agtaatagta
    18241 aatataactg aaggattgct tggaaatgtt gggaaagcga ttttagcagt agtagtatca
    18301 ttagcatgtt taactacatc tataggtctt acatctgcca caggtcaata tttctctaga
    18361 cttacaaaag gtaaattgag ttatgaaaaa atagtattag ctgtatctgt attcagtgca
    18421 gtagtagcat cttttggtgt tggaactata ataaagatag catctcctat actaagtata
    18481 gtttatccac catcaattgt tttaataatt ttagctttct tcaatgaaaa aattaagaat
    18541 gataatgtat acaaaggagc agtatatatg tcattgtttg taagtatact aacagttata
    18601 tctagttatg gagtagctgt tccagtagta aattctttac ctcttaattc attaggattt
    18661 aactgggtgg tgccagttat aatagctgga ataattggta attttatacc atcaaagagt
    18721 caatctaata cacttggaac aaactaagtt ttgtttaatt aaaatataat caattgtaaa
    18781 taaatgagtt aactaaaagt aaaa
//
LOCUS       DAMJYX010000068        18718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJYX010000068 DAMJYX010000000
VERSION     DAMJYX010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.068"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(210..1571)
                     /gene="rlmD"
                     /locus_tag="PC479_002482"
     CDS             complement(210..1571)
                     /gene="rlmD"
                     /locus_tag="PC479_002482"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2805021.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
     gene            complement(1598..2320)
                     /locus_tag="PC479_002483"
     CDS             complement(1598..2320)
                     /locus_tag="PC479_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2805022.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(2320..2649)
                     /locus_tag="PC479_002484"
     CDS             complement(2320..2649)
                     /locus_tag="PC479_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2805023.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            2979..3905
                     /gene="fba"
                     /locus_tag="PC479_002485"
     CDS             2979..3905
                     /gene="fba"
                     /locus_tag="PC479_002485"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2805024.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(4040..5671)
                     /locus_tag="PC479_002486"
     CDS             complement(4040..5671)
                     /locus_tag="PC479_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805025.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(6154..7191)
                     /locus_tag="PC479_002487"
     CDS             complement(6154..7191)
                     /locus_tag="PC479_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2805026.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(7210..7995)
                     /locus_tag="PC479_002488"
     CDS             complement(7210..7995)
                     /locus_tag="PC479_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2805027.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(8028..9161)
                     /locus_tag="PC479_002489"
     CDS             complement(8028..9161)
                     /locus_tag="PC479_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2805028.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(9209..10336)
                     /gene="hadC"
                     /locus_tag="PC479_002490"
     CDS             complement(9209..10336)
                     /gene="hadC"
                     /locus_tag="PC479_002490"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2805029.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(10336..11562)
                     /gene="hadB"
                     /locus_tag="PC479_002491"
     CDS             complement(10336..11562)
                     /gene="hadB"
                     /locus_tag="PC479_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2805030.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(11577..12377)
                     /gene="hadI"
                     /locus_tag="PC479_002492"
     CDS             complement(11577..12377)
                     /gene="hadI"
                     /locus_tag="PC479_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2805031.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(12416..13615)
                     /gene="hadA"
                     /locus_tag="PC479_002493"
     CDS             complement(12416..13615)
                     /gene="hadA"
                     /locus_tag="PC479_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2805032.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            14292..15290
                     /locus_tag="PC479_002494"
     CDS             14292..15290
                     /locus_tag="PC479_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2805033.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            15652..16179
                     /locus_tag="PC479_002495"
     CDS             15652..16179
                     /locus_tag="PC479_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2805034.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            16206..16802
                     /locus_tag="PC479_002496"
     CDS             16206..16802
                     /locus_tag="PC479_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2805035.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(16867..18450)
                     /gene="asnB"
                     /locus_tag="PC479_002497"
     CDS             complement(16867..18450)
                     /gene="asnB"
                     /locus_tag="PC479_002497"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2805036.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         5826 a         3433 c         2291 g         7168 t
ORIGIN      
        1 atattttcac ataaaaaata gatattaaat ttataaattt aatatctatt tctataatta
       61 aaactttttt tatttcgtat tcccacactc catatatata actacagaca catcaaaaca
      121 ccttttcaat tctttattcc taattttatt accctctaat ctaaatatta tatgtctatg
      181 aaactgtcta aagctcaaga tttctagcat tattttcgac gtagcaacac tacagtctca
      241 caatgtggcg tatttgggaa catatccata cacttaacca tcttaatctc ataaccatac
      301 cccttaaaat caaccaaatc aacaaccaaa gacttaggat tacaagaaat ataaacaatc
      361 tccttagcac caaatccaca aatatcccta atagcatcct tatgaatgcc tggtctagga
      421 gggtcaacaa taatcaaatc aggcttgtcc tttaaatcct taacaacctt agccacatcc
      481 ccagcaataa actcacaatt agtaagtcca ttcaacttag cattctcatt agcagcaaca
      541 acagcttcct caactatctc aataccatac actttcttag cagcaccagc cataacctgt
      601 ccaatagacc cagtaccact atacaagtca aatacaactt tatcattgta atctccaata
      661 aaatccctag caatactata aagaacctct gctcccttag tattggtttg gaaaaacgaa
      721 aatggtgaaa tcttaaactt aagacctaaa atttcttctt gtatataatc tcttccatac
      781 aatactctta actcatcaca ttggactgca tctgctaaac catcattaat agtatgaagt
      841 acacctacta actcagcttt taaatcaatc cctaaaagca tatctttaaa ctcatacata
      901 tcaaaatctt cttgagaaga agttactata ttaaccataa tctcattagt atgaatccct
      961 tttctaacta caagatgtct caaatatcct ttgtgattca ttgttctata ataaggaagc
     1021 tttttttcat tgaaaaactc tacactagaa actaatatct ttgagaagtc ttcatcaacc
     1081 aacatacact cctcaacagt ttgtatatct atatgctttc cttttttatg aagacctaat
     1141 gtcaaaggtc cattttttac ttcatctcca aaagtatatt ccatcttatt tctataatat
     1201 ttgttttctg gacttccttc tatccctaaa aactgaaatc caaataaatc ttgctttaaa
     1261 aataaatcca taacttgttt ttcttttatt tctaattgtt tttcatatgg cacagacaat
     1321 agtgtacatc ctccacactc tctaaagtgc ttgcaagttt cttcagtttc aagtggtgag
     1381 ttctctaata attctatcat cttaacttca gctttcttac ttctaacttt tttaactcca
     1441 gctctcacct tttgaccact tattccaccc ttcatatgga taactctatc tccaacttgg
     1501 cttaaagatg taccaccaaa ttccatttta tctacttcaa attctataac atcttttttc
     1561 ttcacagaca ttcctccaat ttactatagt atctttatta tctttcttca atcaatttta
     1621 cttcttcatc tgttaactct ctatactcac ctaactctaa actttcatcc aattttaaat
     1681 tgcccataga caatcttttt aagtatacta cttttttgcc tacgctttca aacattcttt
     1741 ttacttggtg aaacttacct tcatgtattg taagctctat ttctgattca tcatcacttt
     1801 ttaatatatt taactgtgat ggcatagttt tatatccgtc atcaagcact acaccttcta
     1861 aaaaagcttc tacatcttct tcagtaacta ctccatcaat ttttgcataa taagtcttag
     1921 gcacatgctt cttaggagat aaaactctat gagaaagctt tccatcatta gttaaaacta
     1981 ataatccctc tgtgtcttta tctaaccttc caacaggaaa tggttcaaaa gctaagtatg
     2041 ataaatcaat taaatctaat actgttgggt catatttatc tgtagttgct gatatatatc
     2101 catcaggttt attcatcatc aaatatatat attctctata tataacttct tctccattaa
     2161 ataatatctc atcattttca gtgtctactt gagttcctgg atttgaaact tcacttccat
     2221 ttactactac agacccttgt ttacaatact tctttatttc acttctactt ccataaccta
     2281 aatttgaaag aattttatct atacgttgct ttttagccat taaaaatgct ctccattctc
     2341 ataatatttt tctttttcaa atactgtaaa tgctaaatct atactttcta catctaaatt
     2401 atttcttata ctatcagtta ttattcttgc tactatatct tgtacttctt gtcctctatc
     2461 aaaccaagct acttcaacaa aaggatatac atctgctatt ttaccatctc ttattgcaac
     2521 tgactttata cattctattt caaagtagtc tcttggacat tttacagctt caactaaatc
     2581 atttatcatt ttttcactta ttttacatat atcattttca ttaattcctc ttatttttat
     2641 ctgtggcata gtttatctcc tttattttaa atttttagtg aattatcatc cacttaattc
     2701 ctttcgataa cgtttgaagt ggtaacttct tggtcaaggc aactattgtt atcagtatct
     2761 ccaaactaat aaggtagttc ctacccttat agttagcatt atatagctag ttttcctaaa
     2821 ttgtttatat tctttattat tttatttaat cctattgtct tctatttaac attatacttg
     2881 taatttatag ttattacaat ttactatata ctttttttga tttaacgtta taatataagt
     2941 gatgggtaaa ttttaaaagt taataggagg agtttaaaat ggcattaatt actacaaaag
     3001 aaatgtttaa aaaagcttac gaaggtggat ttgctatagg tgcatttaac ataagtgact
     3061 tagaacaatt acaaggtgtt ttaaaagctg ctaaagctaa aaattcttat gttatgatac
     3121 aagcttctat gtcagctgtt aaatatgctg gacctcatac attagttgaa atggttaagg
     3181 ctgcttctga tgaaataggt gtagatgtag ctcttcactt agaccatggt ccaaatatgg
     3241 atgctattaa aacttgtatt gatgctggtt tctcttcagt tatgatagat ggttctcatt
     3301 ttgactttga agaaaatgtt agaataacta aagaagctgt tgaatatgca cactctaaag
     3361 gtgttgttgt tgaagcagaa cttggtgttc tagctggaac agaagatgat gttacttctg
     3421 atgttcacaa gtatactcaa ccagctgagg cagttgagtt cgttgaaaga acaggtgttg
     3481 attcattagc aatagctatt ggaacttctc atggtgcttt caaattcaaa ggagaagcta
     3541 aattaagatt cgatatacta gaagaaatcc aaagcaaatt accaggtttc ccaatagttc
     3601 ttcatggtgc atctgctgtt gaccaaaacg ctgttgctac atgtaatgaa tttggtggaa
     3661 atatagctgg tgctaaaggt gtacctgtag atatgttaag aaaagcatct tctatggctg
     3721 tttgtaaaat aaacatggat actgacttaa gactagctat gactgctgct atcagaaaat
     3781 tcttagctga aaatccaaaa gaatttgacc caagaaaata cattggtgct ggtagagatg
     3841 ctatacaagc tgtagttgaa agcaagatag atgatgtttt aggttctgct aactctataa
     3901 actaatataa cttttaaata tttataaaag aaaagagatg acttaaagta attaaattta
     3961 tttttaagcc atctctttta agttgtataa attatgtatc ttataatatt ttaattatat
     4021 tgtcttttta aactgacagc taaacgtgaa tatgtccact ctttatcttt ctatggatgg
     4081 tagaaggata tatacctata acttcagcag cctttgttat tgaaccatac ttatttatag
     4141 ccttacttat cattatttgg tccactattt tatatgcatc attaagattc ataattccat
     4201 ttaccactat tggtgataaa tcatctgttt cattatcaat catatctaaa tttatcagca
     4261 tgttaaattc atcttctcca attacatttt tagctgataa tactacaaat ctttcaatga
     4321 tgttttttaa ttctcttatg tttcctggcc aagaatattc ttctaataac tcaataactt
     4381 taggcgaaac ttctacttct ctattgtact tttcattgta aagttttaag aaataattta
     4441 ctaatggtac tatatcctct tttctttctc taagaggagg tatttttact ggaataacac
     4501 ttaatctata gtacaaatct tgtctaaatt taaggttatt tctaaggtct tcatgagaaa
     4561 tattagtagc acttatatat ctaacatcta tttttatagg cttactacca cctattctag
     4621 taatagtatt ttcttgtata accctaagta attttttttg catctgcatt ggtaactcac
     4681 ctatctcatc caagaaaata gttccaccat tcgcctcttc aaaaagtccc tttttacctt
     4741 ttttcttagc tccagtaaaa gaaccttctt catatccaaa aaactctgac tctaataatt
     4801 catttggtat agctccacag tttatagcta taaaaggttt gtctctcctt gaactatact
     4861 tatatattgt tcttgctatt atttcttttc ctacacctga ttctccattt ataaatatag
     4921 aagaatctga ggctgcagct ctcttaacaa ctttttttat attgtccata atcttacttt
     4981 gacttatata ctctgtgtct tctgatatat cactatattt tatctgagat tcaagcttaa
     5041 taacccttga aatatctctc cctgttagta ctcctcctgt aagctttccc ttagaatccc
     5101 atcttggaac tgccgtatat gcaataagct tcccatctgg gtaaacgata tttctataaa
     5161 tctttttctt cactttaagc atgtttaata ttattggttc ttcaactaat ccttgtctca
     5221 caaggtctgt tatatttcta ccaagtacct cagttctttt aaaaggtaat atctcatcac
     5281 tgacagtgtt catcttttca attaccccat ctttgttaaa cacaacaaca acttcatcta
     5341 tatgctctat tatatcttca attactttat cagatattag agacttctca aataaataaa
     5401 ctatattatt ttcattatta ttatctttca tattgtatat atgtgcagtc atccttaact
     5461 ctctgaaagc tattttcttt ttactatctg tcagtatttc tgttttgtca aaatttgaat
     5521 ctaattcatt tatatttcta ggctttttta tatctagaaa actaaaaata ctatctatta
     5581 gatgattaca gtatattatt tcgcctgatt ctttacataa aatggctgga ttttctacat
     5641 gttctataag ccctattaaa ttattatcca ttaatattcg cacctttcat taagtaattt
     5701 attgaactac atacaaaatt tattgtttgc ttacaataaa taccatcagc acatataatc
     5761 aactccttat aatattcgtc ttttcaagta gtttatttca ttaaaacgta actaagatat
     5821 aattttaatg attatagttt gttaaaaatt tcattttgct tttaattgta ttttatcaca
     5881 tatttagtaa ttatttgtct atttaataca atttaattaa atttaagatt tatttaaaaa
     5941 aacaaagata atccttacag aatataactc acaatttgta agcaatatac atataaggat
     6001 tatccagatt atcttgtatt ataaaatttt attcaaacag actttttaat aactgacaaa
     6061 tacacactag caataactta tcaaagggtt aataattatg ttactaacta ataagactta
     6121 ttcaatcagt aatatttata tagtttgtat ctattaagct tttatagctt ctaataaatc
     6181 atcaacgcta tctaattttt ctataagcat tggaactatt tcatgtaagt ctccaactac
     6241 tccatagtca gcgatttcta aaattggagc tgctgggttt ttgtttatag caattatgaa
     6301 atctgatgat tgcatacctg ctaaatgttg tattgctcct gatataccac aagctatata
     6361 taggtttggt ttaacagtag ttcctgtttg acctacttgg tgagaatggt ctatccatcc
     6421 agcatcaaca gccgcacgag aagaaccaac tactccacct aatttgtcag ctaatttctt
     6481 aagcatttca aatccttctg gtccacctaa tcctagacca cctgatacta taacatttgc
     6541 atctgtaaga gatactaaat ctttcttagt tttaactgtt tctaaaacag tagttcttat
     6601 atcatcttga gttattttgt agtctaatgc tataacttca ccagttcttg tttcgtcttt
     6661 ttcagcttta tccataacac caggtctaac tgtagacatt tgaggtctat ggtttggaca
     6721 aatgattgta gccattatgt ttccaccaaa tgctggacga gtttgtttta ttttcttatc
     6781 ttctgggtct atttctaact tagtacagtc agctgttaat ccagttccaa ctcttgaagc
     6841 tattctaggt gctaagtctc tacctatatg agttgctccg aaaagcatta tttctggttt
     6901 tatttcatct attgcatctt ttattacttt tgtatatgca tcagttgtat atttttctaa
     6961 aagagcatca tctgcaacat aaactttgtc agctccaaaa gtgattaatt ctttagctaa
     7021 tccatcaaca tcttttccta ataatattgc acaaagttct gcatctactt ctttagctaa
     7081 ttttcttcct tctcctaata attctataac tactggagct atttttcctt ctctttgttc
     7141 tgcaaatatc catacgtttt tataagaact taaatctttt atatcattca tagtttgtac
     7201 tcctccaact tatataatgt gcttttgttt tagaccgatt attattttac tagctatttc
     7261 atcagcttta ccttctaaga tttctccttt tcctcttggt tcaggagtaa atgatctaaa
     7321 tactttagtt ggtgatgctt ttaaaccaac ttcttcttta tttacatcta aatcatcaat
     7381 tgtccaaact tttacatcat gtttatatgc ttttactatt ttatcaacag acatgtatct
     7441 aggtgtattt aactctttta ctgctgttaa aagtacaggt gtacttactt ttattaattc
     7501 gtatccatct tctaattgtc tttgaactat tatatctttt ccttctattt tgaaatcttg
     7561 tacatatgta acttgaggaa tgtctaattt ctctgcaatt tgtggtccaa cttgtgcagt
     7621 atctccatct attgcttgtc ttcctgcaaa tattatgtca tagtcaccta ctttagatat
     7681 tcccgctgca atagtatttg atgtagccca agtatcagct cctccaaatg ctctatcact
     7741 taataatatt gcatcatcag cacccatagc taaacactct cttagcataa agtctgcttg
     7801 aggaggtccc attgttataa ctgttatagt tacatcatca tgctcatctt taatttttaa
     7861 agcttcctct aatgcatttg catcatctgg gtttaatata cttggaactc catctcttat
     7921 aagagtacct gtttctttat ttattcttac ttcgttagta tctggaactt gttttacgca
     7981 aactaatatt ttcaaagtct atacctccaa tttcattaga ttatttatta ttttaatacg
     8041 tttgatgaaa ttaccatttt ttgaacttct gatgttcctt cgtatattga tacgattcta
     8101 gcatctctgt acattctttc gatttcataa tcttttataa atccatatcc accgtgaagt
     8161 tgtaatgctt tataagcaac ttcatttgca atttctgatg catagtattt agccatagca
     8221 gattcttgag caactctcat tcctgcatct ctcttttgtg ctgcatcata aactaatcct
     8281 ctagctgcac aaacttttgt ttccatgtca gctatagtaa attgagtatt ttggaattta
     8341 gctataggct taccaaattg aactctttgt tttgtatatt taacagcttc atctaaagca
     8401 ccttgagcta ttcctagagc taaggcagca acacctattc taccaacttc tagagtaccc
     8461 attgctatct taaatccttg accttctttt ccaagtaagt tttcttttgg aactttaacg
     8521 ttttcaaata ttaagtcaga agtttcagtt cctcttatac ccatcttatc ttcatgagct
     8581 cctgttgaga aaccatccca tttactttct actataaacg ctgatgttcc tctaagtcct
     8641 ttacttctat ctgtaactgc tattactata gctacatcac agaatggtcc attagttatg
     8701 aaagtttttc taccatttaa tatatagtgg tctccaacta attctgctgt tgtttgttgt
     8761 ccacctgcat cagaaccagc tcctggctca gttaaaccaa atgctcctat taattcacct
     8821 gttgctatac cttttaagta tttttgtttt tgctcttcag taccattgta aagtattggt
     8881 aatgaagcaa gagaagaacc agctgtaaca aaagtagctg ttgaagcaca tcttctagct
     8941 atttcttcca ttactatagc gtaagaaaca tatcctgctc ccccaccacc atattcttct
     9001 gggatattac tgcttataaa ttttgcatca gctaattttt tgattaattc cttaggcatt
     9061 acacctgttt tatccatttc tgctggtaaa gtatctaatt ctttactaac aaaatctctt
     9121 actgcttttc ttaaaagttc ttgttctttg ttatataaca tagcttcctc caattttcat
     9181 attttatagt aaatttattt taatgaactt acgctaaact catcatctca gcaaaagttt
     9241 gaatacgagt tctggcttgt tcattattct gagtttgttg gtcgatttca acatataaag
     9301 taggtattcc actatcttct atatcttttc taactaaagg ataatcgtat tcttctgggt
     9361 cacagaattt catcatacag aagataacac catctatatc tttcttttta acttcatcta
     9421 ctataagtga cccacgtttt ttcttagggt cataagctaa tgaacatcct tctatgtttg
     9481 accattgtct tgctaatctt tctaacgcat catcacctgc tggtacatct gttctgaatt
     9541 gtcttgtttc ttgtgctaag tcgtcagcta caactgatat attgttgtct tctaaaatgt
     9601 ctaatatatc tttagaatca gctaatatac ctgttaataa aactttcttt ccagaacaga
     9661 cttcttctgg catagcattt aattttgcta ttaaatcttt cactagctca gtatgttctt
     9721 cttttctcat aaagaaccca ctcttaatta ctaagcttct tattgatggt tttatagtat
     9781 tagaatgttt ataagctact tcaacaaagt ctctcatagt ttttctatgt tcattgtaaa
     9841 cttctatact ttcatgaatt tttgcttctt ctatttcata tccacaaatt tcttcaagtt
     9901 ctctttttac gcctttgtac tcactgatta agtattttac accagcttca agttttctat
     9961 tttgtgggtg tactaatgat atatatttta tatgaggtac tgctgatttc cagttttgtc
    10021 ctaaacaaat tagtgtatca cacatacctg gtataataac tccagataat tcatcataag
    10081 caccttttaa tccatattct aaacatgatt gcattattga acatgcaaat gcagggaaat
    10141 attgtttagc taaatctaat tctgtttgcc ctccccatat accaactgga agcattccag
    10201 ctgcatgtat aatttcttct gggcaataaa ctgggaaaca acctatagct tttttaccag
    10261 tttcactttt atattttttt acagccgcat ttggattttc aactacttct ttcattttag
    10321 ataaaatagc ttccattata tctcacctct attaagtttt tttctttctt ccattacttc
    10381 aactaaacct tgaattcttg tttcaaattg agcattagtg aaagctctag ggtctgcttg
    10441 gtcaccatca aatccagcat atggtaatcc agtttcttca gctgctcttc tttgcatttc
    10501 atattgtatt aaactcataa gtttacagct tctgttcata tgatagaatg ctccatcaca
    10561 tttaccctct actaaagaat caactctata ttttgtcata cggtctaggt ttacattgtt
    10621 aaacatagta ctatatgcta ctgccattcc atctaaatca ttaacttcat attgtaatgc
    10681 ccaagcatgt gggtaaacac tacctgtcat gttaactcca aatttagcta atgttttcat
    10741 cttgtatcct atatatggcc aacaaggtat accttccatc attattctgt atttttcttc
    10801 ccctctgaaa gaagatttac cagttttcat gttgtcctct aattcttcta taagtaactt
    10861 aaatgcttct gtagtttctt ttttacctct agcacaaact attacagcca tgtaagtaaa
    10921 taagtcaaat ccattcattg gagaaggaga agaatctgct ggtaaactca tagaatactt
    10981 ccatagtctt ccgttttcag ctgatatttt cattacttct tcaaacttct tagggtcaaa
    11041 tttctttcct gatataattt ctagttgttt tatagcttct tcaaattgag ctttaatata
    11101 atctattctt gattgagtaa cttcgtcttc attattgaaa gttgtatcaa tcattattaa
    11161 aggtatatct aattctcttg aaatattttc ataccatttt ataacttggt tacatatatt
    11221 gttacagcaa agtaggaaat ctggagctgg catatccaaa gcttcacatc caccattttc
    11281 taaaagacca aaatttgttc ttgcatatgc acataggtca atagaatatc ctttagattc
    11341 agctatttca cataattcta aagaaccttt tttagctgct actccagctg cttggttttc
    11401 tggatataat acgtttaagt caaatacttc tgctaactct tgaggaaata ctgatgttga
    11461 ccaacctaca ggtcttcctt cttctttagc tttaaatgca tttgcatatt gttcagctaa
    11521 taaatcatta attactactc tagcttcttt tttttcagac attttcatcc ctctttttat
    11581 atatttttca cttctttttg tgattctttt gcttcatcaa aagcatataa cgctgctcct
    11641 aaagcaccag ttaattgagg tatatcaggt acaataattt ctgtgttgat ttctcttgcc
    11701 atagctctta caataccact atttctagca actccaccaa ccataactac atttctttgt
    11761 actcctattc tttttactag gctagaaact ctctttgcta ctgaagtatg aatacctgcc
    11821 actatatctt caatttttgc attttcagat aaatgtgata taacctcaga ctctgcaaat
    11881 actgtacatg tactgcttat tgatacttca ttttgagaat tcatagatat acttccgagt
    11941 tcagatacat caacctctat tatttttgcc attacatcta aaaatcttcc tgtacctgca
    12001 gcacatttgt cattcataag aaagtttaat agtcttccat tattatctaa ttttaatacc
    12061 tttgcatctt gaccacctat gtcaataatg gttcttgtct ctggaattat gaaatttacc
    12121 cctctagcat gacagcttaa ttcacttatt tgcttatcag catctgaata gttcattctt
    12181 ccatatcctg taactacaac ttttttaata tcttctcttg caagacctgt cttgccatat
    12241 aatttttcta aaactcttga tggtccagta gtcccagtac cagaggatat tgtttcagaa
    12301 gctacaatat cttccccatt ctttaagatt actccctttg atgcagttga acctatatct
    12361 aatcccattg tgtacatctt tagcattctc cttttaaaaa atctagaatt actaattaat
    12421 atcttacaac tttactatct ttaaagttat ttattttctc ttcatcgtag cctaaagatt
    12481 ttaatacttc tacagtatgt tgacctactt ttggtgctgg tgtatattct ttaattcctt
    12541 catttctaaa cataactgga gtattaacta agacacctgt atttccgcta tcgtatgttt
    12601 tcttaaataa gaagtcattt gcccaagctt gttcgtcatc taataaatct tcacagcttt
    12661 gaattttttc aaatggtaag tctgcttctt ctaataaagc tgaccactcg tctaatgttt
    12721 tttctaacat agcttctcca actatcttaa ctaaatcttc aacatgatta accattgaat
    12781 ctatgttatt atatctatca tcttctaata tatattctct atttataacc ttacagaatt
    12841 tgcctaacca cttgttgtat tgtattaaag ctaattgaat ccatcttcca tctttacatt
    12901 tatatgtagt cattaatggg ctgtttggat tttctcttga taaaggcatt tcatttccgt
    12961 attgtgctgt tgttatcatt gttcccattc catatatagc tgtatggaaa agacttactg
    13021 ttactctctc acctttacca gtttgagctt ttttatgtaa tgctgctaaa cttcctgctg
    13081 ctagtgctag acctgcatag tggtcaccaa atcctgctgc tgtatttgct ggagatgttc
    13141 ctttttccat aacagattgg ctaactcctc ctcttgcgaa gtatgcagta tagtcaaatc
    13201 ctggtttatc ttttaaaggt cctttttcac catatcctaa tatttgagag aatattaatc
    13261 ctggatactt atcttttatt tggtcataag ctatacccat tttttctaat gcttgaactc
    13321 taacattagt tacaaatatg tctgcttctg ataataattt atgtaatatt tctactcctt
    13381 cttttgattt tacattaata cttacaccct ttttatttcc attttctaat tcaaacatag
    13441 ggttttcatc atctgatgct ggagatttaa atgttccacc cataactctt attccatcac
    13501 cttctatagg ttcaatctta ataacctctg caccccagtc acctaacatt tttgcacaac
    13561 atggtgctgc gatgaaactt gaaagttcta ctactttaac tccttctaaa agcataacgt
    13621 tttcctcctt aatagtatca attataaaat ttcgattaaa tcactcataa aatacttttt
    13681 tctgatatta actgtctgat gtcaaagttc ctaatcttat ctaaaataac ttaaaaatta
    13741 ttaaaattat tttttattgt tttattgtct tacacctata ttgttaagca tttatcgtgc
    13801 caactttata tttaatcaaa atatcgcttg atttgaatgt tttttaacaa tatgcaattg
    13861 aataatcttt ttcatatcgt ggcatcttgc cactatccat tgcattttgc tattctttct
    13921 atgcttataa aagttttttt tatatatttt tttatgaaaa agcttgacat ttcaaaaaaa
    13981 gttttcattg ctaaactcaa aaacacatgt tatcattgta attaatcagc aagttagtta
    14041 actaatttca aacttagata attttttaat aatatttaat ctaaaaaata acaatttctt
    14101 tgataaattt tagagtaatt attacattaa ataaaaaatt actaaatatc aaaataacac
    14161 agggaaaaca atttcaaaac taaattttta accttaaaaa ttttgtataa aaaatataac
    14221 cttatatgct acatattatt tctaaatgaa aaattaccat aataatttaa taattacagg
    14281 agggtattga tatgaaaata ctagtatttg gagcacgcga ttatgaagaa ccagtaataa
    14341 aaaaatggtc tgaagaacat aaggatgttc aagtggatat ttatcctgaa aacatgactg
    14401 aagaaaatgt agttaaagct aaagggtatg atggtatatc tatacaacaa actaactata
    14461 tagataatcc ttatatttat gaaactttaa aagatgctgg ggttaaagtt atagcttcaa
    14521 gaactgcagg ggttgacatg atacattttg atttagttaa tgaaaatgga cttatcgtta
    14581 caaacgttcc ttcttattca cctaatgcaa tagctgaatt agctgttact caagctatga
    14641 accttttaag aaagactcct ctagtaaaga aaaaagtctg tgaaggtgat taccgttgga
    14701 tagctgaact tcttggaaca gaagttagat ctattacagt tggtgttata ggtacaggaa
    14761 aaataggtgc tacttctgca aaattattca aaggcctagg agctaatgta attgcatttg
    14821 accaatatcc aaatagtgat ttaaatgata tattaactta caaagattct ttagaagacc
    14881 ttctaaaaga agctgacctt ataacattac atactccttt acttgaagga acaaaacata
    14941 tgataaataa agatactcta gctataatga aggatggagc ttacatagta aatactggcc
    15001 gtggtggttt aattaataca ggggatttaa tagaagcact agagtcagga aaaattagag
    15061 ctgctgccct tgatacattt gaaactgaag gattgttctt aaacaaaaaa atgaatcctg
    15121 gagaattaac tgacccagaa ataaataaac ttctttctat ggaacaagtt atattcactc
    15181 atcaccttgg tttcttcact agtacagcga ttgaaaatat agtttattct agtttaagta
    15241 gtgctgtaga agttataaaa acaggaactg ctactaatag agtaaattag tattttataa
    15301 atatattttt tcattaatta gctattatct aacaaaatag cctttaattt cccaaatata
    15361 agagagtcag ctcagtatat attgagtaga ctctcttata ttatttttta tattaaactt
    15421 gtactcttct tattcatacc aaattaaatt tatattaact tattctaact actcctactc
    15481 tatacatttt acctattttt acttttctaa ttatatacat ttgaatgggt acaatctgta
    15541 ttttacaaaa aaaacaccta acaaactatg aaaattgtat gattaatatt aatatattta
    15601 taagaaaaca taacaattat gttagctctg gatttttagg gagggagtat attggaaaaa
    15661 atgaataaag ttacttctaa aaaaactacc tatattgtta cttcagcatt atttgcatcc
    15721 ataatatgcc tgacaatagc atatatatta catattccag ttggtggaaa caatggatat
    15781 gttcatatag gagatgcttt tatttatcta gctgcaacta ttttgccaac aaactatgct
    15841 atagctgctt ctgcaattgg tgctggtcta gctgatttat ctacaggtgc tgctatctgg
    15901 gtaataccaa ctataatcat aaaaccaata cttgtattat ttttcacttc taaaagtgac
    15961 aagataataa ataagagaaa tatagttgct tcggttgtag caggtatagt aggattagtt
    16021 ttatacatgt ttgctgaagg tataattatt ggaagtttta caagtgcatt tgtaatgagc
    16081 ttacttggct tattacagcc aataggaagt tttattgttt ttataatctt aggtatggca
    16141 cttgataaac ttgattttaa aaaacgttat tttaattaag aatttaaatt tatggaggga
    16201 aaattatgaa tatcttatat acaattgaaa atagtatcta tgtaaatata actaatacat
    16261 gtccatgtag ttgtgttttt tgtataagaa acgaaaaaga tgaagttgca aatagtggta
    16321 gtttatggtt ggagcatgag ccaagtgtag atgaagtaaa agaagccttc aataaataca
    16381 atttagacga ctatgatgaa atcgtatttt gtggatatgg cgaaccatta atgagaataa
    16441 atgaattgat agagatagca aagtttataa aagaaaaaag tagcataaag ataagaataa
    16501 atacaaatgg tctaagtgat ttaatacata acaaaaaaac agctattctg ttaaaaaatg
    16561 ttatagatgc agtatcaata agtcttaatg ccccaaataa agaagcttat aatagagtta
    16621 ctcaaccaaa gtttggagaa aaatcatttg attatatgtt agattttgca agagattgca
    16681 aaaaatatat aaaagaagta gctttttcag tagtagatga aatatctcca gaagaaatag
    16741 aagaatctaa acaactggca aaaaaacttg atataccatt aagagttaga cataagaatt
    16801 agcaagtaaa attataaaac attaagaatc acctgaatgg tatttcaggt gattcttcct
    16861 cttgtattaa cattttacaa aatagatttc ataccacact aggaatgtgt aaatcatcca
    16921 aatctttctc caattatcag agtttccact aatgtagtca ttaaaaatct cattaatctc
    16981 atctatgtta aagaatttat ctgctatctc actattgaat ttatctcgga tatccttatt
    17041 atatcggtca tcagccatcc atatacgaat tggcacaata aatcctaatt tatttctaaa
    17101 agcaatctct tctggtaatg tttttgatgc agctgtacga aaggcaattt tattctcttt
    17161 tcctttcact ttatattctg atggtattct ggatgcaata tcaaatattc ttgtatctgt
    17221 aagaggcatt ctgacttcca atcctactgc agtactcatt ttatctatat taaaataaat
    17281 atctccttcc aaccatattt gaatatctac atttgacatg ttagtaagtg tatctagttc
    17341 ttctgtttct tcataaattc cttttaccac ttcaatagga agcacatcag gattgtattt
    17401 cttaagaatt tttcttttct caccctcatt cataatgttt gtatttccaa tataaaatgt
    17461 cttcaaattc tctccataac actctgcctt atggtatata ttatatccac caaaaaactc
    17521 atctgctcct tctccagagt aacagatatt tgtgttttca gctgctgcct tgcatccaat
    17581 agcaaaaacg atagcagatg catctccagt tggttgctcc atatggtaca ttacataagg
    17641 tactgacgca aaatactctt caggtgtaat tatatatctc gaatttttac acccaagaat
    17701 attagctgtc tgttgtgcta attttgattc atcaaaacgc tcttcctcat aaccacatga
    17761 acctgttttt tctatatctg acattgccaa tatataagat gaatcaacac ctcctgatag
    17821 gaataactct gcagtttcat tctctgattt tacctctggc ataattttct gaagtgttgt
    17881 atgaatttca tccgtccatt cctcaataga cttgctgtgg tcaggatgaa attcaggttt
    17941 ccaataacga ccaatacata ctgttttatt ctgatagaca aggtaatgtc ctggcataag
    18001 cttctttact ccacggaaga atgtattttc acctgctaca tatgtgaaag acatataaat
    18061 ctgtaacatg tcttcattta actctttcac aaatcctggt tgctccataa tcttacgaat
    18121 agatgaacca tagagaagct tcccattttc agtctcataa taatagaaag gttttacccc
    18181 aaactggtct cttaggcaga agagtttctt ttccacagta tcccataatg caaaagcaaa
    18241 cattccatgc atgtgatttg ccatatcata tccccatgtc ttatatgctt ccactaaaat
    18301 tgctcgttca cgctcatctc tacataatga agaatcgatt ccaagttctt cacaaagaac
    18361 tttccagttt cgaatatgcc cattgtattc tagaattttt atcatatatg tgtcttttcg
    18421 tgttaaatac ttttcttcta aatttatcat atgtgtatcc tttcgtatta aaacatttaa
    18481 cttctaaatt tattaaatgt ttatatatat ttattcactt ataatcactg aaaaatcgac
    18541 ttttttcaaa gtaaattctt tttgaattag tactaatctg ctctttatta atggcataaa
    18601 tatgattatc aagtattatc agtgttataa cattgaattt tttattcttc ataaatattg
    18661 ccccctttat attaccaact aaatataata tatcttatgt tacttacata ttctttaa
//
LOCUS       DAMJYX010000069        18664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJYX010000069 DAMJYX010000000
VERSION     DAMJYX010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.069"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(135..2147)
                     /locus_tag="PC479_002498"
     CDS             complement(135..2147)
                     /locus_tag="PC479_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805037.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2618..3268
                     /locus_tag="PC479_002499"
     CDS             2618..3268
                     /locus_tag="PC479_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2805038.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(3408..4784)
                     /locus_tag="PC479_002500"
     CDS             complement(3408..4784)
                     /locus_tag="PC479_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2805039.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(4800..6713)
                     /locus_tag="PC479_002501"
     CDS             complement(4800..6713)
                     /locus_tag="PC479_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2805040.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            6849..7481
                     /locus_tag="PC479_002502"
     CDS             6849..7481
                     /locus_tag="PC479_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2805041.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            7620..8159
                     /locus_tag="PC479_002503"
     CDS             7620..8159
                     /locus_tag="PC479_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2805042.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(8335..8505)
                     /locus_tag="PC479_002504"
     CDS             complement(8335..8505)
                     /locus_tag="PC479_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2805043.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            8790..9857
                     /locus_tag="PC479_002505"
     CDS             8790..9857
                     /locus_tag="PC479_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2805044.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            10027..11928
                     /gene="cooS"
                     /locus_tag="PC479_002506"
     CDS             10027..11928
                     /gene="cooS"
                     /locus_tag="PC479_002506"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2805045.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            11921..12394
                     /locus_tag="PC479_002507"
     CDS             11921..12394
                     /locus_tag="PC479_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2805046.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            12369..13595
                     /locus_tag="PC479_002508"
     CDS             12369..13595
                     /locus_tag="PC479_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2805047.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(13734..14921)
                     /locus_tag="PC479_002509"
     CDS             complement(13734..14921)
                     /locus_tag="PC479_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2805048.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            15194..16018
                     /locus_tag="PC479_002510"
     CDS             15194..16018
                     /locus_tag="PC479_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2805049.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            16147..17412
                     /gene="gluD"
                     /locus_tag="PC479_002511"
     CDS             16147..17412
                     /gene="gluD"
                     /locus_tag="PC479_002511"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2805050.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            17625..18215
                     /locus_tag="PC479_002512"
     CDS             17625..18215
                     /locus_tag="PC479_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2805051.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         6990 a         2280 c         2887 g         6507 t
ORIGIN      
        1 ttatcctatt cctattaatt cctattattt atgtacaaaa taaattataa tttcagggta
       61 cttgaaaaat aaaaaaacct attgaaggag tactttaatc ctgaatagat ttctttgttt
      121 gataaattat aattttatag acctttatat tttaatttat tgtaaaaatc ttctcctttt
      181 tgagttattt tacttccata tcttcctgaa ctacaaatta taagttcttc tttttttaga
      241 tattcaaata tttttttaat ttttccttct gtaatttgaa ggttcttatc gagtagtgct
      301 tttaaaatta atcctctacc aataccaaca tctgattcct tatttaaaat tagtatctct
      361 aatatattca atatatcatt cttactatat tttagtttta atgatgtatt tttgttatct
      421 gaagacctta agtttggagg taaatctttc tctaatacaa tatcttcaca cataatgttt
      481 aaataggatg taacattttg gagttctctt atattacctt tccacatata gttttgtata
      541 agtttttttg cttctggtgt gattactaat ttcttattaa ttaaatcttc cataataatt
      601 aatatgtctt cttttctttc tcttagagga ggtatattta ttggaattgt atttaatcga
      661 taatataaat cctctctaaa ttggctatta tcaatcattt gttctaaatt cttatttgta
      721 gcagatatga ttctaacatc tatattaatt acattgtgag acccaacagg cattatctgc
      781 ttttcttgaa gaactctaag tagttttgtt tgtaattcta aaggcatatc gcctatttca
      841 tctaagaata ttgttccatt attagcaagt tcaaataatc cttgttttcc atcttttaga
      901 cctccagtaa aagttccctt gtcatatccg aatagctggc tttctaataa attttcagga
      961 acggctgcac aatttactgc tataaagggt tgattttttc tatttgagtt gttatgtata
     1021 gactgagcta tgagttcttt accagttcca ctttcacctc tgataagtat gcttaagtca
     1081 gatttagaaa ccttcttagc taaatctata cattcaaaca ttttaggact atttgttttt
     1141 atatccttaa atgtatattt tgctatttgt cctttttctc ttaatttttt agtgagattt
     1201 tgttctaatt tttttatgta tgttatttct tgtaaactat aatatgtgcc cattttttca
     1261 tctctattgt atatgttttt tttatttaca tttatatatt tcttattgaa tacgacaact
     1321 ttatctagta tctctttctc actcaataaa acttttaaac tatctacaaa tatatcctca
     1381 atgtattttc catatatatc ctcatttatg tctaaaatat cttttacttt actattatat
     1441 gtatttattt caccatcttt tgaagtaaat aatataccat cttttgataa attcaatatt
     1501 gtatctaatt cttctatttt taaaaataat tccttatatt tatcttttat accagtatct
     1561 aaacttatta tttcttctga gtatttaact agattagatt gtatttcctt tgaatcaatt
     1621 tgtaataaat ttattatttc tataaatgta gaaatatcta tatatctgtg acctaaatca
     1681 aatatatctg agataaagct aggtactttt tctggaactc ctggagttat agctattttt
     1741 atatttttat agttattatc attcatatat ggaattggtc taatattagt cactccaatt
     1801 ttataaaata aacttatagt ttctagagta gtttcatcag aatcatttac gattagaaca
     1861 tttgtaccct gtggtaaaga tagtagatta taaattttat cttccctaaa agttcttctt
     1921 accacaatca cttttttctt atctagtata ttatttataa ttttgtctaa tctttctact
     1981 gacattacta atatcacatc atcatttata atatcattat catttatttc atttatgaag
     2041 tatctattaa tatttataga atctccaaaa actaatttca aattattttc taaaaagtct
     2101 attaaatctt ttctactatc atttaccaga gctactgatt ttttcattta tttctcctta
     2161 tataataaat agtttccaac aaaatagttg tataaagcaa tataattttg ataatttaaa
     2221 tttatcaaat aaaaaagaaa gtgtctataa tgactttata aaagttgcat taagacactt
     2281 tctttcttat aattaaaaat cttgtcacca aatcccccta attgttgctt aaaacttaaa
     2341 ctgtatgtat atttttcatt tctattcgat taaattataa tatacaccca aaaaaattta
     2401 ttttaataaa ttctattgat aatataattt tgtaaatata gaattatatt aaaataaatt
     2461 atatattaaa atatatatgt ataagtttat tctgcgtcag tagaatgaat ttgtcaagtt
     2521 taatttagtt gtaattttat cttttattaa aaataatata aattaaaaca tataaataaa
     2581 atattttata caatatattg taaaggaggt aactttgatg aaagcagaaa aaacaagtat
     2641 atatccaatt attaaaaata atttaaacaa aatatcaatt gttgaatata aaaagggtca
     2701 aagatttgta gcatcagata aagacttaca agaagtgttc tttataatag agggagttgc
     2761 attagtggag tgtactacta gaggaggaaa taaatttctt gttgacattg tacctgaaaa
     2821 tgaatttata ggcaaaatta gttacatata tgaacataat ttgaaatgtg atatatttgc
     2881 aaagaccaat ataaaattat ttcgatttga aaaaaatgta tttgaagagt tttatctcaa
     2941 accagatttt attgcactat ttcacagaaa atgtacaaga agaatatatg aattatataa
     3001 aaccagaatg gtaagagaac tattttcttg cacagaagtt attgcctatt gtatatcaaa
     3061 taaacaagaa aataatgtct gcaatataaa agctattcgt aatttcagtg aaatctattc
     3121 aattagtaga aaaagtagat accattctct gctaaagtta actgaaaagg agatagtaaa
     3181 aaaaatgggt aatacatatg aaattttaaa ctatgatgaa ttacataaat tggcatttga
     3241 agtaaaagaa tttttggaag atgagtaaaa attgttactt aatcaaaata aagtataata
     3301 atttctgtaa aaaatagaaa tagtctcact gaaaatcaaa aatactttag ttattttcaa
     3361 tgagactgtt tagaaatatt gtatacttat aatgtatagt ataattatta atcaatttcc
     3421 tttactatat taatagccca ttttttagat aatgctctag gaacaccaaa tataaactta
     3481 accacttctt cactataagt gagcaaaacg agtacaaata taggtgcacc tttaaatgca
     3541 gctataaaag ttagaggaat agaagctaac cacatacatc ctaattctaa aaatagagca
     3601 tatttagtat ctcctccact tcttaaaata ccaattataa ccaatgtatt aaaagattta
     3661 agagccatta gtattcccat tataaagaat atcttagtta tatcagaagc taagctatca
     3721 gaaacactaa acatttttaa taataatggt atatttaaaa ttaaaataat tccaagtatt
     3781 aaaccagctg aaaatacaag tatagaaaac tttttagcat attcaatggc tcttttaata
     3841 tggtcagctc caagttcatt tccaagcata atagatgcac cagatgcaat acatacagct
     3901 gtcattatga agaaattacc tgtactggtt gctatctggc tagctgctat agcagaagta
     3961 ccagctcttg catatgcaac agaatacagg acagttccaa ttgcccatac agagtcattt
     4021 acaaatatag gaaagctttt tgatgaaaaa gattttataa aaattttatc aatagctttt
     4081 agatttttta gaccaaactt taatatatag tcttttttat aaaggtaaac ataagtaatc
     4141 attaaaatta attcacatat tctagcaata acagtagcta gagcagcacc ttttacaccc
     4201 aatgcaggaa gaccgaagtt accaaatata aatccataat ttaaaataac atttgttaca
     4261 agtgcaaatg cactacaaat catacctaac ttaggatttc taacacctct tgaaccagtg
     4321 ctaaatacag tacttacagc aattaatgga taactaaata ctgcaatact aaagtaatct
     4381 atacaaagtt ttaccacctc agaatcatac gaaaatatgt gtataattgg ctttggtgtt
     4441 agtaaagcag gaataacaaa tacaaaactt aaaacaattg ccaatacaca agtaagacct
     4501 gtgacttttc ttatattact cacatccttc tttccataaa actgtgaaat aaaaactcca
     4561 gcacctcctg tgataccaga taaggacata ttaaaaagaa agaaaaattg gtttgcaact
     4621 ccaactgaag ctacagatgt ttcccccaaa ctacttatca taattgtatc tattacattt
     4681 acagaagtag aaattaagtt ctgtatgata ataggtatac ataaagatag aagtatttta
     4741 taaaatcttt tattttcgtc ttttaaagaa aatagttctt gcaaataatc ctctcctttt
     4801 tacataaatt cttcccactc atcatataga gaggcaaggt tattttctaa attaattttt
     4861 tcttggctaa cttctttagc tttatctgga ttagagtata cttcttcttg acataaaagt
     4921 atatctaatt tttcaatttc aatctctaac ttttcaattt ctttttctat attttgtctt
     4981 tttattctat ttttcttttc tatttctctt tgttctctct cttttctttt ttcatctttt
     5041 atttgagttc tagttttttc ttctttttct tctataatac tcatctcttg gatttgcctt
     5101 tttttatcta tataataatc ataatttccc agatattcag taattccatt ttcatctaaa
     5161 actaaaattt tatcaacaac agtatttaga aagtatctat catgtgatat tgtaaatata
     5221 gtcccatcat aattagttaa tgcctcttca aggacttcct tagaatcaat atctaggtga
     5281 ttagtaggct catctaataa caataagttt gcatttgata gtataagttt aagtatggca
     5341 actctagctc tttctccacc actcaatgta gaaatcttct taaaaacttc ttcatctaca
     5401 aataaaaatg cccctaacat atttctcaaa gtagtttgag taagatgagt attattttcc
     5461 caaatctcat caattattgt gttatctaaa ttaagagttt tttgttcctg atggaagtag
     5521 gaaacatgta cattagttcc aaatttaata ttgccactaa gaggttgtag ttcattagtt
     5581 attattttaa ataatgtaga tttgccaaca ccatttgcac ctataagtgc aactttttcc
     5641 cctctatata tatctaaatc caagtcttta aaaagtattc tttctccata acccatagat
     5701 acatctctaa tttgaagaac atcattacca ctctggacag aaggcgtaaa ttgtatacga
     5761 gcttttttcc tataagcttc tggtttatct aaaacgtcta ccttgtctaa tgctttttct
     5821 ttacttcttg cacgctttag gtgtttttct ctaccataag cttttagtct ttcaattgat
     5881 tcttcttgct tttttatttc tttttgttgg tcttcaaatt tcttaagctc tagttctttt
     5941 tctattttag ataactcaac aaatttagag tagttaccat tatatacttt aagttttttg
     6001 ttgtgaactt caaaaattct atttacggat tggtctaaga aatatctatc atgagatatt
     6061 aacatgacag tccctttata ttgttttaaa aatacttcta accattctat tgcttcagag
     6121 tctaagtggt tagtaggttc atcaagtaac aatagagttg gtttcttaag aagtaatttt
     6181 cctaaaagaa ctctagtttt ttcaccacca gaaagtatgc ttattggctt atccatatca
     6241 ttgtcactaa atcctaatcc ctttagaact cctttggctt cagatttata tccatatcca
     6301 tttaaatctg aaaataattc tagtttatgt gaatattcat ccatgatttt ttctataatt
     6361 ggagagtttg tttttgtact ttcttcagat attttaacct ccaattctct taaataggat
     6421 tccatttcta tcaaattttt aaaaacttct agtacttctt ccaaaatagt attatccgaa
     6481 taaaaattgg tattttgttc taaatatccg atttcacaat ctttagaagt gtaaatatct
     6541 ccactatcat atccataaat tcctgaaatt attttaaata aagtagtttt tccagtacca
     6601 ttaacaccga ttataccaat tttatcgcct tcatttactg taaaactaat attttctaat
     6661 atagaatcta taccaaaact tttatttaag ttattacatg acaaaacaat cataaatttc
     6721 tacctccttg aactatctaa aattataatg ttttgatata atataatcaa ggttatatgt
     6781 attagttttg aaaaagaaat attttattgt gaatcaaaca aaaaagagga cacttttagg
     6841 aggtgaagat gttgggaaat aaaaatatat caatggcagt tataagaagg ctcccaaaat
     6901 atcatagata tcttggagac ttattagata gggatataca aagaatatct tctaaagaat
     6961 tgagtgatat aatagggttt accgcttctc aaataagaca agatttaaac aactttggtg
     7021 gatttggaca acaaggatat ggttataatg tagaagctct tcatactgag ataggtaaaa
     7081 ttcttgggtt ggatcgacca tacaacgcag ttcttgtagg agcaggtaac ttaggacaag
     7141 ctatagccaa ttatgcagga tttagaaaag ctggattcga gataaaagct ttatttgatg
     7201 caaatcctag aatgataggt ttaaagataa gagagtttga agtattagat tcagatactt
     7261 tagaagactt tataaaaaac aataatatag atattgctgt attatgtata cctaaaaatg
     7321 gagcacaaga agttattaat agagttgtaa aagctggaat caaaggtgta tggaattttg
     7381 cacctttaga tttagaagtt ccgaaaggtg ttatagttga aaatgtaaac ttaacagaaa
     7441 gtttatttac cttatcgtat ttaatgaaag aaggaaagta gacaacttaa acatattaat
     7501 aatgagcagt tgtgaagagt attgagaaag tcagaagatt taatattaaa tcctctggct
     7561 ttttgaaata ttaggataaa aatttaaata atgtatggaa ataaacaata ttaaggagag
     7621 tggtttttgt ggaggaatta tggaaaaagg caattgaatt tcatggtcat gaatgtccag
     7681 ggttagctat aggatttaaa gcagcacttg cagcaagaga atatttagga tgtaaacagt
     7741 caggagatga ggaaattgta tgtatttctg aaaatgatgc ttgtggaata gatggtatac
     7801 aagtcgtgct aagttgcaca atgggtaaag gaaatttact acttaggatg actgggaaat
     7861 cagtatatca tttctttaat agggaaaatg gaaaatctat aagaatatat gtaaagtcaa
     7921 agccatttca aatggataga gaaggataca aaaagtactt attagaaagt gattttgaag
     7981 aaatatttga gattacagat actaagttaa gacttcctga aaaagcaaga atatttaaat
     8041 ctcttaaatg tgaagtgtgt ggtgaatttg catctgagca atatataagg attgaagaag
     8101 gaaagaaagt atgtcttgat tgctttaata aatatgaaag attttatgag gagtattaaa
     8161 attataaatt agattaaagt taaaagtatt agatatgtgc taacagtatt ttaagatgaa
     8221 ttaggtagca acataataaa gtatataaat aatattaatt gaatcataca taattaaaaa
     8281 gaggctgatt agtatttata aaataaaatc agtctcttta tgaataacga attattattc
     8341 tggttgaggt gcatctacag gacaagctcc tgcacaagaa ccacagtcta tacaagaacc
     8401 agcatctata acatatgcgt cgtctccagc tgatatacag ctaacagggc attcagcctc
     8461 acaagcacca cagcttatac aagcgtcagt aattttatat gccattttga aatcctccct
     8521 tttatatgta ttgtaattca ataacattat aacaaatatt attgaatata acaattaaat
     8581 taattctaca acaaaaactt tattatgata ttcttgtttt aaagttttac aataaagttt
     8641 aatctgctta tatttttatt tattttaaag aagtttattt taaaaataaa attaaattta
     8701 aaaagttatg tattgattat atttaaaaat gttatactct aaaaaagtat attttattta
     8761 aaaatttatt aatacaaagg agatttacta tgaaaaataa attagtttac atatgtatta
     8821 tgagtctatt gctatttgta gtaggatgtt ctaataatcc aaataaggaa agtaccatta
     8881 aaaaagactc taagaataac caaagccaag ttaaagaaga aaaaagagaa cctgtgaaaa
     8941 aagatataac tataagtttc gctggtgatg taactatggg gaactataaa ggttcatcat
     9001 attatggcag ttttgaccat gaatttaaaa gacaaggaga agattacaat tattttctta
     9061 aaaatgtaaa acctgtattt gaaaaagatg acttaacagt ggttaattta gaaggtccac
     9121 ttactacagc gtcaaatgca aaagaaaaaa aatttgcatt taaaggaaag cctggatatg
     9181 taaatatttt aaaatctgga agtgtagagg cagtaagtat tgcaaataat cattcagaag
     9241 attattttga agaaggaatg aaagatacac agtttatatt ggacgagaat aaaataaatt
     9301 attttggatt agggaaagat gctgttgtag atgtcaaagg aattaaagtg ggtctattag
     9361 ggtataatgg attatcaact gaatataatg aaaaaaattt aaaacaaatg gaagatgata
     9421 ttaagtcctt aaaaaagaaa tcggatgttg ttgtagtgta ctttcattgg gggatagaat
     9481 tagattatta tccagataaa aaacaaaaag attttgctca ttatgctata gacaagggag
     9541 cagatttagt tatggggtct cacccacatg tgattcaagg aatagaaaag tataaaaata
     9601 agtatatagc ttatagtcta gggaattttt gctttggagg aaataagaat ccttctgata
     9661 cagatagtta tatatatcaa caaaccttta ctttttcaga taataaatta acttcaatta
     9721 aagagccaaa tataatacca acttcaataa catctagtaa ctccagaaat aattatcaac
     9781 caaaaatatt ggatggaagt gagaaggata gagttttaaa taaacttgaa aaaataagca
     9841 aagatttaaa taaataaaat gtaaaaaaaa ataaatatat atatttattt taataattta
     9901 tattttgttt agggtatggt aaatcgtggt attaaataag tagattttaa tagttaaatt
     9961 atagtcaatt ataataaagc tatttaatct acttttttaa tataaaaata taagggggaa
    10021 tttgtaatgg cttgtaacac ttgtaaaatg tgcgaaagtg cagacaagaa attagaaagt
    10081 tttgtagctt caaaggatgt tgaaacagca tttcatagaa cagaagatca gaaagttaag
    10141 tgtggttttg gacttcaggg ggtttgttgt agactctgtt caaatggacc atgtagagta
    10201 actcctaaat caccaagagg tatatgtgga gcagatgcag acactatagt agcaagaaac
    10261 tttttaagag cagtagcatc aggagcagct tgttatttac atgtggtaga aaatacagct
    10321 aagaatttaa aaaatgttgg aataactaaa ggtgttgtta aaggagaaaa aacattaaat
    10381 caattagcag agatgtttgg aatagaaagt gatgaaaagt atgataaatg tataaaggtt
    10441 gcagacaaag ttatcaacga tttgtataaa tcaagagatg ataaaatgga attagtagaa
    10501 aaaatagctt atgcacctag agtgaaaaag tggaaagagt taggtataat gcctggtggt
    10561 gcaaagtcag aagtttttga tgcaatagta aaatcatcaa caaatttaaa tagtgaccca
    10621 gtagacatgc tagttaattg tttgaactta ggtatatcaa caggattata tggtcttaca
    10681 ctaactaatc ttttaaatga tgttatgttg ggagaaccag ttataagaat ggctcctgta
    10741 ggatttaatg taatagaccc agactacata aatataatga taacaggcca tcaacattca
    10801 acatttgcaa atttccaaga tagattaaaa gatgaagatg ttataaaatt agctcaatct
    10861 gttggtgcaa aaggctttaa gctagtagga tgtacatgtg ttggtcaaga tttacaatta
    10921 agaggagaac attatcaaga agtatttgct ggacatgcag gtaacaattt tacaagtgag
    10981 gctgttttat caactggagc aatagatata gttctttcag aatttaactg tactatacca
    11041 gggcttgaac caatagcaga taaatataaa gtaaaaatgg tttgtttaga tgatgttgct
    11101 aaaaaagcga atgcagaata cataggttta gacagaagta aattagatga acttagtaac
    11161 actttaatag aaaaagcatt ggagtcttat aaggagagaa gaggaagtat tgaaatagat
    11221 ataccaaaag accatggatt tgaacaatct ttaactggtg taagtgaaaa gaacctgaaa
    11281 gcgtttttag gagattcatg gaaacctctt attaatttaa ttgcagaagg aaaaattaaa
    11341 ggtgttgcag ctgttgtagg atgttcaaat atgacagcag gaggacatga tgtaaatact
    11401 gttgaactta ctaaagaatt gattaaaaaa gatataatag tactatctgc aggatgttct
    11461 actggaggat tagaaaatgt tggtcttatg tcacctggag cagaagaatt agcaggagaa
    11521 aacttaaaag aagtatgtaa gactttagga ataccaccag tattaaactt tggaccatgt
    11581 ctagctatag gaagactaga aatagtagca acagaattag cagctgaact tggaattgac
    11641 ctaccacaat taccattagt gctttcagct ccacaatggt tggaagaaca agcattagct
    11701 gatggagcgt ttggtcttgc ccttggctta cctcttcatt tagctttacc tccatttata
    11761 acaggaggaa aattggtgac agaagtatta acagaaaaat taaaagactt aacaggagga
    11821 catgttatag tgaatccaga ccctaaatct tcagctaatc aattagaaga aataataata
    11881 gatagaagaa aaaatttagg gttaaaggat gtggaatgca atgcatagaa tttttataaa
    11941 taaagattta tgtacaggat gtaagagctg tgtactagca tgtatgctaa aacacaataa
    12001 agattatgac atgtacactc ttgatttaga aaatatagat aatgatagta gagggcatat
    12061 agaattagat agtaaacata ataatccagt tccaattctt tgtagacact gtgatgaacc
    12121 tgaatgtgta ttagcatgta tgagtggtgc aatgcataaa gatagtgaaa gtggaatcgt
    12181 ttcatatgat gaagagaaat gtggttcatg ttatatgtgt gtaatgtctt gtccatatgg
    12241 attattaaag ccagatgata gaagcaagca gaacatactt aaatgtgatt tatgtaaaga
    12301 tgaggagtat cctagatgtg tagctaattg cccaagtgga gcaattgaat tacagaagga
    12361 ggaaaatgat gaattatgta gtgttagggg ctagtgcagc tggtataaat gcagtaaaaa
    12421 cattaagaga attagataaa gattcaaata tagtagtaat ttctaaagat gaaaacgtat
    12481 attcaagatg tatgttgcat cacgttatat ctgaacacag aattttaaaa caaataaatt
    12541 ttgttgatga agattttatg gagcaaaata atgtaaaatg gattgcaggt aagactgtta
    12601 aaggtataga cataaataaa aaggtagttc agacagaaga cattactgta aattatgata
    12661 aacttttaat agctacaggt gcatcttctg ctattcctcc tataaaaaat ttaagagatg
    12721 gaaattttgt atattctgtg agaaacatag atgatattta taaaataaaa gaaaaagcac
    12781 aaaattctaa aaatgtagtt ataataggtg ctggtttagt tggtatagat gcattagtgg
    12841 gattatttaa atatgagcag ttaaatattt ctgtagcttt tatggaaaag tacattttag
    12901 atagacaatt ggatgaatat acagcatcag tgtatgaaaa taagttcaaa gaaaaaggtg
    12961 taaaatttta cccaagtgca tcaatacaag aaattgtatt ggataacagt aaaaatgtta
    13021 caggtgttgc attttcaaat ggtgaagttt tagatgcaga catggtaatt gttgctacag
    13081 gggttaaacc aaatgctgac tttttagatg gaacaggaat agaatatgat agaggtataa
    13141 taatagatga tatgtgccaa acaacacaaa aagacatata tgcagcagga gatgttgtag
    13201 gtaaaaatgc tatttggcct ctggcagtaa agcaaggaat tgtagctgcc tataatatgg
    13261 ttggaaaaga taagaagata gaagatgaat ttgcttttaa aaacagtatg aatttcatgg
    13321 atataccaac tatttctata ggaatgaata cacctgtaga tgatagctat aaagtgctaa
    13381 ctagacatgg aattaatgat tataaaaagt ttgtttttaa agataatgta atctatggag
    13441 cagttataca aggagatatt tcatatgtag gtgtattaac ttatttaata aaaaataaag
    13501 ttgaaatata tgatttagaa aataggatat ttgatatatg ttatgcagac ttttttaata
    13561 taaaagaaaa tggagaattt tgttatagtg tatagtatat acgtggagta aggtttataa
    13621 atacagtaaa tcaataaata gagttattct aaaataagaa aaatgacaaa tcttatttag
    13681 aaatagctct attttttgta attaaattta aattatattc aatttgtaaa atattatttt
    13741 gaacaattat acatatactc tctagttaga ggtaaatcat tttttatacc tttagtgaaa
    13801 agtatttgat gtatatctat aataccagtg ttgaaagcag cagcacaaga acaaaggtat
    13861 aaatcccaca ttcttataaa cttatcatca aacatttctt taatctttgg aagattctca
    13921 gagaaatttt tttgccagta tagtaaggtt tttacataat gtaatcttag actttctacg
    13981 tctatagtat gatatttgta atcactacaa atatgtatca tttctcttag gctaggaata
    14041 actcctcctg ggaaaatata tttttttatc caagggtctc cataagattc ctgcaatcca
    14101 cttatatagt gaagtaaaaa caatccacca tcttttaaaa cagcatttac gtttttaatg
    14161 aaaagttcat agttttctct accaacatgt tcaagcatcc caacgcttac aattctatca
    14221 aacataaggt ctgatttttt taattctcta tagtccataa gtcttacttc aagatagtct
    14281 tgaaggttct cttctttaat tcttgcgtta aattccttaa attgctcttc gctaagtgta
    14341 attccaacgc cgtgaatttt atattttttt gcagcttcta ttagtaaaaa tccccaacca
    14401 caaccaatat cgagaaggct cataccttcc tctaaattta gtttagttaa tatgtggtga
    14461 actttattga cttgagcatc ataaagagta tcattatcat ttctaaagta tgcacaagaa
    14521 taactaagtg tcttatctaa ccataagcta taaaaatcgt tgccaatgtc atagtgggca
    14581 tgtatttctt tcttttgatg tctcattgaa tttgatgagt gcaatagatg ttttacagct
    14641 gaattatcaa tattgaattt gtctatttgt tttaaaagta gatttaaagt taagaataaa
    14701 tctccctcaa cttcaatgtc tctatttatg taggcttctc caagtgctaa agatgtactt
    14761 tttaatagtt cagacttgct aatatcatta tttactataa ctttgaactg aggggagccc
    14821 tctccaatta gaaattcttc ctcatcttta agcctaacta gaaatggtgt ttcaccaaat
    14881 ttctctaaat agtcttttaa aaaatttttt gtaatattca tattaccagc tccttctaat
    14941 gtaaatccag taagtttccc tgcttaaaat atattatagt ccttttttta aaaaagtaaa
    15001 attttttcaa taaatttttt atttaaagga aatttatttt ttataaagaa atttcatgtt
    15061 gtagtatatt ttttgcttat aaattttggt actggatata atattaaact gttaatgata
    15121 taataaattt aatctataaa gtaaataaaa ttttgtaata aatagttaaa gctattttat
    15181 aaggagatat atgatggaag tatatgcttt ggttgggtca agtggcacag ggaagagctt
    15241 taaggcttta gagttcgcat atgaaaatga tatagaatat ataattgatg atgggatttt
    15301 aatatacaaa aacaaggttt tagctgggat atcggcaaaa caagcaaata ctactataga
    15361 agcagttaaa agggctattt tttataatct ggagcataga caagaagtta gagaagcaat
    15421 taaaaaagaa aatataagaa aaattcttat tattgggaca tcaaaaaaaa tggttaatca
    15481 aattgtagag agattgtcaa ttggaagggt ttgcaagttt ataaatatac aagatataag
    15541 tactaaatat gaaatagaaa tagcaaaaca agctagaaaa gaaggaaatc atgtaattcc
    15601 tgttccagca gttgagataa aaagtatggc aagtggtctt agtataaatt ctttgaagag
    15661 actttttaga aaaggaagta ataggaatat ggtagtttta gaaaaaacaa ttataagacc
    15721 aacttttagt tatataggaa agttttatat tagtgctgat gtaattaaac agattattga
    15781 atatgaagta tacaactttg gaagcattga tagaataaat aaaatcaata tagagaattg
    15841 caataatttt atgaatattt ttgtaagtat aaatatcaat gatttagaaa ctataaaaaa
    15901 aattgaagac atacaacaga caataaaaaa gtctattgag aaaatgactt tagttaatgt
    15961 acaaaaaata gatattaaca ttcataaaat taaaaaaata aacttcctcc aatgttaaaa
    16021 aataaactga gaaaatgata tactaatttg caataaagtg tgctataatt aaactgtaaa
    16081 tttaggaaat ggttttttct aaatggagta tacataatta taacgtattt ataagggggc
    16141 tttcaaatgt caggaaaaga tgtaaatgtc ttcgagatgg cgcaatctca agtaaaaaat
    16201 gcatgtgata aattaggtat ggaaccagca gtttatgaat tattaaaaga acctatgaga
    16261 gttatagagg tttcaattcc agtaaaaatg gatgatggtt ctataaaaac ttttaaagga
    16321 tttagatcac aacataatga tgcagtaggg ccaacaaaag gtggaataag attccatcaa
    16381 aatgtttcaa gagacgaagt aaaagcttta tctatatgga tgactttcaa gtgttctgta
    16441 acaggtatac catatggtgg aggtaaaggt ggaataatag tagatccatc aactttatct
    16501 caaggtgaat tagaaagatt aagtagagga tacatagatg gaatatataa attaataggt
    16561 gagaaagttg acgttcctgc accagatgta aatactaatg gtcaaataat gtcttggatg
    16621 gttgatgagt acaataaatt aactggacaa agttctatag gtgttataac tggtaagcca
    16681 gttgaatttg gtggttcttt aggaagaaca gctgctactg gatttggtgt tgctgttact
    16741 gcaagagaag ctgctgctaa attaggaata gatatgaaaa aagctaaaat agctgttcaa
    16801 ggtataggaa acgttggttc ttatacagtt cttaactgtg aaaaacttgg tggtactgtt
    16861 gtagctatgg ctgaatggtg taaatcagaa ggttcttatg ctatatacaa tgaaaatggt
    16921 ttagatggtc aagctatgtt agattatatg aaagaacatg gtaacttatt aaacttccca
    16981 ggagctaaga gaatatcttt agaagagttc tgggcttcag atgttgatat agtaatacca
    17041 gctgcattag aaaactctat aactaaagaa gttgctgaat ctataaaagc taaattagtt
    17101 tgtgaggctg ctaatggacc aactactcca gaggctgatg aagtatttgc tgaaagagga
    17161 atagttctta ctccagatat attaactaat gctggtggag ttacagtttc ttacttcgag
    17221 tgggtacaaa acttatatgg atactactgg tcagaagaag aagtagagca aaaagaagaa
    17281 atagctatgg ttaaagcatt tgaatctatt tggaaaatca aagaagagta caatgttaca
    17341 atgagagaag ctgcttacat gcattcaatt aagaaagttg ctgaagctat gaaattaaga
    17401 ggatggtact aagatttact cttaataaat aataggatat aattatttaa gatataaagg
    17461 ggctgtacat agtacagccc ttaaattatg tggcttacaa tgaagtaagc tatttttgtt
    17521 ttaacttata aaaaacttga taaattatgt aatttagaat gtatttttta ccaccacatg
    17581 attacaaatt agaagagtta gattagattt ttagacatga acttatgcat tttaaaagca
    17641 aagaccttaa tttaaagtat ctagtattat ttttaaaaat agtatattgg tttaatccat
    17701 ttatatatat aatggaaaaa tatatggatt tggattgtga actttattgt gatgagagag
    17761 ttttaaaaaa ttgtactgta caacaaaagc aagaatatgc tctaactatt gtaaattcaa
    17821 tgaaaagaag tacaaatttt tcaaataaat ttgtagctgg attaaataaa caatctgata
    17881 ttagaaaaag agtaataaac atgttcagtg aaaaacataa aaatggtatc ttaagggctt
    17941 tgattataag cttgttttct tctattacat tttttaaatt cgattcaact agtattattg
    18001 aagagaatga tttcttagag aggtcaagat atacgtttga aggaaggcaa gaagacatgt
    18061 ttgtaacaat agattttata tatgcagatg tacctataaa acttagaaaa gagcatgaag
    18121 aaaactgtag agtcaatgga atagtaccaa aagattcaga ttcaatagaa attattccta
    18181 gattttataa agattgtatg gatttaaaaa aataagttag acttaatatt gatgttgata
    18241 ttatgaattt tttatattta agcaattgaa aattttatta tagattcaat tttatatgtg
    18301 tttaagtatg aaatttatag atagttgaaa taattaaata tgattgatgc ataggaatca
    18361 ttactaagct ataattaatt ataagggagt ttaaataaat tatgagttac acaattataa
    18421 aatgataaaa aatagttatc tgttacaaaa aatgtatctt tttaattaat aaagataata
    18481 tgaaaactta aaaggcaaat aaacttaaac ataaaatact cttaaataag ataggagaaa
    18541 aatgtatgtc taactttatt ctaaatggga tttttagaac agttatagta agtagtctaa
    18601 gcattcttat gatattgatt ttcaagaaaa atgtatttaa aagatttagc aaaaaattta
    18661 atta
//
LOCUS       DAMJYX010000070        17747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJYX010000070 DAMJYX010000000
VERSION     DAMJYX010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.071"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            508..1368
                     /locus_tag="PC479_002513"
     CDS             508..1368
                     /locus_tag="PC479_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2805052.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1358..3229
                     /locus_tag="PC479_002514"
     CDS             1358..3229
                     /locus_tag="PC479_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2805053.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3249..4703
                     /locus_tag="PC479_002515"
     CDS             3249..4703
                     /locus_tag="PC479_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2805054.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5728..6936
                     /locus_tag="PC479_002516"
     CDS             5728..6936
                     /locus_tag="PC479_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805055.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6967..8346
                     /locus_tag="PC479_002517"
     CDS             6967..8346
                     /locus_tag="PC479_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805056.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8452..9642
                     /locus_tag="PC479_002518"
     CDS             8452..9642
                     /locus_tag="PC479_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805057.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9770..11098)
                     /locus_tag="PC479_002519"
     CDS             complement(9770..11098)
                     /locus_tag="PC479_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805058.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11716..12930)
                     /locus_tag="PC479_002520"
     CDS             complement(11716..12930)
                     /locus_tag="PC479_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2805059.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13356..14072
                     /locus_tag="PC479_002521"
     CDS             13356..14072
                     /locus_tag="PC479_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2805060.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14207..14833
                     /locus_tag="PC479_002522"
     CDS             14207..14833
                     /locus_tag="PC479_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2805061.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15033..16577)
                     /locus_tag="PC479_002523"
     CDS             complement(15033..16577)
                     /locus_tag="PC479_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2805062.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(16603..17244)
                     /locus_tag="PC479_002524"
     CDS             complement(16603..17244)
                     /locus_tag="PC479_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2805063.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            17514..17705
                     /locus_tag="PC479_002525"
                     /pseudo
     CDS             17514..17705
                     /locus_tag="PC479_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
BASE COUNT         6557 a         2085 c         2926 g         6179 t
ORIGIN      
        1 tattagtata tcttactagc atttaaatga aactaaattt gaagctttac ctattaatat
       61 atcttaatag tgattaaata aaactaaact taagacttta tatgttaata tatcttaaga
      121 atttatttgt taaaatacag ttcattatat ttaaaagcat tactatgata attcataata
      181 gtaaaataga taatggttca gacaatatta taaatactag tgacggtaaa atagtatcaa
      241 tttagtatat aaaccttgtc agataatcgc ataacagagc ttgtagttat agaataaaaa
      301 aatatctagg aaaccgttga cagatttata tggatagtat aacatataga atatgaaatt
      361 aaacaaaaaa gaattttaat tatggcgacg tagtcataat tttgagtatg ttactgatta
      421 gcaggcagaa ctcaagtata tatgtgaaaa atagagggat actctataca tatatgcttg
      481 agttcttttt attgaaggga ggaatatatg aaaataattc agattttaaa taataatgta
      541 gccttagtaa aaagaggtaa aaatgaaatt attgtatttg ttaaaggaat tggctttaaa
      601 aaaagagtgg gacaagtaat aaatgaaaat gaaatagaga gaacatatat actagattca
      661 tacgatatgc tagaacactt tagttatcta ctacaaaact cagactccaa taatatagtt
      721 cttattaatg aaataattga atatggtgag aaaactttaa atataaaggc aagtgattat
      781 ctaagcttaa ccttactaga ccatttagag tttttactta aaagatgtaa aaagaatcaa
      841 tttataaaaa gtcctttggt atggaatgtt aaaagatttt atccaaagca ttttgaaatt
      901 ggaatgcatg cactaaaatt aattggtgat agtaagggaa ttaatcttcc tgatgatgag
      961 gcagtatcaa tagcacttca ttttgtaaat atggaggtaa ataaagagtc tcatgatagt
     1021 acaattgtag agttgagaac tttagcagat atagtttcta taataaaata tcattttaat
     1081 gtagagctag atgaaacttc aacaaattat atgagattta ctactcatct acagtacttt
     1141 atccaaagaa ttatgtcaga ggaagcttca atagatgaag ataattctat agatttatat
     1201 aaacaagtaa gtcaacttta tccaaagtca tttggaagtg ttcaaaagat aagaaaatat
     1261 ataaaatcac aatttaatgt cgatatatct ataagtgaag aaacttattt aatgttgcat
     1321 ataaataggg tgactcaaag actggaggca aaatcagatg aaatataaag atttaaatga
     1381 aaatataata aaacttatag ggggaaaaga aaatataaag tcggtagcgc attgtgttac
     1441 tcgtttaaga tttactttaa atgataggag tatcgctcaa acagaagaaa ttaaaaaatt
     1501 aaaaggagta attgatgtag tttctaatga tgttgcttat caaattataa taggtactca
     1561 tgttgtagat gtatataacg agtttatgtc tatgataggt ttaagttcaa gtgaatcaag
     1621 tataaaaagt gaaagaaaaa agggaattag aggtatcaaa ggtatattta attccatctt
     1681 tgtggtagtc tcagagacta tgacttcagt aattgaagta ctacttgcag ctggtatact
     1741 tgcaagtata ttagcacttt taaatatgtc tggcatactt cagagtgaca gtcctacata
     1801 tcaaatatta gatacattaa gaagtgctac atttcacttc ttaccagtat taattgcaat
     1861 agcatcagca aaaagactta atgtaaatcc atatatagca gtagtattag cagttacgtt
     1921 attatcttca tcaatagatg gtgtagaagg attaagttta tttggtataa atttacagtc
     1981 tacaacttat gcaaacagtt ttattccaat acttttagga gtatgggcaa tgggaattat
     2041 aattgaaggt ttgaaaaaaa tattacctaa agcactacac tattttttag ttccagtatt
     2101 atctttaata ataactctat cagttacatt aactgtattt ggacctattg gtatgtggat
     2161 tggtgatgga ttaaactatg catgtatgtt tttaattgat acattgggta attggagtgt
     2221 tgttgcactg tatgcagctt tacaaccact cttaatagtc tttggtgcag caaactttac
     2281 tatgcctata tctttaaatt ctgtgacaac attggggtat gacccaattt ttatgggcgc
     2341 tgcaacaata tctgatattg cagtttgtgg tgcaatgttt ggatatttct taaaaactag
     2401 gaataaagaa caaaaacaat tatttggtac agtaagtttt agtgcactta tgggagttac
     2461 agaaccttca gtgtttggtg tgtttatgaa atatagaaga ccatttgtag cagtaatgat
     2521 aggtggaggt ataggtggtt taatagcagg tttagctcaa gttaaaacat atggaatggt
     2581 ttggggatta gctgcattac caacgtatct tgttggagga gaggtatcta actttacatt
     2641 tatgataata agtgtagtag tatcatttgt agcagcaaca gcagcttcct atttactagg
     2701 aataccttca gatgaaaaag aagaagttaa gggtgataat gaaaaagaat tagaactaat
     2761 aatcaacgag aacaattcaa gaactattag tattggaaat gttgcaaaag gtcaggtaat
     2821 tgcacttggg gaagttaatg acaaagcatt ttcctctgga gcattaggta aaggggttgg
     2881 agttatacct agtgaatcaa aatcaactgt tatatctcct gtagatggaa ctataactat
     2941 agtatttcca actaaacatg catatggtat agtgactgat gaaggattag aaatattaat
     3001 tcatattggg atagatactg taaacttaga tggaaaattc tttgaaagtc ttgtaacaca
     3061 aaatcaaagg gttaaaaaag gagatgcttt agcagtattt gaatctgata aaattataga
     3121 ggaaggcttt gattctacta taataactgt agttacaaat actagcgatt atttagatgt
     3181 aatctcatca aatgatgaat cagaagaatt attaactgta atagtataaa ttttaggagg
     3241 gaaaattcat gaatattaaa gatacatttt tctggggagg aagtattgca gcccatcaat
     3301 gtgaaggttc atgggatagt gacaacaaag gacctgcaat tatggatttt gtaactaagg
     3361 gctcttatga aatgccaaga gtaattactg ataaaataga agaaaaattg gattatccat
     3421 ctcataatgg aatagatttt tataatagat ataaagaaga tatagcttta tttaaagaaa
     3481 tgggatttag tgcattaaga atatctatag attggtcaag aatttttcca aatggagatg
     3541 atgaaaatcc taatgaatta gggattaaat actatgaagg tctgatagat acattaatag
     3601 aaaataatat agaacctatt gtaacactat atcattttga attacctatg aatttagtac
     3661 ataaatatgg ttcatggaat aacagaaaac ttatagattt gtatttaaag tacagtgaga
     3721 ctgtaataag aagatttgat gataaggtaa agtactgggt tacatttaat gagatgaacc
     3781 atattgaccc tcaaacagag cactcggata tttttactta tatgatagca ggtattaagt
     3841 atagtgaaat agaaaataag gttcaaacat tggcaaatgt agggtataat atgactttgg
     3901 cgggtgtaaa ggttgcaaag ttggctagag aaatcaatcc caacaataaa gttggatgtg
     3961 tatttggact aaatccaatt tatccataca attgtaatcc tagtaatgtg ttaaatgcat
     4021 ttttagataa tgaccgtgat tattatcaaa ttgatgcaat gtgcaatggg aagtttccac
     4081 aatataaatt aaaagaatat gaaaaaaata atataactat tgaagtgact gatgaagaca
     4141 aaaaagcatt taaagaaggt aagttagatt ttataggact taattattat ttttcaagtg
     4201 tatctactcc aaaagaaaat ttatcaggtg ataaatcttt atttggtggg attcaaaatc
     4261 catatttgga acaaagtaag tgggggtggg caatagaccc tataggttta agatttacct
     4321 tgaactatat ttacagaaag tatcaattac ctatcttaat tacagaaaat ggtttagggg
     4381 catatgataa aatcgaagaa gatggaacta taaatgatga ttatagaatt gaatatttag
     4441 gtaagcatct atctcaaatg aagtctgcaa ttgaagaaga taatgttgaa tgttttggat
     4501 atttgatgtg gggaccaatt gatttagtca gtgcaactac aggtcagatg agtaagagat
     4561 atggatttat ctatgttgac ttagatgatg aaggcaaagg aagttttaag agatataaga
     4621 aaaaatcatt tgattggtac aaagaagtaa taaaatctaa tggagaagtt ttagattttt
     4681 ctacggatat agtagtaggg taggaataag ataaatagaa gttaggaact aatgttaatt
     4741 agtgtctagt aaagcattta aaatagagta tagggtcttt aaagtaaagt gtgtattctt
     4801 cttaaaagta atggcaacta aaattactat aaaaaggaga atacacattt tttatgttta
     4861 tacttaagta aatatttaga catattcata gtataattta ctcattatat aaaaatagta
     4921 tgatttactc actatataac tttataaatt ttctggaaaa atgtcagaat atattgacaa
     4981 agaaaagatt taaaattata atctatatta agaatagttt gacaatataa aaacaagtat
     5041 ttttataaat agtgaaatta tttttattgt caatttaaga ataatttagg actactataa
     5101 agataaaggt atagatgtca ttatatgata aattatactt gtatagaaag aattttaaag
     5161 tatggcttat cattgattta atataataaa taatttttgt ttctagtaat agttcctagg
     5221 aataagatat ctaaattcgt ttagaaatag tctaatttac aaaggagggg atgcactaat
     5281 agataaaatt tctatataaa gaagacttaa agtgaagtat attctgataa tattggttta
     5341 atgggtatat tattatttcg ttttagatat tttaagaata atttaagacg tttataaaaa
     5401 tgtattctat atctaatatt taaataaata taagtcttcc aacatgtata ctaatataga
     5461 gagtagttga agtacaattc taactcgtaa aattatgtag aaagtaaaaa tcaaaaagaa
     5521 atctagtaag ttgataacta tagttaatat ttagagagtt aactctagtt atatttattt
     5581 agatatttta ggaataattt aggacgtata taatatcatc aaaaaatatc aagctatacc
     5641 atatgtagtt aagaataact gcataatata cattttataa ttattgtttt attgataaaa
     5701 aattattata tttaggagga aacaacaatg gaaaattttt ggaatccact tacttgcttt
     5761 actgtatgtg taatgattta tgcttttagt gagtttttat ctaagaaaac aaaaggtgca
     5821 atttcaacat tgttatttgt atgtattata tttttactag gattttggac aaatatttta
     5881 ccaaaagaca ttacaggagc atcagggata ttagctgcaa tgagcaactt tggtatagct
     5941 ttattaataa ctaacttagg aacacttata aatcttgaag acttatgtca tgaatggaaa
     6001 acggttgtaa ttgcattagt aggtattgta ggaataggag ttggctgttt aacagtgggt
     6061 agtatgttat ttggcagaga gtatgcttta atagcagcac ctccaatagc aggttctact
     6121 gttgctggga ttatcgtaac aactgttgca gaagctgcaa atagacctga acttgctgca
     6181 tttgtaattc tagtattatc atttcaaaag ttctttggta ttccgataac tacatattgt
     6241 ataaataaag aattaaaatt taaaagagat aacggggatt ttttgaaaga tgagtcaaaa
     6301 agtaatttta aacttccaag tatgagaatt tttaaagaag atttaggtaa aaaatcatct
     6361 actctagtgt atcttgcaaa gctttcattt gttgctatga ttggtaattt tgtaggattg
     6421 gctactttaa taccaggatt tacccctgca aactattatt taaatcctaa tatagcatgt
     6481 ttgttagtag ggttgatttt tacacgaata ggttttttag aaaaaggaat tttagataaa
     6541 tctcaatcta atggaattat aatgtttggt tgtatgttga tgttacctgg aggacttgct
     6601 aaggtaactc caacagcatt gtttgggatg attgttccaa ctataggaat acttgttgta
     6661 ggttctgtat ttataatatt atttactgca attgtaggaa aactattggg atattctcca
     6721 agaatgtcag ctgcaattgg tgtaacttgt atgctagctt atcctgcaac acagataatt
     6781 tccaacgaag gtattgatgc tatggaagga actgaagaag aaaaacaaag ggcaacagat
     6841 tatttattac caaagatgat tataggagga tttgttactg taaccattgc atcagtggca
     6901 tttgctagta taataggtcc aattatattt ggttgatagt aaatatactt ataggaggaa
     6961 taggaaatga taggaagtat atctataaat gagatagata aaaaaagatt caaacttgat
     7021 gatttaagta agaaaatatg ggaaaatcca gaaaaagcat ttaaagaatt taaagcatgt
     7081 gaaaatacag caaactttct aagaagtgaa ggatttgatg tggaagtagg tgtaggaggt
     7141 ttagccacgg ctattagagc tagttttggt tcaggtaaac cagttatagg ttttatggga
     7201 gaatttgatg cattacctgg tttaaatcaa aaagtttcaa caaagcaaga agcatttgaa
     7261 ttgggtgcat atggtcatgg atgtgggcat aatttattat gtacagcaca tgtaggggca
     7321 gtagtagggc ttaaaagaga gatgatagaa aataacttaa gtgggactat agtattttat
     7381 gcatgtccag gagaagaaca attaacaggt aaaggattta tggctagagg tggagcgttt
     7441 gaagggttag acttagctat aaattttcat ccaaataaaa ttagtgaagc tacaattggt
     7501 acttcaacag ctgttaactc tgtaaagttt cactttaagg gaaagactgc acatgcaggg
     7561 tcagaccctc aaaatggaag aagtgcacta gatgctgtgg aacttactaa tgtgggagct
     7621 aattatttga gagaacatgt tacttctgat gtaaggattc attatacaat tacagatgga
     7681 ggagtagcac ccaacatagt tcctgataaa gcatcagtat ggtattatac aagagcttta
     7741 agtagagaag ctgtagaaag tacatatgaa agacttgtaa aggtagcaaa aggagctgca
     7801 atgatgactg aaactgaagt tgaagtagag tttttaggag ggtgttataa tactttaaac
     7861 aatcatgttt tagcaaatct tgtatcagaa tgtatgaatg agattaaaca agagccttgg
     7921 actcgagagg aattggattt tgcagctgaa ttagatagac aaagcccaca acaatataag
     7981 gctatgataa gtaaatataa tttgccagaa ggaactcatc tatattatgg tggaggaaat
     8041 gttacaaact ttaatagtta tggctctact gatattggag atgtaatgca tatagttccg
     8101 acagcatact tttttacagg gtgtacgaat ctaggagcac caggtcatag ctggcagttt
     8161 acatcttgtg caggaagttc tataggagaa aaaggtatga tatatgcatc aaagattatg
     8221 gctatgtttg gagctaaaat attaaataat ccagagatag ctaaaaaggc aaaagaagaa
     8281 tttgataaat ctatgaatgg taaaacatat aaatgtccta ttccagatga tattccaact
     8341 ccttaaaaag acatgaaatt taatatataa aattataatt tttatatcag catcatttca
     8401 ttactaaaag aaagtgagat tttgctgact atctgattag gaggaaggaa tatgaaaaat
     8461 ttttttaaag aagcgcagtc aatagaaggg gaaattatta gatggagaag gtatttacat
     8521 gaaaatcccg aaattggttg caatctaccg aaaacagtaa cctttgttac cagtacttta
     8581 gaaaaaatgg gatattctcc gaaattgacg tctaataatg gaatagtagc tgttttagac
     8641 agtgaaaatg gtggaaagac cttattatta agggcagata tggatgcact tcctattagg
     8701 gaggagacgg atttaccttt taaaagtaag aataaagatg catctcatag ttgtggacac
     8761 gataatcaca cagcaatgct cttaggagca gctaaaatac ttatggataa taagcactta
     8821 cttaaaggta gagtaaagtt tgtatttgaa ccagatgaag agactaccag aggtgcaaaa
     8881 tcaatgattg atgatggtat attagaaaat cctcaggtcg atgcagctat ggcatttcac
     8941 agtatggtgg gtaaatttct taacacaggt caagtagctt attcacgtgg tccagctatg
     9001 gcatctgcgg atatatttga aataacagta gaaggaaaag caactcatgg agcaagccct
     9061 gaatttggag tggacccaat aaacataatg tcgcatatac aaatagcatt aaacactata
     9121 ctttcaagag aaaaacctca gaaagagcct gtagttttaa ctattggtat gtttaatgca
     9181 ggaaacgtac caaatgctat accaaataaa gcatctatgt caggaacttt aagaacattt
     9241 gaccaatttg ttagagaaaa attaaaaaat agaattattg aaatatctac aggaatagct
     9301 aaatcatttg gaggaaatgc taaagtggag tttaaaatgg ggactcctgc agttataaat
     9361 gatttggatg taggagatga gattgtatct tatgttaaag aagttatagg aaatgaaaat
     9421 gtaatagact ttccatctgc aatgggttca gaagattttt cagaggtttt actaagggtt
     9481 ccaggagtat ttttatggat tggaatggga agtattagtc agggatataa ttttggtatg
     9541 cataatccta aagcaatttt taatgaagag ggattagttt atggttcagc tttatttgct
     9601 cattgtgcag taaaatggtt agaaaataat tttagtagat aataacataa aatattatta
     9661 aatatcataa aaggtatatc ttaaaaagat gcaaagatat accttttaga aagtcatata
     9721 taattttact taatattaga tgataaaata tttttatgca ttttggattt taatatttat
     9781 tttctgatat aaataatttg tatacccttt caggtctgcc ttttgttgag ttttgttttt
     9841 catataatat agtagcgttt ccagacttta agagactact tagaaatcta ttagcagttc
     9901 taactgttac atttaaacat gaagataatt ccttaggagt aacttcatct ctttgtagaa
     9961 tttcagtaac agatataatc ttttgaatag taagagtaga caatttagaa cttttggcaa
    10021 ttgtatttat atgtggagtt actttatttg aaacagtaag taacttatca cacagtaaag
    10081 gtcctattgt ctcataattt tcgttaacta agaaactgta atttaaagga tttattttag
    10141 attccttatt agcacttatg gcactagaca tagcttgctt tatatcatta ccaattccat
    10201 atccaataca tactttaata tttgtactat tttcaataga ttcttttata taacatgcag
    10261 taaaattttt agttaatgat ttaatcgttt cacatttagt gaaaatttta aaagaattac
    10321 tatttttttc aattataaaa tcggttaatt tatctttttt aatactattt aatattttat
    10381 aaacttggtc tagagtagag tcatcataat ttgtatcttt aactgttata tatattacac
    10441 ttggttgatt tcccttcatt ctatgtaaat gaatatcttt tattaagtta ttaaaagtat
    10501 tttttaataa atctatatca gggtatatga aatgaaagtt tactcctgca ttttgtaaca
    10561 taggaacaat actactaaaa cgagtagtag aaaaatcgat tttcccttca ttccatagat
    10621 taatatgttt ttttgcaatc tttttttcaa gttttgctat gtcagataaa ctcattttag
    10681 atgcattttc atcaatacta tccattaagt ctccaaatgt gccatttgat aaataatatg
    10741 atatatcttt tggattatct gcccagtctg ttaagtcaaa gtatgctctg gaaaaatcta
    10801 atgtattata tttaaagata agctttagaa acatatcata gtaactttgt aagtcagtac
    10861 caaaatcaac tagaggtttt ttaatagttc cagccttttt tgtaatagct tttttgggga
    10921 tggggccact aactgcaaat ccatcaaacc tatcttctat ttctaaatat aaatctccaa
    10981 catgagaaaa atctctatag atatatattt caatttctgc attaatattt aactttttta
    11041 tagaattatc tacaaactct tttaaaaatt cggttgttat gactgctatt ttcatcatag
    11101 attacttcct tttcaattat tataattatt ttatattaaa tattacatta atctattgtt
    11161 ctagttaaaa tcgtatttca attaaattca attaaaataa ataactcttt ataaaaagca
    11221 attatttagg tagaggaata tttgctaact ttaatagtct ttgttaactt taattttatt
    11281 taagttatta tagtcttact aattttataa ttgtagattt aactacttta tgttctttta
    11341 ttgtttatgt agttgattta gaagttattt taaccattta ttttaattga agttgtttga
    11401 cttttaaatc ctttgtgtaa tctttgtttt tctagccatg ataaaagtat ttctatttgt
    11461 atcactatat ataataatat catgacaaaa tatttattgt ctaactaatt ataatctatc
    11521 caaaatgaaa atgggtgtga gatattatta caacaaattg tattagtatt aaaaagatta
    11581 aaaaattaaa aaactaaaca gttaaataat taaacgatta agtgactgtc atataacaat
    11641 ttataatttt atttaacagc cacttaggta tttaagagcg atagaattga gtgtatatca
    11701 aattataata atttcctagt aaataattga aaaataaggt agtccatttt tatatttgat
    11761 atctggttta ccttgaataa gtggatatag ataatttagg gcttcatcag ttatataatt
    11821 acattcttca tttatccact ctttaggaaa atattttgtc ttgttagcta ttttagaaat
    11881 atcagtatga ctaacttcat acttataagg attattgcat gttctattaa tgatagccat
    11941 aactcctgta tatccttttt tagatagttc tatcccatat gcaccaactt cagctgcttg
    12001 ttttaaatca gtatctgaag ctaaatgcat agcacatctt tgtattacac caagttctaa
    12061 ggtttttact cttgttgtaa ttgatgatga tactataaga ttctttagat agttgcttac
    12121 tccacctaat tgggaatgac caaatttatc atgagcagaa gttttttgtt cgcatataaa
    12181 gttgccgtat ttatccctta taccttctga tgcaactata taagccttat ttttttcttt
    12241 aaatattttt ctaacatctt ctataaactt atcttcatag aaatcaattt ctggaaggta
    12301 tattaaatca acaagtggag tatcattaag tctagctagt gaagaagaag ctgctaacca
    12361 tccagtatct ctacccatag tttctactat aaatatacca ttatttttgt atatagaaaa
    12421 atctaagtat gtttccatta ttagtgtaga aataagcttt gcagcagaac caaatccagg
    12481 acagttgtca gttcctaata aatcattatc tattgtttta ggaataccta taaattttat
    12541 tttacaagta gtgtttttgc agtattgaga aaatttcatc actgtgtcca tagaatcatt
    12601 tccgcctata taaaataatg tatctatttt atattcatct agtatattta taactctatc
    12661 ataatcataa gaatttattt tataatcagc cagtttgtat ctacaagaac caagaccaga
    12721 cgaaggtgtt tgctttaaat ttaatacatt atctttgttt aaggagtctg aatctattat
    12781 atttttgttt aatatacctt caatcccatt gataccaaaa tatactttat caaagtattt
    12841 agtttctaaa tttttttcat aaataccaac tgcagaagca ttgataacag ctgtagggcc
    12901 tccagattgg gcaataatac agtttctcat tttacatctc cctttcatta atattgttat
    12961 gaatggtact ttttatcgag taaatttata tttatagtct gattatatat taaatgaact
    13021 agctatatca attgagttag tatagacaat aaatgccata agaatgtggt aaaaaactaa
    13081 ctttttatat acaattaatg tctataattt accacaatat aaaggtactt ttaaggagta
    13141 gtcaaagtgt tgtgaaaaaa aatatatcag tgtaatatta atttacaaag caaaataagt
    13201 tcacagttaa caaaagcaga ataagttcat aaaagaaata tatacagcaa taaaagtaat
    13261 gctaaataaa agtaatactg gattaaaaaa tattaaaaaa atattaaaaa ctaatattta
    13321 atactaatta aagtaaaata tggaggtata caaatatgaa acacataaaa atagcagcag
    13381 gattagcaca tgtaaattac ggtcatatat cagccgtagt taaagaagca atggatgcag
    13441 gtgtagacta cgtacattca gatgcagcag atatgcatga cttaaaaaat atgcaactta
    13501 tgggtggtca tcaaataata gaagcaataa gaccggtgac agataaacca atagaatgcc
    13561 atatatacac aagagattgt gatagattat ttatagaaaa aatagcagca gcaggatgta
    13621 acttgttgat aataccagca gaacattttt taggtgctcc tcttgcatac ataataaatt
    13681 actgtagaga atttggtatg aaagtaggat taacaattgg atgttacaca ccactatgtt
    13741 ttgttgatga agctatatat gatatagata gattgcaaat agtcgttcat ggtgttgatg
    13801 aaactgatgg aaaagataac tggggatgga gaaagagtgc tgttgattta gtaaaaagag
    13861 ctagaaaaat gatagacgaa aagaatccta agtgtgaatt agcaatagat ggtggactaa
    13921 gagcagacaa tatggagcca ttaatagaat gtaatccaga cgttgtaata ttatcttcag
    13981 caatattcaa ggataaagat ggtataacag caggtgttaa gaattgtaga aaagctatag
    14041 atgaagcagc tacaaagttt ggtttagaat aagataataa actatgataa aagagatgct
    14101 ttcttaatgt tttaaggaag cattaatttt atgaaaaact acaggaagct agactaattt
    14161 aattgatttt ttatactgaa acaatataat cataggaggt tattaaatga aaaaagaaat
    14221 gataaaagat tatttactga taacaatatc agcattttta atggcattag cagttaattt
    14281 tttctttgca gaacatacat tagctccagg agggataaca ggattatcag tcgtacttag
    14341 cacattctta tcaatgcctg ttgagaatat atcattagga atatctatgc cacttttagt
    14401 aatgggatta ttatttttag ggaaaggttt tggaataaaa acattatata taactcttat
    14461 gggcccatta ttcttaaaag taataccaca aactcacata actgacaatc tattagtagc
    14521 aggagtgata ggtgggttat tggtaggaac tgctataggg atagcaataa taaggggctg
    14581 tagtactggt ggaactgatt tagttgcaat gcttattaat aaggtaatta agtttctgaa
    14641 gttgccagtt atattattta tgctagatgg atttataata gtagcgtctg gaataataag
    14701 taagaatttt atgatatcta tatattcatt gatatcttta ttactcataa taaaaacaat
    14761 aggatttgta actacaaagt ttggtaagaa tactgaagaa attaataata taaataatgc
    14821 ttgtgctcaa taaaaataat aaaatttact ttaaatataa aagtttaaaa aatataaaag
    14881 cttaaataaa aagattaaat ataaaaaatg aaggtgtctc aaaatgaatt ttttagttca
    14941 tgaggcactt tttctatgaa atcaaatata tttttatcat ttggacaatg aaaaataggt
    15001 ttatctctat gttgagacaa gcctattttt gtttatttat aagataatat tttatttttt
    15061 tcagaatata gtttttttat aattgtacta agtctagaaa tatcattctt acttaaaaga
    15121 gcatttatat ttatcagttc agcattatgt tcaaagtcta caggagcagt ggttagtatt
    15181 aagtccacat catcaaagtt cgtaaatgaa atagaagata gaggtataga tttaactata
    15241 ttaatctgat taaattcaaa acttaacttt tggactaata aattagatac accatcacta
    15301 cagtgacata ttaaaatagt atttagggga gatttgcttc tatctattgc tgatattaaa
    15361 tataaagcaa gatatcctat ttcgtcatca ttgacttcat aattcatcaa ctgtttgata
    15421 acagtattag actctttagc agccttataa gtgtaaggaa atgttctctt tatatcattt
    15481 aatatgggat ttgtaatagt aaacccatag tttattctgt gtagaactga tttaaggtga
    15541 ttagcaagag actttattaa tattaaatca tcctttaagt ttaaatttag tatacttccc
    15601 caatgaacag ctattgcttc agatagatta tactctgtaa tttcttcatt tttaagaggt
    15661 tcaagagtag tagttttctt tattgattta agtaatcctt ctatatgaag aaggatataa
    15721 cagacctctt catcaataaa ttctacgtca aaagcttcac ttaggttagc acataaaatt
    15781 tttgattttt caaagtattc atgttctaat aattctctaa aaaaaccttc agataagtta
    15841 atatatcttt taattgaaat tcttttaata catatagcta tatgtattaa gaagtttata
    15901 taatactcat cactaaggtt acttttttct acttgtaagg tgtgtagagt gatttgagat
    15961 aatttctcaa aatcatagtt aaatagctcc ttaaatattt ttgttgatgg ggtatctttg
    16021 cattcattag atttcgagaa taatatttct ttaagttcag tataactttt attaaccgta
    16081 gcaagttcaa aaattgcttt tcgaatatct ttctcttttc cctctaaata tactccatta
    16141 ttggtttttc taactagagt cactttaaag ttttgcaaaa aattttgaat aactaccaag
    16201 tctttatgca cagtagctct actaacatgt agttcattgc aaatatcacg aatcctaaac
    16261 ttagaatttt taataaataa tttacttaga atataccttt ttctatcttc aggagagtat
    16321 gcatatatac aatttgattt atcattgata tctctaataa ctttaagttt tatatcttca
    16381 ttacctagaa taataacccc tgaaccagtt tttttatcta gggataaatt aaactctaaa
    16441 agccattcat ctaaaataac aaggtcatta cgaatagttt tattactaac ttttaattct
    16501 tcggcaatag tatctacagt aatatagtct gagctgttga gtaataattc agcaatatct
    16561 aataatcttt tgttcaatgc aaaccctcct aaatcttagt tactatttat taaccttgga
    16621 ccaatctctt tcaaaagtat tggcaccatc tattgtaagc tggttaaaag aagctttttt
    16681 aaataaatca tatgttacag ctgcataatc acatccacaa agagatgctt gagttaagtg
    16741 gaatacattt ttaatagaag ctgcaacaac tttagtatta tatccattta ttgatataag
    16801 gttacatatt tctgtaaggt tttttatacc atcattccca ttttcatcat ttctacctat
    16861 aaaagggaat aggtaatcag cacctgcatt tatagctaat gatgcttgat ttacattgaa
    16921 aatcaaggta catgcagttt ttacaccttt actatttaat atgtttataa gttctaagcc
    16981 aacttcagag aaatttattt ttattatagt gtttttattt atatttaata tcttatcaca
    17041 tgaattaatt aattcgtata aattatttcc aatgacttca gcagttaata tttctggatt
    17101 taaattagca catgtctcaa taaattcatg gaagtttact ttctctttaa gatacataga
    17161 tggattagcg gttattccct taaatccata ctttagacac ttttgaattt catcaagatt
    17221 agcactgtct atcaaatatt tcataattta attctccttt aagctatttg tcataaaaaa
    17281 gtgtatatac tctattttta ttattaagtc aattatacaa taagattgat tataaaatta
    17341 taacatgggt acttaataaa atttacataa tgatatggta ataataaaac attgattgtc
    17401 taataattac cataagcttt agtaaataag agttaacagt gaaggtattg ccttaaaaat
    17461 cctatgatat gtataatttg aatatgaagg caataattta agggaggaaa aatatgatta
    17521 acttttatca accatcaaga ataaattttg gacaaggaac tttaagtgaa ttacctagaa
    17581 taataggaaa atatggaaag aaatgtttgt tagttactac tccaaatgta gagccattag
    17641 acaagttata taaaagagtt acattgaatt taatagaaaa aggtatagaa gttgtacact
    17701 ttgattaaaa aatagctacg cacactcgtg actttagtca tgagtta
//
LOCUS       DAMJYX010000071        17605 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJYX010000071 DAMJYX010000000
VERSION     DAMJYX010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.072"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     misc_binding    149..380
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            488..1969
                     /locus_tag="PC479_002526"
     CDS             488..1969
                     /locus_tag="PC479_002526"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2805064.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1979..2872
                     /locus_tag="PC479_002527"
     CDS             1979..2872
                     /locus_tag="PC479_002527"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2805065.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2996..4057
                     /locus_tag="PC479_002528"
     CDS             2996..4057
                     /locus_tag="PC479_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2805066.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4212..4724)
                     /locus_tag="PC479_002529"
     CDS             complement(4212..4724)
                     /locus_tag="PC479_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2805067.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5244..6179
                     /locus_tag="PC479_002530"
     CDS             5244..6179
                     /locus_tag="PC479_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2805068.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6271..8142)
                     /locus_tag="PC479_002531"
     CDS             complement(6271..8142)
                     /locus_tag="PC479_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2805069.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8332..9258
                     /locus_tag="PC479_002532"
     CDS             8332..9258
                     /locus_tag="PC479_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2805070.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9397..9543
                     /locus_tag="PC479_002533"
     CDS             9397..9543
                     /locus_tag="PC479_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805071.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9648..10172)
                     /locus_tag="PC479_002534"
     CDS             complement(9648..10172)
                     /locus_tag="PC479_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805072.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10225..10449)
                     /locus_tag="PC479_002535"
     CDS             complement(10225..10449)
                     /locus_tag="PC479_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805073.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10646..11770
                     /locus_tag="PC479_002536"
     CDS             10646..11770
                     /locus_tag="PC479_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2805074.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12244..13068)
                     /locus_tag="PC479_002537"
     CDS             complement(12244..13068)
                     /locus_tag="PC479_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805075.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(13416..14471)
                     /gene="ispG"
                     /locus_tag="PC479_002538"
                     /gene_synonym="gcpE"
     CDS             complement(13416..14471)
                     /gene="ispG"
                     /locus_tag="PC479_002538"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2805076.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(14654..15658)
                     /gene="rseP"
                     /locus_tag="PC479_002539"
     CDS             complement(14654..15658)
                     /gene="rseP"
                     /locus_tag="PC479_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2805077.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(15671..16825)
                     /locus_tag="PC479_002540"
     CDS             complement(15671..16825)
                     /locus_tag="PC479_002540"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2805078.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(16989..17192)
                     /locus_tag="PC479_002541"
     CDS             complement(16989..17192)
                     /locus_tag="PC479_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2805079.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
BASE COUNT         6083 a         2720 c         2082 g         6720 t
ORIGIN      
        1 cttttaaaaa agttaaaaaa atatttttct aatactcaat ttgattacaa tttttttaca
       61 atttaaaaaa tattttttcc aaatttttat attaatactt gactttgtct gttttataaa
      121 ctagaataat taataatatt aagaatattg aaaggcattg attaggaaga gtaaatctga
      181 atcgattacc agagaaggaa actactagct gaaatgtttc cctaattaga aggattgaaa
      241 actacctagg agcttctatc cgaagttatc ttaatgattt tagtaggaat agacgttttt
      301 attacttgcg ttaaagtatc aagtggattt tttaaaatca atttgggtgg aaccgcggga
      361 aacagattct cgtccctttt ttagggatga gggtcttttt ttattaccta aattcagttt
      421 ttaaaatatt ctgtatctaa tttaattagc taattaaaaa tttaaaatat atggaggatt
      481 ataaaaaatg tattattcaa gtacgagagg caccgaagaa aaagtaactg catcacaagc
      541 aataataaaa ggaatatcaa atgatggtgg tctttatgtt ccaagtgaat ttcccaatgt
      601 aaaaaatgaa cttataaatt tagtaaattt aacgtattct cagatagcat tttttgtact
      661 tagtaaattt ttatgtgatt ttactgaaga tgaaattaaa aattgtatag aaaatgcata
      721 tgatgaaaag tttgattgta gtagtatagc tccattaaat aaagtaaatg atacttattt
      781 cttagaacta tatcacggtc caactttagc atttaaagat atggcactta ctataatgcc
      841 tcatcttctt aaaacatcta taaagaaaga taatttagag aaagatgttg tgattcttac
      901 tgctacatct ggagatacag gtaaagctgc acttgaggga tttaaagata tagataaaat
      961 aaagataatt gtattcttcc cagaagatgg tgtaagccca gtacaaaaac ttcaaatgaa
     1021 aactcaaact ggaaaaaata catatgtagt tggtataaaa ggaaattttg atgatgcaca
     1081 atcaggtgta aagaagattt tttctgataa agaatttaat caaaaactat tggataataa
     1141 ttatatatta tcttctgcaa attcaattaa tataggaaga cttcttccac aagttgcata
     1201 ctatttttat tcatatatga aattagttag caatagtgat ataaatttaa atgataaaat
     1261 aaattttgtt gtcccaactg gtaattttgg taatattcta gctggatatt atgctaaaca
     1321 aatgggtctt ccaataaaca aattaatatg tgcatcaaat gataataatg ttctttatga
     1381 tttctttaaa actggtgtat atgataaaaa tagaacattg aaattaacta cttctccttc
     1441 tatggatata ttaatttcaa gtaatttaga acgattactg tttgaaatat caaatagaaa
     1501 tactgatatt gtaaataagc ttttatcaga ccttagtgat aaaggagtct acaaaataag
     1561 tgatgatatg gaaaaaaatt tagcctcatt ctatggagaa tattcaaatg aagaagaagt
     1621 taaaagcacg ataaacaatg tgtttaaaaa ttataattat ttgatagata ctcatactgc
     1681 tgttgcatac aattgttatg aaaaatataa aaaagaaact tctgataaca caaaaactgt
     1741 tattgtaagt actgcaagtc cattcaagtt ttctgaagat gttctaaagt ctatagattg
     1801 tgattttaaa gaccttgatg actttgctat aattgataga ttatcaagta tttctaaaat
     1861 agatattcca aaacctatta aaaatcttaa aaatgcagaa atactacata aagatattta
     1921 tgaaaaagat gaattaaaac tagctataaa aaaattctta aaggtatagg tgattattat
     1981 gttagagatt atagttcctg caacaagtgc taatataggt ccaggatttg attgtcttgg
     2041 aattgcgtta aatatataca ataaatttta tgttgaagaa attgaaagtg gtcttgaaat
     2101 agaaggttgt gaagatgctt acaaaaacga aaacaactta gtttatacat ctatgaagta
     2161 cttttttgat agagttaaac cagaaaaaat acctgctggt atcaaaataa aaatccaaag
     2221 tgaagtacct atatgtagag gtcttggcag tagtgcctct tgtatagtag ctggagttat
     2281 agctgcaaat gctctatcag gtgctaattt agataaaaat caattactaa atatagcttc
     2341 agaaatagaa ggtcacccag ataatgttgc tcctgctatt ttaggaaata tgattgtatc
     2401 tgttactgat aatgagaata tacattatga tattataaaa attcctgaag aacttaaatt
     2461 ttgtgcaatg atacctaatt ttaagttatc aacagaaaaa gctagaggtg ttttaccaaa
     2521 ggaaatacca tattctgatg gggtattcaa tgttagtaga gttgctttac taatatctgc
     2581 cttactaaac aaaaactttg acctacttaa agtagcttgt caagacaaat tgcatcaaga
     2641 ttatagagga actctaattg agaactacaa tgatattgtt gaaaaatctg aacaattaaa
     2701 tagtattgga gtatttttaa gtggagctgg acctactata atgtctttaa taaaagaaaa
     2761 tgatgatagt tttgtggata atatgaaaaa ttacctacaa aaactaaaat ctgattggga
     2821 aataaaagag ttatgttgcg attctaatgg agctgtttta aatataatat aattatcgtc
     2881 ttctatacaa aatataacta ctcttttatt ataatttgta gtataataat taggactagg
     2941 taataatatt aagtattata atctagttct ttaaacatgt ttacattgag gagggattat
     3001 cataaaagag ttaataatta gagatttagt tgcaaaagtt cctataattc aaggtggaat
     3061 gggcgttggt atatctaggt catcactagc tggtgcagtt gctaagcttg gtggtgttgg
     3121 tgtaatttct ggagttcaaa ttggatatga tgaggaagat tttgaaacta atactataaa
     3181 tgcaaattta agagctataa aaaaacatat ttctaaggcc aaagaaatct caaatggtgg
     3241 tattattggt attaatttta tggtagctat gaaagaatat gaaacatacg taaaggaagc
     3301 tgttaaagct ggtgtagacc ttataatatc tggagctgga ttacctaata agctaccatc
     3361 attagtaaag ggaagtaatg taaaaatagc acccatagtt tcaactgcaa aagctgctaa
     3421 tgtaatttta aaaatgtggg atagaaaaga gaaaactact gctgacttaa tagttgttga
     3481 aggtccaaaa gctggtggtc accttggata ctctaacgag gaacttgata acatagattc
     3541 tatagactat gataaggaat ttgttgaaat tttaaaggtt gcaaatactt atggagaaaa
     3601 atttggtaga aacataccag tagttgctgc tggtggtata acttctagta gtgatgttaa
     3661 aaaatatata gatatgggtg caagtggtgt acaagttggt acaagatttg ttgctactta
     3721 cgaatgtgat gcccatgaga actttaaaat ggcatacata aatgctagtg aagaagatgt
     3781 tcatatagtt aaaagtcctg taggtcttcc tggacgcgcc ataagaaaca aatttataga
     3841 agaagttaaa ataaatcgtc cagaaataaa gaagtgctat aattgtttga ttccttgtaa
     3901 tccaaaagaa actccttact gtatttctca agctctaatc aatgcagtta aaggagatgt
     3961 tgaaaattca cttttatttt gtggaaatga tgcctataaa attgataaat taagtactgt
     4021 tgaagatgtt atcaatgaat tgatttctga attataattt aaagaaaact aaattaaaat
     4081 tttatcaagc taaggagttt aaatacgtta tttattaatc tttataaaca tatttatgtc
     4141 cttagctttt ttgtgtcaat ttccttttat ggaaatttta atttaattag agatagtatt
     4201 aacctttata actaatcttc ttttacaagt acattggtta tttcagctat atataaagta
     4261 tgataatctt tttgtggata ccatcttcca tctatacctt cagcaataaa acattgaggt
     4321 tcaagtttct gactatacat ttttttgcat ataatagcca ttttagattc tttaaaataa
     4381 ggtgtatcat ttaagtgttc tatattaaat ccaatctgtg atatcttatc ctcttctcta
     4441 ccagatacct ttccacaata tgaaagctct ttacgaaaat cttcatcaaa aaatgtaaga
     4501 gaaaatttat ctgtattatc tataaattcc ttagtataac gctgtggtct tattactaca
     4561 aaagcaacat cttttcccca cataactcca agccctcccc agctagcagt cattgtattc
     4621 actttacctt cattttctgc tgttataagt gtccaatctt tacctatcaa tccaaatgga
     4681 ttatttttaa tctcttctgc caaaatctct ttaaaatcac tcatatctct aataacctct
     4741 cattctctta tatttatatt ccttttattt tcgtgataaa ttataaatga agtctagttt
     4801 tgaaaataaa atactttttt aaatatatta ttgcaaataa gtaaatatat atatactctc
     4861 ttatttttta atatatttaa taatttcact cagaatatat atattcttta ctcaaattaa
     4921 atataactta tatactttat gtgtaaatta tggtaaaaat attatttaat caaatcaaaa
     4981 cttgatttat atacctggaa aaggtattat attaaagagt gcttaaaata cgcatttcat
     5041 aaatagctat atataaaata ataaataagg agagtgatgg gcaatggata ctagtccgtt
     5101 gcggagagaa ttttttacat tttcatcact taataatatc taaaatcaaa actaatacat
     5161 atattcagtt atataaatta gttaattaat agatactatt aaatgatgag atttatataa
     5221 aactgaataa aggatgtgtt gccatgataa gatattacaa aactatagat tcaaaattag
     5281 agaaacttag tttttttgaa gatggctgtt ggataaatct tgttgaacct aatcatagtg
     5341 aaatcaacga aatttctaat ttattaaata tagatgtaga aagcatagaa tctgcactag
     5401 atgaagaaga acgctcaaga atagatgttg aagacaatca tactcttata cttatagata
     5461 taccagtaga cgaaagtgac tctaattctt ctcattatac tacaataccc ttaggtatta
     5521 tactaactga agaagctatt gtaacagttt gtgatgcaca aactaagatt ttaaatgact
     5581 ttatagttgg tcatattaaa gattttttta cgtttaaaaa gactcgcttt ttgcttcaaa
     5641 ttcttcacaa aaatgcagct tattacttgc attacttaag aaaaataaat aaaatgacaa
     5701 tcataataga gcgagaaatt tacaaatcaa tgaaaaacaa agaattggtt caattattgg
     5761 aattagaaaa atctttggta tacttctcta cctcactaaa atctaatgaa cttgttttaa
     5821 acaaaatggt tagaactgct ggtataaaga agtatccaga tgatgaagac ctattagaag
     5881 atgttatagt tgaaaataga caggctcttg atatggctaa aatatatgga gatatattga
     5941 gtagaattat ggatgctttc agtgcaatta taagtaataa tcagaataat gttatgcaaa
     6001 ttttaacagt agttacacta attttctcaa tacctacaat aatatcagga ttctttggta
     6061 tgaatgtcat caacatgcca ttttctaata acccaaacgg attttggata atacttctta
     6121 ttacctctat aatatgtata gtgattacat ttttcatgtc aagaaataag ctattataaa
     6181 atattaataa aaaggccatt agaattaatt gatttacatt tatcctaatg gcttttatat
     6241 atttaaatct atataatctt ataatacttt ttaactatca tgttcttcat ctataatcat
     6301 ttcagtttca agcccttcta gcattgaatc taaactttct tttatattta gtatataaat
     6361 tggtacatac tcttcttcat ctattacctc ttcttgcaac ttaaatggag gaattaatac
     6421 ttcatcaaag tctatattta aatttggaaa gccttctact atctcttcac aattatcgta
     6481 ctcttcattt gctaaagaaa tgtacatatc taggctcttt ttataataaa gacctgcaag
     6541 catatactca cacttcttac ttccatattt aataccttct ttataatatt tctcagcttc
     6601 tgcgtcatca tttaatttta aataaatttg acctaaatta tatattgatt caacacaacc
     6661 ttgtgctcta gccttttcat accaaaattt agaatttaca taatctttat agaacatatc
     6721 ataaattaat cctaccatat gttgagcata cacattattt tcattggcag gtgtatcaaa
     6781 catcatttta gactcttcca atttaccttc tctaaaataa attctaccta gatgtatacg
     6841 agcattcata tggtttttgc tacttgccat ttcaaaatac tttctagcat tttttgtatt
     6901 tcctaagctc tcatatatac accccaatct ataactagac ctcgtatctc cattttcaga
     6961 tgctttctta taccaactta ttgcttcttc aatattattt aaatcttcat agatatttcc
     7021 taatttgatt tgacaagata cattatttgg aatcctcgaa taatacgaaa tcgctttttc
     7081 tatatcttga ttctgataat ataaatcccc tagattttca agggaacttt tgcatccaac
     7141 agctatagca tcctcataat atttaatagc attttcataa tctttttctt caaaatatat
     7201 acctgccaga ttattttttg cttctgttat atcatcttca gcagccattt tataataagt
     7261 ttttgccttc tctatctcat tttctctatt atataaatta gcaagtctat acttagaaag
     7321 tgtatgattt ttttctattg ctttctcata ataataaatt gcatctccca gctcttcttt
     7381 tctatcatat acatatgcaa gcctatactc agacttcaca tcatctctat cagcactctt
     7441 tttataatac ttctctgcat cttctaaatt atcatttatt tccataatca tacccaagtt
     7501 aaagcaacct ttcatatgac ctgacttgta caactgttta tataaactta ctgttgattt
     7561 attatctttc ttttttccat ttaatattgc taaattatat tttgcatcat catctaaaag
     7621 cacagaacct tttttatagt attcatatgc ttcatcatct ttctccaaaa aagtatataa
     7681 tacccctaaa ttaaaacaag ctttacctaa attcttgtct gctgctgttt ttaaccattt
     7741 ttcacacctt acaaaatctt tcattttaaa atggcataca cctaaattat tttgtgcttg
     7801 taaataacct aaatttgcta tcttttcata ccaatatata gcatattcat agccatctaa
     7861 cttatagtac atattaccta aatcaaaagc agcatcttca cttccacttt tatatgccat
     7921 cgaataccac ttttctgctt ctttaaattg ttttttactt tcatatatat ttcctaaagt
     7981 atattgagat tttacgtaat tatccaatgc agcactctta tacaactcta ttgcgctttc
     8041 catcttaccc atcttttcaa ggagggcacc cttagtatgt acgactttcg ggtcattaga
     8101 tatatttttt atttttttta tagaaaattt tgtatttttc attattcgct acctacccat
     8161 tttataaaat atattctctc aaacattatt atacattata atctacaatt aatgctataa
     8221 tattgaattt ttaccttttg tcactatatt gtaacattaa attttaaatt ttataatcat
     8281 tagattttat tatgtaattg gtatataatg tatatatagg ggggacattt tatgaatttc
     8341 agcaatattt ttatccaagt tgcagtttta tttataatta ttttagttgg atattttgtc
     8401 agaaaattca acttattaga tgaccactgc acatctaagc tttcaacttt gacaatgaca
     8461 gtttttttac catcaatgat aattagctcc atgcaaatta actttgatag taaaatgata
     8521 caaaaaatac tattgttatt atttacatct ttgattatgt atatagtttc aattataatt
     8581 gcatttttat tgaaatacat tttaaaatgt aatgataaaa aagatttggg aatataccag
     8641 tatatagttg ttttttcaaa tgttgctttt atgggttatc ctgtaattga agcagtttta
     8701 ggtcatgaag caatattcta tactgcaata ttcaatttgc cttttaactt attttctttc
     8761 actttaggaa tttatctttt atctaaaggc agtacaagta gaggtttttc aataaaatca
     8821 cttataagcc ctgctacaat agctattgtt attggacttt ttttatttgt aacaggactt
     8881 aggttacccc aatttataaa cgagccactt gagatgttgg gaagtataac tacaccaata
     8941 tccatgatta tcattggaag cttacttgct aattcttcag ctctcgattg ttttgtcaac
     9001 aaaaaattat atatagtcac ttttataaga ttattggtac ttcctgtgat tgtgtatttt
     9061 attctaaaag gatggataaa tgataagatg attttagcta taccagtagt aatttcttca
     9121 atgcctgctg ctgcaaatac tgctataatg gctaatcaat atgactccaa tataacacta
     9181 gcttcacaat gtgtgttttt tacaacacta ttttcagtta tatctatacc ttttataagt
     9241 attttactct tatcttgatt tattttttta tgtattatat aataacatat tcaataaatt
     9301 acaaatataa acattttatt gtagtatatt gatggaattt tttcctagat atttgtcaat
     9361 aatatctata tgtattatag aataggaggc ttaaatatgg atatggattt actctttaaa
     9421 atagtatttg ctatagttgc tataggtgtt tctgtaaaaa tcattaaaac tgttgctagt
     9481 ttagctttta aaatagcgtt gatattgcta ataattttat ttgtatatag aatgtttttg
     9541 taaacattta aaaataagtt ttttaacaca atagagaagt aaaatagatt atctttaatg
     9601 taaatttttt attaaagaca ctctatttta cttctctatt agcttcttta aattaaattt
     9661 ttattatatc ttcccataat ataaacatct tcaaattttc catctctgat tacagcatat
     9721 tttttagttc cctctatttc aaaacctagc tttttatata aatttaatcc cttttcatta
     9781 tccacaatta cttctaatcc tactctagtt aacataatcc aattatctgc tatatctaaa
     9841 agttgttcca taagcttctt acctactcct cttccctgat aattctctgc aacacttatt
     9901 cctatttctg cgctatgtct caatcgtgga gaagaattta catgtaaccc agcaaaacct
     9961 attacatctt tttctccatt attattagtt tcagctacaa acatataatc attttctccc
    10021 aaagaagcta acatagcttc agttttatcg actctgtcac tcattaatgc caaagtattt
    10081 ctcataacac tgtatgttct tctaatttca ttaaaagctt gtgcatcttc tcttcttata
    10141 ggtcttattg ttatatctat aggcttactc atattggatt ccccctcaaa cataatcttt
    10201 atttttaatt aaaattttac atctttattt tatactgttc aaatctatgt atgcttctac
    10261 ctgaggtaca tatatcatat tatcttttat atgtgtactt ccaaattctt ttaattctgt
    10321 accttcttta tccttagtta ttttatatct aatggattcc ataccttcaa ctacttcagc
    10381 atgaaaaaac ataccttcat attgtttaaa tctaaaatta aaattgtctt tagtaagttt
    10441 tattatcata tttttatcta ctccttatat aattgatatt tttcttatta ttagttgtat
    10501 atatactgct tattcatatt aatatattat aacttatcat ttatttagta taaagttata
    10561 aatagatata tttatactta ttttacaatc tttcttcaat tgatatataa tatgtttata
    10621 taaactaatg tatgtgaggt gaaatatgag caaaaaaaca aaatattttt ctttaattct
    10681 ctttcctatt gctttgcttt taaattttat agccagtaaa gttccaaata ttgttgaaaa
    10741 atattactcc caattcatag ataaaataat tgtacaaata ttgagtaaag tttctggaat
    10801 tttcccattt tctctctatg agattacaat gtacataata gttatatcta ccttcttatt
    10861 tttatgttgt actttatcta caatttttaa taagaaaaaa aaattaaaca tatatttaaa
    10921 aaattccatt ttaaatatat tatccattgt atcaatagtc tactttcttt ttgtagtgct
    10981 ttggggaata aactataata ggattccact agaaaccaca cttataaata actataattt
    11041 aaaaaacaat acctctatcc aatcaaaaca atatagtgtt aaagagctta ctaaattata
    11101 taaattcctt gtaattaaag caaatgaaac tagaaaactc acattacaag ataaaaatgg
    11161 tattgtaaaa tctaatacag attataatgg tgtcataaat agagctcaac taggatacga
    11221 taatatcttg gatatattac ctggtgttag tgatagttat tctaagccta agtatattat
    11281 ttcatcaaat ctaatgtgct atacaggaat aactggtata tactttcctt ttacaggtga
    11341 ggcaaatgta aatatagcaa tacctgactt atatattcct tgtacagttg aacatgaaat
    11401 ggcacatcaa aggggatttg ctagtgaaga tgaagctaat tttatagcgt atcttacttc
    11461 tataaaacac cccaatattg attttaacta ttctggatat attttagctt taaattatac
    11521 tgcttctgct ttaagtaagg ttgattataa tgcatatgtt gatatttctg ctggcatttc
    11581 agattctgtc cgtagagatt taaaaaatga aagtgaattt tggcaaaaat atgaaggtaa
    11641 aattaatgaa atttctaatg agtttaacaa ttcttactta aaagctaatg gcgttactga
    11701 aggtactcag agttatggta aaatggttga tttattgcta acttattatg aattatatcc
    11761 atataactaa acactacaat tcaaaatttt attcatatac agctgatatt aaatcagctg
    11821 ttttctattt aaataaatta tttaacttca agcaatctta tataatttga tagtacttat
    11881 actatctgta taacatttca attctaacta ttttagttaa aacatttcta ttatcttttt
    11941 taataaatgt tttaactaaa atttttaaaa cataaaactt ccacattatt tcactatcta
    12001 tataaaaatc taaaaattca catataaaat tacataacta aaaaatcata aatttataga
    12061 aaacatatat tcaaaataaa aaagcatgta tcaaaacttg acacatgctt ataatcttat
    12121 gtatatatat cattttgtca caataaaaat ttacttgtaa cactgatatt tttaaacaaa
    12181 atagtcctta tttaattaaa ttaacattaa ttaaattcgt actcattttg ctatcattaa
    12241 acattactta tagtaatttt ctaatacact atttgaaact tccttccctt gaaaactttt
    12301 caaccaattc tcatatgctg agtttttcca tacactacca tcattaaatg ttacttgttt
    12361 tacacaagat ataatgtact ttacaccatg ctctccagtt gattgtttcc atttatcaaa
    12421 caatagaaag gcatcatttt gattcttacc tggtgctatt gcattttgct ttgcatattc
    12481 tttctccatt tgtttccatt ctttcaacca attttctacc catgccttgc cttgttgttt
    12541 ttcaaacata cttattatat cctgtggtgg tgattgttgc atttttctat aagtatgtga
    12601 atatgatttt ggagacacag gagatatgcc tgtaacaata ccatagtcta caccagctgg
    12661 gctaaatcca acacttccta cttcgccatc tgctgccaca ttttgagcat cccaatatag
    12721 ctcaagtggt tttccatttt tatcatatgc taagcatgta atttcataat ccataattgc
    12781 cttttcactg ctatttgtta agcttataac attgctaggc caatgatatg gagtatcatc
    12841 atactgtatt ttgctattat ttataaataa tggaatattc tcattataaa caagaccttt
    12901 actactttga atatctgata ccttctttgt gacagtactt accgtatttg ctaagtatac
    12961 atttgttttt tctgaattgg tcgtactaag agaaaatgtt gttgtcgttc cagtaatcag
    13021 caaagcaaca actaaaatag gtaagctaga tattttttta tatctcacaa ttcttcacct
    13081 ctttttctat taacttataa taaaacaatt taccaaaaca ttaaaatcta tgttaacata
    13141 atttaccaac tgttctagta actatatcct atcatagtta ctatcaagtt tcaatactat
    13201 tgatggaaat tttcactata ttaaatcttt ttttaatttt ataaatttac aatatgattt
    13261 aactcataca tatatagtca aattagaata taagtttctt acatttaatg aagtttataa
    13321 aataaaaaat gactagataa ttaaatactg taaataaaaa cttattacaa aagtatttaa
    13381 aaatctagtc atttaatata tatatgaatt gtatactata atttttcaat ctcttctagc
    13441 aactcatcaa ctaatttgtt accatcaatc attctaacta tttcaccctt tttaaataaa
    13501 agaccttcac ctttaccccc agcaatacct atatctgctt ctctggcttc accaggtcca
    13561 tttacagcac agcccataat tgcaacagtg atattttttt ccatactacc aatcttttct
    13621 tcaacctcat ttacaacact aattaagtct atattacatc taccacatgt agggcatgat
    13681 ataactttaa tcttatcatt taaaagtccc aaacttctta aaatctgttt accaacaata
    13741 acctcttcaa cagggtcgcc tgttaaagat attctaacag tatctccaat tcctttaaga
    13801 agaagtgctc ctacccctat agaagacttt attgttcctt tgtgaacact tccagattct
    13861 gtaattccaa tatgaagagg ataatcaact ttttttgata ttaattcata tgcatcaaca
    13921 gttttataaa tatcggatga ttttagagat ataactatat tatgaaaatc taagtcttca
    13981 agaatcttaa tatgtcccat tgcactttca actaatgctt cagcagttgc agagccatac
    14041 ttttttaaaa gttctttctc taaagaacct ccattaactc caattctaat ttttaaattt
    14101 ctttccttgc atttctcaac taccatctta actctctcta tacttccaat attaccagga
    14161 tttattctta ttccatccac accttgttct atagcttcaa gggctagctt ataatcgaaa
    14221 tgtatatctg caataattgg tatatttaca ctagacttta tctttgctat atttttagca
    14281 gctttaatat caggaacagc aactctaact atatcacaac cagcttcctc taatctcttt
    14341 atttgagcta ttgtagcatc tgcatctctt gtatcagtat ttgtcataga ttgtatactt
    14401 ataggatttt ctcctcctat ttttacagct cctacactta cttctctcgt ctttcttctc
    14461 tcgtaactca tattccacct ctatatatta ttttatattg aatctcttta aatattaaaa
    14521 tattataacc tacatgtata ttataaatta aaaagctatc tagttaaata agaataaatt
    14581 tcatatataa aaattaattt attcttattt aaaactacat agcctatttt cttaaaatct
    14641 ctaaaaaact aaatcattga aataatctta gtatatcttt atatgtgata aaaagcataa
    14701 ataacattaa agctccaaaa ccaacaacat ttatcatgcc ttctttattt gggtcgagtt
    14761 ttttaccacc tctaacagct tctagtaaca acataagtat tctccatcca tcaagtgctg
    14821 gtattggtaa tagatttact atacctaaat ttaagcttat gacagctgct aaatacacta
    14881 catttatgat gcctgtacga gcagcatctg ataccatacc aataacacct actggtcctg
    14941 ctacagcatt gccaatccct cctggtacac gtcctgtaat catttgacct agaaatgtta
    15001 gcatctgttt tgtcatatct acagtagttt tacatgcatt tactattgac cctaaaaagt
    15061 ctttttctct ttgtggttga attcctattt catactttcc attgttattt ttaggtgtta
    15121 catttacaac tttatcactt ccatctctag ttatactaag ttttaattct ccaccagaag
    15181 aagtgtttat tatatttgca acttcatccc aagtcttaac agaattacca ttaattttat
    15241 ttatcttatc tcctacttga agccctacag cctgtgcagg agtatctggc attatctttc
    15301 ctaatttagt cgttggagaa cctatataca aaaatactgg aactaaaagt attgctgcta
    15361 atattatatt gaatattggt cctgctacaa tagtactaaa tctttgtaat atagacttgt
    15421 ttccaaaaga atttggactt atttgttctt catcttcacc ttccatgctg acatatccac
    15481 caattggaag tgctcttata gaatactctg tatctttttt tacactatat atcttaggcc
    15541 ccatacctat agaaaactca tttactttga taccacttct ttttgcaaat ataaaatgtc
    15601 ctaactcatg tattaataca attatactaa atagtattaa tgcggctatt atagtcataa
    15661 attcaccttc ctattttttt atcagatttg caacataagc tctactccat ctatctgttt
    15721 ctaagatttc ttctaatgtt ggttcactta tgctactatg aacttctaaa gtcttctcta
    15781 tgtaatatgg tatgtcatag aaaccaattt tatcctctaa aaattcactt actaaaactt
    15841 catttgctga atttaaaaca gaagaataag ttcctcccat ttttaaacac tcatatgcca
    15901 attttaaaca tggaaaagtt tctaaatctg gttcttcaaa agttaaagta ctaaattttg
    15961 aaaaattcat tctttcaaag ctactttcca ttctatctgg atatgtaaga gcatactgta
    16021 ttggtagtct catatctgga catcctaatt gagcaattat tgagctatct gtatactgca
    16081 ccattgaatg aattatgctt tgtgggtgaa ctactacatc tatattttct tgttctactc
    16141 caaataacca tctagcttct atcacttcaa gtcctttatt cataagcgtt gaagaatcaa
    16201 tacttatttt tcttcccata ctccaatttg gatgcttcaa agcttcattt ttagttatat
    16261 ttgcaagttc tccctttttc tttcctctaa atggaccacc agatgctgta agtattatct
    16321 tttctatatt ttttttattt tctccattta gacattgaaa tatagcacta tgttcactat
    16381 caacaggaag aatatttaca ttgtattttt ctgcttcttt cattacaagt tcaccagcag
    16441 ttaccaacgt ttctttattc gctaatgcta tgtctattcc cttctttata gcacaaagag
    16501 ttgggactaa tcctatcata cctactacag cagttaaaag gacatccact tcatcaagtg
    16561 atgatattat ttttaatcct tccattcctg ctaaaacttc tatttctata tttttaggca
    16621 acatttcttt taattttaat gctttatttt cctcaaaaac tgttacatat ttaggtttaa
    16681 actctactat ctgttctagc aataattcaa tattactgtt agctgatatt gcaactatct
    16741 caaacttatc tctatgttct ctaaccacat ccaaagtttg tttacctata gagcctgtag
    16801 aacctaaaat tgatatcttt ttcatataat aatcctttct aataataaat ataccctgtc
    16861 aaaacattat aacactacta aaacaattat ttttactatt catagaaatt tcatatatta
    16921 ttccacagat taaaattaat ctattatttt ttgtattttg tggaatatta taaaatattc
    16981 tagtattatt aaatgccctt ttttatttcc acaacatctt ctattttaca gtctagtgct
    17041 aaacatatct tctccattgt atccatagaa acagatttat catttcctag tctgcctaaa
    17101 acagaaatac ttatacctga tatatttaca aattccattt tctttatttt tctgtcaatt
    17161 aacaatttcc ataatggagc gtatgaaatc atgttatcac ctcgcaaaat atattctatc
    17221 acattttatc tattttggaa aattttcatc tatttagata aataatcacc tgcttaagtg
    17281 tcaaataatt ttatccttct aatatgaaaa tttaatacta tactaaatga ttggtatata
    17341 acacatatga tgcattatta ttaacatata aaaattttta ttggtgctaa attaaagtaa
    17401 tatactatta ttatatttgc agatatataa gaaagatttt attatatatt taaagtactt
    17461 gattataata agagacaagg gctatgatgg atactataga aagttagaac tatactattt
    17521 tagaatgaac aattaaaata attaaaatta gtttaatctt ttattcttga taaaatcata
    17581 ttaaataaat acaaaaagaa tctta
//
LOCUS       DAMJYX010000072        17539 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJYX010000072 DAMJYX010000000
VERSION     DAMJYX010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.073"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(149..769)
                     /locus_tag="PC479_002542"
     CDS             complement(149..769)
                     /locus_tag="PC479_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2805080.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(1083..3221)
                     /gene="vanT"
                     /locus_tag="PC479_002543"
     CDS             complement(1083..3221)
                     /gene="vanT"
                     /locus_tag="PC479_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2805081.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3239..4045)
                     /locus_tag="PC479_002544"
     CDS             complement(3239..4045)
                     /locus_tag="PC479_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2805082.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(4042..5142)
                     /gene="vanG"
                     /locus_tag="PC479_002545"
     CDS             complement(4042..5142)
                     /gene="vanG"
                     /locus_tag="PC479_002545"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2805083.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5403..6485)
                     /locus_tag="PC479_002546"
     CDS             complement(5403..6485)
                     /locus_tag="PC479_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2805084.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(6535..7236)
                     /locus_tag="PC479_002547"
     CDS             complement(6535..7236)
                     /locus_tag="PC479_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2805085.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7670..10201)
                     /locus_tag="PC479_002548"
     CDS             complement(7670..10201)
                     /locus_tag="PC479_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2805086.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10309..10926)
                     /locus_tag="PC479_002549"
     CDS             complement(10309..10926)
                     /locus_tag="PC479_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2805087.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(11288..12172)
                     /locus_tag="PC479_002550"
     CDS             complement(11288..12172)
                     /locus_tag="PC479_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2805088.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(12363..13613)
                     /locus_tag="PC479_002551"
     CDS             complement(12363..13613)
                     /locus_tag="PC479_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2805089.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(13835..14599)
                     /locus_tag="PC479_002552"
     CDS             complement(13835..14599)
                     /locus_tag="PC479_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805090.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(14593..15318)
                     /locus_tag="PC479_002553"
     CDS             complement(14593..15318)
                     /locus_tag="PC479_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805091.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(15320..15688)
                     /locus_tag="PC479_002554"
     CDS             complement(15320..15688)
                     /locus_tag="PC479_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805092.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(15864..17408)
                     /locus_tag="PC479_002555"
     CDS             complement(15864..17408)
                     /locus_tag="PC479_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2805093.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         6018 a         3088 c         1756 g         6677 t
ORIGIN      
        1 aacaatattt tgaactaagt ataaataagt tttatatatt ttagtttaca aaagaactaa
       61 aaattttgta ccttaataca aaaaataaaa cgtatcttaa aataatttag tactaaatca
      121 ttttaagata cgttttattt cgtacaagct aaatcatttt atcatcttta tcattgtgat
      181 gttttttatg atggaaatca taattatgat tttcaagatt tttatttact ctatttccaa
      241 tcttccatga caaaaacacc aataatataa atgatgctat ctttaaagga cttgttataa
      301 aaccttctat atcacttgcc acgtaactta taactataaa cattacaaat tttcctgcca
      361 acattgctgg cacaaaatct tttaaatctt ttttaccaaa agctgatgcg atagtaacta
      421 aacaaccggg cataaatgga caggcatacg ctataaataa taacttaaat ccctgtttat
      481 ccatccaact tattgctttt tcagttttac tatttctcaa cttattaaaa agcttactat
      541 cattaaatct gcttattaat aagaataatg aagttgtccc aagtcctgag ccaatccatg
      601 ataacaaaca tccaataaat aatccttgta ttgcagcatt tgctgtaact atagcaacaa
      661 gtggtaaggc tggtataaag ctttctataa atgcacttaa taagccgatt attgtggcta
      721 acacccaata attttgagtc aaatcaagta gttcttgtat atactgcata ttgcttttcc
      781 ccttttttaa ctttaactat tattatatac tctatatgtt tattatatac caatctcaaa
      841 aaatgaatct aatattaaaa agtaaataat ctatataagt ttacagttaa ggtataatta
      901 ttttattata tattataaca aaaaaggtga tgtttcaaaa taaaaatctg aaacatcacc
      961 tttttataac ttaaattgtc atgtaagtta taaaaaatat atattagaca taatattact
     1021 aaaaatgtct agtcagagta aaataaatta cacagtacaa caaatattac tatttaattg
     1081 agttacttct ttataacatt taaccttctt cctactctgc tcaataattc attcgatata
     1141 ctacatgagt tatttgcaac ttcagaagca gataattcag ataaattatc tcttcctatt
     1201 attattgccg tattaccaac ttctacatta ggtatatctg taatatcaac agcaagttgg
     1261 tccatgcaaa ttcgacccac tattgctgct ctacaaccat ttattattac gtaacttttt
     1321 ccacaggata aatttctagg tataccatcg gcatagccaa tagataatat agcaattacg
     1381 ctatctctgc ttgcaataaa gactcttcca tatccaaaac tatcatcttt ttgtattttt
     1441 ctaataagaa ttacttgtga ttttaaggat aaaacaggtc gtaaatcaag ttgcaattta
     1501 gtcgtatcat ttggagaact taatatcccg tataatgcaa ttccaattct tacataatta
     1561 cacttaagat tgggataatt aaaaaaacca taactacttt gaatatgtac tttaggaatt
     1621 tttatacctt tttttaacag aaaatcaatt aacttataaa aacattcaat ttgtttattt
     1681 gtaaagtcta catcttcatc tttcagacta tctgaaacac acaagtgtgt aaatatgcca
     1741 catatatcta agtattttaa tttaaatgcc tttaaaattg acctcacatc attaacatca
     1801 aaacccagtc tgtgcatacc tgtatcaatt ttaatgtgtg attttatgtt gtatttctgt
     1861 ttgtttaaag agatagcatg attaaggtca ataacactct gtgtaagatt gtatttatga
     1921 agttcctttg cccttattgg gttagtatat ccaagaatca atatttctcc tagtattcca
     1981 tatcgacgta aatcaatccc ctcatctatt gttgctactg caaatgcttt tacacctatt
     2041 tggtttgcac aagtcgctac ttcaaaagca ctatgaccat aggcattagc ttttacaact
     2101 gccatcaatt cacaatcttc tggcatagct ttttgtatca tctttacatt atgttccaaa
     2161 ttatttaaat caatttcaat ccaagctcta ttggtattta ccttatgttc tcctttcaag
     2221 tcttttttta gaggtttaag aatcaatata ataattatag aagctataaa agatgctata
     2281 cttaccacta tataatgaat aaggctattg tcaaccaaga cagtttgtat tccgaatatt
     2341 ttagaaaaca tacgaataac aacaatcatc ataggatgta taatataaat aattaatgat
     2401 gatgttcgca ataacctagc tcgagaacct ttcaaaaatg ttaaggctgt aaataagaaa
     2461 tacatacatg gcaataacat aatatacatg ctatcatgac gctgtacccc tagtttatgt
     2521 aaaaacattc cctcacataa cataagtaag aatgatataa gaaatccaat caagctattt
     2581 ttagttgaca tataattaga tttattagat attattccac caagtatgaa aaatacaggt
     2641 gcaaaaaata taccatttcg tgtataatct gatacttcaa aaatataccc atataaattc
     2701 tttaatagtg gtattttttc taaaaaacca taataactat caccaaacat accaattaca
     2761 taaataacaa tagtgattac tagtgagtac tttaaaccta gtctttttac aagaaagtat
     2821 gcaatacttg caccaataat tgatgcaggt agataccata aatgatataa agtcccatca
     2881 aatacaatgt ccttaataat attgggcact aaattattca tggaaaaata tccagtgtaa
     2941 atatttaagg gaatataaat tacaatagct atcccgtaaa taaataatac attttttaga
     3001 aaagatttca gtttattaca attataatta tatgttgaaa ttagaaaaaa gcctgatagc
     3061 ataaaaaaga atggcacagc aatacgagca attatactag ttaacacaaa gtcagccatt
     3121 ttactatatg ataataatgg agatgtatga attgcaatta taagtaatgc agctataatc
     3181 ctaaaataat caagccctgc ataagattta ttcctaatca ttcttgttcc tccatttctt
     3241 agtcctttct atttctccaa aatgtcacaa taaaaccatc cttattgtta cccgatattt
     3301 gatatacaca ttgttcaggt atagagattc tcacttcttc tttagtatga ataggcacat
     3361 aaaagatttc tataattttt tctttatctt ctatttttaa acaatttgta tacgaaaatc
     3421 tcttaatata ttctatatat tcttcaagag cgagattcat tttattgata atctcagaat
     3481 gcggatatcc cacataacga aaatgccatg gttcttgaga tattcctgtt atttcttctt
     3541 ttttacactg ataacgctct ataaaaccat aatgtagtgc tgccttacga aactcattac
     3601 aaataccatt ataaggaaat tcagggcaga taaaatctat aacttctttc tttaacccta
     3661 aatcaatagc aagccctgtc tgatgttcac tgtgatttgg aagtgctaca aactttttag
     3721 taaagtcttt tccattgtca cgcagagaac ttgaatatat atcttgttgt tcctctgcag
     3781 tacgatatcc acttacaggt actatttcat ttattccaca tatacttttt aaaatatttt
     3841 gtaaaatgtt tgtagcttca cgtttcataa gaatacttgg aaaccttgta tctacaggaa
     3901 ttaaatctat atcttcagat gttttaactg gtaaaaaagc atttactaga attagattcc
     3961 catcataaac tttatcttta gtcaataaga tagtatttgt cattttatct tctttatttt
     4021 tatctaataa aatattattc atcattttat gtacacctca attatcttgt ccaatatttc
     4081 ttcaaaagac aatccaatac ctttcatcat atttggataa cgactatgag aagtaaagcc
     4141 aggaattgta ttgacttcat taaaaataat ttcaccagat ggagttaaaa acatatctac
     4201 tctagcaaat ccagaacatc caagagttct ataaataatt tttgctgtct cttgtattct
     4261 tttctcacaa tccaaatcaa ttctagctgg catatgaatt tttgaacttt tcaatgtata
     4321 tttttctgta taatcaaaaa atccttttga caattctatt tcatctactc ttccaacaag
     4381 taagtcatcg tttcctaaaa ctgcacaccc tacctcaaaa ccatcaatat tttcctcaat
     4441 tattacttca ttatcatgtt caaaagccaa gtctactgct acttgtaact cttctttttt
     4501 ataaatttta ctaataccaa atgatgaacc tgcacgcaca ggttttataa ataatggata
     4561 agttaagtct tttgacagtt cagccacatt aaatttatct gagtatttta gtaatattga
     4621 ttttggtact ttaatacctt ctgaatgaac aagtttatgt gatttgtctt tgtccataca
     4681 taatgctgaa gaaagagtac cacatcctat aacctgtata cctgccaatt caaataaccc
     4741 ctgcactgtt ccatcttctc cattttttcc atgcagtaca ggaaacacaa aatcaagttt
     4801 agtgtattta atatcatttt cacatgattc aatagcacta tttggaatat gattctgaga
     4861 aacagcatca ggaattaaaa attcaataat accatttttg acacggttct gcgaaacaat
     4921 aactggaatt atatcagtat ccctttcaaa ccaagtgtta ttctggatat tattataatc
     4981 tccaatataa tgataccaat taccctccct tgtaattcca attggtataa tatcatattt
     5041 ttccgtattc aaattctcaa gtactgcaaa agctgattgt aatgatacat catattctgt
     5101 tgagtttcca ccaaatataa ctgcaatttt tttattattc atctttgatt ctcctttgtt
     5161 gatttattta tttttataca atctacagaa acatgttaaa atactttttc tatttcaaat
     5221 ccaatcaacc ttcacttaca attgcatctg ttccacataa tatattttca catttttcta
     5281 ttgatataat tactgtatca aaataaaagc actcttgttt tgatacagta attatatcaa
     5341 taacaagagt gctctttatt tatttatcct aaagaatttc ctattttttt atacttaaat
     5401 tcttacaaat ttcctacaat cggaataata accttaaaga taattatatt gttctcgcta
     5461 tgagctgtta ttgtcccgtt atgaagctcc actatttctt tagctatagc aagtcccaac
     5521 ccagctctac cactgtttga attacgagat gtatccaacc tgtaaaattg ttcaaaaata
     5581 cgctcaagtt tttcctctgg aatcgtattg ctatgattta taaatttaag ctttatataa
     5641 tcatcctcct gtacagctgt tatttcaata gtagtatctt caaaactata ataaacagca
     5701 tttcgtaata agttatcaaa tacacgttgc attttattaa catcacacct tatcatcata
     5761 tttggtgtaa cattcagtgt atattttaaa ttcttactca tcaaagtagg tttaaactca
     5821 tatgttagtt gttcaagcat acgagttaag cttactgtac tgtattcaag tgatatattt
     5881 gataaattaa accttgtaat ttcaaagaat tcattaatta aatcttcaag acgctctgcc
     5941 ttatccaacg aaatagataa atattttttt cgcagttctt cagaaatctg actttcatct
     6001 cgaagtagtg tcagataccc tataactgat gtaagtggtg tttttaaatc atgtgcaaga
     6061 taaacaataa gttcattctt acgttgttct gccatttgtg tctcaaattt acgttgttca
     6121 agtatatgtt taattgagtt tattttcttt tctattgcca ataactctgg agataaagcc
     6181 acttctccaa catcttcttt gataagagag tcaatacctt gatttatttc actaaaatat
     6241 tttgtaaacc aatttaaata aacccggaat attataaaaa acacaatcac catagctagt
     6301 atcatgaaga gctccatgct attacggaaa atctgctgat ataaattcaa tgcagcttta
     6361 tattccatac gaaaagcatt ttcaaagaat gatactatcc agatagaaaa cttaccatac
     6421 aatataaaag aatataaaat ccatatgaaa ataatggcta taaatgccat taaagctgtt
     6481 ctcataaaaa ttttatttct cacttgagta aagtcaggat tgactttaat atttttactt
     6541 ttcaattgta taacctactc cccatacagt ttttataaat ttcgggtttt tagaaggttc
     6601 atgaattttt tcacgtaatc gccctatatg agccataact gtgttattat tatcaagata
     6661 tttttctccc catacagctt caaacaattc ttcagaagga accacttttc cttgatgttc
     6721 acataaatac caaagaatag aaaattcaat cggtgttaat gctacttctt tcccaaataa
     6781 actacattta tgtgtttctt tattgataat caatccttta atatcatact cattaacaat
     6841 tatcgactgt tgttcataag aattattata tcgcatatat cttcttaatt gtgtctttac
     6901 tcttgcaaca acttccaaag ggttaaatgg ctttgttata taatcatcag cacctatggt
     6961 taaacccatt atcttgtcta tatcttcaat tttagctgtt aacataatga taggataata
     7021 gtatttttct cgaattttct ggcaaatatg aaatccatca atatcaggaa gcataatatc
     7081 taaaatagcc atatccaaat ttttagattc aatacattct agagcttcct tacctgtata
     7141 aaacttgtat actgtaaaac cttcattttt taaatatact tcaattaaat ctgctatttc
     7201 tttttcatca tccacaatca aaatattttc gttcatagat tatttttcac tctccttatt
     7261 atcgaggtca tcatttataa tattggtaaa tttacatatc aaataattac aagcatattt
     7321 gtaactaatt tttataagaa aagaactact acatcagaat gtgttgatgc tactattgga
     7381 acacaaaaac tgagattaat tgttattata aaaactcccc taacacgtcc ttattatcgc
     7441 ataacaggta ttaactttca ataaaaatat ctatcagtca aacacaattc aatttattat
     7501 accagagaca atccatataa aaaacaatta aacctatgta tgtaaccaca gacattacta
     7561 aaatttccat gttattttat ataaaaattc aaaaaaggag ttgaatttaa aatttgatta
     7621 gaaaaaaatc aattttgaga caactccttt ttattttaat gtatttaaat tatagtatat
     7681 cagccttcat aatatcctca aaatcacctt tttcagatat taatcttatc aatttaacag
     7741 tacagcttaa atcatttata agtatagctc caactaatga accatctttc ataaataatt
     7801 ttttatatat tctttgacct tcatcttttg atgaaatttc tttatcaaaa ttttgtatat
     7861 taccaatact aaatactttt acattcattg catctaatga aactggatat acttcacttt
     7921 caaatgtcaa attatctcca cttgcatttg cccctgctat ttttcccatt tctactgaag
     7981 atggccatat tgctaaacta atatttccca cttgtgcaac atctccacaa gcatatatat
     8041 ctttaacatt tgtctgtaat gtcttatcta caacaatacc tctatcaatt tttacagatg
     8101 tatcctgaac catttgagtg tttgctctta caccaattga aaatactacc atatcacagt
     8161 cgacactatc tccatcttta aatacaactt tttgagcttt tccatcacct tcaataccat
     8221 caactgctac acccaatctt acatctattc ctgtatctct tacacattgt tctaatattt
     8281 tactaccttc tttatcaagt tgttttgata gtatgctatc catggcttca actactacta
     8341 cttctaatcc ttttagtcta aattcccatg cagcttcaag tcccaataaa ccgccaccaa
     8401 tcactacaac tttcttagat ttttctagtg cttcttttac actatataaa tcttttatat
     8461 ttctaagagt aaacacattt tctaaatcat ggcccttaat tggtggtata aagtttctac
     8521 ttcctgtagc tattacaagc ttatcaaatt taatagctcc atcattcact ataatagttt
     8581 tattcacttc atctaattta tctacattcg ttcctaatat tactactata ttatttttat
     8641 cataccaatc tttatcttcc atgtagaaat ctgaaccaag ttcttcattt ataccatcag
     8701 aaagtgcagg tctatagtaa gtcaactcat cttcattaga aatcatagtt atcttacaag
     8761 ttttatttct ttttcttata gcatctgctg cataaaaacc agctgctcca tttcctacta
     8821 ttacgaaata ttcatcagta tcttttctaa aagtaacttc atcttcttca acttctataa
     8881 attggtcttc cttagctcca catacagggc atacactagg agcactatca ccttcaaaaa
     8941 cttcattaca aattacacat ttaaatttct tagttttctt tttacttcca aaagtagaat
     9001 ttaacttatc taggaattta cctgtataag aatataattt atcaatctct tcatttgatg
     9061 gtttaaagtt aacagcaaaa ggttttactg ctgtcattct aagctgactt attctctcca
     9121 tagcattttc tataccttct ccactccatc catatgaacc aaatactgaa gcaacttttc
     9181 caccatgaag aactgggttt aacgatatta atatatccca tataggcttt aacgcatctc
     9241 ctaatattgt tggtgttcca aataatacac cttgtgatac tgctattttt gccaaaatat
     9301 cttcttgctt atgctctatt acatcaaata aatttacttt atagttgcta tttttttcaa
     9361 tatatgcttt tatagcttct gccataatct ttgtatatcc atgtgcagaa acataacaaa
     9421 ttgtaacttc tttttctaac tttatttgtt catttacact ccagttgtaa taaaggtcta
     9481 taatttttct aggattttca gttaatactg gaccatgacc tggacataca gtatctatat
     9541 ctaaatcttt tatcttttct atagcagtta ccatagatgg tttaaatgga cccattatac
     9601 aatcataata gtatcttaaa gcatccaagt aatccttttc ttcattttca tcaagtgtat
     9661 ttactatctt atcattacta tagtgactac caaatgaatc acatgttaca agagttttat
     9721 cctcttttat atatgtgtat atggtatctg gccaatgaag cataggtaca gagaaaaatt
     9781 ctaaagtttt gtccccaatt gataatgtat ctccatctgt aactgcaaca tattcaaagt
     9841 ctttgtttac gatttcctta agatatttaa ttgctgtttc tgaagctact acttttgcat
     9901 ttttagctaa atccaaaagc ttttcaacgc ttcctgcatg gtctggttca gtatgactta
     9961 caacaatata gtctattttt tcaaaatcta tatttaaatc atttaatctt tcaatataat
    10021 tgtcaaaatg cttatcttta actgtctcaa ataaaactgt tttttctgtt ccttttaata
    10081 catatgaatt ataagtagtt ccataaggtg tatacataat tatgtcaaat actcttaaat
    10141 cagggtcaag cgaacctacc caatataaat tttcttttat ttttagtgat ttcaatgaca
    10201 taccgtactc ctcctaaaat agttatttag tctctttata ccctgcttgt tatattttaa
    10261 acaaaattta ttcaaaaaag aaattatttt aaagatagtt taaatcttct aatcttcttt
    10321 ttctaccttc aatacagttc cctttttaga atctactttt atgtcatatt catttttatc
    10381 tttatctatt cttattttat aaaaagtaac atctaaatct ttgtctaaac tccattcttt
    10441 aactattcca ttttgttctt ttaatgcaat ttccatagct tgttgaggtg tcattatgtc
    10501 atttaaatct aatttttctt ttctagcaac tccatttaaa tcttctgaat ccaacttttc
    10561 tgttttatct tctaatacat cttttgtatt agcatcaatt ttcatcttat attcattatt
    10621 atcatctaca ccttccacag tataaacaaa tttatttaaa tctctctcaa gacttaaatc
    10681 tactatatca gcatccttat atttatcttt aaatatgttt attgcatctg tgtatggcac
    10741 tttaaaatct tgttccatta aattttcatt atttttttca tcttttaaag cgttatttga
    10801 tgcatttgcc tccttttttg tattatcttg tgcttgtcct gaatccttag ttggactccc
    10861 actacatgca gttaataaac ccgctataag catacttacc actaataaat acgttttttt
    10921 acacataaaa accctcctta aatatattaa tttattctat attatgccct ctaaaccctt
    10981 tatttaccct tttatattaa aaattcctcc tattttatca attttaaaac tttatattta
    11041 ctaaaattaa agttacataa aaattatact gtctcaaaat ggaaacaagc ctctttttca
    11101 ttgttgaaat aataaaaata tattttattt cgtacaaaaa gagtgcctca tgaactaaaa
    11161 agttcatttt gagacactcc cattttttta tatttttact tacaaaataa cagtacttat
    11221 taatttttta caaaccttat aagtatatct atatgatatt cttaaattta aacactatat
    11281 taaaaaatta aattttgttt aattctttta ccgttaaatc cattttagga tatggaatcg
    11341 taattccttc ttcgtcaaac ctaactttaa cctcttctag taaatcaaga taaattgtcc
    11401 aataatcttc tgtcttgcac caaactctag ttgcaaattt aatagcatta tctccatgtt
    11461 ctactactcc tataaatggt tctggttcat ttattattaa tttatgatta ttaacaatat
    11521 ctttaagtac tcttctaaca tgtagtatat catcttcata ccctacacta aaagtcaaat
    11581 caactcgtct agtattcttt gctgaatagt ttattagact atcatttgat acagaaccat
    11641 taggtataag tatctgttta ttatctggag taaccaattg tgtataaaaa agacctattt
    11701 gttcaaccgt tcctccatga cctgctgctt ctacgtaatc tcctacttta aaaggtctaa
    11761 tcaatagtat aataaatcct ccagcaaaat ttgatagact accttgtaaa gccaaaccta
    11821 ttgctacccc tgctgatgct atcacagcag caaaactgga caaactcatt ccccaatatt
    11881 ccagaacaat gattataacg gcaactttaa gtacactcaa aagaagagac tttaaaaact
    11941 ttcttaaagt taaatcaata tctcttttac taagtaataa cataacatgg tttacaaatt
    12001 tttttattat tttaaatcca atagaaagta taagtatgcc tattataagt tttactccgc
    12061 ttgtagtagc ccattctagc agactttcca ttatacctcc catatccatc ttattaatat
    12121 ctaaattatt taaatctaac ttgcttatat catctatttt cgtcgtatcc ataaaaaact
    12181 ccttacctta tattttagaa ccaatattat agtttaaagt tcttattttc tatttaatat
    12241 aatacaatac catttgtaca tagtaaacaa gatttatatt ccaattatac atattaggaa
    12301 aatgcaaact atttgttata tatatgcaaa atataatact aaattaaact attaataaaa
    12361 cactatatag ccttaaaatc atcatttttt acccctatat gagatgatac tatagttata
    12421 atacttagta ctataaatac tacaaaacct tttttatatc catcaactat attatatgaa
    12481 tttacttgta aagagatagc tataatcaaa ttaataattc cacttcctat aaattcaaaa
    12541 gtattaataa cactagttgc cagtccagaa taaatttctt tatttttata cttggcatca
    12601 ttaaaaacct gtagatgtga cataactata catcccataa taaagaatga tattggtaaa
    12661 actataattg gtggttttac ctggtataca actacaatta caatccatat aaacaaatct
    12721 atcattgctc caaattttat tatattaaat ttgctagacc ttattttagc aaatacaaaa
    12781 tccataatta ttgaaccaaa tataaatcca tatgtaaaaa atgatactat aaacgcagaa
    12841 aaactcttac tcacaccata aacgtccatt atgtatctta ctccccacaa gcttataaat
    12901 gcacttgtaa gaccaacaaa agagaacatt atcattgaat tataccatgt tgatttgttc
    12961 tttataacag acttaatacc atcaacaata ttaaccttct ctgacttttc atagggttca
    13021 gtatctacat tgaatccata ctcttttggt gtatctctaa caataatata cataaaacat
    13081 ccaattacaa caccaattat accgattaat aaaaatgaat ttctccaacc aacaagttca
    13141 gacaaaaaca caagtggaaa agtagcaaga actccaccta aatttcctac aaatgaaaat
    13201 ttagctgttg cagaagcaaa ttctgactta ttaaaccatc tcccttgaat ttttaaaata
    13261 caaagtaata ttactgatgt tcctgcccct acaattacac gagaaatata tgctaattct
    13321 atactttgaa tcaaaccaaa taaaattgac cctaaacttg ccattattat tccaagactg
    13381 ctaatttttc ttgctccata tttatcaatc aaaattcctg caggtatttg cataaatgca
    13441 tatgcatata ggcaaaagga tgctatattt gaaatttgta tagaattaaa acctaactca
    13501 tttgctaaat tgtctgaaac aacagcagtt gacatcctta aaaataatac taaaacaaat
    13561 gaaaatgtaa ttatacccca aacactccac ttagcctttg tataactttt catatcttct
    13621 ccctcaaatc catatatttt taaattttct tgatataact tatataaagt gtatagttat
    13681 tcttacaaat ttttacacgc aatatcatta tacaacatat gtatatattt aactagaaat
    13741 aaaacactat tcatattata tacatgaatt aactacataa ataataagag ccatctttta
    13801 acattactca aaagatagct ccatataaaa acatttattc taaatttaat tttttatcta
    13861 atataaattt tgtagcgaaa aataacgcta ttgcaactac aagactacca agtattgcaa
    13921 gcataaaact aggactctga taaagatgat ataccatatt tgtatcttca tttggtaaga
    13981 tgcttccaac tgcatctcca acaattgata ttactatatt tatagctata aaagaagcaa
    14041 atgccactat gtttctatgt ttattgaatt ttggcaattg tcccattgag attgatgtat
    14101 ataataacaa aataaatgat gaatatgaaa taaacattac tactactaat aataaaatat
    14161 ctaaaaagtt ttcagaaatc catttaaaac tagtgttaaa caaatctaaa atttcaggta
    14221 gtaaaattcc agaagtacca aaaagcataa gaactccaaa agtgaatact gcaactataa
    14281 aactaagtac agcgtatata agtgctgtta tacatttaga taatataagt gaactagtgc
    14341 taacaggaag agtaaacatt aaataacctt catcccccaa aagatttttt ctaaaccttt
    14401 gaattgtaac tacaatagtt aaaactccta atgccataaa aagacttgtt aatactagta
    14461 ttcctattcc ctgtacctga agtatatttg tattcataaa tattccatta aatacagcta
    14521 ctatcaatat ggctatatat aacggtataa atattcttcc acttgctttt aactcatatt
    14581 ttaataattt acctaacatt taaattcctc cctaaataat gcatctatag attttccttt
    14641 ttcttctctt attgcttcaa cgtctccttg taacactatt tctccttttg atatgaatat
    14701 tatctcatca catatgtttt ctatttcact tattaagtgg gtagcaatca gtaaagtact
    14761 atcttcagaa taatttttta atatagtctt aagtatatat gaccttgcag ctgggtcaac
    14821 accacctata ggttcatcca atatatatat gctcgcattt cttgacatta ctaaaataag
    14881 ttgaactttt tctttagttc ctttagacat tgtctttagc ttttcattta catcaatatc
    14941 taagctttta atcatctcat ttgctcttct aacatcaaaa tcactataaa aatcatagaa
    15001 aaacttaagt aaatctgata ccttcatcca atcatttaaa taagttcttt ctggtaaata
    15061 tgaaactatt tttttagtat ctatggatgg tttcattcca tttatcataa tttcaccatt
    15121 atcaggctgt aataatccat tcattagctt aatcattgta cttttgccac ttccattagg
    15181 tcctaacagc ccgactattt ttccctttgg tatatttaaa tcaatatttt ttaaaacatt
    15241 cttagttcca taactcttgt ttacaccttt aaattcaact attccatctt gagaaacatt
    15301 gcttatattt ttatccattt tataattctc ctttcagatt ttctgtaata agttttagca
    15361 tttcatctct atcatatcca agcttttcta aggttacttt taatgtattt atctccacat
    15421 tagctatttc ctctttcata gcttttattt tttccttatc atctgttaca aatcttcctt
    15481 tagttctctg actataaact aaaccttgtc tttcaagttc tgttagagcc ttttgcattg
    15541 tatttggatt aacttccgca tcttctgcta gtgctctcac tgtttctaat ttagatccta
    15601 ttttaatttc ccctgaaatt atttttaact ttaaatgttc tactaattgt atatatatag
    15661 gtttgttatt atcaagttcc cattccatgt tcatgcctcc ttattatatt attgtattaa
    15721 gttgttaata caataatata ataatacaaa gtatatgtca attacttttt agtaaaagta
    15781 taaaaaattt tacaaaaaaa atgaacacta cattagagtc catttttaac acaatttatt
    15841 aaattcttta tattacagat taattaccct cttaccaagt tagatacaat taaatgttca
    15901 aattcttcaa ttggcattgg ctttccaaac aaatatcctt gtgccatatc acactttact
    15961 tcttttaaaa gtttaaactg ttcttctgtc tcaactcctt ctgatatgac tttcataccc
    16021 aactcttttg ccatttctat tactttagta acaattattt tgcttctttt actattataa
    16081 gtttccataa taaatttttg gtcaagtttt aagatatcca taggaatttc ttttaacata
    16141 tttaatgatg agtaccctga accaaaatca tccatagata ttaaaaatcc tatttttttt
    16201 aaattattca ttatatcaat taaaatatca aaattatcaa atatgatact ctccgttaat
    16261 tcaagttcta tatactttgc aggtatattg tatttatcaa ttatattttt gtatgtttct
    16321 ataaaattat tgcaatataa atgtatcctt gaaacattaa ctgaaacagt aactggattt
    16381 attcctgcat caatccattt tctaaggtat atacatactt cttcaagcac atataaatct
    16441 aaatttataa caaaaccgtt tctttcaaaa aatgggataa attcatttgg aggtattagc
    16501 cctttatcag gacgtttcca cctaacaagt gcttctgaac cttgtatctc tcctgtatta
    16561 agttctatct taggttgtaa gtatataata aattctttat tttctagact tgaaaacatt
    16621 gtattttcca tctctttttc ttttatctct tggtcatgaa tcttattatc ataaaatgca
    16681 tatgaacttt tatgtcctcc ctttgccatt ttatgaggta tattagccct atctatgatt
    16741 gagttgatat ctctatcttc tggaagtata aaatagattc cacaacttaa aactaactta
    16801 tatttttctt cttgattatt attaaacaag tctaattctt caaatatagt ttttaacctc
    16861 tttgttatat cttcttcttc tatatatttc ataattataa taaaattatc cgcagatatt
    16921 cttgcaaaca tttcctcttc aaatatattg ctcttaataa tctttgttaa attacgcaaa
    16981 atttcattcc caacttcata tccaaataaa tcatttatat atttaaattt atcaatatct
    17041 atatataata aagcatactt tacttgagta tttttacata gtattttgtt agcatcaatt
    17101 acaaatttat ctatagtatt tgctcctgta agattatctg tatatgctat cctttgcaat
    17161 ttaattttaa aatttaattt atcatatatt atatagaata taagtattac acatattata
    17221 gccataataa gtattatttg tattgcatgt ttgctaataa gctgattatt agacacaata
    17281 gactcaaagt tatttatgga gttgttttta tagagtttgc ataaagaata gactggtgtt
    17341 gtgtatgtaa tttgtaaaat tatacaaata aataaaaaaa acatcaattt tttttgtaaa
    17401 ttcctcataa aaatttcctc ccattaaaac acctctattt tattagcaaa taagtacatc
    17461 tcaaaagttt ccatttcttg taaaatggat agtttatatc tactttaatt ttgtaaaatt
    17521 ttctgctact ataattata
//
LOCUS       DAMJYX010000073        17308 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJYX010000073 DAMJYX010000000
VERSION     DAMJYX010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.074"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            100..2163
                     /locus_tag="PC479_002556"
     CDS             100..2163
                     /locus_tag="PC479_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2805094.1"
                     /translation="MTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSALMK
                     LFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFFKN
                     MVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYELD
                     QIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEEKG
                     VESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVEEL
                     GDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHISPH
                     LLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSIILP
                     NEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEISI
                     VPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNVLL
                     YTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKDKY
                     ISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGSTY
                     TEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMIDDS
                     GGGRK"
     gene            2160..4025
                     /locus_tag="PC479_002557"
     CDS             2160..4025
                     /locus_tag="PC479_002557"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2805095.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4149..4963
                     /locus_tag="PC479_002558"
                     /pseudo
     CDS             4149..4963
                     /locus_tag="PC479_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4988..5476
                     /locus_tag="PC479_002559"
     CDS             4988..5476
                     /locus_tag="PC479_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF2805096.1"
                     /translation="MYEKDYKQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLAN
                     RDEEKTAFSIINRYIRFVDKDDEKIRSEWKTNDQWSYFIGEGRAKLKLTTNPEPYNIQ
                     KTLNWLSRQVAPTLKTIKRLDSLNNSTLIEGLIRYARLTDKHKKIIEQNSVNVEDIIY
                     DE"
     gene            5589..5750
                     /locus_tag="PC479_002560"
     CDS             5589..5750
                     /locus_tag="PC479_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805097.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            5990..6217
                     /locus_tag="PC479_002561"
     CDS             5990..6217
                     /locus_tag="PC479_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805098.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6214..9153
                     /locus_tag="PC479_002562"
     CDS             6214..9153
                     /locus_tag="PC479_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2805099.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9153..9827
                     /locus_tag="PC479_002563"
     CDS             9153..9827
                     /locus_tag="PC479_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805100.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            9988..10404
                     /locus_tag="PC479_002564"
                     /pseudo
     CDS             9988..10404
                     /locus_tag="PC479_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10364..12811
                     /locus_tag="PC479_002565"
                     /pseudo
     CDS             10364..12811
                     /locus_tag="PC479_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12951..13373
                     /locus_tag="PC479_002566"
     CDS             12951..13373
                     /locus_tag="PC479_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805101.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13911..14945
                     /locus_tag="PC479_002567"
     CDS             13911..14945
                     /locus_tag="PC479_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2805102.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15032..15235
                     /locus_tag="PC479_002568"
     CDS             15032..15235
                     /locus_tag="PC479_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2805103.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15406..16566
                     /locus_tag="PC479_002569"
     CDS             15406..16566
                     /locus_tag="PC479_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2805104.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16825..16965
                     /locus_tag="PC479_002570"
     CDS             16825..16965
                     /locus_tag="PC479_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805105.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7088 a         1677 c         3002 g         5541 t
ORIGIN      
        1 agaaatattt tagcataaga tgtataataa aggaaaaaat acagaattaa aaaacataag
       61 atagataagt ggacttaggg gagagaaatt attatgcaat tgacaaaaat aattcttagt
      121 aactataggt gttttggaga agaacctaca actattgaaa ttaataattt aactggattt
      181 ataggtcata atagcagtgg taaaactgct ttattaagtg cacttatgaa actttttgga
      241 gaaaagtcta gtgatagaat tattgaaaga tcagatttcc atatacctga aagtattgat
      301 ccagatagta tttctaagaa tgaattatat attgaagcac aatttgagtt tcctgaattg
      361 gagagaggta aagacagcat atctatacca gtatttttta aaaatatggt tgttagtgaa
      421 ccaggtggga aaccttattt aagaattaga cttgaagcaa attgggagaa aagctcaaat
      481 cctgaaggaa gtattgattc aaatgtatac tatataacat ctacaacaga ggagataaat
      541 cctgaagata agcgtaatat caaaagatat gagcttgatc aaataaagat gatttatgta
      601 cctgccatta ggagaccaga tgaacagtta aaaaatgtct caggaacaat aatttcaaga
      661 ttattaaatg gtataagttg gtcagaacaa acaaaggcaa gaattaaaac gaaagcaagt
      721 gaaacggagg aaattttctt tgaagaaaaa ggggttgaat cattaaagaa atcactcgaa
      781 actcaatgga atagttatca taaggatatt agatattcta atccaaagat aaacttcaat
      841 agtactgatt tagagacaat tttaaaaaaa gttgaagtaa ctttttcacc tacagggaca
      901 catcgagatt atcgggttga agagcttgga gatgggttac gttctttatt ttatctttca
      961 cttgtaaata catttttaga aattgaagaa gaaattctaa aatctattaa tgatggtaaa
     1021 gaagaagata tatttacatt aataccgcct gtattaacaa ttgttgcggt tgaagaacca
     1081 gaaaatcata tttcacctca tcttttagga agggtagttg aaaaattaaa ttttatttct
     1141 acaaaagaaa attcacagac aatattgaca tcacattcac cagcaatagt aaaaagaatt
     1201 gaaccaacag acataaggta ttttaggatt tgtaaggaat atgaatgtac aaaagtgaag
     1261 tcaattatat taccaaatga agaaaaggaa gcagaacaat ttaaatttgt aaaggaatca
     1321 gtattagcat atcctgaact ttattttgca aaacttgttg tattgggcga aggtgatagt
     1381 gaagagatca ttattaagca gatgattgaa ttaaaagcag aatctgtaga ttcatcagaa
     1441 atttcaatag taccattagg tggtaggcat gttaatcatt tttggcgttt attaaatgat
     1501 ttagaaattc catttattac tttgttagat ttagatagag aacgagatgg aggtggttgg
     1561 ggacgtataa agtatgcaat acaacaatta ataaatattg gtcatgataa gaatgtatta
     1621 ctttatacat gtgaaggaat ttcacaagat aatgaattta aagagatgca tacttgggat
     1681 gtaactgata ctgaaaatat ggctaaatgg attgaaatgc taaaaggtta tgatgtttat
     1741 ttttcagctc cgttagatat agattttgca atgttacaaa gttttaaaga taaatatatt
     1801 tcagcattaa caagtgcgga aggtcctagg ataaaagatc atggaaaaat acaagatatt
     1861 gtgatcaatg aagatgctga agatgaatca ttgaaagctg cttataaaga tagaatagat
     1921 agtgatatta aatcaacctt aaaacaagaa ggtggtgatg gatctactta tactgaggga
     1981 gagaaagaac taatgatatg gtattcatat ttctttttag gaagaggtaa accaactact
     2041 catttatctt tattttctaa taatactgat atagattttg aaagttttcc atactgtcta
     2101 aaggagtttg taaatagtat tattgaaaag atgatagatg attcaggggg tggaagaaaa
     2161 tgaagaggaa ttgggttagt aaagaagatt ggagtccagc ggctggattc agactagaag
     2221 ttgaagcgat gaatacagtt attaatgata agaatacact tgttgtagct ggtcctggcg
     2281 ctggaaaaac agagttatta gctcagagag cctgcttttt actagagact aatacctgta
     2341 gatatcctaa aaaaatcctt gctattagtt ttaaaaagga tgctgctgaa aatttaaaag
     2401 agcgtttaga attgagatgt gggaaagaat tagcttcaag atttgaatca aagacctatg
     2461 atgcttttgc aaaagaaatc gtagatagat ttagaaatag tttagatgaa gcatatagac
     2521 ctttaaataa ttacaatata gctgaaaatc gtgatgttag aagagcattt gagatagcag
     2581 gattaaattt cagaggaaat caaactgaat ttaataaagt atatggaaat aaactatcga
     2641 taaataaatt gcctttacat acagattttg tagataagat ttttatagat gcttggaatg
     2701 tactacttaa aggcaacaaa gatactaatt ttgtaagttc acttactttt ggtatgatat
     2761 cacgtttagc cgaatatttg ataagaacca ataggtatat taagaaagca attgaaatga
     2821 cttatagtca tgtgttttta gatgagtttc aagatactaa atctattcaa tatgatcttg
     2881 ttaaagcttg tttttttaaa acagaaacaa taattactgc tgtaggggat ggcaaacaac
     2941 gaattatgtt atgggctggt gctagaaagt caatatttga agattttcaa aatgattttt
     3001 taagtcaaaa aagaacatta attatgaatc atcgttcagc gccaagactt gtagagattc
     3061 aaaaagcaat gtataacagt ttgaatgaag aggcaattga tttaaaaact aatgaaaagt
     3121 ggaattatga agatggagaa gtgtatttaa gactttttga ggattatata aaagaagctg
     3181 atatacttag tgaggaaata gtcgaattgg tggaaacagg tatagaaatt aatgaaatat
     3241 gtatattagt aaaacagagc gtagatattt attcaaaaga gattataaat aaactttctg
     3301 aacgtaatat taaggcgcgt aatgaagcaa tgtatcagga gatattaaaa gaagatataa
     3361 taaagacact ggtttcgctt tttaggttgt cgttaagtga tagaaatccc gatgattggg
     3421 attatattta cgatattcta ggtgaattat atggaataga cgaagggtat aaccctaaaa
     3481 tattaaatga tttcattacc aaagtagaat taatgattaa tgatctcaag gtaaatttaa
     3541 atattactga tgaaaaacaa ttttcagatt tagttgattc taaggttgaa tatatttcat
     3601 atgaaaaatt tgcatcaaag tttcaaaaat ataaatctaa agattatttt gatgatttgg
     3661 tagataaatt taaaaagctg gtatggattg aatataatga aactaaagat tggattaaag
     3721 cgattgataa ctttgaaggt aaaaattcaa ttccaataat gacaattcat aaaagtaaag
     3781 gacttgaata tgatactatc ttttttgttg ggttagaaga ttcagcattt tggaatttta
     3841 aaaatcaacc agatgaagat agatgtgcat tttttgttgc attatcaaga gctaaaagaa
     3901 gaattgattt tacatttagc ttagatagac cagcaggatt ttcgaattca cagaagcatt
     3961 attgtattaa tgaattctac gaattgtttg ataatactga gattgtaaaa aagatagagt
     4021 attgattaag atgataaaaa ctttagtata ttatatttta agatattagt tatataagta
     4081 gacattgaat ttatgtattt gatgtctatt ttttatataa aaatatatta aagaaaggat
     4141 gaatttgcat gaagaaaaaa acaaaaatat ctaaaaagat tgtaagtttt attatggttt
     4201 ttataactat agttactact atacttacat catcagtttc agcttatgct agcgagttgg
     4261 atatgagtga acagacaggc tttaattatt taggtattag tccaattaca ggaaaaaaat
     4321 taatcataac atttatcgta tgaagatgga taataaaatt gtattttgtg tagagccagg
     4381 aattaaaact tcaagtggag gaggatatat atcggaggag ttaggagata aatttatatc
     4441 tacaacactt ccaaattatc ataaaagaaa agctgaaata cttgagaagg taaatagaca
     4501 taatatatta ccttcatgga ataatgaaaa agttgcagtt aaaagtggag aaacaattga
     4561 gttagaagat aagaatggta ttattagaga aatgcaatta gaatcaaata atactggaac
     4621 agatatatta cttgaagaaa atactttgaa aattacagca aataaaaatt ctaattcagg
     4681 agttattagt tatataaaga ttcctaaaaa tgaggttgga atatcaatag tttatcgtaa
     4741 gcataactat caaagcttat cagaatttca tatggaaagt tcgatgagtg caaatttaaa
     4801 tataaatgta atcaagttag gaattgttaa agtaaaaaag attgatgaag gcacaggaaa
     4861 ggaattagca aatgcaaaac ttagatttga gtatgatggt gatagtaaag aaattataac
     4921 gggttctaat gaaattgcta gtatagaaga tattattggt tgattaaaaa gtgaagtata
     4981 cttttgtatc tatgaaaaag actataagca atatataaaa ttagatatac ctattgatga
     5041 agcaaaaata aaaaatagat ttgaaataag gttaaaaaat gaaagagcat atcatgcagc
     5101 agttgatttg ttagcaaata gagatgaaga aaagacagca ttttcaatta ttaatcgtta
     5161 tatacgtttt gttgataaag atgatgaaaa aattagaagt gaatggaaaa ctaatgatca
     5221 gtggagttat tttattggtg aaggtagagc gaagttaaaa ctaacaacaa atccagaacc
     5281 atataatatt caaaaaactt taaattggtt atcaagacaa gttgcaccaa cgttaaaaac
     5341 aattaaaagg ttagatagtt tgaacaactc aacattaatt gagggcttaa taaggtatgc
     5401 aagactaaca gataaacata aaaaaataat tgaacaaaat tcagtaaatg ttgaagacat
     5461 tatttatgat gaataaaaaa atgtaattag tattgcagat tttaatataa aagaagggag
     5521 atatacgaat gaattttggt caaaatttat ataattagtt tttatcaaat gcacaatcat
     5581 tggtattaat ggctatagct ataattggtg tatatttagg atttaagaga gagttttcaa
     5641 aacttatagg atttttagta gttgcattaa ttgcagtagg gcttgtgttt aatacagcag
     5701 gagttaaaga tatattattg gagctattta ataaaataat aggtgtgtaa ggattaaaag
     5761 agaatagaaa attatgagta tatgatgaag ggttctaata agttttatta aaatatatat
     5821 tgaaagacaa gtagagctat ttcgagttat tacatgtaat gctaatatat ataactgcat
     5881 tttggaaatt aaatacataa ttagaaatag caaattttat aggtgactta ttaatatgtt
     5941 tacttattgt atactatttg atatatttaa tatgaggtga ttcttgaaaa tggacatagt
     6001 gaaagttttt gctaacaatg tgagaaaata tagaaataaa aaaggtctat cacaagaagc
     6061 ttttgctgaa aaagctggat tgcatagaac ttatatttgt gctattgaaa gaaaaaagag
     6121 aagtattgct ttagataatg tgcagaaaat tgctgatgcg cttgagattg agacttactt
     6181 actatttata gaaggtgagg agaaagaatg tctatgaaaa aattaccaat agaaaagctt
     6241 atagaaaatt taggtttttc agggtcaaag aatcttgtga atatagatat gcttacaaaa
     6301 ttagatatat ctaaacatga tgtaaaaact ttaaaagtta taaatcctta tgcaatttat
     6361 ttagtaaata gtaagccatt tatattattt atagagtctt cttttgaaga aaaaaaatta
     6421 caacaaatta gtaagcttat atggaatgct caaataccaa ttgcttttat atgcgattct
     6481 aattctgtta aaatatttaa tggaaaagct ttggatttaa agtcatattt aattgaggaa
     6541 atatataatc aatctattga ggatttttcg gaagaatctg agttttctta tttgaaaata
     6601 tctgatccat tattttggga tcaatacact aaacaatata ccaaaacgca tttaaatgat
     6661 tatttgctag ataatattac ttcattgaca aataaattaa agaaaaaata taaggtgaaa
     6721 tttgcaacga agcttgttct aagattaata tttattagat atttaatcga tagaggtgtc
     6781 gatatagctt ataaaaattt taatcatgat attaaagctt cacaaaaaga actgctaaag
     6841 attactaaaa acaaagttga attatatgaa ctgtttaaat acttaaaaga aaagtttaat
     6901 ggtaatttat ttgagttaaa tgacgaaatt aataatactt gtttaacaga tgaagtgttt
     6961 attttactat ctgatttttt atcaggtaaa ataagtattg acaatggaca aatgtcttta
     7021 ttttcaatgt atgactttaa tatcatacca gttgaactaa ttagtaatat atacgaaatt
     7081 cttttaggaa aagaaattcg taataaagat aacgcattct atactccgaa ttacttagca
     7141 gaatatattt tagataaaac aacacttgaa tttctgaaaa ataataggca atacaaaatt
     7201 ctagatcctt cttgtggatc aggagtattt ttagtaaata gttttagacg tattattgat
     7261 atgaatttgg gtgataaact tttttgtgaa gatgataaac ttttaaaaga acttttatta
     7321 tctaatattt atggaattga tatcaatgaa gatgctatcg atgttactat tttctcgtta
     7381 tatctaacta ttttagatta caaagaccct aagatattat cagaatttaa actgcctaat
     7441 ttaaaagggt gtaatttaat agcgagtgat ttttttgatg ataataaatt ggagagttta
     7501 aagaaaatta actttgattt tatcattgga aatccgcctt ggggaagtgt ctcagatgga
     7561 ttacatttag attattgcaa taaaaatggc tataatgata tgcaacaaaa caaagaaatc
     7621 agtagaagtt ttatttttag agcaaaagat tttagctcta gaaatacagt atgttctttt
     7681 gtagtgcatt ctaaaatact atatactcaa aaaaagccat caaaaaaatt ccgtgaattt
     7741 ttgcttaaaa aaagtcaaat atttagtgta gttgaaatgt catctgtaag aaaattagtg
     7801 tttaaaaatg cagacgctcc tgcagcagtt atatcattta agtataacga aagtgaagaa
     7861 aatttaaata atagaatcat ctatacatct ttaaaaccaa atgtattttt taaattattt
     7921 aacattattg taattgaaaa atatgatgtt aaatatattt cacaaaaact attattggag
     7981 aatgattggg cttggaaaac tattgtgtat ggattcagta aagatataga tataattatg
     8041 aatatcaaaa aaaaatatca aactgtaaat gaaattattt tgaaaaacag ttttgttcat
     8101 ggaacaggga tacaaatcaa tggtaatgaa agccatgatg cgacacattt aaaaggaaag
     8161 tggtatgttg attcaaagaa aggaatagat tcattctatg ttgatataaa taaagggaaa
     8221 gaacttactc aagataaaat gagaagacct aaggctaatc agcaagaaat atttattcct
     8281 ccatatgtat tattaaaaaa gggagttgac acgaaaacat atagatatag agcagcattt
     8341 tcaaatgatg catttgtata ttcagatgca gttacaggaa tatgtggaaa aaaagataat
     8401 ttaactacac tttattgctt gactggtatg cttaactctt ctttttactc atacttaaat
     8461 ataatgctag gtacatcaat tggaatagaa agagaacaag gattttcaac ggaaattttt
     8521 acgtatccag caatagaaag tgatgaaatt gctaatctgg tagtaaaaat acaagaagcg
     8581 actaaattag aaaaaacaac tatgttcttg aatgaaaaaa atagttgtaa gtatatcaaa
     8641 caattagata agttaatttt gaagtctttt aatttaaatg atgataaatt tattgactat
     8701 gcaataaata ttcaaattcc tgagcttaca aattctaagt caactaacat atatagaaaa
     8761 ataactaagg aagatttaat taattatagt gaatgtttca aagaacagtt ttcatcgata
     8821 tatcgtaggg ttgggaaaag tatttcaatt tcggtatatc caaaaatatt aaatcgtttt
     8881 acagtatttg aactagaagt aacagatgaa aaaacagata gagagattat ggttaaagca
     8941 gaagctgata ataatatgga gttgtttacg aaaataatta gatatgatta taatgatgtt
     9001 tttcatcaaa ttagagatat tattcatttt ggagaaaatt cattttatat cataaagcct
     9061 aattgctata aatattggca tcctgctatc gcagaaatgg atcttgctga tgttttggat
     9121 cagattttat ctgatacagg gggtgaagaa taatgggagg atttaaaaat tcttcactaa
     9181 acttttattt aaagcaaggg ctttatgaaa taattataga tggattaata gactgttgtt
     9241 tacttatgag caaatcatgt attgaacaat ctagaaaaat attaaatcac gaagaaaaga
     9301 taagaacgca tttagttgaa aattatttag aaaatgatat tgaaagaaga aaaacatctt
     9361 tatctaaaca acatctaaga tttgaagtag aagcacaaga aaattatgac aacaaaaatg
     9421 aatcttatga gggtagggtt gatataaaag ttatatctaa agacacttta gcggatagaa
     9481 gggcttatta tatcattgaa tgtaaaagga tagatggtaa ggaaacttta aataaaaaat
     9541 atataaccga aggtgtacaa cgatttatta ctgagaaata ttcttcgcat tatggcaaaa
     9601 atataatgct tggatttata gtaaaagata taaatatcca gagcaatata caaaaaatta
     9661 attcactgaa tcagaacata ttatatacat atatagatac agatatgttg acagttagaa
     9721 gtgattctga aaataattat gctgtttgtg aaagtaaata taaagtaaaa tctaaactac
     9781 tattgttaaa tcatattttt tataactttt cgagtataat aagataatct tatgaatata
     9841 aaatattact atgttaaaat tatagtttgg ttttgataat attataaaaa taagtttaag
     9901 aataaaatat aaaggaaaaa aggggcgtag cactattttt tagtgtgaca gcccctttaa
     9961 ttttaaaatc ggtaaggagg agtgtatttg aaaaaaatta agtcatatac agggattttg
    10021 aatgtggaga aggttatata tgcaataaat gattttcagc ttccatttcc agttactttt
    10081 acgcagatga catggtttgt attaacactt tttataatta tattatttgg taagttacct
    10141 ccattattta tgatagaagg agcattttta aagtatcttg gcataccaat ttttataact
    10201 tagtttatgt ctcaaaagac ttttgatgga aagaaaacat atggattttt aaagtcaata
    10261 atactatatt cattaagacc taaaataaca tacgcaggaa aaaacattaa atttaaaaat
    10321 aaaattattg atgaaaatat aactatagta aggagtgaat tatatgttcc cgataaaata
    10381 tatagagaac aatcttgtat ttaataagga aggagaatgt tttgcatatt atgaattaat
    10441 accatataat tattcattct tataagctga acaaaaattt attgtacatg ataattttag
    10501 gcaattaata tcacaaagta gaaatggtaa aattcatgca cttcaaatag ccactgaaag
    10561 ttctataaga gttacacaag aaaggttaaa gaaaaatatt acaggaaaat taaaagaaat
    10621 tgcttatgaa aaaatagacg agcaaacaga ggcacttgtt tctatgattg gcgataatca
    10681 aattgattat cgttttttta ttgggtttaa gcttttagtt acagagaaag aaatgaatat
    10741 aaagagtata aaaaagtcaa tattagctaa tctatcagat tttatatatg atgttaataa
    10801 taaattgatg gatgactttg tcagtataaa taatgatgaa attatgagat ttttaaaaat
    10861 ggaaaagtta ttagaaaata aaatctcaag aagatttaaa gttagaaggt tagataaaaa
    10921 tgattttgga tatttaattg aacataatta tggaaagaaa ggcacagttt ttgaagatta
    10981 tcaataccat ttacctacaa agaaacttaa aagagaaaca ttagtaaaga aatacaatct
    11041 tataaagcca actagatgtc ttatagaaga gaagcaaaga tacttaaaaa ttatacatga
    11101 ggattcagaa agttttgtag cttattttac tattgaaatt attattggag aaatgaattt
    11161 tccatctagt gagatattct attatcagca acaacagttt gcatttccta ttgatactag
    11221 tatgaatgtg gaaattgtaa caaataaaaa ggctttaact acagttagaa ataagaaaaa
    11281 agaattaaaa gatttagaca atcatgcttg gcagtcaggg aatgaaacag taacaaatgt
    11341 agtagaagca ttagaggctg taaatgagct tgtaaataat ttaaatcaaa caaaagaatc
    11401 aatgtataag ttaagctatg taattagggt atcagcagat agcattgatg aattaaaacg
    11461 ccgttgtgat gaagttaaag atttttatga cgatttaaat ataaaattag tacgcctaat
    11521 gggagatatg ataggatttc acaatgaatt tataccttca agcaaaaggt atataaatga
    11581 ttatattcaa tatgtaacta gtgattttct agcaggatta gggtttggag caactcaata
    11641 gcttggagag aacgaaggaa tatatattgg atatagttta gatacaggaa gaaatgtcta
    11701 tcttcaacca tctcttgcaa gtcaaggagt aaaagaatct gtaactaatg cgttggtagc
    11761 agcattcttt ggatttttag gaggtgggaa atcatttgct aataacttac ttgtttacta
    11821 ttcagtattg tttggagggc aagctgtttt ggtggatcca aaagctgaac gtggcaaatg
    11881 gaaagaaaat ttaccagata tatatcatga agtaaatatt gttaatttaa caagtgatat
    11941 agaaaataag ggaatgctag atccttatgt aattatgaaa gaagtaaaag atagtggaag
    12001 tctagctatt gatatattaa cttttcttac aggaataagt agtcatgatg gagagcagtt
    12061 tcctatactt agaaaatcaa tcataaatgt tactcaagac aaatgcagag gacttttgaa
    12121 ggtaatagat gaattaagga aagataattc tcaaatatca aataatattg cagatcatat
    12181 tgaaagtttt acagattatg attttgcaca attacttttc tcagatggaa atattaaagg
    12241 ctcaattaat ctagaaaaac aaatgaatat aatacaagtt gcagatcttg ttttaccgaa
    12301 taaagatact ttatttgaag aatatacaac aatggaaagt ctttcagtag ctatgttgat
    12361 agtaattagt acttttgcat tagattttat ccatacagat agaagtactt ttaaaatagt
    12421 agatttagat gaagcttgga catttcttaa tgtagcacaa ggaaaaactt tatctaataa
    12481 attagttaga gcaggatgtg ctatgaatgc aggagtgtat tttgtaactc aaaatgcagg
    12541 tgatgtatca gatgaaaatt taaaaaacaa tattggatta aaatttgcat tcagaagcac
    12601 agatatcaat gaaattaaga gaatacttga attttttgga gttgataagg aagatgagaa
    12661 taatcaaaaa aggttaagag agttagagaa tggacagtgt ttaatacaag atttatatgg
    12721 acgagttggg gtaatacaaa tacatctagt ttttcaagaa ctccttactg catttgatac
    12781 aagaccacct ataagaaaag aggtggatta atgaaatcga aagtatttag aaatttaagc
    12841 tggataacta ttttaatagt agtactttta ctatttttta tttatataaa tttatagatt
    12901 tagagaaaca aatagataca ttagataaga aaagaaaaaa actcatagat atgagattag
    12961 aagatataat tgataaagaa tcatatgaag aaaagtatga tgatttaaca gttgaaataa
    13021 agaaacttag taaagagaaa aaaactctta aagcatcatt aataaatgaa aagagtataa
    13081 aagcaagact tagagaattt agaaagatac ttgaaagaaa tgaattgtta gaaaaatttg
    13141 atagatatgt atttgcaagt atagtagata aggttatagt tggagttatt aatgaagatg
    13201 gtatagttga tccttataaa ataacttttg tttataaaac tagctttgat gataaacaag
    13261 atggacataa atttataaat aagagtccgt tagaaacgta ttcaaaagat aacaacgata
    13321 ataaaaagaa gtgttcatat actaataatg agacattgcg agactgtagt taaacttatt
    13381 agagaataac aaggtttcag acttttatat tgtgtcttaa aagtagattt gccacctatt
    13441 tgccaccata attatttttg ggtggcaaat ttattctaca atgatttcaa aaatatttac
    13501 cgtctggttt ctcatgttgt tagttacatg taaatatgta tccatagttg ttgaagtttt
    13561 agcatgtccg agtatatgct ggatatcttt tatattagta ccattttcta ataacatagt
    13621 atcagggttt ttaaaataaa gtgtgtattc ttctaaagta gtgataatta aaatatcaaa
    13681 tatcactata caaaggaaag tgcacgcttt ttatgtttaa caaaaacgaa atattaaaaa
    13741 acttattgaa gaatatgatg tcaaaacaac tacagatatt caagatatgc taaaagattt
    13801 atttacaaat acaattcatc aaatatttac attgtcgaat tttagcggtt taaacataat
    13861 atattgactg atataataaa atcatagaat attttagggg gaaatagatt atgtttaaga
    13921 aaaagttaat agctgttgca atgagtataa ctatgattag tgtagggagc ttttcgtatg
    13981 ctcattcagg aagaactgat tcatcaggag gtcatcgtga taataaaaat aaatctggat
    14041 taggaagtta tcactatcat tgtggtggtt atccagctca tttacataat aatggtggtt
    14101 gtccttatac tggaggagga agcagttctg gaacaacaac ttcggttaat aatgaagaaa
    14161 agcaaaagag gtctgttgga gaaaaaggat ataatcaagg gtatgaagat gggtataaag
    14221 gaaattatag ttcatcaaat tatagtggag attatagtga tacatatgaa tcaaagtact
    14281 cagaaggata tgaaaaaggt aaggctaaat tagaagagga agaaaaggtg gctaaagaga
    14341 ctggatataa tttagggctt acaggtgcta aatctaataa tacatatgaa aaagaagttt
    14401 taaaaaatgc ttatgatacc ggatattcaa ctggatacaa tgaatataag actaaaaaga
    14461 tagaagaata taaagctaaa gggatagagg atagcaataa agataaggaa aaaatgacat
    14521 ttgaagaaaa catagattct gaatttatag atgcttataa taatgcttat gacgaaatac
    14581 aagaacaatt aaaaaatgat tacactactc aaggatttga gagtgctatc aaaggagaaa
    14641 gatttgacac atcaactata ggtaatgtta aatatgctaa ttggtttaaa gaaggatatg
    14701 ataatggtaa ggttaagtta cctaaagcaa aagaaagtgt atataatcaa ggttacaatg
    14761 aagaagattt tagtatacca gatgaaatga agtctattga aactaagtta aaaggtgtat
    14821 atgatgaagg tttagaaaaa agagaagaag agaaaagtag aaatgctact tatggggttg
    14881 gagctggaac agcagctgta gttgctggag tagtatataa gaagaaaaaa cgtaaaaaga
    14941 tatagatgct agatatacca atgatgacaa gtgtataaca aatataattt taagtataaa
    15001 tatttaaaat atagaaggga aggttatatc tatgggattt aactttagga aaagcataaa
    15061 tctaggagga ggactcaaat taaatttagg taagaagagt gtaggtataa gtgctggtgt
    15121 taaaggtgct agagtgtcag ttaattcaaa aggaagaaaa tcagctacat tgagtatacc
    15181 tggaactgga atatcatata caaaaactag tacaaagaaa cgtaaaccta aataaatatt
    15241 tgatatacta agaaatagac tgttacaaat agtctatttt ttagtatata gatttaaaaa
    15301 gtgacaaaat tttgaaatat aaactaattt cataaaggaa aatattattt tatgtagaat
    15361 tataatattt aagtaatatt ataaaattta taggaggatt tcattatgaa attaagcaaa
    15421 atactattgg caatagggat gtctgtttca ttattgattg ctagttgtcc aacatcaaac
    15481 gctcttagtt ctattaattc aataaaaggt acaaataagt atgaaacagc aggtttaata
    15541 gcagataaac aaaattatga tattgcaata cttattaatt ctgaaaatgg attatctgat
    15601 ggattgtcag caagtggatt gtcaggagct aaaaatgccc caatattgtt aactaagaaa
    15661 gatacaatcc cttcagaaac attaaaaaga ttagaaaagg taaaagaagt atatctaatt
    15721 ggaggaaaaa attcgatagg agcatcagtt cagaaaatat taagtgataa aaaaataagc
    15781 ataaatagaa taagtggaac tgatagaata gatacaagtc ataaagttgc aaaagaaata
    15841 ggaagagtaa gtacggttaa aaaagtaata ttaactaatg cttttaaagg cgaaccagat
    15901 gctatgagtg ttgcttctat agcagttaga gataaatctc caatagtatt aactgatgga
    15961 aatactacaa gttttgctac agatggatta gaaacttatg caattggtgg aaaagtcagt
    16021 atgagtgata agttaataaa tgaaactaaa gcaacaagaa taggtggagt agatagatac
    16081 gatacaaata aacaaataat aaataaattt tataatggct caaaagaatt ttacatagca
    16141 agtgggactg atttagttta ttctttagtt ggttctacaa ttgcacaaaa aacacctata
    16201 gtgttagttg gaaatggaag taataagagt atattaaaaa atgcaagtaa aataactgct
    16261 attggaaata ttgatgatgt tgttataaaa caatgtttaa atataactaa taatgttggg
    16321 gattcaaata caggtattgt aaaagatact aattcaaata atacgaacaa accaagtaat
    16381 gagcctagct ctaataatcc tggattaact gataaaagta cagtatatgt aactcctaat
    16441 ggaaaatcat atcatctaac aaaaaattgt tctacactaa aaagaagtaa aacaataaat
    16501 aaagtaacat taaaacaagc taagtcacaa ggtaaaatag atccttgtaa tgtttgtgta
    16561 aaataatgta ttagccaaat atatattaaa aaagacaatt aattaagatt gtctttttta
    16621 ttgaagaaat ataaatttat attatgtatt attaaaaaaa aattattaat tataaaaaat
    16681 aataataaag acgataagta ttaaaatttt atgtttccaa tatttatgat ataattaata
    16741 tggcaaggaa taaatatttg aaaaagctat gagtggtgtt tccatggaag tttcttcact
    16801 ttcttatgag aggaggtgaa aactatggaa aatctaatga tgagtattat aactggtgtt
    16861 atagccagtt acatttacga taaattaaag agccactctg agcgaccaac taagagtggc
    16921 tgggaactta atataaagtt ccagaaaagt aaatcttcaa aataatttgt tgaaggaaac
    16981 atcactcaaa gttaagtaaa atatgtttcc ttgcttttat tatatcacaa attaaaaata
    17041 ttaacacaaa attttgtgaa tatatactta ttattgttct aaaaaaatat gtaaataatt
    17101 gatttgattg catggaatta ttgatgtaat atttaagtta aatatacaca atctcaatta
    17161 aagaagataa gtactataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
    17221 tataaaaagt cttttagtat cattatttca agaggaatta tcatatgaaa aaataacttc
    17281 attagaaaag tggataaaaa actggtaa
//
LOCUS       DAMJYX010000074        16681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJYX010000074 DAMJYX010000000
VERSION     DAMJYX010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.075"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            185..1588
                     /locus_tag="PC479_002571"
     CDS             185..1588
                     /locus_tag="PC479_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2805106.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2065..3087)
                     /locus_tag="PC479_002572"
     CDS             complement(2065..3087)
                     /locus_tag="PC479_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2805107.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3122..4720)
                     /locus_tag="PC479_002573"
     CDS             complement(3122..4720)
                     /locus_tag="PC479_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2805108.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4762..6027)
                     /locus_tag="PC479_002574"
     CDS             complement(4762..6027)
                     /locus_tag="PC479_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2805109.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6169..7596)
                     /locus_tag="PC479_002575"
     CDS             complement(6169..7596)
                     /locus_tag="PC479_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2805110.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7634..8641)
                     /locus_tag="PC479_002576"
     CDS             complement(7634..8641)
                     /locus_tag="PC479_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2805111.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(8838..10160)
                     /locus_tag="PC479_002577"
     CDS             complement(8838..10160)
                     /locus_tag="PC479_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805112.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10518..12158)
                     /locus_tag="PC479_002578"
     CDS             complement(10518..12158)
                     /locus_tag="PC479_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2805113.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12314..12970)
                     /locus_tag="PC479_002579"
     CDS             complement(12314..12970)
                     /locus_tag="PC479_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805114.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(12996..13697)
                     /locus_tag="PC479_002580"
     CDS             complement(12996..13697)
                     /locus_tag="PC479_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2805115.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
     gene            14505..14984
                     /locus_tag="PC479_002581"
     CDS             14505..14984
                     /locus_tag="PC479_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2805116.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            14981..15760
                     /locus_tag="PC479_002582"
     CDS             14981..15760
                     /locus_tag="PC479_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805117.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(16010..16510)
                     /locus_tag="PC479_002583"
     CDS             complement(16010..16510)
                     /locus_tag="PC479_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805118.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT         5803 a         2996 c         1986 g         5896 t
ORIGIN      
        1 aatactttat tcaatacata agtagaaatc tctatataca taaattcatt ttttcataca
       61 tcttacaaat ttatttttat ttagataaca tgtatatttt ttccatgaaa aataaataat
      121 aaaaaatagt aaatatggaa aatataaaat tgattaacaa taattaatta cctgaggtgt
      181 gaatatgaaa atgctaaaca acttttttaa gaaaatcaac aatccagctc ctgtctttgc
      241 acagacaaaa atggaaagag ataatcaaac agatgattca ctttctacaa aaccaaagac
      301 tacatttaga gacgttgctg gattagacga agtaaaagaa gaactatttg aaatagtcga
      361 ttttatgaag tcaccacaaa aatatcaaaa aatgggtgca aaaataccta aaggagtatt
      421 attttatggt cctccaggaa caggtaaaac cctacttgca tctgctgtag ctggtgaaac
      481 aaactcatca tttttcaatg ttactggttc agagttcgtt gaaaaatatg taggtgtggg
      541 agcaaaaaga gttagaactc tatttgaaaa agctagaaaa gaagctccaa gtataatatt
      601 tatagacgaa atagatgctg taggagcaaa gagacatctt gaaagtaaca acgaaaaaga
      661 ccaaacacta aaccaacttt tagttgaaat ggatggattt aataaagatt ccaatgtatt
      721 aataataggt gctaccaata gacttgactt attagatgaa gctttactta gaccaggaag
      781 atttgacaga cacattcata taggtgcacc aaactatcac actagatttg aaattctaaa
      841 ggtacataca gatgacaaac ctatagacaa atctgtaaat ctagaacttt tagctaaaaa
      901 aactcatggt ttcaatgggg ctcatctttc aaatatagca aatgaagcag ctatatttgc
      961 agttagagat gacagtgagt gtattacatc agaacatttt gacaaggcac ttgaaagagt
     1021 tattgcaggg ctagaatcta aaaactctgc attggttgaa aaagagaaaa aaatagtagc
     1081 atatcatgaa gctggacatg ctcttgtaag tgatatagta ggaatttgtc ctatacaaaa
     1141 aatatctata gttcctagag gacaagcatt aggttatgtt cttcaattac ctgatgaaga
     1201 tagatatatt tatactaagg atgagttgat tggaaaaata aaaatattac ttgctggaaa
     1261 ggcttcagaa gaattaatat ttaatcataa atctactggt gcaaaggacg acttaaagaa
     1321 agtcactgaa atagctaacc aaatggtttg tgaatatggt atgagtaact taggatttat
     1381 gactattgat ggaaatgata agactttctt atgtgataag gttcaaaagg aagcaaatcg
     1441 tatagttgaa atttgttata aagagacttt agagatgtta aaggataatc ttgaagattt
     1501 acattctgtt tctaaattct tatttgaaaa agaaactatg actcatgaag agttaaaaga
     1561 tttgatagga aaagaagctg taaactaata tagttttaaa aatttgaggc tgtctcaaaa
     1621 tagagataag cctcttttta ttgttaaagt tataaaaata catttgattt tatacaaaaa
     1681 agtgcctcat aaactaaaaa tttcattttg agacacttac atattttaat tacatatttt
     1741 aattttgtct atttacagaa tattatatct acataaaact aaactgctgt aaacttaact
     1801 acctccagta atgtttgaag tggtataaaa gctagtagcc tagtttctac acttataatt
     1861 tatattttac ataactaaat ttagtagatt tttatactca caatttagaa cgattgaaaa
     1921 cttaaaaatt tcttatcaga tatctacaac ttaattaggt atctatcact tgattaaata
     1981 tctatcactt aattaggtat ctatcacttg attaaatatc tgccatttaa ttaaatatct
     2041 accacttaat taaatatcta ccacttaaag tgtagaacct ctttcaatca attcaggttt
     2101 taacatttca acgactgact ctggattttc ttcatttaat aaggacttaa gtatgttaat
     2161 tgttttttca atcaacaaat ctagtggctg tctaatcgtc gttaaattat aactcttcca
     2221 tgaagccatt tgaatatcat catatcctat cactgataaa tcctctggca ccctcaactt
     2281 gtactcaaac cttgcagcat ccattacacc aatagctatc aagtcacttg tgcaaaaaat
     2341 tgctgttgga ggatttgata atgctaacat tctcttacct gcttcatatc cagaatcata
     2401 atcatagttt gcagattctt ctttaaggtt atgaatccca ctttgtctta acttagagta
     2461 aaatccaatt ttcttttcca tttcttcact tgtttctctc gtaaattgaa tatatcctat
     2521 attcttatga cctttcttaa gcagatattc agcagccatt cccccacctt caattgggtc
     2581 tacatagact gtacttattt ttataccagg tataaaacga ttaaaaagaa tcacaggaat
     2641 attgttttct tcacaagtct cagccatcac cttattcatg gccgaagctg taataatcac
     2701 agcttctact tgaaactgaa ttacttttga aattatacta tctatacttt gttgtctagg
     2761 aactttaaaa acgagacact gtttacctat ctcttgaatt ttttctacaa ataaattgat
     2821 aattctattg taaaatggac ctagactatc accaactatc aatcctataa cattagtttt
     2881 tccactaatc attccccgtg ctaccaaatt aggaacatat cctatttcat ttgctacacg
     2941 aaaaattctt tctcttactt caggttttac gccctttcca tttgcactac caaatgctct
     3001 tgaaacagat gattgagata ctcctgccaa tcttgcaaca tcttttgagg tagccgattt
     3061 attataatga actttctttt gattcattta gtatttctcc ttaaatatct agtatttctt
     3121 cttaaaattg tatcaaaaat aatataatat attggataat aaaaaaagct aatacaatta
     3181 attcttgttt acctaaagaa aatggcacta tagatactcc atctacttta tccattcctt
     3241 gagaaataag gtgatttctc ataccaaatg cagtgtattt tttaatatca tctgcattat
     3301 gatttttctt taaaatgaca ctaaaaatat atccaaatat caatgttatt gtagcaccta
     3361 ttgctggatt ccacaaccaa ctcacattat atttattaga aataaaaaat cctaaaacta
     3421 accctacaat aaaaccaata gatactcctt tatcattagc tttatatgta aacattgcta
     3481 gtacaaatgc acctgtagct ggtccagcaa agtatgagat atagttacca acaaggtcaa
     3541 gaattgattt gatgcttcct ccaaatccca taataataaa acctgtcata acaactccta
     3601 taactacagt tataatcatt gtaactttta atgatgattc tttgttatct tttttgaaat
     3661 aaaactcata aatatccttc gtaaaaacag ttgtcatact gtttaatata gagtcgatac
     3721 tagacattgc tgctgcaaac acacctgcaa taataattcc aatggctcct actggaagtt
     3781 catttaaaat aaaagtaatc attatttcat ttgaagtttc aaatgtcctt cccccataaa
     3841 aaacaaataa aattactcct accaatagca taaagtagta aactaaattc ataacaaatg
     3901 cactaactac aaaagaattt tttgcacatt ttaaagattt agatgttaaa actctttgta
     3961 cttgagattg gtcaaaacaa aagtatctta tccacattac agttccacca attaaagtag
     4021 cccaaaaagt atttgtcact gaaatatccc tactgaaatc aaatgtattt aattttccac
     4081 tctgttgtgc aatttgtaat gtatcaaaga aacttagatt cattcctttt aaggctacaa
     4141 taattactat aaatacagca cttattacta ctatcatttg aatactatca gtccatataa
     4201 ctgccttaat cccacctaat agagtgtata taattgatac aagtacaact attgggacta
     4261 aagcataaag tgaccagcca gtaatcattt gaataataag cactggaata tataccatag
     4321 aactagcttg aatcaatgaa ttaataaaaa attgaataat agttaatcct cttgaataat
     4381 ttcctaaacg aagtcccata tattcatata tagaggttat ttttaattta tagataactg
     4441 gggtggtaat gctcagtgca aaaaatattg ctaaaggtac tgtaatatta accattactg
     4501 gatacattcc atctacatat gcccatccag gaccacctat aaagccattt gcactaatca
     4561 tagtagctgc aactgataaa gcaattggaa tccatggcat tgacctacct gccaagaaat
     4621 aatcttcttg atttttatta tcttttcctg aataataacc aataactaac attaatagca
     4681 aatatcctat tataatacat acatcaattg atgaaatcat aagccacccc tttctaagta
     4741 acaaaataaa ttcctagtat tttatttttt attatttaac gtctctggta agaataacca
     4801 tacaatagca cctgctacaa agactgcgat tgatatgtaa aagatagtca tcatacctaa
     4861 agtaccagaa gctgtaccag ctatagcagg tattgcaaac gtagatataa tccttcctcc
     4921 attaaatgac ataccagatg acaacgaacg atatttactt gggaacaact ctgtgtagta
     4981 tgctccccat gctccagaat atccaacagc aacacctagt aatactgata tcatatagaa
     5041 tgttgttgta ttatctccac ctaattttgt atatataaaa acaaatactg ctgctaatgc
     5101 catacctata aaaggtattt ttcttccaaa tttatctgca attgaacccc agaataaata
     5161 tcctactaat tgaccaattc cttgtaaact atatatagac tcagccactg ctgctgacat
     5221 tcctattcct gtcactaggt atgtagttgc attatttgaa aatgctgaat atgctgtaaa
     5281 atttgcacct gataaaagaa tcgttcccaa tccaatccat aagtattttc ccttatacat
     5341 acttatatag ctaatctttt caccagtttc ttttcccttt ttaactaatt cctttgtata
     5401 ctccaaatca tctggcacaa agaagattgc taagatagct ccaactatag gtggtataat
     5461 cgcagtatac atcaatattt gccaatttgc atcacctaaa acgccatata ctttcccacc
     5521 aaaaatcatt gctaatgaaa ataaagcagt taatatagca gctaacttac cacgatattt
     5581 acttggaaac atatctgcaa ctaaaggcat agctgttcca aatacgcctc ccaacactac
     5641 tcctgtacac aaacgtgcta ttttccataa cataatattt gtaggagata acattggtat
     5701 aaatgttgtc aatccaatca gtaaagcaac gctaattaaa gtcctcttct tcccaatttt
     5761 tgtagaaatc accccaaaaa taaatgtacc tggaattgta ccaaaagaca taactgcaaa
     5821 tatttcttct gtttgtttta atgaaaaacc aaatccccca tctgctaaag cataaatcat
     5881 tgttggttta ataaaagtac ccaccataaa attgtagcaa taaaaaaagt atattaaagc
     5941 aattgaaaaa aatgcaattc ctctttttag gtttgaatag tcttttccta atatgatatc
     6001 attttctatt tcaaccgttg ttttcatatt tttataattc ccccttttaa atttagtcat
     6061 aattttgcta aaagtttaaa catctatttt tcatatacat tccccactta aattttataa
     6121 agcaatactt ccctatatat ttcaaaacta tttatactct aaacagcttt attcttctac
     6181 tcccatataa atttgtttta atacatccat accatcaaaa gttatccact cctcctttac
     6241 tttaccttgt agtatatgat aatgattgat tcccattact tctatatgct ttccagaagg
     6301 ctgtccaaaa aatcctatac cttcatttat tccacgtaat ctccatctta cagatacatc
     6361 ataaccttca tttttttctc tttgattaca agttactctt tccacttcat aacttccatt
     6421 tggaattgat gcaaataaac ttacaatcat tccttgaatc tcatcatagc catttaaatc
     6481 tttatcacat ataaaatgta ctactgcatt atcatgatag tattttttaa cttcatttat
     6541 atatttataa ttataaatac ggcttgtcat ttcaagcatc atttcgccaa cagaatcatc
     6601 ttttgcttga tacttagttg gcataaattg accatccata gattcacaga taccataagt
     6661 tgattgtaaa ctaagcactt tagattcact tgcctttgcc atactctttg ctacttcctg
     6721 agggtttaat cctaattgag taacaatcca taaattatct ctaaccaacc attcttcatg
     6781 gattctattg ttagttgtgg cacaatcaat tacagttcta aagtttattt ttctacctgt
     6841 tgctggtcca aaattactat cacctaagtt agttgctgta gacaaaacac ggtgagaaga
     6901 taaaaaacca tcagcaccaa agttagacca tattacattt tgaccaatta atcttctatc
     6961 aggaaacgca tgtaatgttt gaagagtatt ggaaataaca tctttaatac caactaaatt
     7021 tgaagagcca caatgcattg taacattatt gtgatatgta tcataaataa cacctatacc
     7081 tttttcttcc cagatattat gtgtaatctt cataatataa tctacaaaat ctctatactt
     7141 agaatcaaat ccaggtattt cctgttccct ttttgtctgt tttgaaaaat cattgtaatc
     7201 cattactcca acaggcgtta cttctaatga atctccatag taaatttttg tagatgacat
     7261 attatcttcc cccttaaata taactttttt atttttttca gtaagagtat ttgtatttaa
     7321 tttatctgta tccactgtat ctatgttaac tttatttatg tcggatttat ttgtgtttgt
     7381 tttatctata ttccctttat ctatatttcc tttatctata tttcttttat atgtgttcgt
     7441 tttattctca tttgtttgat tttcatttat attactaaaa ttagtttgat tcattttagc
     7501 tttgttttct ttaaccgtac atgattcaag cccacttgta gaaacttctt tttcttcctc
     7561 tttacttgtt gcattatttt tgttggtaga tgccacttta tatccccctt tgttttagaa
     7621 tacgtatgca taactaagca ttaaaaatac ttttagttct ctcaagaata tcaagccctt
     7681 gttgtttcaa ccaataagga atatcaatta acacccagtt ttcttgaagt ttatcgccct
     7741 tacgataata aacatcaaca acttgcatat ctgctttaac ctctccacct ggtaatccta
     7801 aaaaacctcc acatggtaca ttagtcagat taggccatcc aaagaaacaa gcaaaattac
     7861 cttctgcaaa tctacataca tgcccattaa aagtctttcc ttttaaatta tttctaaatg
     7921 gtagctggtg ttgctcttga tatctaggta tagtataaga agcaccaatt ccacctggac
     7981 cataccaaat catatcttta gcccaagatt tttcaagtat ttctggtggg catcccatag
     8041 caccactttc atttagttca gataaatcat ctaccatttt atttactaat gctaaagttt
     8101 ttactccttc aacttcatct gcatcttcaa aaagtaatcc attatggtca attggccctg
     8161 gatatacaaa atatgaacct gtttgtggtg gcaatggatt aactcctgct tgaatcataa
     8221 acccaattat atctacaaat aatccagttt tagcaatttt accatcttga acacagttaa
     8281 attctgcata tcttaaacta atcattttac gagtatgttt aatgccaagc caatcgttat
     8341 caaaaagtcc cataaaatgc cccatactca ttacccattc actattgtca atctcatttg
     8401 ttcctgcaat gaaaatatct tgtcgtcttt gcatattact caatgatttt tttaaaggct
     8461 tccaaaatac atctgctaca ttttcagtgc cttcttgttc acgaaatgga tacacacctt
     8521 tccagttata atctgatttc atatgttgct ttaagacatt ttcaacctca tccccagtag
     8581 cattttctaa tgcttcaaaa taatttctta caactttttt tgaatcctga tattttccca
     8641 taattatctc ctcccagatt tatttaagtt ataaattaac tttctcattt catgcatacg
     8701 atttcctttt taatgatttt ttagctgttt taagtttatt ttatgaatac atatgcattt
     8761 ttctaaatat taacatatat ctaaaaaatt gtaaagaaaa tgttatcata aaaatttaat
     8821 ttttgactta tatctattta aaacaacaga aaaggtttaa aacgtaatat tctactgggt
     8881 ctaccatact tgctcataag ctgttcactc actacctcac aataaccaga atcacacaat
     8941 ctaagtataa tcctgtccat agttctaata cttacaccac atatagtagc taattccttt
     9001 gatgtaaatt cagtttttcc actatttatt attttattga atactttata tacagtatta
     9061 gcacttagcc ctgtctctgt agcaatttga taaaaacgtt catcaaaact atttttctct
     9121 ttgacagtag gatttggctt tagtggtccc ataatttctc cattttcaaa aactacatat
     9181 gctgcatcat ttttttgact ttgagccatc ttaatactct cataagcgtt aaatttagct
     9241 tcatttgctg tttttccata accaattcct atataagtgt ttataactga aacttcacga
     9301 atcatatcca acaaataaat atctctgtat ttctttgttt ctaactcaag tattttttta
     9361 gttgtaaaaa tcataaattc acttctactc tgctctacca aaacaccatc tatacggcta
     9421 ttgaaatggt atagtttatc aagaatctta atcctttgtg aaagataagc gtactcatct
     9481 tccttcatta gtgaatattc tgaaggcatg ttcagtttaa tagcaagaac aataatctga
     9541 ttctcattgt ttttctttgc aatataacgc atctgaatgt tttttatact ttgcaccatt
     9601 acgtcctcag aaggcaaggc taaaactact ggaatattat cttttttcat tatatgatag
     9661 gcctcttcaa gacctgtaat acaacaacat acatcattat tttgataatt atatttatga
     9721 aattcaagca caaactttat atagtcctct tctaaatact tttgctctgc aaaaaacata
     9781 tgttcatcct taaattcaac tccaatttct ttatacaatc tcttcatcat agccttatca
     9841 taagtatcta tactaagtct actaatatcg aatttaagca aatatgtaat ttcaagcaac
     9901 actctgtaca atgttgtttc atgtctagga atataatcag acaatacaaa atctttttgt
     9961 cttttttcaa aaaatttaaa tggcgactta cctgtaaaaa gaactgcatc cactctacct
    10021 ctatattttt ccaataaatc aggtacttca atatatctat catatctaag aaaatctgct
    10081 tcaatattag gaaaattctt tttacttact gctagtattt tatcgattac catacctggt
    10141 cctacataaa gtattttcat ttcgtctctc ctttcctaat gatactatta tctcttaaaa
    10201 cattttctaa acctaccatg actaaagcca caggactcct gattcataga attttgaata
    10261 atcaattata cctaatataa aaagttactt taagtttttt aattacgact atctgttgaa
    10321 acatttttgt atttaaattt ttgtatttaa atcttattca ttaatattaa ttacatttta
    10381 caattaatat attgtttttt ctacttaaag ttaaattctt tagaaaacac aagtagagta
    10441 atctttatta atttaacaaa aatagccttc cttaatattc aaagaaagac tatttttata
    10501 aaagttctta atgtattcta ttttgctatg ctaagaggat ttttaattac acgtccagca
    10561 catacatcag tttgctttcc tcctgccaat gcaattttac cattcaccaa cacatattcc
    10621 actcccactg tatactgttt aggattacta taagtcgctt tatcttctat tgtatctggg
    10681 tcaaatacca caatgtcagc aaaataattt tctttaagta gacccctttc tttataaatg
    10741 cctaagcgtt ttgctgaaag ataagttatc tttttaactg cttcctcaaa agtcaacata
    10801 cctttttctc taacatagta agagaaaatt cgtccaaaag taccaaatgc acgaggatga
    10861 ggctttccaa gagaaagtaa accttctgta gaaaggtcca tactatcaga acctatacat
    10921 gactttggat ggcaaacaac cttatctgta tcctcttgac cttgtgtatg aacaactact
    10981 tgaattttac ttttttcttc aatcaaaagc ctaaagaacg tatcaaatgg attttcacca
    11041 ttgattttgg ctacctccat taaagtttta ccctctacat atttatttgc ttcagtcatt
    11101 acagaggcta gtacaacacc gttccaccca cctgctaatt ggtagaaaca ttgccatcca
    11161 ggaatccctt cctccaactc ttttataatt ctgtctctat tttcttgtac ttctaatcgt
    11221 tttaccatat tttctacacc gccttcaaaa gcccaaggag gtaaaagtgc acccaaagat
    11281 gtcattccag ctgtatatgg gtaacaatca aaagttactt ccaaaccatc tgcctcagct
    11341 tcatctagct ttttaaatac ctcatcaata gttccaaaat tcttatgacc cataactttt
    11401 aaatgagaaa tatgtagagg aactcctgca gtcttagcta tataaattgc ttcatcaaga
    11461 gcttccattt cttcatcacc ttcatctcta atatgagata tatagtatcc attgtatggt
    11521 atcaattcct tacataattc agccatttct tctttctttg tgtatgctcc tggtaaataa
    11581 actaatcctg atgaaagagc tacacatcca gcttccatag cttttctaac ttccaacttc
    11641 atatttttaa gttgctctaa agtagctggt tcctttgaaa atcccatttc attgatacgt
    11701 acaggtcctt gtgctacata agctacaaca ttgactgctg gagaattttc agttaagtaa
    11761 tcaaagtatt cttgcattgt attccaatga agctctaatt ttgttggaat tgtaccaatt
    11821 atcctagttc caagataatc aattaactct tgcttcttct cttctcttat cggagcaact
    11881 cctataccac agtttgcaat aacatctgtt gttacacctg tatggatacg gcttcttgtg
    11941 tatttatcat acacaagaga taaatcagaa tgagtatgga aatctataaa accaggtgat
    12001 acaaattttc cagttgcgtc tatagtctct ttagcctctt tttgacttaa atctccaata
    12061 gccatgattt tgtctcctac aatccctaca tcacttttat atcttgaatt tcctgtacca
    12121 tcaacgatta ttccattgac aattttaaca tcaaacatgc tttttctcct ttcaagactt
    12181 atttatattc atatttctta ataaacaatc ctattctttt ttctcaacaa ataattctag
    12241 tctttctcct taataaacaa tcctattctt tttctcaaca aataattcta gtctttctcc
    12301 ttaataagca atcctattct tttgttttat tgttatcaat tacaaagaag acccttgtga
    12361 taagtatccc acctaacaca ataaacaatg ttaataatcc acctggtata ccaatttttt
    12421 caccaaagta cataattgct aacgctacag caatcgtaca tccaccagct cgaatatcct
    12481 tacgtgcaat atcaacataa attgctccaa ataatgctgg aagtatataa ttaaatgaat
    12541 tagtaacaaa ttcaggtaac aaaggaatta ctttatagcc tacaatggta aaaatggtaa
    12601 tcatagaagc tgatacaaag attgaacaac atgtaccaat tgttccaaca acttcaccct
    12661 catgagttcc tgcttctaca cctgcaattt tctgtgccat tgatgctgca ggcatacgta
    12721 tatctccaac agaacctgca agccaaccta tataagaacc tggagctcct agtgtaggat
    12781 aataagctat aggttgaaca atccaactaa ccccataagt cgcagcgact aacccccaaa
    12841 tttgaaatat aacagaaagc attggcatct gaccaaagcg aatacctaca tagatagcgg
    12901 gaataaagtt tgcaataatt gcaagaccta tagaaattct ccctattttt atcatctttt
    12961 tatcatacat aattatcacc tcttatccgt tttaatcacg ataatataat ctgtgtacca
    13021 aacataccaa ttaacatggc aatacctaag gataattctt gtaatcgttg attcttttta
    13081 aataaagttg tcaatactag cataactata gctgaaataa ttgctggtgt aagttttggt
    13141 atttcaaatg tttttatttg atttgaaaga aggtaagcaa ataatccaac tgtagcacct
    13201 gcaagtagtt ttttaaccca agtaggatta tacttcatgt tcatcttttc aacaattcca
    13261 cgcattctat gacccaaaat aaatacaaca aatagccaac ctaagttatt taaagccatc
    13321 ccccatagtg catacgaaaa agcttctgct ccaaatgcag cactacctac ttctacccca
    13381 gataaattgg atgtaagaga tattactcca agctctgttc tagctgctcc gacatcacag
    13441 agcctcatcc aagtcgtagg agcgccaatc acagctacca gtgataataa tgtaataaca
    13501 ggagatagag aaggtccaat ggctgtaaca ctagctgagc gaattgctgc tttaattcct
    13561 ttcttttcaa taccaagatt tgaagcctct tttagtgcct ctttcataaa tacgattgct
    13621 tgaacaactg aaataattac taaaaaacta gaaacaatcc aaagacctgg gctattaatc
    13681 atttcttgaa acttcatact ttattcctcc ttaaaataaa tttttcagca ttttattgtg
    13741 agctttcttt ctgtaatctt gttatgtttt tatattgttt taatttattt tatctaagat
    13801 atagtatctt taataggaga ttatctgtct taaagtctca ctaaaaacat taatacttaa
    13861 atattttatt cataattagt aaatatcaga aaattcacat attatttttt taacctgtaa
    13921 ttaacttata aggtctttta ttattttgac atttcaatat aaaaatttta atcaaaaaac
    13981 tttattattt taaaaaaaat tattattttt ataatttata ttttttaagt ttatttatgt
    14041 tcatctctat ctaaaataat gacatttcta actcttaatt ctcatttaag tttcattgtt
    14101 ttttcttaaa agcctacttt tagtcaagat actgttatct ttactcatta tttaaagaac
    14161 ttattttaaa tctgtacatg aagtaaataa tccaaaagca ttgttattct aactactcaa
    14221 cattaaatac ttactatttt ctaattactc aatataaaga cttactataa aactaaatac
    14281 ataaatggct tctaaagttt aatatatcta catggcttta tatatgacag gaaaaatact
    14341 agaaaaaata tattatagaa tacttgtata taataggcaa aactttcaaa cttaaattta
    14401 tcaaataatc ataaaaaaag tgaaccatct atagtaaaat tttatctaat aagtgaaagg
    14461 caaaaaagta gcgctacgat ttgcatagaa gggaaggaac ttagttggat aaaactacat
    14521 ttacaaacaa tatacttgaa tcagaacaga ctttgtatcg agtatctaag tctattctag
    14581 gaaatgacca agactgtgaa gatgccgtca acaatgctat tttaaaagca tatgaaaagt
    14641 tggattctct taaggaagaa caatacttta aaacatggct gattagaata gtcataaatg
    14701 aatgtaattc attaagacgc aaacgattaa aatctctatc atttgaagat gtcttcaaaa
    14761 acaaaaaaat tgatgaaaaa gatgactata gcgatttgta tactgctatt caaagtctac
    14821 ctaaaaaaat taaaattcca atagtccttt actatattga aggttattca gtagatgaag
    14881 ttaaggaaat attagatata cctcaaggaa ctgtaaaaag tagattatca agaggaagaa
    14941 gattattaaa aactaaatta gaaaacacag aggtaatcat atgaataaaa atatattaaa
    15001 taattcgttt ccagaagttc cagaaagttt tcataatagt ttaagcaaaa cactaaatag
    15061 tttaccagaa agagaggaaa attataaaat gattaataat aaaacacaca aattaccatt
    15121 tagaaaaggc ttagttgcaa ctttagccat tactttgata ttgagcacaa cagcccttgc
    15181 agtaggacat atatatagtt tagttggaaa atcaagtact aaggctacct atacatctat
    15241 cccaactaca gaacaagtaa aaaaagactt taactttacc cctaatatag tttctgaatt
    15301 tagtaatgga tataaattta aaggagcata tactgtaaat aaaaaagcat ttgacaaaga
    15361 aaataatgtt ttaggaaaat caaaagcact tagttttgaa tataaaaatg gcaatgatga
    15421 aatatctctt gacacagcaa ataatgtact tgggaagagt tctgacaatc taaaagttgt
    15481 agatacttac aaagatatta atatttctta tgaagcattc gcacaaaagt ttataccaga
    15541 aggttataaa atgacagaac aggataaaat tgatgaaaaa tcaggcaaat acacatttag
    15601 tgtggggcca aataatgaat cagacaaagt tgaagttaat gaatttaagt ttttgacatg
    15661 gaaacaagat ggagtttact attcttttac agttcaagac tcaaatttat ctatgaatga
    15721 cttagttaaa atggctcatg aagtaataga tgcaaaataa acataagtaa taataagttg
    15781 tttatgagtt tattatttat gactttaatt tttttattag taaacatggt tgtatcttcc
    15841 atctttatat tttctttaaa tttaatatat aaaattttag atatttagct accttcaaaa
    15901 gaagcattat taaaaagccc ttaaaaatat ttcttttata tggaatttta agggctttaa
    15961 ctttatttta tacaagtgta tatggaattt taaagacttt aactttattt tatataagtg
    16021 taagctccat ttgaattact tcttctttta caggtctatc attccatgta aaaccactag
    16081 atattgtatt tacctctaca aaccctaaag atttataaac atgaatagct ctactattac
    16141 ttttaaatac atcaagctca attttttcat acccatattc ttttgataaa tcaataattt
    16201 gttttaaaag actacttccg ataccttttt ccctatactc tttaagaata cccatgccta
    16261 aataaccaac tttttcagtt ttaggtaaaa cgtcacacca accaatacag ttatctgatt
    16321 ctaaatcaat tataaacagt tgtggtaagt ttttatttat aatatttttt ataaattcaa
    16381 ctgtttcttc aaaaggaaag gcttcatcca tagctagata tttcctctct tttgctattt
    16441 tatcaaaagt ttgccaatac gaataaatat acttttcttc tgcctttata aattttatgc
    16501 cttctatcaa cttaacattt ccccttttta atttttgatt gtagttaact tattttatat
    16561 gattctagtt tataatgcta tataaattac tatatattag cttaaaatat ctttgtatat
    16621 ttctataaaa ttaaaaccaa tatatctatc tcttgcatct ctattaaaat aatttttaat
    16681 a
//
LOCUS       DAMJYX010000075        16599 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJYX010000075 DAMJYX010000000
VERSION     DAMJYX010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.076"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            182..850
                     /locus_tag="PC479_002584"
     CDS             182..850
                     /locus_tag="PC479_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805119.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            852..1337
                     /locus_tag="PC479_002585"
     CDS             852..1337
                     /locus_tag="PC479_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2805120.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1398..2519
                     /locus_tag="PC479_002586"
     CDS             1398..2519
                     /locus_tag="PC479_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2805121.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2800..3990
                     /locus_tag="PC479_002587"
     CDS             2800..3990
                     /locus_tag="PC479_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2805122.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4124..4379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4438..5439
                     /locus_tag="PC479_002588"
     CDS             4438..5439
                     /locus_tag="PC479_002588"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2805123.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5543..5932
                     /locus_tag="PC479_002589"
     CDS             5543..5932
                     /locus_tag="PC479_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2805124.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6093..7301
                     /locus_tag="PC479_002590"
     CDS             6093..7301
                     /locus_tag="PC479_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2805125.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7387..8463)
                     /locus_tag="PC479_002591"
     CDS             complement(7387..8463)
                     /locus_tag="PC479_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2805126.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8692..9219
                     /gene="hpt"
                     /locus_tag="PC479_002592"
     CDS             8692..9219
                     /gene="hpt"
                     /locus_tag="PC479_002592"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2805127.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9308..10240
                     /locus_tag="PC479_002593"
     CDS             9308..10240
                     /locus_tag="PC479_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2805128.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10302..11528
                     /locus_tag="PC479_002594"
     CDS             10302..11528
                     /locus_tag="PC479_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2805129.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11612..11833
                     /locus_tag="PC479_002595"
     CDS             11612..11833
                     /locus_tag="PC479_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2805130.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12022..12192
                     /locus_tag="PC479_002596"
     CDS             12022..12192
                     /locus_tag="PC479_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805131.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12285..12746)
                     /locus_tag="PC479_002597"
     CDS             complement(12285..12746)
                     /locus_tag="PC479_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805132.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            12930..13397
                     /locus_tag="PC479_002598"
     CDS             12930..13397
                     /locus_tag="PC479_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805133.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13440..15203
                     /locus_tag="PC479_002599"
     CDS             13440..15203
                     /locus_tag="PC479_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2805134.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15211..15819
                     /locus_tag="PC479_002600"
     CDS             15211..15819
                     /locus_tag="PC479_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2805135.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15891..16460
                     /locus_tag="PC479_002601"
     CDS             15891..16460
                     /locus_tag="PC479_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2805136.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6490 a         1684 c         2932 g         5493 t
ORIGIN      
        1 taataaaaaa atattatatt tttaaacttg aaaagtaagt aattataagc tatttaatca
       61 ctggaataaa tttacaataa aaaaataaat acaaaggctt tatcattaag gtattggcat
      121 aagttttgct tattaaacaa gtatactaat aaaattcatt taaacaggag ggagaattta
      181 tatgagtaaa ttagaatcaa caacagaaaa agttgaggta aaatggtatg ggtatgtagc
      241 attaatattg ggagcattat ttttttcagg aatatttaaa gatgcaccag gaccattaaa
      301 agtattggat tttaacaatg tattaggttc ttttggaaca ttgggaacaa ttaatgatgg
      361 tgtaggtaca ttagctgcaa actttagagg tgatggaggt actggccctc gagatggatg
      421 gctatatgca ttgacattaa taccatcagt tatgtttgca cttggagtag ttagagttat
      481 agaccactta gatggaatga aggcagctca aaagcttctt agtccactat taaagccatt
      541 attagggtta cctggatttg caggactaac tttgatagct agtttacagt ctacagatgc
      601 agctgcatct atgactaaag aacttaaaga tgatgggtat atagatgaaa aacaaaaagc
      661 tgtattttgt gcatttcaat tttcaggtgc gtctgcaatt acgaatttct ttgcttctgg
      721 agcagcacta tttccattta taggagatgt accaatattt ataccacttg ctttaatatt
      781 aattatgaag tttgttgggg cgaacttact tagattatat ttaaataaat ttgacaaggg
      841 ggaagcttaa catgagtgag gtagcaaaaa aagaagtaaa agttaaaaaa ggaaatgtga
      901 tagatgcgtt tattggagga gctagaaatg gattccaaat aagtacaaat tcaatggctc
      961 caaatgtcat cttcggtttt gctataattt ctgtgttcaa cttaacaggt cttttggatg
     1021 taatagggac aatatttaca cctgtaatga gtatctttgg acttccaggt gttgcagcaa
     1081 cagctataat gacaatattt atatcaatgg gaggagcatc tggagtaatc gcaggtttat
     1141 ttacagcagg tcaaataagt caagcacatg ttgcaattct attgccaatg ttattgcttt
     1201 caggagcgca gatacaattt atggggagac ttttaggaac agctgagtta aaaactaaat
     1261 attatccaca tatgtttgtt atagcaactt taaatggttg tattgcaatg cttataatga
     1321 attatttcgt agtataggta attataataa aaatttaata taaatggaaa gtttaaattt
     1381 taaggaggag ctagattatg ggaactaaca aagattatat tgagtatgta cataaagtgt
     1441 atgattatct acacactata ccagaaaaag gatttgaaga atataagaca tcagagtttg
     1501 ttttaaaaga gcttgaaaaa atggggtttg aaaatattaa taaaggagtt gcaggaactg
     1561 gtattatagt aactatagat tctaaaaaag aaggaccagt atttgctata agagcagata
     1621 tggatgcatt ggaatttatc atagatggag aaaagaaaat gatacatgct tgtggtcatg
     1681 atggtcatac ttctatgctt ttaacagctg caaagttaat aaaagagaga aatttagtaa
     1741 aaagaggtaa gttaaaaata ctttttcaac caggtgaaga aaaattatat ggagcattaa
     1801 gagtaattga ttctggtttg ttggatgatg tagaagaaat ggtagggatg catttaagac
     1861 ctggacaaga ttgtaggttg ggaaaggcga tacctgcaat gtaccattgt gctagctgta
     1921 taatggaatt tacgattaaa ggtatcactt gtcatggttc taaaccacat ttaggagtaa
     1981 atgcagtaga tgctgtctgt ttagcagtga atgcaataaa tgcaataaga gtaaatccag
     2041 ctataccaaa ttctataaaa gtaacaaata tatcagttaa gggagaaact tataataata
     2101 taccagatga agcatttatg gctttagata taagggcaga aagtaatgat ataatagaag
     2161 aattaaaaga aaaagcaata catgctgtaa aaactgttgt aagttctata aatgcagaag
     2221 ctgtactaat ttccaaagat ggagtaccag cagcagaatt tgatgaagaa cttgtaaact
     2281 tggctgaaga aactataaaa gatgtattgg gagctgatgc atctataggt atatttaaaa
     2341 attctggtgg agaagacttc cattattata cacaaaaact aaaatgtaaa actacatata
     2401 ttggattagg agctgatgct acaccaggat ttcataatcc aaatgtaaca tttgatacga
     2461 aagctttaga atatggtgta gatatatggt gtagattggt tgaaaagaga ttgggataac
     2521 atccaaacta ataattctac atttaaaatt ataattatag aagttataga aaaagattgc
     2581 ttatgttaat tagaaaataa ttaatatgag caatcttatt ttgatataat gaaactgatt
     2641 ttggaatatt gaaaaaatat gatggaaaag taattatatt ttaaatctat tggacaaaaa
     2701 agtgatatta ttataatatt aagaattgta aaataatacc aaagtgcatc tagtaataga
     2761 cttaacaatt ataaaaattt tattcttagg agggaaaaaa tgagtaagta taattttgac
     2821 agagtaatag atagagtagg aacagattgt gtaaaatggg attttagaac taattgttca
     2881 acaaaagcac aaaaagatgg tttgcctttt tggattgctg atatggattt tgaatgtgca
     2941 gaaccaataa tagaagcatt acgtaaaaaa gttgaacata aaatatttgg atatagttca
     3001 aatgatagtg ataaatactt taatgcagtt tgtgattggt ataaaaggag atttaactgg
     3061 gaaataagta gaaaagatat tatttttagt cctggtgttg tacctgctgt tgctatttta
     3121 gtaagaatat taactaattc taatgaaggt gtaatagtcc aaaaaccagt gtattaccca
     3181 tttgaagcta agataaagag caataatagg gacgttgtaa acaatcctct gatatatgag
     3241 aatggtactt ataggatgga ttatgatgat ttagaagaaa aagctaagtg tagcaacaat
     3301 aaagtactaa tactttgtag tcctcacaac cctgttggaa gagtttggag agaagatgaa
     3361 ttaaaaaagg ttgtagaaat atgtaaaaaa tatgacctat ggataatagc tgatgagata
     3421 cattctgatt taattagaaa aggatttaaa catactcctt tacagtcatt atgcccagag
     3481 tacaaggata aaatagttac atgtatagct ccaagtaaaa catttaatct ggcaggaatg
     3541 cagttgtcta atataatagt gaataatgat gaactaaaaa agaaatatca agaagaggtt
     3601 acagctgttg gagttgcaac ttcaccaaat ccctttgcta ttgtagctac aatagcagca
     3661 tataatgaaa gtgaagactg gcttaatgaa ttaaatgatt acttagataa taatatacaa
     3721 tttattgatg aatacttaaa agaaaaccta ccaaaagtaa aacttgttta tccagaagga
     3781 acatatctag catggttaga ttttactgct tatggattaa atgaagtaga acttgaagat
     3841 ttaatgttta aaaaagctaa tgtattattt gatgaaggtt atatatttgg aaaagaagga
     3901 ataggatttg aaagaataaa tgtagcttgt ccacagtctt tattaaaaga atgtatggac
     3961 agattaaaga ctatatttga aaatttataa agtataggta aaaatttata aagtgtttaa
     4021 aaataaaaaa ataataaact tgacaaattt taactaatca cttatcatac tattatgaat
     4081 aaatagaaaa cataagagag ctaaatttta ttgaattaaa ctcttaaaga ctttgaaagg
     4141 aagagtagtt atagaatgta attaaagcga gttggtgatg gtgtgagacc aatatgaagt
     4201 ctatagtgaa gaacatcctg gagttgctaa ccgaaatttt aaaagagtag gcttagtcgg
     4261 aaccaaaccg ttatcatatt ggggtgtatc gagtaatatc tgtacattgt gagctgggtt
     4321 tgaatttatt tcaaactaat cagggtggta ccgcggaaaa gcaacctttc gtccctttta
     4381 tatagggatg aagggttttt ttaatggaaa aatatataaa tatagggagt gaatgatatg
     4441 agcacaataa taccaaagga ttataaaagt agtttgaacg taattgatac acaaatagct
     4501 ataaaaaaat taaaagattt ttttgaaatg agactaagta atgaattaaa tcttataaga
     4561 gtttcttctc cgttatttgt attgccagaa acaggtgcaa atgataattt aaatggagaa
     4621 aaggctgtaa gctttgatat accatttatg aataaaaatg cagaaattgt tcaatcactt
     4681 gcaaaatgga aaagattagc ccttaaaaaa tatgggtttg aagttggtag tggtttatat
     4741 acagatatga atgcaataag aaaaaatgaa gaattagaca atatacattc tctttatgta
     4801 gaccaatggg attgggaatt ggttatagat aaaagtagta gaaatgaaaa gactctaaaa
     4861 gatgttgtaa aaagattgta tggagtattt aaagatactg aaatttttgt atgtagtatg
     4921 tatgaaggta taaaagaaat attaccagaa gaaataactt ttataacatc acaagagtta
     4981 gaagatatgt atccagaatt atcaccaaag gaaagagaag ataaaattgt aaaagagaag
     5041 aaagcagtat ttataactca aataggaaaa acattaatat caggagaaaa acatgatgga
     5101 agaagtcctg attatgatga ttgggaattg aatggggata tattatttta caatcctgtt
     5161 ttagattcag cattagaatt atcttcaatg ggaattagag ttgatgaaga atctcttgat
     5221 agacaattga aacttgctgg atgtgaagag agaaaagagt ttgactatca taagatgctt
     5281 ttaaatggcg aacttccgta tacaattggt ggaggaattg gtcagtcaag aatatgtatg
     5341 tatttcttac aaaaagctca tataggagaa gtacaagttg gagtatggcc acaggatatg
     5401 attcaaaatt gtagtagtgc aggaattgaa ttattataat ttaaatataa actaattgat
     5461 aaaatttata tctaatatgt tcatcaaatt aattatatcg gttataatgt agaataacct
     5521 ataattataa gaggtgataa atatggagtt tttaaaaata atcattaata tagttttaga
     5581 tattttaaaa aaaattttgg tcagatttaa aaatgctaaa tttggtttgg tttttgtatt
     5641 tgatttactt aaactacctg attttatgac agataaaaga atcaatatag ttgataaaat
     5701 aaaggtaatt tctgtattaa tttttactat atcctacttt gtatctgggg ttgatataat
     5761 tccagagatg attgcagggg catttggttt tatagatgat gctatagttt taatttggag
     5821 tataggtata gtgaatgaag aaattaataa gtatagagtt atcgcaaaaa aagataaaca
     5881 ttcaaatata attgaaaatg tagaattttc tataaaagat gaagaagaat aacaatttat
     5941 atatgttatt ctttttttta ttaacaaaaa atggttaaat tgttgacgga gaggtaaaac
     6001 tctattatca taataagtgg atatccaata tttaactaag tattcaatgt gcaagaatgc
     6061 ttaatttaaa aaagttatgg agggtaaata ttatgaagaa aaaaataggt ttagtgccca
     6121 aactaatcat cggtatcata gtcggtattt taatcggaag ttatgcacca gaaataatag
     6181 tacaaatatt agtaacagtt agtactttat tctcagcatt cctaaaattt gttataccat
     6241 ttataattat aggttttgta acagcaggta tagctgattt agcaacaggt gcaggaaagt
     6301 tattggggat aactacggga atagcatatg gttctacctt aatagcagga ttattatcat
     6361 ttgcagtatc tacattaatc ttcccaaatt ttattgatgc aagtgttgca agtcaaattg
     6421 gagacccaga ggcaggaatg ttagctccaa tattcacaat tccattagag cctatggtag
     6481 atgtaacagc tgcaatcgta tttgcatttg taatgggact tggaatatca gctttaagaa
     6541 atcatggaaa aggagagact ttatttaatt tcttccaaga atttcaagga atcgtaacga
     6601 aaacgttgtc aacagtgatt atacctttat tacctttata catagcagga acttttgcaa
     6661 atataactat agcaggggaa gtatggacaa tattaggggt attctggaaa gtatacttag
     6721 tagtaatacc tttacatttt gtatatatgg catgtcaatt cacagcagca ggtgtatttt
     6781 caggaaaaaa tccagtaaga atgttaaaaa atcaagtacc tggatattta acagctatag
     6841 gaactcaatc ttcagcagca actataccag ttaatgttgg ttgtgcagaa aaaaatggag
     6901 taagtaagca aataagagaa tttgttgtac ctctttgtgc aacaatacat ttatcaggta
     6961 gtatgatatc aataacagga tttacaactg cagttttaat gatgaatggt atgccacatg
     7021 gaataggtgt attattccct tatattgcaa tgttaggtat agcaatggtt gcagcaccag
     7081 gagcaccagg tggggctata atgagtgcac ttcctttcct accaatggtt ggaataccat
     7141 cagatggtgg gcttgcaagt ttaatgatag ctttatactt aactcaagac agttttggta
     7201 cagcatgtaa tgtatcagga gataatgcaa tagcagtagt agtagataaa ataaatgatg
     7261 gaatgagtaa aaaaggaaat aaagaagagc aaagtgctta gttttttaat aatcaaaaat
     7321 gagttatctc aaatagagat agctcatttt ttatggtata aaaaattata aaataaagtg
     7381 atttttttat ttaaaaattg ctttaagaga tgatattaat aatttatttt cattaggttt
     7441 taatatacat actctaaaga aatactcatc tagaccttca aatgaacaac aatctcttat
     7501 tattattttt tgtggaagca gttgctctcg aagagattta gcagtcaatt cctttgtctt
     7561 gattttacac aatatgaaat tacctttagt attgtatata tctaagcttt ttatattctt
     7621 aagctcttta agcaggtaat ctctttcagt gttcattaag gaaatagtat ttgatataaa
     7681 atctaaatca ctaaacatga tttcacccat tttagaagct attatgttta tattccataa
     7741 atctagattt ttatttattt catttttgac attagtgtta gatataagac catatccaag
     7801 ccttattcca ggcgttgaaa aaaattttga tgtacctctt ataacgaata aattagaata
     7861 atcatcaact aattgagtac aagagtatgt atctgtatca gtaaactcta cataagtctc
     7921 atcaatcatt aaaaatgagt ctgtattttt taaaatttct ctcacttcta ctttagtaaa
     7981 tgcaaatcca gttggattac taggattaca gattactacc aaatcataat ctttagcatt
     8041 gatagttttt ataagatttt ctaaatttat atgaaaatta tcttcttctt ttgcaaagta
     8101 tttttctata gaacaattaa ttttagataa ctctttttca tactctgaat aggctggaga
     8161 gagtaaaaga gcctgtttag ggttaattgt atgtataaat gatgaaatta gttctgtagc
     8221 tccactgcca agtactatat tatctataga acatttgcag taattggata tagaagtttt
     8281 taagtctatg taatcagggt caggatacat agataccata tctatattgt ttacaatgta
     8341 ttgtttggct aaacttgatg taccaaaagg atttatattt gagctaaaat ccataaattc
     8401 ttcctttgaa aagccatact tactagataa actatataaa ttagctccat ggtttgtgct
     8461 cataaaagta cctccaaaaa taaaattata taatcgataa aaataactga ttaatatatg
     8521 tacatataga ttataataca aaggaatcgt tattccaaat ttttaatgta aaaagcaaaa
     8581 aagtatggat aagctgttaa tttaggttta aaaatgatga aaaagatttt aattgatatt
     8641 ttggtatgct ataatatata agttaataga aggaaagcgg gaggatacaa aatgtacaaa
     8701 gttacaggaa aaatgttgac agaagaacaa ataaaagaaa aagtttatga attaggaaaa
     8761 aagatagaag aagactttaa aggcgaagat ttactagtgg taggtatact taaaggagct
     8821 agtgtatttg tttcagattt aataagatgt atagatttgg atgtaaatat agattttatg
     8881 agtgttacaa gttatggaaa ctcaacagag tcttctggaa cagttaaaat attaaaagat
     8941 ttagatgtag atattgaagg aaagaatgtg ttgattgtag aggatataat agattctggt
     9001 ttaacattaa gtaatctagt tgcagcttta aaaactagaa atccaaaatc tttaaaacta
     9061 tgtactctac ttgataaacc tcagagaaga aaagctaata tacctgtaga ctatgttgga
     9121 tttgttatag aagacaaatt tattgttggt tatggaatag actatgctga aaagtataga
     9181 aatttacctt atataggtat agttgaggat gtagaataga tttaaatgtg aatcaaaagc
     9241 actaatttct aagggagtta gtgcttttta gttaattata taaaatttta gtaaaggaga
     9301 catctatatg gatattcagt taaatatatt attatttttc caaaatttaa gaaatccaat
     9361 tttgaacttt atatttcttg tctttacaat aagcacagaa gcaccgttat taatattaat
     9421 tacaacaatt atatactggt gtataaataa aaaatgtgga cagaaaatac tgtttgctat
     9481 aattggaaat tttgttgtaa atttaggcat caaagagttt gtaaaagctc ccagaccaat
     9541 aggaataaaa ggtcttgaat cattgagagt ttctactgct ggtggttatt cttttccaag
     9601 tgctcatact caaactgcaa caacattttg ggttagtatt atgactatat ttaaaaagag
     9661 atatcttcat ttaataggtg cagttatggt tttaggagta ggattgtcta ggctgtattt
     9721 agctgttcat tggccaatag atgtgatagc gggttggata ttaggaatat tttttacagt
     9781 tgtatttgta aaaatatttg actatattga tgataataaa aattatattt tacttgtagt
     9841 tttacttata ccatttataa tagtggctat atttctaaat agccctgaat attttgaaaa
     9901 atttggtata atagtaggat ttgtatttgg atatatggtt gaggatagat ttgttaaatt
     9961 taatacagat aataataaca aaaaaataaa ttttagcaat aaaaataagg ggagtaaaaa
    10021 tataattttt tcaagaatat gtaggttttt ggttgggata gtaacaatag gaatgttata
    10081 tattggcatt aagttgttta tttcaatgtt aatagtcact ttaaatataa gtgactctac
    10141 tatgagtata atttttatga attttattaa gaatacagta gttgtttttt atggaatagc
    10201 aggagctcca gcattattta aacttttaaa attaaattga ttttaaggta tagagtcaat
    10261 agtttaaatt aaaaaaatta agggtagaaa agagtgatta tttgaagaag gtagtaataa
    10321 taggtggtgg accagcaggg atgatagccg catcaactgc atgtgagaaa ggctatgatg
    10381 taacattaat tgagaaaaat cataaattag gaaaaaaact tgcaataaca ggaaaaggta
    10441 gatgtaatat tactaacgct tgtgaaatag aggaattaat agaaaatgta cctacaaatg
    10501 gaaaatttct ttatagtgct ttttatactt ttacaaatga tgatgtaata agtatgttta
    10561 ataatcttgg agtaaagact aaaacagaaa gaggaaaaag ggtttttcca gagagtgata
    10621 aagcatttga tattgtaaat gctcttgaaa gacaacttaa aagtaagaaa gtaaatatat
    10681 tattaaattc gaaagtagaa aaaataatat ctaaaaataa taaaattgaa aaagtaattt
    10741 taaacgataa aaaagaaatt aagtgtgatt cagttgtagt agcaactggt ggattaagtt
    10801 atccacttac tggttctact ggtgatggat ataaatttgc aatttcacag gggcacacaa
    10861 ttatagatac aaaaccatct ttaattggta tagaagtaca agaaagtttt acaaaagatt
    10921 tagaaaaatt atcattgaga aatgttgaaa taagggtgtt taatagcaaa caaaagaaag
    10981 tttatagtga ttttggagaa cttgaattta ctaaatttgg tttagatgga cctataatca
    11041 agtctgcaag ttgtagaatg aaagatacaa ggaaagaaaa ttacactata ttattagatt
    11101 taaaacctgc attagatgag gaaaaattgg ataaaagagt tcaaaaagat tttcagaagt
    11161 atacaaataa aaaatttgaa aaagcattag atgatttact tccaaaaaaa ctaataccta
    11221 taataataaa cttaagtgaa attaatccaa atacggttgt tcaccaaatt tctagagagc
    11281 agagaaaaaa cttagtccat ttattaaaaa atttaaagtt tacagtaaag agatacagac
    11341 ctattgaaga agctataata acttctggtg gtgtaaaagt aaacgaaata aattcaagta
    11401 caatggaatc aaaattagta gaaggtttat tttttgctgg agaagtaata gatatagatg
    11461 catatacagg tggattcaat ttgcaaatag ctttttctac tggatatttg gcaggtttta
    11521 attgctaaaa attacctgaa ttaaaaatgt ttaaaatcac ataatattat cagcaacaca
    11581 aaataaatta aacagaagga aggtaatcaa catggcaagt aacaataaca acaacagaac
    11641 agtagtacca ggagcaaaag aggctttaaa ccaaatgaaa ttagaaatag ctaatgaact
    11701 tggtatgagt aattatcaac aagttgataa aggaaacctt actgcaagag aaaatggtta
    11761 tgtaggtgga tacatgacta aaaaattagt tgaaatggct gaacaacaaa tggctggaaa
    11821 aagcaacaga tagttataaa taattattta taactattgg ctattagttt agctagagct
    11881 atctcaaata tagaaagtaa tttctaaatt gagatagctt ttttttatta aattattaaa
    11941 tagatatatt tttatgtaat aaattttatg tatttggata aaataataca aatgaatatg
    12001 ttacataagg agtgatgtaa aatgagtagt gtaaattttg aggatttaaa aaacaaattt
    12061 attaatagtg acttagatga aaaaataaga atatatacaa caacagaagg tttatcagta
    12121 gaacaattta aagagctact aaaatattat ccaatacaac atttatctaa actagaaaaa
    12181 gcacttggat agcagtattt aatccaaaat taaaaaggca atttatttaa tattaaaaat
    12241 attttttatt aaataaattg cctttttata ataggtttaa attattatac ttttgatata
    12301 taattgtaga ttctctcatt tccaagttca atagacccag tattaataag agtaactatt
    12361 ccttctatat ctctagcagt taagtattta tatggaatta ataaattata accagtatta
    12421 cttagtaagt tttttaattg ggttatttga gagatattga aacattttgc agttttagta
    12481 gatttttttc ttaagttaga taaattttca ttttgaatat ttattaaagt ttctatatag
    12541 tttctataag atatcttaaa gtttgtctca ttgactattt tttcattaag tatactactg
    12601 ataaaatctt gagacaatat attatctgca aatttatctg ctgttaattt tccagatgtt
    12661 atgccttttt gatattttct aagaattttt ttagtatagc agtatatttt atttctcgtt
    12721 tctaaatcga gttgttctat ttgcacaaaa ccacttcctt ctaaattata aaaatattta
    12781 ttaaaaatct atacttaact aatcaagctt caataactta tttacaataa atactaaata
    12841 ataaaatatt tgttattttt tattattacc aagtataaaa aatattataa ttgtaaaact
    12901 tacaaatata tgtaaaatat ttaggagtga tgaaaatgtt aaatgggata ataaagcttt
    12961 tttattctga aattgtaaaa acaaagatta ataacttaga ttcaagtgga tattctttaa
    13021 gagatgaatg taaagaaatc agaaatataa ctctaaagat agatagtgta ttttttaagc
    13081 ttttaaagtt agcttttggt tcaatattgc ttatggtagc ttttaattta aacatggttt
    13141 taggtatagg agtgttttta gttggtgtag tttatatact atataaaaga aatatggaag
    13201 aacaagtgaa agagcatata aataatgtga aaaataatat cgacatctct aaaataactt
    13261 ttatggatga aaaagggaga acaggtctaa atgcattaat tacattactt cttataggat
    13321 tattgacagg atttaactgg atattagttg ctagttttgt cgttgttttt gtttttacta
    13381 taaagaatgt atgttaaaat actaataaat agtttaatat atagggggag aaattgagta
    13441 tgactaatgg cagttataaa atgaagaatt tagtggaaat atccgctatg gtgactgaat
    13501 ctacagattt ttttaatata aaagatgaca taatagaaaa aatgttagaa gttgttcacc
    13561 ctacaaaggc atgtgtgaat ctattttata aaaatgattc taaatatgcc tacttagtat
    13621 gttcgcaaac cttagaatat gtacctcaaa tttttccaat aaatagtctt aaaggtgcaa
    13681 aaattgattt tgacacatat ccagagtata tacatgaggc tgtaaatgaa aaaaagataa
    13741 tatatgtgga aaatgtattt gaagattcta gagcagaagg tgaacgagat ttagcaaaag
    13801 ctgaaggtta tattgggaga atagtttttc cttttataat aaataacgta gttgttggat
    13861 ttatgacttg ttttttaaaa gaggatgatt atataacaga acaagatata gattttatat
    13921 catctgttgc atcactctta agtttatcaa tagaaataac taataaaaat aataatgtac
    13981 aaattttaat tgataaacta agaggctcaa taagtgccat aaatgaagct actaaaaaac
    14041 tataccttaa taagagtatt gatggctttt tggaaaattt aagtaaacaa gcgtgtaata
    14101 ttactaatag taaagaagct ttaataatta taaatagtca tcataataag gatgctatag
    14161 ttagttgtta taatgttgag caaaaaaaga aaacaaattt atatcctatg attgatatgt
    14221 ttttaaagaa agagtcattg ggggggtatc taaataattt aaaaccatgc tcaaaaaagg
    14281 gaataaattt ggaaagttat atatattaca aacttcagga taaaaatgat actataggat
    14341 gtatagtttg tgcaaatagc aaaaactata ctagtgatga tttaaatatt cttagtatat
    14401 tgtccaaaca agtttctgtt ggtatgcaat tatatgaaca taatgaaaag gaagtaaaac
    14461 acaaagttct tgaaaacgag ttgaacatat taaataaaca acaacagctt attatggata
    14521 aaggtaaaat ggattgtaat gatgagaaag aactttattt ttaccataaa ccagcaaggg
    14581 tagtaggtgg agatttttat tatgctacaa aagtggatga cgaacatata gtttatattg
    14641 ttgctgatgt aatgggacat ggaatggtat caaattatgt tgttgcaatg ataaaaggtg
    14701 cttttaaagt attatgcaat caatacaata caccaagaga aataatgaca aatttaaata
    14761 ggatgttata tgatgaattt gacaagatgg gagtatttac aacttgtttg ataggtatta
    14821 tagatactaa agaaaatatg attactgtgt ctaatgcagg tcactatagt cctgtagttg
    14881 taaagaaaga tggcacaata gaaaataatt taaattgtaa aaagggcata cctattggaa
    14941 taatggaaga tgctacttat gaaaataata ctctttcaat ggaagattat gcaatggtat
    15001 gtatgtacac tgatggaatt ttagagataa aaaattcatc aaaagaagag tatggtataa
    15061 accgattaga aaactttcta aaagaaaatt ttagattaaa tcaacagtta atagtagaaa
    15121 atctaaaatt ggatttaaag aacttctcat caaaagataa ttatgatgat gatattttaa
    15181 tagttatgtt aaaggatagg tagattgatt atggattcaa acgataaaaa aggacttaaa
    15241 agtccagaga ttataataat aaatggaagt ccaagaatta ataaaaattg cagttttata
    15301 gcaaatgaag taaaagctat gctagaagag tgtggattaa gttgtaaaat atttaatatc
    15361 tacaatatgg atattgaata ttgcacagca tgtggatttt gtgaaaaaac agggtattgc
    15421 aagtttaatg atgatatgaa gccattatat aaaatgtttg atgattcaaa aggaactata
    15481 gtaataagtc ctgtacattt tgattcaata tcagctaaat taaagactgt tgttgataga
    15541 actcaagcta tatatgcaag taaatatgta ttaaataaac cttcaattga tagaagtaag
    15601 aaacggatag gaatgtatat tgcagtgggg gggagtaaat catatgattc acagtttaaa
    15661 ggtggacaga ttgtgatgga ttttttcttt aagagtataa atactaaact tttacacaat
    15721 atatatttaa acaatacaga tgatgtatct tatatagata atcaagaatt taaagatata
    15781 atatataaaa atttaaagat atatattgat tcaatataat ttgattttgt aggtaatatt
    15841 atgtataatt gtattgcaat aatttaaata attaaaagga gggaatttca atgaaattac
    15901 caatagcatt atctaataaa catgttcatt tgagtcaagc tgatatagac gtattatttg
    15961 gggcaggtca tgagttaact aaatttaaac cattatctca accagggcaa tatgcctgtg
    16021 aagaaaaagt ggatttagta ggaccaaaag gaactttaaa aggagttaga gtactaggac
    16081 cagctagagc tcatactcaa gttgaaatat ctttagcaga tggtttccca ttaggagtaa
    16141 aagcaccaat aaaagaatct ggtgatatag ctggaactcc aggtgttaaa ttagtaggac
    16201 ctgctggaga agttgaaatg aaagaaggag ttatagttgc ttctagacat atacatatga
    16261 gcaatgaaga tgctgctaaa tttggtgtaa ctgataagca aatagtaaaa gttaaaacat
    16321 ctggaccaag agcattagta tttgaaaatg tattagtaag agctagtgac agctttaatc
    16381 tagaaatgca tgttgatatg gaagagggaa atgcagcagg cgttaaaaat ggagatttag
    16441 tagaattaat agttgattaa tattataaat agactttatg ctagtagata ttttatttgc
    16501 tagcatattt ttaaatgtaa taaatttcta taggataaat ttaatcgatt agattaattt
    16561 ataaaataaa ggggtattat aaaaaatgta ataaaaact
//
LOCUS       DAMJYX010000076        16463 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJYX010000076 DAMJYX010000000
VERSION     DAMJYX010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.077"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..971
                     /locus_tag="PC479_002602"
     CDS             <1..971
                     /locus_tag="PC479_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2805137.1"
                     /translation="KFDINMGLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMI
                     PLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFT
                     FKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKK
                     DQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQS
                     TNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNY
                     TSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            1010..1495
                     /locus_tag="PC479_002603"
     CDS             1010..1495
                     /locus_tag="PC479_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805138.1"
                     /translation="MYLKRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(2150..2374)
                     /locus_tag="PC479_002604"
     CDS             complement(2150..2374)
                     /locus_tag="PC479_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2805139.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2471..3724)
                     /locus_tag="PC479_002605"
     CDS             complement(2471..3724)
                     /locus_tag="PC479_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2805140.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            3984..4973
                     /locus_tag="PC479_002606"
     CDS             3984..4973
                     /locus_tag="PC479_002606"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2805141.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5045..5785
                     /locus_tag="PC479_002607"
     CDS             5045..5785
                     /locus_tag="PC479_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2805142.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            5985..7136
                     /locus_tag="PC479_002608"
     CDS             5985..7136
                     /locus_tag="PC479_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2805143.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7161..7916
                     /locus_tag="PC479_002609"
     CDS             7161..7916
                     /locus_tag="PC479_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2805144.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8108..10495
                     /locus_tag="PC479_002610"
     CDS             8108..10495
                     /locus_tag="PC479_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2805145.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10726..10959)
                     /locus_tag="PC479_002611"
     CDS             complement(10726..10959)
                     /locus_tag="PC479_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2805146.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11248..11838
                     /locus_tag="PC479_002612"
     CDS             11248..11838
                     /locus_tag="PC479_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2805147.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            11952..12797
                     /locus_tag="PC479_002613"
     CDS             11952..12797
                     /locus_tag="PC479_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805148.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12794..13477
                     /locus_tag="PC479_002614"
     CDS             12794..13477
                     /locus_tag="PC479_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805149.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13474..14190
                     /locus_tag="PC479_002615"
     CDS             13474..14190
                     /locus_tag="PC479_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805150.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14287..14550
                     /locus_tag="PC479_002616"
     CDS             14287..14550
                     /locus_tag="PC479_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805151.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14522..14977
                     /gene="def"
                     /locus_tag="PC479_002617"
     CDS             14522..14977
                     /gene="def"
                     /locus_tag="PC479_002617"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2805152.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15029..15490
                     /locus_tag="PC479_002618"
     CDS             15029..15490
                     /locus_tag="PC479_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2805153.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15540..16442
                     /locus_tag="PC479_002619"
     CDS             15540..16442
                     /locus_tag="PC479_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805154.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
BASE COUNT         6567 a         1744 c         2669 g         5483 t
ORIGIN      
        1 ataaatttga tataaatatg ggactatctt ctttaattac tattataata gctggattta
       61 tatattggta tagttctaaa aagtttaata ttaaggtcat gaactttgat aaatatatag
      121 caattttgat gattccattg ttgcttataa tattatttat gcaatcattt gaatatagtt
      181 caaatataaa tatagataca agtcttggga ttgtaaagct gctattaaat acttctataa
      241 ctgaagaaat tcaagctttt cttttttcta taattggaac tatatgtgta tttttttctc
      301 tatttacctt taagaaacta attcaagctc tcgaagatga caaggagagg gcaattatga
      361 atcaacagat tcatgctcaa aaaaactata ttgaagaagc gaaatcacga ttatcacaaa
      421 ctatatcctt tagacatgat tttaataatc atttagctat tgtcaatgga ttgttgaaaa
      481 aagaccaaat tttaaaagca caagattatt taaataagct tgaaaaagta acaagtgacc
      541 tatcattttc ttgtaataca cagaatattg ttattgatgc tcttttcaac aataaattaa
      601 gtatagcaaa acaattaggc atagaggtag attgtgaagt aattataccc caaagcacta
      661 acatgaacga ttttgaccta tgtatagtgt ttgctaatgc tattgacaat gcaattaaag
      721 cttgtagagt tgtagataaa caaaataggt atttaaagct ttccactaag ttagagggtg
      781 gattttttat gatagaaata aaaaatagct ataatccaat aaataattat acaagtggct
      841 caggtatagg tcttttaaat ataaaagaaa ttgctaaaaa atatcaaggg gctataagta
      901 tagataagac atcagactat tttcaaataa atgtattgct agttatttca ttacattgcg
      961 ctagtatttg agaacaattt cattgaaaat agtatttgta agctatatta tgtatttgaa
     1021 aagagaaagt ttgatatata gaaatgagga ggttactaaa atgaaagttg gaaatataaa
     1081 tggtcaagca ccaattacaa atggtttaga tagttgtcaa agtgaggtac aaggcttaga
     1141 acaaaagaaa aaagcattac aggatgaaat agagaaagta agaagtgata aaagtattga
     1201 tggtgaaacg gccaaattaa aaatagaagc tttaaaaaaa gaaatggaag agattgaaaa
     1261 taaaattcaa gagttaaaag gaaataaagg aaaaaaaaca aataaagaag aaaataacgt
     1321 ttcagaagta aataataatc tattagagag aaaaaaagaa ccttctaata atgaaaatgt
     1381 aaaacaatta tatgatgttt ttgaaagaag caatgtagac gaacaatggg ataatactta
     1441 tcgtgttctc caagatgata aaaacttaaa agtaaaattt aatagaccta tatagtttat
     1501 ttataaaaat agttaatatt ttaattatca ccacttttta gaagaataca cactttattt
     1561 taaagaccca catatttatt tacagttagc acatttatat gtggttttat aactattatt
     1621 tacaagtcta tattgataac ttttacatag tatatgatgt ttaaatactt attcaaattt
     1681 atctatacat aaacaaaaaa agagatttta aaatctcttt ttagattgtt gacttatggc
     1741 tttttgattt aataaaatca agaagccgtt tgaattttat atgttggctg atacccttct
     1801 gatattgaaa caccattttt gtttccaaaa tcgtttaaaa ttaagaactt attatgaatg
     1861 actatttcat tatgaacaat aagcaaataa catgaatctt caatcaatgt tttaacagta
     1921 gcattaaatg cttgcttata tagttctatc gtttgtttga actgcccatt aaagtaaata
     1981 tgagtcaata acataactat atctacacaa attaatataa attttttata ctgtacatta
     2041 tctaactgtt gattatctgt attacaacat gattgaattt aaaaagccat caacacatag
     2101 aatgttgatg gcctgaaaat agattttaaa atctcttttt tattatatat taattatttt
     2161 ttaaatttat attttttgca tcagattttc taatagctaa gtcaaaccct ctaaatctaa
     2221 tcactatagg gtcattaaga ggagctatct ttttcacttc aacttcagtg ccatctatta
     2281 gtcctaaggc tagaagtctc ttcataagtt tttcatttcc agctatattg tctataattc
     2341 ccttttgtcc aagttttaag ttgtaaacag tcattattaa cacctcctaa tgaaaatcat
     2401 tatcaaactt tatatttata atatataaca aaaagagcaa attgtcaata tataacaaaa
     2461 aataaacata ctaagttagt atgtttataa aaaatagtat acatgggata ctaatagcat
     2521 ctagcaaaaa accacttact aatggtacaa ctaaaaaggc ttttggagaa tcaccatatt
     2581 tttgtgtcaa agaactcata tttgccagag cgttcggagt agctcctatt ccatgtccaa
     2641 tcaaaccact tattatgatg gcagaatcaa aatcttttcc aagtactcta aagcaaataa
     2701 atattgaaaa taggattata aagattactt gaaaagttac tattaagaga ataggaggta
     2761 ataatgtaga taattcaaat aaatctatat tcataagtga taaagttaaa aaaattccca
     2821 atgaaagttc ttttaaaaaa tctataagtt taaaatttaa ttctagtata tttatcttat
     2881 catttaaatt tctaaaaata acagccgaaa acatacaagt tacaacagag ggtaatagta
     2941 tattaaacgt tatatagaag catctactta taatttctcc tgtatttata caaataaata
     3001 tgagaagaac ctgttccaaa aatacagatg gtgttattgt ttcggagaag ttattatttt
     3061 ttatcctatt cttatttcta aaaaatttac ttccaaatct ctttagattt ctattatatc
     3121 taggaatcga caagtctata tttgatgatg gttttaattt atatttattt attagaaatt
     3181 tagctacagg acatcccaat ataccaccca taataagtcc tattgttgca gcagataatc
     3241 caaccccaac tgctccttct ataccaagat tttctattgt aagtccaaat gcagcagaat
     3301 aaccatgacc accttccata gatacatttc cacacataag ccctaaaaga ggttcaagtt
     3361 ttataatctt tgatattgta atagctaata tattttgaca aaatgccaat actccacaca
     3421 aaatccaata tctaaataat acactaccac ctttctttat aagatttatg cttatttcaa
     3481 gtcctatagt tgtaaaaaag aaggttatga aatttggcat taaatttcca cttatagata
     3541 tatctgctat attaaaatat ttaaaaaata aatttagaaa acaaaataat agacctccta
     3601 ttactggtgc aggtatacag aaattattta aaaagttaac tttattcttc aataaattac
     3661 caattaaaaa gaagaatata gatagtgtca aggtttgcac tatatcaaaa cttaaggtaa
     3721 tcaaaataag tcctcctttg taattcaaag ttttaattat ataacttaaa ttcttatgta
     3781 ttgaaatttt attatcaaat gtatattaat aaatgtatat taaaaagaat cagtagatat
     3841 aattagatag tcataaatta aattaaaata ttttaatgag atatgacttt tttagaatta
     3901 aaaaccgtag tatattgata taatagaaat aagacttaaa aatctgttga tagtattatt
     3961 tataagtaca gtaggaggat aaaatgtttg aaaatttacc taagatagat ttacattgcc
     4021 acttagatgg aagtgttaga gtagaaacca tgcttgatat agctattaaa gagaaaatag
     4081 atttaccttc aaataatatg gatgaaataa aaaaattagc aaaggtttct tttaattgta
     4141 cttcactaga tgaatattta gaaaaatttg atttaccctt aaaagtaatg caatctaagg
     4201 aaaatttaaa aagaattact tttgaacttt tggaagatgc aagtagagaa aatgtaaaat
     4261 atattgaaat tagatttgca ccattacttc acacccaaaa aggtatgagt gttaaaaata
     4321 taattgaggg tataattgaa ggaattagag aagctgaaag tatttatgat ataaaaggca
     4381 atttaatatt aggttgtatg agaacaatga cctcaaaaga ggcattatta gtaattgaag
     4441 aagggaaatc atttgtaaat aaaggtgttg tagcagtaga cttgtgtggt ccagagaaag
     4501 agggattttg taaggaatat aaagatgttt ttaaattagc aagagaatat ggatataaag
     4561 ttacaataca tgcaggagaa gctgcaagtg gagaaaatgt attggatgct ataaatattt
     4621 taaaggcgga taggattggt catggggtaa aaattaaaga ccataaaaag gcgtataatt
     4681 tagttaaaga taaaaaaata ttgcttgaat tatgtccaac tagtaatgta caaactaaga
     4741 cagtagattc ttatgaagca catccatttt atacttttta taaagataat ttacatgtaa
     4801 gtataaatac tgataataga actgtatcag atataaattt aagttcagaa ctaaatgtta
     4861 tatttgatac ttttaaacta ggattagaag actataaaat aatttataga aatgcagtag
     4921 aagctagttt tgcagataaa gaaactaaag agtatttgaa tagtttaatt taatagataa
     4981 aaatatttaa actttaaatt aagctattaa attgataaaa atacattgta ttaggagaaa
     5041 aaatgtgaaa gattatgaag atgttgttta tattaaacta gtcaaaaaag aacttgaaga
     5101 atggaagaaa aatatattaa agaaaccttc tatatttgac aaagcatcaa agggtataca
     5161 gaataaattt aatggagtat tgccagataa atatcatgaa ataattactt cttctataaa
     5221 agtaatgaca aaaaccgtac tgtttggata taaatatgct acaaagccat cacttagaaa
     5281 tatgtcatta gaagaaagag aaaaattagt tttagaaaaa gctaaaactt acaagactac
     5341 agcaatgatt gaaggggcag gaacaggggc aggaggaatc ctcttaggtt tatctgattt
     5401 tcctttgctt ttaggtatta aagtgaaatt tttatatgat gtagcttcaa tatatggata
     5461 tgatgttaaa gattatagag aaaggattta tatattaaat atattacagt tggctttttc
     5521 tagtcaacag catataaaca aaacttttaa attgatgaat aactgggatg aatatgtaaa
     5581 tagtctacca gaagacatca atgattttga ttggagaaca tttcaacaag agtataggga
     5641 ttatttagat ttagctaagt ttttacaact aatgcctgga ataggagcat ttgtaggatt
     5701 ttatgtgaat ggaaaattaa ttgataaatt atgtgaaact gctgtgtctg cttatagatt
     5761 gaggattcga gagcttaagt tataagagaa attataacat aaaaaattag ttatacaata
     5821 tatctatatg tatctaattt tgcattgata aaaacaatta tggataaaag gtaaagcatg
     5881 atagaatcta atgtagaggt ttgtatttta atggaaaaat ttagttttta ttgcttaaga
     5941 tacaaattga attacgaaga gaagtaattg ggaggaatat tttcatgaca aatttgccaa
     6001 aacaatttga tagcttcaat gaagctagac aaaaaggatt tataaatgca aaggaactta
     6061 aggaaagtgg gaaaaagatg gttggagttt tctgtacatt tactccagtt gaaattccaa
     6121 tggcagctgg agcaactgta gtaggtgtat gcggcgttag tgaagagcca atacctgatg
     6181 cagaaagggt gcttccaaga aatctttgtc ctttaattaa atcaagctat ggacatgcaa
     6241 taacagatac ctgtccttat ttctattttt ctgatttgtt gataggagaa accacttgtg
     6301 atggtaagaa aaaaatgtat gaagaacttg caaaagttaa gccaacatat gtaatgcatt
     6361 tgccaaatac ttcaaaagga gaatttgcct ataaactttg gaaagatgaa atgataagat
     6421 taaaagaaga ggttgagaaa tctttaggtg taactataac tgaagaagat ataagaactg
     6481 ctataaaaga taaaaatgaa gaaagagaac ttttaaaaga gttttatgca cttggtaaat
     6541 tacaaccatc tgcacttaca ggccttgagt tacataatgt tttatatcaa gcaggtttta
     6601 aatttgaccg tgcagaactt aaacattctt taagaaaagt tatagatgat atgaaagaaa
     6661 gatatgaaaa aggtgaatgt cctgttcaaa aagataagcc aagaatattg ataacaggtt
     6721 ctcctattgg tggaataagc gaaaaaatag ttaaaacttt agaagatgca ggggcatcag
     6781 tcgttgcata tgaactttgt ggagctatta gatcaaacga tttattggtt gatgaagaaa
     6841 tagaagatgt atatgatgct ttaacacaaa aatatataaa tataggttgt tcttgtatga
     6901 tgaataatga caatcgtatt gaattattag atagaataat agatgaatat aatgtagatg
     6961 ctgtaataga tgtagtttta caagcatgtc atacatttaa tatagaaagt tatagaataa
     7021 gagaatttgt tacaaaagaa aaaaataagc catttatgtc tcttgaaact gattattcaa
     7081 aatcagatac tgaacaatta agaactagat ttgaagcgtt tgtagagatg ttgtaggaat
     7141 aaataaaagt aggaggaact atgtttagta taggagttga ctcagggtca gtggcaacaa
     7201 agggtgtgct atttgatggt gaaaaaatta ttaagaaaat aattatacca actggatgga
     7261 gccctaaaag tacatctaaa caggtctatg agttgctaag tagtgaaatt gacaaaaaag
     7321 atataaaaaa ggtggtaggt acaggttatg gaaggggagt aatggatttt gctgataaga
     7381 aagtaacaga aataacttgt catactagag gtatttactt tttaaacaag aacataagga
     7441 ctattttaga tgtaggtggt caagatagta aagttataaa tttagataga gatggaaatg
     7501 tatttaactt tataatgaat gataagtgtg ctgcaggaac tggtcgattt ttagagatta
     7561 cttctaacct tttaggaagc gatattgaaa gtatagatac attagctaaa ggatatgaac
     7621 cagttaatat atctagtatg tgtacagttt ttgctgagtc tgaaattgta agtcttctag
     7681 ctcaaaacat ttctactgga gaggtagcag cagggatact taaatctatt gcaaataaat
     7741 ccacctcaat gttagcaaga ggtgaggtaa tagatgaagt tgcatttact ggagggcttg
     7801 caaaaagtaa agaactagta aaaatgatag aagaaatact gggtaaaaaa atttttattg
     7861 cagaagatac tcaaataata ggagctttag gagctgctgt aataggattt agataaaaac
     7921 atataaaagg aaaatgagaa gctgaattta ttataaataa attcagtttt ttattttatt
     7981 ttatttattt ataaaggaaa actttggtat aattgattta aatgattgta tatttgtaaa
     8041 tacttgatat ttagtcatat ataacattat aattttaaaa attagctcaa aataaggggg
     8101 agaaagcgtg aaaaaattta cttcaaaaaa agtaacaagg tttgttgcgc tgtttttaat
     8161 atctatttta gtaatgtcaa caattcctgt atcagctgac aatagcaata cattgaatca
     8221 gataaaaaaa gcagaataca gtgagacgta taaacaatat ctaaatgatg ctaaaaatgg
     8281 acgtacagaa aaatataatg ggattatacc aaatccatat cagttggaag gtactcaaat
     8341 ccaaagtaaa ggaagaacag ctccaacatc ttatgaccct agaaaattag gtcttatgac
     8401 atcaataaaa aatcaagagg atttaggaat ctgttgggat tttgctgcaa tggcaacctt
     8461 agaaagtttc ttaaagttaa ataattatgg agactatgat ttatcagaag aacacttaag
     8521 atggtgggct tcagatggtg aatatggatg gtctgtaaat gatatgaatg gtgctttaaa
     8581 ttatgaagct atgggatatt taacatcttg gtcaggtcct aaactagaaa aagatattcc
     8641 atacaatggt agagtatcta aagcacaagg agcaaaaaaa ccaactaata tgaatacagc
     8701 acctactgtt tttaatgtga cagatgctgt gtgtgtaagt aatgacataa attctacaaa
     8761 aaatgctata ttacagtatg gagcagtaac atctggttat tatgaagata ttaaatatca
     8821 aagtgatgac caaaattcat attattgtcc aacaaaaagt tttaatacaa atcatgctat
     8881 ttcaattgta ggttgggatg ataactattc taaagataat tttaatgcaa atattagacc
     8941 atcaaaaaat ggtgcttggt taataaaaaa tagctgggga gattataatt ctgagggtgg
     9001 atatttctgg atatcttatg aagacaagac acttatgtct gatatagata atttttcaat
     9061 caaaggtggt aaaaaaccaa atgatgatga gaagatgtat caacatgact atgcaagtat
     9121 tgtaccttta atttcaaaaa agataacagc tgcaaatgta tttgatttca atagaggcga
     9181 tgaaactcta aaatctgtaa tgttcttaac tgaaagtata ggagctaagt atgaagtata
     9241 ttatgctcca gtagtaaatg gtatacctca agaaaacaat atgaaaaaat taaaagaagg
     9301 tactgcccaa tattcaggat acataacagt tccaattgat tcttttgata taccagaagg
     9361 aaagggtgca attgtagtca gtatagaggg gaaaaatgga gagtcaacta taggttctga
     9421 gtcaaatgta ccaggatatg atacatttaa agcaaaagct aatttaggtg aaagttatat
     9481 catagataat actggaaaat tctttgatat aaatagagat agtaacttct atccgtgtaa
     9541 ttttactata aaagctgtta cagaaaagtc ttctggtgag agtatcccaa atgagtcttt
     9601 aattggtagt gatagatatg aaactgctat caaagttagt caaaatggtt tcaattcttc
     9661 agaaaatgta gttttagtaa atggcagttc aatagtagat gcattggcag caactccttt
     9721 tacatctgct ataaattcac caatattatt gacacaaaaa gaagctttaa attcaaaaac
     9781 taaagctgag atacagagat taggtgctaa gaaagtatac ctaataggtg gagaaaattc
     9841 tataagtaaa gaaattgaac aacaattaaa aagcttaaat atatcgatag agagaatatc
     9901 aggaagtgat agatacaaga caagtttatt attagctcaa aagttaaatg gtataaaaaa
     9961 tgtatctcaa atagcagttg taaatggagt gaaaggatta gctgatgcaa ttagtgtagg
    10021 cgctgcagca gcagaaaata acataccaat catacttgca aatgaaaaga gcgaattgca
    10081 aggagctgat gaatttctaa attcattaaa tatagagaaa tcttatatta ttggaggaac
    10141 agcatcccta tcaaacaatc tagaaagtaa gttaaaaaat ccaactagat tatctggtag
    10201 tagtagagac gaaactaatt caaagataat agataatttt tataaaaaag atactttaaa
    10261 aaatgcattt gtagttaaaa atggaatcaa gaatcaaaat gatttaatag atggattggc
    10321 agttggtcct ttaggagcaa aaactgggtc accagtaatt ttagttggtg ataaattagc
    10381 tgatagccaa aaagaagttc ttaaaaataa gactttagaa aaagtaactc aagttggtgg
    10441 aggagcaaat aaaaatgcat ttaaagagtt agtaaaatta aaaagtgcta aatagagtac
    10501 ttaagtatta acatatatta gtgtgtatta gtaacttatc ttaataaaag ctgaaaataa
    10561 ttacttcaaa ttattgataa ataagataat caatgattgt aaagttttgt tttcagcttt
    10621 aaatttatgt agtataagtt taaaaacata aaaaagggtt actttatata gaaatataga
    10681 aaatcttaca ttttatatat aaataaccca tatttaatta actttttata gtgttctaat
    10741 agatattatt gaacttaatg gtaaatataa tgtgtaagtt tcatttaaag ttggattaga
    10801 ttcatctata tatcttgagc aaagtgtaat tgataagcta tctgcttcta taatttcacc
    10861 atatagagga caactgctaa agtaagaatg tacttcaaca tatttattta agttttcttg
    10921 aacataatcg taaaaacggt tttttgtagt agtagccata aaatcaactc ctttattttt
    10981 tattaaagtt gaaactttta aaaagataaa tatgattact tctattagaa actaagttaa
    11041 aaaataagca aatagatatg aaaattagaa taatatatta tttatatctt aattataaca
    11101 ctagataata aaaaatgcta tataaaattc agaattttcg aaagttttta agcttatttt
    11161 taaatatact aataatacta ttgacattag atagaaaaag tattatattg actatataaa
    11221 ttattttagt caaactggag ggaaaagatg cctaaatcgt attctgataa agaaagagag
    11281 tatataataa aaagacttaa agaagaagca agattgtgta tggagcaata tggtattaga
    11341 aaaactactg ttgatgagct tgtaaaacgg gtaaaaatac caaaaggaac tttttatcta
    11401 ttttttcaat caaaagaatt gctctttttt gaggtattaa gagatataca tgactctata
    11461 caaaaagaaa tcttatatga gataaataga gtagatgaaa gtattacatg tgaacaattg
    11521 acagatatat ttatgaaatt ctataggatg gttgatagta cttcaatttt aaatcttatg
    11581 ataaatggag aatttgagat attggtgaga aagttaccag attctataat tgaagaacac
    11641 tttagacatg atgactttga aattggagag attatatctt gcataccaaa tgcaaaaaat
    11701 aaagacatag aaagctttag tggagcattt agagcagttt ttcttaccat gctatataag
    11761 cgcgaggttg gaagtaattg ttttgaagat gctttaagat taatgataaa cggattagtt
    11821 atacaactta tggaatagaa tttaaatttt gtttgctaat aacttttatt aaaaattatt
    11881 aacaaacaaa aaaattatat tttattgacc atatagtcaa aatagtcaaa aatttgaaag
    11941 ggtgatattg aatgattaaa gttgatgatt tatcatttag ttatacagag agagattttt
    12001 tacaaaatat taactttgga gtaggaaaag gggaaatact aggattttta ggaccatcag
    12061 gtgcaggaaa atctacatta caaaagattc taattggaat gataactaat tatggaggaa
    12121 gtgtcattgt aaatggagta gagagtaaaa gacactcaaa taaattttat gaaaatatag
    12181 gagttgattt tgaatttcct agtttatatg aaaagttgac tgcaatagaa aatttaaaat
    12241 attttgggtc tctatattct aaaaaactac tatctattga tgagttatta aaatcagtag
    12301 gactagaaaa tgaagcaaat aaaagggtat ctgagtattc aaaaggtatg aaatctcgat
    12361 taaattttat aaaagcttta ttacataatc cagatatttt atttttagat gaaccaacaa
    12421 gtggtcttga cccatcaaat agcaaggtta tgaaagatat aattttatca gaaaaatcta
    12481 aaggtaaaac tattatttta acaactcata acatgttaga tgcaacagag ctttgtgaca
    12541 gagtagcgtt cattgtaaat ggtaaaatat ctgctttaga tacaccacat aatcttatta
    12601 tgtcaaaagg agctattaaa gttagatata cttactttga taatggagaa aaaacttctg
    12661 aatgttttct gaacaatact gctaatgata aaaatttgaa tatgcttatt gagaaaaata
    12721 aacttttatc aatacatagt agtgaaccaa cattaaatga tatttttatt gagataacag
    12781 ggaggaattt acaatgaggc taaaaaatct tattttagga gatataaagt ttcaatttaa
    12841 gtatggtttt tattttttat atcttttttt aagtattata tatatttgta ttatcaatgt
    12901 atttccaact tttatgagag aaaaaatagc tataattatg atttattctg acccagcagc
    12961 aatgggattg ttttttatgg gagctattgt acttcttgag aaaagtcaaa gggttttaaa
    13021 ttccttagct gtatctccag taaaagtatc tgagtatatt ttatcaaagg taatttcatt
    13081 aggagtaata tctagtattg tagctatgtt tattgctatt acattaaatt tagataatat
    13141 tataatatca actataggta cctttttttc ttctattata ttttctttgc ttggattgat
    13201 acttgcaagt aaagcatcta gtttgaatca gtttattgtt ttaagtattc ctattgagat
    13261 tatatgcttt attccaccaa tactcaatgt acttcttgac acaaaatcat atgcaaattt
    13321 gtaccctttt aatatatgta tttctcttat tagtggagat aaaaatttta taatgattaa
    13381 tattttaatt ttaatatcaa ttataataat aatttatttt ataacttatt attttatttg
    13441 tagcagttgg aaaaaggtag gtggagtaaa actatgataa agattataaa tgcgtttaaa
    13501 caagagtttg aacaaattaa gagagatgct atgcttttta tagtatgtgt ttctccaata
    13561 ttatgtggag tatttataaa atttggaata ccattaatac agaatatatc acttaacaag
    13621 ttttattatc aattaaattt agagccgtat ttcttgatgt ttgatttact tttagctttt
    13681 attacacctt ttatgttctt ttttgcttct acaatggtca tattgggaga aattgatgac
    13741 agcatttcaa gatatttaat agtaactccg cttggtaaaa ctggttatct tatttcacga
    13801 tttggaatac caggtatctt agcatttatt attactatga ttctacttat ctttttttct
    13861 ctaactaaaa tttctttctt gttaaactta tcaatttctt tattagtttt attacaagga
    13921 attattatat caatattagt gatttcttta tcatcaaata aactagaagg aatggtaatt
    13981 acgaaattct caggtgtttt tatgatgggt attttagcac cattttttat tttaaataaa
    14041 gcacaatata ttctgttctt cttaccaact ttttggatat cgaaggcatt taaggaagat
    14101 aattatgtct atatgtttat ttcaattgtt atatcattaa tatggatttt attattattt
    14161 aaaaagttta gtaaaaaaat agttaaataa gaaaatttaa tatataaatg taaggttgac
    14221 ttgacagtaa tgttgaaaaa tgttacattt aagatgtaaa gttaacctta catgaatgga
    14281 gcaaatatga aaaataaaat aaagatatta agagaaaaat taggattgac acaagaacaa
    14341 ctagggagat tagttggaac atctagacag gctataaatg ctatagaaac tggtaaaaat
    14401 gaaccttcaa tatggttggc atatgatatt tcaagaattt ttaatgaacc aattgaaagt
    14461 gtatttttat ttgaagaaag tgaaagaaaa tcaagagcac agataagtag gggagaatat
    14521 tatggctata agaaagataa gaacatttga tgatgaaatt ttaagaaaaa agagtaagta
    14581 tgtagaaaac gtagataata agataagaga aatattaaat gacatggcag agaccatgta
    14641 caatactcca aatggaggag gattagctgc atgccaagta ggagtattaa agcgattagt
    14701 tgttatagat ttaggagaag gacttattaa gcttgttaat cctgaaatta ttaaacaaga
    14761 aggggaacaa attgttgttg aaggatgttt aagttttcct gaagtatggg gtaaattaaa
    14821 aagaccaaaa aaagttacag tgcaagctct aaacgaatac ggtgagaaaa tagaaattaa
    14881 aggttcaggt tttatggcaa aatgtctttg tcatgagata gaccatttaa atggtatagt
    14941 ttttacagat aaaattattg aacatgtaaa attgtaagat agattggtta ttgaatttat
    15001 aaaatatttt atagtaagga gttaaattat gagattatgg cataaggatt taattgatgt
    15061 attacctaaa aatcaattag taagtcaatg gagagagcta cttgctataa aaggttctat
    15121 agataaaaaa ggtactccaa accatctatt agtgaataaa gtattgaatt atagtataga
    15181 tgaatttaaa ttttatacaa aaattgtaca tgatgaaatg ttaaaaagaa attataaacc
    15241 taatgaactt aaatatacaa gtatattaaa atggaaaaat agaaattttg caaatgatat
    15301 atctaatgaa catagtttaa atttagaaaa tttatatgat gattggcata ataaaatgta
    15361 tttaaagcaa tgcttatata atttagaaga aaaagcaaca tgtggaggaa taccaataaa
    15421 tgaatggaat atattacttt gtaaatatag taaagattat gaattgtggt ctggaaatat
    15481 catgttttaa ttactataga ttaacattat agtattattg tatataaaga ggtgattatt
    15541 tgtataataa aataaaatat attccaataa attgtgaaat agcttgtaat aaattaaaaa
    15601 ggagtatgcc atataaatgg gatttaaata tttatcgtgg atgtgagcat ggatgtaaat
    15661 attgttatgc tatttactct cataagtaca tcaactccaa taattatttt gaagagattt
    15721 atgtaaaaac aaatatagta gaaaaacttg agagacaact taaaagcagt aaatggaaga
    15781 aagaagtaat caatatagga ggagttacag atagttatca accaatagaa gctgactata
    15841 aaattatgcc agaaatatta aacctgctaa taaaatacaa gacaccagct attatttcta
    15901 caaagtcaga tttaatttta agagattacg atttaattga taagctatct aggattacat
    15961 atatcaatgt agcatctaca attaccacag taaatgaaga tacacaaagg cttattgagc
    16021 caaatggtgt ggattcaatg agacgattta aaatgttaaa agaatttaga aaaactaacg
    16081 catcagttgg tctacatatt atgccaatta taccatatat tacagatgat tttgataata
    16141 taaattcgct atttagacat gcaaaagaat gtaatgtaca ttatgtttta cctgggactt
    16201 tatacttaag gggtataact agaggtgttt tttttgactt tgttaaaaaa gagtttcctg
    16261 aattatttga taaattatca atactatata gtgaaggttc acttaatagg gagtataaaa
    16321 atcaattgta taaaatggtt aatgaactta gatataaata ttctctatca agcagttact
    16381 ctaaaataat gaaggaaaag ttaaaaaact caggagatgt acagatatct ttttttgatt
    16441 agttttatat aaaaaaattt ttt
//
LOCUS       DAMJYX010000077        15978 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJYX010000077 DAMJYX010000000
VERSION     DAMJYX010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.078"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC479_002620"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC479_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2805155.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC479_002621"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC479_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2805156.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC479_002622"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC479_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2805157.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC479_002623"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC479_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2805158.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC479_002624"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC479_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2805159.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC479_002625"
     CDS             2662..3168
                     /locus_tag="PC479_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2805160.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC479_002626"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC479_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2805161.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3982..4770
                     /locus_tag="PC479_002627"
     CDS             3982..4770
                     /locus_tag="PC479_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2805162.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5241..8957
                     /gene="rpoB"
                     /locus_tag="PC479_002628"
     CDS             5241..8957
                     /gene="rpoB"
                     /locus_tag="PC479_002628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2805163.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            8999..12484
                     /gene="rpoC"
                     /locus_tag="PC479_002629"
     CDS             8999..12484
                     /gene="rpoC"
                     /locus_tag="PC479_002629"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2805164.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12773..13195
                     /gene="rpsL"
                     /locus_tag="PC479_002630"
     CDS             12773..13195
                     /gene="rpsL"
                     /locus_tag="PC479_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2805165.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13319..13789
                     /gene="rpsG"
                     /locus_tag="PC479_002631"
     CDS             13319..13789
                     /gene="rpsG"
                     /locus_tag="PC479_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2805166.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13838..15904
                     /gene="fusA"
                     /locus_tag="PC479_002632"
     CDS             13838..15904
                     /gene="fusA"
                     /locus_tag="PC479_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2805167.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6257 a         1989 c         3249 g         4483 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctgtata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga gtgtttataa ataaaatgta ataaataatt aattaacaaa
     3901 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     3961 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4021 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4081 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4141 ggtggttgtg ctaagtttgt ttactttaat gctcgtgaag aagagacctt tacctcaatg
     4201 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4261 acaaaccctt ctcttgacaa agaccttgta actggggata cagataattt ctttgacaca
     4321 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4381 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4441 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     4501 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     4561 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     4621 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     4681 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     4741 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     4801 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     4861 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     4921 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     4981 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5041 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5101 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5161 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5221 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5281 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5341 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5401 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     5461 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     5521 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     5581 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     5641 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     5701 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     5761 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     5821 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     5881 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     5941 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6001 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6061 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6121 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6181 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6241 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6301 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6361 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6421 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     6481 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     6541 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     6601 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     6661 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     6721 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     6781 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     6841 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     6901 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     6961 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7021 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7081 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7141 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7201 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7261 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7321 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7381 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7441 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     7501 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     7561 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     7621 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     7681 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     7741 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     7801 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     7861 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     7921 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     7981 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8041 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8101 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8161 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8221 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8281 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8341 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8401 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     8461 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     8521 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     8581 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     8641 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     8701 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     8761 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     8821 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     8881 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     8941 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9001 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9061 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9121 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9181 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9241 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9301 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9361 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9421 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
     9481 gtatggatat acttttactg taggaatggg tgctgaagct gtaaaaacat tactacaaaa
     9541 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
     9601 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
     9661 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
     9721 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
     9781 tataaataga aacaacagac ttaaaagatt attagagctt ggagctccag atataattgt
     9841 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
     9901 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
     9961 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10021 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10081 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10141 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10201 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10261 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10321 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10381 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10441 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    10501 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    10561 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    10621 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    10681 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    10741 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    10801 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    10861 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    10921 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    10981 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11041 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11101 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11161 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11221 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11281 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11341 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11401 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    11461 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    11521 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    11581 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    11641 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    11701 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    11761 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    11821 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    11881 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    11941 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12001 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12061 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12121 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12181 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12241 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12301 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12361 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12421 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    12481 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    12541 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    12601 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    12661 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    12721 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    12781 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    12841 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    12901 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    12961 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13021 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13081 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13141 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13201 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13261 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13321 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13381 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13441 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    13501 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    13561 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    13621 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    13681 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    13741 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    13801 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    13861 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    13921 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    13981 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14041 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14101 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14161 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14221 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14281 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14341 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14401 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    14461 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    14521 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    14581 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    14641 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    14701 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    14761 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    14821 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    14881 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    14941 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15001 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15061 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15121 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15181 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15241 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15301 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15361 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15421 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    15481 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    15541 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    15601 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    15661 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    15721 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    15781 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    15841 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    15901 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    15961 tctcatctag cacaagat
//
LOCUS       DAMJYX010000078        15776 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJYX010000078 DAMJYX010000000
VERSION     DAMJYX010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15776
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.079"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            94..1284
                     /locus_tag="PC479_002633"
     CDS             94..1284
                     /locus_tag="PC479_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2805168.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1454..2416
                     /locus_tag="PC479_002634"
     CDS             1454..2416
                     /locus_tag="PC479_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2805169.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2538..3200)
                     /gene="zmp1"
                     /locus_tag="PC479_002635"
     CDS             complement(2538..3200)
                     /gene="zmp1"
                     /locus_tag="PC479_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2805170.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3300..3385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3571..6489)
                     /locus_tag="PC479_002636"
     CDS             complement(3571..6489)
                     /locus_tag="PC479_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2805171.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6607..6692)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6819..7997)
                     /locus_tag="PC479_002637"
     CDS             complement(6819..7997)
                     /locus_tag="PC479_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2805172.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            8372..8557
                     /locus_tag="PC479_002638"
     CDS             8372..8557
                     /locus_tag="PC479_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2805173.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(8622..11396)
                     /locus_tag="PC479_002639"
     CDS             complement(8622..11396)
                     /locus_tag="PC479_002639"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2805174.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11568..12824)
                     /locus_tag="PC479_002640"
     CDS             complement(11568..12824)
                     /locus_tag="PC479_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2805175.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12896..14398)
                     /locus_tag="PC479_002641"
     CDS             complement(12896..14398)
                     /locus_tag="PC479_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2805176.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(14489..15679)
                     /locus_tag="PC479_002642"
     CDS             complement(14489..15679)
                     /locus_tag="PC479_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805177.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT         4949 a         2735 c         1924 g         6168 t
ORIGIN      
        1 caaacaagta atttttcgaa aattgtctaa attcagtaaa aatatggtaa tatatacata
       61 agcaatataa atctataaaa aggagtgtta catatgatat ctaataaaat gcaaacttta
      121 gttgcaaata gttctgttat aagagctatg tttgaagaag gaaagaaact ttctgatatt
      181 tatggtgagg aaaatgtttt tgattttagt ataggaaatc caagtgttga acctccagag
      241 actattaaag cagttataaa tgatatttta aatgaagaat ctccaaattt agttcatgga
      301 tatatgaaca actcaggata tgaggatgta agagatgcta ttgctgaaca cataaataaa
      361 aaagatggat taaatcttac aagagaaaat ttaattatga cttgtggtgc tgctggtggt
      421 ctaaatatta ttttaaaaac tcttttaaat cctggtgatg aagttattgc ttttgcaccg
      481 tattttggtg agtataaaaa ttatacagaa aactatgatg gaaaattaat agaagttcct
      541 acaaatatag agacttttga accagattta gatgctctaa aaaatgctat tactccaaaa
      601 acaagagctt taataataaa cactccaaat aatcctactg gtgtcatata ttctgaagaa
      661 ttattaaaaa atcttggaga attattggat tcaaaacaaa aagaatttaa tacaagcata
      721 tacctaattt ctgatgagcc atatagagaa ataatttatg atggtgccaa agttccttgt
      781 gtactaaaat actatagaaa ttcatttata ggatactctt atagtaaatc attatctcta
      841 cctggtgaac gtataggata tattgttgct aatgggcaaa tggatgattt tgatgatgtt
      901 atgtcttcat taaatgttgc aaatagaata cttggatttg taaatgctcc ttctcttttt
      961 caaagggtta ttgcaagaag tttagatgca gaagttgatg taaatattta taaaaagaat
     1021 tttgaccttc tatataatag tttaatcgat atgggatatt cttgtgttaa acctaatggt
     1081 acattctatt tgttcccaaa agctccaatt gaagatgata aaaaattctg caatgatgca
     1141 aaacaattta atttattatt agtaccaggt tcttcttttg gttgtccagg tcattttaga
     1201 gtatcatatt gtgtatctta tgataaagtt aaaagttctt taccagcatt tgaaaaatta
     1261 gccaaattat ataatttaaa ataactaaat caaagtaatt caaataatta actaccttag
     1321 tatagaatac ttacattcta tattaaggta gtttataatt ttatgtacta tattttatac
     1381 ataccatatt attccaatat gcttgaatat tcttatctat agtactaata taaattacaa
     1441 ggaggtgttt gacttgaaaa aaggttatgt ttatataata ctgactacct tattttttag
     1501 ttcaatggaa attagtctaa aaactgtcac taatgacttt aatcccttac aaataacttt
     1561 atcaagattt tttgttggcg gtttagtctt actacctttt gctgtaaaaa gattaagagc
     1621 tcttagtctt tctattacaa aatcagactt aaaatacttt gcatttttag gttttatgtg
     1681 tgtagtagta agtatgtcat tgtatcaact tgctgttcta aacacaaagg catctgtagt
     1741 tgctgttttg ttcagttgta atccagtctt tgtaatgctt ttagcttact taattttgaa
     1801 ggaaaaaata tataggcaca atgtaatttc acttatatta gaagttcttg gaattatagt
     1861 tataataaat ccattgcata caaaactaac tcttagtgga atattcttaa cattatcttc
     1921 tgctattatt ttttcaatgt atactgtatt tggtaaaaga aaaaccttaa aatttggtgg
     1981 aatagttgta acctgcttta gctttatatt tggtagtttg gaaatgttga tattggtttt
     2041 actcactaaa attaatttta tcgcagatat attaaataaa aataagcttc atgtatttgc
     2101 aaatatccct atttttagtg gatatacctt acacaatatg cccattatga tttatgtatt
     2161 cgtatttgtt acaggtgttg gttatgcgct ttactttatg gcaatggaag ctacatctac
     2221 tagtcttaca tctttagtat tcttctttaa accagtaata tctccaatac tagcattatt
     2281 aatacttaaa gaaattatcc ctataaacat ggttatagga attttattga tagtaattgg
     2341 ttcaataatt tctattatac ctacgataat tactcaaatg cataataaac acgttgaagc
     2401 cttgtataag gagtagtaat tgataatcta tcatagttat acataagaca aaatttatta
     2461 gatttaaacg caatataaaa gagatgctat tataatagca tctctttatt tatatttatt
     2521 ttaaatttat taaaactcta tttagctaaa ttttgcaaaa agctataagt ttgtggacaa
     2581 gctgatttta acttactatt tgtatcttga tttaaatagt aatatgcaaa tgattctgca
     2641 aaaaattctt ctggatatac acctaagtag tttacattac ctaaagaacg accttcttta
     2701 gcaaatattt gtttgaattg tgctgattta gatatatcat ttaatactat atggtctatt
     2761 gcatgtgctg tttcatgtaa ttctaaattt attgcatcat gccctttacc tttgttacta
     2821 aatcctattc taagagctac tgtacttcca cctaaacctg gaacatcatc ccaagttttt
     2881 ccagtacctt cccatccctt aggaactaca ccttttaaat aagcatattc tttttcatct
     2941 gttaactttc cacttaataa ttttattttt aagttatttt gctttaatgc atttaaatac
     3001 tttccatcta tattagctaa tctatttacc atagcattag cttcgttttt atcgtaattt
     3061 ccagttggaa aaactactat ttgactaagt gtgtctttat tttgttgtat agtagtacta
     3121 tctgcatgtg cacttactgg aactgaactt attaagaata ttgatattat agctattaat
     3181 aattttttac ttggtctcat attattccct cctttgagtt aggccgggag taataaattt
     3241 caaaataaaa aagataccca agagtatctt tgtatataca aaaagaaatt tttactcttt
     3301 tcggcaactg gctggcatgg tttactaaac tttagcccct atagctttgc gtgactaggt
     3361 ttccctagtt ttgcctcatt taattttata tctatattat agcttatatc tttttaattg
     3421 tctatatccc tttggaactt ttttctaaaa agttattatt tttttacata aatcttcaaa
     3481 aaaaaatatc ttctaacaat attttcatta ttaaatattg ttttagaaga tattttttaa
     3541 tataacttat attttttata atgttagggt ctaatttgta tttttatttc ttcttaatac
     3601 gataagtcct acaatagctc ctataaccaa aagtatttct ccaataattg tagtactatc
     3661 ccctgttttt ggtggtactg gtggatttac caaagtatca tctttaactt tattattttt
     3721 ttcttctgac actgatggct ttactacttc tggtattgga ataatctgtt taacaggagt
     3781 ttctatcatc tcttctgatg gtggtactgg cggattcaat acctctggta ttggcggatt
     3841 tattatatct tctgatggtg gtattggtgg atttataact atagtatcct ctgtatcaga
     3901 tgaacttgat ggtttccttg gtttatctgt gcttggtggt actggtggat ttactattgg
     3961 ttcatctgta tttggtggtg ctggtggatt tactggtgga ttttcttgtc ttatgttttc
     4021 tatatttcca atatccacta ttctaccaat atctttatta ccaatttcaa ccttaatttt
     4081 atccttcagt ttttcatatc cacttggagc cattatctcc actaaataat atgttcctgg
     4141 tttttttata ctagattgaa ttataccatc ttcatcacta actaaatcct taactactat
     4201 atttccaaat gcatcttcta aattgaactt agcacctgaa agtacatttt tatctaaagc
     4261 atccactttt ttagcttcaa aagcataacc ttgcttagta gtctctactc ctggaggact
     4321 tgaaccatta atctgagcca catttcgtat agtctcccca acttttacat ttgtgaagtc
     4381 tgcatcaaat gtaacccaac tttgcacagg ttccccttct tttatctgtt taaattcgta
     4441 tttatttact aatcttattg tattttttcc atctccagct tcttttttaa gttcaagatt
     4501 atcagaatat tttatattag atatttctag agctgagttt acttcatcta aaagatttac
     4561 ttgtcctgca tcataaaccc cataagttga catattgatt ttgtatttaa caatctggtt
     4621 atcactacta tttaaaactt ttttatcaac ttcttttttt gcgcttatta atccagaatt
     4681 tttcttaaag tttatctcat catttttatc aatttcacta ttatttgcat caagaacagc
     4741 tctatttata tagctatctt caatactaat tacctttaat gtataagcta taaaaaactt
     4801 tgattccact tcttctttac tgagattaat tgtaaatcca gttataactt tatctacttg
     4861 cccactttca tttctttttt cttctacttt atatgaaata ttattggcaa ttttttcttt
     4921 actccaagga gtccagctcc cattcattgg aatttctaca actctaaaat catgcccagc
     4981 ttctcctgaa gtagttaatc tcatgccttc tggtatatta tctgttataa caacattact
     5041 tattggtgat ttatcatcat ttattgaaat ctgatagttt attacttgac caacttgagt
     5101 aatatttgtt gctcctgaat gtcctttagt aagaagataa tcatctcctc tatcaaatat
     5161 acttacagta gcatcatctt cttgtatctt cttaccatca gattcaatcc tagctacatt
     5221 cttatattta ttaccatgtt ttgcattttt gtcactgatt ctagccttat acttaacagt
     5281 atacgattta tctatatttc caagattaac tgaaatagaa tttttactct gttcaatatt
     5341 aaaatcacta acttcttttc catcacttgt tactacactt acagaaccat ttatcaatgt
     5401 caatccttca ggaagctcat catacaaaat aatatctttt aactcttcct gcttttcatt
     5461 tacttgtata ctgtaattta tatctttatt tttaactggc ataaatacag tagttgattt
     5521 ttttccttca acaagctttt taagtgttcc tttagcatct ggaacatccg ttttatctcc
     5581 aggttttatt tctacatcta tatctccact tccactttct atttttccag tagtttcaat
     5641 atgtattttt gcattagtat cttcatcaac ctttttaaca tatgcttcca aacctatttt
     5701 accttgcatt acactttggc cattaggtaa ttctctaaat gtaagtgtat actctccatt
     5761 actttctgtt ttatcaaagc attcacttgt ataatctaaa ttaactttat tgaatacatc
     5821 tggcacctta aaagttattg aatctcctgc ttgtattcct tttgatgttg gagtataagt
     5881 taaattaaat gatatttttt tcattgattc cgtttctatt gtattgttat taatttttac
     5941 aacaaatcca tagtcttcat tttcttcatt aagttcttct tctaaaactt tcaataaatt
     6001 ttcattttct aatagattat cttcctctgg agttttttct gtatttttat tgtctagtgg
     6061 tttttcatct tctaaagctt gttctgactt tttattatct gttgacttat cttcttctgg
     6121 tatctgctct ggctttttat tatctattgg cttttctcct tctggagttt gctctggctt
     6181 tttattatct gttgacttat cttcttctgg ggtctgttct ggttttttat tatctgttgg
     6241 tttttcttcc tctggggtct gttctggttt tttattatct gttgccttat ctttctctgg
     6301 agtctgttct ggctttttat tgtcgctttc cttctcctct ccagttgatg aattagttga
     6361 accactttga atctgactat tctctcctaa ttccgaagca taagatacag ttggatatat
     6421 tgtagacacc accatactta aaatcataat taaactaatt aataatgcct ttctatttcc
     6481 tttcttcatg taaatctcac ccctttaggt tacctataat ttataatcta atcttccatt
     6541 aatatcgtat ataaagtaat ttttactaaa aaattataca aaaaaagcta tgaaaataaa
     6601 taaatttata ttcatagccg gttgcaccac tgactcagta tatccataag gtgcccaata
     6661 atagaatata cttcaacatc tctataatat ttcataaaaa caattaaaat tgctttattt
     6721 atattaaaaa tacaattata tacttctatt atattatgcc tctatactat tgtctatcct
     6781 ttttacattt tttaccttat gatgagatta attaataatt aaataaaatc cttatctaca
     6841 acaattacac aattatattc aggatttatc tccttaacta attttgttat atcttctttt
     6901 aagtcatctt ctgacatgtt tttggaaaat tcggaaggat taactactat atcaaatatt
     6961 aaattcttct tgcttccttt tccaactatt ctaaaatcat gcatagattt tacaagacta
     7021 tttttcttaa gtatttcttc aagttctttt ttggctgaca taacttcttt atcttcaaga
     7081 catattgggt ccatgtgaat tactagatat atatctaatt tttcagatat ttctctctct
     7141 gcagtatcta taatttcatg tatttccatt atatctatgt tggatggaat ttctgcatgt
     7201 atagatgcaa tacatctacc aactccatag ttatgaataa ttaaatcatg tgtacctatt
     7261 atatatttat aagataacaa tagctctata atagaattta ccatttcttc atccggtgct
     7321 tcccctaaaa gaggatttat agtatctttt acaagagaga aaccagcata aagtatcact
     7381 aatgatacta cgataccaac atatccatct atgggaaaat tagtaaatct agctacaata
     7441 aatgatataa ctacacaact tgaagtaaat acatctccta aagcatcaag tgaaactgct
     7501 tttaaggctg aagaatcaat tttatttccc ataaatttat taaaacgact caaccagatt
     7561 ttaatcatta tagatgcaat taaaagtata agtggtatta cttcaaattt tacaggaatt
     7621 ggatttacta tcctttctat agatgacttt acaaattgaa gtccaaccaa catcaccata
     7681 aatgctacta ttaatgctga taaatactct attcttccat gaccaaaagg gtgttcttta
     7741 tcagctggct tacttgctaa tttaaaacct atcatagtta ttgctgaaga tgccatatca
     7801 gaaagattat taaatgcatc agccattata gctatactag atgtgagcat tcctatgaat
     7861 acctttatca caaataaaag taaattggat aatataccta ctacaccagc tacataacca
     7921 tacttatttc taacatctgt attttgaacg ttttcgctat ctctgataaa tgttttgact
     7981 aggattttgg aaaacatgaa acttacctcc ctgcccttta ttttattaac aaattttata
     8041 atctatatct ttatttatga taacataatt tatatataaa attcttaaaa atcactctac
     8101 ttcagtaaaa tattattcta tatatctatt tagatacgaa ttttatattt agtaaagttt
     8161 ttatggctta gtgtctgttt aatagattct atataagttt ttacaaagat tatttattat
     8221 gataatatca tttttcccaa gtattactta gaatatgatt tttttgtttt gtctgtacat
     8281 attttaaatt tatactatat ttatctatat tttataacct tatgatacac tataactata
     8341 agattttaaa tggaaaggaa tgaaataaat catgtttttt atcagtgata tttacacaaa
     8401 atcacctatt aaatttgata ctcctttgca aaaagaggca tataaaatat tacaaaaatt
     8461 agatattgac tttgaatgtg ttgacacaga tgaagcaatt acaatggaag attgtgttca
     8521 gattaataaa aaattaaata tgaaaatggt aatataaaac aacattaaac aaacaaaaac
     8581 cgcaaagtta caatatatag tactttacgg tttcataaga attaattttt actattaaag
     8641 ttcatagttc tactattttt cattgttcta agaattagct ttacaaattc atttgacaat
     8701 aggatagtgg atgcaaatag tattatcttt atccacatat caaagtccaa agaaacagaa
     8761 ttaaagaaac tctggaacac ttgtgtaaat attatttgaa ttatccctgt aactactatt
     8821 atttgcaatg ccagtttatt tttaaagaaa tttggtattg tactatttaa accaaactct
     8881 ctacaattta atgcattaaa aagggcactg aatgcaaata aagaaaatat aacagtacct
     8941 tgttcttctg atgtagctcc caatatatta aaagctgatt gagtaaatac aattgtaatt
     9001 ataagaattg catttataat aatatttaca aacatagacc tagctattat tccagaatgt
     9061 ctatttattg gttttctttt caatacataa tctctaacag gttctaatcc taatgctagg
     9121 gctggaggac catccattat tatatttacc cataataatt gtattgtagt aaatggcata
     9181 tccttaccag taagttgaga tattatagca attataaatg ctactatatt tacagtaagc
     9241 tgaaactgta taaatctttg aaagttctca tatatacctc ttccccattt tattccctct
     9301 actatagtac taaagctatc atcagtcaat attatatctg ctgcattttt agatacttct
     9361 gttcctgaaa tacccattgc aattccaaca tctgcttgac taagtgctgg ggcatcattt
     9421 ataccatcac ctgttacagc tacaacttcg ccactttttt gaagtgctga aactattctc
     9481 atttttgtat ctggtttact tcttgcaaca atagatattc ctttgatttc ttctctcaat
     9541 tctttatctg tcaatacatc tatatatgtt gcttcaactg cctttttccc atcatttaat
     9601 aatcctaatt catttccaat agctgttgct gtatttatat tatctcctgt aagcattttt
     9661 acatctactc ctgcattaaa tgctttatca atggaatctt tgaccccttc tctcaatggg
     9721 tccactattc ctacaaatcc actaaaaact aaattatctt ccttcatata acttcttgaa
     9781 tttcctatta aattcaattc accttctgaa gtcattgcaa cttctgtttt agaattactc
     9841 atttttttat aagcaaatcc taatgttctc atagatttaa tttgtaactt cttaatctca
     9901 tctaaaatac ttttttctac ttttggagtt attggaacaa tattttttcc ttgttgtaca
     9961 taagaacatt tttttaataa tacttctgga gcaccttttg aaagtaatat atctgaatcc
    10021 tcttgacgaa ttaatgttga catcttcttt tcttctgaac taaatggtat ttgtgatatc
    10081 aaatacgttt gttttctcat ttcattatag ttcttatcat tatgatataa aagtaatgca
    10141 cactctgttg cgcttcctat atatttaaat gaattatctt ctttttctat atctgcagtt
    10201 gaatttacta tacagttttg ttcaaaatag ctattacttt gatactctcc tccacttata
    10261 tatttaccat caacatatgc tacctctacc atcattttgt tttgtgttaa agtccctgtt
    10321 ttgtcggaac atattacaga aacagaacct atagtttcac aggcttcttt ctttgttact
    10381 aatgcattta tctttgccat cttttgcatt gttatagcta aggtaatatt tatcatagtt
    10441 ggcaaacctt ctggaacagt tgcaactatt aatgcaatac aaaccatata tgcattttta
    10501 gcaggttcta atgattgtaa gaaagataat atactgcttg tatctaaaat taacatacct
    10561 tttctctgca tctgtataat cataaacata cacaataaag tagctattat gcctgatatt
    10621 cctgctattt tagcacccaa cttccctaat ttaatttgaa gtggagtttg ctggtcttca
    10681 tttgataaat tctgtgctat cttacccatc tctgtcttat caccagtatg agtgacaacc
    10741 attatgcccc taccatatgc tacaagagtc cctccaaata ccatattgac ttgctttgca
    10801 ggtattgcat cctgttcgat tatttctgtt ttagaataaa ctacttccat atctaataca
    10861 gcatcagcat tcttagctac atcatcagat tctcctgtta gcatatcttc tctaagtttc
    10921 aaatttatag actgtataag tcttccatct gctggtatca tatccccagt ttctatatat
    10981 acaatatctc ctggtactaa atcatttttt gatattttaa ttatcttccc atttctaagc
    11041 acctttactt ctatattttc tgttaatttt gacagggcat gagctgcttt tttagatttt
    11101 ccttcagtta ttatgcctat agaaattcca ataaaaatag cccctaaaat acctaatgcg
    11161 tcatgtaatt ctcccacaaa agaacttatt actgctgctc ctatgagaat taatatcata
    11221 ggttctgtca aactttctcc caactcgtcc caaaatgttc taccttcttt tatagtaaat
    11281 tcatttaatc catatctaag cttcctttct tctacctcat tatcatcaag tccaatttct
    11341 ggatttgttt ttaagtattt taatacttct ttggtaggtt tattataata cctcataact
    11401 ttatccccct tgttaattat ctataaggaa ttatatgctt atgatagctt gtatattact
    11461 tttcaaaaaa aataaggtgt tgattaatca acaccttata gtggttatat attattaaat
    11521 tttatgttac aggctttata aattgtgagc tatacaaatc aaaatattta atcttcaaaa
    11581 tacccttctg gatataatgg aaacttagat ataagttctt gtgcctctgc tttacattca
    11641 tctaatactt ctttattatc aatattttct gctaccttat tcattatccc tgctatctgt
    11701 ataacttctt tttctttcag acctctttga gatattgatg taagacctat acgaacacca
    11761 ctagttacaa atggtgattg tgggtcgtca ggtataacat ttttattaac tgttattcct
    11821 acatattcaa gagctttttc aaattgtttt cctgtaagat tcttacttct taagtcaacc
    11881 attactatat gattatcagt acctccactt acaatactaa aaccatgtga aatcagttca
    11941 gaagctagag tctgagcatt tactacaact tgctccatta tctttttaaa tttatctgta
    12001 cttaaatata atagtgaaaa ggcttttgct gcaactgtat taagatgtat agaacctaat
    12061 gttcctggaa aaacaccttt atcgagtttc tttgcatgtt ctgctttaca aagtacaata
    12121 ccacttcttg gtccacagaa agtctttgtc gtagatgaag aaacaaaatc tgcataaggt
    12181 acaggactag gtattacttt cgctgcgact aatcctgcaa catgtgccat atctaccata
    12241 aagtaagctc ctacttcttt tgcaaccttt gatattcttt cataatctat taatcttgga
    12301 tatgaacttg cccctgcaat tataagtttt ggtctacatt cttttgcctt tgcttcaaga
    12361 gcatcataat ctatacgtcc agtatttggg tctaccccat aaaattcata tttataaatg
    12421 tcatgtaaaa aattaactgc gctaccatgt gtcaagtgtc caccttggtc aagacgcata
    12481 cttaaaacag tgtcatttgg ttttaatatt gatgaatata cacaatagtt agctgtagaa
    12541 cctgaatacg gttgtacgtt tacatgttct gctccaaata cttcttttgc acgttttatt
    12601 gctagggttt caagtttatc tgcttcttca gaacctgctt gaaaacgagc ccctggatag
    12661 ccttcttctg ttttatttgt aaatacacat ccacttaatt caagtacttc tgttggtgct
    12721 gtactttcag aagctatcat ctcaatattg tgttcttgtc taaccaactc atcagctaca
    12781 atcttgtata actctgggtc tgaaattttt gttgttttta acataataat cctcctctta
    12841 attaaagaat atattttatt agtttaatct tcattgataa atttattata acggtctata
    12901 aaacgtaaga tattcctcca ccaggtccta atggtatgcc aatacccatg aatagtaaca
    12961 atattattat ttctattaat gtaaatacca ctgagtatgg aatcatattc gatatgacag
    13021 tacctatacc tatatcttta tcatatcttc tggcaaaggc caatataact ggaaaatacg
    13081 gaaaaagtgg acttattgga tttgttgaag catctccaat acgataagct atttgagtta
    13141 atgctggatt aaagtctagt agcataaaca tgggaacaaa tataggtgct aaaatcgccc
    13201 attttgcaga agcacttcct acaaataaat tgataaatcc agatagcaat ataaatccta
    13261 ttatcaatcc atagctaggt attcccgact ctgctagtaa ttttgctcct gttattgata
    13321 aaattgttcc taagttacta agattaaata aattaataaa ctgggatgct ataaatgcta
    13381 ggactatata tccacccata tcactcatac tactacccat catagatact aaatctttgt
    13441 cactttttat cttcttactt acttttccat acactaatcc tggagttaaa aatataatag
    13501 tgattatagg aactatacct ttcattaaag gtgcattact agatagtata gaaccagtct
    13561 ctggatcttt cataaatgca ttgtcaccta tacataaggc aactatcact actactacaa
    13621 ctaacaatga taataaagcg tattttatac ccttattttc aagttcactc aattcctgta
    13681 tatccttatc atttgttgaa tcattatatt ttgtaaaacg aggagctata aatttttcag
    13741 ttacaaatgc tcctgcaaaa gttaaaacta tcgctgatat aaaagcaaaa taaagattca
    13801 ttgctggatt gccttggtaa gatgggtcca tcatttgagc tgctggtatt gtaaatccag
    13861 ctaacaatac atctgtagta ctaagcataa catttgctgc aaatccccct gcaacacctg
    13921 caaaagctgc aaacatacct acaagaggat gtcttccaat tcctaaaaac acaagtgctg
    13981 caagtggtgg taatattata aatcctacat ctgctgctgt atgtgacaac atacctgcaa
    14041 atacgattgc tacagttaca agtttttgtg gaactttact tatacccttt ttcattaaca
    14101 cttccataaa tccagatttc tcagcaacac ctgctccaag cattgtaacc agtactaatc
    14161 ctaaaggagc aaatgactga aagtttgtaa ctatgctacc taaatatgat tgaatctgct
    14221 ctttctctag tagatttact acctttacaa cttcttttgt agaaggatgt atttcttcta
    14281 ctcctaaatt agatataact gctgacaata ttacaacaat tatacatagt aataaaaata
    14341 tggttactgg gtctggaagt ttatttcctg ctctctctac aacatttagt gccctcatca
    14401 ataatccact atctttgttt ttttcctgtg aattaatttt cctcaaacta ataccccctc
    14461 ataaacttta tctccagaat gctctatctt aaattttatt ttttaattta tccatataat
    14521 ttataacaga ctgacatagt acatttgtac ctaccaatat agaattttta tcagctttaa
    14581 atttaggatt atgccactga tatatacaat cttgttcttt gttgcctacc cctaaccagt
    14641 aaaaaaatcc tggggctttt tccatgaata tagaaaaatc ttcccctcct gtagaaggta
    14701 ttggatcaac tatagcatct tctgaataca aatcacacac agacttacaa gctattgcgt
    14761 acatctcttt actatttata acagctggta aatcaaataa ataatctagc tttgcagttg
    14821 cttgatatcc acgagctatc tcacaaacta tattttctaa attttctgat attttctttc
    14881 gtacttcatt actaaagctt ctacatgtgc ctgtcatatc taccttttct gaaattacat
    14941 tatttgcagt ccctccattt atagtaccta ttgatataac agctgattct aatggactta
    15001 tgtttctgct tactatggtt tgaataccca ttataattgc acttgatgct actatagggt
    15061 ctattgttct gttgggtata cctccgtgac ctccaaatcc atttacttct atcttaatag
    15121 tgtctactgc tgccattaac ccacctaact taacacctat ttttccacaa ggtatatctg
    15181 gatgattatg aagtccaaat atagcatcaa cttttggatt ttccattact cctcgttcca
    15241 ctagcatctt cgcaccttta ttgacttctt cggctggttg aaatataaac tttacatttc
    15301 catgaagact atctctaata tgagacaata catatgcaca tcctacaagg caagacgtgt
    15361 gtatatcatg cccacatgca tgcattatac catcatatct ggatttatac ttaatatcaa
    15421 cctcttcata tattggcaat gcatcaatat ctcctcttag tgctactgtg ggaccatcat
    15481 acttcccttt taataatcca acaactcctg tttccattcc taaatctaat atttctatgt
    15541 ctaaactcac taataaatct tttatagctt ttgtagtcct aaattcttta aaacttaatt
    15601 cggggtgcat atgaatatct gtaaaaaaat ctaaaatatc ttctttatac ttttctgaga
    15661 ttaaaataag ttcatgcata ctaacacctc tccaaaattt tattttttta ttttttataa
    15721 tttaactaca aattatataa gttaataatc tttataaaga tattttttat aaattt
//
LOCUS       DAMJYX010000079        15681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJYX010000079 DAMJYX010000000
VERSION     DAMJYX010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.080"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            121..1338
                     /locus_tag="PC479_002643"
     CDS             121..1338
                     /locus_tag="PC479_002643"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2805178.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1433..1813
                     /locus_tag="PC479_002644"
     CDS             1433..1813
                     /locus_tag="PC479_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2805179.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2097..2579
                     /locus_tag="PC479_002645"
     CDS             2097..2579
                     /locus_tag="PC479_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2805180.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2600..3415
                     /locus_tag="PC479_002646"
     CDS             2600..3415
                     /locus_tag="PC479_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2805181.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3466..5016
                     /locus_tag="PC479_002647"
     CDS             3466..5016
                     /locus_tag="PC479_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2805182.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5113..6465
                     /locus_tag="PC479_002648"
     CDS             5113..6465
                     /locus_tag="PC479_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2805183.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6596..7354
                     /locus_tag="PC479_002649"
     CDS             6596..7354
                     /locus_tag="PC479_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805184.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7401..8120
                     /locus_tag="PC479_002650"
     CDS             7401..8120
                     /locus_tag="PC479_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2805185.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8244..9644)
                     /locus_tag="PC479_002651"
     CDS             complement(8244..9644)
                     /locus_tag="PC479_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2805186.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9648..10556)
                     /locus_tag="PC479_002652"
     CDS             complement(9648..10556)
                     /locus_tag="PC479_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2805187.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10817..11464
                     /locus_tag="PC479_002653"
     CDS             10817..11464
                     /locus_tag="PC479_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2805188.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11789..14428
                     /locus_tag="PC479_002654"
     CDS             11789..14428
                     /locus_tag="PC479_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2805189.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14486..15589)
                     /locus_tag="PC479_002655"
     CDS             complement(14486..15589)
                     /locus_tag="PC479_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2805190.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
BASE COUNT         5906 a         1896 c         2741 g         5138 t
ORIGIN      
        1 gttttttgtg attaattaaa aaattataaa aatcgtttac atgttgattt ttaaaaaaaa
       61 aagatataga attatataga aagtaataaa caaaaattgg atttacggga gtggaatgaa
      121 atgatgaatg ttaccttaac agatgtgaaa gaggctagag agaccattaa gaacatagta
      181 aaaagaactg acttacttga aagtgttaaa cttagcgaaa aaacaggagc aaatgtgttc
      241 tataaatgtg agaatcttca aaaaacaggt tcttttaaat taagaggagc ttgtaacaaa
      301 atagcgagtt taacagatga agaaaaggca aatggagtta tagcttcaag tgcagggaat
      361 catgctcaag gagttgcatt gggagcaaaa atgactggta taaaatctac aatagtaatg
      421 ccagcaactg cgcctttagc aaaagttagt gcaactaaag gttatggagc agaagtagtt
      481 ttaaatggtg cggtttatga tgatgcatat gcaaaagctg ttgaaataca aaaagaaaca
      541 ggagcaactt tcttacatcc attcaatgat aagtatgtta tagcaggaca aggaactata
      601 tctcttgaaa ttttcgaaca attagacaat aaagttgata ctatactatg cccagttggt
      661 ggaggaggaa tcatctctgg tgttgctgtt gcagcaaaag cattaaatcc taatgtaaaa
      721 atagtcggtg ttcaaactgc aaatatacct tcaatgaaag agtcaataaa aaatggaaaa
      781 gtaacaacag catttaatga tactacaata gctgatggta tagctgttaa aactccagga
      841 gatttaactt ttgaaataat aaatgaattg gttgatgaaa tagtagtggt tgaagagaca
      901 gaaattgcag aatcaatatt atttatgatg gaaagtcaaa aaatagtttc agaaggtgct
      961 ggtgctgtat gtacagctgc tatattaagt ggtaagtatg taccagcaaa agatgaaaat
     1021 gtagtttgta taatatctgg tggtaatata gacataaata cgttatatag aataataggt
     1081 gttgctttag ctaaagaagg aagaagatat tcatttagta caattatgga agataagcca
     1141 ggaaactttg ctgagttaac tagaataata agtgagaatg gtggaaacat tttaagtgct
     1201 aatcaaggta aattatcagc aggggaagct ctaggaaaac aaagtgcgga atttatatta
     1261 gagacaatag attatgacca tatagctaga ataaagaaag ctatagaaga aaaaggtttt
     1321 aaaattatag aattataata aaaagtgtat ataatataca agaaaaatag tataatagtg
     1381 tatgaagata cattttatgt gtattagatt aacgaattag gaggatttta aaatgaaaca
     1441 tgaagtaata catactaatg atgcaccagc agctttagga ccatattctc aagctataaa
     1501 agctggaaac ttgttatttg tatctggaca agttcctctt gtaccagaga ctatggaagt
     1561 agttgaagga gatgtacaag ctcaaactgc tcaatcttta aaaaatctta aagctatatt
     1621 agctgaaagt ggagctgatt tttcaaatgt agttaagact acagtattta taaaagatat
     1681 gaatgaattt ggggctataa acgaagttta tgctgaatat tttggagaaa ataaaccagc
     1741 aagagcttgt gtagaagttg ctagattacc aaaagacgtt aaagttgaaa tagaagtaat
     1801 agctgtatta taaaaataca atatttttaa tttgaaaagg ctgaaaaact atattctagt
     1861 gattcagcct ttttataaaa aaataaatca caataaagaa catgataaat attgtcgaat
     1921 taaaaaataa aattaaaata aatattgcaa tatttttgag aaggttttat aatgagtata
     1981 taatttgaaa cgtgtaacta ttaaattagg catgagttct tggatatttt tatcctgagg
     2041 attcatgcct tttttgtatt gaaattcaaa aatctattta ataaggagga agtactgtgt
     2101 taaatttttt caagaaaaat aaaagctata agttacatgc agtagtgagt ggtaacagca
     2161 ttaatattga gaaagtaaac gattctgtgt tttcaaaaaa gttaatggga gatggagttg
     2221 caattattcc taatagtaat gttgttgtag ccccttgtaa tggtaaagta actgtgctta
     2281 cagaatcaaa gcatgccttt ggtatggtct cagatgaggg tgttgaaata ttagtccaca
     2341 taggaattga tacagttagt ttacagggtg aggggtttaa aaatgaagtg tctcaaggag
     2401 atactgttaa aaaaggtagt ccaataataa gttttgaaag agaaaaaata aattctcagg
     2461 gaattgattg tacaacaata ataattgtat taaatcatag cgaattttca gaaataaatt
     2521 gcatggtaga aaatgaagtt gttgcagggc aagatactgt tattgaaatt atgaaataat
     2581 aggaagggat taattcaata tgagagtagt aaaaatcttt aataataatg cattatctac
     2641 agtaactaat gataggaaag aagctatttt actaggtctt ggtataggtt ttaataaacg
     2701 tccaggagat aaagttaatg aagataaaat agaaaagatt tattatgttc aagaccacat
     2761 gcaaacaaaa tttttagaat tattaaaaaa tgttagtcca gaagtgatgg atgcttcaca
     2821 acaaattata agtcttatac caggaaatga gggaaaattt aataataaag ggatattatc
     2881 tttagtggaa catattagtt ttgcaataga tagaatgaaa aataatgttt ttttacctaa
     2941 tttaatgctt ttggatataa agatgatgta ttcaaaagag tttgaattag gagtaaaagc
     3001 tttagaaatt atatatagag tatgtcatat ccatttgcct gaagatgaag caggttacat
     3061 agctttacat tttgtcaatt tacaatcaaa tgataatttg gcatatgata ctttaaaatt
     3121 tgtaaaaggt agtattgatt tgattaaaga atgttatgga ttagaattag acgaatcgag
     3181 tttaagtaca ttacgtttta gaactcatct taaatttctt gctcaaagaa tttttaagaa
     3241 tgaaatttgt caggatgata aaatgattga aatgtatgat tatttaatta acaatcatcc
     3301 taaaaataaa gaatatttag aaaagttaaa tgtatatatt gaaaaggagt ttaggtataa
     3361 attagataaa cctgaaaaaa tttatctact actacaccta acaaaaatct tataagaatg
     3421 agatgaatta gtaatagaaa tatataattt taggggggga taagattgaa agaaaaacta
     3481 cttggaggac tggaaacatt tgcaaaggca atggttcagc cattaatgta tttatcgagt
     3541 gcaggaatat taatggtggt aggtgtacta cttacaaatg atatgttatt aggagtactt
     3601 ccatttttta aatgggagcc aattcaaata tttggaagcc taatttataa ctgtataatg
     3661 gctattatta ataatcttgc tgttatattt gctataggta ttcctgctgc actagcaaaa
     3721 aaagagaagc acaaagcagc gttaatagga tttatgagtt atataatata ccttacagct
     3781 tccaacacta tgttaacatc actaggtcaa ctagcaaaag aaagcaagat gcttggactg
     3841 atagggactg gacaagctac tattttaggt attcaagttg tagatacaag tgtatttggt
     3901 ggaattattt tagggtgtat agctggatat gtatttaatc gtacaagcaa caaaacattt
     3961 aaaggagctt tacagatata ctcgggagca aattttagtt ttatatgcat gttctttgta
     4021 tccattttaa ttggagttgt gagtaacttt atatggccat tagctcaaca aggaatagct
     4081 tctcttgcaa atgtcatcaa agatacaggt acatttggtt tattcttata tggtttttta
     4141 gagagaatat tagtaccaac tggacttcat catttaatct atacaccatt tcagttttct
     4201 gatttaggag gagtgcttaa agttggtgca gatacagttt caggagccta tgctatagtt
     4261 atgacagaaa tgaatttacc agttgagcgt ttctcagata gtatatacta catggcaact
     4321 ggatttacaa agatgtttgg ttatataggt ataggagctg cctttattta tactgcatat
     4381 aagcaaaata agaagaaagt taaggcaacg attattccac taataattac ggcagtatta
     4441 gcctctgtaa cagaaccatt agactttatg tttgtattct gtgcaccatt tttattcttt
     4501 gtacatgcag ttatagctgg tctttttata gctttattaa aaatatttga tgttactgca
     4561 ttatgtggag ggaacttatt agtatcaatt ataatgaata ctacagtagg cgtagaaaaa
     4621 acacactggc cagtaatgat gatattagga cttgcacaaa ttattgtata ttttgtagta
     4681 tttagttttt taattaagaa atttaattat aaaactccag gtcgtgaaga tgagagtaca
     4741 gctacaggcg aagaacaatc aaaacaactt aatttagatg cagggataga aaatataatt
     4801 gatgggttag gcggaaaaga aaatattaat acagtagaaa attgcatcac tagattacgt
     4861 gttaatgtaa aagatgaaag taagattaat gaagatataa ttaatttaac gccaaatagc
     4921 gggattgtaa ggaaaggaaa agatatacaa ataatttttg gacttcatgt gcatgaggta
     4981 agacgtgcag tagaagattt tttagaagct tattaaagat atatatttat atagcaaggg
     5041 taaataacag ctttaagtgc tgtttactat ttattaaaga tacatataat ttatagagga
     5101 ggaataatta ttatgattat tactattgtt ggtggaggaa gcacatttac tccaggtatt
     5161 gttaagtcaa ttgcattaag agaaaaagag ttagagcttg aggaaattcg cttatttgac
     5221 attgataaag aacgccaaga taaagtagct gtagttgtca actggatact aaaagaagat
     5281 gttaaaacag atgttaaact aactgtaact aattgtgaaa aagaagcgta tacagatgct
     5341 acatttatat ttgcacaaat gcgtgttggt aaatatgaaa tgcgtgagca ggatgaaaaa
     5401 attccattga aatatggttg tgtaggtcaa gaaacttgtg gttgtggagg gatggcatac
     5461 ggactgcgta caatacaacc aatgataaag gttattgatg atgtagagaa atatgctaaa
     5521 aaagattatt ggatattaaa ttactctaat cctgctgcaa tcgtttctga agcttgtcgt
     5581 aagttaagac caaatgcaag gattataaat atctgtgata tgccaatagc tattatagat
     5641 atgatagcgg ggtcattaaa tatcaatgac gttcataata tacgctatga ttattttggt
     5701 ctaaatcatt ttggctggtt tacttcaata gattataaac atagagactt aatggaagag
     5761 attaaggaat acataaaaga aaataaaata ttattaccaa aatcttatat agaaaactta
     5821 aataaaagtc gtagtacagg aaatcgccat gctgtaagta gctggataca tgtttgggaa
     5881 tctgtttata caatgttaga aagcttccca aaatatctgc caaacactta tttaacatac
     5941 tatcttttaa gtaaagaaac agtggaacat tcaaacataa atcgtactcg tgcaaatgag
     6001 gttatggaaa gccgtgaaaa agacttattt gaaggtgtga gacattactt agaaactgga
     6061 gaaatttctg aaaaagcatt ttatgcaggt tcacatggag attggattag tgatttggca
     6121 gtttcaatta aaaatgatac acgtagtcgt tttttagtta taactgaaaa tcgtggagct
     6181 ataccaaaca tgccatatga tgcaatggta gaaattccag catatattgg taaagatggt
     6241 ccagaagtta ttgctcgagg tccaatacct ctatttcaac aaggattaat gatgcaacaa
     6301 cttaacagtg aaaaattgat agtagaagct tatatagaaa atagttatga aaaagcttta
     6361 caagcattca cattaaataa aactattcca agtatgaatg ttgctaaaga aattttggat
     6421 gaaatgatag aagttaataa ggaatactgg ccagaattaa gatagtagga taaaggaaag
     6481 ggtttttatt taataattag atatgttttc agagtctttt atcaaaatgt attcacaaac
     6541 atatggcaat atattaaaat aaagagtgat atttttagaa gaatggggtg atgcaatgaa
     6601 cattataaat aaaatttttt gggaagaaga taggattggc atgattacaa ataatttaga
     6661 ggcagataca catagccact gtatgttaca actgttctta gggatagaag acagcattga
     6721 aataacagtc aatgaaaaat tagttaaatg taattgtatc atagtagata aaaatatctc
     6781 tcattcattt tcagcaagaa aaaaagttta ttactctgct attattgaac ctacatctat
     6841 atatgcagaa cagcttacct caaaaatgaa tgattttggc tactggatat gtgacaatga
     6901 tggattagag aaattacgtc aacaaggaac ttttttgata gataactcta gtaaagaaca
     6961 atatttaaga tttatggaaa tgcttaataa ctatttgaat atacctataa ctctcaaaca
     7021 ttatgatgac agaattactg aattgctcaa tttattacgt acttgtaatt gtgataatca
     7081 tactatttca agttttgcag ataaagtgtc cctatcagca agtagattat cacatctgtt
     7141 taaagaacaa atagggatac ctttaaaaag ctatatctta tttcatcaaa tggagtgtgc
     7201 ttttagagaa ttactttctg ggaaaaatgt taccgaagct tctatgactg ctggattcga
     7261 tactccatca cactttgcag ggacagttaa gcgaatgatg ggaatgcctg tttctttatc
     7321 tttgaaagat agcgaatttt tgaaagtata ttaaatattc tcgttatata atgaagttgt
     7381 actaagaaag gggctttgtt atgaataaag aatatttaga ggaaacaaaa atgttaaatt
     7441 ataatgaacc acaacttaag cttttggtat catcaaagaa ttggttggaa ttagatgatt
     7501 ttcataaaat aaaatctatt tatgagtttg tacagaatga tattttattt ggatataatg
     7561 cttctgatat gttaagcgca acacaggtat taaatgatgg tatgggacaa tgcaatacaa
     7621 aagctactct tttaatggca ttattacgtg cagtgaatat cccttgtagg cttcatgctt
     7681 ttgatgtaac aaaagatttt caacgaggag ctacatccaa attgatttca ctattagcac
     7741 caaaatatat tcttcatact tgggtagagg tattttatca agataggtgg attgctcttg
     7801 agggtgtcat aactgataaa aaatatttag aagcaattca gaagaaattt tttaatcatg
     7861 gtggaacatt taaaaagtat gcaatagcta caaatgattt gaaaaataca tctattgatt
     7921 gggatggtaa agatactttt atccaaaaag aagctatcgt gtatgattat ggtatttttc
     7981 catcaccaga cgtatttttt tctactcatt cacaacatat gtcaaaatta aaaaatttca
     8041 tatatgtaca tcttatcaga aaaataatga caaaaaatgt atgtaaggct agaaacaatt
     8101 atattgataa aaacgaatga gtgtttaaga ggtaactata taggaaatat atagaataat
     8161 taaaactgta ctggttaaaa tgaataaaaa ggagttatca cagagcaaac ttaaattgtt
     8221 cataggataa ctccttttct gtattaactt ctaataattt gatgtctttt tataaaacat
     8281 atagatacaa ttaaaccagt aaatgcaaga gtagctgcta ttgaaaatgc aacatctatt
     8341 ccatgaatgt tagacagcat gtttgaactc atatgagctt tcttagtagc acttgtcatt
     8401 atagtaataa gtatagcaga acctatagct ccagatatct gacggagtgt gttattaatt
     8461 gctgtggcat gagatataag ttctctatct aatgttttaa ttccccatgt agttaatggc
     8521 attaaaacca ttgaaatacc aatcatacga atacaataca tcaatgacac atgaattaaa
     8581 gatgtatctc tgcctaaaaa agcgaaggat aaagttccta aaaacaaaca tcctgttcct
     8641 aatattgata gagcatgagg tccatattta tctaaatgac gtcctgcaat tggattgagt
     8701 acaaccatca atatagcacc aggtaacatt aatgaacctg aagataatgc agaaaatcct
     8761 cttacagatt gaacgtacag aggaatcatt aatgtagcag acatcattga ggcatataca
     8821 atcataatta atattgtaga aataagaaat ggtttatttt taaaaaccct caattctaaa
     8881 aaaggttctt tagattttaa ttgacgcaat gtaaataaaa ttaatgacat aattcctatt
     8941 aatattggta aatatgtggc aatattagtc cacccataat taccttgatt agtaaatcca
     9001 attaataaac caccaaaacc tagagatgat agtactattg aaggtatatc taattttagt
     9061 ttttgagttt ccccaacatt tcttaataga ataaatgata gtataacatc taatatagca
     9121 ataggggaaa gaaacagaaa taaactatgc caaccaaatg aatctactaa ccatcctgat
     9181 aaagttggac ctattgctgg agcaaatcct actgtaatac caactaaact catagctgca
     9241 ccacgttttt cctcagggca taaatagagt ataataactt gtaaaagtgg cattagagaa
     9301 ccagaaccca ttgcctgcaa cacacgagca atcaacatag ttgaaaaatt attagaaaaa
     9361 agtgcaataa tacatcctat agaaaagata gacattgatg taataaataa ctttctagta
     9421 ctaaatcgat ttattaaata agctgttgtg ggtacaataa tacctgcaat aagtagataa
     9481 attgttgtta accactgacc aatatcagca gtaattttaa aatctgacat tatattaggt
     9541 aatgctgttg acaatagtgt ttgactgaga gaacttataa aagcaccagt cattacaatt
     9601 gctattatta aatttccttt tttattgtta ctttgggttt ccataattta taacgtctcc
     9661 ttttttaatt gatttttaat gctgttcaat aaaagtactt ttggtcttaa agtgtcttct
     9721 ttttctaaat atgcaatcat tgcatccata attaaagaat catattgttg attttttcct
     9781 tcgacgtata atttttttat tgcttctagt aatttaggat tttcaaggag agaagtatca
     9841 gttttgatta gttcttctat atcttgtatc gttttaaaaa atagcgattt attatgaagg
     9901 gaaaagtaaa cttcatcttc ttgtttatgt aaaaacattg atttttcaat taaaggattt
     9961 agattatcca tattttctac aactaaatct agaatttgta tagtaaattt ggcaataaag
    10021 tatacattta tttctctttt ttgtacatga ataatgtata taaactgatg aattagagca
    10081 tccaataaga aaactaaatc aaatataatt ttttctattt tatcaccata gttatcaaat
    10141 aagcttagtt tgtattcatt caataagtta gctcttgatt ttgtttgcaa tttacttgta
    10201 gccttacgtt gctcttcaga aaagttttct cttatatagg tatttatggt agttctagaa
    10261 aaaatatttt tccatacagc aattatcttt ctgtttaact tttctgatga tggaatgtca
    10321 ttattttcat ttatggaatg tataatttct aaaaactttt catcataata attgattatt
    10381 tcgcagataa aactttcctt agtttcaaaa tgcttgtaaa aagtagcttt agacatttta
    10441 cactctttta caacatccga tattgaagta ttttgaaaac ctttttggtc aaaaattttt
    10501 tgtgcaatag ttataatgag cctttgtttt tctgtcactt tattatctcc tttcacgcaa
    10561 aaacgtttag tactatatag tacctacaag ttttgataaa tatattatac atcatttgga
    10621 aaaaaaatca atatatagac tgtaatatga aatttttgaa aaatttgttg ttatttttag
    10681 tcgtataaaa aagaaactta agagtttctt aagaattgtt taaagaagtt gcaataaatt
    10741 agtggtatga ttatagtatg aaagagaatg tcttgaaatt tttctgtatc aaattccaat
    10801 tatggaggtg tagtatatga aaaagaaaat ttgtgtagca ataatttttg ctctatttat
    10861 agtttttgta gaaatgttta ttgatagctc tagcgaagga agtaaaatta tgacagctga
    10921 attaagtaaa tacaggaaag gcgtaaatgt attatcaata aacaatatta tagtagcgaa
    10981 taaaaaatat agtttgccca aagattattc tcctcaggaa agcagtgaag cgagagatgc
    11041 tttttataaa atgaataaag atgctcaaaa gtcaggatta aatttaaaag cttttagtac
    11101 atatagaagt tatgaatatc aagatagatt atttaagtca tatgtaaaag aacatggaga
    11161 aaaagaagca aatagatttt cagctaaacc tggagagagt gagcatcaaa ctggacttgc
    11221 ttttgatatt ggaggagatg accagtcttg ttgggcaaat aaaaagttca acaatacaaa
    11281 agaagctaag tggttgtatg aaaatgcata taaatatggt tttatactta gatatccaga
    11341 aggtaaagag aatataacag gatatatgta tgaatcttgg cactatagat atgttggaac
    11401 tgaacatagc aaaaactttg caatgaataa tttgacttta gaagaatatt tacatataaa
    11461 ttaagagatg gtttgttaaa attaggttaa aataaaaaat aacctagact attagctgtg
    11521 taaagtgata taatgtaaat acaatttaag ataactactc gaaggggagt agcttacacg
    11581 aaagtggtaa ttaatcgtca agacagtaga aatactcggt taattagcaa gggattcctt
    11641 gatagcaaga ccttcaaaat aagcattagg tatgtttgtt ttggggtctt tttttattca
    11701 aggaattttt atttaaaaaa atttaaattt caagtataca taagagcccc caaacataaa
    11761 ataaatttat tgaagaggag ggcaacaaat gtcttacaaa agtacttatc aagaagtaat
    11821 taaaaattta gactcaagtt tatctggttt gagtaaagaa agagcagaag agttgttaga
    11881 aaaaaatgga gctaatgaat taaaagaagc agataaggtt cctacttata agcttttcct
    11941 agaaagtttt aaagacccat tagtaataat tctattgata gctgctttag tacaaatatt
    12001 tttaggtgaa acagtggagt ctataataat ttttgcagtt attattataa actctgttct
    12061 gggtgttgta caaacaaaaa aagcagaaag ttcattagaa agtttgaaaa atttatctgc
    12121 accaaacgca aaggtaataa gagacaataa aaaaatgaca attccagcaa aaaatctagt
    12181 agttggagat attgtattct tagaagctgg cgattacata ccagcagatg gaaggcttat
    12241 agaagcacaa tctctcaagg ttgtagaagg gatgttaact ggagaatcag aaccagtttt
    12301 aaagcatata gaaaaaataa gtgaagatgt aagtttaggt gaccagaaaa atatggtatt
    12361 tagtggtgct gtagttgttt atggtagagg ttcttttgta gtgactggaa caggtatgaa
    12421 tactgagatg ggtaaaatag ctgggctttt agaaagtgct gaaaataaac ttacaccatt
    12481 gcaacaaaaa atagaggatt ttagtaaaaa acttggtgta ggtattgtaa tacttgctct
    12541 tttaatattt ataattcaag tagcaagaaa ttactttatg gctggaaatg gagaaatgac
    12601 acaaattatt ttagattcat tcatgttctc tgtagcagta gcagtagcag ctataccaga
    12661 agctctatca tcaatagtca ctatagtatt ggctgttgga acaaatagta tggcaaaaaa
    12721 acaagccata ataagaaaat taccagcagt agaaacttta ggttcaacaa gtgtaatatg
    12781 tactgataaa acaggtactc ttacacaaaa taaaatgaca gtagttgatt attatatgta
    12841 tggaactaat aatgatgagt tagataaaga aaaagtaaac tattcttatc atgctaaatt
    12901 attgactatg gtttctacat tgtgtaatga ctctcatata actagtgatg gtaaagaagt
    12961 tggtgaccca acagaagtgg ctttaataaa ttattcaagt aaaaatgatt tagattatga
    13021 taaattaaga agtaagtata tcagaataaa tgaaatacca tttgactcag acagaaagct
    13081 tatgtctact gtaaatagta tatatggaga ttatataatg tttacaaaag gtgctccaga
    13141 tattgtgttt agtagatgta aatatgcttt gaaaaatggt tcaaaagtag atataacaga
    13201 tgagataata gaagaatata aacataaaaa tgaagagttc tcaaatagag cacttagagt
    13261 attagctttt gcaataaaag atgtcccaga agaagatttt ataccttcaa ttgatgacga
    13321 acatgacatg acattggttg gaataatggc aatgatagac ccaccaagag aagaagtaat
    13381 ggatgcagta aaagaggcaa agagtgctgg tataaaaact gttatgataa ctggtgacca
    13441 taaaacaaca gcagcagcaa ttgcaaaaga gattggtata atggaagaag gagacttggc
    13501 tcttactgga aaagagcttg atgctttgag tgaagaacaa ctatatgaaa aactagaaaa
    13561 tataagtgta tatgccagag tctcgccgga aaataaaatt cgtatagtaa aagcatggca
    13621 acataagaat aatataacag caatgactgg tgatggtgtt aatgatgcac ctgcattaaa
    13681 acaagcagat ataggaatcg gtatgggaag tggtactgat gtagcaaaag atgcttctgc
    13741 tatggtattg gttgatgata actttgcaag tatagtaaat gctgttgaag ttggtagaac
    13801 agtatacagt aatataataa aatcaattac ttatctattt gcaggaaact taggagctat
    13861 agtatccata gtatttgcag tgtttgcagg ttggtcaaat ccatttacag ctttacaact
    13921 tttgtttata aacttagtaa atgactcttt accagcaatt gcattagggt ttgaaaagtc
    13981 agaaaaaaat ataatgaata aacctcctag acacccggat gaaagtatac tagcaggagg
    14041 aacattgaag gcagttgcta taagaggttg tataataggt gtagttacaa taattgcaca
    14101 atatatagga ttacaagtat ctccagaact tggagtagca atggcatttt caacattgat
    14161 attagctaga ataatgcaaa cattacctgc acgttctaat aatcaaacag taataaaatt
    14221 aggttttttc agcaataaat acgttatagg agcagtttta gtctgtcttg ggctttactc
    14281 tttaacacta ataccagtgc taagaggaat attctctata ccaacaacgt ttggattgta
    14341 ccagttgggg atttgcctag ggttagcatt aatttcatca atattaatgg aacttaacaa
    14401 attaataaat agaagtgaag taaaataatc aaaaaattaa agggcatatt gattttaaca
    14461 atatgccttt tataaatagt ctaatttatt ctatattatc gattatttgg aatgctttta
    14521 taacttcagg taattctttt tcttcaggca ttctaaatct tataaaattt ttatcaagtc
    14581 cagtaaaatc acttccagat ataactctta tttttaattt agcaaactct tgttgtaaat
    14641 ctatgtcctt gtttttatgt tcaactgtca taattgatac agtatcacta gtttcagcta
    14701 tattaaggtt tttccatgga attaataatt gattttttat atcttttgtt ttttctttta
    14761 attcttctat aaattgaaca tcatctaata aatttgctgc aatactacga gatatttcag
    14821 acacttcgta aggagtagaa atctttttaa tagggctaac aagttgttct ggaagaactg
    14881 catatcctgc tcttagtcct gctaaaccaa atccttttga aaatgttttt aaagcaatta
    14941 cattatcata attatttaga agcttgacag ctgaattttc ttttggcatg tattcaccat
    15001 aagcttcatc taccataact gctatatcat atttagctgc ttcccttact atattttcaa
    15061 ttgatgataa aggtataatt tgtcctgtag gattgtttgg attgtctatg taaattactt
    15121 tatattctgg atttattttt tcaataaatt ctttttcatt aaacttgaaa ttatcctctt
    15181 tctttaaaag aacataatca taagttgcac catgcatttt tatatctgtc tcatattctg
    15241 agaactgagg tgaatatcct aaaactttat ctcccttttc aataaatagt ctatttaaaa
    15301 ggtaaataac atgtatagaa ccatctgcca aacaaattct gtcagtatct aatgctataa
    15361 aatctttcca ataatcgact atattatctt ttaaagcaat tgaatgagga tatcctctta
    15421 tcatatcaaa ttttaaagca ttaaaagctt ctacagcttt gtttgagtaa gacacggtat
    15481 ttataccttc accacagtct atctcgaata catcttctga atgtccagat tctattgcat
    15541 aacttttttt caactgcatt tcaatagctt tgttaatttt tagtttcata ctttatcact
    15601 ccttatttag tcaataataa atgttataaa agttgctaga tttaaaatat tatgaaaata
    15661 tataatagta aaaatgaata t
//
LOCUS       DAMJYX010000080        15598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJYX010000080 DAMJYX010000000
VERSION     DAMJYX010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.081"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(41..901)
                     /locus_tag="PC479_002656"
     CDS             complement(41..901)
                     /locus_tag="PC479_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2805191.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEIEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1218..2546)
                     /locus_tag="PC479_002657"
     CDS             complement(1218..2546)
                     /locus_tag="PC479_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2805192.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2694..3023)
                     /locus_tag="PC479_002658"
     CDS             complement(2694..3023)
                     /locus_tag="PC479_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2805193.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3047..4336)
                     /locus_tag="PC479_002659"
     CDS             complement(3047..4336)
                     /locus_tag="PC479_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2805194.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4352..4678)
                     /locus_tag="PC479_002660"
     CDS             complement(4352..4678)
                     /locus_tag="PC479_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2805195.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4813..5442)
                     /locus_tag="PC479_002661"
     CDS             complement(4813..5442)
                     /locus_tag="PC479_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805196.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5657..8314)
                     /locus_tag="PC479_002662"
     CDS             complement(5657..8314)
                     /locus_tag="PC479_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805197.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8673..9617)
                     /locus_tag="PC479_002663"
     CDS             complement(8673..9617)
                     /locus_tag="PC479_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805198.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9610..10635)
                     /locus_tag="PC479_002664"
     CDS             complement(9610..10635)
                     /locus_tag="PC479_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805199.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10710..12278)
                     /locus_tag="PC479_002665"
     CDS             complement(10710..12278)
                     /locus_tag="PC479_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2805200.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12391..13302)
                     /locus_tag="PC479_002666"
     CDS             complement(12391..13302)
                     /locus_tag="PC479_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805201.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13286..14272)
                     /locus_tag="PC479_002667"
     CDS             complement(13286..14272)
                     /locus_tag="PC479_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805202.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14632..15033
                     /locus_tag="PC479_002668"
     CDS             14632..15033
                     /locus_tag="PC479_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2805203.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15036..15434
                     /locus_tag="PC479_002669"
     CDS             15036..15434
                     /locus_tag="PC479_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2805204.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4918 a         2722 c         1727 g         6231 t
ORIGIN      
        1 tattatataa taagtatttt taggattctg ttacataata ttatttaaat attcttctat
       61 atttctcttc ttcttcttca gtaaacacat taaatacaac ttttaaattt ttttcaggat
      121 tttcttttag ccatagtcta actctactta cagcaagatt agctgcctct ttttttggat
      181 aaccaaatac acctgtagaa attccacaaa aaacaatatt ttttatttca tcaatttctt
      241 ttattgtatt taaacatgat ttataacaat gaagcagttg cttttcttgc tctttacttg
      301 gctgaccacc agatacaata ggtccaacag tatgtactac aaattttgca ggtaaacaat
      361 aacctctagt tatcttagca tctccagtat attctaaatg cccttgtttt tttattattt
      421 tgtcacaatc ttctctaagt ctaggacctg cgcatgaatg tatctcatta tccacgcata
      481 gatgtaatgg ttgtaaacac cctaataact tgttatttgc agcatttact attgcatctg
      541 ctcttaaatt cgttatatta cctctccata tggcaatccc ttcctctatt tcttctatat
      601 catttacatc tactaactct ctttcttcac attcaatcaa caataaatta tgtaacatat
      661 taagtatctc tggagcaatt tcttttggtg gatatacatt caaaagtcct ctaagtatat
      721 ctctaggctt aatttcatca tttgataaat tgtatatttt agaaggaact tcttttgaaa
      781 aatatgcaat taaagtgttt atattttttc ttctttgttc gtcagttaga ggcttgatag
      841 ttttatcaaa atcctcaaat aaatttacat catcagcata atctctccac ttcatagcca
      901 tctctccatt cttgtatttt agacttaaaa ataattatgt atttatgtac atatttattt
      961 atttctataa acacctacat aaatcctgtt aaaagtgtaa attttttatt atgcaacttc
     1021 tctggttatt tctattgatt gatagtaaat tattgtacct aaatttaatc caactaacaa
     1081 gtaactaaac ctactttcct aactgcaaaa tacactatgt ttgccacttg tatctcattt
     1141 gtaaataaat aaagtcccta caaatacatt aactaggcta tgtactaaac tgtaggaact
     1201 ttaaaaatat attattctta attaagtttt ggccaataat ttttatttgc ttctattaaa
     1261 tcatctagta attcttttgc aactgttgca ctaggaactg tcttagatag cataagtgct
     1321 tgccacaatt tattataaga cttctcaatc caagcatcaa ctactaattt ttcaactgct
     1381 aattgttgtt ctattaaacc tttttggaat gttggtattt tccctattga taatggttca
     1441 tacccgtctt ttccaactat acatggaatt tcaaccatag catcatcttg tatattttct
     1501 atagctccat tattttgaac tatcaataac atacgctcct gtgtattaaa agctagagct
     1561 gtagctaaat ccactataaa ctctgcatgt attcctatct taagcccact gcccttagaa
     1621 tgtccttgct tttctatcaa tgcacatgaa ccaaaaactt ctttttctct tccatcaata
     1681 acttcattgg cacgagtata atttacatca gaatgattta caacataatc tggaaataga
     1741 tagtacttta agtaactact tggtattgta gttaaatctg tttttactat ttctttagta
     1801 aataaattag tttcaaacca actatctttc ttgtcatttt tcattccttc ttcaccattt
     1861 ataaaaccat attcttttat atgttctact aattttggta aaagttcatt tcctttttta
     1921 tctttaattg aagtatacca accaaagtgg tttaatccaa agtatcttat atccatatgt
     1981 ttacgagatt tgagtcctgc aatccttgct atattatgtt ccatacctat tggcatatca
     2041 caaatattta ttaccctaga attaggtctc aaccttctaa cagcttccgc tattattgat
     2101 gctggattag aataatttaa catccaacaa tttggagaat acttttccat ataatctatg
     2161 ttttcaagaa caccttctat agacctcatt ccataagcta ttccacctgg tccacaagtt
     2221 tcttgaccaa ctaccccatg ttttagaggt atcttttcat ctagttctct catttcaagt
     2281 tttccaactc ttatatgagc taaacaaaaa tctacatctg tataagcctc tttagggcat
     2341 gttgttgcta aatactctat attagggtcc ttttccttta ttagtatttc acaagcttta
     2401 gctaccttat tttgtctttc ttcatcatta tcatataatt tgattgctct taatggtaac
     2461 ctatctaagt tatctagcaa cattaatatg atttctgggg tatatgtact tcctcctcca
     2521 gctattacta ctgaaaattt ttccataata atatctcctt ttctatttaa aaatttactg
     2581 tatttataaa taaatttata aatactagta ctttaaagta aaagggagtt catttattaa
     2641 atctattgat ttaataaact atagccaaac ttcaagcact atttacaaga aatttaattc
     2701 ttattaattc ttgggtccat catctcaaat atatctacaa gttcagatac taaagaatga
     2761 gcaaataatg catttgaata atggtcttca gagtgcacaa ctaaaaaatc agtcccacta
     2821 tcttcactat tcatagcatt tgatagtaat tctaaatgat gctttgatgc tgtttgtatt
     2881 ttctctgagg ctgaatttaa aaggtgtttt gctttattgt agtcaccttt ttttatttcc
     2941 ttaagagaca tcattacatc acttttagca tctcctgctt ctgatattat actaaatact
     3001 ttttcttcca tttcaggtac catatccttc tccttctaat gacttgttaa aaatcaaagc
     3061 tatcatcatc taatgttgta gctaattctg catttttatt taattcttcc ttctccatta
     3121 tcttaaagaa tggataatat atcaaggtca taagtattat acttgcaaat actaatacga
     3181 ttgccttcca atctaatgtc atcaaaaatg ctgcaacccc tggaggtaaa aagcctggtg
     3241 gttctgcaat aggtaatcct actaatccta cttttaatgc cataaaatta agtgctccaa
     3301 ccaatacagg tccaaataca aatggtacaa acatatatgg gttcataact attggtgttc
     3361 caaatattac cggctcattt attccaaata gtgatggtaa aatcacaact tttcccatac
     3421 tattaaaact cttacactta cttcttgaca taaggtacac taatcccaaa gttatccctg
     3481 aaccagttac ttgcaagaaa ccatagtaaa aacctattgt aaacacatat tttgggtctc
     3541 ctcctgctgc tattaacgtt gcattttctg ccatatatgt agctgctatt ggcatccata
     3601 tacttgaagt tattgctgct ccatgaagcc caaagaacca taataattga gttataaata
     3661 gtattatagt tactgcaaaa acattatcta cactgcttat agctggtgct aagaaattca
     3721 ttataatagc tggtaatggt tgacctgaga catttttagt tattgctgct attgctgtaa
     3781 ataacattat acatactatc attggagtta agtttctaaa acttttattt actacactag
     3841 gtacttcttt tggaaatcta attacgagat ttttatcatt cataaactta gtaatatcaa
     3901 ctactaaaag tgctactaat atacttacaa ataaaccttg tgcatctaaa taacttgtat
     3961 ctaatacacc atctactact tgtccacata caacaagttg tgcaacaact gctgtcatac
     4021 ctatttctac atgattaaga ttatattttt tagctagtgc ctgagctata aaaaatgctg
     4081 aatatataga taataaactc atagtaaatt tattagggat aagtaatgta tccatatttt
     4141 cagataataa tgtttttgca cttccttcag gtaacatatt gtttaatgcc ataaataaca
     4201 tacttaaaga accaacaatt attattgggg taatgcttat cataccttct tttatagctt
     4261 gaagatgcct ttgtttttca atcttttgtg ctattggaag aagaaatctt cttaaattat
     4321 tttgaagtgc ttccattttt attcccccca attatctatt tttatataat tcgattgccg
     4381 acttcataac agaaggagca tccatacgac cataatgctt agtatctatt aaaccaactg
     4441 ctattttgta attcttacaa gcttcaacta catcatcttc cttaaaccta atttgtggtg
     4501 ctacaagtac tacatcggta tcattagctt caatgtatga ttttaaattt tcaaatgatg
     4561 ttgcctctat atttatagtt ttagttttta acttttcatg ctcactacaa aaacttttca
     4621 tcttttccac aagtacacct gttgaagctc catttgcaca tactaataaa atattcataa
     4681 ttaccctcct acaatatcta acctctttta ttttctaatt ttttatcagc caattcttac
     4741 tctaattcct atcagttttt actttaattc ttattatttt attttaattc ttattaatat
     4801 ttactctaat tcttagtact gccaatgatt aaacttgaaa gccgtatcta taacttcttc
     4861 ttgcttatat ttttctaata tttttaagta tctttcttta taatcattat ctacttcttt
     4921 ttctggtatt actatactag cttcatttaa aaagtggaat gaatcccttc ccatttctaa
     4981 tcctctatat gtatgcttgt caagtgccac gtccataact tctggaactt tacccattgc
     5041 aaatcctttt ataattatat tttttaataa atcacttgat ctatctttct cacatgcaca
     5101 aagatatctt attgcatgta tgaaaaataa aggtctgtct ccatctgcat atgaatattc
     5161 ctttcttact tgatttaaat tatttatgat tattacagct gatgggtccc ccattcctat
     5221 atcttcaact gatattgtaa gtatccttct ccatagctta tcttctaatt ctggtgatga
     5281 tatatatagt tcataagcaa attcacaagc ttcttccacc atatttcttc ttatagcttt
     5341 ttgaaatgac gatattattt catctgcact aaatccgttt cttgtagtca ttcttgccca
     5401 tgggtcattt gcctttttat ctcttaatat tttatcttgc atattattta ctccttttta
     5461 tttaaatttt aattatttat attactcatt taataatata agcaagtatt atgccagttt
     5521 ttttagacac tttaaaacac tgttatttca atcattttta tcatcaaaac ttagatactt
     5581 tgtctaagta tatttatatg tgtctaatat taatattatt ttagactaaa aaaaattacc
     5641 aaataagttt ttatactcat tggtaatctt tttcgattat ttctgcatat tctaatatta
     5701 ataatatttg atagcactct ttttcattaa tttctatatt atatttttta tctaattgag
     5761 atactatatc ttttattttg cttaggctct tgtgataatc aagtgttttt tctttatcaa
     5821 gcttttcaat ttctctagta tttccaatca atccatctac catgaaagct aaatgcatca
     5881 taagtccaat tactttatcg tatttctttt ctatattaaa tactaaaaac aattcatcag
     5941 acacatattt tatatcttta aatatcctat tataattaga gctttttaag gatgtcttta
     6001 aaccttcaac tacatcatca taacctatac ttatctttat aatatttttt aatttattga
     6061 tattttcatc cttatctata tcttttttgc taatatagtc aattcctcta atattaattc
     6121 tttgttcatt gattactgca acaatattac tactttcttt aagtttcgta atatttaaac
     6181 ttaaatcttt ttctgtttta tatgggacag atataatatc tattccatct aagtacctac
     6241 tcatcttttc ttctaatatt tttcttgttt tattatctac accttctgaa acatgtgatg
     6301 ctataataat tgttttagac agattttctt tattttgaat ttcattagtt ccaaaatagt
     6361 actttctcgt gttagttata gagttgtata cgtcatttaa aggtaatccc atattggcct
     6421 tctgaactgc ttctaacaca gttaatgttg tcaccatagg aattactctt actccacact
     6481 ttttttcttt ctttataatt tcatcaaaag agtttaatga acccatatct gtaaatagta
     6541 atacatcttt attgtctata ttattaatta aatttttaaa atctacaata acttcatcat
     6601 agcttttatc taacttcata tcgaaagaaa tagcactgca ctttccaata atatcaccaa
     6661 ctgcttttac catactactt gctgtagttt ctccatgcat agcaactata atattaatgt
     6721 tattcactat tttattttgt tcatcttgtt tagcttgtac tatgaataat gtaatatatg
     6781 ctacagtttc tatagataaa aataaatcaa attcattttc aattattcca actattttta
     6841 gtgctgtttt aaattcactt ctgttatttc ttctaatttg attcaaatct atgttgtttg
     6901 tagagctact tttatccaat acgcttttta tataaattgc cattgactta aatattgatt
     6961 cacttatgtc tatttcttca tcttctttaa taatttgtct tattgattca cacatgcttt
     7021 taaacttaac attattaaat agagaagcat ctgctatatt catttctaca gatttattaa
     7081 gattagtatt tactgcatta ataatattta tatcattgta gttaataact tcttcttctc
     7141 ctaatgacgt aaatacaaaa tattctattc catgtgttat aacttcatct gctttaatag
     7201 acttctctct ataatcatca ctcacttcat ttatagattt atcaaaatat tttttctcta
     7261 tataaactgg ttctttatta tctcttttat atcttaggta tccatttgca acagctaatt
     7321 ttatatcatt ttgaagttga cctacattat ttttacaatc ataaagtaat agtgatttaa
     7381 ttatttcttt atgtactttt attgaaatag aaatactttt tgattcttcc ttgaaaaaat
     7441 ttttgactaa ttccattctt tcaatcattg tcctatctct cagtgatggt atttctattt
     7501 tcattggtat tcttctattg aatgtatcta atagtgttga tgtcggattt tctgtagtag
     7561 cacatattat taatacctta gactctcttt ctttcgttgc atctcctagt cttttaaatg
     7621 ttcccttatc tataaaagta aacaacattt cttgaccttc tgctggtagt ctatgaattt
     7681 catctaaaaa tagtatccct ccatttgcaa tttccaaaag accttttctg gaagattctg
     7741 cacctgtata agctccttgt tctaccccaa aaatatgtcc cataagtaat tgtgcattat
     7801 tagcataatc agcacaatta aatgttacaa aaggagcttt attttttcta attccactat
     7861 ctatagaata atcgtacata tatctagcaa tcatactctt tcctactcct gtttctccat
     7921 agataatagt atgaagtcca tttgtaggat agagcattgc gaccttgcat ttttctatta
     7981 taggtcttaa actatgttct ttacctatta gtgtttcaaa actatctttt tttattttgt
     8041 tttctacttt tgttcctttt tttatgccaa gcaactcact ttgtgtatca agcaattcat
     8101 tcttgttaag gttaagattt gcagggatat aatacacagg tcttcctttt atcttcgtaa
     8161 ccataccctc attgaataat tggtttaaat atgtacttat attgtttctt gcaattcctg
     8221 tcttttctga taattggtta gctgtaatcc ctttttcatt ttccctatat gatgattcta
     8281 aaactttcat tactttatct tttttatata tcattacaag ctcccctaca ttatagtatt
     8341 ttattaacta aaacccctat acacacttta ttttttaata aactcttatt ctttaactaa
     8401 ggttataatt tatcaaactt tccttacttt aaattataga aaattattgt agaaaaaata
     8461 agattattgc tcattttttc tacattacta tttttaattt tatagtactt tcttaacaac
     8521 atactttact tgtttttaat tttattatat ttgaatttat tatatttgaa aagtagaaat
     8581 aaaaaaccct attttggagt agttttaaaa tttctccaaa aatagggttt tagtttgtat
     8641 ttcaaataaa tacctgttca agtattttta tactaaacaa cttttaaatt ttgttgatat
     8701 ttatttattt ctgatttaga agaatataca aaatgtccat ctgaaacatc aatccaactt
     8761 aatgactttt catttgaaca atcatatcca ttagaattat actctattct atttcttgac
     8821 ttagcatatc tagggtcagg aacaggaatt gcagaaagta gtgattttgt atatggatga
     8881 ataggattat tatacaattc atttgaatct gccaattcta ctaattttcc attatacata
     8941 actcctatac ggtcactaat atgtctcacc atagataaat catgagctat aaataaacaa
     9001 gttaaattct tttcttgttg caatttttta aataaattta ctatttgtgc ttgtatagac
     9061 acatccaaag aagctatagg ctcatcagct attataaatt ctgggtctac tgaaagagct
     9121 cgtgctatgc caatacgctg tctttgtcca cctgaaaaat cagatggata cttatttgca
     9181 taacttctat caagtcctac tagctttagc atctcatata ctttattcat tctatctgtt
     9241 ttatttttac agataccttg aatttttagt ggctctgaaa ttatatctgc aattgtcata
     9301 cgtggattta atgatgaagt agaatcttga aaaataatct gcatactttt gtttacatct
     9361 ttttttataa atttgtacgt ttttttatct gatatgcaat ttcctttgta aattacttct
     9421 ccaccagtag attcatgtag tcttattata gttttaccag ttgtagattt tcctgaaccc
     9481 gactctccta ccaatccaaa tatctcaccc ttatatatat caaaacttat atcatcaact
     9541 gcttttacag tagtactttt gtccaaatga aaatattgct ttaagttttt tatttccagt
     9601 attttttctc tattcattag aaatcaccct tccacaattt actctaactg gtacatctac
     9661 ttcaggtgca tctgggtgca ataaccaagt tgctgcacta tgtgtatcat taattttaaa
     9721 cattggtggc tctttttcat aatctatctt aagtgcattt ttattcctta ttgcaaaagc
     9781 atctcctttt ggtgggttca ataaatttgg aggcattcca ggtatattat ataaatagtc
     9841 atcctgcgaa tctaaagtag gtaatgaacc caacaacccc caagtatatg gatgtctagg
     9901 gtcataaaat atatcctcta cagttcctat ttctacaatc tttcctgcat acataactgc
     9961 tattctatca gctatagttg caactactcc taaatcatga gttataaata ttattgacaa
    10021 acctatttta tgttgtaaat cctttattaa atcaattatt tgagcttgta tagttacatc
    10081 taaagcagtt gtaggttcat cagcaatcaa tacatctgga ttacaagcaa gtgctatagc
    10141 tattactata cgttgtctca ttccacctga aaaatgatgt ggaaattgtt taaatctttt
    10201 ctcaggatta tcaattccaa ccaattcaat aagctctatg gctctctttt ttgcctcact
    10261 tttacttata ccttgatgaa tgattacagc ttctgcaatt tgctttccta ttgatatagt
    10321 tggattgaga gatgtcatag ggtcttgaaa tatcattgat atattctttc ctcttatatc
    10381 ttccatctct ttttcagact tcttaaccaa atcacttgat ttaagtaaaa cctctccatt
    10441 tttaatatat ccattgtatg ggagtatcct catcaaactc ttacacagta cagatttccc
    10501 acaaccagat tctccaacta tagccaatgt ctcacctttt tttagattaa aagatacatt
    10561 tcttacagcc tgaacttctc cttcttctac tttaaaactt acagatagat tattaacctc
    10621 caataaatgt tccaaaaacc atcaacccct atgctattca aaccttaggg atatattttt
    10681 atccctaagg tttctaatta atttcagtat tattcaattg tccagtcagc tatgttccag
    10741 aatataccta caccatgatg acctaaaact gtatcaggtg ttaatccttt tatgtttggt
    10801 tttccaacat atattgcatc tatataagca ataaatgtat aaggcatatc cttagtcatt
    10861 tctacttgga attgtttata taactttaat ttttcatctt tatcttctgt ttctcttgct
    10921 ttttgaagta ttttatctat agtaggatta gagtaagcac tgtaatttgc acctttatct
    10981 gttccaaata ctttatatgt atggtcatct gggtcaaatg gacttcccca acctattaaa
    11041 tgagcatctt gattagccca atctgtttca gttacaacaa cagctttagc atcaacacca
    11101 atttctttta actgttgtgc acatatttta gccatatcaa ctctaacttg gtcactctca
    11161 cctgctgtta tctcgaaagc taacttagta ccttcttttt catagattcc atcagaacct
    11221 aatttccaac ctagcttttc aatttcttgt ttagcttttt ctggattata ttcaaacttt
    11281 tctatatcag gattattata tggtcccatt tgtaaaggtg aatatgctgg tacaccatgt
    11341 cctaataata cactgtcaac tattgctttt ctatctattg catagcttaa agcatttggt
    11401 aatcctttag cttttttatc tttaaagaat ttagaattaa aattgtataa tatacctcta
    11461 tagtcggctg ttttcattat atttacttta aaattctttt cgtctttttc aaagttagac
    11521 atatctttag gagttatttg agctaggtct aattctcctg attttaattg cattgcctta
    11581 gccttatcat ctggaacaat cttaaagact actttatcta atcctggttc ttttacaaaa
    11641 taatcactat tcttaacaag tgttatactt tgacctttgt cccatttttc aagtttaaat
    11701 ggtccagtac ctattggttt ttgattaaat tcatcagtag ctatatcctt accttccaaa
    11761 gcatgttttg gtaatactcc tactgttaaa taatcaagca ttgctgtatt tggagctttt
    11821 aaagtaattt ttatagtatt atcattaacc acatcaattt ttgttatatc ttcataattt
    11881 gatgctatct cagaagcatt atctggattc attatagttt ctattgtaaa cttaacatca
    11941 tttgctgtaa atttttctcc atcatgccac ttaacatcat ctcttaaatt aaaagtatat
    12001 gtatttgttg cctcatcaaa tttccagtcc tttgctagac aaggaaccac cttattattt
    12061 tcatcatgag ctgttaatcc attaaatatt aacgaattta tttctccatg ttcatatagt
    12121 gcaggattta tacttgtata gtcattactt ccataaacaa gcacctttcc atctttaggc
    12181 gtatctgcct tcttatcttt gtctcctcca ctagaacaac ctgccattag actaagtatc
    12241 attactaaac ttaaaacttt taacttcttt aatttcatga tgctccccct tttgcttttt
    12301 ataaaatatt tttcaaagct ttgaccccat ttatttaaat agatatttca aatacctcag
    12361 tatttgctta tttaatactc aaatgctgat ttataaatta ctagattttt tattattatt
    12421 ctttcttata taattaccta tatttgttat acacaccaat gtggtaacca aaaatatacc
    12481 cggaatcagt ataatccacc atctatttga aagtaatgct tcctctgaca aagataacat
    12541 acttccccaa gaaacaattt ctattggtaa accaattcct aaaaagctaa gtgtcgattc
    12601 agtaccaata gctgcgccta tgtttgttac aaccatgaac attattgatg ccacaaaatt
    12661 tggaagtaaa tgttgcttta atacatagaa gaatcctcct cccatgctct tagctgctag
    12721 tatgtattcg ctatttttta tttgtctaac ttctgttctc actatcttag atatattcat
    12781 ccaacttgtt atccctatta ctatggacat agatactgga tttgaatttc caagtatagc
    12841 ttgtacaaat attataatta atattgaagg tatactaagt atgatttctg taaacctcat
    12901 cattgcatca tctacaagtt cagatgctga accacttata ctaccataaa caattcctat
    12961 aacagttgat attacagtgg aaaatagtcc tataaataaa gatatttttc ctccatacca
    13021 tatcatagaa tatatatctc tacccattga gtctgttcca aataaaaaag ttttatttgg
    13081 agctaaatta gaacttgcta aatccatgta tgttggttca tgtgtcatta taaaagatga
    13141 gaatacactt ccaatgacta tgattgataa aataataatt gatatatatg gtaattcttt
    13201 aattttttta gataaggttt tctttgtttc tatatgttca tcttctgcta ctaaaatata
    13261 attttctcct acaatttcaa aatcactata ttgtggcttc attagtatca cctctatcat
    13321 atttcattct agggtctatt ttattgttta taatttgagc taacatattc ccaaatacta
    13381 ctaaagcccc tgttattaag cataaaacta ataacatatt ataatcatga tactttgcac
    13441 tttcaaaaca aagtgttcct aacccaggat aagaaaaaac tttttcaact acataagttc
    13501 ctcctagtat atgaggtatc gaaatggcca ttatactaat atatgatggc attatatttc
    13561 tcaggcaatg cttaaaaaca atagttctat tatttaaacc ttttgcttta cataaaagca
    13621 cataatcttc tcttatttcc tctagtaatt tatttcttat catatatgtg taataccata
    13681 gatggcttaa tataagtact gtaagaggta agattagatg tgatatccta cttaatatac
    13741 tggactcttc tcccattgaa taagcaccac tacttggaag tatacttaag tttatactaa
    13801 atataagtat gagtactaag gcaacccaaa atgatggaat acagttggtt attgttccta
    13861 atttgcatat gattctatca atgagcttat cttcatgtaa tgtacaaaat attcctaaca
    13921 ataaggctag cacaaaggta agtatatatc ctgaaccacc taatattata gtgtttatat
    13981 agacatcatt tataacagaa gtaacatttt gtttatattt aaatgaaatt ccaaattccc
    14041 cttttgatgc atttgaaatc cattttatat attgactgta tattggttca tccaaaccaa
    14101 gttttttcat agcattttct ttttcttgtg tactcattct ttctactcca tccccatagt
    14161 aagagattaa tgggtctcct ggagcaagtc ttgccatgta aaaaacaata aaagataata
    14221 aaaacattac taaaataaac tgcaacaact ttttcagtat atcctttaac aaaccccaac
    14281 ccccctttgt tgcaataaaa aaaaccacat aaaagcaaac gtaaagtaac gttaaaacct
    14341 tcgtggttcc attatcatta aacaaaatta aattttctat atttatctat gagttaattt
    14401 tagcatcatt tattaactgt gtaaacaaac tttttaaaaa ttgttaaaat ttcatcaaat
    14461 tattttattt atacctaaaa taaaattttt atattctatt cttcattatt ttctataaaa
    14521 ttataaaatt ttaggttgac tatataagaa aagtatatta tattagtttg tatgcaaacc
    14581 atttaagagg tgattttatt gaaaagtaat tatgactgtt ttagaatagc catgctctta
    14641 aaagaacttt attctaaaac tatgtacaca gtagaagaaa attttaaaga aaatggatta
    14701 acacaccaac agattatagt tataaaactg atagctcata atcaagaact tacaatatca
    14761 caactatgtg atgaaatgtc tttagctaaa ggtacggttt caggcataat cagcagatta
    14821 gaacagattg gatatataga aaaattcaag aaatctaatg ataaaagaaa tacatacgtt
    14881 aagtttacaa caacaggatt tgaatttgcc acaaatttta aaattaaaat gcaagaaagt
    14941 tttgatgata tttttaaaaa ttgtgatgaa aacgaattga gtgacttagt aaaaaatctt
    15001 agaaatatat tggcaaaagt aaaggagaga taattatgaa ttataaatta atacttgcaa
    15061 tagtatttat aacatcagct ttaattttct acactatagg tgtatttgga gaaagaaagg
    15121 caaaaatttt aaaaaagaaa catgtaatta tattttggct tggttttata tttgatactt
    15181 taggaacatt tacaatgagt aacatagcca acagccatac ttttgaagta aaatctgctt
    15241 tatcacaaaa ccttcatagt attacaggtc ttcttgcaat tgtgcttatg ctttttcatg
    15301 caagttgggc tacttttgtc ctatacaaag atgatgagga aaagaaaaaa ttctttcata
    15361 agtttagtat agttgtatgg actatatggt tagttccata ttttataggt atgtttattg
    15421 gtatggctgg atagtaaaat aaaaagtgtg taagaaatac tgtaggtaaa tttgtataag
    15481 acaaatttat ctacatcctt ctttcacact tttacatatt tatttataat actccattat
    15541 ttctttcatg tttcttaata cctatataga atatttaatt gtttctatag tattttat
//
LOCUS       DAMJYX010000081        15274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJYX010000081 DAMJYX010000000
VERSION     DAMJYX010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.082"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            243..932
                     /locus_tag="PC479_002670"
     CDS             243..932
                     /locus_tag="PC479_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805205.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            1368..1907
                     /locus_tag="PC479_002671"
     CDS             1368..1907
                     /locus_tag="PC479_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805206.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            1926..2969
                     /locus_tag="PC479_002672"
     CDS             1926..2969
                     /locus_tag="PC479_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805207.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            2971..3807
                     /locus_tag="PC479_002673"
     CDS             2971..3807
                     /locus_tag="PC479_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805208.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            3912..4586
                     /locus_tag="PC479_002674"
     CDS             3912..4586
                     /locus_tag="PC479_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805209.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            4586..5638
                     /locus_tag="PC479_002675"
     CDS             4586..5638
                     /locus_tag="PC479_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2805210.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            5868..6716
                     /locus_tag="PC479_002676"
     CDS             5868..6716
                     /locus_tag="PC479_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2805211.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            6706..8370
                     /locus_tag="PC479_002677"
     CDS             6706..8370
                     /locus_tag="PC479_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2805212.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            8419..9675
                     /locus_tag="PC479_002678"
     CDS             8419..9675
                     /locus_tag="PC479_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2805213.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            9698..11779
                     /locus_tag="PC479_002679"
     CDS             9698..11779
                     /locus_tag="PC479_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2805214.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            11816..12901
                     /locus_tag="PC479_002680"
     CDS             11816..12901
                     /locus_tag="PC479_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2805215.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            12955..14079
                     /gene="wecB"
                     /locus_tag="PC479_002681"
     CDS             12955..14079
                     /gene="wecB"
                     /locus_tag="PC479_002681"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2805216.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            14157..15131
                     /locus_tag="PC479_002682"
     CDS             14157..15131
                     /locus_tag="PC479_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2805217.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         6198 a         1451 c         2606 g         5019 t
ORIGIN      
        1 aatataaatg aaattgaaaa catttgaagg atagaaattg gttggataaa tgtataaatt
       61 acttcaccaa aaaataatga caaaaattgt aactaagttg taataaaatg gtagttattt
      121 agcataaaat aatgtatcat agaagtatat tataaaatta ataaaataag atttttgtaa
      181 gaaaactgct ggcttttgat atttagagtg agtagttaaa ttatgtttag gaggggtaga
      241 atttgttgca aaaagtatta tggaaatttt taaagctagt agtcggttta tttatatgtt
      301 cagttggtat agtattgacg ataaattgta accttggatt atctccctgg gatgtatttc
      361 atcaaggact ttcaaatcat ataggaataa ctataggaac tgcttctata attgtaggct
      421 ctatagtagt tattgctgat gtcgttcttg gagaaaatgt aggatgggga actgttttta
      481 acatgctact aattggattc tttatggatt tacttttata tagtaattta attccagagg
      541 ctgatagttt atttgtagga ataattatgt tgatattagg attggttctt ttgtctgttg
      601 gaatggtatt ttatatggga agtggtttag gaagtggacc tagagatggt ttgatggttg
      661 caattcaaaa gaaaacagga aaatcactta aattaataag aggtactata gaagttggag
      721 cattaatagt aggattttta ttaggtggaa aagttggtat aggaactata ataagtgcat
      781 ttggattggg gtatttcaca caaatggtat ttggcttatt taaattagat tgtagtaaga
      841 ttaaacatag atttatagta gatgatataa aatttatcaa agtgtatatg agtggtgaga
      901 aaaaagctgc tgttcaaaat atagttaagt aatttaagaa taaattcaac gaaatgttaa
      961 gtattttaaa ttaacatttt gtttagatag ataaattgaa tattaaaaga atattaaaat
     1021 atgaggattg ctagaacact ataaaatata gatttctagc aattttttat ttgttcaata
     1081 tttttttagt actagaagtc ttattctcta tttaatgaat gaattaattt ataattagag
     1141 atactaaact ttatagatat taatataaac acctttaaac tgtgtatttt ccaaagcctt
     1201 taaaatgctt gtatttattc taaataaaaa aataaaaaaa tgtcaaaaaa actcttgtat
     1261 tatatccaaa ttttatatat aattacctat gttgtttaga aattttaaac tcaatgttta
     1321 gtaaacaaaa tgtttagtaa aataaaacag aaactaggag gtgcaacatg gatataggtg
     1381 aaaagataaa aagattaaga acagaaaaac aactgaccca ggaagagtta gctaatagat
     1441 gtgaattgtc aaagggattt atttctcaat tagaaaataa cttaacatca ccatccatag
     1501 ctacccttat agatatactt gaaatactag gtacaaattt aagggaattt tttaatgaaa
     1561 tagatgatga aagaatcagt tttacaaaag aagatatgtt tgaaacagaa gatgaagatt
     1621 taaaatataa attaaagtgg ttaattccaa attcacaaaa aaatgaaatg gagccaataa
     1681 taataacatt gtatccagga ggacagtata aagaagaaaa gccacatgaa ggtgaagaat
     1741 ttggatatgt attagcaggt tcaatatatg tccatatagg cgaaaagaaa aataaagtaa
     1801 aaaaaggaga gagtttttac tttagaccga aagcaaatca ctatatatca aacgagggca
     1861 aaactacagc aaaagtgatt tgggtaagta cgccaccatc attttagaat agaggtgttt
     1921 aataattggt agaaaatata atagaattaa gggatatatc taaacattat gaagaactta
     1981 ctgttttaga taatttcaat ttagatataa agaaaaatga gtttctaaca ttacttggac
     2041 caagtggatg tggaaaaact acaacattaa aaataatagc aggatttgag tatgctgatg
     2101 atggtaaagt tttatttgaa ggtaaggaga taaataattt gccaccatat gaaagacaag
     2161 taaacacagt atttcaaaaa tatgctttat ttccacacat ggatgtttat gaaaatgtag
     2221 cttttggatt aaaaattaaa aagactccaa aagatgtaat agatgctaaa gtaaaagagg
     2281 ttttgaaact tgttgcacta gaaggttttg aaagacgaca agttgaatca ttgagtggag
     2341 gacaacaaca aagaattgca atagcaagag ctctagttaa tgaaccaaag gtattattac
     2401 tagatgaacc acttggagca cttgatttaa agttaagaca agagatgcaa atagaattaa
     2461 agagaatcca aaaaaaatta ggaataacat ttgtttttgt aactcatgac caagaagagg
     2521 ctcttactat gtcagatact attgttgtaa tgtataaggg caaaattcaa caaatgggaa
     2581 caccacaaga tatatacaat gaacctaaaa attcatttgt agctaagttt ataggtgaaa
     2641 gtaacatatt tgatggaatt atgatagaag attataaagt taatttctgt aatagagatt
     2701 ttgagtgtgt tgataaagga ttcgaaaaaa acgaaaatat agaagtagta ataagaccag
     2761 aagatataaa aatggtaaag ccagaagaag gaatgttaaa ggggaaagtt acatctacag
     2821 tgtttaaagg agtgcattac gagatagaat tgaatgaaaa tggaagattg tggatacttc
     2881 ataatactaa aaatgctgag gttgggactg aactaggcat ggatatatat ccagaagata
     2941 tccatataat gagaaaagag aataactaag atgaaaagaa aatctttttt agcatatcca
     3001 tatgtagttt ggagtgcgat atttgtaata attcctttaa tattagtagt attttttagt
     3061 tttacaaaag aatcaggtgg aggttatgcc tttacacttg agaattataa agaagtaatt
     3121 gaccctatat atatgaaagt ttttgggcgt tctattcttt tagcaggagg agctacatta
     3181 atatgcctta tagttggtta tccagtggca tatataattt caaaggctcg tgtaagtaga
     3241 agaggttcat taatattatt gtttattctt cctatgtgga tgaactttct tttaagaaca
     3301 tatgcatggg ttgctatatt aggaaaaaat ggacttttaa atacattttt aggttggttt
     3361 ggaatacaac cattagcaat tttgtatact aattttgcca tacttttagg tatggtgtat
     3421 aactttttac catttatggt tcttccaata tatacagcac tatcaaagat ggataatgat
     3481 ttgataaacg ctgctcatga cttgggtgca aataatatga cagtttttag aaaaattata
     3541 tttcctttga gtttgccagg tgtaatgtct ggaataacta tggtatttat gccagcagtc
     3601 actacttttg caatatcaag actgttaggt ggaggtaaaa taatgctggt tggagatttg
     3661 attgaacaac agtttacagt tgttggagat tggaactttg gttcagctat atcgatattt
     3721 atgatgatag taatattaat atctatgtct ataatgtcaa aatttggaga tgaatctgac
     3781 aaagaaggcg gtgggttact attttaaaat taaagaaaat aacttctaat atatatttag
     3841 cattagtatt cttatttttg tatgcaccca tatttgcttt ggtactattt tctttcaatg
     3901 attcaaaatc aatggcaaga tggaatggat ttacatggaa gtggtatggt caattacttc
     3961 aaaatgagag tataatgagt gctttatatt atactattgt aatagctata ttagcgtctg
     4021 taatatctac aatagtagga actataagtg ctataggaat acataagatg agaggtaaga
     4081 gtaagaaatt aatactaaat gtaaactatc ttcctatttt aaatacagag atagttacag
     4141 cagtagctct tatgagtctg tttgtatttg taaaaatgga atttggattt acaacaatgc
     4201 tattggcaca tataatgttt tgtttgccat acgttatact ttcagttttg ccaaaaatga
     4261 agcaattacc tgataatata gaagatgcag ctatggactt gggagcaaca cctatatacg
     4321 ctcttagaaa agtaatttta ccacaaataa aaccaggtat agtttctgga tttttaattg
     4381 catttactat gtcaatagat gactttataa taagtttctt taatgctgga aatggtgtaa
     4441 gcaatctttc tatagaaatc tatggaatgg ctagaagagg tataaaacct gaaataaatg
     4501 cattatcaac aataatgttt gcagtggttc taggtttact tttactagct aataaaaaag
     4561 aatctatagt aagggggatt aaataatgat taagtttaaa aaagcaatat cccttatagc
     4621 tgtaggtatg atgacagtta ctttatttac aggatgtaat aaagcaactg attctacaaa
     4681 agttttaaat gtatataatg ttggagatta tatagatgag tctcttatag ataagtttga
     4741 aaaagaaaca ggaatagatg ttcaatattc aacttatgat actaatgaaa tgatgtatca
     4801 aaaagtaaag agtggtagta cacactatga tttagtattt ccttcagact atatggtaga
     4861 gaaaatgaaa aatgaaggtc ttttggaaaa attagatttt aagaatatac ctaatatgaa
     4921 atatttagat aagagttttt taaatcctgt atacgacaaa actaatgaat atagtgttgc
     4981 atatatgtgg ggaactcttg gaatactgta caataagaaa gaagttaaag accctatgga
     5041 tagctggaat atactttgga atccaaagta taagggaaat ataatgatgt ttgactctgt
     5101 tagagatact atagggataa ctcttaaaaa attaggatat agcatgaata gtgtaaatcc
     5161 aaaagaaata aatgaagcta aaaatctttt aatgaaacaa aaggatttag tattggctta
     5221 tgtaaatgat gaaggtaagg atagattgct tggtggagaa gtggcaatgg gtatgctgta
     5281 ttctggagat gcagttacac taatggagca aaatcctgac cttgattatg ctatacctaa
     5341 agagggaact aataaatggg tagatgctat gtgtgtacct acaacagcac aaaataagaa
     5401 agaagctgag ctgtttataa actttttatt agacccagag aatgcaaaag taaatgctga
     5461 gtacattggg tattcaacac ctaatacagg agcactaaaa ttattagacc cagaaataac
     5521 tcagaatcct gttgcatatc catctaaaga agtgcttgat aaatgtgaaa cttttgaaga
     5581 tattggtgaa aatataaaat tatatgacaa ggcttggata gagttaaaat caaaataggg
     5641 tcttgttggt ttaagtaaat ttacttaata aatacttaat aaaactgttc ttttttagaa
     5701 aatttatttt ctaattattg aacagtttta ttttttgttt aagttatatt catatgcaat
     5761 ttaaatagtt tattaataaa tgaaaactta tttgtataat gactttaata ataattagaa
     5821 aaaatgggca acttaataga ataattggag gtgtaaattt ataaaaaatg aataggataa
     5881 ataaaaaaat agatttatta atattggctt tgctcgtttt tatattttta attgttggaa
     5941 tagtaatact atcgattagg acagaaaata aattgaatgt gttcataatg ttagcaataa
     6001 ttttttttat aataatgtta acttatttat caaattcagt agtggggttg ataacaagtt
     6061 caataattat atttatgtat acatcctata ttttatataa caatattact cacaatatgg
     6121 atgttgaatt tatatcatat atgtggataa tagcaacacc agtatcttca attataatgg
     6181 gtaatctgaa caaaagcata aatgaattgc aaaatacaaa taaaaaacta tctgaacagt
     6241 ataaggaatt agtgactatt gatagtgaaa ctggacttag aaatttaaag attttttata
     6301 atgatgtaaa tatggagata agtaaatcta ttagacataa tactgatttt tctcttatga
     6361 tagtaaaatt accatactac ggaaacctac agactatatt tggtgaaaac aaaactaata
     6421 aaatagtaaa atatattggt tcaaatatta tagaatgtac aagaaatgaa gatataattt
     6481 atagtttaca aaaagatatg atagggatac taatgccaaa tacatctttg gaaggttcaa
     6541 aagttgttaa agacagaata aagaaaagga taaaagaatt aaatttagat ttaaacaata
     6601 ggggaaaata tgtaaatata gatgtcaaaa ttgcattttt acaatataag agtagttttg
     6661 gagatagtat aaattttaag aatatagttg aagaggaact tcaatatgat gtttaaaata
     6721 tttaataaaa gtctattaaa attagtagtt gtattatcaa tagtactgac aatatcaaat
     6781 tttagcttat cttcatatgc atcagaaatt acttccagca cagataaggt attaatcata
     6841 tatgattcta agaaagaaac agcttataat agagatattt taaatattat gaggactcta
     6901 ttgggaagat ttagttcaga tatagagcta ttaaaattga gtaactatga tggtgagata
     6961 aataaaaatt attacagtca tatatttatt ttaggaatca atgaaaactc atataataat
     7021 gataaaaaca ctaaaaactt gattagttcc ttaaatttat ataaaggaac tatatgttgg
     7081 ttaggatatg gtatagaaaa tcttttggaa cataaaaaat acaatttaga ctatgtagga
     7141 aaaacaaaca atatagttag tgtaaattat agaggtaaat catataactt ggatgaacat
     7201 tatgttttta acattgtaga atcaaaagat acaagtaata aagtaatagg aagtattaat
     7261 gataccttaa ataagtatcc atatataata aatgataaaa atctttttta tgtatcaaaa
     7321 ttagatttag atggagtttt attttatata ttttgcgatt cacttaatga tatttttaat
     7381 attaagacgt ttgataaagg acgtatattt gtaagaatag aagatgtaca tgcttttaga
     7441 gaaccaaaaa atcttgtaga gatagcggat tatttaagta gtaagaatat accatttacg
     7501 attgcattaa taccagcata tgtaaatcct aaaaatcata aggtaataac tttatcagaa
     7561 agccctgaaa ttgtcaaagc aataaaatat atgcaagata aaggtggaac tgtgatattg
     7621 catggatata ctcatcaata caagaaagaa gaagtttctg gagaagggta tgaattttgg
     7681 gatggtaaaa aagatgaacc cttaaaagaa aatatgaaga tatttgtcaa agatagagtt
     7741 ttgaatggtc ttagagtatg tatagagaat ggaatttatc ctcttgcatt tgaagcgcca
     7801 cattatgcaa tggaatcaga gggatataaa gaattaaaaa aatatttttc tacttatata
     7861 gggcaacatc aaaataatga taaaaaattt agcacaaata catatcctta tattattagg
     7921 gatacagagg agtttaatat atttataccc gaaaatttag gatatataga cccagaagat
     7981 aagtttactt ttcaaaatat aaaagaaaat ttagataaat tatccattgt aagaggtttt
     8041 agtggagggt tcttctttca ttcatattta aacatagagt atctaaaaaa cacaatagaa
     8101 tatttagaaa aacagaatat agaatttatg aatttaagag attttaataa ttgggtaaaa
     8161 gttgatgaaa tacaaataag aaataatgga gatgaaatta tagtcaacta tgataaggac
     8221 ttagatgaac taactaaaag tgatactaga tttaaatcca tatccaatat tagtaaagtt
     8281 ttaatattca tagtatctat aagtgtttta atatttgtga ttatatttat atattttaag
     8341 agaatagata aaaagaaatt tttaaaataa aggattttat attagaatca atattttaaa
     8401 atattttagg agaaatttat gagagattta aatgtatcag agtatttgtt cgtatttagt
     8461 ttgtttagta tatggtcttt attgcttata aatattattt tagctatggg tggatatata
     8521 ttttatttta aaaactttga taaagaaata aaagaaattg atgaatatcc tatgatatca
     8581 atacttgttc ctgctcataa tgaagctaaa gtgataggta gaactgtgga gtctttgcta
     8641 ttactcaatt atcctaagag taagatggaa cttattgtta taaatgacaa ttcttctgat
     8701 aatagtaaag aaattttaga aaatataaag gataggtaca acaattataa tttcactata
     8761 ataaatacag atagtttaac aggtggaaaa ggaaagtcta atgcattaaa tattggatat
     8821 acgatttcaa aaggagattt tatagctgtt tatgatgcag ataacacacc agataaaaat
     8881 gcacttagat atctagtaca aactatagta atgaatgatg aattaggtgc tgttattggg
     8941 aaatttagga ctcgaaataa aaataaaaac ctattaacca aatttataaa tatagaaaca
     9001 ttaagctttc aatggatgtc acaagctggt agatggcaac tttttaatct atgtacaata
     9061 ccaggaacta actttatatt gaggagaagt atcatagaag aaattggagg atgggatagt
     9121 aaagcaattg cagaagatac agaaataagt tttaggatat ataaattggg atacaagata
     9181 aagcttgtac cacaatctat aacatgggaa caagagccag aaactgttaa ggtgtggata
     9241 aagcaacgta ctagatgggc aaaagggaat atatatgtat taatgaaata tataaaaaat
     9301 atatttaaac agggaagaaa taagattgtg tttgatatag cttacttttt ttctgtatat
     9361 tttctatttt taacatctgt aattatatcc gatattttat ttgttttaag tatatctaaa
     9421 ctagtagaaa tttctattcc aattaacttt tttctaatat ggatactatc ttatctttta
     9481 tttattatag aagtgagtat aagccttact atagaaaaag gtgaagcaac aattgaaaat
     9541 atattcatag tggcaataat gtattttaca tactctcaac tatggctgtt tgtagctata
     9601 aaaggaatga tagagtatct aaaagatata atttttaaaa gagaggttaa atggtataaa
     9661 acagaaagat tttgattcat gtacaggagg aattaacatg aaaaaattta ttatatcaat
     9721 tatatcttta gtattgtttt tttcaaatat atcacttata tacaaagtaa atgctgatga
     9781 gactaaagtt aaaaattata agtttgaaag agatataact attgatggtg ttataggaag
     9841 taatagtact ttttttgaag taaataaaaa ttgggatatt gaagaagttt tattgcatct
     9901 taattttagt aagagccaaa tattaaatgg agatgtttca agtttaactg tattgataaa
     9961 taatgtacca ataaaatcta ttaaattaaa tgctaagaca aattataaaa atacattaga
    10021 agtattagtt ccaaaagatt atataattca aggatataat gaaataaaaa taaaaacata
    10081 caaaacaata tctgataaaa tttgccaaga tgattccaat actgggaact ggatggttat
    10141 tcacaaagaa tcatatatat caattagata taagcagaaa aaggtggaaa actcaataaa
    10201 tgaatatccg tatccatatg cagaaattga aaataatcat aaattagata caactatagt
    10261 agtaccagat aatatgacta gaggagagac tacagcagtg tttaatttgg catcagcatt
    10321 tggaaaaata acaaaaaatg atgatttaaa gttagatgtt aaattatatt cagaaatgaa
    10381 aaattggtca gatgataata ttatatatat tggtaaacct gaaaatacag cagaagaaat
    10441 tttggatatt ttaagtataa aggaacaaac cctattatct tcaaactgta taatcaagca
    10501 agtagattca ccatacaata agaataagaa gatgatggtg gtaattggta gtaatgaaga
    10561 tgatttaata aaggcatcaa atttattaat agaaaataga ctttctaacc aagttttatc
    10621 atcatcagta cttgtaaaca aagagactaa tataaaaatt aatagggaac aaaaattaaa
    10681 tttaggtcat ttaacattaa aagatttagg atattctgac tttttgttgg aaggagcatt
    10741 taatcaacaa gctttgtttg atgtcaaaat accaacgggt aaggtattag atgatggttc
    10801 aaagattatc ttaaatttaa gatattctga caatttagat tttgaaaaat ctttagttac
    10861 agtgtctata aatgatgtta ttgtgggaag taaaaaattg gataggtcac attctaataa
    10921 tgataagtta gaattaaaaa tacctaaaga tatagataat aaaaactatt atcaagttaa
    10981 attgacattt aatttgagta taaaaaattc gaattgtgtt actagagaga gtaataatcc
    11041 ttgggcatat gtatcaaata attcttattt ggcgttatct acgaaagaaa atgaaacctt
    11101 atcttttgaa aattatccgt atccatttgt tagagatgat gaatttaatg atttaactgt
    11161 gataatgcca gattattcag ggtcacaagc tatgacctgg atgtttagat taggtgttac
    11221 tttaggtgcg aatataaatt cacataatgg aaatataaat gtaattagag gaaaagaatt
    11281 tagtgacaag tataaggata ctaatattgt agtttttgga gtgccacata ataattctgt
    11341 tataaagatg ttaaataata atttaaatat aaagttcgat aaaaattact ccaattttat
    11401 atcaaatgat aagataagct ttattgatga ttatggaaaa aatatttcta ctattcaatt
    11461 aataaaatca ccatataata atcaaaaaaa tatcatggta attagttcaa tgaatgaaaa
    11521 aaacttatat ttaggaatgg attatttgtt gaataaaagt aaagttaatg atttaaaagg
    11581 agatacattg ataattgatg aatacggaga ggtagaagat ttagcttata atttaaaatc
    11641 taagaaagaa gtaaaagatt caagttggaa tatgagtata aataagacaa ctaaagtatt
    11701 tttaatgatt tcttttatta ctataattat agtcatgata ttatcaatgc tatatataaa
    11761 aaaatataaa agaagataat atgataaatt tattatttta aaagaggtat ataatttgag
    11821 gaaatatatg aatgaattta aattaaaaag aagtatttta atctcattaa taatcttaat
    11881 ttttatattt atagtttact ttgtaagtat tgagttaaaa gataggagtc tagtaaataa
    11941 tcaatataaa ggtaagatta aatcggccaa tttatctgta gattatgaaa taaatcaagt
    12001 tatgaaggat atagataaat tgggattgaa tacagtaaat gtacctataa ttataaatgt
    12061 agagtccatt aattctgata ttatgtcaat agataataat agtaaagaaa aggcaattaa
    12121 gttaataaaa aaattaaata aaaaaggtat ttcaacaata ttagaggcat atccatggat
    12181 tgaaaatgga aaattgtatg agactgactg gaaccctata aataaaaaaa gattttttta
    12241 tacttggcaa aatgccatat taaatgaact tataattgat gtagcaaatc cactggatgt
    12301 aaatgtttta aatattggtt ctaattttgt acatttggaa gaatatcaac aaaattgggg
    12361 tgaaataata gattttgtac aatccaaatt tgacggtctt gtaacgtaca gaactaattg
    12421 gtggtataca aaaaaagatg atttaagtag taagttgttt tatgaacaaa agctgaacaa
    12481 taattttttt gataagttag attttatatc tatagcagca tattttgagt taagtaataa
    12541 acctgttaat acagtggatg aattaatttc tgccttacac tcatctactg taaacaatag
    12601 aggacaaaat ataaaacaag aaatatataa cctttataaa gctcatagaa aacctatatt
    12661 ttttggagaa ttaggttttt caaatagaga aagtgcaagt tctcagccgt ggaatcatac
    12721 tccttcaaaa gctgtaaatg gcgaagaaca agcaaggtgt tttgaggcat ataagaaagt
    12781 ttttgaaaat gaagactgga taaatggttt ttctgtgttt tgtgtaggta aaatagatga
    12841 cgaaaaaaat ttttacccat caaaagagag tataaaagtt attaagtctt ggtatgaata
    12901 attttgaaag tgtagaattt atatagatta aatagatgat taggagagat ataaatgaat
    12961 gacatcaagg ttatgactgt atttggaact agacctgaag ctatcaaagt agcaccttta
    13021 ataaaggaac ttgaaaaaag agagaatata aaaagtattg tttgcgttac agctcaacat
    13081 agagaaatgc ttgaccaagt tatagaaacc tttaatataa atgtagacta tgatttggat
    13141 ataatggaaa aaggacaaag cctgaatgat ataacatgta agattttaaa taaacttccc
    13201 ttgattttaa acaaggaaaa tccaaatata atattggtac atggagatac aaccacaact
    13261 ctagctacaa gtttgactgc attttataat aaaactcttg ttggtcatat agaagctggg
    13321 cttagaacct atgataaata ttctcctttt ccagaggagc taaatagaca acttacaggt
    13381 attattgctg atatgcactt tgctcctact aatttggcta gaaaaaactt gatttctgaa
    13441 ggaaaaccta ataataatat atttgtaact ggtaatactg ctattgatgc tttaaagatg
    13501 acaataaaag aaaactataa tcatcctata attgatgaga ttggtaacga taggatgatt
    13561 ttgttaacct ctcacagaag agaaaatttg ggtaaaccta tgaaaaatat attcagagct
    13621 ataaagcgaa tagtagatga ctttgaagat gtacagatag tatatcccat acatttaaat
    13681 cctaaaatac gaactatagc tgatgagata tttggaaaat ttcctgaaaa aattcatatt
    13741 atagagcctt tagatgtagc tgattttcat aactttttaa ataaatctta tatgataatg
    13801 actgatagtg gaggaataca ggaagaagct ccatcattag gtaagccagt tcttgtgttg
    13861 cgtgataaaa cagaaagaac agaaggaatt gaagcaaaga cacttaaatt agtaggtact
    13921 aatgaagata gaatatataa ttcagtgagc gatttattaa taaataaaga taactatgtt
    13981 cagatgagta aagcatctaa tccatatgga gatggaaatg caagcaaata tatagtggat
    14041 ataataatta aaaaatttaa ttgcaaatat ttaaattaga taaggcctaa aacattaact
    14101 aattgatata agatttatga taatattaag gaatacaatt tttaaggggg agtaatatgg
    14161 ctagaaaatt aataaaaaat ttgggtaaaa gtaaaagtgt aaagagagtg aaacttttat
    14221 ttaagaagat ttttattact gtattcatag tagcaagtat agttgctatt tttaatataa
    14281 cgaaatattt tgaggaatta tacaaagtaa gggatttaaa aagtactaaa attgaatact
    14341 atatggatgt ggctgatgaa gcaggagatg gaaaggtcca attaagttgg aaggccttac
    14401 ttgctataga catggtaata catgatgaag atttaagtaa tataaaaaag aaagacacat
    14461 tggatatagg ggaaaagttt atagtagaag ataaaaatgg taaaggcgaa aaggtgtata
    14521 aggtaaaaaa gtttaataag gtattaagcg aattgaaatt tgactcttct caaaaaagta
    14581 gagcaagaaa atacatgaaa gatttagaat acacatacct tggaaataaa caattagata
    14641 gtagtgatga aaaaattaaa tttataaaga agttagaaga ctcagctata agagaatata
    14701 ttgattatgg aatattgccc tctataacaa ttggacaagc tatattagaa tctggttggg
    14761 gaaattctaa acttacaaaa cagagtaata atttatttgg tataaaagca gataaagcat
    14821 ggaaaggaaa aagtgtagaa atttcaactt cagagcatta taatgaaaaa attgtagcta
    14881 gttttaggtc gtataattca ttacaagatt ctgtcaaaga ccacagttta tttttaatta
    14941 ataataaaag atatagaaaa catgggttgt ttgaggcaaa agattatatt agtcaagctc
    15001 aagcattaga aaatgcagga tatagtacag cagaagataa aaaaggaaat cgtatatatg
    15061 cagagctatt aatagatgtt attaggagtt ataatttgca attgatagat aataaagttg
    15121 agacaaaata attgttaact ttatatgagg agataacaag ataagatttt tttctacaaa
    15181 aagaatagaa aaattgtaaa aaaaactaaa aaaatgtatt gacttgttat ttaaaaggtg
    15241 atattattat tactgtactc gaaagagaac aaaa
//
LOCUS       DAMJYX010000082        14584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJYX010000082 DAMJYX010000000
VERSION     DAMJYX010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.084"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            106..1176
                     /locus_tag="PC479_002683"
     CDS             106..1176
                     /locus_tag="PC479_002683"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805218.1"
                     /translation="MNKNQIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCKHCCVESSS
                     NKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILS
                     TNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFS
                     NIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPIT
                     IPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFD
                     NEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELEL
                     DRWCSMMKHNLNTAVWGDEKVN"
     gene            1173..2189
                     /locus_tag="PC479_002684"
     CDS             1173..2189
                     /locus_tag="PC479_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805219.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            2245..2406
                     /locus_tag="PC479_002685"
     CDS             2245..2406
                     /locus_tag="PC479_002685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805220.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            2614..5160
                     /locus_tag="PC479_002686"
     CDS             2614..5160
                     /locus_tag="PC479_002686"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805221.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            5202..6446
                     /locus_tag="PC479_002687"
     CDS             5202..6446
                     /locus_tag="PC479_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805222.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            6494..7483
                     /locus_tag="PC479_002688"
     CDS             6494..7483
                     /locus_tag="PC479_002688"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2805223.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            7520..8194
                     /locus_tag="PC479_002689"
     CDS             7520..8194
                     /locus_tag="PC479_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805224.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            8178..9920
                     /locus_tag="PC479_002690"
     CDS             8178..9920
                     /locus_tag="PC479_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805225.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(10335..11579)
                     /locus_tag="PC479_002691"
     CDS             complement(10335..11579)
                     /locus_tag="PC479_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2805226.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(11622..14456)
                     /locus_tag="PC479_002692"
     CDS             complement(11622..14456)
                     /locus_tag="PC479_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805227.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
BASE COUNT         5747 a         1526 c         2212 g         5099 t
ORIGIN      
        1 aagtattttc ttttaggaca agaaaataaa ataaaaattt atgattttgt aaattatttt
       61 gatgatgaaa gtgacaaaaa atatattatt aatcttatag atattatgaa caaaaatcaa
      121 atttttaaaa gtaacaattt aaactttgat atgttaagca atggtgttgc aatttatttt
      181 tgtttgacaa atagatgcaa tttattttgt aaacattgtt gtgttgaatc ttcttctaat
      241 aagaaagatt tacttagtac atatgattta aaaaatatcg tagatataat atcagtttta
      301 aatactcctc atataattat aactggtgga gaaccattga taagagaaga tttttttgaa
      361 ataattaaat atataaaaca aaaattacct gataccaaat taatattatc cacaaatgga
      421 actttaatag atgaacataa tgttgatttt attataacta attttgaaaa aatagatatt
      481 agtattgatg gagtagatga aaatacttgt agtgaaacac gaggaaaagg tgtatttaat
      541 aaagtaataa gctctgttaa tctgctaaaa gacaatggat tcagtaatat aaagctgtct
      601 atggtatttg gacaaaaaaa ttctcattta aataaacaat ttaaagattt aaataaaaag
      661 cttggaacag aatttgttga aagatatttt attccaacag gaagaggcct agaaaataaa
      721 gaaagctatt taattgaaga tacagttctt ccaattacaa ttccgtatat tttagataat
      781 gaattaaaaa tgtgtaaaga taaaaaaaat aaaaaaatgg gatgttgtgc atgtaatgca
      841 ggaaactctc aaatatttat aaattatgat ggagatgtat atccttgtcc ttcactgata
      901 aaggatgact ataaaatggg aaatatattt gacaatgaat ttgtaaaagt tataaaagaa
      961 aataaaacaa gagatctaaa gtctttgatt aaattagaaa gtatttatcc atataacttt
     1021 gaaaaatgta aagattgtga tgtaaatatt ttttgttggc attgcccagc gatattagat
     1081 tcagttaagg ataatgaatt ggaattggat agatggtgta gtatgatgaa acacaattta
     1141 aatacagctg tatggggaga tgaaaaagtt aattgaaatt tactatattt gtaactgaag
     1201 actgcaattt gagatgtaca tattgttatg aaggtaatga taaaaaaaaa gaaaatatga
     1261 gttatgaaac tgctgataaa acaatagatt ttatttgtaa taaagaatta gaaagtaata
     1321 gtgattatac aacaattgtt ttacatggag gagaaccatt tataaatttt gatttgatta
     1381 aatatataca tagggattta gtagatagat tgagtgacaa aaagatatta tttgatataa
     1441 ctacaaatgg aactattata aatgatgaaa taatagagtt tatagcaaag aatgtaaaaa
     1501 acatatcgat aagtattgat ggaaaaaaga atatacatga tataaataga atagatgcta
     1561 acggagtagg tacttatgaa aaagtaatgg aaaatggaaa aagacttata gataaaggta
     1621 tatatgttag ggcaagaatg acatataact caaatactgt taagtatctt tatgagagtg
     1681 tatatgatat agcaaaacaa gggtttaaaa atatcgcacc aatttcagat tttatggata
     1741 aaaattggga taattctcat attaaaatat taaatgaaca aattagtaaa attatgaata
     1801 ttaaaaagga ttatcctgat atagatatat ctattttaga taaacaattt ataaataaga
     1861 aaaaaggaga ttgttttgga gggataaata gttatgcaat agatactaga ggagatgtat
     1921 atccatgtac agtatgtgta ggagataaag atattttact aggaaatata tatgatggat
     1981 tagatgataa aaaaattaag aaccttataa aaatttatat atctaagaat gaagagtgtg
     2041 atggatgtac tagacttgat tattgtatgt caaccagatg taaacttatc aataaaaagg
     2101 taacaggtaa attccaaagt gcagtaccaa tttattgtgg attagaaaga gtttatgtgg
     2161 aaacaacaaa aaaactatgt agtttataat ctagtttaac tagttataat aaatataaaa
     2221 caaaagtaaa gggggatatg tattatgata catataaaaa aaatcgaaga aaaaatagaa
     2281 cttcaagatg ttgaggtgca agcagatcca tgtaaatggt attatgataa gcatggaata
     2341 atttcacaaa tagactgtat ggtggattgt gcaggatcaa ctccaaaagg aacagtagct
     2401 tattaaaaaa tactaatcag tctattaaat atgttgaaag tactagatgt agttttttac
     2461 aaatgaaatg tgattttctc aactctagta tttttaataa ttaaatttaa ctagcacaac
     2521 gagttagtta gtatttaatg aatagtgctc tataacctac tattttaggt tatagagttt
     2581 ttcttgtaaa tatgaataag gaggattctg attatgataa atattaaact tgcggtgtca
     2641 tatttaaaga gacaaaaagg aaaaacttta tcattaatac ttagtatagg gattgctgtt
     2701 atgctagttt tttcattaag tgttattaat gaatctcaag gacaatcaga tataaatcat
     2761 gcatacaaaa actcgggaga ttatcatgct ttttatgaag atataaatag tgatatagta
     2821 aaagaactta atggtgaaga agatataaaa gaattcaatg atgtacttaa atttggagaa
     2881 attataagta aagataatgg agcatctcta aatttatgct cttacaataa agatttttta
     2941 gattctacta gatataagtt agttaaaggt agagagccta gaacttctaa tgaattagta
     3001 atagaacaaa aagttttaga caaaatggaa ttagatgtca aattaaatca aactattgat
     3061 tttcagattg ttaatgagca tataaataaa aataatgaaa attctattta tagtaaagat
     3121 aagcaattta aaatagttgg tattttaagt aaatctgatg aatactatga aaatttaaat
     3181 aaatataagg taagagcttt tacatattca aacaatggca aagatattgt tccatatgaa
     3241 ttaataactc atatgggtac attaaaattg aatacaaaag atttagattc tcaaaaagta
     3301 gtgaatctag ggagaaaata taagttaaca agtaatcaat tttatccaaa tacagaagta
     3361 agtatgtcta tgtatatgaa aaatatttca aaaaaaacaa cttattcaat aaagcaaagc
     3421 ttactccctt tattatgtgc aacattagta atatacaata tgtttaacat aataatacta
     3481 gatatgacca agcaaattgg tttacttaga gctgttggag ctagcaagag aaatattaga
     3541 caggtatttt ttattcagag tattgtagtt ttatctgtag gtatagtaat tggtttactt
     3601 ggaggtatag ttttttctta cttaggtctc atgatagtat atgataaaag tgcagaatta
     3661 actataaata caaactctat aattgaatct atcatcatgt caattatagc agttatatta
     3721 gcctctgtag taccaatata taaagctgga aatttatcta taattgatgc tataaaaaag
     3781 acagataagt taaattatat tcctaggctt ttgttgttaa aatcacataa aaaagttgtt
     3841 ggaattataa atgagatagc ttttaaaaat atatttagga ataaggttac atcaataata
     3901 ataataatta cgatatcact atgtggggtt ttatttatag gcagactaac ttctacaaaa
     3961 ttcatatatg gaaaagagtc agattcagct aatataacaa gtaaatcata tggcaatttt
     4021 gatatttatt tgggatattc accaataaat gcaaattata cattttctaa atatgataat
     4081 tctttattaa atgaaataaa agctattgaa gatgtaacag atgttgaacc caatatttat
     4141 ttaaaaggat atctaagaaa tgctgactta gaagaagatt atttagatga gctaaaaaga
     4201 actgaaataa ataataataa tgagtctaca gttttaattc gaggttacaa taaagagcta
     4261 ttaaataata tagataaata tatagataaa ggcgaaaatg tttatagtta tacagacagg
     4321 gattataaaa atgttttatt ggtaaataat ttttattcta gaattaaatt gtcaaataat
     4381 actcagatta taaaatcacc taaaatagga gacttaatag atataaaaat tcctgtatat
     4441 aaagatggag agtataaata tataaataca aaagtaagaa tcgctggaat aatgaaaaat
     4501 agctatatat cagagcaaga tggagaatct caatcaggtg gaattcaaat tatatttaat
     4561 gaaaaggatt taaaagaatt aacagggata agtgattata ataaagtttt tgtgaagata
     4621 aaaaaagaaa cagataaaaa agtaataaaa gaaataaata atataataga aaaaagtgga
     4681 ttttcagata tagagggaag atatgtacgt aatcattttg gagaccatta taataaatcc
     4741 tcatataaat tagcaatgat ttgtgtattt ggagttttat taatatcatc tataaacatt
     4801 atttttatag ttagatcaaa tataattgtg aggttgagtg agatatgtac atttagagca
     4861 atagggatgt ccaaaaaaaa tgtaaaaaga atgttaataa aagaaaatat gatatatggg
     4921 gtattaagta tgattgtagc tggaattata tctagtttca attattataa aatagtttct
     4981 gaaactaaca aacttaatca acaggtatat ggtataaata actctattaa atttgaaatt
     5041 ccaatatatg aaattctaat atttggatct atatctgttt ttgtttgtgt cattgcagtt
     5101 tatttttcta ataaaatgat aaataaactt agtatagtaa gtggtataaa agaaaattaa
     5161 tgaaatttct aatttaaata tataggaagg aaataaaata aatgaatagc tcaaacaaat
     5221 attttttaga ttgtatatgt gaaaaaataa taaataataa tcaagttatt cttagtaata
     5281 aagtaaatgg aagatgggtt aaaattccaa tagaatgtta tgaagttcta aagtattcag
     5341 tagataatgc attacaactt ggtaatttgt atgatgtatt ccaagaaaat gaagataaaa
     5401 aatattatga acgagttata gataagttag agcatatagg acttctaagt gattctattc
     5461 taagtgaatt tagactagag agaataaaca aaatttcatt ttctccaact aatagatgta
     5521 atctaagttg tgattattgc tgtgtagatt ctaaaataga taataacgat tatttagata
     5581 catctcaagt aagaaaagta atagataata tagttaaatt aaatccaagg atattaacca
     5641 ttacaggtgg agagccattg atgagaaatg atttcttaga gctgttggat tatataaaag
     5701 aaaaatttaa aggtaaaata attctgtcta caaatgcaac tttaattaaa gaaaaacaga
     5761 ttgatgatat aattaatggt ttatatgcaa tagaaattag tttagatggt tatgatgaat
     5821 taagttgtag tgaagtaaga ggtaaaggag tattctgtaa agtattaaat gtgattagac
     5881 ttataaaact aaaaaaattc gagaacgtag gtttgtctat ggtggtagga aagaataatg
     5941 agaaagacat agagaaattc aataaattaa atgaagattt aggtacaacg cctataataa
     6001 gaaattttat gaatattggt agaggtaatg agaactatag caaatatctt aatgataaac
     6061 ttgatattga ttacatttgc aaagatgatt atgtggatga aagtagttta tgttcaaata
     6121 tgtgtaaagc aggtgttaat cagataagta tagatcataa aggagatgta tatccgtgtc
     6181 caaacttaga gtataaggat ttaaagatat tcaatatatt agaatttaat gaaaacatat
     6241 tagagaggat tcttaataga gaaatggata tatttaatat atttgataaa ttaaaaccag
     6301 taaatattga gagttgtaaa gaatgtaaag taaatttatt ttgtacaact tgtccatcga
     6361 aaatatatac tttaaaaaat aacaaaaaaa tgtttgatgc aaattgtaaa aaaatgaaaa
     6421 acatcttgga acctatatta tggtaaggat tagttttata ttcaaggtaa tttattttag
     6481 aggtgattat taattgacta aagtaagaat agcagtctta gacacaggat tagatgtaaa
     6541 tgatgatgat attaatggaa attatgttta tgattcaaat tttcaattga aagatttggg
     6601 ggggaaggaa ataaatgaca taaatggaca tggaactttt gtatcgaaaa ccatattaaa
     6661 tataagtaaa aatacccaaa tttatcctat taatatattt aatgattctg gaaaaacaag
     6721 ttctttaaat cttcttagta cactgagcaa gttgactatg tcggatatac agataataaa
     6781 catttcagct gcatctatta atagtatgta tgaaaaagaa ttagaaaagg tttgtaatat
     6841 tttaaatagt caaggtagat ttattatttg ttcaaaacat aataaatatg aagataaaga
     6901 aagtattcct actatatttg aaagtgttat tggtgtagta ggtaatgaaa atatatataa
     6961 aaataatcaa tatatatacg aaccagaaaa taagattcaa atgtatgcaa attcaaaaga
     7021 taattttatt aaatttaaag ataaagtaac tcattttggt aaaaatagta aggcttgtgc
     7081 agtggcaact ggcattgttg caaatatatt aaaaaacaat ccatatatta agtttaatga
     7141 attggaagaa atacttattc aaaaaagcaa aagtaaagaa gatttaaaaa tagaaaactt
     7201 gaacactcca agttataaat ctaatttgat aagattggat atagcaagga aaataataaa
     7261 tgtgataaat agaaactttt ctaaaataaa aatagacttt gaatttgtaa gtgaatattc
     7321 tttatttaac aattttacat gtattggaaa ttataatgca tataattttt taactatttt
     7381 aaataaagag tttaatatta gtattgacta caaagaagta tacttatatc aattagagaa
     7441 tttagatatg gcagtagatc ttatttacga aaatttaatt taaaaatata tgttttttaa
     7501 atgggaatgg agatacgtta tgaaaatact taaggtaaaa gatttaaaaa aaatatatgg
     7561 aaaatatgag aatgaagtta gagctcttga tggtattgat ttagatatag agccacataa
     7621 atttacagct attgtaggtg caagtggttc tggtaaaagt acactacttc actgtgttgc
     7681 aggcttagac aagccaactt tagggaatgt gtacttagat gatgtagatt tatatacatt
     7741 aaatgatgat aagctgtctg aaataagaag acaggaattt ggcttcatat ttcaaagtta
     7801 caatttaata ccagttataa atgtatatga taatatagta cttcctgtgt tgcttgatgg
     7861 gaaaaaagaa gataaagaat atgttatgag attaattaat aagttgggga ttgaaaaaca
     7921 aataaagaaa tatccgaatg aactatcagg aggtcaacaa caaagagttg caatagctag
     7981 agctttagcc aatagaccgt cagtaatatt tgcagatgaa cctactggca acttagatag
     8041 taagactaca aatgaagtta tgaatatgct tgaatggtgt gttaaagagt acaaacaaac
     8101 actagttatg ataactcata atgataatat tgcaaagaca gctgatagaa ttataactat
     8161 aaaagatgga aaaatagatg aaaaaaacta ttaataataa tttatttaga atactaaata
     8221 taatagtata taagaataag actaagttga tagtatcatt tataatggtt tttataattg
     8281 ctggcgtaaa tattgtaata cctcaactaa ttagatgtat aatagatgat ggaataggaa
     8341 ataaaaatat ttttctgctt ataaatttaa tattgatata tatttttata aggatattat
     8401 cagctacatt tgatgttata ttatcatatt tatatacttc aataaaaaat aaagttacta
     8461 taaatctaaa agtaaagttg cttaaccata tatcaaaact ttctggtaga tattattcaa
     8521 gtaagaaaac aggtaatatt ttatccatta tggaaagtga tatatttatg atagaaaact
     8581 ttggagcaga gattatattt gatattattg taaatgtgtt tacagctata atatcgttaa
     8641 ttttcttaat taatttgcag tttgatttat ttttagtagt tttattttta cagttgatat
     8701 taacattttc tcaaaataaa atgacaaaac ttatatctaa gaaaatttct gaaataagaa
     8761 gtgattcagg cagtttatca aatttgattc aagagtacgt atctaatatt atggatattg
     8821 ttatattaaa ttcaaaaaag aatttcttta ataagtattt aaagagagag agagatttga
     8881 taaaaaaaga tttgaatttg aacatcatta tgagtagtaa tatagcaata tctcaaatac
     8941 taacaggatt gataacaata ttaatatatg gaattggggg atataaaata ataaataata
     9001 agatgacgat gggggagtta atagcatttc aacaatatac aggaatgtta attggaccat
     9061 gtatagctat agtaagggcg aatactagaa tagcacaatc aaaagtgtct atcaatagag
     9121 tattttcagt aatagatgaa aagatagata ttaaagttga taataatgga tatagaatag
     9181 ataacagtga aattaagaaa atagatttta aaaatgtatc ttttagatat ggtgaggatt
     9241 taaaccttgt tttaaatgat ataaatttac aatttgagaa aggggaagta acaggaattg
     9301 ttggtggaag tggatgtggc aaatcaacaa taacaaatct aatataccga ctttgggata
     9361 taaatgaagg tgatatatta atagatggtg ttaatataaa ggatattaat ttaaaatcac
     9421 taagaaataa tattgatata gtaactcaag atcaatttgt atttgatgac acaataaaaa
     9481 ataatatagt attaaataat aaaataagtg ataaagaatt agatgagata ttagaagtta
     9541 cttgtttaga tgattttata aaaaatctag ataagggatt ggacgaagta gtaggagaaa
     9601 aaggtgtcaa aatatcagga ggtcaaaaac aacgtatatc aatagctaga gctttagtaa
     9661 gtaataaaaa ggtacttatt ctggacgaag caacttcagc tctagataat atttcacaaa
     9721 aagagatatt gaacaacttg aaagagtaca tacaagataa gatagttatt ataatagctc
     9781 atagactttc aacaattaag agtgctgata atatatatgt attagataaa ggtagagtaa
     9841 tagaatctgg aaatgatgct gaacttctac ataaagatgg tttatataat aagttagttg
     9901 aaactgcaga ttttgcataa gcatgacttt gtatgtacat atatgattat tagggtattt
     9961 aatggaatct ataagttata taactaattt agttatataa aagtttgcaa atcaatctca
    10021 gataatgtta aacaaatgat tagtatatcc agtagaaaaa ttataatagt agtagaatat
    10081 attacaaata aatcaaaact tcacataatt ttcatattaa ttatcaatta aattcacaca
    10141 caagagttat tatataaaca tagaagatag taaaactagc ttaaaaacat aatataatac
    10201 cctatacaaa atagtggcaa ctctccctca catcctcaat tgttgccact attttaattt
    10261 tttgtaaaaa acaaaaagac tccacaaagt ggagtcttta taaatttaac ataccctttt
    10321 aataatatgc tttactatgc tttaaaacaa acagaatttt taatctcaag ttttttattt
    10381 atcttagcaa ccttaatctt aatcttatcc ttcaaaggaa tacatcctat attactttca
    10441 ctttttacat caacacttcc aacaaattta ccagagtcat tataatactt tgcaacacca
    10501 acaatatctc ccttatggat aggataattc atatttttaa acactacctt aggattgata
    10561 ttttcgttat ctgaaacaac accatacaaa tctttatcaa ccttaactgt tatcttaaaa
    10621 tcatcaatac catcaacaca aggagtctct ataatatcac ccttatgtgc tatcttttta
    10681 gaatggaaat tatctttacc atatttcaat aaagtagcgg aagaagaaat tctatttttc
    10741 ttgttaccat caccaagtac aacacctatc aatctatgat ttctttcgtt tttagcatct
    10801 ttaggtacca tcatagaaaa tgcaaggcaa tatccagcat tatctgtata tcctgtcttt
    10861 attccatcaa caccaggtac tgaaacaagc agaggatttg tgttataatg tgtaaaatcc
    10921 tttttagtat cattatatac ttgcatagtg gttattcttg taacttgatt ttcataatgg
    10981 tcgtacatat actttccaag tgtaactata tcgtgagcag ttgacatatt ttcaatagga
    11041 ggttctttat gttctgggtc agtatatata ggaagcccat ttggattaaa ataatacgta
    11101 tcaatcattc ctatttcttc tgcttttttg ttcatcatat ttacaaaatt cttttcagtt
    11161 ttaccaatat gtttagctat ggctatagca gcatcatttc cagatactag cattagaccc
    11221 tgcataagct ctcctagaga tatttcatca ccatctttaa gtttacaagt agaacctcct
    11281 actgaagctg tagacttgtc tattttcacc atatcagtct ctttaacttg atttttatca
    11341 actgcttcta ttgccaataa aaaggtcatc attttactta aactcgctaa tggcattttt
    11401 tcatcaatgt ttttttcaaa taatattctt ttagtttcct ggtctataag tagtgctgct
    11461 cttgaagaat ttggtactgg tggagtatct gcaaatgcaa atacaggagt tataaatata
    11521 aaaattaatg caagtattgt taactgtttt atttttcctt tcaaaatctc gtcctccatt
    11581 tcatatatta attaatatgc acaaaataag tgctataaat tttatttatt tagatactca
    11641 ttaatcatgt ctattatata agcaatttct tcgtctggta tcaaaatatt aaaataagct
    11701 tctgttggct taaaggcttc acgtattaaa ctatatagtt catgattttt actaattaat
    11761 tcagtaatgt tattatatgg aaaagttgta tttttaaaac atctttctat cataaaagag
    11821 caatgtagta tatatcctag cttaaatttg ctctttaagt tttcatctat taattctatt
    11881 attgtaaata aggaagaatt gacatagttg aataatttaa cagagtccaa aagctctaag
    11941 ttttgggtca taaagtttat tattaaactg ttgtatgata gatttgtttc ttttatattt
    12001 gtaatactat tggcattgaa tgtaccttct attattttgt ttagcatatc taggccatat
    12061 ccatttagta gttcctctat gtgtataaaa ggggtgttgg ctacattaaa atctacactt
    12121 ccaacaactg ccagaatttt agtattacta aatttattct tatacatttt aaaactttct
    12181 atattatgag gaactaaatt aatattgtat ttactaatat ttttaagtga agaatttata
    12241 aagtctgcta ctttcatagc tgaaccatat cctgttatac aagtagttat tattacatac
    12301 tctcttgact tttcaggata gtttatttct gttgaatcga ttgctgctcg tccaatatat
    12361 ggatttaaag agcttaagga attagctaat tcatccaaag tcatttctgg tagtgtactt
    12421 tttcttactg cttctaaaac tattggagta gataccattt caattgagcg agttttgatt
    12481 tttgtttttt ctgaaataag ttctccaaaa gctataagag agcccatatc tgttaaaaga
    12541 agtacaccct taccttcgtc acattctttt accaactcta tagttttatc aagtatggta
    12601 cttactttta catcaagagg catgtcaatt gcattacaat ggtttgtacc aagcaatgaa
    12661 tttgctacat cacacatact gcttgctgtt gaatttccat gagcaagtat tatgactcct
    12721 atactttcag attgattgtt tggttcaaga tacatgctat ttagaaacat ggatataaat
    12781 tttacttcat ctttaggtaa agatgtttgt aagtcatttt caattatact taaaactatt
    12841 ttagctatat ttaaatcttt atctaagaaa attttatcat tatataaatt gtacttagga
    12901 ttatatgtgt cttgatttat attttctata agtgcactta tatgcattga aagagcaata
    12961 taagtttgtt ttgtgtattt gtttctcgat attttttcag cctcttttaa agaatgttca
    13021 acagcatagt aaatctttgg attgattact ttaaaaatct cattacttgt ataatcagaa
    13081 tgagttactg aagatatcga aagtaatttt gtagagtact tttccatgta atttataaac
    13141 atactcctta tatcattttc cagataagga atctctgtag agtatttaaa tccattgttt
    13201 gatgtatcta tattgtattt agatagttct tttgtatatt caacttctaa gttattacca
    13261 ttaaattcat aaaaaagcat ataatcaagt tctgataatt tatttagatt actacgattt
    13321 tcattcaatt ttaaaagacc aatatagatg taatcaggaa caagggataa atctatttct
    13381 atattatcct taagtccagt tttgtaattt aagaaggctc ttgcactcat aagttgtatg
    13441 tcagatttaa gctgacctat attaccaaaa caatcataaa ggagtaatga tttaattaca
    13501 tctttgtgaa cttttatagt tgcattggtg tattttgatt ctataataaa gaagtattta
    13561 ataagagata atctttcttt taaagaccta tcctgtaaag caggtaggtt gattgtaaca
    13621 gggattcttc ttaaaaaagt cccaagcaga gaagaatgta tatcttctgt tgtggcacac
    13681 acaataaaga cattagcttt gtgcataatg tcagattctc ctaatcgtct gtatatgcct
    13741 ttgtccatca aaagaaaaag catctcttga ccttctggtg gcaatctgtg tatttcatct
    13801 aagaataata tgcctccatc agcctgtgca actatgccga atttatcctt attagcacca
    13861 gtaaaagaac ctttgacata accaaataaa ttacttaata ataagttagc attttctgca
    13921 tattcagcac agttaaatac tacgaatttg ctttcagaag aaaagatttt actttctaca
    13981 gcatatttgt acattgtttc agcaaatgta ctttttccaa caccagtagg tcctattagt
    14041 aacgtattaa gaccttttgg aggatagagt atagcagatt tagctttttc tataggtagt
    14101 ttaagactag aatcatgtcc aagtaatgtt gaaaaaatat cttctgagtc atttttactt
    14161 gatttaagta gctcattaca attatttata gaaaattcat cactgttcaa agtacggcca
    14221 attaaatttt ctataatact agcatgtaga aataaaattg gcttaccaga tattttcaga
    14281 actttttttt cattgtatag gttatttaat tctttactgg tattacttct atcaagagat
    14341 gaatggttag caatttctat tgtagtgaat cctgttttat ctgattgaag gtcttttaaa
    14401 gtcatatttt cacataattt atgtagagat aatagaactt tatcttttct tagcatatac
    14461 cgtttccttt ctttatgttt aaactatatt taagctacat taaatatatt ataatatata
    14521 ttcagtatta aaataaggtt tatagtggat attttgtatt ttacttttta atatgttgtt
    14581 ttgt
//
LOCUS       DAMJYX010000083        14572 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMJYX010000083 DAMJYX010000000
VERSION     DAMJYX010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.085"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            33..704
                     /locus_tag="PC479_002693"
     CDS             33..704
                     /locus_tag="PC479_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805228.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            706..1614
                     /locus_tag="PC479_002694"
     CDS             706..1614
                     /locus_tag="PC479_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2805229.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1691..2377
                     /locus_tag="PC479_002695"
     CDS             1691..2377
                     /locus_tag="PC479_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805230.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2374..4929
                     /locus_tag="PC479_002696"
     CDS             2374..4929
                     /locus_tag="PC479_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2805231.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5123..6322)
                     /locus_tag="PC479_002697"
     CDS             complement(5123..6322)
                     /locus_tag="PC479_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2805232.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6441..7328
                     /locus_tag="PC479_002698"
     CDS             6441..7328
                     /locus_tag="PC479_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2805233.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7518..8075
                     /locus_tag="PC479_002699"
     CDS             7518..8075
                     /locus_tag="PC479_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805234.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8314..9174)
                     /locus_tag="PC479_002700"
     CDS             complement(8314..9174)
                     /locus_tag="PC479_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2805235.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            9673..11076
                     /locus_tag="PC479_002701"
     CDS             9673..11076
                     /locus_tag="PC479_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2805236.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHKSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            11295..13772
                     /locus_tag="PC479_002702"
     CDS             11295..13772
                     /locus_tag="PC479_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2805237.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            13787..14473
                     /locus_tag="PC479_002703"
     CDS             13787..14473
                     /locus_tag="PC479_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805238.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
BASE COUNT         5649 a         1690 c         2204 g         5029 t
ORIGIN      
        1 tatttatcta aaaaatctaa aggagagtaa aattgaactt attaattatt gaagatgata
       61 taaacttaaa tgaaggtctt ttttatgcat ttgagaatga tggatttaat gtatttaagg
      121 cttatacaaa acaagaagga ttaaatatat ttaatagtaa aaatatagat tttataatct
      181 tagactgcaa tcttccagat ggagatggat ttgatgtatg tcaaataata agggaaaagt
      241 cagatatacc aattataatg cttactgcaa gagactcaga aattgatgaa gtaaaaggat
      301 tagaaatagg gcttgatgat tacattacaa aaccattttc tttatctgta ttaaaggcaa
      361 gagtcaaagt agcaataaga aaaaaatcga ataataaagt aatatattcc aatggaataa
      421 aattggacca aaagttacta aaagtatata aaaataaaga atgtttggaa cttagctctg
      481 tagaatataa gcttctaagt tatctaattg aaaataaagg tcaaatatta ttaaaggaac
      541 aaattttaca ccatatttgg gacagcgaag aaaattatgt tgatgataat attgtatctg
      601 taaatataag aagacttaga gtaaaagtag aggatgaccc atctaatcca aaatatataa
      661 aaacagctta tggaatgggt tatttatgga atgaggtaga ataatatgtt tatattagcc
      721 tttataatat gtacagttgt tatatttata gtatctacta aaataaatcg aaaaagatat
      781 gatgaattaa atttgatgtt aaatcaaata ttggaaggta aagaagttac ttatccagat
      841 acaaaggata caagagcgtc aaaaatttct caccaagtaa aaaaaattaa ggatatgata
      901 gaaatagagg tagaacagtc aaagtcagaa aaagaagcga ttaaaggttt aatatcaaat
      961 atgtcacatc aattaaaaac tccactttca aatataacca tttattgtga acttctggag
     1021 aatataaata tttccacatt acagaagaag gagtttttac aaaagatgaa aaatgaaact
     1081 tttaagattg actggctgtt acaatcctta tttaaaatga ctaaattaga ggatggagtt
     1141 atagaatttg aagtagaaga actactaatt aaagatactc ttattcaaag tatcagtact
     1201 atctttaata aagcagaagc taaaaatata aggataaatc tcgaaccatt ttcagacata
     1261 aaattagttc ataataaaaa gtggaccata gaagcaattg taaatgtttt agaaaatgca
     1321 ataaaatact caccatcaga tagcacgatt actatttctg taataaaaat ggaactatat
     1381 acgaagatta ctattaaaga tgagggaata ggtattgata gtagagaact aaatgatatt
     1441 tttaaaagat tttacagaag taaaaatgtt gaaaaccaaa atggcacagg gattggtcta
     1501 tatcttacaa gattaatttt agaaaaagaa aatggaaaca ttatagtaga atctaaacta
     1561 ggaagtggat gttgttttag tatcttctta cagaattgta agagtttaaa ctaattttgt
     1621 aggatgagtg taattatcgt cacttacaat attttataga acatatatct gtattgaaag
     1681 gagaaattaa atgagtatat tagtgacgaa taatttagta aaacactatg gaaaaggtga
     1741 gactaaagta aaagccttaa atggagtaag tatagaaatt gaacgagata catttactgc
     1801 tatagttgga acttctggtt ctggaaaaag cacattgtta aatattatag gtggattaga
     1861 taatccaacc tcaggtgatg taataattaa aggaaagaac atatctaaaa taggaaaaaa
     1921 agatttgaca gtgtttagaa gaagaaatat aggatttata tttcaaaact acagtttaat
     1981 gcccgtatta aacacttatg ataatatagc tcttcctgtt acattagata aaggaaacca
     2041 tatagaccat gaatatattg aaatgctgat gaagacattg ggaatttggg ataagcgttt
     2101 aaaatttcca agcgaactat ctggtggtca acaacagagg gtagcaattg ctagagcttt
     2161 ggcaaataaa cctgcgctta tattagcaga tgaaccaaca ggaaatctcg atagtaaaac
     2221 tacaatggaa gtggtatgtt tattaaaaga aagtagtgca aaattccatc aaacgatact
     2281 tatggtaaca cacaatgaaa atatagcaca agtatgcgac tcaattatcc atattgaaga
     2341 tggagttgtg gtgaatactg gtggtgaagt actatgatgt ctctagtaaa attttcaatt
     2401 caatatataa agcattataa aaaacaatct atatctatag ttttaagtat agtactatca
     2461 gtagcattat tgacgggaat tggttcttta gttcatagtg cagacaaaag tagaattgaa
     2521 aagataaggg aagatagtgg agattatcat tatttctata aagtagataa ggaacaactt
     2581 aataaaatta aaaaaaataa aaaatcaaag gaatacactg tagatagact tggcgttaca
     2641 aaacctaaaa atcagataga tgaacctttt attatagaat ttttaaatgc tgattcttca
     2701 tatttagaca tgacaggtag aaaattatta aaaggaactc ttcctagtaa agatggagag
     2761 atagtacttg acacatattc tctaaacaat ttaaatgtaa acaaagaaat tggaacagaa
     2821 atcaatttag atggtaaaat ctataagtta tgtggtattt tatcaagtct ttcaaaccca
     2881 gaaactgctc tggagggatt tgtaaacgat tattttcagc ctaaagaaaa ggattcttat
     2941 atagtttatg taaaatatga tgaaaataaa aatattaaaa agcaaaatta taaatttatg
     3001 aaaaaattca acatatcaaa agaaaaaact tatataaact ggcctctttc agttgaattt
     3061 ggagtaaaac caccaaaaaa tatagaaaaa tataatatat ttgagttctt aaaaaattta
     3121 gaactaaata caaatgcaat tatattactt gttggagtgt ttggtgcagc agcagtatat
     3181 agtattttcc atgtttcaat acttcagaga atatcacaat atggggtttt agaagtatta
     3241 ggcgctaata ataaacaact tttatttcta ctatttttag agttattttt actttttatt
     3301 gttggatttc ctataggttg cttcttaggt attgaagtgg cgtctacaat atatgaacaa
     3361 tttccacata tatttttgag tagtgatatt gttccaggag cattttttat aagtgaaaaa
     3421 tctatttatt taggcttttt atttatctta ttgttgttaa ttttaattgc tgtacgtgtt
     3481 gtacatcagt tgaataaata ctctagtata gaatctatga aaaactttaa gattactaat
     3541 aagaaaaaga gaatattaga aagtgtaaaa cactctaaca tgacaaaagt attatctcat
     3601 aaatacatga ctcaaaaaaa gggaatgttt attggaattt tgttttcatt atcacttggt
     3661 ggaattatat ttttatgttc tagttactct atacagctta ctaaaaataa taatgaactt
     3721 acaatgaaag ctgatgatgg tttaaattct gactaccaaa taactatgca aacaacagat
     3781 tttgatgtag gtataccaaa agataaaatt tcaagattaa acaatgttga gggaatatca
     3841 aaagtctatc cagtaagata tttatttgga gctatgcaaa ttaaagaaga acaactattt
     3901 tggaaaaatt tctttaaacc tcttgaaaaa gattacagga taaagacttt ttttgatggt
     3961 atttgtacaa agcaaaatga aggtggctat cttttaaaaa caaatattta tggttataac
     4021 aatggtatgt tagaaggatt aaattcttat attatagatg gggagattaa tacccatgat
     4081 atggttaaaa ataataaggt tatagttaga ctacctatgg atggaactgg gatgtatgat
     4141 gcagttgata taaatccagg agatacaata aaagttaaag tgccaaagac aatgaaacca
     4201 acagatgaaa ttgttaaatt taaagaagaa aataagaatg attatacaac aaaagaattt
     4261 gtagttgctg ctactgtaaa acgtgtgatg gcaaataaca tatatttcat tggtgattat
     4321 ggtatggata tagttatgac gaatgggcaa atgaaaaata actttgatat cgaaaattat
     4381 aattcagtaa gtataaaaaa gacagaagat agtaacagtg aggaattgtc tgagaacata
     4441 aaaagtgttg taagtgatgt aaaaagatgt atcgtaacag attataccat tgctattgaa
     4501 aaaaataata cctacttaaa tcaaaaactc ttatttatat atggtattgt atttgtactt
     4561 cttgcaataa gtttgttcca tataatcaat acagtaagct acttaatttt ttcaagaaga
     4621 catgagtttg gaattttaag agctatgggt attacggata ataagttttt acttatgatg
     4681 ataagagaag gttttttata tggattctat gcaagtatta taatggtaat tggaagtgtt
     4741 attggacaat ttatgattta ttttatggta aaacgtgtat atttatatat aaatccaatt
     4801 cttaaaataa atacacctct atacataggt atgattattt taaatatcac tattagtata
     4861 gttgctgtaa ttattccagt aagacaaatt ttgaagagtg atattatttc agaaataaat
     4921 aagaattaga gttatataaa tttttaagat tatataactt tctgacaatc ttgtttaaat
     4981 tatttaaata tatcttatct gtgaacatta aataaacctc ttaatagata taactcttga
     5041 aaagtcatat ctattaagag gttttaataa attttcatgt taatatatat aatccaaaaa
     5101 aattaaatct tattaaaaca tactattttg tatagttatc gaaatatcct tgtatgtaaa
     5161 taataggagt acccttatct ccacttccag atgttaaatc tgctaaagaa cctataaggt
     5221 ctgttaatct tcttggagta gtaccttgag aaaccatgtt tcctgttaaa tcatcagatt
     5281 tattatcttt ttcaactatg tattttgaaa ttgcttcttt taactcatca ccagataagt
     5341 cagcaaaatc attatctgct aagtatttta attttacttc gtttggagtt ccctctagcc
     5401 cttttgtata agcaggagat actactgggt cagctaattc ccatatttta cctacagggt
     5461 ctttaaaagc tccatctcca taaaccatta cttctacatc tttccctgtt atttctttta
     5521 tattaccttg gattttgttt acaacttcat cacaatttat agggaataat tttactgttt
     5581 cttctgtagc tttgtttgaa cctaaaagtc catactggtc attgtaacca ctaccatcta
     5641 cacttgctgt cattatatca tcaagtgaat atactttttt agctccattt tgtcttaaaa
     5701 ttctctttgt tctttgtctt gtatgtatat cacaagttaa aatacttgtt gtatagttaa
     5761 gaatagtttt tggattgttt gcaaatacta tttctacctc tgcaccacaa tccttgatta
     5821 aattcttata ataatcaaca taatctacac cagtaaatgt atgcttgtta tatccaaata
     5881 gttctctgta tttttcttct gttaaaacat ctgaccaagg atttattccc ttatcatcaa
     5941 gttcatcaat acttattaag tgatttccaa cttcatcaga agggtagctt aacattaata
     6001 cgacttttct acatcctttt gcaattcctt ttaaacatat agcaaaacga tttctactta
     6061 aaattgggaa tataactcca acagtatcat ctccaaattt atcttttaca tcttttgcaa
     6121 tattgtcaac gtgagcataa tttccctgtg ctctagctac aacagcttct gtaacggcaa
     6181 ttacatcttt gtccctaact tcaaagtttt cactttttga cgcatttaaa acagaatcaa
     6241 caactatttt aactaagtca tctccttgtc ttataatagg tgctctaacc cctcttgaaa
     6301 cagttccaac taatctatcc attatctaaa atcctcctaa agaattaata ttgtcatatt
     6361 agtaccatgt atattataat tcaaatataa ttataagtaa aataaataat actaatattt
     6421 gacataagga gtaattatat atgaatataa aacttgaact ttataaggtt tttaatgcag
     6481 ttgtaaataa taagagtttt tctcttgctg ctaaggaatt gtttatgtca cagccagctg
     6541 taagccagtc aataaagcaa ttagaagaac aattagatac tttattattt tatagaaaca
     6601 ataaaggagt aaaattaact ccagagggga aaattctaag tgaacatgtc actactgcat
     6661 taaagttaat atcatcagga gaagatagaa taaataaatt taaaaaatta gaatacggtt
     6721 ctctgaaaat aggagtagga gatacagctg cacgtttttt cttgttaaaa tatttagaaa
     6781 tttttcataa gaagtatcca catatacatg tatctacaat aaatagaacg agtagagaac
     6841 ttatatcttt actcaaagat gggaatatag atatagcaat tataaacatg ccaatagaag
     6901 atgatacact aaatattgtt gagtgtattg aaatacatga tatatttgta tgtgcaaatg
     6961 attacataga atacaaaggt aggaaaatat cattggaaga attaaacaca ttgcctttaa
     7021 taatgctaga aaataaagca aattcaagac tttatgtaaa tgaatatttt ttatcaaaag
     7081 gtataaaact aaacccagat atagaattgg gttctcatga attactttta gaatttgcgt
     7141 atataaatct tggagtgtct tgtgttatag aagaatttag catagattat ttggaaaatg
     7201 agaaattgtt taaattagat ataaaagagc caatacctaa gagaaatata ggttattgcc
     7261 atttaaaaga tatatcttta tccttagcaa ccaaagaatt tttaagcatg atatctaata
     7321 atatataatt tagaatacat atttcacaag taatttaata taaaaataga gataaattcg
     7381 taaatttgaa agattcatat agtaattagt ttttattaaa aataaaaata aggttaattt
     7441 gagaacattt atataattaa tatcgtattt taaatatgaa cagtatattt agattaatgt
     7501 ataaaggggg attagaaatg tctgattctg aatatcctca aatgaaaacc ttagaaacta
     7561 gaagttgtat actaagacct gcatcattag atgatgttga agattttttt gactgttata
     7621 aaaataaaat agtagtaaaa catttaccat ttagtgagca taaatcttta gaagatacaa
     7681 gaaagtttat aaaatcattt tttctaaatc catataaaaa agggaaaatt ggccactttg
     7741 ctatagtata taaaagagac aataaagtta ttgggaatat ggggtttaat aatataaatc
     7801 ctaaagcatt agaagcagaa ataggtatat gtataaatcc aagctattgg ggacatgatt
     7861 ttgctactga gataacaaaa agagtaatac agtatggatt tagggaacta aacttaaata
     7921 aaataattgc aataacctat gaagaaaacc aaaattctac aaaatcatta gatttattag
     7981 gatttaaatg tacaggaaag tatatcaaaa aaattcatac tggaaatact gtaaaaaatg
     8041 ttccttgtta tcaatatgaa ttgaaaaaac aatagatata atcatttaat atctatgctg
     8101 gttatttagt aatgattgtt ttctaaataa aatttcaata aagtggttgc ctcaaaatat
     8161 agataatcct gtttttattg ttaaaatact gaaaatgcat ttgattttat acaaaaaaga
     8221 gtgtctcatg aactaaaaag tttatgagac actctttttg aataaatcgt attttaatta
     8281 aaatagtata tatacttaaa atattaattt ttattagttc tctaaatttt taaatccttc
     8341 tccaagaacc tcatgtacat ctgtaacaat aacaaatgca gtagggtcag ttcttttaac
     8401 taatctcttt agataaagtt cttgtttttt agaaactacg actaatagaa cttctttatt
     8461 ttctctagtg tatccacctt taccatcaag aatagtaaga cctctatcaa gttcttcagt
     8521 tatagcttgt cttaaacttt caggctcatc agagataata aaaaatgctt ttgaatggtc
     8581 aaatccttcc attatcatat ctgctatttt tattaataca caaagtgcta taccagagta
     8641 aagagcagtt tctaaactac gatttactat accagaagat ataacaacca ttccatctag
     8701 gcatgacatg aattttgtag cttttattcc tgggaatttc ttactaagta tgagtgcaat
     8761 taagtctgtt cctccagtag aagcatctga tttaaacatt attcctaaac ctataccaac
     8821 taggattcct cctgcaatag aagacaataa taagtcatta gtaaatctta gttttgataa
     8881 tgggtctgtt aattgaacta tagctgaaaa tacaactgtt ccaaataaag tttttaatga
     8941 attttttgta cccataattc taaaagcaag tattactaat gggacaccta taattaacat
     9001 gatagctgat actggaagtc ctgtaacctt gtttagtact aatgatagac cactaagtcc
     9061 tccaggagca attgtatgtg gttttaaaaa catatttata ccaatactca ttgatataca
     9121 accaaaaaat agtccaagca tttcaagtaa aattggcgac ttttgttttt tcatataaac
     9181 acctccatga acttgtcaca atttaatatt ataacatatt tgaccagttt atattatata
     9241 aaatttcttg atataagcaa tttcaagtat ttcattattc ttaatatttt tgaattttta
     9301 tatagtgttt tcatatcttt actgatgttg ttacactcct atgaagtttt ttatatatta
     9361 taattttttt attcttttat aaagtatccc ctataaagat ggttctatga aaattttctc
     9421 tatattgttt aacgtatata gagattttgt gtataattat aaggtattta atggaaaaat
     9481 ttatcttact tttatatgtt atcaaaatga tttattaaat acaacagaca gcttgataac
     9541 atgtaattat ataaatataa tttatgtaga tagttgtatt tttaaataaa tttttacata
     9601 tcaataatta ttgtctattt tatataagca acaatcaaac taaaataaaa gtttaagaaa
     9661 agagttgatt aaatgcataa acctagttat aaaacagtat ttcattcata cattattttt
     9721 ttcatgattt ttatttgctt aatcctgaca gtaataggta ttttcttatc acttattact
     9781 attcagaaac caaatgggga atttattcgt agtgactggc cgaaaaaatt tacagaaaac
     9841 tttaatgaac aaatcgtatt tatagataat aagccacaag taaaacagtc tggattagaa
     9901 aaacttcgag aaaatcaatt gtggttgcaa attttagatt ctagtggaaa tagagtttat
     9961 gggtttttag aaccaaaaga gcataaagat tattattcta attctgaatt attagcatta
    10021 gctaaccaaa ataattcaag taatggaact gtatacttta atacaattaa aaataataaa
    10081 aatgactata tttatattat atattttcca ttaaatatat ctaaagtgaa tatgtttttt
    10141 gatggaggta aatttatagg tggtaagtca attgtatttt caatcatagg gattatattt
    10201 ttaataattt tggtttctgg aattgcttat ggttactggg taacaaagtg catatcaaat
    10261 attacatctg ctgttagtga tatttcaaaa cgaagctatc ttccagttaa tattaaaggt
    10321 gtttttgctg atatatataa tagtttaaat attttaaatg aagaaattca taaaagtgat
    10381 gagatacaaa ataaaactga tattatgaga gaagagtgga tttcaaatat aactcatgat
    10441 ttaaaaactc cactttcacc aataaaaggg tatgcagagc ttcttacaga tagtagtact
    10501 aattattcac ctgaaaaagt atataaatat gcttcaatca tgttaaagaa catagaatat
    10561 acaaatacac ttataaacga tttaaaatta acctatcaat taaaaaatgg aataatccct
    10621 ttaaataaaa ataattatga tttagtaaga ttcttgaaag aattatccat tgatatttta
    10681 aattatccag aatatgaatc aaatatagtt gaatttaaga gtaatataga taatattaac
    10741 tttgattttg attgtacact attaaaacga gcttttagta atcttattgt caatgctttt
    10801 acatatggag ataaaaatac taaagtttgt gtacatgtaa cattaaatga aagaattgaa
    10861 atcattatat ctgataatgg aaaagggatg tcaagagaag aagcatcaaa tttgtttaat
    10921 cgttattata gaggaagcaa tacaaaacaa aaaatagaag gaactggttt aggactagca
    10981 ataacgaagc aaattattga agttcatgga gggaatatat ctgttgaaag tatacctgga
    11041 ttaggtagct cattttatat cacctttcct atttaattaa ggttaattaa ggttaaataa
    11101 aaaatatatt aaggttgata gattattata tttatatagt agtttatcaa tcttttttat
    11161 ttaaggggtg atttgattaa aaatattata aatagaatga taaaagtatg gaggagtcta
    11221 ttagagattt aatttagatt aaagtataaa aatattagat tgaaatatct tagataaggg
    11281 aagaaggtgt aagtttgaaa attatattaa agtatatttt cacaaatatt aaagaacgaa
    11341 aaattagaac agcagttatg ttattgtcca tagttttatc aacagtttta ctttttgttt
    11401 cattctcaat tggattatct tatgaaagtg cacaaagaaa aatggcaaaa ggtatgtatg
    11461 gtacagcgac tatatctgtc caaagtaaaa atccagatat attaaccaat ttagaagaca
    11521 tacctgattt aaatactata aaatcaaagg ttggtgtatt ggaaagctct gctatttata
    11581 ataaaggggg atactatgaa gaatttagtc ttatatcagc agacttaagt caactaaata
    11641 aaatcaacaa gccacgatta gaaaatggcg atagtattac tgatttttct ggtgataaaa
    11701 ttattcttcc aaatcgcttt acatcaaaat ataaaataaa aaaaggtgat tctattactt
    11761 tgcagattta tggaaagtct tatacttttc aagttagtga tattgcatct tatgatacag
    11821 tatttttgag gaacacaaga ggtgtaaatg cactgctacc caaagagact ctatcaaaaa
    11881 ttataaataa aggcagtgga tatacaagag ttcttataga gtcagaagaa gaaattacag
    11941 aaaatctagt aaataaatta tccgaagaac tttctactga aaaatatatg gtatcaaata
    12001 ctataaatga aactaagata atttcagatg ctagacaaaa gacaatgcct ttctttttaa
    12061 taagtttttt tgcattaacc cttagtatat ttataatata cagcagttat aaggttatta
    12121 cattagaacg tcttcctttt attggaacat ttagaagtat tggtgccaat gaaaaaactg
    12181 taactcgtat attgatgctt gaaagtatat tatacggaag tataggagga ttaattgcta
    12241 ttcctattgg tgtagttgtt ttaaatttaa tgcttcatgg gttaggaaat tctctcgaac
    12301 aaggaatatc aattccagta gtaatatcac ccattggagt gattatatct gttatagtag
    12361 caataatcgt ttcttcattt agtgcatata tacctgttaa aaaagctagc catttgccga
    12421 taaaaaatat tgtactagga acagtagaag aaaagaatgt ttcaaatcgt tctatacttt
    12481 ttataggaag tataatgttt attttatcta ttttacttcc aagaatatcc cctgaaaata
    12541 cattgtatct agctggtgga ttttcattac taggattaat tgtagctact attgtgttga
    12601 ttcctcttat tactgatatc atgtctatag tttttgagtt tgtttataaa aatattttag
    12661 ggaatgaggg taaattagct gctagaaata tgaaaaataa caaaaatata atacagaata
    12721 tcactttact atttataagt atttctgcag ttattgcaat aagtgttgtt ggtaactttg
    12781 tcaaaactta tataacagat gtgtttcgtg acgcagaatt acaaggtttt gcagatggaa
    12841 atatgaatga agagtttatt gaagatgtta gacatatgga aggaattaaa aaaatattac
    12901 ctctttatgt tatgaataat gagatttctg gtaatggtgt aacattatca agactagagg
    12961 gtacagataa tataaaacta tataactcta tgtttggaat caattataca aattttgaga
    13021 taaaaaaaca agtaatagaa gcttttaatg ataaacgttc tgttattttg aatgaagata
    13081 ctctaaaaaa agtaggctta tctataggag atacaataac tctatctaat gataagtatg
    13141 atttttctta taaaatagta ggtagtttta aatcccgtgc aaatgatgta gaagctgtta
    13201 taccatcaca ttatgcagtt aatgattttg ataaaactaa ttatggtttt ttagtatata
    13261 cagcagtcaa tccagatgct gttatgattc agataagata tttatttgga gatacatata
    13321 attggagtcg tacagttgaa gaatttaata atgattcttt aaatactata agtagttttt
    13381 tgtctcctat gaataagatg acctatttca tttttcttct tgctacagta ggtattatta
    13441 ataatctact gattaattat attcaaaaaa gacgttctat agctatgtat aaatctatag
    13501 gactaagtaa taaacaaaat ataaaagtaa cattgattga aggggttact tcaggtttac
    13561 ttggagctgt tattggtatt gtgatatcta tacttgagat tcaaactatc tttatagttg
    13621 caggaccaaa aatttcaatg aaaccagatt tagattttaa aacatttatt attgttgggt
    13681 tattgggaat tatagtaact ttaataggtt cgatagtgcc tattataaaa gggaagaaaa
    13741 tgaaactgat agaagaaatt aagtttgaat aattatggag gttaatatga aaattacaca
    13801 taatagttta gctgtagaag caaaaaatat tatcaaagaa tataaaattg gaaatacaac
    13861 aactcgagtg ttaaaagagg tatctttaca agtaatgaaa ggtgaatttg tttctatcat
    13921 gggtcaatct ggttctggta aaagtactct tttatatatt ttaggtggtt tagatactcc
    13981 aacgagcgga aaagtgtata tgaatggagc tgatatatca cattttaatg atgagaaaat
    14041 gagcataata cgtcgtagaa atataggatt cgtatttcaa ttttataact taataccaaa
    14101 tttaaatgtg gaggaaaata taatgcttcc tttgttatta gatggtaaaa atttaaaaga
    14161 ttataaaaat caattagatg aaatcttaga tatagttggt ttaactgata gacgtaaaca
    14221 tacaccacga gagttatctg gtggtcagca acaacgtgtt gctatagctc gagctttaat
    14281 tggaaaacca gagattctat ttgctgatga gcctactgga aatttagata gtaaaacagg
    14341 gatagaaata atagatttac tgaataaaat aaacagagac aatggacaaa ctataattat
    14401 ggtaactcac tcaccagaag cagccaaaag tagtagtcgt actattacag tttcagatgg
    14461 tttaattgta taaggactaa ttattgtatt tgagataagc atttttaatt atataaatta
    14521 gacatatgta aagtggatat aagttaaata ctataaatgt gctataatat ta
//
LOCUS       DAMJYX010000084        14540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJYX010000084 DAMJYX010000000
VERSION     DAMJYX010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.086"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            120..1748
                     /gene="aspD"
                     /locus_tag="PC479_002704"
     CDS             120..1748
                     /gene="aspD"
                     /locus_tag="PC479_002704"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2805239.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1869..2864
                     /locus_tag="PC479_002705"
     CDS             1869..2864
                     /locus_tag="PC479_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2805240.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3042..3425)
                     /locus_tag="PC479_002706"
                     /pseudo
     CDS             complement(3042..3425)
                     /locus_tag="PC479_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3652..3749
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3890..4448
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3890..3919
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4734..4892)
                     /locus_tag="PC479_002707"
     CDS             complement(4734..4892)
                     /locus_tag="PC479_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805241.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5186..6556)
                     /locus_tag="PC479_002708"
     CDS             complement(5186..6556)
                     /locus_tag="PC479_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2805242.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6772..7889
                     /locus_tag="PC479_002709"
                     /pseudo
     CDS             6772..7889
                     /locus_tag="PC479_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861579.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8252..8332)
                     /locus_tag="PC479_002710"
                     /pseudo
     CDS             complement(8252..8332)
                     /locus_tag="PC479_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8372..9040)
                     /locus_tag="PC479_002711"
     CDS             complement(8372..9040)
                     /locus_tag="PC479_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2805243.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9893..12313)
                     /locus_tag="PC479_002712"
     CDS             complement(9893..12313)
                     /locus_tag="PC479_002712"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2805244.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12667..13017)
                     /gene="rsfS"
                     /locus_tag="PC479_002713"
     CDS             complement(12667..13017)
                     /gene="rsfS"
                     /locus_tag="PC479_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2805245.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13134..13706)
                     /gene="yqeK"
                     /locus_tag="PC479_002714"
     CDS             complement(13134..13706)
                     /gene="yqeK"
                     /locus_tag="PC479_002714"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2805246.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13707..14396)
                     /locus_tag="PC479_002715"
     CDS             complement(13707..14396)
                     /locus_tag="PC479_002715"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2805247.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4902 a         2378 c         1839 g         5421 t
ORIGIN      
        1 attttaattt catactttac tttttctata aacctattat aattaaagta taatcatttt
       61 tgcaacttgt ttattttatt ataaaaatgt gaaacttatt acataaggag gaatttaaca
      121 tgaaaaatca tacttataaa gaaatcaaaa acatatatgg aaaaataagc ccttttgaat
      181 ttaaggacaa attaatagat attgcaaaat acactgcaaa agaaaataac agagaattac
      241 ttgatgcagg tcgtggaaat ccaaactgga cttgttcaac tgctagagaa gcctttttta
      301 cttttggtca tttcgctatt actgaaactc gttctaattg ggatttaggt catttggctg
      361 gaatgcctca aaaaaagggt attaaagaaa gattttttaa atttataaat gaaaatatag
      421 atatgcctgg agcatattta gctagagata ttataaactt tggtataaat gaacttggat
      481 ttgatggaga tgaatttgtt catgaactag ctgatggaat tattggtgat aactatccac
      541 ttccagatag aatgttacct catatggaaa aaatagtaca cgattaccta gttcaagaaa
      601 tgaaatatga tatatcaggt aaatatggtg atgtagaaat ttttgctgtt gaaggtgcta
      661 cagctgctat gtgctatata tttgattcat taatggcaaa tgaacttcta aaaaaaggag
      721 ataccatagc cttaatgact ccaatattta caccttatct tgaaatacct aaccttcctc
      781 gttacgactt taaagtggta aatataaatg ctaatgaagt tgatgaaaaa ggtgctcata
      841 catggcaata tacaaaaaaa gaattagaga aacttcgtga caaatctatt aaagctttat
      901 tcgttgttaa tcctaataat cctgcttcta tagctatgga tgaaacatct tgtaataatc
      961 taatagatgt aattgaaaat tataataaag atttaatgat aatatcagat gatgtatatg
     1021 gaacttttgt agaagatttt tcatccttaa tgtcaaaact accttacaac actgttggag
     1081 tatattctta ttcaaaatat tttggagtta caggatggag acttggtaca tttgcccttc
     1141 ataagaaaaa tgtttttgat aagaaaataa atgatttaac tggagaatta aaaaaatcag
     1201 tagataagcg ttacagtgat atgagtctta acccttctag cctttcattt atggaaagag
     1261 ttgttgctga tagcagactt gttgctctca accatactgc tggtctttct actccacagc
     1321 aagtacaaat ggcgtttttc tctgcttttg cattgattga caaagtagat gcctacaaaa
     1381 aactcaatat gagtatctgc catagacgtc aaaaactatt atttgaagct ttagagcttc
     1441 caattaatga aaacaaaaac aatgctgcat attacactca atttgatatt gaagaatggg
     1501 ctaaattaaa ctacggagaa gacttattta aatttataag taaaactgca tctccagtag
     1561 atgttcttta taaattagct aatgactatt cagtagtttt acttagtgga aatggattct
     1621 atggtcctga atggtcaatt agaatatctc tagcaaatct atatgatgaa gcttatacta
     1681 agataggaaa agccataaga gagatattaa atggttatat cgttgaatgg caaaaaatta
     1741 agaaataatt atattacact gaaatatcta aattatatga ataaaagagt ttatgtagac
     1801 cttaactact ttttttgata taatcctatc ggaattataa attttattta tgaaaggcag
     1861 agaatagtat gagtattaag aaaaaagttg ccatagtttt tactggtgga actatatcta
     1921 tgacagtaga tgataaagtc ggagcagcta taccaacttt atcaggtgaa caaatactat
     1981 caatggttac caacattgat aaagttgcag atgttgaagt ttttaatttt gatgaaattc
     2041 caggacctca cataacacct tatagaatga tggaattgaa aaattatgta aatgatttat
     2101 tagcaagaaa agatattaca ggtgttgtaa taacacatgg tacagatagt ttagaggaaa
     2161 cagcttattt tctagacctt acaattgaca acatcaaacc tgtaatagtc acaggtgcta
     2221 tgagaagtag ttctgaactt ggatatgatg gttcaagtaa tttatctgca tcagtttgta
     2281 ctgcagtatc caaagatgct atgggaaaag gagtccttgt agttttaaac aatgaagtat
     2341 tattagcttc tgaagctact aaaacaaata ctctttctct aaatacattt aaatctctaa
     2401 ctagtggccc attaggaata attgactgca acgaacttgt ccttacaaga gatattgtaa
     2461 atagaacaat tatagatact gataaggtag aatctaaagt tgccttattt aaagcatatg
     2521 taggagaaaa tgctgacttt ataagatttg ctgttgatag tggttataaa ggtatagtta
     2581 ttgaggcaat gggtagaggt aatattcctc ctcaaatgct ttctggagtt gaatatgcta
     2641 gagaaaaagg acttcctgta gttatagttt ctagatgtca ttcaggtaga gtattcgata
     2701 gttatggtta ctttggttct ggtagagatt taaaaaatct tggttgtata tttggtgggg
     2761 acttaccagg tcaaaaagct agaataaaac ttatcttagc acttggaaaa actgataacc
     2821 ttgatgaaat caaagatttc tttgaaaaag gaatctattg ttaattctaa taacttctat
     2881 ttaattattt attgatattt caacattttt aaatcaaaat ctaatactta tgtcagttaa
     2941 atgtcagcaa aatattttat ccacaagttt tccacaactt atccccaata ttatccacaa
     3001 gaaaaaaggt agcacttata ttagtagcta ccttttattt attaatcttc atctttccag
     3061 ctttttatcc aatttcctat ttctattatt ttttcttcag ttatttcttc tccatcatgc
     3121 aattttgaaa ttaattctga taatggattg ttatgtagac ctccaaaaat tctttttccc
     3181 tctattttga gatattcttt ctcttttatt aatactctat agtgtacatg tttcccaatt
     3241 ctctgcgata tgaaaaaacc ttttttagtt aatctagcta aaagtgtaat tgtagttgta
     3301 tgcttccaag catatgtttt ttctatttca tctgcaacat cttttgatat tacagtttta
     3361 taaccagtat tctatatgta tttcataatt tttaattctg catctggtaa ttttttattc
     3421 aacataatat attccttctt tcaatttatg tcacttcctc aaacttatct agcttaaatt
     3481 aataatagga gctttttatc acaaaaacta caggtaaatt tttctatata ttctacgtat
     3541 ttagtctgtt ttttaagtgt gaaattacgt tcatacctat aaatttattc aattttcaaa
     3601 caatcttatt gaataataac taattctatt gtattattta gttatagaat aaaactaaat
     3661 cggcaaaact agagaaatct agtgacgcaa agctatagag actaagactt atataaatat
     3721 cttatgagtt atgtcagcca gttgccaaag agatattgtt ctttttgttt ttatgaaagt
     3781 tttttagggg gataataatt tatatattta gagataaaat agataaatgt acacatatat
     3841 tgactgctta tattagcagt tcgtatgatt attgtaattt tttggataca atttacattc
     3901 catatagtta atataaaaca agtgtttcga atttcttttc tgaaactata ccaacaattt
     3961 acattccata tagttaatat aaaacgttat atactgaaag cggtgaaact ggttacgtag
     4021 caatttacat tccatatagt taatataaaa cctgtgcgtg taggagatgg taacacaact
     4081 gttgaagaat ttacattcca tatagttaat ataaaacaca catctacatg tttttctatc
     4141 tgagttcatc tctaatttac attccatata gttaatataa aactttttaa tccttcttct
     4201 gaactcccaa agtttatatc atttacattc catatagtta atataaaacc cacgaaattt
     4261 aaggtcgata tatcagattt gaaaaaattt acattccata tagttaatat aaaacaagct
     4321 aaatggagca accgaccaag tcgctagaaa taatttacat tccatatagt taatataaaa
     4381 cttttctatt aagtgtaagc agaatgcatg gaatatgaaa tttacattcc atatagttaa
     4441 tataaaaccc caaaataaat tgagtatttc caatgcctac acatatacaa atctctcaaa
     4501 tttgcagtga accatgagta gtgcaattga taacacttat cacaagtcct caatgcctta
     4561 tatttcaact gttaagcctt aatttcttca aaaaatcgaa cactgcaaaa tttctatgac
     4621 tttattatat cataaaaata ttatttttga atatctgtac caatttgtga tataataaaa
     4681 gcaagaagaa ctacaatcta ttttgctgta gagtggagtt cataatttag aaattactta
     4741 gttttatgga atctgatttt taaatcaaat tcccaaccac tcttagttgc cgctttgagt
     4801 ggttttttac gctttctaaa tagtttgcta gctatataaa ctattaagct agcagataga
     4861 cttgctagta tgccttgtaa aaaattatcc atgtatattc acctcccttc ttaacgtcgg
     4921 gaggataatt ttttgataca tgaactccac tctataaatt gtagattaca tcttcttgct
     4981 aaaaatatta taacatataa ttcttacata ttttacctat ttattacttt ttacattata
     5041 ataatattct tttaatcaaa aaaacaccta ctattaaagt aaatgttttt aagtattttt
     5101 aattttaaaa tccatatagt tcatatgaaa ctaaatataa taactcaaca tatgagcaat
     5161 atcgagacat ttacataccc actagttatt tatattcaaa aaaatcttct ccatccgggt
     5221 tataaaataa tattctactt gccaatcctt gttcatattt ataattttct ttatcaccta
     5281 cataagaaaa tgttaatgtc atgcttcttg atggatctaa tgaaattata tctaaataaa
     5341 tagtacattt ttctcctggc tttaatacac cgaaactatt aaaatccatt tttattttag
     5401 cgtattgccc catatataat gttgaattag cttctccata aacttttaat tttgttacat
     5461 ctaaattttg tgtacttgta ttttctatat ttataggaac tgttgggtct ttatattttt
     5521 cagtataata ttcaaggtta tcatcataac attcaagata acttccttta tcttgccaaa
     5581 ttatgtgatt tgaatcaaaa tcaatcttta atccacatat tatattttta tttgaagatg
     5641 gaagactaat attattgatg gtattttcac actctagcat aattttatca cttagatttc
     5701 ctatcccggt tacttttgca gcatttttaa gtatatcttt attacttcca atatcaacta
     5761 gtacaatagg attattttta gccattggtg aagcaactaa agcataaact aaatcagtac
     5821 cactagctat ataaaattct tttgcgccat tatagaactt atttactatt tttttattag
     5881 tatcatatct atctactcca cccaatcgag ttgaattagt atcatttact aaactatcac
     5941 tcattgatga agtgccacct attgcataac tttctatacc agttgtatta aatggaacac
     6001 tttttccatc agttaatact atagctgctt tatctctaac tgctacaggt gcagcactca
     6061 tagcatctgg ctcacctttg aaagcgttag ttaatattac tttatttact ttattaatgg
     6121 aatttatttc ttttgctaca ttataacttg tcttaatcct atcattacct tgaagccttc
     6181 tagtctctat tcctttatct tttagaaaag tttctgttgc tttatcaatg gaactttctc
     6241 ctcctattat gtataccttt ttagcttttt ctactctttt aagagttgca ttaggtatat
     6301 tattcttttt agtcaaaagg attggtgcat ttgttgctcc tgcaagtcca ctcgcactta
     6361 gcccatcagc cattgtacta tctgcattta taagtattgc agtagtataa ctttgtttgt
     6421 cagctatgat accagcagtt tcgtatttat ctgctccttg aattttatca attttatcaa
     6481 gtgcattagc tgatatagga aaaccagcaa caaacattga cactaaccct agtgataata
     6541 atttctttaa tttcacaata tctccccctt ttatatatga aatactatat atcttaataa
     6601 ttctacatta aattttcttt tcctccttta acgatattta tcttcctgtt aatttaagtg
     6661 tatacaaata cgtgttataa tgtaactgta aagaattaaa aagataatga cataattaga
     6721 gtagtacaac tgttctttta taaaactaac ttataaaggg agcgtacata tatgaatatc
     6781 aaatcagctt ttataagaaa aagaggggaa aaattctatg tatatgtgga gtacatagag
     6841 gaaacaactg gcaagataaa acagaaaagt tatggaagtt atgaaaagaa aaaggatgct
     6901 gaaaaacatt taattgaaat aaaatctact ataaacaata ataagtttgt tgctccaaaa
     6961 gatatgacat ttgtagaaag atgttataaa tacatggaca tgaataaaaa taattggtct
     7021 ccatatactg ttacaaatac aaaatcttgg gttaaaaatt acatagaacc tctttttaag
     7081 aatctaaaac tcacagaagt aaatcctagt tcagtacaag cttttgtaaa ttataacttt
     7141 aataattcta cctttactag tgctaaagta aggtatagct ttctaagttc tgtaataagg
     7201 gaagcatata gattaagaga aatacaagaa aattcatgtg attttataaa acttccttct
     7261 aaagaaaaat attctacaaa tgaaatatac aatagaaaag aagtattatt gctaatagaa
     7321 aagttactta gtaataatat agaaatacct attcttttaa tgttacttct aggtttaaga
     7381 atgggagaag ctactgggct taggtggtca gatgttgact tagataacaa tctgataaat
     7441 gttaatcaaa ttcttgtcta ttctaatggc aaaatatctt ttaaagagcc aaaaacacca
     7501 aaatcaaaaa gagctttatc agctccaaaa gaattaattg aaaaattgaa aattaaaaaa
     7561 ctaagacaaa gcaaattaaa attgtaaggt atcctggaaa acagaaataa tttagtttgt
     7621 ctaaacacca atttaaatcc gtttctacct tcttcactaa gaaaaacgtt tcacaatttt
     7681 acaaaaaaaa taatttaaaa aagattagaa tacatgattt aagatataca agtgccactc
     7741 tgctgctttt agggggaact aatatgaaag ttgtttctga aagattgggt catacagata
     7801 taaaaataac tatgaataga tattctcatg tacttaaaga aatggacaaa gaagcttccg
     7861 acaatctaag cgaacttcta tttaaatagt aaaaactgac tgtcagttaa atgtcagtta
     7921 aacctaaaaa aataggtttt gtcagttgat tatcagtcaa ttattttata atagtttcac
     7981 ttaaaaacgt ctatatctac acattttact atttgtttat gcttattaat atctatctgt
     8041 tattattaat tctaatagct tctatttaat tatttagttc agtttacagg aaactacaca
     8101 caaaccataa aataggatgt tgacaatata acagatagta tgctcctgta aaaggtaacg
     8161 tactatctgt tatgttgtct ggatttccaa atggttaccc tttggcacac aactttgcta
     8221 tctccttggt aaactaaaag tttgtaatct aaaatcctaa tccatctcta taaaatttaa
     8281 ttgacttctc catacttttt acacctaaac atataaaagt aattttattc acttaatgac
     8341 ctccataatt cccaaattag ctatttggaa cttaactaaa aaatcctaat ataagggcaa
     8401 ctattataat ggcaatcgga atacatactg tgtaatcctg ttccatagag tcatcatctg
     8461 aatgtagttt tacaattcgc aatttagcaa tcagtttgaa gcagaaatag cctattattg
     8521 ttccaattac attcgtgagt aagtcgtcta tatcggttgc tctgtgtaac gtaaaaagct
     8581 gaatagtttc gataaaaaga gataatccaa aaccaatcaa gaatgtattt ctcaaacgtt
     8641 caaaggtttt gctgataagc ggacataaaa aacctaatgg gacaaaaagt aaaatattca
     8701 aaataaaact caaactaaat ccatcactaa aaggaataag atttatgttt ggattaaaga
     8761 acgtttcccc taactgtgac agtcttatat attcactgaa tgtagggata ccaacaatat
     8821 gcgatagtgt taaacataag tagtaataca ttgccagtga taaaatagat gtttttacct
     8881 ttggaatatc actatttttc ttgaatatta caactaagaa aataccaatc aagataattc
     8941 ccaacaagaa aataggatta attaacaata aatctttgat agcttcaaac agactgagtt
     9001 gaatttctac acctgattga ttatttgtta cagtctccat ttgtacatac ctcctcataa
     9061 catttcaatt atttctaaaa cacaaaatag aagataaatc ttaaaatcat cctatcaaaa
     9121 tttttaaatt tgcctttatc agtttatacc accagttaat tcctgtctta cttctgtatg
     9181 gtattcttgg gattctttca tcaaattaga caaaaaatgg ttaccatatc aataaactgg
     9241 ttacgctcca tatctcccga agcaaccatc ccctgagaat aaatccgtat caggaatctc
     9301 cacatctact gcacctatca caagttattt gataacatcc tcagacaatc ccaacttgga
     9361 tatatcgaca acacccatta gaataccact gctgaccata cttgttttcc tgctgataat
     9421 tttaatcaac tgggaagttg aaacgctatc ttttttacaa gttccttttt agaaacatgg
     9481 tagctattat atgaatctga atttcgttgt ctgggtgttt catataaagc atagatacgc
     9541 accttttttg cctgttttca ttatatagaa aaaaatttca aaatacaata attatatttt
     9601 tttcgtttaa gcttagagca gttccttgat gaacaacact ttggaaactg aatgatttac
     9661 ataccactta atacattaac tcgatttaac attaattata ataatattta aaacaacaac
     9721 tagggtatat aagaatgaac tttttagttc tttaatcatt tttttgtata aaatcaaatc
     9781 tatttttata atttcaacaa ttttataatt tcaacaatga aaaaaggctt atctctattt
     9841 tgagacagcc tcgtttattt tataaattta tatttttgtt catatttttg agttatttaa
     9901 caactatatt tactagtttt ttaggtacag ctacaacttt aactatagtt ttaccttcaa
     9961 ctaatccttt tattttttca tcttctaaag ctaccttttc catctcatct ttagatatat
    10021 ttgagttaac agttaatttt cctctaactt ttccattaac ttgtacaaca acttctattt
    10081 catcttttac taaagctttt tcgtcatatt taggccagtc tatgtcaaat acacttcctt
    10141 ctttacctat catagtccat agttcttcac ctaaatgagg ggcaaatggt gcaattatag
    10201 ttactatagt ttgtacacct tcttttataa ctgcttcgtt tatattatct aactctttat
    10261 atttatacat atcatttatt aattccataa gagctgatat agcagtatta aatccgaatt
    10321 tttcacttaa atcagcagta acttttttaa gtgtagagtg tatagtatat ctcatatcct
    10381 tatcttggga atttaattct ccaaactcta catccttttt aaccacatct gctaactcgt
    10441 caaccaatct ataaacccta tttaagaatc taaagcatcc atcaacacct tgctctgacc
    10501 agtccaaatc tctttctggt ggtgctgcaa ataatacgaa aagtcttgca gtatctgctc
    10561 catactcatc tattatatcc ataggagata ctgtattccc tttagactta ctcattttag
    10621 aaccatccat aagtaccatt ccttgagtaa gtaagttttt aaatggctca ttgaagtcta
    10681 ccattcccat actcttaaat gcctttacaa accatcttgc ataaagtaaa tgcattatag
    10741 catgttctac tccacctata tattggtcta ctggatgcca tctattaact aattccttac
    10801 taaatggttc attttcatta ttgctatcca catatcttaa gaaataccaa gaagaatcaa
    10861 cgaatgtatc catagtatca acttcccttc tagctggttt tccacagtga ggacaagttg
    10921 ttgacataaa ttgctttgaa gtcgtaagtg gagattcccc ttttccagta aattcaacat
    10981 ctgttggtaa aagaactggt aaatcttcct ttttaactgg aactattcca cattcatcac
    11041 aataaaccac aggtatagga caaccccagt atctttgtct tgaaactaac cagtctctaa
    11101 gtctataatt aattgttctc tttcctctac cttctttttc taacttatcg attattcctt
    11161 caaatccttc tgaagcatct aatccattaa attcttctga atttatcatt ttgttatctt
    11221 ctgttattac atcaattatg tctaaattat atttttcagc aaaatctcta tctctttcat
    11281 catgtgctgg aactgccatt atagcacctg ttccatactc aactaataca tagttagcta
    11341 tccatagagg aactttcttg ccatttaaag gatttataac atatctacct ataaacattc
    11401 cttctttttc tacatcagct gctgttcttt ctatttctgt catagtatgc attttttgaa
    11461 caaaagcttc aacatctgct tcatattcag tccctgcaac caattcttta actaattcat
    11521 gctctggagc aagtaccata taagtaactc cataagttgt gtctggtcta gttgtaaata
    11581 ctgtcataga tttatcagtt ccatctatat caaaaactaa atctgctcct gtacttttac
    11641 ctatccaatt tttttgcatt gttttaactt tttctggcca tccatctaat atatctaagt
    11701 cattaagtaa ttcttctgca aattcagtag tcttaaaata ccattgttct aaatctttct
    11761 ttaatactgt tgctttacat ctctcacatg ctccttgaac aacttgctca tttgcaagaa
    11821 cagtctcaca agaaggacac cagttaacat atgatttttt cttatatgca agcccatgct
    11881 ctaaaaactt caaaaatatt tcttgtgtaa atctatagta ttctggcgtt gaagttgcaa
    11941 cttctttatc ccaatcataa ctaataccta ataaatttaa ttgctctttc atttcttcta
    12001 tattttccat tgtccattta tgtggatgta ttccatgttt tatagctgca ttttcagctg
    12061 gtaatccaaa agaatcccat cccattggat gaagtacatt atatccttcc atttttttga
    12121 atcttgcgac aacatcacct atagaataat ttctaacatg tcccatgtgt atttttccag
    12181 atggataagg gaacatttct aatgtgtaat aattaggttt ttcagtttgt gcagtatcca
    12241 ttttatactg attattatct ttccagattt tttgccattt cgattctact tctttaaaat
    12301 tatatacgct catattttgc ctcctatttt taattgtgta taactacctt atttagtatt
    12361 atttacttat aatttccaaa taattctttg aaaataaaaa atcctcgtca ctaaacttac
    12421 gtttagggac gaggataaat cttcgcggta ccaccctaat tagcctacta catatataga
    12481 ctctcttaga tagcttttaa tccagccatg gagaatagat tttaatctag taagttcaaa
    12541 gttatataat attagttctc accttccact aactctctga aaaaatataa tcttttactg
    12601 ctctagtcta ttcaatttaa tataattttt gaaattttct aattataata acatatatta
    12661 tcatgtttat gctaatgtgt caatatctat ataaggtgca tctttccata atttttctaa
    12721 attataaaac cctctatttt cctcattaaa tacatgaacc attatgtctc cataatcaag
    12781 gagtacccaa gtttgagttc cttgtccttc tttacctctt ggctctaatc ctagttttgc
    12841 taattcatct tccacattgt cagcaatagc ttttacctgt ctctgagaag atgctgtagt
    12901 aataataaaa taatcacaca aactagaaac ttttcctata tttattataa ctgtatcttg
    12961 acccagttta tcttctatgg catcatatgc tatttttgtc atttgttcca ctgtcatata
    13021 aaaattcctc actttcctaa ttaaaataca cttagttatt gtaacatatt aatacgattt
    13081 tgtaacatag tcaaatttaa atttattaat atgtttgcct ttatatagct tttttataat
    13141 aacttttctt taattagata gtttcttgcc ataactgttc ttggatgtat ctcttctttt
    13201 ttattgatga caaacattaa agtattatta aaagaagtta aaagtgcttc atcaagcttc
    13261 cctccatatg ctaattctct taatgtatct acaactggga attttcttcc ttcttcaatt
    13321 aaatcagcta tgtaaattat tttttctaaa agagacatgt cctctctccc tgttgtatga
    13381 tatttgattg catttataat atcttcatct tccacttcaa attcatactc gcatatgtac
    13441 gccccaatta cactatgaga caaggaacga tttccatcct ctattgggtc taaatatatt
    13501 tcatacttat ttacataatc ctctatttcc ttgtcactta aatactttgc acaatcatga
    13561 atcattcctg ctaagtaagc tttttctttg tcacatccat aaatctcaca taatttttca
    13621 gcacattttg caacattttt tgaatggcta agcctcccca ctggtagcat ttgatttagt
    13681 ctttggttaa tattttctaa gttcatttaa tcatctccat attgatatag tttattttca
    13741 tataaataat tttctacatt ttcgggtact aagtaacgta ttgacttgcc ttcatttagc
    13801 tgttccctta tatatgtgga cgatatatcc agtgatggaa cataaactgt tataatgtca
    13861 gcattatact tcttagttag tttttctata gtctcttgtg accttaacaa actaattcct
    13921 ggtctagttg cggctataaa agtagctaac tcaaagtttt tctttacatc tttccactct
    13981 tcaacactac aaattgcatc tgcacctgtt ataaaataaa tgtctgcatt tttgtatttt
    14041 ttctttaagt attttaatgt atctattgta taagtccttt tctttctttc gatttctact
    14101 tttgaaacta aaaaatcttc gttgctttca gttgctagaa gtgtcatatt atatctatca
    14161 tacttatctg tagtgataca taatttatgt ggtggatttc ctgatggtat aaagattatt
    14221 ttgtcaatgt catacttgtc tctaataaat tctgctgttg ctaaatgagc atagtgtata
    14281 gggtcaaaag tacctcccaa tatacctatt ttgattttgc ctttatggcg attaaatttc
    14341 tctagttttt cagtattttt caactctgcc atcttaacta gattttcact tctcatattt
    14401 tgtctccttt taatgttata atactattgt accattattt tttatttctt aaagcttata
    14461 atttgttttt ttgggtatat aattatattt atatgctaaa aaacacttta aaaaataatt
    14521 atttaacata attctgcttt
//
LOCUS       DAMJYX010000085        14498 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJYX010000085 DAMJYX010000000
VERSION     DAMJYX010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.087"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     misc_binding    51..289
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            357..2996
                     /gene="alaS"
                     /locus_tag="PC479_002716"
     CDS             357..2996
                     /gene="alaS"
                     /locus_tag="PC479_002716"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2805248.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            3021..3278
                     /locus_tag="PC479_002717"
     CDS             3021..3278
                     /locus_tag="PC479_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2805249.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            3288..5459
                     /locus_tag="PC479_002718"
     CDS             3288..5459
                     /locus_tag="PC479_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805250.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            5665..6090
                     /gene="ruvX"
                     /locus_tag="PC479_002719"
     CDS             5665..6090
                     /gene="ruvX"
                     /locus_tag="PC479_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2805251.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            6168..6455
                     /locus_tag="PC479_002720"
     CDS             6168..6455
                     /locus_tag="PC479_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2805252.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            6737..7195
                     /locus_tag="PC479_002721"
     CDS             6737..7195
                     /locus_tag="PC479_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2805253.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(7312..8049)
                     /locus_tag="PC479_002722"
     CDS             complement(7312..8049)
                     /locus_tag="PC479_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2805254.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            8266..9942
                     /locus_tag="PC479_002723"
     CDS             8266..9942
                     /locus_tag="PC479_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2805255.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            10148..10348
                     /locus_tag="PC479_002724"
     CDS             10148..10348
                     /locus_tag="PC479_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2805256.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            10402..11565
                     /locus_tag="PC479_002725"
     CDS             10402..11565
                     /locus_tag="PC479_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2805257.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(11776..12198)
                     /locus_tag="PC479_002726"
     CDS             complement(11776..12198)
                     /locus_tag="PC479_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2805258.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(12219..13055)
                     /locus_tag="PC479_002727"
     CDS             complement(12219..13055)
                     /locus_tag="PC479_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2805259.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            13353..13949
                     /locus_tag="PC479_002728"
     CDS             13353..13949
                     /locus_tag="PC479_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2805260.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            13979..>14498
                     /locus_tag="PC479_002729"
     CDS             13979..>14498
                     /locus_tag="PC479_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2805261.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISV"
BASE COUNT         5646 a         1563 c         2595 g         4694 t
ORIGIN      
        1 ttgattaatt ttgtaaaatt tagtaatata attagtatat taattaaaaa acaaatacag
       61 tgataaggaa tagtaaaata tttgatgtct tacagagaga gggaaatgct ggaagccctt
      121 agaatatgat atttgaagca gccttttagt agcaatcttg aacaatttat cttttataaa
      181 gaagatttaa gtagagattg acggtagtta cgttatagct aaggttttcc gaagatatca
      241 tactttgtat gataattagg gtggtaccgc gattgtcctc gcccctataa atttatttat
      301 agggacgagg ttttttattt aaacgaaaaa ataaaattta ttaggaggaa catgatatgg
      361 aaaagatggg attaaatgaa ataagaagta agtttttaga attttttgag tcaaaaggtc
      421 attatgtagc aaatagttat tctttagtac caaacaatga taagagtttg ttacttataa
      481 actcaggtat ggcacctttg aaaaactact tttcaggtgt agaagtacct ccaagtgtga
      541 gaatgtgtac atctcaaaaa tgtataagaa caggagatat agaaaatgta ggtataactg
      601 caagacatgc tacattcttt gaaatgatgg gtaatttctc atttggagat tactttaaaa
      661 gagagtcaat aaaatgggga tgggaatttg ttacagaatg gctaaatata ccagaagata
      721 agatatgggt gactgtatat gaagaagatg atgattctta tgatatatgg gcaaaagaaa
      781 tgaatttccc agaagaaaga atggttagac ttggaaaaga tgataatttc tgggaaatag
      841 gtacaggtcc ttgtggtcct tgttcagaaa tatattttga tagaggagaa gaatatggat
      901 gtgacaatcc agactgtaaa ccaggctgtg attgtgatag gtatttagaa ttttggaacc
      961 atgtatttac tcaatttgat agagatgaag aaggaaatta tagtctatta gaaaacaaga
     1021 atatagatac aggtatgggt cttgaaagaa tgggatgtat tatgcaaggc gtagacacta
     1081 tctttgaagt tgatacaatt aaatctatac tagaagcagt tgagaagtta acaggagtaa
     1141 aatatggaga gaatccaaaa aatgatatat caataagaat aataactgac catataagag
     1201 ctgtaacatt cttagttagt gatggagtgc ttccatcaaa tgaaggaaga ggatatgttc
     1261 ttagaagact ccttagacgt gctgctcgtc atggtaagtt attaggagta aaagaattat
     1321 ttttacagaa actaatagat gaagttataa aagtaaatga taaagcatat ccagttttag
     1381 ttgaaaaaga aagttatatt aaaaaagtag ttggaataga agaagaaaaa ttcaatgaaa
     1441 ctatagacca aggaacagaa atattgaact cttatataga agtattaaaa aatgaaggaa
     1501 agactgtcct aagtggtcaa gaagctttta aactatatga tacttatggc ttccctatag
     1561 acttaactaa agaaatatta gaagaagaac atctatctgt agatgaagaa gcttttaatg
     1621 aagaaatgga aaagcaaaaa gaaagagcta gaaatgctag aggaaatatg gatggagaaa
     1681 gttggaagga agaccctcta tcaaaattag aatctacagt tgacagtaca tttaatgggt
     1741 atagtgaaat ttatggtgaa ggaactattg aagctatagt taaagatgat gagctagttc
     1801 aaagtgcaga agaaggcgat aaggtatcta tagtactaga taacactact ttctatcctg
     1861 aaggtggagg acaagttgga gattgtggat tgataactaa tgaaaactta gttttagaag
     1921 tattaaatac taaaaaaggt gctaataata gtataaaaca tataggtata ataaaatcag
     1981 gaagaataag taatggggac aaagtgaaga cactcgtaga tagagaaact agaatgtcag
     2041 ctgcaagaaa tcacagtgct acacatttac ttcataaggc attaagagaa gtattaggag
     2101 aacatgttaa tcaagctggt tctttagtaa ctccagaaag acttagattt gatattactc
     2161 actttgaagc gatatcaaat gaagaattaa aagtaataga agaaaaagta aataatgtta
     2221 ttttatcatc tttagacata aaatgtgata ttatgaatat aaaggaagct aaagaaaaag
     2281 gagctacagc actatttggt gaaaaatatg gtgatgaggt aagagtagtt tctatgggag
     2341 attattctac agaactttgt ggaggaactc acttgacaaa tacttctcaa gtaggaatgt
     2401 tcaaaatatt atctgaaggt ggagtagcag caggtgtaag aagaatagaa gcaataacag
     2461 gaaaagctgt ttatgaatac ctaaaagaaa gagatggaat tatctctgaa gtttgtgtaa
     2521 acttaaaatc taaagaagat aatttaattc aaagaataag ttctttatta gaagaaaata
     2581 aaaacttatc taaagaattg catgatatga aagctaagat gagtttacaa tcagttgatt
     2641 ctatttttga ttctaaagtt gaagtaaatg gagttaattt gataactaat aaatttgaag
     2701 gtatggatat ggatacacta agagaaacag cagataatct aagagataag cttggttctg
     2761 gtgtagtagt acttgcaaat gtagtagatg ataaagttaa ctttgtagtt actgccacta
     2821 aagatgtatt agataaggga atacattcag gtaatattgt tagagaagtt gcaaaaatag
     2881 ctggaggaaa aggtggagga agacctaata tggcacaagc aggagctagt gatgtatcta
     2941 aggttgacca agctttaagc tatgcaagtg aggttattaa gacacaagtt aaataacatt
     3001 cactcaagga ggtttttagt ttggaaaaag atttagacta cactatgaaa tttgaaggaa
     3061 taccagaaga taggatgagt gtaggagata ctattgattt tgtatataaa gcattagttg
     3121 aaaaaggata taaccctata aatcaaatta tagggtatct cctttctggt gattctagct
     3181 atataactag tcataaaaat gcaagagcta taattaaaaa atttgaaaga gatgagatac
     3241 ttgaagaagt aatcacgcac tatctgaata gaaagtaggt caaataaatg agaaaaaaga
     3301 ttatgtcgtt gtttgcaata gtcattatga tttttgtatc aactgctagc tatggttttg
     3361 ctgaagaaaa aggcaaggtc atatttattg atatgaatag gaccaatctt ggaagtatga
     3421 tgaatattcc aatcttgaaa aatgagatag aaaagagagg ctatgtagct ctcatgaata
     3481 taagagggga tcaaggtact gatgatagaa ggtcttatgc aagtatgggt gcaggaacaa
     3541 gagctagtgt aactactgaa gactatatca attttcaaaa agtagaccaa gatactgcta
     3601 aagcttttga agtcgcaaca ggtcaaaaag ctaaagaaat aaatgattta ggcattaata
     3661 gatctataaa tgaaaatctt gagtatggag aatatggagc aaatttaggg tcattaggaa
     3721 aaactttatc agaaaatggt ttaaagacat ctgtaattgg aaatgctgat ataattgaaa
     3781 ataatcaatt agttaaaaac agaaacttat gtttagtagc tatggaccaa tatggaagga
     3841 ttgattcagg taatattgat aatattaata agaaagattc taaaatgcca tttggaatat
     3901 ctacagatta tgataaatta ctagatgaaa ctaaaaaatc ctataaaaat agtgatgcta
     3961 tatttgtaga acttggagat acatatagat tagatatgta tagattaaat ctaaatgata
     4021 atacttatga tataatgaag aaaaatattg taaaaaatat aaataaatat cttgaacaag
     4081 tattttctat ggttggagaa aatgacactg tttatatagc aagtgcattt ccaagtgacc
     4141 tagattataa gaataagaga agactttctc caataataaa atttagtgga gaagggaaag
     4201 gtctactgga atctgccaca acaagaaagg ctggaattgt tgccaattta gatgttggag
     4261 tagatatact aggtacattt ggtcttgaaa ataaagagat ggtaggtaga agttatactt
     4321 tagtaaataa agaggacaat attagctatc taaaatcact ttatgaaagg atagtgtcta
     4381 tatcttcaat aaggtcaact attataaatg gctttgtagg cgtaatatct gcttcttggg
     4441 taattgccat gtttgcgatt ttctttagaa attatatgcc acataaagag cgtgtattca
     4501 aagtacttaa ggagtttata aaattaggca taataatgcc actatcattt atgttagctc
     4561 ctttgatgaa ttttaaaact ccaatttcaa tatctttggg aatatttgca actacgctaa
     4621 tattatactt gttaggtaga aaattattta aggacaatga tataaagcac atgggatttt
     4681 ttgcaacact tactatttta gtcatagtta tagattctgt atttggaaca tacttaatgc
     4741 aaaataacat aatgagttat gatgctgtca taggagctag gtactatggt gtaggaaatg
     4801 aatatgaagg tgtaactata gcaagtgcag tatttggctt agcagtatta ttacattata
     4861 agaaggtatc taaattactt gttgtaattt tttcattaat aatacttata actagtgcat
     4921 atccttcaat gggtgcaaat gttggtggag ctatatcaga atgtgtagca tatttgttgt
     4981 ttataatgct tatatatgac attaaaatag attttaagaa agctgtatta ttagctgttt
     5041 cagctgtagt tgtcgttttt gcatttgcag ctttagatat actttcaggt agtgaatctc
     5101 atttaggtat gtttgtacag agaattttcc taaatggacc aggagagata ttacaaacat
     5161 ttggaagaaa aattcaaatg aatatgcaac tagcacagac aagtgtatgg gtaaatattt
     5221 tacttgtagg aattggagta attgcagtgc ttatatttaa accatctaga cactttagaa
     5281 agataatgaa taattttcca atcttgttta aagggtttat tgcatctatg gttggttgta
     5341 taattacact tttagttaat gattctggaa tagttgctgc atctacagca tctatatata
     5401 tacttatacc actaataata ataagtataa atatgataat atttgaaaaa aataactaaa
     5461 aaaattttta ctgttcatat aggatttaaa taaaaaatgc aatatgatta ttaatgtaaa
     5521 ctggatgctt acataaaaaa tcatattgca atttttatga ttgattatgt aaaattatat
     5581 gaagcagtaa gcctgtgacc ttagttatga gaggttcaga atatgtattt aatctataga
     5641 atataaatag ataaaggagg aactatgtta gatggaagaa taatgggtct tgatgttggt
     5701 gataaaacta ttggagttgc tgtgagtgat ttgatgggat taactgcaca aggtgtaaaa
     5761 acaataaaaa gagttggcaa gaaaaaagat attgaagagc taaaagcaat aataaaagaa
     5821 aaacaagtaa ataaaattgt atcaggatta ccaaaaaata tgaatggaac tttaggtcca
     5881 caaggagaaa aagtaatcaa gttttgtgaa ttggtaaaag cagaaacagg tattgatgta
     5941 gagttttggg atgagaggct ttctacagta gcggcagaga ggtctttatt agaagcagat
     6001 gttagtagac aaaaaagaaa aaaggttata gatatgttgg cagcagtaat aattttgcaa
     6061 ggatatttag attttaaaat aaattcataa aagaactata aaattgaata cttatgatat
     6121 ataatataaa taataaaaat aaaaataaat ttgaggtgaa tgacattatg gaagaaaata
     6181 taataaattt aatagacgaa aatggcgttg aaagccaatt tgaaataata ttaacattag
     6241 aagcagaagg aaaagagtat gcaatattaa tgcctttaga tgatgaagaa gctgaagaag
     6301 ctttaatctt tagaattgat gaggatgaag aaggtgaaat cttaatacct ttggaaagtg
     6361 atgaagagta tgaaactgtt gtagctgtgt atactgctat aatggaagaa gaaggtttaa
     6421 attacgacga agatgaatca aatggactta actagataag taattaagtt tgtatataaa
     6481 aatcagatat ttattatatt tgctttaatt aaacattatt aattgtaaaa gcatttatag
     6541 tatatatctg tttttttgtt ttttattaaa aataccaaaa aatttatgaa ttttggtgaa
     6601 taatatatct tttatgggta tatatttata ttatgagaat aaattaaatt tatttaaatg
     6661 tcttgacaaa aaatgaatta tcaattacaa tattatttaa taaagattat caattgaaaa
     6721 tagtaagggt gatgttatgg caaatacaat ggatttatta aaagacaagt taaaagaaac
     6781 tggttttaag attacgccac aaagaagagc aatagttgaa atattgctta aacatgacca
     6841 ttcacatcta agtagtgagg aaatctatga tttagttagg gttgactgtc ctgaaatagg
     6901 cttagctact gtatatagaa caatgcagct attggatgaa ataggattaa tatcaaaact
     6961 aaacttagac gatggatgta taagatatga gattagtcta cataaagaag actgtcataa
     7021 tcatcatcat cttatttgta aaaattgtgg aaagataatg gaagctaaag aagacctttt
     7081 agacaatata gaaaaagaga tacaatcatt gtacaaattt aaaatacttg atcatgatgt
     7141 taaattttat ggactatgtg acgagtgtaa tggagtaagc gattcagaag aataaacttc
     7201 tattgcttaa tgtcgatgga agaatagtat agaactttta tattctatgc tattcttcca
     7261 ttttttatta taaaccatct ataaaatgca aatatacagt tttcaagatg cttatagttt
     7321 taatattgaa aatataatta atcctataag tacaaaaatg aatgattttt ctatgaaatt
     7381 actaaatggt cctccaacag ttaaaaaacc taaagaaatt ttcttttcgc ttagcggata
     7441 aaaaattggt attccttgct ttgtaaataa atctcctaaa aacatatgac ttgcataacc
     7501 aatcgtacca taaaaagata aattattaat attagtgatt aattctattt gttttagtaa
     7561 aaaatatact attatagttg caaatatgct atgagaaaaa cttctgtgct ttaaccaggg
     7621 aaaaattgac aacattagac aaaaaattgt aaaatatatt tcaacttcta tgatgtataa
     7681 gcatatagct aatagtataa atattaatga tagaaaaact tttcttagaa atgtgtgggt
     7741 tagttttgcc tcaataatta taattgcaac aaatgtaatt attgcgctta aataaaaatt
     7801 atcatttata tttattgaaa taaagaaaat aataatatta acagcatata taaaaattct
     7861 atatatgtat ttggatacat tgttttttag aatgaaattt gagaccatag aattagattt
     7921 atccaaatcg ggtagtacag caaaagtagc tgaaactaaa atatcaacta aagatatagg
     7981 aattttaaat attaaagatg tttggatagc tgttaagatt cctattgcac aatgagtttt
     8041 accacgcata aatcctccta aattatccga tattaaatca tataatattt agaggatatc
     8101 aaaataaaat aggtttgtca aaataatacg ttaaatgtat tttaaatata gcttaaaata
     8161 tgatacgaat tatggtacaa taagaataac tggtagcgag tgcgtgattt gatattattt
     8221 atgattgtag aacgtgttaa aaaattaaat agacagaagg agatgatgca attgttcaaa
     8281 aagaacacca acaagataaa ggtaatggcc ttgggtggac ttaacgaagt tggaaaaaat
     8341 atgactgttg ttgagtataa agatgaaata attgttatag atgctggact tagttttcca
     8401 gaggatgaaa tgttaggagt agatatagtt ataccagata taacttattt agtaaaaaat
     8461 agggataaaa taaagggtat atttattaca catggacatg aagaccatat aggagctctt
     8521 ccatatatat taaagaaaat aaatgtacct gtatatgggg caagacttag tataggtctt
     8581 atacaagtaa aacttaaaga acataaaatg aataatgtta agttaaatgt tataggtcct
     8641 agacaagtta taaagcttga taatatggaa gtagagtttt tgaaaaataa ccatagtata
     8701 ccagatgctt actctattgc aatacacaca gaccaaggta taatatacca cacaggagat
     8761 tttaagattg acttgactcc tattgatgga gatgttatgg atatgcatag aatctgtgag
     8821 ttgagcaaaa aaggagttct tttaatgctg gcagatagta ctaatgcaga aaaaccagga
     8881 tttactatgt ctgaaaagac tgttggggta ggtcttgacg aattatttgc aaaaggaaat
     8941 ggcagaagaa ttatagttgc tacctttgca tccaacatac acagattaca acaaataata
     9001 aatactgctg aaaagtttaa tagaaaagtt gctatatcag gtcgctcaat ggtcaatgta
     9061 gttggagttg caaaagaatt aggataccta gatatttctg atgacatgct tatagattta
     9121 aatgatatat gtaagtatga agatagtgaa ttagtgataa taacaactgg ttcacaaggt
     9181 gaacctatgt cagctcttgc tagaatggct ttttcagagc acaaaaaagt agaaataaag
     9241 agtggcgatt tagttataat atctgctcat ccaataccag gaaatgaaaa attaatatca
     9301 agagtaatca atttcttatt tgaaaaagga gcagaagtag tatacagtga tatagctgat
     9361 atacacgttt ctggacatgc ctgtcaagaa gaattaaaac ttatacatgc attagttaga
     9421 ccaaagtttt ttatgccagc tcatggtgaa tatagaatgt taaaaagaca tgctgaaata
     9481 gctgaacagc ttggtatgga taaagaaaat atttttgtca tgcaaacagg agatgtttta
     9541 gaattagata aaaattctgc taaggtagca aaccgtatac aaacaggaaa catattagtt
     9601 gatggtctag gagtaggcga tgtaggtaat atagttttaa gggatagaaa gcatttatca
     9661 gaagatggtc ttatgatagt tgtagttact atttccaagg atgagggtaa ggtcttagca
     9721 ggtccagata taatttccag aggattcgta tatgtaagag agtcagaaga tttaatggat
     9781 ggagcaaagg atataattaa gaatgttcta aatgaatgtg aagagaaaaa tattaaagaa
     9841 tgggcttatt taaagaataa tattaaagaa aacctaaaag aatatttata tcaaaaaact
     9901 aaacgaaatc caatgatact tcctatcata atggaagtat aatataaaaa aactataaat
     9961 ttgaaaatta gacctcttgt aatttttaaa gtaatatcaa aaatccaatt gatattatct
    10021 taagaattac aagaggtctt tttaatactc attttaaaaa aatatatatt acataaaata
    10081 ccaattataa caatctttta ttagtaaaaa ctacttatat ataaatttta ctgataggag
    10141 gattcttatg gatgatgttt caagacaaaa tgctataaaa gctttaaaac aaacaaaaat
    10201 ggaaatagca ggagagtatg gaatgaatta tgaagatgct tttgaaataa tagaaaatgc
    10261 gtctaataaa ggtgttttag aaggttactt taaaaagctt gaaaagaaaa aaaatttagg
    10321 tcaaggtata tctaggcatt tagaataaaa aaagttaaat gtgagaaaat aagtttaaat
    10381 aaactttaag aggtgacatc gatgaaaaaa ttagctagta ctgctttggc tatattaatt
    10441 gcgcttactc ctttaagctt ttcttttgca aataataagg aaaatgctga tgccaaccaa
    10501 ctaaatatat cttcaaaatc agcaattttg atggatgttg gtagtggaca aattttatat
    10561 gaaaaggatg ctcataaaaa gcttcctcct gctagtgtaa ctaaggtaat gactatgcta
    10621 cttatagtag aagctttgga ttcaggcaag attaagcttg atgatgaagt tcaagtaagt
    10681 gaaactgcat ctagtatggg tggtagtcaa atattcctag agccaggaga aactcaaaag
    10741 gttgatacat tattaaaagg tatagctgtt gcttctgcaa atgatgcttg tgttgctatg
    10801 gctgaacact tagctggaag tgttgaagga tttgttgata gaatgaatgc taaagcaaaa
    10861 gagttaaata tgaatgatac tcattttgca aatacaaatg gacttccagt tgcaaaccac
    10921 tatacatctg ctcatgatat agctttaatg tctagagaac tattaaaaca cgaaatgata
    10981 agtaaatact taactacatg gatggataag gttgtagtag ggaagaaaca agttacagta
    11041 ggtcttgcaa atactaataa acttataaag cattatcaag gagcaactgg tgttaaaact
    11101 ggttttactc aagaagcaaa atactgttta tcagcatctg caaagagagg taatactcat
    11161 ctagttgctg caactttagg agcagagact tctccagaaa gatttaatga tgcctctagc
    11221 cttttaaatt atggatttgc taattatgaa agtgttaagt tatgtagtaa aggtgataat
    11281 atagcaacac ttacattgga taaggctgat gaaaacaaag ttaaattggt tgctaaagaa
    11341 gatttaaatg ctcttattaa aaaaggaagt tctaaagaat ttgagaagaa aattgaaata
    11401 gtaaaaaatc ctaagatgcc aattaaaaag ggaactgttt taggaaaaat aaagatatgt
    11461 aaagataaaa aagtaatagg cgaagtggaa ttgataaata ctaaggatat aaataaggca
    11521 agctatctac aaatgcttca aagaataatt gataatatga tataaataat tatcagatga
    11581 aatataatta taatacacaa agatatgatt ataatacaca taaaaaatac ccttatgtaa
    11641 ttatttgcca taggggtatt tttatttacc atatagaagt attttataat cttatgattt
    11701 ttatagaaat atagaaaagt ttatgtatct taaaatatca tttgatttaa tatcatatat
    11761 agggttgttt aacaattatc taagtaaagg catactacca gtaagtttat tcactttctt
    11821 tttattccca taaattgcta tcccaaaata ctgtaactca tcttctggaa cactagcaat
    11881 cttttgtgta aattcttcat aaagattgca actttgtgct acatctgaaa aatctacaac
    11941 agttaaatcg ttaaattcca aagtatataa ttttttacgt aaatctttta ttatttcttt
    12001 gtttcctttt aaaataggta caggaatgtc aataattcct aagtggctat tacctgtttt
    12061 gtcaattaca tctgggccaa cagtttctgg ggcatgtttt cctagtgtaa ttcccattat
    12121 agctgctgta ttggatataa ttcccattgg taaattttcg tctattacca tgacacattt
    12181 tgaattttta gtttccattt ttatttctcc tttttacctt atttaaagtt gtttgaatca
    12241 tcatttataa atatattttg atactgcttt ggtgtaagac caataaaatt cttaaaaaag
    12301 tttgtaaagt gactttggtc tacaaatcct gtttgtatcg cagcatctat aggttctacg
    12361 cctttttcta aaagcttttt agctttgttt acccgtatat tttctaggta tctatatggt
    12421 gtaatacctt ttaatttcgt aaaattacga agtagagagt acttattcat acctgctata
    12481 gtgcttaatt cgtcaagtac tatatgttca gcataattat tttctagata atcacatact
    12541 gcttgaattt ctatatttgt attttccaag ttagattgca aatttggctc tgtatgttct
    12601 tcaattaatt gttcaattag gaagaaaaaa agctcttcct ttttaaaatc caattcttct
    12661 tccatgatta tatagtgtaa ttcttgtagt agaggtacta gctcactgcg aaatataaca
    12721 ggttgattaa attttggtaa ataattttta cctgtaattt caaagacagt ttttttcatt
    12781 atttctggct taatattaat acatctgtaa tcaagtactt tatcatctat ttgttcacaa
    12841 gtatgactat caaaagggtt aaaaagtagc aaatctccag tacttgtggt atactcttta
    12901 tttttacaag ataaatatct ttgacctttt tctataaaac caattacata atgttcatga
    12961 aaatggtttg ggaatttttg cattatgcct ttaaatctat atgcttcaat ttttaattcc
    13021 ctatcaaagc atactgtgcg ttcttcttgt ctcacttagg taacctcctt tcatgttata
    13081 caaagtgtaa cacatatttt tttatacttc ttgtataata ttgactaaga gacaataatg
    13141 tcaattcatg tcaatataat attacactta cagtaggtgt aattattata ttttcacaat
    13201 gaatatgata taataataaa agttataaaa taaaaagcaa attttaattt tatgcctaga
    13261 agttaaaatt attataatgt aaaataattt tgattataag ctatttaatt gttataataa
    13321 tattaaaaat ttaatttagg agatgtacat taatgttaag tatgaatata ggagaaatag
    13381 tagcaagttt agttggtata gctatggcaa tatcaataca tgagtttggc catgcttatt
    13441 cagctcactt attgggagat gatacagcta aagcttatgg tagaatgaca ttaaatcctg
    13501 taagacatgt tgacccaata ggtcttttgg ctatgtttat ccttaagata gggtgggcaa
    13561 agccagtacc agtaaatcca aataacttta aaaactataa gataggaaac ctgatagttt
    13621 cacttgcagg cgttttttgt aatgttttga ctgctatact gtgtgtaatt atcaataagt
    13681 atgtgcatat gtatgctata aatacaatag ctgaatatgt gtttttatat agtattggat
    13741 ttgcagcttt taacttactt ccgatacctc cacttgatgg atggggagtt atatctacat
    13801 ttgtaccata taaatggaat gaatatttgt ataaatttga aagcatgagt tatataattt
    13861 tattaattgc tttatttact ggtatgtata gaataatatt aacaccaata tatgcagtgt
    13921 tgctaaaaat agtatattta tttgtttaat ataataaaca gattaaagag ggattgatat
    13981 gaagtataat atacaattac aagtctatga gggaccatta gatttacttt atgatttaat
    14041 aacaaagcat aaaatagata taaaggatat atccataata gatataacta agcagtatct
    14101 aaattatttg aaaatgctag ataaaatgga tttagagata actagtgaat ttataactat
    14161 ggcatcaaaa cttttagaga taaagtctaa atatctgctt tataagcaaa aagatgaaga
    14221 agaagaccca agaatagaac ttatggaaaa gttagaagaa tatagaaagt ttaaggtagc
    14281 atcacaagat ataaaagaaa atataactta tgttaatgag agattttata gaaataaaga
    14341 agagataatt attgatgaca atgtggattt agaagatata tcaatagaag caataaaaaa
    14401 tatacttcca tacatattta aagttaaaac atcacagata gaaaatgcta atgatgagaa
    14461 actagataaa atagtaagga agaaaataat atctgtag
//
LOCUS       DAMJYX010000086        14248 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMJYX010000086 DAMJYX010000000
VERSION     DAMJYX010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.088"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            150..1301
                     /locus_tag="PC479_002730"
     CDS             150..1301
                     /locus_tag="PC479_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805262.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1419..2057
                     /locus_tag="PC479_002731"
     CDS             1419..2057
                     /locus_tag="PC479_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2805263.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2058..3080
                     /locus_tag="PC479_002732"
     CDS             2058..3080
                     /locus_tag="PC479_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDF2805264.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3070..3690
                     /locus_tag="PC479_002733"
     CDS             3070..3690
                     /locus_tag="PC479_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805265.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3814..4500)
                     /locus_tag="PC479_002734"
     CDS             complement(3814..4500)
                     /locus_tag="PC479_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2805266.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4514..5059)
                     /locus_tag="PC479_002735"
     CDS             complement(4514..5059)
                     /locus_tag="PC479_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2805267.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5287..6534
                     /locus_tag="PC479_002736"
     CDS             5287..6534
                     /locus_tag="PC479_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2805268.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6621..8615
                     /locus_tag="PC479_002737"
     CDS             6621..8615
                     /locus_tag="PC479_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2805269.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8615..9937
                     /locus_tag="PC479_002738"
     CDS             8615..9937
                     /locus_tag="PC479_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2805270.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10216..11502
                     /locus_tag="PC479_002739"
     CDS             10216..11502
                     /locus_tag="PC479_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2805271.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11588..12895
                     /gene="grdB"
                     /locus_tag="PC479_002740"
     CDS             11588..12895
                     /gene="grdB"
                     /locus_tag="PC479_002740"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12635..12637,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2805272.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13172..13903
                     /locus_tag="PC479_002741"
     CDS             13172..13903
                     /locus_tag="PC479_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805273.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT         5704 a         1418 c         2452 g         4674 t
ORIGIN      
        1 ataaattagg tttatatttt ataaaaattt gctaaaaaaa acaattaaaa attgtaaaga
       61 aaaaagatat aattagaact gtagtaataa atgttttatt atagataata gagttttaag
      121 tatcttaaat aatatttgga ggaatatgaa tggaattaag accaagaagt gcaaaattag
      181 aagaatttga taaagtaata gaattaataa actatgtatt tagaatatca agaaatcata
      241 aaccaactat gatggaagaa tttcctcttt tactaagtaa aaataatata gaaaatatga
      301 ttataatatc agaagatgat aaagtgattt ctgatgtaaa ctatctaatt caggatgtat
      361 ctattcaagg aaacagattg aaggttgcag ctataggtgg agtatgtact catccagatt
      421 atgaaaagag aggctattca tctaagatat tggataaagt agaagaaaaa atgttttatg
      481 atggtgtaga tattgtaatt atatcaggaa caagaagtct atacagtaga agaaattgta
      541 gtttggtaaa gagtttttat aaatatacaa taaaacctga agatgtaaaa atagcatatg
      601 aaatagttga atttgatgaa actaattttg aaaaagataa tgatttagat aaaatgattg
      661 aactatataa tcaaaatagt actagattta taagaacgag agatgagttt caaaaactat
      721 tgcatgcagc aactattgcc tggggaccaa ttggttacaa gaaggtattt ataaaggaaa
      781 ataataatat aataggatat ttgataataa gaacaataaa aaaagaagat tctactgtag
      841 gagaagttgc tgaaataggg ttaaactctg taaatgttga aaatatattg aaatatgttg
      901 caaataaatt tggattagaa tatttaaatt ataaagtaca tgtgaaaaat cttaaagacc
      961 agttgaaatg taatgggaca aaatctttgg attatcaaca aggaactatg aaaataataa
     1021 attttactaa gttgtgtgat agtttaagaa gttattttag tcaatatgta gattttgaat
     1081 tactaaaata catggaattt aaacaagttg aaaataaata tattattaaa tacaaagaag
     1141 aagaacttgt aatagaaaat ttggataagc ttaataaact gttttttgaa aaaaatgagg
     1201 aacagtacaa tgaatttaaa catctaaaaa atatatatga atttgctaca aaggcatttc
     1261 ctgtagattt tccatggact gcaaatttaa attatcaata gttttagatg atattaaata
     1321 aaattgattg atacctagag ttagtccttg ttaaataaga gaaattattg gatattgaaa
     1381 aagcaatttt gtaagtaatt agaaaggatg agtattatat ggacatactt gatgatatag
     1441 cagaatgtat attaaatatg ggtgtagata atatagagaa tttagtaaaa attgctatag
     1501 ataaaaatat tgatgtggaa gatatttatg aatatggttt aaacaaaggt atgataggag
     1561 ctttagataa gtttgagaat aaggaatact atctttcaga agttatagta tgcacagatg
     1621 cattaaataa aggtattaat cttttgaagg gaactggaaa agtaaagaaa aaatcaaagg
     1681 gagtaattct gatgtctgta gtagaaggag atactcatga gataggaaaa aatattgtaa
     1741 aagtaatggt agaagccact ggatataagg ttattgactt aggtgtaaat agaaaaagtg
     1801 aagatataat agaagaagct ataaaaaaca atgtagatat aataggctta tcttcaatga
     1861 tgacaactac aatggaaaat atgaaatcgg ttatagacga acttaatatg attgagatag
     1921 ataaaagacc aaaagtaata attggtggag gacctgtaag tatggagttt gcagaagaaa
     1981 taggagcaga tggatacagt tcaaatgctc caaaagcagt taaattaata aataaactta
     2041 taggaggtga agtttaaatg ttttcagata tgactttact tcagtatata aatagtgaag
     2101 atagaggttt ttttctgcct gatatgggta caaatggatt gttcttaata aataaaaagg
     2161 catatgaagt atatgatagt gtagatttgc aattagaatt agcaaagact atggacaagt
     2221 attttgaatc agattttata tactcattct gtgatgggat tacattttgt gaaactctgg
     2281 gtttagagac attaagacca gattatgatt ttccaagtgt attgacacat actataactg
     2341 acagagataa attaagaaaa ttagatgttc cagaccctta tacgagtgga cgtatgccac
     2401 tcaatataga gagcttatca gttatatcaa aaagtattaa aaaaccacta tatgaatcga
     2461 tacaaggtcc atttacactt gcaggtcaat tagctggagc tactcaactt ttaagatgta
     2521 taataactga taaaaaattt gtagaagaat tattggaatt tacaactgaa ttggtaagaa
     2581 gatatgctgt agctgcaaat aaagcaggag caaaatatat ttcaatagca gagccaacat
     2641 cagtaaccct aagtaaagat agatttgatg aatttatagt aaaaaattta aataaaatct
     2701 acaatgaact tgattgttgg aaaggtatgc atatatgtgg agacaccaga gaattattag
     2761 ataatatgct aagttgtaat atagatgcag taagtttaga ccaaatttta gactatgaag
     2821 aaattgctcc aatgattcct agagatatag ttttgattgg taatttagac ccaataaaac
     2881 ttcttggacg ttctaagcca gataagatta gaagagagac gctaaaattg ttaaagaaga
     2941 tgagaagata tgataacttt ttatgtgatt ttggctgtaa ctgcttaaat accactccag
     3001 tagaaaattt acaagcagct ataaaggcag gaagaattag ttataaagaa ttagatagga
     3061 ttgatattga tgaattataa tagtaataaa aataaatatc ttttaaagga tataaattta
     3121 gatgatattt ttatagataa gaatattgtg ctaaagtttt tggggtatgg aaatagaaaa
     3181 gctccagata caattagtaa aatcgttgaa agagaaataa gaaatataaa tgatatttta
     3241 gatatcaaga tatatataag tgaagttgat attaataaaa cacctaaaga atgtaaaaaa
     3301 gcatttgctg tactatatac aataggggat aaaattgata ttaagatgaa tgactatatg
     3361 gaaaattgca atatgatggc agggctagca ttggataaaa taggtatagt atgtttagat
     3421 tatattaatg agaaaataaa aatgtattta aaaggaaagt atgatagtct caaaatatcg
     3481 catgaaatat atccaggaga taaagatttt gaagtagaga gacaaaagga tatttataac
     3541 tatgtaaaaa ataaatataa tagtatagaa atagaaatta atgattatca tcaactatcc
     3601 cccataaaaa gtgttgcaat gcttatttta atgggggatg aagaaaattt agaatcaaga
     3661 tgtagtaagt gtcctagaaa atgcttttag ttaattttca agactaagat ttgttttgtg
     3721 tataaattgc aataattttt ttggtcattt tatgtataaa ctaaaaaaat tattgcaaag
     3781 taatattcta tttaatttga acagacttac aaactatagt agtttcttta attcaataag
     3841 tgtatttatt tcataagttg gtatatgatt agtataattt atactgttat tggaatttaa
     3901 ccaacaagta tctattcctg aatttattcc acctaagaca tcagaggtta agctatcccc
     3961 aattattaag gcagaagatt tatcatgaca attaaacttt ttgagagcgt atttaaatat
     4021 ttcaggattt ggttttgata ttttcaactc ttcagataca accattccat caatatatcc
     4081 ttttattttt gaattttcaa gtcttttatt ttgaactgca gaaattccat ttgttattat
     4141 aacaatagta tacttttttt taagatattg taatatatcg attgcacctg gaattaaaaa
     4201 tgtacattca cctaaaaaat ttaagtacat tttgctaaga gtttcagaat caacatccaa
     4261 agatatttta tttgcaaaaa gttcaaatct aagtaattta agttcattca aagttataat
     4321 atttttttca aggtcgagcc aaagcttatc acttatattt ctatatgact ctatataatt
     4381 ctcaaaatta aattctaaat caaactcaga tagaagtttt ttaaaagcat gtgattcact
     4441 tttcttaaaa tcaaataaag tatcatctgc atcaaaaaat ataaatttat atgtcttcat
     4501 ggtttttact cctttacaag atgactgcaa aaattacttt actttcacta tttgagtcat
     4561 tttgccattt atgttttgtt cctaaaggaa cttttacact atctcctttg gacaaagtaa
     4621 attcatcatc atccataaaa agtttaactt ctccctccaa aacatatgca atttcatctc
     4681 cattatgagt aatcctatca acacaagaag aagtgtttgg agctaaatcc ataattgcaa
     4741 attctaaatt accactagag tctggaatta aaatctcata tataacttct ttgcttccag
     4801 gtaaaataac tttttttcta ttatcatgtc ttactatcaa gtttttttta tctacttcag
     4861 atacaaaaaa tgtaaataaa ggaacattta gagaagaggc tatagatttt aatgaattta
     4921 cagatggatt agcaagacct cgttctattt ggctcaataa tgatggtgta acatcagcta
     4981 gttttgctaa atctcgaata cttaagtttt gtttttttcg aacttcagct attttttccc
     5041 ctaaatttaa atcattcata taaatatcct ctctttttaa tttaaaaaat attaacttaa
     5101 aataatcaag taaaatgatt atgtagtatg aaattattat aacatataga attaggtttt
     5161 gaaaaaacta tgttcaatat aattaaataa attaaaatat aattgacaaa gtggatacag
     5221 aatacttaaa atatatttaa ataaaattaa gcaaaagata gttaatttat gataggaggg
     5281 gatattatgc aaataacgac ttttggagct atatttggtt tattaatagc aattatttta
     5341 attataaaaa aatttcaagc tgtatatagt ttaatgttag gagcatttat tggaggatta
     5401 gttggaggag caaatataac acaaactgta gattttatgg caaatggagc tatgaatata
     5461 tcaccatcaa ttcttagagc tttggcatca ggtgtattag ctggaagttt aataaaaaca
     5521 ggggcagtag ataaaatatc agaacagatt gtaaaaatat ttggtgaaaa gagggcttta
     5581 ttttcgattg ccatttcaac tatggtactt gctggagtag gtgtaaattt ggatgtatct
     5641 ataataactg tagcacctat aggattatat ataggtagaa aattaaatta ttctaaattg
     5701 tcaatactat tagctatgtt gggagggggt aaagcaggta atataatatc accaaatcca
     5761 aatactatag cagtagcaga taatttttct gtcaatctct ctagtgttat gatggcaaat
     5821 ataattccag caattattgg agttgttatt acagttattt tagcaagtat attaataaat
     5881 aaaggaaata aagtacaatc atatgaaatt ttagagcaaa gagaagattt accaagttta
     5941 tttaaatcat tatgtggtcc aattatagct atatttctat tgtttttagg aaatgtatct
     6001 cctatagtta tagacccaat gatagccctt ccaataggag gaatcgtaac tctaattgtc
     6061 acaggaaact tgaacaattc aagagaatat ttaacttttg gattatctaa gatgcaaggg
     6121 gtatgcatat tattacttgg aacagggact atcgcaggaa ttatacagat gtctgaatta
     6181 caacagagta caataggtgc tttacaattt ttaaatatgc cacaattttt attggcacca
     6241 gtttcgggta tacttatgtc attagcaaca gcatcatcaa cagctggtgc tactatagca
     6301 tcatctactt ttcatgatgc tataataaat ggtgggctat caccgatttc aggtgcatca
     6361 atagtaaatg ctgggtctag tgtattcgag caattaccac atggctcact atttcatact
     6421 agtgcaggta gtataaacat ggatataggt gaaagattta agttaattcc atatgaagca
     6481 ttgataggaa tagtaatgac tataatttca acatctattc aattagtttt ataataaata
     6541 taaatattta atgaaaaaag taggattttg ttattaaata ttgaacttat tatagtaaaa
     6601 aagaagattg gagttttaaa gtgaataaaa aaaagattgt tattataggg attatttatt
     6661 catttttagt agtattttca cttacaaata tgtatgtaaa tatggagtat aatctaaatg
     6721 tatttgaata tattaagaag tcacttccat ttacagaaga agaaaaaaag tggttagaaa
     6781 aacacaagaa tttgatttac agctctgacc aaagttcacc tcctttgaga tataaaggta
     6841 aggaagatgg acagtataag ggaattattg ttgatttaat aaattctctg tcaattcaaa
     6901 ttggtagaga tttttatttt aaaccaaata attggtggaa agaatctttc gtaaattcaa
     6961 tagatgatag tataaagttt tttgacttga ttccatcaaa agaaagggca aataaattta
     7021 tatttactga cccaatatac actttgagtg caaatatatt gaaagataaa aagtcccaag
     7081 atataaatag ttacatggat ttaaagggga aaacagtagc aattccagaa ggtgattatt
     7141 ctataaattt tttaaagcaa aaaattcaag atataaacat tttattgact ccagatataa
     7201 agactggagt caaccattta atgtctggaa aagtagacgc agttgttggt gatgaaccag
     7261 tattgcgata ctatataaat aattatggac tttcaaataa atacagtgtt ttaagtaatc
     7321 ctatatatac taaaaaagca gttcttgctg tacctaaaca atatgaagag ctggttagta
     7381 ttttaaataa aggcattttt aaacttcaga aaaatggagt gtataaagat ttaaagaaaa
     7441 aatggtattc tacgtataat gaagttgatg atatattgta tgagcgagga attgtgccta
     7501 gtatttatct ttttataggt ataattttaa tatcaattta tgttttttat agctatacat
     7561 atttgttaaa gatagaaatc aaaaggaaaa cagaacaggt aatagaaaat aaaaaaactt
     7621 tagaggcaac ttttaatagt attacagata taataatgct tgtagatgaa aataacaata
     7681 tagtagaatc gaacaaagta ctatatgact ttatgggtga aatgagttat aagatagcag
     7741 atttgattag catgataaaa ggtgttattg aaaatacctt ctctgaaaat actaataaaa
     7801 ctagtgaaat agaaatacac aataaaattt taaagataaa cacttttcct gtagagtata
     7861 aaaaaaataa tactgagtat atagtagttc ttattaaaga tataactaat gataaaatag
     7921 tagaggcgaa attacttaga gaaaacaaaa tgatatctat agggcaatta gcttctgggg
     7981 tagcacatga gataagaaat cctttgggta taataagaaa taattgctat ctcttaaaag
     8041 ataacgttac aatggaagaa gtaaatgatt gtgttaaatc aatagaaagt aatgtagata
     8101 gggcaagtaa tataataacc aacttgttaa atttcgcaag aatatcagat gataatttag
     8161 aacatataaa tataaaaaat tttattgaaa atatagtgaa acttcaatat aaaatgttac
     8221 agttaaagaa tgttgaaata aaaattgact gtgaacataa tttaatatgc tatataaatg
     8281 gagaatcgtt aaaacatgta tttataaatt taatttctaa ttcaatagat gcaatacatc
     8341 aagatggaaa aattattata tactgttatg aaaaaaatca ttgtttattt atagatttta
     8401 aagataatgg tgaaggaata aaagaagatg tcctaaagga catatttaat cccttctata
     8461 caacaaaacc aattggtgag ggaactggtt taggtttata tattacttat aatgagatta
     8521 aaaagaataa tggagatata agtgttgaaa gtaaattggg ggttggaact tgttttcata
     8581 taaaaattcc attaaataaa gaggtgacta tataatgaaa atactattag ttgatgatga
     8641 gctagaatat ggagtggtaa tgaaaaaaat tctacaaaag aagggctatc ttgtagatat
     8701 agcactaagt ggtgaagaag ctataaatat aattaaaaaa gataaaaatt atgatttggt
     8761 tttatctgat gttatgatga aaaatatgga tggagtacag ctattagata gaattaaatc
     8821 tattaataga gatatagagg ttatattagt tacaggatat ggaagtattg aaaatgcagt
     8881 tgatgctatg aaaagaggag ctttatctta ttttataaaa agtaatccca ttgaaaattt
     8941 attagaagaa gttgaaaagg taaaggcaag taaaatatct gtttctgtac aaaaaaataa
     9001 ttttgaattt actttggaat ctaaaaatag agattttaat aatatcataa aaatagcaaa
     9061 aaaagctgct tgtaaagatg ttaatatatt aatcttgggg gaatctggtg taggaaaaga
     9121 tatattagct agatatatac atagcataag tccaagaaag aatgagatat ttgtacctgt
     9181 aaattgttgt tctttttcag aaaatctatt ggagtctgag ctgtttggtc atgaaaaagg
     9241 ttcttttaca ggagcagtag atagtagaaa aggtagattt gaactttcga ataaaggaac
     9301 tctatttttg gatgaaatag gagatatacc tctaaatgtt caggtaaaac ttttgaggac
     9361 acttgaggac aaatctatag agaggatagg aagtaataaa tctattaaag ttgactttag
     9421 gttaatttgt gctatgaata aagaaccaaa agtggaaata tcaaatggaa atataagaga
     9481 agattttttt tatagaataa gtacaataac aataactata ccaccactaa gaaagagaag
     9541 agaagactta actactctta tagaattttt cttaaataag taccaaattg aacatgataa
     9601 aaagatacac tcgattgata aggaagtaaa ggatttttta ttaaattaca attatccagg
     9661 aaatataaga gaattaaaaa atataataaa tagacttgta gttttaagtg aagaaggtaa
     9721 tttatcaaaa gacaatttaa atttaatatc aaataatgta tacatagatg ataaaataag
     9781 tattagacct ttaagagaaa taagaaaaga gtttgaatgt gagtatattg aaaaagtttt
     9841 atctttgtgt ggaaataata tttcaaatac agctaaaaaa ttggagatta gtagaagaca
     9901 gttaaccaat aaaatatcag agtataatat taagtgagaa aaaaacttct cattaagtga
     9961 gaaaaaaatt tctcaaactg tattaaaaga taaacagaaa aaatctgttt atcttttttt
    10021 attataattt taaaaattaa tatttacaat aattattgct aaattcaata ttggtgtaaa
    10081 ttaaaaatga aaatttctat gacattaata catagaatat atattttaaa taattggcat
    10141 agaaaatgct ttatatatta ataaacgtaa taaatacttt taattgcgat taaatatatt
    10201 aagggggatt ttcatatgaa gctagagcta gggaatatat ttataaaaaa tgttgaattt
    10261 ggagaaaaga cagaagtaaa agatggagta ctatatatta gtagcgaaga aatagaaaag
    10321 atagtattag aagatgaaag gattgtaagt gtaaatgttg aacttgcaag acctggcgaa
    10381 tcaataagaa tagctccagt taaagatgta atagagccta gagttaaaat tggagatgaa
    10441 agcaaaatat ttcctggaat tataaataaa gtgaagactg taggaagtgg aagaacccat
    10501 gtacttttag gagcttgtgt tgtaacttgt ggaaatatag ttggatttca agaaggcgta
    10561 attgatatga gtggtcctac tgctaaatat acaccttttt caaaaacaaa taatatatgt
    10621 attgttataa aagcaaagga tggtatagat actcatgatt atgaagaagc agctagaatt
    10681 gcaggattaa aaataggtgc atatgtaggt gaggcaggaa gagaagttga gccagatgaa
    10741 gtcattgtat atgagacaaa gccattatta aaacaagtaa aagaatatcc agacctgcca
    10801 acagtagcat atgttcacat gttacaatca caaggacttt tacatgacac ttattactat
    10861 ggggtggatg ctaaacagat agtgcctaca tttatgtatc caactgaaat aatggatgga
    10921 gctataatat ctggaaactg tgtagcacca tgtgataagg ttacaacatt ccatcattta
    10981 aataatccag ttatacatga cttatataaa agacatggaa aagatttaaa ctttatagga
    11041 gttattttga ctaatgagaa cgtattctta gtcgataaag agagaagctc tgatatggta
    11101 gctaaattaa tagaattttt aggagttgat ggagttctag tgacagaaga aggttatggg
    11161 aatccagata ctgacctaat gatgaattgt agaaaatgta gtgaagttgg agcaaatgta
    11221 gttttgataa cggatgaatt tcctggaaaa gatggcaaga gtcaatctat agcagacgct
    11281 actaaggaag cagatgcagt tgtttcttgt ggtcaaggaa atctagtggt tcatttccca
    11341 gcaatggaaa aaattatagg aactttagat tatgttgaaa tgatgatagg tgggtataaa
    11401 ggatgcttaa atgaagacgg aagcatggat gcagagctac aaattataat agcatcaact
    11461 atagcaaatg gctataatca tttgacagca agatattatt aaataagtat ttgaatataa
    11521 agttctgttg atgtaatcat tggtctaact gatgtaataa ctataaagtg atttggagga
    11581 ttaagagatg agtaaaatca gagtggttca ttatataaac aatttctttg ctggtatagg
    11641 tggagaggaa aaagcagata ttccgccaga aaaaagggct ggtgtagtag gacctggaat
    11701 agcatttcag agtcaattta atgatgatgc agagataatc tcaactgtaa tttgcggaga
    11761 tacgtatttt ggtgaaaata ttgagagtgc aactcaagaa cttttaaaaa tgataaaaga
    11821 agaaagccct gatttattta ttgcaggccc agcatttaat gcaggtagat atggagtagc
    11881 ttgtgggact atttgtaagg cagttgaaga agagttaaat attcctgtaa ttacaggcat
    11941 gtataaagaa aatccaggtg tagatatgtt taaattagat ttgcatatag tgtctacagg
    12001 aaattcagca gcaagtctta gaaaggtagt tccaataatg acgaaactag gtcttaaatt
    12061 ggttagaggt gaggaaatag gacctcctga agaggaagga tatataatga gaggaataag
    12121 aaaaaatttc ttccatgaac ttagagggtc tgaaagagca attgatatgt tggttaaaaa
    12181 gatgaaaggg gaaagctttg aaactgagta tccaatgcct gaatttgata gagttacacc
    12241 tatgaaacct gttaaagatt tatctaagat aaaacttgcc ctagtaactt ctggagggat
    12301 agttccaatt gataatccag acaaaattga atcttcaagt gctactaaat atggaattta
    12361 cgatttaaca gatatggatt ctatgtcaaa taaagaattt acaactatac atggggggta
    12421 tgacagagca tatgtccttg aaaatccaaa tttagttgta ccactagatg tagttagaga
    12481 gcttgaaaaa gaaggtgtaa ttggagaatt agtagattat tttataacta ctacaggaac
    12541 aggaacgagt gttggcaatt ctaagagatt tggagaagag ttttctaaga aattgttaga
    12601 agatgatgta gatgctgtaa ttttaacttc tacatgaggt acttgtactc gttgtggagc
    12661 aacgatggta aaagagattg agagagctgg tatagcagtt gttcatatat gtacagtagt
    12721 accaatatca cttacaatag gagctaatag aattgttcca gcagtaggga taccatatcc
    12781 acttggaaat cctatgttag gagaagagga aagtaaaaaa attagaaaga atatagtttt
    12841 aaaagcttta aatgcactaa cagaagatat agaagaacaa acagtatttg aataataaaa
    12901 atacaacaca acactgaaat aaaaaaagat atcaaaaaat aattttttaa aattatttta
    12961 cgatatcttt ttttattagg taaattcgaa atttgcattt ttttaaatta aatttataaa
    13021 agtgataaat tagtttatta taatattgat acacaaattc aaattagata tactatgaaa
    13081 agcgagatta ttacattttg gaacaaatat tcaacatatt agatcatatt aattgaacag
    13141 aaaaaattaa ctttatataa taattagaat aatgataaga aagaaggtgt atatattgat
    13201 ttcaattgcc atttgtgatg atgaatccta tatgctaaat catttaaagg aaaatataga
    13261 attatacatg aagcagaaga atttattata taatatagag ctttttaata aagtagaaga
    13321 tttactatgc aatcatgaaa agtttaatat tgtattttta gatatacaga tggaagagat
    13381 taacggaatg caggcagcta gaaaattaag gtcatatggt aatgagtgct ttataatttt
    13441 cgttacagtt ttaaatgaat ttgtatttga tgcttttgaa gtagatgcta taaattatct
    13501 gataaaacct ctatctaatg agaaactata taaaactttg gataaaatag ttacaaaact
    13561 tgatgataat gaaagtaatt atatcacaat acaaaaaggt tattcatatt ggaaattaaa
    13621 acttgctgat attttatatt gtgaagttat aaaaagaaag atttatgttc atttaaagaa
    13681 agagactgta gattattatg agactattga aaatttagag agaagtttac caacaaattt
    13741 ttttaggtgt catcgtagtt atataatcaa tttacaatat gtatatggat atgaaaatgg
    13801 taatgcagtt atggaaagtg gagatagaat acctgtttct agactacgtc aacaaatatt
    13861 ttcaaaagtt atgcttgagt tcatgaaaaa taggaggata taagatgatt aatttatttt
    13921 cttccgattt atttttaaag tacagttttt tcttctcttt tattgtatat agaattttaa
    13981 tggtaatagt gcaatactat tttctaatca attttacaaa ttttaattca aaattttggt
    14041 gttttatttt atattcgata atttcaattt ttttaatcat tataaataca tattttaatt
    14101 tatataattt attttcttca cagattctat tttttcttat gactttttta tttgtaaagt
    14161 acataataaa acaatctact gttttatcta tagtatcaac tttgttagta tatttaattg
    14221 aacaattagt atatggattt acagctcc
//
LOCUS       DAMJYX010000087        13921 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJYX010000087 DAMJYX010000000
VERSION     DAMJYX010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.089"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(25..684)
                     /locus_tag="PC479_002742"
     CDS             complement(25..684)
                     /locus_tag="PC479_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2805274.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(901..2193)
                     /gene="lysA"
                     /locus_tag="PC479_002743"
     CDS             complement(901..2193)
                     /gene="lysA"
                     /locus_tag="PC479_002743"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2805275.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(2211..3404)
                     /locus_tag="PC479_002744"
     CDS             complement(2211..3404)
                     /locus_tag="PC479_002744"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2805276.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(3765..4382)
                     /locus_tag="PC479_002745"
     CDS             complement(3765..4382)
                     /locus_tag="PC479_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805277.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(4552..6252)
                     /locus_tag="PC479_002746"
     CDS             complement(4552..6252)
                     /locus_tag="PC479_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805278.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(6649..7212)
                     /locus_tag="PC479_002747"
     CDS             complement(6649..7212)
                     /locus_tag="PC479_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805279.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(7371..8084)
                     /locus_tag="PC479_002748"
     CDS             complement(7371..8084)
                     /locus_tag="PC479_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2805280.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            8376..10040
                     /locus_tag="PC479_002749"
     CDS             8376..10040
                     /locus_tag="PC479_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2805281.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(10147..11046)
                     /locus_tag="PC479_002750"
     CDS             complement(10147..11046)
                     /locus_tag="PC479_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2805282.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(11073..11711)
                     /locus_tag="PC479_002751"
     CDS             complement(11073..11711)
                     /locus_tag="PC479_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2805283.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(12048..13046)
                     /locus_tag="PC479_002752"
     CDS             complement(12048..13046)
                     /locus_tag="PC479_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2805284.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(13144..13719)
                     /locus_tag="PC479_002753"
     CDS             complement(13144..13719)
                     /locus_tag="PC479_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805285.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         4535 a         2374 c         1483 g         5529 t
ORIGIN      
        1 taaatttatg catatatttt atacttattg attatttgag ttttgagaat ttccaccact
       61 atctgaacca ccagtagtat cttgtggagt tggtgctggt gttggattac tattatttgg
      121 cttttcttga ttttgagaag aactgttatt attgtcagac ttattactat ctgatttttt
      181 atcattgttt ttagaactag atttattatt tttactactt ccactataag aagatttcct
      241 tgcatctgat tcaactctat acctatcttc tccagccgta tcttttgcct ttttctttct
      301 ttgttcctct gcttcgtttt gttctttttc taatttagtt aactcttctt tctttttagt
      361 tgcctcttct ttaataacag aggaaccatt tttgattttc tctataagtt taagttcctt
      421 gattgcaccc tcataatttt ttaattcttc aaactccgtc atatttgaca ttctcatagc
      481 ttgcatatac atagctcttg cattctcatt agtactatct tcttccagtg cagaggataa
      541 tgattccata gcttttttat agttatgttt ttctaaatat gtttttcctt gctctactaa
      601 atttatttca tcttttttat taaatgaaca tcctacaaga aatatagata atatcgatat
      661 taataaaatt actcttcctc tcattaaatc cctccttgtc atatacttta aattttatca
      721 taattataaa gtttttaaaa tattttatat ttattacaag aaatttgtta gtaaaacaaa
      781 aaataagcag atatgctacg aacgatacaa taaaccaaaa attaatttag agatacatat
      841 cctagcaaat ctgctcataa taaactttaa tatttaattt ttattatata cttctctatt
      901 ttatatctct aaagctaata agtcctcata gctttgtctc ttacaaatta tcttatcatt
      961 tccatcaaat actgatacca ctgctgctct tggtatttta ttataatttg aagacattga
     1021 atatccatat gcaccagttg atgttgttat aagtatatct ccacttttaa tatccattat
     1081 gtttatatct ccaattaata tatctccact ctcacaacat tttccagcta ttgttacatg
     1141 attcatagcc tcatgattaa ttttattcac aatactacac tcataactag cttggtacaa
     1201 cgaaggtctt acattatctg tcatacctcc atctacactt acataaaccc taacatcttt
     1261 tatatctttt atagaaccta ctgtatacaa tgtacttcct gcatttgcaa ctattgacct
     1321 tcctggttct attaccaatg taggtacttc tattccaagc tctttacatg aagtttctgc
     1381 tttagtgatt atggtttcac aaaactcttt tatagtttta ggtttatctc cttcgctata
     1441 ataaacgcca actcctccac ctaggtcaat ttctgttagt tgtatgttga atttttcttt
     1501 tatttctttt accaagttca tcattatatc cactgtatct atgtatggtt ctacatcaaa
     1561 tatttgtgaa cctatatgag cgtgaagacc tacaagttta atatttttat attcacttaa
     1621 tttttctatt gctctaaata aatctccatt tactaaagca aatccaaatt tagaatctat
     1681 ctgtccagtt tttatataat catgtgtatg agcttctata ccaggagtta ttctaaagta
     1741 tatctcttga gttttacctt tatcttcaca taatgcttca agcaaatcta attcataaaa
     1801 attatccact acaaatttac ctacacctaa atctaatccc atgtttattt cttctttagt
     1861 cttattgttt ccatgaaaga aaactttttc cataggaaaa tttgatttat aagctgtata
     1921 taactcgcct cctgaaacta catctaaaca catttgttct tcatttataa gattacacat
     1981 gtaaagaggt aaaaaagctt tccctgcata agcaacctta ttttttccct ctctagcctt
     2041 aaaatattct atatattctc tacaattttg tctaactaaa gcttcatcaa atacataaag
     2101 tggagtttga tattttttac taagttctaa acaactcact cctccaatat ataattcatc
     2161 attatttaca gtcatagttc catgaagttt cataatagcc tccccaaatt ttatatatta
     2221 aatacttctg ctattttttc tatagctact tgcttatcat cagaatttat agcacaagtt
     2281 atacgaatct ctgatgtagt tataagtttt atcattatac cattttcatt gaatatctta
     2341 aatactttag cagcaactcc agaagtattt ttcataccaa gacctactaa agaaaattta
     2401 gtgatattat tatcaatcac taaatgttct tcatctgtat atttagatac tatcttttta
     2461 cattctccca actcttcttt tggaactgta aatgatacac ttatcttatc taaaataggt
     2521 gcagtttggc ttatcatatc tacacttata cctatagttg ctatatcttc aaataagcta
     2581 gatatatttt cagctttaaa atcttttatt gttatagaac tgtcatcatc actagtagct
     2641 aatcctgtta ttactttatc ctctaatttc atgtcttttc cccctcttat atatgttcct
     2701 ttttctgtac cacatgtacg tccgacatag atttcaacac catatttttg tgcaagctct
     2761 atacttcttg aatgcattac ttgtgctcct aaactcgcta actcaagcat ttcttcatat
     2821 tcaatctcat caagtttact agcctttgaa tattttcttg ggtctgtaaa ataaattcca
     2881 tcaacatctg tatatatctc acattttcca tttaatttaa ccgcaagagc tacagcagat
     2941 gtatctgaac cacctcttcc aagagtggtt acatcaccat cttcgttgat tccctgaaat
     3001 ccagttacta tcacgacatt tccttcatct aaactatttt taattctgtt tacgtttata
     3061 tcatcaattt gagactttcc atgaagacca ctcgttttta tatttaactg gtaagcatta
     3121 taacttatag ctttacatcc aagtgcattt aatgctattg ataataaaga tgctgaaatc
     3181 atttcaccag tagacattaa agcatctaat tccctcttac taggttcatt actcaattcc
     3241 tttgctaaag ttatatattc atcagtggat tttcccatgg cagatacaac tattaccatt
     3301 tgtggatttt cttttcttcg ttctattata ttttctgcta ttgattttat tttttcagta
     3361 tctgctactg aacttccacc atatttttga acaattatac tcatgctttc tccccctcaa
     3421 gctaaatttt gataaataaa aacgcccaaa ggtatacttt ggacgtgtaa atttcataga
     3481 ccaagtaacc ttgtagctca ccactttaaa agtgacagcc ttatgcatat tctgcataag
     3541 tccagaaaat ttgtccgtca acaaaatttc ttcggctata taccctttca tcaattatca
     3601 acgcttacca gctcctattg aatactattg aatacagcta cctctaccct aggctcatta
     3661 tttttaatta atttattaat atagttttat catattctat ataatattgc aacaattatt
     3721 ttacaaaatg tggcaaatcg atagttttct tttctaaagt tagcttatat acaattaaat
     3781 agtttattta atcctaaatc tataaaattt atgctatact cattatcata tctctctaaa
     3841 tcatttaatt cggatagatt atctggtgta actaccaaaa cgtttggtac tttaaaaaat
     3901 ggaattgtag atttatctat ttttttagaa agattttcgt atttagaaat gtctatttca
     3961 ttatttaagt caacatcaac taaaacttgc tttatattac caaattggtc tgtatattca
     4021 ataaataaat cgtatttcac atcatctaca ctaacatttc ggtaaaatct ttttacatca
     4081 caacctgcag tattgattct aacaactaat tctgatccta ctagagcctt tattaaatca
     4141 tctcccttaa aaagtttact accgtttaaa tagtatacat aagagaggtc tttataatta
     4201 taataatcca catcacaagg atattttttt agtgtatatt cactacacat ttttctcaaa
     4261 gtttttttaa aatttttttt attgtatctt ttttggtctc cataaatttt tctaaattga
     4321 gactctgtaa cacatctaac cctgctaatt aaaagtaata tctctttttc catattcgtc
     4381 atagtacatt ctccttgaag tattatttgc aaagttgtac taatattata ttaattttat
     4441 gacattaaat ttacaaagaa ttaaaataaa atagctaatt tttatagaat taaattctat
     4501 aaaaattagc tatcactatg ttaaatacta ttatcaatct ttttatttaa attatacatt
     4561 accttgattt tcagaatctt tggaatctgt attttcatca gttcctgttt tttcttttgc
     4621 ttttttatct tcaaagtttg caatatttag gttatcaaat tctgacattt ctattacacc
     4681 agattcttct tttgaatacg atattttgac tttatcacca acctgagtca atggcaattc
     4741 gctagatact tttgaagtag ccctaaatat tatatcttta tttgaatcta aagttacata
     4801 ataattagtg ttaccacctc gcacatctgg gcttattctt gtcactgtgc cttctaatac
     4861 ctcatctttt aaatcattat caagttttaa atcattaccc ttagattcta atgcatcctt
     4921 atagtttctt aatgcttcat tcttatcttc acctattcca agtacattat aatcttcaac
     4981 agatacaaat gcaactctct taacaagacc cgccttatct tttaatgatg atacatatgt
     5041 tggctgaccc aatatattat acattacagg gaacgtcgct tcataatttt tttcttgaac
     5101 attaccttca gctgatttca ttgcagccgt ttcagtagct cctggttgtt tatataatct
     5161 agcttctttt gttctagaat caactaacat aaatccaatt gtagattcat ctccacctga
     5221 agatgttata cctgtatacc aataagacct gttatcattg ccataaacta atgatgtacc
     5281 ttctgttggt actaaaactc ccttttctga tataacagag tttaaaaatc cattaacata
     5341 aacaccccag tctttaattt ggtctgttac aaagctttgt ggttgtattc tatccaccca
     5401 tttaggcgtg tttttaacat cataaacatt tatttttcca gtttctgcat caacagtggc
     5461 tattcctgtt gcatttgctc ctccatagcc aattttatgt tcatataaac ttacaaccca
     5521 ataaggtctt ccttcatcat ttatttcaag agtgaaatct gtcataccaa catttacaat
     5581 accatgtatg tataaatgtc tttgtaaatc ctgatgtaag taggcttctg gttggtaaac
     5641 tatctttatt ggctttccat ctatttcttg aactaattgg acgtcttgag gattactagc
     5701 tgatactttt acataaccac ttgtaccact tagagatgtt atccatttaa ttatgtctct
     5761 gtgaacaaga ggcgctaccc agtacaactc tccatctaca ttttgtatat gaaattttcc
     5821 taatttggat acactcccta ttgcaggaac ttctccaagc tttttgtcac ctaatttcat
     5881 tgccatatct tcatctacaa gacgaatgtc atttacactg acagggctaa tgtcatcagt
     5941 aaatttactt tcagttactt ttcccaatag ttctctatat gattttgcat gaagtacagg
     6001 tgtagaagtt ataaagggaa ctactacaat atacacaagc aaagctattg ctattgaaaa
     6061 attatattta gccattttag atgctctttc atttctatct aacatcatat ctagtattcc
     6121 tgctacaaca aagaaaatta ttcctactga aaataaagat gtaaaatcta atcttagtac
     6181 aggtaattta ataaatgcat aaattattac aattattaat gcaactccat atgttttaag
     6241 atatcgtttc atttaatact cctctctaaa aaatgttatt attactatta tatccatata
     6301 tttacaaaaa ataaatattt atcatattta ttatatatta agatatcttt atatatttat
     6361 agttctgctt attcaactat atttaacatt atacgtgtat gtataaatac tttttataca
     6421 ctgtttgtgc tatccatccc gcacctaaag gagtgggatt tttgcactat tctgtattat
     6481 atatatccaa aaatgttata aaaatacaaa tattagcaga aatttaaaaa ctgaagaaag
     6541 tattctaact aagatttaca aattcttaat cctatcgaag ttaaactact atcttcagcc
     6601 taattatcta gcaatatttt tttatatgaa aaactctaaa tcaagttttt aaatgtttgc
     6661 gatttttgat acataagaat gttgtctaga attatattct gataataaac cttcatactc
     6721 taaggttaag ccagataaat tataaaaatc atttggcgtt acttcaaatg cttgtttatt
     6781 taaaatgtct atctcacttt tttctaacat gtcagcaaca aaatttgaac atacatattt
     6841 atattcagat tgtctctgaa tattcattag taaatatatt aatgccattg tatcgtaata
     6901 gtaatcttct ctataatcac taattaattt tatattttta tatagatttt catattgtaa
     6961 attatctact tctaacgaat ataccctaca cacagtgttt tttcttatag catataatcc
     7021 ttgatttata ttttcttcaa caaatcctcc aataaatgga ttccttggat ttagccttcc
     7081 aaaagaatac attggtttta gattttcatt taatgatatt gacacatgtg tatacagctt
     7141 tttacttata ttccttatta agtaagataa tatagttcct gtatatgttg ttacaatata
     7201 gactttattc atcatttccc cccaatctag taactaaata atattataca cttacaattt
     7261 aatatcataa gatattctaa cataataaat gtggttttac aaattttgat tttaaggtac
     7321 tttaatatta tataatataa taaaaatata aatataagtt ttctatattt ttaaccaaca
     7381 gatgttttct tcaaattaat cagatgattt agtatatctt gaacctttcc agagtccttt
     7441 aaatatccta atgccccatt tatttgtgat tttaaatcta tttctacttc catatccaaa
     7501 tcaacatttc ctttttttat agatatattt ttaagtgtta ttgggtaaag ataacttttt
     7561 tccaaagtaa tcttatttcc attaacctca aatggaactt ttttattgta gttacttaat
     7621 attttttcta gcattttatc acttagttta aattttccaa ttttcacatt ctcaagactt
     7681 acaactaagt ttccatcctt taaagtaggt ctcaattcaa gttcatattg actatttacc
     7741 aaaccaaata ccttatatgg tcctgctacc tttattttac catttttcat ttcaacaaaa
     7801 ttattttcaa gttcattaat tccatgcttc tgcatcaatg tatatattaa atttttaaac
     7861 tcttcctctg aaacagatat ttttccaacc attctaagtg gattttttac tacctccata
     7921 gaatttatat atgagccttt tgataaatat tcttcatcta tgattttatt attttgtgaa
     7981 ctaacatcat atctttcttt tggtgtcaag atatatacaa caattcctgc aagtactaaa
     8041 acaataacaa ttaaacttaa caaaatcttt gcaacctttc ccataatcgt ctcctttaca
     8101 aatctgtcat cagatttaaa ataaatttag ataactaact tcacataata ttcttaacaa
     8161 tatacatact ataatacagt cttttaaaaa ttaattcaac caatctaatg ttttgtaaat
     8221 aaactgtaaa aacatgtttt tagtataaaa gttaatattt tgcataatac tcttaattac
     8281 acctttattt ttttctaatc ttggattaaa attaatatta tgcaatacag tgtagtgttc
     8341 tgtattataa tcaaattaaa taggaggtat agaatatgtc aaatgaaacg aactcatcta
     8401 actttataaa gaatattatt ataaacgact tagaaacagg aaaacatgac agtatcataa
     8461 ctcgttttcc acctgagcca aacggatatt tacatattgg tcatgcaaaa agtatatgtc
     8521 ttaattttgg attagctaag gagtttaatg gaaaagctaa tttaagattt gatgatacaa
     8581 acccattaaa agaggatgtt gaatatgtag agtccataaa agaagatgta aagtggttag
     8641 gatttgattg gaacgagtta aattttgcat ctaactactt tgatgaaatg tataagagag
     8701 ctttaatact aataaaaaag ggtaaagctt atgtatgtga tttgacacaa gaggaaatga
     8761 gagaatatcg tggtactcta actgaaccag gaaaagaaag tcctcataga aacagaacta
     8821 ttgaagaaaa ccttgattta tttgaaagaa tgaaaaatgg tgaatttaaa gatggtgaaa
     8881 aaacattaag agccaaaata gatatgtctt ctccaaatat aaaccttaga gacccaatta
     8941 tatatagaat atctcattca acacaccata atacaggaga taaatggtgc atatatccta
     9001 tgtatgcttt tgctcatcca attgaagatg caattgaagg aataactcac tctatatgta
     9061 ctttagagtt tgaagaccaa agacctcttt atgattggtt tgttaaagaa tgtgagatgg
     9121 aaaatatacc tagacaaata gaatttgcta gacttaatat aaataatact gtcatgagta
     9181 aaaggaaatt aaaacaactt gtagatgagg gtattgttga tggttgggat gaccctcgtg
     9241 ttcctacaat atccggaatc agacgtaagg gatatactgc agaagcattg cgcaatttct
     9301 gtagtgaaat aggggtatca aaagttaact ctacggtaga tagtcaaatg cttgattatt
     9361 tcctaagaga aaacttacaa ccaaaggctc ctcttactat gggagtttta agacctttaa
     9421 aacttattat aactaattac ccagaagata agattgagat gcttgaaata gagaataatg
     9481 ctaaagatga atctcaagga aagagattag ttcctttctc tagagaattg tatatagaac
     9541 aagatgattt tatggaagaa cctgttaaga aatattttag atttttccca ggaaatgaag
     9601 tccgtttaaa aggtgcttat tttgtaaaat gtactgatgt aattaaggat gaaaatggaa
     9661 atgtagttga aatacatggt acttatgacc ctgaaactaa gagtggctct ggctttacag
     9721 gacgtaaggt aaaatctaca atacattggg ttgatgctaa atcagctata ccatgtgaat
     9781 ttagattatt tgaaccatta attcttgatg atatacctga aaatgaaggt aaacatttct
     9841 taaaacaaat aaatcctaat tcattggaaa tattacaagg ttttgttgaa cctacacaga
     9901 taaaagatgc taaacctttt gataaattcc aatttgttag aaatggattc tttagtatag
     9961 ataataaata tacaactgat gaaaaatttg tatttaatag aatagttcct ctaaaaagtt
    10021 cttttaaacc aggtaaataa tcttacaatc aaaaaatgaa gacttaaaat ataaattact
    10081 tctactataa ataaacttag ttagaagtaa ttttttattt gagtcttcat catttacttt
    10141 aaatgattaa cttttttcac tacagattcc catgacttct attccatctt ttatgcattc
    10201 tatgttaaga ggaaaatctg gtcctttttc tccgtcaata tctgttataa gtgaatcttc
    10261 acattctata tgcacctttt tagttttaaa gtatagtatt tcatttccat tatattgatc
    10321 taaataatca cctctaagca cacttatcaa aactggtatt gattttggta ttggcattcc
    10381 tttaaaaata attacatcca aatatccatc atctatttca gccttatatg ctagatttat
    10441 atttcctgct gttttgccat taaaaatcaa cataagatac atatctccat cataatacat
    10501 ttcctcagaa gatactttta tcttaaattt cctcatatac aatgcttctt ctattccctt
    10561 tatataataa gaaattcttc caaatgaatt ttttaagtct gtgtttatcc tttgtgatac
    10621 atccgtaaac ataccagcac ttgcaacatt tataaagtac ttattattga ctttccctat
    10681 gtctattttc tttggttttg aatttatgat ttcatttata gcttctttta tatcaaatga
    10741 aatttgaaga gcatttgaaa aatcatttgc agttcctgta ggcaaaattc caattggaat
    10801 ttttatgtct aattctttca ttgcatttac tactaaatct acagtaccat caccaccagc
    10861 tatcaatatg tgttcataag aactatcaac ttccttaaat gcattcttta caggttcttt
    10921 aatagttaat ctatagggta tcaataaata atttttttct tgatataatt ctatgatagt
    10981 gtctaaattt gataatatct ttttttcacc tgaattagga ttgtatataa gttttacttt
    11041 tttcattatt tctccttact cattttattc ttttagtatc caaaatcttc atttaaaaaa
    11101 atcacatgta ttctttctgg tgttaattgt ctttttataa gtgtataact acagcatatt
    11161 cttcctggac ttgaacagtc tatacatata ccagtagtct tacatccagt atttgtactt
    11221 aatcttttac aattggctgg agcagctatt gtttttgttc tttttatagc ttcatctaca
    11281 tctttaacta ttttatttac tcctgctata actattactt tatcaggtcc atatatcata
    11341 gcagcaactc tattcccatt cccatctaaa ttgtaaagtt ctccatcctc tgttatcgca
    11401 ttagtacttg taaaataagc atctgcagaa aatgactttc tatatatttc tttcatttct
    11461 tctggtttga tatcttttgc atatctatca agaaaattat atttttctct tataaaatca
    11521 atcactcctg tttcaaataa ggtcatagaa cctccacatc ctactagaga acctgcttta
    11581 actatttctt caattttttt aagaagctct tctttgttac ttactaaata ccctttcata
    11641 ttattgtttt ccaaagattt tattgttttt tctattcttt tttcattaag ccaatttaaa
    11701 ttttgatcca ttttaacttc ccccttattt ttaatatgca cttaattatt tataaacttt
    11761 attttcttta taagtttatt ttagtacatc taaaaaaaaa tacaatataa aacttaaata
    11821 gttttacata attttttcca aaataactta ctcaatcaaa aaaggaaagt agatgtacta
    11881 ttctactttc cctatattgt atgtagtatt tattgcctaa aatctatata ttaattatat
    11941 gtttcatatt atataatatc acagttaatt aaaatgtaat actatcttct ttttaaacat
    12001 ataaattgta ttattaaatt taatatatcc aacttcaata aatcttatta atttgatgaa
    12061 gaagatgcta tagctgcact tgttgctgcc attacacatg caatttgaat agcaacaaca
    12121 atatttaaaa atatattatt agttatagag cctatattat tctcatttcg tatcaaatct
    12181 gcataaattg aagctacaat agcagaacta atcattactc tattactttt tcctaaagac
    12241 caatttccaa agcctttttc ttctttaagt ttatttgaaa cattttttat ctcttctatt
    12301 atttcatctt ctctataatc taataatgaa attgctccta ttattggata tccataataa
    12361 ttcattttac aatcattttc tttaaatgct tttttaagtt caatagtctt tttacattta
    12421 agcatagttc tatcatcact aaataaaaga atttgggata atgcttgtaa atcatttcct
    12481 ttataaaaac cattttcatt tagaaattca taacattttt ctatgtattc taaatcttca
    12541 tctaaatttt ttgaatgaat tgcaataagt gctatattac aatagtcatc actagaagtt
    12601 agaaatggat gttttttcct catgtaatca tatattattt tcatttttga tatacatgta
    12661 agataatcca ttttctctct attttcataa actattgatg ctgtaagtgg taaatatgta
    12721 tcgccccaaa acttttcact tttaagttct ttgtatatat ctaaaatcat cttaaaacta
    12781 ctttctggat ttgattcaaa cgaaagtagt gttgccatat aaaacatatt atatcctcta
    12841 aaattagaga ataatccagt atttttttta ataagctcta ttcgttcttc cactaatcta
    12901 ggacttattc tcttgttctt aagggtaaat gcaagtgcac aactatgttg tatcaatccc
    12961 atttgccaag aacctttaac ttcatttaat gctttgtaat tttctattaa taagtttact
    13021 tttccattta taaacgattg ttccatattg ccctcctagt aatatatctc attgtactaa
    13081 tagttttata tttattatat atatattaaa tactacaatc aatacatatt acttcaaaga
    13141 acatcattct tcaacaataa tgtagccttt tttgtaatgt cttcccatat tattgactct
    13201 atcatagtac tctgattcac tttcattact ccatttgggc acaaatacat ctattgcatt
    13261 agaattacta aaatacttac tagaccccct atccataaca gtttttaatt ttccatctac
    13321 aataatctta gttccaaaac ttaagtgatt gtttgctaca aaaccatctt ctaaatcatc
    13381 tcctgtagca gttatcgctc catatccatt ttcttgatta agacttgtgt aataactaac
    13441 ttcaaaagtt ataggtacta acttctttat tttaatacct aactgatttt gtagtttctc
    13501 cttttgtact ttcaacttat actcatcact atttttataa tcttccatac cttgattata
    13561 cttggaatta aagcttttac ctgactttac tatagtatct atatttacta tagattcttt
    13621 acaatgatct gcatctattt ttgtttctcc atttaccaat aatggagtta aacatacact
    13681 acatacaaca catgtaatca atgcttcttt aagcttcata aattcccccc ttattatttt
    13741 gatatattag catatttaat attatatgtc tacatttaga tttaaatacc acacccattt
    13801 gctttaagaa gttgtcttat atttaattca ccttaaacac ttataaatcc tctatcttag
    13861 aatcatatag aataaagctt ttatctttaa atatttttta ttaatctttt aaaatctaaa
    13921 a
//
LOCUS       DAMJYX010000088        13518 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJYX010000088 DAMJYX010000000
VERSION     DAMJYX010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.090"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            852..1514
                     /locus_tag="PC479_002754"
     CDS             852..1514
                     /locus_tag="PC479_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805286.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1523..4030
                     /locus_tag="PC479_002755"
     CDS             1523..4030
                     /locus_tag="PC479_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805287.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4361..4705
                     /locus_tag="PC479_002756"
     CDS             4361..4705
                     /locus_tag="PC479_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805288.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            4943..5629
                     /locus_tag="PC479_002757"
     CDS             4943..5629
                     /locus_tag="PC479_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805289.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
     gene            5640..6881
                     /locus_tag="PC479_002758"
     CDS             5640..6881
                     /locus_tag="PC479_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2805290.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            6952..7635
                     /locus_tag="PC479_002759"
     CDS             6952..7635
                     /locus_tag="PC479_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805291.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            7632..9965
                     /locus_tag="PC479_002760"
     CDS             7632..9965
                     /locus_tag="PC479_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2805292.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(10073..10635)
                     /locus_tag="PC479_002761"
                     /pseudo
     CDS             complement(10073..10635)
                     /locus_tag="PC479_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10749..12098)
                     /locus_tag="PC479_002762"
     CDS             complement(10749..12098)
                     /locus_tag="PC479_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2805293.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            complement(12135..13355)
                     /locus_tag="PC479_002763"
     CDS             complement(12135..13355)
                     /locus_tag="PC479_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805294.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
BASE COUNT         5346 a         1531 c         2041 g         4600 t
ORIGIN      
        1 atgaaagttt agttttaatt catttattta ataaacattt caagtaaaat aatgtttaaa
       61 tcagaaatat aaataaatat tattataatc gttattaaag accaaaaatc ccaccttaga
      121 agtttgtatc atatgaaacc tttatagatt atgaaagcaa taatatagtg tatgatatat
      181 aaaataagtt ataaaagtat ttttttagat attcaaaaag acattgtaag ttatattaat
      241 aatatgggta tcaacgaatt ttggatataa tgagtttttc aacttaaagt tatgtaataa
      301 tgaatgtaaa gaatatttaa tgaaatcatt ttgttggtta tgtctagctg aatacaataa
      361 tcttttagat aaagatttta tatttgaaga taggtgtgaa aaaattaaga agatattaac
      421 atcagcagtt tagaggcaaa gcatgtaaag taaagtctgg gtaactaaaa gtagtaattt
      481 agattgtaac tattgttata aatatgaaaa tgaggaaaca ggaaatataa gttaggatgt
      541 agaaaatagg ttactaaata aaaagcttaa ttatataatc aaagaaatag gtatatactg
      601 tacaatttca tggtgaagaa cttttcctaa ataaaaaggt tggtaaaaga gatataagaa
      661 ataaaattca attataagat atggtatgat aactaaaggc actatatgga gtgaataggc
      721 ggaagaattt tttgaaaatt ataaaaatag tttctgttgc tacatatcaa taagtataga
      781 tagagattgc aaaatccata gcaaaaatcg aatttataag tgtaaataac atgtaagaca
      841 aggagtaaat tatgagtatt ttagagataa aaaacctaaa taaaatatat ggtaaatcgg
      901 aaacagaggt gaaagcgtta agtcaaataa atctaaaaat aaatagtgga gaatttgttg
      961 tgatagtagg taaaagtgga agtgggaaaa gtactttact acacataatg gcaggacttg
     1021 aaactccaac aaatggtgat gtgattattg ataatataaa tatatcatct cttgatgaaa
     1081 agaaaagatc tgcattacgt aaagaaaagt tgggattaat atttcaatca tataatttga
     1141 ttccagtttt aactgttgaa gaaaatataa aactaccaac tataaatata aaaaataaag
     1201 atgaagctta tattaatgaa ttgatggagt tactaggaat aaaagataga agaatgtatt
     1261 tgccaaatca attatcaggt gggcaacaac aaagagtagc aatagcaagg gctcttgtaa
     1321 ataaaccatc aattgtattt gctgatgaac ctactggaaa tttagacaca aagacagagt
     1381 cagatgtatt aagtttatta aaagaatctc aaaaaaaata taatcagaca ataataatga
     1441 taacacataa tatagatata acaaaatatg ctgatagagt tatagaaata gaagatggat
     1501 ttataagaga gtaggtagat ttatgaaact taattatatt gtaaaaaaat atataatggg
     1561 aaataagaaa aatacattat taataattgt aagtattgta atttcaacag cgttgttttt
     1621 aattatgaat ataataagtg aagatgcgac aaacttaatg ataaatcaag ctaaaaatga
     1681 atttacatta aaacatgcac aatatatcaa tccaaaagac gaagaaataa aatatataga
     1741 aaataacaca agtatagata aagttggaaa atcaatgctt ttaggaatta gtgatattgg
     1801 taaaggtcaa acactacaaa ttctatctca ggataaagtt gctgaagaac taaatgatat
     1861 ttacacacta gaaaaaggta aattaccagt agctgaaaat gaaatcgcta tagattcatg
     1921 gtatattaaa caaaaaaaga taaaaaaccc tataggaaaa aggataacat tagattatag
     1981 aagacaaggg atagatcaag aagatttata tactggggaa aaagaattta aaataactgg
     2041 tatcttaaat agtaatccta ttttaaaagc tcaaggaaca tcaattggcc ttataagtaa
     2101 cgattgtgct atcaagaata ttcctactaa aaataaatat gatcaagtta tgtttacttt
     2161 taaaaaggaa aaaaatatac agagacaatc acaaaaactt attaaaaatg gaaatttgaa
     2221 tgagaataac atttatttta ataatgaatt attaatagct atatctgata gtatgaattt
     2281 aaaaatacct tatataatag ttaatatagt actagcacta gcaactatat tattaattta
     2341 taatattttt tatatattag tatcaaatag aataaaggat tttgggatat ttagggcttt
     2401 agggtttaat ccaaatgata tttttaaaat aatgatttta gaagtatcta tatactctat
     2461 tataagtatt tctataggtc taattttagg tgggataata gctagtttat caagggagta
     2521 tgttatagga gtaatataca atgtcaacta tataaattct ataaaaaacc agaactatat
     2581 aaacacatac ataatttcta tattattaag tttaggaact ataataatat cagtttcaaa
     2641 acctcttatg ttatcagtta aaactgatcc aatgatttgt ataagaagaa ataatgaaaa
     2701 agtggatata aaacaaaatt cctttataaa taaatttatg ataaaacttt tcaaagacta
     2761 tggtaacata gcatcaaaaa atatacaaag aaataagaaa cgtacaaatt tatctatagc
     2821 atcaatggta attatatttt tcttaatggc aacaatatac acaaagtcta ctagtaactt
     2881 tttaagtgat ggtgtattaa aatattggat acaaggagat tatttattac ataatattga
     2941 tataagtact atacgttcaa ataataaagg ttatgataaa aatgtactac aagagataaa
     3001 ggcaattgat ggtgtaacaa aagtaaactc atataggcat aagtggttta atataaaaat
     3061 tgatgataaa agtataaata agaactctga ttattggaaa aaaaataaag ataatattga
     3121 agctagatca gaagtaaaag atggaattaa ggtatataat aattcttttg aatttttagg
     3181 aatagaagat acagatatac tagaggattt cttgataaat ggaaaggaaa atatagataa
     3241 atttagtaaa caaccatatc tgtatataac aaagaaatcc agtgattcgc ttaatataaa
     3301 aaaaggagat agaataaaag tatactttga tattatagat tcaaaaacaa ataattttac
     3361 caaaacaata tctcaagaat ttatagtatg tggaattata actacattac cagtaacctc
     3421 acaagtggga gctgaatttg gcggagtaat atcaacaaat caatttaata agtttacagg
     3481 aatatcatct tatgaaagat ttgacatatg gacgagtaaa ctagccaatg ataaatatgt
     3541 tgaaagtgag ttaaacaaaa taacagaaaa aacaaataaa ggaattttaa ttccatacaa
     3601 agctgaaaca gctgaatatg aaaaaattga taatcaaaaa acactaatta tggtattagt
     3661 aactggaatt atagttatat tatctttatt taattgttgc aatactatag ttactagtat
     3721 taatagcaga agtagggaat ttgcactatt tagaggaata ggaatatcaa aacatgaaat
     3781 aaataaaatt gttaaattgg aaagttatat atatattata gtaagctttt gtatatctat
     3841 aatacctatt ttaattgtaa gaagtataat aataaaacct tttgaaagta tcaatttaat
     3901 aaattggaaa tttatagggg ctattatact aatagtattt attttagtat ctataatcat
     3961 gataactaca ctaaaaacat tgaataaaat tcaaaatgaa aattttatgg aagaaataaa
     4021 gactttatag tgaacagcct ataaattaaa gaggttttat atgaatttaa ttgcaattat
     4081 cgataatggt gtagatattg attttaaata atttagaaat aaaaataaaa taagttatcc
     4141 aagttatatg tatgatgtaa taggggtaca atgtatagca aatataaata ttggcttaat
     4201 aaaactctac tcttaagtct actgtcgttt taacacaaga ctaataataa gtgtaaccta
     4261 aaaattattg ataatacaat aaccttgttt atgataaata acaaatatta agtgttgtaa
     4321 agatagtaag tttaaaaaaa tatcaaagat ttcaatcaca atgtattgtt tatctgtagt
     4381 gttagctatt tatggtatat atgtatttat aaattgtact gaatatatat atactatgct
     4441 atctacaaaa catttaacag tcaaaggaaa tgaatatacc attataaatt attatatgag
     4501 taactttagt cagtatataa tatttgctat tttacttgca tcaatgggat ttgtaataca
     4561 aaagatagtg catatgcatt tcagtgagat agaacttagt ggtaactatt caacaactgt
     4621 tagaaatata agtgatatct ctttagataa tgataatgaa atgtttaata gaggcaatat
     4681 tgaaaatagt attttaaaaa aataaataat ataaatatat aaaatgtgat tgaataaagc
     4741 ttagaatact tagtgtattt taagcttttt ttattattaa gatttcaaag tattaaatta
     4801 actatataaa atcatataaa aataatttta aaatatttaa atcattgatt tcaaggtatt
     4861 gtatttttaa aagagtaaaa atataataag ttaggtatat aatatatata aagtaaaaaa
     4921 taaaaaaatg gaagtgattg agatgagtag tatattgttg gttgaagatg atttaagttt
     4981 gatatatgga ttagaatatt ctatacaaaa gaatggattt agtgttgatg tagcaagaac
     5041 agtagaagaa gcattgcaaa tttataaaga aaaaaattat gatttactgc ttttggatgt
     5101 atccttacct gatggtgatg gttttgatat ttgtaaaagg gtaagagaag cttctaatgt
     5161 accaatcata tttttaactg cttctgatga agaagtaaat gtagtgatgg gacttgatat
     5221 gggtggagat gattacataa caaaaccttt taaattaaat gagttaattt caagaataaa
     5281 agctttactt agacgttata attttacaag tgaaaatgta actgaactaa aatcaaataa
     5341 tattactgtt aaattacttg aaaatcgtgt ttttaaaaat gaaattgagc ttgaacttac
     5401 tacagcagaa tataaattat tatgcttact tatgaaaaat aaaaatattg tacttacaag
     5461 aaaaaatata ttggataagt tatgggatgg aaatggtagt tttgtagatg ataatactct
     5521 gtcagtatat gtaagaaggt taagaaataa gattgaagat aatcctgaaa atccaaaaaa
     5581 tctattaaca gttagaagaa tgggttacaa atggaatgtt ataaagtgag ggtgttaata
     5641 tgaaggtatt ttctaataaa gacataaaaa tatttttttt ggtgattata tccgttttat
     5701 tgatgtttat agtagtaggt caaatagtag ttataaatat aactaatgat tataaaagtg
     5761 cactgttaga acatgattat aatatagctg gatacttaaa tagtatgggt gttgataaat
     5821 caaaaatacc agcagtattt actactaatg aagataaata tactttaaaa ggaaaagaaa
     5881 tcttaaatac agctagatat gactttggta cagatgatag ttttatgcca agtgtgaaat
     5941 tgtttcaagg taaatatata aaaactatgt ttttgtattt atttgtgttc tttactatga
     6001 ttatttttat attgtatgtg tattttttta ggcaacagaa caagatagaa gaagctagtt
     6061 ataaaattga ttcatttatg aatggaaata taaatgtacg gttaaatact tatgaagaag
     6121 gtagtttatc aaagttattt ggttcaatag atgctatgtc aacatctcta aacacacata
     6181 tattgaaaga aaagcagaat aaagaatttc ttaaaaatac aatttcagat atatcacatc
     6241 aactaaaaac tccacttaca gcattgatga tgtataatca aatattgcag gaagagagtc
     6301 ataatagtga aattgttgag aattttgtgc agaagagtca aaatgaatta gagcgtatag
     6361 aatcattgat acaaaacttg ttaaagatta caaagataga ttcaaagaca ataattctta
     6421 acaaagctag tgttaacgta aaaaagttga ttactaaaat tttatatagt tttgaaacta
     6481 gagcagaaaa agaaggaaag agtatctcta taagaggtct agaaaataca aacttgtttt
     6541 gcgattatga atggatatca gaagctttaa gcaaccttat aaaaaatgca ttagataata
     6601 caaaagagaa cgataaaata atgatagaat gggttgaaac acctataaca actattattt
     6661 cagtgagtga taccggaaat ggaattcata ttgaagatat acatcatata tttaaaagat
     6721 tttataggag taagttttca aaaagtaatc aaggccttgg attagggttg ccactagtta
     6781 aatctatcat agaacagcac aatggaacca taactgtaga aagtaatttt ttacaaggaa
     6841 gtacatttac tctaagtttt ttaaagctta ctaatatgta agataggata catcttacag
     6901 taaggtgtat gaattattct tatattaatc atcagaaagg agatagagca aatggatatt
     6961 cttgtggtta ataatttatg taaaacatat ggaaatggat atacaaaagt agaggcatta
     7021 aaagatgtat catttacagt ttcaaaggga gagtttgtta ctgtagttgg agaatctggc
     7081 tcaggtaaga gtacattatt aaatctttta ggaggtcttg atactccaac ctcaggaaaa
     7141 atatttgttg atggtaaaga aatatgttca atgaaagaag aaaaacagac tatttttcga
     7201 cgtcgtaata taggttttat ctttcaatca tataatctta tacccgaatt aactgtggaa
     7261 caaaatataa tatttccttt actactagat tacaaaaaac cagacactat ttatctagat
     7321 gaagtattga caattttagg tcttaaagat agaaaaagtc atcttccaaa tcaattatct
     7381 ggtggtcagc aacaaagggt tgcaattgga agagcattga ttacaaagcc atcaattata
     7441 ttggcagatg agccaacagg aaatcttgac agtaaaaata gcagtgatgt aatttcactt
     7501 ttaaaagtga cagcagaaaa atataaccaa actattatta tgataactca taatacaaat
     7561 atagcttcta cagcagatag gatactacag gtttctgatg gaatgcttaa agacctaggg
     7621 gtgaatagag aatgagaagc tatttagaac tagtaccaca atatgcaaag gtacataaga
     7681 aaaaaaatag aactacaatt ttatgtattg tcattgcagt atgtttagtt actgctattt
     7741 ttggactagc agacatggct atacaatgtc aaaaagtaca atttataaaa gatgatggaa
     7801 attggcacat agtacttaaa aatcttaatg aagaagctat atcagaaata agtagtagag
     7861 tagatatagc tcactatggt agaggacaag tcattgataa tggagagttt aaatcaaagg
     7921 agattttgat acaaggttta gatgaagatt tagcaaaaca attaggtcta gaaataataa
     7981 aaggtcgtta tcctgttgtg gagaatgaga ctttaataga tgtagatgca gttaaaagct
     8041 ttaatctaaa agtaggagat tttatcaaaa taaaattagc agatggtaga aataaaaatt
     8101 ataagatagt aggtgaatat tcatcattat caagattaag agcctcagat aagcatggat
     8161 taatgctttc aatattaggt acacaagcta tcaaaaaatc agaaacatat gattattatt
     8221 atattacatt taaagacaag gttaatatta gaaaagctac ttctgatatt aagaatacct
     8281 tcaatttaag tgataaacaa attggtaaaa atgaaaaatt actttctatt ataggtcaag
     8341 gtgactctga ttattctaaa ccactttact taacagcagg aggacttttt atacttgtgt
     8401 taatagcaag tattatcatg atatataata gctttaatat gggtgtaata gagagaataa
     8461 agttttttgg actattacgt tgcttgggag catcaaaact ccaagtcaaa aaatttgttg
     8521 ttttagaaag tttaattcta agtgtaaagg ctattccact aggattgtta ttaggatgta
     8581 ttgtaaccat tatatcctct atatttctca aatatgtgaa tacagagtta tttggttcga
     8641 tgccaatttt aaaggttagc tttattggta ttgtgtttgg aataatagta ggatttttaa
     8701 cagtaatatt atcagctatt gttccagcaa aaaaagcttc tggggtgtct ccactaagtg
     8761 ctatacgtgg taatctaatg ttcaaagaaa ttttaactag tggaaaagca agtaaaattg
     8821 ctaacgcagg aagttctaaa attgatgttt ctatgggaat aaatcatgcc ttttcaagaa
     8881 aaaaatcttt tatacttatg acttcttcat ttgctattag tattgtattg tttttaggtt
     8941 ttagtgtatt tatagacttt atgtatcagg cacttaaacc tataaaacca gaagctgctc
     9001 atatatctat tgttgacgct gactttaagt ctgttcttac taaagacgat attaatgaaa
     9061 taagtaatat caaaggaata aaaaaagtat acagtagaat gatgacggat gtagaggcta
     9121 catacaataa taaggaggca acatctcagc taatttctta tgaaaaaaat caatttgact
     9181 gggccaaaaa gtatctagta aaaggggaaa tcaatgaaga taaattagag tcagaaaata
     9241 tcggattggt agaagaaggt catgggttta atattggaga taagatatat attaaaaatg
     9301 gaaattctaa gaaagagata gaggtagcag gtatactttc ttcaatttca tttgatgtta
     9361 aagacaaata tattggaaat attataacat ctgaaaatac ttttactagt ataacagata
     9421 taaaagacta tataataatt gatactcaag ttgatgaaga tgctcctgaa gatttaatcc
     9481 aaaatataag aaatgttcta gatgataatt tagaggttcg agataagcga caaggtaact
     9541 tagaggctaa aaattcattt tacactatgg cagtttttgt atatgggttt gtatttataa
     9601 ttgcgattat aagtatgttt aatatcataa acagtatgaa tataagcgtt acaagtagaa
     9661 taaattacta tggtattatg cgtgcaatag gcatgtccaa taagcaatta agaaaaatgg
     9721 taatagtaga atcaagtaca tatgctgtaa gtggctgttt attggggagt gtacttggat
     9781 taatattaca tagatatata tttgtatcct tagtaacatt aaaattccat atagactggc
     9841 aaataccttt tgatttactg tttatcattg tagtaactat gataataata actttgcttg
     9901 cagtaagaaa accaattaaa aagatttgtg aattagacat aatagaggta gtaaatgcac
     9961 aataaatata agtagtaatt taaatagagg tagtaattta aataaaaaat cgaagtaaat
    10021 atgaaataga aaatatcttt cataatttac ttcgattttt gtgttatata atttacttta
    10081 gataagttcc atcttcatct tgataaatta agtctttctt cataagtact ccaagagcac
    10141 gtttaaaatt ttcttacttg aattaaataa gatagaaata tctttaggat cagatttatc
    10201 attgaatctc atatatccat ctgaaccttc taaatagctt aaaatcttat tttctaaggt
    10261 atcaagttca tcttttctgt ttcctctagg agaaagtcct aatttaccat catcgtatat
    10321 ttttataaca tgtaaatttt caagaacatc cccagctttt aattctgtaa agtattcatt
    10381 atgaaggatt acaccagcat atttattgtc aacacatatc atagcagagt tgttggtttg
    10441 gaatccatat acaactccat ttacaacatc tcctacattg taagtatggt ctgtttctaa
    10501 atatgaatct atgtctgtag tagcagctat tcttccgctt ttatccaagt atatatagaa
    10561 taaatatttt tcatctctaa acaatggata agtcttagct ttaaaaggaa ctagtatgtc
    10621 ttttgtaagt cccatatcta taaaagtacc aatatccgtg ttatcaacta cttttagata
    10681 cgtaacatct ccatttctaa ttatttctat agtattagaa aaataatttt taaatagttt
    10741 gtaaaatatt attgaatttt ttctctcgaa atatataatt tagaatcttc tgatttattt
    10801 ttatgctcga tttccttatt ttttttcata aatatatata gaggagctat taacaacatt
    10861 gcccctaatc ctattaaaac tctattttga gttataggtt ttgaaatatc ttctgcccaa
    10921 gcttgttgta acaaccataa gctacctaaa atagctacca ttggtataac aggaccaaat
    10981 ggaatcttaa aagaggcctt aacatctgtt ctttttctta aaactattac tgataaacaa
    11041 gttggtatgt actgagcaaa tcttgcaatt acactcatta ctgcaagttg ttcaaaactt
    11101 ccagttagca caagtggtat acaacaaatt aaagatatta ttattgctac atatggagca
    11161 ccatatttat ttgtttttcc tataaatgca ggaagagaac cttcttctgc tagagaagaa
    11221 ccacatttag gagttactat tgatgaacct atatttatac caaatataga tattaatgtt
    11281 gctacagata tgaaaaattt accataattg cctaaaaata cacttgaagc atctgcaatt
    11341 ggaatactgt tttcaaatag tctatctcca agtataccca tacagacaat ttgaattaat
    11401 atataaatta ttgcacatat aaatattgtg gttattaatg cgactggaag atttttttta
    11461 ggattttcca tttctccact tgccacaata aatgattcaa agccggtaaa tgcgtaaaat
    11521 actaatataa tggctggacc catactagag ttaactacat ttcctgaagg aacaatattt
    11581 acaaatttta taaagaatat tccaactatt ataaaaacta ttaatggtac taatttagct
    11641 atagttatta cattattcat tatctttgtg cttttaattc caaataagct atttattgaa
    11701 agtagtgtta cgagtattgc agctatataa cctttgtatt cagtggcaga ttctggccaa
    11761 aatgaaccta aagctgttgc aaatcctact gctagagtag accatgatat aattctaata
    11821 acccaagaca ttgtaccaac ttcaaatcct ataaaatctc caaaagcttt tttggagtat
    11881 agatatgctc ctccattctt atcaaacatg cttccaactt ctgcaaaaca taaaagtatt
    11941 gatagaacta agagtgtagc aaaaatgtat ataaacatac tgttttgacc tgctagatta
    12001 taaactttac ctggtagtaa gaatatacct gaacctatta tgctattaat ccctaataat
    12061 atcatactaa atagacctaa tttcttagct ttttccatat taatttatct cctattcttt
    12121 tattaattac tgtactaatt aaaatacaaa gtattcatta catgcatcat aacacctatt
    12181 ggtaaacaat cttcatctat attaaagcta gaggtatgaa gaggtgaaat taagcccttt
    12241 tcctcatttt tacatcctaa atggaagaat gctcctttac aatgttctgt gtaaaaagaa
    12301 aagtcttcac cacctaaaga cgggtttggt ctcaatataa atttttcctc tccaagtaat
    12361 tctttagtat ttgatataac tgtatctact aattcttttt catttataac agcaggatag
    12421 ttttcatcac ttacatgtaa agtcccaaca caaccaaatg cagatgcagt gtctccaaca
    12481 attttagcta ttttatcaat cataaagttt cttgttttgc tatttaaggt tcttaaagtg
    12541 ccttctagtt ttacatcttc acatattaca ttgtgtgcat caccaccata tattttacct
    12601 atagtcaaaa cagcagcatt atttggttct aagtttctac ttattatagt ttgtaatgat
    12661 gtaacaattt gtgaagctgt aactattgca tcaataccat tttcaggata tgcaccatga
    12721 gctcttttac ctttgatact tatttttata gtgtctacac tagcatttaa tgtatcatat
    12781 tttatttcga taaatcctgt ttcgatatgt ggcataacat gaagtccgaa tatataatcg
    12841 accttaggat tttctaaaca tccatcttca actaagaatt ttgctcctcc aaaaccttct
    12901 tctgctggtt gaaataagaa ttttacattt acattaagtt tatcttttat tgaatgtaaa
    12961 actttacatg ctccaagtag catggttgta tgtgcatcat ggccacaagc atgcattttt
    13021 ccagaatgta ttgatttata aggtatgttg ttttcttctt caataggaag agcatccata
    13081 tctgctctta tacaaatcgt tttatctgca ctttctttta aaatatatgc cataattcca
    13141 ttgtgttttg taaattccat ataatcaatt ccgatttctt ttaaataaga aattaccttc
    13201 tgtttagtaa gattttcttc aagagctaat tctggaattt tatgtaattc tcttcttatg
    13261 tttataagcc atggttttat agaattacaa ctttctatta caaaatcttt tcctaattct
    13321 ttggtttgat attttaattt caaagcctgt tgcattttta ttcctccaat tgctttttat
    13381 tgttttatta atataataag caattattat gccataattt tcgatggaga actttgtttt
    13441 ttataagaat attgaataaa tataatcatt tatctatatg tatagtataa aaaatgattg
    13501 catagtttca attttcag
//
LOCUS       DAMJYX010000089        13503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJYX010000089 DAMJYX010000000
VERSION     DAMJYX010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.091"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..342
                     /locus_tag="PC479_002764"
     CDS             <1..342
                     /locus_tag="PC479_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805295.1"
                     /translation="FNNSFLDKKTNKDIVLLVENELRDIVNVEYINQESSLDNHNKKQ
                     VNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEINKLKVNNFDVQNIPNEYKQEKDN
                     IVECKMTVRVI"
     gene            595..1632
                     /locus_tag="PC479_002765"
     CDS             595..1632
                     /locus_tag="PC479_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2805296.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            1713..2078
                     /locus_tag="PC479_002766"
     CDS             1713..2078
                     /locus_tag="PC479_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805297.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2083..2400
                     /locus_tag="PC479_002767"
     CDS             2083..2400
                     /locus_tag="PC479_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805298.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            2448..4052
                     /locus_tag="PC479_002768"
     CDS             2448..4052
                     /locus_tag="PC479_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805299.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4176..5804
                     /locus_tag="PC479_002769"
     CDS             4176..5804
                     /locus_tag="PC479_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2805300.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6069..7418
                     /locus_tag="PC479_002770"
     CDS             6069..7418
                     /locus_tag="PC479_002770"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2805301.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            7574..8653
                     /locus_tag="PC479_002771"
     CDS             7574..8653
                     /locus_tag="PC479_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2805302.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            8998..9906
                     /locus_tag="PC479_002772"
     CDS             8998..9906
                     /locus_tag="PC479_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2805303.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            9911..12280
                     /locus_tag="PC479_002773"
     CDS             9911..12280
                     /locus_tag="PC479_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2805304.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            12605..13408
                     /gene="proC"
                     /locus_tag="PC479_002774"
     CDS             12605..13408
                     /gene="proC"
                     /locus_tag="PC479_002774"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2805305.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT         5475 a         1424 c         2517 g         4087 t
ORIGIN      
        1 tttaataact catttttaga taaaaaaaca aataaagata tagtgttatt agtagaaaat
       61 gaactgagag atattgtgaa tgtagaatat attaatcaag aaagtagttt agataatcac
      121 aataaaaaac aagttaattt agaaataaaa gttaatggag aacttgcaaa tgtgttgaaa
      181 cttgaaggtg taatgaagaa gttaagacta aataataaac ttgaaaagat agaaattaac
      241 aaattaaaag tcaataattt tgatgttcaa aatattccaa atgaatataa acaagaaaaa
      301 gacaatattg ttgaatgtaa aatgacagtt agagtcatat aacaaaaaag aatagataaa
      361 gtaaaaaact attatcaatt aataaaattt tatagaggaa ttagttttac gataattcat
      421 ttctacaaga gggattgttg atgattttat cgaaatccta attaaagtta atatattgta
      481 ccaaaatata agtgaattta atagaatatt tacttatact atagattgat taataaaaaa
      541 atggtataat aacaaattgt actttaattt aataataagg gagtggaagt catattgtta
      601 gggagaaatg agctatgccc ttgtggaagt ggcaaaaaat acaagaaatg ttgtttaaat
      661 aaagatattg tttcagaaag agcatgcaga aaaactgaac tttcacaaaa acaatataca
      721 gatttatatt caaagttata tgattattca agacaggata agtttaaaga agaatgtgaa
      781 aaagctaaag aaatgtttta tataatgcaa aatgatagtg taaatgaaaa atttgaaaga
      841 ttttttaata cttatttaat acaagaccac ataatggaaa ataaaaaggt tatgacagtt
      901 ggttttcttg aagaaaatgg tgcaaaccta agtcaatcag aagtatcaat attaaaaagt
      961 atgtttgaat catacgtaag tatttatgag gtaaaagaag tttctgctgg aaaaatagta
     1021 ttaaaggact gtttaaatgg aaatgaactt tgtacagaag atgtaaaact tctgagaagt
     1081 tttaaaatcg gaagttgtat gatagctaga atagttgata ttgaaggaac taacatattg
     1141 atagatataa ctataagtat atcaaatgag gttaaagata taattttaaa ggatattatg
     1201 aatctattca atcaatatca agatgtatac aaagatatga agtcgttctt aatttatcat
     1261 acacatatac tatacaagta tatacaacag cttttagacc caagtatagc tgaatattta
     1321 aagagagaaa gagataaaaa cgatacaaaa gttaattcag aaaatgaatt agctgaagaa
     1381 gaatgtaaag ttttagatac tcttaaagaa aatgtagaaa aagaaaatat agatgcatgt
     1441 atagagtttt ggaatgaata taagaaaaat catgaaaata taaaaggagc tgaaaatggt
     1501 tgggcagctg ctgttgaata ctatgttaaa aaagaagcag gacaacctat aactcaagtt
     1561 caaatatcaa agaaatacga tataagctct agtacactag gaaagagata caaggattta
     1621 aaaatatctt aattaattat aaaaagcttt aagtcataga taaagaatct atagtataat
     1681 atatgtataa tgtttagaaa gaggtattaa taatgagtct tagtttagga aatgcaattg
     1741 ctgtatcaac gaactttata aaatctaacc cagctgtacc tgtgtacaac ggagaagaaa
     1801 tatatgcagt tatttatcat atagaaatag gatataataa tggtgtatca cctgatacca
     1861 aacaatatat aataatagac tataaagagt ttgatttatc tatggataaa aaaaatgaac
     1921 taataaatat tgctaaagag tactgtaaat ctaaaggtat tgtagaaaac gaaaaccttg
     1981 aaataaacct tttagacaat gaagaagcag agaacttctt agaaaaggtg tttaataata
     2041 tgaaggttat aagaggtctt ataactaaaa aaggataaat aaatggacta tataggaata
     2101 gaaaatataa caccttatga aaatacatat gaatttagtg tatatgaata tgatgatgaa
     2161 atcaccttag gtagtgaaaa gttatatgta tgtgaattaa gggttgtatt gattaaagtt
     2221 aattctctgt atgttgaaag attgcataaa tcagttgaag caatggtctt agtaaaaaac
     2281 ttgaaaaaag atttagataa aacacttgtt gtaaacaaaa taaagaattt tgtgctagat
     2341 gagatttggg tagaaaatct agtaaaagag aatatagaag ttatatttgt agaaagctag
     2401 gaataaccct agctttttta tattacaatc taagagagga aaacaatatg ttaagagtat
     2461 ctaatgtaaa acttggaatt gataaagata tatctttatt aaaagatgtt atacttaaga
     2521 agctaaaaat aaaagaaaaa aacttagtca aatatagtat atataaagaa tctatagatg
     2581 ctagaaaaaa aggaaaaatg gaatttgttt attctgtaga tgtagaagtt aaagatgaaa
     2641 ataaaatact taactcaaaa ataaaagatg taacaaaaat aaaggaaatc agatacgtaa
     2701 atgtacctgt gggtgataaa aaattaaaga ataatccttt ggtaattggt agtggaccag
     2761 ctggcttgtt tgcaggtctg cttttagctc aaatgggtta tgaaccaatc atacttgaaa
     2821 gaggtcttga tgtagacagt agaaccaaag atataagtga tttttggaca actagaaaat
     2881 taaaaaataa ttcaaacgtt caatttggag aaggtggagc aggaactttt tcggatggta
     2941 aacttacaac tagaataaaa gatatacgat gtagaaaagt attagaagag ttagttcagt
     3001 ttggagcacc agatgaaata ttatactcac ataagccaca tgttggaaca gatatattaa
     3061 aaaatgtagt taaaaatata agaaatgaaa taataagttt aggtggtcaa gttagattta
     3121 attcaaaagt aacagatata gttgtatcaa atgattctat acaatctgta ataataaatg
     3181 atactgaaaa aatagataca gatacaatta tattagctgt tggacatagt gcaagagata
     3241 cttatgaaat gttgtattca agaggtataa aaatagttca aaaacctttt gcaataggag
     3301 ctagagttga acatccacaa gatttaataa ataaagcaca gtacaaggat ttttataatc
     3361 atcctaggtt aggagctgca gattatagac ttatagagca tgtttcaaat ggaagaacag
     3421 catatacttt ttgtatgtgt ccaggaggta gtgtcatagc atctgcatca aatgattttg
     3481 aaatagttac aaatggtatg agtgaacatg caagagataa aataaatgca aatagtgcat
     3541 ttttagtaaa tgtaacgcca gaagatttta agagtgataa tccactagca ggtgttcatt
     3601 tccagcagaa gtatgaaaaa ttagcttttg aattaggtgg aaaaaattat aatgcaccag
     3661 ttcagcttgt tggagatttt ataaacaata agacttcttt gaatttagga agtgttgagc
     3721 catcatatag accaggatat acacttacaa atttaagtga atgtttgcct agcttcgtaa
     3781 cagagactat gagagaagcg tttatatctc ttgataaaaa attgaatggg tttgcaatgt
     3841 atgatgctgt tttgacggga gtagaaacta ggtcatcatc accaataaga atagtaagag
     3901 atgaaaatac tttagaatca gtgagtatta agaatctata tccatgtggt gaaggtgctg
     3961 gatatgcagg tggtatagtt acagcagcag ttgatggtat aaaatgtgca gaaaaaataa
     4021 tacaaaaata caccaataat acaaaaattt aacataaact taatgatttt ttaatattta
     4081 acatgaattt aacattatca ctcctataat ttgataaaat acaataggtt ataaaatttt
     4141 tagatacagt aatctcttta caaggaggaa ataatttgaa cattataata agtctaatgg
     4201 gtggattagg tctgttctta tatggtatga accttatggg agaaggtctt caaaaatctg
     4261 ctgggacaaa gctaaaaaag ataataaaac tacttactag taacctattt atgggagttt
     4321 tggtaggtac aggagttaca gcagtaatac aaagttcaag tgctactact gtaatggttg
     4381 ttggtttcgt aaatgcagga ataatgactt taaaacaggc aataggagtt ataatgggtg
     4441 ctaatatagg gactactgtt acagcccaat tggtatcttt cgacttaact ggtatggccc
     4501 cagtagcatt aggtgtaggt attatacttt atttatttgg aaataagcct agaataaaaa
     4561 atatagccga aatacttata ggttttggta tattatttac aggtatggat tttatgaaga
     4621 tggcagtgga gccactaaga gactatcaag gatttactga cttacttgtt acatttggta
     4681 gatatccact acttggatta ttattgggat ttgggattac agcaataata caaagttcaa
     4741 gtgcatctat gggtatgttg gttgcactgg cagcagaagg tctagtgcca ttatcagcag
     4801 cactgccaat tctttatggc caaaatattg gtacatgtgt aacttcactg ttatcaagta
     4861 ttggtgctaa caaaaatgca cgaagagcag ctatgatgca tctaatcttc aatgttttag
     4921 gaacggtgat attcttaata ttcttgaata aaccagtagt aagcatggtt acaagttggg
     4981 acccaagcaa tgttgcaaga caaatagcca atacacatac attgtttaat ataatatctg
     5041 tattgatact tttaccattt acaaatctta ttataaaact agctataaag cttgttcctg
     5101 atagagcagg tgatatagat gaagatgaaa ctaaaactat taaatatata gatgatagaa
     5161 tgatagaaac tccttctata gctcttgcaa atacgataaa agaagcactt cgtatgggtg
     5221 agaaggcgaa ggaaagccta aatgcatcta tggaagcttt agtagaacat tcaactgaaa
     5281 aaatagataa gacatatagg cgagagagat taatcaatga tttacagaaa gctattttaa
     5341 attatttact taaactttca aaagcacctt tagatgatga ttcaagagaa gttgtggata
     5401 ctttgtttaa tacagtcaat gatatagaaa gaattggtga ccacgctaaa aatatagctg
     5461 aattatcgca agttgcaata gattcaaata tttctttctc agaagaagga caatctgaac
     5521 ttgatgttat gtataataga gttgtttcag catatactta tgcattagaa tcaatgagaa
     5581 ctgataatgt tgatttagct tgtaaggtta taaaaattga agaacaagtt gatataatgg
     5641 agaaatcttg tagagctaat cacatgtata gattgaacaa taatctatgt agtatagaaa
     5701 atggagttat ttatttagac gtaataagta atttagagag aatatctgac catgctgtca
     5761 atatagcaca acaagttata gcaaagagat taggaaatga ttaaaaataa ttaatttaaa
     5821 ataatataaa atttaaaata gaggataaat aaaagagaaa ctttaaatga tacgtttaga
     5881 gtttcttttt ttgtaaattt atttgactaa aatttttaaa tttatacata gttatagcat
     5941 ttagaagaat cgatttcctg ataataaatg atgagatatg tatgattttg tggcaaactt
     6001 ttaaaaaaaa cacctatatt ttgtgttata atctctttaa aacgaatatg ctgaaaagag
     6061 ggatttaaat gggaaaaata aattttgatt atagtaaagc tacagagttt ttttgtcaaa
     6121 acgaaataga tgtcatgcaa ccatatgtag atgttgcgca cgacatgtta cataataaaa
     6181 ctggtttagg aaatactttt ttaggatgga tagaccttcc taaaaattat gataaagaag
     6241 agtttgacag aataaaaaaa tctgctgata agataaaatc agactcagat gttcttttgg
     6301 taataggtat aggaggctct tatttaggtt cgagagctgc tatagatatg gtaagtcatt
     6361 cttttaggaa tggacttaaa aaagagcaaa gaaaggctcc agaagtatat tttgtagggc
     6421 ataatataag ctcaacatat attatggatt tattagatat aattgaagga aaggatattt
     6481 ctgtaaatgt aatatctaaa tcaggaacta ctacagaacc agctctggct tttagaatat
     6541 ttaaagatta cttagagaaa aagtatggta aggaagaagc aagaaagaga atttatgcaa
     6601 caacagatgc aagtaaaggt gcacttagac agttagctac agaagaagga tatgagactt
     6661 ttgtaatacc agatgatgta ggtggtcgtt tttctgtatt gacagcagtt gggttacttc
     6721 ctattgctgc tgctggactt gatattgatg ctatgatgaa aggcgcaaat gatgctagag
     6781 aagcatttca aaatcctgac ttaaaaagta atgatagtta tagatatgct gttgctagaa
     6841 caattttaca tagaaaagga aaagatgtag agttattagt gaactatgaa cctcaactac
     6901 attatgtaag tgagtggtgg aaacagcttt atggagaaag tgaaggaaaa gaaaataaag
     6961 gtttattccc tgcttctgta gatttctcaa cagatttaca ttctatgggt caatacatac
     7021 aagatggaaa aaggctttta tttgagacag tacttaatgt tgaaaactgt aagagaaata
     7081 taactataag ttcagaggaa gtagacttag atggtttaaa ctatttagca ggtaagactg
     7141 tagattttgt aaatcataaa gcttttgaag gaactctatt ggctcataca gatggaaaag
     7201 tacctaattt agtaataaat atacctcaac tagatgagta taattttgga tatttagttt
     7261 atttctttga gaaggcttgt ggaataagtg gatatttatt gggagttaat ccatttgacc
     7321 aaccaggagt agaagcttac aagaaaaata tgtttgcttt actaggcaaa ccaggttatg
     7381 aaaaagaaaa ggaagagcta gaaaaaagac ttaaataatt ttaaatagtt aaggtttaat
     7441 taagattaaa ataatataat gaaatcatcg aaaaggagtt tactcaaaat agatgatttc
     7501 atttttttta tgaaaaattg ggaaaaatat attttgataa tttatattat attaagatag
     7561 ggagtggtat gagatgtcaa gaagaaagaa agggataagt ttagtaatat tagttgctat
     7621 aataagttca atacttagta gttttctaac aataatttta gttaaggaca atttagtaag
     7681 taaatctaca ggaagttcta cgccgatagt tgtaaatgat gatggtaaga gtcaaaatat
     7741 ttatcaagca gttgcagaaa aagctacgcc atctgttgta ggtattacaa caactagtgt
     7801 tgatactagt aatatgtttg caataccaac tgaaacacaa ggtgtaggaa ctggaatcat
     7861 agttgattca aatggatata tacttactaa ttctcatgtt atatcagatg gtcaagctac
     7921 aagtgtaaat gtattattca atgatggttc aacaacttca ggtaaagtag tatggtttga
     7981 ccaacaactt gatttagcta tagttaaggt tgataaaact ggactaacac cagcagaatt
     8041 tgctgatagt gacaaagtaa aagttggaga tatatcaata gcaataggta atcctcttgg
     8101 attagatttt caaaaaacag ttactcaagg gataataagt ggattagata gaactataca
     8161 aacagaaaaa actaatatga ctggtttatt acaaactgat gcaagtataa atgcaggaaa
     8221 tagtggagga ccattgttaa atcaaaaagg tcaagtaatt ggtataaaca cagcaaaagc
     8281 ttctcaagca gaaggattag gatttgcaat accaatcaat acagctaaga gtatagttga
     8341 agaagtaatc aaaaatggaa aatatgaaaa agtaacttta ggcataaaag gtacagatgt
     8401 atcaaattat gaagcagcaa caggtacaaa attaagtact gataaaggtg tttatgttgc
     8461 agaagtaata tctggttcat cagcagaaaa agctggagta aaggtagggg atattataac
     8521 taaagtagga gatacagata taacaggtat gaatgattta aataaaaagt tgtatacttt
     8581 ctcaaaaggt gctagtacaa aaataacagt aaatagaggc ggaaaagcag taacaataaa
     8641 tgtgaatttc taaatatgac cagaaagata gagactacat ggtaaagtgt agtctctatt
     8701 tttatttaat ttttatagta tatacatcta aattaattaa aatgatgtat tgtttatagt
     8761 ttattgctat attttcttat aaaaataaat ttaataaata tttaaattta atttacaaaa
     8821 acaatataaa tatcataata aaataaaatt ttttaatatt actaaaaata tatagacaaa
     8881 ataataacga atatagtatt atgtaattgt agaaatgctt ttcataggta tattctatga
     8941 agatgaattg cataaaaatg aaaaaatgga atggatatta tttaggggtg aaaaatcatg
     9001 aatccattag ttataaactt acaaaaatgt agcatacatg atggaccagg gataagaagt
     9061 acagtatttt ttaaagggtg tccacttgaa tgtgtttggt gtcataatcc agagagtcaa
     9121 acatatacaa aacaagtttt gtataatgaa gaaagatgtt caaaatgtga agcatgtata
     9181 aatatatgcc ctcataaggc aatttacaaa ggtgaaacaa aaatatgctt agaccaagat
     9241 aagtgtgagt tttgtgaaac ttgcttagac tattgtgtaa acaatgcaag agagattgtt
     9301 ggtcaagagt attctgtaag agatttggtt aaagagatag agaaggatag aattttctat
     9361 gaagaatcag gtggtggagt aactctatca ggtggagaag taatggctca agatatggac
     9421 ttcatttgtg gagtaataaa catgtgcaaa tcaaagggaa tccatgtagc aattgataca
     9481 tgtggctatg caaagtcaga aaattatgaa agagttgcta aatgtgcaga tttgttttta
     9541 tatgatatta agcttattga tgaagataag cacataaaat ttacaggaaa atcaaatgac
     9601 ttaatactga aaaatgttaa gattttaagt gaattagggg ctaacataaa tataagaata
     9661 ccattaattg taggagtaaa tgtagatgat gaaaatttag aggttaaaaa aatgatagag
     9721 tttttaaagc ctttgaatat acaagcagta agtcttcttc catatcataa tattggtaaa
     9781 cacaagtatg ataaaattta taaaaagtat gaaggagaag aactacaaag accatcagaa
     9841 gaaaaattag aggaaataaa aagactgttt gaagcaagta atttcaatac taagattgga
     9901 gggtaataaa atggcaagag gaacttttga gagaactaaa aaattaagag aagaaagtat
     9961 taatgcagaa ccacacatat ctattgaaag agcagtactt atgacagaag cttataaaaa
    10021 atatgaggga agtgtagaga ttcctgtact tcgtgcttta tcatttaagc attatattga
    10081 gaatagaact cttagcataa atgatggaga attaatagta ggtgaaaaag gtgattctcc
    10141 taatggagca ccaacatatc cagaaatttg ttgtcataca atggaagatt tagaagtaat
    10201 gcacaataga gatataataa attttagcgt atcagaagaa gctagaaaaa tacataaaga
    10261 agaaataatt cctttttgga aaaagagaca aacaagagat aagataataa atgcaatgac
    10321 acctgagtgg ttagcagcat atgaagcagg tatgtttaca gagtttatgg aacaaagagc
    10381 tccaggacat acagtttgtg gagatacaat atataaaaaa ggatttttag atttaaagaa
    10441 agacatagaa gcaagattaa aagagctaga ttttcttaat gatttagatg catataataa
    10501 aaaagcagat ttagaagcta tggcaatagc ttgtgatgca atggttatat taggtaaaag
    10561 atatgctgag aaagcaagac aaatggcaga agaagaaaca gatgaagcta agaaaaaaga
    10621 tttattacta attgctgaaa cttgtgatgt tgtaccagct cacaaaccag aaacttatca
    10681 tcaagctata caaatgtatt ggtttgttca tataggggtt actactgaac ttaatatatg
    10741 ggatgctttt actccaggaa gacttgacca acatctaaat cctttctatg aaagagatgt
    10801 agaaaatgga atactagata gagatagagc acaagaatta cttgaatgtt tatgggttaa
    10861 atttaacaat cagccagcac caccaaaagt tggtataaca ttaaaagaaa gtagcacata
    10921 tacagatttt gcaaatataa acactggtgg aataaatcca gatggtcaag atggtgttaa
    10981 tgaagttagt tatataatac ttgatgttat ggatgaaatg aaattgatac aacctagttc
    11041 aaatgttcaa ataagtaaaa agactcctca aaaattctta aagagagctt gtgaaatatc
    11101 aagaaaagga tggggtcaac cagcatttta taatactgaa gcaatagttc aagagcttat
    11161 ggaagctgga aaaactatag aagatgcaag acttggtgga accagtggtt gtgttgaaac
    11221 tggatgtttt ggtaaagaag catatgttct aactggatat atgaacattc caaaaatact
    11281 tgaattaaca ttaaataatg gatatgaccc aattagtaaa aaacaaatag gtattgaaac
    11341 tggagaccct agaaatttcc aatcttatga ggaactattt gaagcattta aaaagcaatt
    11401 acattatatg attgacataa aaatagaagg aaatgctgtg atagaaaata tatgtgcaaa
    11461 acacatgcct tgtccactta tgtctacaat agttgatgat tgtatagaaa aaggcaagga
    11521 ttaccaaaga ggtggagcaa gatacaacac tagatatata caaggtgttg gtataggaac
    11581 tataacagat agtttaacag caataaaata taatgtattt gataagaaaa aattcgatat
    11641 ggatacattg cttaaagcac ttgatgctaa ttttgaagga tatgaagcta tacttaattt
    11701 agtttcaaat aagactccta aatatggaaa tgatgatgat tatgctgatg aaataatgca
    11761 agagatattc aatgcatatt acaatgaggt aactggtaga ccaactgttt gtggtggaga
    11821 atacagagta gatatgttac caacaacttg tcatatatac tttggagaaa ttatgggagc
    11881 aagtccaaat ggtagacttt gtgcaaaacc agtttcagag ggaatttcac ctgaaaaagg
    11941 tggagatact aatggaccta cagcagttat aaaatcttgt gctaagatgg accacataaa
    12001 aactggtgga acattattaa atcaaagatt tgcaccatca gtagttcaag gagaaaaagg
    12061 gttagataat atggctaatc tagtaagagc atactttaat atggatggtc atcatataca
    12121 atttaatgta tttgataaaa atgtactgtt agaggctcaa aagaatcctc aggattataa
    12181 agacttaata gttagagttg caggatatag tgaccatttc aataatttaa gtagaacact
    12241 acaagatgag ataataggaa gaactgagca aacattctag gtttgcttgt aaataaaact
    12301 tcttactata taaaatcgta actgatataa taaagtataa atggtttgat tccattttta
    12361 aaattttaga ttaattatga gctaactttc taaataggaa gttagctcgt taagtttaat
    12421 gaaaaaatcg ttaaatttat atttagtaaa tcatacacat attagtaagt aatacataca
    12481 aaagaataat ttattatagt tataataagg ttatatttta taaaaataag ataattttaa
    12541 gcagttaaaa taaagtcaat ttagctactt taaaataaaa ctaatagaaa aaaggagatt
    12601 tgatatgaaa actttaggat ttattggttc agggaatatg ggaagtgcaa tgataggtgg
    12661 catagtacag tcaggattag taaaggcaga agatataact gtatcagatt taagccaagt
    12721 tgccttagat aaagtaaaag aaaaatttgg agtaaatact acaactgatt caaaggtagc
    12781 tgttacaaat tctgatgtgg taatagtagc acttaaacct aatatatgcg aaaaggcttt
    12841 aactccactt aaagaattaa tagatgcaaa taaaataata gtatctatag cagcaggtaa
    12901 aactatagaa agtctggaaa gttttatagg ttcagataaa aaaatagtaa gagtaatgcc
    12961 taatactcca gcccttgttg gcgaaggaat gtcagctctt tgtaaaaata gtaatgtaac
    13021 agatgaagag ttaaatatga ttaaggctct gtttgaatct tttggtgagg cagaaatagt
    13081 atcagaatat ttaatggata cagttacagg tattagtggt tcttctcctg catatgtatt
    13141 tatgtttata gaagctatgg cagatgcagc agtattagct ggaatgccaa gacaacaagc
    13201 ttataaattt gcatctcaag cagtaatggg gtcagctaag atggttcttg aaactggaaa
    13261 acatccaggt gaattaaaag atatggtttg ttctccagca ggaactacta tagaagctgt
    13321 taaggtatta gaagaagaag gctttagagc agcagttata aaatctattg ttgcttgtat
    13381 agataagtct aaagatatga gcaaataaca gatgtaatta agtttactat atggtttctt
    13441 gaattgtttt tttagtgctt atttatatct atacataaat gtatttggta taagtaaaat
    13501 aag
//
LOCUS       DAMJYX010000090        13371 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMJYX010000090 DAMJYX010000000
VERSION     DAMJYX010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.092"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            201..1277
                     /locus_tag="PC479_002775"
     CDS             201..1277
                     /locus_tag="PC479_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2805306.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1380..2222)
                     /locus_tag="PC479_002776"
     CDS             complement(1380..2222)
                     /locus_tag="PC479_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2805307.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2406..2978
                     /locus_tag="PC479_002777"
     CDS             2406..2978
                     /locus_tag="PC479_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2805308.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3141..3413)
                     /locus_tag="PC479_002778"
     CDS             complement(3141..3413)
                     /locus_tag="PC479_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805309.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3540..4412)
                     /locus_tag="PC479_002779"
     CDS             complement(3540..4412)
                     /locus_tag="PC479_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2805310.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4708..7815)
                     /locus_tag="PC479_002780"
     CDS             complement(4708..7815)
                     /locus_tag="PC479_002780"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2805311.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7908..8140)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8315..8842)
                     /locus_tag="PC479_002781"
     CDS             complement(8315..8842)
                     /locus_tag="PC479_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2805312.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8877..9662)
                     /locus_tag="PC479_002782"
     CDS             complement(8877..9662)
                     /locus_tag="PC479_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2805313.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9672..9929)
                     /locus_tag="PC479_002783"
     CDS             complement(9672..9929)
                     /locus_tag="PC479_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2805314.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9948..10406)
                     /locus_tag="PC479_002784"
     CDS             complement(9948..10406)
                     /locus_tag="PC479_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2805315.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10446..11150)
                     /locus_tag="PC479_002785"
     CDS             complement(10446..11150)
                     /locus_tag="PC479_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2805316.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11160..11795)
                     /locus_tag="PC479_002786"
     CDS             complement(11160..11795)
                     /locus_tag="PC479_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805317.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12176..13216
                     /locus_tag="PC479_002787"
     CDS             12176..13216
                     /locus_tag="PC479_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2805318.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4270 a         2263 c         1573 g         5265 t
ORIGIN      
        1 agacttaatt tattactttt atagaatttt cacaataaaa agtcatttta cttccgtatt
       61 taatgcaaaa gtagaaatta aacttaatat aaaaaatata taacaaattt tttttatatt
      121 tctagaagaa aagtatactc ttttttgttt aataatgcta aaattgattt gtattgaatt
      181 ttgattagga ggaaattatt atgaaagctc aaagattaaa tgctgtactt gaacaaatga
      241 aaaaagatga tatttctcaa atgttagtaa gtgaccctac ttctatattt tatcttacag
      301 gagtactaat ccatccaggc gaaagactat tagcattata cttaaattta aatggcaata
      361 ataaattatt tataaatgaa ttattcccag tttcagaaga tttaggtgtt gaaatggttt
      421 ggtttaatga tactcaaaat ccagtagaaa taataactga acatatagac aaaaatgcta
      481 ctatgggtgt tgataaaaac tggccagcta gattcttatt aaacttaata gaattaggtg
      541 gaggaagtaa gtttgtaaat tcatcttata taatagatac tttaagaatg tgtaaagatg
      601 aagaagaaaa agagttaatg agaatagctt caaaattaaa tgacaaggct atggaacaac
      661 ttaaagctac tgtttctggt gaacttacag aaaaacaact tgttggtaaa ttatctaaaa
      721 tatatgaaga cctaggtact gatggatttt catttgaccc tattataggt tttggaccta
      781 atggagctaa tccacatggt gaacctggaa atgcacttgt gaaaccagga gatgcaataa
      841 tcttagatat aggttgtata aaagataact attgtgctga tatgacaaga actgtattct
      901 ataaagaaat acctgaaaaa ggtagagaaa tatttgaaat agttcttgaa gctaataaga
      961 gagctgaagc tattgtaaaa ccaggtgtta gattctgtga tatagatgct gctgctcgtg
     1021 actacataac tgaaaaggga tatggacagt acttcactca tagaactggt cactctatag
     1081 gacttgaagt tcatgataaa ggagatgtat cttctataaa tacagatact gtacaaccag
     1141 gaatgatatt ctctatagag ccaggtatat atttaccagg tgagttcgga gttagaatag
     1201 aagatttagt acttgttact gaagatggtt gtgagatatt aaacaaacat gacaaagaaa
     1261 tatgtgtagt tggttaattc taaaatcaat ttgataaaat taaaaagcca tataaaaatg
     1321 ttttaaacat ttttatatgg ctttaatttc tcaatctatg atttatttca atccattatt
     1381 tatattgcag ttattaagat tgcacccttt gaagtatatg tccccatcac tgaaccaatt
     1441 tcacagatta caacatcttt gtattttaca tccttttcca taacttccct taccttttga
     1501 gccttttctg ggcaattagc atgtcctata aataaaacct tttcttcttt atctttaatt
     1561 tttgactcta ctaaagtaat cagctttttc aaactgtttc cttcacctct tgccttatct
     1621 ataggagtta caacgccatc tgcaacttgt attatcgcct taaaatttaa ggtatttata
     1681 atttttcctg ctattggatt tattctacca cccttaatag cattttctaa agtttcaagt
     1741 gttccaaaaa atggaggatt atctttatat ttatttatta ttgaaacaat ctcttcaatt
     1801 tcttttcctt cttttattaa tattgctgct tttaacacta attgtcctat tccaattgac
     1861 ccactaagtg aatctataac ctcaactttc ttgttatttc cttccttttc aaacatatct
     1921 tttgctaaaa gtgcactatt ataagtccca gataatcctg aacttatatt aatcactaat
     1981 acactttctt cctgacaatg gtatgcttct aaaaatttat caggagaagg acatgatgtt
     2041 tttggtagta ctttactctc actcattttt ctatagaaag tttcattatc tatttccttt
     2101 ccagatatat aactttcttc tccaaaagat acatttaaac caacaacttc tatgttgtat
     2161 ttttctatta tatcatcagg taagtcacat gcactgtcca ctatcaattt tattttatcc
     2221 atattaacct cactataaga tattatttta tttattcagt cggtcgtacg accgatttaa
     2281 ttattatttt atatattgaa tgaaaaaaag tcaatagata tagattctcg ctttattgac
     2341 aataaatatc cataagctta taatgttatt aatttgttat aaatatacat ggaaaggtag
     2401 gttttatgaa aacaaataat atgaaacagg ctattttatc atctgcaacc aatttaatag
     2461 ccaaaaatgg agttcaaaat actagccttg ctgatattgc aaaagatgta aatataagta
     2521 aaggtacttt gtattatcac tacgcatcta aagatgatat tatatatgat attgcggata
     2581 ctcatttaga agttataact agtgctatac taaactgtgt aaaaaatgtc aaatcaaaaa
     2641 attctcaaat agaaatggtt aacttaatct tagagaaaat atctactata gaaagtcgtg
     2701 gtagggtaca tatgtatctt atttgtgagg caattacttc aaataatgat ttaaaggaaa
     2761 gaataagatt aaaatatatt gaatggagaa ctactcttaa atctgaaatt gttgaatcac
     2821 tagaaaaata taatagtagc gaaaatgaac aggatgctga atcattttct tttttactca
     2881 tgtcaatagt tgatggttta gttgtccaaa gtttattaaa aacagaaaaa ataccttatg
     2941 aaaatatagc ttctttttta gttaatcatt ggatttaaac ttaatactta gataattaat
     3001 aaaagtgaac tatttaagtt atctacttta acagttcact ttcttattta ttctcaataa
     3061 tttttactta aaacattcaa tttttattta aaccttcatg gaatcacaaa ataaaaatct
     3121 cttataaatt taaaatgttt ttatttatgt tcatgtgtat gcccatctga atgttcatga
     3181 ccatgtgtat gtgtatgctc atggctatgc tcatgagtac ctgaaaaacc atcatcatga
     3241 ttatggtcgt gacctacacc atttacatgc tcatgttcat gcccatgact gtgagaatgt
     3301 tgatgtgaat gttcatggtt atctcctcca cagcatcctt ctcctccagt cccacaacac
     3361 ccacttgtat gtggattatg atgatttctt actacaaatc tctctcttaa cataattctt
     3421 atacctccta gtatattatt gagtacttca tgtaccccat taactacagt atacatctaa
     3481 ctaggtataa tctcaataca tatggcattt tttaacatat ataattaaca tatataatct
     3541 taaagaaatt tatccttaaa acctccacct tttacttctt caacgttatt gacagaaata
     3601 aaagcttcac tatccatatc ataaattaaa tctttaaatt tacctatttc atttgcagat
     3661 attatgcaat aaatcatctt tttcttattt tgtgtatatg caccttctgc ctccaaaaaa
     3721 gtgactcctc tacccatttt attcattatc actttggata tttcatcata tttatttgat
     3781 attaaaagta tagatttttt tctatcaaag caatcttttc ctatatccat agtaaaagaa
     3841 attatataca ttgtaattaa agtgtacatc accaatttag ctccaaataa aaaagctcct
     3901 gcacatacta caaaaaaatt aataaataaa aatgtatttt tcataggtat atcatacttt
     3961 cttttcatta tggctgctat tatatcaagt cctccagcag tagctcttgc tttaaaagtt
     4021 aaccccatac ctataccagt taatgcacca ccataaatac actgtagcat agtatcatct
     4081 attgggaaat atttagcaat atcttgtgtt agccccagtg ctagagaaaa tagtaacata
     4141 ttgacaaaac ttattagcaa aaatttatta tcaaaatact ttctagctat aataaatatt
     4201 ggtatgttca tcaagaaact tgatattccc tgattaatac caaataattt gtataatata
     4261 acacaaattc cagctactcc tccacttaat aacttggctg gtgctagata taaatttatt
     4321 cctaaggttg atagaatcat acccaatata ataagacaat attgtaaaaa tatattatta
     4381 tcaatactta gctttttact ttcaccatac atcaaaacgc acccctttgc ataataaata
     4441 aaccaccctg cataacacac gataaattta caaataaatt tatcagaagt agtttcctaa
     4501 aaatattatt catcctcact taaacgttta ttcattaata ttctatcaca tgacataaat
     4561 aatttatatg atttatatag atttatttat tgtaaatttt ggaatattaa aacattttat
     4621 tgaatagata attatattac ataaaaaatc cctatagaaa tctataaact aatttctata
     4681 gggattttgg ttaactaact attattttta tttttttata acttttatac ctgtcatatg
     4741 gtcatttaag ttttgttcct ctaaagattt atcatctact ctgtttattt ctaaagctaa
     4801 agtttcagac ttgatatatt cattaaaatg gtcaacagcc ttagcaattt catcatcacc
     4861 acagtattct attactatat tatctaatac ttcaaagtta ttagattttc ttaattgctg
     4921 tactttagat ataaattctc tagcataacc ttcattaatt aactcttcag ttagcttagt
     4981 atctagtata acaaaaagat tgttttcaac agatacatta aatccttcct tagcagttat
     5041 acctattaat actaaatcct tattaaattc aaaagcttca ccatctaaat caactgttaa
     5101 agtctcacct ttttcaagtt tattagcagt ttcatgagca tttaatttac ttaatgctcc
     5161 aacgaataaa ttcatcttag aacctaataa tggccctgct tccttaaagt ttggttttaa
     5221 actaaagttc atatactcat ctaaatcctt agcaaatatt acctctttaa cattaagctc
     5281 ttcttttatt aaatcaacta catcacttat tgtatcttca aacttaccat ctactaacac
     5341 tttttgtaat ggttgacgaa cttttattct ttctacttct cttgaagctc tacctaaagt
     5401 aactaagttt ttagctaaat ccattttttc ttctaattta gtatctacta actcttcatt
     5461 acatttagga tattcagcta aatgaacaga tacttctcct gttaagtttc tatacatttc
     5521 ttctgacata tatggtgcaa atggagctat agctctacaa agaccatgta acaattcata
     5581 agttgtattg tacactgctt ttttatcttc tgttaattca gttgcccaga atcttcttct
     5641 tgaacgtctt atataccagt tagataaatc atcatttata aaatcttgta tcattcttaa
     5701 agttttattt aactcaaata tagctaaatt ttcttctaca tctttcatta aattattgaa
     5761 cttagataat atccatctat caagttctgg tctatcttta tattctacaa aaaattcagt
     5821 tggatttata tcatctgtgt ttgcatataa agtaaagaag ttatatacat tcttcatagt
     5881 acctataaat ttactttgga tttcttttaa tccatccata tcaaatttag ttggagtcca
     5941 tggtggagaa acatatagta aataccatct taatgcatct gctccatatt ggtcaaataa
     6001 ttccattgga tttactgtat ttcctcttga tttactcatt ttcttaccat ctttatcaag
     6061 tacaagatct ggaactaaaa cactcttata tggtgctttt cctaatacaa atgttgatac
     6121 tgcaagtaat gagtagaacc aacctctagt ctggtctata ccttctgata tatagtcagc
     6181 tgggaacaat tggtcaaagt tttccttatt ttcaaatgga tagtggtgtt gagcaaatgg
     6241 catagcccca ctatcaaacc aacagtctat aacctctgtt actcttgtca ttggttttcc
     6301 acatttttca cattttaagt gtatatcatc tacatatggt ctatgaagtt caactgtttc
     6361 tggatttaca tcttctatag ctttttcagc taattcagct cttgaaccaa ctgaatctgt
     6421 atgaccacat tcacatctcc atataggaag tggagttccc caatatctag ttcttgatat
     6481 tgcccagtca tttagatttt ctaaccagtt tccaaatctc ttctctccta caaaacttgg
     6541 gaaccatttt acaccattat tattttctat taatttatct ttaagtttag tcatctctat
     6601 ataccagctt ggttttgcat agtatactaa tggagtttga catctccagc agtgtggata
     6661 attatgaact actttttctt tgctatataa tttattttca gaagctagcc attttattat
     6721 ttcaacatct agcccatctt ccattacaaa tctaccttcc caaggagtag atgtaaattt
     6781 accagtttca tctactggtt gaataactgg taaatcatat cttttacctg tgttgtagtc
     6841 atcttcacca aatgctggtg ctgtatgaac tataccagtt ccatcttcag tagttacata
     6901 atctcctact gtaataaaaa atgctttttt gtcaacttct acaaaaggca ttaattgttc
     6961 atattctaaa ccttctaggt ctttaccttt catttcttct aaaacttcat aatcttcacc
     7021 tagtacttta tcagctaaag gtttagatac ataatatacc tcatcacctt ttttaacttt
     7081 taaataatct acatcagcat ttactgttaa tgaaacgttt gatggtaaag tccatggagt
     7141 tgttgtccat gctaagaagt attcatcagc atcttttttc ttaaatttag ctataactgt
     7201 attagttttt acttctttat atccttgtgc aacctcatga ctagcaagac cagttccaca
     7261 tcttgggcaa taaggaagta ttttatgtcc ttcatatata tatccttctt tgttgaattt
     7321 gtttagtatc caccatacag actctatata gttattatct aaagttatat atggattatc
     7381 taaatcaact tcataagcca ttctctcagt cattattctc cattgttttt caaatgagaa
     7441 aactgactct ctacattttt ggttaaattc agctattcca tattcttcta tttgttgctt
     7501 gctagtaaga cctaactctt tctctacttg tatttcaact ggtaatccat gtgtatccca
     7561 accagctttt ctctttactt gataaccaga cattgtttta tatctacaaa ctgaatcttt
     7621 aagcgttcta gataaaacat ggtgtactcc aggatttcca ttagctgttg gaggtccttc
     7681 atagaatacg aaacttgggt catctttccc tttttctaaa gttttttcaa gtatatttat
     7741 gtctttccaa taatcaaaaa cttgtgcttc tgcttgtttt acagaactgt ctaccaatgg
     7801 cttaaatttc gccatgtaaa tcaccctttc ctactaaata ttataattcc aaaatttttt
     7861 attctttcac ataaatttaa atactaaaaa actcgccccc tttaaaccaa agggacgagt
     7921 tataaatcgc gttaccaccc taattctata tgtaaactaa atactagcta catataacac
     7981 tctaatagta taacggtact aaccggcaca gcttagtaga atttaatcgt tcagcctgca
     8041 gctcaagagt gatttttatc tttataaatt cattggttct cagcataccc aattctctgt
     8101 aaaattatct aaagataaat gtctctatca acgcttttaa aaatttatct gtgtttatat
     8161 gaaattctat aacaaataat ttgaatagtc aatatatttt tgaaaaatat aataatttct
     8221 atttttctta catttttatc ctatttttaa tctttgttat agatttctca aactcacaca
     8281 attattctac ataaaaaata aacaaattac aagcttattc taaagttgta gcagcttcat
     8341 cattactata attatacact gttgttcctt caaatgcatt tacacatttt tcatctacat
     8401 cataaaaaat ctcatcaaca cttcttattt catcttctaa cagtgattta aatttatttc
     8461 taaatatctt aaattctttg actaaagcat catactcttt tcttatctct ataacacggt
     8521 tattagcttg ttctataatt tgtctagctt ttaaatcagc ttcttcaaca attatcttag
     8581 cctttttatt agccgcgcta caagtgtctt ctgctgcact ttgagcagtt attaaagtag
     8641 cttttagtgt ttcttcaata ttttcatact tgtttacttg gtcttgatat aaccctaatt
     8701 tttctttaag agctgtattt tccctacata gagattcata atcttccttg atgatatcta
     8761 aaaattcatc aacctcttca tctctatacc ctcttaaacc ttttttaaac tctttatttt
     8821 ctatctcaat tggagttagc attacatttc ctcccagcta taatttaaat tagtccttat
     8881 acaattaatc ttgcttgaac ttttattttt ccttttttag taatatctcc tatttgtgtt
     8941 accttagctc ttcctttgcc tctaatagaa atcaaatcat cgttttttac tattttagaa
     9001 gttgaagtta tcttttcata attgacatgg actttttctc catttatgta ttttgcacta
     9061 tcttgtctag atatattgta aataccactt atcacacaat ctaacctatc tgaagatact
     9121 gtaaatgata cttctttata ttttgaagaa ctattaacaa tattttctct ggaaatttcc
     9181 tttacaatta cattatttct tgaaaccttt tctaagttta caattataaa atcgcaaata
     9241 tcactactta caactacttg acaaaagcta tcatgtataa ttatatctcc aattttttct
     9301 ctttttatac ctagacccag tattgaccct aagtaatctt tatgagatat atttttaaat
     9361 ttgaaattcc cttctatttg taaaaatcta agagtatctt ctatatcttc atattccatg
     9421 taaaatggat aaataaatac agtgcttctt tcagcttgtt cataacctcc atccacactg
     9481 tattttattt catttgtaga atttaatatt gctacagcat ttttcacttc atatggatta
     9541 agaaactccg tagacttaac atcatagttt tttatacaac tattagcctt gtctatcact
     9601 ttaaacattt tatttttcaa gtctatatcc tttatgtgat ttgttagttt taatttatcc
     9661 atcctatcac cttagaatat aaccaatact aattgtctta ggaacatcaa taatactatt
     9721 gcaattaaag gagaaaaatc tataggtcca tttgtatatt tatacattat agaccttatt
     9781 ggcccttcta ttggctctgt aagattttcc aaaatatcaa aaattttact ttgtcttcca
     9841 tttggaaacc aagtcatcaa gctttttata actatcatcc atgctattat atctaaaaga
     9901 taataaagtg caattcttat tgttcccata tattaaccgc ctttttacta tttattttgc
     9961 caagggaaga atgctttact ttctaattct tttttcatat ttgcatctat atccacatta
    10021 ttaggagcta gtataaatat tgatttagaa accttttgta tacttccatc taagacataa
    10081 actgccccat tcataaagtt aaatatagat ttacgaactt cagaattagt caatccttcc
    10141 aaattcacta taactgccct tctttgtttt agatggtctg ctatagttgc agcttcatca
    10201 taaaccttag gttcaactat tacaactttc atttggctag ttgtatgtaa atttacaatt
    10261 ttatttgctt ttgcagtact aaagccactg ttcatttctt cttcttgaac tatatcgtcc
    10321 atgccacttt catattcatc ttcaatgtaa tcttcttctt catcaactat ccaattctta
    10381 aatttagata ttattccatt tgacattatt aacttcctcc ttaaaattta ttcaaatttg
    10441 agtaattatt ttttattgta gtttctttct ccaaagattg atgttccaac tctaacaatg
    10501 gtagcccctt cctctatagc aatttcataa tcgtgactca ttcccattga caaaacatcc
    10561 atccctacat tagggatgtt tagttctttt atttgttttg ataaatcttt aagaccttta
    10621 aatacacctc ttatctcatc ttcatcttct ataaaaggtg ccattgtcat tagacccttt
    10681 atttttatat ttttatattt ttctgacact tctttgacaa agtctacaac ttcttctcta
    10741 tcaagtccat gcttagactc ttctttagat atattcactt gtactaagca atctatttct
    10801 ctgtttattt gttctgcacg tttttgaatt tcttcgcata atgcaattct atctaaagag
    10861 tgtatcatac aaactttatc gattatgtac ttaactttat tggtttgcaa agttcctatc
    10921 aggtgccagt ttaatttatc acctataata tcatactttc tagctaattc ttggggttta
    10981 ttttcaccaa cagaagtgat tccttccctc attgcctcat taactaagtc tacatcaaca
    11041 gtctttgtga ctgccaaaag ggtaatctct ttgctgtctc tatctacttt ttttgcagct
    11101 ttatcaattt tgtttcttat ctcagacaaa ttatctttaa tagaacccat tattatcacc
    11161 tactttatcc ttatatttgt gtttatactg ttaggtctta aaattatttt gtcatataga
    11221 ttaacacttg taactccatt tatgtcattt ttataataat ccacaaatac agaagattca
    11281 ttctcataag ttattccttc taatatgaca aattttacac tttgtgattg ttcatcaatt
    11341 acatatacac cacgtttatt gtcttttgtt tttatagaag ttttaggtat ttttaaacat
    11401 tccatctgat tatatattat atcaaattct tcgacacttg tatcctgatt tgcaacaatt
    11461 tggttactta tttttaattt taccattatt ttattattat tttcatatat tttgtaaact
    11521 tttgcatcta gtttttgctc atgatattca attattacat tttgattttt ttcaaagtta
    11581 ttagaatctt ctttactgat gtatgtagat agataattac tttggtcatt ggcatctttt
    11641 tctatagttc cttttacatg aatctttgca tcaacttttt cattttttaa caccaacgta
    11701 tctacatttt tgctaattat cattactaat atattaaata ctaaatatat aagaatacca
    11761 agtatcacta atctggtata ttttaccttt ttcaaagact ctccccctat aaaattactt
    11821 tcattataag tacttcttct aaaaaatgtt tattcctttt ttataagata atattttacg
    11881 ttctaataat tattttttcc atattaaatt tattttttat caatcttcct atacaagact
    11941 gcattattta tatataattg tattataaag tataagttta atttacattt tatacgttaa
    12001 gcttacttta tatattttta tagttatttt tatttttaga tgcaatataa aaccaattaa
    12061 gatttaaaat aacttttgtt atgttgacat ttatttaggt aatcatttat gaattaggat
    12121 aacgttatca caacaaaagt ttatgtccta aaaaaaatac aaggaggatt ttcttatgtc
    12181 agaaatttta ttaggaacag gtttactttt attggttcta tgttgtttca caatttttag
    12241 ttacaaggcc ccatatggta tgaaggctat gggagctcta gcaaacgctg catgtgctac
    12301 tttcttagta gaggcattca acctttcttt acttggtgat gttttaggta tggaattttt
    12361 tagaagtgtt ggagctgcta atggtagtct tggtggagtt gctgctgcta tacttgtgcc
    12421 tttagcctta ggtgtatctc cagtttatgc agttttaata ggattatctt gtttagattt
    12481 ttctatatta tctggattcg ttgctggata ctttgtttct ttcttaatta agtttataga
    12541 gaaaaaagtg ccacctggag tggatttagt ggttgtaatt cttctatgtg caccactatc
    12601 tcgtcttata gcatcaaatg tagacccatt agttaatagt acactactac aaattgggca
    12661 agttttgata gaagctacca acattagccc tataataatg ggaattatac taggaggtct
    12721 aataacagta gttggaacgt ctccacttag ttcaatggct cttacatcta taattggtct
    12781 tactggtctt cctatggcaa taggagcatt aggtgctttt ggttcagctt ttatgaactc
    12841 aacactattt gcaaaattga aattaggttc taaaaaagat attatatctg ttgctattga
    12901 accattaacc caatcagaca taatttcagc taatccaata cctatatatt ctactaattt
    12961 ctttggagga gccttttctg gtatcattgt agccttgatg ggtcttgtaa acatgacacc
    13021 aggtacagct acacctgtgg ctggttttgc tataatgttt gcttataatc cagttcaaca
    13081 agttcttata acagctggtt tatgtgcttt attcaatgta attgcaggat ttttaggttc
    13141 aaaaatattc agtaccttta aagttcttac tgctggtgaa atacgtggta ctgatgtaga
    13201 agaagaaatt gcttaaaaaa caataagatt tataaattta tatctcaagt agtagagttt
    13261 tatattgtag gcaattttat taattgcaaa cgttttattt tttatttatg tttatgatta
    13321 ttatgtgtgt tgaatattta gataaatttt tatttattat ttttatttag a
//
LOCUS       DAMJYX010000091        13088 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJYX010000091 DAMJYX010000000
VERSION     DAMJYX010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.093"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            131..1504
                     /locus_tag="PC479_002788"
     CDS             131..1504
                     /locus_tag="PC479_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805319.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1760..2827
                     /locus_tag="PC479_002789"
     CDS             1760..2827
                     /locus_tag="PC479_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2805320.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2971..3279
                     /locus_tag="PC479_002790"
     CDS             2971..3279
                     /locus_tag="PC479_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2805321.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3272..4702
                     /locus_tag="PC479_002791"
     CDS             3272..4702
                     /locus_tag="PC479_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2805322.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4780..5154
                     /locus_tag="PC479_002792"
     CDS             4780..5154
                     /locus_tag="PC479_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2805323.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5148..7352
                     /locus_tag="PC479_002793"
     CDS             5148..7352
                     /locus_tag="PC479_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2805324.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7352..8761
                     /locus_tag="PC479_002794"
     CDS             7352..8761
                     /locus_tag="PC479_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2805325.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8746..9810
                     /locus_tag="PC479_002795"
     CDS             8746..9810
                     /locus_tag="PC479_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2805326.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9840..11261
                     /gene="nhaC"
                     /locus_tag="PC479_002796"
     CDS             9840..11261
                     /gene="nhaC"
                     /locus_tag="PC479_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2805327.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11690..12154
                     /locus_tag="PC479_002797"
     CDS             11690..12154
                     /locus_tag="PC479_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2805328.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12388..12750
                     /locus_tag="PC479_002798"
     CDS             12388..12750
                     /locus_tag="PC479_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2805329.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
BASE COUNT         5163 a         1432 c         2534 g         3959 t
ORIGIN      
        1 aatgtacata aaaaatatca taaatatatg ataagataaa tacaatagca acattttatt
       61 aattttttgg gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt
      121 ttttaaaata atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa
      181 agacgggaaa actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca
      241 cgatatttta accaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac
      301 actattagaa gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga
      361 taaaaatgga agagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga
      421 gtttgtaggg gcacttgaga tatcaaagaa tatgacaacg atagaaaatt tactaaacag
      481 aaattataaa aaaaatttag tcaataaagt tagtattaga caaaaaaagg gttatgaatt
      541 tgatgatatt ttaggggaaa gtaatttgat taaagaagca atagctaaat ctaagaaagc
      601 ttgcaagtct gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt
      661 tagccagtcc attcactatg gaagtaatag gaaaaactat ccatttatag cacaaaattg
      721 tgcagctttg ccggaatcac tattcgaagg tattcttttt ggaactagca agggaggatt
      781 tacaggagca atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt
      841 ggatgagata aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga
      901 aggttatgta agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc
      961 aacgacaaat gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata
     1021 ttataggctt aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat
     1081 acctctattg tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtagt
     1141 aggaatttca gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag
     1201 agagctagaa aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga
     1261 aacaagtatg attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa
     1321 ttattcttta aacgatttgg aggacaaaac acttagtaag attatcagtg agatagaaga
     1381 aaagtatata tatgaatcat tagaaaaggc atcttacaat ataactaaag ctgcatcaat
     1441 tttaggtatg aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt
     1501 ataaataagc aagataaaaa aacaaaatca aagtaaaata aaaaagcaaa atagaggagc
     1561 aaaattatta taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt
     1621 attttttatt tatgaaaata cataataaaa taattatagt ttcaacgttt caataaatat
     1681 ttactttaat tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa
     1741 tttttaggag ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat
     1801 gggaataggt atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg
     1861 tagtagaagt acttcaggaa aaagtatgta taatgtttta ggaattgaaa gaggagaaag
     1921 accagaagtt attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt
     1981 tgttttgtta gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt
     2041 aaatagaaaa ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga
     2101 tgcagattta gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg
     2161 aacaggaata aatccaggat ttgtattaga tttactagta ttggcgcttt caggcacttg
     2221 tgaagaagtt actagtataa aagctaagag agtaaatgat ttatctccat ttggaaagtc
     2281 tgttatggag gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga
     2341 taagactata gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt
     2401 aggttggaaa ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag
     2461 aaaatctaag tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata
     2521 tggatatgta aatggagaag ttttaattga aatggagcat ccacaacaga taataccaga
     2581 agcagaaggt attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt
     2641 acaaataaat cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat
     2701 tccattaata ataaatgcta gaccaggtct taaaactatg ttggatatac cagtacctag
     2761 agcaataatg ggagatataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc
     2821 taattagtaa gataacagct aactaattag taagatagca actagctaat tagtaagata
     2881 acagttagct aattggtaaa ataatgttta atttaaaata acagtaaatt aattagtaag
     2941 gcaatagtca attaataagg ggatgaaaaa atggatgcta aaattaatga ttgggttatt
     3001 atacacaata tagtattgac tccagaagaa agagctccac aagttccaga ggatactaag
     3061 aaagtatcct tagaaatgtg ggttaaaggt tttatacaaa gtgatgcttc tataggcgac
     3121 ttagtagaag ttaaaacaat tacaggcaga cttgtaaaag gtaatttact taaaataaat
     3181 ccatattata ctcatgatta tggtaaatgt ataccagagt tactccaaat aggaatccaa
     3241 gctaaagaaa tattatttgg aggtgtatat aatgaataat tcaacatcaa tgaaagatat
     3301 gagttatcaa gctgttatgg gaagaaataa cgaaataatg aaaaatgcaa tcggattgga
     3361 ttattcttat tttgaacaag agggaatagg gtttgattat gagaaaatga tgagtgaaac
     3421 aggatataca ttacaagata ttgaagcaat tcaatcacag tatgctgttg gaaatactcc
     3481 tttaatagag cttaaaaatt tgactaaatt agctagaaaa tgtgctaaag aaggtaaagg
     3541 agctagaata tttgtaaaag atgaagcaat gaatgcatct ggtagtttta aagcaagaag
     3601 agcagctact gcagtatatc atgccaaaca aatgggatat aaaggtgtaa tagcagctac
     3661 tagtggtaac tatggtgcag ctgtagcatc acaagcggca atgcagggat taaaatgtat
     3721 aatagttcaa gaatgttatg attctaatgg tgtaggtcaa cctgaaatta tagaaaaagc
     3781 acgaaaatgt gaggcatatg gagcagaagt tgttcagtta actgttggac cagagttatt
     3841 ttatacgttc ttagttttat tagaagaaac aggctatttt aacgcatcat tatattcacc
     3901 atttggaatt gcaggtgtag aaactttagg ttatgagtta gctatccaat ttagagaaaa
     3961 gtataaaaaa gaccctgata tagttgtttg tacaaatgca ggtggtggta atcttactgg
     4021 aactgcaaga ggattgataa aagctggttc tattaatact aaagtagtag gagcaagtgt
     4081 agatttaaaa ggattacaca tggctagtga taatcaattt aataaaaaat catttacaac
     4141 aggacataca ggttttggga taccatactg tacatggcct gataggtcag atgtacctag
     4201 gtctgcagct agaccactta gatatatgga tagatatgta ttagtaaaac aaggagaagt
     4261 tttttataca actgaattat tggcacagct agaaggtata gaaagaggtc cagcaggaaa
     4321 tacatcttta gcagcagcat tctctttagc tcaagagtta gatgaggata aagttatagt
     4381 ggtacaagaa acagaatata caggcgcagg taaacatata tgtccacagc ttacttttgc
     4441 aagagaaaat ggaatagata taaaatttgg aaatccaaga gaagaaattg caggagtaaa
     4501 cttaatatta ccagaaagac cagaattatt gaaatgtgtt gatattgata tgaataaaat
     4561 aagaaagtca tttataaaaa attgtatatc taataatcac attgataata ttgataagct
     4621 aagtaatcga gatatagaat ttttaatgga agaagtaaaa agttcaagag attttgttgt
     4681 tgatgtatta aataatttat agtaattatt gttttttaat ttaatataaa ataaaaattc
     4741 agaaaattcg attaaatcaa tacgaatgga ggaatgttca tgaaaaaaag agaggatgat
     4801 tttgaagtta gaagaaaaca tcttcaagaa ctgagtgatg atgagttaaa agaaaggttt
     4861 tggagtttag ctacccagat agtagaacca atgatagaac ttggtaaaaa gaatacaaca
     4921 ccatcaatag aaaggtctgt cttgttgaga atgggatttt cttctcttga agtaaaacct
     4981 attttagaag gggttatgga aagaggtctt ataggtaaag gagcagggca tgtagtttat
     5041 aaattggcta aatctaaaaa tataacggtt agagaagcag gtttgttatt agttaagggt
     5101 gaatattggg atgaagttac atgtttattt agagaagggg tagaatcatg ttaaaggaaa
     5161 ataaaaaatt ggatatagac tatatattaa aagatttgga taaatataaa ccaaaacgaa
     5221 gaggttgggt ttggagagaa cacttggaaa atcttgaaat gggacctttt aaatataaag
     5281 actgtacaaa acctttaaag aagagtgtag ggctaccatc atctaagtat ttcaataata
     5341 tagaccctca accaagtcca gttattacta ctgaaatagc ttctggtaga tttgaggatg
     5401 atataagaag aatgagaatg gcagctcatc atggagctga ccatctaatg gttatcagaa
     5461 ctgctggaca atcacatttt gatgggctta tagaaggaac tccacaaggt ataggtggag
     5521 ttccaataac aagaaaacaa gtaagagctc aaaggaaggc tcttgatttt atagaagaag
     5581 aagttggtag acctattaat taccattcat atgtaagtgg tgttgctggt cctgaagtag
     5641 cagtaatgtt tgctgaggaa ggagtcaatg gagcacatca agacccacaa tataatgttc
     5701 tgtataggaa tataaacatg gtaaggtctt ttgtagatgc ttgtgaggca aagaaaataa
     5761 tggcttttgc caatatagct caaattgatg gagcacataa tgctaatgct actgctagag
     5821 aagcatggaa ggtaatgcct gaacttatgg ttcaacacgc tttaaactct atattttcag
     5881 aaaaaatagg tatagataaa agtaatatat gcttatcaac agttccacca acagcacctc
     5941 cagcaccatg tttaaaaata gacttacctt acgctgttgc acttagagag ttatttagtg
     6001 attataagat gagagctcaa atgaatacta aatatatgga atcatcaact agagaagcaa
     6061 cagttactca tgtattaaac ttattaacat cagtattaac tagagctgac atacaatcaa
     6121 ctataacacc tgatgaaggt agaaatgtac cttggcatat atacaatata gaagcatgtg
     6181 atacagcaaa gcaagcttta gttggtatgg atgggcttat ggatatgata gaacttaaag
     6241 atgtgggtga attaagagac aaagcaagag aacttaaaga aagagctgta ttatatatgg
     6301 aagaaatatc tgaagttggt ggatattttg aatctgtgga acaaggattt tttgttgatt
     6361 ctggaaatta tccagaaaga aatggtgatg gaatatctag aaaaataaaa ggtggagttg
     6421 gtgaaggtac tgtttatgaa agagaagaag attatttagc acctgtaact gctcactttg
     6481 gatacaataa tgtagctcaa tatgatgaaa atgctataga taatccatca atgttaatag
     6541 atggatgtac atttgaaaat ccagacaaaa taatatatat tgatgagctt gacgattttg
     6601 ataatgtaga aaatagacta aaagaatcat atgagtacag aaatggaact aaaattaagc
     6661 ctgaaatgga atggtgtgca gatggaattg taatgataac aatgatgctt cctactgata
     6721 aaagaacagc agagtttgca gctttggaat ttgtcaaaaa aatgaactta caagaaattg
     6781 aggtcataag tagagaagtt atgcatgaat ctgaagggac tagaatagaa gtgaaaggaa
     6841 gagttccgtt tgatatagat ttaaacaatt tagtaatacc agaagaacct aaggtactaa
     6901 ctgatgaaga aataagagca gatatagaag aaaaacctat gaaaatagtt tcagctacgg
     6961 ttggagaaga tgaacattca gttggtttaa gagaaataat agatataaaa catggaggta
     7021 ttgaaaaata tggcatagaa tgccactatc tagggacttc tgtacctgta gaaaaattgg
     7081 tagatgcagc gattgaactg aatgcagatg caatacttgc atctacaata ataagtcatg
     7141 atgatattca ctataaaaac atgaaaaaat tacacgatta ttgtgtagaa aaaggtataa
     7201 gagataaagt aatgattgcg tgtggaggaa cacaagtaac acctgaaata gctgttgaac
     7261 aaggcataga tgctggattt ggaagaaaca gtaaaggcat acatgtagcc actttcttag
     7321 ttgaaaaaag aagagaaatg agtaataaat aatggaaaat agcaaggttt ctttaaaaat
     7381 agatgtgttg gtagctgaaa ttggaagtac tactacagtg gtaaatgctt tccacgatat
     7441 taatactaac aatcctatat ttttgggtca aggtcaagct ccaacaacag tgtttgaagg
     7501 tggcgatgta agaaatggct tatcaggtgc tattaaagat ttagctaata aattatctgt
     7561 agatgatata gagtataatg atatgtttgc aacttctagt gcagctggtg gattaaaaat
     7621 gactgttcat ggattagtct atgatatgac agtaaaagca gctaaagaag cagctttagg
     7681 ggctggagct gttattcgtc aaataacatc aggtagaatt aaaagaactg atttaaataa
     7741 aataaaagaa ataaacccaa acataatttt aatagctggt ggagtcgatt atggagaaag
     7801 agatacagct atttataatg cagaaatgat agcttctatg aatctaggca taccagttat
     7861 atatgctggt aatgttgaaa atcaagagga aataaggtta atatttgaag atactaatta
     7921 taagctttat ataactgaaa atgtataccc taaaatagat ttattgaata tagaacctac
     7981 aagaagaatc attcaaagtg tatttgaaga acacataact acagcaccag gaatgaaata
     8041 cattaaagaa atggttaatg aaaatattac tccaacccca ggagctgtaa tggaagcttc
     8101 taaactttta tataaaaata ttggagattt attaactctg gatgtaggtg gagcaactac
     8161 tgatgtacat tctgttacag atggaagtga ctatattaat aaaattttgg ttaatcctga
     8221 acctactgca aagagaagtg ttgaggggga tttaggtgta tatgtaaata tgaagaatat
     8281 agtagaagtt ataggtaaag aaaacttaca aagtgaacta tctatagata ttgatgcagt
     8341 aatagagaat tatcctccaa taccaaaatc aaaagaagaa atattatttg ttgagaggtt
     8401 gacaaaagaa gctgttgtta aagctatgct aagacattca ggaaaaataa gaaatatcta
     8461 tggaacaact ggaaaagtca aaattgcaga agggaaagat ttaacagaag taagatatat
     8521 agttggtaca gggggagcac ttactaggct ccctagtaga attgaaatat tagataatat
     8581 gctcaaatac aataaaaata atgaattact atttccaaag gaaaaaacaa agatattaat
     8641 agataatgac tatattatgg catctatggg tgttttatct aaaaaatatg aagaagcatc
     8701 tcttaagctc ctgttaaaaa gtttgaattt tgaggaggaa agattatgta tcctaggtta
     8761 gaaatagata tagaaaagtt aaagcataat gcaaaattaa tttcgcagat gtgtcatgaa
     8821 agaaatatta agatatcttt tgttactaaa tcattttgtg cacaaaaaga aatagtagaa
     8881 gaaatatgta gtgaaggaat agaccatata gcagattcaa gaatacaaaa cttaaaaaat
     8941 ttacaagata taaatctacc taagatattg attagaatcc caatgttaag tgaattagaa
     9001 gaagttatca attattgcga tattagtttt aactcagaat taggaacaat aaagaaatta
     9061 aatgaattat gtgaaagtaa aaatattatt cataagatag ttcttatgtt tgacttaggc
     9121 gatttacgag aaggatactt ttatgaagaa gactttttta gtaatgtaag agagatagta
     9181 agattaaaaa atattgaaat aattggtata gctacaaact taacctgtta tggagctatt
     9241 ataccatcta gggaaaatat aggcagactg gtaagtatag caaaaagaat ggaagaaaaa
     9301 tttggaataa atctagaaat agtatctgga ggtaattcaa gttctattca tctgttgata
     9361 aataataata tgccagaagg tattactaat ttgagaatag gcgagtccat tctacttggt
     9421 agagaaacgg catacggtga aaatataaat ggaacatatc aagatgcatt taaattaata
     9481 tgccaagtag ttgaatgtaa agaaaaacca tcagttccta ttggagaaat aggagttgat
     9541 gcctttagaa ataaacctgt ttatgaagat aagggaatct taaaaagggc aatcatagct
     9601 ataggtaaac aagatataaa tattgactct ttaattccaa tagacactga tataaaaata
     9661 ttaggggcaa gttcagacca catgatacta gatgtaagta acacaaaata tgattataaa
     9721 ttaggtgaca atttagaatt tttattaact tatgggggta tcatgtcatc tagtacgagc
     9781 aaatatgttt caaaaaaaat tatagtgtag tgtttaaata attaggagga aactatttta
     9841 tgaaagagcg agaatgtaga aaagcaagtc tgtttgatgc aatgatacca attacttgtt
     9901 tgatattatt tttaagtcta ggagtattag tgtatggttc atcacctcat gtaccactaa
     9961 taggagcagc agcagtagca ggacttgtgg ctgtatatag acttggattt aaatggaaag
    10021 agttagaatt aagtatgttt aatagtataa agatggcaat gcaagcaatc ttaatcataa
    10081 ttataatagg cgtacttata ggaacttgga tagtaagtgg agtagttcca tgtatgattt
    10141 actggggact aaaaatttta tcaccaaata tttttttagt agcatcaaca ttagtctgtg
    10201 caatagtatc tcttgcgaca ggttcatctt ggagcacaat gggtacagta ggagtagctc
    10261 ttttaggcat tggtcaaagt ttaggtatgc caattggatt aatcgtaggt tctataatat
    10321 ctggagctta ctttggagat aaactatcac ctttatctga aacgactaac ttagctccag
    10381 caatggcagg aacagatttg tttacacata taaaatatat gctatattcg actataccat
    10441 cattattaat ttgtttagta atttatggtg taattggaat gaagtattca ggtcaagcac
    10501 tagatataaa acaaatagaa ttaattaggt ctacattaga ttctacattt aatactttat
    10561 caccagtatt attattagct ccagcaatag taattggatt ggttgtattt aaagttcctg
    10621 ctataccagg gcttatagta ggtgctgtat tgggagtatt gtttgcagta gtttttcaag
    10681 gagaatctat gaatacgata ttagatgcag cacaaaatgg atatgtttcg tcaacacaaa
    10741 tacaggaggt agatgcttta ttatctaaag gtgggattat gaatatgatg tccactgtat
    10801 ccttaacaat atgcgcttta tctcttggag gtattttaga aaaaacaggt atgcttgaag
    10861 tagtagcatc ttctttacta agattagcaa agggtgtatt tggaacagta ttatgtacta
    10921 tggtaacttg tacaattacc aatataatag caggtgaaca atatctatct atagttatac
    10981 caggaagaat gtataataag gaatataaaa agagaggtat acatcctaag atgttgtcga
    11041 gagctttaga agatagtgga actttaacct ctccattagt accatggaat acatgcgggg
    11101 cgtatataac agcaacatta ggtgtatctg cactaacata tggtccatac gctttattaa
    11161 atattataaa tccaatagta tctttagtat taattgcatg taagtttaaa atagcaagaa
    11221 tagaagatga acctgaaact tgtttaaatt ttgatgtttg ataatagata acttatgtaa
    11281 gaacgtagga actagggggg tagcctgtgg ggaattagta agacatattt tagtgtgcga
    11341 aattctataa atcagtaatc ttgtgacttt agccatggga ggttcagatt agtaaattta
    11401 tagttttata ttagtctata ttttgatctt ttaattgttt gtataaattt tttatagaaa
    11461 aagatttctt ttagacttgc ctaaaaatta ggcaagtctt ttaagtatgt tctgattata
    11521 agagcactgt gtgacaagaa tgatgagatt atttttgcta tactttcgat ataagaaatg
    11581 gaaggaggtt atgttttata ttcatatgaa ataattagtt acacagtaaa ttttcgtata
    11641 atttggtata aaaagattag aaatgaaaat aaagtaatgg aggaataaaa tgactagaat
    11701 atccaatata acaatagctg aacaatctga gtactgtttt ttggcaatac gaaaaacaat
    11761 tgactttatg gtagagtttt cagagttttc aaaacaaagt tttgaaaaaa tttctaaata
    11821 tctggaggag agaggaattt tatcttcagg agcaccgatt gtatgttttc ataacatgga
    11881 tttagcaaaa ttggatgttg aagtagggtt tcctgtagcc acttatatag atgggaaaaa
    11941 tgagattcta caaaggattg ttcctgcaca gaaaataata actgcaattg atttaggacc
    12001 ttatgaatta caagacccta ctttagaaga cttattttca tgggcaaata gcaatggata
    12061 taaacttcat ggcgatatat attaccagta tttaaatgat atcaatcgtc cagctagtga
    12121 atatcttacg aaaatgatgt tacccattat atagaaagtt aaagattatc gtttgttagt
    12181 tgataattag aagaacattt actgctacta cataaatttt atcctaaata atatcaaggt
    12241 gatttaattt atgtattcta atgtaacccc aatatattgt taaggtctac ttaagatgct
    12301 caaaaggcta tgcttaattt attgttatat aatttgtaga gtggtataat ttagaggtaa
    12361 tattatatag atatataggg ggaaaatatg aaaatatatg atatattaac acgtttttat
    12421 attcaagata tagaaaaagc catacctttt tatgaaaatc ttttaaaaga aaaatgttct
    12481 ttgcgttttt cctataaaga agttgggttg gaattagcgc aaataggaaa tgttttacta
    12541 ttatctggtt ctgatgatgc tcttaaacca tttatagaaa caaaatctac ttttatggtt
    12601 gattctgttg atgaatggag aagttatcta ttggataatg gagcagttgt agtaagagat
    12661 aagaaaaaag ttcctacagg atataatatg acattgagac atccagatgg tacaattata
    12721 gaatatgttc aacatacaaa gcaggattag ttaaaaacag attttactac tgagcaacta
    12781 attagaatct attcttagac gtacaattta ttatttttgg tattaacaga ttataagtca
    12841 aattataata tttcaaaata gaatagaaat ttatttacat aaaagagagt gtttaaaaat
    12901 gaacttttta gttcatgaag cactcttttt tgtatgaaat caaatacatc aatatataaa
    12961 ataaactgat gaagagctta aattaaaata aaaagattca taacaggacc ccattaaact
    13021 ttaagatagg gtaagtttgt aatcatatta aataattgta aaaagctgtt tttagtcgta
    13081 taatataa
//
LOCUS       DAMJYX010000092        12958 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJYX010000092 DAMJYX010000000
VERSION     DAMJYX010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.094"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(73..282)
                     /locus_tag="PC479_002799"
                     /pseudo
     CDS             complement(73..282)
                     /locus_tag="PC479_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
     gene            complement(384..932)
                     /locus_tag="PC479_002800"
                     /pseudo
     CDS             complement(384..932)
                     /locus_tag="PC479_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="PC479_002801"
     CDS             complement(1291..3201)
                     /gene="phnW"
                     /locus_tag="PC479_002801"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2805330.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(3480..4265)
                     /locus_tag="PC479_002802"
     CDS             complement(3480..4265)
                     /locus_tag="PC479_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2805331.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="PC479_002803"
     CDS             complement(4482..4712)
                     /gene="dltC"
                     /locus_tag="PC479_002803"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2805332.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="PC479_002804"
     CDS             complement(4731..5879)
                     /gene="dltB"
                     /locus_tag="PC479_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2805333.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(5879..7393)
                     /locus_tag="PC479_002805"
     CDS             complement(5879..7393)
                     /locus_tag="PC479_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2805334.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="PC479_002806"
     CDS             complement(7514..8704)
                     /gene="dltD"
                     /locus_tag="PC479_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2805335.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(8992..10011)
                     /locus_tag="PC479_002807"
     CDS             complement(8992..10011)
                     /locus_tag="PC479_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2805336.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(10187..10726)
                     /locus_tag="PC479_002808"
     CDS             complement(10187..10726)
                     /locus_tag="PC479_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2805337.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(10827..11507)
                     /locus_tag="PC479_002809"
     CDS             complement(10827..11507)
                     /locus_tag="PC479_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2805338.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(11536..12825)
                     /locus_tag="PC479_002810"
     CDS             complement(11536..12825)
                     /locus_tag="PC479_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2805339.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         4177 a         2330 c         1243 g         5208 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac aagtgtgcat
       61 agctattttt taatcaagag aagtctcttc tttcatagac aatattaact ctattctttc
      121 tagtatttta tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa
      181 ccattcctct ggtattatgt tttttctttt ccatctatat aattgtccat aagatatatt
      241 agttttgctc aataaatctt tcttagatat taattcttcc atagctcttc tagcccacct
      301 ttatactgtt ttttacagta gaattatctg aaactgataa attatcactg tattcaatat
      361 ttccaacatc ataattatct actagagata tgcttttttt ctagcatacg aaattttttg
      421 caataatctc catgcccctt ttttatattt atactacttg ctccaacttc attaaaatta
      481 gaagttccat ttaaactaat attaacttct tcacaattca aaaatccatt acactccaag
      541 attccactta aattgatatt ttcacaatct atattctcat ttgcttttaa atatccttct
      601 ccatgaataa tatccgattt tatacttcct tctacattaa taattccact agtatcaatt
      661 tcaacagaat taatatttcc tgtacatcta agaagtcccg atatctcaac ttttgatgcc
      721 agtatgtttc cttctacttt acttaaacca gatgtaaaaa attcattaca ttctgttgtc
      781 ccaaaaactt ttgctgaacc agaagaatca aatttactac attttacact tccttctatt
      841 ttgcctgttc ctgatatatt aacattatca aaaattccac ctttacattt tccaacacca
      901 tcaaatctta gtgaacttaa ttcttcattc ataatgatat cctttcttta ccgtatttta
      961 tctttaaacc taatataaca aaacattgtt acgttgtcaa gtatgataat aatacttaat
     1021 aaagcaacta tcatttattt tctataaata ggcataaaaa taatagggaa atgctttatt
     1081 taaactataa atctaatttc ttagattgtg gtttaaataa aatatatccc tattactcaa
     1141 tgtttatata ttcattgtat aaacttctaa aatataagtc tttaaattct actgtcaata
     1201 ctttttatac actgtttgtg ctatcatctc acacacctaa aggagtaggc tttgcgcact
     1261 attctgtaac ctacaagaca ctatcttctt ctatctattg ataaattttt ctattacatc
     1321 agctagtctc tccatatcag ttggatagac ttcacctatg ctacctattc taaatgtatc
     1381 tatatcagtc aatttacctg gatagataac aaatccatta tcttttaaat aagtataaaa
     1441 ttccataaat tcaaactttg catttttagg atataagaaa gttgttatta caggtgattg
     1501 agcattatca ttcaccaaag tatcaaagcc taaagatttt aatcttgaag ctattgtaaa
     1561 ctgattttct ttatatctag catatctttt ttcaacagaa ccttcttctt ccaattctaa
     1621 taaagcttga taaaaagctc ttacaacatg tgtaggtgaa gtaaatctcc atttaccatt
     1681 atttttctcc atagtctccc attgcgcata cacatcaaga gataatgatt tagctatacc
     1741 tttacattta gataatttct ctttattagc tattataaaa ccaaatccag gtactccttg
     1801 tatacatttg ttagaactac taactaaaaa atctatatta aaatcttcaa catcaatctc
     1861 aattccccca aaactactca tagcatcaac tatataaatt ttgttatatt cttttgcaag
     1921 tttccctacc tcttgtatag gatttaatct acctgtagta gtttcacagt gaaccattga
     1981 tatatgtgtt atatccttgt tttcttttaa taaattttct actacattta aatccactgc
     2041 ttctatatct ttaaatgtgc agtcaacaaa ttgtatgtta agataattac atatatcttt
     2101 cattctcttt ccatatgctc catttgcaat tacaagaagt ttaccatctt ttgaaatagt
     2161 acttcctatt atagcttcta ctgaaaaagt cccacttcct tgcattaata cacttgtgta
     2221 tttatctgta ttttgggttg ctagagatac taatcttctt ctaacatctt gtactaggtt
     2281 attatattca acatcccaag tacaccaatc ttttaacata gatgctctta ctgatttagt
     2341 agtagatagt ggtcctggtg taagtaatat gtaatcattt tctggtacaa aatctgattt
     2401 aatagtttca ttctctattt ttattagtat atcttctaac tcagccatag tttctattac
     2461 aaaatgtgca cctgcctctt taaatttctt tgaaactata ctcatcttag cttttaattc
     2521 ttccttatcc atgttctcta cttcttcttg tgtaagccct aactcacttg aaccttttat
     2581 tacagcaaca ctccacattc ctgcatttac gccttctttt acatcactta ttgtatctcc
     2641 tacttttacc atagaactca taggagacac ccctaaagct tcagcatttt tatagcacat
     2701 ccaaggatac ggtcttcctt gagatacttc tgatggtgtt acaagaaaat cgggtgagta
     2761 tcctttcttt gctgcatttg gttcaactat gttcatcatc tctcttgtat aacccgttgt
     2821 agaacctatt tttaatccat tttttctcaa cttttcaata gtttcaacaa catgaggaat
     2881 tggagtcgta tactcttcta atgtctcaaa taacattggc tcaaattcag cataaagttc
     2941 atttacatca tcttcagttg gcactttacc aaacttatct gaccaaatat tctttattct
     3001 atccatttca cacatttctc ttatatggtc tatttttaat tttcccatag gttttcttgc
     3061 ttcttccatt gttacatcta tacctcttct tttaaatatc tctataaata cattaagagg
     3121 tgcaaaacat ccataatcta ctgttgttcc agcccaatcg aatactactg cttttatttt
     3181 ttctccatat atctttttca tatttatagc ctccatagat tctttttaga atttttgtgt
     3241 ttttaattgt ttttactttt aaagtataac tttcatatgt taattataca tcatatttag
     3301 gttaaatata tattaaattt ctgtttttag ttgtatttag ttgtttttat tttgtatcat
     3361 attcccataa aatcctaagt tacaatctaa aatttacttt ctaatcttaa tctatgaact
     3421 gactatattt tatgaattta gttgtattga gcatagttgt attatataaa actaagggtt
     3481 tattttgctt ctatccatgt tatatttatc tcatttaact tatccttcca ttcattcgga
     3541 ggttctacat cacaaataac aaaatctaat tcctctaaat ctgctatctt atacatacta
     3601 gcaattccta ttttagaact atcacataat acaaaacttt cctttgaatt ttttatatat
     3661 tcttttgaca acaatgcctt attttggtct atactactaa ttccaaaact atcaagtata
     3721 ccttcactag atataaaagc tttatctacg tataagtttt tcatagtatc tattgacatt
     3781 ggacatgctg tcctttggtg ttttaaattt acttttccac caataaatat aacttctcca
     3841 tcaaacaaac cttgattctc ataagaaatc aattttgata gaactggata agagtttgta
     3901 ataattttta aattgtttac agatattata taatctacaa tctgcaaatt ggtagtacct
     3961 tcatctatga taattgactc attgtcattt acaaacttag ctgcatattt agcaatactt
     4021 tgctttttat ctaggttagt attgtttctc tttatatgtt ctggttcaat cactccataa
     4081 tttattttta tagcaccacc atataccttt tttaatttat tttcattttc taattcttcc
     4141 aaatatcttc ttattgtttc tgatgatact tctaatcttt ttactaattc tttagtgttt
     4201 accttccctt ttgaatctaa taatttaagt atttcttttt ttctatctat cgaatttaaa
     4261 gacattcatt ttcctcctaa atataatata tttttctcct ataatattac aatgatacct
     4321 aagatttatg aaatcatatt tatgtattaa attttttact taattaaagt tattttcaaa
     4381 agactttgta ttatcattaa aaaagtccat caaatattag tatcagatgg actttgtaaa
     4441 attttatttt ataaattaat atttaaagtc taactactaa tttattttct tgtctccaag
     4501 aattttacta aattatttac agtagacata tcttttctct ctaaatcagt tggttgaagc
     4561 tctatcccta atctattttc aatttcaagt aaaacttcaa ttatagctaa tgagtctaaa
     4621 agttccgtct caaataaatt aaggtctaaa tcttctctta tctcatcagt ccctaataca
     4681 tcttcaaata tttctataac tgtttcttgc ataaattttc ctccttaaaa ttaagctaaa
     4741 taacctgaga atatcagcaa tccaaaacac acaatatgga atgtaatgaa aatctgcact
     4801 ctctcgaacc atttatcttt tttatgtttt ttataaaatt tagattttcg ctgatatata
     4861 tcagttaaca caagtgccaa tccttggtat acaccataag caacataata ccaagttaga
     4921 ccatgccaaa atcccattgt tatcatggtt atcatctgag ccacatgagc agcagtagtc
     4981 cttttcttaa atcttttctt tctcattgat gacataacaa atctagaaaa aatataatct
     5041 ccaaaccatc ttgaaagact tatatgccat cttgtccaga actctttcat atctttactg
     5101 ataaatggct tgtcaaagtt tataggtact tgtattccaa atatgtatcc tgtacctata
     5161 gcaaataaac tatatcctgc aaaatcaaag aacaggtata aactatatgc gtacatataa
     5221 cttaaaatat ttataaaact catatcttta ggtattcttg atacccaata tgtatttatc
     5281 aaaaatgcta taacaaactt ataacctact cccatgacaa tattttttat tcctggcagt
     5341 aagtattctt ctatatactc ttttctagag atttgttttt ccaagtcctg ttcaaatctt
     5401 cttgacctat caataggtcc agaacttaaa gttggaaaaa ataacataaa ataaagcatc
     5461 tttgaaattt taacttcttt tatagctcca tcataaattt caataaccat ttgtatagtt
     5521 ctgaaattta agtatgatat tccaataaat ccaattattc caaaagaagt aactggagat
     5581 attttattta ttattattgg gagcattgat gcaaacaaga atccccaata tatgtattta
     5641 tttttagttt ttcttcttac atattcatat cctttaacta ttattacttc aagaataata
     5701 aatattacca agtactttag ttggacatca attcctacta ttaagaatat cataaacaaa
     5761 cttgccaaca taccataata ctttatattc ttgcccatta gacctaaaat tactgctggt
     5821 attgatgtta ataataatat ataaagataa aaataatctc catattgtga aaaaatcatt
     5881 aaatttcctc cataagtttc tttctgtcta ttttaccatt gatatttgtt ggaaactcag
     5941 agattattga aatatttctt ggaatcatat atgaaggtat atactttcca agttcctttt
     6001 ttattatcat cccattttta atattactta aatcattctt ttcatttaac tcaacaatac
     6061 cttttaagta agcaattttt tcgtccttat atactggtaa aactactgca ttttttacat
     6121 tatgtacttt tctcaaatta ttttctatat cttctatttc aattctaaat ccatttaatt
     6181 taatctgaaa atcttttctt ccacaataat atatatttcc atctaaaaga tatcccatat
     6241 ctccagtttt ataagctctc cactttacac catctatttc atcatagaaa aatacttcat
     6301 cagtcttttc tttattattg aagtaacctt tagaaactga tggtccaatt attataattt
     6361 cacccttttc attttcttta agctcatttc catcttcatc aagtattttt attttacaat
     6421 ttgacatagg gtatccaact ggaagacttt tttcatcatc tatagccttt tgagtcatat
     6481 catttacact tacaccaaca gtagcctcgg ttggtccata tccattgata attcttgtgt
     6541 ttggaaatct actcataagt tctttagtaa gattttttga aagtgcttct cctatgaata
     6601 tcattgactc aaggttagga agcattttac tattaaactc tttttcagtt atacatactc
     6661 ctgcaaaaga aggagttgac acccatacag ctatatctga gatactaaat tgtctaaata
     6721 gttctttata gtctgctaat acatcttttg atattgaaaa taatgtagct ccatggataa
     6781 gaccagggta tattgttgta actgaaaggt caaaagaata tgctggttgg ttcattatta
     6841 ctttttcact tccatctatg tttagatatg ggcttatcca atctgaaaag ctatctaaat
     6901 tgtttgaact tatttgaacc ccttttggct ttcctgtact tcctgaagta aatagtatat
     6961 atgcattttc atcatctttt acccagtttt ctttatctaa tgattttccc tgatattcat
     7021 ttattatata atttaattta tccatatcaa ttacattgat atctccaaaa tctttttcat
     7081 cactaaagtt aaataatacc tttggtttta tctctttagt gacttcaaaa actctatcaa
     7141 ttggaaaact aatatcaaga ggcacatacg ctctacctga ttttaaagct cctatcatac
     7201 atgccattat catgttttct ttatttccat aaattactat aggagtatct tcttctttgt
     7261 atacatcctt tagaaatact gatatagcat ctgaatattc atttaaatct ttataagaaa
     7321 gtttatctcc attacacatt agtgctgttc tatctgtatt tgaatatttt tttattcctt
     7381 caataatttt catataagaa tgctccttta aaatccgtta taaataaatg gcaattcttt
     7441 aaatggagta aatgttagaa ctcctatcgc caatattata accatagtta aacaaatact
     7501 agttaacaaa tctttattgt tctttaaata ttttaaataa tctctcacag acatctaccc
     7561 atcctttcgt accaagatgc attacatccc ttaaataata cttatctgac cctttatctt
     7621 taaggtttag aacttcaaag cctttgcttt ctgctatttt ttgtgcttta tcgtagtact
     7681 gatttctttc tttttcagaa ataccagtca aattatagaa tttatccata cttgggatta
     7741 atacaacaac tggcttaata cctaagtcag tacatacatt tagcatcagt ttataatttt
     7801 caaactcctt agatgttaat aaatttacat ctttgtctct tcctttgtat tgctctatac
     7861 catctttaaa atgctcttta taatagtact tatcaatatt tagtggattt ttaccaactc
     7921 tttttttagc atcttcaatg gctttctttc tctcatatga ccaatttatt ggtctttttc
     7981 tttttgttgc tctcttttta tctagcttaa ttaatcgttt ataaagaata cccttttctt
     8041 taagagctat acaatacttt cttccttgaa aataaggctc taaaagcacc ttttcagctt
     8101 tttcaggaaa tgtcttaggc tcatatagct tagcatataa tgcttctgct ttatactcgt
     8161 cacttcccca taataactta cttgattttt ttgcatattc aatcttattt tgtttagata
     8221 tttttggatt atctaaaaat cgataaaatt gtattggtga aaatctagac tgataatggt
     8281 gacttgtgac tccgtctttt tccataaacc attgcataga aattaacaag acaactttct
     8341 tattatcaat attaggattc atacttccta atattgcagc atcttgtaat gtctgagtat
     8401 aggctctccc aattgcaaac accttatttt tactcctatt tgtattaaaa tagtactttg
     8461 gatgttgttt tgttgaatta ctaagttctg atgaccccaa aatcataata tccttttctc
     8521 ttaaaaaatg attatttgct gtaacccctt tatcttttat atcacttaaa gtatcatcca
     8581 taattggaag tagatttttc tccctcaaaa gttcatcagt tttagaatca agaaatttgt
     8641 ctaacccaaa taaaaatact acgcctatta taaaaggtgt aataaagtat atcaattttc
     8701 tcatattttc tctcctctaa aaatattcaa atttatgtta ttattaatca tattagtttt
     8761 gatatgtata ttcaactaca aaattgtaat aaaatcgtca aaactttttt tgatactgtt
     8821 acaaaattgt aaaaaaaaag aaaaaaacgc caaaaataaa tttcttttta gcactttttc
     8881 atacttataa tttgacattt taacacttgg ttaagtatac accttacctt aacttaaaat
     8941 taaatttatt taagaatata tattataaat tcttatattt tctaatataa actatatact
     9001 atcagctgga taaacaatac tactttgtct tcttatctca tccataattt ccattactat
     9061 tattgaattt tcatgagaat ttatatttga ttctatttta ttgttattta taagacttat
     9121 aaactctaca agttcataat acattccttc tatttctcta ggcttttcct ctgctttttc
     9181 ttttggaata gatatatctt cttctcttcc atctctgtat ttaataatag ctttttcaaa
     9241 tagattaagc ttttcaatta ttatacttcc ctcttctcct tgtatttcag atggtatata
     9301 agaatcagct actttagaat actgaattat cgcatccata tcatcatatt gaaatacagc
     9361 tgaaccttct ccatccactc cagtttgaag tatatgagaa actgccttta cagtttttgg
     9421 agcaccaaat aaattcacca taggatgtat acaatacact cctatatcca ttaaagctcc
     9481 atttgacaac tcttttttaa aggcattttc tataattcca tttttatatt tatcatatct
     9541 tgaagaatac tgacaatagc ttccaaaaaa tcttcttatt ttccctattt tatataagtt
     9601 ttctttaact gctttaaagt ttggaacaca tgtaattctc atagcttcca taagcaatac
     9661 attattatct ttggctgttt ttatcattga tttgacttct tttaaattag atgctaacga
     9721 cttttcacaa agcacatgtt ttttgtgttt taaacaagta atggtttgct ttgaatgtaa
     9781 tgcatttgga gaagctatgt atacagcatc tatatccttc gatttagcca tttgttctaa
     9841 atcatcaaaa aaaatacttg ctccatatag agttccaaat tctctagctt tttctaaatt
     9901 tctagaatat acagcaacca attcaaagtc tttaacccta gatgcagctc ttagaaatat
     9961 atttgctata ttgcttgtcc caacaatacc aaatcttatt ttacccatca taaatcactc
    10021 cccattttct acactttatt ttaattttaa taaaaaactt tacaacttat agatatatta
    10081 taaaataatt ttatcaaata aaaaagaaag ctaattttaa atgatttcac cattttaaca
    10141 tagctttctt ttaactacaa tctaaattca acttatattt ttaaagttat ctttctacta
    10201 tatctttatt tcccctcaaa aattttatag taagaatttc atccacaaac aatgttacta
    10261 aaagtatagt tacaacaatt ggccaataag gtattataag actatataaa ctatcaacta
    10321 tatacacttg aaaatatcta actccaataa atgtcaatat agtccaatat acagaaaact
    10381 ttagacatac tattcctcta ctattgtact tataatctga ataatcccaa tagaccttat
    10441 taaaatatac tttcataagt attcctgtaa tatattctac tactgtcggc actactagac
    10501 ataataaaag caatgcaaat atattattgg ggtcaataaa tcttgagacc tctattataa
    10561 tcgacattgc aattgcatac attggtttaa atggaccatt taaaaatcca tcttcttgaa
    10621 aatgcccttg tttataataa ctatataagt tttcaattat ccaacctata aaaccataca
    10681 agaaaaaatt aaataaaaaa tataatagag aacctacatt atccatgaat attcctccta
    10741 gctgtttata cttatttttt ctcatattca tggatttatt ctaaatcaaa gaaattatat
    10801 ttaatttatt taattaatac tttatattat agattgtgac attctttaga gttcaaactt
    10861 tttaggtatt ctctaacttc cagtaaattt tcaagtatct tatgtgattg tgatttaatt
    10921 tgctcatctg gctcttttaa atctggtaca tttatacatc ttataccacc attgtatgca
    10981 gcttcaactc ccataggcga atcctctatg acaatacaat tttggggatt aacatttatc
    11041 tttttagctg cttttaagaa tatctcaggg tttggtttag agtttacaac atcatctcca
    11101 caaactatag catcaaagta atcttttaaa tttgccttag ctaatctctt tactgctctt
    11161 tctctctttg ttgatgttgc aacagccatt ttgtatccat tttcctttaa aaaagagatt
    11221 agttcattga ctcctaattt gataggagct cccttcctct ccataaattc aatcatattt
    11281 ttagtttttt cgtcatataa atctattatt ggtacagaac tatcgtatat atctgtaaga
    11341 ccttctatta ttcctttacg atttcttccc ataactgatg tataaatttc cttagtcata
    11401 gtatatccat acttttcaaa cgtctccatc caaaattcaa gagaaattct ctctgagtca
    11461 aacaaaactc catccatgtc aaaaataatt ccttctactt tttgcataca attcctccaa
    11521 tattttttga tataattata ttacactttt tatgattaat tttacaaccc tcaatttatc
    11581 caattctaat attgtaaaat tgtaattttc aaaagttaca ctttggttta ctttaatatt
    11641 tgaaccaagc atataggata tccacccacc tatactgtca aatccatcat gctctatatc
    11701 aagtccaaaa tacttattaa tatagttgat agattcagtt ccatcaacta agtaagaacc
    11761 tgctcctatt ttcttaatat gagtctcttc tttgtcaaat tcatcttgaa tttctccaac
    11821 tatctcttct agtatatctt ctattgttac cacaccagat gtaccaccat attcatccac
    11881 aactatagca agttgaagtt tttcctttct tattctctca agagctttat ctatagttaa
    11941 attttcagat atatatataa tatctctcaa tatttcttct agttcaattt tattttcatt
    12001 tatcttttga ttgtataaat ctcttatatg tacaaaacct aatatatcat ctttattttt
    12061 tctgcatact ggatatcttg taacaccttc ctcttttaaa atagctaata tcttttcttc
    12121 agaatcactt tcatatatac ataccatatc agttctagga accattatct ctctaatttt
    12181 cttttcttca aattcaaaaa tattatcaac taatctttgc tctgattcat ctataagtcc
    12241 acttttataa ctttcttcta ctaatagtct tatttcgtca cctgtatggg gttcatcaac
    12301 ctcatcagct tgtgaatatc caaatggttt aagaaataaa tcagtactta agttgaaaaa
    12361 atatattatt ggatatgtta acttataaaa tcctactaat aaaaaagatg tactcaacat
    12421 tattttttct gtattataaa gtgctaaagc tttaggaacc aactctccta ttacaacctc
    12481 catcattgtt ataattaaaa aagaaatgga aattgatata gtatatataa tactttcact
    12541 aagattaaat acacttacta ttggtaaaat taactcttta actgtagatt ctcccatcca
    12601 acctaatgca agtgaacaaa gtgttatacc aagttgacat gcagaaagat aggaatttaa
    12661 gttgtctttt attattttac atctttttgc attattatta ccttcagtta tcatggtatc
    12721 aatcctagaa ctacgaagtt ttaccatagc aaattcacaa gctacaaaaa aagcatttat
    12781 caaaacaagc attactataa gtgttacttt taataactcc cgcataaagt attggttctt
    12841 ataatgtaag aaatctataa gaactcaaag ttcccccctt atattaataa atttaattta
    12901 taatatatat tctatcatat ttattaatat atcctacttt attacaaata atttttat
//
LOCUS       DAMJYX010000093        12951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJYX010000093 DAMJYX010000000
VERSION     DAMJYX010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.095"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(448..699)
                     /locus_tag="PC479_002811"
     CDS             complement(448..699)
                     /locus_tag="PC479_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805340.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(945..1100)
                     /locus_tag="PC479_002812"
     CDS             complement(945..1100)
                     /locus_tag="PC479_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2805341.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            1811..2035
                     /locus_tag="PC479_002813"
     CDS             1811..2035
                     /locus_tag="PC479_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805342.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     repeat_region   2322..2614
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2387..2415
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(3570..4997)
                     /locus_tag="PC479_002814"
     CDS             complement(3570..4997)
                     /locus_tag="PC479_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF2805343.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            5188..6009
                     /locus_tag="PC479_002815"
     CDS             5188..6009
                     /locus_tag="PC479_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2805344.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            6134..7651
                     /locus_tag="PC479_002816"
     CDS             6134..7651
                     /locus_tag="PC479_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2805345.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            7674..7958
                     /locus_tag="PC479_002817"
     CDS             7674..7958
                     /locus_tag="PC479_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2805346.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(8055..9725)
                     /locus_tag="PC479_002818"
     CDS             complement(8055..9725)
                     /locus_tag="PC479_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2805347.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(9797..11044)
                     /locus_tag="PC479_002819"
     CDS             complement(9797..11044)
                     /locus_tag="PC479_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2805348.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(11031..11705)
                     /locus_tag="PC479_002820"
     CDS             complement(11031..11705)
                     /locus_tag="PC479_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2805349.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(11877..12935)
                     /gene="mltG"
                     /locus_tag="PC479_002821"
     CDS             complement(11877..12935)
                     /gene="mltG"
                     /locus_tag="PC479_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2805350.1"
                     /translation="MKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIIDVPSGASV
                     GKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISLLVSGKIY
                     QDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKEDGITSLE
                     GFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVMEMASIIE
                     KEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKIDSPYNSYK
                     NKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKYVKEYKEA
                     RDKQSKDTKATNKENTKK"
BASE COUNT         4429 a         2027 c         1471 g         5024 t
ORIGIN      
        1 ctacctttat attcttactc tgattttata tctaatatta attcttcatc aaagctatta
       61 aatatacctc taattgagtt aattatttca cttacttcac ctttaatttt tatattaact
      121 tcgaccattt caaatctcat ctttttatct tataatttct ataagttttt ttatttattg
      181 tgttgctact ttgaccagca cttttttatt taatagtcta aatgatattt tttaccagtt
      241 ttaatatcct taaatactat atttactaaa aacttataat ttcttttaat acatagttaa
      301 tatattctta ttactaataa caatactcaa caattttatc gccttttttc aaatatgatt
      361 taactttttt attcctaatt atttaaaaat agttgtccaa ttttcttcta tgagaaagtg
      421 ctaagtatta gcaacttctc tattctctta actttctact gtctcaactt tcttcatagc
      481 tatattaata gctacttctt tagctacttc attgaactct tcccatcttc tcctattttc
      541 ttcatcagat atacgtggat atactatata aacttctgtg ttttgattag ctataatttt
      601 ttcatcatat tctatatttt tttctttatc atatttgtat ggtttaaatt gttgtagtat
      661 tttcataaca tcaccccttg ttataaaata tgaactcata aacttgtctt atacttattt
      721 aattgttaca atttttactt aaaatcactt tatattctat attttaaagc taaatccttt
      781 acaaaagtaa catattcttc tataagtttc ttatcctctt acatagtatc tagtttgttt
      841 aacttatctc ttttaaactt acatacgcct tcttctgcca ttcgtctttt attaattgtt
      901 aaccttgttt caagagacac accaaaactc ttgttaagaa gctcttagct ctcattagca
      961 aaatatttta cataacttaa atcaccttga cttctagcta ttttaactgt taatagactt
     1021 gtttcttttt tccaatgtgt tatatttaat ataacaacat cttttattat tttaacttct
     1081 atttttacat ctaatgccat attattgctt tattaatttg tatccttaaa tcttaaattt
     1141 tctattaata catcttctat acagcttagt ttctctcgct taatcttgaa atatattttc
     1201 tctaaattgt caaattgttc ccaagtttta tcaatatcta atattttgca gtgtctattt
     1261 gcacatcttt cgatccaaag gtataattga ctagaatgtt ttgaaatcaa ctgactatta
     1321 atagattagt tcttaaattc tttcaaatac tcgaattgta ataaaacata atttacattc
     1381 tctttatttc tattgaagtt agtttatata ttgtttacat cattttcata tatatctgct
     1441 agttgctatg catttaaaat tctcttatta tttctttata ttacttgtaa attattcatc
     1501 ctcatcatcc tcttaattaa atttctatct tagaaattta atttgtaaaa aaattttcta
     1561 tatcataatc aagtatctta gatagtttat aggtaacata tcaactctaa ttgaatattc
     1621 ccctttcctg tacttaaaat aaattgatgc atttttaaaa cctaactcat ttgcaacact
     1681 ttgtaagtat aaattttatg tatgtcttct tttttataaa atcatagtgt atctttttca
     1741 tatattcaca ttaattgcta tatttcaata acaatatttc caaaacagaa acttttcgag
     1801 ataattatgt atggatgtaa atcaaaggat taaatatatt agggaaaata tgaatagttc
     1861 tcaaaaagaa ttgcttgaaa aaacaaatat aaatacaagt gttatgaata gaatagagtc
     1921 tggtgaaaga ccaacaagag acgatgggtt aataatattt gctaaaattt ttggtttata
     1981 tactgattat attcttggtt tatcagatac agaaaaacta aatattggca attaaagctg
     2041 aattaattgt attatatgtg tgatgaatat aaatagagaa ttttttgaaa agcttgaaat
     2101 aacttagaaa aaataagagg ttcactcaag aacaagttgc taaagattaa aaaacaacaa
     2161 aggtatctac taaaagatat gaaaatagtt taagagagtt gagtctggaa atatttatgt
     2221 gtattcttta aataaaaatt tattttttaa atgataatta aatttttact gatatggcta
     2281 tgcttttaaa agtcctaacc ttattttgat attatatctg tctttacata ccacttaatt
     2341 aatataaaac agtataaaaa ttagttacag atgtatcttt tttgttcttt acataccact
     2401 tagttaatat aaaactatta attttcctat gcttgtactc atactcaaaa agctttacat
     2461 accacttagt taatataaaa cgattgttat tttacgaaag gtacggtcgt aatttacgac
     2521 tttacatacc acttagttaa tataaaacaa gctagcgggg atagacaata taaatatcaa
     2581 cacttcttta cataccactt agttaatata aaaccccaaa ataaattgag catttccaag
     2641 acttacatct atacaattct ctcaaatttg cagtgaacca cgagtagtgc aaatgataat
     2701 atttatcata taccctcaat gccttatgtt tcaactgtta agccctattt tatcaaaaat
     2761 atcgctcact gcaaaactat ttataatatt atgaatatat tatttttaaa tatctgtacc
     2821 aatttgtagt ataataaaat caagaaaata atttactcct ataaagagtg attattttta
     2881 agtgaaaata ttattttttt aaatcattct tattggcgtt tgggagattt ttattttatc
     2941 ataaatataa actgatataa caccagctga catgcttgcc actttagatt attattcttg
     3001 ctacaactat tataacatat aaaacttact tatttttacc aaatatttta tgtaaacaat
     3061 aaatattaac ttatgaaata tctgctcatt tacagatatt taataagtta atagctctat
     3121 aataaaattt ttatcattgt ttataaaaaa ttttactttg cattttttta taaacaacag
     3181 ttttatttat aatgttttgt ggtataataa aagcaagaag aactacaatc tattttgcgt
     3241 tagagtggag ttcatcataa acagagttta ttttttggat ttaaatataa atttaaattc
     3301 aactcgtaag tcactctttg tgcgagagtg gctttttgct tttttgaata gtttactaat
     3361 taagtaaact accacactag ctgttaaact agctaaaaca ttaaacaaaa agttatccat
     3421 gaactcacct cccttcgaat cgttgggagg ataatctttt gtacatgaac tccactctat
     3481 agattgtaag ttaaatcttc ttgctaaaaa tattataaca tattatggta aattatataa
     3541 ttaaaaacac ttgtttaaaa gttctaattt taattataaa ctttcccagt ttctatatct
     3601 ataaaaaacc aaccaaaagt accattacta ttttttgaat tgattttaac agcatagaca
     3661 ttaccactat atatatcatc tctaaaatct atcatatcaa ctactaagtt attgatactg
     3721 actttatgtc tcttagcaca ttcttttaat gctatctgtt ttgcttgttc ctctgttata
     3781 cttcctttat ttttataatc atctggatta catactttca tattaccatt aacatcatat
     3841 gtataaactt tgctcatatc atttttagct acacagtatc taacatcacc ctctgcaccc
     3901 tgtttaatgt aagcaaatac atagtactct tttcctgaaa aatcaaaatt tttatctagt
     3961 agaaatatta aatcttcatc ttctgaataa ttaggttcgt aataaaattt cttcacacct
     4021 aatagttttt tcactgcatt agcagcatct tctactgtat attctcttcc agtattagaa
     4081 tcgcctatat tttttgttac atttaaacac tgctgtaata tactatcact caaatttcct
     4141 atagcagtta atttagttgt acttttcaat acacttttat tactatcatt atcaactaac
     4201 acaatagcat tatttttagc aacagtagaa ccaacaagag catacactaa atcagtacca
     4261 cttgctatat aaaattcttt tactccacta taaaatttat ttattatctt tttgttagta
     4321 tcatatctat ctgtcccacc taatctagtt gaattagtat ccttaactat tttgtcattc
     4381 attgatgaag taccacctat tacataactt tctacaccag ttgtattaaa tggaacactt
     4441 tttccatcag ttaatactat agctgcttta tctctaactg ctacaggtgc agcactcatt
     4501 gcatcaggct ctcctttaaa tgcattagtc aatatgactt tatttacttt gttaattgag
     4561 tttatttctt ttgcgacatt atagcttgtc ttaattctat cactaccttg aagtctttta
     4621 atctctattc ctttgccttt tagaacagtt tcagtatatt tatcaataga gttttctcct
     4681 ccaattatat atactttttt tgccttttct aatcttttaa gtgtagcatt tggtatgtta
     4741 tttttcttag tcaaaagaat tggagcgttt atagctcctg caagtccact tgcacttaat
     4801 ccatcagcca ttgtactgtc tgcattaatt aatatagctg tagtatagtt ttgtttgtct
     4861 gcaattatac cagcagtttc gtatttatct gcacctttaa ttgattcaat tttatcaaga
     4921 gcatttacag atatagggca actagcaata aatatagata aagataaccc tattgttaat
     4981 aattttttaa ttttcataat atctccttct cttaaatatt ttcacctaaa ttatataata
     5041 cttttcagga aaattttacc ataaatttaa aaggttatta ttacttaata taatattaat
     5101 gaaaaatatc actcttaaat taatgacttt ggtagaatat ggtgataaaa taaaattaat
     5161 gagagacaat ataaggggga ttatattatg ataatcagta aaaaaataac agcaatatta
     5221 gtatcatttt taattatgat atcaatggtt ggatgtgctc caaaagaaag tgctacagaa
     5281 gtagctaaaa agtaccttga tagtatagaa atcaaagact acgaagtagc atataattta
     5341 ttaagtaaag acagtaagga aagtgtcagt ctagaaaaat ttgaagaata tcaagatact
     5401 ttatttaaac ttcaaaccct aaaagattat aaacttggtg aagaaaataa agtagagaaa
     5461 tacaaatatt ctaataaaga gtacacaaat cttgttaaaa taaaagaaac tgatactata
     5521 aagtatatca ctgaaaataa agaagatact gcaaattttg atagatattt tataatcgaa
     5581 gataatgaat ataaattatt atggcataaa gactttaaaa gagctcttag ctctaattat
     5641 gtcaaaatcg ctaggtcaga aacagaaaaa gatgaagata aggattataa taaagctgct
     5701 attgatatca aaaaagctat agaaattgat gataaaaatc cacttgcata ttatacacaa
     5761 tcatatattt atatagagtt acaacgatat gatgaggctt taaagtcaat tgataaatac
     5821 ttagaatatc tagataaaaa taatgaagct tataaaatag acgctagtga tgcttacaat
     5881 ttaagaggtg ttatttttat gagtaaatca gaatttaata aagctagaga atcttacaat
     5941 aaggcgcttg aacttaaccc ggataatgaa tatgcaaaaa ctaatctaaa tgatattaaa
     6001 ggattataag gcattaatat tatagaaaat aagtatgtca aaagttaaat taacttttga
     6061 catgcttatt ttctatattt taaactctat tgatttacta tataatagtg cataaggagt
     6121 gtgattactt attatgagtg ttgccatata cttaagaaaa tcccgtgctg atgaagaggc
     6181 agaaaaacaa ggcgaatttg aaactttaag tagacataaa tcaaccctac taaaactagc
     6241 aaaagaacaa aacttagatg taattgagat aaaagaagaa ttagtttctg gtgaaagtat
     6301 aatacataga cccaaaatgc ttgaacttct aaaagaagtt gaagaaaata aatatgatgc
     6361 agtcttagta atggacttag acagacttgg tcgtggagac atgaaagacc aaggtatcat
     6421 attagaaaca ttcaaagaaa gtaagaccaa aatcatcaca cctagaaaaa catacgacct
     6481 aacagatgaa tttgatgagg agtattcaga atttgaagca tttatggcaa gaaaagaatt
     6541 aaaacttata tcaagaagga tgcaaagagg tagaataaaa tcagttgaag aagggaattt
     6601 cataggaacc tctgctccat ttggatatga tgctgtaaca actggtagaa aagaaagaat
     6661 acttgtacca aataaagatg ctgatgtagt taggactatc tttgacttat atattaatga
     6721 agatatgggt tgtagtaaaa tatcaaaata cctaaataac ttaggcatta aaactgctac
     6781 aggtgctaat tggtacaatt ctgcaattac aaatataata aaaaataagg catattgtgg
     6841 ctacattcag tggcaaaaaa aggactacaa aaaatctaaa aatccaaata aaataaaaac
     6901 agttaaacta agacctaaag atgaatggat tgaggcaaaa ggaaagcatg aacctttaat
     6961 aagtgaaata acatggaaaa aagctcaaaa tatactcaaa aaaaatggtc atgtcagcta
     7021 tggtaatcaa ataaaaaatc cacttgcagg aatagtgatt tgcaaaaatt gtggtagacc
     7081 attagtatat agaccatatg cagaccacga ctatataatt tgttatcatc ctggttgcaa
     7141 taaaagttct cgatttgaat ttattgaagc tgccatttta aaatctttgg aagacactgt
     7201 aaaaaaatat caattaaaag catctgatat agacttagat aaaaataata aagatagtaa
     7261 tatagaattt caaaaacgag tcttaaaggg cttagaaact gaattaaaag aattaggcaa
     7321 gcaaaaaaat aagttatatg atttactaga acgaggaata tatgatgaag atacatttat
     7381 tgaaaggtcc aacaatataa gttcaagaac tgaagaaatt aaagattcaa ttaagacagt
     7441 aaaaaataga ttgagtactg ttaaaaaaga caattctaaa ataatagaag atattaaaac
     7501 tgtgttatca ctttaccatg attcagattc ccttggaaaa aataaactcc taaaatctgt
     7561 aattgataaa gcagtttatt ataagtctaa agaacaaaaa ctagattctt ttgaattaat
     7621 ggtccatttg aaattacatg aagaccagta ataccaataa catataaaga gtagtgtgta
     7681 atgaaataag tcttttagat atacttggta cagaggctaa ctatgttctt gatgaagttg
     7741 aactaaaagt tcaagttgga aaactttacg aacaactaaa caaaattctt actcctagag
     7801 aaagagaaat tgtccagtta agatatgggt taactcctta tggctataag acacaaagag
     7861 aaattgcaca aaaactcgat atatcaaggt catatgtatc tagaatcgaa aaaaaagctc
     7921 ttaaaaaact agaaaaagag cttgttcaag aaagttaata ctatatttta aaaggatata
     7981 tcgtacatca ttagacaaaa ttttctatct aaaatcaaag ttacgatata tccttaaatt
     8041 ttatagtaca taatttattt tatactttca catatttcct taaatatagg tgttgcagat
     8101 ttatttcctt tttgagttcc ttctactaac acagttatta catacttagg tctttcttca
     8161 ggataaaatc ctgttatcca tccgtggtct atagcttttt tatttaaact actttgagct
     8221 gtaccagttt taactccaca accaccttca atatctttta ggtctttagc tgtaccagtc
     8281 ctagatacag acttcatata ttctttaact tgtgtacaca cataaggaga aataagttct
     8341 tctttttttg aagacttata agtttttatt gtattcatat tattgtccac aagactttta
     8401 tataagtata gtggtttgaa tgttccatta ttagctatta tttgagtcat ttgatttatt
     8461 tgaagtggtg taaattctat attttcttga cctatagcta aatttcttat tgatatgttt
     8521 tttggagctt ctctaatttt ttcttcatca agaccaatat caaccttttc aaataaatgt
     8581 aatttttcag cagatttgag tatcttttct tttccaagtt tcatagctat atctaaaaat
     8641 gcaggattgc atgaattaga aaaagcttgt cttaagtttt ggaaaccatg accatctctt
     8701 ttattacatc gcagtatttc attagtattc ccaactttag tcttacctgt acagttgtaa
     8761 gtatagttct catctataac accattttca agtgcagaaa ataaaactac catcttaaat
     8821 acagaccctg gtggatatgt cgcttttata actctatttt caaattctcc attttttcct
     8881 tttaaacttt ttgatatgtc attttggtca aaattaggtc tagagcacat tgcaagaatt
     8941 tcacctgtag atgcttccga gattattgct gctgtaggat tttcttcttt atttagtatt
     9001 tgttctaatt ttttttgaat gttataatca atagtagttt ttagatgtct atctttatcc
     9061 ttattatcaa cagtttttac acttcccttt agtatgttta gagatttttc atttcctgca
     9121 tctccagctt taaaagcact tatatacttt tcatttgaat ttctaagtac actatccata
     9181 ctcttttcaa ttcctgattg acctgattta tcatcttctt ttatatatcc tattgtatgt
     9241 gataataaac catcttttgc gtacctcata gttttttctt ctaccattat tccatttttc
     9301 tcaagttctt ttttcatact cttatcgata tcgatatttt tagtctgaat ctgtattata
     9361 gggcgtgtca attgctcttg tacagcttta tatatatcta aatcattcat cttagtagct
     9421 ttcttaataa gctccaaaat tttatagtta ttatttaatt tttctttttc aacaattaat
     9481 acctgagatt tacttgtatc tgtaagcttc ttgttgtttc tatcatagat tattcctctt
     9541 ccactattta actcgacttt ctcaacgctt tgtgattcaa cactttgttt gtatttatca
     9601 ccttttaata cttgtatatc aactagccta taaattaaaa cactataaat tattaaaatt
     9661 atagtaaata tacaccagct tctctttccc acttttttca aaaatggagt cttctttttt
     9721 gacatgaaat caccccttag ataatttcat tattgacaat ttctaagggg ctaatactat
     9781 ttttatcaat tttttattat tcttcaacct tttcttctat aacttttcta agcatataat
     9841 ctttaccaac ttttactgat agctttaact tcactatttg tctaggatga ggagctgatt
     9901 ctatagcttc tccttcttca ttatacattt cttcaatttt agtaaacata gtttctttat
     9961 atggtcccat aacttctatt tcatcaccaa cacacatttt atttctttgc tcaacaacaa
    10021 taagaccatc ttcatcttct gtttctctaa ctatacctat aaaatcatag tttctaacat
    10081 atgatgctga ttgataattt tggtcttctg ttgttggttt attcaagtaa aaacctgatg
    10141 taaaatgtct atgactacct ttttttagtt cttccatcca cattggatta aacttccagt
    10201 tttctgggtc tctgtaaaat tcatctattg ccattctgta tgctcttaca acagtagcta
    10261 catagtatgc agtcttcatt ctaccttcta tttttaaact tgttatacca gatttgatta
    10321 actctggtat atactctatc atacataaat cttttgagtt aaagaagaat gtaccccttt
    10381 catcttcgta tattggaaaa tactcaccag gtcttttttc ttccaccaaa tgatattgcc
    10441 atctacaaga ttgagcacaa gaacctttgt ttgcatctct tccagtcata tagttgctta
    10501 ttaaacatct gccagaataa gaaatacaca tagcaccatg cataaacgct tctatatcca
    10561 tatctaaagg tgtttttgct cttatctcat atatttcatc aaaagataat tctcttgcca
    10621 ctacaactct ttttattcca tgctcgtacc agaagtttgc acttagataa ttggttgtat
    10681 ttgcttgtgt acttaaatgt atttccatat tcggaattac ttttttaatg acactaagca
    10741 ctcctgggtc agctattata actgcatcta ttcctatctc ttctagttcc aacaaatagt
    10801 cttctaattg tagaaaatct tcattatgag gtattatatt tactgttacg aatactttct
    10861 tgcctctttc atgtgcaaaa gctactccct ctgccatatc ttccttacta aaattcttcg
    10921 ctgcagacct cattccaaaa atttctccac ctatataaac ggcatctgct ccatatgtta
    10981 tagctatttt caatctctcc aaatcacctg ctggagctaa aagttctact ttatttaatt
    11041 ccatctacat cacctctttt actttcttta taacttagtg ctacaccatc acctattggt
    11101 accaatgatg tgcttaaata gtcacaatta catatataat ctaaatactt tctcattctt
    11161 ttaactatag tcttttttct tcttacaaca aaatcatcat gagcaaccat acctttatac
    11221 aaaatattgt cagatactaa aagtccccca tcttttaatt tatctataac taaatcaaaa
    11281 aatagcttat attgaccttt ggctgcatct ataaatataa ggtcaaattc accttggact
    11341 tgactaagtt tttcttctgc atctccttct agtatagtta tgttattgtc aaatccagct
    11401 aattttatgt tttcttttgc tttttctatc attttttcat ttctctcaac agttattata
    11461 tctgcatctt ttcctataat agttgcaaaa aaaatagaag aataaccaat tgcacatcct
    11521 acttctaaaa ttctctttgg tttttgaacc tttaacaaga cttttaataa atctgaaact
    11581 tctttatgta ttataggaac actattttcc tctgcataaa tttctaaatc ttttaaaaat
    11641 ccttcttttt cttttaatgt cactcttata taatcttcaa ccaaatcatt aactatatta
    11701 ctcataaaaa cactccctaa aataataaat atccaagttt tatattaatt tattattttt
    11761 taaattcata ttgttatata tatttacact ttctttatta taaatctaag aataacaatt
    11821 ttcaataaaa aagcccaaca ttattcttac cctgttagac tttttataat catggtttat
    11881 ttttttgtat tttctttatt tgttgctttt gtatcttttg attgtttatc ccttgcttct
    11941 ttatattctt taacgtattt taaatggtct tgatagtttg tactataatt atttccacca
    12001 tcaatcttag ctacaaaata taaatagtct gttttctctg gatataatgc tgcttcaata
    12061 gattttatac caggatttga tattggtgta ggaggtaatc ccttattttt ataactatta
    12121 tatggtgaat caatttttaa atcatcatat gttacaattt tctttctctc ttcaaatata
    12181 tattgtattg tagcatctga ttgaagaggc atacctacct taagtctatt ataaaacaca
    12241 cttgctatta taggtctgtc tttgtcaaga acagcttctt tctcaattat tgatgccatt
    12301 tccattactt cttgaagagt catatttaat tctttttgtt tctttttaaa cttgtccgtg
    12361 tatttactgt caaatacttt taacatttcg cttaaaatat cttcttctga ttgtttcttt
    12421 gaattaaaat aataagtttc tggatacaag aacccttcta aagaggttat tccatcttcc
    12481 ttcaaatatg aaaatttatc ataaaattct tgaggttttt ttattaaatt ttcaaaagta
    12541 gctttatctc ctaaattttt acttactaac attgctataa tttctttaga tgtagctccc
    12601 tctggaatag tcacttttat accatcctga tatattttac ctgaaactaa aagacttatt
    12661 atatcattgt ttgaatacga ttgattaagt aagtatgttc ctgattttat agacggagct
    12721 ttattgctaa cctttaccaa aagtttaaat aatagttcat tctttattaa tttattttca
    12781 tataatatgt cagatatttt tccaacagat gctccacttg gaacgtcgat aattacatct
    12841 tttttgttat ttttatcata aggacctatt tgtataaaca caaatatacc tgctaagata
    12901 atcaaaataa caattattaa tactgctatt ttcaatctat tttctttaaa g
//
LOCUS       DAMJYX010000094        12906 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJYX010000094 DAMJYX010000000
VERSION     DAMJYX010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.096"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..1171)
                     /locus_tag="PC479_002822"
     CDS             complement(<1..1171)
                     /locus_tag="PC479_002822"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2805351.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKM"
     gene            complement(1194..1859)
                     /locus_tag="PC479_002823"
     CDS             complement(1194..1859)
                     /locus_tag="PC479_002823"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2805352.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(1893..3005)
                     /gene="ribD"
                     /locus_tag="PC479_002824"
     CDS             complement(1893..3005)
                     /gene="ribD"
                     /locus_tag="PC479_002824"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2805353.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(3137..3258)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3447..5930
                     /gene="recQ"
                     /locus_tag="PC479_002825"
     CDS             3447..5930
                     /gene="recQ"
                     /locus_tag="PC479_002825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF2805354.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6019..6130
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6272..7573
                     /gene="thiC"
                     /locus_tag="PC479_002826"
     CDS             6272..7573
                     /gene="thiC"
                     /locus_tag="PC479_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2805355.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            7627..7821
                     /gene="thiS"
                     /locus_tag="PC479_002827"
     CDS             7627..7821
                     /gene="thiS"
                     /locus_tag="PC479_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2805356.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            7814..8617
                     /gene="thiF"
                     /locus_tag="PC479_002828"
     CDS             7814..8617
                     /gene="thiF"
                     /locus_tag="PC479_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2805357.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            8680..9450
                     /locus_tag="PC479_002829"
     CDS             8680..9450
                     /locus_tag="PC479_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2805358.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            9467..10570
                     /gene="thiH"
                     /locus_tag="PC479_002830"
     CDS             9467..10570
                     /gene="thiH"
                     /locus_tag="PC479_002830"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2805359.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            10571..11260
                     /locus_tag="PC479_002831"
     CDS             10571..11260
                     /locus_tag="PC479_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2805360.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            11448..12842
                     /gene="yfcC"
                     /locus_tag="PC479_002832"
     CDS             11448..12842
                     /gene="yfcC"
                     /locus_tag="PC479_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2805361.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         4850 a         1552 c         2207 g         4297 t
ORIGIN      
        1 ccattttatc ttttttagtt tttaaatata gcttattaaa gtcattaatt tcttcattaa
       61 tagggattct attttcgatt tttattccat atttctctaa ttgatttatt ttatctgggt
      121 tattacttaa aagatttata ctttttatgt ttaaattttt taatatttga gcagccatat
      181 agaaatctct catatcttct tcaaatccaa gcattaaatt tgcttcaaca gtatcgtaac
      241 cttcttcttg aagtttataa gccttaattt tatttaaaag accaatacct ctaccttctt
      301 gcttcatata aattaaaact ccacttccat tttccacaat tttcttcata gaagattcta
      361 gctgtaatcc acaatcacat tttagagagt ggaacacatc tcctgttaaa cattcagaat
      421 gaagtctaac tagagtattt tctatattta tatttccata agtaagggca atgtgttctt
      481 catttgaata agtatctcta tatcctatga tttcaaaatt tccatatttt gtcggaagga
      541 aagcactaga tactttttct atagtagttt catttatttt gcggtattct ataaggtctt
      601 ttatagtgat gatttttaaa ttatgctttt ctttgaaaat cattaaatca tcaactcttg
      661 ccattgtacc atcatctttc attatttcac agataagacc aatctcttta aaacctgcaa
      721 gtctagcaag gtctactgag gcttctgtat gtccatccct gacaagaacc ccaccagttt
      781 ttgctattaa aggaaagatg tgaccaggcg ttttgaaatc tttacttgta gaattttcat
      841 cacagaataa ttttacagtt tcagctcttt catatgcaga tataccagtt agagtatctg
      901 ctccatctat agtaacagta aaggctgttt gaaatgtatc tgtattttct ctaaccataa
      961 gaggtatatt aagactttta aatttttttt cagtggcagg tagacaaatt agacctctac
     1021 catatgtagc cataaaattt attgattcag gagtagccat ttcagctgcc atcaataaat
     1081 ctccttcatt ctctctactt tcatcatcaa ccacgattac cattttccca ttttttatat
     1141 cttcaatagc ttcttcaata gtattaaaca tgtaattcac tcctttatta tttttaaaaa
     1201 ccatttttaa ataaaaaatc tttatctata tttgatttat ggttattact attagaattt
     1261 aattccttgt agttattagc cataaaatta ttaacatact ttccaattac atcgctttct
     1321 aaattaacga aatctccaac attctttgtc aagagaatag tgttaatttt agtatgaggt
     1381 ataatagaaa ccttaaatat ttcatcatct aaatatgata tagttaaact tacaccatca
     1441 atagcaacag aacctttgag tatcattgag gataaaagtt ttttatcagg tcgtatactt
     1501 actaaaactg cattttcatc tttttcaaat ttgcagatag ttcctttacc atcaacatga
     1561 cctgttacta tatgaccacc aaatcttgaa gaaagactca tagctctttc aagatttacc
     1621 ttgtcatttg catttaataa acctaaattt gtggaacgaa ttgtctccat cataacatcg
     1681 gctgtaaatt cattttttcc gaggtttgaa acagttagac aaactccatt tacggctata
     1741 ctatctccaa gatttgtgcc atctaatatt ttatttgata gtatggttac ttttccactc
     1801 ttaccattag cagtaatttt tctaagtatt ccaacttctt ctactatgcc agtaaacatc
     1861 ctaacctcct atatgaatgt attaacaaaa atttaattta aaacatctga ttctataaga
     1921 atatcgccat ctatcaaagt tacattattg attgttaact gtgttgaatc tgacaaatga
     1981 tttattcctt ctccagaaac gaatgtaggt gcatctttac cacctataat cttgggtgat
     2041 agaaataatt tgaccttatc aactaactta tttctaaaga gactgtcatt gagagtagca
     2101 ccaccttcaa ctagaacaga atcaatgttt aactctccta atttatacat tacaaactcc
     2161 aaatctacta agttgttttt tgaaggagaa attattgttt caacattatt ttcttttaac
     2221 atagataatt ttatactttc tttgcaacat gtgaaaacta tagttttact ggttttatct
     2281 ttaaccaaat tactattaag tggcaacttt aggtgtgtat ctattactat tcttgtagga
     2341 tgagacactt tttcttgatt gagcctacaa gttagtaaag gattatcgtt caaaactgta
     2401 tttatgccaa ccattatact ttggtattta tttctgtatt tatgagttag ataccttgat
     2461 gattcattag atatccattt tgattctagt gattttgtag caattttacc atctagactt
     2521 actgctgatt ttacaataca aagaggtctt ttgtttttta tatgataaaa aaatgcctca
     2581 tttaaatctc ttgcttcatc ttctaatatt cccacagtaa catcaatatt gttatctttt
     2641 aattttttta ctccattacc acaaacaagt ggatttgggt ctaatgtaga tattacaact
     2701 ctcttaatct tattttgtat aattttatct acacaaggag gcgttttccc ataatgagaa
     2761 catggttcta agtttacata aagagtagag tcttttagac tttgtttagc actgtttata
     2821 gcatttactt cagcatgatt agagccaaat ttctcatgat atcctttgcc tattatatta
     2881 ctagctttaa caattacaca cccaactaat ggatttggat ttacaaagcc ttctccattt
     2941 ttagccaatt caatagcttt tttcatataa taaatatctt tttctttttg attcacaaaa
     3001 ttcacaatat gtacacctcc tgtttaattg gataattttt aaacaaaaaa ccctgagaat
     3061 taactcaggg tttagaataa tctacaataa atgtttaata aatgtagaac aaaattgctc
     3121 tacaaaaaca ctcattaaat atcttcttcc atccagactt tactgtcggc tttggaattt
     3181 aaccaaatca accaagtttt accttggctc gcgggctata ccgccgattg ggaatttcac
     3241 cctaccccga agattaagta ttaaatttat atttagaatg tatcataaat tattttactt
     3301 tgcaaactat ttttttaaaa aatatatgtt aatatataat atagaatgtc tataaacttt
     3361 gaaataccac gattgtgaat ataaaaataa aaatttgaac ataatcagga aagaagtcta
     3421 attaatttaa attaggagag taatatatga ataaaaaact tttagaaata ctatcaaaat
     3481 actatggata tacaagtttt agaaaaggtc aagaatctat aataaactct atcttaagcg
     3541 aaaaagatgt gttagctatt atgccaacag gagggggaaa atcaatatgt tatcaaattc
     3601 cagctttgat tttagatgga atgacaatag taatttctcc acttatatca cttatgaaag
     3661 accaagtaga tgccttaaag actatgggaa ttagtgctgc ttttataaat agttcattaa
     3721 gtagcaaaga atttaatgaa atattaaata atattagaaa aaataattat aaaatactct
     3781 atattgctcc agagagatta gatgcacaag agtttattga gctaataaat aataacaatg
     3841 taagtcaagt tgcgatagat gaagcacatt gtgtatctca atgggggcat gattttaggt
     3901 tgagttatag aaggatttct gattttataa aaaatttgcc taaaagacct atagttacag
     3961 catttactgc aacagcttca gaagaggtaa gaactgatat aataaattta ctgtgtttag
     4021 aaaatccaga ttattatata actggatttg atagagagaa tttatcaata aatatagtaa
     4081 aatcatctag taaaaataag tatattttag attatattca aaatcataaa aatgagtctg
     4141 gaattatata tgttgcaact agaaaggaag ttgaaaatat atataatggt ttatctaaga
     4201 gaaatatatc tgtgtcaaaa tatcatgcag gtttatctaa aaatgaaaga agtaaaaatc
     4261 aagaagattt tataaatgat aatgtggata taatggtagc aacaaatgca tttggaatgg
     4321 gtattgataa acctaatata agatgggtta tacattacaa tatgcctcaa agtatagaaa
     4381 actactatca agaaataggt agagctggta gggatggaga aaaaagtgag tgtgtacttt
     4441 tattttctcc aggagatgta catatacaaa aatatttaat tgatattgga gttgaaaatc
     4501 ctgaaagaaa attatttcag cataagaagc ttcaatacat gattgactta gtttacagta
     4561 attcatgcta tagaaagaca atattgagtt attttggaga aaattatata gacaattgca
     4621 ataattgtag caattgttta gtagagggtg aaatagtaga taaaactata gatgcacaaa
     4681 aagtcatttc atgtgtagct agaatgaaaa gaagttatgg tgttactact attgtagatg
     4741 tgcttagagg ttctaagaat aaaaagattt tacagcttgg attaaatact ttgtctacat
     4801 ataacattat gagagattat tctagtgaag acttaaagaa ttttgtaaat acattggttt
     4861 ctcatggttt tttagattta gttgagactc taggcaatgg aaatagaggt agttttccta
     4921 ctataagatt aaatgagatg tctatgaaag ttttaaaagg tgaagtgaaa gtagagttta
     4981 aagaaattgt tatgacaaaa tcattggaag tagaggatga gttatataat gctttaagag
     5041 aacttagaca ttcaattgca agtgaagaaa ggatagctcc atatatggta tttggagatg
     5101 gtacacttag agccatgtct agtagttatc caattaataa agaagaaatg ctagatatct
     5161 ctggagtagg agaaattaaa tatcaaaaat atggtagaga ttttgaagca gtaattaaag
     5221 aatatgtcga aaaaaataat ataaataaaa aaatagatga aaaaaatgtc gattcaaaga
     5281 acgttaatag tgatttctta gaggttaata cagataaagt tttatatgaa aaactgttag
     5341 tcattagaga agaatatgca acaaaggaaa aattacctgc atatatgatt ttagccaata
     5401 aggctttgaa agaaataagt ggaagatatc cacttgatga agaacaatta aaagatattt
     5461 ctggcatagg tggagtaaaa tttgaaaaat atggagctag tattttggca gaggttaata
     5521 aatatgtaaa agaaaataat attaatgtta tttgggagaa aaagggcaaa aggaagttaa
     5581 tattagatgg agaaagtaga aaaaataatg aaatagcatt agatttgcta aatcaaggaa
     5641 aagatataca aagtatcagt caagaaatag aagtttcact gtcaaccatt attggatatg
     5701 tgcatgacta tgtaaaggat ggacatagta tatcatttga attaggacta gaaaattttt
     5761 ataatgaaga taaaaagaag atgattttag aagtttgcga aaaagttggc tatgataatt
     5821 taaataacat aaaaagaaaa cttccagatt caataaagta tgaaaatata agagctgtga
     5881 tacttgactc ttatcttgaa aacttaaaaa ataatattat agcagagtaa tttttaactt
     5941 aataaaaaat aaaatttgta ttattgtgtt gaactaataa ttttgctgtg ttatactgaa
     6001 tttgtaaaaa taatgaaaaa ttagagctag gggcgctaga attggctagc tgagagataa
     6061 agccgtaact tacaaaccct aataacctga tcaagataat gcttgcgaag ggaagctttt
     6121 taatataatt ttattatata aataggcaaa ttattcaatg ttaggagaaa tttgtctaag
     6181 tgtatatacg attataagag tgctttccaa acgaggaaaa gcactttttt tattttcaaa
     6241 aactataact ttaatcttgg aggattgtaa tatgaactat acaacacaaa tggatgctgc
     6301 tagaaaaggc ataataacta aagaaatgga aatcgtttct caaaaagaac aggtagatgt
     6361 aaatgaatta agagaattaa tagcaaatgg acaagttgtt ataccagcta ataaaaatca
     6421 taaatcttta agtgcagaag gtgtaggtaa aaatttaaga acaaaaataa atgtaaatct
     6481 aggaatttca agagattgta aggatataga aaaagaactt gaaaaagtta gagtagcaat
     6541 agacatgaaa gctgaagcta taatggattt aagtaattat ggcaaaacta gagaatttag
     6601 agaaaaggtt gttgaaatgt cacctgcaat gataggttca gtacctatgt atgatgcagt
     6661 aggatatttg gaaaaagaat taaaagatat aacagaagaa gagtttttaa atgtaataag
     6721 acaacatgct attgatggag tagactttat tactatacat gcaggtctta caagaagtgt
     6781 ttgccaaaaa ataaaaaatc atgagagatt gactcatata gtatctcgtg gaggttcttt
     6841 attgtttgca tggatggaat taaataataa ggaaaatcct atttatacta attttgataa
     6901 aatattggat atatgtgaag aatatgatgt tacattgagt ttaggagatg cttgtagacc
     6961 aggttgtata aaagattcta ctgatggtgt tcaaattcaa gaacttgtag tattaggtga
     7021 acttactaaa agagcatggg aaagaaatgt acaagtaatg atagaaggtc ctggacacat
     7081 ggctattgat gaaatagaag caaatgtagt attagaaaag agattatgcc atggagcacc
     7141 tttttatgtt cttggaccat tggttacaga tatagcacca ggttatgacc atataacaag
     7201 tgcaatagga ggagctttag catgtgccaa aggagtagat tttttatgtt atgtaactcc
     7261 agcagaacat ttaagattgc ctaatttaga tgatatgaaa gagggtataa tagctgcaaa
     7321 gatagctgct catgcaggag atatagctaa aaatgttaaa ggtgctcgtg aatgggataa
     7381 taagatgagt aaagctagag ctgatttaga ctggtgtgag atgtttagac ttgcaataga
     7441 ccctgaaaaa gctaagagat acagagatga atcaactcct actcatgagg atagttgtac
     7501 tatgtgtgga aaaatgtgct caatgagaac agttaagaaa atattaaaca atgaggaact
     7561 taatctaata taatttttgg ctagtatgac tggaataaaa ataagctgaa aagaggaggt
     7621 gatttagtga aggttaatgg gaaggaaatt gaatttgaaa aagatttgac tgttatagat
     7681 ttattaaata aatataattt aaaaagtgat agagttgtag ttgaagtgaa tttagagata
     7741 attgaagaat ctaactacaa tacatatgta ttaaaagacg aggacattgt agagcttatt
     7801 agctttattg gaggtggtta aaataaaaat atttgtaaat gaattattaa cagatgttga
     7861 ggaaaataca actgcttata aagttagaga tagctataac aataaatgtg atgtattggt
     7921 gttaaatgga tatccaatta aaacagatat gaccctttgt gaaaatgata aattaacact
     7981 tatacaaaaa ggagttaagc catcacttga tgaattggaa agcttgttga ttgctagaca
     8041 tactccaaat atacataata aattaaaaaa agggaaagtt gctatattgg gattgggggg
     8101 acttggttcc aatatagcta tctctttagc tagaatagga gtgggagaac ttttacttat
     8161 agattatgat gttgtagaac catctaattt aaatagacag cagtatttta tagatgatat
     8221 aggaaaacta aaaactgatg ccatgataga aaatataaaa aaaataaatc catttataaa
     8281 attaaataaa cgagatattt ttttaaacaa gaacaatatg gatactatta aggattcaga
     8341 tttaataatt gaggcctttg atgatgcttc atgtaaagca caggtatgta attatgtatt
     8401 aataaattta aaagataaat atttaatagc atcatctggt atggcaggtt actatgattc
     8461 aaatataata actactaaga agataaaaga caggttttat atatgtgggg attttgtgaa
     8521 tgaagctaaa tttggtgaag ggcttatggc tccaagggtt gccatctgcg caaatcatat
     8581 ggcaaatttg gcaacgcaaa tacttataga tatgtaaata ttaaaattag atataaaaaa
     8641 gattgaatat taaaaaaatt taacgtggag ggaaataata tggataaatt ggtacttggt
     8701 ggacatgaat ttaatagcag attattggtt ggaacaggta aatatggttc aaataatata
     8761 ttgcctgagg taataaaaga aagtggaagt gaaataataa caatggcact tagaagagtg
     8821 gatttagaca ataaacaaga aaacatatta acgtatattc caaaagagat gacaatactt
     8881 ccaaatacat caggagctac taatgcagaa gaagctgtaa gaatagctag aatatctaga
     8941 aaaatgggat gtggagattt tataaaaata gaagtaatat cagatactag atatttactt
     9001 cctgataatg aagaaactat aaaagctaca aagatacttg ctgatgaggg gtttatagtt
     9061 cttccatata tgacaccaga cctttatgct ggaagaagac ttatagaagc taatgcagcg
     9121 gcagttatgc cacttggagc tccaattggt tcaaatagag gtcttcaaat gaaagaaatg
     9181 ataagaataa tgatagatga gttggatata ccaataatag tagatgcagg tattggaaaa
     9241 ccatctcaag ctatggaggc tatggaaatg ggagctgatg cagtacttgt caacactgct
     9301 atagcttcag caggagaccc tgtacaaatg gctagagcat ttaaattagc tgtagaaggt
     9361 ggaagagaag catatattgc aaaaacagga aatgtgtcag aatttgcaaa tgcatcttct
     9421 cctttaacag gttttttagg caatttatag aaagtggtga ttggtgatga gtttttatga
     9481 tgtaatagaa aaatataggg attttgattt tgatggatat ttaaataatg ttactgataa
     9541 tgatgtttta agaagtttat caaaagacaa gcttgaagat tttgatatat taaatttgct
     9601 ttctaaaact gcagtaaaac atttagaaga tatggcacaa aaagcacata aattaagtgt
     9661 tcaatacttt ggtaaaacag tatgtttata tacaccaatg tatatagcaa attattgtgt
     9721 taatcaatgt gtttattgta gctataatat aaagagtgga ataaaaagaa aaaaattaac
     9781 tatggatgaa attagagaag agggagaagt aatttcaaaa gaaggtttca aacatctact
     9841 tgttttgact ggagaaagta gttttcactc aagtgtagaa tatataggtg aggctataga
     9901 aatattaaga gagaaatttc cctctatagg tatagaagtt tatccaatgg aagtagaaga
     9961 atataagtac attgtagata aaggtgttga aggattaaca gtatatcaag aaacttatga
    10021 cgaggaaata tataagagag tacatataaa aggcccaaaa tcaaattata aatttagact
    10081 agatgctcct gaaagagggg caaaagcagg tatgaggact ttatcaatag gtgcactttt
    10141 agggctaaat gattttagaa aagaaacttt ttttacaata ttgcatggga aatacttaaa
    10201 aacgaaatat cctcatattg agctttcata ttcaacacca agaatgagac cttttaaagg
    10261 gtgttttgag gaattggtag atataagtga tactgattta gttcaagcta tggtatgtat
    10321 gagattattt gaccctcatg cagctataaa tatatccact agagaaaatt tagaaatgag
    10381 aagtcatata atacctttag gagtaactaa attgagtgca ggtgtatcta ctgatgtagg
    10441 aggtcattct caagatgaac atgatactgc tcaatttaag ataaatgatg aaagtactgt
    10501 taaagatgtg gagaaaatgc taaactcaat tggttatcag catgtgttta aagattggga
    10561 aagattttaa atgtatctga taacaaacag aaaattatgt agtgaggaac gatatttaga
    10621 agttattaaa gagtctatat tgagtggtgt agaaaatata ataataagag aaaaggattt
    10681 agaatatcaa gagctaagaa aattatatat gaaaataaaa actaaaatca attgtataga
    10741 ttttcaagag caaatatcag atgaaagttt gaaaactaat ataaatcaaa aagagtttag
    10801 aaataaattc aaggttaatt ttataataaa tagtaacatt gaattttttg aaaaagtgga
    10861 ttgtcaaggt atccatcttc cttttaaatt attcttgaat ttaattgaaa ataagtataa
    10921 ttttaatgaa aataaaatac taggattatc tttacataaa gtagaggaag tagattattt
    10981 agaaaaatta ataagaaatc aaaatataaa aatagattac ataacattat cacatatata
    11041 tgaaactaaa tgtaaagaag gattaaatcc aaaaggaatt gagcttttaa aagaagctaa
    11101 aaagattact gatattaaaa taattgcact tgggggaatt ttaccatcta atgtcaagga
    11161 aacattaaaa tactgtgatg attttgctat aatgtcaact ataatgaggt ctaaagatat
    11221 taaaaaaacc atatcaaatt ataatgaaaa gttgaattag taaatatagt atattgaatt
    11281 tttaacttaa ataaaaataa aaaagttatc ttttaaattt taaaaggtag cttttttatt
    11341 ttatatcttt gctaataaaa aattgtaaaa agggaaaact ttagtcagag agattaaaca
    11401 atatcctaga aagatttaaa atttagtaaa gtatggagga cattataatg actgacagta
    11461 aaaagaaacg atttattata ccacacactt ttacaataat atttttacta atagtcttga
    11521 tggcaatatt gacttggata gtaccaagtg gtgaatttag tagagaagat gtagaaggta
    11581 gaatggtagt tgtaccaggg acttatcaaa gtgtagaaag taatcctcaa ggatttgcag
    11641 atgtttttaa agcaccaata gaaggcttta tcgattcagc tgaagttgta ggttttgttt
    11701 taatagttgg aggtgctttt ggtattgtaa ataggactgg agctatagaa gcaggaatag
    11761 cttcggttat ctctaagttt aagggaaaag aacttcttat aattccaata tcaatgattt
    11821 tatttggact tggaggtaca acatttggga tggcagaaga gacattacca ttttatatga
    11881 tatttgttcc actcatgaca tccttgggat atgattctct aacagcaata gcaattgtat
    11941 ttataggagc agcagcagga gcagccgctt caacaataaa tccattttca gttggtattg
    12001 cacaggcact agctcaactt gctcctggtt caggagtaac ttacagagta gttattttta
    12061 tagcatatat actaataagt atagcatttg ttatgtacta tgctaagaag gtaaaggaaa
    12121 atcctgaaag ttcaatagta tatgaaatag ataaaaataa taaatcaata cttaatcaaa
    12181 cttcaacaga aataaaagaa tttactttta aagaagtatc tgttcttgct atatttggaa
    12241 tagggatggc aataatgatt tggggtgttt taagtcaagg atggtacatt ccagaaatat
    12301 caatgtgttt tgctgcaata ggtgtgcttt ctggattggt tggtagatta tctcaaattg
    12361 aaatctcaga aagttttata gatggagcaa aagatttagc atctgcagct cttgctatag
    12421 ctttggctcg tggaatcgta ataattgctc aaaatggatt tataatagat actatattaa
    12481 attccgcagc agcctttcta ggtgggcttc caaaggttat atttgtatat ttatcatttt
    12541 tcttggaagc tgcattggca tttttaattc catcatcttc aggtcttgct tcacttacag
    12601 tacctgtact tgctcctctt ggtgatttgg ttaatatatc ggctcaagtg atagtaaccg
    12661 cttatcaatt tggtgtgggt cttacaaact ttataactcc aacttcagga gtactgatgg
    12721 gagcacttgc agtagcccat attccatttt caaaatgggt aagatttata atgcctatct
    12781 tgttgatttt gacagctgta tctctagtct ttttaacaat aggtatttat atagggtttt
    12841 aattaagtgt agttttattt aagatattta gtaatggtat aaaatgataa tatgttttca
    12901 ataaat
//
LOCUS       DAMJYX010000095        12819 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJYX010000095 DAMJYX010000000
VERSION     DAMJYX010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.097"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     regulatory      46..148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            242..1618
                     /locus_tag="PC479_002833"
     CDS             242..1618
                     /locus_tag="PC479_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2805362.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1732..2877)
                     /locus_tag="PC479_002834"
     CDS             complement(1732..2877)
                     /locus_tag="PC479_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2805363.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3130..3837
                     /locus_tag="PC479_002835"
     CDS             3130..3837
                     /locus_tag="PC479_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805364.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3884..5479
                     /locus_tag="PC479_002836"
     CDS             3884..5479
                     /locus_tag="PC479_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805365.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5582..6664
                     /locus_tag="PC479_002837"
     CDS             5582..6664
                     /locus_tag="PC479_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2805366.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6813..6974
                     /locus_tag="PC479_002838"
     CDS             6813..6974
                     /locus_tag="PC479_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2805367.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7315..8583
                     /locus_tag="PC479_002839"
     CDS             7315..8583
                     /locus_tag="PC479_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2805368.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8778..9566
                     /locus_tag="PC479_002840"
     CDS             8778..9566
                     /locus_tag="PC479_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2805369.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9566..10015
                     /locus_tag="PC479_002841"
     CDS             9566..10015
                     /locus_tag="PC479_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2805370.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10019..12283
                     /locus_tag="PC479_002842"
     CDS             10019..12283
                     /locus_tag="PC479_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2805371.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12423..12734
                     /locus_tag="PC479_002843"
     CDS             12423..12734
                     /locus_tag="PC479_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805372.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4694 a         1395 c         2188 g         4542 t
ORIGIN      
        1 tgacaaaatt aattatataa attaaaataa tattcgtaaa gaaaaattca tgttttgtac
       61 tgtataacat ataattttga caatatgggt cataagtttc taccggaata ccgtaaatat
      121 tctgactatg tatactatta tttagttaat taaatattta attaactgaa ttatgaagga
      181 tacggctcag cttatcaact actgtgtcgt ttttttgtaa aatttaatag gaggtgttgt
      241 tatgcaaaca acaaacagta gtactatgca gaaacttttt ccaattttga ctaataaaaa
      301 tgtcgacatg aagaaggaaa taattgcagg agtaactact tttttaacta tggcttatat
      361 aatagctgta aatccaaata ttttatctga gacaggcatg cctgcaggtg cacttgttac
      421 aggtacttgt ttagcagcag catttggatg tattttaatg ggtgtagttg ctaaccttcc
      481 ttttgcttta gcatcaggta tgggtcttaa tgcatttttt gcatatacag tagttttaca
      541 aatgggtgtt ccttgggaag tagctcttac agcagtattt gtagaaggta ttatttttat
      601 agtattatca gtaagtggag ttcgtgaggc tgttgtaaat gcgataccaa aaaatatgaa
      661 attagctgtt acaggtggta taggaatatt tattgcactt ataggtctag ttaacagtgg
      721 aattgttatt ggagaccaag ccactcttat caaaatgggt agatttacac cagcagtaat
      781 aataacttgt gtaggattaa taattattgc tgtattagat aaaaaaagag ttaaaggttc
      841 tatacttttt ggaatagtag tgagttcatt acttgcttgg gcatttgctt ttataaatcc
      901 agagtatgca caaaaattag gtatatattt acctggtgga ttatttaaat tcgaaagttt
      961 agcaccaata gcaggtaaaa tagatttagg atatgtacta catccaacaa atataggtgg
     1021 ttttttagta gtagtttgta catttttatt tgttgatttt tttgatacag taggtacatt
     1081 agttggtgtt tgttctaaag caaacatgtt agatgaaaaa gggaatgttc ctaatgtagg
     1141 tagagcctta cttacagatg cggttgcaac tacagttggt gcaggacttg gagtatctac
     1201 agttacaact tatgttgaga gttcaacagg agttatcgca ggtggaagaa ctggatggac
     1261 agcaataaca gttggaattt tattcttagc tgcaatgttt ttctctcctg tatttatagc
     1321 aattccatca tgtgctacag caccagcttt aatatatgtt ggatatttaa tgcttggtac
     1381 agttaaggat atagagtttg ataatataac tgaaggtgtt ccagcttttg ttactattgc
     1441 ttgtatggca ttaacatata gtataggtga tggtttaact ttaggtatat taacttatgt
     1501 tttcgtaaat atattttata atatatttgg agctaaaaag gcagaagaca aaaaacatgt
     1561 ttctgtagta atgattgttc tggcaatatt atttataata aaactttggc tactataata
     1621 ggtattattt aaaattttaa tttaatataa aataaagggc tgtaatttat tatcaattgt
     1681 gatgataaac tacagctctt ttgtatttgt tgtatttttg tatttattgt attatttaat
     1741 aatgtctatt agttctccat tttgtaaagt tttaggtgta attactatat tgaactcttt
     1801 ttgcaaatct tttataatat ctaattcttt ttttgttatt attgagattg tgttaccact
     1861 tcgatttcct ctagcagttc gaccacatct atgcaggtat tcatttttac tttttggaaa
     1921 atccaagtta aaaacatgac ttacatccac aatatctagt ccacgagcag ataaatcagt
     1981 tgtaattaaa atctttgctt taccaagttt aaatttattt attgcattct ttctatcttc
     2041 tttttccata tttccaaaaa ttcctattgc tttgtaatta tgataattta gttttgatac
     2101 taggacttct atattttttt cattatttac aaatacaata gctttttttg gatttgtagc
     2161 tgctaatgcc tttctaagaa aagtaaattt atcccttatc tcacctaata aatatgagtg
     2221 attgatgtta ggatttattt gtgatttttc ctttgttttt attatttcaa actccttcat
     2281 aagtttatca caaattttta tagtgctatc agtaagactt gcagagcatc ctataatttg
     2341 tctatctctt aacgttgttc ttattatgtc ttctatacaa gttatatttt ttccattcaa
     2401 tagattgtcc acttcatcta agataatagt ttttatatta tgtgatttta gtttcttttg
     2461 ttttatcaag tctaaaactc ttccacaaga gccaattact atgtgtggtt ttactgcttt
     2521 tatatttttt atttgttttt gtatattgac ctccccaatt aaagctaaag aagtaacgga
     2581 gagttctgca tttttagcca ataattcaac ctggtttgta atctgcatta ctaattcatg
     2641 cgtaggtgca agtataagag cttgtgtttc tctttttgat gtatcaattt tttcaaatat
     2701 aggtaaaagg tatgctagtg tttttcctgt tccagtctgt gaattaatca ataaatcctt
     2761 attttgaatt atattcccta taacaagtga ctgaacttct gttggagaag ttatgtcttg
     2821 ttttttgagt ccatctatta gagtggaact tatttttaat tgttcaaatg tattcatatt
     2881 ttactcctta tattttaata attatagtca actatttttt attatacatt gtttattcaa
     2941 atcgaactat atattctttt ataattatgt gtaatttgaa agtaaatatc aattatattt
     3001 atacctttat atttctgata cctttgcatt ttatagattt ctaaaataaa caaataaaag
     3061 ttgtaattgt ttgatatttg ttatatatta tatataacaa atattaaaac tataatatgg
     3121 agggaaatta tgttaaaagt aatcaatgta tctaaatcat ataaaaataa aaaagtagta
     3181 agtagtgtct catttgatgt aaatgatgga gagctttttg ggtttatagg acataatgga
     3241 gcaggtaaaa ctactactat taaagcaatt gttggaatac acgattttga agagggagag
     3301 gtactaataa attctaaatc tataaaaaaa cagcctgttg aatgtaaaaa ggaaatggca
     3361 tatatacctg ataatccaga tttatacgag tcattaactg gtatacaata ccttaatttt
     3421 atagctgaca tatttgaagt tgccaaagat aaaagagaag agttaataaa atattattca
     3481 aataagtttg aactcaataa agcattagga gatttaatat cttcatattc acatggtatg
     3541 aaacaaaaat tagctataat ttcggctttg atacacagcc caaaaatctt aattcttgat
     3601 gaaccttttg taggtcttga cccaaaagct tcctttatat tgaaggaaat tatgagggag
     3661 ttttgtgaca aaggtggttg tatatttttc tcaacacacg ttttagaagt tgctgaaaaa
     3721 atttgtgata agattgccat aataaaagat ggaaatataa ttgcttatgg aactacagaa
     3781 gaagttaagg gaagtaattc acttgaaagt atctttatgg aattaataga gaaataaaat
     3841 gttttttata caatatagtg tctaaagtga ggaatgataa aatatgagca atctaagtat
     3901 attaataaaa actaatataa taaatgaatt taaattaaat gcgttaaaaa aagctgatgg
     3961 tagagaaaaa caaaaaataa tgggaatgat gctttcagtt gttataatag cgggtctatt
     4021 aattttttat gtaacaagct taagtctctc tttagttgat atcttaaagc aaataaatca
     4081 actggaaatg ctactgatat ttggttttgg attatcaaca ttaacaacta ttgttacatc
     4141 attatataaa acgtcatcat atctatttca agcaaaagat ttagaccttt tgactagttt
     4201 acctataaaa gaatctactg tactagcaag taagatatta atgttggttg gagcaaacta
     4261 tttattttct tcaatatgta tgattatacc tgcagtggtt tactttttaa attctgatgt
     4321 aagtataact tatttccctt atttgatatt gctattttta gctttaccat tattaccgat
     4381 agttgcttca tcaataattt ttctatttat agggcagata tcatcaagaa taaaatacaa
     4441 aaatttagta ttaatcatag gtagcattat attgatgttg ctttataccc tacttatgtc
     4501 aagacttaat tctatgtcag ttgaaatgct aaagaatagt gtgtctattt cagagacaat
     4561 tagaaaagta tatcctccta tatattattt tgttgatgct cttaaaacag gaagtatagt
     4621 ttcattttta gcatttatag ccatctcaat aataccattt tttatgtttg taactctatt
     4681 tgcaaaaaaa tttaaagata taaatgctaa gatgagtgaa ggatataagt caaaaaatta
     4741 tgaatttaag ggtcaaaaaa cttcaagacc ttcaaaagct ttattaaaaa aagaatttaa
     4801 gagatatact tcttcgttta tatacttttt aaattcatct gcaggtttgt tatttctaat
     4861 ggtaggtaca attttaatat tattatttgg agcagataaa gtgggtgagt tactgaaatt
     4921 gaatatagat tttaatctta taaaaataca attattggga gtattcttct ttataatagt
     4981 aatgaattgt acaacatact gttcaatttc tcttgaagga aaaaacttat ggatactgaa
     5041 atcttctcca atagatgaaa gagaaatatt ctggtcaaaa atattgatga attttttatt
     5101 gaatgcacca ctatctattt tatgtctaat attagtatct ataagattag actttgaaat
     5161 tcagtttatt attgtatcaa tctgtttgat tgtaagtttt tcaatgtttg tagcattgat
     5221 gggattatta tcaaatttat tatacccaaa ccttgattgg aaaaatgagg tggctgtagt
     5281 aaaaagaagt gcaagtatga tagttacaat gttagtatca ttagcttatg taattatact
     5341 tggaggtata tatgtattac ttggaatttc gtttttaaat atatatatta ttttggcaag
     5401 tatacttact ttgatattag attttatgat atggagaatt atttctacta aaggagtaag
     5461 aatatttaaa aatttatagg tgtatttatg tcaaataaag acttatcttt tttagtcttg
     5521 caaataatag gttaagtagt atactatgta tagtgatatg aaaaaattta ggagaaaata
     5581 aatgagaaaa ttatccaaaa gagaaaaaga ttttattcaa tatggatttt tattattttt
     5641 gatatgtatt actacttatt tagtattaac tactttagat ataaaactaa tacctaaaat
     5701 tataaggcta gtaaataaga cttatatatt tcttggcata ttgacaatta tagtatatat
     5761 gataattgaa tctattgtaa ctcatttgat aataaatgca gttcaaaaaa caaaagtaaa
     5821 atttattggt attaaaatgg ctacaatggg attttactat aacttagtta ctccatttgc
     5881 atctgggagt caaccaatgc aaatatatgc acttacaaaa tatgatgtaa gccttagtaa
     5941 atcagtggct atagttacta ataaaactgt agtatttcaa agtacagtta ctgtattttg
     6001 tggaatttta attttgctaa ataagtcatt actgatggag caagtacatt ctgtgaaagt
     6061 cttgataact tttggaatga ttatgaatgt aagtatgttg ttaggtggac tactaatagt
     6121 atttagtcca aaacaagtaa aaatgatagc agaagttttg attaattttt tatctaaatt
     6181 taaaacattt aaatttttag attcaaaacg tgatagaata aatcattata ttgatgatta
     6241 taattattct ataaaaatat ttattaaaaa tgtaagaatc ttagttttaa gtatattact
     6301 tactgtgtta caattaatcg catatttcag tatagtttat tgtgtgtata aagcctcaaa
     6361 tttaagagga gtttcatatg tacatataat gacattacag gtctttttat atatggctat
     6421 atcaccaatt ccaacaccag gaaatgttgg agctaatgag gttacatttt ttactatgtt
     6481 ctccaatata attccaaaag aattattggg gtactctgta ctcttatata gtgggtttgt
     6541 atactatttt atattaatag tttcaggttt atttacattt ggaacgcatt atacattaaa
     6601 ttcttggaaa aaggaaaata aagtaaattc taatttagat aaggttattg aaaaattaaa
     6661 ttaataaaat aattagggaa attctatata taaatatggt gttgaataag ctatattata
     6721 ttgagatatt tccctttttt gattaaaatt atagaaattt gaaataataa gtaataaaca
     6781 gaaaaagtga tattgttttg aaaggagaaa ctatgaaaca agataaacaa aatgaaaaac
     6841 aaagtgagat ttctaagata aaacaaagca atgataataa acttgaaata gctcaagaat
     6901 tacatgtaag taaaaatata gggaatatgc ttggatatag aatagataag aaaaaaatga
     6961 atagaccaag ttaatgaaaa tgttgaaaat ggtacatttt aaaaaatata tataatgaaa
     7021 tgttttattg ttaaatttaa acttatttta atgggggaaa aagtcgaaaa atatctaaaa
     7081 agatatggat ataaaaaata ttaatatata tatactataa acttgacaaa atcaaccttt
     7141 taataactag caaaaaatag caaaattata taagcgttat ttaattgtca aaaatgaaag
     7201 ctaaattgca tgtcctaaaa aaaatgattg ataacattta tttgtgtgct ataattatat
     7261 agtaaaacaa taagtatatt gcataatgta tgcaaatcgg gaggagagac ggacatggaa
     7321 gcatctatac acacaattgc cagcaataat ttattgataa tttttgctat tgtaagtata
     7381 actggaataa tttgtagtaa gcttagtgaa ataataaagg ttccagatgt agtgttatat
     7441 ttacttgtag gtattttaat aggaccatca tttttaaaat ttatagatat tcgtggattt
     7501 caaatagaaa atcaactaat acttacattt ggttctgcat ttattttata tttaggtgga
     7561 aaagaaataa gtttaaaagt attgagaaat gtgaagatat ctgtattctt attagctact
     7621 ttaggagttg tgatatctgc ttttataatg caacaagtaa taggatttac ttttgggata
     7681 agtgcaatga ctgctttact agcaggaact ataatagcat caacagaccc agccactctt
     7741 gtacctatat ttaatcaggt aaagataaag gatagggtaa aacaaactgt aataagtgaa
     7801 tcagctttta atgatgcaac aggagctata ctaacatcag cagtgttggc tgtaattttg
     7861 tcaaataaat tttcattagg tgaaaatatt tatgaactgg ggacaatggt tattgtcgga
     7921 gttttagttg gtttaattac aggaatactg cttttgaaat tagttaatga taaaccttat
     7981 ggaatattta aagattttgc accaataata tctgttattt cagttgtagt tgcatatgaa
     8041 ttatctacaa aattaggtgg aagtggatat atgtcttgct ttattgtagg tataataact
     8101 ggtaacaaga aaaattttaa aatttggtta agtcaaaaat cttatgatgc agatttttat
     8161 gttgctgaaa ctttaggtac tatttgtcgt atggcaatat ttataatatt gggtagccaa
     8221 gttaatttgg ctcagttaag taaatatttt atgccatctg taattacagt attagtatta
     8281 atgtttatag caagaccact ttgcgtttta gtttgtacac ttgtagacag ggaagcaaaa
     8341 tggagtaaaa gtgaaatatt atttatgatg tgggtaaggg aaacaggcgt aataccagca
     8401 gcactttgtg gtattatttc agcaatgaaa gttcctggat acgaagtgat ttcatctgta
     8461 gtattcatga ctatactaat tacattagta atacaaggaa gtactacaaa attagtagct
     8521 aaaaaacttg gattattaga agaagaagtg ataaatataa gtgaaaaagt atcaactttt
     8581 taaagttttg ctttaaaata gtacctgtta atggctattt taatattgtc taatgatgat
     8641 tattgaaata gtcatttttt tatgtataag taatttacag aatttacata ttagacatat
     8701 ttattatcaa aatttgttga agctatgata taattagttg tatttaaaat aagaatagca
     8761 aataagggag gaaatttatg tttacggtta tggatattgt acagccagat acagtagaag
     8821 aagcgtactc aattttaaat aaaagaaaaa ctaaccaagt aattggtgga agtgcttttt
     8881 taagaatggg gaaaaaaaga ataggaacag gaatagaatt atctaaatta aatttagatt
     8941 atataaaaga gtatgaagat tacgttgaga ttggttctat gactactttt agaacattag
     9001 aaacaagttc cataataaaa aataattttg gaagaataat agaagattca gttaaaaata
     9061 taataggagt tcagtttaga aatgtagtaa cagttggtgc aacagtattt tctaaatatg
     9121 gtttttctga cttgatagta gcattacttt cattagatac ggaagtagaa ttatataata
     9181 ttgggagaat tagtcttgaa gagtttctaa atagagatta tgagaaagat ttacttatta
     9241 agatatacat aaagaagaca aataagaatg cttcttataa atcaattaga aatgcaaaga
     9301 gtgattatcc aattctaaat gtatctgtat caaaacatat ggaacaattt aagttatgtg
     9361 taggagcaag accgcagaaa gctacgattg caaaacaagc tagtgaattt ctatctaaca
     9421 atgaaatcaa tgaaattaat atagataaag cagtagaaat agcatcagaa gaactaactt
     9481 ttggttctaa catgagagca tctagggagt atagaaaagc tatgagtaaa gtacttttaa
     9541 aaagagctat aatggaggtt atttaatgtt agtaaagatt aatataaatg ggaagaaaaa
     9601 aagtgttgat atagaaccag aagaatatct ggttgatact ttaagaaaag ttggtaattt
     9661 aagtgtgaaa agaggttgtg acacaggttg ttgtggttta tgtacagttt taattgacaa
     9721 gaaacctaca ttatcttgtg caacactgac attgagagct ttagataaag aaataactac
     9781 aattgaaggt ttagaaaatg aagttaaaga atttagtgaa gttttggtaa aagaaggagc
     9841 tgagcaatgt ggtttctgtt ctcctggctt tatacttact gtacttgcaa tgaaggatga
     9901 gcttgaaaat cctacagatg atgaaataaa gcattatctt acaggaaatc tatgtagatg
     9961 tacagggtat atgggacagt taagagctat caaaacttat ctggaggtaa aatagaacat
    10021 gaatatagtt ggaaaaggaa tcaagaaaat agatggaatg agtatcacaa caggaaagcc
    10081 tgtatataca gatgatttat cagcaaaaga tgctttggtg gtaaagatac ttagaagtcc
    10141 tcacgcttat gcaaaaataa aagatataga tacatcaaag gcagaaatgg ttgatggtgt
    10201 tgaatgtgtt ttaacatata aagatgtacc aaaatctcga tttacacttg ctggtcaaac
    10261 atatccagag ccatctccat atgatagatt aattcttgaa gatgttgtaa gatatgtagg
    10321 tgacgaagta gctatagtgg cagctataga tgaaaaaact gcaattaaag caatgaatct
    10381 tatcaaagta aagtatgaag tacttacacc tgttttggat tttgaagagg caataggaaa
    10441 taaaactatg gtacatgatg aagatagaca ttgtaacttt gatataggta tgcaaagaga
    10501 aaaaaatata gtatcccatc atgattatac aaaaggtgat gtagaaaaag tattaagtaa
    10561 ttgtgatgta gtgattgaag aaacttatta tactcaagct caagcacatg caatgatgga
    10621 gacgtatagg gcatacaatt atttagacca tatgggaaga ttggttgtag taagttcaac
    10681 tcaaatacca tttcatgtaa aaagacattt atctcgtgca ctagaaatac cagctagcaa
    10741 aattagggtt ataaaaccta gaattggtgg cggatttggt ggtaaacaaa ctgcatgtgt
    10801 tgagattttt tctgcaattg ttactttaaa aacagggaaa ccagctaaaa taatttatga
    10861 tagaaaagaa acttttaatt gttcaacaag tagacatgca atgagattaa aagttagact
    10921 aggtgctaca aatgatggca taataaaggc aatagatata aaagcacttt ctgatacagg
    10981 agcatatgga gagcatgctt ctacaactat tggtttggtt ggagaaaaaa ctttaccaat
    11041 gtataataaa gttgaagcaa caagatttat tgcagatgtg gtatatacta ataagatgcc
    11101 agcaggagca cttagagggt atggagcatc acaaggatgt tttgctgttg aatcaactat
    11161 aaatatgctt gctaataaat taaaaatgga cccaacagat ttaaggttaa aaaatgtggt
    11221 aacagaaggt gaaactacat ttgcatatgg taaaacttta aatagtgtcg atttaataaa
    11281 atgtataaat aaaggcaaag aacttattgg atggaatgaa aaatatcctt gtaaacatct
    11341 tgataatgga aaaattagaa gtgtgggtat ggcaattact atgcaaggtt ctggtataca
    11401 aggtcttgat aagtcttcag ctgaaataaa attgaatgac tgtggagatt atactcttat
    11461 gataggttct actgatatgg gtacaggaag tgatacaatt ttagctcaaa tggctgctga
    11521 ggttcttgat acaagcattg aaaatataac agttatatca gctgatactg acttagttcc
    11581 atatgaccca ggttcatatg cttcaagtac tacttatgta acaggtatgg cagtagttaa
    11641 ggcagctcaa gacttaagag aaaaaataat ttctgctgga gcaactaaac taggtgttga
    11701 gaaggatgaa gtagaatttg atggtataaa attaactagt aaaaaaggta gcactttatt
    11761 attcgatata gcagttgatt ctacaattgg tccagatact aatcaattaa taggtcttgc
    11821 ttctcatggt agcccagtat ctcctccacc ttttatagca ggttttgcag aaactgaaat
    11881 tgacacagaa actggaaaag ttgatttagt aaattatgta gctgtagtag attgtggaac
    11941 ggttataaat aaaaatctag ctaagataca agttgaaggt ggaattgttc aagggattgg
    12001 aatggcatta tttgaagaag ttagatacac agaaaaagga agcatggaca caaatacatt
    12061 tatgcaatac aagattccag ctagatgtga tattggaaat gttaaagtag attttgttga
    12121 aacttatgaa ccaactggac catttggtgc taaatctgtt ggagaagttg ttataaatac
    12181 acctttacca gcaatacaag aagctgtgtt taatggaatt ggtgtaagaa taaatacact
    12241 ccctattaca cctgaaaaag tttttatgga aatgaataaa taattattta tgaaaattta
    12301 gactagtttt acatgttaaa caaccagtta tgtcatacct gttgaaagat atttaataag
    12361 tagttattat aatattatga attttactta taaaatacaa aattaaataa ggggatgatg
    12421 ttttggctgg taatctaaat aatatgagag cagtaaataa ttttagagga gataagaaca
    12481 ttttagaatg tttagtcagc tttgagggtc gttcaataag tcagagaaaa gtaagggtat
    12541 tttttaaaga aaaacaaaat caaatagaaa ttgattttgc agaagaggaa atttctaaat
    12601 tggttgaaaa tgttgtttta aatacatcat atcaagaaat gttatatgat gaaatagaga
    12661 aacaactgga aattgattgt ataggtactt ggatgatatt atctaaatta aaagatggta
    12721 gtagagttca ctgattttaa aataaattat tagagcctaa ttttaaggct cttttttgct
    12781 gtttttaatt aacaatagaa aatttatctt tagtttgta
//
LOCUS       DAMJYX010000096        12686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJYX010000096 DAMJYX010000000
VERSION     DAMJYX010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.098"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(208..1785)
                     /locus_tag="PC479_002844"
     CDS             complement(208..1785)
                     /locus_tag="PC479_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2805373.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="PC479_002845"
     CDS             complement(2082..4493)
                     /locus_tag="PC479_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2805374.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4683..5084
                     /locus_tag="PC479_002846"
     CDS             4683..5084
                     /locus_tag="PC479_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2805375.1"
                     /translation="MILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFLLSNSL
                     SWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLMIDLFS
                     LNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="PC479_002847"
     CDS             complement(5149..7266)
                     /locus_tag="PC479_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2805376.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="PC479_002848"
     CDS             complement(7297..8004)
                     /locus_tag="PC479_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2805377.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="PC479_002849"
     CDS             complement(8111..9982)
                     /locus_tag="PC479_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF2805378.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="PC479_002850"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="PC479_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2805379.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
BASE COUNT         3716 a         2337 c         1364 g         5269 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcc
//
LOCUS       DAMJYX010000097        12348 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJYX010000097 DAMJYX010000000
VERSION     DAMJYX010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.099"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(40..2151)
                     /locus_tag="PC479_002851"
     CDS             complement(40..2151)
                     /locus_tag="PC479_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805380.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(2141..2893)
                     /locus_tag="PC479_002852"
     CDS             complement(2141..2893)
                     /locus_tag="PC479_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805381.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(4159..4335)
                     /locus_tag="PC479_002853"
     CDS             complement(4159..4335)
                     /locus_tag="PC479_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805382.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(4439..5047)
                     /locus_tag="PC479_002854"
     CDS             complement(4439..5047)
                     /locus_tag="PC479_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2805383.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            complement(5146..5970)
                     /locus_tag="PC479_002855"
     CDS             complement(5146..5970)
                     /locus_tag="PC479_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2805384.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(6148..7041)
                     /locus_tag="PC479_002856"
     CDS             complement(6148..7041)
                     /locus_tag="PC479_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2805385.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(7298..7735)
                     /locus_tag="PC479_002857"
     CDS             complement(7298..7735)
                     /locus_tag="PC479_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF2805386.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(7992..8141)
                     /locus_tag="PC479_002858"
     CDS             complement(7992..8141)
                     /locus_tag="PC479_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2805387.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(8154..8384)
                     /locus_tag="PC479_002859"
     CDS             complement(8154..8384)
                     /locus_tag="PC479_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2805388.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(9243..9464)
                     /locus_tag="PC479_002860"
     CDS             complement(9243..9464)
                     /locus_tag="PC479_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2805389.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            10044..10301
                     /locus_tag="PC479_002861"
     CDS             10044..10301
                     /locus_tag="PC479_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2805390.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            10770..12008
                     /locus_tag="PC479_002862"
     CDS             10770..12008
                     /locus_tag="PC479_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2805391.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         4583 a         1458 c         1672 g         4635 t
ORIGIN      
        1 aatgatttta ttactttaaa aatctaataa ccttttttat tataaaatac caataatttt
       61 tttgaatttc ttataagtta atagatatat aaggtacata taaaccccac aaatccctat
      121 aaaacctaag tttatcaaaa gagcattgac ctcactattg acgattctac catcattatt
      181 taacataatc ataaaattaa acagataaat taaaaaactt cctgcaataa gagggtatga
      241 aaatactttc tttaattgat ttctaataca gaactttata taatcattgt ttgctcctaa
      301 tcgatttaaa tccataaaga cctgcttgtg atttatagct atagtaacac cccttgtata
      361 tgcaatgatt cctactgctg taaaacatat gattgccaca tatatgaata acattaagaa
      421 gactgccatg ttttttatca tatcttggct ttgtaaaatt ttaaagttag gatagtattt
      481 ccaattaaga tacaactgat tgttgtttgg gtctagtttt aaatgacctt caccacttgg
      541 atattcttca tccataaaat attttcttcc agttgattca taacgttttt ttacgtattc
      601 atcataacca ctgaacatgc ccatttcctt agatgttctt tttataattt ctttctttaa
      661 gtcattggca aaagcatagg tatctttatc tacattaaat agtatagaat tattgtaatt
      721 ttttatggat aaatctttag taaatgattt ataatcctcc tcattaagga cataggcgtt
      781 catacttcta ttttgaaaaa aagaaccatt aaatattatt tttccagcat atctaaaggg
      841 gatttctttt tctgtgactg ggcttgatat aaatttggta tatttataat cttgattttc
      901 atttccttta tttacaacag caagatattc accatttttt atagttattg gtttgccaat
      961 tagcttggaa aaatctttgg aattaaagaa catatcatag gagctttttt tcatatatac
     1021 cttttcgata gttccatctt tatgatatat tgattctaca ccatcaacaa ttaattctat
     1081 agaaggaatt tccatatagt tttgaatttt tatattgtgt ttatctgcta attgttcaat
     1141 atcttttttt gatacctgtt tatctctttg attgtaggta aaagtataat ctatagggtt
     1201 atttttaaga gtatcataga tacctgtaac catattagga acatagaata cagcaaataa
     1261 tgctcctgca atcaaaaatg aaataacaca catgtttttt actgtttgtc ttcccataaa
     1321 tctcatcatg ctagttgata tgatgttttt atagtatttc tctggatgtt ttcctctttt
     1381 agcatgtaca actatgtatg tcataatcat atatattcca ataagagaga gtagataagt
     1441 aaagctccat atagatggta gtttataatt taatttgttt atagttagtg aaggtacgag
     1501 atatcctaga aaaataccga gtataataaa aattatacct aatataccat accagtcctt
     1561 tacatcctgt attggttcag atattctctg gtcatttaat atatcaatta tatttgtacg
     1621 attaataaat tttacagaaa gtataaatat gcataatgtc acaaatacac taaatataaa
     1681 tccatatatg aatccttgag ttccaaaatg atatgtcatt tctctggtat caataatcac
     1741 taattgaaat attttccata taccaaaaga tacaggaata cttaatataa gacctaaaaa
     1801 acagcatact attgaaatta acaatatctc tgaaaataga agttttttta atttcttctt
     1861 atctgttcct agagccatga aaataccaat ttccttgctt ttaaatttta aaaagaggga
     1921 agaagcgtat gtagtaaaga cagcgcatcc taatatagca actgagaata tcattgtagc
     1981 ctgttttctg ctatctccac cagttggaag tatgttttgt attgttgggc taaaataaat
     2041 tagagcatat gaagttacta ataccacaga aagtattata caaaagaata aagttgtata
     2101 gttcttccaa ttataggctc gaagtttact gtataattta ttaattgtca tcttgagcgc
     2161 ctcccatttc ttttaaaact tctagtaatt catctaggaa tttatgtctt gaatttgatt
     2221 ttacaacttc tttataaatt attccatctt taagcacaat aactcggtca cagaagcttg
     2281 ctgcaaagct atcgtgtgta accatgagta tagtagcatc taattcttgc tttgctttta
     2341 aaaatgattc aatgactgta cgacaagact tactatcaag gttaccagtt ggttcatctg
     2401 ctaatattaa atctgggttg ttcatgagtg ctcttgctac tgctgtacgc tgtttttctc
     2461 caccagatat ttcggcaggg tatttttttc tgatatgttc aataccaaat agctcacata
     2521 gattaattgc acggttttcc atttgtaaga cttgactacc tttgataatt tgtggaacac
     2581 atatattttc aaagacacta agaccatcaa atagcatgaa gtcttggaat acaaatccta
     2641 gttgttgatt tctaacttct gaaagttcct tttcttttaa atttcttata ttattatttc
     2701 caagaattat ttccccatta tcaattggga taaaacatga aatacaattt aaaagagtag
     2761 tttttccact tcctgatgaa cccatgattg cgacaaattc tcctttgttg atttcaaaag
     2821 aaatattttt taatacagga tatgttttac tgcctgtttt atatgattta cataattggt
     2881 ttactttaag catatatatc atcctcactt tcatttatct tgtaagtcaa tgatagcatt
     2941 tgaaagggga ggagggatat gagctatatt gtaatagttg gatgtttttt tgtaatgttt
     3001 gaaaatatat tgtttggata gtgtgagagg ttattattat agttaaattg attatattat
     3061 tggaggattt agagggttat ttttaggtat taattgttgt gctaaagtaa tatgatgtgg
     3121 aacattgaag gtgtaattag aaaagaagta tatagtaaaa gtaaaaaggt tatagaataa
     3181 cgaatgttgt attgaaaaaa atgatgtaaa taatgtatag tagggttaaa aagaaaaaaa
     3241 gaattataga gcagtaagat tatttaatgg aatgtattgt aaataatttg aaaggattta
     3301 ggggctatta acacaatgta attgagaaaa tatattggag ttttggaaat aaataggttg
     3361 ttagaaacaa ttgaaatgta taataaaaag gttaaaaagt attattagta taggtatgat
     3421 agataatttt agaaagtgga agtattttag actatgctga aaagtgattg atatgatttt
     3481 gagatattaa aatattacta tattatagta aagaagttaa aataataaaa tgttgtaata
     3541 ataaaggttt gtagtataat aaagaactgt aataaataag actttgcaat gtaatagaaa
     3601 tgctgtaata ataaaagtct gtagtataat aaagaactgt aataaataag actttgcaat
     3661 gtaatagaaa tgctgtaata ataaaggtct gtagtatagc aaagaactgt aataaataag
     3721 agattgtaat aataaaagtc tgtagtgtaa taaagaactg taataaataa gactttacaa
     3781 tgtaatagaa atgctgtaat aataaaggtc tgtagtataa taaagaactg taataaataa
     3841 gactttacaa tataatagaa atgttgcaat aacaaaacac cataataaat aagagtttgc
     3901 aatataatag aaacactgca ataaataaga gatttcaata taataaaagt ctgtaatata
     3961 acaaaatcct ataataaata agattttaca atataataaa aatcctataa taaataggac
     4021 tctgtaatat aataaaatgc tgtaacaaca aaacactata ataaataaga ccctgtaata
     4081 taataaaaat actataatat agtaaaaatc atgcaataat aaaaatctat aataataaac
     4141 ctcagcataa caaagacatt aatttcttac taaaaaggcc accacaatta acagtgaaaa
     4201 agcaattgaa agtattgaaa aagtaaagtg agaattagag tcattattac gattattaag
     4261 ttttatccat aaaccacata taattgtaca caacaataaa attgctgata ttactcctat
     4321 aaatgtaaaa aacattaaaa ttcctccccc ttatttaatg caagtatttt tagtcaatga
     4381 gaatattttc aaacgttacc ataaaataaa aaaatgtaca taaaaaatta atatagtatt
     4441 attttgtaat atctaccaat aataaatcta attgaaaatc tattaaacca tttctctgtt
     4501 caatattaag aatatcaata ccactatact tgaaaaaagc attagaacga agaaaaagca
     4561 actgaataaa tgaagttatt tgatacgcaa tagttctgta ttcagcttca gtttttttgt
     4621 tattctgatt ttcatagtga gccttaatta aaggtagtat ctcaaatgga taaacaattt
     4681 cttcctgtat caatatatag ggtacagaaa tctttataaa ttgactatgt tcacctataa
     4741 aatcaaaaaa tgcaattagt atttccctta attgctgttt aggtgctaaa tttttagtat
     4801 catgtgtagc cttatattca tccatatgac gctttaaaaa ttttttaata gaaatattaa
     4861 ttaactcctc tttagattta aaataataat taatcatagc taaattgaca tttgctttag
     4921 ctacaatttc tctagccgta atgcttgaaa catctctatg tgaagatagt aactggattg
     4981 tggcattaat taattgttcc tttgttgaat ctgctttcat aatttttccc cttaaaagtt
     5041 tctccataaa aaaattaaac atgtttaata cacgtttaga tactatcatg aaaattttgg
     5101 gatgtcaatg ggtgattagt acttaagtat ttaagtacta atctattaga cttttataag
     5161 ggggatttga atttctgtaa gataattttc aggatttatt tcattatgtt cacctttatg
     5221 tccgatttgt ctatttatac ctgatatttg ataatcacta tttttatcta accaataaca
     5281 gaacgattca tatgcaccag ctatattttc atatggacca taaaccatag tacatgccat
     5341 catatcaatt tcttctgtct cacgatatat aaatccagat ttgttctttc caatcttctt
     5401 tacaaccatt ccaacttcaa tatctacacc attatctttg acttcttcat catggtaaaa
     5461 agctatgtta ttgttggatt gacgtgatac ttctatatgt tcttctttga taaatttaga
     5521 tagcttttcc cagagtatac cttcagattg gtagtctgga attatctctc taagtgatat
     5581 gattttataa cttggaatct ttttaaagac aacattgtaa tgaatttcat ctttatcaca
     5641 gtttatagct tctataaatt tatcaatttt atttattctt tgttgttcct gctttatttc
     5701 cttttgtatt tcattctttt ttctattcaa ttccttaatt acaaattcat cattccaatt
     5761 atgtacgata tttgctattt ctgcaacgct aaattttgaa tctctcaata atacaattct
     5821 ttgtaatatt gaaatctgtt caactgagta taatctatga cctgtatatt tgtctgtttt
     5881 tgcaggtttt aaaataccaa gttcatcata ataacgcaac atacgaatag aaatttgtgt
     5941 tagttttgaa acctctccta ttttaaacat gaaaatatga cctccttttc acttataatc
     6001 agtatatatt ttgtttttat atacttcaag cgtttgtgga aatacaatta ttttccagat
     6061 gttgcaataa attatagaga gtaaattata tttaagtagt aatcgtatca aatcatagat
     6121 tatcttaatt tataattcat attaaaatta ccagtctaac tcaaattcaa aatcatccga
     6181 ctttatctgt tcaatttcag aatttacctt ctgtttaaat ttattagaag aaatcatata
     6241 tacaagaaat attaaaccaa atattatttg tacatattga gctattacac ataatttaat
     6301 agggtagtgt agaagtaatt gaatgttgaa aaaatagtac atcatatgaa ctaatccagc
     6361 tgataaaatg cttctacttg tattgatata tacaaaatta attataaagt ttaaaagtac
     6421 aatgtttggt ataaataaaa aagcatcaaa tagggagtat tgtgccaaat tatatggtat
     6481 ttgattaggt gtaaaatacg accctagata ccagattcca caaacaagcc ctaaaataat
     6541 tgaagagcct ataaatccaa atctaactaa taatttgtca atggcatatc ctctccatcc
     6601 agcttcttga ctaagtatac tcaatataaa acttgctaat agcatataag ctattttaga
     6661 aggtctaaac actgctatgt gtatccaatc tatatcagaa gtttttacat taaagtaatt
     6721 tccgattatt aacgaagtaa taaaagttaa agcaaaaaat aatattgtta atagtatcca
     6781 ttttattccg atttctttaa agcttaggta tctataaata aaatcttgtt ttgcactttg
     6841 tggatagatt gtagaaataa aaaatagacc tacaatagct ggtgatgtat atgcaagata
     6901 aaaaatgatt tctccaagta ttgtatttgt aattccgagc aacgcaggtg taagactgaa
     6961 tatccagttc aatactagag taaatgtaaa gaaatagatt aagtgtttgt ttgtattaaa
     7021 gggggtgtta tatagttgca ttttttcact atccttccgt tttataatga agttttaatt
     7081 tttattaatt gtttatttat ttttatatgt attaagttat cgcttgatgt tagttattgc
     7141 ctataataat atcctatttt ttatattttg attaattatt tttacactaa atataattaa
     7201 ttagtattta atattgaata tattatatta ttattaatta ttaaataggt caataaattt
     7261 gcaattagtg atattaataa attactttag atttttatta aatgtttagc ttttctttaa
     7321 tattttttat ataacgacga tttataataa ctttttcgtt gttttttaaa gtagcttgca
     7381 tgcgattatg tgatattact tttatacttt ttatgtagtc catatttaaa atcatggatt
     7441 tagatattcg tgagaaagat gtattagcta ataattttga taatttatat atttttaaat
     7501 caatttcaaa tacatcatct tcataataag caaatgtttt atttccaact gtctcaaagt
     7561 ataaaatatc acagacattg agtttataaa tctctcctga ttttttacct tttatatcaa
     7621 gtaacggtat actcaataat gaaattattt tttcaatgtt attatctaga gcagaacatt
     7681 ttatggttat ttcaatatca tcacaattaa taccttgttc gacatgtatt ttcattaatc
     7741 atcacctcat tttattactg tcattagaat agcattatat cttattgagt ccaactagat
     7801 gcgttctaaa tttcaaatta agcatagtga aattcaaaaa taaaacagtg aaattcaaat
     7861 ttgaggtggt aaaattcaaa aaaatactat ggatatttgg ataaattatc caaatatcct
     7921 agtatttcac atagaatatg tctaattatt ttacaaatag acctatttta tgacttacat
     7981 atattgtcaa attacttctt aaatatcaaa atatttttgg ataatgtatc aatactgttt
     8041 gataaagtat ttaatttacc ctcaattcta accaacaagt acatggatag agctatagga
     8101 aaaccaacat tggttattaa cagttgtaaa ctatcatcca taaaattagc cccctattct
     8161 ataactaagt cataaggagt ggtatttgtt tccacaacct tggcttcaac aacagaagct
     8221 aggtcaaatc ctccaaccga aaaaatattt ttagatacta tcaaatccat gacagatttt
     8281 atattttctt cagttaaatc atcctttgga ttttctatag aaaaagaaac ttgtttatcc
     8341 tttacatttt taaagctcat gactaatttt ttcttaattt ccatataatt acctccgata
     8401 taataattta tattttaatt gtttgcttgt taatattggt attttaatta tcaatattaa
     8461 tgttttgctt gttagtatta atatttcaat tgtcaatatt aatgttttgc ttgttagtat
     8521 taatatttca attgtcaata ttagtgtctt gcttgttagt atcagtgttt caattgtcaa
     8581 tattagtgtt ttgcttgtta gtattaatat ttcaattgtc aatattagtg ttttgcttgt
     8641 tagtattaat atttcaattg tcaatattag tgtcttgctt gttagtatta atatttcaat
     8701 tgtcaatatt agtgttttgc ttgttagtat tagtatttca attgtcaata ttagtgtttt
     8761 gtttgttagt atcagtgttt caattgtcaa tattagtgtt ttgcttgtta gtattagtat
     8821 ttcaattgtc aatattagtg ttttgcttgt tagtattagt atttcaattg tcaatattaa
     8881 tgttttgctt gttagtatta gtatttcaat tgtcaatatt agtgttttgc ttgttagtat
     8941 cagtgtttca attgtcaata ttagtatttt aattgttgac tctagttatt gctgatatag
     9001 aaaatattta atttatttca attaattaga ttaaagtagg gtagcatcta aaatagctaa
     9061 catgtttaag ttgaaatacg tttaatatat taatttttca aagcgttaat atattaaagt
     9121 tataaccaaa attgaagtta taatctgcca aatctatagc gtattaagtg cattatataa
     9181 cttaatcttt tatataaaga gttaattttt taatttgtaa agcagagtac gtttaaaaga
     9241 cactatttag atagtgatgt actatctaat tttaaaatat ctacaatagg gttctcttga
     9301 agacttgcta ttgcttctgc aacagcataa acatcttcat ttaatgcctc tgcttttaca
     9361 tttgcaaatg atttactttt aacaactgcc ttacccttat catcaaaacc agcatccaat
     9421 tttagtttta tactagataa attttttgtt gaaacaactt ccataaaatt acctcctaaa
     9481 aatttaattt caaagatttg tcgactttct ttgttagcta taatactatc atctaagata
     9541 atatcggagt taaacagtaa tttctaaaga ttttttgtgt aaatttttat aggaaaaatt
     9601 ttactttttt gctgatgaaa aagtttacaa gttatttgtg aagatatgtt cttagtgttt
     9661 tgaaatagta tttgtatata ttcatgctta gttgagatag ttttgtatgg attttcatat
     9721 gtgtagagtt aaatgttagg tactaaagtt tatgttaaat taaatgttaa agcttatgtt
     9781 aaaaatttta ttaaatttaa tctgtaaata aaaaagttaa aagtccaata ttaacatttt
     9841 acttttaaac cattaaaatc tttattaaag ctaacaaagt tataagttat gagaaatttt
     9901 attgaagtta atacaaaaat taattttaaa gctaagaaat tttttattag attcaataaa
     9961 actaataaat atagtttagt caataatgta ttaaataaaa actttgttaa gtctataaaa
    10021 atatcttaaa ggaggtgtaa cttatgaaga taaaagtgga ggatatacca gttcaatttc
    10081 atgcaatggt agaaataatc ggaattgata agttcataga ggttgccaag ttgtatggag
    10141 gaacaaatac ttatatacct acgtataagg ggctttttag atatgctaga aatagggata
    10201 tagtaaaaca atttaatgga gtcaaccaca atgaattggc tattagatat aatatgtgtg
    10261 taagcaatat taagcgaata ctcaatgaaa atagtgttta aatataataa aataatgttt
    10321 aaacataatg aaaatagcct tgttaaacgt aagagtaaag acaaagaaga tgttttataa
    10381 agttttttta gacaaattaa ctaaatttac tacttattaa tttaaataca ttgtatattc
    10441 tataaaaaaa tttgttaaat actggagata tattgagaat acaaagaaaa tggtcgaaaa
    10501 tatttgttgg ctaaaaaata gataaaatga tacaattgct tgtataaagg ggaaaaataa
    10561 ataaataaac tatataaata aagaaatagc aattaatatg acataatata tttataaaat
    10621 taagaatata aatagatatt ttaagggatt agttgttaaa ttaggaaata ttaaaaaaaa
    10681 tatatattta tatttaaact attttgttat ttatataaaa atacttatct agttactagc
    10741 aattattagt agaaatgggg gaaatagaga tgaataaggg attttacaaa ttaaattcag
    10801 tggtattgaa ctttgatatt aagaatgatg ttaacaagat aagatttaat cattatataa
    10861 tgagaaaaga aaacatacaa agacttcaag gaactctgcc aaacggacaa ttttatatga
    10921 cagtgagaag agctgctaca gatttacaac tttctatttc tactataagt aggcttgtaa
    10981 aagagttttt ggatttaggg ataataagat tagtgtctaa gggtgtaaaa ggtaatcgtt
    11041 ctgtatatag ttacgtaagt atagagttta atggaagttt atttgatgat agtaaatata
    11101 tggaacttga ctgtgtatgt aatgatgaat ttattgataa tagtggcata gagtttgatt
    11161 gtgatagcaa agataattct tttaatggag atataaatgt agaaactgtg cattgcatta
    11221 atgagtattt ctctgatgat gatgtagagc ataattataa caatttccat aacaaagata
    11281 ctataaagga taaagccatt tctaatatta gcactatcaa aaaatatatt gatgaatctt
    11341 ttgatacaaa taaatcagaa aattctaact tacaacagga gtcaataaat ttcaacttta
    11401 atgatacatg caatgaaata gataaaagaa cgaataatag tatagaaaat aatcaaaata
    11461 aaaggcctat gagattgagt gacatcagtc ttttaagtga taaaggtatg acaaataaaa
    11521 agaattactt taatcataat catggtatac aaaaagggac taaatctgta acaaagaaaa
    11581 aagaatcctt aaataaattt aataaaaaaa atatacagca aagcattata aatatgctaa
    11641 ataaaaaatc aggtaagaat tttaaaatcg attctccaat aagcataaaa cttatcaatg
    11701 aaaggctaaa ggaaggctat gtcttagaag atttctacaa agttattgag gtaaaagtgg
    11761 ctaagtggaa aaataccata atggaaatgt atataagacc agaaactttg tttagctata
    11821 agtttgaatc ctatgtaaat gaaaacatat ctatgaaaag tcaaagtaat gttcacccat
    11881 gtaaagaagg caatagtaat gatagcagta attatataca ttctgattat ataacatatg
    11941 atggcagtgt gcgtgagaga aaaaaatatg agaatgtaag atactggaat cctatatgtg
    12001 aaatttaatt agtttaaaaa tgtatttatg ataaataaga tattaataga gaatagtgag
    12061 ctatagcaaa tgatatatca atagttggta gtaagttata ttaagtaaga tattactatc
    12121 aggtagtgaa ttatattgaa cgatatatta ataattggca gtaagttata tcaaataaga
    12181 tattactata gagtaatgaa ttatattgaa cgatatatta ataattggca gtaaattata
    12241 ttaaataaga tattactata gagtaatgaa ttatattgga cgatatatca atagttggta
    12301 gtaagttata ttaagcaaga tattactatc aggtagtgaa ttatattg
//
LOCUS       DAMJYX010000098        12324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJYX010000098 DAMJYX010000000
VERSION     DAMJYX010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.100"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            291..635
                     /locus_tag="PC479_002863"
     CDS             291..635
                     /locus_tag="PC479_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2805392.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            666..2267
                     /locus_tag="PC479_002864"
     CDS             666..2267
                     /locus_tag="PC479_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2805393.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2286..3386
                     /gene="dprA"
                     /locus_tag="PC479_002865"
     CDS             2286..3386
                     /gene="dprA"
                     /locus_tag="PC479_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2805394.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3419..5506
                     /gene="topA"
                     /locus_tag="PC479_002866"
     CDS             3419..5506
                     /gene="topA"
                     /locus_tag="PC479_002866"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2805395.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5671..6456
                     /gene="codY"
                     /locus_tag="PC479_002867"
     CDS             5671..6456
                     /gene="codY"
                     /locus_tag="PC479_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2805396.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6563..7828
                     /locus_tag="PC479_002868"
     CDS             6563..7828
                     /locus_tag="PC479_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2805397.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7859..8629
                     /locus_tag="PC479_002869"
     CDS             7859..8629
                     /locus_tag="PC479_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805398.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8738..9184
                     /locus_tag="PC479_002870"
     CDS             8738..9184
                     /locus_tag="PC479_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2805399.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9202..10395
                     /gene="nifS"
                     /locus_tag="PC479_002871"
     CDS             9202..10395
                     /gene="nifS"
                     /locus_tag="PC479_002871"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2805400.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10449..10889
                     /gene="nifU"
                     /locus_tag="PC479_002872"
     CDS             10449..10889
                     /gene="nifU"
                     /locus_tag="PC479_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2805401.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11178..12257
                     /gene="mnmA"
                     /locus_tag="PC479_002873"
     CDS             11178..12257
                     /gene="mnmA"
                     /locus_tag="PC479_002873"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2805402.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
BASE COUNT         5168 a         1264 c         2268 g         3624 t
ORIGIN      
        1 atagttatga ccggatacaa tgtattttca acatttttca aatagcttac aactaatata
       61 aataaagata aatatcgtat aaaagcaact aatttttctc tgttcattaa aggcactcct
      121 ccaaaaaaat aaacttttcc tatgaaaact agaataatat cataaaagaa taaagtcaat
      181 agaagtattt attaatattg atggaaataa aatcaatcca aaaacttgaa tagtaaaatt
      241 gtatactgac cataatttaa aagtgaaaat ttaatgtatt ggagtgaata atgaataata
      301 aagaaaaagg tgattttgga gaaaaagtag cagttaatta tttgctatca aaaggagcaa
      361 agatactaga aaaaaattac agattaaaga taggggaaat agatataata gcaaaaatgg
      421 aagatgaaat tatatttgtg gaggtgaaat caaggtctaa cataaagttt ggctaccctt
      481 gtgaatctgt aagcttcaaa aaaagaaaaa agataattgg agttgcaaat tattatatta
      541 taaaaaataa cttaaataac actcccataa gatttgatgt tatagaggta tatttattag
      601 aaaaaagaat aaatcacata atgaatgctt tttaaaaaag ataatgtttt tggaaaggat
      661 taaatatgtt aagtattatt aattcaagca acttagtagg aatcgacagc tttttagtta
      721 aggttgaagt agatgtaagc aatggaatac ctagttttaa tattgttggt cttccaggca
      781 aagaaattaa agaagcaaga gaaagagtaa agtcagcaat tcttaatagt ggatataaat
      841 ttccaagcac gagaatagtt gtaaatttat caccagcaga tataaaaaaa gaaggagctt
      901 ttttagattt atctataagt ataggccttt taagagagtt aataaaaaaa gatgaaaatt
      961 acataagaga aagtatgttt ataggagaat tatcattaga tgggaaaata agaaaagtaa
     1021 gaggtatatt gcccataatt atgggtgcaa aaactcagaa cataaaacgt atttttatac
     1081 caattgagaa cataaaagaa agtctatttg tagatgaaat tgatataatt cctattaaaa
     1141 gtttaaaaga atgtgtcgat tttttaaacg aagagataaa agtcgataaa gtaaggataa
     1201 tgtctttttt ggatgataag tcaagaaaag agaacgaaga attagaaaaa gataatagtt
     1261 atatagattg caaatatact aagattaata atgaagaaag taaatatgat gaagacttta
     1321 aagatgtaaa aggaaattat tttgtaaaga ggagtgcaga aattgctgct gctggaaatc
     1381 ataatatgtt tatgattgga cctccaggct caggaaaaac tatgattgca aaaagagtaa
     1441 ggacaatttt accagatata agtgtagaag aaatgataga agtaagtaag gtctatagta
     1501 tattgggtat gataaatgaa actaaaggaa taattgataa aagacctttt agagcacctc
     1561 atcatacaac tacaaagcaa tctttaattg gaggaggaat ggatgcaaga cctggagaaa
     1621 ttgctttagc acatagaggt atcttatttt tagatgagat agccgaattt gatagaaaaa
     1681 tacttgagac tttaagacaa cctatagaag atggatatgt aaatatatcc agagtaaagt
     1741 acagtgcaaa atatccttgt agagtactgt tagttgcagc tatgaatcca tgtccatgtg
     1801 gttattatat gtcagagaca gagtgtagat gtagaagtaa cgaaattgat agatacataa
     1861 ataaaatatc aggtccattg ttagataggt ttgatatttt tgtagaagtt aattctataa
     1921 aatattcaga ttttaattct ttaaaacaag aagaaagctc tcaaaaaata aagagaagag
     1981 tagaaaatgc tagaaaaata cagataaata gatttaagaa agataatata aaaaataata
     2041 gtgaaattaa agcttacaat ttatttaaat attgtaaact ggaaaaagaa gcttcaaaaa
     2101 cggctgagat gatatttagt aaatacaacc taagcagtag aagttataca aaacttttaa
     2161 aaatggcaag aacaatagca gatttagaag aaagagattt gataaattca cagtgtatta
     2221 tagaagcttt ttcatttaga aaggcttatt atagttattt taaatagttt tttgaggtgg
     2281 attttatgga aaaaagagat acttatttat ggttaaaatc aattggtgga ataacaacaa
     2341 aaacaattga aataatagaa aatgaaatag taaatattga agatatattt gatttttctg
     2401 aaaaagaaat atataattta aaaaatataa gcttgaatat tagaaaaaat atagtaaaat
     2461 atagaggtca cgcttattta gaaaatataa aagaactact ttacaaaaaa gcgattaaat
     2521 atatttgtaa atatgataaa gaatatcctg aaaatttaaa gaatatatac aacgctccaa
     2581 agttattatt ctacaaaggt gacataggct tagttaataa taattttaat atagctatag
     2641 taggttcaag aaaacctaca gcctatggaa ttaattgtgc aaaaacaata agttgtcaac
     2701 tatcccaata tggagttaat atagtaagtg gattagctat aggaatagat gcatactcgc
     2761 acataggttg tatgagtgga aaatctaaaa ctatagcagt acttggttca ggagtagaca
     2821 atcctcttcc aaaacaaaat ttacatttat caaataaaat attagaaaat gggggattat
     2881 tattatcaga gtataatatt aactcgacag tagccccgta ccatttttct aatagaaata
     2941 gaattatcag tggtttaagt gatggagtag ttgttgtaga agcagcaata aaaagtggtg
     3001 cactaatcac agtagatttt gctcttgaac atggaaaaaa tgtttttgca atacctggta
     3061 atataaattc tcaaatgagt agaggttgtc ataaaattat aaaagaaggt gcaaaattaa
     3121 ttgaaaatat agatgatatt ttaaatgaat ataacatatt taatattatt gataaaaaaa
     3181 taaatcaaaa atatgataat ataagtctaa atgccaagtc taagcaaata attgaagcta
     3241 taaaaagaga aggaaacttg catatagata gtatttgtga ttatactggt atagaaataa
     3301 agtatgtaaa ttcaataatc aatgaattag tactaaatga gttagtagta gaaatgaata
     3361 ataaaacgta cagtttaaat gtataaatta agattaggta gtgggggtgt atggttttat
     3421 ggccaaaaca ttagtcatcg tagaatcgcc tgcaaaagct aaaactatag aaaaattttt
     3481 agggaaaagt cattacacag ttaaagcatc agttggtcat gtcagagatt tacctaaaag
     3541 taaattaggt gtagatatag aaaataattt tgaacctcaa tatataaata taagaggaaa
     3601 aggcgatgta ataaaagaat taaaaaaaga agctaaaaaa tctaaacaag tatatctagc
     3661 aactgaccct gatagagaag gtgaagctat atcatggcat ttagctcata tactaaattt
     3721 agatgaatct gaggattgta gaatagagtt taatgagata actaaggatg caattaaaaa
     3781 agcaattaaa catcctagaa atattgatat aaatttggtt gatgcacaac aggcaagaag
     3841 agtgctagac agacttttag gttatcaaat aagtcctata ttatggcaaa aggttagaaa
     3901 aggtcttagt gcaggtagag tgcaatctgt tacaacaaag ttaatttgtg atagagaaaa
     3961 agaaataaag gcatttattc caaaagaata ctggacaata gatgttgagg ctaagacagt
     4021 aaaagacaaa gatataactt tgaaatttta tggtaaaaat gatgaaaaga ttgaattaga
     4081 aaatgaagaa gctgtaaatg aaatactaaa agaaatggaa ggtaaagatt taaaggttga
     4141 gaagatagaa tctaaatcaa gacgtaaatc agctccaaaa ccatttacaa caagtatgct
     4201 tcaacaagaa ggagcaaata agttattttt tactactaaa aaaacaatga tgatagcaca
     4261 agaattatat gaaggtatcg acatagaagg tgaaggtact gttggtctta tatcatatat
     4321 aagaactgac tctaaaagaa tatctgatga agcaaaagaa aaagctaagg gttatatcgt
     4381 aaacaattta ggagaaaact actataaaaa cattggtgat aaaaaagaaa aaaaggatac
     4441 taaaaaggtt caagatgccc atgaggcaat aagaccaacc tcagttgata gaactcctga
     4501 gagtataaaa gcctcattga gcaaagacca gtacaagtta tacaatttaa tttggagaag
     4561 atttgtagct agtcaaatgg aagattcagt gtttgatata ctaaatgtag aatgtaaaat
     4621 aggcgatatt gtatttaagg caactggttc aaaaatgaaa tttgatggtt ataccaaggt
     4681 atataatttt tcagaaagag aagacaaaat tttaccaagt atagaaaaag gagatatatt
     4741 aaatatagaa aatatattgc cagaccaaca ttttacacaa ccaccagcaa gatatacaga
     4801 agctagttta gtaaaaacac tagaagaact aggtataggt agaccaagta cgtatgctcc
     4861 aacaatagct actatactga atagagagta tgttgaaaag caaggaacaa gtctatgtcc
     4921 aacagaactt ggaataatag ttactgatat tttggaaaat aatttccata aatttataga
     4981 tgtagatttt actgctgata tggaaagtaa acttgatgtt atagaagaag gcaatacaga
     5041 ttggaaaaag attgtatcag aatcatatgc accacttaaa gaggctattg aagaagctct
     5101 tgaaaatata gaaaaagtaa atatggatga agaaactgat gagatttgcg agaactgtgg
     5161 ttctaatatg gttatcaaat atggtagatt tggaaagttt atggcttgta aaaactatcc
     5221 cgattgtaaa acaactagac caattatgaa taaaattggt gttaaatgtc caaaatgtga
     5281 agaaggagat attatactta gaaaatctaa aagaggtaaa gctttttatg ggtgttctaa
     5341 ttatccaaac tgtgactttg tagcttggaa taagccaaca ggagagtttt gtgagaagtg
     5401 tggttcatat atggttgaaa aagtgactaa atctgaaaca aaaacagtgt gttctaataa
     5461 agaatgtaat aaagagacaa aagagcataa tgacggaaaa gagtgaaaat tgaaaaaaat
     5521 cacataattc tcttgaaaaa ggttccaatc tatgataatc tttcaagtgt aaacaactaa
     5581 tagaaaaata aattttcatt aaatgtgaaa ataatattag aaaatataaa aggttattaa
     5641 aaggcctaaa tttcatgagg agatgattaa atggcaagtg aagtgttaca aaaaacaagg
     5701 aaaataaata aaacattaca aacaagtggt ggaagcagtg tctcttttga tttactggcc
     5761 ggagcattgg gcgacgtttt aagttctaat gtttatgtag taagtgcaaa aggtaaagta
     5821 ctaggtcttc atttaaatga tgttcaagac agttcagtta tagaagatga gtatactaag
     5881 caaaagaaat tttcagatga atatactcaa aatgtgttaa aaattgatga aacattagaa
     5941 aatttaaatg gtgagaagat attagaaatc tttcctgaag aacatggaag attacaaaaa
     6001 tatactacag tagttccaat attaggaagc ggtcaaagat taggaacatt ggtactttca
     6061 agatattcaa attcattcaa tgatgatgat ttagtaatag ctgaatacag tgcaactgtt
     6121 gttggtcttg aaatattaag agcaataggt gaggaattgg aagaagaaat gagaaagaaa
     6181 gctgtagttc aaatggcaat aggcactctg tcctactccg agcttgaagc agttgaacat
     6241 atttttgctg aattggatgg aaaagaaggt ttacttgtag caagtaagat agctgataga
     6301 gttggtataa ctaggtctgt aatagtaaat gcacttagaa aatttgagag tgcaggtgtg
     6361 atagaatcaa gatcattagg tatgaaaggt actcatataa gaatacttaa tgacaaactt
     6421 acagatgaat taaaaaaatt aaaaaacaat caataatcta tattttatag gtttagatta
     6481 gaaaaggtat ctatttcaaa tagatacctt ttttagacga attattaagg taatgataag
     6541 tatattaaaa aggagtgaga aaatgccttt agcagataaa ataagaccaa aaacaatgaa
     6601 tgatgtaata ggtcaaagtc atataatagg taatggaaaa atattgtcta agatattaca
     6661 atctaatttt ttaccaaata tgatattttt tggtcctcct ggtgttggta aaactacagt
     6721 agcggaaata atagcagaaa gaagcaataa aagcttctat aaaataaatg ctactaattc
     6781 atctttagaa gatataaaaa aggtgatatc agagcttgga agtataaata atgtaaatgg
     6841 agtcctatta tatatagatg aaatacaaag ttttaataaa aaacagcaac aatcaatatt
     6901 agaatttatg gaaaatggga gtataacttt gattgctagc actacagaaa atccatatca
     6961 ttatgtatat aaagctcttt taagtaggtc tactgtattt gaatttaaac ctttagaaaa
     7021 acttgatata gaaaaaggac tgaaaagagc tgttgaagta ttaaatgaag atagctatat
     7081 ggatattgag tgtaataatg atgctattga agatatagca attctttctg atggagatat
     7141 gagaagagcc ttaaatattt tggaagtggt agtctattca actaaaccaa ataaggataa
     7201 tataacttat atagattcag atgtaataag ggcatctaca tttaataaaa ttataaacta
     7261 tgacaaaaat ggtgatagtc attatgatat tttaagtgca tttcaaaaat ctataagagg
     7321 aagtgaccca caggcatcta tacactatct agcaagactt ataaaaggtg gggatttgat
     7381 aagtatatgt aggagacttt tagtaatagc tagtgaagat ataggtttag cttatccaaa
     7441 tgctattgta attgtaaagg catgtgtaga ctcagctatg caacttggat ttccagaagc
     7501 gaaaatacca ttagcagaag ctactattct tttggctaca tctccaaagt ctaattctgc
     7561 atgtatagca ataatgaaag ctatggatga actggataaa gagtttgtta aggatatacc
     7621 aaatagtata aaggatgctc actattctgg ttctaaagaa ataggtaggg gttgtggata
     7681 caaatatcct cataatttta aaaatcatta tgtaaagcaa cagtatttac cagatagcat
     7741 aaaagatagt atttattaca taccacaaga aaataaaact gagaaaaata taaaaaaata
     7801 tttagaatat ctaaatagtg atttttagac tgaactttat gtatcggggg tagatattat
     7861 gattataaaa cttaactcaa cttatcacag tagagttatg aaatacttaa aaaaagaacc
     7921 tgagtacaat ttgtttataa taggggatat tgaaagatat ggatatggaa ataatttttt
     7981 aaatatatgg gcagatgtgg gtgaacatgg tgaaataaag gcaatattac ttaaatattt
     8041 tgaatttatg atgttttatt ctgatggaga gtatgatgtt gaaggttttt ataatctact
     8101 tcgtaatact aattacgaag aaatatctgg gaaaatatgt gctgtagatg ctttagcaaa
     8161 gagattagga cttaataatt taaaggtagt tgatttttgc aaacttcaaa ctaaaaagtt
     8221 tttgatagat aataattgta atgcaaaggt taagagaatt aggcttggaa atctaaaaaa
     8281 gacagtaaaa ctttatgact taatagatga atttcacagt acaactttag agaatttaaa
     8341 aaatggacta aggacaggta gaggatattg tatagagatt aacaaacagg tggtgtcaat
     8401 ggcaaagagt acttcagaaa ataggactca tgcaatgata attggagtag gaacacatcc
     8461 taaatataga gctaaaggac ttgctacaaa atgcctaatt aaactatgtt cagagttact
     8521 gagagaaaac aaaattcctt gtttgtttta tgataatgaa gaggctggaa agatatataa
     8581 aaaactagga tttgaaaaca ttggaaaatg gggaatttat tcaaaataaa aaattaaaat
     8641 taatcttgac aaaattagta gggtataata aaattatctt gaaagtaatt cctactaaaa
     8701 cagtatgaat tagatttcta tatagaggtg ataatatatg aagttgtcta ctaaaggtag
     8761 atatggacta aaagcaatgt ttgaattagc attaaatcaa gacaatggac cagtatcatt
     8821 aaaatttatt gctaagaagc aaaaaatatc agatcagtac ttagaacaaa tattttcatc
     8881 tttaaaaaaa tcgggtctag taaaaagtgt gagaggtgct caaggaggtt atttgctttc
     8941 aaagaatgct gaagacataa cagtaggaga tatacttgta gtattagaag gaccagttgc
     9001 attatctgac tgtgtactag atgaagatgt atgtgaaaac tcaaacatgt gtgtaacaaa
     9061 aatagtttgg gaaaagatga aaaagggtat agaagatgtt atagattcaa ttactcttaa
     9121 agatatgata aatgactata acaaaaataa attagaaaat gatataacaa atattaaaaa
     9181 atagatttgg agatgaatgt aatggaaaaa agaagattgt atatggatta ttctgcaaca
     9241 acacctatta aaaaagaagt attggatgca atgatgccat acctaacaga ttattttggt
     9301 aatgcatcta gttttcacac attcggaaga gaagcaaaag atgctttgga taaagcaaga
     9361 gaacaagtag cagcccttat aaatgctgaa cctagtgaaa tatattttac agcaggtggt
     9421 tcagaaagtg ataactggac actagagggt gtagcttatg ctaataaaaa caaaggtaat
     9481 catataataa cttcaaaaat agagcaccat gcaatacttc atacatgtga atacttagca
     9541 aaacatcatg gatttgaaat aacttattta gatgttgata gcgaaggaaa agtagactta
     9601 aagcaactag aagattcaat aaaagatact actatactta taagtataat gtttgcaaat
     9661 aatgaaatag gaactataca gcctattaaa gaaatatccg aaatagctaa aaaacataag
     9721 atattattcc acactgatgc agtacaagct acaggtaata ttccagtaga tgttaaagaa
     9781 ttaggaatag atttgatgag tatgtcttca cataaaatat atggaccaaa aggtgtagga
     9841 gctttataca taagaaaagg tgtaagactt cataactttg tccatggtgg agcacaagaa
     9901 aaaagcaaaa gagctggtac agaaaatata cctgcaatag ttggatatgg aaaagcagca
     9961 gaattagcta aggaaaatat gcaaaatcat gtagaaactt taactcgtct tagaaataag
    10021 ttaatagatg gtgtgttaga aagaatacct tacacaagag taaatggtag cttagaaaat
    10081 agattgccag gaaatgcaaa ctttgcattc caatttatag aaggtgaagg aatactttta
    10141 ttattagata tgttaggaat tgctggttca agtggttctg catgtacttc tggttcatta
    10201 gacccttcac atgtacttct tgcaataggt ttaccacatg aaatagcaca tggttcatta
    10261 agacttacag ttggagattt tacaacagat gatgatatag attatatact agagaactta
    10321 ccaaaagtaa tagaaagact tagaagcatg tcaccacttt atgatgatgc gaagaaacaa
    10381 ggtttagtaa aatagacata aagataaagc ataaaaaata gacataaatt gagtaggagg
    10441 aaaagataat gcaatatagt gataaagtta tggaacattt tatgaatcca agaaatatgg
    10501 gagaaattga taatgcaagt ggtgtaggtg aagttggaaa ccctacatgt ggagatataa
    10561 tgaagatatt tttagacata gatggagatg taataaagga tgttaagttt aaaacatttg
    10621 gctgtggttc agctatagca agttcttcta tggcaactga aatgataaaa ggaaaaacta
    10681 taaaagatgc attagaactt acaaataaag ctgttgcaga agctttggat ggtttaccac
    10741 cagtaaagat gcattgttca gttttagctg aacaagctgt aaaagcagct cttatagatt
    10801 atgctcaaaa gaacaatatc catataccag aattggatgg atatgttata gatgatgctc
    10861 atgaccatga tgttgaagaa gaagaataaa aataagtgat tgtgttatga gaagtttaca
    10921 atgattaaaa agaagtacta atgatataat taggacataa aaaatagctg tttttaaaat
    10981 agctattttt tattgcctaa attaagtatt ttaacttgat ttagaggtta attagttaac
    11041 aaaataatcc ataagattca aataataaag atgattattt ttttagaaaa tagaacaagc
    11101 tatattttaa ataagtacaa tacttgctct atatggtata ataataagta atgcaaaaga
    11161 tgaggtgaaa tttgtttatg aataaaaaag taatgatagg tatgagtgga ggagttgata
    11221 gctctgtagc tgcatatctt ttaaaacaac aaggatatga tgtcattggt gttactatga
    11281 agttgtggca agatgatgat gttgttgaaa ttgaaggtgg ttgttgctct ttatctgctg
    11341 ttgaagatgc tagaagggta gcaaataaaa taggtatacc attttatgtt ttaaatttta
    11401 gagaagtgtt taaagaaaaa gtaatagatt attttataga tgaatatttg gaaggaaaaa
    11461 ctcctaatcc ctgtatagct tgtaataaac atataaaatt tgatgatttt tataagaagg
    11521 ctagacaaat aggttgtgat tatgtagcta caggtcacta tgcaaagata gaaaaggatg
    11581 aaagtacagg aagatatttg ctaaaaaaat ctgttacaga taaaaaagac caaacttatg
    11641 cactttataa tcttacacaa gagcaactag agcatacttt attgccaata ggtgactatg
    11701 aaaaagatag agtaagagaa attgctaagg aaatgggtat ggcagtacac aataagcctg
    11761 atagtcaaga aatttgtttt gttaaagata atgactatgc aaattatgta aaaaaacatt
    11821 ctaaaaagcg tattgaagaa ggtttttttg tagatactaa aggcaatata cttggaaaac
    11881 ataagggtat attatattat actataggcc aaagaaaagg gcttggaatt acttttggaa
    11941 aaccaatgtt tgtaatagac ataaatccaa taaataatac catagttctt ggagacaatg
    12001 aagatttatt taaaaaggaa cttattgcta aagatgttaa ctttatatca atagatacgt
    12061 tagaagaacc cttaagagtt caagccaaaa taagatattc agcaaaacca tcaccagcta
    12121 ctattcatag agtaggagaa gatactatta aaatagtttt tgatgaagct caaagagcta
    12181 ttactaaagg tcaatctgta gtaatgtatg atggagatat tgtagtaggt ggaggtataa
    12241 tcgagaaaag tttataaaaa catctaaaaa attaaaaaaa taagcaataa agtatattaa
    12301 gagtaaataa agtttataaa ataa
//
LOCUS       DAMJYX010000099        12008 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJYX010000099 DAMJYX010000000
VERSION     DAMJYX010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.102"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            48..866
                     /gene="larE"
                     /locus_tag="PC479_002874"
     CDS             48..866
                     /gene="larE"
                     /locus_tag="PC479_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2805403.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            921..2069
                     /locus_tag="PC479_002875"
     CDS             921..2069
                     /locus_tag="PC479_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2805404.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            2205..3593
                     /locus_tag="PC479_002876"
     CDS             2205..3593
                     /locus_tag="PC479_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2805405.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            3622..4320
                     /locus_tag="PC479_002877"
     CDS             3622..4320
                     /locus_tag="PC479_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805406.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            4323..5756
                     /locus_tag="PC479_002878"
     CDS             4323..5756
                     /locus_tag="PC479_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2805407.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            5778..6515
                     /gene="truA"
                     /locus_tag="PC479_002879"
     CDS             5778..6515
                     /gene="truA"
                     /locus_tag="PC479_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2805408.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    6626..6833
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7086..8285
                     /locus_tag="PC479_002880"
     CDS             7086..8285
                     /locus_tag="PC479_002880"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2805409.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            8532..9578
                     /locus_tag="PC479_002881"
     CDS             8532..9578
                     /locus_tag="PC479_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2805410.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            9686..10849
                     /locus_tag="PC479_002882"
     CDS             9686..10849
                     /locus_tag="PC479_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2805411.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKLNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(10938..11318)
                     /locus_tag="PC479_002883"
     CDS             complement(10938..11318)
                     /locus_tag="PC479_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2805412.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(11321..11980)
                     /locus_tag="PC479_002884"
     CDS             complement(11321..11980)
                     /locus_tag="PC479_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2805413.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         4831 a         1204 c         2100 g         3873 t
ORIGIN      
        1 aatgtaaaaa aataatatat agttttatag ttcatatttt tccagatatg gaggtaaatt
       61 ttgtgaagga aaaagaaaaa ttagataaat taaaaaaaat gttacttgaa ttaggcagtg
      121 ttgtagttgc ttattctggt ggagtagata gtaatttttt gcttaaggtc gcaaaagata
      181 cattaggtga aaatgttata gctgtgacta ttcatgcaat gatgcattca agtagggaaa
      241 ttgaagaggc taaacaatat actcaaaatt ttggtgttaa acatatcata ttaaatatag
      301 aaaactttga tttaaaagaa tttaaagaaa atggtgttga tagatgttat cattgtaaaa
      361 aatatatatt ctctaaaata aaagaggtag caaaagaaca taatataaag tacatagtag
      421 atggaacaaa tatagatgat ttaggagact atagacctgg gcttaaggca ttgagtgaat
      481 taggagtaat tagtccatta aaagatagta gtttaaaaaa agaagaaatt aggtcgcttt
      541 caaaaacatt aggattaaat acttttaata aaccatcatt tgcatgtctt gcgagtagga
      601 taccttatgg agttgaaata acagatgaaa atttaagaat agtagaaaag agtgaagaat
      661 atctatccaa tttaggtttt tctcaattta gagtgagaat gcatggagat atagctagaa
      721 tagaagttgg acaggaagaa ctaggtaaat tttttgaaaa taacaatttt aataaagttg
      781 atactaaatt aaaaatattt ggctttaaat atgttacact agatatgtca ggatataaaa
      841 tgggaagtat gaatttaaat gtgtagatta ttttaaaaat aaataagttt tatagttaat
      901 ataaggaaac aggaggtttt atgaaaaatt atacgttaat atcgccatgt ttttttggaa
      961 tggaaaaaat gctggctaga gagattacta atttaggata tgaaataata aaaacagaag
     1021 atggaagaat aacatataaa actgatgagt ttggaatagc aaagtcaaac atgtggttga
     1081 gatgtgctga aagagtacat cttaaaattg ctgaatttga ggcaaaaagc tttgatgaat
     1141 tatttgaaaa tacaaaaaga ataaactggt ctagatatat accttatgga gctcaatttc
     1201 ctatatcaaa agcttcctct ataaaatcaa aattatacag tacaccagat gtacaagcca
     1261 tagtaaaaaa ggctatagta gaaagcttaa agaaaagtta tttggaggat ggtttgctta
     1321 aagaagataa ggagaaatac cctatttttg tatttataca caaagataaa gtaaccattt
     1381 ctatagatac aacaggagat gctttgcata agagaggata tagagaaaag gcaaataaag
     1441 ctccaataag agagacttta gctgctgggc ttatttattt aactccctgg aaagcaggta
     1501 gagttttagt agaccctatg tgtggttctg gtactatatt gattgaagct gctatgattg
     1561 gtataaatat ggcacctggc ttaaatagag agtttatatc agaaaagtgg agaactcttg
     1621 ataagaaaat atggtgggat gtaagaaaag atgcttttaa taagatagat aatgaatcta
     1681 aatttaagat ttatggatat gatatagatg aagaatctat agatatagct agagaaaatg
     1741 ctgaaatagc aggtgtagat gaatatatag agtttaatgt aggagatgca actcaattta
     1801 aaagtgaaga tgagtttgga tttataatca ctaatcctcc ttatggagaa agattagaag
     1861 ataaagacag tgttaaacag ttgtataagg agcttggtta tgcatttaga aaattaaaaa
     1921 attggtcgta ttatttgata acttcttatg aagactttga atatgagttt gggcaaaaag
     1981 cagataaaaa aagaaaattg tataatggta tgttaaaaac taactttttc caatatccgg
     2041 gtcctaagcc tcctagaaat aataaataag ttttacatga tttatttgta aaatataaaa
     2101 ttttattaaa ataagtaaaa cattatgatg ccttgaaacg tctaattatt gtgaggagat
     2161 ttttgtgaac aaatttaaag atttatagga ttaggaggag ttaaatgaaa gtgttaaagc
     2221 ttattactgt agtagccctg atgatgttat ttgttactgg atgtaatata aaaactgaat
     2281 ctccagaaca attagcgaag atacctgact atgatgatac aaaaaaagta ttgtatgatg
     2341 gtattgacca gttactaaaa ccagattcat ctatgatatt gccttctaat acaaaagaag
     2401 taggaaaaat aaataaggta gatttaaaca gcgatggaat tgatgaatta gtagtatttg
     2461 aacaaaaaga agatttaagt aataatgtga gtcaggtagg gtttgtaacc ttgagttaca
     2521 atggagaaaa atacgtactt ggagaccatt ttttagaaaa tggcgaatca atagaatatg
     2581 ctaattttta tgatttggac tcagatggat ataaggaagt tatcttattg gttaaatcca
     2641 aagacaaaac gaatatgcat atatataaag tagtagataa tgaaataaca aaaatatatg
     2701 acttggatgc atcatggctt caaaataaag aagattttaa tgatatgaaa gtaaaaattg
     2761 gatatataga tggagatgac aagttagaca tattgatgct tcatttgaat aataagacta
     2821 atgaaatgtt tgcaagtgtt gcaaattttg atgggaaaat gaaaatcaaa gattctgtta
     2881 agtttgaaaa tgtaaaaaac ttaagtgaat tatatataac attaggtaat gttgcttcat
     2941 ctgttggaaa ttcttctgca gctataaaag gtattgtact tgatattcct atattgaaag
     3001 ataataacta tattacgcag atactttata tgaaagatgg gaaaattaac aaagctttta
     3061 gtgattatga taaaactatt actaaatctt attatatacc tgtagatgat attgacaaag
     3121 ataagattat agaaatacct atagttgcag gcagtacagg taataataaa aatacttata
     3181 gttctaaatc atctgcaact ataagttggt atagatggaa tggaaaagaa ggtgctgact
     3241 caagtttaat atttacaagt cagatatatt ataattataa atataatttt aaattattta
     3301 ttccaaataa tttatttgat aaaatacttg ttgaacagga atttgttgga gaaaaagcag
     3361 tatttaaatt ttactatttt gactattcag accgtaagaa tttatttaat atagtagtag
     3421 agagtaaaaa taaacttgaa gatagaaaaa atacaggtac tcaaaattct attgtacttc
     3481 aagagagcga tgaatatact ttcttactag ttgtaaacaa tgcaaatgaa atggataagc
     3541 tagatatgac tatagacgca ttaaaagaat atttctcatt aatatatggt taatattata
     3601 ctcaattgga ggttttattt tatgaaggaa aagatactta tactagaaga tgaaattggt
     3661 attagaagtt ttgttagtat aaacttaaaa agggaaggct atgaaatagt tgaggcaggt
     3721 actggaagag aagctataga aaaaatgaca acagaaaagg atattacaat agctctttta
     3781 gatgtaatgc ttccagatat aagtggtata gaagtatgta aatttataag ggagaatttt
     3841 gaccaagtag gtataataat gcttactgct aaagctcaag aagatgataa aatagaagga
     3901 tttatctcag gagcagatga ttacatcata aaaccattta gtataaaaga gcttttagtc
     3961 agagtttctg ctcttcttag aagagtagca aaggatgatt caagtgttaa gtcgagtgag
     4021 atagtttctc caccatttat acttgatata gacaaaagaa aattatttaa aaatggaaag
     4081 gaaattgagc ttacaccaac tgaattttca atagtgaaat atctaatatc aaatgctaag
     4141 cagtcgttaa gtagagacca gatattagac gaagtatggg gaactaacta tttatatgat
     4201 tttaaaatag tagatgtaaa tataagaagg ataagaaata aaatagaaga tgacccatca
     4261 aaaccaaaat atatacaaac tatttggggt tatggatact gttttagaaa ggaagaataa
     4321 tagtgttaca ttttgaaggt ctgagaaaaa aagtaataaa aaactatttt ataataatta
     4381 ttataatggt tactctgttt gaaggattgt tcatgtttta catacaaaac tactattatg
     4441 attctgtcaa acagctactt gaatctgaaa taaaatatgc tgatgaatat aatgctatta
     4501 caatggaaac tacaagtttt gagaaaaaag taaaaaatat atttgataaa caacccttaa
     4561 ctaaaaattc agaatttgga atttcaataa tagataaaga taaaaatatt atattagacc
     4621 aatatggttt taaaagtaaa gaaaaagcaa actatgaaga tgttaacaat gcattaaaag
     4681 atataaaaac taaaaattta acaccttata cttatagaat accagataca ggggagcatg
     4741 ttatgagtat ctccttacca cttaaggtaa ataatataat agaaggtgtt gttagatata
     4801 cagtatcatt agatgctatt gataatgcaa tattaaagca agctacatgg cttatattag
     4861 caggaatatt tatacttatt atagctatac taataagtct taaatttgct gagactctta
     4921 taaaaccatt acgagagcta aagaaatttg caaatgaatt ggctgtagga aattataata
     4981 taaaattaga aaagatgaag attgtagatg atgaaattgg tgatttagca cagacatttg
     5041 aacacatggc acatgagatt gacaaaagtg aaaaattaaa agaggaattt atatcatctg
     5101 tatctcatga actaaggact cctcttacat ctataaaggg gtggagtgaa accttaggtt
     5161 atgaaagtat aacaagagaa gaactagact taggtttagg cattatacaa gatgaaacag
     5221 aaagacttat aaaactagtt gaagaattat tagacttttc aaggctttct tcagacagga
     5281 taaaacttca tgttgatata gttgatgttg aaggtttaat tgtaggtgtt gtaaatcaac
     5341 ttaaagttaa agcagctgaa aaagatatat cacttttatt tgaatttgaa aatgaattta
     5401 tagagaacat tcaaggagat aaaaatagat taagacaggt tctcataaat ttaatacaaa
     5461 attcatttaa atttacgagt caaggtggtt acataaaagt tgtagcttct caagatgaag
     5521 agataacaac catatcagta gaggataatg gttcaggtat agaaaagcaa aatttaaata
     5581 aagtgttaga taaatttttc caagaagatt ataataaggc tggtagtgga ttaggactag
     5641 caattagtaa tgaaattgta aaacttcatg gtggaagaat gaaaatagaa agtgaaaaga
     5701 atgttggaac aaaaataact tttaatataa aaaataaatt tgctaaacaa gcataaattc
     5761 ttagaaagag gattaagatg agaaacataa aaatgatagt agcatacgat ggttcaagat
     5821 ataaaggata tcaaaaatta ggtgacaata atatgactat tcaagaaaaa cttgaaaatg
     5881 ttttaagtaa aatgactaat gaaacagtgg agataatagg ttctggaaga actgatatgg
     5941 gagctcatgc aagaggacaa gtggtaaatt ttagaactaa ttgtatggat tctttggata
     6001 aaattcaaaa atatttatat gaatatttac cagaagatat agttgtaaaa actgttgaag
     6061 aagttgatga aagatttcat tcaagataca atgtaaaatc aaaaacttat atgtataaaa
     6121 ttgacaataa taagtatcat agtcctttta taagaaaata tgctactcat gtaagtaaaa
     6181 aattagattt agatagaatg agaaaagcaa gtgagtattt agtaggagaa catgatttta
     6241 ccagttttgc ttcttctaaa tctaagaaaa aatcaaatgt aagagaaatt tattctataa
     6301 atataaaaga agatgataat gttatagaaa tatatgtaga aggaaatgga tttttgtata
     6361 acatggtaag gataatagta ggtgctttaa tagatgttgg tctaaaaaga agagctccac
     6421 aagatattaa gtatatgctt gaatcaaaag atagatgtca atcatcagat actgcaccag
     6481 ctaaaggact atgtctttgg aaagtaacat attaatcaat tatataaaac tctctttaaa
     6541 ataaaaataa aataaattat atagttgaca taaatggaaa aataaactat tatatatttt
     6601 ataagagaga tataaaactt tatatttaaa tcctgtgaga aagagagtaa cttctatgtg
     6661 ttttatagag agctgaggat ggtggaagct tggcaaataa gtattagtga aaagaacttt
     6721 ggaggatact aattgaattt agtatgttag taggtaatct cgccttaaga gaaagagtgg
     6781 ataagtttat cttatcaatt agagtggtaa cgcggatata tttcgtctct tggtttttcc
     6841 aagaggcgtt ttttaatata tttaattatt agtgtttatc tggtatgttg aacatttact
     6901 aattgattag ctaagtttat caataagagt ggtaccgcgg atatttcgcc tcttagtatt
     6961 ttaaaatact aaggggtttt tttagtatta aaatgatttt atgtgagtct aaattctcag
     7021 agtcattcaa tattaagaat ttctataaaa taaaaattat aatttaagat tgggaggaat
     7081 taaaaatgaa ggaaaaagta gtattagcat attcaggagg acttgacaca tcaatcataa
     7141 taccttggtt aaaggaaaat tatgaagata tagatgttat agctgtttgt ggaaatgtag
     7201 gtcaggaaga taaaatggaa gatgtatatg aaaaagcttt acaaagtgga gcatcaaagg
     7261 catatgtgga tgatataagt gaagaatttg taactgaaac tatatttaaa gccgtaaaag
     7321 cagaggctaa atatgaagga aaatacttat taggaacttc tttagcaaga cctataatag
     7381 ctaaaaaatt agttgaagtt gctcataaag aaggtgcaaa atatatatgc catggatgta
     7441 caggaaaagg taatgaccaa gttagatttg aagctacaat tgcagctctt gacccgacta
     7501 taaaagttat tgcaccttgg agaatttggg atataaaatc aagagaagat gctatagact
     7561 atgctgaaaa acataatata aaggtaacag caactaaagc taaaatatat tcagtagatg
     7621 ctaacctttg gcatgtttca acagaaggtg gagatataga gcacctagaa aatgaacata
     7681 aaaaagatgt ttataaacaa tgtgtagacc cagaagatgc atgtgatgta gctgaatatg
     7741 tagaagttta ctttgaaaaa ggtgtaccta aaaaggtaaa tggtgaagaa ttatctccag
     7801 ttgcactgat tcataagtta aatgagttgg gttgtaaaca tggaattggt gttatagata
     7861 tagttgagaa tagacttgtt ggtatgaaat caagaggtat atatgagact ccaggaggaa
     7921 caatccttta tgaagcacat aatattttag aaagtgctac tttagacaaa gatacattac
     7981 atttcaaaca aatggtatct tataaatacg gagaacttat ttacaatgga ctatggtatt
     8041 gtaaattaag agaatctata gatgcattta tggaacaaac tcaagataat gttacaggaa
     8101 cagtaaaagt taaactttac aaaggaaata taaaacctgc tggaatattt actgaaaatg
     8161 ctctttatga tgaaggaata tcttcttttg gaaatagtga actttatgac cacaaagatg
     8221 cagaaggatt tattaattta ttcactttac cattaaaaat cagagctatg aaagctggaa
     8281 aataataaaa taaattattt aagagtgtat aaaaactatt tttaggtttt tatatgctct
     8341 ttttttattt gaatttatta acttatacag taaatagtat aaaaaaatat attatagttc
     8401 caaatggctt aataatgtga aaatataaga cctaaaatat aaattttata aatacaatag
     8461 ttattgactc tctcattgta agagggttta tactgaatct aatgaaaatt tatagtgagg
     8521 agatagatta tatgagaaaa ataattgaaa ttaaacatca ttcacatgat tatgagtgta
     8581 tgtggaatgg tatagaagat ttatatataa ataaaaccgg tgaatgcctt ccaaacgatt
     8641 ttttcttttt actgtcaggt tttggttcat tttgttatat gaaaacaaat aaagctgatt
     8701 tgaaaagaat ggtagctttg ggagatggaa ggacaaaaca gatgtataaa tttttagcac
     8761 caattgttgg atttgaatac aaacatcatg agtttaaaaa gtatgagcaa gcattaaaga
     8821 aagcaaaatc tgaaattgat gatggatatc cagttatttt aggagcttta gatatgtatt
     8881 atctaccata ttataagaag ctatatcata aagaacatat tccttttcat tatatattaa
     8941 tggtaggata tgatgatgaa ggaatatatc tgtatgattg tggtagaaca gaattattac
     9001 atctttctta tgataaatta cgtgaaagca tgaattgcaa ttcccctgga ttaagcaaag
     9061 aaaatactat ttgcacaata agaatgaata cttcaaagaa taaatataac attgctagag
     9121 aagctattgc tataaagcgt gatatgtttt taaatccacc taaaggattt ttgggatata
     9181 aaggttttga aaagtttatt aaagagttgc cacagtggaa cactgaattg gataaagatg
     9241 attatgataa gattttgacc aatatggtta tgttttttgg aacagtacca acaattccaa
     9301 atgcaatcaa aggaattaaa gaaccagata ctgttcaatt taaaggtgga tttgataaaa
     9361 tgagtagaat gctcaacagt attggggagg aatacaaaaa cgactctttt atacaagtag
     9421 ctgatatttt tgataaaact gctattataa ttgagaaaat atccaatatt ataatagatt
     9481 acttaactca aacatgtgat gatacaggac aacttccgtt attgttctct gaggtcttag
     9541 agcatatgaa aacaggatat ttaatcttgg atgtataaaa aatatattgt tgaccctaac
     9601 attacgtaat agtgtaatat gaaaatatag cttgaatata ttagtaggta ttgatggctg
     9661 taaaattaaa atttgaggtg tggatatgag aacagtgaaa caagtttctg atttaactgg
     9721 aataagtgtg agggcattac attattatga tgaaataggt cttttaaaac taaataaaat
     9781 tacagatgca ggatatagac tttatgatga tgaatctatt aaaactcttc aacaaatttt
     9841 attttttaag gagattgata ttcctttaag agaggttaag gaaataatgt caagccaata
     9901 ttttgataag gttgaagctc taaaaaatca aaaaaaatta cttattttaa aacgaaagag
     9961 actggatgaa ttgatagagc ttataaatca aactctaaga ggagaaggaa atattgattt
    10021 taaagagttt gatatgagcg agtattttga tgttttagaa gaatttaaaa gagagcatag
    10081 gaataaggta attaaaattt atggtagtgt agaaaaatac aatgaatata tagaaagagt
    10141 taagtctaat gaagagaaaa ttgctaaaat ggctataaaa caatatggaa ctattgagaa
    10201 gtttgctaaa gctataaaaa caaattttag cagtgatata ttgaatttag gagaaaaatt
    10261 tgatagatat aaaaatgatt gtttaaaaga aaaacatcca aaattaaaag aattatatag
    10321 aaagcttgta gaagatttaa ataggaatcc ttcttcaaca gaccttcaag aaatagcaaa
    10381 agagataaca gatatatcta aaaaagatta tgaaattttc tctatggata ctggtgatga
    10441 caactggtat tatatggttc aaaattatct agtaaatcct atgtggatag aagaagtaga
    10501 taaaaagtat ggaagtggag caggtaagtt tattggacaa gtactaaaga cttatctaag
    10561 agatagaaaa ccaaagataa atacattgta tgaaaaactt gtagaagatt tgagtagaga
    10621 ttgttcttca agggaagttc aaagcattgt tgaagaaata gacaatgaaa tgaaaagaag
    10681 taatgaattt tataaaatag acaatggtga aagatacttt gattatatgt cagagcttta
    10741 tttacaagat tctaactata taaaagtaac tgataagaat tatggtgatg gtgcatctaa
    10801 gtttattgga gaagctttta agatttattt tgataataat aattgttgaa aatttggaag
    10861 taaaaaatat gcaagtttta taaaagcaca aaaataagga tgtagattag ctccatctat
    10921 atccttattt aaagttatta aaatatatag tttacatcaa acttaacact ttcaataata
    10981 tttcccaaat atgtttttat actttcaact tcttctaaag attttaattt attttcagta
    11041 taaattgctt tgtccagaat attaaacctt aatcctattt catcaaagta ctcatgattt
    11101 aaaactttgt accaatggtt tttttcttta ttccaactcg tagatattga atcagcttct
    11161 ttttgagctt tttcccaatc ttcattttct atagattttt ctaaaacaga tatattgtct
    11221 ttatatcctt ctgtaaaatc atatagttta ttgttaacat aaaatccaaa taatacaaat
    11281 aagatagtcc atagtataac aaatgttgta gatttcaata ttaatcaccc tttttatttt
    11341 gaataaatat tttgtcattt tcatctaaaa cacaaataag aacatcttta aaagtttcta
    11401 tgtgattaga ttttaaaata cccattaacc agttagtatc tttttgagct atgtctagat
    11461 ttttatttat tattctacca tccagtataa gtgggatagg taaatgatta aaagctcttg
    11521 gaggagtact attgtagctt gatgctggta caacacttag attaccatca gtctccaaaa
    11581 tagcatattg tacatctttt aggttaaagt atccttgaat cctaagttgt tctaataatt
    11641 catctatact aattctttct tttttaagct ctttatatac aatttttcct ttatctatta
    11701 atacagaagg tttacctgat aaaaatgaac ttaattttct actttttaat gataagaatg
    11761 aaattagagt ctgcatgatt acaagtccag atattgctgc aacaccattt atgattggta
    11821 tattattatt ttccattggg acagatgcaa cttctgcaat taatagtgtt ataactaaat
    11881 caaaacagtt catttcagct atttcacctt ttcccatgac tctaagtgca attagcactg
    11941 ttatgtataa tataatactt cttattaata ctactgtcat atctatcaat tactccttta
    12001 aaaaatat
//
LOCUS       DAMJYX010000100        11873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJYX010000100 DAMJYX010000000
VERSION     DAMJYX010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.103"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(1..94)
                     /locus_tag="PC479_002885"
                     /pseudo
     CDS             complement(1..94)
                     /locus_tag="PC479_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            389..910
                     /locus_tag="PC479_002886"
     CDS             389..910
                     /locus_tag="PC479_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2805414.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            1289..2464
                     /locus_tag="PC479_002887"
     CDS             1289..2464
                     /locus_tag="PC479_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805415.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            2499..4037
                     /locus_tag="PC479_002888"
     CDS             2499..4037
                     /locus_tag="PC479_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2805416.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            4069..5271
                     /locus_tag="PC479_002889"
     CDS             4069..5271
                     /locus_tag="PC479_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2805417.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(5438..6037)
                     /locus_tag="PC479_002890"
     CDS             complement(5438..6037)
                     /locus_tag="PC479_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2805418.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            6207..7565
                     /locus_tag="PC479_002891"
     CDS             6207..7565
                     /locus_tag="PC479_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805419.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            7558..8190
                     /locus_tag="PC479_002892"
     CDS             7558..8190
                     /locus_tag="PC479_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2805420.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            8519..9676
                     /locus_tag="PC479_002893"
     CDS             8519..9676
                     /locus_tag="PC479_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2805421.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(9823..10458)
                     /gene="lexA"
                     /locus_tag="PC479_002894"
     CDS             complement(9823..10458)
                     /gene="lexA"
                     /locus_tag="PC479_002894"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2805422.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(10562..11689)
                     /locus_tag="PC479_002895"
     CDS             complement(10562..11689)
                     /locus_tag="PC479_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2805423.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         4454 a         1458 c         1923 g         4038 t
ORIGIN      
        1 tagacgtatt ttttctatat ttactgggag acatattata ttttttatta aataatctaa
       61 taaaatgctc tacatttgga taaccaatac tattttactg ataatctttt ccttatcact
      121 aatcttacat tttaaaatat tatttttact cgtaatatta tcataagaat ttgtttctta
      181 tttaaaacta attagaatta taaagtttat tgccatgtta ttaataaaat aaatccagtt
      241 aaatcgagtg ctagttaaag taataagaaa cttttacaat ggtttccttt atcgattgaa
      301 ataaaagtta atatggtata atatctaaag agagaatctt atcaattaga atatgcatag
      361 aaacaagtgg cttaaaagga gacgttatat ggaagagata gtaaagaagg aacaagcaag
      421 tatgaatgat atttttttgt atcttaaagc taaatcaaac tcattatata aatttgtaat
      481 attatataat gagtttatca ataaacctaa agattatgga actggggaac ttataaacat
      541 ggttgaagta catatgctaa cagcgattga agaaaatcca ggaataagtc caactcagtt
      601 agctaaaata tggaacagaa ctaaaggtgc aatatctcaa actatatcta aattggagaa
      661 aaagggttat ataactaaag aaaagaaaga aggaaatgca aaaactatat tactatatcc
      721 aaccacatca ggagtagagt taagtaaggc acataaacta tatgacacca tagatgttac
      781 aagaacattt gaatacttaa tcaaggagtg tacattagag gagatagata gtttttataa
      841 agtaattgat ttgtttatta atttattaga agaatctccg caggaaaata atgattctga
      901 aaaagtgtag ctgagattga agttgaataa agagaaatag atattataga agtagtactt
      961 aagtacacat tatataaaaa tataataggt ataggattaa aaagtttagt ggtttaaagt
     1021 ttaaccacta aactttttta atgtctataa tctactatta tttaatttac taaaaataaa
     1081 aaaactcatt atattaagat aaaaatagaa aaataactag aaaaatacta taatatatca
     1141 gcttaataca gaaaaaagtt aaaaaatttg agaaaaataa aaatgaaaac gttttatatt
     1201 gacatagaga aaaagtagtg ataccattta aatatagttt agtagctaaa ctatatttaa
     1261 attataataa ctattttagg gggatgttat gaatttaaaa caattaagtg aaaaatacga
     1321 agaatatgta attcaaatga gaagagaatt tcataagtat ccagaattat cttttaaaga
     1381 aataagaaca acccaaagaa taagggaaga attacaaaaa ataggggtcc cttttgtatc
     1441 tattgaacca aatatcgtaa tagctactat acaaggcaaa aatgaaggta agacaattgc
     1501 aattagagga gatatagatg cgcttccaat gcaagaagat tcagatgttg aatacaaatc
     1561 agaatatgat ggaataatgc attcttgtgg acatgatgct catggagcta tgttactagg
     1621 acttgcacat atgctaaatg atataagaaa agatttaaaa ggtagagtat tattagtttt
     1681 tcaagcagct gaagaagttg gtggtggaca caaggagata atacagtact ttaaggaaaa
     1741 tggaggacca gatagattaa tagctactca tgtttggtcc gatattgaag ctggtaaaat
     1801 ttctgttgaa ccaggacctc gtatggctgg tgggtctggt tggagaatag acataacagg
     1861 tagaggaggt catggttcta gaccagatca aagcatagat cctataaagc cagcttgtga
     1921 tatagtttta aaagtatctt ctataccagt taatcatata agtgtactag aacaatgtgt
     1981 tgtacatgca tgtgcaatac atggaggaac tacaatagga aatgttattc caaacaatgc
     2041 agaggtaata ggtggatata gatattttac tgaagaatca ggcaaaaaag tgtttgacat
     2101 tataaagcaa atgagtaatg gtgttggaca agtttatgga gtggatgtga aggttactca
     2161 tactggaggc ataggacctg ttatcaatga tgaagaagca gttaagatgg ctaaagaaat
     2221 agtttctaat atagatggat tggagttaga ttcttatgag ccaatatgtg catcagattg
     2281 ttatggtgaa atattaaaag agtattctgg cttttattgt tatctagggg taggtaacaa
     2341 agaaaaagga ataatatatg cacaacacca tcctaagtac aatatagatg aagatacatt
     2401 aaagttaggt tgcgaattta tggctaaata tgcagttgaa tttttaaata aaaataataa
     2461 ctaaaataaa tcgaatttta taaatggggg ggatgaaaat gcaaggtgca ttaaaggaaa
     2521 ataagaaaaa aagtggtgga ggatttttag gatggattga aaaagttggg aataaaatgc
     2581 cacatcctct agcattattt acatatatta cattgatagt agtagcatta tctgcaatag
     2641 caagcttttt aggtttttca gcaacaagtc ctgctacagg aaagttaata aaagttatga
     2701 acttaataag tgcaaaaggt ttagtactat ttatgcaaga gtttgttaag aacttccaaa
     2761 acttccctgt gcttggagta gttttagtta tggcagttgc cacaggagtt tgtgaaaaga
     2821 caggattttt ttcaactgcc ataaaaatga gtttatcaaa agttaagggg aatgcggttg
     2881 tatttataat agcttttata ggagtatttg ctaatcaagc tggtgatgct gcttttgtat
     2941 tagtaccaac tcttgcagga gcaatatttt atggattaaa tagaaatcca ttagctggta
     3001 tattctgtgg atttgcatca gttggtggtg gatttgcaac agctgtaatt cctgggggat
     3061 gggacgttac gctaacgcca ataactgtat cagctgctca aactattcaa cctgcatttg
     3121 atatgacatt attagctagt tattatgcac tatttgtatc tgcgtttata gtagctacag
     3181 tatcaacaat agtaacggtt aaatttatag aacctaaatt aggtaagtat actggtaaac
     3241 cagaaggtat agatgataat caaggttttg aggtcacaaa agaacaagca aaagcagcaa
     3301 agttagcagg ttttacagtt ttagcttatg tagcgttatt aattgctttg tgtataccag
     3361 ctaatagttt tttaagaagt ccagaaggtt ctttaatatt tggagcacca cttatgtctt
     3421 gcttacagtt ttttatagta atactattct tcttgccagg gattgtgtat ggaatgaggg
     3481 ttggtaaaat caagactgta aaagacttaa atgatatatt atgtcaatca atggcagcaa
     3541 tggcaccgtt tatcgtactt gcaatagtta taggtcaatt cttaacgcta ttctcagtgt
     3601 ctaatttagc acaggtacta gctataaaag gtggagcatt actcaactca ttgccactac
     3661 caccacaagc tataatactg ctattcttag tactagtagc gtttataaat atatttgtta
     3721 taagcggaag tacaaaatgg atgatatttg gaccagtatt tgttccaatg ttaatgcaat
     3781 taaacataca tccagcattt actcagtttg tatatagatt aggagattct gtaaccaatc
     3841 atttatcacc attaaatgca ttctttataa tacttttagc aacagtccaa aaatacgata
     3901 aaaatgcagg tatgggaact atattctcag ctatgatacc ttacactata ggttactttg
     3961 tagtacttgc agttttagta gtagtttgga tgacaatagg tataccagta ggaataggtg
     4021 gacaagtttg gttataaaac atatttaata attttgggag gaaagaaaat ggaaacaact
     4081 gaaataagat acaattttga tgaagtaata gatagaagtg gaacaaactg tcttaaatat
     4141 gatcttaaga gtcaatttat gccagaatct ccagaagatg cactaccact atgggtagca
     4201 gatatggatt ttgcatgtgc ccctgagata attaatgcaa tgcatgaaag aatagacaga
     4261 aaaatatttg gatatagctc tcaacaaact gatgattatt atgaagctgt atgtggatgg
     4321 tttaagagaa gatttgattg gaaaatagaa aaagagaata tattctttag cccaggggtt
     4381 gtgcctgcgt taggattttt cacacaaata ttaacacaaa agggtgatgg tatcataata
     4441 gaaaagcctg tatactatcc ttttgaagat aaaataacta ataatgaaag aaaagtagtt
     4501 aatagtcctt taaagtttga agatggtcat tattctatgg attatgatgg atttgaagaa
     4561 ttagctaaag atgaaaataa taaggtgttt atattatgta gtcctcataa tccagttggt
     4621 agagtatgga aaaaagaaga gcttagaaaa atagtagaca tatgtaagaa atatgatttg
     4681 tggataatat ctgatgaaat acattgtgat attattcgta aaggtgaaaa gcatatacca
     4741 ttagaagtag catgtcctga ttataaagat agaataataa cttgtacagc tccaagtaag
     4801 agttttaact tagctggtat gcaattatca aatatagtaa tcaataataa ggaattacaa
     4861 aaaaaatgga tgcttgaaac agattctaaa ttagctatat tcctacctaa tccattcgca
     4921 atagttgcta ctaaagttgc ttataatgat tgtgaagatt ggatgaatca agtaaatgag
     4981 tatatagatg gtaatattaa atatgtagaa gattttgtta atgagtatat gcctaaagta
     5041 aagataataa aatctcaagg aacttactta gtttggttag actttagagg gtatggatta
     5101 gatgcaaaac agcttgaata tgtaatgctt aacaaggcga aggtattgct agatgaagga
     5161 tacatattcg gagaagaagg agcaggattt gaaagaataa atgttgcttc acctagaaca
     5221 attatagaag agtgtatgaa tagaataaaa tgtgcatttg aaggattata atagtaatat
     5281 ctaaataata tatttgtgta ttttaaaaaa agactatgct aaaaaaatat ttcaaagcat
     5341 agtcttttaa agttttatga tgagtttaaa aaatttcaaa acgttactat tcacttatat
     5401 gtttatatta gaaataattc cacaatatac tcattttcta aagttgcact tttatacacc
     5461 ttgcttcacc aatatcatgt gtaaccaaaa tagtagactt attctcattc ttaataatac
     5521 tataaacatc atcacaaact aataatctag tctgataatc aagagctgaa aaaggttcat
     5581 ctaaaagtag aatatctgga tttacagaaa gtgttctaat aagcgcaact ctttgtctca
     5641 tacctccaga taattctttt ggatacatac ttctaaaatc ccataatcca taagtcttta
     5701 aaaggctttc aactctatct aaagattttt tatctttttt gccttgaatt tccaatccaa
     5761 tagttatatt atccattata tttctccatt caagcaaatt atctttttga aacatatatc
     5821 caatatttcc agtaatcttt atatcaccac tagtcggttt taaaagatta gtaagaatat
     5881 ttaatattgt agatttacca cttccagaag gaccaagcaa agttaatatt tctccttctt
     5941 tcaagttaaa attgacatcc tttaatactt caagctcgcc ttccttagtg tagaagttca
     6001 tatttatatt tttaacttct actatgttgt cattcattat gccaccccct ataactttta
     6061 gctcgtatat attatgttat taacaatatg acctatttgt accatttttc ataaatataa
     6121 tataaatatt ttgtttattt acgtattttc ttagtaactt gtacgtataa attatagata
     6181 aataaattat aaggtagggg ataaatttga gtaacataat aacgaattta atagagtcaa
     6241 tatttactaa tccaataact atattgtttt tacttgcaat aactgtgtat tctgttttta
     6301 aaataataga aaataacaga gtatattcat ttgtaagttc aaaattagac gaaatcaata
     6361 gagactataa gacaagtgag ttttataaaa aggtaaggga tgactactat acatatagta
     6421 aagaaaatcc ttatgcagac gtaaatataa cttcatttat agaggaagtt gtatctgatt
     6481 taaagcataa taatttgcca ttgttagaaa aaattagaag tataaaaaat tcatcttcaa
     6541 taagtatatt acttggggtt ttaggtacgt ttgttggtct ttctacaatg cttttatgtg
     6601 ttgatactaa agacattata aattcacttc cttcaactat tagtagtatg caaactgcat
     6661 ttacaactag tatatttgga gttgtatttt ctctaattat aggttacttt actaagataa
     6721 aagattgtga acatgttttg atacaaataa tgttaaaatc tgaaaattta ctaacatctg
     6781 aaataactca ttttaagtca gaaagaatgg atttaaaagt agaagaggta aaaaatacaa
     6841 taaaacaaat aagtaagtct atagaagcta ttgaaaaatt tgataaaata tctaaagatt
     6901 taaatgactt taatgatgaa tttatcagtg gtatagaagc tttaaaaagc cttttagaag
     6961 gttctcaaag ttctattaag acatttgacc agagcgtaag aaaattagat aaacagttta
     7021 atattttaaa tataaaattt gtaaagcttt ttgataaata tgataatcaa gataatataa
     7081 ataaagagat tttatttgat ataaaagaat cttcaaaaaa tatatacaat gccactgaaa
     7141 gccaattcaa aattagagat tatataaaaa atataaatgc aggatttgct ctatatgaaa
     7201 gaagtgctca agatttatta actaaattaa tgacccatga aaataaaata tctcaaaatc
     7261 aaaaaatcct tttagatgaa aagtttacct tagatgactc aataaaaaat ctctcaagta
     7321 ttattgaaaa cttttctaat gacctacaag ttaaactgga tatgatgttt gaaaattctt
     7381 tagatattca agataagtta gatgttatgt ttaataactc ttttatgaat gatgaagttc
     7441 ctcttgatag tgaagaatta tttaaggatg atgttaataa tgtatttaat ccttttagtg
     7501 aagaaattta tgagatagag gataaagaaa taaaggtcat aggtgaggat gagttaaatg
     7561 aataataaat atagaagaac tgttaacaga gaatttgaaa agagtagctt ttggccaaca
     7621 tttacagatt tattatctac tgtattgatg gttgtgattc ttatcttatt tagctcagaa
     7681 agtatttcag gttctgttga acaagattta gctaagaatg tcaatgcttc tgttgaagaa
     7741 acttttaaaa aaagtggaat acctgtgaag gtagataaat ctaatgggca agtaactttt
     7801 ggtgaaaaaa ccatgtttga tgtagatagt gatgttttaa aaccagaggc taaagaaatg
     7861 ctaaaaatgt ttgttccaaa gtacatagag actatttata aagattatgg tgactatata
     7921 tccaaaatag ttatagaagg tcatacagat gatgtgggaa gttatatata caacttagat
     7981 ttatcgcaaa gaagagcata tagcgttgct aaatttatag tgggagatga aataggtgac
     8041 tataaataca aagataaagt gactaagcat attattgcaa taggaaggtc aaaagctgaa
     8101 ctcatcaaaa atggtgacaa tagcgtgaat agagatgcat ctagaagagt agaactaaaa
     8161 tacgaaataa atattaatca aaataaataa ttaaattttt ataaaaaaaa agaaggcagt
     8221 tggtgtgagc tttaagcctt ctttaaggaa aaagtttatt tatttttgtt gatacttaac
     8281 atattatagt ttttagaagc agtttgcaat atattaaacg taaatggtcg tttaaaatgc
     8341 ataactggat actactgatg taaaatattt cattttatat aatataaaaa ttatacagtg
     8401 tcaattttct atattattgt actataatct attatttaaa atattatgta aaaaaatatc
     8461 ataaaaacgc atatcatgtt ataattacta gagtattatg aatatggagg cgtagtttgt
     8521 gatagaaata agtataaaaa ataaaaaaaa ttctaaaatt aaaactttga tacttttatt
     8581 gatagtatct atatcgattt atttaactgg ttgtactaca tcagataatg gaaataatca
     8641 atcaaataat aatgattctt tgtctagtag tatatcaagt gataagaatg taaaaattca
     8701 ttttattgac actggaaata gtgatgcaat attgattcaa gatggtaaaa cttttacatt
     8761 gatagatggg ggagataatg atgatgaggg attaatggta gattacctaa acaatcaagg
     8821 tgtaaaagat attaaatatc ttatagctac ccattctcat gcagaccatt taggtggact
     8881 tgattctgtt gtcaaaaact ttaatataga aaatgttttt gtatcgaatg gttcagcaga
     8941 aacaaagagt tatagggact ttataaatgc tttagcaaat aagggtttat ccccaagtgt
     9001 accgcttgaa aataataagt tctatttaga agattcttat tttgaagttc taaatacaaa
     9061 tgggggagat actactaatg aacaatctct agtccttgta tatactaatg ggaatgataa
     9121 agttttattt acaggtgatg ctgaagaagg taccgaaaaa gaaatactac ctaagcttga
     9181 gaaagtagac cttttaaaag tagctcatca tggttctcgt agtagttcat ctcaagagtt
     9241 tttagataaa gtaaatcctg aatatgcggt tcttttagta ggcaagggta attcatatgg
     9301 tcatccacat caggagacaa tgaataaatt aaaaaaaatg ggagtaaaag tgcatagaag
     9361 tgatgagtgt tcagatataa tatttgaatc aacaggtgat ggagtattta caagttgtaa
     9421 agatggaagt tataataaag gcgttagaga agatggaaac tctggaaaat caaacagtaa
     9481 tacaagtaaa aatgattctt ttaaaaataa acaaaataca tccaattcag aagaaatagt
     9541 gtattttact ccaaaaggta agagctatca ctctacaaaa aattgtagtg ggctttcaag
     9601 aagtaaaaaa atattatcag gcacaattgc agaaagtaag aaaaatgacc catgtgatat
     9661 atgttatgga aaataacaaa aaaacaacca atttatctat aaagcttctg aactttaaat
     9721 catttaaaaa ataaaataaa tgcataaaaa aataagctat aatcaataga taacacattt
     9781 tatctatacg aatagcttat ttttttaagt attttttaat agctatttaa taactctaaa
     9841 tacaccagtt acaataccta caatttcaac ttctgagtca ttgagcataa ttggttccat
     9901 aaaatcattt tctggctgaa gtcttattaa atttccttct ttaaaaaacc tcttaaccgt
     9961 agcagattca ccatttataa gtgcaactac tatctgagaa tttaaggctg tatttttttt
    10021 gtctactaca acgtaatctt catctaaaat tcctgcatta atcatacttt cgccttttac
    10081 tcttaataca aaattgttac tgccctttac taaactggca gggaatggaa tgtattcctc
    10141 tatgttttgt tgagctaaaa tgggttctcc agctgttatt tgacctacta aaggaagctc
    10201 tattatttct tgattagctc cagaaacatc atttacttta ctacgttcta aaacttcaat
    10261 agctcttggt ttagtagggt cctttcttat gtatcccagt ttttcaagtt tatttagatg
    10321 agagtgcaca gttgaagtag atcttaaccc tacagcagta catatttctc ttacagcagg
    10381 tggataacct tttaatatga tttgagactt tataaattcc aatatcaaga cttgcttttc
    10441 agttagatct aaatacataa catcactcat ttccaaacat attttgtttt tatttccata
    10501 cactttatat ttttaattat aacataatcc atttatatct gcaaacattt gttcctgatt
    10561 tttaatcgtc ctcaatccat tcaaaattct tgactatatc acgttttaca tttttcttat
    10621 gagctgcata aagctgagta gttgtgacat tatcatggtc aagtaagttt tgaacatcat
    10681 atatagaaaa tcctgtttgg attagagaag ttgcagccgt tgctcttaat ttatggggac
    10741 tatatccatt gtctctagta gtatcaagtg gaatagatgt atattttttt accagtgttc
    10801 ttatagcctt tgctgtaatt ctcttatttt gaagagacaa aaataaagca tctttttctt
    10861 catcatttaa tagttcatct cttgttcttt cgttttgcaa ataatctttt attacatgtt
    10921 cacaagtatg atttataggc attaaaactt ctttcccacg ttttctatat attttaaatt
    10981 cacctcttga aaaattaaaa gaggagatat tgagttctct aagctcattt aatctaagcc
    11041 cgtatgtaac aaataatgct agtatagctt tgtcacgtaa tttagttttt ctccaatata
    11101 ccttttcttt ctctgtaagc ccttcacctg tttctactga ttcaagcatc ttagcaactt
    11161 catctatctc aaggcgctta atagcatctg gctgaggttt aggaagtttt attggattga
    11221 atccatcagt tatattatta tctatctgag agtttctgta taagaattta aatagagtag
    11281 agatggatga tttttttctt gctaaagctc tattgttatt ttcaaaaatc aatgtgtttt
    11341 tttctgtatt tttgtaatat ctactgcaat aatcacctaa gaataaattt atatctcttg
    11401 attttattgt attaaaatca tcttctgtaa tgtcttttac acaatctgca tttgttaatt
    11461 ctttggtttc aatcaaatat gaacaaaaaa aattaatatc ttctaaataa gcaagtcgtg
    11521 tagaaactgc aacagaacct tttaaatata taaaataatc cttcataaat acaggaagtt
    11581 tattttccaa aagtagacat ttttctatga gttcattttc tttgataaca atattatcag
    11641 gtttatctga tttattatgt acttttataa ttttcttatc attattcaaa attaaacctc
    11701 ctaaaactaa aatataaatc ttttatatta ttttttgata tttatactaa gtgagtataa
    11761 atatttaatt taatcatttt ttacattttt aagagattag cagtgcttta gatgaaatta
    11821 tcactaacta ttccctaaat ccacatttaa agactagttg aatttaagta ctc
//
LOCUS       DAMJYX010000101        11448 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJYX010000101 DAMJYX010000000
VERSION     DAMJYX010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.104"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(147..1433)
                     /locus_tag="PC479_002896"
     CDS             complement(147..1433)
                     /locus_tag="PC479_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2805424.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1556..4654)
                     /locus_tag="PC479_002897"
     CDS             complement(1556..4654)
                     /locus_tag="PC479_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2805425.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5380..5538
                     /locus_tag="PC479_002898"
     CDS             5380..5538
                     /locus_tag="PC479_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805426.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(5825..6529)
                     /locus_tag="PC479_002899"
     CDS             complement(5825..6529)
                     /locus_tag="PC479_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805427.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6520..8127)
                     /locus_tag="PC479_002900"
     CDS             complement(6520..8127)
                     /locus_tag="PC479_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2805428.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8381..9559)
                     /locus_tag="PC479_002901"
     CDS             complement(8381..9559)
                     /locus_tag="PC479_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805429.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9785..10279)
                     /locus_tag="PC479_002902"
     CDS             complement(9785..10279)
                     /locus_tag="PC479_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2805430.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10280..11041)
                     /locus_tag="PC479_002903"
     CDS             complement(10280..11041)
                     /locus_tag="PC479_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2805431.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         3813 a         2056 c         1148 g         4431 t
ORIGIN      
        1 tctagtattt tatattaata atttattcta gtattttaca taattatttg ttctaatttt
       61 tttcataata agctttaata ctttatatag taatttgttg tagaatttta tacaatataa
      121 tgatttatac agttttataa aatatcttaa aacagtattt cagtaatatt ttttggtctt
      181 cctccaccaa tactagtctc ttttgcacat attcgaccta aacctgcatt tactatttta
      241 tttaatatac gtcttgcact tctttcactt acatttagat actctgctaa ctcttttgaa
      301 tcataaatct tactttttct attctcattt atagcaataa tttttgcaac tgactcacaa
      361 ctcattccag tctttttaga tatttcgatt accttttcat cagatagcat caaagaataa
      421 tttaattcat tttcggaagc taaaggtcct cttatgtttt catcttcatc taccaaatat
      481 ataccaattt cttttgaatc tatggagtgt cttaatgctt tatgtgcatt attttcagcc
      541 tgatacgcag ttactccagt accaattcct atactaagag aaaagcccat gtcttttatc
      601 tctctcttta gattgaatag cttatcataa ttatactcat tatcagctac ccctttatga
      661 acaaatacaa cataatcatt tcttccaagt aaaaataagg aaccttgaat gcttcttaca
      721 tagtttacta caagcttatc catatctgat ttttttatca tgtcagaata atagttatct
      781 atcttctctt tataatctat taaactcaat atttcaacag caatttggga aaatcttgcc
      841 ttacttagag catattcact ctttaccttg tcataacttt ctttgattaa ttgtatagta
      901 gcttgtaaac gaaatacagg atagcctctt tcttttaatt cattatatac tgcaccaaaa
      961 ccagtcaaca ttatatccgt cttattttct tcaaataatt ttatatgcca ttctacaagt
     1021 tcagactctc ctacttccaa agaaaatgga ttacaaaata cactcgttgc tttaatatca
     1081 aattctttaa gtgcatcttc cacaacatat ctatcaacta catctatact gaatttatct
     1141 aattttatat ttgcactttt tattgcccaa aatgccttca ttatactcgt cccacttctc
     1201 ggtacaaatg aatgtggctt acttatctca tacttagatt ttataaattc ataaactgca
     1261 catcctgtaa atataatagc atcacattcg tcttcacatt tagaaataac ttctattgta
     1321 tctactactt tttctcttac atatatttta gtctttaaat cactgtctat ctgttttaaa
     1381 tcatccttta tcttaaaccc agagtctgaa ggacctataa tacccactat cataaacttc
     1441 gctcctaatc cataaatatt atacttttta aaaattatat cctcactatt ttctacttct
     1501 atttaattat tttgaactac tccatcctac ctacatttag aatggaataa ttagcttatt
     1561 tttgataaag taacttctca aaatcagcac ttggaatagg cttacaataa taataacctt
     1621 gaattaccac acaatctaaa gtcactaaaa attcagcttg ttcttttgtc tctacacctt
     1681 ctgccactac atccatatct aaatcttttg ccatggctat agtgtgcttt aaaactattt
     1741 tttctctgtc tgtgattcct tcctcattaa agaaaccaca atctatcttt aatgtatcaa
     1801 tattaatatt ttttaacata tttaatgaag aatatgctga accaaagtca tccatattta
     1861 aaacaatacc atccattcta aggtcggcta ttatcttagt aaataatttt atatttttaa
     1921 taaataaact ttctgtaagc tctaattcta aaaaagattt tggtatacta tatttattta
     1981 ttaaacttaa aacaatttct ttaaaatgtg gattatgcaa atgtaatctt gatacattta
     2041 cagagatagg aactggctct tcacctaaat ctatccattt tctaatcaaa gaacatactt
     2101 gttcccatac atacatatct aatctgataa taaacccatt cttctcaaat agtgaaataa
     2161 atttaattgg aggtattata cctttttctg gatgaaccca tctaaccaat gcttctgcac
     2221 caacaatttt atttgcatta agattatact taggttgtaa atatacatgg aattgcccat
     2281 tttcaagtgc gtattccatt tccctttcca tctgtttttc ttcaagtgca tcatttctta
     2341 tagtatcatc ataaaatata tagttattga tactttgttt ctttactttt ttgtgagcat
     2401 atcctgccat ttcacacgca tttgtaattg gaatatgatt atccaaaatt ctataaaccc
     2461 caaaacatgg tactaacata tattcaaatt tttttctatt taagctattt ttaatcttat
     2521 aaattacctt atgaatatct ctatcagagt aactctcata aaaaatgcaa aatacatctg
     2581 catacatacg acaacatata ccattagaac caatgttttt ttgaatcaac tctgcaacat
     2641 atattaaagc tttatctcct cctctaactc cataaatgtc attaattacc ttgaacttat
     2701 ctatatccat cattataatt gcataacgct tgtcaggata tcttttaagc attaaactta
     2761 tttgttcaca aaaactttca taattgtata gtcctgttaa tgtatctttt tggtttttat
     2821 gtagttgcat aagataatct atatttttta tagcaactaa aatatgtgtt tgtttctcac
     2881 ctaacttatt tttactacta atctttaaca tataaattaa tgcccaatag tactttttat
     2941 ctattcctaa acgtctatac tcaaaattta gttctaaatc gtcttgcttg ctattattaa
     3001 gactatcaag agattttaga aggtggttat cattaaataa tattatatca tcaggatgaa
     3061 ttaaatgttt ttttatgtat gaaaaagttt tttcaagatt ttcctttata ggaaaactaa
     3121 aaatattgct ctctgattga taaattggag taattaaatt cgtatcaatg caaatagata
     3181 aaattatatc atatgtatta taagctaatt tttcataagc cttagaatac ccttttatca
     3241 ttaaatcatc aaaataaacc ttatcaacta actctttatg tttaaataaa atattattcg
     3301 tcgaatcagc taagtcacta attactttca tttcattatt tctaagtttg ttcttattat
     3361 aatacctctt cttatcttgg tacattctct catcagcaac tttaattagc ttatgtatat
     3421 caatattatc gtcactccaa atactgccca ctgctacact atatatcatc tcatccttaa
     3481 attcttcact taaccaactc actaattctt caaatagttg ttttgatata tcttcacata
     3541 atacaacaaa ctcatctcct ccaatacggt aaacattata agaatgaaag tattttttta
     3601 ttattacaca tgctgacaca atagcagaat cgccattagc atgtccaaag ttatcattaa
     3661 tatcttttaa cccatttata tctacaaata caactcctat ggatttaact tttccttttt
     3721 gaaaattatc tatatactta atatatttat ttctattata tgcaccagtc aagaagtcat
     3781 agtagctcat ataatttaat ttattttcta cttttctttt tttcatctca gttaataaaa
     3841 aataagatag ggatgtcaaa agagaggtat ctccaacagc tttatgtgga ttatcaactc
     3901 caataaaccc tactacttta tcatcttgtt ttagtgaaga tgtcattaaa ctttcaattt
     3961 tttgaggttt taaaatttca tattctattg gctgttcttc ttttatattt tctatatcat
     4021 caattattac aaaacctttt tgttcgaata ataaaaacca attctttaat tcattaatat
     4081 caagatttgt tagtgcctgt ttttgttcat caacaccttc attgcaccat tcatatgtat
     4141 tagttacttt ttttctatct tctgatactt caaaaacata tgctctatca gctttatgaa
     4201 atttaccaat attggttaat acaagattta cagcatcaga aaaattagat tctgttataa
     4261 gatgtttaat acatccaact aataactcct ttatctctaa tttttctgct attgacttac
     4321 ttatatcttc cttttctgta atatcaagag ctatttctaa tcgtgcatgt ttgccctccc
     4381 atttaattaa cttgtccttt aatataaaat gtctatttgt tcgagtattc gtattttccc
     4441 atatgtaata ttcatcttct tttaaaatag agtttgtaca aaattcgcag ggagaatctt
     4501 tcccctgtaa tgctttataa cattttaaat gggatatatc attaagtccc attgcctttt
     4561 tcccaatttc attaatatat aaaagttcat atgtatctaa agaagaaaca tatacaattt
     4621 catccaactc atctattaaa tttattacat ccatttatat atatctcctt ttattcattt
     4681 tccagattga atttttaaag cttttaaaat tttgctatat atttggcaat gatattatat
     4741 ttctttatgt tttaaaatag taccttaatg aggttctatt ttttagtaaa tagaattatt
     4801 attgtgtcaa tcgtacaata gagttttgta atacctaatt taatatattt ataattaatt
     4861 atctcattat gtgtttataa ttaattctca tataaatgac aaatctccac ctcaattaag
     4921 tattccaatc atatatgaag ataatacttc attcatttaa aaatataaaa ctattatata
     4981 ttaaaatttc ttcaataata aattatctag attgttactt tgatatatta tataatatca
     5041 caaaggctga tataaaaaga caaaaagggc aattttttta tatcaaaaaa aaatgttaaa
     5101 ttgatattat ccatatataa attgatatta tccatataga tgctatattt aacaattcta
     5161 cttacttccc ttacacttat tagcatatag ttattaagag tttgttgcaa aacaacactt
     5221 agcacacttt aaaaagtgtg gtctaataag taatcaacga agaagacata ataaagcata
     5281 aaatatgata tacttttcca cgccatcaaa actgaggatt ttattattaa tatatgctaa
     5341 aatatgctta ataaattaaa ttttaatggg agaatagtta tgaaaatatt tagtttcttg
     5401 ttgtttgtga taggaatatg tttatgtatt gctggattta taggacatta cccagtattt
     5461 gttggtacaa cattgagttt tcttgcaatt gcaatgatag ctgtaagaca agtcaaaact
     5521 aagaaaaaaa tgaaataggc aaatcctaac tttacaagtt aatattacac aagtaattag
     5581 tctaaaattg gatacttttt ctattacaga aatacatcat ccactttgta ttttcttaaa
     5641 aaaataaaat agctatgtaa attcaagatt agatagatta actctctatc catttcattg
     5701 taaaatatac atagctattt gtattcgatt taaaatactt agattttctt acataacaaa
     5761 taatttctat ctgattatta ctcatattca tttagtaacc actcagcaac taatcattta
     5821 gtatctattc atccgccata cggtaaccta ccccaacctc agtcaaaata tattctggct
     5881 gagcaggatt cttttcaatt ttccttctta tacttgccat aaaaactcta agcgactgat
     5941 tttcactttc atagtaattt ccccaaactt tatctattat aaacttatgg gttaagactc
     6001 ttcctgaata cttagacatc aactcaataa tcttatattc gattggagtt aaatgtattt
     6061 cttcaccatt aattgacaca atatgatttt cataatctat ctttaggttt ttaacttcaa
     6121 aagactttgg agcatcatta tttatattat cctgttgagc tttatttcta agagccacac
     6181 gtactctagc caaaagctct gttacactaa aaggtttagt taaataatcg tctgctcctc
     6241 catctaaagc ttccacctta tccctctcat gctctctcgc tgaaacaaca attattttat
     6301 tagtctttgc acatgcccta actcgtccta ttacctcaat cccatcaata tctggtagtc
     6361 ctaaatccaa aattatcaaa tctgggtcaa gagaatgtat aagtgatata gctgttcctc
     6421 cattatctgt ctctacacac ctataatttt gagtttcaag agatactttt ataaatttac
     6481 aaattggctt atcatcttct acaattagaa ctaatggctt tatttccact tgtctttccc
     6541 ctcctcatca tacataggta tgctaaatct aaatgtagct cctccagaag gattattaaa
     6601 ggcgacaatt tctccaccat gagcttctac tatagattta catatctcca atccaagacc
     6661 aatacctctt ctttcaagat ttctagactt attcttagta taaaacctat taaaaatatt
     6721 cccgatatct tcatttggta ttccttttcc attatctgca acttcaaata gtacttgatt
     6781 tgactctttt tttacagtta gttctatctt agaattttta ggtgtatgcc ttatagcatt
     6841 atcaattaga tttactaaca cttgttctat taataataca tccacatgaa cgattattat
     6901 ctcatctgga atatttgttt ttatgtctct actattggca aactttttga ctcttaatat
     6961 agattcagct attatttcat caacaacttc tggtcgtttt tcaatatcca attttccttc
     7021 atctattctg gtcatactaa gtatattctc aacagaatga gttagccaac tcgtatcttc
     7081 atatatattt ttcaataatt ctactctagt ttctttatct atcacatcat cattttcaag
     7141 tattgttgaa gatgaaccta atatacttgt aagtggagtt cttaaatcat gagaaattga
     7201 cctaagcaag tttcctctta atctctctct ctctgcctca agatttgctt ttttattttt
     7261 ctcaaataaa ttttctctat caattgccaa cgcaacctgt gttgaaactg cttctaatag
     7321 tatcttttca ttttctgtaa taatatctat cttaaaacac gcaacaccta taacaccaag
     7381 aacatttgat tcttgacctt ttatagggaa ataatatgca ctgctgtttt tacaatattt
     7441 tgtcccaatt ccaactgggt tacctgtcct aaaagcttct tttatagctt ctttttctaa
     7501 tactgaatgg aatatattct cacttccatc attgttacaa atataaatac ttggcttacc
     7561 tagattatta ttctcatctg caatagtcac tattacagtt ctatttaata tatttactaa
     7621 attagaccca caaaattcaa taatttgatt tttatttttt gctttaagca acgttttgtt
     7681 attctcatag agcaatctta tcattttttc tctcttatga gaaagtctca cttctctttt
     7741 tatcctagaa gttaaagtac ttgttataat tgctgccatt agcataatac aaaaggtcat
     7801 aggataatct tttctataag ctaaaaaagt atataatgga tgcgtaaaga aaaaattaaa
     7861 tgatagcact cctattactg aagaaagtat tccaaaaaaa tatccatagg ttatacttgt
     7921 agcaaatagt acacctaata tgtatactag tataatatta gattcattaa aacctacctt
     7981 ttcaaaaact actgatattc cagtagcaat tgtcataacc aatatagttt ttatgaagtc
     8041 cttaataatt atctttttac tgagatagcc atcttcatca taatcatact cttttaagtc
     8101 ttcatgttgt agtcgttcct ttttcattta ctacaattcc tccttattgt tactgtttaa
     8161 attggctcta ttttatataa tacaatttta tcataataaa ttcaatcaat acatttaatt
     8221 aatgtactta aattactaaa aggtgctgtc ttaaaatgaa aataaatctc tatttcaaaa
     8281 cagcacctat atatttaaat tactaattta gtttttacat acttaaatca atagttttat
     8341 tcaatttcat aaattataat acttcaatta taaaacattt ctatccaaca agcataaata
     8401 taaagtgggc acatatacct gcacataaac cacctatagt atgcgaaagt atcgctttac
     8461 cagccaatgg tcttgcatct aaagcatcca tcatacctat gtgtgtactt aaatatccac
     8521 tccaacacat acccattgct gtgaatactg ctatatcatt aggagttata gcgcctgatt
     8581 ttataaactc tggtactaaa gatattgctg ctccaacagc tcctaatgca gttacaggga
     8641 atgctattgc ctctggtgat gtgaatccaa ataatggttc tattatgaag ctaatcttat
     8701 caccaatagc aggtaataat tttatacctt catatgcagc tcctgtgtaa acagcttgac
     8761 ctgtagcagg gtctgtagat ggtccaaaag ttaataacat aactaatgta caaactacta
     8821 aaactcctgg tattatagcc atacccattt caacacctaa cttaccacct tcaagtatag
     8881 catccaatgt tctttggaat acattaccat ctcttatttc tctaaactct tcaagttttg
     8941 cacctttatc atttatttgc ttttcagtat catttttagg gtcataatta taatactttt
     9001 tagtaaaagt caacataatt ctaacactaa taatactacc tattattgcc gcaacattac
     9061 ctattatagc tggtagtaca tattctttac cttgagctat cataaaagtt gttacaataa
     9121 gtcccatacc aaaggctgtt cctaaattac ataaagcagg tacttgatac ttcttaaaat
     9181 attgtgtaaa tgttttgtct ttagcaaatg gtataatagc tggattatca gataaatatg
     9241 ttgcaactac tcctgctata ctagcaccag gtaagcccca tataggtctc ataagaccct
     9301 taaatatctt attaactaaa gatattactc caaactctga aagcaatgca ctaagtgcac
     9361 ctgcaagaac tgccatagcc attattaaaa atactgtgtc taataaaagc gcatgtgctg
     9421 tactcattat tactttaaac atcattcctg cacccatgat gcttcctaca tatccaaatc
     9481 ctaccgccaa tagtacaata aatacgaagg tttccatact tactgccttg actcttctct
     9541 tatccgacag cttttccatc ttgaaatctt ccccctttta attttaaatt aaggttccta
     9601 acattggtac aaattttttg gtacaaactg agttctaaaa cttacactcg taatgcaagt
     9661 tgtcagtttt ttgaaataaa aaaaaattac gtttatagaa taagaatatc ctctttaatt
     9721 tggatactct tattctattt tatgttatta tattatcatc cttttgttta aaagtcaatt
     9781 ttttctattt ttgttgttta attttattta tactcttttt atatttttca ttttctttta
     9841 tctttgtaag tactccatat aagttgactt ttctaaatac agtagttata aataccaatc
     9901 ctaatgcaat tgaacttgca cacgataatg tactcacccc atattctaca gcttttatat
     9961 aattattggt caaaacagaa tacatagtat tatatattcc ataacctggt actaatatta
    10021 ttaaacagca aacacttaat gtagttgctg gagtcatata aattcttgca cataactcac
    10081 aatatatcgc aaaacaaact gtagctataa aaaaacataa gttttctgtt actcctatat
    10141 ttaaaagaaa tttatatgaa aaccaagcta tacttccacc tattcctgct agtattaagt
    10201 tttttccttt catattaaat agcactccaa agcttaaggc agtaaaaaaa gctgctatta
    10261 cttctataaa caaatgcact tatatccccc ctaacttgaa gtacaatcca agtaccaaga
    10321 acattcctac tgctatagat acacctacaa aaaatacttc tgctaatttt gcaagaccag
    10381 aaatcaactg tccatctaac aaatctctta atgcattagt caaagcaagc ccaggaacta
    10441 agagcattat tgctcctgct attaaatggt ctaacgtctt tatgaatccg agctttaaaa
    10501 ttaaaattga acaagaagct ataataactg ctccaattgc atttacaaaa aagtcattaa
    10561 gggaagattt acttaaatac ataataacaa acttagtcag aatcccaaca gtaaatgcag
    10621 ctataaattc ttccacattt cctccaaata atatcgaaaa agttgctgct gcaatacctg
    10681 caaaaaatat tgttattaaa tctgagtaat agttatcttc acttatttca tctaaaattt
    10741 tctcacataa ttcaatatct aaattattta tacttattgc tcttgataat gagttaactt
    10801 catgaaccaa gtttaggtct actcttcttg agcttattct tttaattgct gaatgtaatt
    10861 tcccatctac aaatacagat gccattacag ctgttggagt tgcaaataca tctacgtggt
    10921 ctattccaaa agattgacat attctagcta tcgtttcttc aactctatag gtttctcctc
    10981 cactttgcaa catagcttta cctgcatttg aggaaaagtt taaaattcta tctatatcca
    11041 caaaacatat ctcccattct tttatagtat tattcatatt ttaatacaag atattattta
    11101 tttaaactat tttaaataat tcatatccaa tatttacatt ttatgaaaaa acttgttttt
    11161 tatgcttgtc aaaatcaaat tttaagcttg tttacaccat aaaatggctg aattaatatt
    11221 tgacaaacac tttcaaccat ttatataacc ttctaaattg tataactttt tactacaaat
    11281 aaaattatat ctcttttaat tctttctctt gagtatttag cttatagtaa cttcatattt
    11341 atatatgtac aatataaaac attattttta tgatacaaaa tataatttgt aaatgtatat
    11401 atgtattaac tgtgtatata taaattattc ttgaccttat cagttttt
//
LOCUS       DAMJYX010000102        11365 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJYX010000102 DAMJYX010000000
VERSION     DAMJYX010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.105"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(60..626)
                     /locus_tag="PC479_002904"
     CDS             complement(60..626)
                     /locus_tag="PC479_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2805432.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            792..1313
                     /locus_tag="PC479_002905"
     CDS             792..1313
                     /locus_tag="PC479_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2805433.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2112..4190
                     /locus_tag="PC479_002906"
     CDS             2112..4190
                     /locus_tag="PC479_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2805434.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4190..4969
                     /locus_tag="PC479_002907"
     CDS             4190..4969
                     /locus_tag="PC479_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2805435.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4962..5771
                     /locus_tag="PC479_002908"
     CDS             4962..5771
                     /locus_tag="PC479_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2805436.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5791..6273
                     /locus_tag="PC479_002909"
     CDS             5791..6273
                     /locus_tag="PC479_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2805437.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6294..6698
                     /locus_tag="PC479_002910"
     CDS             6294..6698
                     /locus_tag="PC479_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2805438.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(6854..8014)
                     /locus_tag="PC479_002911"
     CDS             complement(6854..8014)
                     /locus_tag="PC479_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2805439.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8222..9772
                     /gene="xylB"
                     /locus_tag="PC479_002912"
     CDS             8222..9772
                     /gene="xylB"
                     /locus_tag="PC479_002912"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2805440.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            9868..11205
                     /gene="xylA"
                     /locus_tag="PC479_002913"
     CDS             9868..11205
                     /gene="xylA"
                     /locus_tag="PC479_002913"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2805441.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         4378 a         1308 c         2053 g         3626 t
ORIGIN      
        1 tataaaaatg agtatatagc attttaaaga gtaataaacc ttacataata aatgatttat
       61 taatatgaac tagaagtatt tttactatat tgttttataa aacctttagg cattttactt
      121 ataaaatata ccaccataat acaagacaaa ggtttgtcta ctatattact aagaatcctt
      181 ggaaagaacg cagcagtaaa tatcttctga ccagactgaa ctaaaaaacc aaccaataaa
      241 tcagcaccac ttccacttaa accaccaaac aataaaactg aaataggagt accaattaaa
      301 ggacaaacaa tagataaaat tactcctgta ataatagctt ctttataacc aaaacctttt
      361 ttcttagaaa tatatccaac aataagacca aggacaatat ttacaagggc aaaaggtata
      421 tttgtaggac ctgatataac acctaaaact aagtttgtac atccaccaat aagagcaccc
      481 caaaaaggac ctaatacgac agccccaagt acagtaccaa ctgtatctaa aaataataat
      541 ggtatattta tcatagatat aaccatacct aagacaatat taataactat gcacattgaa
      601 gaaaatgtga cttttttagt atccaattat aatacctcct aaaaaaatat atacatttat
      661 catagtatag attataaaag attactaatt gtatatttct atatagaata ttggaaatat
      721 agaacaaata aataatataa ttatcaaaat atgtagtatc attaaaatta agaataacta
      781 aggagattat tatgaaaaga aatagagaga ttgtagtagt atgtcattgt atattaaatt
      841 gtaattctaa agtggaggga ttgtcagaat ttaagggagc acaaaatata gcgataaatt
      901 taataaataa agggtatgga ataattcaac taccatgccc agaaatgatt atgtatggaa
      961 taaataggtg gggacatgta aaagaacagt ttgacaattt gttttttaga gaacaatgta
     1021 ggcaaatgtt aatacctcat gtaaggcagt ttgatgaata tttaaaaaat gattatgata
     1081 taaaaagtat cattgcaata gatggcagtc caagttgtgg ttataataag acatgtagtt
     1141 ctaaacgttg gtttggtgag ttaagtggtt gcaatgaatt gatggataaa ataaaggata
     1201 tacaaatggt ggatgggaaa ggtattttta tagaagagct agaaaagcta cttaaagaat
     1261 ataatttaga gatacctata gttggatttg acgagcttag agaagataag tagagatata
     1321 aatatttatt gtctcattat gaaacaccat tatatttggc agaaaaatat gactgatgga
     1381 cagtacagga accattttta agtggagtaa ttagtactta taaagatgta tccactagct
     1441 gaaagaaaat gtagaaaggt aataaatgaa ttaagaagta gataatatat ataaaggagt
     1501 gtctaaaagt gaacttttta gtttatgaga cactcttttt ttatattaaa tcaagtatat
     1561 taaatcaaat atattttaat cattttagca atgaaaaata aatttatcta tattttgaga
     1621 caacatcttt tttagtttta tttatttgtt gtcgtttttg acttggtata caattgtata
     1681 ttttcttaaa taatttcata aattatttat aattataaaa tccattgttt tgaacaatat
     1741 ctaatatatt attatctgta tttataaggt cactatacat attttcaagt ccgatattat
     1801 ctacataata ggcaaatgtt attcctgtat gtgttttaaa aaaactagat gtatataggt
     1861 atcatcacag atattaaaag tataaataaa agatattaag aacaactttc cagcaatata
     1921 ttagaaaatt ttactaaaat ccggaaagcg attgacaaac aaagtcaaat aaagtatatt
     1981 attggtaagt tagtttaatg tacaaatgaa ttatatataa aacctaattt ataaataacg
     2041 atactaaacg acattattta acttaagtta cacaaagata gaatattata ccattgattt
     2101 aggaggtagc cgtgataaaa atatctgaag actatattga aaaaagattt gacaatgaac
     2161 tattgcgaat agaagcatgg ggaaaaaact ctttacgtat taggtcgttt gtagaccaaa
     2221 attttgttga tgaaaactat gctcttaacg aaaaacctaa gttaaataaa gatgatatta
     2281 caattaataa aaatgaagat ggaagtgcaa tcattaaaaa tggaaagata aaagctgtct
     2341 tagaccatag agatagaatt actttttata atgataaaaa tgaaatatta ttgaaagaat
     2401 atattcgatt aagagctgta aaacatgatg atggtggaga agatgtaggg acaattgaga
     2461 ttaccaaaga ctttaactct actctaaagt taaaatcaag agaatatcga ccaagctata
     2521 gtggagaatt tgaagtaact actagatttg aatctgagcc ttctgaaaag atttttggaa
     2581 tgggacaata tcaacataaa tttttagatt taaagaatac tgttttggag ttagcccaaa
     2641 gaaattctca aatatcagtt cctttttata tttctagtct aggttatgga cttttatgga
     2701 ataatccggg aattggaaaa gtatcttttg ctaaaaatat gacagaatgg aagatgtttt
     2761 ctacaaattt tatagattat tggattactt gtggagaaag cccaaaagag ttaaataaaa
     2821 attactctca agttactgga actgttccta tgatgcctga aaacctttta ggactatggc
     2881 aaagtaaact aagatataga acttcagaag aagttttaga tgttgtgaag gaatattcta
     2941 aaagaggaat taagctttca tcaattgcaa ttgactattt tcattggcct aagcaaggtg
     3001 aatataaatt tgacttagat tattggaaaa atcctaaaga attagttaaa aaattgaaag
     3061 aagagtattc agtagaacca atagtatctg tatggcctac agttcaaagt gatgcagaaa
     3121 actacaatga ttatttagaa aatggctatt tagtcaatgt taatcgtggt gttagaatga
     3181 caatgcaaat tcaaggaaat acagtctttg tagatatgac aaatgaaaat gcaagagaat
     3241 atgtttggga tagaatagat aaaaattata aacaacttgg gattgactat tattggttag
     3301 atgtagctga acctggctat tctgtgtatg attttgataa ctatagatat aaaaaaggaa
     3361 atgttttaag ttgtggtaat atttatccta ttgattattt aaaaatgatt tatgatggtt
     3421 tacataatga catagagtct gttgtaactc ttgtaagagg agcttgggca ggagctcaaa
     3481 agtatggagc tttagtatgg tctggtgaca ttgattctag ttttgaagct tttaataatc
     3541 aagtaaatac agggttaaat atgggactgg caggtatacc atggtggact actgatattg
     3601 gaggtttcca tggaggaaat cctaaagacc cagaatttag agaacttatg gtaagatggt
     3661 tccaatatgc tactttttca ccaattttga gaatgcatgg agatagattg cctcatagta
     3721 aaccattatc aaataaaggt ggaggctcaa tggtaactgg agctcctaat gaaatatggt
     3781 cttatggtga agaagttgaa gtaattctta caaaattcat aaaaattaga gagagcttaa
     3841 aaacttattt aaaaaaactg atgaaagaag cgcatgaaga tggaacacca gttatgagaa
     3901 ctttatttta tgaatttcct gaagatgata aaacttggga ggtggataat acatatatgc
     3961 ttggagatga aattttagta gctccaataa tgaattataa agatagaagt agaaaagtat
     4021 atttaccaaa aggacataca tgggaaaata tatttagtgg agttagttat gaaggtggga
     4081 aaacttatga agttgaatgc ccactggaag aaattcctat tttcctaaag caagattcca
     4141 gttataattt taaagaacta aaaaatattt tgggagaggt gaaatcataa tggagccaca
     4201 attatggcaa ttattattaa tcgtactata tggatttttt ataaactatg aaaaaaattc
     4261 aacaatgttt ggaacatatc aaccagtaac agcaggtttt attactggtc ttatcttagg
     4321 agatataaat acagggttat atattggagg tacattacag ctattatcat taggtattag
     4381 taactttggt ggagcatcaa tacctgatta tcaaacagca agtatagtag ccacatttat
     4441 aactattacg actaagcaag aagcatctgt aggaatctct ataggtattc cagttgcttt
     4501 attgatggta caattagatg ttcttagaaa tactattggt atatggctag ttcataaagc
     4561 agaagatgga gctaaaaaag gaaattataa aaatataacc tatatgcaaa tgttaggagt
     4621 actattaaca gcagctacaa caggtattcc agttgctcta tctgtaatat ttggaccttc
     4681 tttaattaat accattttga aatatacacc tgaatggtta acaggtggtt taacagtagc
     4741 tggaggacta cttccagcag tagggatagg attattactt agatatcttc ctgctaagga
     4801 gtattttagt tacttagtaa ttggttttgt tttagcagta tatatgaaag taccattatt
     4861 aggtgtagct ttaattggtg gagcaattgc acttataatt tacaaaaaga atttggaaaa
     4921 tcaagaacaa caatatactg tagtaggagg tatggacgaa gatgagtgat aaggtatcat
     4981 taaataaatc aaatgaaaag gtgaaaaaaa gtgtcttatg gcgctggttt tttactagtt
     5041 ctgtttcttc aaattatgag aagatgcagg ccttggcata ctgttatgca gtactacctt
     5101 ttttaaaagt agtatacaaa gataatccag aaaagttaca aagtgctgta ttaaatcatt
     5161 tacaattttt taacactaat ccttgggttg ccccttatat tttgggtatt aatgtagcaa
     5221 tggaggagtt atcaagtgaa gagactgaag aggctgttgc ttcaattaaa acaggcttga
     5281 tgggaccagt agcaggttta ggagatagtt tatttgtcgt tattccgtgg acaatttttg
     5341 gagctatagc agctaatatg gcgatagatg gaagtccatt tggaatctta ctgtggattg
     5401 tagtgagtgt agcattgaaa ttattaagta taccactatt taatgctggt tatacatcag
     5461 gtactaagct aatatcttca attgaaaaaa gtttaaagat tttaactgag agtacatcta
     5521 ttttaggttt aatggttgta ggtgcattaa taccttcagt tgtaaaagct aatgttgctt
     5581 tgaactttca acaaggtgat tttacaatga aaggtcaaga aatattagat caaattatgc
     5641 ctgggttagt tcctgcaatt ttagttgtga ttgtttattg ggctcttaaa aagaatataa
     5701 aacctatata cttaatttta ggagtaatgg ttgtttcaat aattttatat gcattaggta
     5761 ttttaaaata aggaaaggat gatattagat atggaaatga aaggtattaa aaatattcgt
     5821 atagatgacc gtttaatcca tggtcaagtt gcaactatgt ggagtaataa attgggtgtt
     5881 acaagattaa tggtagtaaa tgatgcagta gctaacaatt ctgttcaaaa acaagtttta
     5941 agaatggcta cacctgcagg tatagcgtca tctataatta cagaagagac tgctataaaa
     6001 aatattactg caggaaagta tgaaggacaa aatgtattat taattgttaa gtctcctgtg
     6061 gaccttattc catttgtaga ggcaggattt aaaatacata atataaatgt aggaaatatg
     6121 tcaagtagaa aagatacaac agttctaaga cctaatatca gtgttactga agaagagaaa
     6181 gaagcattta aaagactttt agatgaagga attgaaatta caactataat gacgcctgat
     6241 gataagaaaa cttatcttgt agacatctta tagatttaaa gaggtgtatt aaattgaata
     6301 gtattatttt gatgagtcat ggcaatatgg ctgaagaagt cttaaattct gcaaaaatga
     6361 taatagggga ttctatagat tatcctactg taaatatgaa acaagatgat ggaatagaag
     6421 gaacaataga aaaattaaaa aaagctttag aagtgtacaa ggaaagttca gaaataatta
     6481 taatggcaga tttactaggt ggaactccat gcaatgctgc tttattagta gccaataatg
     6541 acagtcgaat taaaattatt tcagggctaa atttaggtat ggttattgaa tcagcttttt
     6601 tagatacaaa ggaattggca gcaaaattag agaaaatagg taaagataat atttctatgg
     6661 ttgaacaaaa tgcaaattta ttatctgatg atgagtagga agattatatt aatatttaaa
     6721 acgaaatact tatgttaata cttaattgat atacaatttt taaaaaatat agctaatctt
     6781 gcataaacta tataataaat ctaattttag aatgtataat ttaaagggtt atggctgtat
     6841 atagtcataa cccttatatt tcattaatta aagttaaatt ttgtatattc aaaaagctct
     6901 tgatatttac agcagagcca ccatatagag atgcctcaga ccctaatgat gaaatttcta
     6961 tatttattcc tttgttaaaa gagctaacta acatatcttt tgtaatctgt aatatctcag
     7021 ggatatcact aattatttgg ctatttaaat atattatttc aggagcataa gttgtaatag
     7081 cattatttat agctattgtt aagtaactac aaaactcatg aataactttt ttggcattat
     7141 ggttatcttc ataatatagt tgctttacta tatcagaatc tattttagga atattttcta
     7201 aagaagaaag ctgttcaaat accttttttt ctgaacaata ctgttctaaa cagccacgat
     7261 ttccacaagg acatagttta ccgttaggca tgatgatagt gtgaccaatt tctccactca
     7321 taccatttct gccactatat aatttattat ttattataat gcctgaacca aatccactgt
     7381 gaatactgag actaagtagt gagttatgta cagttgaaaa agtattctca gctaaagctg
     7441 ttaagtttgc ttcattttct atatgaatag gaaagtcata ctttttgcta aggatgctat
     7501 ataagtcaat ctcattaaga ttgtaatatg gagtaaacaa tactttattt tcacaagtaa
     7561 tcccatgtat agccaaagta agaccaatta ccttataagg agtgtctatt ttggatatgt
     7621 tataactatt tataatttca tctatcaatt gtataacatt gtctttactt acttgtatat
     7681 ctgttaattt cttagaattt ataatagttc catctaagta agatagagaa gaaaatatat
     7741 agtcgtatcc aatgtccata cttaaagaaa tccctgcaca tttattaaat actaataata
     7801 tgggttttct tccgccacta tgagtactat ttccaattcc tatttcatga actagagatt
     7861 catcaataag ttttttggta atagcggata tagttgcttt attcaatcct atagtagaag
     7921 ctatacttgc cctagaaata ggaccatttt ttataatttg ttcaagtacc aatctttcat
     7981 tcatttctcg aatagtgtat ttatcagtaa ccaatttgat attcctcctt aaaataatat
     8041 tgtaatactt tttacacaaa aataaaaggt tattttgcat tgacaaagat aattaaatat
     8101 tttattatta gttcataagt tagtttaata tactaacaaa aataaagcaa gtaaaatata
     8161 cctaaaatat aaaaaaatta ggataggaaa acgatagtta tgaagtggca ttcaaggagg
     8221 gatgaagttg gagtatgtat taggtgtgga tattggtaca tcaggtacga aaacagtttt
     8281 atttgataaa ctaggaaata ctataaaaag ttgtacatat gaatatcctc ttatacaaga
     8341 gaaatcaggt tgggcagagc aggatgcaaa tgattggtgg aaagctgtag tcgaatctat
     8401 aagagaagta gttcaaagtt caaatataag cagtgaatgt ataaaaggaa ttggattatc
     8461 agggcagatg catggtctag ttatgctgga caatgaaggt aaaacattga gaaactctat
     8521 aatatggtgt gaccaacgaa cagtaaaaga gtgtgaggag ataactgatt tagtaggtga
     8581 agaaagactg attgaaataa ctgctaatcc agcacttaca ggatttacag catcaaagat
     8641 attatgggtt agaaacaatg aaccagatat atataaaaat actaataaaa tacttcttcc
     8701 aaaagattat ataagataca aattgacagg tgaatatgct acagaggtat cagatgcaag
     8761 tggtatgcag ttgctcgata taagaaaaag agattggagc gatgaagtac tagaaaagtt
     8821 aaatatagat aaaaatcttt taggtaaagt ttatgaatca caagagatta caggttatgt
     8881 gactagagat gtatcaagtt tgacagggct taaagaagga acaatagtgg taggaggagc
     8941 tggagaccaa gcagcaggag ctataggaaa tggaatagtt aaagatggag tagtttcatc
     9001 tactataggt acatcaggtg tagtatttgc ttatacaaaa gagccaaaaa ttgataaaga
     9061 aggtagagtt catactttct gtcatgcaat accaaataca tggcatgtta tgggagttac
     9121 tcaaggagct ggcttgtcat taaaatggtt taaagataat ttctgtcaaa gtgaagtgga
     9181 agtttcaaat tctttaggtg aagatgtata tgagattata aataatcaag taagtaaagt
     9241 accaacagga tgtaatggat tgctttactt accatatatg atgggagaaa gaactcctca
     9301 tcttgaccct tatgcaagag gtgtgttctt tggattatct ccaatccatt ctaaaaaaga
     9361 aattgcaagg tcaataatgg aaggtgtttc atatagtctt aaagactgta tggatataat
     9421 agagaatctt aatatagacg ttaatgaagt aagagcctca ggtggaggtg gaaaaagtaa
     9481 agtatggaga cagatgcaag cagatatgtt taatcaagat gtatatacta taaattcaag
     9541 tgaaggacca gcacttggag ttgcaattct tgcattagta ggagctggaa tatatgaaaa
     9601 tatacaaaaa gcttgtgatg ctataataaa aacttcaact aaattagaac caataagtga
     9661 aaatgtagat acatacaata tatatcatag attgtataaa agaatataca aatctttgaa
     9721 agatgacttt aagttattag atgaagtagt taatgcaaaa ataaataatt aaattagctt
     9781 aaaaagaaaa ataaagatag aaaatagttt taaattaaga aaacatacta catatattat
     9841 atgtgtaaaa tattaggagg acatgaaatg agtgaaatat ttaaaggaat aggacagata
     9901 aaatttgaag gagtaaaatc tgacaatgaa ttagcattta gatactacaa tcctgaacaa
     9961 gtggtaggta ataagactat gaaagaacac ttaagatttg caatgagcta ttggcatact
    10021 ttatgtggag agggtaatga cccatttgga gtaggcacag tagagagacc ttggaataat
    10081 ataacagacc caattgagat agcaaagatt aaagttgatg caggatttga atttatgtct
    10141 aagatgggaa ttgagtattt ctgttttcat gacagagata tagcaccaga aggaagagat
    10201 ttagaagaga caaataagat attagatgaa atagttgagt atataaaagt taatatggaa
    10261 aagacaggta taaagttatt atggggaact gccaatatgt ttggaaatcc aagatttgtt
    10321 catggtgcat ctacaacatg taatgcagat gtatatgcat atgcagcagc tcaggttaaa
    10381 aaagctatgg aaataactaa gtacttagga ggagaaaatt ttgtattctg gggtggaaga
    10441 gaaggttatg aaactttact taacactaat acagaacttg aaatggataa ctttgcaaga
    10501 ttcttacaaa tggctgtaga ctatgctaag gaaataggat tcactggaca attcttaata
    10561 gagcctaagc caaaagagcc tactaaacat caatacgatt ttgatactgc tactgtttta
    10621 ggatttttaa gaaagtataa tctggataaa tacttcaaaa tgaatataga agcaaaccat
    10681 gcaacacttg caggacatac tttccaacat gaattaaata tagcaagaat aaacaatgtt
    10741 ttaggtagta tagatgcaaa tcaaggagac ctattattag gttgggatac agaccaattt
    10801 ccaacaaata tatatgatgc aacacttgct atgtatgaag tgttaaagca aggtggaata
    10861 gcaccaggag ggtttaattt tgactctaaa gtaagaagag catcttttga ggtagaagac
    10921 ttattcctag cttatattgc aggaatggat acatttgcaa agggattatt aatagctcat
    10981 aaattattag aagatgaggt atttgaaaac tttactaaag aaagatatgc aagttttagt
    11041 gaaggtatag gtaaggatat agtagaaggt aaggttggat ttaaagagtt agaatcttat
    11101 gcactgcaaa tgccagttat aaagaataag tctggaagac aagaaatgct agaagctata
    11161 cttaacagat atatatatga agtagataca atttctaata agtaattgtg tagtaattaa
    11221 acttggatgt ttgaataaaa taacaggata tgttcttgtt tttattctaa aacggacaaa
    11281 gataagtatt tgtaaaaatt ctcataatca tgataaatta aggatataga cttaagtata
    11341 attagtttat atccttaaaa tttct
//
LOCUS       DAMJYX010000103        11305 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJYX010000103 DAMJYX010000000
VERSION     DAMJYX010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.106"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(192..824)
                     /locus_tag="PC479_002914"
     CDS             complement(192..824)
                     /locus_tag="PC479_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2805442.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(896..1249)
                     /locus_tag="PC479_002915"
     CDS             complement(896..1249)
                     /locus_tag="PC479_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2805443.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1327..1530)
                     /locus_tag="PC479_002916"
     CDS             complement(1327..1530)
                     /locus_tag="PC479_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2805444.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            complement(1920..2150)
                     /locus_tag="PC479_002917"
     CDS             complement(1920..2150)
                     /locus_tag="PC479_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2805445.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(2152..2559)
                     /locus_tag="PC479_002918"
     CDS             complement(2152..2559)
                     /locus_tag="PC479_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2805446.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2919..3368)
                     /locus_tag="PC479_002919"
                     /pseudo
     CDS             complement(2919..3368)
                     /locus_tag="PC479_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3382..4308)
                     /locus_tag="PC479_002920"
     CDS             complement(3382..4308)
                     /locus_tag="PC479_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805447.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4433..5215)
                     /locus_tag="PC479_002921"
     CDS             complement(4433..5215)
                     /locus_tag="PC479_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2805448.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5371..5991)
                     /locus_tag="PC479_002922"
     CDS             complement(5371..5991)
                     /locus_tag="PC479_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2805449.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(6079..6777)
                     /locus_tag="PC479_002923"
     CDS             complement(6079..6777)
                     /locus_tag="PC479_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2805450.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6878..7693
                     /locus_tag="PC479_002924"
                     /pseudo
     CDS             6878..7693
                     /locus_tag="PC479_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8365..9132
                     /locus_tag="PC479_002925"
     CDS             8365..9132
                     /locus_tag="PC479_002925"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2805451.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9460..10137)
                     /locus_tag="PC479_002926"
     CDS             complement(9460..10137)
                     /locus_tag="PC479_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805452.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10287..10604)
                     /locus_tag="PC479_002927"
     CDS             complement(10287..10604)
                     /locus_tag="PC479_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805453.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10626..10964)
                     /locus_tag="PC479_002928"
     CDS             complement(10626..10964)
                     /locus_tag="PC479_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805454.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3782 a         1755 c         1588 g         4180 t
ORIGIN      
        1 ttttacctat tataataaaa acaggaattt aaataataaa aagtaaagta actttttatt
       61 aagtaacttt actttttatt ttatttaatg ttgtattaaa tttatatata ttaaaataat
      121 atacaaattt tgaaaaagct cttataaaat gattattaaa gtaaagttta atataaataa
      181 attataaaat attaaactaa tttatcaata tttcctgatt gaatatgctt aatgtttgat
      241 tgaatttttt tatatggcca atcccaccat ttaatttcta ataattttga aattgtatca
      301 ttattaaatc tcttttttat aatttttgca ggtactcctc caacaataga atacggtgga
      361 acgtcattag taactactgc tcttgttcca ataattgcac catcaccaat atttacacct
      421 gacataatta tagaatcata acctatccac acatcatttc caattataat gttaccctta
      481 ttgtcccaag cgtccgttat atggttgata tcgagtttcc attcttcata aaagatggga
      541 aaagtatatg tggattttga tttcattgag tgatttccac tagtcattag aaattttgca
      601 ttacatgcta ttgagcaaaa cttaccaatt attaatttat cattgtttat agggtaatga
      661 tacaatacat tgtttttctc aaaatctctt gggtcattat aaaagtcatt atatattgtg
      721 taatctccaa cttctatatt ttcccttgtt attacatttt taagatatac agtttgataa
      781 tcattacttc ttggatatac cttatttgaa cttggaatag tcatatttaa atctctcctt
      841 taatatatat tatgtttttg actattccac tactacaata catctcttca cagaatcact
      901 ccttgttaag aatcttcata agataatggc gtcctttttt agtgttaagt cggatattct
      961 ttcttagtcc attcttttta gcgtataact gacaaactgt cattttctta ccaatagtgt
     1021 caatgctatc atcaaggtca ttttgtattg ctttttcctt atcccttaat gatatatcat
     1081 cacgttttcc ctttgggggt ttatcagtag gtacaagttt ccaagagtaa acaaattgtg
     1141 gtttaccaaa tgaatataca tacttatatg cgtatcttcc atcttttctc tggctctctc
     1201 cagttcttaa aattctatct ttattatctc gtcttttttc agttgacatg ataagaagct
     1261 cctctcaata accgaattga gcttgatacg tcatatgaat attataacat ataaggctca
     1321 atttcattag ataacgtcaa gtgtatcaac gattttctca aattgttttc tctttatttg
     1381 aatgcgattg ctattcataa tgacccagcc agaatcaagg ttttcatcag cacgtttacg
     1441 taattttttc tctccaatac gaaagtattt tgacgcttct tcaatcgtca aggtatattt
     1501 ttcccaaata ggtacatcag tattgttcat ataatgtatc actcctttct atgttcgtag
     1561 ttcagaagta gatagatggc tttgataagc tccattggta tttcaaccaa tcccattctt
     1621 atggacagaa ccgtgtcata caggtgtatc attatactgt ggtacaggtt acggcaaaac
     1681 aaccgttcca aaggttattc acaaatcact atatagtagc tctcttgatt aaggtcatgg
     1741 cgtatagtat ttgttggcta tctgtatcac aaacgtatct atctacttta ttcagttgtc
     1801 aaagaacagt cggcattgat ttgcctataa aaaatcacaa tagcttgtgg gcttttatag
     1861 acaaataatt agtgattctc catgtaccaa atagcttgat acatggagag agggcgagat
     1921 taagacaaat taaatctcaa aatagcagtg attagttttg ttcctagcat tcgctttgtt
     1981 tcttcttcta catgaatata gggatttccg tattcatcaa ataaagtttt ctgtgaaaga
     2041 cgaatgatat agtgttcaaa atgtttaagt attacgttta ttgcatttac atcgccatgt
     2101 acagcaagta aaataattgt aaaaggtaat acttctttag taggtttcat ttcaatgttc
     2161 ctgcatgagt tctttcaact cttttagtgc atttgttttg tgataatgta tggtattttc
     2221 tgctaaatcc atcaatgttg ctatgtcagc atttgtcatg ttgaaaaaga atgaaagtag
     2281 aattatatct tgttttcttt ttgtaagttt tccgattgct tgcgccatta atacatcttc
     2341 aattcgtatg ttgtagtcgt aagaaaccat acagatgtag ttagaatcat aatcatctaa
     2401 aatactcaat ttacttaatt ctgcaagtga caaagattcg agagatacca aataatcatt
     2461 tctgcgttta ttttcgtcat aaatatctct agcatagtta cgcaaaactg ttttacagaa
     2521 actgtcgaat tgatgttcaa ttgtcttttc atcaaccata agatttcacc ctctttccat
     2581 cgcaaggagt taaataacct tgcttactcc tatcacggtg ggagggcgtt aaatcttcga
     2641 taaatttcaa aaaaaagaga aaaaaataca cactctgcct atagggcaaa aatgtgtatt
     2701 ctttaaaacg tatttaattt atattggttg taagccacca ccatattgat gtatggaaat
     2761 atgcattggc tagcttttca taaaaataca aagatgcata agaaattaat agaggggaaa
     2821 aacttattct gttattaaaa agtaggttaa ttctaataaa tgctattgcg gtcgaattaa
     2881 actatataca tggcaatcaa tcatgtttct attctttatt atgaattgtg acagtattct
     2941 tcaatcttaa agccacattt ttccaatact ttacttgact ctctattgta ggaatataca
     3001 ttagcatgaa ttttgttaag gttgtatttt tcaaaggcaa ttttgcatat ttcttttact
     3061 gcatttgttg ctatcccttt gcctcaatag ttcctagcaa gccagtaacc taattcagca
     3121 tttttattaa tagaggtatc ttcttcaaat gttaggctga tacttccaat agccctttct
     3181 tgataaaaaa tagcatagct aagttgttta acatctgaag ttgccaaaca aaaactaaca
     3241 aaatctctag cgttttgctc cctataaggg tgggggaagt tgtcacgaag attctttgca
     3301 atttctttat cattcgcaaa ctcaactaaa tcggtaatat aagcataatt ccactctata
     3361 agttccattt ttcttctcct tttatgaaac agctacaaga aatctttcta agccaatacc
     3421 acttatcaaa cagcgttctc ttttatttcc aagcatttga taagtccagt caacaaatcc
     3481 accgtcaact atttcaacgg tttcatttcc ttgtttgacc gataatttga aattgatacc
     3541 tttataataa ttattatcaa catcatcatt atgaattgat aactctatat taggaaaaat
     3601 aagtttgata gtttcaacca ttctgtcgaa aaatccgtca ttatctttgt atccgtttct
     3661 tttgcgtaga ataatagaga aacgaacatt ttctatttca cttaatatat ctttataata
     3721 gtttaagtgc ttttctaaca acattttttc acaagtgtaa gaccctgtat ctataccaga
     3781 tgaaaccata caatataagc caaaatgtga atataaacct ttacctacaa aattttgtgc
     3841 tcttattacc ctagcagtag tagccatgtg gataggtatg gtattatttt cttcttttct
     3901 ttttaattta tcagcaataa ttattgccaa catatttgag gggtctgcaa gtgtttctgt
     3961 ccctcgtaga gcacttacta cgttatattg atctacggag ccaaaaacgc tacagctacc
     4021 taatggggca gagggagaaa gcataatcgt cttgatatcc atattgaatg cttttctcaa
     4081 taattttgat tccaaagcat gaatttcttc tggatctata ccgctaggga tagtaaatct
     4141 atttagttgg tatgacttta gaatatcagt tgcagttaaa gtatttgctt gtctatcaaa
     4201 cacttctaat agaagtgagt tcaaatctga ttttgataga gctgataact tatcaactat
     4261 attcttatcc ccaattttat ttgtaattct acttaaaata tcactcatag ttatcctcca
     4321 tactgttact ttcaaaataa ttatagaatt gttacaacaa ctaatctagg tcatttgacc
     4381 aaaaatgaaa aaacactgaa tatttactat tcaatatttt tttactttgg gtttatttga
     4441 ttaaatcttg aaaatactgt aatgcaaccc tttcagcttc aacctttcgg acaataggac
     4501 tttgtgataa ttcctctctt aactcaagat acaggttgat agtagcctgc aatgcattaa
     4561 cacagctttt atattcatgg ttagcaaccg tcttttctaa tgcttcaata tattggggtg
     4621 cgtccaattc aatttttcta actccacgag gtaatttacc taagagaagc tgtattaatg
     4681 gggcaagtac tgattgtctt aaaaaagata aaaaatcaat tgtttcaaat agttctctac
     4741 gtccaatctt agtgcaagcg taatgaaccc aaatccaaaa tctatcttca atccactgta
     4801 agtctggagt tgggaagtat gcttcttttt tagaataaat ttctgttatg cagttgtttt
     4861 cttcatagag aatagctgaa tcttcaaccc tatctagtaa accatctaag gttgtaaact
     4921 taaaatcaac atgaagaaca ggggaatcat agagggaaat tatcaatcga ggttctccaa
     4981 cgtgttcgcc agtaaaagca gaaactaatg ttccaaactg ttccacaata agtaatctct
     5041 cttcgataac ttcttcatag gatttatcat ctgctacaat cagaaaatcc aaatcagaat
     5101 attcatctaa cgattttgtg atataagaac tgctaattgc aagtcctatt atcctactat
     5161 ctctttttga aaattctaat acgttttgta tcattttctg ttgtgtttta gtcataatat
     5221 aacacctctc actgtctttt attagctatc aatccaacat aattatgcat aggagtatta
     5281 tataatgaag aaataaaaaa aaataggtca cttgacccga aataaaaaag cattgaataa
     5341 ttattattca atactttttt gaaattatac ttatgttgta ggaagattct ttaatacctc
     5401 tcgctgatta attacactaa atcttttgtt ttcataagca attagatgtt cttgtacaca
     5461 ttcatttata gcacgattaa tgcttctaat tggagcgttt acttcgctag caagctgttt
     5521 tttggtaagc agatttaatt cacttctata atagtgtaag gatatacacc tcagaagtcg
     5581 ttctttaaca cttaaagatg ataattcttc aattaattca gcatttacaa ctaatttact
     5641 agatatttct cgaatcagaa attttgtagc ttcaaaatct ccccttaacc agtttaaaaa
     5701 atcatctcta tgaagtttat atacattaca atctgtgaaa gcgacaattt caacgggttt
     5761 tttcccatca ttaaattgtt ccatttctcc aaaaaaacta ccacttctat aaagatgaat
     5821 cgttgagatg tttcctttca catttaagat gaatgcttct gcaataccgt caatcataag
     5881 aattacatat tcattttctt gctcagcaaa aagaatagtc tctttctttt tatacgtttt
     5941 tttaacaagc ttctttttaa tatcttcatt cataaaatca attaaatgca tattcatccc
     6001 tccttaccaa atctttgatt aaattatatc atctttcaat tagttaggta agatagtaaa
     6061 aatatattta catagaaact attccattat tattttccag ttgtcatttt tctgcaaaaa
     6121 cttatcactg tcacgactgg agataccagt aaggaatgtt aggatatcaa tcgctaaact
     6181 ttcagaatcc tttttacggc tcaaaatgac gtaagggtca agcaatcctt tgttttcatc
     6241 attactggtt aaattgacaa tgtttatctc atgggctatt tctggtagcg tttctttcca
     6301 tttgccgcgt tctgctttag ggtcaactat gaccgcctgt ccgccgaata gtaccgcata
     6361 gtaaacaagc atattgttac tgaataattt accaccaccc aatgaaccga taaacgcaga
     6421 cgctaacgca ttggtaatag aaccctttac cccttgacta gcaagggcag gttttagata
     6481 gacattcttt ccagtgtcca gattgtagcc aagatagata tcctcatgtt cgccaagggt
     6541 ttgtgtcgct ccaaaaccaa gtccagccaa aaaatccgaa gtgacatatt gaatataatc
     6601 attgatgtaa cgtttgctga caggaataaa ttcgccatgc aatcccaaca tatccccaaa
     6661 gggacgcacg agcttgattg ataagtcgtc gtaaaagtct ttgacttcat cacaacgcct
     6721 ttttagctcg tcaagattct ttgtagatac acggacaaca tagctaagtt tatacataga
     6781 ttctttacag ttaaaagtct gtttttaaaa aaatgtataa attataaatc aaaatataga
     6841 tactatcaat gacccactag gtgcaaaaag cattgatatg gttaaattat cagacgctaa
     6901 aagctgggct atccgaatga gtgaagatgg gtgtgcttat aaatccattg acaattataa
     6961 gcgctcactg aaagcgtcgt ttttcatggc aattcaagac gactgtatta gaaagaatcc
     7021 atttaatttt tagttaagtg atgttttgga agatacaaca gaaccaaaaa tcgctctaac
     7081 accagaacag gaaatgggct tattatcctt tatggggagc gataagactt attgtaaata
     7141 ttatgatgat gttgttatcc tcttagaaac aggactacgc atttctgaat tatgcggttt
     7201 gactttagaa ttggatatga aaaataaagc cattatggta gaccatcaat tgttgagtga
     7261 tactaagatt ggctattatg tagaagttcc aaagaccaca cagggtaagc gagaaattcc
     7321 aatgactgaa agagcttatc aatcatttga acgtatcgtg gaacggcgta agaacgctaa
     7381 agcagaacca atcattgttg atggttacca gaacttccta tttcttaaac aagatggatt
     7441 accaaaacaa gcaactcatt atcaaagcat gttaagagcg ttagtcaaga agtataataa
     7501 gacccatgag gacaagctac caaagattac gccacatacc tttaggcata cactctgtac
     7561 aaatatggct aacaaaggaa tgagtcctaa tatattgcaa tatattatgg ggcataaaaa
     7621 cattactatg acgcttggct attatgcaca tggttcattt agttcttcga gagctgaaat
     7681 ggaacggtta taatcctagt agtatgacta gagtttacta cttatttact acttttgatg
     7741 gcattcttat acggttaaat gccaacttat gcaaagtatc ttccaaaaag aaaatgccga
     7801 tactccttgt agatacagga tatatcgaca tttaccaact tatgaaaaga taatcaaatt
     7861 taatataagt ttttcaaata aaaagatacc aaattctttt tcgagaatct atataacatt
     7921 acttaagctt ggttggggta taaacaattg agtagctact tttgtatggt gttcaacttt
     7981 agtcaaaact cttaagtaat gcaaatgatt tagattcatt aatattcaat ctcttcataa
     8041 gtagtgaaat tcgagttatt tctgcataat tatactatga tttatagatt aattctatta
     8101 gcatccagaa taatattgtt tatatgagta aaatttaaag aatattgtga aaattatagg
     8161 atgttaaaat aaatttaggt aaaaaagaag tgtttgtata ttatagagta ccttttctaa
     8221 aatatgtggg attgttttaa gaattagaat aaaaattatt tttaaattag tatttttata
     8281 atgtatagta tttttaatgt ataattaaat tgtgcagaaa atagaattta aagtcctata
     8341 gaatttctga ttatggagga gaatatgaaa agaaaagttc aagtaaaaaa tattactatt
     8401 ggagaaggta gacctaaaat ttgtgtacca attattggga aaaataaaaa agatattata
     8461 aaagaggcta aagagttaaa agatgcatgt ttggatatta tagagtggcg agttgatttt
     8521 tttgagaatg tagaaaatat taaagaagtt aaggaagttc tctatgaatt aagaagttat
     8581 atacatgata tccctttatt attcacattt agaagtgtag tagaaggggg agaaaaatta
     8641 atatcaagag attattatac tactttgaat aaagaaattt ctaatacagg tttagtagat
     8701 ttaatagatg tagagttatt tatgggagat gaggtcatag atgaagtagt taattttgct
     8761 cataaaaaag aagtgaaagt aattatatct aatcatgatt ttaataaaac tccaaaaaaa
     8821 gaagagattg tatctcgttt atgtagaatg caggaattgg gcgcagattt acccaaaata
     8881 gctgttatgc cgcaaaatga aaaagatgtg ttagtgctac ttgaagctac aaatgaaatg
     8941 tttaaaattt atgcagatag accaataata acaatgtcaa tgtcaggaat gggtgttata
     9001 agtagattgt gtggtgaaat atttggttct gcattgactt ttggagctgc aaaaagtgtt
     9061 tcagcaccag ggcaaatatc atttaaagag ttaaattcag tacttaatct attacataag
     9121 agtattaatt aaattaatta tatagaaata ttagacggta tttttgaaga tataaattaa
     9181 atataaaaaa cttttcagat agtaaaattt aaattgggca attatatctg aaaagcaact
     9241 atttagtgtg tattttgaaa gtgatataga aaatcttata aatcaactat ggcatattta
     9301 aaatatgcca ttaaaaccta ctagacatta gattatagat ggtatactag catttctatt
     9361 atttatagat agtaagtttt cataataact gttttatcta aagtagaaga tatatttgta
     9421 ttatgtatat ttgtcattat ttttttgtaa ttatgtatat taatggttat atatgtaata
     9481 taatattatc actctatttt ttgtatattg tacattacaa catttatatc tatcatcatc
     9541 aattacagta gaatgtatgg gatacttatt ttcacttaac caatattttt gcatattctt
     9601 tgtccaagac tttggaaact tcaatacttt cttgtttatt ataacatcct catcttcaaa
     9661 aatattaact tctctttcta tttttatttt tctgttgtat atcttattga tagattcttt
     9721 gtcgagtttt atgtctctat acatatcagg gcaatagcaa caaacataat ttcttttttt
     9781 tcgacgttta aatttcacaa ctttctctat gctaatttct aatttttttg taccatcttt
     9841 tataaattta atatcttcta tgttctcgct tgaaaaaatt tctgtcaatc ttaaagtatc
     9901 tacagtttct ctaattattt gctttttata agaagagaat ccatatccaa aagaaaggtt
     9961 taatttatta gggatgtagc catttttata tttaaacatc ttaatttttt ccaattcagc
    10021 attatcttta aatttcaact tttcatacct aaaagttact ttgctaaaaa gagtatattc
    10081 tacatcaata tattcttttg caatcttaaa tttatcgcta ccttcaatca caatcatagc
    10141 ctatctccaa tcgttttata ttaaaataag gccatagaac atggtctaat agtactcatt
    10201 agcactataa gacaatgtta acacaaaaag acaaaaaaag gttatttatt tcaaataacc
    10261 ttttttttta tataaaatat aaagaattat attttattct tttttatgat gtctataatc
    10321 tgtgatatca gatttagttc tcttgaattt aagtctttta actcttcatt tatatcattt
    10381 tctaaatatg tttttccagt ggaagtaata tcttttataa gaacatctgc agaaatattt
    10441 aatatattta caaattttat gaatgtttta agacttggat tctttttacc actttcgatt
    10501 tgagttatat accaaggcga tactccaacc atatttgcaa attcttttga agttaaattt
    10561 gcttttttcc gttcttcttt aagtctttta cctatataat tcattattgt tactatttcc
    10621 ctcacttata tttctttgta tttaatgatg cactttatta actctagcat tgcaatttgt
    10681 tctttttcag ttaatttatt aatcatttca taacattcta aaggtacaga gttatcaacg
    10741 ccttctgttg taataatatt tgcaaataac aattcaagtg gtatgtctaa agcagtagca
    10801 attttcatta taatattgat agatgtattt gtctcagctc tttcaatctg accaatatat
    10861 gttggatgta aacctgcctt ttctgctaga aactcttgac tatatcccaa ttttgttcta
    10921 tagactttta ttctatctcc tattattttt cctatatttg ccaattatta tccctccact
    10981 ttagattata ttcatagttt aagttgtttt atatagacta tttatacttt tttagtctaa
    11041 atatagacta tatatgtttt tatactgaaa taagtgctat ttatttttgt atagtctgta
    11101 tctacaataa acaaatatgg ataaatcata tttttaatta gagaaatatt ttcataattt
    11161 catagccata ctacaataat aaaacgaatt aataatatgt taattatagc attttttatt
    11221 gtaattacat attagatatg tagaatatat tttgaaaatt aaaaaaattt tctattttat
    11281 atattgaaca atttatgtga ataga
//
LOCUS       DAMJYX010000104        11096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJYX010000104 DAMJYX010000000
VERSION     DAMJYX010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.108"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            76..384
                     /locus_tag="PC479_002929"
     CDS             76..384
                     /locus_tag="PC479_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805455.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(546..2336)
                     /locus_tag="PC479_002930"
     CDS             complement(546..2336)
                     /locus_tag="PC479_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805456.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2336..4063)
                     /locus_tag="PC479_002931"
     CDS             complement(2336..4063)
                     /locus_tag="PC479_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805457.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4308..4574)
                     /locus_tag="PC479_002932"
     CDS             complement(4308..4574)
                     /locus_tag="PC479_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2805458.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            4872..5984
                     /locus_tag="PC479_002933"
     CDS             4872..5984
                     /locus_tag="PC479_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2805459.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(6348..6857)
                     /locus_tag="PC479_002934"
     CDS             complement(6348..6857)
                     /locus_tag="PC479_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2805460.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(6859..7506)
                     /locus_tag="PC479_002935"
     CDS             complement(6859..7506)
                     /locus_tag="PC479_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805461.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(7520..8692)
                     /locus_tag="PC479_002936"
     CDS             complement(7520..8692)
                     /locus_tag="PC479_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2805462.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            8960..10336
                     /locus_tag="PC479_002937"
     CDS             8960..10336
                     /locus_tag="PC479_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805463.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(10586..10945)
                     /locus_tag="PC479_002938"
                     /pseudo
     CDS             complement(10586..10945)
                     /locus_tag="PC479_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT         3900 a         1814 c         1320 g         4062 t
ORIGIN      
        1 atttatgttc tttgtaaaat tttttagaag cttctaatgt tatgctataa aactatcttg
       61 agaagggctg attgaatgtt taataaaata tatgaaaaga aagaatttat agtttttcaa
      121 gttaaaaaag gttatgtagt ttataatact agaaagagtt ttgaagaagg tcatacacat
      181 ttaaagcatt ttgaagcagc aaagacagct attgatttag caataaataa aaagattcct
      241 agaagcaaag atggatatta tctgactagt ttaattcgca taagtgatga tggttattac
      301 attgataagt taagcgaatt gttatatgtt agagaacaaa aaggtaaaaa agaaaagtac
      361 tgtaattctg gttatcagat gtaacaagac tagttgtgaa ttataagtac caatcataag
      421 tacctttagt cttaaaatca aagagtatca gtaaatctat aaaaaaacaa cttactgata
      481 ctctttttaa ttatatgatt aatatttgaa tttgatacaa aattttatac aaaagtttat
      541 gttatctatt ctgattcttt gtctgcaaat tggctattat aaagcttctc ataaaatcca
      601 cccttttcca ttaattcatc atgattcccc tgctctataa tacttccatt attcataact
      661 aaaatcaagt cagcattgcg aattgtagaa agtctatgtg ctataacaaa actagttcta
      721 ccattcataa gatttctcat tgctttttga agcataagtt ccagcctagt atcaacagaa
      781 ctcgtagctt catcaagtat caatattgaa gggtctgata taaaagctct agctatggtt
      841 aaaagctgtt tttcccctag agatatatta gatgcttctt catttaaaaa catattgtat
      901 ccatctggaa gagtggttat aaagtgatga acatttgcta cttttgctgc ttgtataatt
      961 tcttctttag tagccccaaa tctaccatat tctatatttt caaaaatggt tccattaaat
     1021 aaccatgtat cttgaagcac cataccaaac attgaacgca aatctttacg tttcatatct
     1081 cttatatcta ctccatcaat tttaatagca cctttcttta catcataaaa cctcatcaat
     1141 aagtttatta atgttgtttt acctgctcca gtaggtccaa caatagctac catctgacca
     1201 ctttttactt ctgcatttaa atcttctatt aaagttgaat tttctccata tccaaaatta
     1261 acatgctcaa atgtaacatt tccttttaca ttttctatct taactggatt ttcaacatca
     1321 ggtatttcct cttcttcgtc taaaatttca aatacacgtt ctatagctgc aaaagaagat
     1381 tgtattactg tagataactg agtcatttgt gaaagtggct gatttatctg ccatatataa
     1441 cgcacaaatg cttgtaaatt tcctactgtt attcctccac ttactgcata tatagaaccg
     1501 acaactgcca cagctgcaat tccaaagtaa gttactaatg aaaccatagg attcatcata
     1561 cttgatataa attgtgcctt aaatccattt tcacaaagtt cttgatttac ttttttaaat
     1621 tcttttatag aatcttcttc tttaccatat agttttattt cattaaatcc agtatacatt
     1681 tcttgtactt ttccatttaa ttttccaaga gcattttgtt gcaatgaaaa tctactttga
     1741 gattttttta ctacaaattt agatacaaaa taactaattg gaattataaa aagagctata
     1801 agagccatat atatattaat tgtaaacatc attgaaagtg ccaaaattat gactaagatt
     1861 gagtttacaa tttgcataaa actctgctgc aatgcatttg atattgtatc tacatcattt
     1921 gatatacgac ttaatacatc tccaaaacta ttactatcaa aataactaat aggcaatctt
     1981 cttattttat tttcgacttc atttcttaaa tctctcattg tgttttgaat agcattcgta
     2041 agtagaaaac ttgaagtata cgtaaaaatt gcattggcaa aatatataca tgctaaaatc
     2101 accaatattt ttgttacata atcaaaattt acggaagccc ctggtactcc ttttgcgata
     2161 ctaattacat ctttagtcaa ttgtgttatt atcaatcctt cagttttagg tgcaagtgca
     2221 ttcatagtag ctgctgaaat tatacatata atagctataa taaaggaaaa tttatatttc
     2281 tttacaaatg gaagaagtct accaattgtt ttcttaaaac ctttagactt tttcattatt
     2341 ctaattcctc cttttttaac tgagatgttg caatttcata gtatatttca cagtttttta
     2401 ataattcttt atgagttccc attcctacaa cttgtccttc attgagtact ataatcttat
     2461 cagcatcaat aatagaacta attctctggg caacaatcaa tacaattgca tcttttgttt
     2521 ccttcttcaa tttagcacga agtattgcat cagttttaaa gtcaagtgca gaaaaactat
     2581 catcaaaaat gtatatttca ggtcttctta taatagccct tgcaatagaa agtctttgtt
     2641 tttgacctcc tgatacattt gctccacctt cactaatcaa ttcatcaaac tttctaggtt
     2701 tttttgatat aaactcatat gcttgtgcaa cttttgcgga atattctaat tctgactctc
     2761 ctgccttatg tttcccaaat ctaatattat ttgctatgct accagtaaaa agcagtgatt
     2821 tttgaggtat gaatcctatc ttttttctta aagcttttaa atcatattct cttatatcta
     2881 ctccatttat cttaatgcta ccttctgtta catcataaaa tctaggtatc aagttaatta
     2941 gagtactttt cccactacca gtacttccta taaaagcgac tgtttcacct gtttttgcag
     3001 taaatgaaat atccttaagt acagaagctt ctccatctgg ataggtaaaa gttacattat
     3061 caaattctat gataccctta ttttcagtat ccttaattcc attttcagga ttttttatca
     3121 atggttcttc atttaatagt tcttttattc tatttgcaga tacctctgct cttggataca
     3181 taataaatac cattgaaaaa agcataattg aaaacatggc atggaaaaga tattccaaaa
     3241 atgcaaccaa ctgtccaact tgaagctctc caatatttat cttctcactt gaaatccaaa
     3301 atacagccaa aacagctata tttaatagta aaaagaatgc tggttgagta attgacataa
     3361 gcttaaatag tttcttagaa acattagcat aatatgtatt tgttttttca aatcgctctg
     3421 tttcataatc atcatttcca aaagctctaa ttactcttat tccagttaaa ttttctctcg
     3481 atataaggtt taatttatct agtccttttt gttgttttga agatattgcc tgagatactt
     3541 ttgctattat tgctactcca ataattataa aaggtactga tatagccaat actatagaaa
     3601 gttccacact tgttctaatt gtcattatta tacttataat gaacataact ggagttaata
     3661 aggcagtcct tagtagtata ttaacaaatt gttgaatttg aaatgcatca ttcgttgttc
     3721 ttgtaatcat tgaagaaatt ccaaatttat tgtattcagt atgtgaaaat tcttgtgatt
     3781 tctcaaatat gtcatttctt atatctctgg ttacacttgt tgatacctta gcagaacaat
     3841 aacccaacaa tatactacct acaaccccta taatagaaat aataacaatc actattccca
     3901 tttgttttat gtaagctata ttctgatttg caataccatt gtcaattatt ttagctatta
     3961 tagttggaat ccccaactcg actaatgcaa aactaaatat agccagtatg tttaatacta
     4021 ttaataactt ataattcttt aaataactta aaattaattt catatttttc tcctttctta
     4081 atttttgaga tagtgtaatc atgtaataat actaaattta taaatgctta attttataaa
     4141 aaaaggagaa aagagttaac gcactctctt ctccagcata ttctttttaa tacaccctcc
     4201 gacaagtcgc ttatatgtta acatatttga caagtaatgt caattcatat agccaaaagt
     4261 cttttaaatg cttcgtttca taacattgta acaaattggc atgtaattta tttttgtttt
     4321 ttactgtatt ccaattttac aagttctcca atattactag ctttcttaaa attcaaatta
     4381 gtcttatcta taccagactt aaatagtgaa atccctctcc ccaatattat tgtcaatata
     4441 tatatataat aatcatcaat taagttatct ttcataaata attttactat atcgcctcca
     4501 ccaactatcc aaatatcttt tttatccttc tcttttccaa tttgctcaat taatttttta
     4561 ggttctaaat taatttatag tgtgattatt ctatgtaaac atcacttttt tactcattca
     4621 ataataccct attaataaat cctcaaacta atttaaataa actttgtata aaattaaaaa
     4681 tatattatat aaataattta aaactacact aacttattga aaattctaat tattacaaat
     4741 aaatattgct ctctgttaac gctagttcta taatataatt ataagctatt aatattataa
     4801 agccgatatt actaatataa taattataat acttatagtt aatttacttt tgttgaaagg
     4861 tggtaaataa catgaaaatt tcagaagtcg ctactccaaa ttttttgcta gatttagacc
     4921 agttagaaaa gaatatagat aaaattcaac aaatttgtac aaataataat aaacaacttt
     4981 ggcctatgct taagacacat aaaagtactt acatagcgag acttcaaaaa aatgcaggtg
     5041 ctagtggttt tctagttgga acacttgatg aaggtgaagc tctgataaaa gcaggttttg
     5101 aagatattat gttggcttat ccttacatgg gagaagtaaa tataaataga attgtgacta
     5161 tggcatcaaa aataaattta atctgtgcta cagataatat agaatgtgct aaggcttata
     5221 gtgaagcttt ttctaaatca aaaatagagt gcaagctact tattattgta gattgtggct
     5281 tacaccgttt tggggttgaa ccagaaaaag tggttgaact tgctactgaa atttctaagc
     5341 tagaaaacat caagattgtt ggtataagtt cgcatccagg tcaagtgtat ggttgtagtt
     5401 ctccagaagg tgttccagat gtttgtgttc aagaagattc ttctatggag aaagcataca
     5461 aagctctaac tgataatgga tttaatatag aaattgtcgc ttctggtagt actccaacag
     5521 ttgaatctga agctgcttct aaaactataa ctgctgtaag acctggaaac tatgtatact
     5581 atgatgctat acagataggt ttaggttgtg ctactgaaga tatgtgtgct cttactgttg
     5641 tgtgcactgt aatctcaaaa aatagcagtg gatactattt aattgattgt ggaagtaagt
     5701 gccttggact tgataaaggt gcacatggaa atagtagtat tgttggttat ggtagggtat
     5761 gtggacatcc agaacttact attgattcac tttcagaaga agttggaaaa attaaagtaa
     5821 atggtgaaac tgatataaaa attggtgata gaataagaat tatacctaat cattcttgtt
     5881 ccactgcaaa cttgacaagt aatttattag gttttagagg agatacaata gaaaaagtca
     5941 tagaagtaga tattcgtgaa aattcaaaac aaatcatcct ttaaaaacac atttattctg
     6001 atattaacat gaaagtatat ttttcttaaa attattgcta ataactaatg ttaatgttgg
     6061 ataaataaaa aacataaatt ctagaaatat aaatttaaaa gaagaggctg tctcaaaata
     6121 gagataagcc tctctttcat tgttgaaatt atgaaaatat acttgatttc atacaaaaag
     6181 agtatctcat gaactaaaaa gttcactttg agatactctc tttttttaat taatatacac
     6241 attaattact atcttagttt attactatat atcaattact actcattaat ccttgtatat
     6301 attaattctt atatatcatt tatagttagg ttataatttt atgttattta cattaatccc
     6361 ataacagtga gtaaaactct tactatcaac atccccacta ttgcatcaaa tataggaact
     6421 gccaacagta acattctata cttagtactg gcatttgcta caagcactat tctggcataa
     6481 tgaccaacta gagtacccat taacatacat ggcataatta atatggttgc ttgtggtgca
     6541 gtgattagac cttgtgcaaa taagtttgca gctgtagctg ctcctgctgc cttagcaaag
     6601 aatgctgcta tcaaaactac aacagattcc ccaggtagtc caaaaatacc cattactggt
     6661 ccaaaaatat ttcctaaaat atcaactaaa ccagttaatt gtaaaaactg cactattaca
     6721 taacccatta tcatggctgg taatatctgt tcaacaccta tgtaaaaacc tttttttgct
     6781 ccccccataa atatctcaac agcagttttc ttaggtttac tatttacaat tacatttcca
     6841 gtattttgtt gactcatact acacagcctc cttagcctta ttctttttat ttcttacacc
     6901 tatgattaat ctaactagat tagcaccaag tattttacag aatatctcta taagtattat
     6961 tggacctaat gccaataatg ctattggtaa aagtggagct tgagtattta ttgtgttcag
     7021 tataacagct gaaccagcat attgatatgc tacaaatata gttctttcat catctgttat
     7081 ttccccactt tcaaataatt ctttcgtcat tattgaggct acatctgaac tagtaaatga
     7141 agatacaaat gctattcctg ctattccagg tataccaagc agaggtctaa gcaatggatt
     7201 aaatatcttt gatgccgctt tcattcctcc catagattca actacatcta taagacctac
     7261 agcaaaacag acagttggaa tcaaagtcag agcaaataag aaaccatctt ttgctcccgt
     7321 tcctccttta cccataaagt ctacaccatc tgcaatagta ccaaatgaac ctgtcagatt
     7381 agcaaaatct aatgctttaa ggaagccttg actatcttta aacactcctg aaaataatac
     7441 tatcaataca aataaagata aatacccttt ccatgttacc ttattatctc cagctgttac
     7501 atccatataa aatccccctt tatacctcaa atttatctaa gtttaaaata actgtagaaa
     7561 ttaatttcac cctctcaaac atagaatcta ctactgcata ttctcttgat gtgtggttcc
     7621 attctcctct tacaccaaat gaacagattg taggaacgtt tgcaatagtt aaataacttg
     7681 catctgaact acctccaagt ctcttagcat ttaattctcc aaatccattt tcttttgaca
     7741 cttcatttac aaatgtatga aatctcataa catcttctgt agtttcaaat ggcatcattt
     7801 cacttataaa attaacttct gtagaagttc cttctatgta agtttcttta catatctctc
     7861 taacttgttc tttaacattc tccatttctt ctactttttc aaatctaagg tctactttta
     7921 tactacaatc ttctggaata gaattttcca ctcttccacc cttaatcaca cttacactta
     7981 ctgtagttcc aacatctaaa ttagtcaatt tttgtatgcg tagtatctta ttagccattt
     8041 cttctatagc atttcttcca ccttcaaaat cattacctgc gtgtgtttct acacctttta
     8101 catgtatatt acatcctatt cttccttttc tacctacaca caaactatta tctactaatc
     8161 cagtttccat attaaaagca caaagaccac cttttgcttc cctcaatata acatctgcac
     8221 ctgttgaatc cttatgtcct atctcttcat ctccagaaaa tactattttt attggtcttt
     8281 ctttgtatcc tattttattt aatgctttaa tagcatataa ggaaattatt ataccaccct
     8341 tcatatctaa aacacctggg ccatatgctt ttccttctat tattttaaat ggattctctc
     8401 caaatgtacc tgtttcaaag acagtatcca tgtgacctga aaaaattatt ggctttttat
     8461 ctatattgga accaagtgta cctatcaatg tactaccatt atctccaaca tctactaact
     8521 cgcaatctaa accttctttt tcaaactcaa actttaatct cttagccaat ttatttacgc
     8581 tttctttaca atgtgtatag ctttccatat taacaatctc tttccataat gaaactattt
     8641 cttctctatt ctcatcaaca aacttagaaa tttcatttaa atatgtattc ataatcttct
     8701 cctttatttt caaataaaat gtatttcttt cctaaaatct aagtttattt taggaaatct
     8761 taaaagacta gtccttaatt acaaaatagc acaattattc ttgacagtca acatattttt
     8821 cagtcttttt tatattttct gatttttctt tcaattttcc ttttgtaaaa taaattgacg
     8881 cgttttatct actattataa aatgaggtta taaattgatt gattagttac aatactctcg
     8941 aaattttaaa ggaggtttta tgaaatataa aattggtttt ttgacaacat cttattttat
     9001 gtcagataag tttaaacaag ctcttaaaga attaaatgat acttgtgaaa ttacatttat
     9061 tgagcttaaa ggggagaatc cttttaataa tttacctaat atatactatg agcaagtaga
     9121 taattttgat ggatttgtat gcagtggtat agttccatat agtgaactta taataaatat
     9181 aaaagacata aaagttccat taaatttttt aaagcttgat gaaaaagatt tttataaata
     9241 cttattcaaa ttattaaata gccaaaaaga tattgacttt acaaaatcat ttatggattt
     9301 tttaagagag gacaacaatt attatgatat atattcatta atagactcta ataaatgtcc
     9361 atatacaatt aaagatttta atatccctca acctgtatat gactttaaaa aattaaatga
     9421 taaattattg gaaatacatt tagacttatt gcgagataat aagattaacc tttcttttac
     9481 tagaaattac atcattagtt gtgaacttaa taaacttgga tatgattcta tttacatggt
     9541 tccatctaca gaatcaatcc tgaatacatt taaatctctg gtgaatgaaa taacattaca
     9601 aaacttagac aaaaataaat ctgcaacttg tatagttaca gtaaatagtt cagagtacat
     9661 aaatgaagat ttgatattta aaaatgatga gatacaaaat caaatatata acactatatt
     9721 aaattctcta tcaagacatg gattctatgg agttcaggtc aaaaaaaatg atatgaaaat
     9781 agagatacac actactaaag aaatgttaaa caatatgacc aataactata cagatttttt
     9841 tatttcagaa gatttaaaag aaattaaata tagcttaaat ataggttggg gcattggaaa
     9901 tacaaatatt catgcagagc aaaatgctcg tcaagctaat ggtaaatctg cttctttgaa
     9961 tggaaactgt acttttatag taaatgatac taatgataca ataggccctt tatattcaaa
    10021 taaaaattct aataatattg aagatagctc aatagccaat aaagttgctt cttttatacc
    10081 tctatctaat acaaatgtat ctaaaatcat gtgtatgatt aatgatagaa attcaaataa
    10141 tgtgtcagca gaaatattag cagactatat gaacataact cttagaagtg ctaatagaat
    10201 attgagtatc ttatataaag caggaattgc aactattgta aatacaaaac tcgataatca
    10261 aagaggaagg ccaaaaaaaa tatataaagt tgatttctta tcttttctaa ataagatgca
    10321 gaaattaaat tcttaatatt taatttttta ataaaagcaa tgtattttct ctaattttaa
    10381 agaatcaaaa tgtttaaatt ttattagaat atacaacttt ttactttaaa atacctaaac
    10441 cttttatcag tgaaataaac cataatatat cgtaacttat tagaaaaaca aaataatttc
    10501 atatacaaat aataaaatag ccactacaat ccataatttg cagtggctac tttttacact
    10561 atcacttatt acttattttt aaaaattaat tgtatttttt aaataattta tacacaccta
    10621 ttatcagtaa agcaataaat acaagtatat ctataaaaat aattattgta acatgtctga
    10681 caatattgta aataaaaaat atagagaata tttctataga caacgctaca aaacacataa
    10741 aattcgttaa ataatctgca atagtatcac tataaaaagc atgtatttgt aaccatctcc
    10801 atataaatgg acgtaaaagt attctgaata caacacaaat gacagggtag gcataaataa
    10861 attttttatc tacataatta gctacaccat tattccattg gataggaatt tgaggtggca
    10921 acttttgaaa tactgttatt gcaatttaaa cactacctct ctattctatt aatttataac
    10981 tataatatag tcttaaatat tttcactcta taaaattgta tatttagtat atcttaaaat
    11041 caaactaatt atcttttata agttttcata caataatatc tcctttcaat aatata
//
LOCUS       DAMJYX010000105        11046 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJYX010000105 DAMJYX010000000
VERSION     DAMJYX010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.109"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            6..1487
                     /locus_tag="PC479_002939"
     CDS             6..1487
                     /locus_tag="PC479_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2805464.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1520..4954
                     /locus_tag="PC479_002940"
     CDS             1520..4954
                     /locus_tag="PC479_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2805465.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            4972..5718
                     /locus_tag="PC479_002941"
     CDS             4972..5718
                     /locus_tag="PC479_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2805466.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5667..5975)
                     /locus_tag="PC479_002942"
     CDS             complement(5667..5975)
                     /locus_tag="PC479_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2805467.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            6779..10921
                     /locus_tag="PC479_002943"
     CDS             6779..10921
                     /locus_tag="PC479_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2805468.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         4462 a         1330 c         1752 g         3502 t
ORIGIN      
        1 gctatttgaa aaaaaataga ttaatagcaa agaatatatc aatagcattt atagtactat
       61 tcttttttag tgtatttaca tttttttatg taggtaatat taatagagtg ttagaatatg
      121 aaacaaatga cataataaca gttacaattg ctggatggat tatactttca tttttatttt
      181 taggtattat aatttacata ttatattcaa aagcaaatag tcaaaaaact atagaaaaag
      241 tagcatatac ggattttgta acaggttatt ccaactggag aaagtttgaa ttagatgtta
      301 caaatttatt aaagaaaaca tcacaaaata ataaatatgc aatggtcata ttcgatattg
      361 ataaatttaa agcaattaat gatatatatg gccataaaaa gggaaattta atattaaaag
      421 atattgcaga tactttaaat gaattgactg atatcaatga aacttttgct cgtgtaagtg
      481 ctgataattt caatatatta ttaacatata acaaaaaaga ggatataata aatattatca
      541 aaaagattat ggcaaataat gaacttgtaa acttatcttt tggaatttat gaaatcaaag
      601 ataaggattt atctgtaagt gtatatagtg atagagcttc acttgcaaaa tcatcaataa
      661 aaaataatag tgatgtaaat tttgcatttt ttaatgacaa attaagagag aaattattat
      721 ttgaagataa gatagaaaaa gaaatggaat atgctcttga aagtgggcaa ttcgtaatgt
      781 atttacaacc aaaatacaat attaaattag ataaattttg tggaagtgaa gccctagtca
      841 gatggcaata tacagaaaaa gaggtcatat atccaggtga ttttattcct atatttgaaa
      901 aaaatggatt tattagaaag attgatatgt acatattaga acaagcttgt aaagaaatcc
      961 ggtctttatt tgataaagga atttcacctt tacctatatc tgtaaacttc tcaagagttg
     1021 atttttttaa aaaagatttt attgaaaaca tagtaaatat atgtgataga tataaaatac
     1081 catatagttt aatagaaatt gaaattactg aatctagtat gtttggagat actgatactc
     1141 tatttaatgt tagtagaaac ctacaggata ttggctttat agttgcaatg gatgattttg
     1201 gctctggata ttcttctgta aatatgctta aaaatatacc attaaatgta ataaaattag
     1261 atagaggatt ttttgttgat gataaagatg ttgataagag tcaaattgtt ataaaaagca
     1321 tagtttctct cattaagcaa ttaggaataa gggttgttgc tgaaggaatt gaaacaagaa
     1381 gtcaaataga aatgttaaaa aaagcaaatt gtgatattgt tcaaggatat tatttttcaa
     1441 aaccattacc aattaaagaa tttgaaaaat tagtctataa aatatagtca gcgagtctta
     1501 aatgggaggt ggtgattaag tgataagagc aaaatcgtta aaaaatgtac tgagtattgt
     1561 tttaatattt ttatttacaa taatttcttt ttctgatttt gcatttaaaa ctaatgtcca
     1621 ggaagaagaa aatagtaaaa ttaatttaag agaaatatcc ctacaaagtt ccaatgctat
     1681 aaaaataaaa ttagatagtt ctctatcctt tattaaaagt gtggcaaata tggtttctta
     1741 ttttaatact atagataact caaaaatagt tccattatta gctaatatat caaaagaatc
     1801 tgactttcaa agaatctcta ttgtatctcc tgatggaata agtcatacca gtgatggcaa
     1861 aattatcaat gtgtcaaagt atcagtattt taagaaagct attaaaggag aaagtaatat
     1921 atctgaagtt gtgaaatctc caattgatgg aaaagatact tttgttatag ctactcctgt
     1981 atttaataag aataacaaaa taattggtgt tttatacggt tcttactaca atgataaatt
     2041 agctgaattt atagatataa caagttttaa tagtgaaggg tacatagata tatttgaatc
     2101 tagtggtcaa tttgttttaa aatcttcaca tacaaacaaa ctgattaagt caagtagtaa
     2161 catatatgat atatttaaaa aagcttcttt tgatgatggt tatagctata aaaaaatata
     2221 tgaagataca aaaaataaca agagtggctt tacctcatat gaatacaaag gagaaaatcg
     2281 ttatgctact tatacaccca ttggtgtaaa tgactggtat gtaatttctg tgataccaca
     2341 atatactgta agaagaaaca ttgataaaat aaatcaatta gcattttctc ttattttcaa
     2401 ggtattctta ttgtttatta tgcttataat atatattatt tataaaaaca ataaatctca
     2461 gaaagatata ctaaaagcta acaaagaaat ctcatctatt acaaatagca ttcctggagg
     2521 agtacaaaag tgtaccctgg gtgaaacatg caatattaat tttttaagtg atggatttat
     2581 agagttgttt ggttatacaa gagaagaaat aaaaacattg tttaataatg atttttatag
     2641 tttaatacat aaagatgatg ttgaaagaat aaaaaaagaa atttttaaac aactttacaa
     2701 aaataaaact gttgaattag aatataggat tataacaaaa aatggtaata cagtatgggt
     2761 tttagataaa ggggagctta taacagaacc tgatggaaaa gaatattttt gttgtgtaac
     2821 tgtagatatt actagaacaa aacaattaga acaagagatg gaacaaaaaa atctagaact
     2881 tgaaatgata tcagaaaatg ttttaggtgg tataattata acagaattta atgatgattt
     2941 tactataaaa tacgctaact ctggttatct ttcaatgatt ggatatacac gtgaacaatt
     3001 agaaaatgaa cttaaataca aagcttgtaa tcttgttttt tatgaggata aaaagaaggc
     3061 tcttgaaagt atacaacatc aattaaacac aaaaggaata gctgtatatc aacatagaat
     3121 aagaagaaga aatggtaata ttatttgggt ttctataaaa ggaaggctta ttgttgatga
     3181 tggtattgaa aaaggtattt ggattctaaa tgatataaca gatttaaaaa atgcagaaca
     3241 aaaacttaaa ataaatgaag aaagatttag aattgcatta aagcaaacat caaacactat
     3301 atttgagtat gatataaaaa caaagtcact tttaaatact agtaaagagc ttgataaata
     3361 taatatttat aaaattacta aaaacgttcc atattctcta gtagaatcag gagttattca
     3421 taaagatttt agtgatgatt ttataaacat gtatgaaaat gtaataaatg gagcaaaaaa
     3481 agcaagttgt gtagttaaga tgaaacttcc agatgaaaat tatatatgga ataaagttac
     3541 cttgactact atttttgatg aagatggtaa tcctgtaaga gcaattggta tacaggaaga
     3601 tataagcaaa caaaaggaag cagagataag atacaatcaa gaaggtaagt atcattcagc
     3661 tatattgtcg gaagcaatga taacttatga aataaatgta tcaaaggata cttttatcaa
     3721 aggaaatgaa agttggcaaa aaccatttgg aattaagcct gacaataact attctcaaat
     3781 ggtaaaaact atagcaaata gtattgtaca taaagacaat aaagaaacat ttttaaatac
     3841 atttttatgt gaaaacttat tggatgcata taaaaataat gtgactgaaa taaaattaga
     3901 atatcgaaga atgaatcaag aaaataaaat gatttgggtc atttgtacaa tgcacttaat
     3961 aaaagattct attacgaacg atataaaagc acttgcttat gttaaagata ttgacaagca
     4021 aaaaaatgaa gaaattagat taaaattaaa agctgaaaga gatactctta caggattata
     4081 taataaaggt actacagaaa aattgattaa tgatttcttg aactctgagg aagcaagctt
     4141 aggaatgcat gcatttttaa ttgttgatat agataatttt aaatctgtga atgataattt
     4201 aggacatgta tttggagata aagtattatt tgaagtttct aaaaacctaa aacctatatt
     4261 tagaaaagat gatatagtag gtagaattgg tggagatgaa tttatagtat tcttaaaaaa
     4321 tattcagtct tttgagaata tcaaaaagaa agcagaaaaa atatgcgata tatttagaaa
     4381 ttcttataca gaaaataaaa agacatataa aatatctgga acagttggaa tttcatttgt
     4441 accagaacat ggaactgtat ttgaagaact ttataaaaaa gcagacatag cactttatta
     4501 tgcaaagagc aaaggaaaag ataagtatgc actttataat gattgtatct accaaaatga
     4561 acttgaaaat aatataagtg ataataatat aaaattagca gataaatgtg agaaatccaa
     4621 accagtactt aaagaaattc ttgattctat tgataattat atctatatag tgaatccaaa
     4681 tacttatgaa cttctttttg tcaataataa ggtgtctcgt ttggatttag agataaaatt
     4741 aggagaaacc tgttataaat cactattaaa atctgataga ccatgtaata attgccctct
     4801 taacggatta gaagaaaata agcattcaaa attcaaaata gagacaaata catttggtaa
     4861 aaatatcagt atagcaagtt ggattaattg catagatgga gaatacaact gtcttataga
     4921 gtgtatagat tcttctttat acaaaaataa ataaaaaagg actatgaaga tatgttaaat
     4981 agtataactt tgatacttgg tataatattt acaatttact atttttatct caaaatagtt
     5041 tttggaggaa tttcatttag tgagattttc ttagttgtag gaattgtact aattatctat
     5101 caaatattta agaaaaaaat taaagaaaag aaggtttttt atagggtgtt aaaaataata
     5161 atcagcattt ttttaatagt atttgtagtt actgagagtc taattatttt ttatcccaaa
     5221 aatagtttag aatcaaaaag tgattatctc ttaattttag gagcttctgt aaaaaagact
     5281 acaccaagta caaccctgaa gggtaggtta gacactgcat tgaaatacct aaaaattaat
     5341 gataattgtt atgtggtagt atctggtggt aaaggaagtg gagaaaaaat aactgaggca
     5401 aaagctatga aagattatct tataaaaaat ggtatagata aaaataggat aatagaagag
     5461 gataaatcca cgaatactta tgagaatttt aaatattcaa aattgaagat agaagaacat
     5521 agtcaaggaa aactaagcga tttaaaagta aaaatagtaa caacagattt tcatgttctc
     5581 agaagtaaaa tattagcata tagaaatgga tataaaaata cgtcttttta tgctagtaaa
     5641 tcaaaacttt catttgtacc aacttactat acaagagaat tttttgcatt atggaaaaca
     5701 atcatttttg atagataata aataaaaaga ctagatataa actcatatct agtcttttct
     5761 attcccttaa ttctaatcca ttttccaact ttacttgttg tagaaatttc aacattggga
     5821 tttacaagta attgcttata agaagtttta tattttacga ttccaaaata taattttcct
     5881 tcataatcca ttataaatcc gaatggtctt actcttggtt tattatcttc aactgtagca
     5941 aaatagaatg ttgagttttc tttaaaaaaa ttcattattt tttcatgatt aaatcctctt
     6001 tgtatcttat actaatataa tgaatataaa taaaatattc ataatactaa tataaatcga
     6061 tataaaaaca attacaatat tatattgatt attcaaaatt atatataaaa ttcagaaaaa
     6121 aattttagtt tacataataa aaataaagtc aactaaaatt attgatttta gattaaagta
     6181 ccggattaag catttaagta gattttaagc ttgatttgat tagaaaggta caaactatca
     6241 ccgaaaaaaa ttaaacccat ttaaaaagca atataaaagc ttgttatttt tgagaaaaat
     6301 agaccctcta aactcaatta gattaaaaat aaaatacaga aaattatatc tctaaatatc
     6361 ttaaataatt aagtttaaat tttacgatat tttgtttgat gatttataat ataaaagcat
     6421 actcttaatc ttcaaaattt aaaaagagta aaaaataaca aaaaaatcac tgacatttca
     6481 ctgacaaatt gtaattttgt tattataaat acattgaaaa tacttgatta ttgaaaaatg
     6541 gcataaaata atacatttta tgccatttgc tattatttga tagtttttaa cttatgacat
     6601 aagatatttt ttaacatata caataaaata taaaaaaatt gatattgcaa ataaaaaaaa
     6661 atacatatta gatgttatag aaaaaaagcg cacttatagg cttaaaatgt cacataaaag
     6721 agttttaaag aataatatat tatgaacaca aattttgtaa tcacaaagga gtgataatat
     6781 ggcaacagta tcaggacaaa tagtttttga tagaaataga agtgctacca ttgattctgg
     6841 agactcagga atagccaata taccagttgt attacaagat atagcaactg gagttagact
     6901 tgtggttctc acagatgcat caggaaatta tgtttttact aatgttccaa atggtaacta
     6961 tagaattgta gaagcttttg gaactacagg aggagtacca acaccaggaa attttgcaac
     7021 agcgatagta ggtccagttc caattgcaga aatgccacct attagttctg tgacaagtcc
     7081 tcctattggt tcaacaaact tagatggtac aacacctaac acactattaa taacagtagc
     7141 aggttcagat ataacaaatc aaaatatatt aaatgggcct gtgatttata cacctataga
     7201 gaatatattg gattcttgcg tatctgtatc taatataaac cttattacag atgcagataa
     7261 cggaacattc ggtttttttc caccaggtac accaagtaat acaggacctg aaatagaacc
     7321 ttacccaaat gtgacgccag attttacgta tgttgtacca gacccaacta agtttacacc
     7381 tttagatgga gaatatacag tacaaaatat tatgacaaat gcaatgagta atgttatagg
     7441 agcttggtgg cgtattgctg accatactac aggaaatgaa acaggaagaa tgatggtggt
     7501 aaatggtttt aatcctggag ctgtattttt taaatcaaca gtacaagtta cacctaacac
     7561 taattattta tttagcactt ggattttaaa cctttttaag gtaacaggat atccaccacc
     7621 acagctaggt gtaagaatat ttgaccaaaa taataatata ctatatcaag caacattagg
     7681 agctcaaatt ccagttaata caaacgcacc agaatggaaa gaagtaggta ctgtgattaa
     7741 ttctcaaaat aataatcaac ttacagtaga attttttagt gaaggagaaa gcgccattgg
     7801 aaatgactat gctatagatg atgtttcttt aagagaaata gctgttccaa cttttactcc
     7861 aataaaaaca agtaacacag aaactgctaa tgtaggtgat ataatcacat atacagttac
     7921 attagataat acttgtgata gccctttaac taatgtattt tttagggatt taattccaaa
     7981 tggtttagag tttatacctg gaactgtaac ggttgatgga gtatctgtac caggagtaga
     8041 ccccaataca ggtttcccac ttccagatat tggaggagga acaggggttg aagtaacttt
     8101 tgatgttgtg gttgaaagta ttcctaaccc aaatcctact aataatatag ctactataga
     8161 ttactcctat acacctgtag aaggcggtat accaaatgat ttttcagtag attctaatcc
     8221 agttcctgtt gaagtaatct cagcagatat tgaagttaca aaattatctg aacctactat
     8281 agtgaatcca ggagaggaat taatatacac tataaaagta gtaaataatg gaccattccc
     8341 atctgaaaat gtggttttaa cagatgatgt accagcttct attgtaaatc cagagtattc
     8401 attagatgga ggagtaactt ttcaaccatg gactggaagt ttaaatatag ggacattaga
     8461 agtaggagag acaagagtta ttataattag gggtattgta aatccatcaa cagtaggaat
     8521 tataacaaat acagctgtag tatcttcaac tacagcagac ccaaacctta ataataatac
     8581 atctacaata gagacagaag tgaatttatt agcagatatt ttagtaatga aaacagcaga
     8641 acctaattca gctgttccag gtactttatt gagatatact attcaagtag aaaacttagg
     8701 accagcaaat gctgaaaatg taatattaaa tgatgatatt ccagcttcta ttataaatcc
     8761 agaatattca ttggatggag gagcaagttt tcaaccatgg aatggaagtc ttaatatagg
     8821 aactttaaac tctggaattt cattaacagt tttaattcaa ggaacagtta gtttaaattc
     8881 ttcagaatat atagtaaata cagcaacagt ttcttcaact acacctgacc cagatttaag
     8941 caacaatata tctaccatta ttacaccagt taacccacaa gcaggtattt ctattataaa
     9001 agtagctgac gaggatgtag ctgttccagg agaagaattt gtatatacaa ttgaaatttt
     9061 taatgaagga ccatctaatg ctacgaatgt agttttaaca gatgatatac cagatgttat
     9121 tttaaatcca gaatattcat tggatggagg agcaactttc caaccatgga ctggaagtct
     9181 taatataggt acagttgcac cagggcaatt aataagaatt atcataaagg gattagtaag
     9241 ttcaactgct acaggagata ttactaatac agcagaagta agtgcagatg taccagaacc
     9301 agttacggat tcatcaacag tgacaactcc tatagttcca tcagcagata tagaagtaat
     9361 aaaaactagc aatatggata cagcagttcc aggagaaact ttcagttata ctataacagt
     9421 tacaaattta ggaccatctg ctgcacaaag tatcgttttg acagatgata taccagatgt
     9481 tattttaaat ccagagtatt cattggatgg aggagtaact ttccaaccat ggaatggaaa
     9541 tcttagtata ggcactttgg atgctggaga aataagaagt attataataa gaggaacagt
     9601 gagccagaca gcagtaggta caattataaa tacagcaaca ataagttcaa ctacacctga
     9661 cccaaatcca gataataata cttcaacaga tgaaacagat ataagtccat tagcagatat
     9721 ttctgtgata aaaggcaacg aaccagttgc tataccaggg ggaagattta tttatggaat
     9781 tgaaattgca aatgcaggac catcttttgc agagaacgta actttaacag ataatatacc
     9841 agcttctatt ttaaatccag agtactcaat agataatgga gtgactttcc aaccatggaa
     9901 tggaagcctt aatataggta ctttagatgc tggagagata agaaatatca taataagggg
     9961 aacagtaagt caaacagcaa taggcacaat tataaataca gcaacagtaa gttcaactac
    10021 acctgaccca aatctaaata acaatatttc aacatcagaa gcagaggtaa gttcatcagc
    10081 agatatttct gtagtaaaac gaagcaatca gactgttgtt gtaccaggag atgtactaga
    10141 ctatacaatt gaggtgagaa atgcaggacc gtctactgct cagaacgtaa ctttaacaga
    10201 taatatacca gcttctattt taagtccaga atattcaata gataatggag tgacttttca
    10261 accatggaat ggaagtctta gtataggcac tttagatgct ggagagataa gaaatattat
    10321 aataagggga atagtaagtc aaacagcaat aggtacaatt ataaatacag caacagtaag
    10381 ttcaactaca cctgacccaa atccagataa taatacttca acatcagaaa tagatataag
    10441 ttcatcagca gatatttctg taataaaatt agcaaataaa acagaagcat gtgtaggaga
    10501 gcaaatagat tttgtaatag tagtatcaaa tgcaggacca gaaaatgctc aaaatgttac
    10561 tttaatagac aatgtttcag ataagcttaa gaaagcagta ttttcacttg atagaggtgt
    10621 aagtttccaa ccatggactg gaagtcttaa tataggtaca ctaccagctg gaactttaag
    10681 agtgattata ttaagaggaa ttataaaatc aacatgtctt gatagattaa caaatattgc
    10741 agaaattact tcaacaacac cagattcaaa ccttaacaat aatatatcaa gagtgcaagt
    10801 agaaataaaa caatgttgtt gtaatgattg ctgttgtaac aactgtagta attgttgttg
    10861 caatgattgt ggttgtaaag attgttgctg taattgttgt aaaaatgact tttggtgttg
    10921 attttataaa ataattttgt ataccttaaa ttaaactgaa ataaaaaata aattcacata
    10981 tgagtaaaat attttataaa tattaataaa ttttctatct ttgatatgga ccacattcgt
    11041 aatact
//
LOCUS       DAMJYX010000106        10793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJYX010000106 DAMJYX010000000
VERSION     DAMJYX010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.110"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            74..841
                     /locus_tag="PC479_002944"
     CDS             74..841
                     /locus_tag="PC479_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2805469.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1059..1272
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1526..2560
                     /locus_tag="PC479_002945"
     CDS             1526..2560
                     /locus_tag="PC479_002945"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2805470.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2601..3818
                     /gene="argJ"
                     /locus_tag="PC479_002946"
     CDS             2601..3818
                     /gene="argJ"
                     /locus_tag="PC479_002946"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2805471.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3833..4693
                     /gene="argB"
                     /locus_tag="PC479_002947"
     CDS             3833..4693
                     /gene="argB"
                     /locus_tag="PC479_002947"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2805472.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4723..5910
                     /locus_tag="PC479_002948"
     CDS             4723..5910
                     /locus_tag="PC479_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2805473.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6019..7041
                     /gene="argF"
                     /locus_tag="PC479_002949"
     CDS             6019..7041
                     /gene="argF"
                     /locus_tag="PC479_002949"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2805474.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7168..7785
                     /locus_tag="PC479_002950"
     CDS             7168..7785
                     /locus_tag="PC479_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2805475.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8005..8703)
                     /locus_tag="PC479_002951"
     CDS             complement(8005..8703)
                     /locus_tag="PC479_002951"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2805476.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8865..10082)
                     /locus_tag="PC479_002952"
     CDS             complement(8865..10082)
                     /locus_tag="PC479_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2805477.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10273..10686)
                     /locus_tag="PC479_002953"
     CDS             complement(10273..10686)
                     /locus_tag="PC479_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805478.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         4223 a         1182 c         1805 g         3583 t
ORIGIN      
        1 tattcaaaat attaagataa aaatatatag aatatattta aaaattatta tgcaattaga
       61 aaaggagata agtatgcaga ttattgatat aaatataaat aatattgaca atgaacatat
      121 atgttgttca atttctgaaa aaaaaggaga aaattgtgtt ttttctaaaa aagcttggat
      181 gaaaaataat tttgataaag gtctagtctt taaacgattt gatgtaagag gtaaagcttt
      241 tatagaatat attccagcag aaaatgcatg gtgtcctatt actgcggaca attatatatt
      301 tattaactgt ttttggatat ctggaaaatt taagggtcaa ggatattcta atctattgct
      361 acaagagtgt gttgatgatg caaaatcaaa aggtaaatat ggattagttg tgctttcgtc
      421 tagtaaaaaa atgccttttt tatctgatgc taaatatcta aaatataagg acttttgttt
      481 agcagataca tcagaaccaa attatgagct attatatttt ccttttactg ataatgctcc
      541 tattccatat tttaagaatt gtgtaaaaaa aagaaaatta aatgagaacg gtatagtttt
      601 atattacagt aatcagtgtc cacatacgga aaaatatgct cctattttga aaaaaattgc
      661 aaattctaga ggtattgatg taaatcttat aaaattcgaa acagcaaaag aagcacaaaa
      721 tgctcctgca ccatttacta catacagtct tttttttaat ggtgaatttg taaccaacga
      781 gatactttct gagaaaaaga ttgagaaatt tttagatacc aacattaaaa aatttaacta
      841 aaaagagatg taaatattgt attttgaaaa tatattattt tataatttaa tatattttgg
      901 atgtttttta aattgacgaa tgaaatataa aaattatatc gcaatttaga tacaaaatgc
      961 tggaaattaa ccatatagaa aatatttcca aaaatttaaa aattatattg acaaagttaa
     1021 aaaaataaac tatcatataa aacaataaga aaataaattg aaatcctgtg aaaaagagag
     1081 tagcataaag ctagttgata gagagctggg aatggtggaa gcctagtaac gaattttatt
     1141 gtgaatagag ctttggagga gattaccaga agtaatagta tggtaattgg tgaatctcgc
     1201 cttaagagat tagagtgggt aagattatct tatcaattag agtggtaacg cgggtgtata
     1261 ttcgtctctt ggtctttgac caagaggctt ttttaatttt aaatgtacat tttgtagata
     1321 ctttttaatt tgagttttaa tgattattgt aattttacca ataagagtgg taccgcggaa
     1381 aatcgcctct tagtatttat aatgctaagg ggttttttgt tttaattaga gtattttaat
     1441 ctacgtatta tcaaatgatt atcatatagt aaagtggaaa gataggattt taagactatt
     1501 tgaatataaa aatggaggga acaaaatgaa taaaataaaa gttggtatta ttggtgcaac
     1561 tggatatgtc ggggcagagt tgattagatt attaatgaat catgataaag tcgaagtgac
     1621 agcgataggt tcaaattcgt atgttggaaa agatatagtt gatatatatc caagtatagg
     1681 atataaaaat aacatgatat gtatagaaaa tgaaaaagtt atagatatgt gtgatgttgt
     1741 atttacagcc cttccacatg gagttagtga aaagtttgta ataaaggcaa ttaaatcgaa
     1801 aaagaaagtt atagatttag gtgctgactt tagaataaaa gatgaagaag tatatagtaa
     1861 atggtatgga gtatctttta tagataaaat cttacataaa aaagctgtat atggattaag
     1921 tgaaatatac aaagaagata taaaagatgc agatataatt gcaaatcctg gatgttatcc
     1981 aactagtatt tctttaccat tgatgccatt actaagttct aaattgataa aaaataataa
     2041 tataataatt gattctaaat caggtcttac aggtgctggt agagaattat cgattagtag
     2101 tcactttacg gaggttaatg aaaatatcac tgcatataaa atagggaagc acagacatac
     2161 tccagaaata gaacaaaatt taagtgaatc ctgcaaagaa aaagtaagtg tagtttttac
     2221 accaaactta atcccagtaa ataggggaat cttatcaact atatattgta caaaagaaga
     2281 taatatttct ataaatgata tacataggaa attaactgat tattatgaat ttaaagaatt
     2341 tatagaaatt cttccattag ataaggtagc ttctttaaag aatgttagat tctcaaataa
     2401 atgtgtaata tcattacatg aaaatggaga tacattgata atctgttcag ctatagataa
     2461 tatgattaag ggagcagcag gtcaagctat acaaaacatg aatataatat ttggtattga
     2521 agaaaataca ggtttaaaaa atattgcacc atcattttag aaataaaaaa tactaaactc
     2581 atataattag gaggataaat atggagatta taaagggtgg agttacagtt tcagaaggat
     2641 tttttgcttc agggattcac tgtggcttga gaaaaaataa agaaaaaaga gatttagctt
     2701 tggtttattc agatgtttta tgtgatgcag cagcagtagt aactcaaaat aaagtaaagg
     2761 gcaatcctgt ttatgttaca caggaacaca ttaaaaatgg aaaagcacaa gctatcatag
     2821 taaatagtgc taatgctaat acctttaatg gaaaagaagg acttataaat gcttataaaa
     2881 tggctaaatt caccagtgat aaattaaata ttaaagaaag tgatgttttg gttgcatcta
     2941 caggtgttat aggaaagcca ttaaatatag atttaataga agaaaatatg gatgaattag
     3001 tgaataatct aagtaaacaa ggtcatattg gagcaagaga agctattatg acaactgata
     3061 ttataaaaaa agaaatagca gtagcaataa tgtatggaga taaaaaaata actattggag
     3121 gtatggcaaa aggttctggc atgatacatc ctaatatggc aacaacgtta ggatttataa
     3181 ctacagatgc aaatattgat ggagttttgc taaaagaagc tttaaaaatt gctgttgaca
     3241 aaagttttaa tagagttagt gtagatggag atacaagtac taatgatatg gtaatgattc
     3301 tagcaaatgg aaaagcaaaa aatgatagaa taaacaaaaa agatgaacat tatcaagtat
     3361 ttttgagtgc acttacatat gtctgtatag aacttgctaa acttgttgca aaagatggag
     3421 aaggatgtac aaaactaatt gaatgctata taaatggagc attaagtgaa gaacatgcag
     3481 taaaattagc aaaaacagtt atatcttctt cattagtaaa gacagctgta tttggagctg
     3541 acgcaaattg gggtagaata ctgtgtgctt taggttatgc aggtgaagaa atagatatgg
     3601 aaaaagttga tattattttt gagagtatga aaggatatat tgaagtatgt aaaaatggaa
     3661 atggattaga ttttaatgag gaaaaagcaa aaaaaatcct tgaagatgac gaaataagta
     3721 tattagtaga tttaaatatg ggcaatgcca gaggaaatgc atggggatgt gatttaagct
     3781 atgattatgt aagaataaat ggaagttata gaaattagga ggatatactt taatgattaa
     3841 tatagaaaaa gcaaatacac ttatagaagc tttaccatat atagaaaaac atcagggaaa
     3901 gactattgta gttaagtatg gtggaagtgc aatgaaaaaa gatggtctaa aagaatctgt
     3961 tatggaagac cttgtgttaa tgagttatgt tggaatcaat atagttttag tacatggtgg
     4021 aggggcagaa atcaataaga tgctagcaaa agtggatata gaaagtaaat ttgtaaatgg
     4081 tcttagatat acagatgaag agaccatgga aattgtaaaa atggtacttg ctggaaaggt
     4141 taataaagac ttagtaaata aaatacatac aaaaggtgga aaagcagtag ggctttgtgg
     4201 aatagataat aatatgattt tatgtgaccc atataaaaat tatgaacttg gatttgtagg
     4261 agaaatcaaa aaagtaaatg ttgagcttat agaatcttgt ctaaagtcag gttatatttc
     4321 agtaattgca actataggtg ttggagatga tggagagact tataatataa atggtgacac
     4381 agcagcttct gctatagcta aagagttaaa tgcagataaa ttgatacttc ttacagatgt
     4441 ccctggtttg ttaagagagc cagatgaaga aaaatcatta ataacagaag ttatattaga
     4501 ggatgttgat aaattatttg aagaaggtat aattactgga ggtatgatac ctaaaataga
     4561 gggatgcgta gatgccttaa ataacggagt aaatagggta catattttgg atggaagagt
     4621 tccacattct ataataacag agctatttac tgatagtgga ataggtacat taataagaaa
     4681 agaaactgaa taaaattata agtttaagag gggagaataa gaatgaataa cgacaataca
     4741 atttcaaaat ggaatgaata ttttatagat acatataacc aacccaattt tgtcatagat
     4801 tatggtgaag gaagttgttt ttttgatact aatggcaata aatatataga ttttacaagt
     4861 ggatatggag tatcttctct tgggtatagt aatagtaatt taaagaatgc tctaaaagag
     4921 caagtagata aattattaca tacttcaaat ttatatttta atgagccagt gttgtattca
     4981 ggagagaaaa taattaattc aagtggaatg gctaaagtat atttttgtaa ttctggaaca
     5041 gaagcaaatg aaacagcatt caaaatagca agaaagtata gtagtgacaa atatggtaat
     5101 ggtagaggaa ctatcataag tttaaaagac tcttttcatg gtagaacaat gatgtcttta
     5161 atggcaactg gtatggacaa ataccataag tatttttatc ctcttcccga aggttttaaa
     5221 tatatagaaa gaaataatat tgaagattta aaaaataatc ttgattccac agtttgtgca
     5281 ataattcttg aagcgattca aggcgaaggt ggagttaatg ttcttgaaaa agattatgtt
     5341 ttggaaatag taaaaatatg tcaagaaaag gatattgtag tgatttttga tgaagtccaa
     5401 tgcggtatag gaagaactgg taaacttttt ggctatgaat attttgatgt aaaaccagat
     5461 atagttaccg tagcaaaagg gttgggagca ggaataccag taggaggagt attggtaaat
     5521 aaaaaacttt caaaagtact aggaaaagga gaccaaggta caacttttgg aggtaattta
     5581 ttagctatgg tggcagcttc agttgtatta gatgagatat ctaaagatgg cttttataat
     5641 gaagtattag aaaaaggaaa ttatataaga aaatctattg aaagttttaa taacaaagtt
     5701 atattaaaga caaaaggtat tggtctaatg ataggaattg aaactaatat tgaatctagt
     5761 attatagaag agaaagctag aaaaaaagga ttgcttattt tgactgctgg aaaaaatgta
     5821 ttgagatttt taccaccatt aacaatcagt tataaggaaa tagatgaggc tcttgaaatc
     5881 ctaaaagata tattactaga aattaactaa atattcaaaa aatagttgat taaagataaa
     5941 aattagaagt aaataaaaaa gtaaagtgct aacaagttta attgaattta aaaagtttat
     6001 aagaaggggc gaattaatat gaataatata agtttaaaag gtaagagttt tttgacatta
     6061 aaggatttta caaaagaaga gattagatat ttattaactt tatcaagtta tttaaaaact
     6121 aagaaaaaag ttggtataaa aggcgatttg ctacaaggaa aaaatatagc tttattattt
     6181 gagaaaacat caacaagaac tagatgttca tttgaagtag cagctcatga tgaaggagca
     6241 catgtaactt atctaggtcc aaatgattgt catatgggta aaaaagagtc tgtatcagat
     6301 acagcaaaag ttctaggaag attctatgat ggaatagaat ttagaggctt taaacaagaa
     6361 acagttgaaa gtctagcaaa atattctggt gtacctgttt ggaatggact tacagatgaa
     6421 tatcatccaa cacaaatact ggcagatttt ttaacagtaa ttgaaaatgt agataaagat
     6481 ttgaataaag ttaaatttgt atatgttggt gatgctagaa ataatatggg gaactcactg
     6541 atgataggat gtgctaaaat gggaatacat ttcgtagcac tagcacctaa ggaactttgg
     6601 ccaaatgaag aattggttaa ggaaatgaag gaaatagcca agaaatccaa aggagatata
     6661 atgttgactg aaaatattga tgatgttaaa ggtgcagatg ttatatatac agatgtatgg
     6721 gtttctatgg gagaagagga gcaatatgaa gcaagaataa atcaacttaa agattatcaa
     6781 gtaaatatgg atatgataaa gaaaactgaa aatgaaaatg ttatattttt acactgtctt
     6841 ccagcatttc atgatttaga aactaaggtt gctcaggaga tttatgaaaa gttcggatta
     6901 tcagaattag aggttacaaa cgaggtattt aattcaaaat attcaaaggt atttgaagaa
     6961 gctgaaaata gaatgcatac aataaaagct gttatggttt ctactatgac tgatgtaaat
     7021 atgaacaata atttaatata gatatgtttt aatatagact ttactttaat gaaaatttat
     7081 aaaatatttt aaatatatta ttcaaaaact ctcattatat gttaaaataa cttatgtatc
     7141 aacaaattaa gagggaggat ttgagatatg aaaaaaatca tattagcttt aacaactttt
     7201 ttattagcaa tatctttaac agcatgttca agtgcaaaac cagaagatac aattgatagt
     7261 ttttttaaca gtgcgaaaaa gtttgacttt gaaggcatga ataaggtaat ggaaaataat
     7321 gacgaaaaat ataaggatat attaaaggaa ttagatacaa aagacccaaa tgcccaatat
     7381 gttttagatt atctaaaaca gaatgcatct aaaatcacat atacaataaa agacagcgag
     7441 gttaaaggtg ataaggctac aatcaaagta gaatgtaaat ttatagattc aacaccatta
     7501 cttcaagaaa tagtagctga ggcatttaca aagatgattg gtatgagttt ttcaggacaa
     7561 gacttaactg atgagaaaac aactgaaatg ttagtatcaa ttatgaaaga aaaacaaaaa
     7621 agcgttaaag agacttatgt tacaaaaaat gtagagtttg aatgttctaa aaaggataat
     7681 aaatggataa taagttcagt aaatgatgca gtagctgatg tattgttatc taatcttgtt
     7741 acagcaggac aagaattttc taactctatg aatacacttg aataatattg tttaggtgta
     7801 gtaaagtaat aaatatatga ttatttttat aaataaacat ataaatattt atgaaggata
     7861 ccatttttgg tatccttttt attaatataa tgaaatacat ggttaatttc tatattgtca
     7921 agaaactatg atatatgtta taagtctata ctatttaaaa taaacttata acatatattt
     7981 tttataatat agtgtatgaa aaatttattt aactaatttt agatatttat tgacaactga
     8041 ttcagccaag cattttgatg aatcaacaat atttattgaa ggatatgatt tttctaaaac
     8101 tacatttaaa tcagtacaag caattactat attttctata gattcatttt taacatcttc
     8161 aataagttta ttccaaatat caatattgtg tggattttct ttatccattt ttatattttg
     8221 aataagattg tttaatttta tttgccattc atctttaaat ataaattcat gtccattatg
     8281 tattatacca ttctggtaaa ttgtagactc aaaagttgaa ctagtcgaaa aaagagttac
     8341 tttttgtgag atagtaggta attttttaac tgtttcttct acaatattta atattggtat
     8401 attaatagac tcttttaatt ctttaaagta tatatgtgct gaattgcatg gcattgcaat
     8461 aaaatttaca ccagttgatt caagcttttg taatccatca attatagttt ctttcataag
     8521 gtcatgattt attggacggt caatataaaa tggggtaggt aatgaatata tcatcatctt
     8581 tggaaattca tcatcatatt ttgctccata ttgagattga cactcatcaa taattaaatc
     8641 aacaaaaggg gctgttgacc ttggacccat tccagccaaa ataccaatta atttttcatt
     8701 cataaaaatt atctccttta tatataaata tatttatgta taaaataaaa atctataaaa
     8761 gcataaaatt atattttata atagattata ttgtacactt atataatata aatataaagt
     8821 gattttttag attatatatt ttaatatact ttcttatata agaattattt taaatcaaga
     8881 ttatctagta tttttaaaaa tatttttaca gcagtatcca agtcatctac agagacactt
     8941 tcattaggat tatgacttac gcccccagcg catcttataa ataccatacc aatatcagtt
     9001 atattatcca tttcctgagc atcatggcca gcaccactat aaatataaaa aggattaaga
     9061 tttaaatcta taaaactttt ttctatgatt tttgttattt tattagaaca tggtacagga
     9121 acattttcaa aagcaagttc acttgtataa ttaagttttc tattttcaca tatatgtgat
     9181 atttcattaa atatcttttc tacagaatta tcaagaattt cttgtgaaca agagcgtaca
     9241 tcgagtgtaa attcagctct ttcaggtacc acatttgatg aagatggata gaaattcatc
     9301 tttcctacag tagcaaccaa atcagtagtc gttttagcta ctttttcgat agctaataca
     9361 cattcacagg cacaacatcc agcatcaagt ctcatattca ttggaagtgt accagcatgt
     9421 ccagacttac catttactga tatcttaaaa cttttaaatc ctgtaatact tgatacaatt
     9481 ccagcaggaa gagattcatt ttctaaaaca ggaccctgct caatatgaaa ttctaaatat
     9541 gcatctacat cttttctagg atgcaaattt gttaagtttt ttgtattaaa tccaaacttt
     9601 gttacagctt cttcaagtgt aattccattt tcatcaacag atttaagaag ttcctcttta
     9661 aaagtaccag caaaaacttt acttcccatt aaagaagttt caaatctagt tccttcctct
     9721 tcagcgaaag caattagctt aatattgaaa ggataagacc tattattgtg ttttgcttct
     9781 tcaagaccaa ctagaggcac tattatacct aacataccat caaatatacc tccacacttt
     9841 acactatctt gatgagatgc aactactaat gtgggagcat ttggaaagct tgatttatat
     9901 gtacctataa cgtttccaat gtcatcaatt tcaatatcaa gatttaaacc ttccatccat
     9961 gaactaatta aatcaataga ttttttatgt tcttgagtca agataagtct tgtaagacca
    10021 tttgttgttg aacttatttt tcctaatgtt tgtagataat ccatacattt ttttcctaac
    10081 atatttacct cctaaaaata taatttttat ttaagctaag attagctttt atacttataa
    10141 tttttgaact ttcaaataat tttgtatgta tagtaaaata ctattttagt attaataatg
    10201 atataaaaac ataaagtctg tagacgaaga gcagaagttt taatttaaaa atatgaaata
    10261 tgatttttat tattatagta tttttctata ataatatctc tttccagtta gaggataatt
    10321 ttcaagtgaa aatactttat ggtatccaaa tttctcataa aatttaggtg cttgaaaact
    10381 gaatgtatct aaaaaaacat atttacaacc tcgttcttta gcaatttttt cagccttata
    10441 taaaagttga cttccagtac ctttatatct aagcttctca ctaacccata agtattttat
    10501 agaaagccaa ttaccatgag taacacctat aaaacctgca acaattttac ctgctttatc
    10561 tcgaatatat attcctaaat ccactgggtt tttatcttca agtctagcaa gattatattt
    10621 taataatcct tgaaaaatag tatcttcatc ttgtttttct attttttctg taatttctaa
    10681 ttccatattt gaactttctc cttgtataat cttttgtata ttggtatttt ttaataaaat
    10741 cagtagaaag aatatagttt aacattattc taataggcat ttatgctaat gtc
//
LOCUS       DAMJYX010000107        10703 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJYX010000107 DAMJYX010000000
VERSION     DAMJYX010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.111"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            98..1297
                     /locus_tag="PC479_002954"
     CDS             98..1297
                     /locus_tag="PC479_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2805479.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1349..1852
                     /locus_tag="PC479_002955"
     CDS             1349..1852
                     /locus_tag="PC479_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805480.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            1959..2276
                     /locus_tag="PC479_002956"
     CDS             1959..2276
                     /locus_tag="PC479_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805481.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2434..3393
                     /locus_tag="PC479_002957"
     CDS             2434..3393
                     /locus_tag="PC479_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2805482.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3508..4293
                     /locus_tag="PC479_002958"
     CDS             3508..4293
                     /locus_tag="PC479_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805483.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            4585..5904
                     /locus_tag="PC479_002959"
     CDS             4585..5904
                     /locus_tag="PC479_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2805484.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(5979..6407)
                     /locus_tag="PC479_002960"
     CDS             complement(5979..6407)
                     /locus_tag="PC479_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805485.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6498..7874)
                     /gene="hydG"
                     /locus_tag="PC479_002961"
     CDS             complement(6498..7874)
                     /gene="hydG"
                     /locus_tag="PC479_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2805486.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(7875..8960)
                     /gene="hydE"
                     /locus_tag="PC479_002962"
     CDS             complement(7875..8960)
                     /gene="hydE"
                     /locus_tag="PC479_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2805487.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(8996..10204)
                     /gene="hydF"
                     /locus_tag="PC479_002963"
     CDS             complement(8996..10204)
                     /gene="hydF"
                     /locus_tag="PC479_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2805488.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(10219..10470)
                     /locus_tag="PC479_002964"
     CDS             complement(10219..10470)
                     /locus_tag="PC479_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2805489.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT         3950 a         1297 c         1663 g         3793 t
ORIGIN      
        1 tgagttctat tttaatacaa ttaaattaag cttagtttga aatttgtatc taaaaagtaa
       61 agtaatctag aattaagaaa atggagatag aattataatg gaaaaattat attatacaaa
      121 tcagtatata agagatttta cagcagaaat aattgaaata aaagaagtag aaaataagta
      181 tcatgtttta ttagataaga cagctttttt tcctggtgga ggaggtcagt ttggagacct
      241 gggaaaaata gagaatgatg atgtaataaa tgtgtatgaa gatggaaaaa aaatatacca
      301 tgtattagat aaaaaaccta taaagataca taaggtaaag tgtagtattg attgggatag
      361 aagagaagat ggaatgcatc aacattttgg acaacatgta ttgtctggat gtttttttaa
      421 aacttttaat gcaaatacga caggttttca tttgggaaat gagtatagta cagtagatat
      481 agaaggtaat ttagaagagg ataaaattag agagatagag ttgtttgcca atgaaattat
      541 tcgtcaaaat atacaagtag agacattact tccaacgaaa aaagaactaa aaaagatttg
      601 gttaagacgt gatttgccaa atactgatga agagataaga gtaataaaaa taggtgattt
      661 agatacgaat gcatgttgtg gagtgcatcc aaaatcaact ggtgaattga gaatgataaa
      721 aataaagaaa tgggaaaaac ataagtcaag tacaagaata gagtttctag caggtaaaag
      781 ggcagtggat gaagtattga agagggatat atatttgact aaaatatgta gatatttaag
      841 ctgtagtgag gaagatgcta taaatggtat tgtaaatcta aatagtaaag tggaagaggc
      901 tttaattaaa aaaagaaggc ttgaagaagt agtatctaca tatcaggtca aagaaatgat
      961 tgaaaaagca aataaggttg gcaatatttc ggtcattaaa aaaatttatg atgatgaaga
     1021 tttgaaatat gtaaataaga tagcaaataa aattgcagaa tatgaaaata gtattgcatt
     1081 attagctgtt aaatttaatg ataaaataaa tttagttttt gcatgttcta aaaatttaaa
     1141 agatattaat atgggagttt tattaaagga tgcaataaat cttatagatg gcaagggtgg
     1201 tggaagtaaa gtattagctc aaggaggagg taagaataat ggaaatttag aggctacact
     1261 tgattatgca tttatgaaga tagaaaaaac tctataaata tattatataa aaaaccttag
     1321 tagataaaat agatatggag gaataagtat ggagcaacaa aaggactaca tagagttata
     1381 tgaaaatgaa gaactaatag agataagaga agcacgtatg gatgatttgg atacaatagc
     1441 aaaatttaat tataatttgg ctaaagaaac tgaaggaaaa gaacttgata tggatgtttt
     1501 aactaaaggt gtaaaagcat tgttattaga tgagagaaaa ggaaaatatc atgtatatac
     1561 agtatttgat aaagttgttg cacagattat gtatacatat gagtggagtg attggagaaa
     1621 tggaaatttc ctttggattc agagtgtata tgtagataag gaatatagaa gaaaaggtat
     1681 ctttaattat ttatttaatt atataaaaaa tatatgtgat aaagatgaaa atatagttgg
     1741 aatgagatta tatgtagaaa aagaaaacat aaatgcaaaa gcaacatatg agtctttaaa
     1801 tatgtatgag tgtgattata atatgtatga atacgaagta gtacattcgt aatataaatt
     1861 tgtaatactg ttatactgat gttataaagt attgattata ttgacgatta tatgaaataa
     1921 actggatatt ataattagaa tttttagggg gtataaaaat gggcgtttta aaaggaatgt
     1981 tttatgtatt tttattttta gatttagtgt caatattctg tttctttttc aataaaggaa
     2041 aaatagcatc taataaaatt gtgtttaatg caataggagt acttacattt gtactttgtt
     2101 ttatgctttt tagttattat cctaataata atcttattgg aaaatttatt gcttctctat
     2161 tttttatatt tggagttgca ggagttatgt tgaaagaaaa aaatttttta tatgctagac
     2221 ttcttttaac tgtagttata gttttttcta cattaagatt gttcgttatt caataaaatg
     2281 tatttgtata aaaatttatt aaagtctaca catttaagtg taggcttttt tgattttttg
     2341 tttgaaaatt aaggaaattt taaggaaatt ttaaaatcgt cttaaatatt tattgatata
     2401 cttaaaatat taatagcgaa aggagactta tcagtgatag aaagtaaaaa gtcagaaaaa
     2461 ttgttactgt caattatata cataatattt ttggtatata ttttaatgat tataagaata
     2521 attttattta gagatgtacc aatttacgct attttcaagg gaacgtttag gaatgtaaat
     2581 ttaattccat tttatacaat atatcaattt atagtagata gtaatcttga ctttatgaaa
     2641 gctactgtaa atattataga aaatataggt atatttattc ctatgggaat ttttttacct
     2701 atagtatgta aaaatctaaa taaaaaaact ataattatag taattatact tgttagccta
     2761 gcatttgaat taacacaata tatttttgct cttggaagta gtgatataga tgatgttatt
     2821 ttaaattcat taggtggcat aattggaatt aatatttaca taaacatgaa taaattattt
     2881 cccaatgata ttaacagatt taaggctata ataggtacta gtttagtttt aggagttata
     2941 ggattaggag ttatttctaa aaattatcat aacttactaa cttttaaatt tagacctgat
     3001 agaaaaatat caaaaatatt aattgaagaa aatagggaaa taattaaaga tataaataaa
     3061 gatacagtag atatagttgg gacatttgag agctttaaaa aaggaataat aactattaaa
     3121 gcaggttcta ataacaaagt aaagagacca gagaatttaa ttgattctga tggaaatata
     3181 agaatttatt taaatgaaaa tacaaaatta gtatcttata taattaccaa agaatcaaaa
     3241 atggatatag ttaaatatga ggaatttgat gtgaagaatt tagatttatt aaggagatat
     3301 gatactatta atgtttggat tgataaagaa aatcagccaa aggataaaca tggtattatg
     3361 gctagtaaat tattaattgg attgtatgaa taaaatatta aacaagaata aaagaataac
     3421 aaatataaaa aacgaaaaaa ctatttctat aattattttt acgttgtgca taaaataaga
     3481 acattagtga aggagtagag ataaattatg gagataataa aatatatctt gtactttttt
     3541 atatattcat ttttaggatg gacagtagaa agtataggat gttctatagc aagcaaaaga
     3601 ataatcaaca gaggtttttt aaatggtcca atatgtcctg tttatggttt tggagcggta
     3661 attgtgatat cactattggg aagatttaat aatgtagtaa tagtatttct attaggaatg
     3721 attttaacta cgatactaga atattttaca ggatttattt tagaaacctt atttcatgct
     3781 aaatggtggg attattctga cagaaaattc aatataaagg gtagagtatg tttaaaaaat
     3841 gcaatatact ttggtgtaat gtctgtttta ataataagat ttatacatcc atttataaaa
     3901 tattttgtaa gtataattcc atatagaata ttaatttcaa tggctataat tacaacactt
     3961 tggactatac ttgatttgat tgttactatt ataacactta aaaaattaga tataaagtta
     4021 aacttactag acgatataat tacagacctt aatgatataa atgtgaaatt ggataaattt
     4081 gatagaggag aaatacaagc tttatttaaa acaataaata acgacgaatt agaagttaga
     4141 gaaaaaataa acaaaataaa tagtaagttg gaccgtatag agtctaatat tattttacaa
     4201 aaaagagtta taaaagcttt tccacatata aaacataaaa aacaacaaga gcaactagaa
     4261 cattttaaaa aaattattaa tgagaaaagg taattataca atttaatgaa gacgaataga
     4321 ataatggtat ttataagatg attttcagag gactctataa aaatagaata gagtccttta
     4381 ttttttgaaa aataatgttt atttgataaa aataaaatat attgtttctg aataacaaat
     4441 attttgaaaa ttataaaaac atatattgac aatttgtgtc aaaaatacta acattaagta
     4501 tataaagaat tttatatgag aatatcttta taaatcgcaa taattttaaa acataaattt
     4561 atttaaagag gggatgattt gccaatgagc gagataacta gtcaaatgat aagtacagaa
     4621 gaaaaaaaat atgttgctaa aactcaaaag ataccttatt atccagtagc atttaaatca
     4681 ggtgatggtg caatgctata tgattatgaa ggtaatgaat atgtagattt tttagcaagt
     4741 gcaggctctg caaatgtagg tcatggtaat aaggaaatct cacaagcagt taaagaacaa
     4801 atggatgata taacacaata tacacttgca tatttccata gtgatcctcc tgtaaaatta
     4861 gctgagaagc ttgtggaaat agcccctgga gataatgata aaaaagtact atacagtgca
     4921 actggttctg cttgtataga tgctgctata aaattagcaa gaggatatac tggaagaaca
     4981 aagataatat ctatgtgtga atcttatcat ggaagtactt atggagctat atctatatct
     5041 gctttaagta caaatatgag aagaaaaatg ggtccattgt taccagaagt ttatcacttc
     5101 cattatcctg ataaaaatag aacagctaaa gaatgtttag atgagataga atatgctttt
     5161 gcacactatc taccagcaga agaggtagct gcaatattta tagagccaat tgcaggagat
     5221 gcaggaataa tagtgcctcc agtagagtgg gtacaaggtt taagtaaaat atgtaaagaa
     5281 aatggaatat tacttgtaag tgatgaaata caacaaggta tgggaagaac aggtaagtgg
     5341 tttggtatag aaaattttgg tgttgaagca gaccttatag ttcttggaaa atctgttgga
     5401 ggtggattac ctctaggagc agttgttgga agaacagaaa taatgcaaag tcttgatgca
     5461 ccagcacatt tatttacttt agctggaaac acaacagtat gtgtggctgc tttaaaatct
     5521 atagagataa ttgaaaaaga aaacttactt caaaagagta tagaaatggg agattatatt
     5581 aaagcaggat ttgaaaaact aaaagaaaaa tacgatataa ttggagaaat aagaggcata
     5641 ggattatcta taggagttga tatagttaaa ggaaaaggtt ctaatgaaaa acatccagat
     5701 gctacagcta agatatgtta tagatgtata caaactggat taataatgat attcttagga
     5761 caatctactt taagagtgca accacctcta gtaataacta aggagcaagt agataaagca
     5821 atgaacatca tagattctgc aatagatgat tatttaaatg gtagaattgg tgatgaagta
     5881 tatgaagtaa ctcaaggttg gtaaaataaa tattaaatga aaataggagt tatctcaatt
     5941 ttagaaaatt atttctgaaa tgagataact cttttaagtt acccacaaat aagaaaattg
     6001 tttgattcat taaagactat atttcggatg tcgtttttgt gagtatcaga gacagtatta
     6061 cgagaataat tacctttatt cgtactatct gttaatttag ttataattaa gatagctaaa
     6121 agaataagaa ttactgtgta taaagcaata acatatggtt ttcttttata tttgtatttg
     6181 ttattgaaaa aagaatggcc ataattattt ttatttactt tagatgtatt tgtattcttt
     6241 ttcttgttac ctttcttttt atcatccttt tttctatttt tagggtaagg aatgactttg
     6301 tgtgtattat caagtttgat atttttattt ttttgaatat tgttttccag gctagccttt
     6361 ttttctccaa attgaatagt tttattggta ttgttgttat ctttcaatgt taatctccct
     6421 cccttaaact ttccctttag ataatattac tactaaccta tggaatatac aataaataat
     6481 atataagtaa taaaatttta taggtataaa tctctttctc cacgctctaa ggcagtcata
     6541 ttgttagata ctatgtttct tatatcttct ctttctatct tacctatttc ttctgcgata
     6601 agttttttac ccataatttt aagttcttca tctccataat cgattaaaaa ttcatttaat
     6661 gtagtaagag catttggtgt acatacatta tggatttgtc gacttttagc taagctcatg
     6721 aatctatcgc cagttctacc tttacgataa caagctgtac aataactagg tatatatcca
     6781 tcagtaatca gttctttaag cacctcaaca gggcttctat ggtctccaac ttcaaattgt
     6841 tctgtattgt caccatcttc atatgcttta tatcctccaa cacctgttag agaacctgca
     6901 ctaatttgtg atacaccata ttttagtaac tcatttctca tttcagcagt ttctctagtg
     6961 gacattatga ttccagtaaa aggaacagcc aatctagtta tagctactat ttttttgaac
     7021 atatcgtcag aaactaagtg aggaaaatct tctaagctca taccttcagc ttttttcaat
     7081 ctagggaaag atatagtatg gaaacctaca ccaaattttt cctctaagtg ttcgttgtgc
     7141 atcataagtc ctagaacttc aaatttaggg tcagataaac caaataaaac tcctgcacca
     7201 acatcatcaa caccagcttc catagctcta tcaaaagctg ttaaatggta ataatagtca
     7261 ttttttatag attgaccatg catttttata aatgttggtt tgtgataagt ttcttggaat
     7321 aatatataag ttcctattcc tttttctttt agtaatttat actcatctac acttgtagct
     7381 gctatgttaa cgtttaccct tcttatattt ccgttttcat tatatgttga ataaattgca
     7441 tctaagcaat ctaagatgta atttatatca caatttttag ggtcttcacc agcttctaga
     7501 gcaagtcttt tatgacccat tttttctaat attttaacct cttcctttat ctcttccatg
     7561 gtaagttttt ttcttttaaa tttgttgcat ttactaaaac cgcaataaac acattcattt
     7621 acacagtagt ttgatacata aagaggggca aataaaacca ctctattacc atatatttca
     7681 ttttttattt gcccagctat actaaataat ctatctagtt gttttttatc ttctacttca
     7741 agaagtgtag ctatatcttt ataactaagt ttttcccttc tatcagcctt atcaagtacc
     7801 ttttcaatat catcagaagt tgaatttttt gcatcttcta ataaagaatt aataagttca
     7861 tggtttatga acatttacaa acgcctccat tcaatattat caccgcgaga atagtcaact
     7921 tcaagtccta atgacttgat gttttcttct aaactttggt gatattcaca agcttcttga
     7981 ccagtaaagg ctttgttatc gtaaagagaa tattgttttc taaattcttg aggagaaagg
     8041 tttggcataa ttacattgca accagctaaa agacctgcat ttctaccact agggtttata
     8101 gatgccaagg ctgttgtagc gggtaataaa acttttggaa gtagaagtct tgtaattgaa
     8161 agcataagta atgtcttctc taaatctcca tgtttataat ccttaaaaat tgtatcatgg
     8221 tgtggaataa aaggtcctat tccaaccata tgaggattta actcttttag gtataataaa
     8281 tcacgaacaa ggtcttcgtt gttctgaaat ggtgattcaa ccataaatcc agcacctacc
     8341 tgataaccaa tttctttaag gttttttagg cattcttttc tagttctaag ctcaatgttt
     8401 ggcggatgta attttttata atgactgtct gtagcagttt catgtcttaa aagatatcta
     8461 tctgcaccac ataaaaagta ttttttatac gaatcataac tcttttctcc tagtgataga
     8521 gttatggcac aatcctcata tttatttttt atacttgaaa caatttcaca tattttatca
     8581 tcagtaaaat atgcatcttc accaccttgc aaaacgaatg ttctgtatcc tattttataa
     8641 cctatatcac aacattcaag tatttcatca agtgaaagcc tgtatcttat gatattttta
     8701 ttagaagatt gtattccaca atagtagcaa ttatttttac aatagtttgt aagctctata
     8761 agtcctctca agtagactct atttccataa tgtttatttc tcatgtcgct agcttgttta
     8821 taaagatagt ttcttaaatt ttgttcgaag ttatattcct cattgtcgat attttctatt
     8881 aaatatagta gttcatcaaa acttaatgaa ttaatgctgt aaagtttatc tataaagtat
     8941 ttaatcttat ttttagtcat attcaaatat gtaattatta agtatagtta aatttttaat
     9001 aattgaaaag ttcaagtgat ctttccaaaa taccttttac atatgctatt aagacaccat
     9061 agtttacaat aggtacattg aaagattttg ccttttctat tctagataac attccagctc
     9121 tattcatcat acaagctcca cagtgaacta caagggcata tttatttata tcttcagtga
     9181 aggatactcc agaactaaat tcaaaagtaa tgtttttgcc agttttcttt ctaatcatat
     9241 taggaatttt tactgttcca atatcatcag tttgtctatg atgggtacat ccttcggcca
     9301 tcaagatttt atcaccatct tttaaatttt ctaaagcata agctccatta atcaattctt
     9361 taaggtctcc cttttgtcta gcaaacaata tagaaaaaga tgtaagagga atatcttttt
     9421 gtgtatcttt atctacttgt ggaaatactt gagagtcagt aataacaagt tttggttttt
     9481 tgcctaaatt agatagagtt tcttttaaac tatcttcttt tgtaactata gaaattgcac
     9541 cattatccaa aatatctctt ataacttgct gttgaggaag tataagtcta ccttttggag
     9601 ctgctttatc tattggaaca actaaaacaa ctaaatcatt tggttcaatt aaatctgata
     9661 ccaatttgaa ctcagtgcta tccttaggca aaacttttat tatttcattt tttaaatttt
     9721 caattccaat tttatctgtt gatgagacag aaacaactgg acatttaact ttatttttag
     9781 ttaagtttaa aatttctgat tgattcttaa tagtatcaat tttgttaaga attagaatat
     9841 gagggatatt cttatcatta aatttttcaa ttagtgacaa atcttcttgt gatataccaa
     9901 tttggcaatc cactactagt aaagctatat ctgtcttttc taaaacatct agtgatttac
     9961 ctattctaag ttcaccaagt tcaccaacat catcaagacc agcagtatct ataagaacac
    10021 aaggtcctat aggaaggatt tccattggtc taaatactgg gtctgtagta gttccagcaa
    10081 tatctgagac tattgcagca ctttgatttg ttatagcatt tattatgcta gatttacctg
    10141 catttctttt accaaagaga cctatatgta ctcttaccga ttgtggagta gaatttagac
    10201 tcatttgtac tcaccacctt aaacttcttt ttttgatata ctagttttta cagatacatg
    10261 aggtatgttg cccaatttac cagtcaaact attaatagtg tccatttcgc caacaactgc
    10321 tatacatacg attgagacac cttcttcttc gcaaggaatg cccattcttc ctttaataat
    10381 acctttaaaa cctgatacaa cctcattgaa tttaaattga cattcttttg gttcttccaa
    10441 aatagcactt ataactgcaa cctttttcat aaatatcctc ctaactcaga aaatatgtct
    10501 taaacaaatc caaaatcaaa aaacttttct ttgtaaagaa aagtttctac aaaagaaaag
    10561 ttattaatta cacaagtata atgtgctata ttaaactatt tccttacaga ttagataact
    10621 cttttctggt agggttagct tgtaaaaatt taatttaaca acaagtaact gaagaaaatt
    10681 tttatacaat ttatataaat tat
//
LOCUS       DAMJYX010000108        10646 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJYX010000108 DAMJYX010000000
VERSION     DAMJYX010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.112"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..321
                     /locus_tag="PC479_002965"
     CDS             <1..321
                     /locus_tag="PC479_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2805490.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
     gene            454..1824
                     /gene="hydA"
                     /locus_tag="PC479_002966"
     CDS             454..1824
                     /gene="hydA"
                     /locus_tag="PC479_002966"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2805491.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1842..3248
                     /locus_tag="PC479_002967"
     CDS             1842..3248
                     /locus_tag="PC479_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805492.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            3320..3766
                     /locus_tag="PC479_002968"
     CDS             3320..3766
                     /locus_tag="PC479_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2805493.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3805..4599
                     /locus_tag="PC479_002969"
     CDS             3805..4599
                     /locus_tag="PC479_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2805494.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            4589..6880
                     /locus_tag="PC479_002970"
     CDS             4589..6880
                     /locus_tag="PC479_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2805495.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6881..8194
                     /locus_tag="PC479_002971"
     CDS             6881..8194
                     /locus_tag="PC479_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2805496.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8230..9315
                     /locus_tag="PC479_002972"
     CDS             8230..9315
                     /locus_tag="PC479_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2805497.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9545..10594
                     /locus_tag="PC479_002973"
     CDS             9545..10594
                     /locus_tag="PC479_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805498.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQ
                     EMIVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEI
                     NVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITV
                     MKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMK
                     EIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
BASE COUNT         4288 a         1117 c         2170 g         3071 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg acgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctatgcaggt
      181 gaagctggaa taaaaactat aggatttggt ccttctaagg aaaatcttgc tcacacaata
      241 gatgagtata tagagcaaga acagttattt ataggaacag aaggttacta tggaatatta
      301 aaatctgtat acggaaaata atatttagtt cacaataaat tatttaaata tagtctagag
      361 gtgctgaata tagcaccttt agaaaataca attttaaata aagttttaca atacttagaa
      421 aatactttaa agcttaaaag ttaggaggta tatatgggaa caatactaag aggtggaact
      481 ataattacct ctgataagac ttatataagt gatttaagga tagaagatga aaagatagtt
      541 gaaataggaa ataacttaga aataaatggt gataaggtta ttgatgctac aggtaaaatt
      601 gtaattcctg gtggaataga cacacatact cattttgata tgaatgcagg ttctattaca
      661 actgcagata attttgaaac aggaacaaga gcagctatag ctggaggaac tactacaata
      721 ctagattttg cagaagccaa tgagggggaa aatctactgc aaggtgtaga agcatatcat
      781 aaaaaagcaa gtggaaattg ttattgtgat tatggttttc atatgacaat tacatgttta
      841 aataaagata catttaatca tatggaaaaa cttatttcag atgggatagt atcttttaag
      901 atgtatatgg catatgatgg catgaaagta gatgatggaa caatatacag ggttttaaag
      961 aaagcaaggg atttaggttg tatagttgga tttcattgtg aaaatgggga tttactagat
     1021 gtattgatag aagaaaatat acaaaatggt aatttggagc caaaatatca tccacttaca
     1081 agacctaaca tagttgagaa agagtctgtt tcaagacttg ctgatataac taaattatct
     1141 aattcaaaat cctatgtagt ccacttaagt tgtagggagt cacttgaaac tgtaaaaata
     1201 gctagagaaa aaaatataga tatgatagta gaaacatgcc cacagtatct attattagaa
     1261 gacaatttat ataataagga taaatttgag ggtgctaagt atgtaatgtc tcctccttta
     1321 agaaaaaaag aagacataaa ttacctttgg gaaggattgt ctagtggaga tattcaaaca
     1381 gttggcacag accactgttc atttaatttt aaggggcaaa aagacttggg aattgatgat
     1441 ttcagtaaga taccaaatgg agcacctggt gttgaacata ggttagcact tttatataca
     1501 tatggggtct tagaaaatag aataagtgta aataagtttg tggaagttac ttctacgaat
     1561 gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga tagctgtagg aagtgatgca
     1621 gatatagtca ttttaaatat caataaagaa gaaacaatta gttataaaac tcaaaaacaa
     1681 aatgtagatt atactccata tgaaggattt aaggttaaat gtaaagttga agatgtattt
     1741 ttgagaggaa atcatgtagt acaaagttgt aatgttaaag aacatccaac aggtcaatat
     1801 attaaaagaa aaattactaa ataatgcggg gggaaataag aatgatggat atacttatta
     1861 agaatgccat aatagtaact gtgaataaag agagagaagt tatttttgat ggggcattag
     1921 tagtaaaaga taacaagatt gctgatattg gaaattctaa agagatagaa agtaaatata
     1981 ctgatgttaa aaaaattata gatgctaaag gtaaagtttt attcccaggt tttataaata
     2041 cacataatca tttattccaa actttattaa aaggattggg agatgatatg gtacttaaag
     2101 actggttaga aactatgaca tttccagcag ctaactattt agagcccaaa gatacttatg
     2161 atgctgcaat gcttggatgt attgaaggac ttagaagtgg tataacaaca atggttgact
     2221 acatgtatcc tcatagtaag ccaggtcttt gtgatggaat aatagatgct tataaagaat
     2281 taggaataag aggaatactt ggaagaggat gtatgaatac aggagctcaa tttggagttc
     2341 atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga tgtaagaaga ttgtttgaaa
     2401 aacaccataa tacagaaaat ggaagaatca aaataggagt agctcctgca gcaatatggt
     2461 caaattctca agaaatgctt gaaatgctat ggagagtagt aaaagaatat gatgatgctt
     2521 tatttacagt acatatatca gagactcctt ttgatagaga agcagcaaaa gaattgcatg
     2581 ggcaatatga catagatgtt ttagaaaaat taggaatact tggaccaaat gtgcttatgg
     2641 tacattgtgt gtatctaact gaaaaagata tggaacttac taaaaaatac gatatgaaag
     2701 tgtctcataa tacagcaagt aatatgtact tatcttcagg tgttgcacca gtgccagaaa
     2761 tgcttaaaaa aggaataaca gttagtttag gagtagatgg agccgctagt aataactctc
     2821 aagatatgct tgaacttatg aaattaacag cacttcaaca taaagttaat aagtgtgacc
     2881 cattagcaat gtcggcagaa aaagttcttg agttggcaac tatagatgga gcaagagcta
     2941 ttggaatgga agatgagata ggttcattgg agataggtaa aaaagcagac ttactaatat
     3001 ttaatccaat gctttcacca aaagcgatac ctatgcataa tccggtatct acattagtat
     3061 attcttctag tatgaaaaat atagagagtg ttatagtaga tggaaatata ataatggaag
     3121 attcaaaaat attgacagct aatgaagaga aagcattaaa agatgctcaa gatacagctg
     3181 agagactttg tataagagga actataaaaa atagaatgga aggtcataaa tggaatagcc
     3241 tttattaata aatgtaattt attcataaaa aagtatagta aaactttagc ttaaaaatat
     3301 gagttttgag gtgatttatt tgattatcaa atgtaccata aataataaag aaaaagaatt
     3361 aagtataaga ccagatgatt ttttagcaga tacattaaga gaaaatggat atgtaagtgt
     3421 taaaaaaggg tgtgatacag gctcatgtgg gttatgtaca gtgatggtaa atgacaaggc
     3481 cgtgctttca tgtaattatc ttactgctag agtagaaggt cagcatatta caacacttga
     3541 gggtgtcttg gatgaagcaa agaagtttgg agattttctt gcagacgaag gggctgacca
     3601 gtgtggattt tgtgctcctg gatttataat gatggtcttg gcaatgaaaa gagagcttca
     3661 aaatccaaca gaagaagaag ttatagatta tgtaaatggt aacctttgta gatgtacagg
     3721 atataaaggt caattaagag caatacataa atatttaaac acttagactt ttaagtgaaa
     3781 attaagttga tatggggaga gagtatgata actataaagg aatatgtagt tccaaaaagt
     3841 ttagatgaag cttatgaact gctaatatca agaaaaaaca acataatact tggaggatgt
     3901 ggatttttaa aactaggtag taaaaatatt ggttcagcaa tagatttaaa agacttagct
     3961 ttagattata taaatgaaac tgatgatagc atattaattg gagcagatac aagtttaaga
     4021 actttagaat taaataaggt tattaaaaat tactgtaatg gggtaatttc aaacgctgtt
     4081 tctaatatag taggagttca atttagaagt ggagctagag taggagctag cgtatttgct
     4141 aagtatggat tttcagattt aataccttca ttactagtgg tagatgcaaa ggttaagtta
     4201 tataaaaaag gggttatgaa cttagctgat tttttagaaa atgagcttga aaaagatata
     4261 ttaatagaag ttatattacc taaaaaagat gctataggtg tatttgattc tataagaaaa
     4321 tgtacaggtg attttgcagt attaaatgga gcaatgttaa aagaaggaag tatttataaa
     4381 attgcgatag gcgctagacc aagaagagca aaaattgcct ataaagcaag tgaaatatta
     4441 agtttagaaa atgatataga aaaagctggt gaagtatcaa gtgaagaact ttcatttgga
     4501 agtaatataa gaggaagtaa agaatacaga aaagatatga caaaagcctt agtagtgaga
     4561 atgtataata gtattggagg tgagtgtgat ggtaaataaa gaggtaagaa aaatagatag
     4621 tgaagccatt gttactggaa aaccaatata tacagaagat ttaatttttc ataaagatgt
     4681 tttaacaatt aagcttctta gaagtccaca tgcttttgcg aagataaaaa acatagatat
     4741 aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc acttacaaag atgttccaaa
     4801 ttatagatat tcaatggttg gagagtcata tccagaagca tcacctcatg accaattaat
     4861 acttgaagac gtagttagat atgtaggaga tgaagttgct ataatagccg ctgaagatga
     4921 aagggctgct attaaagcta tgaaacttat aaaagttgaa tatgaagtaa tgactcctat
     4981 attggatgca agagaatcgg aaggaaataa aattttaatc catgaagaag atgatttgtt
     5041 ttgtccattt gattttggat tggatgctac aaaaaatatt gtatcgagag aaaaaatggg
     5101 taaaggggat gtagaaaaag agtttaaaga aagtgatgta atagtagaac attcttatat
     5161 gactcaatca caagcccatt gtatgatgga aactcataga gcttatacgt atatagatac
     5221 gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct tatcatatga gaagacaggt
     5281 ttctaaagca ctgggaatac ctctttcaaa agtgagagta ataaaaccaa gaataggtgg
     5341 aggttttggt ggaaaaaaca tagctgtaac agagatatat gcatcatttg taacttggat
     5401 gacaaaaaga ccagcaaaac taatttatac tagagaagaa acttttgcaa tgactaatac
     5461 tagacatcag atgttttttg atataaaaat tggggcagat aaagaaggaa acataaaagc
     5521 tatagatatg aaagcattga acaatacagg cgcttatggc gataatggac cttcagtttg
     5581 ctctgaatca ggtcataatg tacttccaac atataataat gtacctgcaa taaaatttga
     5641 tgggaaaact gtatatacta atttagttcc aggaggagca ttaagaggat atggagcaac
     5701 acaaggtaca tttgcacttg attgtgcaat tgatgaatta gcgcataaac ttaatatgga
     5761 cccaacagaa ataaagttaa agaatattat aaaaaaacaa actgaaggcg gaataatgaa
     5821 ttttccaata agaagttgtg cacttgatga atgtataatt agaggtaaaa aacttattgg
     5881 atgggatgaa aagtaccctg ttagggatat tggaaacaat aaggtgaggg ctgttggaat
     5941 gtctgtggga actcatggtt ctggtatagc caacatagac atggcaatag taaatatgag
     6001 gatggatgaa gatggaactt ataagttgtt ttctgggtct tcagacttag gaactggttc
     6061 tgacacaata cttgcacaaa tagctgcaaa agcattgaat acatctattg atagaatttc
     6121 cgtttataca ggagatacag atatgtgtcc atatgatact ggtgcatacg cttcttgtac
     6181 tacttatgta actggaaatg ctacaataaa atctgctgaa agtttaaaac aaaagatatt
     6241 aaaagtagca cagaagaaac tcgatttacc aatagatgat ttaatactat atgaagacag
     6301 agtttgttat agtgaagatg tagagaagtt tgttttgtta agtgaattag gaaaagaatc
     6361 tgttggtggt ggaaatcaag aagttttata ttcatctgaa tcttatggta tacaagagag
     6421 tgctaaacca ttcttagcag gatttgctga aatagagtta gataaaacaa caggggagtt
     6481 taaagtaata aattatgctt gttctacaga ttgtggtaca gttatgaatc ctgcacttgc
     6541 tagaatacaa gttgaaggtg gtgtaactca aggtataggg cttgcaatgt atgaagaccc
     6601 tagacatggt tctgatggaa agcttcaaac ttctaatcta ttacaataca aagttccaac
     6661 aaaaaaagat ataggaaaca taattgttga ttttgtagat aattatgagc caacaggtcc
     6721 atatggagct aaatctttag gagaaatagt tattcataca cctgcaccag caatagcaaa
     6781 tgcaatatac aatgccacag gagttcgtat aagagaactt cctataactt ctgagaaagt
     6841 ttatatggcc atgaaaaaat taaatcaggg aggtaaataa atgattgcta ttaaaagtaa
     6901 atacttaata tcttctgctg atgaaatata tacaaattct gctgttgtag tagaaaatga
     6961 cattataaaa gatatattgc caaatgaaga agttgaaata aaatataaaa atattgaaga
     7021 aataatagat aaatcagatg ctataataat gccaggattc gtcaatggtc atatgcatca
     7081 atatggagtt ctttctaggg gaataccggc aaatgtacat tttactgact ttgaaggttt
     7141 tttaaatgac tattggtggc catttataga aaatagaatt ggattaaaag aagtgaaggc
     7201 aacaactaaa gcatcagcaa tagaacttat agaatctggt gtggtagctt tttgtgatac
     7261 cctagaagct ccaaatacag aagaaggaac tttaattgag caaggaaaaa tactagaaga
     7321 aataggcatg aaagcgattt tatccttaga aagttgtgaa agaataagct ttgaaaatgg
     7381 acttagatgt ttagatgaaa attctaattt aataaagtgg agtagagaaa atagtaaatt
     7441 aataaatgga ataatgtgta cacatacaag cttcacatgt tctgatagat ttattaagaa
     7501 agctaaagaa gatgcaaaaa aattaaatgc accaatgcaa tttcatttat gtgagagtat
     7561 atatgagcca aactatgctg aaaaacactt tggcaaaaaa gcagttgact actataatga
     7621 tttagatata ttggatgaaa ctgtattagc atctcagtgt gtaaaagtaa atgatgaaga
     7681 aatagatata ttaaaagaaa aaggcgtaaa agtggttcat atgcctctta gtaactgtga
     7741 agtaggtgga ggtttttctc cagtaccaaa aatgattaaa aaaggcataa aagtggcact
     7801 gggtactgat ggatatataa atgatttttt tgaagttatg agaggtgcat ttttaatgca
     7861 taaatcagtt gaagaagatg catcagtaat gccagcaaat ttagtattta gaatggcaac
     7921 agagcatggt gcttatgtat taggacttca aaattctggt aaaatagcag ttggtaataa
     7981 ggctgatata atagttatgg aagatgaatt taaaactcct gtgactcttg ataatatatt
     8041 tgaccagata gtagttcagg gtaaaaaaga atttatatca aatgtataca tagatggtag
     8101 acatatttta aaagaaaaac agttagttga tttagataaa aaagctattg ttaaagaaat
     8161 gaaagaagtt gcttgtgaat tttggaaatt ttaacgaatg taaatatata tgaaaagggg
     8221 aagcagataa tgtataattt tttaggtaaa gaattaaaat caccacttat aataggttca
     8281 ggaccattaa catatagtgc agctggatgc aagatattaa gtgatgcagg agcaggggca
     8341 gttgtgacta agactataag aaaagaaaga gctataaatc cagcaccaca tatggttaga
     8401 aatacagcta atgctctttt aaataatgaa aaatggacag attttgagcc agagcaatgg
     8461 atagattttg aaataccaca aatgaaaaga gatgggactg tctgtatagc ttcaataggt
     8521 catacaattg aagaaagtag cgaattagta gagaaagttg caaatgcagg agctgacttt
     8581 attgaattag tatcttatga ctatagagat ttgataccaa tgcttaaaga tgcaaaggaa
     8641 agagtaaata tacctgtaat agttaaactt cctcctatga tagatgagat aggcgatttt
     8701 gctaaaaaat tagaagaagc aggagcagat gcaataactg cttgtgactc tgtaggacca
     8761 gcatttagaa tagacataga aacagggcaa cctttattag gtggaaatgg aataggatat
     8821 ttaagtggag aaactatcaa gccaataact ttacagagaa tatatgaaat aaaaaaacaa
     8881 gttaatatac caataattgg cttaggtgga tgtgtaagtg gagatgatgc cttagaaatg
     8941 attatggcag gtgctgactt tgttggtata tgttcagtag taattttaaa aggtgcacaa
     9001 gtaatatcta aaatacatga tgatttaaaa tctaatctta atagattagg atataatact
     9061 atagaaaatg cttgtggtat agtacataat catatgggag atgttaatga aagacttagc
     9121 tttgagttta cttatgatga agaaaaatgt actaaatgta agatgtgcga gagagtttgt
     9181 gcatatcaag cgagaaaatt gaatgataag atggaattga atgaagaaga gtgtagatat
     9241 tgtggacttt gtatatcagt ttgtaaacca aaagcacttg gaagaaaaat ttcatgttca
     9301 gagctgaatg tatagattat tttataaaaa acaaagtttt aaataaaaat aagaaaggct
     9361 atctcaaaga gataagcctt ttttgttgtt gaccttattt ttgtttattt gtagttattt
     9421 tagatattaa ttagttttga agtaataaag tgaatcaatt ttgtttaatt aaaattttaa
     9481 taattctgat atatatgtta acattgtata gcatttgtgt aaaatgttat aataattaat
     9541 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
     9601 tgtagttagc ataaaaaatg atatgtcgga agtaaaacaa gaaataaatg tgttaaaaga
     9661 agatatgtcg gaagtaaaac aagaaatgac tgtaatgaaa gaagacatgt cggaagtaaa
     9721 acaggaaata aatgtattaa aagaagatat gtcggaaatg aaacaagaaa tgactgtaat
     9781 gaaagaagat atgtcggaag taaaacagga aataaatgta ttaaaaggag atatgtcaga
     9841 agtaaaacag gaaatgattg taatgaaaga agatatgtcc gaggtaagac aagagataaa
     9901 tataatgaaa gaggatatgt ccgaggtaag acaagagata aatacaatga aagaggatat
     9961 gtcggaagta agacaagaaa tgactgtaat gaaagaagat atgtcggaag taaaacagga
    10021 gataaatgta ttaaaagaag atatgtccga agtaagacaa gagataaata taatgaaaga
    10081 agatatgtcc gaagtaagac aagaaatgac cgtaatgaaa gaagatacat cagaggtaaa
    10141 acaagagata aatgtaatga aaaaagacat gtcagaagtg agacaagaaa taactgtaat
    10201 gaaagaagat gtttccgaag ttaaatcaga agtaaacttt atgcaaaata aaataaataa
    10261 cattaatgaa gatatgaatg gaattaagga cgaggtatcc atagcaaatg aaaaattata
    10321 tggaattgaa attaagattg atagtctaga atgtgaagat aaatcaatga aagagataca
    10381 gatagaacag aataatattt taggaagttt attacataat tctgaaataa ataaagctac
    10441 tcatgataat atagaacata acatagcata tataaaaggt gacactaatt ctataaaaga
    10501 ggatatagct gaaataagaa gagacttaaa tttagtagag ttagccacta gtaaaaattg
    10561 gagtgatatt gtcaaactaa aatcagtaaa gtagtatttt aattcactaa tttatcatcc
    10621 acaaactaag aattttatat aaaaga
//
LOCUS       DAMJYX010000109        10528 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJYX010000109 DAMJYX010000000
VERSION     DAMJYX010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.113"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(33..3461)
                     /locus_tag="PC479_002974"
     CDS             complement(33..3461)
                     /locus_tag="PC479_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805499.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAGDAEGDTNAGDAEGDTNAGNAEGDTNAGDAEGD
                     TNAGNAEGDTNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEINVD
                     FKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTL
                     TPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVP
                     EGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIII
                     NGGAITAIGQESSRNTGYGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLS
                     YSYGGAHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTG
                     GSLKATTFKSLGGAVAYGDLEKKTKVFMNKISLTYLGQDKVSTTLVDDFKMLIDGKIY
                     PYGAPSYTDAQGILYLWFSDQFKGSEVSVNLVVIDKITGEVLKPDDFYIKDIGSGSQF
                     LKQYEEFTIDDTPPYLIIKRYDGLPFEEDVEKSFLDWIFKDGGIPIITPKGEKLTDKK
                     FMTISSQRLNEETMEPDINLGSDDLTKQVDAGKYQLSITSTQFSSIYPFKDAYWGHRA
                     YYKAEIIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQF
                     YINGKEYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDIN
                     YIEGSEDEKKLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDN
                     TGEWKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVD
                     TGSGGYDTGNPNLNKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDP
                     DNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDIDTDGDGKP
                     NINIDTDGDGKPDINIDTDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDTDGNG
                     KPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            3730..4302
                     /locus_tag="PC479_002975"
     CDS             3730..4302
                     /locus_tag="PC479_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2805500.1"
                     /translation="MKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(5315..5977)
                     /locus_tag="PC479_002976"
     CDS             complement(5315..5977)
                     /locus_tag="PC479_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805501.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(5994..8549)
                     /locus_tag="PC479_002977"
     CDS             complement(5994..8549)
                     /locus_tag="PC479_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2805502.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(8789..8977)
                     /locus_tag="PC479_002978"
     CDS             complement(8789..8977)
                     /locus_tag="PC479_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2805503.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(8989..9168)
                     /locus_tag="PC479_002979"
     CDS             complement(8989..9168)
                     /locus_tag="PC479_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805504.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(9173..9430)
                     /locus_tag="PC479_002980"
     CDS             complement(9173..9430)
                     /locus_tag="PC479_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805505.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(9403..9543)
                     /locus_tag="PC479_002981"
     CDS             complement(9403..9543)
                     /locus_tag="PC479_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805506.1"
                     /translation="MVIAISLTLGLITGCTNKVSGTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(9690..10528)
                     /locus_tag="PC479_002982"
                     /pseudo
     CDS             complement(9690..10528)
                     /locus_tag="PC479_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
BASE COUNT         3329 a         1837 c         1122 g         4240 t
ORIGIN      
        1 aaataataaa ataaatatgt acacaataaa tgttatttac gtcttaagta tttatttgtt
       61 agatttttaa ttaaaaaaaa caagctaatg aacattaatg ctaaatctag cattatattg
      121 ctttgatctc ctgtttgtac attttgacct acattactat ctaaactttc atctgtatct
      181 atatttatat ctggttttcc attcccatct gtatctatat ttatatccgg ttttccatct
      241 ccatctatat ctatatttat atctggtttt ccatctccat ctgtatctat atttatatct
      301 ggttttccat ctccatctgt gtctatattt atatctggtt ttccatctcc atctgtgtct
      361 atatttatat tcggttttcc atccccatct gtatctatat ctggtttgct atttcctcca
      421 ttatctgggt ctggtttact atttccaccg ttatctggat ccggtttgct atttcctcca
      481 ttatctggat ctggtttacc attgccaccg ttatctggat ctggtttgct atttcctccg
      541 ttatctggat ccggtttgct atttcctcca ttacctgaat ccggtttgct atttcctcca
      601 ttacctgaat ctggtttgct atttcctcca ttacctgaat ctggtttgct atttcctcca
      661 ttatctggat ctggcttatt tagatttgga ttacctgtat cataacctcc tgagccagta
      721 tctacatttt tatcaggctt ccaataatca tctactccat cattatctgt atctattgct
      781 ggacgattat aatctgtatc agggtttcca tctccattgg tatctatatt tttatcaggt
      841 ttccaaatac catctccatt tccaccttct gtactaggct tccattcacc tgtattatca
      901 gtatctatgt taatatctgg cttccaatca ccactcgtat ctatattgat atctggcctt
      961 ccatcaccat cagtgtctat atttatatcc ggtattccat ccccatctac atctacattt
     1021 gtcatatcta actttaactt cttttcatcc tcactacctt caatgtaatt tatatcatct
     1081 ccaatatatt ttacagtaat agtttgttca gtagtaaaaa tatgatgatt gctatctaaa
     1141 ggtctccatt caatagatgc agtactataa ttatgactct cactaccctt attatgagtt
     1201 tttaattcta ctggatcttt atattccttt ccattaatat agaattgtac gtaacctctt
     1261 ggagaggcac aatctggtcc ttctttctta tctggtgaaa tagtaacatt taatgtaaac
     1321 gtatctgttg gtttaaaaac atcagtttta gctttagact cttctacaac taatgaagtt
     1381 tctgtatcag caggaataat ttctgcctta taatatgctc tatgccccca ataagcatcc
     1441 ttaaaaggat atatactcga aaactgtgtt gatgtaatgc ttaattgata tttcccagca
     1501 tcaacttgtt ttgtaagatc atcacttcct aaatttatat ctggttccat cgtttcctca
     1561 tttaaacgtt gagaactaat agtcataaat tttttatctg tgagtttttc acctttagga
     1621 gtaattattg gaataccacc atctttaaat atccaatcta aaaatgattt ttctacgtct
     1681 tcttcaaatg gtaatccatc atatctttta ataatcaaat atggtggagt atcatcaata
     1741 gtaaattctt catattgctt taaaaattga gaacctgaac ctatatcttt aatataaaaa
     1801 tcatctggtt ttagaacttc tccagtaatt ttatctataa caactaaatt aacagatact
     1861 tcagatccct taaattgatc agagaaccat aagtaaagaa tcccttgagc atctgtataa
     1921 ctaggtgctc cataaggata aatttttcca tcaattagca ttttaaaatc atcaactagt
     1981 gtagtactaa ctttatcttg ccctaaatac gttagagata ttttattcat aaaaactttt
     2041 gttttctttt ctaaatcacc gtatgcaact gctccaccta aacttttgaa tgttgttgct
     2101 tttaatgaac cacctgttac tataacatct cccgttgtag cacttaggtc aaaaatccaa
     2161 tctcccactc ctgtcatata aggtaaaatc gttcctccag ttacaagtat acgatatcct
     2221 ctatttgttc ctggattaac acatccatcc ccaaatgcac taccatgtgc acctccatat
     2281 gaataggaaa gtccaccatt aattgtaata ttcccactat aaccagaacc tgtgctagga
     2341 tctactactg agggacttaa tgcattcttt gttgttgtat agtatcctcc acctattcca
     2401 gctccatatc ctgtattacg actcgattct tgacctatag cagttatagc accaccatta
     2461 ataataatat ttcctccact ttcagtattt ccaccaccaa tacctgcaga ataataattt
     2521 ccaacaactt ttaaacttcc tctattatta ctatctagta gtgtaaggcg atctcctttt
     2581 tttaacttgt ctccattttc tagagttaca tctctaggaa tttttccacc ctcaggaact
     2641 ataaactctc cttcaatatc tctatttaca acagaatcat caataattag agtagtacct
     2701 tttcctgctc tcatacctgg ataatataca tcacctgact ctagcttgtt ttcactacca
     2761 tcttgtaata taacatgtgc atatgcacca ccatctttat ctggtgttaa agtaaatggg
     2821 ctattagatg cagttttaat atttactcca tcaaatatta attcagcttt tacatcttta
     2881 ggtataacaa tcctatgtgt tgttggagta tttggattgt tattttgaat tgtcatttgt
     2941 tttccactaa gaatagttaa aacgccactt gcaaatttaa aatcgacatt tatttctcca
     3001 ccactaattt taaagtctcc tacagacgta ggctcttctg cagtggcttc ttcttctaca
     3061 actactgcat tagcatctcc ttctgcatct cctgcattag tatctccttc tgcatctcct
     3121 gcattagtat ctccttctgc atttcctgca ttagtatctc cttctgcatc tcctgcatta
     3181 gtatctcctt ctgcatttcc tgcattagta tctccttctg catctcctgc attagtatct
     3241 ccttctgcat ctcctgcatt agtatctcct tctgcatctc ctgcattagt atctccttct
     3301 gcatttcctg cattagtacc tccttctgca tttcctgcat tagtactttc ttctatagta
     3361 tctgcgaaag tatttgacgc attaaatgat gctactggtc caagtcccat tgagatactt
     3421 aaaatcattg atagtgcttt ctttttacat ttttgttgca taaactcccc ctccccttaa
     3481 taatttttaa tatacactgt atacatcctt atatacactc taataattct taatatacgc
     3541 tgtatacatt ctaatgcaca ctctaataat ttttaatata tacttaaaac ttatatttgt
     3601 caatgaatat actgtttttt caaccttata tattataagt attcattatt tgctaaataa
     3661 ctacaataaa aattttaatt atataaaatt tatttaattt tttatataat aatactaagg
     3721 ggtaattaat tgaaaaaact agtaaaaata ttaataaata ttatttgcat tattacgcta
     3781 attttctcta gcctttcgat ttacataaaa ttatctgaat ataaaaaagc tgatgaagta
     3841 tatacggaac tcagagaaaa cactatcaat aatagcaaac atcaagaact ttatgataaa
     3901 aataatgatt atagattctg gcttaaaata aacaacgtaa atatagacta tcctgtagta
     3961 caaggatata acaatgattt ctacctaact catgattttt ataaaaatta tcttccttta
     4021 ggtagtattt tcatggatta tagaaataat tttgaaaatg ataagagttt aatagtctat
     4081 ggtcattata tgaaaaataa aactatgttt ggtcagctag aaaactatac tgatgaagca
     4141 tttttcaaag aaaataactt agtagaaata aattataaag tacaaactta tacttatgaa
     4201 attttttcag tatatactgc tgacttaatt aacagagatt atttaagtat acactttaat
     4261 agtaattatg agtttaaaga ttatttgaat tatataacct aaaaatcagt acacaaatct
     4321 gatataaagg tagatatttc agataaaatt ataactttgt atacttgtaa ctatgaattt
     4381 aaagatgctc gtactatagt tcatgctaaa ttaatttcaa aaaaagagac tgataagtag
     4441 gtttgtgtaa atgcagactt acctacagtc ttttttgaac gtaaaattag taagtatgga
     4501 ataatattca tattaaactt ttgtaggcaa agaaatgaaa aatcaaagag aaataattat
     4561 tacagattta gattaccaag ctgaattaag aaagtgtaaa actatggagg atgtagttgg
     4621 tgaagatagt atataatagt ttaacttata aattatcttg gtgatattaa tgaagtttca
     4681 aagtatatat tatttattga tataaataat attaaatctg ttgctaacag atgtaccttt
     4741 gtttggagac aaactattta agaaatataa ctaaaatgtt gaagtagcta cctgacttta
     4801 ttttgaaatg cgaacaagat tttggcatta agataattta gaaagaatca ctggtacaca
     4861 aggatgtgaa cttagaatga atagaagtat tcaggcataa ggagctttca cccaaataaa
     4921 acatagtatg aactttaaaa gaattctaat cagaggcaat caaaatgttc tttcatagtt
     4981 tatacctgta gtgttagaca tagtatttat aagtttcaca aataagataa tcattaataa
     5041 atgtagtatg tatattcatc ctctcaaaaa tattttttaa tcttacactt tgtaaattat
     5101 tctattttaa aatggtattt ttataccttt atttcttatg accgttttca agctcaaatt
     5161 ttgcataaat ataaattaat gaaaagcaag taaaaaaaga actgtcgcac agcaatttaa
     5221 aattactcat tcgacagtcc ttatttcgtg gatttaaata gtggagaaga atcacaagaa
     5281 taatacccac gattaaacct ttatacaata cattttatat gactttacca tcactaatag
     5341 ttattataca atcagccatc ttagctattt catcattatg agttatcata accaacgtct
     5401 gcttgtattc atttacacaa tactttagta aattcatcac ctcattagtt gttttactgt
     5461 ctaaatttcc agtaggttca tctgcaaaaa ttattgatgg cttattggca agtgatcttg
     5521 ctattgcaac tctttgttgt tgaccacctg ataattcatt aggaaattta ttaatctgac
     5581 tttctaatcc taacttagtt attaaatcat taatataatt attatctatc tttcctttag
     5641 caattaaaat aggaagaact atattatcat aaacattgat aacaggtatt agattatagt
     5701 tttggaatat aaacccaaat tctttacttc taagattaga tagcttttca tcacttaaag
     5761 tatataaatc taaattatcc ataaatacat tacctaaagt tggtttatca agccctgcca
     5821 tacagtgaag taatgtgctt tttccagaac cactttgtcc aattattgca ctaaatttat
     5881 ggggttctat ctctaaagta attccatcaa cagcctttat ttcattttct ccttttccat
     5941 atattttttt aatatctata acttttaaca tcttcataca tttacctccc aaattattca
     6001 ttctctttta taccttcaat aatattcata ctttcaaaat ctctattact tacaactact
     6061 gatattaaac atacaaatac agatatagca aagaatagta aagattcctt aattgggaat
     6121 gtaaaagaca taaaattctc tattccatag tttatatacg catcattatt ccaatttgta
     6181 aatttaacta gatttataaa tgatggaatc atagctatta taaagctaaa aactgcatat
     6241 gctaatgctt catatataag catttttttc atatttttaa gactaacacc taatgctcta
     6301 agcgttgaaa tttctttttt tctaattagt agatttgttc ttatagtaca gaaaatatta
     6361 attgatgcta ttattaaaac tagtgcaatt attgctattt gtgatttaat gtaactttta
     6421 tatatacttt ctgacatttc atgataactt aatctactat taaatagaga ccctggtata
     6481 cttcttgaaa ttttctgtat ctcattatta acaatctcat catatggatt ttctaaattt
     6541 atgcctaatt gagtgtattt ttgttctcct gttatgtttt tagagtgaat attacttgtt
     6601 ataatttcta gatctggcat aaaatcgcca tctcccatag caatccattc atcctttaaa
     6661 attacagcca ctcttacctt catctcttta taaacaattg tattattttc ttcaacagga
     6721 atttttacag ttattatgtc tcctatattt aattctttga atataggctt tatagtatga
     6781 tcatcaacta tatcataaaa ataattatat actgctacat ttggatatcc atcagtgtcc
     6841 tttttaatat catctaagct accttcttca acaaagtcat ttaacttttc atctatataa
     6901 tcatcatcat aaagctttaa aactaattta tcttccatta tctctttttt ttctactcca
     6961 ttataggttg taaattcttt gtttatatcc ttattattaa aagtaaacat tccatttgtt
     7021 atttgaattt tatgtatact tttaacaccc ttaatttttt ctatattctc tacatcacta
     7081 tctgtgtatt ttgatatttg cgggtctgca tttactccaa attgcattgt tatattatat
     7141 ctattttgca tatcttcaat tttactgttg acctcttctt gcatacttga gaatgtagta
     7201 atgaatatat atcctcctaa agcaatagac aacatagata tagcagctct aaccttattt
     7261 cttccgatat ttttaagtcc cataaatcca taaaatccaa atattttgct tactagtctg
     7321 tctattccaa gtctacttct gtgattttta attttgtctg tatttattat tgattcaata
     7381 ggtgatatct tgccgatttt aaaagttggt ataatacaag atataaaaac agaaatcatt
     7441 ccaaccaata tagcttttat aactatttca ttatttatat ataatttagg gttaagacca
     7501 gcttcactgt ataaaacttc gttataatta ctaattccta tatatgaata tgtaacccca
     7561 gctactaacc caagtagtat acctattatt gttataatta atgcttgata gattatcata
     7621 caccttatgt ttcttttctt tgcgcccatc agtctaagca gtcccatttc ttttactgat
     7681 tgatttagtg taatattgaa aatattaaat atgaaaatta tagatactgt agttattaaa
     7741 acatttcttt taaaaacatt cggatcattc ttatcatctt ctacataacg aagttccctt
     7801 acaagaggta catttggtag aaaacttttt tgtcccaact tatatttttt cataagataa
     7861 tcacattcac cttcaatatt tctccatcca cttttaaaac ttaatatact attataacta
     7921 atcgcatcat gaggtataat gttatttgtt tcatcattgc cataagttac tgcttcgtat
     7981 tgccaaacca catcatagta cccttttggt ttttccataa ctcctactat tttaaattct
     8041 ttatttatag aaaataattg attacttccg ttagtatctt tatagttctt ttttattgtt
     8101 aaatttaatg tagatcctac tccttcactt aaattcattg cttctagtgc ttttttctct
     8161 aatactattt cattgttgtt tttaggtagt ctaccttcta ttattttgtt gtatctactt
     8221 tttagatact cttcgtttga tgaagttaat ataaatgagt ttcctttttt atctgaaata
     8281 cgtcctagat tttgtaccga attacccttt gatacttttt catcagtttc taatttttta
     8341 gcatcatcaa gtgtcaaatc actaaactct acatggtatc cactgtaaag ttcatgtatt
     8401 gctactacat tgttttcatt ttgtgaatct tttataacac taggtccaaa tatcaacata
     8461 acacctaaca taactcctat tattaaaagt aatgttctaa gtttattttt ctttaaatat
     8521 gctagtgaca tttttaagta aatccccata taatcctcct attttactat aaattattta
     8581 tccaactcat ctgttaagta gattggtgga tattttaaat cttttaattc ttcattattt
     8641 tttgaagctt actaccatct tcctcatgat aaacttgcag tatcacctta tttacatgta
     8701 gtgcataata atcaccatta taaaaatatt agtatttata acctggataa tttttgtaaa
     8761 atgatttata aagtatccca tcctaattct attttgtata cttttctttt gtaatacgct
     8821 ctatatcatc acctgaaact ccttgatatg tcatatattt atctatatat ttttcagctt
     8881 cattccattt tctaaaatag ttgttgtata taataaatcc tattatggct ataatgctta
     8941 taattaaagt taataaaata gctttgtatt ttctcatagt gaatcttcct acttcaattt
     9001 aaattcttta ataagtttta attctaaaga cttagtttta tcatcaaatt tcttaacttg
     9061 aatttttata tctcccaaat tatcaacctc tacatttact tcacctttat caaatacttc
     9121 ttcaattttt ctaaaaaatt ctttctcact tatttctata tgaatcaatg atctatatgt
     9181 tataaatggc cattatgaat ttatcatctt ctgacagctt ttcattataa gaaaaatctt
     9241 taacacattg aattgataat atttccttcg tatccttctt ttccttagtt attgaataat
     9301 gtaaaacttg gctaggttgt tcttttgtat actccaagtt tcccaaagac aaaacaaaac
     9361 ttccatctga tttattttca gctaccctaa gaccactatt ttctattgat tctgatattt
     9421 tctttgacaa gtcttcttga tactagaaat ctgttcaata gtatatattt tttcatttgt
     9481 acctgaaacc ttatttgtgc atcctgttat taaacctagt gttaagctta tagctataac
     9541 catagttatt gtaatttttt tatcttcata taatcctcct tatatattga aaactcgtta
     9601 tttctcataa aaaaaagacg ctatcttttt ctattaaaag ataacatccc tatatgttta
     9661 gtatcaatat atgttcccca actggtaggt taatcgaccc aactatcaaa attttgattt
     9721 aatggtatat aaatattcaa tataaatttg tcttttttat cttcaaatga taaatctcct
     9781 ctatattttt ttatagaaga tttgatgctt taaatcccta ttccatgtac aagtttatct
     9841 tttttatctg ttaataataa acttttattc tttctaattg aatttatttt actattctca
     9901 catttaatta cgaaatagga ctttgagata gttcctttta tttgtatata tttatcaata
     9961 cttttatcat gtatcttatc gcaagcttct attgcattat ctaagtattt gcaaatatat
    10021 tagatacatc tattggtttt ataaaattca ctttggaaaa attgatatca caaatgaatt
    10081 taatttttct tttttcactt acatcacttt tttcatttat tattatatcc aatataacat
    10141 tacctgtatt gtaaattgat ttaaattctt taatttaatc ttttaaacta tttacatacc
    10201 catcaatttt ccttttttat atttgagatt ttctatacaa tatatatgat tatttatatc
    10261 atgataaagc ttcttaactt tagcgtgtga ttcttgaata cttagataat gagaatactg
    10321 catatctaac ttatttttta ttacttcatt ttgcaatttt aacttatttt ccttaattat
    10381 ttttataaac actataataa tgactaagct actagctagt attaacctag gtatcacaat
    10441 atacccaact atatatattt cctaataccc accaaatcga tacatttttc tatttatata
    10501 aattattaat atagtaaata aatttgtt
//
LOCUS       DAMJYX010000110        10475 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJYX010000110 DAMJYX010000000
VERSION     DAMJYX010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.114"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            178..3075
                     /locus_tag="PC479_002983"
     CDS             178..3075
                     /locus_tag="PC479_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2805507.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3396..3608
                     /locus_tag="PC479_002984"
     CDS             3396..3608
                     /locus_tag="PC479_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2805508.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3621..3842
                     /locus_tag="PC479_002985"
     CDS             3621..3842
                     /locus_tag="PC479_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2805509.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3942..6098
                     /gene="feoB"
                     /locus_tag="PC479_002986"
     CDS             3942..6098
                     /gene="feoB"
                     /locus_tag="PC479_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2805510.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6124..6276
                     /locus_tag="PC479_002987"
     CDS             6124..6276
                     /locus_tag="PC479_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2805511.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6456..7166
                     /locus_tag="PC479_002988"
     CDS             6456..7166
                     /locus_tag="PC479_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2805512.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7251..8021
                     /locus_tag="PC479_002989"
     CDS             7251..8021
                     /locus_tag="PC479_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805513.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8014..8745
                     /locus_tag="PC479_002990"
     CDS             8014..8745
                     /locus_tag="PC479_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805514.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8779..9738
                     /locus_tag="PC479_002991"
     CDS             8779..9738
                     /locus_tag="PC479_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2805515.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9836..10432)
                     /locus_tag="PC479_002992"
     CDS             complement(9836..10432)
                     /locus_tag="PC479_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2805516.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4033 a         1095 c         1896 g         3451 t
ORIGIN      
        1 tataagaaac taatattaga tagtgtattt aaatagataa taataaattc attatagttg
       61 aataagaata ttatatgaga atattgcaat ttgatatatt taccattaca ataaaagtag
      121 ttacaactag aaaacgaaat aagttaaagt aagttggcaa attggaggtt tttaaatatg
      181 aaagcattta aaaagcactt cttgatactt tctttgatat ttttacttat tatatcatgt
      241 tttaacaata tactatgctt tgctgatgag tctgaaaatt caaaaaaaat aagagttgga
      301 tattgtgatg actacggaat aatatcctca tcaaaagagc attcttatac aggatatggt
      361 tatgattatc taagagaaat ctcaaaatat acaagatggg aatatgaatt tgtaaaaggg
      421 tcttgggaag aatgtttaga cagactagaa aagggtgaaa tagacttact cggaccttta
      481 cagaagaatg atgaaagaaa taaactattt aattttccta aactaagttc aggatatgaa
      541 tatagtgcac tatatacaaa ggatagtaac aataatatgt tttatgaaga tataagttcc
      601 tttaagggaa tgagagtagc tgtacttaga ggaaactttc ataatactgc atttgagaag
      661 tatagagaag aaaataattt ttcagttgaa tatatatact gtaattctat tgatgaatta
      721 attgagtccg tcaatgaaaa aaaggcagat gcatttgttt gtggaagtat tataaatgct
      781 aaaggaatga aaatagttag caaattttca gtagaacctt tttattttgc tactgcaaaa
      841 gataaaccta atttagtaaa agaattagat tatgctttaa aagaattaaa aataaatgat
      901 atgtattatg agttggaatt atataaaaaa tatttcaaaa gagaggtcaa taattccata
      961 gcatttactc ggcaagaaat gaattttatt aaagcaaacc caaaacttac tatggtatat
     1021 gattcagagt ggtctcctgt agagtattat gatgaagaaa gcaattcatt taaaggtata
     1081 agttccgatt taatgtcaat tatatcaaaa aaatgtggta taaaatttga atatattaaa
     1141 acaaaaaatt ataatgagag tcttgattac ataaaaagtg gtaaggcaac tatgctatgt
     1201 ggctcaatca atgaaaatga taaggtaaaa cgatttaata tgaaattgac aaatccatac
     1261 ataaatattc caatgattat ggtaggaaaa ttggatacca atctcaataa tgatttaaat
     1321 attgctttaa ctagtagtta taagtctatt gattcttata tagaaaagag ttttgaaaat
     1381 gctaagacaa catcttataa gagtgtagaa agttgtctaa acgctataaa tgaaggtaaa
     1441 gcaaacttaa tgatacttag ttcttatcag tttgatgaac ttttaagaga aggaaagagt
     1501 gagaatttat ctgttatatc tgttttagat acaagctatg ggatgcgtat aggggtatca
     1561 aataaaactg accctatatt agtgtctatt ttaaataaat caatagacaa aattactgaa
     1621 gaagaattaa gtgattgtat atattctaat actattgaca aaccttataa agtaccattt
     1681 ggtgtgattt ttaaagaata ttcaatacaa attatttctt ttgtatgtat tttattctta
     1741 attgctatta aatatataat ttataataaa aagaagaaag aggattatct aaggaagata
     1801 gcatatacag acccattgac aggagcagat agtatagata aatttaaaat aaattctaat
     1861 aaactgtttg ctaagaataa tccagaagaa tatgcgttgt tttatataga tgttgacaaa
     1921 tttaaatata taaatgatat gtttggatat gatatgggaa atgatacttt gatacatata
     1981 tcaaatacta ttgcaagtga attaaaggaa gatgaaattt ttgcaagagt ttctgctgac
     2041 cattttgttc ttcttattaa gtataagaca gatgatgata ttaaaacaag gttaaacaat
     2101 atttacaata aggttcaaat acttagtaat cctaaaataa actattataa attgatttta
     2161 gattgtggta tatataaaat aagtaaaagt gataatgaca taaatactat tatggataga
     2221 gctaatactg caagaaaaac tataaaaggt ggtcataaaa attcatttgc tttttacgat
     2281 aaggaaatgc acaaaaaaat attaaaagag aaagaaattg aaaattcaat ggtagatgct
     2341 cttaataatg gagaatttat tgtttatttt caacctaaat atagcctttc agattatcaa
     2401 attatagggg cagaggctct tgttcgttgg gataatccac aaaaaggtct aataccacca
     2461 atagaattta ttcctgtatt tgagaggaat ggttttattg taaatattga tttctatgta
     2521 tttgaggaag tgtgtaaaaa gattagagaa tggatggatg aaggacaaaa agtagtacct
     2581 atatctgtaa acttgtctcg aatgcatttt gtaaatagta attttataga ggagtttaaa
     2641 ttaattgtag ataaatacaa aattccgaca agacttatag aattagagct aacagaaaca
     2701 gcagtattag ataatataga aggattactt gatacaatga acaatttgaa agaaaaggga
     2761 tttgtaatct ctatggacga ttttggtaca ggatattcct ctcttaatct tttaaaagaa
     2821 ttgcctgttg acatattaaa actagataga gcatttttta cagaaaaaga tgagagtaat
     2881 aatgaaaaaa ttgttatatc taatgtcatt aaaatggcaa aggaactgaa aatgaaagta
     2941 atatcagaag gtgtagagac tatttcgcaa gtagaatttc taaagcaaat tgggtgtgat
     3001 atggtacaag gatatttgtt ttctaagcca atgcctgtaa aagagtttga aaaaatcgct
     3061 tttaaaaaag aataaagcaa tgtgtaaata atgacatata taataaaggt gctttaatat
     3121 tagttgagta tactatttta gagcatcttt atttttgtat taatttttac gattttatta
     3181 ataaaataaa tcaaatgaga aaaaaaatca aaagaataca ataatcactt gcaaaaaata
     3241 tactattgtg atagacttaa atcaaaatta ggaattgtaa aaagaaggaa aaaataggat
     3301 gttattttaa tagattaaga tttaaggtat tgtaatttat atataagata acgattgctt
     3361 gtagatttaa attactttaa gaggagggat attatatgat gccattgata ttatcaaata
     3421 caggtgaaga agtagttata aagaaaattt cgggaagtga taagacgagg acttttttaa
     3481 atagtatagg ttttgtagtg ggtacaaatg ttaaaatagt ttctaagata gatggtaact
     3541 taataataga cgtaaaagat actagagttg cattagataa aaaaatggca agtagaataa
     3601 taatataggg aggtaatgac atgaacagac tgaaggatat taagtgtgga gagactgtaa
     3661 aagtcaaaaa gttagaggga gaaggagcta cacgaagacg tataatggat atggggatta
     3721 caagaggtgt taatatattt ataagaaaag tagctcctct tggtgaccca attgaggtaa
     3781 cagtcagaga ttatgaacta tcaattcgta agtcagatgc agaaaaaatt attgtggaat
     3841 aataaagatt aattagagta gggttaatgt aattatataa tgagaatgat tttctgtatc
     3901 acaaacagtg atgtatttaa ataataatgg agggaatgga aatgtcaata aaaataggtc
     3961 tcataggaaa tcctaactgt ggtaagacaa caatgttcaa tgggcttaca ggctcatcac
     4021 aatatgttgg taactggcca ggagtaacag tcgaaaaaaa aggtggaaaa ttaaaaggaa
     4081 ataaagatgt ggaaatagta gatttaccag gtatttattc tttatctcca tatacattag
     4141 aagaagtagt tacacgtaat tttatgctag atgataagcc ggatgcagta ataaatattg
     4201 tagatgcatc aaatatagag agaaatttat atcttacaac tcaagtttta gagctaggta
     4261 taccgaccgt tatagctctt aatatgatgg atattgtaaa taagaatggt gataagataa
     4321 atattaaaga actatctgaa gttataggtt gcccagtggt agaagtaaca gcagttaaag
     4381 gtcaaggaat tatggaggct gctgaaaagg cagttgaact tgcaagtagt aacaataaat
     4441 taaatttcaa attacccttt gttgatgaaa gtaaggatgc aatagaaaaa attgaaaaaa
     4501 taatagaaga aaagactcca tatatagatg tagaaacacg ctggttagct ataaagctat
     4561 ttgagagaga tgaaaatgtt atacaaaaac ttgatatttc taaaacaata ttgaatagta
     4621 ttgaagaaat aactagaaat tgtgaagatg aattggatga tgatagtgaa agtataataa
     4681 cagccaatcg ttatgaattt attagttcaa taatttcaag tataataaag aagaatcgaa
     4741 aaggtaagga aactgtatca gataagatag ataagattgt aacaaatcgt atactagcat
     4801 tacctatttt tgcacttata atgtggggag tttactatat agcagtaagt tcattgggta
     4861 ctatagcaac tgattggaca aatgatgtac tgtttggaga aattattcaa ggcaatgtaa
     4921 gtaattttct agcttcttta aatgtagcag agtggttaca aggacttgta gttgatggtc
     4981 taattggtgg agttggagca gtactaggat ttgtaccaca aatcatgctt ttattcttat
     5041 tattatcaat attagaagat tgtgggtaca tgtcacgtgt agcttttata atggatagaa
     5101 ttttccgtaa gtttggtctt tcaggaaaat catttatacc aatgcttata agttcaggtt
     5161 gtggagttcc aggagttatg tcaactcgta ctatagagaa tgatagagat agaaaaatga
     5221 ctattatgtt aactacattc attccatgtg gagctaagat tccaataata gcattatttg
     5281 caggggcatt atttggaggt gcttcatggg tagctccatc aatgtatttc ttaggtattg
     5341 caatgataat catttgtgga attatattaa agaaaactag tttatttgca ggagaaccat
     5401 ctccatttgt tatggaatta cctcaatatc atattccaag tgcaaaaggt gttttaatac
     5461 atatgtggga tagaggaaaa gcatttataa tcaaagcagg aacaattata tttgttgctt
     5521 gtggagtaat ctggttctta caatcattta attggtcatt acaaatggta gatgctggag
     5581 atagtatatt agctagctta ggaaatatag ttgcaccaat atttgctcct cttggatttg
     5641 gaaattggca atcatcagta gcaacagtta caggtctagt agctaaggaa aatgtagttg
     5701 gaacatttgg agttttattt ggtatatcag atgcaacaga gcaagaccca acacttcttg
     5761 cttcagttgc aagtatgttt acagtagcta gtgcgtttgc atttatggca tttaatatgc
     5821 tttgtgcacc ttgttttgca gctattggag ccataaaaag agaaatgggt tcatggaaat
     5881 ggacatggat tacattagga ttccaaactt taacagcgta tataattgct cttcttataa
     5941 atcaagttgg aagtttagtt ttaggtactg gaggaagtat tgctggggca ataatatcta
     6001 tatttatagc agtagcagta gtctttgtag ttttaactta ttcaaataaa aatatgaaaa
     6061 aagaaaaaat gggtaaatta agttatatga aaaattaatt catataaata aggagtgatg
     6121 aatatggcta catttattat agcagctatt gtagtagtat taatggcact agctgtaatg
     6181 tatatggtta aaaattcaaa aaagaatggt agcagttgtg gatgtggttg ttctggatgc
     6241 tcaagtagta agagttgtca tagcgctaag agataaatag gataagagcc tgataaaaag
     6301 ttaaattgct ttattatcag gttcttattt tttgtataaa taacaaattt catatttgaa
     6361 gtaggttaga ctatgctgta attttactgt ataattatat ataaatatat gattaggagg
     6421 gattattgct aaattaagca atataagtga atattatgtt taaatttgat gttacaaacg
     6481 agcttatgtg tatagcaagg ggaagtggta aattttttgc aaaaaaaggt gctatggtag
     6541 cctttaaagg aaattttaat tttgaaaaat tgctgttagg tccaagtaat ggcggtggat
     6601 tgggaagagc attattaggt catgttcaga ggtcattgac aggagaacaa atgccaataa
     6661 tggtggttga aggtgaaggt gaaatttacc tagctcaaaa tgcttaccat gtagatgtaa
     6721 tatcaattga cccaggtgat tccatttatg ttgagagtga aaacttattg gcattttctg
     6781 aacaattaaa ttacagtgta aaattgattg gttcaggggt aatatctcaa aaaggtcttt
     6841 ttactacaca tttagtaaat aaaacaggtc aaaatcaaga tgttgctata ataacagatg
     6901 gtaatcctct aattcttgaa ggaccttgtt gtgttgaccc agatgcatta gtagcttgga
     6961 ctggaagaga accatatcct aaagtatcaa aactatcatg gaagactttt attggtcaga
     7021 cttcaggaga atcttatcat atggaatttg ttgaacctgg acaaatagtc attatacaac
     7081 catctgaaag attgtctggc ttaaaaacag attctacacc ttctgataga atgagttcag
     7141 gtggattaag acttggcata gattaaatct tatgatatag cttaattatt atttaagata
     7201 ttaaaaaata aaaagggtag cagggataga tatgttaaaa ggagttttat atgtttgaga
     7261 aaattcataa aaggcttaaa ggatattata tttttttaat aattttaatt ttatggaaga
     7321 tagtatgtgt tttgggtata tggagtagct atattctgcc tcctcctgaa atagtcttag
     7381 atacttttat aaaaatgata tatgacggtt ctattttctt aaatgtttat gccagtgtaa
     7441 gaaggatatt aataggtttt ttaataagtt cattgatggc tataccactt ggagttttat
     7501 ttggagcaaa taaaaaaatg tatgaatatt tcaaaccact atttgaattt atgcgaagta
     7561 cacctccact agctttggtg ccaatgctta ttttatggtt tggtataggt gaagaatcta
     7621 aaattataat tattattttg gcatcctttt ttccaatatt tttaaataca ttgaaaggta
     7681 taaaaaattg tgacaataaa ttaatagaag taggtaaatc ttttaattta tctaaaaaga
     7741 gaatttttta taaaataata atccctaatt ctatgctgga tattgttgtt ggacttagtt
     7801 tagggcttgg gtatagtttt agggctataa ttggagcaga gcttattgca gcttcttcag
     7861 gactaggata tttgatttct gatggaaaag atatgtctag aactgatgtt gtattggtag
     7921 gaatttttgt tattggattt ttaggtatac tttcagatta tctattttca aagataataa
     7981 caaaatttag caaagggaag caggttgatt taaatggtta gaagtggatt tttgctagag
     8041 aatatatata agaaatattt agttgataat aaagagcatt tagttcttga taatatatct
     8101 ttaaatatat ctagtgaaga gataactgtt atattaggtg aaagtggatg tgggaaaaca
     8161 acgctactta gaattttagc agggcttgaa aatgctacta gtggaaacgt atattttttt
     8221 aataatgata aaaagtgtac tccaaaagta ggaatggtat ttcaagaaag tagacttatg
     8281 ccatggctga atgtaagtga aaatatactt ttacatactg aaaaagataa tagaaataaa
     8341 gttgatttag acaagtattt aaaaatgatg aagcttgaaa agtttaaaaa ctcatatcca
     8401 aatgaattat caggaggaat ggctcacaga gtaagtatag caagagcact ttccttcaat
     8461 cccgatattt tgctgatgga cgaaccattt gcagcactag attacttcac aagaagaaaa
     8521 atgcaaaaag aagttgtaaa tatccataaa aatactaaaa aaggtgtagt atttgtaact
     8581 cacaatattg aagaagctat ggaaatagca aagaaaataa ttgtttttag taaaaataaa
     8641 aggattaaac aatttagtgt cgaagatgaa tataatagag atttaactaa aaactattat
     8701 ataaacttga aaaaagaaat attaagggaa ttgggggagt tttaattgat taaaagaaac
     8761 aaaaagattt tagcagtaat gacatcttta ataatggtgt tggttgggac tgtaggatgt
     8821 tcaacaacaa atgataagaa agatgaacct gcaaaaaaag aggctagttt acctaaggaa
     8881 ataaatttaa cttatgtaaa atcaccactt aatgttccta gtatattaga aaaaaaggat
     8941 gatttatttg gaaaggaatt taaaaaggac aatataaaag tcaatttcca tgaattgaca
     9001 acaggaccag agcaaactca agcattagca gcaggtgagc tagacttttt acatgcatta
     9061 ggaggaacgt ctgcaataat agctgcatca aatggtgttg gtcttaaaat tacgaatatt
     9121 tatagccgtt caccaaaagg atatatgctt ataactaata atgataatat taaatctata
     9181 aaagacttaa aaggtaaaaa aattgtaggt ccaaaaggga ctatacttca tcaactgttg
     9241 attgctgcac ttgctaaaga aggctatact atagatgatg ttgaatatgt aaatatggat
     9301 attcctagtg ctgcttctgc attagaaagt aaaagtgctg atgtagcaat gcttgcaggt
     9361 ccagtggcat tgaaaacgat aaattcaggt gccaaaatga ttgtaaatgg agaaggatta
     9421 gtaagtggaa taatagttac tgctgtaagt gatgactttg ctgaaaaata tcctgaatta
     9481 gtaaaaagat ttatgaaggt tcatgaagaa actttaaaat atatgaatga aaacaaggat
     9541 gaagtgatgg atgttgtatc taaagaagtt ggtttatctt tagatgaaac aaaagaaatg
     9601 tattcttggt atgactttag ttctaaaata acggacaagg atataaaaga attagaagat
     9661 acacaggaat ttttgatgtc taatggaatg caacaaaaga aaataaatat taaagatatg
     9721 ttgtacaatc aaaattagaa tactttaaaa tattaaagtg gaaaatacac ccttatttat
     9781 tatggaaact gtatggtaaa agagggtgta tttttattat tatacaaaaa cacaattaaa
     9841 aacagcttac tattttatct acacttatgt agtctagtaa ttttctcatt ttcttacggt
     9901 gaagtaaaaa cttcgaatat gtgtcctcgg attgttttgt gtcaccataa actttccaat
     9961 atttaatcat agaaacttca taattactat atcccataaa tccaatcaca tctttaagag
    10021 gatatcctaa aaaaatacct atttcatcag gaaaaacatc actcttaagt ttatctacta
    10081 aatgctctaa ataagcatta acattcgtat tttgtttgta tcctaaaaac tttaaaaagt
    10141 ttactgtttt tttacatttt aatttttctg ataatgcttt tttatttaca aataagattt
    10201 ttaatccctt tttttcttta tcaataacta taaaatctat tttcttacac ttatatagat
    10261 atttataaat atcattaatt ttttcctctc tatttacatc agtaaacgaa atatttatta
    10321 tttcagcagg tttagagcct aaaaagactg gacctaaaac ttctaaaagc cattttatat
    10381 atgatgattc aattttattt ttacaacaaa attgtgaatt acaacaagac ataaaagata
    10441 acctccttca aatgaaaatt aatatcaagt aatta
//
LOCUS       DAMJYX010000111        10310 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJYX010000111 DAMJYX010000000
VERSION     DAMJYX010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.115"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            374..1534
                     /locus_tag="PC479_002993"
     CDS             374..1534
                     /locus_tag="PC479_002993"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2805517.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1536..2666
                     /gene="glgD"
                     /locus_tag="PC479_002994"
     CDS             1536..2666
                     /gene="glgD"
                     /locus_tag="PC479_002994"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2805518.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2695..4137
                     /gene="glgA"
                     /locus_tag="PC479_002995"
     CDS             2695..4137
                     /gene="glgA"
                     /locus_tag="PC479_002995"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2805519.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4151..6571
                     /locus_tag="PC479_002996"
     CDS             4151..6571
                     /locus_tag="PC479_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2805520.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6576..8441
                     /locus_tag="PC479_002997"
     CDS             6576..8441
                     /locus_tag="PC479_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2805521.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8686..9339
                     /locus_tag="PC479_002998"
     CDS             8686..9339
                     /locus_tag="PC479_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805522.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9435..10034
                     /locus_tag="PC479_002999"
     CDS             9435..10034
                     /locus_tag="PC479_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HDF2805523.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
BASE COUNT         4148 a         1004 c         1873 g         3285 t
ORIGIN      
        1 ccttggataa ttatttaaca aatattagat tatattttgc tattacgaat ttaacaaatt
       61 aaaaaataag catgttcata tgacacaaag gatttataaa aaatgttttt atatttataa
      121 aataaaattt gtattatttt ttaaccgata attgaatatt aataaatact atagtgctta
      181 tggaaaataa tgtatacgtc ggtaagtcca ttatggggta tacattatat caattgtata
      241 gttgatatta ttttattttt attaaggtat tatttaagca acatttatac acgacattta
      301 ttgactgata aagtttataa aagtaaaaat gcaattataa atattaatta ctagatgagg
      361 ggagtgtcat taaatgaaaa aagagatgtt agctatgatt ttggcaggag ggcaaggttc
      421 aagattaggt gtttttacaa aaagaattgc caaaccagct gtatcatttg gaggtaagta
      481 tagaattata gattttgtgt taagcaattg ttcaaactct ggtattgata ctgtaggtgt
      541 tttaactcaa tatagaccgt tgatattaaa ttcacatata ggcatgggaa gtcattggga
      601 tttggacaga ataaatggtg gtgtctatgt tttacaacca tttatgaatg aaaaagaagg
      661 aaattggtat aatggtacag cacatgcgat atatcaaaat atggactttg tagataccta
      721 taatccagaa tatgtattga tattatctgg tgaccatata tacaagatgg attatagcaa
      781 aatgttaaaa tttcataagg aaaaaggctc taaagctaca atagcagtga ttgaagttcc
      841 ttgggatgaa gcttcaagat ttgggataat gaatacaaat gaagattcaa gtatctatga
      901 atttgaagaa aaaccaagtg aacctaagag caatttggca tctatgggag tatacatatt
      961 tgattggaaa atgttgagaa attattttaa ggaagcagaa aaaaatccag aaataaatta
     1021 tgatgatttt ggaaaaaatt taatacctaa aatgttagaa gataatgttg gaatgtatgc
     1081 ttatccattt aaaggatact ggagagatgt aggaactata caaagtctat gggatgctaa
     1141 tatggatata ataaaatctc cagaaacact tgatttagca gaccctaagt ggaagatata
     1201 tacaaatact atggcaatgc ctccacaata tataggtaaa aatgcaaatg ttcatagatc
     1261 aatgatagct gatggttgta gaattttagg tgaagtaggt aactctgtat tatcacatgg
     1321 agttgttgtt ggtaaaggaa gtaaagtaat tgattcagta ataatgccaa atgttgtaat
     1381 aggtgaaaat gtaactatag aaaaagctat gataggtgag tgtgcaacta taaatgataa
     1441 tgttcagata aagaatgtaa ataatgaaat aaatgtagtt tctgagtatg aaaatatcga
     1501 acctagatgc gtattaatag aaggaggtct ttaagatgag aaatgaatgt ttagggataa
     1561 taaatttaaa caaaaaagga gacccagcaa taaacaaact taactatgga agaccaatag
     1621 catctacacc aatagctgga agatatagaa taatagactt tgctttatct aatatgataa
     1681 attcaggaat cactaaagtt ggaatattcg caaaagaaaa atacagatca ttaacagacc
     1741 atataggtag tggtaaagat tgggatttaa gtagaaaaaa aggtggccta tcaattttta
     1801 gtccagaaaa cactaagtat agaaatactt actcacatag agaaggtgat atatatacta
     1861 tcttagctaa tctagattat atagaaaaga gtgaagaaga atatatttta atagctccaa
     1921 gctatatgct atgcaattta aattattctc aagcattgga ataccataaa aaatctcata
     1981 atgatataac tataatatat aaaaatgtaa ataatgcaaa taaagatttt gctggtaact
     2041 taactctaaa tttagatagt aacaataggg taataaatgt aggaaataat ttaggtaagt
     2101 tccctagagc aaacatatgt atggagacat atattatgaa gcgtgaagat tttgttgaat
     2161 gtatatataa cattgtaaat aaaggcaatt attgttattt agaagaattt attattgaag
     2221 aagcagaaaa catgaaaata ggagcctatg aatatacagg atatctaaaa tgtgtaaatt
     2281 ctgttgaatc atattttgaa atgagcaaag atttattaaa tttagaagtt gcagatgaat
     2341 tattatattc tgaaagaaag atatttacaa aagaaaagaa tgaatcacct acaatttata
     2401 cagatagtgc taaagtagaa aatagcttta tagcatctgg ttgtttaata gaaggtactg
     2461 taaaagatag tatcatattt agaaaggtaa atgttgaaaa aggaacagtt atagagaact
     2521 caatagtaat gcaaaattgt gtaataaagt caaatgcaaa actatataat gcaatattag
     2581 ataaaaatac aagtgtatca aatgggaaag aattaagagg agatgaaaaa tatcctcttg
     2641 tagtggaaaa gaatacaaat atataaataa attttttaga agggggaatg actaatgaag
     2701 gttttttatg taacagcaga gtgttggccc tttgcaaaaa ctggaggatt aggagatgtt
     2761 tcttatgcat tgcctaaaga attaaaaaaa gaaggtgtag atgtaagggt aataatgccc
     2821 aaatattcaa ctataccaag ttatttaaaa gatcaactaa aagaaatagc tgtgtttagt
     2881 gtacgtgttg gatggagaaa tcaatactgt ggtcttttag aaatggaatt ggatggtgta
     2941 aaattttact ttatagataa tgaattttat tttagaagag aagatgaaag aaagagtatc
     3001 tatggatatg gagatgatgc agaaagatat actttcttta cagatgcagt tttagaagct
     3061 ataagtagaa ttgattttta tccagatgta atacatataa atgattggca tacagggatg
     3121 ttaccattaa ttctaaaaga aagatatgca acccttgagg ggtataaaaa tataaaaact
     3181 atgtatacta tacataatct ccaatatcaa ggtgtatttg ataagcatgt tttatatgat
     3241 atattggact taccacaaaa atactttgat aatggagata tagagtatta tggctcaata
     3301 aattttatga aagctggtat aaattttgca gataaaatta taactgttag tcctacttat
     3361 gcaaatgaaa tacaaacatc tttttatggc gaacaattag atggattact tagaaaagag
     3421 tcaggaaaat taaaaggtat attaaatggt atagattatg atttaaatga ccctgcaaaa
     3481 gataaagata tatttgttca ttatgatgta gactcaattg acaaaaaagt agaaaataaa
     3541 ttgagattac aagatatatt gggactaaaa aaagattcat ctattccgtt gataggtata
     3601 gtttcgagat tagtttctca aaaaggattt gatttaatag catacatgat gccagaactt
     3661 atgagagaag atttacagat tgtcgtttta ggaactggag aacatcaata tcaatcaatg
     3721 ttcaactatt acgattctaa tttttcagat aaagtatcag ctagaattac ttttaatgca
     3781 tcattggctc aacagatata tgctgcaagt gatatgttct taatgccatc attatttgag
     3841 ccttgtggta taggtcaaat gttagctatg agatatggtt ctttaccaat agttagagag
     3901 actggagggc ttagagatac agtaacacct tataataaat ttactggaga aggcaatgga
     3961 tttagtttta aaaattacaa tgctcatgag atgtttttct gtttgaaaaa tgcaatcaaa
     4021 gtatttaagg acaaagaaaa atggattaaa ttagttgaaa atgcaatgaa aacagataat
     4081 agttggaaaa aatcagctaa ggagtatata gaaacatata gagacatatg tgattaaagg
     4141 agtggttttt ttggtaacaa taagcaaaaa gaaattcaag caaaaatttg aagtaaaaat
     4201 gtatagctta tatgcacaat ctatcaagga agctactgat gaacaattgt taaatgtatt
     4261 atgtagtcta ttgaaagatg agatagcaaa aaaatgggtt gctacaaagt tagataagaa
     4321 aaaagaagtt tattatttta gtttagaatt tttaataggg agacagttaa aatctaattt
     4381 attaaattta aatattgaag aagaagttag agaaggttta tctgaattag gaatcaactt
     4441 ggatgattta attgaagctg aagtagaccc agccttagga aatggtggac ttggtagatt
     4501 agcagcttgc tttttagatt ctatggcatc tttgaacata agtggtcaag gatatggaat
     4561 aaggtataaa tatggtctgt ttgaacaaaa atttgtaaat ggttatcagg tagaagtgcc
     4621 tgataactgg cttacagaag gacgatatgc atgggaaact gtaagaccaa atgaagcaac
     4681 aatggtgaag tttggtggtg aggtagaatt aatcaaggaa ggtagtcatt taaaagtaat
     4741 tcataaaaac taccttcctg taatggcaat gccttatgat ataccaataa tcggatacca
     4801 aaaccaatgt ataaatactt taagattgtt taagagtgaa ataccaaaga gagattttgg
     4861 tgaacttacc tcaaatgctc taaattattc tggtagctat gaagaggctt taaaacataa
     4921 gtattatact gaggaaatat cacaggtatt atatcctgat gattcaaact atgcaggtaa
     4981 gttgttgagg ttgaaacaag aatacttttt tgtaagtgca gggatacaag atataataag
     5041 aaaatataaa aagaacaaat taaatattaa taatttattc gataaggtag caattcatat
     5101 aaatgataca catcctacct tatgcatacc agaacttatg agagttttgc ttgatgaaga
     5161 aaatttatct tgggatgaag catgggaaat aactaaaaaa acagtgtcct atactaatca
     5221 tactataatg tcagaagcaa tggaaaaatg gcctgtaagt atgatgaaag aactattacc
     5281 aagaatttat atgataatag aagaaataaa tagaagatat gtagaggaat taaataataa
     5341 aggatatgac caagatagaa taaaaagaat gagtataata gactgtgaca atatcaatat
     5401 ggcaaactta tgtattgtca caagtcatag tgtaaatgga gttgctaagc ttcatactca
     5461 tattcttgaa acagaggtat taaaagattt ctatcaagat gaaccaaata aatttaataa
     5521 taagactaat ggaattgcac ataggcgatg gcttataagt tcaaatcctc aacttagcaa
     5581 tttaattaca gatttaattg gtgattcatg gaagaaagat acattacaat taaaaaatat
     5641 tgaaaaattt aaaaatgatt cctctgtttt acaaagactt gatgatataa aatacaataa
     5701 taaagcaaat ctagcaaagt ttataaaaga taaatacgat ttaaatgtag acccaagttc
     5761 gatatttgat gttcaagtta agagattgca tgcgtataaa agacagttac tgaatatatt
     5821 taatgtactt catatgtatc atgaattact tgataatcct aatttgaatc ttgacccaag
     5881 aacttttata tttggcgcaa aagcagcacc aggatattat ttggctaaat gtataattaa
     5941 atttataaat tctgttgcaa gtactataaa taatgatgtt agagtaaagg acaagttaaa
     6001 agtagtattt ttagagaatt atggagtatc attagcagaa ataataatac cagcagcaaa
     6061 tgttagtgag caaatttcaa ctacaactaa ggaagcatct ggaacaagta atatgaagtt
     6121 tatgatgaat ggtgctataa ctttggctac acttgacggt gcaaatgtag agatatgtga
     6181 acaggttggt aaagaaaata tgtttttatt tggacttagt gcagaacaag tactaaatta
     6241 taataaatat ggtggatatt cttcacttga cctatatcat tctaatatgg atataaaaag
     6301 ggtagtagat gatttaataa atggatttat tcctaatctt ggcgaagaag gaagaagtat
     6361 ttataattca ctcactactt acaatgatga gtattttgta ttgagagatt ttgaaaatta
     6421 tggtcaagct caagcagata taaacaggtt atatagagat aaagaaaaat ggaataaaat
     6481 gtctttagtt aatatagcta attctggatt tttttcttct gatagaacaa tttctgagta
     6541 tgcaaaagac atttggttta aaagggtgtg attaaatgca aagtgtaata gaatataact
     6601 cttgggataa aaattttaag gctccgtttg gagctttaaa atttgatgag gaattgacca
     6661 tttgtgttaa agtaaatgag ggatacaata tcaagtctat ttccctagaa ataaatagag
     6721 aagaagaaat gagaacaata actttaaatg aagaattaga taatgataaa cttggaaagt
     6781 gcttttgtgg taagattgaa aagtttgatg gtacaggtgt ttatttttat tactttaaag
     6841 ttgatgttga aatggatggt cagataaaga ctttatttta tggaaaaaac agagataatg
     6901 gatattcttg tgaatataat tatagtgata ttaataaata tcaaatcact gtttacaaag
     6961 attttaaagt accaacatgg tacaaagaag gaatacttta tcatatattt gtagatagat
     7021 tcaataatgg aaatcgtagt ggtaaggttg ataatcctaa aaaaaatagt ttcatatatg
     7081 gaaattggga agatacacct atgtacataa aggatagtca aggggatgtt ataagatggg
     7141 atttccatgg tggaaattta agagggataa tcaataaact tggatattta aaaaaattag
     7201 gtgtaagtat actatatcta agccctatat ttgaagcctc tagtaatcat aagtatgata
     7261 caggtgacta taaaaaaatt gacccaatgt ttggggatga agatacattt aaagaactta
     7321 ttgataaagc aaaagaaaaa ggtatatcaa tagtattaga tggagttttt agtcatactg
     7381 gagcagatag taaatatttt aatatgtatg gaaactataa tagtttaggg gcatatcaat
     7441 ctaaagaatc tccatattat tcatggtata tgtttgaaga atttcctcaa aaatataaaa
     7501 gttggtggga tgttaagaca ttgccaaata taaatgaatt agaacatagc tatatggatt
     7561 atataatata tgacaatgat agcgttataa ataaatgggt aaatatgggt attaagggct
     7621 ggagactaga tgttgcagac gaattaccaa ctaagtttat aagagagtta aaaaaggaat
     7681 taaaaaaggc agatgatgac tcaattctta taggtgaagt atgggaagat gcatcaaata
     7741 agattagcta tggtcagaga agaagttatt tattaggtga agaattagat tctgttatgg
     7801 gttatccttt tagaaataac atgttttctt tcttaaaagg agaaattaat tcatatgaac
     7861 tttgtaatag atatatgcag attaaagaaa attatccaaa agaatcattt aggtctaatt
     7921 taaatttaat tggaacacat gatgttacaa gagctaaaac ggaattaaat gatgatgtag
     7981 atttagtgaa gcttgctgta gctatacaga tgacatttga aggagtacca tatatatatt
     8041 atggagatga agcaggtctt tgtggaggtg ttgacccaga taatagaaga acttatccat
     8101 ggaaaaatga agacgaagat atgcttaatt tttatagaga tgttataaga attagaaata
     8161 agaataaact tttaagttct ggaaatacag aatttatata tactaataat gattctgtat
     8221 tttcttttat tagagttaat gaaaataatg atagaatgtt aattttagta aatagaagtg
     8281 aaaatgtgga aagtatatca ttatgtatag agagtagttt tattgaggaa atacctataa
     8341 agtatagtct taaaaatgca aatagtacta tacaaattga aaataatgag ttgaaagtag
     8401 atttagactc taaatcattt agaattttta gagtaaatta aatatctttt tttagaaaaa
     8461 taaatagaaa ataaggtgaa tgtggaaaga gttttgtttt atcaaaactt tttctacttt
     8521 ttttgtataa attatcataa gaattttagt atttcaaaat attacaaaga agtataaaaa
     8581 atatcataat tgtgatatta tataaattag taagataaaa ctgaactctc taccaaagtt
     8641 gtagggagat ttaactttta taatcttaat atggaggaac gtcttatgaa ggtagatcca
     8701 cttacaactc aagtatatga ctatatatca aagaaaatcc aaaatggaga atatgaagct
     8761 aatcagagaa ttactgaatc tgagatttgt aaatctatcg gagttagtag aacaccagcg
     8821 agagaagcat taactaggtt agcaggtgaa aatttactag aaaaaatacc taataaggga
     8881 tttgtagtaa aagaatttca ggaaaaggag aaattagaca cctattccgt aattggagta
     8941 ttggatgcgt tagccggttc ttcagcatta caaaatttaa ctgaatcaga cctagtaaaa
     9001 atggaagaat taacagaaat gatggcggtc tctataaagt ataaaaatta caatagctat
     9061 ttaaaattaa gtaatgaatt tcacgatata tacataacta aatcagataa tcaggtgtta
     9121 gtaaatctat tgaactcttt aagatataat tttatgtcaa agtcatatac tagtgataat
     9181 gaagatgagt tatataaaat gttgacatac agcaataatc aacatattga agtagtaaaa
     9241 tctatgaggg agaatgattt agaaaatgtt gaaagaattt tgagagaaca ttggaaaacg
     9301 atacctatgg aagagatgaa aaaagagaag cttttataaa gtttctcttt attttttata
     9361 ccattttatc actatttttt tgaattatta agtagaaaat aataattaat ttaaacttga
     9421 ggaggaaaac taatatgtat ccaaataaag aaattgcaat agaagagtta gaagtggctg
     9481 gcaaaatgaa tcccggtcca tggataaaac attcttacaa tgttgctaat gtagctaaat
     9541 tgattgcaga gcaatgtgaa aatttgaata gtgaaaaggc ctttgtatgt ggattattac
     9601 atgacattgg aagaagaact ggtatttctt ctgtaaggca tattattgat ggatatgatt
     9661 atgttatttc taagggatgg gatgaagtgg cgagggtatg tttgacacat tcttttcctg
     9721 ttaaggatat agaagctgat attggaaata aagatattag taaagaacag tatgatttta
     9781 tcaatcacta tttaaacaac cttgaatata acgattatga taagttgatt attctttgtg
     9841 atgcattagc agatgcaaat gggttttgta ttttagaaaa aagatttgtt gatacaacac
     9901 gaagatatgg aatatatcca ttttcagttg aacgatggaa taagacttat gagtataaag
     9961 aatatttcga gaaaataatc aacaagtcta tatatacatt gttacccaat attgaaaaat
    10021 ctatatatta ttaaatccta agttaataat ttaatatcca gtatcaccag cagttggtca
    10081 ttatgattga ctgcttttat tatgcttata ttaaaaaaat acactatggt ttgtgtatat
    10141 caaagttata tggaatgaaa agagaattta ttttatcaat taaaaaattt aataaatttt
    10201 atattaaaat taaaatacaa aaataggaaa attatttagt ccaactaaac caagaatttt
    10261 gatatgtgaa ataagaataa tataaaagtt tagtaataaa atacttataa
//
LOCUS       DAMJYX010000112        10281 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJYX010000112 DAMJYX010000000
VERSION     DAMJYX010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.117"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            59..1099
                     /locus_tag="PC479_003000"
     CDS             59..1099
                     /locus_tag="PC479_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805524.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1455..2777
                     /locus_tag="PC479_003001"
     CDS             1455..2777
                     /locus_tag="PC479_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2805525.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3074..4435
                     /locus_tag="PC479_003002"
     CDS             3074..4435
                     /locus_tag="PC479_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2805526.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4508..5902
                     /locus_tag="PC479_003003"
     CDS             4508..5902
                     /locus_tag="PC479_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805527.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            5964..6434
                     /locus_tag="PC479_003004"
     CDS             5964..6434
                     /locus_tag="PC479_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2805528.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6437..8653
                     /locus_tag="PC479_003005"
     CDS             6437..8653
                     /locus_tag="PC479_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2805529.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8736..9407
                     /locus_tag="PC479_003006"
     CDS             8736..9407
                     /locus_tag="PC479_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2805530.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9770..>10281
                     /locus_tag="PC479_003007"
     CDS             9770..>10281
                     /locus_tag="PC479_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDF2805531.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDL"
BASE COUNT         3885 a         1189 c         2060 g         3147 t
ORIGIN      
        1 attaaataga taatgtgaat gattattata tagaatactt atttggagtt gatattttat
       61 ggacaaacac agtgattcag taatgtatgg tggaatggaa gtatataata tttggggaac
      121 aacaacagta gactccataa ttggagagtc tgatgtaatg cgtgctctta agaatagaat
      181 ttgtaaaatt ggaaatagtg attctactgt agctataact ggagaaagtg gcactggaaa
      241 agaattaata gcaagagcca ttcatctcgc aggaaacaga gggaataaaa cattcgttgc
      301 tataaactgt gctgctatac cagaaccttt actagaaagt gaactttttg gatatacaaa
      361 aggagctttt agtggagcag attcaaaagg taaaattggt aagtttgaat tggccaatgg
      421 aggggttgta tttttagatg agataggaga tatgccaatt caactacaat caaagttatt
      481 gagggtatta caggaaaaga agtttacgag aataggttca aatgaactaa ttgatataga
      541 tgttagaatc atatcagcta ctaatagaga tttatttgag ttggttaagg aaaataaatt
      601 tagagaagac ttatactata gattgaatgt aataccttta gaagtgcctc cacttagaga
      661 aagaggagac gatatacaaa tattaataaa caattttata gacaaatatt cgagaaagat
      721 gaagaaaaac gtgtaccata tagagccaga tgtggaacaa atgttgctta agtatccttg
      781 gccaggaaat attagagagt tggaaaatac tatagaactg gcattaaacc ttcttgagga
      841 agatggaata atacataaag gaataataaa tagaaagata cttgaatatt ttaagttaaa
      901 taatataaat ataaagttac ttgaggaaag tgactatgag ataagtatgg aacaagacat
      961 acttacactt gaagagtttg aaagagccta tataaagaaa gtgttaaagt tttacggaaa
     1021 tgatactaag tctaaaaaac tagctgccaa aaaacttgga atatcactag ctacattgta
     1081 cagaaaaata gatgtataat aattaggctt tttattctca attctaaaat ttgaaattat
     1141 aaaattgata aaaaacactt ggaaaactat tttctaagtg tttttttgtg taatagcatt
     1201 ttattatcca aaaataaaaa aaatttaaaa gaatttaaaa aaataaatgc tttaataaat
     1261 tatcaaatac taacaaatat aataaaaatt atatatgtaa aacaattatg ttaaaaaatt
     1321 ggcattataa ttgcttcata ttaagttgag atgacaaaat aaaaaaagaa aggatgttgt
     1381 gtgagataat aataactgtc attaaaaaat agttgggagt gataaccttg agtaaatatc
     1441 aaaagaatgt ttctatgttt gatattgatg gaaggccacc aattttaaag tgtttgccac
     1501 tatcaatgca acatatacta gcaatgatag ttggaactgt tacagtacct atagtcgtag
     1561 gaggagctgt gggtgttagc cctacacaaa taacactttt agtgcaatat gcattaataa
     1621 tagctggtat atcaacactt atacaactat atccaatagg aaatattggt tcaagattgc
     1681 ctataatttt tggagttggt tttacttatg taccaacact tgtagcagtc ggaggaaatt
     1741 atggattagc aagcattttg ggtgcacaat taataggggg agtggctaca gtattaatag
     1801 ggatattcat aaaaaagata agaaaatttt ttccgaatat agttgcagga acagtggttt
     1861 ttacaattgg attatctttg tatcctatag caataaatta tatggcaggt ggtgtaaata
     1921 gtcctactta tgcctctatg caaaactggg cagtagcggc tataacatta gtggtagtta
     1981 taggtcttag tcaatttgca aaaggatatg caaaattggc agcaatattt ttgggaatta
     2041 tagttggata ttgtgtatct ttagttctgg gtataataaa ttttgaccct attagagagg
     2101 caacttggtt tgcgatacct aaaccgcttg aatttgggat ggaatttgat atgcctgtaa
     2161 taatctcagt agtaattgta agtataatca atgctgttca agcaattgga gatttatcag
     2221 ctactacaat gggaggcatg gataggaata tcacagataa ggaattatca agtggagtaa
     2281 taggaagtgg aatttgtact atgataggag ctttgtttgg aggtttacca ccatcatcat
     2341 atagccaaaa cgtggggatg gtagctatga acaaagtaat aagtagattt gtgcttggaa
     2401 tagctggtat atttatgtta ttgtcggggt ttgtgcctaa atttggtgca ctaatgacta
     2461 ctataccata ttctgtttta ggaggagcta caatatctat atttagtatg ataacaatga
     2521 caggtataaa gttaattatt acagaagagt tgtcatcaag aaatattacc attgttggtt
     2581 tggcaattgc cttaggcatg ggaataacat cagtaccagc agcccaagag gcatttcctt
     2641 catttgtaaa gttagtattt ggagaatctc caatagtaat tgcaacattg gttgcattcg
     2701 ttttaaatct aataatacca aataaaagtc tagaggatga ggctaaagaa agagctgtta
     2761 tggaagagac taattaagat ataataattt aaaagaattg tttaaattat ttagtatgag
     2821 attgttagat tatttttgat agctattcat tttaataaaa aatttaacaa ataaatatat
     2881 accaggcaat taatgttcat ttaagttgaa aatcggggag ggaatattta gaaatttgtt
     2941 aattttttat aaaaatagct cagtttagct gtgtttatcg gttgagcaat aaattcatgg
     3001 tcataaaaat atttatagaa aataattata aaaatattta gtcaagatat ttatgtatgg
     3061 gaggatttat tttatgaaaa cacgtaatac ttcaaaatat gatgtcgatg ggataccacc
     3121 actaaaggaa tcaatacctt tggccttaca acatctgtta gcaatgatag taggaaatat
     3181 ggtacctgcg atattaatag caaacgtggt aggacttaac caggggcaag caacaatgtt
     3241 gattcagggt tctatgttag cagcaggttt agcaacgttt ttacagttat atccaatacc
     3301 tttatttaaa ggatttaagt taggttcaag attacctgta atgatgggaa tgagttatgt
     3361 atttttagga gcttgtcttt cagtagcagc taaagatggt ttagcagctc tatttggagc
     3421 acagattgct gctggggtaa tcgtattctt tgtaggattt ggagttaaga aaattagaca
     3481 tatattcact ccaatagttt ctggaactat aatagcatgt atgggacttg gactattcgc
     3541 aacagctata aagaatttag ctggaggaga aggcacacaa acatttggaa gcccaataaa
     3601 cttcacagta ggagttatag ttgcttttgt aataataatg ataaataaat acggtaaagg
     3661 tttagtaaaa aattcttcaa tattaatagg aatattagta ggttatgcta tatctttagt
     3721 tttaggattg gttgatttct cagccgttca aggagcagct atagtatctt taccaacacc
     3781 agcagctttt ggattagaat ttagaccaga gttaatagta atgtttacta ttatttacat
     3841 aataggtata gctgatatga tgggtgcctg tacaatagct acagtaggag caatggatag
     3901 agaggtaact gacgaagaat tagcatcagt tgttttagga aattctataa cttctataat
     3961 ttcatcacta tttgcagctc ttccaactgg agtatttagc caaaatacag taattgtatc
     4021 tatgaacaaa gttacaagta gatttgtaat agcactaggg gcacttgtat tattactagc
     4081 agggatatct ccagcattgg gtgcaataat gacaactata ccatcttgtg ttgttggtgg
     4141 agcaacttta gtagttttct catcaatagc aatgtctggt ttctctatta tgagtatgga
     4201 tggattcact gaagaaaaca atcttatagc aggtgtatct atagcaacaa gtatgggtct
     4261 tacaactgct ccacaagttt tagaccaatt cccagaaaca attggaactg tccttggagg
     4321 gtcaagtata gtttctggag caataatagc tatattactt caaacattat ttaaattaaa
     4381 gtcacgtaaa agtgctgaaa acgttacttc aaatcttgag gaaaatattg gataaatgta
     4441 ttagatataa tatgagaaaa ccatatggta aaatgttgta atgaaagtat tcaaaggggg
     4501 atttaagatg attgatatac taataaaaaa tgcaataatt gttacagtta ataaagacag
     4561 agatgttata tttgatggag caatagctat tcaaggtgac agaatcttgg acataggaaa
     4621 tacagttgat attgagccta agtatcttga tgctaaaaaa gttatagatg ctaatggaaa
     4681 agtaatattc cctggcttta taaatactca taatcacctg ttccaagtgt tattaaaagg
     4741 tttaggtgat gatatggcac ttcatgagtg gttaaataca atgatgtttc catcagcaaa
     4801 attcttaaca gaacaagata cttatgatgc tgcaatgctt ggatgtatgg aaggtttaaa
     4861 aagtggtata actacaatgg ttgattatat gcatactcac aatagaccag gacttacaga
     4921 tggaatagta aaggcatata aagatttagg tataagaggg gttgtaggta gaggttgtat
     4981 agatttaggt attcacaaag agcttataga agatgtggaa acggttgaaa aagatttgag
     5041 aagggtattc gaaaaatatc ataattcaga aaatggaaga ataaaaatct gtgtagctcc
     5101 ttcttcaatg tgggctatat ctgaagaaat gggtaagatg ctttggaata tagttaaaga
     5161 atatgattca tattttacag ttcatatatc tgaaacagaa tttgcaagaa cagcaacaaa
     5221 ggatatacat ggagaaatag atataaaatt acttgaaaaa tggggtatag taggtccaga
     5281 agttgtagca gtacattgtg tatgtataac tgatgaagac atggaaatgc ttaaaaagta
     5341 tgacataaaa gtttctcata atgtagcaag taacatgtat cttgcttctg gtgttgcacc
     5401 tgtgccagaa atgttaaaaa aaggtataaa tgtgagttta ggtcttgacg gtgcagcaag
     5461 taataacgca caagatatgg tagagcttat gaaattaaca gctttacaac acaaagtaaa
     5521 taattgtgac cctctggcaa tttctgcaga gaaagttctt gaaatggcta caattgaggg
     5581 agcaagaact cttagaatgg aagatgaaat aggttctttg gagattggga aaaaagctga
     5641 cctagtaata tttaactcaa tgctttctcc aaaatcaata cctcttcaca atcctgtatc
     5701 aactttggta tactcagcta gtatgcacaa tgtagagagt gttttagttg atggaaatgt
     5761 aatacttgaa aatggaaaag taactacaat tgaaagtgaa gaacaagtgt tgcttaatgc
     5821 acaacatgca gcagaagaat tatgtaagag agctaatgta actaatagaa tggaaggaca
     5881 taagtggaat agcttatact aatattaaaa taagattgta ataatattct attacaacat
     5941 tagtttatct gtaggaggag aaaatggaaa aaataaaatt aaatattaat gattacatgt
     6001 atgaaataga agttgaaaag aatgaaactc ttttgcatgt acttcgtgaa aaattaaatc
     6061 taactggagc gaaacaaggt tgtaatagtg gcagttgtgg agcatgtaaa gttttgatag
     6121 atggagttga catgaaatct tgtaaacttc ttgcaaaaaa ctgcgaaggt aaagttatat
     6181 acactataga aagttttgct aatggagaaa atcttcatcc aatacaagaa gcttttatag
     6241 aagtcggagc tgtacaatgt ggatattgta ctcctggtat gattataaca acacaagctc
     6301 ttttaaatag aaatgcatct cctaatgaag atgaaattag agaagctttt aaagaaaatc
     6361 tctgtagatg cacaggatat gtaaagatta tagaagctgt aaaattatct gcagctagat
     6421 tagggggtag ataattatgg aaaaggtaat aggaaaatca taccctctaa aagatgcagc
     6481 tttaaaagtt actggtcaaa tgaaatacgt ggctgatatg aaaccacaaa atgtacttta
     6541 cgctaagatg cttttaagcc ctgtagcaca tgcaaaaata aaaagtatag atacaagtga
     6601 agctgagaaa gtagtaggtg ttagagctat agctacttat ctcaatacat cacaaagaaa
     6661 atacaatggt tcattaagat tttatgagca caaaatacct gaaaatgaag ttatattttc
     6721 agatacagtt agatttgttg gtgaccgtgt tgcagcagta gctgcagaga ctccagaaat
     6781 agcagagaaa gctgtaaaac ttataaaagt tgaatatgaa gaattgccag ctgtatttac
     6841 tatacaagat gcaataaaag aagatgctat ctgcatacat ggagattcaa atatagtttc
     6901 tcaaaacaac ataaatgggg gagatgtaga aaaaggcttc aaggaatgtg attatatatt
     6961 tgaagatgaa tactctactc cagcaataca tcatagtgca atagagactc atagtgtaat
     7021 tgcacactat gattataatg ataaattaac tgtacataca ccttgccaaa atacatttgg
     7081 atttagaata atactatctc aaatttttgg gatgtcatta aataaagtaa gagttataag
     7141 acctgctatt ggaggctcat ttggtggaaa attagaactt acaattgaac cagtagcagc
     7201 cgcattatca aaaatgacaa gaagaccagt taaaatggtt cttacaagaa aggaatctat
     7261 gatatcaaca agaactcgtc atgggtcttt tgtaaaatta aaaactggtg taaataaaga
     7321 tggtgaagta atagcacaag acataaaaat atatacaaat acaggagcat atgcatcaag
     7381 tgctttaaat gttataggag cacttagtca taaagtattt aaagtttata aaataccaaa
     7441 tataaagttt actgggatgc ctgtatatac aaatacacca atagcaggag ctatgagagg
     7501 ctatgggtca cctcaaatat ttatggctca acaagctcaa tttgcgaaaa tagcaaagga
     7561 aataggtatg gatttagtcg attttcaaaa taaaaatgca gtagagccag atgatgtcga
     7621 tataatcttc cacggttctc ttggaaatcc tagagtattg gattgtatag agcaaggtaa
     7681 gaagatgttt aagtgggatg aaaagaaaaa acttccaaaa gaagaaggaa aatatttaag
     7741 aggtataggt atggcaatag gtgcacatgg taatggagtt tttggtgcac atagagatgt
     7801 tgttacattg actcttaaat taaatgaaga tggtacgttg acactactaa caggagctca
     7861 tgatatgggt aatggtgttg taactatgca aacaatgatg gtagctgaag ttttaggaat
     7921 aacacctgat aaagttgata catttgaaac tgatacggat gcatgtacat tcaaccttgg
     7981 tgactatgca agtagagggg tatttgtaga aggtggagga gctaaaaaga cagcagagaa
     8041 attgaaaaat cttatattag aagaagcaga aaaattactt gaaacatcaa aagaagattt
     8101 atatctagac aatggatgtg taattagtaa acttgatgaa aatgtaaaag catcactttc
     8161 tgatgtcgca gtatatagtc aaagtaaatc attaagagag ttaactgttg ttgaagatta
     8221 ttcttcacca gctggacgta cttcttatgg tgtacatttt gctgaagttt tagtagataa
     8281 agaaactaag gatataaaag tagttgattt tgtagctgta catgatgttg gtagagttat
     8341 aaatcctcta aatttagaag gacaattaga aggtgggatt catatgggtc taggatacgc
     8401 attgagtgaa gaattggcat ttgatgaaca aggtagacct aaagctactg attttaaaac
     8461 atataaatta tttagaactg tagatatgcc aaaatgtcaa gttgcatttg tagatggtta
     8521 tgaaaaagct gggccttttg gtggtaagag tattggagaa tgtgccgttg taccagttgc
     8581 gccagctgtt gcaaatgcag tatataatgc aactgatgta gagatacatt cattaccaat
     8641 aaaattaaaa tagtagaaat taaagcagaa ataacaattt attgtatttg taaatagata
     8701 ataaaaatag atttatttgg tgaggagata ttattatggc aaagatatta ggtatttgtg
     8761 gaagtccacg taaaggagca acagaatatg cagtgttaga agctttgaaa gaagcagaaa
     8821 ctatacctgg tatagaaact gagtattggt cagttagagg gaaaaaaata agtccttgtg
     8881 tacattgtga tgcatgtata agaaaagaaa ctatgtgtat tataaaagat gatatccaag
     8941 aattagagca aaaaatattg gaggcagatg gaattatagt aggttctcca gtttatgata
     9001 tgaatataac tgctcaactt acagcagtat ttaatagact tcgtccaatg tatttagttc
     9061 atccaggaaa acttcaaaat aaggttggtg cagctataac tactggtgga actagatatg
     9121 gtggccaaga attgacaaaa cttcctataa taaatttctt cctaatgcat gaaatgttag
     9181 tgtctggagg tttaggtgga tgctatatag gtggtacaat atggagtaaa gatggaaaag
     9241 caaaaggtgc ccaagaggat gagacaggta tggacacagt aaaaagactt ggaaaaggtg
     9301 cagcagaggc agttatggtt tctaagtttg gtcttgaaaa atggaatgtc gtaaaagaag
     9361 aattagatgt taaaaaagat gaaaaatcac ctcttagaga ccactaaaga tttattatat
     9421 tattgaatat atacaaatag tgatgtaata gattattgca tcactatttt atgttttaat
     9481 ttagatttta taaattttat attaaaaaat atagaaacaa agtgttttga gatttatttt
     9541 taaattttaa aaatgagaat taaaaagtga gaagatagaa aaggtttttt agttattaaa
     9601 ttctacatac attgtatgaa ctaaagatag aaaagaaaaa ataataaaag cattgcaatt
     9661 tctatactaa agagtcatgg aaaaatatct agtatatatt tagtattggc atgttaattg
     9721 cttatatatt aaagtataaa aatttaacaa taatttggag gtattttaga tgttaaaaga
     9781 aattaaatgg aaagtaaata atttgccaaa gggcgataag gaaaattgta tcaaattttt
     9841 aaatgaagaa gaaataacta aggttagaaa ttttcacaaa agctttcctc aatataaaga
     9901 aactccatta gcaaacctag aaggtttagc aaaaaaatta ggtgttgcag gagtatatgt
     9961 aaaagatgaa tcttatagat ttggattaaa tgcatttaaa gtattaggtg gttcttattc
    10021 tatgggtaga tacttagctc aaagattaga tacagatata tctgagttag ggtatgacaa
    10081 attgacttcg aaagaaataa aagaaaaatt aggagaaata actttcttta cagctacaga
    10141 tggaaatcac ggtagaggag ttgcttggac agctaataaa ttaggtcaaa aatctgttgt
    10201 attaatgcca aaaggttctt ctgaatttag attaaataaa attaaaggag aaggtgcaga
    10261 tgctagtata acagacttaa a
//
LOCUS       DAMJYX010000113        10125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJYX010000113 DAMJYX010000000
VERSION     DAMJYX010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.118"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(80..1960)
                     /locus_tag="PC479_003008"
     CDS             complement(80..1960)
                     /locus_tag="PC479_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2805532.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2170..8133)
                     /locus_tag="PC479_003009"
     CDS             complement(2170..8133)
                     /locus_tag="PC479_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2805533.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8257..8342)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8514..9917)
                     /locus_tag="PC479_003010"
     CDS             complement(8514..9917)
                     /locus_tag="PC479_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2805534.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2779 a         1884 c         1151 g         4311 t
ORIGIN      
        1 taatgaaaat aaaataagaa tgcatttaaa tatataactt tatatctaaa tacattctta
       61 ttttctattt acttaatatt tatttttcaa ttaaactctt aatcttgtct attacagatg
      121 aggctattcc tccacctact tcaaacaact ttgatgaatt tttacctttt aatgacgatt
      181 cttgtgattt atctaacgta tttgtagcaa gtataactgg tgaattgttt ttagctgcta
      241 atggtccaac tgctaaagca tctataagtt tatcttgatt tgcaactaca actccatcta
      301 gcttagattg agagtagaat ttatctatta ctttggcatt cgtctcttgt ctagtttgac
      361 ctgccaccct attgtttgat atatctttgt ttgctatttt tccaacttgt tcaatagcct
      421 tgttagatat tttttctact ccaccaataa aatatatatt tgacacctta tttgatttaa
      481 taaattgttc agcttcatta tctacaccat ctttttgtgt aagtactatt ggagtttttt
      541 ctttaccagc cagtgatgct attgataatg aatcagcttc tccatttcct gctcctatat
      601 acaacttgct tacattattc gtcttatcta tttcttttgc tatattaata gaagtttgat
      661 atcttgtatc tccacctatt ctttgtactg atatttttga atttacagct ttgatagctt
      721 caacaactga atttggcatt gagttgtttc cacctatcac aactactttt gatggattta
      781 gccttttcaa ttcttctaca gttttttgag gaagtttatc atttgatgat aaaagaatcg
      841 gtgagttttt tacactagca agtggtgtag ctgtaagccc atccaccata cttttatctt
      901 ctccatttac tattacaact gtatctgatt ttgtccaacc actttgagat attttgatag
      961 ctgtctcaaa tctatcatca ccttttaatt ttttgactgt gacattagat gtagaatcac
     1021 ttgaatttcc tcctccagag cttccaccac tagatgagcc gcctgaatta cttcctccac
     1081 cattatttcc accactacca ttatctggtg gtaattcatc cggttttgat aattcatttt
     1141 caactatgta ttctaaactt tttggaagtg taacttcaac atatttgttt attgtaactt
     1201 ttcttccctt agttgttttt tgtgctaatc caagttttaa tattacattt gaaccacttt
     1261 cttgtgtttc tttactaaca atagaatagt ttatcctaat attgtttaat aaatattcaa
     1321 gtgcttcact tttctctacc ttatactttt cagggtcatt tttactagaa ccagttcccc
     1381 caatcggttt tacttcactc tcattaataa gggtaaatat taatccttca tcagagctta
     1441 tattttcata atccactgtt ttgttgttat atgttacaat ttttaaagct ggtttatcat
     1501 ttgttccttt tcctaaaact ttatagtcag catttgagtt acttccagat ttttttacag
     1561 atattcttcc tatattcaat ctatttccgt ctagtaagtc ttcatttgat gtaacatata
     1621 tatctaatgt agttgtatta cttgatttgt tgtattctgc tctacaagta gcatttgcat
     1681 ttccactttt tatctgagat tttatattat cagaccaagt aatcttttct ttctgctcat
     1741 tatctactac ttcaacctca ccttgtactt ttatactggc ttgaaatgaa tatactatgt
     1801 tttctactga gtccacagat atatctatag tatttacttt ctcatacgtt tttgttcctt
     1861 gtaaaactgg agctgcatat gcacatgtag cactaccagt tacagctact gccatagttg
     1921 atactgctat agttcttata aaagattttc ttttactcat taacacccgt ccttatctat
     1981 cattaactaa gtggataaat ttacttatcc acctagcttt tatatttaat ttataaatta
     2041 ccctaaattt atgattacat ttatatactt tacttttaat atctactatt ttaaaactat
     2101 tctagtacct aatcttatta gatatacaat gttagtgtta gtatttttac gtttttagag
     2161 ttccacctac tatttatctc taacaatatt accctttact ttattgctta cagttataac
     2221 cttcttatta ctgatatcaa tagaatttct tgtagctttt ctagctttac tattactatc
     2281 agtaagactt attgtcttta actcatcata attcttagct cctacaggta catacattgt
     2341 atcacttaca aatctttgac ctactggagt atctccttga aggtcattgt atctatatag
     2401 ttcagcctta accttatcac ttaaagaatc aaagttttct tttggcatat agtaaaccca
     2461 tgtaccttta ctgatttcac ctagttcacc tttttctgga acttctgcaa ataaaagtgc
     2521 ttctccagat atagtcttat tgtttttatc tataagtagt ggtaaattaa atgctggatt
     2581 tccactgtat tcacctgtaa taactattga acccgctgga atcttacctg tttcatcttc
     2641 acttgtgcta gagtcatcac tttgatatac ataatcttct gctaattttc caattccatt
     2701 agtacgagtt tcgtctgcat tattcattgt gccaatttct atattatcac ctggaggtcc
     2761 tattatatct aattctgcaa cttctatatt agacttatta tttgagatta gttttacata
     2821 tctagcttgg tatgtccaaa gtttaccatc tttattaaat gtaactctag cttcattatc
     2881 ttggtctttt cctccaccaa ctaaatcttg tccatattca aaagttcctt gacttacatc
     2941 ttcccagttc tttttatcat tacttacttg tattttgtaa ttagttaaag gattgtatgt
     3001 tgtgctagta ttctttctaa agttaaataa tccttttaat gagaacttcc tagcttctgt
     3061 ctctggtttt gtatatttta gtcctactat aggtaatgtt tctttcatgt ctactattat
     3121 atatggattt tcctctacat ttatatcagg gtctttttga gggtctttgt tccactctgc
     3181 tttacttatt atctttcctt tatatgtagt atttgcatca ttatctttta tattatcaat
     3241 ttcaggattt tgtaatggtc catgtgagtc attttcagtt ctaacatcac cgtcagaaac
     3301 agtatttgtt atgaatgacc atgctgattt atttattttc ccttcatctg ataattttac
     3361 agtaccaact tcaaattctt ctgttggatt taggtgataa tcatatgcaa ctaccttata
     3421 tgtaaatgct ctgttattaa gaggagatat attatcagta aatttagtct tactatttcc
     3481 ttttgcatcc acaaatccaa ctgttctgta ttttacctgt gttccatttt caccatctaa
     3541 ataatttcca tcacttctta ttatttcgta tcctagtatc ttatctttat ccttatctac
     3601 atctaggtct atagatattt tattagattt aactagagaa ccactttgta ttccatcaga
     3661 aaaactagca cttactttag tatcttttgc catactagct atattttcat ttaaaatctt
     3721 tcttctagct tcgtcattta catattgtat ctttcttgat tctttttcgt agttcttact
     3781 atccattata ctgtttaatt ctggtgtaat ttctactccc catgccttaa agtaatctct
     3841 taaatctttt tcaacaacac ttgatgccat tgctactagc atttggtctt ttggcaaact
     3901 attaattggg tcactacttg aaagtgctct gtattttctt gaaatctttg catagaatgt
     3961 atcattactt aaatcagcat ctgagttatt ctttaacatt gaacccgtaa aatcatcatc
     4021 ataagctaga tgtaattgcc ataacattcc aagcctttca aatacattgc ttggtatacc
     4081 taagctattt gaagttacat gctcatatat cttatccatt gttgaacctt caattcttga
     4141 atgtgtctta tcatcaaagg tctgagcaag taaagcaagt acattgttac tagtttcgct
     4201 atatgtcatc tttccaatat ctgtaacatg tcctatctca tgacctattc cccatccaaa
     4261 taagtttcct cctgtagctt ttccactatc atctatttgg aaaggtactc cattcataag
     4321 ccctggaact gaaccatatt ctactcctac atgtaatcca gaagcataca taaatgctcc
     4381 aataaacatt ctttggtatt ttatgttcat acgagatttt ggagccatat catttttagt
     4441 taagttaaat tcattatcat ctacagttcc attaccatct aaatcttgtg ttttaaatac
     4501 tcctctttct gagtaaacta aatccattat ttgttcccat gcaagcatac tattatataa
     4561 tctatctact tgtgtattta aatcagcatt tccttttgat atcccttgat atacagctgt
     4621 tgcaggaaca tttaatgtta ctttatcact ttcaatatct gtagtgttta acacaccagt
     4681 tttttctgaa tatccatatg aattaccaac atttgttcct ggataatatg gttctccttt
     4741 tacagcatct ttatatctag ctaattcttc tatatatgct tttatctttt tcttagattc
     4801 agaaactccc gcaggactct tatcatttat tgttccattt agattaaggt gtggtatctt
     4861 agtagctcct gctacacgta cttttatatc tcctccactt ggagtatctt cagcgtatct
     4921 tatgtataaa cttcctccac attctatatc taaactatga agtttgctta attgaattac
     4981 attttttcca gtgttaagat ttatttctcc actcttatat gcaccagatt ctccatagta
     5041 ctgtgtatat atcaacttag gagtttttcc acttttactt ccaacatata ctgtgatttc
     5101 atctcctgct ctagcagcta ctcctaatgc ctgccagtca ttttgcatac ctaaattatt
     5161 ggaatttaca gtatactgtc cgtttttatc tttatatttt cctaatttat ttatttcttg
     5221 atttacagta gttacatcag taagtgtggc tctatcatta tataagtctt gagctaattt
     5281 taattctctc tcaataactg tcttatatgg atgatattct ccactagctt tatctggagt
     5341 atttaatcta tcttttaatt cagttatttt ttcttgagtt acatcttctt ttaattcaac
     5401 ttgtaggtca tccataaata gatttcttac atcatcttct atactgtcat actcataaaa
     5461 cttaagttct gatatattag acttatatga ataggatgaa caaacactta cttgtatctt
     5521 ggtagcagtt attggttctt ttaatctaac cattacatac ttattattat ttacagggtc
     5581 tgtctttgtt tcaaaaggaa catcccccag attgtgtaat tgaccatttt catcccaata
     5641 tcgtacctca aagtcattta ttgtcttatc agaaacatca aaacgaggtg tagccataaa
     5701 agtatccata gtataagatt tatcaaaagt tattattggt cctctattcg ttccctcagc
     5761 tgcattatat gttgggaatg tccaatgtgt tgtaaagtcc ccatctacta cattgaattt
     5821 atcaaatgga tttttatcac tatattcact tttgtcatat cctgctggat aagtaacatc
     5881 tacaatgtga tttgttggta ttccaaatcc accattctct ttaggagtat taattaatct
     5941 atagttaggt gttgttggtg catccatgct ttcagttgta cctatatatg ttttagacat
     6001 tccactagtt cccaagtggt tagtagctgt cattctaact tcatatgttg tattatcctt
     6061 aagaccttct atctttgaac ttgtcccacg aataagctta tcattgtatt ttatccaaga
     6121 cccattagag cctttttctt tatagtacaa gtcaaaatct ttagcttgtt tatgattttt
     6181 ccagcttaca tcaagagatt taaatttacc tttaacattt ataccttctg gtggttctgg
     6241 ttttgtgtct ggaacaactt gaaattctaa tatactatct gggctaaatt gtataggttt
     6301 atagtttgaa tctacatttt ctggtactcc gtctaagttt tctggtgttg catctttata
     6361 accactagac caatctccat ttaaagattg tatgctaacc ctatatagtc catatgcttc
     6421 tatatctcta acttctattg tatttttact ggtttgtaat tttttagtag ataatacttt
     6481 tcccttacta tcaattttat ctagtataag ttcgtatgct gatacattta cttcattatt
     6541 ccaataaaaa gatacttttt catttccaac agcagtgcta gtcgatattt tatttatttt
     6601 aggtatattc atctctggct ttggtatttc tttatataca ttgtttaaaa attcaacttt
     6661 tgatatttct gcaagatttc tatcgtttgc tgttgtagct gttattttta tagttatctt
     6721 ttttactgct acttgtttac caagatttat tactatatca ctattttgaa catctgcata
     6781 tgtttctaat gttgacttac tatatgtaga tgttgtagag ttatcatctt tactgatatt
     6841 accctcttct tttggagtat cttcatctga attaggttta tccattgatg aattttcttt
     6901 atcattattt ttatcttgat tggtattaga gtttacttct tgtttatcgc tattttcctg
     6961 ttgttcatca ttattttcat ctttattatt tgagttatta tcagtctggt ttatattttc
     7021 atctttatta tttacttctt cttgatttac ttcttcttgt tcgttattta cttcctcttg
     7081 ttgctcctca tcttttaagt ttttcatata ttctttgttt tcataaggaa tctgttgaac
     7141 attaccatct ttgtcagtaa cctcaactat tccttttgct ggagcagtac cttctggagc
     7201 ttttatattg atgacttcca tattaagtgc ttctgataaa gctatatcca ttactgctgg
     7261 tttttcctca gatatttcat cattttttga agatattgta actccattat tttctccact
     7321 aaataaattt tctacattac cattaatatc tacatcacta tttcctttta attcaacctt
     7381 acttgtatct actataggat tggtagatac tatactttct aggctttttg catcttcact
     7441 ttgtcctaaa tttttctgta cataatctaa gtcaacaata tctacctttc catctctatt
     7501 taaatcaaat tctcttttat ttgaatctat attctctaaa accttattat aatcactctt
     7561 atctacttta ccgtctccat ttacatcacc cattaagaac aattcataat ttaaaggttt
     7621 atttttatta tctacacttg ctctattgct taataagact ctttgtgaat aacttgatat
     7681 atcaatgtct ttaatttctt tgttcttaaa tccatctcca gagacttcta cattgtattt
     7741 tccaacaggt aaattattaa atacaatatt ataatatgat atattctcat ctttattttt
     7801 tatttcactt ctacttgcag ataatttttt tatagtatat tctatattat ctgtcccaac
     7861 atttactgtt cctgaatctt tttctccaag ctttattatt ccactattgt tactatcatc
     7921 ttttaatcgt aactgcatat tagtcatact ttctgaatcg ctatttttta ttggcagtga
     7981 aaaatttaaa tctacttcaa gactccccct taacttttct gtaactgatt tttcaactgc
     8041 ccttagcttc ccctctaata taggttctgc aaaaacgttt agtggtgcac tacacataga
     8101 tactgctagt acacaagata ttgccttttt catgaaataa caatcctccc ttaataaata
     8161 tctaattttt catgaattta aatactataa gtttcagaca ataaaaaaag acactcaaag
     8221 agaatcttaa ttataaaata aaaagacttt actcttttta gcaactggct gatatggctt
     8281 taaaagcttt aatccctata gttttgcgtc attagatttc cctaattttg ccatttaatt
     8341 ttcactactt tcttacccat tttatactat atttaaactt tttaatacaa tttttttaga
     8401 ttaaattata aaaaaataac atttatatta atttattaat ataaatgtta ttttccatta
     8461 taatttaatt atttatatag cttccttaca agcataattt tgtgtatatt ttcttattct
     8521 ttatatcttc cataaaattc atgtgtagca tatagattct tatctcctgt tatagctata
     8581 ccaaatccca ttatttcata ccctttatgc aacatgttag ttccttcttc tggatttgtc
     8641 ttccactcat taaaaataaa gtttcctata tcatctgcat ctctatttgt atatacaggt
     8701 ttatctggaa ttttatattt aattatattt tcaccttgaa ctgctatata tccaggttta
     8761 atctcactcc aatctaagta tttcaaaaaa gtagaatatg aattttttcc atttatcgta
     8821 tgagataaag tcttcttatt tgccattaaa ttagaccaat catttgctaa accctctaat
     8881 ctgcttgcta cagataacgc tcttactcca ttctcttttc tatactgctt tgtaagagca
     8941 tatactttac cctttattct ctctttaaat gctctatcat ttggattgta atataaacct
     9001 tgttgatttg gtgaagcttg atttatacat tgcttgataa cattctgatt tatgcctcct
     9061 agtacagtca atttgtctgc accacttaaa atactcttgt tactgccatt ttcaactaaa
     9121 actacaggag cattttttgc tattgtagag gcagccaaag cgtctgttaa ttgtaagcct
     9181 ttacttagat aaaattcttt actattttta taaaactctt gtataactat cttattagtt
     9241 tcaaatctat ctgtgccacc caatctaata gaatttgtgc tcttaactaa ttcgtcgctg
     9301 attatttcac tagaaccaag cgcataactc tttaagcctg tcgtgtcaaa tcccacacta
     9361 tttccatctg taagtattat aggtactcca tctctagcag ctactggtga tgcacttatc
     9421 gcatctgctt ctcctgtaaa tccattggct ataattactt tatctattgg cttaatttct
     9481 ttgatttttt cagctatttt tatacttgta tcaaatctat tttcaccaaa gattctttgt
     9541 acaaccttct ttttggatag taactgattc tcaacatttt tacttattgt tgattctgag
     9601 cctattatat acacagtctt tgctttgttt aatctttcca tagttgtgtt tggaatctga
     9661 ttttgttttg tcatcaaaat aggcgcattt aaggctccag ctaaaccact tgcacttaga
     9721 ccatctgcta agctattatc tgagttgatt agtattgctg tattgtagtt ttgcttatct
     9781 gctatttttg ctgctgtctc atatctgtca ttccctttta tttgctcaac tgatgtcaac
     9841 gcatttacca taggagtatt taccattact aaaaatagtg taagtcctat agctaatatc
     9901 tttttactat tcttcatatt attttctacc cccaatgtac ttttcatatt ttttattagt
     9961 actcatactt atttttaatc aaaagttaca attttgttac catattttat atatgatacc
    10021 atagttatcc atacaactca aacaatatac ctaaagtttt tattctctat aaattcaaaa
    10081 cattataatt tattttattt aattaagtta taagttcaaa ataat
//
LOCUS       DAMJYX010000114         9833 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJYX010000114 DAMJYX010000000
VERSION     DAMJYX010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.119"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            178..1011
                     /locus_tag="PC479_003011"
     CDS             178..1011
                     /locus_tag="PC479_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2805535.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRVPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1104..2036
                     /locus_tag="PC479_003012"
     CDS             1104..2036
                     /locus_tag="PC479_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2805536.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2088..2570
                     /locus_tag="PC479_003013"
     CDS             2088..2570
                     /locus_tag="PC479_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2805537.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2582..3451
                     /locus_tag="PC479_003014"
     CDS             2582..3451
                     /locus_tag="PC479_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2805538.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3494..4249
                     /locus_tag="PC479_003015"
     CDS             3494..4249
                     /locus_tag="PC479_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2805539.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4291..5112
                     /locus_tag="PC479_003016"
     CDS             4291..5112
                     /locus_tag="PC479_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2805540.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5117..5878
                     /locus_tag="PC479_003017"
     CDS             5117..5878
                     /locus_tag="PC479_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2805541.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6039..6296
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6409..6648
                     /locus_tag="PC479_003018"
     CDS             6409..6648
                     /locus_tag="PC479_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2805542.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            6952..7947
                     /gene="ilvC"
                     /locus_tag="PC479_003019"
     CDS             6952..7947
                     /gene="ilvC"
                     /locus_tag="PC479_003019"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2805543.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDVAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8000..9688
                     /gene="ilvB"
                     /locus_tag="PC479_003020"
     CDS             8000..9688
                     /gene="ilvB"
                     /locus_tag="PC479_003020"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2805544.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
BASE COUNT         4190 a          943 c         1766 g         2934 t
ORIGIN      
        1 attctataca attatacaga aattatacag cataataatc accgaaataa caaattttaa
       61 aaattaagca aaactttttg tcgaagttca acgtctatat atatgtagtg taaatttttg
      121 ggataaaata ctacaaaaat ataaatgtaa aaatatagta atattggagg tcgaacgatg
      181 agaaataaaa agaaaaaaat aaatagaaag aagttgtatt tgcttttaag tgctattgta
      241 gtgtgtctgg cttttatagt agttggtgtt cgcgtcagct ctttaaatat gaaggaaaat
      301 gaaattagta gaaatgcaaa actgtcaagt tctactatta cagatatagt atctaataca
      361 tcaatagaag ttagtaaagg gccaagtaaa agttctggaa aaatagcata cataactata
      421 gatgatggtc catctaaatt tactgaccag atgataaaaa ctttaaataa atacaatgtt
      481 aaagcaactt tttttatgat agatgggaat atgaaggaat atccacagca agttaaaaat
      541 ataattaaaa atgggaacac agcaggtttt catagtgtat cacatgatat tcacaagctt
      601 tatgttacta gtacatcagc aaaagaggaa tttgatacaa atgatcaaac tttttataag
      661 ataactggta aacattcaaa agtaataaga gtaccttatg gaagcaaacc atatacacca
      721 caagcctctt atcaggcttt agttgatgct ggatataaaa tttgggactg ggacttagat
      781 acagaagact ggagatctaa ttcttctcaa attgtacaaa atgtaaaaaa tcatattaag
      841 aatagaaaag gtgaagataa agaccaactt gtagttttaa tgcatgagaa aaaacagagt
      901 gcagaagctt tagattcagt tttaaaattc ttatcagatg aaggttatga atttgcgcca
      961 gtagatcaaa accaaatacc aaaaaattat tggttacgta atttagagta gttaaaatat
     1021 aagaatattg attgatattt ttagaaattg agaataaaaa tgagtgaggt gtaggtatat
     1081 tgaataaaaa actttatata ggaatggtcg gaatattgtc tttgatgatg gttggttgta
     1141 ataaaagttt agcaaaagta aatgatgtgg aaatcacaaa agaacagtac aaaaaaacaa
     1201 aagcagtttt atctgctact aataattata taaatgggca gtctttagat gaattagaaa
     1261 aaactttgga taaaaaaggt agaaataaat tagaaaatgt tataatatcg tttatggtgg
     1321 ataatgaact tttatatcaa gaggctaagg ataaaggact aactccaagt aaaagtgaag
     1381 tagactcaaa atatcaagaa ctagaagata agatgaattt gaatacaagc tataaggaaa
     1441 aaatggacaa agcaggtgtt gataaagatt atttaaaaca agaaatatct agagatttag
     1501 caatagataa aaataaaaag gcctttgaag atagaataaa cataagtgat aatgatatgg
     1561 aagcctacta tacaagtcat aaaaaagatt ttaatgtaga agaagtaagt gcttctcaga
     1621 tacttatatc gactttagat aaaaataaaa aagaagtaag caaagataaa aaagaagctt
     1681 tgaagaagaa ggcagataat atattgacta aaatcaaaaa tggagaaagt tttgaaagtt
     1741 tggctaaaaa atattcggat gataaagcaa ctggaaaaaa tggaggacag ctaggatatt
     1801 ttacaaagga tgataaaaat gctgagttta caaaggaagt gttcaaatta aagaaaaatg
     1861 aagtttcaaa tgtgtttgaa actagttatg gatatcatat agtcaaagtt actgataaaa
     1921 gagaaagaca aaaaagtttt aatgagtgtc agagtttaat aagagaatct attttaaatg
     1981 aaaagtatat tgagcatata aaaaaactga atgaagatgc taaaatagac agatagaatt
     2041 tctaacagct caatcctagc ctgtaatcgg agggagattt tacaggaatg tttaagagtt
     2101 taaagagaac gaaagttgag aaatacatta tcgataataa agactctttt tatagaatag
     2161 catatagtta tacgaagaat gaagaagatg ccttagatgt ggttcaagaa gccatgtata
     2221 aggcactata ctcagtggaa aatattaaag aagtaaatta tataaagaca tggttttata
     2281 aaattttagt tagaacgagt atagatttta ttagaaaaaa ccgtaaatat aataatatga
     2341 cagatattga ccttatagat gagactggag agtatgataa atatactgat ttggatttaa
     2401 gaagagcttt ggaagaatta cctattgaat acaaatctat tataatatta agattttttg
     2461 aagatttaaa gattgaagaa gtagcaataa tattggatga aaatgtgaat actgttaaaa
     2521 ctaggttata tactgcttta aaaaaactaa aattaaaaat tgaagagtag ggataaaaag
     2581 aatgacaagc aaagaaaggt taaataagtt aaaagatgag tatcataaaa ttccaatacc
     2641 caagaaatta gatactataa taaatcatga aaaaatagaa aacatatatc gagagaaaaa
     2701 taaaaaagta aatagattaa gatttaaagt tgcaatagcc tttgcatgta tatttacagt
     2761 attagtaaat ataagtcctg tatttgcaga caatttttct aagatacctg taattggagc
     2821 aatagtagaa gtaataacta taaaaaatta tagtttaaaa agtgaaaatt atgaagctga
     2881 gattgatatt cctaaaatac gaggtttaaa agataaaaac ttagaacaaa gattaaacag
     2941 tagttttatg gaagatggaa aaaggcttta tcatcaattc caagagagaa tggagaagat
     3001 acaatcatca aagaataaag gatataaatc tttaagtttg agttatagtg taaaaaataa
     3061 ttctaaaaaa tttctatcaa tagagatgac aaaaaatgag atagaggctt catcatatgt
     3121 gagtaaggta cattatacta ttgataaaaa aagacaaata gttttgactt tgccaatgct
     3181 ttttaaagat gataagtata taaaagttat aagtgataat ataaaagaac aaatgagaga
     3241 acaaatgaaa aaggatagta caaaatccta ttttatagat caaaaaaaag acttgcctgt
     3301 tgaagatttt aaaacaatta ataagtatca ggatttttat tttaataaaa atgaagacct
     3361 tgtaatttgt tttgatgaat atgaagtagc acctggttat atgggcgcag ttgagtttgt
     3421 aataccatat aaagtaataa aagatttgta aattgaatag tagtttatat ataaatcttt
     3481 taaggaggac aatatgacta catataaaaa acttaaaaaa tctatattta aaattgctat
     3541 attttcaata tgcttattag cttcattagt agatacaaac cctatatttg caggtagttc
     3601 ctttaatgaa acttcaaata gtgatacgat aaaggtgtta aatgctaaga gtatcaactt
     3661 taaacaagac aattatgaag tgaatgtgag tattcctaaa ataaaaggtt taaaagataa
     3721 aactttagag aataaattaa acaatgagtt tttgcaagat gggaaaaaaa tttacagtga
     3781 atttcaaaat aaaatgaaaa aggaagataa taagggtcat agatacctaa atgtaatcca
     3841 taatgtcaaa aataattcta aggacttttt atcaatagaa atgacaaaag aagagataga
     3901 agcttcctca tctataacta agaagcatta cactgttgat aaaaatagag gaatactatt
     3961 aaatttacca atatttttta aagatagtag atatattaga gctataagtg ataatataaa
     4021 agagcagatg agaaatcaaa ttaagaaaga tgatacaaaa tcttatttta tagaccaaga
     4081 taaagactcg ccagtgaaag attttgaatc tataaataag tatcaagatt tttattttaa
     4141 taaaaatgga aatcttgtga ttagttttga tgaatatgaa gtagctcctg gttatatggg
     4201 agcagtagaa tttgtgatac caaataaagt aataaaaaga ctaagataga tatatagaag
     4261 atttttagat agactggtaa ggagaattat atgaatatat atgaacaagc aataagatta
     4321 atagaaaata atgaggattt cgcatttgct acaataacgt ctcattcagg ttcaacacca
     4381 cgtgaaactg gagcaatgat gattgtgaaa aatgattcta ctatatttgg ctcagtagga
     4441 ggaggtagtg tagaagcaga atgtataaaa catgcaatta atgttataaa aaacagagaa
     4501 tcaatgttgt ataaatttac tctaaacaaa tctgatgttg caaaactagg aatgatatgt
     4561 ggaggtactg gagaaataca aatagatttt atagatagta aattaaaaag taatatagaa
     4621 aaatttaata agagactaaa ggaaaataca agtaaagcat atatatttgg tgcaggtcat
     4681 atttcaagag atgttgctgt gatactatct cttttagaat ttagaacagt agtaatagat
     4741 gatagagaag aatttgctaa tcatgaaagg tttccagaat cagaagtagt agttttagat
     4801 tcttttgaaa acataccaga ttttccaact gacgaaaata gttatataat tattttaaca
     4861 agaggtcatc tatatgattc aagtgcttta gaatgggctc ttaaaagaga ggctggttat
     4921 ataggtatga ttggaagtag aacaaaaatt ggattgactt acgaaaaact catgaaaaaa
     4981 ggttttaaga aggaagaatt atcaaaagta catgcaccaa taggcataaa attgaatgct
     5041 caaacaccag ctgaaatagc tgtatgtata gctgctgaac ttataaattg tagagctaat
     5101 aaggaaaaat aaactaatga acacagggag taaagatatt gatactttaa aggttaattt
     5161 tagagggatt gaaataaaag cagttttagt atataaaaat agaaaaaata tttctataaa
     5221 aatagatcca tttggcaaaa taactgtaat gagtcctcct aaaatatcca aaaaaataat
     5281 aaaagatata attattgaaa aaggagactg gatacttaaa aagtcagaaa aatgtaaagg
     5341 aagagaggaa gtatataaac aaagaatgtt tataactggc gaaaaatttc tttatttagg
     5401 tgaagagtac catttagtta taaaagaact gttgagagat tctgtcaaaa aaagtaactg
     5461 tagaataact attaacgaat atcaaatagt tgttcagaca aatgatacaa gtacagattt
     5521 tattaaaaaa tctttaaaat cttggtataa gatggaaagt gaaagaatag tattagagag
     5581 aatagatttt cttaaattaa attgtgaaat aatgaagcaa ttagttccag cgagtgttaa
     5641 ggtaaaagaa caaaataaga gatggggaag ttgtacagct caaaaaaata tttatataaa
     5701 ctcaaaaata tctatggcaa gattagatgt aattgattat gtaataatac atgagtttag
     5761 ccatttagta catatgaatc actctaaaaa attctatgat ttagtaaaat ccattatgcc
     5821 cgattataaa gataaagaaa attggctaaa aataaatgga tataaaataa taatctgaaa
     5881 acgattattc caaaatatag gcgtaaagta taaaagaagt attattgtga aaaaaataaa
     5941 taatctgaaa attttaaaaa aagtcattga cattgtaaat gatagggaat ataatctata
     6001 ttaacaacaa aacagaatta aaatataaaa ttaaaaaatt aaaagcaatg acagagacaa
     6061 caatactaat agtgttatcc acagagaacc aatgttgctg agaattggtg ttaacagtag
     6121 tataatgatc actctggagc ttttgtactg aatattttta gtaagtacaa acgtatgtca
     6181 gcgttacatg actaaggtta aggtaattaa ttacccgacc tgaaaaaaga ggggtagatg
     6241 tattttttat atctacaaat aaggtggtac cgcggaatat aactttcgtc cttataagta
     6301 atatttatat tgcttataaa gatgaaagtt tttttatttt aagcgatata aataaattta
     6361 atccatataa aattaataaa aaaataataa aaataaggag ggatttttat ggaaaatact
     6421 gtatatgcta aattaaacca aggaatagat tcttttttaa gagtggctat gactctaaga
     6481 agaagagagg ttgatataca atcaataagt atgactgtag ataatatgaa taattcaggt
     6541 ataaaattaa tagtaaatga agaaaagaca tccttagatt ctgtcttaaa ccatatgaaa
     6601 aaactacatg atataagaga gataactgtt gaaagaatga gacattaatt aaatatattt
     6661 tataaagcta aagataaata tgcttatttt aaaataaaaa actaaagata aatattaaat
     6721 aagaaaaatc ttagattaaa atataagtgt cattgaattt attagtttat cacagttaat
     6781 aaatttatta gtttgttgta gctaataaaa actgaaaatt ctaaaattat aattgtaaga
     6841 ttatataaat ttagaattta aatctgttga ataatattaa aacaatgata atgagtatga
     6901 atttctaata aaaataaata tcataaaaat aagaaaaagt attgggagga aatgaaaatg
     6961 gcaagaatgt attatgaaaa agatgtggat ttagaagttt taaaaaataa aaaggttgca
     7021 gttttaggtt atggaagtca aggacatgca catgcacaaa atcttagaga taacggtgta
     7081 catgtagtga taggtcttta tgacggaagt aaatcagcac aaaaagcaaa agaagatgga
     7141 tttgaagtta agagtgtagc agaagctaca aaagaaagtg atttgacaat gatgttaatg
     7201 cctgatgaaa aacaaaagaa agtttatgaa gagagtgtaa aggataactt aaaagaaggc
     7261 cagacattag cttttgctca tggattcaat atacattata atcaagtaca accaccagaa
     7321 tttgttgatg ttgtaatggt agctcctaaa ggacctggtc acttagttag aaatgtattt
     7381 acaaagggaa gtggagttcc tgcactattt gcagtatatc aagactacac taaaaaagct
     7441 acagagacag ttttagcata tgcaaaaggg ataggagcaa caagagcagg tgttttagaa
     7501 actactttta aagaagaaac agaaacagat ttatttggag aacaatcagt actttgtggt
     7561 ggaataagtg aacttataaa attgggatat aaaacgcttg tagatgcagg ttatcaaaaa
     7621 gaagtagcat attttgaatg tttacatgaa atgaaactaa tagtcgacct tatatatgaa
     7681 ggtggatttg aaagaatgag atatagtata agtgacacag cagaatatgg agattatgta
     7741 tctggtaaaa gggtaataac tgatgttgca aaacaaggta tgcaaaatgt tttagaggat
     7801 atacaaaatg gaaaatttgc aaaagcgtgg attaaagaaa atgaagaagg acgtgaaaac
     7861 ttcctaaaaa ctagagaaga agaatataat acagaaattg ccgaagttgg tagaaatcta
     7921 agaagtatga tgtcattctt aaaataaata tatattaaat ggattagttt gagggaaaga
     7981 gtggtgtata gtagtgcgta tgaatggagc aaaggtaatt ttagaatgtt taaagaaaga
     8041 gggaattgac acaatatttg gatatcctgg tggagccgtg atacctcttt atgatgctct
     8101 atacgattat tcagatgatt ttaaacatat aaggacatcc catgaacaag gtttagttca
     8161 tgctgcagat gggtacgcaa gaagtactaa tacagtagga gtttgtttta caacatctgg
     8221 tccaggagca acaaatgcca taactggaat agcaacagct tttatggatt cttcacctat
     8281 ggttgtaatt tcagggcagg tgccaactag tttattagga aaagattctt ttcaagaaat
     8341 tgacataact ggagcaacat tatctatgac aaaacataat tatttagtta gaaacactaa
     8401 agaattagtt cctacaataa aagaagcttt tagagttgca aattcaggta gaaaaggacc
     8461 tgtacttgta gatgttccaa aagatttatt tttagcagaa atggatttta gtggtgagga
     8521 ctatgattta tgtcaaatag atgattatat ggattataag agtgattttg atctagatga
     8581 tgaaactaat attaagcttt taaatgaagc aatagatatc ataaaagaat ctaaaaagcc
     8641 tataatatat gcaggtggag gtgttaaatc ttcagacagt gaggaaattc ttgaaaaatt
     8701 tgcaactaag atagatacac ccgtactaaa tacacttatg gggcttggaa atatagatag
     8761 aaagaatgaa ttatctcttg gaatggttgg aatgcatgga agtagagaaa gtaatttagc
     8821 actttctaat tcagatttag taatagcgat aggtgcgaga tttagtgata gagtgataag
     8881 taaaagttct gaatttgcta aaaatgcaaa aataatacac atagacatag acccatctga
     8941 aataagtaaa aatatagaat ctaatgtatc tttggttgga gatgtaaagc tagtccttag
     9001 tttattaatt gaaagggttg aaagtaagaa taatagcaat tggaaagaag agataaagag
     9061 atttagaaag agtgagggtg tacaaacata tgaatttcat ccacaaaata tacttaaaaa
     9121 aataaatgaa aaatatgaaa cattaaagaa acccactgtg gtagtaactg atgtcggtca
     9181 acatcaaatg tgggctgcta agtactggaa ttttaaaggt aataaaagtt ttataacatc
     9241 agcagggtta ggaactatgg gatttggttt aggtgcagct attggaacta aagttggtaa
     9301 tgtagataaa aatgtggttt tagtcactgg tgatggaagt tttagaatga actgtaatga
     9361 actagctaca gttgcaaatt ataatgtacc tatgcttatt ttactactca ataatagaac
     9421 attaggaatg gtaaggcaat ggcaaaagtt attttccaat caaagatact ctcaaactga
     9481 cattaatgaa aatgtggatt atgttaaact cgtaaatgca tataacattg atggatataa
     9541 ggtatccagt atggaagaat taggaaaggc gctagatatg atagatttta ataaacctgt
     9601 atttttgcaa tgtgatatag ataaggatta tgatgtttat cctattgtag cacctaatga
     9661 tgcattggaa aatttgatat gtaactaaaa cttatactga atattgataa aattttttat
     9721 taaattttta ttagtattca gtttttgttg tttagagata gattggcatt ttttattttt
     9781 gcaaataaat caatgactat ataattaaga acttattaaa ttttaattat aca
//
LOCUS       DAMJYX010000115         9719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJYX010000115 DAMJYX010000000
VERSION     DAMJYX010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.120"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..884
                     /locus_tag="PC479_003021"
     CDS             <1..884
                     /locus_tag="PC479_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2805545.1"
                     /translation="TISNNIILYTWIITVVVYLIYTIFKYIKLKNLINDLSYDVDDGE
                     IVNLYKNILEEFNITKDIKLKYSYEVETPAFFNSCVLLPPHEYKLKELDWIFRHELMH
                     FKSRDLYLKYVILFLKTVYWFNPFIYIMDKHIDLDCELYCDERVLKNRNSEEKKDYAL
                     TILNAMRKGSNTSNKFIAGLHKQSDIKKRVMNMFNEKYKNGILMALTLCLLSSITFLK
                     VNSTSIVNLNILPKGMEEKDVTNSAIVTMIEFTYAEAPKSMREEHKINCEFLGLIPKD
                     SDKIGVGIEYYDSLLND"
     gene            1800..2822
                     /locus_tag="PC479_003022"
     CDS             1800..2822
                     /locus_tag="PC479_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2805546.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3118..4038
                     /gene="pyrB"
                     /locus_tag="PC479_003023"
     CDS             3118..4038
                     /gene="pyrB"
                     /locus_tag="PC479_003023"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2805547.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4077..4769
                     /locus_tag="PC479_003024"
     CDS             4077..4769
                     /locus_tag="PC479_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2805548.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            4762..5664
                     /locus_tag="PC479_003025"
     CDS             4762..5664
                     /locus_tag="PC479_003025"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2805549.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            5749..6333
                     /locus_tag="PC479_003026"
     CDS             5749..6333
                     /locus_tag="PC479_003026"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2805550.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6547..7473
                     /locus_tag="PC479_003027"
     CDS             6547..7473
                     /locus_tag="PC479_003027"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2805551.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(7525..8415)
                     /locus_tag="PC479_003028"
     CDS             complement(7525..8415)
                     /locus_tag="PC479_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2805552.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8603..9634
                     /locus_tag="PC479_003029"
     CDS             8603..9634
                     /locus_tag="PC479_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2805553.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
BASE COUNT         3848 a         1008 c         1701 g         3162 t
ORIGIN      
        1 caactataag taataatatt atattgtaca cttggattat aactgtagta gtttatttaa
       61 tttacactat atttaaatat ataaaactta aaaatttaat aaatgactta tcttatgatg
      121 ttgatgatgg agagattgta aatctttata aaaatatatt agaagaattt aatataacaa
      181 aagatataaa actcaaatac tcttatgaag ttgaaacacc agcatttttt aattcatgtg
      241 tgcttttgcc accacatgag tataaattga aagagttaga ctggattttt agacatgagc
      301 ttatgcattt taagagtagg gacctttatt taaaatatgt aatcctgttt ttaaaaacag
      361 tatattggtt taatccattt atatatataa tggataaaca tatagattta gattgtgaac
      421 tttattgtga tgaaagagtt ttaaaaaatc gaaattcaga ggaaaaaaag gattatgctt
      481 taacaattct aaatgcaatg agaaaaggtt caaatacttc aaataaattt atagctggac
      541 tccataagca atctgatatt aaaaagagag ttatgaatat gtttaatgaa aagtacaaga
      601 atggtatatt aatggcatta actttatgtt tattatcttc tattactttt ttgaaggtta
      661 attcaacgag tattgttaat cttaatatat tgccaaaagg aatggaagaa aaagatgtta
      721 ctaatagtgc aattgttaca atgatagaat ttacatatgc agaagctcct aaaagtatga
      781 gagaagaaca taaaataaat tgtgaattct taggtttaat acctaaagac tctgataaaa
      841 taggtgttgg tatagaatat tatgacagtc tattgaatga ttaaataaat attattttaa
      901 cagatattaa ttataaagtt ttgaatagta atttgttgag aaataaatat atccttataa
      961 aaatagatac ttatatacca agaataaaaa atagtcaaga tagtacacaa ggatatactt
     1021 ataaagaact caaataagtg aaataaatta ttaattataa ggtttataat tttaaaaaat
     1081 tagataaatt caggtgtaaa atactataaa acacaattcg gaaaagtgta tgttgaactt
     1141 catttaaaat ggaatttcaa agttgctata gtaagtagtt taagcattct tcttatatta
     1201 atctttgaga ataatgtatt taaaaaagtt agtaaaaaat ttaactattg tatattaatg
     1261 atagttgcta ttgatatatt atctaaaaaa attaagtatg aaaagattac tgctaggaca
     1321 atcttttcat acttaatttt taatatttat attatccaaa attttcatct ttttaactta
     1381 gaatttgaga atcactatta ggaaataggc tttgatttat gtagggttaa aaatttcaca
     1441 gaatatctaa ctttatacta aatccatcaa aaaatatacg ccatataaaa gcgcataaaa
     1501 aaacacataa aacaatattt atgcgcactt taacctcgaa tggtacaaaa ttctttactt
     1561 ttacagaccc atattatata attaagtaaa taatgtatat ttaggtgaca tattggataa
     1621 gctaatttat ttattttatt aatataatga tactataaaa gttatataaa gtggtttaca
     1681 aaaaagttac agatagagct tttattaatg tattgtgtat tagtatttta tccttaggta
     1741 cacttcacct acaaaataga atcataagat aattatggga aaattttagg aggtttatcg
     1801 tgaagaaaaa gatattaata ccagtatttg catcagttat ggcattatca gtaagttcaa
     1861 ttgtaaatgc agatgaagta aatgattcat ctcaaaataa ggatgataaa acaaatactg
     1921 aattaaattt aggtgaatat aaagaagtaa aatataaagt tgcaaaaata aaagatggag
     1981 tagctataaa gataagagaa gaaggtcaag ttcaaaacat agcttattct ggagatgaat
     2041 ttacggtttt aggaactcaa ggagaatggg ttaaggttaa agttgaagat ggcgaaggtt
     2101 ggttagctac tagatatgta gatatatctg aaggtgtagg atatactaac gctgataaag
     2161 taaacttaag aaaagataag agtgaaagtt cagaagtaat tgaagagctg gaaaaaggaa
     2221 gttctttatt ggttttggaa gataatggag attggttaaa agttaaagat ggggaaacag
     2281 aaggatatgt aaaatcatct tatatatctg ataaagctcc tgtaatagaa gagccacaag
     2341 taaacccaag tattgaccaa aatgtagatt ctacaaataa taatcaacaa aacaatgcaa
     2401 atactaatca acaaaataat aattcaaatg tgccaacagc taactctaat gcagttcaag
     2461 ctgtacttaa cttggcatat tctaagcaag gttgtccata tgtttgggga gcagaaggac
     2521 caaacacttt tgactgttct ggatttacac aatatgtgta tagaaatgca gtaggaaaga
     2581 atataccaag aacatctaaa gcacaatcta aatatggaca aactgtaagt aaagcaaatt
     2641 tacaaccagg agacttagta ttcttcacaa ctaatggttc tggttctgtt agtcatgttg
     2701 gaatatatgt aggtgggggt aatatgatac attcaccatc aacaggaaaa acagttagtg
     2761 taactagtat aaattcatca tactatacag caagatttgt tacagcaaaa agaatattat
     2821 aatcggtttg taatatgaca aattgataaa tacataaata tatataaacg ctctatatta
     2881 tatttaattt aatcatggaa acaaaaaatg attatatttt aaatgtacta tagagcgttt
     2941 ttgtatcact ttaaatctaa tcaagattca agttaatatg actgttaaat aggagccttt
     3001 tcatatgatg attatttgcg caatcccttg acaggatagg gcaaaggtgg taagctacta
     3061 acagtacaaa aatactttta attttagttc agagaggcta aaaaaggagg acgaaagatg
     3121 ttaaaatcaa gaaatttaat ccagccagaa gacttttcaa tagaagaaat agatgaaata
     3181 ttagaattag ctcaaaaaat aattgacaat ccgtcaaaat attctcgtat atgtgaagga
     3241 aaactattag ctacattatt ttatgaacca tcaacaagaa caagattaag ctttgaatct
     3301 gctatgaaca gattaggagg acgtgtagta ggcttttcag agccaaattc atcctctgca
     3361 tcaaaaggtg aaactctagg agatacaatg aggatagtat ctggttatgt tgatataata
     3421 gctatgagac atcctcaatc aggtgcagct tcagaagcag ctagatatac ggaagtgcca
     3481 tttataaatg caggagacgg caaaaaccag catccaactc aaacacttac agacttactt
     3541 actataaaat cattaaaagg aactttagaa agccatacaa taggattatg tggtgattta
     3601 aaatatggaa gaactgtgca ttctttggtt aaggctatgg caagatataa aaatacaaag
     3661 tttgtattta tagcgcctga agagttaaaa atgccagatt atataaaaga agctataaag
     3721 ggacatgcat attatgaaac taataatttg gatgatgtta taggaagctt ggatgtttta
     3781 tatatgacta gagttcaaca agaaagattt gaagataaat cagagtatga aagacttaaa
     3841 aactattata ttttaaataa agctaaatta gaaaaggcat ctaaagatat gttagtaatg
     3901 catccattac caagagtaaa tgaaatagac atagatgtag atagtgatga tagagctgta
     3961 tattttaaac aagctaaata tggaatgtat gttagaatgg ctttaataat aaaactttta
     4021 ggtataaatg aagactaata gttatcatat aatagtaaaa caaaggagat tttgggatgt
     4081 ataaaattct ggaaaatata tatataggcg aagatatgta tagaatgaaa gtaaaaggta
     4141 attttgaagg taaaatggga caattctata tgttaagagc atgggatact tatccagtac
     4201 tttcaagacc tataagtata catgatatag atgaagaagg gataactttt ctttataaag
     4261 tagtaggaga aggaactcaa attttgagta acctaaaggt aaatgataat ataaaattag
     4321 aaggccctta tggtaatgga tatgctaagg ttgatggaaa ggtagctcta gtaggaggag
     4381 gaatcggtgt agctccacta tatttggttg ctaaaaatat aaaaaattgt gatgcatatc
     4441 ttgggtttag agaagatgtt atattagaag atgaatataa acaagtatgt aataaagtct
     4501 atacaacagt aggaaatact tttgtaacgg atataattga tgttgagaaa tatgattata
     4561 tattaacatg tggtccaact ccaatgatgg aaaaattagt taagatggta gaaggtacta
     4621 aaacgagaat tatggtatct cttgaaaacc atatggcttg tggagtaggt gcctgtttag
     4681 tatgtacgtg taaaactaat ggaggaaata aaaagacttg taaagatggt ccagtgttct
     4741 ggggagagga cgtgattttt aatgggtaat ttaagtgtga aatttggaag tgtagaattt
     4801 aaaaatccag taataatggc gtctggaact tttggatttg gaaaagaata taatgagatt
     4861 tatgatatac aaaaacttgg tggtataagt agcaagggtc ttactttaaa taaaaaacct
     4921 ggtaacaatg gtatgagagt tcatgagaca tcatccggta tgatgaatag tgttgggctt
     4981 gaaaaccctg gagttcaagg atttatagac tatgaattac ctttttttag taaactagac
     5041 ttagtcagaa tagctaatgt tggaggtggg acattagaag actacttatt aggggttcaa
     5101 atgttaaatg acaagcctat agacattatt gaattaaata tatcttgccc aaatgtcaaa
     5161 gctggtggaa tggcttttgg tataaaaaat gaagtggcaa gagaagttgt tcgtgaggta
     5221 agaaacataa cgaaactgcc tttagtaata aaattatcac ccaatgcaga agatattgta
     5281 ggaatggcaa aagtttgtga agaagaaggt gctgatggag tttcattagt taatactttt
     5341 aaagcaatgg caattgatat aaaaaataga agaccagtat ttgaaaatgt atatgctggg
     5401 ttatctggac cagctataaa acctatagct ttgagaatgg tacatgaagt gtgtaaaaat
     5461 gtaaatatac ctgtaatggg aatgggcgga ataactaaag ccacagatgc tatagagttt
     5521 ataatggcag gagctacttg tatccaagtt ggaactgcaa attttatgaa tcctagaata
     5581 ggtattgaaa taatagatgg aattaatgaa tttatggata gagaaggaat aaaaagttta
     5641 gacgaaataa gaggaattat ataaggtaga ataaatagta ttttaaagat aaaaaatata
     5701 gattaaataa acaataattt tataacatta acatggagga aaaataatat gagtaaagta
     5761 gatgtagtgg atatattaaa gaaaagtgat gcattgttgg agggacattt tttattatct
     5821 tcaggaaaac acagtaatag atacgtacaa tgtgcaaaag tattaagatt tccagaatat
     5881 gctgctgagg tattaagtac agttgttgaa caaataaaag acttagatat agacttagta
     5941 gtaggaccag ctatgggtgg agtaatagtt tcttatgagt taggaagaca attaggaaaa
     6001 gaagctgtat ttactgagag aaaagacaat acaatggagt taagaagagg atttgaagtt
     6061 aaaaaaggag caaagataat aattgctgaa gacgttgtaa ctactggtaa atcaactatg
     6121 gagacaaaaa gagtattaga agccttaggt ggagaagttg taggtgttgc atgtatagca
     6181 gatagaacta atcatgatat aggtatgcct atatatagtg ctataaaact tgatattcaa
     6241 gtttatgaat ctgatgagtg tcctttatgt aaggaaggaa aattaccagt tgttaagcct
     6301 ggaagtagag agttcaagga attagggatg taataaaaac ttaattattt atagtgttac
     6361 ttaaaaaatg taaatttttt agagtaaaaa tttgtaatat aacatataac ttaaaaataa
     6421 aaagcagtta gccttataaa tgaaggttag ctgttttttt gttaaaaata tatttacaaa
     6481 ataagagata gtagcagtac aatataagta taaggtaaaa gtattatata ttagggaggg
     6541 gtaagtatga aaaatcaatt aaatgaaaat cataaaaagg ttgtattttt tggagtaggt
     6601 gctgtaggag ctacttttgc agaacaattt tttaactcta aatatgattt taaaattctt
     6661 tgtgataatg aaagaaagaa aagatattta gaagaaggat ttataataaa tggaaaaagg
     6721 tatgattttg attatgtaac taaagatgag tataaacaag aggctgattt tataattata
     6781 ggtctaaaat ataataattt aaaagaaaat ataaaagaat tagatggatt agttggaaaa
     6841 aatacagtta taatgtctct gctaaatgga gttgatagcg aagagataat aggagaaaga
     6901 tttggaattg aaaaaatggt atattcatat gttaccaata tagatgcaaa gaaaatcaat
     6961 aataatatta tacacactac taatgggata attgtatttg gtaacaaaga taatagtgaa
     7021 gatagaaaaa ctaatataat aaccgaagtc tttgatgatg taaatataga atatacttta
     7081 tcaaaagata ttcaacgaga tatgtggtgg aagtacatgg ttaatattgg tgtaaatcaa
     7141 acttcagcta tacttggtgc accttatgga gtttttcaga gttctgagca tttaagagaa
     7201 ttagcaaaat ctgcaatgag ggaagttgtt gctatagcac aagcaaaaga catatctctt
     7261 acagaagatg atgtggaaca ttcattacat agaatactag aacattcaaa agaaggaaga
     7321 acatcaatgc ttcaagatgt ggaagctcat agacttacag aagtagatat gttttctaag
     7381 aatatctgca aacttggaaa aaaatataac atacctactc ctataaatca gactttcttt
     7441 tatatgataa aagtaattga aagtagattt taaagggtct ttgtaagaaa tattgataat
     7501 tattgatttt aaattttatt aatgttatat actatttaag ttactaaata tttttaactc
     7561 ttcaatgaaa atagttgctg cttttgataa agttatatct tttggaaata tatatgagaa
     7621 atgtctgttg tagctgcttc caagctctat gacagacagc tcattgttta gaacaggtaa
     7681 gcttgtcact agattagaga ctattgtaat tcctaaatta tttctaacag attctttgac
     7741 agcataatta cttccaaaaa ccatcatact ttttggaata atttctttaa cacttaaaaa
     7801 catatctaaa taatctctgg ttccagaacc atcctccctg actatccatt tttggttttg
     7861 aagtttatca aaactaaaat cttttaataa atgactcttg tatggaagtg ctaaaaccat
     7921 cttatcttca aaaaaatatt cttgaataaa tgaaggggaa gagcaagtgc cttcaataag
     7981 accaatgtct aaaatataat ccttaacatg agaagatacg attgatgtat tttttataaa
     8041 aacttctaca tctatgtcag gatacttttt agaaaataat gctagaaaat ttggtaaaat
     8101 atactctcca atagttaaac ttgcaccaat ctttaagtga ccagttatag catctgaaac
     8161 ttggcttaca tcatgataag taatatttaa aagatttaat atttctttag ctcttttgta
     8221 caagatttgt ccactttctg ttataaaaat ggttttttgt ttaatagacc tgtttataag
     8281 cacaacttta aaataatttt ctagattttt tatgtgttta cttacacttg gttgtgatag
     8341 atttaaatat tccccagctt tagtaaagtt tttgtattca acaacagcta caaatgtttt
     8401 taattcttca aacaattttt tgtctccttt acctaatgaa attttacatt ttttatatac
     8461 tttctacaga taattatact atatataata accattaaaa acattaatca ttacgatatt
     8521 atatatttat tttacttatg gttattacaa ttatatacta ttgctatgaa gaaaactata
     8581 ttaattataa ggtaggtata ttatgttaaa gaaaaaaagc agtttgacat taaaaactat
     8641 aagggaaata ttaccaggac tgtttgtatc agtattagta ggttatatta gtatatttat
     8701 ttctatttta attcctaagg taggggcagc atcaatttct atatttttag gtatgtttgt
     8761 aggaaaccta tttttaaatc aaaaggtatt tcaaaaaggt tataaatttt cagagacaga
     8821 tttgttatca tattcaatag tactattagg agcaacactc agtgtatcta ccttaataga
     8881 cttaaaagtt tcaggaattt tatttataat attgcaaatg acaataacaa taatagctgc
     8941 actttatata ggtaaaaaac taggatttga agaaaacttt agatttctaa tggcaagtgg
     9001 aaatgcagta tgtggttctt ctgcaatagg agcaacagtt ccagtggtta atgcgacaga
     9061 taaagaaaag ggaattgcag taactattgt aaatgttact ggtatatttc ttatgttttt
     9121 acttccaata atatctcaat ttttatatag ccatgaactt gtaaaaactt cagcaatgat
     9181 aggtggaact ttgcaatcaa ttggtcaggt tgtagcaagt ggagcaattg ttggagaaaa
     9241 tgtaaaagat ttggctacta tttttaagat agtaagagta atatttctag ttgttgttgt
     9301 attagtattt ggacatatga aaaatcaatc taatagggaa attcttgaag aagaaaagga
     9361 tgagataaaa aagaaaaaag tgactatacc atggtatgta attggattct ttataacttg
     9421 tgcacttttt tcaatgaaca ttataccaaa agaagtatct gtgttgtgca aagaaataag
     9481 taacaaacta gaaataatag ctttagcagc aattggatta aaggttaatg taaaagattt
     9541 agtgaaacaa gggaaagaag tttctttata tggtttattt gtaggtatag tacaagttgt
     9601 atcagctgta gttttaataa aaatatttat atagcataaa aatagtattt gtaatattaa
     9661 tttgattaac taaaataata tgatactaat aatttataat ataaataaaa aatgaattt
//
LOCUS       DAMJYX010000116         9663 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJYX010000116 DAMJYX010000000
VERSION     DAMJYX010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.121"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..280)
                     /locus_tag="PC479_003030"
     CDS             complement(<1..280)
                     /locus_tag="PC479_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805554.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTA"
     gene            complement(563..859)
                     /locus_tag="PC479_003031"
     CDS             complement(563..859)
                     /locus_tag="PC479_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805555.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(878..1291)
                     /locus_tag="PC479_003032"
     CDS             complement(878..1291)
                     /locus_tag="PC479_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805556.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(1275..1508)
                     /locus_tag="PC479_003033"
     CDS             complement(1275..1508)
                     /locus_tag="PC479_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805557.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1727..2101
                     /locus_tag="PC479_003034"
     CDS             1727..2101
                     /locus_tag="PC479_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2805558.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
     gene            2713..3405
                     /locus_tag="PC479_003035"
     CDS             2713..3405
                     /locus_tag="PC479_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805559.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3601..5661
                     /locus_tag="PC479_003036"
     CDS             3601..5661
                     /locus_tag="PC479_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2805560.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(5866..6189)
                     /locus_tag="PC479_003037"
     CDS             complement(5866..6189)
                     /locus_tag="PC479_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2805561.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(6182..6883)
                     /locus_tag="PC479_003038"
     CDS             complement(6182..6883)
                     /locus_tag="PC479_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2805562.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(7161..8546)
                     /locus_tag="PC479_003039"
     CDS             complement(7161..8546)
                     /locus_tag="PC479_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2805563.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(8654..>9663)
                     /locus_tag="PC479_003040"
     CDS             complement(8654..>9663)
                     /locus_tag="PC479_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2805564.1"
                     /translation="YDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIENFKKEIL
                     EIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNKELRFIF
                     KHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFHELCELSCDEIVIDKCTTSE
                     IKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRLDSMFNQDKKKINLGLKFLL
                     TFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPELIQTIG
                     RGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRFEI
                     N"
BASE COUNT         3713 a         1147 c         1332 g         3471 t
ORIGIN      
        1 atgctgttgt tataatggct actaatatta ttgctattat tgtaatatct aatcttcttg
       61 taaataagcc tattgctata tactcaagac ctgttacata gcgataaagt acttctagtt
      121 taacagtttt tttcttatct atatttccat atattgattc taatttatta aaatattttt
      181 tactacaaaa agcagtttta ggtttaatta aatatattat tgtagttcct attataacta
      241 ttattccaat aagtataaga ttgatatttc caaaatccat agcagatacc tcctattaaa
      301 tttgcttttc ttaatcaaag tatatcagta acacaagtac aaagagtatc taaaatatta
      361 caaatattaa caggatagat aaaactatag taaaaattgt aataataaag cgaacgaaaa
      421 ttatcatact aaaacataag acatgttata attgtattag atagaagatt aacatacacc
      481 aaagcactcc ttataataaa gagtgcttat ttttttgaaa ttcatcaata tataaactat
      541 caagaacctt attcaacaca ccttaaaatc gatttaaata gttctttttg ataacataat
      601 ttatatgata taataaaaaa atagaagtgt accaccaata cacttctata gctataacta
      661 agcattctct tgctggggga gtgctttttt catttctcca aacaacatta tttacttcat
      721 cagtaattat caaatcaatc tcattatctt tcaaaatctc tgttgcagta caaattagat
      781 tatctaagtc aaaacttcga gttatctcta gttgtttgtt atttacttct ctttttagta
      841 tataagtttt aaaatacatt tatatcaccc cttgtattta ctcttttttc tctaaaaata
      901 tattgataag taaatatgca acaaataaaa aaatctcaat gacacatagt gttatagcta
      961 taactagaag tgattgattt ttccatgcac tataagctat gatgaaaaga aatgtaaaag
     1021 taaataatgt ataattcata attttaacta agatatgact tgttttcatt gttaagtaca
     1081 agtctctttc atctgtacta tcgtgtaatt cttcaacaat gccttttctt gaaaaagcat
     1141 tacaaaaatt aaagattgct attacaatta aaagaaatga tgaaattagg aatcttttat
     1201 cttttaaaat gcaataaata actaaactag cacaacataa cacacaaact ataataccca
     1261 ctataaaact tcgtttactc tttactttca tgttttttat cctccatttc tttattttct
     1321 tttaaacaac atagttcttc aatcgttgta ccaaacactt gtgccatacg ataggcaagc
     1381 ataagagaag ggctatattg ctctttttca atagaaataa ttgttctgtt tgatacatgg
     1441 actaaatcag cgagttgttg ctgtgttaga cccatatttg ttcgaaattc tcgtactttt
     1501 gttttcattc cataacctct ttctatatga agctaacttc atgtgaagtt aacttcataa
     1561 tatcctaaaa tgagtaaaat gtcaatctca ttagataagt attataaaac ctaaaagaat
     1621 aatatggtaa aaattggatg gaattattta ccttaattct cttgtttgta aaactttatt
     1681 gatatagaat ttaacatata cataaatcgg caaaggggcg cgaattatga aaattagtaa
     1741 attaccagaa gcggaattaa aagttatgag atatatttgt gaagctaaca aggtattaac
     1801 ttctagggaa ttagttgctg caatggaaga aaaatatgag tggaatgaat caactacatt
     1861 tacagttttg aaaagattag agcgaagaga gtttttaagt acagagaaaa taggcaaact
     1921 tacacattat aatatactag taaaagagaa aaaatatcta aggtttgaaa caaaggaatt
     1981 tttgaagaac atacataaaa attcaatttc aagtttaatg tcagcgttac atggtgaaga
     2041 cgacgaagtg gatgaagata aattaagtga actcgaagaa tgttttaaga atttgaaata
     2101 agaaaatgac aaattgatac ataataaata aaatgaaggt atgaaattat aactttttct
     2161 accttcattt tgtttattat tctgaacatg ttaagaaaat gaaataagtt tgtattttag
     2221 ttatataaat tttaatagaa tatgtacagt atatagtaga tagaatgtat aaaaatagaa
     2281 tttaaataag tagctattat tgcagtgaaa tgaatgtttc taagtatatt tgggtggaag
     2341 aatggtggaa gaaaattagt gaaatgtaat tcttccacca ttcttccacc tagaaaatag
     2401 aaactgttta ataagaatta atatacatta attcagctga atgcaatcaa aatacttaaa
     2461 tgaaagtaat ctgaaaacaa cataattcaa atcaatttat tcaaataaac ctcaatttat
     2521 cttaaatctt aagaaatatt taagaattag gataatattt tcttaagatt tatattttat
     2581 tatatagtta taagggaaag ttacaaaaat gtaagccaaa gtaatataac aatttagtca
     2641 ttgttgagaa agggagtgaa agatattgta taatatagat aagtattatt atacaataga
     2701 ggaagtgaaa atatgtacaa tatattagta gtagacgatg ataaagaaat tgttgattcg
     2761 atagaaatat atcttaaatc agaaggattt aaaatatata aagcatatga tggaatgaaa
     2821 gctttggaaa taataacaga gaatgacatt catctaatac taatggatat aatgatgcct
     2881 aaattagatg gaatcaaagc aactgtaaaa atacgagaag aaagaaatat tccaataatt
     2941 ttaatttcag caaaaagtga agatacagat aaaataatag gattaaatat tggagctgat
     3001 gattatataa caaagccatt taatctatta gagcttatag ctagggttaa atcaaactta
     3061 agacgatatg taactttagg taattatgaa gatacatgta aagaaattct aaaaagtgga
     3121 gctttggagt taaatacact aacaaaagaa gttaaaattg atggtgaaaa cataaaaatg
     3181 actccaattg agtataaaat aattgaactt cttttagaaa ataaaggacg agttttctca
     3241 atagatgaga tatacgaaaa agtatggaat gaggaaagtt ttaattcaga aaatacagta
     3301 gcagtacata tcagacgaat aagagaaaag attgaaataa atccaaaaga accaagattt
     3361 ttaaaggtgg tgtggggagt tggttataag attgaaaaat tttagtggaa aaattataaa
     3421 agaaaagagt ttaatattta attgcatttt gattttagtc atttttacat ttacacaaat
     3481 gttaggaatt ttaatatgtt ttaatttttt ctctgagagt ttaatttact ctatggggat
     3541 tatgcttttc ttaaacttta ttttatataa aatactgaca atgtagatga ggtgggcaac
     3601 ttggagaaat atagagtatt aaagttagtt tcattaatca taatatctat ctcatttcca
     3661 ttaactatat tgttggtgac aataggactt gctgagccta aatacaatat acaaaaaact
     3721 tctacaaaga gtgggatttt tgatatatct gatgattttt atgatagtaa tatatttgac
     3781 gaaggaacaa tggcaagtct attacccaag tttaagctat ctgtaggtgg taaattaact
     3841 agtaaagaaa aaggtaaatt ccaacaagaa ggtaaaagta tagatttaag tagtagtgat
     3901 tctgagtatg ataaaatcat gattaatgaa caagcttctg aagaatattt aagttatcta
     3961 aaaaatataa aagtgtatgt tattaataca tacacaggag tattttatac aaatacgtca
     4021 tatgctagtt tatatgattt taaacaaaaa actaagagct attgtaatgt agagataata
     4081 aataagatag gaaagagctc atatattaaa acaataaatg gagaaaaatt tgaactgaat
     4141 aatctgagtc tggaactaaa tgaagtagat gaaggttttg aggcatatgt atcttttcca
     4201 gaagagccaa ctatagaaga tggaatagta tacactaatt ttcaaatatt taaacaagct
     4261 acagaaaaag taagattatt ttttgtagct agtttaagtt tagcaattat atgtttatta
     4321 tctataattt tatatatcaa aataaaaggt gaagttttaa aaagaaataa tgtattttta
     4381 ctaatataca gtaagatacc tatggaattt aatgtaatat tagtatctat ttcaattgca
     4441 attttaacta tatatgctct tgctgtatct tatcatattg atgtaattgc attagtttct
     4501 ttatctttac tgatagtatc aaataatata ttttttataa atgtgcaatt gagctattta
     4561 gaaaataaaa tgtgtatatt taagacaagt attatagtaa gagtgatttt atggttaata
     4621 aaaactgtaa gggaggtcag taacgctata aagaaagtat ctttagcaag gaaagttatt
     4681 gttatagtga taatatcttt agctttaatc atggcaatta gcactcgaag tataggtatg
     4741 ggattactgt tttctatatg tattttttta ggatttacaa tatatataac taaaagactt
     4801 tcatatctta gttacgtaat agaaggtaca gaaagaataa aaaagggaga attagattat
     4861 aagataaaaa ttactgggaa tgataatttt acgtctttag ctgaaaatat aaataatatt
     4921 ggagaaggtc ttgatagagc tatagaagag caagtaaaaa gtgaaagaat gaagtctgac
     4981 cttataacaa atgtatctca tgatttgaaa actccactta cttctataat aaattatgta
     5041 gatttaatta aaaaagaaga atcaatacaa ccagagtata taaatgatta tataaatgta
     5101 cttgagtcaa agtcaaaaag actaaaatta ttgatagaag atttatttga agcgagtaga
     5161 gtaagtagtg gcaatataga attacaaatt agcaagattg atttagtgca actattaaga
     5221 caaagtatag gtgaacttga agaaaaatta tctaaaaata atttatattt aaaattaaat
     5281 gttccaaatg ataaggtgta tatatgggca gatggaagaa gaatgtatag agtatttgaa
     5341 aatcttttat ctaatatagc aaaatattcc ttagaagaaa ctagagtcta tattgatgta
     5401 tatgactatg gtgaaaatgt aaaggtaact atgaaaaata tatcttctta tgaattaaat
     5461 tttgacccaa gtgagattat ggaaagattt aaaagagctg atgaatcaag gaatactgaa
     5521 ggaagtggat tagggcttgc tattgcaaga gatttagtag ctttacaagg tggaaaattt
     5581 tatatagaaa tagatggaga tttatttaaa gcaaatttag agtttcaaaa atatgaagaa
     5641 gaaataacaa atatggaatg agatagtatt taagtatata tattttttta ctgtcgaaag
     5701 cttttattat aatatattag aatatttaca gtagagaatg aagagtaaaa acagagtaac
     5761 tttaagtcac tctgtttttt atttaaaatc aataatataa caacttaaat taaaattgta
     5821 cacatagttt taaaataact tatacaataa aacattatcc aaatatcaca agataattcc
     5881 aaataaatat atagatatca ctcctgtaat aatagaaata ccaatagatt taaacttata
     5941 tgcaacaaga agtacaatca taaaagatat aattttaggg ttagctatag ataaatatag
     6001 actatctcca ttaaaaaata catctttagc agttaaggaa gtaagtattg ctacaggtat
     6061 aaatttcatg atttttttca aaaaatcagg catctctttt tttgaaaaaa ttaacatagg
     6121 aagcactctt ggtagatatg taacaatgta cattccaatt ataataaaaa atatataatt
     6181 attattcata agattcctcc tttttacagt aagattcttc tataaagaaa ccaattaaag
     6241 atgctaaaat tgatgcaact attattacta aattgctttt gaataatgca tataaaataa
     6301 cagaaataat gattgatgat ataaatacta caatataaat cttatttttt atttgagaaa
     6361 ctattaaagc tataaacata gaagtcaata tgaagccaga aatagaactg tctatagata
     6421 tactttctcc taaaaatgct ccaataaaat tagctaaaac tgatgaaatt aagcagaaga
     6481 aattaagata aatagcattt ctatcagacc aatttccagc agtaaatttt tcataattaa
     6541 ctgcaaaagt ttcatctgtt atagtatgac aaaatggcaa tataaatttt ggacttttat
     6601 tttttatgta catgttcaaa ttagaactca ttagaaaatg tcttaagtta actataaatg
     6661 tagtcaaaac aatagatgtt acacttgcac cagtagaaat cattgaagct gcaatgaatt
     6721 gagaacttcc tccaaatact aaagttacca ttagtgctat ttgaaaaggt gaaagaccag
     6781 atttttgtaa tagtattcct cctactatac ctataggtat ataacctaag aatataggaa
     6841 ttgaagcaat aaagctttct tttaattgtt gagttctttc cacaataaat cactcctttt
     6901 gtattaaatg tattaaagta ttcgtaaaac agaaaaatag attaattgta taattgcaat
     6961 tataattaaa aataaatttg gattaatata taaaatacta tacaacaaat agataatctt
     7021 aaacggttat taaattttat ctatttgtta ttatgatgta ataatacata ttaatctcta
     7081 taagttaatt actatctggt gtaattttat tttaacaaca aaaaaaaata cacacaatta
     7141 aaatattgtg tgcaatacaa ttattttttc tcttctaata gtctttttaa tacgctcaaa
     7201 ccagctttaa gttcttctgt actttctgta gcacatacag ctattcttat tgcagaaatt
     7261 ggtttttctt ttccaacagc aaaacgttca gaagcataga cctgtacacc ttctttaagt
     7321 gcttgtatct caaatttttc tgccattatt ccatcaggta gaatcaacca tctaaatata
     7381 cattcttcat ttcccaaaag attatatcca gataaatatt gattaataat cttgtttctt
     7441 cttcttgcag atttacggcg tgcattaatt aatttatcgg cttcacctga tgtaatcatg
     7501 cgataagcaa tttctgttaa aaaataagat tgagatagat taatgttgta taatgcgctt
     7561 gataatgcct ctcgatactg tttaggtgta gagatatatg ctagccgtaa actaggtgca
     7621 ataatttttg ataaactcgt aatataaatt gtttgattgg gcagatagga agccactgga
     7681 tttaaatgtg tttcattaag taaactatga atagcatcct caataacaat taaattgtat
     7741 ttagatgcaa tatttgcaat catttttcgt ccattttgtg acattatatg tgtagttgga
     7801 ttttgataat ctggtattat gtatagacct ttaatatttt catttttaca agcatataat
     7861 agtccttctt cactaatctc attatcttct tgtttaattg gaattagttg aatcccaagc
     7921 attttagctg ctgtctttat tccaggataa gttaatgggt ctacacctat tctatctcca
     7981 tgttgaaaca aaccagctaa aatagcaaca attgcatttt gtccaccact tgcaggaagt
     8041 aagctatctg atgtagtttc aagcccagct ttataaatta atttggcaat agcttctttt
     8101 tgccatttag caccatctct ttgtccatat tggaatatgg ttttaaaatc tatttcctta
     8161 attatgtgtt taaacaaagt attcatttct tctagtgtga aatctggatt cattgtgccc
     8221 atttcaataa atgtaatttt attattactg gacatcaaga atagatttga acgtgtatca
     8281 taagatacaa aagttccact tcctgtaact ccacttatta gacctttttt ttcacaaatt
     8341 ttaaaagctc tagagatagt actcacatta acatctaaga aatctgccaa ttcacgttgg
     8401 ggtggtaatt tagtacctgg taataaaaat ccattgttga tatcttgttc tagttgttct
     8461 gcaagcgcct tatataatat ttgccctttt gaaggtctct ttggtttcca gttcatagga
     8521 taattttcaa aagaattaat tggcataagt tttcacctca cattattaat ttgatttttt
     8581 ctttatctaa aacaataaat tctaaagata caaataaaaa ctgataggaa ataattctat
     8641 cagtttttgt cttttaattg atttcaaatc ttcctataat ttttatacct tctttatcat
     8701 aaagagggat tgttttttta aatatattaa aatttctttt tcttgtatat aaaacagctt
     8761 cttctggtgt ctttggttga ttatcacaat cgtacaaatc tctttttttt acgtagccga
     8821 cttttttacc tctacctatt gtttgtataa gttctggttc aatggaattt ccatttgagt
     8881 ctattatatg tgtaccatag gtttctccat ttttattata attaatctta ccaatagtat
     8941 attctacatc ttcattttgt ttaaaattgg agttgataga aaatattgac cctattatta
     9001 taaaggttaa taaaaactta agacctaaat taattttctt tttatcttga ttaaacatag
     9061 aatctaacct cttttttaaa ataatatttt ttttatttag aaactgagat acgaatgatg
     9121 aatacttcaa agatttttga tactttattg tatctaatat tatatatgca tactctttaa
     9181 tttcactcgt ggtacacttg tcaatcacaa tttcatcaca agataattca cataattcat
     9241 gaaaatattt ttttaataag tataaaattg gattgaacca atatatgata caaattatac
     9301 taattagaag cttaaataag ttatcttttc ttttataatg aattaattca tgtttaaaga
     9361 tgaatcttaa ctctttatta ttgtactttt tttctggaat tattatttta cagcgtgtta
     9421 aacctataat ggcaggagaa taaatacctt taaccttata tatctgaagt tttttattta
     9481 tacttagttc ttttgcttgt tcatctaaga tactagtaat atctatttct aaaatttctt
     9541 ttttaaaatt ttctatgtaa ttataaaatt taaaatttgt tcttaaatag taaaaagaga
     9601 ttgctataat tcctaatatc cagatgtaca taaatatatc taaagtaatg ccctggtcat
     9661 atg
//
LOCUS       DAMJYX010000117         9446 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJYX010000117 DAMJYX010000000
VERSION     DAMJYX010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.122"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            86..1042
                     /locus_tag="PC479_003041"
     CDS             86..1042
                     /locus_tag="PC479_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2805565.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            1219..1374
                     /locus_tag="PC479_003042"
     CDS             1219..1374
                     /locus_tag="PC479_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805566.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1504..2517
                     /gene="aroF"
                     /locus_tag="PC479_003043"
     CDS             1504..2517
                     /gene="aroF"
                     /locus_tag="PC479_003043"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2805567.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2539..3603
                     /locus_tag="PC479_003044"
     CDS             2539..3603
                     /locus_tag="PC479_003044"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2805568.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3603..4916
                     /gene="aroA"
                     /locus_tag="PC479_003045"
     CDS             3603..4916
                     /gene="aroA"
                     /locus_tag="PC479_003045"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2805569.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4894..5973
                     /gene="aroC"
                     /locus_tag="PC479_003046"
     CDS             4894..5973
                     /gene="aroC"
                     /locus_tag="PC479_003046"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2805570.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            5976..7172
                     /gene="pheA"
                     /locus_tag="PC479_003047"
     CDS             5976..7172
                     /gene="pheA"
                     /locus_tag="PC479_003047"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2805571.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7219..8028
                     /gene="aroE"
                     /locus_tag="PC479_003048"
     CDS             7219..8028
                     /gene="aroE"
                     /locus_tag="PC479_003048"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2805572.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            8040..8573
                     /locus_tag="PC479_003049"
     CDS             8040..8573
                     /locus_tag="PC479_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2805573.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8594..9433
                     /locus_tag="PC479_003050"
     CDS             8594..9433
                     /locus_tag="PC479_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2805574.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         3889 a          915 c         1667 g         2975 t
ORIGIN      
        1 aataattatt aaaatagtgt aaaataatta cagtaaggat tataaaaaat ttttagaatg
       61 taaattgaaa tttaggagta gatgtatgta tatactgaat ttaaatacaa gagagactat
      121 tgaagatttt agagataaat tttatgtagc agaaaattct tatcttatat taagtgctcc
      181 aaaaaattta aaattgctta aggaaacttt ggatatagat gaaataactt ttaatgattg
      241 tcttaaattt gatgaaataa ccaaacttga tttatttgat aattacgatt ttttaagcct
      301 aaatacattt gaactaagag atggagaagc tgttatagaa gaggtaaata tgtatttatc
      361 agataatttt atattggttg tagtgaatga agaacacttt ttatttgaat ttgtaaaaaa
      421 tattattttg aagaactctc aacttgaaaa aaatccagtt attaatctat ttaagataaa
      481 ctacttgatt cttagagaag ttataaaaaa tggttttgag tctttggaaa aagttgaaga
      541 gttaattctt caaatagaag atgaaatgat ggacaacatt aataagaatc atgtatctag
      601 aataagtgat gtaagaggat taacaagaat tatagttaag aatactagac ctcttttgta
      661 tattggggat agaatagtta aggaaaatat aagatattta aaatattcaa atgtaaaaaa
      721 atataattta gaaaattttc aaggtataga ttttggaatt gacaaattat acaatttcgc
      781 attgtcaaca cgagagttag cagataaatt attagatatt tattcttcca gagtaggtga
      841 gaaaacaaat aacttaatta caaaattaac cttgttaaca gccatatcag caccacttac
      901 aattataact ggtatatatg gaatgaactt tagatatatg ccagaattaa actggattta
      961 tggatatcca gcaactttat tttttatgct atgtataatt tttgttggaa ttataatatt
     1021 taaaataaaa aaattattgt aaattacaga tgatgtttaa ttgattttat aaaaatacat
     1081 caattgagaa gctgatttaa gtaaataaaa aaatatttta aattgaaaat atttatagaa
     1141 aatattgaca attatatttt ctttgttata attattaaac aagaagaata ataaattata
     1201 aaaatggagg ataatactat gaaaggaata ttaaaagaac taacagcaaa actttatagc
     1261 tttttttact ttatgttctg gggcttttat tttagcgctg gctatttctt ttacaaaaat
     1321 atatgtactt cctttctggt gaggccttat tttagtgatt ttgagcataa gtaaatttta
     1381 taaaaatgat tattttatat atttatataa gatttagtgc ttgatagatt attttagaga
     1441 actcattagt gagttctctt ttttatttta aatataaaat tataatattg ggaggtagta
     1501 aaaatgatag tagtattaaa aatgggagca gataaaaatg aagttaaaaa attaattgag
     1561 gctattggga gagaaggagt agaagttaat cccatagatg gaacagaatt aactgtttta
     1621 ggtttagttg gagatactag taaaatagat gccaaaagaa tagaagcaaa taaggtagtt
     1681 gaaaaagtta tgcatgtggt agagcctttt aaaaaggcca atagaaaatt tcatccagaa
     1741 ccttcaatca taaatgttaa tggaatggaa attggttcaa agaaaattgc tatgatagct
     1801 gggccttgtt ctgttgaaac agaagaccaa atagtatcta tagctaaaga tgtgaaaaaa
     1861 tcaggtgcag gatttttgag aggtggagca ttcaaaccaa gaacatcacc atacgctttc
     1921 caaggattga aatatgatgg attggattta ttaaaaaaag caaaagaaaa aacagggctt
     1981 cctatagtta cagaaataat gtcaacacaa gatatagata tatttgaaga aaatgtagat
     2041 gtaattcaag ttggagctag aaacatgcaa aactttgatt tattaaaaga attaggtaaa
     2101 acaaataaaa caatactttt aaaaagagga ttatctgcta caattgaaga gtggctaatg
     2161 tcagcagagt atataatggc tggaggaaat gaaaatgtag ttctttgtga aagaggtata
     2221 agaacttttg aaacatatac tagaaatact ttggatttaa gtgcaatttt agctgtgaag
     2281 aaacttagtc atttaccagt tatagttgac ccaagtcatg cagcaggtaa atcatggatg
     2341 gttgactctt tatctaaggc agcaattgca gttggagcag atggtctaat aatagaagtt
     2401 cataatgacc cagcacatgc actttgtgat ggaaaacaat cgattaagcc aaatgaatat
     2461 gatgaattga taagtgaatt aaagactatc gcatcagcag ttggtagaga aatataatct
     2521 ataaaaggat ggttaaacgt ggaagaaaaa ctattagtaa atttaaaaga aaatagctac
     2581 aatatattta ttaaaaaaaa tatactaaag aatataggag aagaaataaa aaaaatatat
     2641 tttggaaata agatttttat tgtaactgat gaaaatatag caaaattata cttggataaa
     2701 gtaaaaaata atttaagtga atctggattt gaaattaaat ctttaatatt aaaatctggt
     2761 gaagaaacaa aatcatttaa tactcttcct aaaatatacg atagtttact tgactttaag
     2821 ttaactagaa aagatttgat tattacttta ggaggagggg taataggtga tttaggtgga
     2881 tttgcagcag caactttttt aagaggtata agttttattc aaattccaac atctttatta
     2941 gcacaagttg atagttctgt aggtggaaaa gttggagttg accttgagag aggcaaaaac
     3001 ttagtgggaa gcttttacca accaaaggct gtgtttatcg acccaaatgt attattatca
     3061 cttccagaaa aattctatag agatggaatg gcagaagtta taaaatatgg atgtataaaa
     3121 gacaaaactt ttttcaataa acttttaagt ttaaaaaata gagaagaagt aatgcaacat
     3181 atagaataca ttatacataa atgttgcttt attaaaaaaa ctgtagttga agctgatgaa
     3241 aaagactttg gagatagaat gcttctaaac tttggccata cattaggtca tgctattgag
     3301 aaatactaca attttacagg ttatacccat ggagaagctg ttgccatagg aatgtataag
     3361 ataactttag aaagtgaaaa gataggaatt acaaaaaaag gtacatcaga acttataaaa
     3421 tcaattttaa tgaattataa tttgccttat gagattgaat taagagataa taaatctata
     3481 gtagatgcta tatctcttga taaaaaaaat ctaggtgata cattaaaaat aatactttta
     3541 aaagatattg gagagagtat tatatataat actacttcta aattttttca ggaggtaaga
     3601 taatgggaag tctaaaaata tatccaagta aattatctgg agatgtaaaa ataccaccat
     3661 caaaaagtat ggcacatagg gcagttattt gttcatctct aagcaatgga aaaagtagaa
     3721 tttctaatat agatttttct gatgatataa tagctactat aagagcaatg acatcattag
     3781 gagctattat agaaaagaaa gaagatattt tagaaatttc gggaattttt tctaaagaag
     3841 gtattttaaa tagagagaat cagttgaatc aacctaaatt gacaatagat tgtaatgaat
     3901 caggctctac attgagattt ttagtaccta tatctttaac atttgatggt gtaaaaaggt
     3961 ttataggtag aggtaatctt ggaaaaagac cacttgatac ctattatgag atatttgaca
     4021 gacagaatat aaaatatagt tacaaagaaa atcagttaga tttgattata tcaggtaagc
     4081 ttaaaccaga tgaatttaga gtaaaaggaa atataagttc tcagtttatc acaggtttac
     4141 tgtttatatt gcctacgcta gaaagtgatt caaagataat aattactact gagctagaat
     4201 ctaaaggata tttagattta actctttcaa cgataaaaga ttttggggta gaaattataa
     4261 acaataacta taaagaattt attataaaag gaaatcaaac gtataaagcg agagattata
     4321 aagtagaagg agattattct caaggagcat tttatttatc agcagatgct ataggagaag
     4381 atattagcat attagacctt aaagaagatt ctcttcaagg ggatagtgaa gtagttgaaa
     4441 ttttatctag gatgggaatg gaaatattaa gagaaggcaa caaaataaaa ggaataacaa
     4501 atggtttaaa tggtactttg atagatgcat ctcaatgccc agatataata cctgtattat
     4561 ctgtagtagc atccttaagt atagggagga ctacaataat taatgctggt aggcttagaa
     4621 ttaaagaatg tgatagactt catgcaataa atgttgagtt aagtaaactt ggagcaaata
     4681 tagaggagaa agaggatagt ttgataatcg aaggagtaag caagttaaat ggtggagtag
     4741 aggtgtggag tcacaaagac catagaattg ctatgacact tgcaattgct tcttgtagat
     4801 gtgataaacc aataatatta aaagattttg agtgtgtatc aaaatcttat ccacattttt
     4861 ttaaagattt taagatgtta ggaggtcgaa ttgatgagtg gaatatgggg aaataattta
     4921 aaagtatcaa tttttggaga atctcatgga aatgctatag gtataaatat agatggtctt
     4981 ccttcgggga ttgaacttga ccttgataat attgataaag aaatgaagag aagagcacct
     5041 ggaaaaaatt ctatatctac ttcaagaaat gaaagtgata taccagaaat tctaagtgga
     5101 tattttaatg gaagaactac aggaacgcca ttatgtgcaa tcataagaaa tagtgatact
     5161 cgttcaaaag attatggaga gcttaaaaat ttaatgagac caggacatgc agatttcaca
     5221 ggtaatgtga gatattctgg atttaatgac tatagagggg gaggtcattt ttcaggtagg
     5281 ataacagctc cattagtatt ttgtggggct atatgtaaac aaattttatc acaaaaagga
     5341 atagagatag gagcacacat aaaaaaaatt aaaaatatag aagatatgag ttttgattat
     5401 gtaaatattt caaaacaaca gttatcaaat ttgcaaactt tggaactacc tttacttgat
     5461 ttatcaaagg aagaagccat gaaaaatacg attatagatg caaaaaatca aggagattca
     5521 gttggtggta taattgagtg tgcagtagta ggtattaatg tagggcttgg aaatccattt
     5581 tttgactctg ttgaatctac tttatcacat ttattgtttt ctgttcctgc tgtaaaaggt
     5641 gtagagtttg gtttaggttt tgaacttgca gatatgtatg ggtctcaaag taatgatgaa
     5701 atgtattatg aaggaaatca agttaagagc aaaacaaata acaatggagg aattatagga
     5761 gggattacta ctggaatgcc aataattttc aaagtggcaa ttaagccaac tccatcaatt
     5821 agcagacaac aaaacacagt taatataaaa gataaaaagg acgatatttt atatattaaa
     5881 ggtagacatg acccttgtat agttcaaagg gctatacctg taatagaagc agtaacagcc
     5941 attggaattt ttgatttgat gaaaggacga taataatgga ggatttaacg agatatagaa
     6001 atgagattga caatatagat aaagagctta ttcaattatt tgaaaagaga atgaatacag
     6061 ttcttgaaat tgccagacat aaaatgaaaa ataacactac tatacttcaa aaagatagag
     6121 aagaaaaagt actaagtaga gctgtagata atttagataa taaagcatat tcacaagaaa
     6181 cggtacagtt ttttaattct ataatggaaa ttagtagagg attacaaaaa agattgatag
     6241 ataatgatgg tgagcagaaa atcgagttta gtttagattc atcaaataag aaatttgatt
     6301 tgtcaaatgt aaatagatat aagtctttaa aagatgaatt aaataaaaaa aatatcctag
     6361 taggatttcc aggaaaatca ggttcattta cagaagaagc actaaataaa ttcttcaaca
     6421 aaaaaatatc aaaaaaacaa tttaaagaat ttgaagatgt tttcatagca cttaaaaata
     6481 aagaaataaa ctatggaata atccccatag aaaattcatc tacaggagct atctctgaaa
     6541 cttatgacct tttgagaaag tacggttttt atatagttgg agaagaatgt ataaaaatag
     6601 accaaaattt aatagggata aaagacacaa agcttgaaga tataaaagaa atatattctc
     6661 atccacaagg tataggacaa tgtagtaaat ttttaaaaca aaatagtaca tggaaattga
     6721 taccatttaa taacacagca acaagtgcag aacttgtaag aaaacttcaa gataaaacaa
     6781 aagcagcaat tgcaagcaaa aaagctgcaa gtatttatgg attagaaata ataagtcctt
     6841 gtataaatga tattgcaaac aactatacaa gatttgtagt aatcagtaat caaatacaca
     6901 ttgaagaaga atcaaacaaa atgagtgtag tattttctgt tgaacatgaa gcaggaaaac
     6961 tttataaagt acttggatac tttgctaaaa ataatataaa tatgacaaaa atagaatcaa
     7021 gacctatgaa aaatgcctca tggagatatt tcttttatat agattttgag tgttctatat
     7081 acaatgctaa agtgtacgat ttattagaat tgatagaaca taatacagca tattttaaat
     7141 ttatgggagt atataaaaat aaaactgaat agataagata aatttttatt taatttataa
     7201 ttattaggag gtgtaagtat gaattttttc ggtctagtag gtgaaaaact ctctcacagt
     7261 gtttcacctc aaatccacaa gagagtattc gagatattga atattgaaag tgcttataaa
     7321 aactttgaaa tttcaaaaga agatatatct aagctagacg gtgctataaa attattagga
     7381 atacaagggg taaatgttac tgtaccatat aaggagcgta ttatgaaata tcttgatttt
     7441 atatcaccag aggcaaaacg tataggagca gttaatacaa ttttacttag agaaaatatg
     7501 ttatatggtt acaatacaga ttactttggt cttgatagta tgtttaaaat ggctaatatt
     7561 gatgttcaag gaaaagtagc agttatatta ggaactggag gtgcttctaa agctgctctt
     7621 acatatttta ttgattctgg tatagaaaag ctttatgttt caactagaaa aaaggatgat
     7681 aaaaaattat taaatagtaa agctatatta atagattatg aagaacttaa acatataaaa
     7741 ggagatataa tattaaatgc tacaccagta ggtatgtacc ctaatgtagg aatttcacca
     7801 gtgtcaaaat ctattataca aaattttgat atattaatag atttaattta caatcctgga
     7861 gaaacagagt ttttaagaat tggtaatagt atgggtaaaa aaacttgtga tggattatat
     7921 atgctggtgg ggcaagcaat aaaatctcag gaaatttggc aagatactaa aatagataat
     7981 agtattttag atgttatata taatgaacta aaattagaat ttttgtaagg gagattgtta
     8041 tgataaataa aacaaaagaa aaattgatat taataggtat gccaggtagt ggaaaaacta
     8101 caattggtaa acttttggcc aaagaatata attgtagttt ctgtgatatg gatgattata
     8161 taattcaaat atctcaaaaa agtatagcag agttgttttc agaaggagaa gatatcttta
     8221 ggaactatga gactcaagca tgtagagaac tttctataag tgataaaaca gtgatatcta
     8281 caggtggagg tgtaataaaa aaggatgtta atatggaaat actaaaagaa acaggtataa
     8341 taatttttat tgatagacct atacaaaaga ttttggaaga tataaatatt aattcaagac
     8401 cattattaaa aaacggaaaa gatagattgt ataatcttta taatgagaga ataaatcttt
     8461 ataaaaaatt ttcagatata gagattttaa atgataaatc attaaataat gctgtatata
     8521 atattacaaa tgcagtcagt gaaaatttta aatttgactt taaagaaaaa taatctaggg
     8581 ggaaataaaa ataatgaata ttgttattgt aggactagga gttataggag gctcttttgc
     8641 taaagcatta aaaaaagctg gatatgaaaa tgtatttgga gtagatgtag atttagaaac
     8701 tttaaaaaaa gcagaaaaag caaaaatcat aaaaaaagga tgtactacag gtaaagagtt
     8761 atttaaaaaa gctgatttaa ttatattatc tatatatcct agattagttg tagattttct
     8821 aaataataat aaaaactttt ttaaaaaagg gactataatt actgatacta caggaataaa
     8881 agaaacactt atcaatgatg tgttgcaaat tattccagat gatatagatt ttatatttgg
     8941 acatccaatg gcaggtaggg aaaaaaaggg tattgatttt gcaagtgaac aagtttttaa
     9001 tggcgcaaat tatataatta ctccaactgg cagaaacaac ataaaaaatt tagagttagt
     9061 tgaaaattta attttagaga ttggatttaa aagagtaaaa aagttaactt cacaaaaaca
     9121 tgatgagatt atagctttta cttctcagtt acctcatgtt atggcagttg ctcttataaa
     9181 tagtgatgaa gaaggtagag atacgggtaa atttattgga gatagttata gagatttaac
     9241 tagaattgca aatatgaatg aagacttatg gagtgaactt tttttaggaa atagagataa
     9301 tttactaaaa gttatagaaa actttgaatc agaagtaaat ttagtaaaag aagctatatt
     9361 taacaatgat aaaaataaat taatagagta ttttaaaaaa tcatcaataa gaagagaagc
     9421 tttagaaaaa taaatattaa tattta
//
LOCUS       DAMJYX010000118         9301 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJYX010000118 DAMJYX010000000
VERSION     DAMJYX010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.123"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            344..1360
                     /gene="gap"
                     /locus_tag="PC479_003051"
     CDS             344..1360
                     /gene="gap"
                     /locus_tag="PC479_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2805575.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            1755..2924
                     /locus_tag="PC479_003052"
     CDS             1755..2924
                     /locus_tag="PC479_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805576.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            3304..7557
                     /locus_tag="PC479_003053"
     CDS             3304..7557
                     /locus_tag="PC479_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2805577.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            7706..8200
                     /locus_tag="PC479_003054"
     CDS             7706..8200
                     /locus_tag="PC479_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2805578.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            8316..8693
                     /locus_tag="PC479_003055"
     CDS             8316..8693
                     /locus_tag="PC479_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2805579.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            8899..>9301
                     /locus_tag="PC479_003056"
     CDS             8899..>9301
                     /locus_tag="PC479_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2805580.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLV"
BASE COUNT         3768 a          962 c         1666 g         2905 t
ORIGIN      
        1 atattttttg aactaaataa aaatttattt ttattatttt aaattagagg agattcttga
       61 agatatatta tttttaaaaa taagtatagt ttaaaaattc aaaaaaaaga tggcaaaata
      121 tgtcccaagg gacattgagt aaaataaatg atttcagctt atttacacta aaaagtacat
      181 actaaatgag gggtaaaata aagaaaaatg atatactaat tgtcttttga taataaaaaa
      241 acatggaatt tgattattta atcaagaaaa agtattgaac aaatgattta tttagtgtac
      301 aataatcatg taaaaacata gtaatggagg gagacatttc ataatgaaaa ttaaggtagg
      361 aatgaacgga tttggaagaa taggaagagc tgtattaaga atagctcaag aagaattagg
      421 agataatata gaaattgtag ccataaatgc aagagcaaca acagaatctt tggcacattt
      481 atttacatat gattcatgct atggaacttt tagaggtgaa gtagaggcaa aggatgaaga
      541 cactctagta gtaaactcta aagagataaa aatattaaga tataatgacc cagaagaatt
      601 accatggaaa gaactggggg ttgatatagt aatagaatca acaggattat ttacacaaag
      661 agaaaaggca gaaaagcata ttaaagcagg agcaaaaaaa gtaattataa cagctccagg
      721 aaaaaatgaa gatataacta tagttatcgg agttaatgag aaagaatatg ataatgaaaa
      781 gcacaatata atttcaaatg catcttgtac tacaaattgt ctagctcctt ttgctaaagt
      841 attggacgaa aaatttggta tagtaaaagg gttaatgact acagtacatt catatactaa
      901 cgatcaaaga atattagata aaagccataa agatttgaga agagcaagag cagcggctga
      961 atctataata cctaccacta caggagcagc aaaagctgtg tcaagagtac taccacaatt
     1021 agaaggaaaa ttaaatggtt tttcacttag agtaccaaca ccaacagttt cacttgttga
     1081 tttagtgtgc gaacttaaag aaaacgttac atctgagcaa gttaactctg tgttaaaaga
     1141 tgctgctgaa ggagaattaa aaggtgtttt aggatattgt gacaagccac ttgtttctat
     1201 agattataaa ggagattcaa gatcatctat tatagacgct ctatccacaa tggttataga
     1261 agacaatatg gttaaggtag tttcttggta tgataatgaa tggggatatt cttcaagaac
     1321 tgttgactta gtaaaatatg tagctgaaaa attaaaataa tatatatttc ttaaagcaaa
     1381 ttttaaaaaa attttttaaa aaaattatat aaaactaaaa aaaaagatta tgaaaaatat
     1441 tgaaggaaaa attttttcat aatctttttt ctttcatgca aaaagataca agttacaact
     1501 tatatttgca tattaggtcg ttagagaagt atatatatat ttagtaagga atcatacttt
     1561 ttataaaatg aaagataaga tgtcgaaaac ttgcataaat ctgaaaaatt tttattatta
     1621 aaatactgat ttccatttta aaaatgataa aaaaatgaca aataaagttg caaatagtaa
     1681 tacttataag tataattaaa aaagagaaaa ggttttgtta aactttataa caaaaaaaga
     1741 agggatgatt caaaatggag aagttaacag acaggagaca ggttaaggca gtaagcatgg
     1801 aaaccttcat tttcctaata ttactagtag ttggttttgg gtatgttggt agtattatgg
     1861 gagcaggaat gatgttcaaa gtaataatga gtacagcaca tgctttacta ttggagacag
     1921 ttttcttaat tatggctatg gcagttcttg caggcgcatt aagcgcacta ctttcagagt
     1981 ttggagtaat agcgttaatt aataaaattt tcgctgtatt tatgaagcct ttatatggac
     2041 tacctggtgc tagtatagca ggagctataa caacttattt atcagataat ccagctatta
     2101 taccttttgc taaagataaa acattcacac agtattttaa acaataccaa gttccagcat
     2161 tatgtaactt aggaacagcc tttggtatgg gacttatctt aacaacattt atgatttctc
     2221 aaggtacaga atatgtactt ccagcattaa ttggtaattt aggtgcaatt ataggtagtg
     2281 tcataagtgt tagaataatg ttgacattta ctaagaagtt ttataaatac agtccggaag
     2341 aagataaagc aacaggaaca ttagagaaaa aggatgaatt tagagaaatc agagaaggaa
     2401 atgtatttca aagagcttta gatgcaattc ttgaaggtgg taaaatgggg gtagatatgg
     2461 gaatggctat aattcctgga gtcctagtag tatgtacatt ggtgatgtta ttaacttttg
     2521 gaccatctac agaccctgtt acaggtcaag aagtttatac aggagctgca tatgaaggaa
     2581 taaaattact tccagttatt ggagataaac ttggatttat attagaacca ttatttggat
     2641 ttacatcacc agaggcaata gcattcccta taacttcatt gggagctgtt ggcgcagcaa
     2701 tgtctctagt tccagagttt ataaagtcag gagctataac tcctaatgat atagcagtat
     2761 ttacagctat gggtatgtgt tggagtggat atttaagtac acatattggt atgatggatg
     2821 cattaaatgc tagacagttg gcaggtaaag ctattctttc tcatacaata ggtggtttat
     2881 gtgcaggagc agctgctcac tttatattta cactcgtagg ataaatgtaa tcagctaaga
     2941 taaaaaataa tatacaaaaa atggttgttg atatgtaata agaatttaca tatcaacaac
     3001 catttttttc tgactaaaga taatttataa tacataaaca ttaagtggat attagagtta
     3061 agttatattt atgtaaaaag taacaaaaat ctgtagaaat ttatgacaaa atttagtaca
     3121 ctaactatat gttcttattt aaaatttaag gttagaatta ggaattacct agtagagtaa
     3181 gctttgatta ataaaagaat cataaaataa aaatattagt cataaagatt ataaataact
     3241 tacttaaaat aaagccatag aacttacaaa aattaaaaaa gcatataaat atggaggggt
     3301 acaatggatg aattatttca tataggaatt gatgtaggtt ctacaacggt gaaggttgtg
     3361 gtacttaata atagtaataa tatagttcat aaagaatata gaagacatta ttctgatgta
     3421 aaaaaatcag ttaaagaagt actaaatggt atatatgaaa aattgggaga tataaatact
     3481 accattataa taactggttc aggtggaatt ggaatatcaa aaaaattagg tgtaaaattt
     3541 gttcaggaag ttatatctag tacaaaatca atagagtatt ttcatcctga gactgatgtt
     3601 gtcatagaac ttgggggtga agatgcaaag ataacatatc taagtggagg aatagaccaa
     3661 agaatgaatg gaatatgtgc aggtggcact ggagcattca tagaccagat ggcatcactc
     3721 ttaaagacag atgccagtgg attaaatgaa cttgctaaag ggtataatgt tatatatccc
     3781 atagcttcaa gatgtggtgt ttttgctaaa acagatattc aacctcttat aaatgatgga
     3841 gccaatcaga ctgatatagc aatgagtatt ttcaatgcag ttgtcgtaca aactgtgagt
     3901 gtcctttctt gtggaagaaa aatagaagga aatgttgcgt ttttgggtgg tccattgtac
     3961 tttttatctg aacttagaga agcatttaaa agagtattag atttaaaaga tgaaaacata
     4021 atcttcccag aaaatgcaca actttatatt gctattggag caggattatt atcagtagat
     4081 gaagataata tacaactaaa atctctaata gaaaaattgg atagtataaa gaatattgaa
     4141 gatggagaag taaatttatt agaaccttta tttaaagata aaaatgagta tgaagaattt
     4201 tcaagaagac atgaaaaaga aaaaataaat tatgtggaca taaattcaat aaaaagcaat
     4261 tgttatttag gaattgatgc aggctcaact acaactaaag cagcacttat tgatgaggat
     4321 ggaagacttg tatattctta ttataatagc aatgaaggaa atccattaaa aacaaccatt
     4381 aaagttataa atgaagtata tgatatatta ccaaaaaata ttaaaatatt gagttcaact
     4441 gtaactggat atggagaagg tcttattaaa aaagctctta aaatcgataa tggagagata
     4501 gagactatag cacattacaa agctgctaaa tttttcaaca aagatgtaga ttttatactt
     4561 gatattggtg gtcaagatat gaagtgccta aaaataaaag atggagtaat tgataatata
     4621 atattaaatg aggcttgttc atcaggttgt ggctcatttt tagagacatt tgcaagttct
     4681 ttatctatga caatagaaga atttgcgcat gaaggtattt attctcaaaa tcctgtagat
     4741 ttaggttcaa gatgtactgt atttatgaat tctagagtaa aacaagctca aaaagaaggt
     4801 gcaagtgttg gagatatatc agcagggcta tcttattcag taataaaaaa tgctttattt
     4861 aaagtaataa agattagaga tttaaatgag attggtaata atattgttgt acaaggtgga
     4921 accttttata atgatttggt gcttagaagc tttgaaaaac taattggaaa aaatgtaatt
     4981 agacctaata tttcaggaat tatgggagct tttggttcag cacttatagc taaagaaaaa
     5041 tatgaaagtg gatacaaaac atccttgtta tctaaggaag agttgaataa tatcaagcta
     5101 gaagcaagtg taactagatg taaaggttgt tcaaatcatt gtcttcttac aataaataaa
     5161 ttttctgata atgaaatttt tatttcaggt aatagatgtg agaagggtga agctatttat
     5221 ggtgataaaa aaataggatt agaaaagaaa aaaccaataa atttatttaa gtacaaatac
     5281 aatagaattt ttagatacaa accattggaa aaagaagaag caaaaaatgg ggaaataggg
     5341 ataccaagag tattaaatat gtatgaagat tatccatttt ggtttacttt tttcaatgct
     5401 atgggcttta gagttgtact ttcagatagg tcatcaaaac agttatatga aactggtata
     5461 actagtattg catctgaaac agtttgttat ccaggaaaat tagttcatgg acatatagaa
     5521 aatttaattg caaaaggtat aaaaaatata ttttatccaa gtgtaacaaa tgaaaataaa
     5581 gaggattcaa atgcagataa ttattataat tgtcctgttg taatatcata ttctgaagta
     5641 ataaaaaaca atgtagagaa tataagaaat aagaacataa attatataaa tccatttata
     5701 agtctaaatg ataaggaaaa attgaagaaa agactctatg atgaattatc aagacatttt
     5761 tctgatataa aaataactaa agaagaagtt aatcatgcag ttgataaagc tactgatgaa
     5821 cagaatttat ttaaaatgga gatacaagca gcaggtgaaa gagcacttaa agaaataaaa
     5881 gaaagaaata tgaaggggat tgtgttatgt ggaagaccat atcatataga cccagaaata
     5941 aatcatggaa tgccagagct tataaattca ttagatatgg cagtgcttac tgaagactct
     6001 atatgtcatt tagccaatgt acaaagacca cttagagttg tagaccaatg ggtatatcat
     6061 tcaagattgt ataaagcagc tagttttgta agagataaaa actatttgga acttgttcaa
     6121 cttaattcat ttggctgtgg attagatgca gtaactacag accaggttca agaaatactt
     6181 aatgaaaaat cgaagatata tactattata aagattgacg aaggcaacaa tttaggagca
     6241 gctaagataa ggatgaggtc cttaaaagca gctatgtttg agagagaaag caaaaatata
     6301 gatgtaaaaa atataaaggt aaacaggata cagtatgcta aaaataatca gataacgaac
     6361 aaacatacta tattagcacc acaaatgtct ccaatacatt tccaattcct tgaaaaagct
     6421 gttaacttaa gtggttataa tatagaaatt ctaaaagata ctgatagtag tgtaattgaa
     6481 gaagggttaa gatatgtaaa taatgatgca tgttatccag ctataatagt tgtaggtcag
     6541 ctaataacag cattaaaatc tggcaaatat gatttaaata acacttctgt tactataact
     6601 cagactggtg gaggttgtag agctacaaat tatattggat tcttaagaaa ggctatatac
     6661 gatgctggat ttaaagatgt tcctgtcatt gcacttagtg taaatggaat agaagatagt
     6721 ggtataatgg ataatatatc actaaaatta atcaatagac tctttatgtc tgttgtttat
     6781 ggtgatttgc tgatgaaagt actatataga gttagaccat atgaaaaagt tactggaagt
     6841 gcaaatgctc tatatgaaaa atgggttgat atatgcaaat cttctcttgt taaagcaaaa
     6901 atttctgtat ttactagaaa tataaaagat atagtaaaag aatttgataa tttagagata
     6961 ctagatatca aaaaaccaaa agtaggtttg gttggagaaa tattggtgaa attccatcca
     7021 attgcaaata ataatttagt agatatatta gagcgagaag gtgctgaagc agtcgtgcca
     7081 gaccttacaa atttcttctt aagttgtgcc tttaatacta tatacaaaca cactcattta
     7141 gaaggaagta gaaagagtag aatgatagga gaggcattta tatacataac tggaatatat
     7201 caaagagtat ataaaaaagc acttgataag agtgagagat tttatgctcc agccaatata
     7261 aaaaatgttg ctaaaagtac agaaccagta gtgtcattag gtaatcaaac tggagaaggt
     7321 tggcttttaa caggtgaaat ggttgaatta ttaaatgaag gtgtagaaaa cattatttgt
     7381 atgcaacctt ttggatgttt acctaatcac ataattggta agggttctat aaaagaatta
     7441 aagaggttgt ataaaaatgc aaatataata cctattgatt atgacccttc tgcaagtgag
     7501 gtaaatcaac ttaacaggat aaaacttatg ctttctaagg cttttaaaaa tatataagat
     7561 ttatattttg atatgttaaa agttaatgaa tttaaaaata tactctatat taagtatcta
     7621 taaaaaagag ttatcctatt ttttagggta actctttatt agttaaaaaa ttatatgtat
     7681 actgatatta aaggggggaa gtaatatgag tttagtatat gatgtttata aatcaaaatt
     7741 aggaaatttg tacatattaa gtgaagatgg ttttatagtt agtatataca taggagatga
     7801 aaaatttaat aatttaaaaa atgaatgtat taaaaaagga attgatataa agagaagtaa
     7861 ggaagattta aaggaagcaa taagccagat agatgattac tttaatggaa aaagaaaagt
     7921 ctttaattta aatattcgta ttgaagggac tgaatttcaa aaatcagttt atagggaaat
     7981 gttaaaaata ccttatggag aaacattgtg ttattcagat atagcaagaa atataaacaa
     8041 tccaaaatca gtaagagcaa taggacaagc aagtaaaagt aataaaattc caataataat
     8101 accttgtcat agagtagttg gcaaaaaaga tattggtgga tatatgggga atcattctga
     8161 tttgaaagag atactattaa atttagaaag acagagttaa cttagaaaga aatgttttta
     8221 ttgttaatat ttgatataat aatagtaaaa agaagtattt tttacaaaaa caatgaagaa
     8281 tttattttaa tttacaaaaa tgagggggaa ttttaatgtg tgataaagtt atagcatcaa
     8341 aatatcatga gaagggattt aattgtgcag aatcagttat aaaagcatat aatgaggaat
     8401 ttaatacaga tataccagtt tgtttaggaa gtggactggg ttcagggtgt ggggttgcta
     8461 gtttatgtgg tgcagtaaat gcatcaaata taataattgg atatgtaaaa ggcagaaacc
     8521 acgaagatga aagtactaaa gctaaagtat atgctaaaga tttaactaca acagtaagaa
     8581 aagagtatgg ttcagagtta tgtatagacc taaaaaaaga tttagttgct tgtagagaaa
     8641 taatggattt cgcatatgat agtctaaagg aaactttaaa aaaagaatta taagaaaata
     8701 agctatcaaa tataaattat ttgatagctt atttttatag ccgatactaa ataaatcaaa
     8761 tatattttag aattattatt tgtaaataaa cagcaaataa ttttaggctt tttatttaat
     8821 atagttttta tttatattta tttttgaata tgttatactt tgtttaaata aatatttgta
     8881 atgagggaga tgagaagatt gaagaatctc aatttactta ttaataaatt atattcaaaa
     8941 aatcataatg aagcatataa aactttttta ttcttagaaa atgaaagttt aaaatcaaat
     9001 attacatatt gtttttttga ctcattccta gaaatgattg ataacgaaaa ctcatatatt
     9061 cgaacaaggg gattgttatt aatctctgca aatgctcagt gggatattga taataaaatt
     9121 gaaataaata tagatagtat actaagtcat atcgtagata aaaaaccttc tgtatcaaga
     9181 atgtttatta aatccatacc taatattaca aagtataaag aaaatcttat taatcgcata
     9241 aaaatggaat taagtaatgc agatataagt atttataata ataatatgaa accattagta
     9301 g
//
LOCUS       DAMJYX010000119         9192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJYX010000119 DAMJYX010000000
VERSION     DAMJYX010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.124"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            393..1598
                     /locus_tag="PC479_003057"
     CDS             393..1598
                     /locus_tag="PC479_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2805581.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1669..1890)
                     /locus_tag="PC479_003058"
     CDS             complement(1669..1890)
                     /locus_tag="PC479_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805582.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC479_003059"
     CDS             complement(2031..2630)
                     /gene="recR"
                     /locus_tag="PC479_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2805583.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2643..2993)
                     /locus_tag="PC479_003060"
     CDS             complement(2643..2993)
                     /locus_tag="PC479_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2805584.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC479_003061"
     CDS             complement(3052..4689)
                     /gene="dnaX"
                     /locus_tag="PC479_003061"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2805585.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4766..5030)
                     /gene="ffs"
                     /locus_tag="PC479_003062"
     ncRNA           complement(4766..5030)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC479_003062"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5068..5159)
                     /locus_tag="PC479_003063"
     tRNA            complement(5068..5159)
                     /locus_tag="PC479_003063"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5123..5125),aa:Ser,seq:gga)
     gene            complement(5160..5615)
                     /locus_tag="PC479_003064"
     CDS             complement(5160..5615)
                     /locus_tag="PC479_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2805586.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC479_003065"
     CDS             complement(5936..7207)
                     /gene="serS"
                     /locus_tag="PC479_003065"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2805587.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7333..7538)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7666..8547)
                     /locus_tag="PC479_003066"
     CDS             complement(7666..8547)
                     /locus_tag="PC479_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2805588.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8559..9068)
                     /locus_tag="PC479_003067"
     CDS             complement(8559..9068)
                     /locus_tag="PC479_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2805589.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2943 a         1599 c          973 g         3677 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaataa aatccctata
     1621 gtatatatat tatctattag ggatttttta ttttctaatt atttaatatt aaattggttc
     1681 ataaaagtct tcttcataat catactgata atttttataa ttatttctaa attcagcatt
     1741 tttatttcgt acattttttt tagttttttt cttattagaa aaacttatat cctcaaaatc
     1801 atcatattta ggtttatctt ttgatttcat ttttttaact cttttttctt tcattgcatt
     1861 atgttcttct aagttcataa ttggtttcat gttgcacccc tatcaaaatt tttaaatgat
     1921 ttatacaaca aattatacaa tacagaagaa atttattcaa taaatctaaa tcaaaattaa
     1981 tttgaattta actcaaaaaa ttacaaaaat cgaggtatta acctcgattt ttatatttct
     2041 cttctgcctt ctagtgcttt tgatattgtg acctcatctg catactctaa atcgcctcca
     2101 acaggaagac catgagctat ccgagtgact ttgattccca ttggttttac taacctagca
     2161 atatacatgg ctgtagcttc accttcaata gtaggatttg ttgccattat tatttctttg
     2221 acttcctgat tgccaagtct ttggataagt tcctttattt ttaacatatc tggtccaatt
     2281 ccatccattg gagaaattac tccatttaat acatggtact gtcctttaaa ctctctcgtt
     2341 ctctccatag ctgctacatc tcttggatct tccacaacac atataacact tgaatctcta
     2401 cttttatttg aacacatgct acatgggtct gtatctgtta tattacaaca aattgagcaa
     2461 tatcttattt ctctttttgc atctataatc gctttagata aagcttccac atcattagta
     2521 ttcatattaa taatatgaaa agccaatctt tgagcagtct ttcttcctac tccaggaagc
     2581 tttgaaaatt cctctataag tctcgttatg ggacctgtat aaacttgcat aaataccacc
     2641 tactagaata acccaggtat attcatacct ccagttacct tactcatttg acttgcttgt
     2701 atatcatcaa tatttctcaa tgcttgattt actgcactta aaactaaatc ttgtagcatt
     2761 tctatatcat ctggatcaac tacttctggc ttgatagtta tatctataac ttctttttta
     2821 ccattaactt ttacagtaac tgctcctcca ccaactgaag cttctacttc tttactttct
     2881 aactcttgtt gagctttttg catattttct tgcatttttt gagcctgctt taacaagttg
     2941 ttcatatttc caccaccagg catcattccg cctccaaaac cttttttagc cataaacact
     3001 attcctccta aacttatttt cattttatta ttatatcata atagtatttt attatttact
     3061 ctcattttca tcaatactat cttttatttc taatatctct tcactcacta tgttctttaa
     3121 tatttcctca ccaatgtcct ttttctcttt aacttcaaga tttatatctt taacttctga
     3181 tgtcagtact atttttacat taaaacttct atttaaaaca tctcttattg ttttttctaa
     3241 ataatttatt gtatcttgag agctcagtct agtttttgca aaatcaaatt tatcatcaaa
     3301 tataacatac aacatattag aatatacatt aaaacttttt acttcactta agagtgcgta
     3361 tattggcatt tttttatctt ttttaatttg tttaagtata ttcttccaag atgactctat
     3421 aagtataacg tcctcatttt ttacatcttc ataaactaca ttttcttgtt ttacatctat
     3481 ttctctatcg acctcatact ctttttcttt attattccca attttttcag acttaaagtt
     3541 ccccagttca atcatttttt ctaagttctc aactcttttt ataagagctt ctttactttc
     3601 atcaaacatt ggttgagcaa ttttcattat ggttatttcc actaaaaccc ttggatttga
     3661 agatgacttt atatcatcct gagttattga taatatattt aaaatcctta ttaaatcatt
     3721 tatatctata tttttagact gaattttaag ttgttctata gtttcttctg gtaatgaaat
     3781 tatttcatcc aattctgatg aaaccttaca taccattaaa tttctaaaat gatcaattaa
     3841 gtcatttata agatttctta tatctttgcc ccataagaca aattcattta atatctcaag
     3901 agacttttta gtgtcctgct ctactataca ttgagacaat tcaaataatt gttctatatt
     3961 tacgcttccc attaattcaa caacatcttt ataatctatc ttactttctc caaaagacat
     4021 acattggtct aatatactaa gtgcatctct aagagctcct tgagaatttc tagctattaa
     4081 attaagagct ttatcatcta tatcaattcc ttcatcttca caaatctttt tcattctaag
     4141 ggtcatatct ttaactgtaa ctcttttaaa atcgtatctt tggcatcttg ataaaatggt
     4201 tgcaggtatt ttgtgaggct ctgtagttgc taatataaaa attacatatg atggaggttc
     4261 ctctaaggtc tttaataatg cattaaaagc tccttgagac aacatatgaa cttcatctat
     4321 tatatatact ttatatttag cattggctgg agaatatttc acactttctc taagctctct
     4381 tatatcatct acactattat ttgaagctgc atctatttct acaacatcca ttgtattatc
     4441 ttttaataca gattcgcata tctcacactc attacacggt tcttcattta ttttattcaa
     4501 acagtttact gctcttgaaa aaatctttgc agtagatgtt ttaccagtac ctcttgttcc
     4561 acaaaacaaa taagcatgtc ctatattatc actatatatt tgattcttta aagtcttaat
     4621 aatatgatct tgaccaataa catcttcaaa tttttgtggt ctatatgctc tatataatgc
     4681 tttatgcatc tttttacctc tctttagcaa aattaataaa aaaatccctc attatctaag
     4741 ggattagtct tttatatatt aaatgctgta caccatctat cgacttaatt ccataggcgt
     4801 taccaaaaca gttagctcaa cttaggcacc cccacggcac acaaaagtga tcacttaccg
     4861 ctgcttcctt ccggatctga cggggttcat gagctttcat tgcgcaggac ctaagcatca
     4921 acaccactta tttagggcgg ccctacagaa aataagcctt tagatggaat tcaatcctgc
     4981 tatagcggat tgcaggttac agggcaccgc tacctcccca tctagtacag caaaattaca
     5041 accatattta attactttca ttgtttctgg cggagagagt gggatttgaa cccacgatac
     5101 agttttaccc atatactcgc tttccaggcg agcgccttcg accactcgac catctctcct
     5161 taatctcttt ttctcaatgc cttaaaaaat tcttgcaata tgatcgagca ttcttcttgc
     5221 aaaacatcaa aagctacttt tacattatta ttattaaaat aatctaattt aaactcatgc
     5281 tgtttttcta tatatgaatt ttttacatgt cttgtcccta ttataagctt ttttattcta
     5341 gaattaacta ttgcgccact acacattatg caaggttcca ttgtaacata taaatcacaa
     5401 tcagtcaacc tccaaccacc taatttttct gatgcttgtt ttatcgctaa aatttctgca
     5461 tgtgccgtag agtcttttaa cgtttctgta aggttatgag ctctagctat tatttgatta
     5521 tctttaacta taatagcacc tattggtgtt tcttttttat tatatgcttt atatgcttct
     5581 tttaatgctt ctttcatata aaatgaactt tccataattc ctcccaaaac ttataacaaa
     5641 cttatcttat cataattcat tttataattc aacttaattt tattttgttt atttaattat
     5701 tactatatat agctatattt ttttaaaatt agttctaaaa taatttttag atattcatac
     5761 aagagtgttt tataaaaata tttagtgttt tatgatgaaa attctgctgt gtaaattaac
     5821 ataaaaaaac tatgaaatta atatttataa taatttcata gtttttttat ttattgttta
     5881 ctcataatat attctcttca tcaaagcata ctctatatat acaattaata taaatttatt
     5941 ttataacatc aacacccata taaggtctta attcttctgg aactactaca gaaccatctg
     6001 cctgctgata attttctaat atagcagcca aacatcttcc tattgctaat ccagaaccat
     6061 ttaatgtatg aacatattct gcttttgatt ttttatctct tttaaatctt attcctgctc
     6121 ttcttgcttg gaaatcttca aaatttgaac aagaagatat ttctacatat ctattgtaac
     6181 ttggcatcca aacttctaaa tcatatttaa atgcagctgt aaatcccaaa tctcctgtac
     6241 atatttttac aactctatat ggaagaccta acatttgtaa catagtttca gcatcatgag
     6301 ttaatgattc taattcatta taagaatctt ctggtttaac aaatttaact aattcaactt
     6361 tattaaattg atgttgtcta actaaacctc ttgtatctct tcctgctgaa cctgcttcag
     6421 atctaaaaca aggagtatat gcacagtatt taattggaag ctctgcaaca tctaatattt
     6481 cattagcatg tatattagta acaggaactt cagctgttgg tattaagaaa tactctaatc
     6541 cttctatttt aaacatatct tcttcaaatt taggaagttg accagtccct ataaaactat
     6601 ttctattggc cataaatgga ggtaagactt ccgtatagcc atgcttagct gtatgagtat
     6661 ttaaaaagaa attcattaaa gatctttcta atctagctcc taatccccta tataaagtaa
     6721 accttgaccc agttattttc cctgctcttt caaaatctaa aatacctaga cctgtaccta
     6781 tatcccaatg cgctttatgc tcaaaatcaa atttagtagg ttctccccaa gttcttattt
     6841 gaacattgtc ttcatctgtt tcaccttgtg gtacctctgg atgaggaaca tttggtattc
     6901 tcattaatgt atattccatt tttgcttcta cttcttttac tttctcatca atacctttta
     6961 tcttatctga taactctttc aatcttgcct tttcttctgt tacatctttt ccttctttta
     7021 ttaattgagg tattttttta gaatttacat ttaactcatt tttcataact tcaacttctt
     7081 gaagtatttc tcttctttta ttatctaatt ccactacagc atcaaggtca aattctcttt
     7141 cacctcttct ttccatagct tttttgatat catctagatt ttctcttatt cttttaatat
     7201 ctaacatatt aattcctcct aataattttg ataatataag ttataattcc aacttaattt
     7261 acaaacatta tacttgtaaa ctcttagttt aaacaaattt taaaaataaa aaaagacctc
     7321 cgtttaccct ataaaaggga cgaaaatctt ttatccgcgt tgccacccta attgactata
     7381 ctaaaatgta taatcctctc agacctataa cgtaggtaaa cgtctagctt taactagaag
     7441 ctctagagta gattcaaaaa ccattgtaat gagttttcac catccactca ttctctgaat
     7501 acaatctgtt ttttactatt ttccttcaca gctttttaac taattgtttt atttgataat
     7561 cattatatta tatatattaa catctttcaa tactattatt caaatttttt tagaattttc
     7621 tttaattttt tatattaaat tcattttttc tatttaatta aaaccttatc ttttatccat
     7681 tgtatttaga ttacctgtat tattaaataa taatgaagaa ggaacaaatg gaacttttat
     7741 atcattctca tcaaatttat tttttacctt ttccattaca tcaaaatata catcccagta
     7801 atcatcagtt tttacccaag gtctagcaac tatttgcatg gtgttatttc ctatagagtt
     7861 taaacctatt ataggctttg gatcatttaa tattcttttt tcgtttgtaa aaatttcatt
     7921 taatatagac ttaactacat ctatatcact atcatatgaa atattatatg aaaaatctat
     7981 tcttcttttg ttttgatgtg tgtaattaat tatattatta cttgtcagtt gaaaattagg
     8041 tataattata gttttattat ctggcgtctt aagaacagtg ctaaatattt gtatgtcaga
     8101 aactgtacct tctacacctg aaccttgaat ataatcacct atattaaatg gcttaaaaaa
     8161 taaaataatc ataccagagc ctaaatttga taatatttct ttaaaagcta aacctatact
     8221 aaatccagca gctcctaaca cagctacaac tgatgtagtt tgtatcccta taatacctaa
     8281 gcttatcaat gatataatag ataacataga tatatatatt ccatatacaa taaaattatt
     8341 tacaccttta ctcattccat atttgattag aaactttgaa accatccttc ttactatttt
     8401 agatatataa atacctatca caaatacaat tattgcatat ataatatttg gtatattatc
     8461 actaaaatta tcgagcatat tttctaaagt tctcttagtt atattgctat ttgttgaggc
     8521 agtcttagcc aatgataaaa aattcattca actcctcctt atgataatat tccgttgtcc
     8581 tttaactctt ttactatagc ttctatagtt tcttctttta ttgcctcaat tgtatgaata
     8641 tggctacatt ctgtcagaac cgataattgt ttaaattcat ttgtctcaac tttcttcata
     8701 aagttgtcta tatccatttt agaataaata ttaagttctg cagttatctg accatatgtt
     8761 ggatgattta cgatgacatc ttttataata ccacctaaat ccacaattat ctttaattca
     8821 tcataaattt ctttagaatc cttatgattt ttacacttta tttgttttgt aaaatacggt
     8881 tgattatata ttatatatcc ttgaggtgta gctattattt caaatcctct agctcttata
     8941 agggctatat cttgaactat tacttgccta cttacattta atagttcagc tagtgttcca
     9001 ccttttacag gatgcttaga ttcttttaaa atatctatta atttacttct tctttcatta
     9061 gaattcatat tacaccttct ttccaaataa aaactttacc tatactatat aattttatat
     9121 tttatcttat tataaatcaa tattgacttt atattctatt taatcttaac ttttataata
     9181 aataaaaaag ac
//
LOCUS       DAMJYX010000120         9061 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJYX010000120 DAMJYX010000000
VERSION     DAMJYX010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.125"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            94..414
                     /locus_tag="PC479_003068"
     CDS             94..414
                     /locus_tag="PC479_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2805590.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            552..1196
                     /gene="trmB"
                     /locus_tag="PC479_003069"
     CDS             552..1196
                     /gene="trmB"
                     /locus_tag="PC479_003069"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2805591.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1325..2071
                     /locus_tag="PC479_003070"
     CDS             1325..2071
                     /locus_tag="PC479_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805592.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2200..2478)
                     /locus_tag="PC479_003071"
     CDS             complement(2200..2478)
                     /locus_tag="PC479_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805593.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2753..3199
                     /locus_tag="PC479_003072"
     CDS             2753..3199
                     /locus_tag="PC479_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805594.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3469..3921
                     /gene="accB"
                     /locus_tag="PC479_003073"
     CDS             3469..3921
                     /gene="accB"
                     /locus_tag="PC479_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2805595.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            3937..5298
                     /gene="accC"
                     /locus_tag="PC479_003074"
     CDS             3937..5298
                     /gene="accC"
                     /locus_tag="PC479_003074"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2805596.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5313..6167
                     /locus_tag="PC479_003075"
     CDS             5313..6167
                     /locus_tag="PC479_003075"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2805597.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6188..7132
                     /locus_tag="PC479_003076"
     CDS             6188..7132
                     /locus_tag="PC479_003076"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2805598.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7321..7581
                     /locus_tag="PC479_003077"
     CDS             7321..7581
                     /locus_tag="PC479_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2805599.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7734..8105
                     /locus_tag="PC479_003078"
     CDS             7734..8105
                     /locus_tag="PC479_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2805600.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8135..8980
                     /locus_tag="PC479_003079"
     CDS             8135..8980
                     /locus_tag="PC479_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805601.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT         3522 a          945 c         1529 g         3065 t
ORIGIN      
        1 ttagtaaaat atgcaaaaac taattgatta tgtaccttgt atgggtatat aatagatatt
       61 gttttaataa aatatttgaa agggaatgat ttgatgaaaa ttactttacc agaaacagct
      121 attgatactt taaaaagtat cttaaaagac aatcaagata aacctaataa tataagagta
      181 tactttgcag gtgttggttg tggaggtcct tcttttggat tagctttaga tgagaaaaaa
      241 gaagatgact taacttatga agttggagaa ttacaatttg taatgagctc tgatgaatat
      301 agtcagtatg gagatataat aatagaagat acaggatttg gatttagagt tattcctgaa
      361 aatatgaaag atcaaggtgg aggaggatgc tctggttgtt cagggtgcca ctaagattaa
      421 gtttttagta agtttagaac tctctttata gaaataaatt tctattttaa gagagttctt
      481 tttatttttt ggtgagataa tgtataataa aaatgtattt tactaatttt ttatatgtaa
      541 ggagaattat tgtgagaaga agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata
      601 caaaatatgt tttaagagac gatatagaca aattaaaagg caagtggaat ttaaagttta
      661 gaaatgataa tccaatacat gttgaatttg ggactggaaa aggtaagttt ataacaactt
      721 tagctaagca gaaccctgac ataaattata ttgccatgga acttaaggaa gaagttcttt
      781 taaaagctgt tgaaaaagca gatgcttcta atttgaataa tatactattt ttatggggag
      841 atgtaagcaa tattttggat tactttgaag ctaaagaatt atcgagaatt tatataaatt
      901 tctgtgaccc atggcctaaa aatagatgga gtaaaagaag actcactcat tctggatttt
      961 tagaaatgta caatagggtt ttggaagatg atggcgaaat tcactttaaa acagataatg
     1021 aaaaattatt cgagtttagt ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata
     1081 tttctcttga tttaggaaat agtgagtatg aaaataatgt tacaacagag tatgaagaca
     1141 aatttatgtc acaaggtatg cgaatattta gatgtgaagc taaaaaacgt aactagtaat
     1201 atgtttataa gtttaatagt agaaatttat tttaatttat attaataaag tgtacgaaag
     1261 taggtatata taacaaggaa acagaactct ctcatagaaa ttaaattatt ataataaaaa
     1321 atttatgtgg gagggtacta tgaatttagc taatttaata ccatttatta tttctgcagc
     1381 agctttaagt ccaataaatt caaatgataa ggttttgagc gaaaatcaac ttaaagatat
     1441 ttttcctgat gcaaatttaa gagcagttgt aaaaagatat ataaatccag atgagatgac
     1501 tataagcaat ataaaggctt tagatggaga attttatgca actggtgaga gtatttcaaa
     1561 tttaaaaggt atatcatatc ttgaaaatgt agacaatttt attttttgga ataataatat
     1621 aaaagaggtt cctaaggaag ctttaagtct aaaagatatg gattcaataa atcttgccaa
     1681 taattatttg attgatgacg atgtagtaaa ttctctttct cataatggtg tggatgtaaa
     1741 ttgcgattta aattttattg atacaaaaga taatcagtat aaactggact ctaaatatca
     1801 taatgttgat atagcaaaag gtgaaagtat tgatttgaga aaaataatta cgaagaaaat
     1861 tgatagttat tataaatact gggaagttac tgataacctt ccaaaagatt taaattttat
     1921 tgtatctgta agtgataaga gtgtactttc atgtgaagat atgataatta aaggtaataa
     1981 aacaggtaaa gcagtggtaa aagttatact aagcgacaaa aatgatttaa atacatctca
     2041 agaagttcta attactgtca atgtaaaata atctgtcaac ttattcaatc attctatttt
     2101 caatttaagt gtataatcaa aataaaggtt gttagtaaca gaacgctaat attagtgatt
     2161 tgttactagc aacctttaaa ttttatagaa atagattatc taaaaaattc catttcttct
     2221 gttagtagcc catattccta ttaaaattat cagtgaatat attatataca aaactattct
     2281 tatatattgg acatctaatc caaaaaattc aatgttactc tgtgaggtat gcaacatcac
     2341 aaggattgca gatataccaa taagcaaagg attgattact atttgcttat cagttcttaa
     2401 aatcattatt ttacccatcc aaattattga taatataaaa aataaaattt taagaccaaa
     2461 ttctaatgta tctaccaaag cgaaaacatc ccttcattta taataaattt ttcagctcaa
     2521 caaaatataa aaccctacaa acaacttttg aatccataat aagataactt gattaagcac
     2581 tatgaaataa gtactataaa acttcatcta cattaatata tactacacta tacttcaaat
     2641 ttcttaaaca taaattaatg aaattaacaa aattttattc agaaacatat aaagatatat
     2701 tttactattt aaatgcatat cattatatat acagttttag gaggtgaggt atatggaatc
     2761 taaatttttt aattggtaca ctcaatcctt agggggaact tttggcatta tatcatgtat
     2821 atgcgcatat ttaaatggat acatgtttgt ttatacaaat agtgctagca aatattttga
     2881 aagtatagga tttttgggaa taatttcaag ttatacacta cttccacttt gtatgcttac
     2941 tttgattttt ggagttatat attcttatac tccagaaaca tccgacaaaa gtatttttaa
     3001 tatacctttt tttactttaa ataaaagatt aatacttatt acagtagttt ttggttttat
     3061 aggtgctaga gtttacttta taattcctgc aattctaata acttttaacc tatatagcaa
     3121 tgttttgata gctaaattaa agtacatcct atgtaaaaat aaagaaaatg atactaattt
     3181 gaaagaaagc gaagtctaaa ttgcataata ttggtatttg tcttaaaaat tcccgaggta
     3241 ttaatttgta ttctcttgta ttttgcaaaa aatattgaat tttattcatt ttatacttat
     3301 aatttatatt aataatggaa aattaaaaca aatataagtt actggttgtt aatttaaaat
     3361 taaaagatat attgatgtgt tgattctcta gtcagcactt tttcttttat aaatttgttt
     3421 tttgtgatat aatattagca ccaggtaata atacaagggg gtaacaaaat gaacatcaat
     3481 gaaattaagg aattattaaa ggccatagac tcaactaact tagaatatgt aaaacttgaa
     3541 agtagcgatt taagattaga agtatctaaa aaagctcaat ctacaagttc acctgtttta
     3601 agtgtacaac aagaatcagt agtggatctt tctttagaaa agccagttgt aaacgataca
     3661 cctgtcacat ctaatgagaa tctatcagta gtagtagctc ctttgatggg aacattctat
     3721 gattctccaa gtccagatgc agatagcttt gttaaagttg gcgatgtagt tgaagaaggc
     3781 gacactttat gtatattaga agcaatgaag cttatgaatg agataacaag tgaaattaaa
     3841 ggtgaaataa ttgaagtatt agttagtaat gaagaactag tagaatataa tcaaccttta
     3901 tttaagataa aaccattgta gtaggaggta tttaagttga taaaaaaaat actagttgca
     3961 aatagaggcg atatagcagt tagaatcatc agaacatgta aagaactagg tataaaaact
     4021 gttgctatct attctgaaat agataaagat tgttttcata gatatatagc tgatgaatct
     4081 atttgtatag gaccaaataa tataagtaag agttataata atatagaaaa tataatctat
     4141 cttgctctta aattaaagtg tgatgctatt catccaggct ttggattttt atcagagaat
     4201 cctgaatttg caaagcaatg tgaagataat aatattattt ttataggtcc aacaagagaa
     4261 caaatgattt tgatgggaga taaatctaga gcaagagaaa ctatgatgga attgaatatc
     4321 cctgttgttc ctggttcaga atctgttttg aaaactaagg aagaagcttt agaagttgca
     4381 agagaaattg gatatcctgt tatgataaaa gcctcaagtg gaggcggtgg aaaaggtatg
     4441 agaatcgtta gaaaagaaga agaacttttt tcaaattttg atatggcaag ttcagaagct
     4501 ctagcagcat tttctaatag tgatttatat atggaaaagt ttatagaaaa tcctagacat
     4561 atagaagtac aagtatttgg agataaacat tcaaatgcaa tacatttagg tgatagagat
     4621 tgttctatgc aaaggcgaaa tcaaaaagta atagaagaat ctttaagtcc atacctttca
     4681 gatgaagaaa gacaaaaact tcataaaatt gcagtagata tagtaaaagg agtaggttat
     4741 atcggtgcag gtactattga gtttatagtt gacaaagata aaaactttta ttttatagag
     4801 atgaatacaa gaatacaagt tgaacatcca gttacagaga tggtaacaaa tcttgacctt
     4861 ataaaacttc aaatatctat agctaatggg gataaaattc cttttaaaca agaagatatt
     4921 acttttagag gtcatgctat tgaatgtaga ataaatgcag aagattcatc aaagaatttt
     4981 gctccatcac caggtaagat agaatcatta aatttacctg gaggttttgg tgttagattt
     5041 gatacttttg tatatgcagg atatactata ccacctttat atgattctat gataggtaag
     5101 ttaatatgtt gggctgaaac tagagaagaa tgtataagta gaatatatag agctctagac
     5161 gaaattattg tagaaggtat taatacaaat gtagaatttc aaaaggcact tgtaacaagt
     5221 gaggagttta gagaagatac tcatcatacc aaatttatag aagatgtctt tatgaagaaa
     5281 gaatttgcta cattataagg agtaaataga ttatgataaa aaaattttta tcatcaaaag
     5341 aatctaagta tgttacgatt tctttagatg acaactttaa aaagaatagt gtagatgata
     5401 aattttggac ctattgcaaa ggttgtgata gtcatgtatt tagaaaggac atagaagaaa
     5461 attcatttgt atgtccaaaa tgttcaagac attatggctt aagagcaaga aaaagaataa
     5521 atttattgat tgataaagga acttttatgg agtttaattc tgatgtagaa tttcaaaatc
     5581 cactaaattt tcctaaatac aaagaaaaag tagactctta taaggaaaaa acaaaagagt
     5641 cagaagctgt tgtcacaggt tatggaagaa taaatggaat taaaacagtt atttgtgtaa
     5701 tgaatcctga ttttatgatg ggaagtatgg gttctattgt tggggaaaaa ataacttatt
     5761 caatagagta tgctgctgaa aataatttac ctataattat ttgttctgct tcaggaggag
     5821 ctagaatgca agaaggtatg gtatctctta tgcagatggc aaagacttct caagcacttt
     5881 ctaagcttga agaaaaatca ttgccatata tatcagttct aactgaccct actactggag
     5941 gagtaactgc tagttttgct atgcttggtg atattataat ttctgagcca aatacgttga
     6001 taggttttgc aggtccaaga gtaatagagc aaacaataaa tcaaaaactt cctgaaggat
     6061 ttcaaacttc tgagttttta cttgaaaaag gttttattga catgatagta gatagaagaa
     6121 aaatgaagga agtattatat caaattcttg ctatgcacaa gaaatagggc ataaaggagg
     6181 aactgttttg attgaaaata atagtgaaaa aataaaagtc ttagaaaatg atattaaaca
     6241 attaataact atttctaagg caaataatat agatttaagt gataaaataa attcacttaa
     6301 tgagaaattg gaaaagctta aggaagatgc tttttcacat ctatcagcat atgaaaaggt
     6361 aacattaagt agagatataa agagacctac gacattggaa tacatagaac atatttgttc
     6421 taatttttta gaacttcatg gagataggct ttttaaagat gacccttcta tggttggagg
     6481 aattggtcag ataggaaagt ttaatgttac tatagttgga caccaaaaag gaagagatac
     6541 aaaagaaaat ataaaaagaa attttggaat gcctcatcca gaaggataca gaaaagcatt
     6601 aagacttatg aaacaagctg aaaaatttaa tagacctata ataacgttta ttgatacatc
     6661 aggagctttt tgtggtctag aagcagaaga aagaggacaa ggagaagcta ttgcaagaaa
     6721 cttacttgaa atgagtaaac tttctgttcc agtaatcact tttgtaattg gtgaaggtgg
     6781 tagtggagga gctttaggta tcggagtagg aaatgatgta tgtatgttag aacattcagt
     6841 ttattctgtg atttctccag aagggctttc tagtattcta tttaaagatt catctaaagc
     6901 taaagaagct tgtgatgtta tgaagcttac aagtaatgat ttatatgatt taaaaataat
     6961 agataagatt ataaaagaac ctcttggagg agcacaaaaa gatgtagaag ctgtttcaaa
     7021 agaaataaaa gcttacattt tagaaagatt aaatcattac aaagatatgg ataaagagga
     7081 aattatagct caaagatata aaaaatttag aaatattgga aaatgcttat agaatataaa
     7141 gaaatttgta gttttttcat aataaaatgg ttaaaaaaaa taaaggtttt cttaaaacga
     7201 ttatagaagt atatttttat aaaagtatac tttaaatgat gattgtaatt agtaaatggc
     7261 aattatgatt tagtacttta ggtaatggat actttaacaa attaacaagg agatgttgaa
     7321 atggaggtac taaaagtttc atcaaaatct aatcctaatt ctgtagctgg agccttggct
     7381 ggagtactaa gagaacgagg cgttgcagaa atacaagcta taggagctgg agctctaaat
     7441 caagcgataa aatcaattgc aatagcaaga ggttttgttg cacctagtgg aatggattta
     7501 gtttgcattc ccgcatttac tgatatagaa atcgaaggtg ataagaaaac agccattaaa
     7561 cttataattg aacctagata atatatctct ttattagaat ttaattaagt tttaaaggtt
     7621 ggctatagaa ataggacact atagtgaacc ttttttactt tatatgtttt attttatgta
     7681 acttttaatg tataatctta agtaaacaaa taagattgga ggaaatatac attatgaaat
     7741 taaaattatt ggtagaagag tatgcagtat gtagattaaa taatgacagt aaaataccaa
     7801 catggataga tacagagaag ttttactcta taactagaac tgatgatgaa ttaagtgttg
     7861 tatgtttaaa taataatatt ccttctgatg ttaagtcaga aaaggagtgg agaatactta
     7921 agatactagg tcctttagac ttttcacttg taggaatttt atctaagtta agtagtttgt
     7981 tagcagacaa caaaataagc atatttgcaa tttctactta tgatacagac tatatcttag
     8041 taaaagaaaa ggatatagaa aatgcttgta aaattttaag ctgtaatgga tatgaagtag
     8101 agtagttaaa tacttttaca tgaggattta acttatgatt tatataatcg aaattttaaa
     8161 aataagtgta ataaatattt tttgtttcat aggaatattt attgtatttg gattgatttt
     8221 tagcattaca gaaaatctaa ataataaatt tatattttca tcttttggaa aaactggaat
     8281 tattgtaact ggagctatag gaacatttgt tcatgaattg agtcatttga taatgtgttt
     8341 aattttcatg cataaaataa attcagttaa gtttttcaga ccaatagaaa gtaaaaatga
     8401 tggtatacta ggatatgtaa gtcatagcta taaaaaagat aatctttatc aaagtatagg
     8461 aaatttcttt ataggaattg cacctattat aggtggaaca ataatgatta ttttaatttt
     8521 taagatatta ctgccagata gttatatgaa agtgactcaa aatatagatt taaagttata
     8581 tgtaagcatg attaataatt taaatataaa tggttttata cacattattt taggtgatat
     8641 ttccaatttt ataagaataa tgttgttaac acctagtgta tattcattga agtattttat
     8701 atttatgttt ttaatgtatt ctatatctac acatatgtca cttagtagtg ctgatttaaa
     8761 aaatagctta aatggcctga gctttatagt tatagttatt tttgtaataa gttcaattac
     8821 atacttattt ggatttgata acttgaatat atccagttta atagttaagt ataacatatt
     8881 tataagtttc tttatggcaa tagggtttat tttttcaata gtaactctca taatatccta
     8941 tattttaagc ttagtcagcc catttaaaaa ctttagttaa ataaatacat attttactaa
     9001 tatttggttt cgttagaaac atataaaaat attatataaa aaagattcta gtagagcata
     9061 a
//
LOCUS       DAMJYX010000121         8721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJYX010000121 DAMJYX010000000
VERSION     DAMJYX010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.126"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            128..1909
                     /locus_tag="PC479_003080"
     CDS             128..1909
                     /locus_tag="PC479_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2805602.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(1991..2521)
                     /gene="lepB"
                     /locus_tag="PC479_003081"
     CDS             complement(1991..2521)
                     /gene="lepB"
                     /locus_tag="PC479_003081"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2805603.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(2561..3385)
                     /locus_tag="PC479_003082"
     CDS             complement(2561..3385)
                     /locus_tag="PC479_003082"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2805604.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3642..4550
                     /locus_tag="PC479_003083"
     CDS             3642..4550
                     /locus_tag="PC479_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2805605.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            4694..6139
                     /locus_tag="PC479_003084"
     CDS             4694..6139
                     /locus_tag="PC479_003084"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2805606.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            6450..6902
                     /locus_tag="PC479_003085"
     CDS             6450..6902
                     /locus_tag="PC479_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2805607.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(7237..8433)
                     /locus_tag="PC479_003086"
     CDS             complement(7237..8433)
                     /locus_tag="PC479_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2805608.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT         3447 a         1111 c         1268 g         2895 t
ORIGIN      
        1 ttttttagca gttttgtatt tatttgtatt tattttgaat ttatttacta aatttgccta
       61 taaaaagtat atataaaaat atagttcaaa atggtataat taagtataac agaaggaggt
      121 aatgtaagtg agccaataca taaatataaa ttctcagtta gacgccctaa aattgaagga
      181 gttcgagaaa atattttacg aaaatatcaa tgaggacgtt aaagtagtgc caaaagttac
      241 tccttttaaa ggaataaata ccgatttgtt gtatgttaaa gatggaagaa ttttatttat
      301 aaaatttatg gatacaacag aagatatatt ttttatttta gaagaagagt tacttgaagt
      361 tatgaatgag gaatatgaac ttttgaaact aaaaatggga caaaagaata gaaatataag
      421 ttataactac gtttacatta tgccatacgt tgaagttgag gaaacatatg aatttgaaga
      481 atttgttaat aataatataa tagataaaaa taagcttcaa gatataatga ataagggttc
      541 gctggaggaa tatcttaatg atgagaatga tgaaataaac ttaaatttat ttttactaga
      601 catatgttca gaatactata taataaatga taaacttcat ctaaatgaaa aatttaaaaa
      661 aatttcattc tacaatgatg actataagta tacagcaact atgatggaag acgttcaaat
      721 taaagatgtt gtttctatta aatatgggaa tacacttatt gaaggtggtt ctggtatagg
      781 caaaacagca attatgcttt ctagggctat aaaattagca aaagtatatc cacatcataa
      841 attagtcata tttacacata caaaacaact gtgcaatgag cttcgagaga ggatagagtt
      901 attatacaaa gataataaca atttagaagt tcacacattt agttctttta tatttaaact
      961 agctaaaaaa ttcaatttaa ttgtagatta taacatgtta aaaaatgatt acgaaaagac
     1021 ctttaataat cttgtaaaac aagcacagaa cattataaaa aataaaaata tgttcaaggc
     1081 tatttttatt gatgaagcag aaagcttttt agagtatgaa atagatttta taagggagtt
     1141 tttatataaa acaaaattca tatttaatgt gtgttcttgt aactccttga atatctctaa
     1201 tagattgaat atatttaaaa aattatataa tggtatagaa tttgatgata agattatctt
     1261 aagtaaaaat tatagacaag ctaaagagat tgtagatttt acaaataaat tttcaaataa
     1321 ttctaactca tatatcaacg aattaagacc aaatacagaa tttagtactt tcttttatac
     1381 taaagcactt agaggtggaa ataagtctgt tgatataata aaagtaagtg accttgatga
     1441 ccaaataagt tctgtaatct gggaaattga gtatttaata agtaaaaaag gtcttgatta
     1501 ttctgaagtt gctatcgttt atccttacaa taagaagaaa ttaaaaagtg gaaaaaccat
     1561 ttactttcaa tatatgttaa aaaaagcatt ggaagaggca aaaattcctt atatatgtgc
     1621 agaagataat ttgacaaata taagtaagaa ggtaggaact acaatagcaa atatatatgc
     1681 aataaagaat ttagagtata aagcggtaat agtatgtgaa ttagagatgt tatacaatca
     1741 aactataaat gatattgaac aagattatca agtaaatgac tttgtaggag atttaaataa
     1801 ggtttattta gctatgagta gagcaacaga ctacctaagt atagtaacaa cttttaatga
     1861 agaagcaagt gatataataa gacttattac agaatcaaaa gatatttagt aaaaaaatat
     1921 ttgtaattta agaaaataaa aagagactga gatttgtaaa ataaacttta ccaattcagt
     1981 ctcttatttt ttagtctact ttcccaatat tatcaagagg taatagtctc accataacct
     2041 taccaagaac ttcatcttca tcaaccatac ctacatcagg gaatctacta tcattactat
     2101 tttctctatt atctcccatt gcaaatagtt taccttttgg aacaacagta tctatatctc
     2161 cagaagtata gttattgtgt atatatggtt catttaataa ttttccattt acatacactt
     2221 tagaatttga tatttttatt ctgtcacctt cagtagctat aactcttttt attaagtctt
     2281 ttttctttcc tgtttctcca tcaactaaat tggttttaaa aactacaata tcgccttttt
     2341 caggtttacc aaccttgtat gatatcctat ttaaaatcag ataatcttta tcatctaaag
     2401 taggatacat tgattctcca cttacaatag aaggtcttat aaattgtaga attacaaatg
     2461 caagtacaat agccatagct attgacttaa tccaatcaaa tatttctttt ttaacactca
     2521 taacttttct ccttatcatt tgttattaaa ttagaataca ttataataga aagaaataag
     2581 caagaaccac tatacccaag actattctat aataaccaaa tactttgaag tcatgttttt
     2641 tgatatagtc cataaaaaat ttaataacta caattgaaac aacaaatgct actacagaac
     2701 caacacctaa aataagccat tcaaatccag taaatccaaa tccagctttt agaagtttta
     2761 atgcactagc tccaagcatt gtagggatgg ctaagaagaa tgaaaactct gctgctacat
     2821 atctagaagt accaagtaat actgcaccta ttatagtaga accagaccta gatgtacctg
     2881 gtataagagc tagacactgg aataatccta tacaaagagc taatttatat gtaacttgag
     2941 aaaaatcttt atacttaggt tttttatttc tcttttcaag ccctatcatt attataccat
     3001 atacaataag agcaatagca actactgttg aattaaagaa taatctatct atatcatctt
     3061 caaacagtat tccaataact ccagatggta taactgcaat tataacctta agccataaac
     3121 ggactgtttc ttttttctgc ttaatattct ttgcgctatc aaaaggattt aattttctaa
     3181 aaaatataac aagtacagct aatattgaac caaattgaat tacaactaaa aatgtactta
     3241 taaatgtatt tgaaaaattc aattttataa actcatcaac taatatcata tgccctgtac
     3301 tacttactgg tagccattct gttattccct gaactatgcc caaaatgact gccttaaaaa
     3361 tttcaagcaa actaaatgtt tccaaatcaa aatacctcca atacaaattt atagtagcat
     3421 attttattca tagattaata aaaaaatgcc accttttact aacttataat accatagtac
     3481 tgtgtagtgc acaatggaag attgatattt tcataaaacc ttcataatat aacaaaataa
     3541 acttaaaagt tatctattga ataaggggta tatttgaggg ggaaatatat ggtatagtaa
     3601 aagtatagct attttatttt atgaaaagga aaggagttat tatgtatagt acaaaaatac
     3661 tagatgatta tgagtatgaa acttttaatg ttttttctac aaaattaaat ccaaacacga
     3721 aacacgatta tttatcaaag gttatactgt ttaaagaatt tttaaaaggg aaagaattga
     3781 tttatgctac gaaagaagat tgtaaaaatt ttgtagacta tatacagact aaatatgcga
     3841 aatcaacttg tgaaaagata tatagttatt tacatagttt ttataacttc ttaaaaaaag
     3901 aaggatacat agatataaat ccatttagat atgtagaaaa acccacagta acaagaataa
     3961 aaacaaaaga tgatgttcta agtatacaag aaataaataa acttataggt atcctaccaa
     4021 aattaaatat aagagataga gtaataatag tctgtctagt tactactggg tgtttattaa
     4081 atgaattagt tagcttaaag tggaaagacc ttatggtaga tgaaaacgat aactcatatg
     4141 taaggttagg taagggaaga aaagaaagag tagttaaatt acatccatac ttttttaaac
     4201 ttttagaaga ttacaggaat tattctgggc ttccagaggt aataatacct agtgatgatt
     4261 ttatttttac aactcaaaag agtaattcta taacagatag aaatgtcaga cttatagtca
     4321 aaaaagcatt agatttagct ggactttctc aatattcagc aagagatttt agacattcat
     4381 ttgcagcaat aagtttaagg cttggggcag atgaaagtga tgtaaaaaat cagcttggat
     4441 ggagtgataa atactatgca attagatata agtatgtatt aaattttgtt gatagtgaaa
     4501 tagtggatta cttaattgaa aaagataatt tggatataaa taaaaaatag taataaataa
     4561 aaaatactat atataaatgt gaacaaaaaa taaaaaagtc ttgttatagt tcgtaaaatt
     4621 atatataatt atttatgtac taatgtgtac acagtaatca cacgacaaaa gatttaagga
     4681 aggtgctaat ataatgatag ataataaata tactacttac tcaaacccac ttacagacag
     4741 atattgtagc aaggacatga gttatatatt ttctccacaa tttaaattct ctacatggag
     4801 aaggttatgg gttgcacttg cagaaggtga acatgagtta ggaatcaata taactgaaga
     4861 acaattagat gagctaagag ctaatataga taatataaat tttgatgaag ctagaaaaat
     4921 agaaaaagaa gtaagacatg atgtaatgtc tcacgtaaaa gcttatggtt tacaatgtga
     4981 aaaagccaaa ggaataatac atttaggagc aacatctgca tatgttggag ataatacaga
     5041 tgttatacaa atgaatgaag ctttaaaact tctaagagta aagcttgtaa atttaataaa
     5101 caatttaaaa gaatttgcac ttaagtacaa agatatgcca gctcttggat ttacacactt
     5161 ccaagctgct caacttacaa cagtaggaaa aagagcttgt ttatgggctc aagatttaat
     5221 tcttgatttg gaagatttaa actatagaat agacaacatg aagttaagag gagttaaagg
     5281 tactactggt actcaagcaa gttttttaag cttatttgaa ggagaccacg aaaaagtaaa
     5341 agagttagat aagaaaattt gtaaaaagat gggataccat tcttcctatg cagtaagtgg
     5401 acagacatat actagaaagt tagattatca agtaataagc atactatcag ggatagctca
     5461 aagtatgcat aagatgacta atgatataag acttttacaa agtttaaaag aattagaaga
     5521 gccatttgaa aaaaatcaaa taggttcatc agcaatggct tataagagga atccaatgag
     5581 aagtgagaga atagcatctt tatctaagta tataatctct gaatctataa gccctgcaat
     5641 ggtacaagcc actcaatggt tagagagaac attagatgac tcagccaata agagattggc
     5701 aatacctcaa gcatttatgg cagcagatgc tatacttgaa atagggataa atgtaactga
     5761 tggtttagta gtatatgaaa atatgataaa tagacgtgta aatgaagaac ttccttttat
     5821 ggctacagaa acaatactta tggaagctgt aaaaagaggt ggagatagac aggaattgca
     5881 tgaaataata agagaatatt ctatgaaagc tgcctataga gtaaagcatg aaggcaagga
     5941 taataactta attgaactta taatgaatga tgattcattt aaaatgagca aggaagaaat
     6001 actatctata atggacccaa aaaactttat aggtagagct ccagaacaag ttgtagaatt
     6061 tgtaaatgaa gttgtagaac ctgctataaa agattataag gaagacttag gaaaagtaga
     6121 tgtagaccta agagtataga attgtacaga taaaatataa ttaaaaacgg ctatcttatt
     6181 tataaataag atagccattt taatgggcta gcgtcttctc tccaccgctg tgaagagttc
     6241 ttctttactt actataccat gaatttaata aaatagctat ataaataata tttaatagga
     6301 ataatataaa taaaaataaa aaaatgataa gattactaat taaatttaaa cattcagcaa
     6361 aaactattgt acttgcaata tttttaatgt aaactgaaat aaagttattt agataattta
     6421 ataaatttta aaacaaccga ggtgattata tgaactatat agtaaaagat accataagtt
     6481 ttttagtaaa aacatttcct aagatttatt ctagcctgta cttagaagac ctaaaaaaat
     6541 ttgcaccaga ttataatgta aataaaacac aattaagagc attagttttc ataaaaaatt
     6601 atggagtaat aagtatgact gatttatgct ctaagttaaa tatagaaaaa ggcagtttaa
     6661 ctagtatggt tgatgattta actgataaga agtatgtaac aaggaaaaga gatttagttg
     6721 atagaagaaa atatctaatt gacattactg aagagggaga taagatagca actgatttta
     6781 tggataaatt aagtgatggt ttaggagaaa aactttctaa attaactgaa gaagacagaa
     6841 aaaaatattt agaagcaata aatactcttc aatatatatt aaacaaagaa gaatttagat
     6901 agaaaaaata taaaaatttt tgtatttaat ggtactaaga ttaattcttt gttattcatt
     6961 taactatata attataagtc taaatacaat ctaaaaagta catcttaaat tttcttgatg
     7021 tactttttta taatttctct aactaaggca agacaagttt tagatgcttt aattaaagtg
     7081 aataaagatt actggccaac actatataaa gaaaatgaaa aatagtcagg tctaagtctt
     7141 aattaatata ttaattaaga aatgtcccct tttcaaataa tatctgaaaa ggggacattt
     7201 tatatcatta aatttatata aattaagttt aaaaaattat ttttgtaaag ctacatattg
     7261 ctctaaacct tcttttaata atgtagcagc tctatgaagg tctttttcat ttaagatgta
     7321 agctaatctt atttcatcac gtcctaaacc tggagtagca tagaatcctt cagcagggca
     7381 aaccataact gtttcattat ctttattaaa ttcttttaac atccaaattg tgaaatcttc
     7441 tgcattttca actggtaatt tagcaactat atagaaagca ccagttggtt ttttacatat
     7501 aacaccttca accttcatta attcattgta taatacatct cttctttttt tgtactcttc
     7561 attaacttct ttgaagtaag aaacaggagt tttgtataac tcaacagctc ctatttgctc
     7621 taatgtagga acacaaagtc ttccttgaca taatttaagt atttgagcga taagaccttt
     7681 atttttgcaa gcgatagaac caattctagc accacaagca ctgtatctct tagaaacact
     7741 gtctattatt ataactctat cctgtacacc ctctaagtta ccacagcttg tatactcaag
     7801 cccatcataa acaaattctc tataaacttc gtctgctatt atccataaat ctttttcttt
     7861 agctatttca gctaggatat taagttcttc ttttgtataa attgcaccag ttggatttcc
     7921 tgggttagaa agaattatag ccttagtttt atcatctact ttagacaata tttcttcttt
     7981 agatggaagg tggaaaccgt tttctgcctt tgttgtaaca gcattaactt ctacgtttac
     8041 agattgacca aatccattgt agtttgtata gaatggttct ggaactaata gattatctcc
     8101 tgggtcacaa actgccatca tagtgaaaag taaagcttca ctaccaccgt tagttactaa
     8161 taattcatct ttttcaaaat tcatatcgta agttttatag taattttgaa gtgcttctaa
     8221 taattctgga ataccttctg atgtagcata ttctaagact tcgctatcaa agtttttaac
     8281 agcatcaaaa aatcctttag gagtttttat gtctggttgt cctatgttta agtgatatac
     8341 tttgattcct ttatcttttg ctgcttgtgc aaatggaaca agttttctga ttggagaggc
     8401 ttgcattgca ctaactctgt ttgaataatt cattttaata ccccctattt aatttaaata
     8461 cgtatattgt attatgtaaa ttgtatgttt gaatacaaga catctttaca agataataat
     8521 aacatactta tgggtaaaaa tcatcaaaaa aaattaatat agctttaaaa taaactttta
     8581 acaaattata gtaaaatacc catgtttatg ccactaaatt atgtattcat aaattaattt
     8641 atagtaagat aatattctga tgtactgata gtgatttttt agttataatc tttcagctat
     8701 agacaaaata tataataata a
//
LOCUS       DAMJYX010000122         8389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJYX010000122 DAMJYX010000000
VERSION     DAMJYX010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.127"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            175..1161
                     /locus_tag="PC479_003087"
     CDS             175..1161
                     /locus_tag="PC479_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2805609.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1330..1478
                     /locus_tag="PC479_003088"
                     /pseudo
     CDS             1330..1478
                     /locus_tag="PC479_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1497..2978
                     /locus_tag="PC479_003089"
     CDS             1497..2978
                     /locus_tag="PC479_003089"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2805610.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3004..3975
                     /locus_tag="PC479_003090"
     CDS             3004..3975
                     /locus_tag="PC479_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2805611.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4231..4833)
                     /locus_tag="PC479_003091"
     CDS             complement(4231..4833)
                     /locus_tag="PC479_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2805612.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5114..6325
                     /locus_tag="PC479_003092"
     CDS             5114..6325
                     /locus_tag="PC479_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2805613.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6393..6590
                     /locus_tag="PC479_003093"
     CDS             6393..6590
                     /locus_tag="PC479_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2805614.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            6624..7595
                     /locus_tag="PC479_003094"
     CDS             6624..7595
                     /locus_tag="PC479_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2805615.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7782..8159
                     /locus_tag="PC479_003095"
     CDS             7782..8159
                     /locus_tag="PC479_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2805616.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3411 a          877 c         1351 g         2750 t
ORIGIN      
        1 aacaacaaaa aatgaaaatt acaattaatt taatgttaaa gactcaatta tttggatgtg
       61 gaatggatag tacaagtagt atataaaaag tattgactgg acaaataact atattttatt
      121 ttaaatatat aatataatgt aataaaatat atcaaatgta gaaggtgata acctatgaaa
      181 aaaataacaa taaatgatat tgctaacttg gcaggagtat ctaaaagtac tgtatcaaga
      241 tatctaaata ataaagatat cagtgattct actaaagaga aaataaagac aataatagat
      301 gaatatggat atgagccgaa tgcatttgct caaagtttga gagctaaaaa aacatacttt
      361 attggtatta taacaccgtg tttagattct tttgtaaaat ctaaaattat gatggcaata
      421 gatgaagaac tcaaagaatt aaaatataca tcactaatta ttaatactaa tagaaaaata
      481 aggtcagaaa tagatagtat atcaaaatta gctagtctaa aagttgatgg aataatacta
      541 attggtaccg aaataactaa agaacataag aatgaaattg aaaagttaga tataccgata
      601 gttgtagttg gacaaaaagt tgatggtatc aatagcatag tgaatgatga ttatggagca
      661 ggatataaaa tgggtcaata tattgctaat aaaggttaca aaaacattgt atatttaggt
      721 gttgatgaaa gtgatatatc agttggttta aatagaaaaa atggagtact taatgggtta
      781 aaagataaag ggtatgatgc aaaagtattt tatactgact ttgaccaaga aacgtctata
      841 caaagaagtg gagaaatgct agaaagtgaa aatccagata taataatatg tgcaactgat
      901 aatatagcaa tagcaacaat gaaagaaatc aataagagag gtaaaaatat accacaagat
      961 atatctgtag ctggatttgg gggatatgat gtattgtcta ttataagccc taaacttaca
     1021 acaataaagt ttgaaaataa aaatgcaggt aaagtagcag caaatacgat agtaaattta
     1081 atacaagaaa gaaaagaacc actattaaag gagattaaat ttgaattaat agaaggtgaa
     1141 agtacaataa ataaaaatta agatacacat tacatatatt agtaatgtgt atttttttac
     1201 taaaaaaact ataattctaa aaattaggaa aacttttttg aaagaaatat tgcatctgaa
     1261 ataaatttat gctaatcttt aattaaaccg gtttcgaaac cggtttaatt aaaccgatag
     1321 aatagcaaaa tgagtgcaaa aataactgat tgtaacaaag tatcaaagta attattagag
     1381 tacataggaa taaacaaaat ataaaagatg tagcacattg tgtaataaga ctaagaattg
     1441 ttttagatag caataataaa gcaaatataa aattataaaa gaaaaggaat aaaaatatgt
     1501 acaaagagcc taaatataga accatattag aatcgacaaa agaagaatta aataaattaa
     1561 gaaatatatc tttaaatgat aaatataaac cactatttca tatacatcca caacatggac
     1621 tacttaatga cccaaatgga ttagcatatt ataatggtaa gtatcatgta ttttatcaat
     1681 ggtatccata tgatgcaact catggaatga agcattgggc ttatgtaagt tctgatgatt
     1741 ttgtaaactg gaatagagaa gatgtagcat taataccaat cgaaagttat gaatctcatg
     1801 gagcatattc aggtaatgcg atagaggtag atggaaaatt gcatatgtat tatacaggga
     1861 atataaaata tagtgcagaa gatagatatg catatcaaaa cttagctata atgaacaagg
     1921 atggtaaaat aactaaatat gaaaataatc caatagtaag tgaaatacca aaaggatata
     1981 caggacatgt aagagaccca aaagtattta aaagaaagga taaatacttt atgttacttg
     2041 gagcacaaac aagtgataaa aaaggagcta taatagtata tgaatctaaa aactctatag
     2101 attggaattt taaaggagaa ttaaatgtaa aaaatataga tgaagatttt ggttacatgt
     2161 gggaatgtcc agattatatt aatatagatg aaaaagatat attaatattt tcaccacaag
     2221 gagtagaacc taaaggattt gattatcaaa atatatataa tgtagtatat gctattggta
     2281 atatggattt agataattta acatttgaaa tagatacaat gaaagaactg gaaaaaggat
     2341 ttgatttcta tgctccacaa acatttataa aggactcaca aataatacta tttgcatggg
     2401 ctggaatggg agaagtttta taccctacag ataaaaacaa atgggcacat tgtctaactg
     2461 ttccaagaaa attaaatata aaaaataata agctattgca aatgccagta gatgaattaa
     2521 ttaaattaag atatgatgaa acaagtgggc aaaatactat aaagaataat attaatatta
     2581 tagaaaatga tgaaaatgta tatgagctta atattaatat aaaaaatatt gacagcaata
     2641 aatttgggct ggaattattt tcatcacaag atgaaggtgt aaaattagaa tttaacaaat
     2701 taggaaacat tgtaacttta gacagaagca actttaaaaa agttttcggt gttgaatatg
     2761 gaacaaatag aaaagaatat ataaatattg atgaaaatac taatatcaaa gtactagcgg
     2821 atagaagtat tttagaaatt tttataaatg aaggagaagt agtatttact agtagaatat
     2881 ttgctaaaga aaattctaat caaataaggg tttatagtga taaaatagta gagtatgagt
     2941 atactaaatt taaattaaag caaggtattg aattataaaa acacattata aggggtagaa
     3001 aagatgaaaa aagtaattag cataggagaa gctttaatag actttatacc gaatcaaaat
     3061 ggtggtaaat taaaaaatgt atcagaattt agaagagtag caggaggagc tccagcaaat
     3121 gttagtgctg ttgtagcaaa gcttggagga aaatcgagtt ttatatctaa gttaggaaaa
     3181 gatgcatttg gtgattatat aatagatgta ttaaatgaag taaatgtaaa tactgattat
     3241 gtattgagaa caagtaaagc aaatacagga cttgcttttg tatcattaaa agaagatggt
     3301 aatcgtgatt tctcatttta tagaaatcca agtgcagata tgcttttaga agctgatgaa
     3361 gttaaaaaag agtggtttaa taattgtcat atcctacatt tttgttcagt agatttaata
     3421 gatagtccaa tgaaattagc acataaaaaa gcaatagaat acgcacttga aagtaatagt
     3481 ataataagct ttgaccctaa tataagatta cctttatggg atagtgagca atcttgtaaa
     3541 aaggcaatat cagaattttt accttttgct catattgtta agatatctga tgaagaatta
     3601 gaatttataa caggagaaaa tgatatagaa aaatcattac ataaactctt tgtggggaat
     3661 gttgaactag tattatatac aaaaggaaaa gatggagtag atgcatacac taaaaaagta
     3721 aaaggtatgt gtaaaggggt taaagtaaat gcaatagata caaccggagc aggagattct
     3781 tatataggtt catttttata caccttattg tataaacaaa taacattgaa tgatataaaa
     3841 gaaatgaaac aagaaacgtt aaatgagtat ttagaatttt ctaattatta tgcagctcgt
     3901 agtacaacta ttaaaggtgc gataagttca tatgcaacaa aggaagaaat aactgaattt
     3961 ataaaaaatt tatagtagga atgagtaata ggttttgaag gaagtaacat gtaaagagtt
     4021 tttaaggatg tttactatga aaataattaa catataaaaa gcaataaaga ggctgtctca
     4081 aaatagagat aagcctcttt atcattgttg aaatgataaa aaatatttta attattgtta
     4141 ctttttagaa gaatatatat cttattttaa ataagcaaat tatgtaagat ttatttatat
     4201 tatcaatact ttacttggag tattatgtct ttagcagtta tctacttcaa ttgaattatc
     4261 taaaatataa taaatttgtt ttgcaaattg acgagaatgt tcttcttttt ctttattcat
     4321 aaaattaggt ttacctaaat cacgttcaac aagcattcct atatatttta actgtgaatg
     4381 cttacagtag cgcttcatac cattttccca aatatcagca cccttttcag gtctatatcc
     4441 acatgttgta ataagtgcta ctggttttcc tgcccataga gtggggcctt tctcattacc
     4501 atagtattta ttcattccat acaccaatct atcaagtagt gctttcattg gagaagtgca
     4561 gtaccaagaa taaattggag tagcaaaaac aattaaatca cacataagaa ttttcttgaa
     4621 aattttctga acatcatctt tacaataaca tccaaaatca aataagttat cctgacattt
     4681 tttacaagca atacatggct ctattttaca atcatataac catattactt caatatttga
     4741 attataattt tctaattcct caataaaagg ttttaaaata gcagcagtat tacctttttt
     4801 cttaggactt cccataagaa tgcatacttc catattgata cctccacagt attttaaaat
     4861 agtttttagt gcttaatata gttttaggat actatgagaa tacgttaaca acaatacact
     4921 ttgtatattt gtaataaaat tgaaacaaat tgttttatca ataaaagttg gaatgtatct
     4981 ataattaaaa atattttagg tatgttttta catatatgtg tgtagttaac tatggtaatt
     5041 tattgtaaaa ttatcttata ggattataat tgcttaattt atgattatat ttaaaatctt
     5101 ggaggtaaaa aatatgtact caaatgaaga ctataaattt atagaagaaa atgcaccatg
     5161 ttatatatat gatgagacta atataattga aaaatgtaag gaattgaaaa ataatatttt
     5221 aggttttgat tttttatatt ctataaaaac aaatccatat aacaaaattg taaaaagcat
     5281 tgcagagcaa ggttttggag cagatgcttc ttcaactaat gaagttatga tttcatcaga
     5341 atgtggagtt tctaaagatg atatttatta ttcatcacca ggcaaaacag agattgatat
     5401 agaaactgtt ttagataaat gcataattat tgcagatagt attacagaaa tagaacttat
     5461 aaataagata tctaaaaagt ataatcagaa agttaaaata ggaataagaa taaatcctga
     5521 ttttactatg gatgatgata gtgctaaatc aagcaaattt ggtatagata tagaagatat
     5581 gagcagtgtt tttaagatgt taagtatgtg tgattatgta tctgtagttg ggattcatat
     5641 acatattaaa tctcaggtat tggattttga aaagttagga cattattatg aaaaatgttt
     5701 ttttatagct ttaaatttaa ataaaagtaa gtatattaat attgaattta taaattttgg
     5761 tagtggactt ggagtagctt atgataatct tagagaaaat ccacttaatc ttaaagagct
     5821 ttcaaaatta actcaaaaga ttgcttttac taattcaaac acactaaaat caagacttct
     5881 tattgaatca ggacgtttca tagtttgtaa atcaggtaat tattatgtta aaattgtaga
     5941 tattaaagta tcaagaggga aaaaatatct tattgtaaaa aatggactta atggatttat
     6001 gagacctatt atttctaatt taattgaaaa tattacttgt ggagtagaat tagaaggtca
     6061 agagccatta tatacatcaa atcatgcctt ttctctttca gtattaaata atttaaaaga
     6121 agaagagtat gtaaatgttg ttgggaatct ctgtacatca ttagatgtaa ttgcaaataa
     6181 tgttaaattg aaaaaagcaa acataggaga tttgatttca attagtaatg ctggaagtta
     6241 ctcctattct ttatcaccgc tattattttc aagttatgaa tttcctaaac aatattataa
     6301 aagaaaagat ggatatattg agtaaacaat agcataaatt aataaactta gtattattga
     6361 aaaaatttaa aatgcagggg gtgtaattct atatggaatt gtggaaaaga aatttatttg
     6421 tatgttggat aggtatgttt tttagttcta tagggatgag ccaaatagca cctatacttt
     6481 cattatatat taaacagttg ggggtaactg atgttagttt aatacaacag tattcaggta
     6541 tagtttttgg atgtaccttt gtagtagcag cattcttttc gccaatttga gggaaagctg
     6601 ctgataaata tggaagaaaa tcaatgcttt tacgtgcaag tattgggatg ggaatagtta
     6661 tatttaccat ggcgtttgtt caaaatgtct atcagttatt aggattaaga atacttcaag
     6721 gagtttttac gggttatgct actgcatgta ctactcttat tgcaacacag acagataaaa
     6781 atcattcagg atgggctttg ggtactcttg caactgcatc aactactggc tcattaattg
     6841 gtccaacagt aggaggatat atagaatcaa tcttaggtct taaaagtaca tttattataa
     6901 caggaggatt gttgtttgta tcatttatca tctctatttt atttgtagta gataatttta
     6961 aacctaaaga aataaaagaa tcaatttcta tacctaaaga acaattaaat atattgccaa
     7021 ataaattttt aattgcatct atttttgtaa ccacattcat tactcaacta gctttatact
     7081 ctatagaacc aattgtaacc atatatatat cacaacttac aaattttgca tcaaatgtag
     7141 ctttaatagc tggattgaca ttttctgcat caggattagc aaatttatta gcagctcaaa
     7201 aacttggaaa aatgtcggat aaaataggtc ctcaaaaagt attgttaatt tcacttttat
     7261 gggctgggat tatatttata ccacaagctt ttgtaaaaac tgcatggcaa ctgatgttgt
     7321 tgagattttt attagggttg tcagttgcag ggcttaatcc ttctgttaac agcttgctta
     7381 aaaaaattgc accagaagaa tatgtgggaa aaatatttgg atataatgca tctgcccagt
     7441 atataggctg ttcatcagga gcatttttag ggggacaaat atcagcacat ttaggtatta
     7501 gaacagtatt tttttcaaca agtttattgt tgtttatgaa tgcactttgg atgtataaat
     7561 ttactggatt gtttattaaa gataagacta aataatcata ctatttacat ttctaatttt
     7621 atgatgtaaa ataaatattt tttatgtaat ttatcataga attatataaa aaataaccct
     7681 atggtaaaaa ttggaaaata ttattatcga caaaatcttt gacttaaaaa ctatattgcg
     7741 atagtattaa tttaataaaa gtttgagagg ggcgtaaggt tatgacaatt tcaaaaatac
     7801 cacaagcaga attaaaagtt atgaagttta tttgggaaaa aaatgataca gtaacatcaa
     7861 aagaaactat tgaagctatg gaacgaaaat atggttggaa acaaacaact acattgacac
     7921 ttttatcaag acttgttaaa aaacattttt taagtgcaga aaaaatagat agatacactc
     7981 attatacaat agttgttgga cataaggagt atttaagtgt tgagacaaaa gattttttaa
     8041 gcaatataca cgacaattct ttacagagtt taatttcagc actacatgat gatgaagttg
     8101 ttgataaaga tatgttggat ttatttgaaa gtcattttaa aaatttagaa gaagagtaat
     8161 tatatcagga tagaactcta agaatttaat taatctatgg ctgaaaacca taaatggtat
     8221 tatttaatca gctactcact attcataaag taatattaaa taggttctct aattagtaaa
     8281 atgcctaaaa taattggaat agggagtatc aagttaaatg ataccactta tttaaataag
     8341 aaatgatttt taaaatttaa aataatttta taacttgtca atattcatt
//
LOCUS       DAMJYX010000123         8324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJYX010000123 DAMJYX010000000
VERSION     DAMJYX010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.128"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(132..1001)
                     /locus_tag="PC479_003096"
     CDS             complement(132..1001)
                     /locus_tag="PC479_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805617.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(1098..1955)
                     /locus_tag="PC479_003097"
     CDS             complement(1098..1955)
                     /locus_tag="PC479_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2805618.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            complement(2170..4188)
                     /locus_tag="PC479_003098"
     CDS             complement(2170..4188)
                     /locus_tag="PC479_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2805619.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            complement(4459..5235)
                     /locus_tag="PC479_003099"
     CDS             complement(4459..5235)
                     /locus_tag="PC479_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2805620.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(5292..6263)
                     /gene="asrC"
                     /locus_tag="PC479_003100"
     CDS             complement(5292..6263)
                     /gene="asrC"
                     /locus_tag="PC479_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2805621.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(6276..7067)
                     /gene="asrB"
                     /locus_tag="PC479_003101"
     CDS             complement(6276..7067)
                     /gene="asrB"
                     /locus_tag="PC479_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2805622.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(7068..8075)
                     /gene="asrA"
                     /locus_tag="PC479_003102"
     CDS             complement(7068..8075)
                     /gene="asrA"
                     /locus_tag="PC479_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2805623.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
BASE COUNT         2677 a         1507 c          756 g         3384 t
ORIGIN      
        1 tgaatatacc attttgtttt ttaataaaaa atcatgctac atcaatagta agcaactatt
       61 aatgtagcat gattttttgt atctaaataa tcttagtatt tacctatcag tatagattga
      121 tatatcaaaa actatagtct tctatattct tctcttttta gcttttggtt acttagatag
      181 taatcaattt catcaattag acgttgttta tcatcaggta atacaccttt taaattagca
      241 taatttgatg catgattact tctaaataca attttttcat tagcatctat attctttatc
      301 atctctttaa tttcttgtaa cacagcctta tcctctagta ttgtaaactc tcctgcctta
      361 agtaaatcgt aaagttcagt attaggttct accattaaac taagtacacc cacataatct
      421 ggtgaaattg ctcctagcat ttttcctgta tcaactgcat gctgatgtgt ttttttagtt
      481 cctccaagac ctgctattac agtggctgat aatttaatcc cacttttttt aacttttcta
      541 ccaactttta ttaattgttc actactaaaa cctttcttaa tatcttctaa tacttcattg
      601 cttccacttt caagccctag ataaatcatt ttaacaccaa gttttcttaa ttctttaagt
      661 tcatcatcag attttttctc tatagattta ggagagccgt acatagtaat tctttcacac
      721 tctggaaaaa cttcttttat atataaaata atatctctta atttctccat tggtattatc
      781 aatgcatctc catctgctaa gaatattctt tctacatgct tataatatct tctaaatata
      841 tcaatttcat tttttattac ttctagtggc tttattttaa attttttaga tttgtacata
      901 ctacaaaacg tacatttgtt atgagaacat cctagtgtga cttgtattat aagactataa
      961 gcctcacttg gtggtctgta aagtggcata tcgtattcca tattaaaatc ccccttgtaa
     1021 taaaatattc attctattat attattaata tttactttaa ataataactt ataaaattat
     1081 attttttaaa ttttagttta agatatattc tttaaaaatt taatgatact attggcagta
     1141 atattaagtt gtttttcttg acttataata gcatcattat ctcccttttg ttttccataa
     1201 tctccaaatt gtgcatgatt tcctccttca atctcaacat aagttgtatc atcaggaagt
     1261 ttttgttttg attctacaag acttttaaaa ttgactactc catctttgct cccccatata
     1321 gatataactt tttttcctaa ttctttcaac tcatcaccca ttggataaga agctaaaaat
     1381 acaacgcctt caattaattt attatcacgt gcaaaatttg atgctgcaac tcctcctaat
     1441 gaatgacctc ctattaccca atgttctatg ttgttgtaag aatccataac tttctgtgcc
     1501 ttattagttc caagtatagc tagattaagt ggcatcttta cgattacaac ttcatatcca
     1561 ctttctgcta tttttcttgc aagtggagca taagattcta tttctacttt agctcctgga
     1621 tacaaaatta aacctttaga aacttttctt ccttttggtg taaatgaaat gttttcattt
     1681 acagttactt caactttact attgctgact agagcttctt ttgccagctt ttgaggttca
     1741 tatgttttat ttatccatat gaaaaagcca agcaaaatca atacaaatag tatacctatt
     1801 cccttaaata tcttattctt tatgttaaat tttctattat tatttttatc atctttgttc
     1861 tcaacctcat cataaagtaa aggtctctca agttccatag ttatactaaa tgtatcatat
     1921 ttcttattac gctttacctt tacatcttta ctcataaatc taacccccat aatatttgaa
     1981 tactattata gtttatccta agtaaattgt tttttcaacc aatgaaaaaa atcaatattt
     2041 taactatttt catataaaat tcataaatat atgttttttg ttttttatat attattttct
     2101 tttagtattt tataaaacaa atagccatat actttatttt aaatatatgg ctattctata
     2161 atttaatatc taagctgctt cttttattga aactttagtt atattactta ttctatacat
     2221 ttctttgaca ccatttagag tttgcagtaa gtttagacat attatcttgt cacctagact
     2281 tttagtattt ataatactct ctaaaattac acttcttcct tcatcatact tatctacatt
     2341 gtaatttgaa atataattag tatctctcaa tattttttca acatcttcat cttttatttt
     2401 atttttatta acatttatat atagattata catattactt attagtaatt tctgattttc
     2461 taaaaaatcc tcttgatgtt tatctttata tatagcattt atcaacataa gtctatcttc
     2521 aataagtgat ggtatcaata ataaattttt tatatagtaa acatcagcta cattttctat
     2581 agacttattg acttcatcaa ttatattttt aactattcct ttatacattt ctataacatg
     2641 ttgatagcct gttttagcat catatggtaa tataaactta tttactatta atgcaataat
     2701 tgctcctaat gctacatatg aaatcctgct tatggttaaa acatttgggt ctcccataac
     2761 tactgctgaa ccaagagctg aaacagttac acataccatt aattttctat aatcaactac
     2821 ataagagttt atataccctg ctaaaagaac tattaacgac cttaatgtac tatcttttat
     2881 tattgaaaat aatacaataa atattacagc tcctataaat gtaccttcta ttctttttct
     2941 agacctttgt atacaatttt ctgcgtaagg ttgtgttaat gaaaagacag ttaacattat
     3001 ccatcttcca tctcttaaat ggaaataatc cataataaaa cccgctacag ctgtagctaa
     3061 agcaatcttt actgcataag aaaatcttat agaatttaaa ttaaagtttt tcttatatat
     3121 tgatagtcgt ttaaattcat gaggtatctt tgattttctt tcagctttct tttcgtctct
     3181 tgattttact tttttataat ctactaaaaa ctcatataaa ttttctaaaa cactataaat
     3241 ttcttttaaa tttatatttt tagtatcctg agcaataaca taatctctaa atcctaatat
     3301 ctctttatca gttatggtat ctttattttc taaacacatc ttaatatagt ttatttcttt
     3361 cgttgctaca actagaacat ctgatttact atttatgcta ctttcttcta aaatattttt
     3421 tatatcttca tcttcaaatt ctttactatc ctttttgtat ctttctagga ttaaacttat
     3481 tctttctaat gaaaataaaa tattcattat gtctatacct ctaatactga tataaaagtc
     3541 atcttttcta ttatcaaaaa taaccatttt caaattttta gatatagaat ttattctata
     3601 attacagcta tctatttttc tcatactaga tttaattata gaactatcat cattaatagt
     3661 attttttaaa agactaattt tttctaataa ttcatcacat atagatatca gttctttatc
     3721 tccgacattt ttcatcctat ttctattcat aacaaattga gcaatcatta ttataactgc
     3781 tccaacagct aaagccgaca atctcatagg aaattcactt tttcctacag gtatacttac
     3841 cataaatata tacattagac caaagggtac atatactgct ctttttaagt cataactaaa
     3901 tacataccct attataaata atgctataaa atttaataaa actcctaaat acatattttg
     3961 tactgcaaaa aatgatagta ttcctaagat tatatttatt gctaaatact tcagtaaatt
     4021 tttaattgga gaaatagtta aatctctttc caagagcgtt agcatagctg ttactatacc
     4081 tacacctata agtgtatttt tacttccaaa taaatatata aatatagaaa taaatgctat
     4141 tataaaaata aacaactttg tttttgatat tactagtttc tttgtcatca tatcccccct
     4201 aaaaatttca acaagaccaa ttttatcata tttgttatct tattcaaaat gcgctttttt
     4261 acggtaggac tttaactttt tttatattta gaactatata attcatatat aaacgtattt
     4321 tttatatata taaccgatat attttcgtat gctcccatat tttttttgtt tcaaatattt
     4381 tttcaaaaat ataaaaataa ctctctcaac aatactaaaa atgcatttat caagagagtt
     4441 ataatttata caataaattt aattaagctt ttctttacct aatatataag ttccaagacc
     4501 cataaataaa gctcctccaa caatatttcc aagtgttaca gctactaaat tataaatagc
     4561 tcctccaata gtaactgtat ttattgttgg tgaaaataat gaaacactat aaattgtcat
     4621 atttgctaca ctatgctcaa agcctgatgt tataaaagca aataaacata agaatatcat
     4681 aattattttt gcagtatcac tattagttct aaacgaacac aacactgata cacatactag
     4741 aatattacat aaaatccctc taaagaaaag agctgtaaat ggcatggatg ctttactagc
     4801 tgctgtattt gcaaaaaatt cagctacagg tcctttgtct attagaccag ttcctacaaa
     4861 tataatgcct aaaaccaaag ctccgattaa attacctacc caactgtatg cccatatttt
     4921 agaagtatct tttatactta cacctttatt taacatacca gcagacataa ccatattgtt
     4981 tccagtaaat aactctgttc ccgtcattat aaccaaactt agtgctattg caaatgataa
     5041 ccccattact atcttagtca taggtgaacc tgcatcagtc aaaagtcctc caatagtaaa
     5101 tatcaaaagt atacctatac ctacataaag tcctgcaaac gcagaagata ccaaatattt
     5161 tactttactt gtgtttaata aatttatctt atttatagct gcattagtta atttatccaa
     5221 agtttcttta tgcataattc cctcttcctt ctattaaatt ttaatttatt caaatatatg
     5281 ttttactcaa tttatagata tttaattcca ctccagtata tgttttcgta cattttagtt
     5341 atttctggaa gctctacacc ttcaagtgcc cattttttaa attccataaa tcctgttcta
     5401 tcaattatat atcctatatg ttcttttcct cctggtgcat ctttatctat atacttttct
     5461 acatatctat aagtattaag tattatcttt attatgctgt cctcatctac ccataaaaga
     5521 aaatcttctg ctagtctagg gtttttcttt cctgttctac ccattatagc taatctatag
     5581 tattttttct catctctagt ccatgcattt gttgggcaat ttagcacaca ctctccacag
     5641 cctatacact tatctttatc tcttaccacc ttattatttt ctgtttttaa tgctccaaca
     5701 gatatcttag aacatttttt tacacatgct ccacaattta cacatctatc cttttcatat
     5761 attggtaaag ccataccaat aattccaaag tcattcattc tagcttttat acaatcattt
     5821 ggacaaccag ttaaagccac tttaaaatgt aaatcatttg gaaatatagc tttttctatt
     5881 cttttagcaa attcagttgt attatattga gcctttggac agactttatt tcctatacaa
     5941 gctgctatat ttcttgtacc tgctgctgga taacctgcgt ttttctcact ttgatttata
     6001 ttcatttttt caataacagg ttgtataatt ttatttattt cttccatatc ttccatattt
     6061 attcctaata tttcaaaccc ctgtcttgta gttatgtgca cctgtccatt gccgtactca
     6121 cttgcaattt tagaaactac agctaaactc tcagggtcaa taagacctcc aggtactcta
     6181 actcttaaag aagtctcata ttttgttttt gtaattctat aagcattttt cattactttt
     6241 ttggtattta aatctcttat cacaaacttc accccctaat caataagatt tttagcatat
     6301 gaataatcaa atactggtcc atctaaacat atgtaagtat catccatttt acaatgtcca
     6361 catttaccta ttccacaaca catttttctt tcataagaaa cccaaatgtt cttttcatct
     6421 aaatctcttt ttaaaaattc ttctactgta aatttcatca tcattggagg tccaactaca
     6481 actatagaag tattgtttat atctttcata tccaattctg gtatgtattt tgttactaat
     6541 cctacattac cactatatcc ttcctctgct ccatctacag taactaaaat atcaagtttt
     6601 ttactccatc tctttaaatc atctgcaaat aaaatatctt ttggtgattt aaatcctact
     6661 attaatttaa acttttcgca tttttcttta ttattataaa aatattcaac tattcctctt
     6721 acaggggcta atgcacttcc tcctgccact actacaattt ctcttccttc ataaagggat
     6781 acatcaaatc catttccata aggacctctt ataaaaaaag aatctccttc tttataatta
     6841 aacaactcgc tagttacttt tcctacattt cttattgtaa agtcaatata atcctctcca
     6901 tatcctgata ctgatattgg actttcaccg tatttaggta tagatatctc ataaaacttt
     6961 ccaggcaata caccttttgt attgcagttt actctaaaag tccattcttt atcagtatgt
     7021 ttaactatat ctagtatttt tgcacttact ggtatatatg ggttcattta tacttcctcc
     7081 ttactaatta ctaaatcatt tattttttct atacactttg aaaatgatat gtattgcgga
     7141 caggcatcat cacatcttcc acagccaaca cacatatgat atccgaatct ttttttaaaa
     7201 tcatgtattt tatgcatagt tttaaatctc attctttcgc cttgtttttt tctaaaagaa
     7261 tttccacctg ctatatctgt atatccatct acttgacaag aagcccaaac ccttcttctc
     7321 tcacctacat tttcgttttc cttatagaaa atatcctgca ttgtaaaaca tgaacatgtt
     7381 ggacatacaa aattacatct accacatcct atacatcttg tatcatattc atcccatatt
     7441 gaatttccta tcatttcatt tgaatcaata ttctctggca catctacaga tattaaatta
     7501 tcagttacat aatcaacatt aaattcagtt gcttctccat taaatacttc aaattcacta
     7561 tcctttatat ctaagtacag tgtatctcct tctatattta atccaattga ataattatca
     7621 gttttgttag aatccatgct cacacaaaaa cagtttctaa aactttcctt acaccctaca
     7681 agtgcaaact ttactttatc tctgatattt ttataaaaat aatctttttc ttcaccatta
     7741 tttagatata tttcatcaag tctttttata ccatttatgt cacaagctct taagaaaatt
     7801 aaaattttct tgttgtctaa atcgctttcc ttaaactctt tttcagtaaa ataaaacaat
     7861 acttgattta tgggaagtac aacttctttt ggtgaaaagt ttgctttctt agcaaattcc
     7921 atttcttcaa aagcgtttac ctccttgtat cttataacat cagtatctga aaaagtacct
     7981 ttaaatggga ttaaaactgg tgcaaatatc ttgtagtctt tttttagata attcagacct
     8041 tcattgaact tagatgaatc taattttagt ttcattttta tcacctttct tatattttag
     8101 ttttacttac ttgctatctt gtttaaatct taacacttta ttttctatgc ttccgtgata
     8161 attatcacag tttcaaaaat aataaaaaaa atattaatgg agtttattaa gttctgttaa
     8221 tattttttta ttatctacat tattcgattt caaatctaat cacttaaata caatcattaa
     8281 gtacaataaa actactatct actcaatttt ttgaataaaa aaga
//
LOCUS       DAMJYX010000124         8246 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJYX010000124 DAMJYX010000000
VERSION     DAMJYX010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.129"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(77..625)
                     /locus_tag="PC479_003103"
     CDS             complement(77..625)
                     /locus_tag="PC479_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805624.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(728..1399)
                     /locus_tag="PC479_003104"
     CDS             complement(728..1399)
                     /locus_tag="PC479_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805625.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            1795..2700
                     /gene="garR"
                     /locus_tag="PC479_003105"
     CDS             1795..2700
                     /gene="garR"
                     /locus_tag="PC479_003105"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2805626.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDVKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            2836..4218
                     /locus_tag="PC479_003106"
     CDS             2836..4218
                     /locus_tag="PC479_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2805627.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            4289..5725
                     /locus_tag="PC479_003107"
     CDS             4289..5725
                     /locus_tag="PC479_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2805628.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(5911..6765)
                     /locus_tag="PC479_003108"
     CDS             complement(5911..6765)
                     /locus_tag="PC479_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805629.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(7033..7356)
                     /locus_tag="PC479_003109"
     CDS             complement(7033..7356)
                     /locus_tag="PC479_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2805630.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            7559..8188
                     /locus_tag="PC479_003110"
     CDS             7559..8188
                     /locus_tag="PC479_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805631.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
BASE COUNT         3099 a          981 c         1340 g         2826 t
ORIGIN      
        1 aaaataaaag actagttata atatttatat gaaaaagtac aaaatatagt atatgaatat
       61 agaaaattta aatcatttat ttacagtttt tatttccata agtatattta ccttcagata
      121 ccttgtcatc atggaaatat attctttcac aacttttatc tccccataga atgttttttg
      181 ttattttatg ggacgtatct cctgtaaata cttcagctac agtagtatct gtaacatcac
      241 agtaaagaga agcaaatttt ttaaaccaag gattttcctc aaatcttaaa agccaagaag
      301 ctccataggg gcaatgtaac tcttcaaatt caggaaccca tcctagaaat ggaacatcag
      361 ttgtgctaat aatattgtct gcgattaatt catctccatt ttcatgagct ctctctctca
      421 tagcaatagc tctttcttca gacctttcaa ataaaacttt tcttaatatt tcgctagttt
      481 tatcttcacc aaactcttca aataacactt tggaaaaata aaaatataaa tcaccaaaat
      541 gtttgcatgc catacgaact tcttcaatag cataatcttt gtcaatagtt tcattagtta
      601 caacatcttc gttcaaaaaa atcactcctt agaataagta ttagttacaa tataattata
      661 gaatattttt tataaattag gaaattaatt tgagatagat atatttttaa taaatgaaag
      721 tataatacta aaataactta ttgttacaaa aatcttctaa attaatgtat ttaggagttt
      781 ccatatgaat gagagtagtt ctgtaaatat cttcaataca tccatttgca atattattaa
      841 aaatatctgt gtgctctttt aatgttgttt caagccttcc ttcatgtgac aatgaatcga
      901 gagctaattt ttttattctg tacatataat tgttaaacat tttgtcaaat tcagtatttt
      961 gagcatatga aactatagtt gtgtgaaata atgtatcgta ttctgcaaat gagtttaaat
     1021 ttttatcacc taataaaata tctttttgtt tatctaaaag atgttttaat ttacttatag
     1081 tttctactgc tctagcttct ttgtattgac ttgtaattaa aaaagtacaa aatccttcta
     1141 ttgcagagcg tatttggaat atctctacaa tttcatttgc tgtaatttga tggattctaa
     1201 atcctttgct tgggattata tctataaatc catcttgact aagtctttga agagcatctc
     1261 ttataggtgt tctagagata cctatttcac tagcaagttt tgtttcagaa tatatttcat
     1321 tttctatcat ttctccagat agtatcaatt cttttaagta gtcatatgct tgttcttgta
     1381 atcttttaaa tgtagccata cttaataata cccccctaaa caatataaaa atacaaaaac
     1441 ttatataatt ttaagcatac aacatagtga aaataaggtc aatcttttgc aatttaaatt
     1501 gcagataaga ttaaaaaata taaaaaatta ttgaagtgtg aagcatgatt aacattaaag
     1561 gcagattaat taaaactaat tgaaattagt taaaatacaa aactgtaaaa gtagtatatt
     1621 atatttgaaa acaatagtat gtattaaaac ttatatagga tgactaaata taataaatga
     1681 ctttaaaaat tactatttat tgttaaaata aatactttta tattttatat tgaccggtat
     1741 actggtatgt agtatactct aaaacataaa aaacgttttc aaaaatgtga agatatggag
     1801 ggttataaaa tgaaattggg atttattgga ttagggataa tgggaaagcc aatggcaaaa
     1861 aatttattaa aagatggatg taatcttttg gtgtatgaca ttaataaatc tgctgtggat
     1921 gaacttatat catgtggagc aaaatacgca agtgttttag aaatgggtca agaatgtgat
     1981 attgtattta caatccttcc aaatggaact attgtgcagg atatattgtt tgggatggat
     2041 ggattggcaa aaactttaaa agaaggaagt atagtagtcg atatgagttc agttactcct
     2101 acagaatcaa tattgtgtgc aaataaatta aaagatatgg gcttagaatt tatagactca
     2161 ccagtaagtg gaggggagcc aaaagcgatt gatggtacac ttgcttttat ggctggtggt
     2221 aaggaagaaa tttataagaa agtagagcca ttttttaata taatgggaag ctcatctatt
     2281 ttaattggag ataatggtag tggaagtgtt acaaaactta caaaccaagt aatagttaat
     2341 ttgacgatag cagcagtttc tgaggcattt gtacttgcag caaaagcagg tgcagaccca
     2401 gaaaaagtat ataaggcaat aagaggaggc ctagctggta gtacaatatt ggatgtgaag
     2461 ataccaatga ttatgaatcg tgattttaaa ccaggtggaa aaatatctat taatttgaaa
     2521 gatattaaga atgtaatgca aacagcacac aatttagatg taccactacc tatgacaagt
     2581 caattattag aaattatgca gacattaaaa gtacatggac atttggaaga tgaccatagt
     2641 ggaatagcac agtattttga aaagttggca ggtgttgaaa taaaaaaaca cgataattaa
     2701 tatttaaata tatcaatata attaaataca tattattagg attagattat ataaatacag
     2761 cgaggttaag ttatcatagt aaaagaaaaa taaaattgat aaataataaa gtagaatgat
     2821 aaaggggtaa agattatgat tacaggatat ggacttttga tagcatttgt tatatctata
     2881 ggaattttac ttgtatcgat tattaaattt aaagtaaatc cgtttttggc attactgata
     2941 acatctataa ttacaggctt tatggttaga atgcctataa atgaaatatc aaccacaata
     3001 tcgactggat ttggaaatac attaggtagt ataggtattg taattggact tggaataata
     3061 tttggaaata ttctttcaga atcaagagca acagaatcta ttgcaaaggg gttgttagct
     3121 aagactggag aaaaaaacag tgctttagca gtaactacag caggtttttt aatttcaatt
     3181 ccagttttta tggatgcagc ttttgttata atgatgccta taattaagta tgtttcgaga
     3241 gtaactaaga aatctctaat ggtttttgta tgtgccttag gagttgggac tatagtaggt
     3301 catgcacttg taatacctac accaggacct ttggcagttg cagcaaatgt aaatgcaaat
     3361 gtaggtagtt ttatacttta ttctataatt gttgcttttc cagcagctct tataggtgga
     3421 tggatttatg gaaaacgttt tgaaaaatat ccagcttatg ctattgatga gaatgataga
     3481 gaaaaaaatt tagagcaggg aaaagatagc ataaaaattg aagatgatga ttcttctaaa
     3541 gtaccaggtt ttggaatttc tatgttttct ttattgttcc caatacttct tatacttata
     3601 tcaaatgtat ttagtatgtt cttagaaaaa ggttctacaa tgagtggagt acttgcattt
     3661 ataggagata aaaacatagc aatacttctt ggaatattgg tggcaatagg atttttaaag
     3721 aaatacataa ataaacctat gggagatgta gttattgaag ctgctgattc agcagggcta
     3781 atattattga taacagggtc aggtggtgcg tttggcagtg ttataaatgc aagtggtata
     3841 ggtaacttct tagttgatac tatgtctggt cttagtatat cagttgtagt acttggattc
     3901 ttattaagtg ccttgcttag aatttctcaa ggttctgcta ctgtagcctt agtaacaaca
     3961 tcatcaattt taggacctac aattttggca actggaatgt caccagtttt agtagggctt
     4021 gctatatgtg ctggaggagt tggtttctcc cttccaaatg attctggatt ctgggtactt
     4081 tcaagattta gtggattatc agttaaagat actttaaact cttggacaat tggaggaacc
     4141 attgctggag ttgtagcatt tataatggtt ttattgctta gtgttataaa tggaattata
     4201 ccacttccag gtctataaat aacaaactat tgtttatatc atataaatta tatttagact
     4261 atacaaatta cataaagggg cgttttaaat gagagaagta aaaccaatta gcatagatat
     4321 acttaatact tttaaacaag tagatgagga tagattaaat aaacttcttg cagatgagct
     4381 taagcattta gatagaaaaa ttgttgtatt agatgatgac ccaacaggtg tacaaacagt
     4441 tcatgatatt tctgtttata ctgattggga taaggatagt atggaacaag gctttaatga
     4501 gaaaaatagt atgtttttta tacttaccaa ttcaagggga tttactgttg ctcaaactac
     4561 aaaagcacat aaagaaattt caaagaatat tgtggatgta tcaaagaaag ttaataagga
     4621 ttttataatc ataagtcgta gtgattctac tatgagagga cattatcctc ttgaaactaa
     4681 tttattaaaa agcgaagtag agagattatc ggggaagtta tttcagggtg aaataataat
     4741 gccatttttt aaagaaggtg gtaggtttac tatagataat gtacattatg ttaaagaagg
     4801 agaaatactt gtaccagcag gaatgactga atttgcaaaa gataaatctt ttggatataa
     4861 atcatctgat ataggtgagt ggtgtgaaga aaagacaaat ggaaagtaca aatctagtga
     4921 tatgatatat atttcgtcaa aagaacttag agagctaaat attgaagcta taacagaaaa
     4981 acttaaaaaa gctgaaggat tcaataaaat tattgtaaat gcaaccgatt atgtagatgt
     5041 aaaggtattt acaattgcgt ttatacgagc agtaaatggt ggtaaggaat ttatgtttag
     5101 aagcgctgct gccataacaa aagtacttgg cggagtgagt gacaaggaac tacttactaa
     5161 ggatgaatta gtatcaaaag gtaataccaa tggaggaatt atacttgttg gttcacatgt
     5221 gaataagact acccaacagt tagaagaact taaaaattgt aaatatccga tagaatttat
     5281 agaatttaat caacatttag ttcttcaaga aaatggtcta aaaaatgagg taaaaagagt
     5341 tataaaagtt gtagaggaaa aaataagcag tggtaaaact gttgctgttt atactagacg
     5401 agagcgtttt gacttggata cgaatgataa agataaacag cttatgattt ctgtagaaat
     5461 atcagatgca gttaccagta taattggtat gcttaatgta agaccaaatt ttattatagc
     5521 taagggtgga attacttcta gtgatgttgg tactaaagca ctaagagtaa aaaaagctac
     5581 agtaatggga cagataaaac ctggtatacc agtatggatg acaggcaaag aaagcaaatt
     5641 tcctaatatg ccatatataa ttttccctgg aaatgtggga gagattagca cacttagaga
     5701 atcagttgag atattgatgg ggtagtatat tagtgtattg aagtttaaac gcttaaattt
     5761 actgagttaa aattaaaata aactaaaatt ataaattaag attaaaaata atagtatatt
     5821 aattagggtt tagagtattt tacaaaaaat attctaaacc ttaatcttat gtattagtat
     5881 ttttattatt taacttatgt gtttatttta ttatttatct tttttaattg atttaatatt
     5941 ttctttgatt ttatttaaaa tatctgtatg cttattagag cttatgttgg ggaatgcttt
     6001 gattatacgt ctatgaagta atttgtggtt agacagcaaa gtattaagtt tattttttag
     6061 ctcatcaata cgatttttag catagcgata attttctttt tctttaagta tttcaaacct
     6121 atcttccaaa gtatccagtc tattttcaac taaagattca agtttgtttt ctatagtttc
     6181 ttgtgtaata agtttgtagt attctttctt atcttttaat tccttaatta taagattgat
     6241 ttgttttaac ttaccattta attgtaaaat tgtatggact gtgataacta aatctaaaac
     6301 aaaataaatt tctatagata tagcaaatat aaatagcaca tttttagaga tagagttaat
     6361 aaagtcaacc acaataggat gtactataag cattacaatc aatgataata caccaaacat
     6421 aagtgagttt tccagacaaa ctcttccatg aatattaaat ttatgtttag aataatccca
     6481 ccaagtcata ttaaatattt tttctaaaat aaagctcgta cagtactcca ttatgctagt
     6541 tataattata ctaaacaaaa ataacaaagg aatattgtgt acaaaaggag tcaaggtagc
     6601 aactattgct aatgctccaa atccatatat agggcaaaat ggaccattta aaaaacctct
     6661 atttacaaat tgtttttgta aaactgaaca atatgtagtt tcacaacacc atcctataaa
     6721 gctataaatt acaaaataaa gaaattgaat ataaaatgta ctcataagca cctcatttct
     6781 agttagtata acactatata ctcgatattt tatttttatg attaatttac taaattcaat
     6841 ttacatattg attataccaa tttatagaaa tttatcaaat ttaattacag ttagaacatt
     6901 ataaacatgg ttataataat gaaattttat ttaaaataaa taagggtgtc aagtataatc
     6961 aaactaatag ttgtagatac tctacactat gttagtttaa ttacatttga cacccttata
     7021 tttatactat gactatttac attcgaaact agagcaacat gtttcaatat aaaggtttac
     7081 tctatcagtt tcaggagcaa ccaatatatt atcaagtgta cataagctac catctttgtt
     7141 gtatttacaa ttttttacag tacaaactaa ttcctcacaa tttttattat taacgttgct
     7201 agataaatta ctataaatag attcatctaa aaatgaacta caacaagtat gtctgtcatt
     7261 gtgagctttt tcaccattta taaaaatttt atctgcataa catatatggc tgttgttatg
     7321 tgaacagtta attacattac agtttattct ttccaaagtt attacctcct aaacgattat
     7381 ttattataaa ttatgtccat ataattacaa gaatataacc attaagagaa aactaccttt
     7441 tagaaaatat ctattcttag agtaatttga aatataaata tattttcttc taaaatttac
     7501 ctaaattgta taaaaactta cagcttggag aaaataatag aaaaaggagg cgtaatcaat
     7561 gtttgaaaag aagaaaaaag aattatttcc ttggaaaata tctgccttaa tactggttgg
     7621 agtatttgta ttaagtagcg gagtttatat agggacaaga ttaaaacaca atggagttca
     7681 aactggtgaa gttgtaagta ctagtgcgaa tatgaatgaa aagtcaaaag aagcttttgg
     7741 attaaataaa aattgtgaag tttggcttca aagtgaaggc gaaaatgaaa ctaactataa
     7801 gagaacactt acaatggcag gaatagttcc agagtcatta ttagataaga cgaagtcaga
     7861 aatagtatca tattttaaaa aagaatatcc aactaaaaaa ataaagagta tggataaaaa
     7921 tgaaatcata ttggtagaat ctaaatatag tgataataaa gatgtacaag caatggatgt
     7981 tactataaaa ggtaaatata ctattgaaaa tgataatgga aatatagcac tatataaata
     8041 tgatgatagc ggaagtaaaa cattagtaga aaaaacaaaa ataagagtag attcacttcc
     8101 taaaacagtt caagatgaaa tcaaaaaagg tgtagttatg gatactgaag aagaggccta
     8161 ttcaagatta gaagactttg caagttaata gtaaattaat attattttaa gttaaatact
     8221 aagtaaatta ataataaata atttaa
//
LOCUS       DAMJYX010000125         8171 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJYX010000125 DAMJYX010000000
VERSION     DAMJYX010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.130"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..597
                     /locus_tag="PC479_003111"
     CDS             <1..597
                     /locus_tag="PC479_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2805632.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC479_003112"
     CDS             728..1750
                     /locus_tag="PC479_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2805633.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC479_003113"
     CDS             complement(1914..2471)
                     /locus_tag="PC479_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2805634.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC479_003114"
     CDS             complement(2477..3553)
                     /locus_tag="PC479_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2805635.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC479_003115"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC479_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2805636.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC479_003116"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC479_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2805637.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC479_003117"
     CDS             5391..6320
                     /locus_tag="PC479_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805638.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC479_003118"
     CDS             complement(6481..6930)
                     /locus_tag="PC479_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805639.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="PC479_003119"
     CDS             complement(7133..8113)
                     /locus_tag="PC479_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2805640.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2824 a         1349 c          982 g         3016 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atttggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acgttataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtctatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataattgat t
//
LOCUS       DAMJYX010000126         8014 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJYX010000126 DAMJYX010000000
VERSION     DAMJYX010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.131"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(107..958)
                     /locus_tag="PC479_003120"
     CDS             complement(107..958)
                     /locus_tag="PC479_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2805641.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1007..2176)
                     /locus_tag="PC479_003121"
     CDS             complement(1007..2176)
                     /locus_tag="PC479_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2805642.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2647..3564
                     /locus_tag="PC479_003122"
     CDS             2647..3564
                     /locus_tag="PC479_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2805643.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(3693..4622)
                     /gene="pfkB"
                     /locus_tag="PC479_003123"
     CDS             complement(3693..4622)
                     /gene="pfkB"
                     /locus_tag="PC479_003123"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2805644.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4891..5616
                     /locus_tag="PC479_003124"
     CDS             4891..5616
                     /locus_tag="PC479_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805645.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5699..6853
                     /gene="nagA"
                     /locus_tag="PC479_003125"
     CDS             5699..6853
                     /gene="nagA"
                     /locus_tag="PC479_003125"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2805646.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(7562..>8014)
                     /locus_tag="PC479_003126"
     CDS             complement(7562..>8014)
                     /locus_tag="PC479_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2805647.1"
                     /translation="NILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVESKSLFIK
                     DRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPFGYEKNI
                     SLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
BASE COUNT         2815 a         1161 c         1020 g         3018 t
ORIGIN      
        1 tcatcaatta aatgaaaaaa atacaccatt tacatataat taatataaat ttttcatata
       61 ataaatatcc taaacttatc taaaataaat ttaggatatt ttatttttat actctattta
      121 cagacccaca cactttaatc ttttctttca caaaatcctt tactgattga agccctgcta
      181 atccatactt tttagggtct ataacctcag aatcttcatc taaaactttt ttaacccctt
      241 tactatatgc aattcttaat tcagttgcat agttaacttt tgcaatacct ctttttatag
      301 cttcttttat aatatcatca tgtattcctg aacctccatg caatactaaa ggtattgaaa
      361 ctacattttt aatttctgat aacctatcta aatctatttt aggttcaccc ttatacaatc
      421 catgtgcagt acctatagca atagccaatg aagatattcc tgttctatcg acaaactctt
      481 ttgcctcttt tggatttgta taatcattac tattcccgcc atctaaatca tcttcttttc
      541 caccaacttt cccaagttct gcttccactg gtataagaga aggactacat gcttttacta
      601 ctgatttaga tatagatata ttattttcaa agctttcatg agaaccatct atcattattg
      661 atgtatatcc acttcttaaa gctttcattg caaggtcaaa acttgaacca tggtccagat
      721 gcattgctac aggtatattt actctttctg cagcaacctt tgcatttgct agataataat
      781 caagacctgc atattttaca gtagatggag tagtttgtaa tattactgga gaatttaact
      841 cctcagcagc ttctataaca gcaattacca tttccatatt ttctacatta aatgctccta
      901 ctgcataccc ttcatcttgt gcctttttaa gcattgatgt tgttgttact aatgccacat
      961 tgataccctc ccaaattatt tagttataat attttaatct tatcttttaa tattcatata
     1021 ttgttacacc ttttactact cgatttactg ttcctgtagc tgatggagta tctggcttat
     1081 ttccaacctt tactgataca aacaatgcta tagtttgtgc aaacaatatg tatggcattg
     1141 caagatacac atctggtaat ttattatact tttctttaaa ctcaaacttt gttcctgaga
     1201 agttattttc attttctact gacactgcac aagtgaattt agcaatttta tcccttttta
     1261 tctcctctaa aacatctaaa tcatattttc ttgtatataa acagttagat acaaattcaa
     1321 atacaagtgt attttcatca ataaatgatt ttggtccgtg tctaaacccc ataggagaat
     1381 catacactgt agatattttt cctgctgtaa gttctaaaag ttttagttgt gcttcttgtg
     1441 tcaaaccacc taatcctcct gaacctaaat atacaactct atcaaaatct ttatttatta
     1501 actcatgtat ttcatctttt ctatctataa catttcttcc catttcaatt attgcattta
     1561 tatatgactt ttctacatca tcttctaaac tatcgaatat taacatagca gataacatca
     1621 tacacgaaaa acttcctgtc atggcaaagc cttcatcatt tgattttgat ggcattaata
     1681 acaagtaatt attttcgtcg tttttagtca tcttagccag ttctccttct ggattacaag
     1741 ttatagctaa atgataaaaa ttatctacaa tcttattccc taaatttaaa gctgctaaac
     1801 tttcaggact atttccactt cttgcaaatg aaattaataa tgttggtata tcttttttta
     1861 agtaatcata tgggtttgaa actatatccg tagtaggtat tgcttcaaat atgtattttc
     1921 ttatgtcatt tttattttta aggtaaggaa gtattgaatt tcctatgtaa gctgaagttc
     1981 cagcacctgt aaatataact ctaaactgcc catgattatt acttattgta tctatgaatc
     2041 tacttaattt ttctttgtta gatttatata tttcataagt ttctaaccat aattcaggct
     2101 gttgttttat ttcattagtt gttattattg cacctaaatc ttttagtttt tgttcttcta
     2161 atttaaacat tttcattctc ctttcatact tgtaacgttt acatttggta atgaggttat
     2221 aaccacttta aactataaaa aaatatactc ttttataatt ttttgcttag attttttagt
     2281 actacttaaa ctcactataa aatatacact atagatttta attaatttta tagtttatct
     2341 ttaaaaattt aataaaatca tactctatat actttagtat ttttatttta cgataaaagc
     2401 ttgttcagac caaagtggta attaccacta atccaaatat aaattaattt gaattttttg
     2461 tcaatacata aaatatttat atcaatcaat tttatggata tcctacaaaa ttactttatc
     2521 aatatttctt atttcgctaa tttacttata taagctttaa atttattttt tgatacaaag
     2581 tttacaaatt tatgtgtctg aattataatc aattctgtaa atataaaaac cttaggagaa
     2641 agtattatga aaaatataaa aatgggaatg ataggtcttg gctccatagc tcaaaaggca
     2701 tatttaccta tattaactaa gtctgagaga tttgagtttg taggagcttt tactccaaat
     2761 aaagttaaaa gggaaaaagt ctgtagtgat tatagaatca tgccattcga ttctattgaa
     2821 tccctagcta aaaaatgtga ttgtattttt ctacatagct ctacagaaac acactacgaa
     2881 attataaaaa tattattaaa tttaggtgtt catgtatatg tagacaaacc tcttgcttca
     2941 acagtaagtc aaggagaaga acttatcgag ttaagtacga aaaaaaactt aaatttaatg
     3001 gttggattca accgtagatt ctgtcctatg tacaaagaaa ttaaaaataa tgctacagaa
     3061 attgtatcta tcaatatatg taaacatgga ttaaattcct taagaaatgt tagattcgac
     3121 agtactctta tagatgacta tatacatgtt attgacactg ctttatggtt agcaaatgaa
     3181 gatgttgaaa ttagtggaga ggatttattt ttaacagaca ataagaatct tatatttgta
     3241 agtcataaac ttaaaggtaa aaatttctct ataaacacct ctatgcatag agattctgga
     3301 acaaagttag aacaagttga aattctttca aagggtaaga ttcaaagagt taagaatctt
     3361 aatgtactgg aaatagaaga aggtggaaac ctaactttaa agcaaagtgg tgcatgggta
     3421 aatatattaa aacaaaaggg ttttgaggat atatcaaatc attttattga ttgtatagaa
     3481 aataatataa aaccagctat taatggagaa gagtgtatca aagctcaaag attactagag
     3541 aaaattataa attcagtaaa atagatattt attataaaaa taggttaaaa atgaattttt
     3601 ctaacctatt tttatataag actatatctt ttatattaaa attaaaatct attacaacaa
     3661 cattaaattt attatattcc cattaaatgt atttaactta acttttttac ttcaactaaa
     3721 tcttgatatt tatcaatttc tcccaaatct acaaatccag tttccatatt taaagcatta
     3781 cttataccac aagcatttgc aaatttaagt aaattttcta ttttataacc ccttaaaata
     3841 cctacactaa atccagccac tgtgctatct ccactaccta cagtatttat acactttaca
     3901 actggtactt ttacatcata tacattatct tcgtttaaat acaccatacc atcttttcct
     3961 aaagatatac atacattctc tgcacccatt tcaataagtt tttttcctga caagataatt
     4021 tcctctcttg attctatatt cattcctaat aaatgtctta tttcatctat atttggtttt
     4081 attaaatatg gttttgattt taaagctatt tttaatgctt ctcctgatgt atctaatata
     4141 aactttatat tatttgctct acatatattt cctatctttt cataatagtc tatcggcata
     4201 ttctcacaat aactaccaga agctaccagt attttagtat tttttagtac ttcctctata
     4261 tcattttcaa atctctctaa atcgcttttt tctactattg gtcctttttc taaaaattct
     4321 gtactaactt tattgtcctc aattatgttt aagcaatttc tagtttcctg ctctattttt
     4381 gtaaatttat tttgtatatc tagttttcta agttcatttt ttataaattc tccattaaat
     4441 ccgcctagaa atcccatagc taaaggattt tcttttaaaa ttttacatac cttagctacg
     4501 tttaatcctt tacctccagc agttgcattc ttacttacta ccctttgcac ctcacctatg
     4561 tttatattat tcactctata cattctatct atagatgggt tgaatgttat tactgtaatc
     4621 atactagatt ctcctcatta ttaattttta cttttctaaa caataaatta atctgtaaaa
     4681 taaaaaatac ctcatctata atatacccaa agtggttatt accaatttga atataaatta
     4741 ttttacttgt tttgtcaatg taccataatt ttataataaa ttaataacct tacttattga
     4801 attttgatta aactttattc attgacataa ataattctat attctataat caaattgtgg
     4861 taataaccac ttagaaagga ctatattttt atgataaaaa gaaatgataa aagaccaata
     4921 tatgaccagc ttgttgagat attaagaact aagattgaaa atgagatgga accaaacgat
     4981 agaatgcttt ccgaaagaaa aatttgtgat gaatatggag taagtagaac cacagttaga
     5041 ctagctatgg ctgagttgga acatatgggg tatatataca aaagacatgg aaaaggaact
     5101 tttgttgctg cactaagtca aaattctcaa aacttaatgg aaagttatag ttttacagat
     5161 tatatgagaa cacaaggaaa aaagccaggt acaaaggtat tatcatttga ggtagtagaa
     5221 agtacaaagt atttctctga aaaattaggt ataactcctg gtgaaaaaat gataaaaata
     5281 atgcgtttaa gacttgctga tgaccttcct atgatgcttg aaagaacata tcttcctatg
     5341 aaagaattta taggattgac aaaagaaaaa atcgaacaaa aacctttata tgaaatattt
     5401 agagaagatt atggcgagat tattaaagtt gctgatgaag aattttcagc tggaataatc
     5461 tctgataagg aagctaagtt attggaagta ccaggagatt caccttgtct gaaattactt
     5521 cgtaccacat ataatgatga caatatagtt attgaattta ctttgagtgt tgctagaagc
     5581 gataaatttg tatatagagt aagacatgta agatagtaag ttaaataact ttcaagaaaa
     5641 tagaatgata ttataaacgt caatgccaca atgatagtat aattataagg aggttatttt
     5701 gaaaagtttt atttatgcag ataaattttt tatgaaacat ggaatcaaag aaaaaggtta
     5761 ccttagtata attaatggca aattcggtga atttcaacaa gaaagaccta atgttactga
     5821 taatgttata gatttttcag gtaaatatat tgctcctggc ttagttgata ctcacattca
     5881 cggtctttta ggtgcagatg tcatggacaa tacatttgaa gcaataaaca caatttcaaa
     5941 aggcttatta aaatatggag taacatcttt cctacctaca actttaactg attctgtaga
     6001 tacactaagt gattctgtag aaaatataag caatagcttc aatcgagttg agggtgcaaa
     6061 aatacaaggt atttatttag aaggtccatt ttttacagaa aaatataagg gcgctcaaaa
     6121 tgaaaaatat tttaaagacc ctgacatcaa tatattaaaa ttatggcaaa aaaaatcgaa
     6181 aaatttaata agaaaaatag caattgctcc agaacgtaaa ggttcaatag aattaacaag
     6241 atatgcaact aaaaataata tatcagtttc tcttggtcat agttcagcta catttgagca
     6301 agttaaacaa gtcgttaatt caggtgctaa agtgtttgta catacatata atgggatgag
     6361 tcctttaaat catagagaac ctggaatggt tggggctgct atggtactaa aagatactta
     6421 tgctgaatta atttgtgatg gtcatcatgt aagcccaata gctgttaaaa taatgatgga
     6481 tgcgaagtct agtaataaca tagctttaat tacagattgt atgagagctg gtgctatgga
     6541 ggatggaaaa tatactcttg gtaagtttaa tgtaaatgta aaaaatagtg ttgcaagatt
     6601 aaatagtggg agtttagctg gaagtgtatt aactatggac aaggctgtga gaaatatagt
     6661 tgattggaac atatcaactt tagaagatgc aataaaaatg gctacatatg tccctgcttt
     6721 aagttgtaat attgataatg tctgtggctc tatatatcaa ggtatggatg cagactttat
     6781 agtaacagat aatgacttta atatacacga aacttatata aatggtatct gtaagtacaa
     6841 agttaataaa taggttcaat ataaggttat ggaggctgtc taaaaagagt ctggaaatat
     6901 ttaaagttat ttctccaaaa tctgatttaa cacttttatt tccatatcgt taataaacta
     6961 ttgcgataga ggttatttta aacaaatata tattataagt atttttgtga taatattttc
     7021 gataatatgt acatttaaag taacttttct attattattt gtaagttttt atcttgagca
     7081 aacatataaa tttttaataa agcatgagat aaaccctata tttcaattac taatgatgag
     7141 attttgattg ataacagata tattttcaaa tgtaaaaata ctaaaaatca tatcaatttt
     7201 aaagatattg taggtgtaaa taaacaagat aatactatag aaatttttac aaatgaaagc
     7261 acttattgta tatatctaaa agttttaagc attgagagcg aagaatatct aatagataga
     7321 ctaaaatcat tttgttgatt ttatatattt tatgttaaat ataaagcaga gtagttaaaa
     7381 ctaccctgct tttgtaatta aaatttaaaa ataataactc ataatacatt gaatttgagc
     7441 ttaacagtaa ctattgtaat aatagtatca atcttttgtg ttgaatataa tttatttaca
     7501 ctatagttaa gcatgatttc taatgttatt ttctctaatt tacaattatg attaaaattc
     7561 attaatatat atatttataa gttgtattgt ttatattgtt tattactttt ttaaatccac
     7621 tagctgtaat aataaatgtc ttttctggta tccctggtga aaagattatt ttatcatggt
     7681 taaatatatc ttgatctatt acaagagaga tatttttctc atatccaaaa ggagatacac
     7741 atccaggcaa acattgtgtt ttttgtgtta attcttcttt tgaacatata gatacttttc
     7801 cacctaaaac attttttata gtttttggat ctaatttttt tccttctgtg gttacaaata
     7861 tatagtaatt tccagatcta tcttttataa ataaactttt actttctact ccagtatatt
     7921 gtaatttttc tcttatttct ttagcttttt caaatgataa aataggttca tggtcatatt
     7981 cttcaaacgt tatatgattt tcttttaaaa tatt
//
LOCUS       DAMJYX010000127         7911 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJYX010000127 DAMJYX010000000
VERSION     DAMJYX010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.132"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(391..2670)
                     /locus_tag="PC479_003127"
     CDS             complement(391..2670)
                     /locus_tag="PC479_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2805648.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2941..4395)
                     /locus_tag="PC479_003128"
     CDS             complement(2941..4395)
                     /locus_tag="PC479_003128"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2805649.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4559..5428
                     /locus_tag="PC479_003129"
     CDS             4559..5428
                     /locus_tag="PC479_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2805650.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5641..7713)
                     /locus_tag="PC479_003130"
     CDS             complement(5641..7713)
                     /locus_tag="PC479_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2805651.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2700 a         1300 c          836 g         3075 t
ORIGIN      
        1 atagaattta aaaataaaag aagagtattt caaatgattt gtataaatta ttatgaaaaa
       61 ctcttttttt gatataataa aattacttac atatataata tttaaattta taaattatat
      121 ttttattttt taaaatatgt ataacacacc tatagaaatt actattatta ctatctaaag
      181 tattttgcta taatcccata tactcttacc tcaaaaacat tcaaatgtaa aaaatactac
      241 atattaataa actttgccca ataattccta aaataatatc ctaacaatga tttatgtttt
      301 tataatagta tatttttaca taaaaaagtg aacattgatt aacaaagttc acttttttat
      361 aaatttttat gttataattc acaattttat ttaatttgtt tcttttctat ttaaactata
      421 tctatctaat attaattttt catattcttc tattggcatt ggtctagcaa ataaaaatcc
      481 ttgggccata tcacaaccta taaaccttaa aaactctgct tgctctacag tttcaacacc
      541 ttctgaaaca acttttatat caagttgctt tgccatttga acaatacttg aaactacaat
      601 ttgactcttt tttgcatttc ccactgttat aaagaattgc ctatctaatt tcaatacatc
      661 aattggcata tctttaagca tatttaaaga agaataacca gaaccaaaat catccataga
      721 aattttaaat ccaacagatt tcaaagtttg cattatttta aataatattt ttgtattgtc
      781 aaatattgca ctttctgtca attcaatctc aattaaattt ggttcaattt catattcttt
      841 tgttattctt tcaagctcag aaataaagcc tggattctca agatgtactc ttgataagtt
      901 tattgatata ggaaatgtgg ggaaattttc attttcccaa cgtttaaaat ttttacaaac
      961 ttgggtaaat acaaacatat ccaattctgt tataaaacca tttttctcaa aaataggaat
     1021 aaatacacct ggacttatta gacctttttt gggatgttgc caacgtacaa gtgcttcagc
     1081 acctacaatt ttagtagttg ataaatcaac cttaggctgt aaataaactt taaattgttc
     1141 attttttaaa gcatcatgca tttcttgttc aatatcacgt tcttctatga gagtttcttt
     1201 taaattttca gcataaatct cataaacttt taaatatttt ctttttactg tagtttttgc
     1261 aatcatagct ttgtcaagac aggtagaaat atttgtttct ccctcttcaa caaaatatac
     1321 acctatggat ggaattaaat tgagttctac tcccaaactt gtttttatca ttgacaattt
     1381 tttacgtata agttcacata tttctataat tgactctcta ttatttttct tttcaaatat
     1441 tatagcaaaa ttgtcatttg aaattcttga aaatacagat tgctctttaa atatattctt
     1501 aattatctct gctatttcct ttaatactct atctccttct tcatacccaa acctatcatt
     1561 tactagctta aacttatcta tatcaaaata aattaaaact agattctttt tctcatgaga
     1621 ggataaaaat tttccccctt caagattaaa tttatttgaa ttgccaatac ctgtaacgtt
     1681 atctttaaaa gcaaattctt ccaatttttc cttactcttt ttatgtataa atagtatata
     1741 aattgttaat gccgtaaaga ttataatcaa gactacacat gctgataatg taagttttat
     1801 aatagcatat gatttctcag atatagcagt tttaggaact tcagaaaaaa gataccaatc
     1861 attgattcct attggggcat aacttaaata atatcctttt ccaaacatac tataacttaa
     1921 tgaacctgat ttaaagtttt ttatatttga cttcatattg ttgatactag attttgatat
     1981 tgaatcactt ctgccaatgt aagaaaataa attttcttta gtagagttgg cgtttttact
     2041 gcttggattt aaaacaatat caccattact tctagttata taagaatatc cttttccatt
     2101 aaatgtaggt acagaaagta ttttttcata caacttcgta ctatgtgtgg caaatataac
     2161 tcctttaacc tcattatctt tataaattgg tacagaatat acattaatat tactattggt
     2221 atttttatca tcttctatta catcttttaa ttttcctgtt ataaccactt ctccttgcat
     2281 tgattttaag aaataatctc ttttagagaa gtcttctata taaccatctg tagtgtatgc
     2341 tgtaccattt ggtaaaataa ctcccattct tttaaatgaa ctattaatag cttgtctttt
     2401 tagtatagaa aggatattat ctacttcaaa agactcttct ccttcaataa atattgaaat
     2461 tgactctagt aactttatat ctccattaat ttgttttttt aacacattga cgctttgatt
     2521 tgctatttct gaaagatatt gattcgtttc ttcacctaat gtcttattca accttgaacc
     2581 ataaatcaaa caggaaattg taattactaa gatacttatg acagcaatta tggacaaacc
     2641 atacctatta ttttttgcat cacttaacat tatgcttcct gcctttctcg aatctatgta
     2701 tgtataatca ttatcttatg acaattattg taaacatttt ataataatac ttatttttcc
     2761 atatttactg taattataca aaattataaa ctttattaca acaataaatt aattaatata
     2821 gatattagtg tttacattat tgcattatct attttacaat tatttacata taaaaaaatg
     2881 taatatcaaa gtgaagcttg atattacatt tttgttcttg tattttgttt aatttttatg
     2941 ctacatctta cttaaacact catttgcaac ttctttatca tcaaaatgat gcttaacttt
     3001 acctataatt tgataagttt catgtccctt acctgttata attacaacat catccttttt
     3061 tgccatactt atagcttctg ctatagcttg ttctctatca acaacaacat ggtaattttc
     3121 ttttttcttg tcaattcctt caagtatatc ttttattatt gcctctggtt cttcagttct
     3181 tggattatca gaagttataa tacataaatc agaatatttt tgtccaattg ctcccatcaa
     3241 aggtctcttt tcagtatctc tatctccacc acacccaaac acagaaatta tttttccttc
     3301 tgcaaattgt tgagttgtct ttagtacatt ttcaagtgcg tcaggagtat gagcatagtc
     3361 tacaattacg cttatacctt tatcatttgg aactgtttca aaccttcctg atactccatt
     3421 agaaagtcta aggccttctt tatatattgg tttaggtatg ccaagtacat agcaagcagc
     3481 tataactgct aaagtattat atactgtaaa catccctgga actggtataa atattacttc
     3541 ttcatatgaa ggtgttatta atctataaga tactccatcc gaatctgatt ttatatctct
     3601 agccataaaa tcagccttag tatcaacacc ataagtgtaa caaggtacat ttataccttt
     3661 tatattttca tatatctttt ttccaccttc atcatctatg tttattatat ttgccatagt
     3721 agtcttaaaa aataattttt cttttgcttt tctataatcc tctaaatctt tatgaaaatc
     3781 taaatggtct ggtgttaaat ttgtaaatat gcctagttta aagtctgttt catcaactct
     3841 atttaatgcc aatgaatgtg atgaaacttc cattgcacag tagtcacatc cattatccaa
     3901 catattatta aaatgatatt gtaaatctat actttctgga gttgttgaat tggaaacaat
     3961 gtctttttct ccatcaaaaa tttttatagt tcctataagt ccaactttat tcttatttaa
     4021 agttaatatc tcatttagta tagtagttat actagttttt ccattagtac ctgtaactcc
     4081 tataacgtta aatttttgag atggatgatt gtaaaaatta tcagctactt ttgccatatc
     4141 ttttcttgta tcttttacct ttataaatgt acatcctttt attgctacat cttcttcaac
     4201 aagaaatgca cttgctccct tttctatggc atcttttata tacttatgac catctgacac
     4261 aaatcctttt atacatataa ataatgtacc ttttgtaacc tttcttgaat catattgtac
     4321 attattaata tctatattta attcaccctt gacattgatt atatccaacc cctgtataat
     4381 atcatttaat ttcataaatg ctttcctccc tttaaactct ttctgctaaa attacactac
     4441 atataatatt atattaagaa ttttttacta tgtgaatggt taaaaatgct tgaaacattg
     4501 caaaaaatgc tctataattt tctataatat aactgaggaa aggtcttcta taaaaactat
     4561 ggataataaa atcgggtact taaacgataa ctttaaaata tttaatatta gagataaaaa
     4621 agatatacaa tttgaatacc ataatcatga ttttaataag ataattatat ttataaatgg
     4681 aaatgtaact tattttgttg atggtattcc ctataaacta aagccttggg atatactctt
     4741 tatcagcaat aatgaaatcc ataaaccagt tatagattca aatgtctttt atgagagaat
     4801 tgcaatatgg attagcccag aatttatgaa aaaaaatagt gatgctagta gtgacttaga
     4861 aaaatgtttt aaaatagcta cagagaataa atgcaataga ttgcgtttaa atatgcatga
     4921 tattgaatct attaagagtt ttattgagca aataaatatt tccgaatcca atactgaatt
     4981 tggaaatgaa atattgagaa atacattatt tatacaactt atgatattct taaacagaat
     5041 tttcctaaaa aatgaaaata tggaatcaac agaagatgtt ttttatgatg aaactattaa
     5101 gaaagtatta aactatataa attcaaatat agatgataag ctttcaatag atagcatatc
     5161 gtctgaattt tttataagta agtattatct tatgagaaaa tttaaatctc atactggaac
     5221 gtctatacac aattatatag ttcaaaaaag attaattttg gcaaagtcac taataaccaa
     5281 tggtcacctt atgagtgaag tttgtaataa atgtggattc aacgattatt ctagttttgt
     5341 aagggcattt aaaaaaaact acggtgtatc tcctaaaaat tatataaaca ataaaaatcc
     5401 tttagatatt actcttttag atgaatagat aatttatgta aattttattt taataaaact
     5461 tgaagtatta gtgtatttat cttactaata cctcaagttt tttaatgttt taaaatatat
     5521 caaaatataa tgatatatat ttctattact taaaatatat attaaataaa ttgcatgtta
     5581 actgaaccta tttatatatt tattaaatct cacataactt ttagttacac tatcatatag
     5641 ctatcctatt ttttcaatat catctcttaa tatacttact ctattctcct cttttgctgt
     5701 ggttactcct tttttaatgc ttaatttctg attttgttct aaattcgtaa atactacagg
     5761 acttattgtt gatagaccat ttttttcaat atattctaag tccattttaa ctaatttttc
     5821 tcccgctttt acttcatctc ctacattcac aaatacttca aatccatttc cttctaagtt
     5881 tacagtttct atccctaaat gtataagtat ttctactcca cttttttggg attttattat
     5941 tattgcatgc ttagttggaa atataactcc aatagttcca tcaactggtg aataaatcaa
     6001 tccatcacta gatttaattg caaatccatc ccccatcagt ttacttgaaa atacagaatc
     6061 tggcacttct tctatatcta ataactctcc atttgttaat gaatcaatct tttctactaa
     6121 agtattttcc atatttgcaa cttccatatt ttttttcact ttatttttat caacattttt
     6181 agtaacagta ctttcaacct ttgtttttcc ctctattata tcttttatat cagactttat
     6241 tgaatctgat tttggtccaa atatagcttg aacattgttg ccaataacca taacacctgc
     6301 tgctccaagt gatttcaatt tttctttatc tactttattt atatcattta caaccaatct
     6361 aagtcttgtt atacacgcat ctagactctt taagttgtct ttatttccta aagcaatcaa
     6421 aatctctgat gctaattcat tcttagacat ttcaatgact tcagcgttag tttctgactc
     6481 atcttctctt cctggtgtct ttaaattaaa ttttcttatc ataaatctaa atacactata
     6541 gtataatact gcaaaaccaa tacctaccaa gatagccaag taccatggag ttctattagg
     6601 aagaacacca aataataaga aatctattaa tcctcctgaa aatgtgaatc caattttaac
     6661 tcctaataat tgcataattg cgtatgataa acctgaaaat aaacaatgta ttgcaaataa
     6721 tactggtgca acaaataaaa acgcaaactc tattggctcg gttattcctg ttaaaaaaga
     6781 ggttaatgct gctgatgcca ttatacctgc tacaaatttt ttcttttctg gctttgcttc
     6841 atgcaccata gcaagagctg ctgctggaag accaaacatc ataaatggaa atgcacctgt
     6901 cataaaagta cctgctgtga aatttactcc atctttaagt tgagcaaaga atatcttttg
     6961 gtcacccatt acaagctcac cagctttatt tacatattct ccaaattgat accaaaatgg
     7021 attatggaaa acatgatgca aaccaaatgg tattaatagt ctttccaaag aaccttccat
     7081 aaatgctgct agtgtttgat ttgcttctat aacatttttt gagaaactaa gtaatccctc
     7141 ttgtattgga ggccaaataa aagacaatat aattcctatt gctatagcaa ctaatgaagt
     7201 aacgattggt acaaatcttt tacctgcaaa aaaacctaga tactgaggta attcaatttt
     7261 ataaaacttt ttataaagat aagcagccac tatccctata ataataccac caaatacccc
     7321 tgtttgaatt gttggtattc caacaatatt tgcatacata gaattgtcta ccatatcttc
     7381 tgtaacacct gccataacac ccatagatgt attgagaatt aaaaaaccta ctatggctgc
     7441 caatgctgca acaccttctc cttcagttaa accaacagct accccaactg caaatataag
     7501 tggtaagtta ttaaatataa tttgacctga actttccatt atacttatta taagttgaaa
     7561 tgtaggattc gccaaagctg gaaaatgagc caacaatgcg tctcctgcta aagcattact
     7621 cacgccaagt aatatacctg ctgcaggaag taatgcaact ggtagcatta gagattttcc
     7681 tactttttgt aaaacaccaa atactttttt catttacttt tctctcctta ttattttata
     7741 tttttttaaa taaaaaagac ttaagtttat aaaaagttac attagtatac ttaattctaa
     7801 aattaaactt atgttaatat aaacaaacct atattaacct aatttcagaa ttaaaaataa
     7861 actattataa tttttttata aacataagtc ttgcctgcat tatcagtaac a
//
LOCUS       DAMJYX010000128         7723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJYX010000128 DAMJYX010000000
VERSION     DAMJYX010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.133"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            41..1099
                     /locus_tag="PC479_003131"
     CDS             41..1099
                     /locus_tag="PC479_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2805652.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1203..2387
                     /locus_tag="PC479_003132"
     CDS             1203..2387
                     /locus_tag="PC479_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2805653.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2594..3307
                     /gene="rpsB"
                     /locus_tag="PC479_003133"
     CDS             2594..3307
                     /gene="rpsB"
                     /locus_tag="PC479_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2805654.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3386..4297
                     /locus_tag="PC479_003134"
     CDS             3386..4297
                     /locus_tag="PC479_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2805655.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4382..5086
                     /locus_tag="PC479_003135"
     CDS             4382..5086
                     /locus_tag="PC479_003135"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2805656.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5112..5669
                     /gene="frr"
                     /locus_tag="PC479_003136"
     CDS             5112..5669
                     /gene="frr"
                     /locus_tag="PC479_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2805657.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            5672..5875
                     /locus_tag="PC479_003137"
     CDS             5672..5875
                     /locus_tag="PC479_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805658.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5904..6638
                     /locus_tag="PC479_003138"
     CDS             5904..6638
                     /locus_tag="PC479_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2805659.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6651..7430
                     /locus_tag="PC479_003139"
     CDS             6651..7430
                     /locus_tag="PC479_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2805660.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3179 a          782 c         1461 g         2301 t
ORIGIN      
        1 aaaatagtaa taattgtttt gtactggagg atatatattt atgaattgta aatggatatc
       61 taaaatagat gagagaaaaa aatgtcatag agaagcagat tcttctggat attgtatctt
      121 tcataaagaa aataagtctg atgaagaaat acagttgatg atggatacta ttcataaaga
      181 agagataagt gaatttaatg gatttgtttt tgaaaatgaa tttaatgcgg aagaaatatt
      241 aacttacaat tataaaatac tggatttttc tgaatccata tttaaacaaa aagcaaattt
      301 taaaaaatat atttttaaaa agaatattat ttttaactat acagaattta gagataaagt
      361 tttatttaat ggttgtgtat tcttagaaaa ctgtgatttt aatagaagca tatttagtaa
      421 aaactatata aatgaccgta tatttgaaaa agtgaaattt aaaggacctg atttggtagt
      481 taataaagta gaaaattttc caagaatgga tggaataatt tttagcatgt gtacaaaatt
      541 tgtgttaaag aatgttgaat atggaaaaag tgagtatgag catggcaaga taaattatag
      601 aatagctaga aatcaagcaa caaaaattgg agaatatgag atgataggat tttactatta
      661 taaggaaaga atttatagta gtaaaataat gaaacgttct aattatccta ctttcagtga
      721 ttatttagtt gaaaaatttt ttgaccaaat agcacgttac acaacaggtt atggagaaaa
      781 gccatggaat atattattag ttattatagc aataataagt gtttttgctt tattatactt
      841 atttgtaggc atagaaagtt caaatagcac acttgtagct ttagatataa ataatatagg
      901 tgattactca ctaagtgaga ttttcaaaat gtatatggat ttatggtatt ttagcatggc
      961 tacatttagt acagtaggat atggagatat ggttgctaca agtttgattg ggaaagcatt
     1021 ggcaggtata gaagtatttt ttggagttac aataggagca atttgggcat ctgtgattat
     1081 taaaagaatg ataaggtaat atttgtgtaa atatggatag gtattaatta ctagatataa
     1141 attggactaa atagggaaaa tttaaatata aatatactta ataatccaag aaggtgaaaa
     1201 atttgaaaaa tgcactaaaa aaaattatat cattagccat gattctagtt tttataatac
     1261 caaattattc atatgcagat gataaatctg tagacaagta ttcaaaaaca tctatactta
     1321 tagaccaaga aacaggtaga gttttatata gtaaagaacc tgatatgaag gtaccattag
     1381 ctagtttaag taagatgatg acttttttac ttgcaataga agctattgaa aataaagaag
     1441 taaaagaaac tgataaagta aaaatagaca aatctattgc atcagtaaaa gggtcaagtt
     1501 ataaacttaa agatggtgaa gaagtgccac ttatagagct tatgagaggt cttatgattg
     1561 tatcaggaaa tgatgcggca attgctatag caaaacatat ttgtaaaacg aaagaagcat
     1621 ttgtagatag aatgaataaa aaagcaaaag aaataggaat gagtaataca cattttctaa
     1681 atccaaatgg tcttccaata tacgatttaa gcaaccccaa aaaaccagca aaagaaaata
     1741 tctctactgc aaaagatata gctattcttg gaaaatacat gtttgaccat tatgaaaaac
     1801 aagtaacagc tataactgat atggagacat atacaaattc agatagaagt tttgaaaaaa
     1861 gcaatacaaa tgcattatta agaattatac cagaagtaga tggtataaaa actggatata
     1921 ctggcaatgc tggatattgc ttatcatttt ctatgattgt aaataaaaat gataaaaatg
     1981 agaaaaaacg tagagtaata ggggttgtat taggtactaa ccataaaaat aaaagaacct
     2041 ctgcttcatt agctctactg aaatatggaa aagaaaattt caatatgaaa aaagtaattg
     2101 ataaagatag ctttattggt aagaaatata taaaaggaat gaaagagtta gaggttacat
     2161 taaaagcaaa agatgaactt tatacaatat taaaagatga tgaaagtata aaatcagaag
     2221 ttaagattaa aaatatagag taccctgtta aaaaaggtga tactatgggt attataaaat
     2281 attatactaa ttcaggtgac ttacttggaa gtgtagatat agtaagtaat aatagtatag
     2341 ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc aaattaaatt caaaaaatgg
     2401 tataaaatac acaaaaaaat ataaaaatag aaaatatgga aaaaatattg ttatttcttc
     2461 acctctgtga tatactacta gaggtgaaaa aataaaataa atcacacaca gcattgggat
     2521 ccttaaaagg tgcctattac atggtttttt agggagaaga tcaagctgga ggtataaaac
     2581 caggaggtaa gatatgtcag taatatcaat gaaacaatta ttagaagcag gtgttcactt
     2641 tggtcaccaa acaagaagat ggaaccctaa aatggctaag tatatattca cagagagaaa
     2701 tggaatatat ataatcgact tacaaaaaac agttaaaaaa gttgaagaag cttacaagtt
     2761 cactaaagaa gtagctgaaa caggaaagcc aatcttattc gtaggaacta aaaaacaagc
     2821 tcaagatgcg ataaaagatg aagctgaaag atgtggaatg tactttgtta atgaaagatg
     2881 gttaggtgga atgttaacaa accacaaaac tataaaaaca agaataaata aattaagaga
     2941 attagaaaaa atggaagaag aaggcgtatt taatgttctt cctaaaaaag aagttataaa
     3001 attaagagct gaaaaagaaa aactagaaaa atacttaggt ggaataaaag acatgcctga
     3061 attaccaggt gcaatgttcg tagttgaccc aagaaaagaa aacatagcta ttcaagaagc
     3121 tcatagatta ggtataccag tagttggtat agttgatact aactgtgacc cagaacaatt
     3181 agactttgct ataccaggaa atgatgacgc tataagagcc gtaaaattaa taactggtgc
     3241 tatggcaaca gctgtaattg aaggtagaca aggtgctgag gaagaagtag ctgaagacca
     3301 agaataataa tttgacaatt aaatggtaag ggtcgccctt accatttata atatggaata
     3361 gaataagtag gaggaaactt aaaaaatggc taatataact gctcaaatgg taaaagagtt
     3421 aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa gctttacaag aagctgaagg
     3481 aaacatggaa aaagcagtag atttattaag agaaaaagga ttatcaaaag ctgctaaaaa
     3541 agctggtaga gttgctgctg aaggtctagt tgcaatagaa atgaatgatg ataatacagt
     3601 tgcttctatg gtagaagtta actcagagac agatttcgtt gctaaaaacg aagacttcaa
     3661 agtgttcgtt aaagatgctg cttgtatggc attagctact gataaagaag atatagcttc
     3721 tttattaggt gaaactcata aagagggaat aactttacaa gaagttttaa ataatagagt
     3781 tgcaaaaata ggtgagaaat tagactttag aagatttgct aaagttgtaa ctaatggaca
     3841 agttgctgga tacattcacg gtggtggaaa aataggtgtt cttgttgaaa tggaaacaga
     3901 agctagagat gctaaagttt tagaattagg taaagatgta gctatgcaag ttgctgctat
     3961 gaatcctaag tatgtttcaa gagatgaagt agatgctgaa tacatagcac atgaaacaga
     4021 agtattaact caacaagctt taaatgaagg aaaaccagct aatatagttg aaaaaatggt
     4081 taaaggaaga ttagaaaaag aattaaaaga ggtttgttta ttagagcaaa ctttcgttaa
     4141 aaatccagat ataactgtta aacaattagt tgctgatgtt gctaaggcag taggttctga
     4201 tataaaagtt gttaaagttg tgagattcga agttggtgaa ggtatacaaa agagagaaga
     4261 aaactttgca gaagaagttg ctaagcaact taagtaataa ccaaagagag aacacgcagg
     4321 tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag gattgaaata ggaggtttct
     4381 gatggataaa ccaatgtaca aaagggtatt gttaaaatta agtggagaag cattagcagg
     4441 agaaagaggt tttggtataa ataatgatgt agttaatgat attgctattg ctataaaaaa
     4501 aatacaagaa ataggtgttg aagtagctgt agtagtaggt ggaggaaact tctggagagg
     4561 cagaaccagt gaaggaatgg acagaactac tgctgactat ataggaatgt tagctacagt
     4621 tatgaatgca atggcgctac aggatgcact agaaaatata gatgttgcta caagagttca
     4681 aactgctata gacatgagac aaattgcaga accatatata agaagaagag cagttagaca
     4741 cttagaaaag gaaagagttg ttatatttgg tgcaggaaca ggaaaccctt actttacaac
     4801 agatacaact gctgctttac gtgctgctga gatggaagca gaagttatat tgttagctaa
     4861 aaatgttgat gcagtatatg acaaggatcc aaaagtacat gcagatgcta agaaatttac
     4921 agaattaagt tacatggaag ttatacagaa agaattaaaa gtaatggatt ctacagctac
     4981 atctttatgt atggataata aaattccaat aaaggtattt gagcttacaa cagagaatat
     5041 aataagagca gtaaaaggcg aaaatatagg aactactgta aaataatgaa gaatgaagga
     5101 ggagtaaaat tatgaaactg gaaatacata agcaattaga agagaaaatg aatgggacaa
     5161 ttgatgcttt aaagtttgaa tttggaacta taagagcagg aagagctaat gcacaaatgc
     5221 tagacaaaat aagagttgat tattatggaa ctccaactcc aataaatcaa ataggtgcta
     5281 tatctgtacc agaaccaaga atattaatga tatctccttg ggataaatct gctatgcatg
     5341 aaatagaaaa agcgatagct aactcagact taggattaaa tccatcaaat gatggtgagg
     5401 ttataagact ttctgtacct gctttaacag aagaaagaag aaaagaatta gctaaaaaag
     5461 ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa tgaaagaaga gacgccaatg
     5521 agaaaattaa aaagatggaa aaaggcggag aattaactga agatgaatta aaaaaagcac
     5581 aagatgaagt tcaaaaaatg actgataaat ttataaaaga aatagatgca ttattatcta
     5641 aaaaagaaaa ggacataatg gaggtataat attgaaaaaa tattataacc ttttaggtct
     5701 tcatgtagat gaggtaaaac aatattttga tgagcaaaat ataaattata ccgtaaaatc
     5761 tattgaaggt aaaaaggata aagaaaaact tgtagttcct agagttataa aaatatctga
     5821 gataggtgat agtgtagaac tgataatcac atacttttct gactccttag tttaaggagt
     5881 cagaacaata ttggaggcaa aatatgaata acaacattat atatgatata gacttaaata
     5941 acatacctac tcatattgct attataatgg atggaaatgg aagatgggca aaagctagat
     6001 ttcttccaag aactgcagga cataaggctg gagtagaaac tataagagat atagtaaaag
     6061 aatgctcaaa attaggtgtt aagcacttaa cattatatgc attttcaaca gaaaactgga
     6121 aaagaccgaa acttgaagtt gacacactta tgaacttatt atctacatac ttaagaaatg
     6181 aaatagctga acttcaccaa aacaatgtaa aagtaactgc tataggagat ataagtgctt
     6241 taccaaaaac ttgtataaga gaattaaact cagctaagga actgacaaaa aacaatacag
     6301 gagttaactt aaatttagca ttaaactacg gaagtagagc agatataaaa aatgctctta
     6361 tagatatagt aaaaaattgt gaaagtggta aaatagatat aaacaatatt gatgaagata
     6421 ttataaaaaa ctatttaagc acaaagtcta taccagatcc agacttggta ataagaacaa
     6481 gtggtgaaca aagattgagt aactttttac tttgggaagt tgcttattct gagttttact
     6541 ttactgatat acattggcct gattttaaaa aagaagaact acagaaggct atatacgtat
     6601 atcaaagaag ggatagaaga tttggaggac ttaagtaagg agattatttt atgcttacaa
     6661 gaattattgc ttcactagct ctggttccat tatttctatt tgttgtatat ggaggtattc
     6721 ctctatatat agctgagaca gcaatagttt atattgcatt gcatgagttt tataaggcat
     6781 ttaaaataaa agatgtacat ccaatattca ttataggata tttattttct atttatttag
     6841 ctgtaaaaaa tatttttaat ttaccattag aatatactta tgcagtaatt tttattttat
     6901 ttttagcaag tataatttat atgttgatgg gcaaaaacaa tgttatagat gtttcgatta
     6961 cgtttttagg agttttttat ataggagtat ttttagattt tataattata actataaatg
     7021 gatttgaaaa aggtagtata tatgtatggc ttatatttgt gatatctttt atgacagata
     7081 tatttgcata ttttagtggt tatttacttg gaaaacataa gctaattcct aaagttagtc
     7141 ctaaaaagac tatagaagga gctattggag gaataatagg aagtacactt tgttgtattt
     7201 tatttggata tttatttggc attgatttgc ttcaattagc gataatagga agtataggaa
     7261 gtgtaatagc tcaattagga gatttatttg catcttctat aaagagatat gtagggataa
     7321 aggattatgg aaagataata cctggtcatg ggggtatatt agatagattt gatagtgtta
     7381 tattagttgc accttttgta tatagtgcga ttaaattttt tattagataa agattggttg
     7441 gatttaaaag agcaatctat aattgctagt aatgaaaata gctatgtaac ttttaaatgc
     7501 agagatattc tgcagttatt agttacatgg ctattttcat tgttcaaaaa tttagaagac
     7561 cttggggaat tgtataagaa taggtattac tttataatag tatactatat ttgtagtgag
     7621 tatatttttt atactgtaac tatcattaaa aaatattttt ttgtttaata atttcgatat
     7681 ttttagcttt attatatatt tataatatat atgtataaat gta
//
LOCUS       DAMJYX010000129         7722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJYX010000129 DAMJYX010000000
VERSION     DAMJYX010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.134"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            113..1273
                     /locus_tag="PC479_003140"
     CDS             113..1273
                     /locus_tag="PC479_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2805661.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1615..1776
                     /locus_tag="PC479_003141"
     CDS             1615..1776
                     /locus_tag="PC479_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805662.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            1969..2598
                     /locus_tag="PC479_003142"
     CDS             1969..2598
                     /locus_tag="PC479_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2805663.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2837..4096
                     /locus_tag="PC479_003143"
     CDS             2837..4096
                     /locus_tag="PC479_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2805664.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4355..5350
                     /locus_tag="PC479_003144"
     CDS             4355..5350
                     /locus_tag="PC479_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2805665.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5596..6663
                     /locus_tag="PC479_003145"
     CDS             5596..6663
                     /locus_tag="PC479_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2805666.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7197..>7722
                     /locus_tag="PC479_003146"
     CDS             7197..>7722
                     /locus_tag="PC479_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2805667.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITST"
BASE COUNT         3112 a          743 c         1319 g         2548 t
ORIGIN      
        1 attagcatga tatagataaa agtatttatt ttaattgtct attatctgaa aaagtcttta
       61 cttagaaatc ataaaaatca aattaattag gaaaagaaag ggtgtgtata atgtgattga
      121 gactggattt gtatattgta gtttcttggt tgtattcgta ggtgtaattt tttttatggt
      181 tgataaattg aaatggaagg tatttgatgt tattaatcca atgcttttaa tatacttagg
      241 ttcagctata cttggatcat ttggtttatt tgcacagaat gaagatgttg gaacgtatca
      301 aaacataata acagtgaact tccttccatt aatgttagtg tttttgttaa ttcaatgtga
      361 tttaagaaaa atattaaaga tggggcctaa aatgttatta tcatttttct gtgctacatt
      421 tacatttatg cttggatttg ttatagcatt cataattttt aaaaattcaa tgggtgcaga
      481 ttcatggaaa attcttgggg cagcatctgg ttcatggata ggtggctcat caaatatgat
      541 tgcagcagct aatgctctag gagttgcaga agaaggactt agttatgcaa tacttatggg
      601 ttctatagga tatacagtat ggatgtctgt ttgtctaatg tctgtgaaat ttggacctaa
      661 atttaacaaa tggacaaagt gcgatacaag ttttatagat gaggttacat caaaacttga
      721 aaaagaaaat aaagataata cagcacctac gtttgtagaa cttatgattt tactttctgt
      781 tgggtttgga gttgcgtctt ttgcacaaat tgtatccaag atacttccaa cttcaggtat
      841 acttaatgaa actatgtggg tagtacttgt tgcatctaca gtatcaatat tattgggtat
      901 gacaaaaata tcaaagctta aagggagtac actggtagga aatgtgttta tgtatttact
      961 ccttgcagct acaggtagta aagcaaattt ctttggattg tcagaagcac ctgtttacat
     1021 aatgtttgga ttggtagtag tccttgtaca tgctatttca tttatagggc ttgctaaact
     1081 atttaaactt gatttattta cttgtgaagt aggaagtata gctaatatag gaggagtaac
     1141 tacagctcca atgattgctg gggttcacaa tcctgtatta ataccagtag gtgtacttat
     1201 ggggcttcta ggaaatatta tagggactta ttgtgctctt atggtaactc aaatattaca
     1261 tatgttagct taataaaaat atggtataaa attaataatt taaataagaa aagtcatttc
     1321 aatttaagca gaagctttta ctgaaatggc ttttttgttt gacaagataa tctatttcaa
     1381 gaagatgttt tgacatattt agtgtataat attgtaaaat ttactaaaaa cgagatatat
     1441 ttaattttta tattgaatcg aacgtctgta tgatgtataa taaatataca gaacgaatgt
     1501 ttgcagaacg ggtgtttttt gaatttattt taataattta tgaatatata tcagaatgca
     1561 taaatatatt taaatataaa gattaaaaag aatttaaagg agtgttgata acttatgata
     1621 attccatcaa gaagaataca agatataaca ttaacaaatt taaaaaatgg tgaagtaact
     1681 ttaattgaat tggatgaaat atacaagaag atgggatttt tatttgttgt tagtgaaggc
     1741 aaattgaaaa agataaaaaa ggagaatcat cattagaaat gaatgaaata ataaatatta
     1801 taatagttta taaaacctaa cttatgtaga atataaatac ttaagttagg tttttttgtg
     1861 ataaagttta taagtactca attttataag ataaataatg gtcttaagtg aaaagtaaat
     1921 aagtttaaat tttagtaagt attatattgc ataagaggtg atattattat gaaaatagta
     1981 acctacaata ttcataaagg tatggattca aataatagat taactctaac taaaatgggg
     2041 ttatatttaa aacaattgga ttgtgatgta atttgtttgc aagaagtatt atatcctcaa
     2101 tttttagctt taaaagctgt tttaaatatg gatggtgtct ttgcgacaaa tgtaaaaaaa
     2161 gtaaatatga tatatggtat ttgtacattt acaacatcta aaatgttaaa cagtaatcat
     2221 tttttcttaa ctagcaaaaa agaacaacgg ggagctcttt gtataactat agatgcatat
     2281 ggaagaatta taaatgtaat aaatactcac ttgggacttg ataggcagga gagagcaaaa
     2341 caattagacg aaataataga ctataggaac agattagtgg ggatagttat tttatgtggt
     2401 gattttaatg agaaaaatgt ctttttaagt atgtttaatg atatggctat ttcactaaac
     2461 aagtcttact taccaacatt tgaaaaatca aactctagaa tagattacat ttttgttaat
     2521 aaaaatactg aactaaaagg atacacagta gaaaaaattt acctgtcaga ccattatcct
     2581 gtaataggtt acatttaaat ttcaatataa atgaaattag ttgttataaa ataaacaatt
     2641 ttttgttaag aaatagtaaa agaaatataa taaaaaatat caaaaataaa actaattata
     2701 agacataaat ttacaataaa gttacatttg attaaattcc ttgtttaaca aaagctattc
     2761 aaatataatc taaggtagtt ttgaaatggg aactctatta tgcaaattta agctagtgtt
     2821 aaaatgaggt gaagttatgg gcagaagaac aacaagagtt tcaagattag gtaaagaaaa
     2881 aaaacgaagt gttttaattg tattggcagc tttagttcta ataatgggtg gatttactta
     2941 tttttttaac agtaaatttc tgtacaatgg gaaaatcgct aaaaatgtat atatagaagg
     3001 agtaaatgta tctgatatga ctaaagcaga ggcattaaaa gctataacag ataagtatac
     3061 tccagaagat ttaaatcttg cttatgatgg gaaaaaatat acaatatcac caaaagatat
     3121 tgatttaaaa tatgatacag aagatgttgt aaaagatgct tatgaaagta caaaaaaagg
     3181 gtcctatttt caaaacttaa aaaaatatat tgatataaga gtgaataagg caaatatgaa
     3241 gataaaagct gaatatgatg aggcaaaact tagttccaaa gtgtcttcta tagcagatag
     3301 tataaatgtt aagatgaaaa atgcttctat tagtgttgga agtggtggac taagttatac
     3361 agactctgtt gtaggtagag aatttgattt ggcagcaaat aaagagtcta tatataatat
     3421 gataaaaaat aaagagcata aaactttaga acttaaagta aatcttcaaa aaccagatat
     3481 aacaactgaa caagtaaaaa ctgtaaattc tgttataggt caatattcaa ctacttattc
     3541 acaagctgta gagggtaggt catataatgt aggattatca gcaagaaaaa ctagtgatgt
     3601 attattaatg ccaggtgaag aattttcata caataaactt acaggaccta gtaataaagc
     3661 aaatggttac aaagatgcac ctgttatagt ttatggtaag ctagaacaat cagcaggagg
     3721 aggtgtttgt caaacatctt caacagttta taatgcggct cttctttcgg gtatggagat
     3781 aactcaagtt acaaatcatt catctgcatc tacttatgtg cctaaaggga gagatgctac
     3841 tgtgagcgat ggaggattaa acttaaagtt taaaaatcca tataagcatc ctgtatatat
     3901 taaaaattat gcaggtggag gaagtgtatc atcagtaatc tatggtaatt ctggtgacaa
     3961 accaaatata tctatagagg taaaacaaac tggtcagaat aaatattcta catatcgaat
     4021 atttaaagat tcaagtggaa aggtcataaa aaaagaacat atatcaaata gttcttataa
     4081 agaattaaaa aaatagataa ttttttagag aaatatttgt aatataaaag ggagtgtctc
     4141 aaaatgaact ttttagttta tgagacactc tttttctgtg aaatcaaata tattttcatc
     4201 atttcaacaa tacaaaagaa gcgtatctct attttgagac agcctttttt gtaaaaatta
     4261 acactattag ttcttgtttt aatattacaa tcattaatat atagaaaata ttcctatata
     4321 ttggtataat tgtaaataga tttagttata taaaatgtat gaggaggtga catggttgaa
     4381 ttttataggc aatagatatg aggtagtaaa ttctgacgac gttttagaaa ttaacaaaat
     4441 ctataaagct agagatgttt tttacaggaa aaatgtgtta ataaaagtta taaagcacaa
     4501 taattatatc tgtgaagatt ttgtttcaaa tttaattgat gaaagtactg ctttagatga
     4561 gataaactct ccttatatac taaaaataat agatgttggg attcactgta cagaagaaac
     4621 aacattatat tatgttgtaa gtgaagactt taatggtatc ggactagatg aattaattct
     4681 aggtaattat cttcatttag aagcaattgt aaacataatg atacaagttt taaaagcttt
     4741 agaaatgata cataggaatc attcatatca tggtagttta aaatcgagta gtataatagt
     4801 agatacagaa tacaatataa aaatttgtga ttttggaatt acaaaagcaa ataatggggt
     4861 taatacaaga agctatggaa acaggagata tttatgccca catcaactat gtataaatta
     4921 tactgataaa gaaagtgatt tttttgcaac aggtcttatt ctatttgaat ctatatttaa
     4981 aaaattgcct tttggagaat caaattcaga agaaaaaatg ctacaatcta ttgataaggg
     5041 attagattgg aatagtataa aagcaattaa tggaaatact gaacttataa atgttataaa
     5101 aaggcttctt ggaagaaata ataagtatac tagagccaat gatattatta ttgatttaag
     5161 taaagtaatg tatgaaaaag catatataga agttgaagat gagaaagaag aagagcagaa
     5221 acaagacaaa gttatacaag ttaaaaaaga atataaaagt aaaatgagaa aaaaattaca
     5281 taaaaaactt gttgttgccg gtctagctat agtcatgatt tcaatgatta ttattagttc
     5341 cataatataa aaattgtata ttaagtattt taaaatcaat gtctataaag gatatacata
     5401 gaaattttag tgtagtctta aattatgaaa ggaagtggtt attatgttaa caaaaaataa
     5461 tgaggtatta aacgtctagg agtattttaa caaatagcca cctgctttta cagtttagaa
     5521 taaaaaagct gtggctaaag gtcacagctt tttgctactt tttaaaggca ggagaatatt
     5581 aaaaaggagc aaattatgag caatataaaa ataatagcaa gtaataaatg ttggattgaa
     5641 gatatagcaa ggaaacaact tgaagatatt tcaaagttaa atggaatttt aagagtagta
     5701 ggattacctg acttacatgc aggtaaaata cctgtaggtt tagcagttga gactaaaaat
     5761 attatttatc cacatataat tggaaatgat ataggatgcg gaatgacttt atttaaaact
     5821 ggggttctaa aaaagaaatt taaaaaagac aaatggatta agtcacttag taagattaaa
     5881 gatttgtctg atatagaaat aaaaaacaca tataaagaag aatgtccaat tttaaattta
     5941 ggaactatag ggagtggaaa tcattttgca gaaattcaat gtataagtga aatttataat
     6001 aaagaacaat ttgaacaatt aaaattttca tctgatgata ttatgatgtt agttcactgt
     6061 gggtcaagaa attatggaga agatatttta aaaaaatttt atgataaaga tggacttgaa
     6121 gttgaaagtg agaaagctat agattatatt aaaaaacatg ataatgcttt aatgtgggca
     6181 gagcgtaatc gtgaagctat cagtaaaaaa attatgcagt caataggtac atctggagat
     6241 gttgaaacaa tattctctat aaatcataat tttatagaga aaagggaaga taaatttata
     6301 catagaaaag gtgctgtttc aagtgaaaga ggagctgtga ttataccagg ttcaagaggc
     6361 agtctatcat atatagttat gcctactgaa aatacagaga tatctttata ttctctatca
     6421 catggagcag gtagaaaatg gtctcgttca gtttgtaaat ctcgccttaa aagtaagtat
     6481 agtaaagata ctataaaaca aactaaattt aaaagtcaga ttatatgtaa tgatgtaaat
     6541 ttattatttc aagaagctcc agaggcatat aagaatatag agcagattat tgaaagtttg
     6601 atagaatatg aacttattca agtagtggca actatgaagc cattgattac ttataaagga
     6661 taatttttaa agttaaaaaa gattaaaatt atattataaa tagctaattt tatggaacat
     6721 ttaaaagtgt tttattatta gctatttttt tattttgtag agacgataat ttatagagct
     6781 aaattctttg atatataatg attacaaagt tagactgtat tggaaactgc acatacatga
     6841 attgatgaca atataagtga ttcccaaata taaatgaaag tataagctta tttttactat
     6901 aaaaattttc actgtacaaa aacattaatg acattatttt tattgacata aatatataat
     6961 ttatggaata atattagaag cactgatata agtgtttaaa taagaataga aaaggagaag
     7021 tgttttatga aaaaaaatac taacattaat gagtccggag ttaaacttct taataaataa
     7081 ggagtttgat atataataac tccttattta ttaagttgtt cgcaaaataa ctattatcaa
     7141 agtaaatgat agggtgattt aaaagacgat gacttataaa taaaggagaa aaaataatga
     7201 atagtaatac atttgaaaga ttacaactaa atgaagttaa agacttaata aaaatacatt
     7261 gtgtaagttc gctaggaaaa aatttaatag ataaattaac tcctagtggg aatattggag
     7321 tagtaagaag gaaattagca gaaaataaag aagctagaaa gataattgaa aatagtaatc
     7381 acataccact tgaaggattg tttaatgtag gttctacaat agataaaatt gaaaaaggaa
     7441 tgataataga acctgttgaa cttgtaaata ttgaagattt tttaagaggt tgtaggaaga
     7501 tgaaagcttt tatgttagaa aaagaatttt attcacctac attaagttct tatgccttaa
     7561 atataacaga atgtaaaagt atagaagatg agataaatta ctgtataaaa tctaataaag
     7621 ttgattctaa tgctagtaaa gaattaaaaa agataagacg aaatatagaa attacagaag
     7681 gaaagataaa agatagatta aataaattta taacatcaac tg
//
LOCUS       DAMJYX010000130         7715 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJYX010000130 DAMJYX010000000
VERSION     DAMJYX010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.135"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            322..1623
                     /locus_tag="PC479_003147"
     CDS             322..1623
                     /locus_tag="PC479_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2805668.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1699..1974
                     /locus_tag="PC479_003148"
     CDS             1699..1974
                     /locus_tag="PC479_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2805669.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3309..3539
                     /locus_tag="PC479_003149"
     CDS             3309..3539
                     /locus_tag="PC479_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2805670.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3532..4113
                     /locus_tag="PC479_003150"
     CDS             3532..4113
                     /locus_tag="PC479_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2805671.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4692..5369)
                     /locus_tag="PC479_003151"
     CDS             complement(4692..5369)
                     /locus_tag="PC479_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2805672.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5558..6922)
                     /locus_tag="PC479_003152"
     CDS             complement(5558..6922)
                     /locus_tag="PC479_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2805673.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7050..7571
                     /locus_tag="PC479_003153"
     CDS             7050..7571
                     /locus_tag="PC479_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2805674.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3015 a          880 c         1328 g         2492 t
ORIGIN      
        1 gagtaatgaa ttatattgaa cgatatatca ataattggca gtaagttata tcaaataaga
       61 tattactatc gagtaatgaa ttatattgaa caatatatta ataattggca gtaagttata
      121 tcaaataaga tattgctatt atgtagtaaa ttatataaaa caatgtatca gaataaatag
      181 tatattaata atttgtagta tatcatgacg aataatacag tcataacaaa tagtgagtca
      241 taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata aaaacttata
      301 aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga gaagtctatt
      361 ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt aaatgaaaac
      421 atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact taaaaaagaa
      481 aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc tctagcaata
      541 aaaacaagtg atgtgactaa tctaattact tggggtcaga attttggtct tgatggtcat
      601 attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg ccaaaacctt
      661 ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt tgaaagtaat
      721 ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag tatagcaggg
      781 aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg tataaaatta
      841 cttgatacca ctataggagg tctttttaaa ggagagctaa ccacaatagc agctaaatcg
      901 ggggttggga agactgcttt ggcacttcaa ataatgttaa attcatttaa gcaaggtaaa
      961 aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag aaatatatgc
     1021 agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga aaatgattgg
     1081 aaacttattg taaatgcgat aggtgattta agcgaaaata acttaattta tatcaatgat
     1141 aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc agatttatta
     1201 atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag agaaagggag
     1261 gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat acctgtaatc
     1321 caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga tagacctatg
     1381 agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca tcaattaaag
     1441 gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc agttagagca
     1501 tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag agatggacag
     1561 actaggcata agcatttttg ttactttggg gataagttac attttcaaga actaaattat
     1621 taggtcaata aagtagtaat aacaagctta ttaaaagtcg ggaaatagca aatagattat
     1681 aaaatatgag gtgaaaatat gttggaatta agaaaaatta tagaattact tgaagaacac
     1741 aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga ataccttgaa
     1801 ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg ggctttaaaa
     1861 gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat ggaattagcg
     1921 aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa atagatattt
     1981 ttggagaata caagtgtgat atacttctga aatagaagtt attataagag gagtataagt
     2041 gtgattaaca acatgatttt gtgaataaaa ttgaggatat atatgagtta aatgtgtgat
     2101 taataaggta agattgtgta taagattatg agtatttaaa aggaggggct ataattaata
     2161 gtgtagagtt gtgtgtttgc ttaagattat ataataattt aatagattaa actatactaa
     2221 taaggtaaaa tcgtgtgttt agttaaagct tattataaaa tcttgatatg tgattaatga
     2281 tgcaatatgt gaataaagtt aagattataa tagatttaac ctgtggctaa tagaataaaa
     2341 ttgtgaataa agttaagatt ataataggtt taaattgtga ctaatagagt aagagtgtaa
     2401 atagagataa acttcaatat gagcttaacc tatgattaat agagtaagaa tgtaaataga
     2461 gataaacttc aatatgagtt taacttgtgg ctaatagagt aagagtgtaa atagagataa
     2521 acttcaatat gggtttaact tgtggttaat agagtaaggt gtaactaaag ataaacttca
     2581 atatgagctt aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg
     2641 agtttaactt gtggctaata gagtaagagt gtaactaaag ataaacttca atatgggttt
     2701 aacttgtggt taatagagta agaatgtaaa tagagataaa cttcaatatg agcttaactt
     2761 gtgattaata gagtaagagt gtaaatagag ataaacttca atatgggatt aacttgtgat
     2821 taatagagta agagtgtaac taaagataaa cttcaatatg agcttaacct atgattaata
     2881 gagtaagaat gtaaatagag ataaacttca atatgggttt aacctgtggt taatagagta
     2941 gggtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     3001 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3061 taaaattata gagctgcata agaaaatatt atagatatat taaatattag ataataaata
     3121 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3181 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3241 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3301 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3361 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3421 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3481 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3541 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttgat attgggattt
     3601 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3661 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3721 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3781 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgccaa tatgcttttt
     3841 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3901 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3961 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     4021 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4081 atgatatgta aggcaaagag taagtctgta tagaaaggcc ttatgtgata tttacagttt
     4141 tgttcaagta gagatgagta gtaggtttga atatatatga caaatatatg agtatagggg
     4201 catagattta gtttgtgtaa ggtaaactga tttatgcttc ttttttatgt cttagattat
     4261 tggctaagtg gaaacaatga atttagatat taagaattat tcagttattt gaagaaaata
     4321 gaataataat taacattata ttaatggata ttcattacat ataagccagt taatagcaag
     4381 atttatttaa tatgatatag gtcaagtatt taaactaaat aaggtcatga caattaatat
     4441 aagtatagat ataaaaagat tagaagttat aacttataaa aggtattcta tataaatagt
     4501 atggggaaaa tagtcaaatt aacttattag aaacaaaaaa tatacataaa aaataaatgt
     4561 aaatatcaaa taagatacta tatcaaaata gatataagta tcttttttat tattgaaatt
     4621 atgaaaatat atttgatttt acacaaaaaa gagtgcctca tgaactaaaa agttcatttt
     4681 gagacacact tttacagtgt caaagaacga agtttatcct tatccaatac ctttatacca
     4741 ccacgagata aagacacaat accttctcta gcaaaatatt tcaacattct cgatacaact
     4801 tctctggcac tacccatata cttagcaatt tgctcatgag tcatatttat agtgctactg
     4861 ccatttttag ctatctcatc aattaaaaaa gatgccaaac gcttatcaaa actcataaaa
     4921 agaatttgtt gcatagccca cataacatca gaaaaacgtt cagtagctaa tttatatgaa
     4981 aataactctg catgaatatt ttctgtagat aattttgcaa aaacagagga actaatctgt
     5041 ataatatcgc agtccgtttc agcatctaca tagacatcaa aagttattgt ttttagaata
     5101 caggaagctg ataaaataca tacatctcca tcatcaagtc ggaacagtgt aacctctcga
     5161 ccttcatctg aaagtatgta tgtacgcact gttccagatt ttataatcaa aataccaata
     5221 cagtcactat caccacaatg tactggattc ccttttttgt aatgcgaggt atttgctgaa
     5281 gaaattaata attctttttg taagtcagta agtttatccc aaaaaggcaa gctatgtgta
     5341 agtatatcaa tttcattttg tgaaatcata caatatctcc tttcttaaat tgttataatc
     5401 ccatgataca agtgaataga taaattgaca agtagtttgt taagaggatt atttagtcag
     5461 atagtaatag tatgatttat gtagaaaaaa tatgataata tatattcttg ctattaatga
     5521 gaaagtaata tgttttgatg atagtaggtt tgtataacta atgcataccg tttattttta
     5581 aaagattaag ttccttatgt aaccttatag acataaaacc agcaattata gcagccatca
     5641 aatcagctat tggttgtgca tataagatac catttaaccc atagaataca ggaataatca
     5701 aaattatagg gataaaacag acaccttgtc tacacatacc taaaaatcca ccttcctttg
     5761 cttttcctag tgcgaggaat aatgtagaat acactgtata gaatccaaat agcataaatg
     5821 aaaacccatt tgccctaaga gctttactac cattttctat taataaaatg tcaccacttg
     5881 taaattgtga aattatagat tttgggaaga gtatcaatat aagaccaagc actacacaaa
     5941 atactgtagt ccataatatg gaagttttta ttgctatatg taatctatcg tagttctttg
     6001 aaccaaaatt ataaccagca attggttgga aaccttttat aaaaccaaat acaacaagac
     6061 tacctaaaga cgttattcgt gttacagcac ccattccagc tatcacagaa tcaccatact
     6121 ttgtagcctc catattagtt aatgtaatag caagactagt caataattga aatattaagg
     6181 taggtatgcc aatttttagg atttctgaaa aaatttcttt tgaaaaacag cattcgttaa
     6241 ttctaaaact aaatatactt ttcttgctta aaatatatcc tacataaaca agtgttgaag
     6301 caatctgtga gatagctgtt gcaatagaag caccccttac ccccagacca aaagtataaa
     6361 tgaatatcgg gtctaaaatc atattaagca aagcacctaa taacatagca cacatagttg
     6421 ttttagcagc tccttcactt gtgacaatat tagtcatagt gacattgaat atattaaaaa
     6481 tagatgacac gacataaatg ctagcataag atattgcata aggcataata ctttttgtag
     6541 ctcctaacaa ttttaaaatt ggattgagaa aaatcataat cacaataata actaatgtac
     6601 ccactataat actactgtaa atagatgtac ttaccacttt tcctgctttt tcattatcac
     6661 catggcctaa aagtctggca acatatgaag cagctccatt tccaaacaat aatcctaatc
     6721 ccacaacaag ctgacctaat ggaaatgcaa cggatatagc tcccatttga cttgttccaa
     6781 gtccacccac aaagtatgca tcaaccaaat tgtataatgc agttatcatc atacctatca
     6841 tggtaggtaa tcccatagat aacaatgctt ttgagattgg ggcagaccct aataattcaa
     6901 ttcttttgtt actttctttc atacttaaca ctcctcttct attgacatta agtctaaatt
     6961 ataatactat aaattatagt aagtatggaa aatagtacta taatttatag tatttgtcaa
     7021 tagtaagaaa actaaaggag atgattttta tggctacaat agatttaatt gtattgggaa
     7081 tagtaaagca acaacccaag agtgcttatg atattcaaaa ggcagtggaa tacaggaata
     7141 tatcacgttg ggttaaaata agtacaccat caatttataa aaaagtaatt caattagaac
     7201 aaaagggata tattgaaagt aaaactgtga agaatggaaa aatgccagaa aaagcagttt
     7261 attcattaac tgaattaggt gagacacatt ttttagattt aatgtttgat acatctagca
     7321 aaccaataaa tattctttta gattttaatg ctgttatagt aaatttaaat agccttacac
     7381 cagaaaaaca gaaattatgt ctcaataata ttgaagagaa tgttcaagag ttaaagaaaa
     7441 ctattgagga aaatataagt ataaagacag aagtaccaga cacagcaaaa gcagtgttgg
     7501 agcaacagtt ggtgctagca caagcattgg aaacctggat aaattcttta aaagataata
     7561 tgggaaatta gaattagaga agctaactta ataatcatta aacaaaaacc agcatattta
     7621 ataatgagat attagaaaaa ataaaacaaa tgatataaac tgaaaagtat atttaaaata
     7681 aagctcactt aattgaggga gaatgttaaa ctttt
//
LOCUS       DAMJYX010000131         7695 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJYX010000131 DAMJYX010000000
VERSION     DAMJYX010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.136"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            43..354
                     /locus_tag="PC479_003154"
     CDS             43..354
                     /locus_tag="PC479_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2805675.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      447..539
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            639..1433
                     /gene="thiD"
                     /locus_tag="PC479_003155"
     CDS             639..1433
                     /gene="thiD"
                     /locus_tag="PC479_003155"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2805676.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            1457..2245
                     /gene="thiM"
                     /locus_tag="PC479_003156"
     CDS             1457..2245
                     /gene="thiM"
                     /locus_tag="PC479_003156"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2805677.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            2268..2900
                     /gene="thiE"
                     /locus_tag="PC479_003157"
     CDS             2268..2900
                     /gene="thiE"
                     /locus_tag="PC479_003157"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2805678.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            3039..3341
                     /locus_tag="PC479_003158"
     CDS             3039..3341
                     /locus_tag="PC479_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2805679.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(3407..4162)
                     /locus_tag="PC479_003159"
     CDS             complement(3407..4162)
                     /locus_tag="PC479_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805680.1"
                     /translation="MGMLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMN
                     NININNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQL
                     LEAILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLP
                     NEIVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETI
                     LIGDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(4150..5112)
                     /locus_tag="PC479_003160"
     CDS             complement(4150..5112)
                     /locus_tag="PC479_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805681.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(5116..5574)
                     /locus_tag="PC479_003161"
     CDS             complement(5116..5574)
                     /locus_tag="PC479_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2805682.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            5930..6286
                     /locus_tag="PC479_003162"
     CDS             5930..6286
                     /locus_tag="PC479_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805683.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            6358..7074
                     /locus_tag="PC479_003163"
     CDS             6358..7074
                     /locus_tag="PC479_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805684.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            7067..>7695
                     /locus_tag="PC479_003164"
     CDS             7067..>7695
                     /locus_tag="PC479_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805685.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVI"
BASE COUNT         2991 a          893 c         1258 g         2553 t
ORIGIN      
        1 taatataata tttatatatt taatgttagg atgtgattta aggtgtattt tacatatatt
       61 ataagatgta aagatgattc tctatacact ggatatacca gtaatatagt tagaagaatg
      121 aatgaacata aattaggaat taattcaaaa tacacaagag caaagggatt tgaaaaacta
      181 gaagtttatt ttgtgaccaa cacaaaaagc aatgctatga agttagaata ctatataaaa
      241 aaattaacta gaaataaaaa attgtctata ataaaaaacc caagtatact tataaattta
      301 attgataata aagaagatta tattattggt aaggaaatag aacaattaac ataaatattt
      361 atagtaacat tataatgggt gcatatggtg taaaaacttg acaataaata ctattgtaat
      421 ataatgaagt tataaatata gtaaattaaa atagctaggg gagccaagag ctgagaggta
      481 taccgaccct tacacctgat ctggataata ccagcgtagg aaagcttagt aaaaattcaa
      541 gtatgtatta tttgtgatat atgctatttc taagctatac ctattgtagg tatagctttt
      601 ttaattaagt tatttaaatt aaactttagg aggcaacaat gagtaattat aaaataccaa
      661 cacttacaat agccggctct gattcatctg gaggagctgg gattcaggct gatttgaaaa
      721 cgtttagtgc aattggtact tatggtatga gtgtaataac agctataaca gcacagaata
      781 ctaaaggagt ttttgctgta gaggatttaa ataagaaaat aataaaaaaa caaatagagg
      841 cagtctttga agatatacct ccaagagctg taaaaatagg aatggtttca agtcctgaga
      901 ttatacttga aatagttgag aatctaaaga aatacaatcc aaaatatttg gttgtggacc
      961 cagtaatgat atctaaaagt ggatattatc tattaaagcc agaggcaaaa gaaaacttaa
     1021 ttaagtattt gataccactt gcatacataa ttacaccaaa tataccagag gcagaagaaa
     1081 taacaggaat aaaaatacat aatgtagatg atatgaagag agtaggagaa gaaatactac
     1141 aacttggacc taaatttgta cttatgaaag gaggtcattt agatggagaa gcagtggata
     1201 ttttagttgg taaaaatata tttaaagttt ataaaagtga aagaattgat aaaaaaaata
     1261 cacatggaac agggtgcaca ttatcttctg caataacatc ttatctagca ttaggatatg
     1321 aaataacaga agcagtaaat ctaagtaaaa tctatataac agaagcaata aaaagaagtt
     1381 ttgatatagg tcatggagta ggaccagtac atcattttta taaatttgag taataaaaaa
     1441 atattggagg tagaatatgt ataatttaat taaagatgta aaaaaattaa atccattggt
     1501 aattcattat actaataatg taactataaa tgattgtgct aatgtaactt tagctgtagg
     1561 agcaagtcct cttatgagtt tttcatatga ggaagtagaa gaaatggtaa gtgtagcaaa
     1621 ctctgtagta ataaatatag gaactatgaa ctcaaatatg ttggatttat tcctattagc
     1681 tggaaaagca gcaaataagt acaataaacc tgttgttttg gacccagttg gagtatttgc
     1741 aagcaaagcg agagcagaac ttacaagtag acttttaaat gaagtcaaat ttagtgttgt
     1801 taaagggaat gtatcagaaa taaagtttat aggtggcttt aatgtaaggg gtaaaggtgt
     1861 agattcattt gatgaagaag aagattctac tgagataata agaaagattg ctgaaaagct
     1921 tgaatgtgta gttgtagcaa ctggaaaaat agatataata actaatggta aaggtacata
     1981 taaaataaac aatggaactg ataaattaaa aggaattaca ggaactggat gtatgactgc
     2041 tagcttgatt gcaagtttta tggctgttac tgagaatata ttagaagcag caacaatggg
     2101 tgttcttaca atgtctttaa gtggggaatt agctaattta aataatccac caataggtac
     2161 atttaaagaa aatcttatga atgctattta tcaaatggat atagatgctt taagtaaaaa
     2221 ttctaatatt gaatttttaa attaataaag agtaagggag acttaaaatg attgataaag
     2281 aatctttaaa aaaatgttta aagttgtatt tagtaactga ctctgaaatg cttaaaggta
     2341 gagactttta taaatgtctt gaagatgcaa tatcttctgg aataacaact gtacaactta
     2401 gagaaaaaaa tgcttctgga agagagtttt taagaaaagc tatgaaactt agagaaataa
     2461 ctaaaagata tggtgttaag tttataataa atgatagagt ggatatagca cttatttgtg
     2521 atgcagatgg agtacatgta ggacagagtg acattgatgt tagagaggtt agaaagctta
     2581 taggaaacaa taaaattcta ggagtatcag caagaacttt agaagaagct atttgtgcaa
     2641 aaaatgatgg cgcagactat ttaggtgtag ggtctatatt cacaacatct actaaattag
     2701 atgcaaaatc agcttcgttt gaaactgtta aagaaattaa agaaaaagta gacatgccat
     2761 ttgtactaat tggaggtata aatttagaca atatagataa acttaaatgt ttagaaagtg
     2821 acgggtatgc tataatatca gctatattaa aagctgaaga tatctctaaa gaagtggaaa
     2881 agtggacatt aaaaatataa aattggctct ggtagtgaaa aaaatggaaa aatttgatat
     2941 aatctaagag taaaatttaa gcaattataa atgataaact tttctacata tactttttaa
     3001 tcaagatata tgtagatttg gttaaaaagg agctataaat gattaggata ttgttagtgt
     3061 gtgttggagg catgtcttct accctattgg taaataaaat ggagaaggat gcaaaaaaaa
     3121 gaaatataga ctgtaaaata tgggcagttg gagaaggaga tataaaatcg gagcttgata
     3181 actttgatat tttacttctt ggaccacaac ttagatttat gcttgatgac gtaaagtcta
     3241 tagttggaga tagagctcca gtatctataa tagatatggt aaactatggc acttgtaatg
     3301 gtcatgctgt gttaaattcg gttttagaaa tattaaaata aataataaaa gtggattcta
     3361 tataatagag tccattttta ttatttgtta ttaataggct taaaacttag cttaccattt
     3421 ctttttttaa tctccacata gcaagtgaaa aacttgaaca gcacaatatt aaaaatagta
     3481 ggataacagg cattatatct ccaataagaa tagtctcacc aaatattaag ctacgtaaag
     3541 cgttaataac atgggtaaat ggattgagca tcactgctac ttttaatcct ccagataaac
     3601 tttctatagg gaaaagagct gaacttaaaa agaaaatagg aagtacaatt gcagtcataa
     3661 ttgtttcata aactatttca tttggcaata ataaacttat agaataagct agacttgata
     3721 aaaagaaggc tgtcataaat atcagtacta tcataagtag gattccacca atgccagatt
     3781 ctatccttac tgagaagaat atacttacta tacacaaaat agttacttct atgaaagaaa
     3841 ctaagatagc ttctaataat tgaccaagga cgattgaata cctgctgata ggtgcaatta
     3901 agactctata gaaacttcca ctgttcttca ttataaagtt tataatacca ccactactac
     3961 aagaagaaaa cacaaccagt acaatgatac ctggtagtat aaaagcagta taattattga
     4021 tatttatatt attcatagtt tggtttgcaa tagaactata aagcacaagc caaagaaaag
     4081 gttgtaatat agtaactaca aatgagattg gatttttaaa acgccacttc atactacgcc
     4141 ataagatatt taacattccc attcctcctt taagcttttt ttagaacttg taagtgctaa
     4201 aaatacatct tcgagacttg gctctacaat ttctattgca tcaaattcta tatcatgttc
     4261 taaaagccat ttatttatta aagtaaaagc agttctacta tcgtttacac ttataaaaat
     4321 tgaattttct cgtacgctca taaactttac taaaccagta cttttaattg aatctttata
     4381 tttttttata tcttcagtat tatgaaaagt tatacgtagt atattttgtc taatgtaact
     4441 acgtaaactt gatggtgttc cttgtgctaa atcctttcca ttcttcataa tacaaatatt
     4501 atcactcaat tgttcagctt cttcaagata atgagtagtt agaaatatag ttgtgccata
     4561 ctcatctcta atttttaata acatatccca catagatttt cttgaatcga catccatacc
     4621 aacagttggt tcatctaaaa ataggatttt aggagatgat accatattca tagctatgtc
     4681 caatctacgt tttacacccc ctgaatatga tgaagtagga tattttaagt aactagataa
     4741 gtcaaactta tcaattaaag agtctattct ttgttttgca atctgtggtt ctactttata
     4801 cattttactt tgaaatacca tattttccat aagggataaa tgttcatcaa tagagatttg
     4861 ctgtgctaca caagcaattt gagtacgtat ccaagaagga ttatctacta aatcttttcc
     4921 aaacattgtt acatttccag atgtaggttt gtaaaaggta gtcaaaatat ttataagaga
     4981 agatttacca gcaccattag gaccaagtaa agaaaaaatt tctccagcat ttacattaaa
     5041 gcttagacca tttaaagctt tcacaccatt tttatattct tttactaaat tgtctatttc
     5101 aatcgctaac atatattatt cctcctcttt aagtggaaat attatctctg taatatagtt
     5161 atctgggttt ccttttatca acatacctgg tcctttaata aatacttctc tccaaggagt
     5221 ttgtattttt atattatttt catgaatata tttaaatatc atttcatacg tttttggcaa
     5281 attactataa gagcctgtgt gagtaaaaaa taaagctttt gttttaggaa attcttttgt
     5341 agttataccc atacgatttg gagtttcagc agttggaaca cataattcca tttctgcaac
     5401 accagttttt tggtcaacat catagtagca aaagaatggt gcaccgttag actttccttt
     5461 tatagcttta aatacatttg gaaaatactt tgttgcctca gtcataggtc cattataacg
     5521 catggtagcc actgtgactg attcgatatt tttgatttca attttgcaat tcatttgttt
     5581 acctcctaat gtgtgttcat attcttacta gatttttaag cattaataca tataagattt
     5641 tgattagttg aaaacatata atcttatctc gtttgcttgt tttcaagtct aacataacat
     5701 aaaatatata tcctctcatt tgatgctcca ttttgtaaag ttattattta agctttactt
     5761 tagatttttt agcaaattaa aataaagcaa tttataaaat aagtatttac aaattaagtg
     5821 tattaatcgt ataatacact taataggata ctagataaag tctgaaataa aggcgtattt
     5881 ttttaaagta agtgtactat cgtattaata cactagaata ggaggaatca tgaattttga
     5941 attagataat acaactccca tttatttgca aatagtaaag tatataaaga gacaaatagt
     6001 aacaggagag ttgaaaccag gagagacaat accatcaaga agagaaatgg cacttaatct
     6061 aaaagtaaac ttaaatacag ttcaaagagc atataaggag atgggtgata tgaatataat
     6121 aaatactttt aaaaattatc aaagcagtgt aacagttgat gaaaatatat taaaaaattt
     6181 aaaattagag cttataaatg aatcactatc tgtttttata gaagatatga aagcaataaa
     6241 tgttagcaaa gaagaagttt taaaaataat agaagataag tattagtttt ttaacaaata
     6301 gaaaacttag tttagaaagt ttatttataa gcatattgga tgtggaggat agtaaatatg
     6361 atagaaataa agaatgtcag caaaacatat aaaaggatgc aagggcttaa aataagaaag
     6421 atagaagctt taaaaaatgt aagttttaat atagaaaagg gaaaaataac tgcattactt
     6481 ggtataaatg gtgttggtaa atcaactatg ttaaaagcaa tagcaggact tataaacatt
     6541 gatagtggag aaattcgtat agatggagaa aaaataaatg aaaaggtata caataaattg
     6601 gcttttgttc cagatgtaca atctcacttt agcaatacaa caataaaaga aacatttgaa
     6661 tttatggaaa ttttttattc aaagtggaat aaagaaaaat caaaagaaat gatggacata
     6721 tttaaattgg atgaagatga aataattgat aacttgtcaa aaggaaacat tgcaagagta
     6781 aaattaatac ttggattttg ccaagaccca gagtacatac ttcttgatga accatttaca
     6841 ggaattgatt tgtttaagag ggaagagttt ataggagtaa tagcgcaata tatggaggaa
     6901 aatcaagcca ttattataac tactcatgag atagtagaga tagaaagttt agttgatgag
     6961 gttgtaattc ttgatgaagg gcagattata acttcattta atgcagaaga attaagagaa
     7021 agagaaggta aatctatatt agataagatg agggaggtat ataagaatga ataaacttct
     7081 atatctaata aatgtagatt tgagaagaag caataagttt tatatagctt atatatcatt
     7141 tttttcatta attacactta gtttaaattt attcgaaatt aataaattta aaaattctaa
     7201 atttattatg aacttagcaa tagaagattt tggaggtata ttttatggac tagggatact
     7261 tagtaatctt ggttttatcc aaagtatatt gttctttggg atattgggac tttttatata
     7321 ctatattttt atgtggaaaa gagagttctt ttcaaataat caaagtattt atacacttat
     7381 gatgttacct caaaataagt ttaagatttg tatatctaaa tcaatagcct tgataacaat
     7441 gatatatggt tttttgataa gtcagacagc aacattattt atatgtaaat atatatttaa
     7501 tttcattttt agaaatatgc ctataatcaa tatgaacttt gccaaagatt tatattattt
     7561 aaacttgaag agtataccaa ttgattttat ctcttttatg gctatttatg tatttttact
     7621 acttatagct atttctattg taaattgctg tatattattt atcttttcct atataaataa
     7681 attttatgta atttt
//
LOCUS       DAMJYX010000132         7690 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJYX010000132 DAMJYX010000000
VERSION     DAMJYX010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.137"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            971..2329
                     /locus_tag="PC479_003165"
     CDS             971..2329
                     /locus_tag="PC479_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2805686.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2436..3230
                     /locus_tag="PC479_003166"
     CDS             2436..3230
                     /locus_tag="PC479_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2805687.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3223..5529
                     /locus_tag="PC479_003167"
     CDS             3223..5529
                     /locus_tag="PC479_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2805688.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5668..6945
                     /locus_tag="PC479_003168"
     CDS             5668..6945
                     /locus_tag="PC479_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2805689.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7128..>7690
                     /gene="yqeC"
                     /locus_tag="PC479_003169"
     CDS             7128..>7690
                     /gene="yqeC"
                     /locus_tag="PC479_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2805690.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGL"
BASE COUNT         3013 a          840 c         1438 g         2399 t
ORIGIN      
        1 tctataaata gatagaaact tgtatatttt atgttgcaga tacaaatata gtaaaggaga
       61 gtacattggt acaactgaga tacatagctt attaagtttt tctgtcttat gagaattata
      121 ttataaaata ttcataaata ttttgagttt aaattaaata attattttat gcttagatta
      181 attgatattt aattaattta ataacaaaaa cagtgaaaga gtaaataata ttatttacag
      241 ggggtaaagc aaagtatgtt aaataaattg tacttttggc cccgtacata gagaaatcta
      301 tgtaggacac aactttaaaa ccagtaaatc ctaaagcctt gcaactacaa taaaagatag
      361 aaagaaactt ttatgaatgt ggtgaaaacc agaaacaata gtgaggatgg tatatggtga
      421 gagcctaagt acttaaaaat ggacatttgg tcgccaagcc tagaaatagg aaggtcaaac
      481 gactatctcg agagagagta cactataagc gattgatagt ggaaacgggt tgctcttagc
      541 aggtaatgct gaagatgaag aaatagtcta cgctcatatg aaagtatgag gtcactggat
      601 ttgaagtgac atctataaag tagcgtttat aggtaaatgt ttaaaacttg aaacatgtaa
      661 atacttatga aaacttataa cttataatga ttaagattga aagatttaaa ttgaattaaa
      721 tttactataa aaaattaaaa attcatagct ataatactct tatatttgta aagcatggta
      781 tataatgtat acaaaattaa agaatacgtg tatttgttaa taattaaatt tattgcatct
      841 gttttttaag actaaaaact taaatatatg ttggagtata gttgatattt atgaacatca
      901 ttaatttaat atattagatt tttaagtatt tatataattc aataagtttt atgtaacggg
      961 agtttttatt atgaaagcaa aaagtacatc aaaatttgaa ttagacggaa taccaccact
     1021 aaaggaagca atacctttag ggttacaaca cttaatggca atggtaatag gtagttgttt
     1081 accagcgata ttgattgcaa atgcagctgg acttaatcat aagatgtcaa cattattgat
     1141 tcaagccgca ttagtttttg caggattatc aactttatta caattatatc caataccttt
     1201 gtttaaaggt tttaaattag gaggaggaat accagttata atgggagcta cagctatgtt
     1261 tataggagct ggggtatctg ttgcaagtaa gtatggacta ccagtattat ttggctctca
     1321 aataatagct ggtatagtaa taattataat tggttttggt cttaaaaaaa tacgatttat
     1381 atttactcca gctgtatcag gaacaatagt tgcatgtatg ggtcttggac tttttccaat
     1441 agctataaaa aacttagctg ggggtatggg caatgagact tttgggaatc caattaattt
     1501 tatagttgga atatctgtat ctcttatgat attaggatta aataagtatg gaaaaggatt
     1561 atttaaagat gcatcaatat tagttagcat aatttttgga tacattttat cattaatttt
     1621 gggtatagta aatttttctt caattcagga gtttactttg gttgcacttc caaaaccact
     1681 tgcttttggg ctagatataa gacttgaagt tgttgtaatg ttttcaataa tatatttagt
     1741 tgagatagct gatataatgg gtgcttgtac actttctgcg gtaggagggc ttaatagaca
     1801 agtaacagat gaagaacttt cttctgctgt tttaggaaat ggagtactct ctacaatagg
     1861 tgcattattt tctgcaactc caatggggat gtttggacaa aattctgcaa tcgtttccaa
     1921 tacaaaggtt gtaagtaaat ttgtactagc tattggagga attggattat ttttggcagg
     1981 aatatcgcct ttattagcta atttaataag aacaattcct ccctgtgtgg ttggaggagc
     2041 aacactcgtt atattttcaa ctcttacaac atctggttta agacttgtta gtatggatgg
     2101 atttaatcaa gaaaatagca tgatattagg tctttctatg gcttctggta ttggattcat
     2161 ggtagctcct caagttcttg aaaaatttcc aaaatttata gagacattac ttgcagattc
     2221 aagtgtagtt tcaggagcaa tggtagccat aataattcaa gctttatata tggtaaaatt
     2281 tcctggaaat aaaagtgata aggttgagga taaaaataaa agtttataat gtaaaggttt
     2341 aatatatgtg aattaaatat actacaatta taaatataaa taaaagattt attaaaaact
     2401 tttgtgtttt aataagttaa tgatgggggg ataagatggt tactataaag gaatattcag
     2461 ttccacagag tttagaagaa gcatataaaa ttttaatttc taaaaaaaat aatgttattt
     2521 taggtggatg tggatttata aaattaagta ataaaaatat aggaacagcg atagatttaa
     2581 aagatataaa tctaaattat ataaaagagg ataaaaaaaa tattttgata ggtgcagata
     2641 cttcacttag aagtttagaa attgataaaa ctataaaaaa ttactgtagt ggaatacttt
     2701 caagcgctgt ttctaatata gttggtgttc aatttagaca aggggctaga attggtgcaa
     2761 gtgtattttc aaagtatggc ttttctgact taataccagc acttttggtt gttggagcga
     2821 aggtgagatt atacaataaa ggaatactag agttaagtga gtttttggat agtgaattaa
     2881 gtagggatat attggtcgaa gtaatactac caaaaaaaga tgcaatagct gtttttgact
     2941 caataagaaa atgcacagga gatttttctg tattaaattc agcaatgcta aaagaaaatg
     3001 atacttacaa aatagcaatt ggggcaagac ctcaaaaagc tagattagca cttgaagcga
     3061 gtaatatatt aaataaagaa aaagatatag ataaggcatc tattgttgct agtaaggaac
     3121 ttacttatgg aagtaatatg agagctagta aagagtacag aaaggatatg gcaagtgcat
     3181 tagttaaaag gatgtataat gctattgagg agggaatgta taatgattaa taaggggata
     3241 agaaagatag atagtacagc aattgtcaca ggaaaacctc tatatactga agacctcatt
     3301 atgcataaag atgttttgac attaaaacta cttagaagtc cacatgctca tgctaagata
     3361 aagaatatag atacatcaaa agccgaaaaa attccagggg ttgtgggtat ttatacgtat
     3421 aaagatgtac cacaggtcag atattgtgtt ccaggtcaaa cagaaccaga agcatcacct
     3481 tatgatagag ttttacttga agatacagtt agatttattg gagatgaagt agctttagtg
     3541 gctgctgaag atgagaaaac agcacttaaa gccttaaaaa tgataaaggt agattatgag
     3601 gttttaaagc ctgtgtttga tgcaagagaa gcagaaggaa atgaagttgt tatacatcca
     3661 gaaaaagaca tagtagagcc ataccctttt ggattggatg caaaaaataa tatagtatca
     3721 aaagaatttt ttgaatttgg agatgttgaa gctaagctta aagaaagtga ttttgtggtt
     3781 gaatcttcat acaaaaccca aagtcaatcg cattgcatga tggaaactca taggtcatat
     3841 tcatataaag atataaatga tagaattgtt gtagtttcat cattacaatc accacacaat
     3901 gtaaagagaa tacttagaaa agtattgggt ataccgtctt caaaaataag agtaattaaa
     3961 cctagaatag gtggtggatt tggaggaaag aatatagcag caacagatat atttactgca
     4021 tttgttacta tgaaaactgg tagaccagca aaatttgtat ttacaagaag tgaaactttt
     4081 gtaggttcta atagtagaca tgaaatgttt tttgatgtta aagttggtgc agataaagaa
     4141 ggaaatttaa aagcaataga gctaaagtca ttaaacaata caggtgctta tggtgggaat
     4201 ggtgtctctg tttcttccga atctggacac aataccttac caatatataa tgatattgat
     4261 gctgttagat ttaatgctag aacagtatat actaataggt taccagcagg agcacttaga
     4321 gggtatggtg ctacacaggg aatatttgca atagatagtg ctattgatga attggcacat
     4381 aagattggaa tggacccact tgaatttaga attaaaaata taatagatag gtataccaat
     4441 ggaaaagcag ctgaagacga aatattaagt tgtgagctga gacaatgtat agaaaaggga
     4501 aagaaattaa ttaattggga tgataaatat ccatgtaaag atataggaaa caacaaagta
     4561 agagcagtag gtatggcagt agcaaatcat ggttctggta taccgcgtgt agatatggca
     4621 acagtcacta taagaatgga tgaagatggc acatataaaa tattatctgg tcttgctgac
     4681 ttaggtcaag gggctgatac aattcaagct caaattgctg cggaagcttt aaatactact
     4741 gtagatagaa tttctattta tacaggtgat tcagacttat gtccctatga tacaggagca
     4801 tttgcttctt gtacgacaac ggttacagga aatgcaacaa taaagacagc aaataaatta
     4861 aaagatatat tactttcagt tgcagagtca aagctaggat ttaaaaaaga agatttagag
     4921 ttgggaaaag acagggtttt tgttaaaaac gataatagca agtttgttct attagaggaa
     4981 ttgggtagag aatctgttgg aggaatagga tttgctagtg atgatgtacc agtagttttg
     5041 gaagcaacag aatcttttgg catgttgaaa aaaataaaac catttatagc aggatttgca
     5101 gagatagaat tagataaaaa tacaggtgaa tataaagtca taaattttgc atgtgtacct
     5161 gattgtggaa cagttataaa tccaacttta gctagaatac aagtagaagg tggtgtgatg
     5221 atgggtatag ggtttgcaat gtatgaagac cctatatttg ataaagatgg tagattatta
     5281 acagatagtt tcctacagta taaagttcct acaaaaaaag aagttggaaa tatattggta
     5341 gattttgctg agaatttcga cccaggaggt ccttatggtg ctaaatcatt aggagaaata
     5401 gttatacata caccagcacc agctatagca aatgcaatat ttaatgctac aggagttcgt
     5461 ttaagagagc ttcctatgac ttttgaaaaa gtttatatgg gtatgaaaaa taataataaa
     5521 caattataat aaataataag atgcataaaa gcaagtaaat ttaaattatt ttgtaaaata
     5581 aagtaattaa atggacatta tatatggtgt tttgtaaaaa taccgtatat aatgtctagt
     5641 agagtatatt ctaaaggagg tgagttcatg tcaaacagaa taaaaaatat acactatgga
     5701 actcagagac aacaattaaa aaacttaact aaaaaagaat atcaagctct acgagagatg
     5761 tgcttcttag ctaagaactt atataatgtt ggtacatata atgtaagaca gcattatttc
     5821 aatcaaggag aacatcttaa ctatgaagct aattatcatg tatgcaaaga aaatgagaac
     5881 tataagcttc tcaatagtaa tatagcgcaa caaatattaa aagaagtaga cagttcgttt
     5941 aggtcatttt ttggattaat taaattagcc aaagaaggaa aatatgactt tagagggata
     6001 aaatcaccta aatatttaga taaagaagga ttttttagca taataatagg tcaaataaga
     6061 atcaaagaag atgggatttt aaatgtacct atgtctccag catttaaaaa actatatggg
     6121 aaagtaagta taaaagttcc acaaaatata ctagagaaaa aaattaaaga aataagaata
     6181 atacctaaac ataaagctag gttctttgaa attcagtatt gttatgaaat accaaaatca
     6241 gaggaagttt caaatcaaaa aaatgcacta gctatagact tgggtttaga aaatttatgt
     6301 acatgtgtta ccaatttagg tgatagtttt atcatagatg gaaaaaggct aaaatctgtg
     6361 aatcagtggg caaacaaaca gaatgataaa atagaagcct taaaattaaa aaagaatata
     6421 actgcaataa caagaaaaca atttaaagtt tggaataaga gaaataatca agttaaagac
     6481 tatgtaaata aaacctgttt ttatataatt gattattgtg taaaaaataa tattggaaat
     6541 ttaattgtag gatataatgc taagttagaa aaaagtgata atatggagag aaaaatatat
     6601 agaaatttta taagaatccc atttggagag ataaaaggaa aattagaata tctgtgtaaa
     6661 ataaaaaata tcaattttat taggcaagaa gaaagctata ctagtaaatc agattttttt
     6721 gctgatgatt ttctacctga tatcaatgtg gacaatgtta aaaaatatga ctttaaaggg
     6781 gaaagaataa cacgtggaca gtacaaatcc agtgttggaa tcattataaa tgcagatgta
     6841 aatgcgagtt ttaatatact aaaaaaatca caagtagtta atttatctaa tttacagaat
     6901 aatcctcaaa tattaaagca accacagaga gtaagaatat cttaattata tttaaaagat
     6961 aaactgatag cgtaagctta cagcttagcg gcgaattgga tccgccttgt tgtttacaac
     7021 agaatctatt tactttcgag tttatggaat ttcaaaaatc acttttacta aaaaggccta
     7081 actataataa ttaagagatt agaaaactgg ttataagagg tgaaagtgtg gaactatcaa
     7141 atataataga aaagaatgaa ataataactg tagttggagc aggaggaaaa acttcgttta
     7201 taaactattt tgcaaatttt tatagaaata aattgaaagt tcttcttaca acaacaacta
     7261 aaatatatgt accaaacgat tatgacaata taattattac aatagatgga actgttattc
     7321 catctatttg tcatggaata acagtctgtg gcagttatat aaataatgaa aataaattag
     7381 taagtattga ttcctctatt ttggatgaaa ttgtagacca atttgattta gttttaatag
     7441 agggagatgg ctcaaagaga aaaaaactta aaggttggaa tgctaaagaa ccagtagtat
     7501 atcatagaac tacaaaaacc atagggatat tagatataac atcatttgga atgaatatta
     7561 atgaagagaa tatacataga gtagaaatat ttaaaaaaat agcaaattta gatacaagta
     7621 gtattaatag tagttctaca gtaagtattg aaaacttaaa aaatatagta ttaaatccaa
     7681 atggattatt
//
LOCUS       DAMJYX010000133         7632 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJYX010000133 DAMJYX010000000
VERSION     DAMJYX010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.138"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            161..1564
                     /locus_tag="PC479_003170"
     CDS             161..1564
                     /locus_tag="PC479_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2805691.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1655..1895
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1938..2636
                     /locus_tag="PC479_003171"
     CDS             1938..2636
                     /locus_tag="PC479_003171"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2805692.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2659..3660
                     /gene="trpS"
                     /locus_tag="PC479_003172"
     CDS             2659..3660
                     /gene="trpS"
                     /locus_tag="PC479_003172"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2805693.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4312..5879)
                     /locus_tag="PC479_003173"
                     /pseudo
     CDS             complement(4312..5879)
                     /locus_tag="PC479_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5854..5997)
                     /locus_tag="PC479_003174"
     CDS             complement(5854..5997)
                     /locus_tag="PC479_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805694.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6215..6397)
                     /locus_tag="PC479_003175"
     CDS             complement(6215..6397)
                     /locus_tag="PC479_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805695.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(6786..7532)
                     /gene="cdtR"
                     /locus_tag="PC479_003176"
     CDS             complement(6786..7532)
                     /gene="cdtR"
                     /locus_tag="PC479_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2805696.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2726 a          912 c         1116 g         2878 t
ORIGIN      
        1 tttaataaga tttgatttca aagtatttaa ggtttattat taaaactgtt tttgaatatg
       61 caaatatttt gaaaaatatc agtttaataa taatgaatct attttattaa ttttgaaatt
      121 aaatttaaaa taattaatag agaggggaaa ttaaattagg atggaaaaga aaaaaatagg
      181 cctatggagt ttggtattta tgaatgtatc agctttatat ggtataagat ggatagctaa
      241 atctacagca tcaagctttg gtttaggatt aggtgcaata cctatgtggt tattattctc
      301 atttatatac tttgtaccag gagctttaat ttgtgctgaa ttagcttcta catatcaaaa
      361 aggtgatgga ggtctttacg attgggttaa ggaagccttt ggagaaaagt atggattttt
      421 agtttcttgg ttaaactgga cagctaaaat attttggtat tcttcatttt taactttctt
      481 agcagtaaat gtttcttata caataggaaa tccagccctt gctgataata agatgtttgt
      541 acttatatta tctttaataa tattctgggc attatctctt atagcaacta aaggaataag
      601 ttttacaaaa atatttacta atacaggtgc attaggttca acaataccaa gtgctatact
      661 tataatattt tcatttgtag cagtatttgt gttgaaaaaa catgatatag cttctacata
      721 cacaatagct tcaatgactc caaaacttaa tatggatgca tttgttgcta tatcagctat
      781 aatgtttggt ttagcaggag cagagactgc tgctaacttc ataacagaaa tagataagcc
      841 tgaaaagaac ttccctaagg caatattaat atcagctgga atagtagctt cattatatgt
      901 attaggttca attgcaataa caatgataat accaccagac caaattactg cttctaaagg
      961 tgtattagat gcactttctg ctgtttgtgc gtctttagga attggttctt ggttggttca
     1021 attaatagct tttggtatag ctttttcagt attaggagca atagttcttt acatagcatc
     1081 accaataaaa atgttatttg gtagtgttaa aaaaggaata tttccagata gtttagtaga
     1141 ggtaaatgaa cacaagatac cttctaaagc agttatactt caagcaataa tagttactat
     1201 aatactattg gtgactacat taatgccttc tgttgatgct atatacaatg ttcttgtaac
     1261 tatgacagca ttaactgcat tattcccata tgtattgtta tatgcttctt atattaaact
     1321 aagaaaagag agaccagatg aaattagacc ttatactatg gcaaaatcaa caagtacttg
     1381 cctaggttta gcaaaaatgg ttttagttgt aactgtagtg ggtatattat tatctgcagc
     1441 accagttatg cctacatttg ctgaaaatgt tatatatgag atagagatga ttggtggagg
     1501 tcttctagtt atattatctg gtctatggtt atggaataga tatgaaaaga aaacagctaa
     1561 ataaaatgta ttttaaaaat ttcttttgga tattataata tatagttgac aatttatagt
     1621 gtaaggaata ttataaaata aagaaattaa taaattaaat cctgagataa gaaatagtaa
     1681 ataaatatat ttttgcacag agaattaagg atgctgagaa cttaataaaa agtatttatt
     1741 gaatgggtct ttgaggagta aaatgaaatg taatttaata ttacagagta tttgatatcg
     1801 tgtatgcacg ttatagcata tattaagata tatatattca attttgagtg tatataaatt
     1861 taggtggtac cacggaaaat atatccgtcc tattgattta ggacggattt tttaatatag
     1921 aaaatttagg aggaaaaatg aacgtaatag gaattatagg tgcaatggat gaagaagtta
     1981 gtattttagt tgatttaatg gatattagag agactataaa aaaagctagc ttagaatttt
     2041 ataagggaat tttggaaggt aagaatgtag tgttagtaaa gtgtggcata ggtaaggtta
     2101 actctgctct ttgtgcccag atactaataa gcgaatttaa ggttgatgct attgttaata
     2161 caggtgttgc aggagcttta aacgaaaaac ttgatgttaa tgatatagta atatcaactg
     2221 atgctataca gtatgatgta gacactacag catttggaga ccctaaggga gttataccaa
     2281 gaatgaagac ttctgtattt aaagcagatg aaagacttat agatgctgct tacaaatctt
     2341 ctgtggaaga agtaaagact cataaagtat taaaaggaag agtagttaca ggagataaat
     2401 ttataaattc taaagaatta aaagaagaat tagtaaatga ttttggtgga tattgtggag
     2461 aaatggaagg tggagctata gctcatgtat gctatttaaa taatactcca tttgtaataa
     2521 taagagcaat gtctgataag gcagatggaa gtgctgatgt tacttatgat gtatttgtac
     2581 atgatgctgc caataactct aaagacatag ttttaaatat gttaaaatca atataaatat
     2641 ataaggaggt acaaaaaaat gagtgaaaaa aaagttatat ttagtggtgc acagccatca
     2701 ggcaaaatga ctttaggaaa ttatttagga gctataaaaa actggactga acttcaagac
     2761 aattatgatt gttattatag tatagttgat ttgcatgcaa taacagtacc tcaagaccct
     2821 aaagttttaa gagccaatac tatagaatta cttgctcaat atatagcttg tggattagac
     2881 ccagaaaaaa atactatatt tatacaatca catgtcaaag agcatgttga acttatgtgg
     2941 gtattgaata ctatgactta tatgggtgaa ttaagtagaa tgactcaatt caaagataaa
     3001 tcacaaaaga gtgaagcaaa tctaaatgca ggtttattca catatccagt tcttatggct
     3061 gctgacatat tgttgtatca aactgaccta gttccagttg gagatgacca aaaacaacat
     3121 ttagaattag ctcgtgattt agcaaataga tttaataata gattttcacc tacttttgta
     3181 gttccagaag gatactaccc aaaaggtgga gcaagagtga tgagtttgca agagccaact
     3241 aaaaaaatga gtaaatccga ttcaaatgaa aatgcattta tactattagc agatgattca
     3301 gattctataa agagaaagat aaaaagaagt gttactgatt ctttaggtgt tgtaaaatac
     3361 aatgatgaac aaccaggtat taaaaatcta attgatatat attctaattt aagtaaaaag
     3421 agtgtagaag agattgtaaa catgtatgaa ggaaagggtt atggcatatt taaagaagat
     3481 gtggctgaag tagtatcaga agctttaaga cctataagag aaaaatatgt tgaccttctt
     3541 aataataaag attatttaga aaaaatatat agtatgggag cagaaaaagc cgaaaaacaa
     3601 gctagaaaaa ctcttagaaa agtttataaa aaagttggat ttatagagag aagatactaa
     3661 ttattagcta tctttacttg ataaataaat tttaaagtta ttaaaaaggt atatgatatg
     3721 aaagagagag tctcaaagtg aacttttgtt catgaggcac tcttttttgt atgaaataaa
     3781 atatatttta atcatttcaa caatgaaaaa gaggattatc tctattttga gacagcatct
     3841 tttgataata aaaaaagatg agcttttgct catctcaaag gggttagatt attcttactt
     3901 tggggaaagt agaataaagg taaaagttaa ctgatgtcaa ctttgtcttt tttgcatatc
     3961 actactgagc ataaaaaaat cagaaaacca tagccttctg aaacaacttc taacatacat
     4021 tgtattttat acccaatatg taatttatga gttaatttta actcttataa tgaaaattgt
     4081 caataatata tcaaaaaaat attttttaaa tttatataat taaatgaatt tatgtttata
     4141 tataggtgat atgatacaga taattatctt tttaatacaa tatagttcta aaaaagaata
     4201 tattagaaga agataatatt taaataaaaa ataccttata aagttaattt tttaatactt
     4261 tataaggtaa atacatattg tattgtgggg ataaatttaa atcatttgat actaatcaac
     4321 actaagaacc aataactctc tatcatcttg agtaattgta tatattctta atttcttgta
     4381 tgtcataata ttttctccac ctgtaaaata actcctaaga ttaacacaat cagttttagg
     4441 ttgatttgta tctcctaaat aattacgcaa ttctttatct ttagtttcaa tatctttaaa
     4501 tccaaaatat tgtaaagtag cttcaactct atatttttgg acataatcga gagcttcttt
     4561 attagtttat gttggtgtaa aatacatacc atttatataa gcctttattg tacttggatt
     4621 aaatttaaaa tctgcagaat atattttatg tgtaactatt atttcttgaa cagttggaat
     4681 tttaacttct ggttcatcaa gtatttcagg agtactattt agctctgtaa tagataagtt
     4741 atctaagtat attgtatcac tatcaattaa tgctatattt atattagaag aatctttttc
     4801 agtagtttca aattcataac taaattttgt atatccttgt tctagtacca aataatccgt
     4861 tttttcttct tttactttta tatttacaat tattgaatta tatgttaaag gattttttga
     4921 attttactaa aaacataacg tttataaggt tttagttcag acataggtat tttaatcttc
     4981 gtctcaccat ttaatctatt tgctgagcct tttaaaccat cttgatttgt agtattaaca
     5041 ttactccata tactagggta attattgtaa tcatcaaaat tagtaaagta agttggtgtt
     5101 tttataagta tattcattcc tctttcaagt ttaacattac atatttgttt atcagacaaa
     5161 gtttttaaac tgtctttaat cttattgcct atattatcat caaatatgag ttcaacacaa
     5221 ctttcatcta ttggtatatt attaaaatat aacaaaccat cttttttagt agtgccaaaa
     5281 gttttttcaa ttgcttctcc aattgtaagt tcaggtgttt tatcttctgg gacctgtaaa
     5341 cttttagcag taattcttct tttgtaagat tcattttctg tatctaatat aatagataca
     5401 gaaatactgt caatttgacc tatatcgtct taccaactat ttcattctgt tactatttgt
     5461 ccagaaccat tttttgtacc aaaatttcca cttacttgtg ttgtttctaa tttaatttgc
     5521 tttccatcat ctaatttttt taattaatta taatttatag gaatcagtct agaactaaat
     5581 tgatacattg tgttaagagc cataggtgaa aatccttttt tgagataagt atttccagga
     5641 gatagattat tgccaatttg attttcttgt gctttgatag ttgataatgc atctccatct
     5701 aacactaaat ttgttgttgg tgtcactttg tacataggtg cagtacctgt gttgtagtat
     5761 ctaatatttg catttatata tgcagattct cctttgttta tacttaatcc agtatttcat
     5821 gattatccat tgctattttg aacagcagtt gaattatctg ttgtatggta ataatttgtc
     5881 ttgtatagtt tctttaaact cattaaattt ttctaatgaa atttcgtttt gattttttgt
     5941 tcttattact ttattaaatg caaatttttc tggagattca aaataatata catacatagg
     6001 tttatctatt ttattttttg atatagcatt tcgaagttct ctatattctt tttctcaaga
     6061 atttgcttct atttgataat cataaaaata atttcttgtc tgagaatatt tgcttatctc
     6121 tatagaatct tttttatatg atcctaatac ttctttttca gatttttcaa gtttttgatc
     6181 ttttctttct cattctttag ccttttcttt atctttaaaa aaatccttct gatctttcta
     6241 taggagcctt gtaagtagta ttacaaactt ttccactata gctttgtaaa tcttgtgcat
     6301 aaatgttatt aggtaacaaa ctacctaaag ttagatataa tatcaacaat atagatatac
     6361 aattactaat acttttatgt ttcctaaatt ttttcattta ttctccctac aaatattagt
     6421 ttagttaact tttgaatttt aatttattta gattatataa tctaaataag aattttttat
     6481 ataaatttca taaaatatgt aggtatatat cataaaaata tgtatttata tgatatgtca
     6541 gtacaaaata acaactgaat attttaaaga gactatttcg tacttttatg atttaatgag
     6601 ctatcttatc gaatcataat ctaggaacaa ttgttataat atctaatagt agttatatat
     6661 gatatagtca gtatgtgttt tttcacaata tgattattat atagaggatt cttagggatt
     6721 actctttttg tataaaaaag agatggttca aatctttatc ttgagacatc tcctttttct
     6781 atttattatg ttttaataat attctttaaa atatttctat atgtattaga tatacgacat
     6841 atatggccat tactcatttt aatattattt ttctttaggt cgagagaaac aatatgatat
     6901 ttattaacta caaaagattt atgtacacgg ataaagcatg catctaaatc tggtaaagac
     6961 tttaaacttt tatagaattc aaatattcta gaagtagtca ctaattttat tttatgtgga
     7021 atagtgcttg tttcaaaata tataacttca tcaaatgaaa ttttccatag tgagaaatct
     7081 gaatagatag taaaaaaatt agagcgataa tttttttctt taatgtatct tttgtgagca
     7141 attttaatac aagcttttat tttactttca gcaatttcca tatcatgttt tagtataaag
     7201 tccataactt ctaacctata atcgaagatc cctaaaatca tttctatatg atttgtaaga
     7261 aatatgattg gactaatata atctgattct ctaatttttt gtgctatcca taatccatca
     7321 cataattcat tacttatctt atattttgcg tctacaacta taaaatatat tcttacttta
     7381 tttttttcag taagctcttt taataacaaa tttgcattat gatataatct tacaacatca
     7441 ttatcaaaac cttcttttat taaaatatta tttatttttt cttttagttt cattccaaaa
     7501 caaacatcat tatcaaagat taatatatcc actaaatacc ctcctataaa aaattcaaaa
     7561 gtaaaaaaac aaaagtattt tatagaaaat acataaaata acactaggag ataaataaga
     7621 ttaaggatag tt
//
LOCUS       DAMJYX010000134         7485 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJYX010000134 DAMJYX010000000
VERSION     DAMJYX010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.139"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            315..1127
                     /locus_tag="PC479_003177"
     CDS             315..1127
                     /locus_tag="PC479_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2805697.1"
                     /translation="MYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            2176..2937
                     /locus_tag="PC479_003178"
     CDS             2176..2937
                     /locus_tag="PC479_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2805698.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            3002..3784
                     /locus_tag="PC479_003179"
     CDS             3002..3784
                     /locus_tag="PC479_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2805699.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            4051..4470
                     /locus_tag="PC479_003180"
     CDS             4051..4470
                     /locus_tag="PC479_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2805700.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4573..4839)
                     /locus_tag="PC479_003181"
     CDS             complement(4573..4839)
                     /locus_tag="PC479_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2805701.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            5066..5767
                     /locus_tag="PC479_003182"
     CDS             5066..5767
                     /locus_tag="PC479_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805702.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5849..6457
                     /locus_tag="PC479_003183"
     CDS             5849..6457
                     /locus_tag="PC479_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2805703.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            6781..7245
                     /locus_tag="PC479_003184"
     CDS             6781..7245
                     /locus_tag="PC479_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF2805704.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
BASE COUNT         3062 a          671 c         1261 g         2491 t
ORIGIN      
        1 tttattgaca aatataaaat ttaaatatat aattaattca taaaataaaa atatgaaagg
       61 atgatgttgt tcggtgatat ttgatatcat aaaatcgttg atataagtat tgctggaaaa
      121 gagtgaataa aatcaaattt aataagcaaa caaagtaaag ctatagtttt attgttgtgc
      181 ttaaaaatat ttggttctat tttactctta tttaaactca tctttttgaa gttaaaccac
      241 agagtaaact ctgtggtttt tttattttaa aaataaattt taaggagtgg tatgttttgt
      301 taaacaaatt atgtatgtat ttagaaagac cagagcttta taaacaaagt gaaattaatt
      361 tttgggatga tgagtatatt tcagagcaat tattaaaagc acacttagac actaattttg
      421 aaggagcaag tagaaatttt aactttatcg aggattctgt aaattggata gttacagttg
      481 caaatccagt taattatcct aaattacttg atttaggttg tggtccaggt ttatatgcag
      541 agaaatttgc acaaaaaggt tataaggtaa ctggaattga tttctcaaaa cgttctatta
      601 actatgcaca aaatagaaat aaggagacta atttaaatat aaattacttg tttcaaagtt
      661 atttaaatat gaactataat gaggaatttg atttggctac cttaatttat tgtgattatg
      721 gagctttgtc aactgaaaat agaaggttat taatggaaaa aatttatgat agtttaaaac
      781 ctggaggaaa attaatatta gatgtattta caataaataa atacaataat tttgaggaaa
      841 ttaaatattg ggaaataaat gaagatggag gattttggag taatgaaaaa tatatgtgtc
      901 tacaagataa ttgtaaatac aatgattata atacattaga acaaactctt gtaataaccg
      961 aaaaagatga aaatgtattt tatgtttgga atcactattt ttcaaaagaa agtttcttat
     1021 tagaagttga aaatattgga tttaagtctg ttgagttttt tagtaatgtt aagggtgaag
     1081 catattctga cgatagtatg actatgggat tagttttaca aaaataataa tttttttaaa
     1141 atgaataaat aaaaacttga aacctttaac aataaaataa cataagaaaa gcttacatat
     1201 tgaaaaatgg aaacttttat gttattttta gtacattaca aatttgttta tataaaaatc
     1261 aatacttgaa ttattatttt tatgtgaaat ttatctatag tatattttta taaacaagta
     1321 atgttattaa aattaagtat atagttaaaa gtgtatagca aaaaggctgt tatattaatg
     1381 ttagaggttc agggaaaata tataaagcaa agatatttac agataatgta gattgcacag
     1441 ctataagtta gataatagaa ttatgtaatc aagaatttac aacaaagtat aagataagga
     1501 taatgctaaa ttgccataca ggtgctagat gtgtaaatgg atttaattaa tactattgat
     1561 aaagtgcaac aagcacataa accaattgat gagattattg aaactataag agatatagtt
     1621 gacataatta aaatattgga acctgtttat aattttaaat caaatcaata atgattaaga
     1681 ttaaaataat attaaggcat caatgttata agagtacaat ataaggagta ctatatagta
     1741 ctccttactt atacatatta ggcaataggg aattttttta tagttaaatt ttcaaatatt
     1801 ttttagggaa ctttataatt gagaattaag ggtatagtat gtgatgaact tactaaattt
     1861 ataattaggt tatataatgg aataatcatc aatagtttta ctttagggaa aatatatgct
     1921 taaggaagta tagcttacta atattattaa aatgtatatt taaaatgata aaaaaagaat
     1981 tagacaagta tttagtaaat tgactatgca aaattctatc aagcaggaac actatgactt
     2041 tagtcatggg aggttcagta ggtaacttgt tgaattaagt ttatattatt taaataaact
     2101 acttaattta gataatataa aaagtcagaa ttttaagaat agagctatat aatcttagct
     2161 attaggagaa gtactatgat aggaatatat tttagtggaa caggaaattc aagatactgt
     2221 gtagaaaaat ttttacaaga atatgatata acagcaaatt cttattctat tgaagataat
     2281 gaacttttac agcacatcaa taatcatgag aatattattt ttagttatcc tgtgcaatat
     2341 agtaatgtgc caaaaatatt gaaagatttt ataataaatc attcaacttt gtggaatggt
     2401 aaacgtgttt ttattattgc aactatggct ctatttagtg gagatggctc tggtgtgtta
     2461 gctagaatat taaaaaaata cggtgctata actgttggtg gattacatgt gaagatgcca
     2521 gatagtatag gagatgaaaa ggtattaaaa cattctttag agtataatag aaagcttgta
     2581 ataaaagcag aaaagaaagt aactaaagct gctaaaaaac tgaaacatgg gtcaccacca
     2641 aaagaagggt tgggtttttt gagtcatatt gtagggttat ttggacaacg attgtatttt
     2701 atcaataaaa caaatatgta tacagataaa ctaaaaatta atactgagga atgtattgta
     2761 tgtggtaaat gcgttaattt atgtccaatg aagaatctag taattaaaaa ttgtatgatt
     2821 gttgcaaatg ggcaatgtac aatgtgttat cgttgtgtaa ataattgtgc caaaaaagca
     2881 attacattgt taggtaaaaa agttgttgaa cagagtaata ttaaaaaata tatgtaagaa
     2941 atttaaagtt ttattgaatg taaattgatg tatattttat gttagaaaga gaggttaaat
     3001 aatggaatac tacatagttg attcatttgc aactaaacta tttaaaggaa atcctgctgg
     3061 agtttgtgtt cttgacagaa gaataccttt agagttaatg cagaaaatag cagaagaaaa
     3121 caatttacca gaaacagcat ttgtagttaa atgtaaagga aattatgaat tacgatggtt
     3181 tactcctaaa gcagaaattg atttatgtgg tcatgcaact ttagcagcag cttatgtaat
     3241 atcaaatttt attgatgtta atgtaaaaag gatagatttc tttactcaaa gtggaaagct
     3301 tgaagttaca cgaaatggaa atctatatga aatgatattt ccagaaataa tgcctattga
     3361 aatagagtta tcaccacaac aagctaattt gataggctgt gtaccaagtg ctgtttattc
     3421 ttcaagagat ttaatcttat tgctaaacag tgaacaagaa gttataaatt ataaacctaa
     3481 ttacgcacaa ttgcgtaaat tgacagactg gttaggtata ataatcacag cacaaggaag
     3541 taatacagat tttgtatcaa gatatttttg tccagaatta gattcagaag accctgttac
     3601 tggttcatca cattgtaatc ttattccata ctggtctgaa aagttgggaa aacacaaaat
     3661 ggtagctgct caactttcta atcgtggtgg tataattcag tgtgaagtgt tgaaggataa
     3721 tactgtaaaa atttctggtg aagcagtctt gtttatgcag ggaacaataa agattgatat
     3781 ttaaataagt ataataacta atttattatg aaaaataatt aattttctta gtgttaacaa
     3841 taataaagta gagttaaaaa gcttgatttc acttaaatca atatgataga tgttgatact
     3901 aagattgaca agcatgatta aaaaagataa tatttaatct ataaagataa aaacacagtc
     3961 tggttggtag tccagacgca gcaatagctg tcagtaacct gcctccttgg ttgtccgttc
     4021 tttagaaagt aatttaagga ggaactagca atggacaaaa ttagtggaaa attaacagtt
     4081 ttatttgaag aaccattttg ggtaggtata tttgaaagac aagatggtaa aaaatatgaa
     4141 gcatgtaggg tagtttttgg agcagaacct aaagaagtag aagtgtatga atttatactt
     4201 gaaagatttt tctcattaga ttttggtagc attaaattag aaaaaaatgt gaccaaagac
     4261 aacattggat acaaaagaat gcagagaaaa gtaaaaaagg aacaagaaaa agaaactata
     4321 gggactaagg ctcaaaatgc tcttaagctt caatatgaag aaagaaaaca ggatagaaaa
     4381 aacctagcta aaaatagaaa agaagaagaa aaagaaagac tatttaattt gaaacaagaa
     4441 aagagaaaag ctaaacataa gggtcattaa ttttaattaa atatcttttt aataattgct
     4501 agaaaaaggt gtataaaatt tttaatttta tacaccttaa tttattgaaa agaataaggt
     4561 tttatatttc aattacttta aaatataaaa atatactaat aaaaattgta aaaatagtat
     4621 aaatggtata ccaaatgtaa atttaatgtg tttagtttta tgtctaaaag aatacattcc
     4681 aattatagaa cctatactac ctccaattac tgctatagac ataagagtag cttcttttat
     4741 tctccattcg tttctaattg ctcttttctt atctatgtac attgaaaaga aagcaataga
     4801 attaatcact ataaaatata ttagaataaa atttttcaca gtcttgcctc cataaaaata
     4861 tattatacat tataaaacaa gatatttgtt ttggaaagtt aaatttgtaa ttagtacata
     4921 taaaatattt gataagaaca aatataatat taattggaaa atttaaaaaa attaaacaat
     4981 tagcaaatag ggggtatatt tataagacac aaagtgataa aatctattta gaaaataatg
     5041 taaaataaca ggaggataaa gcaaaatgaa atttatagta gaaaaagaag tttttgataa
     5101 attagaaaat gtatgctttg gtgtagtagt tgcaaaagga atagataata ctaaagaaat
     5161 agaaagaata agcaaccttt tagatataag tatagataga gtagaagatt acttcaaaga
     5221 taaaaaagta aaggaatcag aagaaattat accatataga gaggcattta gaagtctagg
     5281 catgaaccca aataaattta tgagttcgat agaagcaatg acaacaaggg ttgctaagaa
     5341 taagaaactt cctcatataa atcctattgt agatttagga aactctattt ctttaaaata
     5401 tttacttcca atgggtgctc atgatatgga ttttagaaat gatgatgtat atgttagatt
     5461 ttctaaaaaa ggggataaat ttgtaccatt tggagaaact gatgttgaat taatggaaga
     5521 aggagaatta atttattcag ttggagacat ggtaaaaact agaagatgga tatggagaca
     5581 aggagaggaa ggaaaaatta ctaatagctc aaaaaatata ttttttccta tagatggttt
     5641 tactgatgct aatttagata aggtaatgag tgcaagagaa gaattagcaa aattgttaaa
     5701 ggaaatattt aattgtgaaa ttaaagtagg ttttgtagat aaagataatc ctgaaatgga
     5761 aatataataa gttgttgaat acttgttaag atattatatt tacttaatga tgttaaaaag
     5821 gtattaaaaa agaaaggtcg tagtaaattt gaaaaaaact ataataaaga atgtaaatcc
     5881 aatgattgaa aataagttta taggactttt tgaaatagag tataaaaaca aattagatga
     5941 agataaggtt tggatggtag catcaagaaa aagtagtgag caattaaaaa atatttattt
     6001 gaagaatgaa gaagatagtg tagatgcagt tgtaatagtt gggcttcata aaagtagtaa
     6061 aaaattaatt ttaataagac aatttagagt acctattaat ggatatatat acgagttacc
     6121 agcaggtctt gttgatgaag gagagtctat agatatttcc gttgaaagag aacttagaga
     6181 agaaacagga cttactcttt tagaaattaa aaaaaataaa agtattgata aagtttattt
     6241 atctcctgga atgagtgatg aatctgttgc ctttgtttat tgtatatgtg atggtgatat
     6301 aacagatgaa tttttagaac cagatgaaga gattgaagcg cttttaattt cgcaagaaga
     6361 tgcaaaagag attttacaaa gtaatcataa gatagacaca aaagcatttt tgattttaca
     6421 aatgtttgtg agtttaggag ctaaattatt tgaatagata tatagaaaag aggttatata
     6481 gaagagtaaa ataataaagt ttattttact tgtataaatt ataatctctt tttttataca
     6541 ataaagtata attttgtgta tttttaaata agtgacttga ctcttaaatt ttcataatat
     6601 gttataaacc aatttaagat actacaataa ttaaaattcc aaaattattt aacatatttg
     6661 caattaaaat gagaaaaggt aaaggttgaa tgattgaaaa atttgatgta aaatagtagc
     6721 ttagtttaat attatagttt gtagggttaa aaattagaaa ttgtaaggga gataaattct
     6781 atgaaaaaat tagttattat tttaattagt atcattagta tggttagctt gacagcttgt
     6841 aaaaaaagca atgaaagtaa atataataat ttaaacaaca aagaatccgg acaaacaacc
     6901 attgaaatgg acttagataa aaattatgat acttcagacc catttgttaa tgcacgtttg
     6961 ttttgtgtgt ctaatgatat agatgtttta gacacagaga tttcttttaa aatggatgga
     7021 gatagtggta ttgtggaaat aaaggacaat aaaacatatg aaactttatg gagtaataca
     7081 tggcatggaa gcgttgataa tgatacattc acaatttctc tttctaatat acaaaaagag
     7141 aaagagtatg ctatatgctt tactggaatt aaaataaatc atgcagttgt aaaggttact
     7201 tttgaaagca atttagttaa ggagaaagaa aaaccctcaa aataaattcc agattgtcta
     7261 taacttgata ttcagtacat aaaagcagtt gatatttatg attgactgct ttttcttatc
     7321 aaaaaggaga gtttgtgcat ttttacttga aatagtttat gcacatcaaa agttggtgag
     7381 catgatattg gaaatggtaa gcatgaaatc ttgataagac aaggaaataa atttaagttt
     7441 tcaattaagt atatttccat aatttagtta acaataagct aatta
//
LOCUS       DAMJYX010000135         7361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJYX010000135 DAMJYX010000000
VERSION     DAMJYX010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.140"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(268..1107)
                     /locus_tag="PC479_003185"
     CDS             complement(268..1107)
                     /locus_tag="PC479_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2805705.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1131..1889)
                     /locus_tag="PC479_003186"
     CDS             complement(1131..1889)
                     /locus_tag="PC479_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2805706.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1936..2409)
                     /locus_tag="PC479_003187"
     CDS             complement(1936..2409)
                     /locus_tag="PC479_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2805707.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2438..2854)
                     /locus_tag="PC479_003188"
     CDS             complement(2438..2854)
                     /locus_tag="PC479_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2805708.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2901..3953)
                     /locus_tag="PC479_003189"
     CDS             complement(2901..3953)
                     /locus_tag="PC479_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2805709.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4349..5029
                     /locus_tag="PC479_003190"
     CDS             4349..5029
                     /locus_tag="PC479_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2805710.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5162..5482)
                     /gene="sugE"
                     /locus_tag="PC479_003191"
     CDS             complement(5162..5482)
                     /gene="sugE"
                     /locus_tag="PC479_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2805711.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5864..6691
                     /locus_tag="PC479_003192"
     CDS             5864..6691
                     /locus_tag="PC479_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2805712.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
BASE COUNT         2506 a         1321 c          846 g         2688 t
ORIGIN      
        1 ataatatttt aattaaatta aataaattta tatttttttg aaattactta atattttata
       61 taaaaatgaa aaagcacctc attctgatta aaaatctttc tgagatactt tttaaaatat
      121 taaatactac atatagctta cataagttta aaatttaatc tattataaaa ttaccctaat
      181 tgccaaactt tttaatataa attttccaac atcctacaaa tataatcaat agatatagaa
      241 tcctaactta caccaatata tatttattta aaatagtcct aaaatacctg taatagctcc
      301 cactactatc agtataaaca ttatttttat tggagaaaga ccttttttta atgcaaagag
      361 tactaataaa gtaagtacca aaggaattaa tcctggaaat atcttatcaa gtaaatccgt
      421 ttgaagacta aatttaaaat caccactagt ataacttatt ggtgtagata aattgataaa
      481 cctacctaca agccccccca tgactaaggt acctagtata gaagcccctt ctataacctt
      541 atttactatt ccaccttgaa gtatactttc aacagctttt ttcccatact tatatccatt
      601 tgtccacatt ccaaaagaaa ttgaagtcat tattatatat tgagctatta caaataatat
      661 aggtcctgca attgatgctc ctccctcagt taaaccaata caaacagcta acactatagg
      721 agttataact ccttgagtta aagtatctcc aattcccgct agtggaccca tcaagcctgt
      781 tttgatagca ttcataccat ctccacttat ttcatctgct ccattagctt tttcttcttc
      841 catagctatt gttattccat gtataacaca tccacatata ggttctgtgt taaaaaatgc
      901 tagatgagtc tccacttctt gttttaactc ttccttatct ggatataatt ttttaagtac
      961 tggcaacatt gaatgtgaaa aagctgttga ttgcaatctt tcataattgt aattagattg
     1021 tgcaaagaaa aaccatctta accatgattt tattacatct ttcttgctta acgttttctt
     1081 agatacttca ttttttatat ctgacataat attttccccc tcacatgtat ttaagatgta
     1141 gcactatctt ttccgtactt aattgaagta attgtaagag caagtattac tccaataaca
     1201 gatattacta ttatatctac tttaaaataa acagccatta gtattcctaa tatgaaaaag
     1261 ggcatagtaa acttatttcc aatcaatttt aaattcatag caatcccaag tgctggcaac
     1321 atagccccaa ttacattaaa tacatgtaac acattatcat tcattacttc caacatacca
     1381 tctatcgcat ttggaccaaa ataaacaact aataatgctg gtataaaact cattgcaaat
     1441 aacagtatct gtggtggtac tatatttata aatgctactc tatcaacatt ccctttttga
     1501 acctctctat cagcccaatg tgcaaaaaaa gaatttactg tcatctttcc aatccaaagt
     1561 acagttgcaa cagttcccaa tggtacagct attgccattg cagtacttac atctagctta
     1621 gatgctatag ctaaagcagt acctacatat cctgcaaaag ctgggtcccc catctgagct
     1681 ccccctgctg atataaaacc tagatatatc atatttatac ctgctcctat aattgctcct
     1741 tgaacagggt ctcctaatat aattcccgtt acaaatccag ccaatagagg tctattccat
     1801 gtaaaaaatc caacataagt caaccacgga ctccaagaaa gataataaaa aattgctatt
     1861 aatactgctt gaattagtga aatgtgcata ttcttcctcc ctattttctt ttaaagattt
     1921 acaagatagt cttctttaca ataatgttcc tacatcaatt ttctttgcat ctggtactat
     1981 ttgtataaag catttaacat cttttgatat tagctctcta aatatttctt tttcttcatc
     2041 tgacacagat atatttttat aaaaactttt tctaccaagc cctgctccca tacctccaac
     2101 attcaattcc tttaaatcta ccccactatt tactaaacct aagacagttt tagggtattt
     2161 gaccaatact attaaatttt catctccatt atgtgatttt aagacttcag cacctgattc
     2221 tacatcatgt acttcaacct taacagttga tggagctgcc atttgtaaaa cttgaactat
     2281 aaatgggtcc tttactacta aatcatccac gattactatc ctttttgctg atgtaatctt
     2341 acaccaagca gtaataactt gcccatgtat taatctatca tcaattcttg ttaatgctat
     2401 attactcata attattcctc cagattttta atttatttta caactttaaa acagttttaa
     2461 catctttgat tcctgatatc ccaacttcct cacataaatt cttcaagtct tttaacttca
     2521 tagtatctct gtttaaaaat aactctaaaa gcataggtaa atttacacct gtcaaacatt
     2581 ctaactcaca taccttagca aatttattta ttacaatagc agatgtatta aatggagttc
     2641 cgccataaaa atcaagcaat actaaaaccc cttcatcctt atatttctca attccatctt
     2701 ctaactttct actaaactca tctatgctgt caccatggtt taatcctaaa gtttctacat
     2761 tttcctgttt gcctactatt aattcagcac tttctaacat tgctttagcc atatttccat
     2821 gtgtggctat aattattcct gccatatgaa tcataccttt cttaaccttt tttatatgga
     2881 ttattattta gtactctaat ctaattatct atattaatca ttatcttacc aaagaaaata
     2941 tcccttttgt atactttttc aaaataagct ggtacttctt ctaaatttat tctatgtgtt
     3001 actaattttt caacattcat atcacccttt tgcatatgat acaatccagc actccattct
     3061 tttcctggga atggaaagga gtttccaaac caagttccct ttacagttaa ttcacttctt
     3121 actatctttt caaattgctc tctagtcaaa gctacatccc catatggtac acctgcatac
     3181 aatactgtac ctcccttttt agctagcaac agtacttgac cacatgtcag tggagtacca
     3241 gccgattcaa taacaatatc tgcaccatct ccatcagtta acctttttat ttcttctaca
     3301 atattctttt cttttgcatt tatacaaata tctgccccta actcttttgc caagtctaac
     3361 ttctcatcaa atacatccac agctattatt tttgtagaac caaatatttt tgcccattgt
     3421 attgaaaata atcctattgg acctgtacca agtactacaa cagtatctcc aactttagct
     3481 tctgacctaa aaagaccatg tcctgctata catactggtt ctagcgcagc ggcagtctca
     3541 taacttatct catctggtat ttttattaaa tttctctctt tcacctttgt atactctgca
     3601 aaacaacctc caagttcttt atttcctatt attgctacat tattacatct tgaatacaag
     3661 ccttttttac attcatcaca ttcaaaacat ggcatagctg gacaaacagc aactctatct
     3721 cctattttaa aactccttac ttctttacca acctgtgcca cttctccaga aaattcatgc
     3781 cctaaaattt ctccaaccat atgtggacct gtctttgaat attttgatat atcagaacca
     3841 catattccag ctacttttac tttaattatt acatcatctt tttctaatat ttttggtaca
     3901 tccacatcct ccactcttgt gtctcttata ccatagaatc taactgactt cactattaac
     3961 cacccctact ttttatttga gaatatatcc ctattgttta tatgttaaat atctaaagca
     4021 actttcatgc caatatatgt aagcattgta ttcatgcatt ttataaaatt atcattaaat
     4081 gtgtgtcaaa gtgtgtcaaa agtttcttat attaaaactt atgtgtgtca atataaacga
     4141 cactaacatt ttatattagt tctcttatta taagttttat aaaatgtact ttttttgtta
     4201 tttttataaa attgttcgga aatttccctt ctatttgtga tataatatta aatataaggt
     4261 atcaatataa attttcatgc gaatatcaga acttttttga cactaatgcc ttattcaata
     4321 taataattta actttttgga ggttgtttat gaaaaaagtg tatcaaggaa agactaaaga
     4381 tgtttttgaa ttagataatg gaaattactt acttaaattt aaagacgatg taactggtaa
     4441 agatggagta tttgacccag gcgaaaatag tgtaggtcta agtatagatg gaattggtag
     4501 agctaattta gagacatctg ttaagttctt tgaaattcta aacaatgctg gaattaaaac
     4561 tcattatgta agtgctaatt tagaagaagc aactatggaa gttctaccag caaaagtttt
     4621 tggtaatgga ttagaagtta tttgtcgtta tagagctgta ggtagtttct tacgtcgtta
     4681 tggttcatgt gttgaagaag gtgctaaact agattgttac gtagaagcaa ctttaaaaga
     4741 tgatgaccgt tgtgaccctc ttataacatc tgaaggttta gaagctttaa acattatgac
     4801 tcaagctcaa tttgattcaa tgaaaagcat gactcaaaaa atttctaaca tagttcgtga
     4861 tactatagct gaaaaaggtc ttgagcttta tgatattaaa tttgaatttg gattctacaa
     4921 tgatgaagta atcttaatag atgaaatagc atctggaaat atgcgtgttt ataaagatgg
     4981 tgtaatagtt gaccctatgg atttaactgc tcttttatta gataaatagt atatacatat
     5041 aatttacaaa tataataaac ttataaataa gataagctat gaagtaaaga ctttttaaat
     5101 gttgattatt tttaaacatt ttctcgcctt taatttcata gcttatttaa attaatatac
     5161 tctaattagt agataatagc tttaacccta caattcccac cacaataaaa cctatacatg
     5221 caattcttga tatactaata ggctcattta aaatagccat gcctacaata gctgtaccga
     5281 cggtaccaat tccagtccat attgcatatg aagttccaag tggaagatgt tttagtgcta
     5341 atgacaaaaa ataaaaactt acaatcattc caacaacagt aagtatactt ggagttaatt
     5401 tagaaaatcc atgtgaattt tttagttgaa aagcccaaaa cacttcaaac aaacctgcaa
     5461 caaacaaaaa taaccatttc ataaattcat ccctcttttc aaatatgtct cttatttttc
     5521 caataaaata acctgagaaa tattataaaa tatctcccag gtttttgtcc ttccgtgttc
     5581 acaacaaaaa ctatgttgtg ggttttatct cggaccagac caatcattaa taattgcgga
     5641 accctataaa actctattaa ttattctatt ttatctataa taatactatc aacatatata
     5701 aaaaaagtca atatatgggt tccatatata ataaaaataa cttttatata ccttttaata
     5761 ttcttattca cttctctcca tacccatttg ggtcaatcta agaatatcta atttttattt
     5821 aatatgttat aataacataa aaatattgaa aggagtaatt tctatgaatt tctataaaat
     5881 tgcagtatgt cagatgatta ctacagaaaa taaaattgaa aatatcaatc atgctgtaga
     5941 tatggtaaca gaagctgcca tcaatggagc taaaattgta gttttgccag aaatgtttaa
     6001 ctgcccatat gaaaataaat attttcctaa atttgctgaa gaatatccag gtgaaacaac
     6061 aacgatatta agtaaattag ctgagaaaca tggaatttat ctagtttctg gtagtattcc
     6121 tgagttagaa gatggaaaaa tctataatac ttgctatgta tttgataaaa atggagctct
     6181 aattggaaaa catcgtaaaa tgcacttatt tgacattgaa gttacaggaa aagttagttt
     6241 taaagaatca gatactctta cagctggaaa tgatgtcact gtaatagata ccgaatatgg
     6301 aaaagttggt atagctattt gctatgatat tcgtttccct gagttatcac gtttaatggc
     6361 tcttaaaggt gctgaaatcg ttatcttacc tgctgcattt aatatgacta ctggtcccgc
     6421 ccattgggag ttatctataa gaatgcgtgc tttagataat cagatattct atgttggtgc
     6481 tgcacctgct agaaatatga atgcttctta tatagctttt ggaaattcaa gaatttcaga
     6541 cccttgggga agaataattg ctcaagctga tgaaaaagaa tgtattattt atgctgacat
     6601 tgatagagat ttaattcctg acataagaca acagttacca cttcttaagc acagaagaac
     6661 tgatttatat gaacttaata ctttgaaata aaacagaaca taaccccata aatcataaat
     6721 taagtgttaa aaaaatttat ggggttctca ttaatttgat ttaaaattaa tttcataaaa
     6781 ataatgctaa tattaattta attaaataat actaataatt actaatgatt atgctaatta
     6841 tcagcattta ctaatttaat cactattagt tatttctttt attaaatatc tttacttttt
     6901 tacttattat gtcttctagc tttacatttg gattatggta tttctttcta tttttagtca
     6961 catctttcta ttgaattact tgcttaaaac accaatgcac acatgccaga taatgttcac
     7021 aattatttag aaatcttgct ctaccttctt caatgttgat attatctctc ctagttgata
     7081 ttatctttcc taaaatttct tttaattaaa aaacaacatt agttgatata tcaagtatat
     7141 tttaagcaac tgaaaaaaac aataataatt gatatatcaa ctaattaatg tagattaatt
     7201 catttttata atttatatac tttttaatta atctacctta tttaattaaa atccttcctt
     7261 tctatttatc cattttcaac ttaaattata agatttcatc agctatattt ttgcatacca
     7321 ataattatta aatatcaaat atatattcta ttaaaatttc t
//
LOCUS       DAMJYX010000136         7141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJYX010000136 DAMJYX010000000
VERSION     DAMJYX010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.141"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            457..966
                     /locus_tag="PC479_003193"
     CDS             457..966
                     /locus_tag="PC479_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2805713.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            994..1188
                     /gene="rpmI"
                     /locus_tag="PC479_003194"
     CDS             994..1188
                     /gene="rpmI"
                     /locus_tag="PC479_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2805714.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1244..1600
                     /gene="rplT"
                     /locus_tag="PC479_003195"
     CDS             1244..1600
                     /gene="rplT"
                     /locus_tag="PC479_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2805715.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1910..2455
                     /locus_tag="PC479_003196"
     CDS             1910..2455
                     /locus_tag="PC479_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805716.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2457..2548
                     /locus_tag="PC479_003197"
                     /pseudo
     CDS             2457..2548
                     /locus_tag="PC479_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
     gene            2890..4815
                     /locus_tag="PC479_003198"
     CDS             2890..4815
                     /locus_tag="PC479_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2805717.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5216..7120
                     /locus_tag="PC479_003199"
     CDS             5216..7120
                     /locus_tag="PC479_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2805718.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3007 a          714 c         1353 g         2067 t
ORIGIN      
        1 gagtacaata tagaatttgt tatgtagtat agtagtatat tagaatacaa tatagaattt
       61 gttgggtagt atagtgttat attagaatac aatataggat ttgttatgta gaatatatag
      121 agtcatgtag aatatacata tataaaagga ttttgggtat aaatttaaaa attaatagca
      181 aaatgagtag tatagacata taattagtag taggaagtaa ttaaattttt cctaaacaaa
      241 aaaaatagtt gcaaataatt tgattaagtg atatagtaaa taaggtaaga ataaaacaaa
      301 gaagaagtcc gcttctcacc ttacgccgga ggttagtaag gtaaatataa gttaaagctt
      361 aattttaatt taagtttgtt taaatttggt aacttatatt gcggatgatt acgtcatccg
      421 ctttttattt tataaaaatt ttggaggtgt cctacaatta gcaaagaatt agcaatcaat
      481 gaacaaataa aagataagga aataagagtc ctttcaccaa caggtgaaca acttggagta
      541 atgcctacta aagaagctca agcaatggct aatagcaaaa acttagattt ggttcaaatc
      601 tcgccgaatg caaatccacc agtatgtaag ataatggatt atggaaagtt tagatatgag
      661 caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa ctatagttgt aaaagaagta
      721 agacttagac caggtattga acaaaatgat ttaaatacga aagctaataa tgctataaag
      781 ttccttaaaa aaggggataa agtcaaagtt gaacttagat ttagaggaag agaattagga
      841 cataaagaca taggtaaaga agttatgctt aagtttttag atataataaa agagtttgga
      901 gaaccaacaa aagcccctgc atttgagggt aataatatgg ttgtaattat agatccaaaa
      961 aaatagatga ttaactgaga ggaggaattt gtcatgccta aaatgaaaac tcatagaggt
     1021 gcagcaaaga gattaaagaa aacaggaact ggaaagttaa agagagctaa agcttttaaa
     1081 aaacatatat taactaaaaa atctgcaaaa actaaaatga acttaagaaa atcaacttta
     1141 gtaagtgatg gagatgctaa gagaatagca caattattac catactaaga tacagattgt
     1201 aaataataac tatagaagag ggatataaaa ggaggtcata acgatggcaa gagttaaaaa
     1261 agctatgaac gctcgtaaaa agcataagaa aatattaaaa cttgcaaaag gattcagagg
     1321 atctagaagc aaattatata gaccagcaaa tacattcgta atgaaagcat taaaaaatgc
     1381 ttatatagga agaaagttaa agaagagaga cttcagaaaa ctttggatac aaagaataaa
     1441 tgcagctgct agaatgaatg gaatatctta ttcaagatta atgaatggat taaaattatc
     1501 tggtgttgaa gttaacagaa aaatgttatc tgaaatggct atacaagacc cagaaggatt
     1561 tgcaaaatta gcagaagttg ctaaagcaaa attagcttag tattgaatca tgagatgtaa
     1621 agtctttgag ttatataaat aattcaagga cttttttatt ttttgaaaaa tacttattag
     1681 tgtcatgtac aaccaatttt ataatgataa ataaaataag caatacaata gaattttatg
     1741 agatttatta taagaattgt tacaattatt tatgatagct ataaaaagtc atagtaaaaa
     1801 tgatattgat gtatgttgat atatgtataa agtttccaat caatcttgaa ttaatattat
     1861 caacatgata atattatctt attaataata taaataaggg ggacataata tgaatgaaaa
     1921 actaatggat tgttttgtaa acccaattaa atgcaagtta ttgattgaaa ttttttcaaa
     1981 taatcgaact acagctaaac aattaggaga aaaatttcaa gatatatcaa aggcaacact
     2041 ttatcgttac cttaataaaa tgttgaaaga tgatattata aaagtggttg aagaaaataa
     2101 aataagggga actgttgaaa aagtatatgc tatgaagtta gatatagctg atgatgtgaa
     2161 caaaattata gatgagaact ctggagaaga atatgctcaa ctgtttactc aatatatgat
     2221 gggattttta agagaatttt atgaatacgc tgaaagaaag gaaaaggata tcctaaaaga
     2281 tggttcagga ttttctgtct gtccagtata tgctagtatt gatgagatag ttgaagtgag
     2341 taaaaaaatt gctagtatac tagaacctct aagaaataac tcacatgaag aaggtagaag
     2401 aatgcattct ataggagtga taattactcc acctaaaaag gatgaaggga aataaatctg
     2461 atttacatgg ggtagagcca atttatacag ggaatcgttt tatgatttac atatgtatcc
     2521 agagcaaagt atctctgctt ggattgtact tcattgtacc atttcatcca atgacttgga
     2581 gtggtacaat gaagtttttt aatttttcat attaatgcta aagaaatgaa ctttagacca
     2641 catatgcaat taggttgcat tatacaatta gaccacatat acaaaataaa aatcagtatt
     2701 tttatagcag ataaattgac tgtcattctt cttaaaaatc atctattagt tattgttagc
     2761 atattgaaag aaattatagt aatctattga ataaatgaat attgcctata atcatataaa
     2821 ctgctatagt ttaaaataaa gcaggacgtt aaattttata gtaatctaaa aatttaagag
     2881 ggggatttta tgaaaataaa atttgttatt atgccattgg tgatattttt aatggtatta
     2941 tttgtggggt gtcaaagtac aaaagataca gaagttaaag caaaagatac agaaattaaa
     3001 atattaggga caggtgattt acattcaatt ttgacagact ctatggtttc gtatgtaaat
     3061 gaggaaaggg aaaagaataa aaatctgctt atggtagatg cgggagattt ctatggtaca
     3121 caaagtagag aaatgtggga atggtcttct ggaaaaaagt taattaatat taaaagaaat
     3181 ggtagagcag agtatgatga cataattaag agtagtaaag atgaagtacc tatagttaaa
     3241 gatatggcaa agttgaaata tgatgctgta gttttgggag ataatgaatt tgtttcaaat
     3301 gataagcaaa gtcttgataa actggtttct gattttaaaa ataataatat gccattggta
     3361 tcagcaaata tatatgaaca atctggagaa aattatgtac aaccatatgt aatgaaaaat
     3421 attaagacag atgagggcaa tgtaaaagtt ggaatactag gtcttactat aaaagaagta
     3481 ggggaaaccc ttggtcttga ggattttgaa aaggataaaa aagctagaga gcttggagaa
     3541 caagctgatt acaaaggaaa attatatgca aatgatttag tagaagatgc aaaaaaatgg
     3601 ataaaagtta tggaaaaaga aagtccagac ataatagttg cagttgtaca ctcaggggaa
     3661 gaacctaaaa cagatagaaa tccaggaaat agaataaaag agttagcaac tacagtggat
     3721 ggaatagatg caattgtagc tggtcatact catgaaaaaa tagatgaaca caaatataaa
     3781 aataaatctg gagatgaagt aattgttaca caaccaggtt ggcatggaga tagagtatca
     3841 gagattaatt tcaaactaaa taaagagaat ggaaagtggg acataaagga aaagtctagt
     3901 aaattaaaaa taattgataa agaagtaagt atagttgcta ctgcagattt acattcacat
     3961 ttctctgata aattaacagt tgaccttgtt aatgaacgaa gtaacccaat tgaacctgtt
     4021 gtagtagatg caggcgactt cttagaccct caaacagatg aaatgactca atggtataaa
     4081 gagtggaaaa gtataagaga taataatagt gataaaacga ttagtagatg cccaatggtc
     4141 atggatatga atcagggaat gtatgatgca gtagtacttg gaaatcatga atttgtatct
     4201 gaaaaagatg aattttggtc agatgaacgc cttttagatg ctgttataga ggattttgaa
     4261 caggtggcaa tttctgtgtt atcggcaaat atatataggc aatctggtaa aaattatgtt
     4321 aaaccatata ttataaaaga tgtggagacg agtgaaggta atgtaaaagt aggaatattg
     4381 ggacttacta taaaagaagt aggtaaaggt cttaaaaatg taaacttaca agagcaattt
     4441 caatataagg ataaattgta tgcaaatgat ttagtggaag atgcaaagaa atgggtaaaa
     4501 gttatgaaaa agaaaaaacc agatataata gttgcagttg tacattcagg agaagaacca
     4561 aaaaatccaa aacatccagg aaatagaata aaagaactag caactacagt agatggtatt
     4621 gatgcaattg ttgctagtca tactcatgaa aaaatagatg agcatgaata taaaaataag
     4681 tctggagaaa aagttatagt gacacaacca ggtgagcatg gtaaatatta ttctaaaatt
     4741 acctttaaag taaataaaga aaagggttcg tggactatta atgataaatt cagtaaaaca
     4801 attgaggttg aataacttta taaaatttat agataaagta aattatctga tattggcatg
     4861 atatataaga taaagtttct aatattagta taatgcataa tgtgaaatat tttaataata
     4921 agagaataaa caagacaaat aaaaatattt taacatgtaa gatatagtat gaactattat
     4981 actaatgctt actttataat ttgaacatgt tagaataagt cttatttgaa aattttagtg
     5041 tactagagta agtattattt tactagtaaa aaatattgag aactatatgt agatttgctt
     5101 aaaaaggaat ttacatatag ttcttttcgt ataataaata gtacaatctt atatggtaaa
     5161 gataaagtgt gatattaaat tttattatca tccaaaatct aaggaggagt ctcttatgaa
     5221 aataaaattt attatagtag catcaataat atttttaata gtattacttg tggggtgtca
     5281 aagtactaaa gacacagaaa tcaatgtact cgccacaagt gatttacatt caattattcc
     5341 agataattta atttcctatg tagaagagga gagaaaatat gatgagaatc tacttatggt
     5401 agatgcagga gatttttttg acatgcaaag tggtgagatg aataagtggt ttactggtca
     5461 aaaattagta agatttaaag atgggatacc agagtacaaa aaaatagctg aaccacgtga
     5521 aggagaagtt ccattggcta aaaaaatggc aaagctgaag tatgatgcag ttacatttgg
     5581 gaatcatgaa tttgtatcta atgacaagca gagccttgat aaattggttt ctgattttaa
     5641 aaataacaat ataccattag tatcagcaaa cgtatatgaa caatctggag aaaactacgt
     5701 aaagccatat gtaatgaaaa atataaatac tgacaaggga actgtaaagg ttggtatact
     5761 tggtctgact ataaaagaag taggggaacg ttcaaggttt aaaagttttg aacaagataa
     5821 aaaggctaga gaacttgggg aacaacctca atacaaaggg aaattatatg caaatgattt
     5881 agtagaagat gcaaataagt gggtaaatgt tatggaaaaa aaagaaaagc cagatataat
     5941 agttgccatt gtacattcag gtgaagagcc aaaaaatcct aaaaatccag gaaatagaat
     6001 aaaagaacta gcaacaactg tagagggaat agatgctatt gtagcaggtc atacacatga
     6061 aaaaatagaa cagcatacat ataaaaataa ttctggtgaa gaagtaatag taacacagcc
     6121 tggagctcat ggtaatagta tatcaaagat taattttaag ttggataaga agaatgataa
     6181 gtggtttata aaagacaaat atagtgaatt aaaggttttt ggaaaggaag taaatataat
     6241 tacaacttct ggtctgaatc caaaattttc agatgaaatg gcagttaatt tgtttaatga
     6301 aagagataga gatgttcaac ctatctttat agatactgga gattttttaa atactaatac
     6361 aaaagaaatg actaagtggg ctaaagagtg gcaatacaat gaaaataaag gcatctataa
     6421 aaaaataaat gaatggccaa ttgtatatct tggttatgat gctgtagtac ttggtagcca
     6481 tgaatttaca gaggataaag agacttttga ggaaaaaaaa tctacattgg attatattat
     6541 agaaaacttt gaagaaatga agataccagt attatcagct aatatatatg atgaatctgg
     6601 agaaaattat gtaaaacctt atataattca taatgtagaa actagggaag gaaatataaa
     6661 ggttggagta ttaggtctta ctactactga taaggatgaa gaagacttaa gtaactcaag
     6721 cttaagcaaa gactcaaaga cttcgaaaat tcctgaagaa acaggaaagt tatatactaa
     6781 taatctagta aaagatgcaa gcgaatgggt aaaagttatg caaaaggaaa gtcctgatgt
     6841 gattgttgca gttgtacatt cagataataa tagtactcta aaaaatccaa gtagtgaagt
     6901 caaaaaactt gcaatgtcag tagatggaat agatgctatt gttgtaggtc atacgaaaaa
     6961 taaggtagaa gaacatattt acaaaaataa gtctggggat gaggttatag taacacaatc
     7021 tggagaagat tgttattcaa aaataagtct tgaactaaga aaagaaaatg gcaagtggaa
     7081 tatcataaac aaatatagta aagcaataaa aatgaagtga atttattgat atataaacca
     7141 t
//
LOCUS       DAMJYX010000137         7135 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJYX010000137 DAMJYX010000000
VERSION     DAMJYX010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.142"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(529..1935)
                     /locus_tag="PC479_003200"
     CDS             complement(529..1935)
                     /locus_tag="PC479_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2805719.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1980..2687)
                     /locus_tag="PC479_003201"
     CDS             complement(1980..2687)
                     /locus_tag="PC479_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805720.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2858..3772
                     /locus_tag="PC479_003202"
     CDS             2858..3772
                     /locus_tag="PC479_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805721.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3772..4533
                     /locus_tag="PC479_003203"
     CDS             3772..4533
                     /locus_tag="PC479_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805722.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4545..5282
                     /locus_tag="PC479_003204"
     CDS             4545..5282
                     /locus_tag="PC479_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805723.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
     gene            6160..6429
                     /locus_tag="PC479_003205"
     CDS             6160..6429
                     /locus_tag="PC479_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2805724.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(6647..6892)
                     /locus_tag="PC479_003206"
     CDS             complement(6647..6892)
                     /locus_tag="PC479_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2805725.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
BASE COUNT         2490 a         1023 c          923 g         2699 t
ORIGIN      
        1 taaaaatact acacaaaaac tctactttag tagaactcaa tggcgcaaaa tacttcaatt
       61 taaaacattt atcgtacttt aaataaataa ctataataaa atatacttac aatagttaca
      121 tagaagctat tataactaca cagaacagct tataattaaa acttttttag gatgtcatta
      181 tattttttct taaaattaaa aaacactata ccataattct attttagtag aatttttttg
      241 tcaataataa aattaaatat taactaaaaa ataaaaaaaa tggaattatc taaagaattt
      301 aataacctta caataattcc aaaatattat ttctatgaaa attttgatac catgagtaac
      361 tcagccataa gcgcataaag cacaagttac tctttacttt aataaaactt gacaagaaaa
      421 gaatttatcc ttattctact ctaatagagt taatttgtca ataaagaata aaattatttt
      481 caaaatattt tcattcttca ttcttatctg ttattatttt ataaaatatc acttaattag
      541 aggtaatttt agtattgttt ttagtccctg actatcaatg ctcgtaatat ctaatttacc
      601 tctatgtgca ttaataatgc cagatactat caaaatacct agaccatgat tttgaccttt
      661 ggcatatgcc tcacttagtc cattttggct ctttaataaa ctattaattc tttcttgact
      721 catacctttt ccatcatctt caacaattat atagcaaaaa tctaaatcag acttagaggt
      781 aacccttata ttacatcctt gttggttatg agttatacta ttttgtatta aattactaat
      841 agcccttttt aataattttt tatctgcatc tataataata tcttcattta gtatatcaag
      901 ttcaacgtaa aagtcattat ccaaattatt atttataaac tcagaaataa gctctctcaa
      961 aaacgcagaa actctaatct tatcacaatt caatgtttgc atattatatt caagtttaga
     1021 cactaaattt aggtcattta ctaattctct gattttaatc ccttgattgc atataataga
     1081 cacttgctct tgttctctat ttgaaaggct actgctttct tcaagttctc cagcatatcc
     1141 aattatcatt gaaagtggag ttcttatatc atgagatatt cctgcaatcc agttagacct
     1201 agcttcctct ttcttcctaa gtttttcatc tttcatttgc aactctttag atacagtatt
     1261 aatagatttt gaaatatcac ttagtattcc tttttcttgt aaaagtactg gttctccttt
     1321 tgacataagc tttatgccag taataattgg ttttatagat ttcaccattt ttaaacctat
     1381 gaatagagct attaaaaaag taactattgc attaaccaga aacataccta ttattaacat
     1441 aggaaattgt tctatttcct taaccgtata agtaaggtta aacttagtat acttgtcttt
     1501 tggataacct gttactaata ttccatttgg atgttcccat acatacacag gatagtcctt
     1561 taaaaagaac cttgaaaact ttgctacatc tgtcaaagta taatgacttg gaatttcttt
     1621 tggtaaatca aatccccata ttctatctcc attgttatca attaacattg cccaaacttt
     1681 atttttatgt agtacttcct tactagaatc atctaaaata aactttccat ctcttaaaat
     1741 caaattatct tcaatcataa tcaagtttga ttttatttct ctactatcac ttgtatctaa
     1801 attatcagag tatttagata caaataacat atatgaaaac agaagaaaac ctgctatatt
     1861 taatacaata aaaagtaatg aaatcaagat acttgaacct ataaatcgcc tcaatatgta
     1921 ctttacacta tccaaaatta aatcactcct cttgatttct tttattctaa tatctaaaat
     1981 taattaatca tcaacttata tccaagacct cttactgtaa taagatgttt aggtgtagat
     2041 ggagtttttt ctatcttctc tctaaccctt ctaacatgaa ccataagggt attttcatat
     2101 ccatacatat catctcccca aatggcttga catagtgaat cattagtaac aattctccct
     2161 ttattttcat acaactttaa aagcaacgaa tgctctttag ctgttaaaga aatctcttgc
     2221 ccatctttct caacacatgc actatttaaa ttcacaacaa cagaatctcc cagtttaaat
     2281 actggcaact cctcttcttc tataggcaca tatacacgat ttaatatacc cacaagcctt
     2341 aaagtaagtt cctttggtaa aaatggcttt actatataat cgtcagcccc taagcccaaa
     2401 ccaatcaatc tatcttcatc ttcacctctt gcagagagaa aaatcactgg tattttagat
     2461 gtctctctaa tttttctcat aatagaaaaa ccatctccat ctggcaatac aacatctaaa
     2521 attgcacaat ctggctttac atttctaaat accctaacag cttcctcata acttgatgct
     2581 aaaaatactc tactaaatcc ctcttttctc aggatttcat ctatcatttt taataattcc
     2641 ttgtggtcat caaccacaag tattttttta tcttttattg actccataat catcaccctc
     2701 ctttagtata ccatataccc attttcacaa ctttgcctaa atagactttt aaggttattg
     2761 taaggtaaat ttcagctaat tgaaatgtaa ccaagttatt atactgatat aagttaaaag
     2821 aatcaataag cacagattaa aaaaggggat gaaacatatg gaaatgataa ttagaacaca
     2881 aagactttca aaggaataca ataaaatatt tagagtaaag gatatagacc taagagttcc
     2941 acaaggagct gtatatggtt ttttgggtcc taatggagct ggaaaatcta ccactcttaa
     3001 aatgcttctt ggacttgcta aacccacaca aggaaatatt aatattttag gtaaagagtt
     3061 aaatgaaaaa aatagaatat caattttaaa agatataggt tctttgatag aatctccatc
     3121 atactatggt catcttacag ggcttgaaaa catgaccata atgcaaagac tcttagattt
     3181 acctaaaaaa aatgtaaatg aagcattaaa aattgtaagg ttagaaaatc aaaaaaataa
     3241 aaaagttagt caatattctt taggtatgaa gcaacgtctt ggcattgcta tggcaataat
     3301 gaagtttccc aaattattaa tacttgatga acctacaaat ggactcgacc cagcagggat
     3361 tgaggaaatt agagaattaa taaaatctct accagaaaaa tacgggatga ctgttctaat
     3421 ctctagccat cttttaagtg aaattgacca aattgccaca tcaataggaa taataaatca
     3481 tggcgaactg gtttttcaag atagcataaa agaacttcat aatagaggtc aaagtcagat
     3541 tgctatcaaa actaagaatg ttgtatctgc acaaaaacta ttaaatacta aaggatatat
     3601 gtcacttata caagatgggt acttgactat tgaaaattta aaagactcag atgtcgctaa
     3661 aataaatggt atgcttgtcc attcaaatat agatgtattt agaatagaag aacataaaaa
     3721 aagcttggaa gatatattct tagaacttac tggtaaggcg gtgactttat aatgaaatac
     3781 cttggtctag aattttataa acttaaaaga aagaagatta ttctgatgac ctttttattt
     3841 gcagctgttg aaataatgtg gtgtatcgta aattcaaata gagctattac acgtaatcca
     3901 gatatgttag gtggatttga atattcctat atgcttatga gttttgcaag tttaaatggt
     3961 ctattctttc ctatcttaat atcaataatt acatcacgta taagcgatat agaacataaa
     4021 ggagatacat ggaagctttt aaaatcttct gtgacatctt taaatagtat ttatttatct
     4081 aaatttttat gttctgctat tttggtatct atacctgtat tgatgcaagt gttatctatt
     4141 gtgttgtttg gacattttag aggggttatt gagtcaattt ctgtatcttt acttttaaaa
     4201 tacacattag gaacaattct tgtaagtatt gctatcattg cactacacct atggattgca
     4261 actgtaattc caaatcaaat gattgcaatt acatttggca tgattggtag ttttataggt
     4321 ttgacatcag gtttgtttta tcaaggtatt cgtagattgt ttatatggtc ttattaccta
     4381 gagttatcac ctttaagtta tgcttatgat aatgttttag gttcatctgt ctatagaatt
     4441 gatatgaatt tttctattac gattttagtt ttcttaattg gaatgctaat ttattatatt
     4501 ggaagaaatc atttattaaa aaaagaagta taggagggat ttaaatgtta aggaagtcag
     4561 ttttgggaga aattctaaag ttaaaaaatt cttttatatg gtatgtatta ttagctttgc
     4621 cacttataag tgtgttgatt ggttctggta atttttatct aaatcaaggc atactcaaaa
     4681 aagaatggta cagcttatgg actcaagtta gtctttttta tggggaattt ttctttccta
     4741 tcttaatcgc aatcttttgt gcatatattt gtaggcttga acatatgaat cataattgga
     4801 ataatgtaat gacactgcca gttaatttaa aaaatatctt tttatctaaa cttacagtag
     4861 tttcaatact tacagctatt acacaagttt tctttgttat cttttatata gttgcaggaa
     4921 atatgtttgg tttttctggt agtgtaccta aagaattgac tggttggatt gctaaaggtt
     4981 ggattatttc tatttcaata gcttctatac aattatacat gtctataaag attagaagtt
     5041 ttgcaactcc tattggtatt agtctttgcc tttctctatt tggtatggga ttaatagtag
     5101 caaaacctgc tgttggtata tttttccctt attctatgtt aggtagtgct atgggggtta
     5161 taaagcaatc tgctttaaat gttaatgaca ctatagttac aatatgtgtt gcatttatta
     5221 ctactatagt attttctgaa attgccaata gaaattttag aaagaatgat gttatttctt
     5281 aatggtaaat gctagatatt tttgggtttg aaatttatat tctttttagt tgaaatctat
     5341 atccatttta tttaaaaata tatatctttt atttgaattt tatatgtttt atctactcgc
     5401 tatataatag atatactcct aaaatttaaa aatatataat aatgttatta tttaaaatta
     5461 ttatgtatta tattaaaact taatgcaaaa acaattttta tattatataa tgaaacttaa
     5521 ttaaatatag gtaaaaaatc attcaatata ttaaataaat atttaacttt aataatctat
     5581 atttaattat agctataaca tagaattatc aaatatattg aatgaatatt tgactcttgg
     5641 aatgtgtaat taaattaagt ttggttattc gatggaaggc aaatttgata taaaactcca
     5701 tttaaagtgc cagataaaaa ctttatggtt atataaaagt taaaagtaat ttatagtgtt
     5761 atagaacatt caaagggatt taaagtaatc tatggtgttt tgaaatattc aaagggattt
     5821 aaactaatct atagtcttgt agaatattca aagagattta aaacaatcta taatgttgta
     5881 aaatatattc aaagggattt aaactaatct atagtcttgt agaatattca aagagattta
     5941 aagcaatcta taatgctgta aaatatattc aaggggattt gaagtaattt attgtgtttt
     6001 gaaatattca aaaagatttt tggaatagtt tttataaatt taccagtact aataatttta
     6061 tgtttaacat ttagatgacc taaaatcaac tatttgcact tttgtattca tctaaaatca
     6121 ttttataata tttttaaata atggaaagga tgtttgaata tgtttgaatc aagatgtggt
     6181 gtctgttgtg acagttgtac aaaaaaagaa caagtaaatt gcactggttg cccaactatg
     6241 gaaaaaccat tttggggtgg agaatgtaag gttaaaactt gctgtgaaag taaggaactt
     6301 aatcattgtg gtgaatgtga tactttccct tgtgatatgc ttttaaataa gggaaaagac
     6361 caaggttttg accctatggt aaatattgag caatgtagaa aatggcttga agaaacagtt
     6421 tctaattaat tgttttagaa tgctttgtta aggaatttat ttaatccttt gttaggtagt
     6481 atgtttagtc atttgttgat tagtatattt atacatttat tgatgggtat atttagccat
     6541 acaaatagat ttacataaaa tttacgacta aaaaagagtt gagtattagt ttttattatt
     6601 taaaacggat attcaactct tttttatgtc taactttttt gattttttaa tctttccaat
     6661 accaccactt taatttttct ggttctggat tttcagttga tgcaaaatat actgcacatc
     6721 tgtatacata taactgacat ctgtctatgt gctgtccacg cattaaacat tccttttcat
     6781 acatttgtgc tgggtttgca tctttaagtg atttaatagt tgtatatcct aacatgatta
     6841 aagatttttc tgttgctttc cctacccctg gaattttttg tagttctccc atacttcacc
     6901 ttctttctgg tatattcttt ttatattata cctattacca gatatttttt tattatagtt
     6961 aatatttaat atttaatttt ttattatatc aatttattta tgctctttca tatatatata
     7021 ttttaccacc tctaaatcac tgagtcactt aattacatca taatatagtt ttatacaaat
     7081 aaaatacttt atgtttaatt taatatataa aattcacctt caagaaaatt atatt
//
LOCUS       DAMJYX010000138         6949 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJYX010000138 DAMJYX010000000
VERSION     DAMJYX010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.143"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            196..1938
                     /locus_tag="PC479_003207"
     CDS             196..1938
                     /locus_tag="PC479_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HDF2805726.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2108..2296)
                     /locus_tag="PC479_003208"
     CDS             complement(2108..2296)
                     /locus_tag="PC479_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805727.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2293..2874)
                     /locus_tag="PC479_003209"
     CDS             complement(2293..2874)
                     /locus_tag="PC479_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2805728.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            3042..3860
                     /locus_tag="PC479_003210"
     CDS             3042..3860
                     /locus_tag="PC479_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2805729.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4870..5691
                     /locus_tag="PC479_003211"
     CDS             4870..5691
                     /locus_tag="PC479_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2805730.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(6001..6780)
                     /locus_tag="PC479_003212"
     CDS             complement(6001..6780)
                     /locus_tag="PC479_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2805731.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2596 a          921 c          881 g         2551 t
ORIGIN      
        1 atagctttta ctattttgta cttaaaattt atttatcatt ttaccagtat aaatttcata
       61 tacttttttg aagttaaatt ttttatatta tttataatct tatctatatc tttatgttaa
      121 attttcttaa catgcatttt ttatatttaa tatagtaaaa taattaataa ctatatccat
      181 gattggagga tttttatggt taaatccaag aagaaaatat ttgtttttct aattttaatt
      241 gcagttttta ttatatttgc atcatacata agaaattata ctaatgatac aaaaaaagat
      301 tctgttagag gaactattcc ttttgaaaaa atggaatata agcgtcctaa tatacagtca
      361 atttgcgaaa acattaacaa gtgcaacgac aaacttttaa cagctaaaac agctaaggaa
      421 caattaaatt tatttaatga agttgataaa atttatcaag atttttacag tacacttact
      481 atagcaaaaa ttagaaataa catagattca tcagacgaat tttactctaa ggaatataag
      541 tatcttatga gtaaatcagt agatgttgac atgtcataca aagactttca agatagattt
      601 gtaacttcta aattctctaa agaattagaa aaagaacttg gaaaggatac atttaattat
      661 ctaaagaaca ttggaaaact taattctaaa gaagttgaag gtttgcttaa aaaagagcaa
      721 gacctagttg taaaatatga agacttatta gctaaatcta cagtatcaat agatggtata
      781 gaggtagact ttgaagaagc cttgtcaaga ccaaatctta gccctgaaga atatgtgaaa
      841 atttattcag attacttaaa aaaatacaat cctatatttg gtaacatatt cttagaactt
      901 atacagacaa gaactgaaat agcaagtaag caaggattta agaattatat agattatgcc
      961 tatatgaacc taaacaaaga ctattctcaa gaagaagcta aaaaatttag acaagatgta
     1021 aaagattaca tagttccatt atatagagag atttcatcta aaccatctga ttcttctatt
     1081 tatattaaag tatataaaaa tcgctcattt aggaagtttg ataaagtact tgaagatata
     1141 tctccaaaac taaaagagag cttcgactat atgaaaaaat atgacttgta cgattattct
     1201 tctggaaaaa acaaatcacc tggaggatat actacttata taaataaata taaagcacct
     1261 ttcttgttta atacatggga taattctttt ttaggtgtta ctagttttgc tcatgagttt
     1321 ggtcattttt ataattacta caacagtatt aacttaaaca ataagatgca accaagtata
     1381 gatgtatgtg aagttcattc aacatcttta gaaattctat tttataaata ttttgatgat
     1441 ttttttggta aacaatctga agctataaaa aaagaacact tatcgattgt tttaaatact
     1501 attattgatg cttgtcttta tgatgaattt caagaaatta tatacaagaa tccatatatg
     1561 agtctaaatg atatcaataa attgtttttt gatttagagg aagaatatgg agtttccaat
     1621 aatatactta gagaaaaaaa tgctcctttt tggatacttg tatctcataa cttccaagtt
     1681 cctttttatt acttgagtta tggtcttgca tctgatgtat cacttcagat atggcaatta
     1741 tcacaagaag attacagaaa agctgttgat gtatatatgg attttttaaa tcaaaatact
     1801 gatgctggat ttaaagatgt ggtagaaaag gtaaatttac aattaccttt ccaagatgga
     1861 aatttagagg aaataagctc tactttatat gattattttg gtatagataa tccgcttgaa
     1921 ctaaaaaatg cttcttaatt tctaatactt tatactcact cttaagattt agtattagat
     1981 tgaaataagt gatatttttt cacacacaat taatgtttaa taaaattata tggtaaagta
     2041 tcagaaattt ttaagactct tgtctttata atttctgata ctttatttat taataatatt
     2101 attattttta tatatatata attaaaagga ttgtggaaat caataaaaat atccctgtaa
     2161 aaatagcact attcatatgg aacttaatag aacttgcatc tgttacctta ttattcttaa
     2221 tccagaaccc acaaatcata gttgaaagaa aaagtaaaac agtgataaga cttgaaccaa
     2281 tagttataat tttcatctta catcaacatc ctctccaata atattttcta ttaaaatatc
     2341 aatgaattta tctcgctcat atttattctt tatatttata ccactgtata actgaaatga
     2401 ttctgtagat aaagcagaaa tctgtattgg taaaataatt tgcagggcaa taattcttag
     2461 actcatttca tcttttttat ttccaaaaat tttacgaagc atagggacaa tatcaagttc
     2521 tgcttgcata tctccattgg ttatacattg attcaataca aaaggaagca ctttttcata
     2581 atgtaatcca gtatcacaca atttttttaa taaatttttt aaatcttcaa taggtcttag
     2641 agtatataca ctatcgtcat atagctctgc tattatattt gacatcacat ctcctatggc
     2701 agtactcaac aaattatcct ttgttttaaa atgataattt attaaaccaa ctccaacatt
     2761 tgcacgctca gcaatttttc taactgtaat ctcctcaata tcatctactt catttaaaag
     2821 attcaatgta acttcaatta tacgttctct agcatcttta tcattacttt tcataataga
     2881 cctccaaaac aattaacatt ttcagttctg aacatgttca gtataatatt tttttcatca
     2941 tatgtcaata taatttaatt ataattttag ttgactctaa cattatgata tggtttatat
     3001 taatattaag ctaaaagtac ctttataaag gagacaaaaa catgtttaag ataggcgatt
     3061 tttcaaagct aagtaaaatt agtattcgta tgttacgtca ttatgatgaa ataggacttt
     3121 taactccttc tcatacgaat aaaactaatg gatatagata ctatagtgca gaccaacttt
     3181 ctactacaaa cagaatccat gcactaaaag atatgggctt tggcttgtat tctataaaag
     3241 aaattttgac agaatataac gataaagaaa gtctcataaa atatctaaat atccaccatt
     3301 ctcaagtaaa agaacaatta gaagatacac agaaaaaact attaaaaata gaaaccacta
     3361 ttaaaagaat tggaggaaat gatattatga aaaattatga tgtaactata aagaattttg
     3421 caccaaaata tatgatgaca cttagaaggg ttataccaac ttatcaagac gaaggaatgt
     3481 tatggcatca agcattttta gagacaaaag accagaatgt tcaaattgaa cccccaaagt
     3541 attctaaagc agttttttat gatactggat ataaagaaga ttatgtagat gttgaagtac
     3601 aagttgctgt atctggaaag tataaagata ctgaacatgt taaatttaag actgttccaa
     3661 gtgtgactgc tgcaactgca attgttaatg gaaatttcaa tcaagttgca gacgcttgtg
     3721 aggctattgg taattggata tcagataata actatgatgt ggatggtcct atgtttaata
     3781 tttatcatgt aagtcctggc aacgatagta atcctgataa ttgggtaaca gaggtatgct
     3841 tcccagttaa aaagaagtaa tttttatatt aattggataa tattttaatt tgttagacaa
     3901 attactttta gtgttatgta taaataccta caatttaatt aggatattat cttaatttgt
     3961 taaacaaatt acttttagtg ttatgtataa atatctacaa tttaattagg atattatctt
     4021 aatttgtcag ataaatcact tgtgatgtta tgtttgaatg actagaattt aatcaggata
     4081 ttatcttaat ttgtcagata aatcacttgt gatgttatgt ttaaatgact agaatttgat
     4141 tgggatatta tcttaatttg tcagataaat caattgtgat gttgtgttta aatgactaga
     4201 atttgattag gatattatct taatttgtca gataaattat ttataatgtt atgtttaaat
     4261 acctacaatt taattaggat attatctcaa tttgtcagat aaatcaatta tgattttatg
     4321 tttaaatgac tagaatttga ttaggatatt atcttaattt gtcagataaa tcaattataa
     4381 gattatatac tatccttata ctctagttaa atatatttta atcactaata taaattagtt
     4441 tttttaacaa aacaataaaa gagtaaagta caaataattc ctttgtactc tactcttttt
     4501 aactttcttt gtacaataat acaatgataa atataaaaaa ttatatattc cattctatag
     4561 aaatatgctc accaataaca tgtatgttgt taatcaatat attatatcaa attatatcaa
     4621 aattacataa accttatagg acaatcatat cttattacaa gaaaaatgtc ttatccatat
     4681 tgaataagtc attcagttca tatacaaagt caatttcatt gagaaacatt taatatatgt
     4741 ttataaaaat ttcatactat tctttcatat ttacatgtga tataatttat taactcataa
     4801 ttttgttaat cagttgtgct tgtatattat ttatataatc taatattttt agaaggtggt
     4861 gtaataactt tgaaattaaa aattgttggt agtggcggta tgtcaattat tccaagttca
     4921 ttttgtaaat gcaaaatatg tgaagaagcc aggcaaaaag gcggaagata tgaacgatta
     4981 ggaccaagtt tatatattga tgatataaaa atgctgatag acacacctga agacattgca
     5041 gtagcttgta acagacaaaa aatatcggaa gtaaaacata tttcaatttc tcaccatgac
     5101 ccagaccatg ttaagggtat tagaattgtc gaaaaaatag gatgtgactt tatcacagga
     5161 gaaaataatc caattggctt ttatgcgctt cctgaagtaa ttgaagatat aaaccgaacg
     5221 aatctcgatt gtcttaaata ttatggtgat gttataaatt gcatttctat taatgaaact
     5281 tcgcatgtaa agataaataa ttttgatatt gatttgatta acaataaccc agacaggaat
     5341 ataaccttct atgttatcaa agagaatgaa aaaaaagtca tatatgcctg ctgtaatccc
     5401 aagccattta ctcatagtga tatgtacttt gatgctgata ttttaataat aagtctagtt
     5461 tctgatgatg gtatattaaa tgatggtaca aaacttaaag acacaccatt taaagatgaa
     5521 atatttactt tagatgagat tgttgaaatt aaaaactatt atagaattaa aaaaattatt
     5581 gttacacata ttgacgaaat gtggggaaaa tcatatggtt attacagtga attagaaaag
     5641 aaacttgata acatttattt tgcatatgat ggtatggaaa ttatagtcta aatcattaag
     5701 taataagcat aatgcaaaat aagaaaactt caatttaaca gtatagtcaa actatgtgat
     5761 atcaacatat cgcagttttt acaaaatatc atagtattta tctttataaa atttcaagtc
     5821 ctacatattt taatacttca atatctaaat aaagtaaaat caaaaatata ttttatatac
     5881 attatagacg gcttatcgtc actagataag ccgttttgtc acaattatta tttgctcaac
     5941 aaaacttctc ataatatata acagattgaa tctccaactg atacttaatt accaaaactc
     6001 ttattcctta atatgttgtt gcattaacca aaccttcatc atcatttttt gtggcaatag
     6061 ttttgctaat aaatgactgg attttgtatt tataccatac acagaaatat ccttattttt
     6121 ctttgcatcc ttcaatgctt ttttggcaac aacgtctgga gacactatgc ctgggaaatt
     6181 ttttgtccct ttttcagcta caactatacc tctcttaaat agatttgtac tcatccaacc
     6241 tggacataca gcaattgcat ttatcccttt actcttaagt tcaacattta atgctcttgt
     6301 ataatttctt ataaacgctt tagttgaact ataaatattt tgatatggca atggctggaa
     6361 tgatgcttgt gaagaaatat tcatgatatg gcttcccttt tgcatatatg gaatacatat
     6421 aagtcccaat gctacaacag cactaatatt taaatcaatc atattaatag attcatctat
     6481 acttaaatca ttgtatgaac agaattttgc aaaaccagca ttgtttataa ggtattttat
     6541 acatacattt tctttactta aaaatgtacc aatttctttt acttcttcta tttttgataa
     6601 atcttttgaa aaaattttta ccttacttcc aaattcatct actaattgat ttaatctcgc
     6661 cttgtttctt gcaactgccc atatttctgt tatttcttct tcatttatta ataactttag
     6721 aaactcttta ccaaaaccac tattagcacc cgttataatt gcaatcttct tataactcat
     6781 aattattctc ctttaccata agtttatacg ctcaaactgt aaacttattt tttacaaata
     6841 aaacaccctc tattcagatg ttatattcat attatttgag atattatatt tatatcattt
     6901 tcaataattg ttatatagca taaaaaatag tgttaatatt atagaatcg
//
LOCUS       DAMJYX010000139         6896 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJYX010000139 DAMJYX010000000
VERSION     DAMJYX010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.144"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            6..2183
                     /locus_tag="PC479_003213"
     CDS             6..2183
                     /locus_tag="PC479_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2805732.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2197..2619
                     /locus_tag="PC479_003214"
     CDS             2197..2619
                     /locus_tag="PC479_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2805733.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            2793..3368
                     /locus_tag="PC479_003215"
     CDS             2793..3368
                     /locus_tag="PC479_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2805734.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(3493..4209)
                     /locus_tag="PC479_003216"
     CDS             complement(3493..4209)
                     /locus_tag="PC479_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2805735.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            4374..5099
                     /locus_tag="PC479_003217"
     CDS             4374..5099
                     /locus_tag="PC479_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2805736.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5159..6751
                     /gene="hcp"
                     /locus_tag="PC479_003218"
                     /gene_synonym="priS"
     CDS             5159..6751
                     /gene="hcp"
                     /locus_tag="PC479_003218"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2805737.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
BASE COUNT         2696 a          781 c         1242 g         2177 t
ORIGIN      
        1 aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa aatgttaata
       61 tggatagtta taaacaactt aaagctaaaa taccctgtaa ggaaacaggt atagaaataa
      121 agaggacttt atgtgatatt tgttcgccag gagtacattg tggaattgat gcatatgtta
      181 aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat aaagggttgt
      241 tatgtaccaa aggtctttgc aatagagaat atatctatag agaagataga ataaaaacac
      301 ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca tgggatgaag
      361 cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga gcaaagcatg
      421 tagctttttt tacgggttat ggaaagtggt atagaccttt tttacaaagg tttgcatctt
      481 catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca gcacagatgg
      541 ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat gcaaaaactt
      601 atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg aatctaataa
      661 aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca ccgacaactc
      721 aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct ttagctcttg
      781 gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata gaaaagcatg
      841 tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat actgtaagta
      901 gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg tcacaaggtc
      961 cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga tttcaaaatt
     1021 atagagcaat gatgtcttta tccgctataa caggtaatta tgatagaata ggtggaaaca
     1081 taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta gaaaaagagt
     1141 ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa agatttcctc
     1201 tatggaatga aatggtgact caatttcaag ctatggattt acctaggcaa atagaagaaa
     1261 agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga atgtttccta
     1321 attcagggag actttttaaa gcgttgaata gtgtagattt ttttgtgaat atagatttat
     1381 ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcatgtaca tcatttgaga
     1441 gagaagaatg taagagttat ccaggagggt atattacata taccaaaaaa gttattgata
     1501 aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat gctatgaata
     1561 tagatgatga tttattaaaa gcaggttatg aagcaagtgt aagacatatg tttaaaaata
     1621 catgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt aaagatttta
     1681 agccatatat agtaggttca tatacaagag aagggtatga tactagcact tctaggtttg
     1741 aattaaaatc tacaattatt gaaaaatata aagattttgg gctggatgca ctaccaacat
     1801 atagaggatt taatgataat atagatgatg aatatactca tttattgaca tcaggagcaa
     1861 gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga atattaaaaa
     1921 atgaagctaa ttgtgatatt tctagcttag atgcaaaaga attgggtgtt atcagtggag
     1981 attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat gttactgata
     2041 gagtcttata taagaatata catatgtatc atggatatag agaagctgat ataaatagta
     2101 ttatttgtga taattttgac ccttatagtg gattccctgc gtatagaagt tgtaagtgta
     2161 agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt taaatttaat
     2221 aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca aaatgataat
     2281 aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg tgaaaatata
     2341 gaatattttt cattagcatg tatacattgt aatgagccaa aatgtttagg tgtatgtcct
     2401 aaaaattgtt ttaaaaaaga agaaggattc attgtattag acaatcaaaa ctgtataggg
     2461 tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa tgatggtaag
     2521 gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc accttgtgta
     2581 atggtatgcc ctacaggagc attaaaattt aataaataat tgtattaaat tgctatacta
     2641 cttttaaata attgtgttaa gaattatttt ttgatatact tttgtaatat aaaatgtaaa
     2701 taaaacaaat acaaagtata taaaatatat ttttagaata aagtgtgctt tagattaaat
     2761 ttagaaaaat aaattagaca ggagaactta atatggtaaa agtattagcc tttatgggca
     2821 gtccaagaaa aaaagggaat gtagacacaa tacttgatga gataataaaa ggtgtaaatg
     2881 ataataatgt tgaagttaaa aaatattatc taaatgatat gaatattaaa ggatgtcaag
     2941 gctgtctata ctgtagaaaa gttcctactt gtgcatttaa agacgatatg attgacatat
     3001 atgaagatat aaaaagtgct gaatatataa taattggttc gccagtatat atatgtcagg
     3061 tatcagctca aacaaagcta cttttagata gattgtatcc tttaacagag ataaataagt
     3121 ctaaacatat acctaggttt ggtcaaaaaa agttaatcat ggtctatact caagcagctc
     3181 ctatttcgtt tttatttaaa aaatatttta agtatactgc aaaacattta aagggcatgg
     3241 gattaaatca ctacaaaact atagttgtaa ccaaagcatt tgaaagaaat tccactaaaa
     3301 aaaattttaa ggtattgaaa aaagcctata aacttggaat gaaaatagcc attgatatca
     3361 atggatagat aaaagtttaa tttatagtat agctaaagga gcattaatta ttaatactcc
     3421 ttttttgtat aaaggtttct agaaaataac taataaaaaa caatattatt tgaatacaga
     3481 tattatacaa gcctagatat ctaacttgtt ttgaaaataa aagatttcaa atttattttt
     3541 gttaaaagat aacaagcctt catctcgcat cttgccaagc tctcttgaca tagaacttct
     3601 gtcaacacat aaaaaatcag ccatttcatt tcttgaaaag ggaattgtaa actggtggct
     3661 acctgtttgt tctatctgtt ttgaaaaata cataagcaat ttctcgcgag ttgttttacg
     3721 agaaagtaat tctattttat tgtgaagtaa aatgtttttt tcagctatta attgtagcat
     3781 attttcaaca agcctattat gaaatacaca tgcagatgaa caaataccag ttacactttt
     3841 aaactttata aataaaactt cgcaagaaga tgttgtggtt actgtaacat tacttttatg
     3901 tagtctagta catgcgaata cctctccaaa taaatctcca ggcaagagtt cagctacaat
     3961 attcctattc ccttcgatat cttcctttat tatatgagca cttcctttaa gcataattcc
     4021 aatagaagta acttcacttc caacagagaa tattatacta tcttttgggt aagtcttatc
     4081 tacaacacct aaacattcca acatttcttc aagttcatca cttttaattc cttcaaatag
     4141 aggaattttt ttaagtaagt ctatatgctt ttctaaaaat tttttcaaat aatcacttcc
     4201 ttgttgcata tgcaaccgat aatttgtttt cattgtacta tactaagttt ataaaagcaa
     4261 ggggatataa aaatcctact acaagaaatt tgaaataaag aaatataggt attaaaaata
     4321 aatatataag aaatcaatac tttaaattaa aaaaatttta tgaggtgata taaatgatga
     4381 gaaaaataat taaaattgat gaggaaaagt gtaatggttg tggactatgt gttgaagctt
     4441 gtcatgaaga agctatagga atggtaaatg gaaaagcaaa acttttaaga gatgactatt
     4501 gtgatgggct gggagattgt ctgccaactt gtccaacaaa tgcgatttca tttgagtata
     4561 gagaagcagc agaatatgat gaagctgcag taaaagcaaa tatggaagca aagaaagcac
     4621 aaaagaaaac tttagcatgt ggatgtccag gttctcagtc taagtcaata aatagagagg
     4681 tttcaaatag tacgtctata agcaatgata tagtagagat aaaaggttct caacttaatc
     4741 aatggccagt acaaataaaa ttagtaccaa ctaatgcatc atatttaaag aatgcaagtt
     4801 ttttaatagc agcagactgt acagcgtatg catatggaaa ttttcataat aaatttatga
     4861 aaaataaggt tacattaatc ggatgcccta aacttgatga agttgactat gctgaaaaac
     4921 ttacagagat tttaaaagaa aatgatatta aaaatatagt agtaacacgt atggaagttc
     4981 cttgctgtgg tggtatagta aatgctgtta agacagctct acaaaatagt ggaaaaatga
     5041 taccatggca aatagttact atatctgtag atggaaaaat tgtagatgga gaaatttaat
     5101 taagtgaatt atagaaaaat ataataaatt ttaataaata aaaagggagg aataaaaaat
     5161 ggaaaaaatg ttttgttttc aatgtgaaca aacagcagga ggtaaaggtt gtactataaa
     5221 tggtgtctgt ggtaaaaagg ggggtacagc taacttacaa gacgaactta caggagtgtt
     5281 gattggtctt tcaagagcaa ctattggaaa taagaataga ccaacaagtg aaacagataa
     5341 aattatgata gaggggctat tcactactat aacgaatgta agttttgatg atgaagctat
     5401 aaaaagacaa attgaaaaaa ctgaggtaga aaaaagtaaa cttgtaccta gatgttcaga
     5461 ttgttcaaca acttgtaata gaaatgataa ctatgatatg aaggatttat ggaatgataa
     5521 tgaggacata cgttcattaa aatcattaat cttatttgga ttaagaggta tggctgccta
     5581 tgcatatcat gcaatggtac ttggttatac agacaaagac gtaaatgaat ttttctatga
     5641 aggattaata gcagttggag gagacttgtc tattgacgaa cttttaggtc tagttatgaa
     5701 aactggagaa ataaacttaa aatgtatgga attgcttgat agagcaaata ctgaaactta
     5761 tggaacacca gaaccgactc aggtatctat gaaaatcgag aaagggccat ttattgtagt
     5821 tacaggacat gacctttatg atttacacaa attattagaa caaacgaaag ataagggtat
     5881 aaatatatat actcatggtg agttattacc agcacatgca tatccaaaac taaaagaata
     5941 taaacatcta aaaggtaatt ttggtacagc atggcaaaac cagcaaaaag aatttgataa
     6001 tattcctgct ccaatattat ttacaacaaa ctgtttaatg cctgttaaag atagttatag
     6061 agatagagta tttactacag aagtagttgc ttatccaggt atggttcata ttgatgaaga
     6121 taaagatttt tctagtgtaa tagaaaaagc tcttgaactt ggaggatata gagaagatgt
     6181 tcatatgact ggtattaatg gaggagatac attaactact ggttttgcaa gaggtacagt
     6241 ttcatctgta caagataaag tagtagatgc tgtaaagagt ggagctataa gacacttttt
     6301 attggttgga ggttgtgatg gtgcaaaacc tggaagaaat tattacactg aatttgtaaa
     6361 acaagctcct aaagacactg ttatattgac acttgcatgt ggaaaatata gatttaatga
     6421 tttagacctt ggggatatag gtggcttacc tcgtttaatg gatatgggac aatgtaatga
     6481 tgcttatagt gcaattaaag tggcagtttc attggcaaat gcttttgaat gtggagtaaa
     6541 tgatttgcct cttagcatgg tactgtcatg gtatgagcag aaagctgttt gtatacttct
     6601 tacactactt catttaggta tcaaaaatat ctatataggg ccttctttgc cagcattctt
     6661 atctaagaat gtattagata tattggttga taaatttgca ttgacaccta tatctactcc
     6721 tgaagaagat atgaagaaga ttttaggata aataaacata tctatactta aagatattta
     6781 tagttaaaat atttttaagt atgccacaat taagataagg ggttaaactt aaaatttgat
     6841 taaaaaatca attttgagat gaccctttat ttattttttg taataattga aaaata
//
LOCUS       DAMJYX010000140         6711 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJYX010000140 DAMJYX010000000
VERSION     DAMJYX010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.145"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     misc_binding    378..616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            680..2599
                     /gene="thrS"
                     /locus_tag="PC479_003219"
     CDS             680..2599
                     /gene="thrS"
                     /locus_tag="PC479_003219"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2805738.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3367..4458
                     /locus_tag="PC479_003220"
     CDS             3367..4458
                     /locus_tag="PC479_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2805739.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            5128..6468
                     /locus_tag="PC479_003221"
     CDS             5128..6468
                     /locus_tag="PC479_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2805740.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         2666 a          607 c         1147 g         2291 t
ORIGIN      
        1 taattgtatt tatttatttg attgtgttac ttattaatag tatttaagta tttactgtac
       61 tacttattaa ccatattaag tattgattgt atttaagtat acaaaaccta aaaagagtac
      121 aaaaattaga agttagtttt aataaaaatt agttttagta tttatctagt catatctatc
      181 atgcatgtgg atacgtttta aaaattttaa atttatgaaa atattataat ataactataa
      241 atcaagagat taatttaata taaaaatata agaatattgg tttacagctg attgagatga
      301 aaaataatgg gtaattaata tattacatta atgttgattg atgtataact atctgttata
      361 atgaaaatat attaaaaatc atagtgttga agaggaagag tagagtagaa accattctca
      421 gagaatttgg gtctatggct gagagcccat gtaataggtc tatttgaaaa ctaccttgga
      481 gctgagctta ttagcagtat ttgtatttat actatacgtg aaagtgctcg ggtgacgacc
      541 ttatagtcat caagagggtt gtatatactt gtatacgcca acttgggtgg aaccgcgaat
      601 taatatcgtc ccaaacatat ttggggcgat tttttattac aaaaaatcta aaaattattt
      661 caataaggag gaaaaaacaa tgattaaagt tgctttaaaa gatggctcaa taaaagaatt
      721 tgaaaatgct atttctgtta tggatgtagc taaatccatt agtgaaggat tagctagaaa
      781 tgtggtagca gcgtctgtga acggagaggt tgtaggtctg gaccacatta tagatactga
      841 ttgtgacttg aatttattta agtttgaaga taaagaaggt aaggaagttt ttagacatac
      901 atcagcgcat atattagctc aagctataaa aagactttac ccagaagcaa agcttgctat
      961 aggaccaagt atagaaaatg gattctacta tgatatagat ttagaccata gattagttcc
     1021 agaggaccta gagaaaatag aagctgaaat gaaaaaaata gctaaagaag atttaaaaat
     1081 agaaagattt gaacttccta gaaatgaagc tttagagctt atgaaagaac aaggagaaga
     1141 ctataaggta gaacttattt ctgatttacc tgaaagtgaa ataatatctt tctacaagca
     1201 aggtgatttt actgatttat gtagaggacc acatttacca tctactaaaa aggtaaaagc
     1261 tgttaaatta caaagtgtag caggtgcata ttggcgtggt gacgaaaata ataagatgct
     1321 tcaaagaata tatggaactt cttttgagaa aaacaaagac ttagaagagt acttacattt
     1381 actagaagaa gctaaaaaga gagaccacag aaaattaggt aaagaattag gattatttat
     1441 gatacctgaa gaaggtccag gattccctat gtttttacca aaaggtatgg aacttaaaaa
     1501 tgaattatta aaattctgga gagaaataca cagaaaagct ggatatattg agatagaatc
     1561 tccaatcata ttaaatagaa aattatggga gacttcagga cactggtatc attacaaaga
     1621 aaatatgtat acagttaaaa tagatgatga ggattatgct ataaaaccta tgaactgtcc
     1681 tggtggatta atttactata actctcaact acattcatat agagatttcc caatgagagt
     1741 tgcagagctt ggtagagttc atagacatga gttatctggt gcattacaag gattaatgag
     1801 agttagagca tttactcaag acgattcaca tatattcatg ttaccagagc aaattaagga
     1861 cgaaataaaa ggagtagcta acttaataga tggtatatac aaaacttttg gatttgaata
     1921 taatttagaa ttatctacaa gaccagagaa ctctatggga agcgacgaag agtgggaagc
     1981 agcagaaaat ggattaagag aagctttaga agagttaggt ctaccatata ctattaatga
     2041 gggtgatgga gcattctatg gtcctaagat agacttccat ctaaaagatt gtttaggaag
     2101 aacttggcaa tgtggtacta tccaattaga tatgcagctt ccaagacaat ttgataatac
     2161 ttatattggt caagatggtg aaaagcatag accagttatg attcataggg ttgcttttgg
     2221 tagtatagaa agatttatag gtatattaat agagcattat gctggtaaat tcccagtttg
     2281 gttatctcca actcaagtta agatacttcc tatatctgat aaatttatgg attatgcaaa
     2341 tgaagttaag aaagagttat ttgataaggg tataagagtt gaattagatg atagagctga
     2401 aaaaattgga tttaagataa gagaagctca gcttgaaaaa gtgccataca tgttaatagt
     2461 aggtgaaaaa gaagtagctg ataataatgt ttctgttaga tcaagagata aaggtgaaat
     2521 aggtagtatt aaattagatg aatttatagc tagtatttca aaagaaattg aatcaagaga
     2581 aagtattatt caagactaat atatattata ttagtagcta agatattgtt atataagata
     2641 agtttaatca atatgttgat atgttgatgc atagtgatta aatttatgaa aagatgagta
     2701 taattagagg tacagttaat catattttta tgattaatgt acctctagtt ttttgtatgg
     2761 tagatttatt aataaatata tcatagaagt gttttgaaca tggaaatatt tatgtgcatt
     2821 ctttaaataa aagtttattt tttgagtgat aattaagtgc ttgttggtaa ggttatggct
     2881 gtaaaaatca taactttatt ttcatatttg ttccattttc ataaaaatta ttatcccttt
     2941 aagaaaattt cttaatcctc ttgaaatatt tcctttttag tctaaagtta aatttctttc
     3001 ttcaaaatga atatttaaat gaactaaaat ttaagtacat tttctaatct tatgagtcta
     3061 tttttcatag ataattgccc tataatagaa gaatctatgt tggtgctatg tggtgacaat
     3121 aacagcattg tcagtgatta attaatgctt tataaatgat taattagtgt tttatgttat
     3181 ttttggactt atcctctata catagattaa tttatttaac tttagataga gagagaatgt
     3241 caagtacata cacagacatt agagtttaag aaagtgttta tggagcaaga acttttaaaa
     3301 gaggtagtga cttagtttaa tttcaagaaa tgataaatac aaaagaggaa aaagagaggt
     3361 gttgaaatga tagaaaaagt agaagaaaaa tctaagatta ttaaaacatc aaaagaatat
     3421 aagcgaacta taattgaaga aaaagataaa gaatggacca aaaaattaaa tgatatactt
     3481 agtagaagta gtttttctaa tgatgaatta gataaaatca aggcattaat tccaaaagag
     3541 attttgacta atgaaaatgt tggagaagtt gtgactgaag atggatatgc taagcctgag
     3601 tatgatgagg tatttaaatc attgttcact ttaaataatg aatatgattt acttgtaaat
     3661 tttataaatg atattctaaa agatgctcca tatgctaaca ggaatatcaa accgttcact
     3721 caaataaaga gaataattag agttgaaact gatccaacaa ttaattacat tggagagaaa
     3781 agacctaaac ttgatatttt ggctgaagat gaagaaaata atcatataaa tattgagatg
     3841 caaagagctt ttgagaacga ttatttggaa agagctgaat attatcttag tcgtgtacat
     3901 ggtagaaagt tagaagaagg taaagagtat aaagaaattg gaaaaactat tggaatacat
     3961 atactaaatc atgtaaaata taatcacata gaagattatg taaattgcct gagattaaca
     4021 atggacgggc atccagatat ttattctagt aaaactgcat tatattttat agagcttcca
     4081 aaaattcata aatctgacta tatagtaaat agaattatgc tatggggaaa acttattagt
     4141 aatccttcta acttagatgt aagagttata gctaagaatg attctattat taaaaaagca
     4201 cttgatagat taaaagaatt aggtagcaat gaagaatatt tattaaatct gaaaagaggt
     4261 gcttatatca tgaataaaag tagaaatttt aaagaagaga ttgaaagaga gacagcaatt
     4321 agagttgcaa aagaaaaaga ttataaaata attataatgt tgaaaaaaaa tggttttaaa
     4381 ttagatgaca tattaaataa atttaatgat tttgacttaa gtgaagatga gataaaagaa
     4441 gtatatgaaa ataattgatg ataaataaaa agtgaactta tataagtttg ctgaatcgga
     4501 gtctggaaat atttatatgt attttttaat gaatgaaaat ttattttaaa aacaataatt
     4561 tagaagtaca gttaattata tttttatgat actgtacttc tagttttttc tattgtagat
     4621 ttattattta ctaataaatc tacaatagaa atgcttttgt tgtaagaatc attttatcta
     4681 atgattgttt tttataatag ttaaatttcc atttgtagtg tttaaatata aatctataac
     4741 agcatttcta taaatgttat aaatcactat aatgattcaa ctcaaagtta ctgcttaaag
     4801 aagcagagat tttatcaagg tatatcatgg aaaaaaatat tttattgtaa tatttataag
     4861 attttattat agaaataatg taaaaacttt ataaataata tattttatac ctagttatag
     4921 ataaatttat atagttatta ttaaaaaaag caataaaaag tttaattcaa tttagcaaat
     4981 ttagtgatat aattaaaaaa gaaaagaaat atggacttat ttggaataat tttgacaatc
     5041 tatttactac atgtgtagag tcgtatataa ttaaattata cataaaatat aaaaaaataa
     5101 tttagtaata cgttaggaga aagtattatg ataaagtcaa gcttattttg gaatattttc
     5161 attgcgatat cctttatgat agaatggttt ctttgtaaga aaatgctaga ttatacaagt
     5221 gagaaaagga tttctaacaa aaaaataaac ttttgtatgg taggtattat tgtttttgct
     5281 gacataatat atttttttga tgtacctcca aatataagag taattatatc tatgataatt
     5341 actattgcat tttataaaat ctcatatgat gtggggttta taaagtctgt attaatatcc
     5401 ttaatttatt ggatgttact aatcgccaca gatgctttga gtatatcttt aacagtttgg
     5461 cttaattcta taaatgacat gagtagtatg gggacgggag atatatacag aattgagtct
     5521 ataatattgg gaaagatact tttgattgtg tctttgcttt tatatagagc cttaaagata
     5581 aaacttaata tagaaataaa taaaaggaca ttattttata tagttattcc tatagtcgct
     5641 aatatagcat ctttttttgt aatttttgaa tacatattta atccagggtc tggtagctta
     5701 atacataatt tccaattcgt tatgatttct gttgtattgc ttatatcaag tatcacttta
     5761 gttttaattg ctaaaaaatt acagcaatat aatactttag ttgtagaaaa caatatgatt
     5821 aaaaatactt tgaagtatga aaatgagtat tatgaagata tagaaaagaa atatttaaaa
     5881 actagattac taagtcatga tatgaaaaat cacatattgt gtataaatgc tatgattaaa
     5941 aaaggtattg atgttacttc ttatttaaat aatttgcagg tagaaattca gagtaatgac
     6001 ttgttattta atactggtaa ttttatatta gatgctatat taagtgaaaa gaaagaggtt
     6061 tgtgataaga atgatataga actaaaaata ggaataaatt ttagttgttg tgaagctgtt
     6121 gaattagtgg atatatgtca tatattttca aatattttag acaatgcaat tgaggcatgt
     6181 aataaaattg atagtggaaa tagagttatt tgtgttaaag gtgatgttat gcaaaatttt
     6241 tatttaataa gagttgaaaa tactaagata aataagatta ttactaaaaa tggagatatt
     6301 ttgacagaca aaaaaggttc tcttcatgga cttggaataa aaagtgtaaa aaactctgta
     6361 aataagtatg gtggtaatgt tgtaattgaa tatgatgata aaaaatttgt agttaaaatt
     6421 gctattccac ttagaaatgt gaagaaagca gttaatcatg tgatttaaat gtatataaat
     6481 ttgatgtgta gaaatttaat atataagaat taatgtataa aagtgatgca tagaagttta
     6541 atatataaga attaatgtat aaaagtgatg catagaagtt taatatataa gaactaatgt
     6601 ataaaagtga tgcataaaag tttaatatat aagaatttaa tatgtataaa agtgatgcat
     6661 aaaagtttaa tatataggaa tttaatatgt ataaaagtga tgcatagaaa t
//
LOCUS       DAMJYX010000141         6630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJYX010000141 DAMJYX010000000
VERSION     DAMJYX010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.146"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(114..257)
                     /gene="scfA"
                     /locus_tag="PC479_003222"
     CDS             complement(114..257)
                     /gene="scfA"
                     /locus_tag="PC479_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2805741.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(323..685)
                     /locus_tag="PC479_003223"
     CDS             complement(323..685)
                     /locus_tag="PC479_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2805742.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(743..1036)
                     /gene="yajC"
                     /locus_tag="PC479_003224"
     CDS             complement(743..1036)
                     /gene="yajC"
                     /locus_tag="PC479_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2805743.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1170..2291)
                     /gene="tgt"
                     /locus_tag="PC479_003225"
     CDS             complement(1170..2291)
                     /gene="tgt"
                     /locus_tag="PC479_003225"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2805744.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2370..2855)
                     /locus_tag="PC479_003226"
     CDS             complement(2370..2855)
                     /locus_tag="PC479_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805745.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2867..3892)
                     /gene="queA"
                     /locus_tag="PC479_003227"
     CDS             complement(2867..3892)
                     /gene="queA"
                     /locus_tag="PC479_003227"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2805746.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4210..5229)
                     /gene="ruvB"
                     /locus_tag="PC479_003228"
     CDS             complement(4210..5229)
                     /gene="ruvB"
                     /locus_tag="PC479_003228"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2805747.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5336..5938)
                     /gene="ruvA"
                     /locus_tag="PC479_003229"
     CDS             complement(5336..5938)
                     /gene="ruvA"
                     /locus_tag="PC479_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2805748.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5931..6440)
                     /gene="ruvC"
                     /locus_tag="PC479_003230"
     CDS             complement(5931..6440)
                     /gene="ruvC"
                     /locus_tag="PC479_003230"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2805749.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
BASE COUNT         2041 a         1280 c          747 g         2562 t
ORIGIN      
        1 cataaataga atatatattt ttatataata attgaaaata aaagaggata cctcataatg
       61 ataaaagaat cactttgaga taccctctta caaacaacta aatcaataat tttctatctt
      121 tcacactctt gatttgcaac tgtacaagaa gttttacaag ctgattgaca agaagtttga
      181 cactctccac atccaccttt tgcagcactt tgctttaaag tagcttgaga taaagttttt
      241 acatgtttat tttccatttt caacgcctcc atttctaatt gtttcctatt gattatatca
      301 taaaactact ttaatataaa ctctatttaa agttgacacc caatacgcct ccaatgcctc
      361 ctgctgctga tattaacaat aacttataaa tcatccctac ttcaaataca aacttttctt
      421 gtgctaaaaa cacagtcaaa aaaatacata ctatgtacaa aagtcctact aaaagcccat
      481 acataagtcc tttttctttc atgtgttttg aagtgtaaaa ccctcctata gctgcagaaa
      541 tagtagttat aaatgatgag accatagtta tattatctcc accaatatct gtaaatgtca
      601 aaaataagtt atacactaat aaaacagcca aagttattat atatgcatat cctagacctt
      661 taaaaatata attagatttt cccataaaaa aacctcctct caaattaata tatattcgag
      721 aaaaggcttt taaattacta atttagtctt cttctatttc ttctttttta gatttaacag
      781 ttcctatagc ccatttttca aatggaactt tagttctatt tggtcctaat tctaatatta
      841 caacatcatc ttcaacttta gctacattag ctactattcc acctattgta atgaccttgt
      901 ctcctacact taggctactt ctcatttctt ttaattgttt gtcttttttc ttttgaggtc
      961 taatcattag gaaataaaag attgcgatta ctactaccca tagacctata ctcataatta
     1021 tttgttgctg tggcattaat ttttcactcc tttgtaaatt atactctttt gtaaatcata
     1081 ataaatgttt atataacaat ttactttctc tatctttgag tactattgaa tattatatat
     1141 taaaatgatt ataattaata gtgcaaaacc tatatttcat atccatattt tgcaaaaaat
     1201 tcatttctaa aatcaagtaa tctatcttcc attatcgctt gtcttatttg tttcattaaa
     1261 tttaataaaa agtgaagatt atgagtagtt attaatcttg cacccagtat ttcatttgct
     1321 tttataaggt gtcttatata agctcttgaa tagtttttac aagcataaca atcacattca
     1381 gggtcaagtg gagtaaagtc ttcagcataa gttgcatttc taactactac tttaccttgg
     1441 ctagtcattg cagttccatt tctagcgata cgagtaggta ggacacaatc aaacatgtct
     1501 actcctctta taacaccttc tactaagtca tctggactac caactcccat taggtatctt
     1561 ggtttatcct ttggcataag tggagttgta tgctctaaca cgtcatacat aagtggtttt
     1621 ggttctccaa cactaagtcc accaatagca tatcctggta aatcaatatt agttatttct
     1681 ttcgctgatt gttcccttaa gtctttatac atcccacctt gtattatacc aaataaagct
     1741 tgcttgtcgg tattattatg ggcatcttta catcttttta accatcttgt tgttctttct
     1801 aaagaatttt ttacatattc tctatctgat gggtatggtg cacactcatc aaatgccatc
     1861 attatgtctg aacccaatgc attttgtatt tccatagctt tttctggagt tagaaaatgt
     1921 tttgaaccat ctaagtgtga cctaaattct acaccttctt cttttatctt tcttaaaggc
     1981 cctagactaa atacctggaa ccctccacta tctgtaagta ttggtttatc ccaattcata
     2041 aacttatgaa gaccaccagc tctttttata agttcatgac ctggtctcat atataaatgg
     2101 tatgtgttac taagtattat ttgtgaacca atctctttta attcttctgg tgtcatagat
     2161 tttacagttg cttgagttcc aactggcatg aatattggtg tttcaataat cccatgtgga
     2221 gtgtgtaatc ttcctaacct tgcaccactc tgcttacaag tctttatcaa ttcgtatctt
     2281 actgcgtaca taaagttctc cttaatcata tttatttctc attatttctt aagatatatt
     2341 tgtaataagc tattcatata atttattgtc taaatagatt tatcttcgtc ttctttgttc
     2401 tgttgttcct taattgcttc ttcatcatga atactcatta tattaagagc actcttgtct
     2461 tttgttacta aaaacttttt tatagcaaat agtcctgcta tagctataac tcctattaaa
     2521 acatctatca taccttcatt tttaggcagt agcaacattt ttctagcaat tgcgtaaagt
     2581 aatgcctcaa taaatgctcc tggtatatga agagatagca ttactacaag ttcaacacct
     2641 attactaaaa gtaatatttg tgctaaaaaa tcattaagct gactgtattg tactggattt
     2701 tgaaaatcaa ctatatatgc tccccacata agtcttagaa catctatagt tcctaaaaat
     2761 actgctatta gaactactac tgctaaaaca cgttcaaata taaaagcaat ctttaatata
     2821 gggttattct ttttttgttt ttgtaaacta tgcatctatt acctccctac tttataatca
     2881 tagcatctcc aaaactgaag aatctatatc tttcttttac tgcctcatta tatgcattta
     2941 aaacattatc ctttccacaa atagcactta ctaacattat tagtgtagac tctggtaagt
     3001 gaaaattagt aataagttta tctaaaactt taaatttata acctggatat ataaatattt
     3061 ctgtccatcc actagtttct ttcatctttc catcctcatt acaagctgat tctatagttc
     3121 tacaactagt agtaccaaca catattacat tttttccatt ttcctttgct ctatttattt
     3181 tgtcagctgc ttcttggcta accatataat attctgaatg catcttgtga tttaaaacat
     3241 cctctacttt tactggtcta aaagttccta agccaacatg tagagttaca aaggctatat
     3301 ctacaccttt ttcttttatt ttatttaaca attcttctgt aaaatgtaat cctgctgtag
     3361 gtgctgctgc tgaaccatta tgctttgaat aaacagtctg gtatctttct ttttcatcta
     3421 gtctagcagt gatatatgga ggtagtggca tatttccaag ttcatccaat atttcttcaa
     3481 aaattccatc ataatggaat tttataattc ttgaaccttc atctgataaa tctacaactt
     3541 ctccaattag ctttccattt ccaaaagaaa atttagtccc tacttttgct ctttttcctg
     3601 gttttactaa agcctgccaa gtatcttcct ctgttctttt taataataaa aactctattt
     3661 tacctccagt ttcaagcttc tctcctatta atctagctgg tataactcta gtattattta
     3721 atactaagca atctcctgga tttaaatatt ctattatatc tctaaatact ttatgttcaa
     3781 tattacctgt ctctttatct aataccatca atcttgagct agctctatct tctataggaa
     3841 cttgagctat aagttcctgt ggtaagtcaa atttaaaatc acttgttttc aatgttaaac
     3901 ttcctctctt aaaatttctc cagctacaat aatatcaaaa aaacaactta ttttccacaa
     3961 tattaccata atctatcaag aaattgctat ttttaacaga caccatatcc acaatatata
     4021 gatatttttc cacgatatac atgccttatc cacaatatat agatacaata cacacaattt
     4081 ttatcgtgtt tatccacagt atctacagat ataattacca ttttgtggat atgtttgaat
     4141 ttttagccat tttagaaata ttttaattta atgactcttt tattttataa atactcgtat
     4201 aatctatttt tatttttcat tgggatatgg aatttttaag tgttcatatg ctaatggcat
     4261 agctactctt cctctaggac ctctatttat aaatcctaat tgaagcaaat atggctcata
     4321 tacatcttct attgtatttc tatcttctcc gatagaagct gccaatgtat ctaaacccac
     4381 tggtcctcct ctaaattttt ctattatagt cataagaagc ttctcatcta caaaatcgag
     4441 acccaaacta tcgactccta aaagttccag agcatctttt gccactttat cagttatttt
     4501 tccatcagct ctgacttgtg caaaatctct aactcttttt agtaatctat ttgctatcct
     4561 tggagtccct cttgaacgtt ttgcaagttc taatgctcca ttagactgta tctctgcatc
     4621 taagatgctt gaagacctaa gtactatttt tgaaagttca tcaactgtat aatagtccaa
     4681 tttacaaata acaccaaatc tatctctaag tggatttgta agcataccag ctcttgtagt
     4741 agctccaata agtgtaaatt taggaaggtc aagtcttatg cttctagcag atggcccttt
     4801 ccctattatt atatctaggc aaaaatcctc cattgctgga tataaaactt cttcaacact
     4861 tctattgatt ctatgaatct catctataaa taaaacatca ttttcattta aattcgtaag
     4921 tatggctgcc aaatctccag ctctttctat tgcaggtcct gacgtaattc ttaaatttac
     4981 ccccatctca ttggctatta tacttgctag tgtagttttt ccaagacctg gtggtccata
     5041 taaaagcaca tggtctaact gctcatttct cgatttagct gcttctataa atatactaag
     5101 ttgttctttg gatttttctt gacccaaata atcttctaaa gtttttggtc ttaaactgtt
     5161 ttctatatct atatcttcca tcttcattgt agaagttatt atcctatttt catcttcaaa
     5221 accttgcatt tatacccctc ctctcgtact tttgaaaatc tactttatat attttcaaat
     5281 ttacataaac tccttaaata ttgttttaaa atccaaatat tataaaatcc aaatactatt
     5341 tcaaggattt actcattata tatgtaagtg ctttctttat aatagcttct gtattcatac
     5401 catctttttt acatttttga actgcctctt ttgattccga caaagtatag cctaaagcta
     5461 caagtgcatc tacagactca tcttgtgata tcaaggtagg tttttgtgat aataacgttg
     5521 gttcaaactc tatgttattt ttgtctacct tatccttaag ctctaagacg attctttctg
     5581 ctgtcttttt accaacacca ggagctttgg ataattttcc aatgtcttca cttaaaatat
     5641 atgctcctaa ctgtgctggt gatgcaaatg atagtattga aagcgccacc ttaggtccta
     5701 ttttagatac agatgttagt aattcaaaca tcttaagttc ttccctactt acaaaaccac
     5761 aaagactcat atcatcttct cttacaatta atttagtata tatttttgtg gcttctccca
     5821 cttgtaaatt cattattgta ttggatgata catttatttt atatcctatc ccattatttt
     5881 caacaactat actgtctata taaatttctt ctactgtacc ttttatataa ctatacatac
     5941 ttaccaccta tattctttag agttttttca agtttgtttg aatgagcatg gcaaatagcc
     6001 acagctaaag catctgcaac atcatctggt tttggaactt tttttagact taaaaatgaa
     6061 gttaccatct gttgaacttg tgccttatct gctctcccat atccaactac accttgtttt
     6121 acttgaagtg gagtgtattc atatacaggc ttcccattat gagcacatgc aagcatagta
     6181 acacctctag cttgtgcaac tgttatagct gtttttacat tcttgttaaa gaataattct
     6241 tccattccaa cttcatctat attgtaattc tttactattg tatcaattcc tttatacaca
     6301 agttccaatc ttctcgatat attcatatgg gcaggtgttg taactgctcc ataatcgatt
     6361 gctttaaact tgctattttt gtattcaatt ataccatatc caactatggc tatacctggg
     6421 tcaatcccca atattatcat tttttaaaat tccccctaaa aataaaagaa ataccaaaat
     6481 gtatttctca taaaaactta tgttctaaat cattatatca taaataatat taacaaatca
     6541 taaaatgctc tatgtaaaat ataaaatcaa ataaaaaagt tatatcaaag caagatttta
     6601 cctcaatata acttttatat ttattacaaa
//
LOCUS       DAMJYX010000142         6584 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJYX010000142 DAMJYX010000000
VERSION     DAMJYX010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.147"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(619..1179)
                     /locus_tag="PC479_003231"
     CDS             complement(619..1179)
                     /locus_tag="PC479_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2805750.1"
                     /translation="MLKKLKKMLLITCCLFMMSSTAFAETQNSEGKSFTISGYTFMYE
                     NAPQEIKDKYKENCLDIGVEPQPNDEIFITPSELKTYSNIDMYSARSSSFTVTYHYSY
                     YKVTGDKNYTVNTSTIVKYGNSGNPVHLVQLLLKKVGYALEVDSQFGNTTLSKVKSFQ
                     GSYGLTVDGIVGSATWKKFASATNLD"
     gene            complement(1225..2208)
                     /locus_tag="PC479_003232"
     CDS             complement(1225..2208)
                     /locus_tag="PC479_003232"
                     /inference="COORDINATES: protein motif:HMM:NF017390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2805751.1"
                     /translation="MSNLILTDILRTSITSSLVICFLLLLRTTIFRIFNKGFNYYIWL
                     IVIVRLLFPFSYFTYTISDSKFQYKPILNEVDYSNTVLIIYLTIVILLAIYRFIKYLK
                     FKSLVIDLSYDIENLEINNLYNNLLKELNIEKHIKLKYTYEINTPSFFGLFDNYILLP
                     PHNYNLNELHWILKHELIHYKSKDLYIRYLVLFLKCVYFFNPFIYLLDRIIYHSCELH
                     CDEVVLKGCSLENIKSYANTILDSIERSSRDSNNYTTGLHRKSNFEKRVYDMFKNKGK
                     SGILIALILCLLSSFTYFKFDSIISKPINHSLDAPLPVNTGKEKSFTITID"
     gene            complement(2221..2583)
                     /locus_tag="PC479_003233"
     CDS             complement(2221..2583)
                     /locus_tag="PC479_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2805752.1"
                     /translation="MIEKIPRAELKVMMLFWDTDKVLTSRDIVNIMSEEWKQTTTLTL
                     LSRLSKRGFLEPTKKPTHTTYKILIDKKSYLEFATSDFVKNVHNDSIVSLVDSVNKEK
                     IGKDELASLRAFIKKLKK"
     gene            3826..4473
                     /locus_tag="PC479_003234"
     CDS             3826..4473
                     /locus_tag="PC479_003234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805753.1"
                     /translation="MNKKLEKPFVYKREYDLTGYDVEILQKYELEQAIYVYVGSSCAY
                     NMRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNIINDKLYYSNIAEF
                     EEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNITKNGNTNYLKVK
                     NKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSRPSRVFLK"
     gene            4748..6385
                     /locus_tag="PC479_003235"
     CDS             4748..6385
                     /locus_tag="PC479_003235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805754.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNS
                     KQLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINR
                     YALGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVS
                     EVTNTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKK
                     YCKDVKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQ
                     VISVAREWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIY
                     SDEDTLFNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITY
                     KRKKKVKNIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYT
                     ISYFVTMAVIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQ
                     QRISNMIESLLDTKILDVWETKINDRGFMYYTYRLNK"
BASE COUNT         2683 a          692 c          917 g         2292 t
ORIGIN      
        1 ctaattagta ggtgcttttt taaaacaagt taaaaatcaa aaatagtatt agtaagcatt
       61 ggaaatgcta gattctaaaa tagaaaagta aaaaattggt gcactatcta aacttatcta
      121 tatcgctttt tccgtcgttt ggttctctag ttacgataca ggggatatgc ttatattgag
      181 ttatagtact aatcagtgct taatatagtt aataaaatta tagttaccat agtttagtaa
      241 ctatgatgta tgttagttag aaacttgcat ttcatgagtt gtaatatctt aaagtcagta
      301 gttttgattt taaaatatta attgcactgt tacaaatgac tttattaata aaaaattagg
      361 cagcttaaaa aaagtttaga ataaatttgc taggctttgc taagctattc ttttaaaaat
      421 aactttacaa aacaaacaaa taaggactta taaatataga taaaaaaaga aaattgaaaa
      481 ggattataga gacttacaag gctttaaata tatattctta attgtaaaat tatgtaatta
      541 aaatatttac tttattataa aaagacatga taaacttatt atcatgtctt ttgttaaact
      601 tatttatatg taaaaattct aatcaaggtt tgtagcagat gcaaattttt tccatgttgc
      661 actaccaaca attccatcta ccgttaagcc ataactgcct tgaaaacttt taactttgct
      721 aagtgtagtg ttcccaaatt gactgtctac ttctaaagca tatccaactt tttttaatag
      781 caattgtact aaatgaacag gatttcctga atttccgtat tttacaatcg tagaagtgtt
      841 gacagtatag tttttatctc cagtaacttt gtagtatgaa taatgataag ttactgtaaa
      901 tgaacttgac ctagctgaat acatgtctat gttgctataa gtttttaatt cactaggggt
      961 aataaaaatt tcatcattag gttgtggctc tacacctata tccaaacaat tttccttata
     1021 tttatctttt atttcttgtg gtgcattttc atacataaat gtgtaacccg aaatagtaaa
     1081 gctttttcct tcactatttt gtgtttctgc aaaagctgtg ctactcatca taaataaaca
     1141 acaagtaatt agcaacattt tttttaattt ttttaacata attcctccct ataattttga
     1201 atatatttga ataccagcaa atatttaatc tattgttata gtaaaagatt tttctttgcc
     1261 tgtattaact ggcaatggtg catctaagga gtgattaatt ggctttgata taattgaatc
     1321 aaatttaaaa tatgtaaatg atgacaataa gcataaaatc aatgctatta aaataccact
     1381 cttaccctta tttttaaaca tgtcataaac tcttttttca aaatttgatt ttctgtgtaa
     1441 tccagtagtg tagttatttg aatctcttga acttctttca attgaatcta aaattgtgtt
     1501 agcataactt tttatatttt caagggaaca accttttaat acaacttcat cacaatgtaa
     1561 ttcacagcta tgatatataa ttctatcaag tagataaata aaagggttaa agaaataaac
     1621 acactttaaa aataaaacca aatatctaat gtataaatct ttacttttgt aatgaattaa
     1681 ttcatgtttt agaatccaat gcagttcatt taaattataa ttgtgtggtg gcaaaagtat
     1741 ataattatca aataatccaa aaaatgaggg cgtattgatt tcataagtat attttagttt
     1801 aatgtgcttt tctatattta gttcttttaa taaattatta taaagattgt ttatttcaag
     1861 attttcaatg tcatatgata agtcaataac caaactctta aactttaaat attttataaa
     1921 cctataaatt gcaagtaaaa taacaattgt tagatatatt attaatactg tatttgaata
     1981 atcaacttca ttcaaaatag gtttgtattg aaatttagaa tcagaaatag tataagtaaa
     2041 ataagagaat ggaaaaagta atctaactat tacaattagc caaatataat aattaaaacc
     2101 cttattgaaa attctgaata tagttgtcct taaaagtagc agaaaacaaa taactagact
     2161 acttgtgata ctggttctta aaatatctgt tagaattaaa ttagacatat atagtaccta
     2221 ttatttctta agttttttta taaaagctct caaagatgcc aattcatctt ttcctatctt
     2281 ttctttatta actgaatcaa ctaaacttac aatagaatca ttatgtacat tctttacaaa
     2341 atcagatgtt gcaaattcta agtagctctt tttatcaatc aaaattttat aagtagtgtg
     2401 tgtaggtttt tttgtaggct ctaaaaaccc tcttttagaa agcctagata atagtgtaag
     2461 tgttgtcgtt tgtttccatt cttcactcat tatatttaca atatctcttg atgtcaatac
     2521 cttgtcagta tcccaaaata gcatcattac tttaagttca gcacgtggta ttttttctat
     2581 cataatatgt tcctccatcc taatgttaaa aaaattaata aatgtaattt tattctattg
     2641 aaatagagta ggacagtcaa ttgttttttg attaatgatt tacatatgta gagtaaatta
     2701 aaagaagtat atcacttgaa aagttttaca tacgtagagc atttttatca aatctcttaa
     2761 tttttttaat tgaattatat atgtgaatat aagagtaaat caacttaaaa catgcaaaaa
     2821 attaatattt actgacatgc aactgacaaa tcaacaaaaa taggaattga taaaataagt
     2881 gtatatattg aaattgctat aatacataca aaaatggcat aaaatactaa tttttatgtc
     2941 cttttctact gacatgtaaa aaagtcttgt aagtattgga attactagat tacataggtt
     3001 ttagatgcaa aattgtcgtt ttgtaaaaaa ggctactaaa atttataaaa acaattatta
     3061 attgatataa attatcacaa aaaaagacat ctatttctat gaaaaaaaaa ttttcgtctg
     3121 catgtaaaaa tagctatagt aatgcgtacg aatgttctat tattacagaa ttatttgtat
     3181 gtaaattata ttgaaaatat ctatcagaat tagaatttta tatggataaa acagaactat
     3241 caaataggag tatatagtaa agatttatat aatatgatga cctcgtacaa gcctgtaaga
     3301 gccgtttaaa agttgcttta gtattaatta tctttgaaaa tttaaaatag gtttaaatgg
     3361 cttgttaaat atgatgtatt actattatgt ataggggggt agttatgtag ttacatgttg
     3421 ttttaaaaaa taaatttaac tatagttctt tcacttccac attacaaaaa ataaattgag
     3481 tgtgtatgtt tttaagtaaa aatgatgttt aaatattgaa aaatagagat atagtgtatg
     3541 taaaatttag aatagtgtat gtaattttag gcataaaaat atgtgattaa accttaaaaa
     3601 ccaggaatat agtgtatgca tttttaaaaa taaaaattgt aacctttttg taatctttta
     3661 ttaaaatact tactttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga
     3721 agatgttata atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat
     3781 aaatagattt ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta
     3841 gaaaaaccat ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta
     3901 caaaaatatg agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac
     3961 atgagagcta gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt
     4021 tgtaacatta aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg
     4081 tcagataata ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt
     4141 gaaacactag aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta
     4201 gattctataa atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg
     4261 ttaaacataa ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa
     4321 aaacataaga acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga
     4381 agtgtcaaaa gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc
     4441 ctaagccgac caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat
     4501 aatactacta gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa
     4561 tatcggtcta actgattaag tatctgtagt tatataataa tattgctatc aattttagca
     4621 tcttaacaat attattatac atactaagct aaaattattc aatagttgta aaagttgatt
     4681 agtcaataag tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa
     4741 taaatatatg gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt
     4801 tagagatggt ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca
     4861 aaataataaa agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa
     4921 aacaactttt tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta
     4981 ttatgaaaat acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa
     5041 cttaataaat agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt
     5101 taattataaa gatataatta atttatttac taagatagga ttacattatc atatcctagt
     5161 tgatagtgga aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt
     5221 agtatcagaa gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt
     5281 atctactcaa gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt
     5341 aaaaataata caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa
     5401 atattgcaaa gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa
     5461 gctaccaaat tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct
     5521 agatttgcaa aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat
     5581 atccgttgct agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga
     5641 atatcaagtc aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg
     5701 tgagtttaat agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga
     5761 tactttgttc aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg
     5821 ggttaaaata atgactggta atcaattgtt tatctataat gtgttactta acaataaaga
     5881 tagagaatta aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt
     5941 taaaaacatt gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa
     6001 tgattatatt acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt
     6061 aaaagaagtt agatgtaata tagataaaca atatactatt agttactttg ttaccatggc
     6121 agtaatttgg ggaataattt caactgaaga attaagatta tatactcata tgagatataa
     6181 gcaagattta ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga
     6241 ggaattagca aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt
     6301 attagatact aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta
     6361 ctatacatat agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct
     6421 atttttatgc aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa
     6481 ttgcaaaaaa aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg
     6541 taaaataatt gtacatatat atgtagtata tatatatatg tttt
//
LOCUS       DAMJYX010000143         6465 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJYX010000143 DAMJYX010000000
VERSION     DAMJYX010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.148"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     misc_binding    100..311
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            474..1763
                     /locus_tag="PC479_003236"
     CDS             474..1763
                     /locus_tag="PC479_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2805755.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1817..2896
                     /locus_tag="PC479_003237"
     CDS             1817..2896
                     /locus_tag="PC479_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2805756.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2917..4461
                     /locus_tag="PC479_003238"
     CDS             2917..4461
                     /locus_tag="PC479_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2805757.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4624..5166)
                     /locus_tag="PC479_003239"
     CDS             complement(4624..5166)
                     /locus_tag="PC479_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2805758.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5314..5598)
                     /locus_tag="PC479_003240"
     CDS             complement(5314..5598)
                     /locus_tag="PC479_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2805759.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5661..6425)
                     /locus_tag="PC479_003241"
     CDS             complement(5661..6425)
                     /locus_tag="PC479_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2805760.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2246 a          805 c         1124 g         2290 t
ORIGIN      
        1 catataaaac taaaaaatgc aagtaattga ctttttaagt tgacaaaata ttgcatatat
       61 attatgatat aaatcataaa ttaactacaa atcaatatta tatattcgta gaagaagaaa
      121 gtagcataaa ggaattttac agagagttag atttggctga gaactaatat tggaatttat
      181 gtgaaaagga ctttggagaa tactattgta attatgttag aatagtggtt taattcgcct
      241 taagaaaatg agtgagtaaa attaattttt actaataaga gtggtaacac ggatttatat
      301 tcgtctcttg gatttttcca agaggcgttt tttattataa acttatttca gaaaatttaa
      361 aactatatat taaatgaaaa ttataaaaat atgatagtta ataaatttat aaattagaaa
      421 attgtaaaat atgctaaggt cagtagtttt acgatttaaa tggagggatg aaaatggaag
      481 aagaaaaaat taactatgct ctggaacaga ttccagagaa tcaaaaacgt ggttgggtag
      541 ctatgttttc agttttagta gcaataggtg tggatttatc atctgttatt ttaggcgctg
      601 agttggcaca gagtatgcca atgaaacaag caatattgtc agtaattgtt ggttcatttt
      661 tttctgcaat tttgtatact acatgctctt tggttggttc atctacaagt ttatctacat
      721 ctatgataac aaagtatgta tttggagaag ctggagcaaa aatattttct ctagttatag
      781 gggtttctct attaggttgg tttggagttc aagtaggttt ctttgctcaa aatgcacaga
      841 taataataaa agatatattt aatttagatg tcagtatgca gattttaagc ctaataggtg
      901 gtctcttgat gatgagtact gcaatttatg gatataaggc aatggagaag ttgagtgtgt
      961 attcagtacc atttttatta gttcttatga tgctaactat attcttagct tttagagcaa
     1021 atggaatttc agttgatgat aatatgaaat ctactatgac atttgcaggt ggagtttctc
     1081 tgtcaatgag tataataata gttggtgcaa ttgtatctcc tgatataagt cgttgggcta
     1141 aaagtagaag agactgtgct ctttcaagct ttttgggaat acaatttgga aatgctttta
     1201 tgataatagt ttcgatagta ttagtaaaat gtatgggaac ttcagatatt atgagaattt
     1261 ttattacatt aggaattgct ataccaggta taatagtact tacattagca caatggacta
     1321 caaatacaag taatgtatat tcagcatcat taagtatagc tcttgtactt aagaaggctc
     1381 ctgagaaagt attaactata gtccttggta ttatagcaac attattggct gtatttggaa
     1441 tatatgaagg ttttatagga tttttaaatt tacttggaat tgtaattgca cctgttggtg
     1501 gtgtatatac agcagaatat tacatagtaa agcaagaact taaaggcttt gataaaggag
     1561 tattatataa accaatagtt aaacgttcaa tagtgtcttg gataattggt atattaataa
     1621 cttacctttc aacatatgga tttataactc ttactactat agcaccttta gatggtttta
     1681 tagcagggtt tgtagtacaa tctattattg gaaaagtgct ttgttcaaca aaaaataagc
     1741 aagaaataga caaagcagta taaagaaatt tgtttataaa gtataaaata aattagaaat
     1801 atgaggagaa agtaacatga gatttttaac agaagaaagt gtagataaaa tagcagttgg
     1861 tgcagcagtg cttggaactg gaggtggagg tgacccatat gtaggtaaat tggttgcaaa
     1921 acaagccatt aaaaagtatg gtcctgtaaa agtaataagt ttagatgaac ttgatgatga
     1981 tgctttggta gttccagtat ctggtatggg ttctcctgta attacaatag aaaaattact
     2041 ttcagaagta gagttaacta cccctttaga aataatggag aaattattaa atagaaaagt
     2101 ggatgttata attcctatag aaataggagg gataaactca cttatgccta tagcagttgc
     2161 tgctaaaaaa ggacttccta tcttagatgc agattctatg ggtagagctt tccctgaagc
     2221 tcaaatggta actttttatc ttgaaggata tgaggcatct cctgccgtaa tgtctgatga
     2281 aaaaggtaat tctgccatcc tttatcctgt ggatggaatt tggtcagaaa gattagctag
     2341 aactttaact gtagaaatgg gaggaagttc ttctattagt gactataatt taagtggagc
     2401 tcaagttaaa aaagctgcta taggtaatac tcttacaata gctgaaacta taggagggtt
     2461 tttacttgaa aataaatctg attcaaaagg tgctgttgag aatatactaa aagaattgtg
     2521 tggatacaag ttatttgaag gaaaagtaat tgatattaag agagagttga aaggtggatt
     2581 tactagagga catgcctttt ttgctgggat taatgagtat gatggagagt attctatatt
     2641 attccaaaat gagaatttaa ttgccaaaaa aggtgataca ccactttgta ttacaccaga
     2701 tttaatagca gtattagatt tagaaactgg atttccaata actacagaga gaataaaata
     2761 tggctctcgt gttatggtag tagcttttcc ttgtaatgaa aaatggagaa cagaaaaagg
     2821 aattgagaca gttggacctg gatactttgg atatgatgtg gaatataaaa cagtagaaga
     2881 attacaagga aaataaaagt ttaggaggaa attttgatgt atcgtgtagg aattgacgtt
     2941 ggaggaacaa atacagatgc ttgtatactg gatggggaat taaaagtaat tcattcagtt
     3001 aaggtggcaa ctacaaaaga tgttgaaact ggtgtttaca atgccttaaa aaaagttata
     3061 gatgaaagta agattgacca ttcattaata aaatatgcta tgcttggtac aactcattgt
     3121 acaaatgcta ttgtagaaag aaaaagactt aataatgtgg ctatgataag aataggaaag
     3181 ccggccacta ctgccattct tccatttata gattggccag aagacttaag agaaaaaata
     3241 gaattagatt caatattggt atctggtgga tatgagtttg atggcagaaa aataaatgaa
     3301 ctttgtaaag atgaagttgt agagttttgc aatagaataa aaggcaaagt ggaaagtgtt
     3361 gcaataagtg gagtatttgc tccagtaaat aaacaacaag aagaagaagt tgcaaaatgg
     3421 gttagagaaa ttttagggga tattcctata tctttatcaa atgaaatagg taatataggt
     3481 atattagaaa gagaaaatgg agctatttta aattctgcat tgtctcaagt aggtaaagct
     3541 gtatctttag gatttgaaaa agcacttaaa gatttaggta tagatgctca aatatatttc
     3601 agtcaaaatg atggaacatt aatgaattta gagtatacaa tgaaataccc aatttttaca
     3661 attggatgtg gaataacaaa ttctataaga ggagcttcct tcttatcaaa agtaaaaaat
     3721 gcaatagtat tagatgttgg tggaactaca agcgacttgg gtgtactata taatggattc
     3781 cctagagaat catctattcc agttacaatt ggaggagtac atacaaactt tagaatgcca
     3841 gatgttttaa ctattggtct tgctggtggt acatgtgtaa aaggcgaaaa agataatata
     3901 aagataggtc ctgaaagtgt gggatataga ataactgaag atgcaataat atttggtgga
     3961 gacactctaa ctttatcaga tgttgttaca aaattagata tggcgtttga agatagaaaa
     4021 agcaatgttg aaaacttaga taaagagtat tgtaatgagg tatataagaa ggttatttta
     4081 aaattagaag atgctgtaga ccaaatgaag actagtcaag atgatgttga attggtttta
     4141 actggtggag gaagtataat tgttcctgaa aaactaaaag gcataagcaa tgttataaga
     4201 cctcctcatt atactgctgc aaatgctatt ggtgcagctt taggacaaat aagtggagat
     4261 gtagaaaaag tatacagttt agataaaatg tcgagagaag atgccataga agatgcaaaa
     4321 aaagaggctg taaataaagc tctaaaagct ggtgctaaag aagatactat agaagtatta
     4381 aatatagaag atgttccact tgcatacctt cctggtaatg cacttcttat aaaagtaaaa
     4441 gttgttggtg atttaattta aagaataaat ataagtattt ccagaattat ataaatattt
     4501 ctggactcat ttttaaactt attttatcaa taaaaacagc ataacttttg actatattct
     4561 tataagtttc cgaacagccg atatatacct attttttgag gtttatcggc tgtttgttat
     4621 cttttatcgc attccaacta tttttcttac cgtatttgct gtacgaattg taatcttatc
     4681 acttacattt gaactggcag tctttgacca ccaatttgtc tttttataat ctttacgact
     4741 aaaagaccaa aagatagcat ttttataatg atatactttt tcatactctt ctttaggatt
     4801 tccaacctca tcataaaact cgtcataaga taagtcagga aacataaaaa taagattatc
     4861 atatatttct ttgttgttat ctccccacca attaggagca ttatttagta tatcttttaa
     4921 ctcttcttgt gaaataataa aaactggtat ttccaattca aactgttttt ttatcattaa
     4981 tcttattgta tttgagataa ccttgctgtc atctacatca cttaaaaaga caacattacc
     5041 actattaagg tatgtagaaa cttctgaata accaagttct acaaagcttg cttttaattc
     5101 agacataatt atcttgttct taccacttat attgatacct cttaataatg cgatataact
     5161 tttcatataa taatttcacc tctaaacatt cacatttttc tatgtagtac aaacttgaat
     5221 ttgcatcttt tataattctt tcaaaacttc ttcatatgta atttttttaa tataaaaaaa
     5281 tcggtgctta atttgtaccg atttttgttt ttcctagtaa agaactcgtt tttttgcttc
     5341 tgctatagct ctagcagcac tttttgcttg cattcttttt tcttcttctt ttttaaattc
     5401 atttattgtt tcatttagat tcttatcatg ttcatttttt tctttatctt cgttttctct
     5461 gtatactaat atataatcta ttattgattc gtttattatc tgagatatac ttttattttc
     5521 attaattcct attctcttaa cttccttaag tagagtctca tcaatatgaa ccgttgtttg
     5581 ctttgtattt tttgccacct taatcacctc ttaaaaaaaa ttctaacatt aaaatttttt
     5641 ttagtcaata aattttaata ttaaaattct ttaaatgttt tatttgattt tctaaaattt
     5701 tctattgtat ttatcatctt attttttata ttttcaccta gtacagaaga ctttagtcta
     5761 ggattttcga tatggtcata aattggttct aaaaattcaa cttcgacagg aacagagtta
     5821 attttaccaa taggttggta tccttcataa gtatcttttg aattttttat tacgagtgga
     5881 acaatagggc attttgcttt atatgctatc tttaaagctc cacttctaaa ctccgaaacc
     5941 aatgaatttg gttctttaat ccatgttaaa tctccttctg gaaacactgc catactttga
     6001 cctgtaagta tattttgaga ggcttgggct atactcttta taccctcacg attgtttttt
     6061 ctgtttacat atacacatct tagtaatttt gcccattctt taaatatagg tatatttctc
     6121 caaactggtt catctgcaat tacacatcca attggtcttt ctacacttgc tgttagtatg
     6181 aagctgtcaa gcatactaga atgatttaca acaaatagaa gtggttcttt tggcagtgtt
     6241 tccttaccaa ttatattaag ctctatattc actatatcta aagatttttt agcttgtttt
     6301 tgcataaatt caaatttttc ctttaagcta aatttatcag gattcttttt aattttaata
     6361 agcctaggta tagaagatgt aaaacctata agttgatatg tagcaaattt taagtaatta
     6421 ttcataaaat tatctcctta aaataaaatt atttattact gtaaa
//
LOCUS       DAMJYX010000144         6420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJYX010000144 DAMJYX010000000
VERSION     DAMJYX010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.149"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..410
                     /locus_tag="PC479_003242"
     CDS             <1..410
                     /locus_tag="PC479_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HDF2805761.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /locus_tag="PC479_003243"
     CDS             complement(710..1297)
                     /locus_tag="PC479_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2805762.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="PC479_003244"
     CDS             complement(1383..1820)
                     /locus_tag="PC479_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2805763.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="PC479_003245"
     CDS             1924..2922
                     /locus_tag="PC479_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2805764.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="PC479_003246"
     CDS             complement(3042..3968)
                     /locus_tag="PC479_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2805765.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="PC479_003247"
     CDS             4353..5957
                     /locus_tag="PC479_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2805766.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         2397 a          792 c          978 g         2253 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagttttata
     6001 taataagtta ggtagaatta aataagatta cagttaaatc aacaattata tttttattaa
     6061 attttaatta ttgtattgaa gatttataag tataatttta ttgattctac ctttttaaat
     6121 aactgtcgat ttattaaagt tatctatgta ctatattaaa caattaagta gttattaatt
     6181 gaacttatta gtttattaaa aaactatgta tattactttg cagtaagact ttggaactaa
     6241 atctataata gcaaatttaa atttattaaa tagattacct tataacattt catataaaat
     6301 atattgtgaa tctgagaaaa ctactatttt tctttgagta ttttttgtaa gcagttctaa
     6361 aagattcaat ttcataccta ttacttgaat cattcattaa aataattcta atttaaaaaa
//
LOCUS       DAMJYX010000145         6383 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJYX010000145 DAMJYX010000000
VERSION     DAMJYX010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.150"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            361..1305
                     /locus_tag="PC479_003248"
     CDS             361..1305
                     /locus_tag="PC479_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2805767.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1327..1953
                     /locus_tag="PC479_003249"
     CDS             1327..1953
                     /locus_tag="PC479_003249"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2805768.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1987..3036
                     /gene="hisC"
                     /locus_tag="PC479_003250"
     CDS             1987..3036
                     /gene="hisC"
                     /locus_tag="PC479_003250"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2805769.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            3039..3617
                     /gene="hisB"
                     /locus_tag="PC479_003251"
     CDS             3039..3617
                     /gene="hisB"
                     /locus_tag="PC479_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2805770.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3619..4224
                     /gene="hisH"
                     /locus_tag="PC479_003252"
     CDS             3619..4224
                     /gene="hisH"
                     /locus_tag="PC479_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2805771.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4221..4943
                     /gene="hisA"
                     /locus_tag="PC479_003253"
     CDS             4221..4943
                     /gene="hisA"
                     /locus_tag="PC479_003253"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2805772.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4925..5683
                     /gene="hisF"
                     /locus_tag="PC479_003254"
     CDS             4925..5683
                     /gene="hisF"
                     /locus_tag="PC479_003254"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2805773.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5711..6379
                     /locus_tag="PC479_003255"
     CDS             5711..6379
                     /locus_tag="PC479_003255"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2805774.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2610 a          540 c         1254 g         1979 t
ORIGIN      
        1 attcaatttt ttaaataact attgccaatt tttgaaaaat atattattat taatagtaac
       61 aaaaaactgt aaatttagaa tattaataaa aattatttgg gaggaaatta tcatggtgag
      121 attaaatata gtaaaattaa atacaaaatt aaatcatcac catcatcata gattaaaggg
      181 attgtaatat gaaattaatt catagacaca agccctgttt ttagtgtaat tggtcttgta
      241 tctatgatgg gaagtaattt taagagaaaa ttatagccat ataggtataa agcctatgtg
      301 gcttttttat tgttttaaat ttgcaaatat aagagtaaat ttaatgaaga gggtgagaat
      361 gtgaaatttt tgaggattga agatgaggtt atttattcta aaaaaagata taaattaaaa
      421 agggaaatag ataaactttt tttggatgaa gaatattttc aaatagaacc acaattattt
      481 gaagaatatg atgaatttac aactataaat aataaaattc cagaagaatc tatggtaaag
      541 gttgtaaatg gaaaagtaat ggtacttaga gctgatataa ctacaagtat aataaaaagc
      601 cttgtaccaa gatgggaaga tggtttaaag cttaagcttt tttataattc atcaatatat
      661 aaaaataaaa atacagttgg aataaaagag ataagacaaa taggttgtga gtatctagga
      721 gaagcctctg tagaagcaga tagagaagta gtgaaattag ctcttaagat acttgaaaaa
      781 tacaataata attttattct agaggttggg agtagcaagt atattcatgg tttgttagaa
      841 gaattaaatt taaataagaa ttgtgaaaat caaattaaaa atttactata tacgaagaac
      901 actcatgaac taaaagtata tattgaagaa ttaaaaatta agcgtgaagt aaaagagtta
      961 ttatcaaata ttttaagttt acaagggaat ttatataatg ttattgaaaa atcaaacaag
     1021 ttctattgta ataataagat gaagcaagct cttgaagaat taaagcaagt aaataattta
     1081 attgaggaat gtaatttttt agataaggct agatttgatt tatcaatgat tactatgctt
     1141 gactattatg aaggaattat gtttagagga tactacccaa actcatataa agaaattctt
     1201 agtggcggaa gatatgattc tttaactaaa gagtatggaa aagaaattcc agccattgga
     1261 tttacactaa gtgttgatga gttaatgaag tatgtatata agtaaagttt agaaggaggg
     1321 attgctttgg attatttgac tatagcacta gcaaaaggac gaattgaagg ggaatcattt
     1381 aaaaaattta aaaaaatggg tctaggagac agtatagaca cagatacgag aaaactaatt
     1441 tttaaagatg aagaaaataa aattatatat atacatgtaa agccatcaga tgtagttaca
     1501 tatgttgaaa aaggagtcgc tgacctcggt atagcaggta aagatactat tttagaaaat
     1561 gagacagatg tatatgaaat atatgattta ggttttggaa aatgtaaatt tgctgtagca
     1621 ggattaaagg gagatagcat atatagagaa gatgaatatt taaaagtggc aacaaagtat
     1681 cccaatatag caaaaaagta ttttaaagag aaaggtcaaa aaattgaaat aataaaatta
     1741 aatggttctg tagaactagc tccaatagta gggttgtcag atgtaattgt agatatagtt
     1801 gagactggca atacacttaa agcaaatggt cttgaaatat tagaagacat atgtaatata
     1861 agtgctagaa ttatatcaaa tagagctagc tatagattta aatatgaaca aattcagaat
     1921 ataattcgtt tatttgaaga acttgataat taaaatataa ttgagcttta taaggaggga
     1981 tttaagttga gagaaaaaga aagcataaga gagttaaggg gatatgaacc aaatcacgta
     2041 aactgtaaag taaaacttga tgcaaatgaa ggaagtaaga ggttgtttaa ataccttatt
     2101 aaagaaattt cagatagtga tatagattta aatttatatc ctgaagattc ttatagtgat
     2161 ttgaaagaat ctataataga ttacataaat atttctggag taaataagaa aaatcttcta
     2221 gtaggaaacg gttcaagtga aataattgat ttaataatac atacttttgt agataaagat
     2281 gaagtaatat tgagtttctc tccaagtttt agtatgtatt ctatatatag tcagataaat
     2341 gggtctaaat ttataggtgt tgaaagtgat gaaaatttag ttataaatat agataatgtg
     2401 attgaaaaag taaaggagaa taatcctaaa atagtaattg tatgtaatcc taataatcca
     2461 actggaacaa tattaaagag ggaagagata ataaagcttt tagattcaac taatagtcta
     2521 gtggttttag atgaagcata tatggatttt ggagaagaaa gcatgttaag tgatgttttt
     2581 aagtatgata atctaatagt tcttagaact ttatcaaagg catttggatt ggctggaata
     2641 agaacaggat atatgttatc taatagtagt ttaataaatt ctgttgagaa agtaagacct
     2701 ccatataatt taaattcatt atcagacttt attgctacaa gagcacttag aaataaagat
     2761 gtggtaaagg cttatataaa ggaagtaaaa gaggaaagag aagttttata tgaagaaatg
     2821 attggtatgg gaattaaagc atacaaatca caagcaaatt ttatattatt ttattctgaa
     2881 atcgaaaatt tatcacaaaa acttattgat agaggtgttt taataagaaa atttggtgga
     2941 aagttagaaa attattacag agttacaatt ggtgataaag aagaaaattc tatgtttgtt
     3001 ggagctataa gagatatttt gaaaaaggag aaataggtat gagaatttgg aaggtagaaa
     3061 gaaatactct tgaaacacag attttagtag aattaaatat tgatggttct ggtaaagctg
     3121 aaatagatac tggaatagga tttttagacc atatgcttac cttaatgtca tttcatggca
     3181 agttcgattt aaaagtgatt tgtaagggtg atacatatgt agatgaccac catagtgtag
     3241 aagatatagg tatagctata ggtgaagcat ttaaaaatgc actaggtgac aaaaaaggaa
     3301 tacgaagata ttctaatatc tatataccta tggatgaatc tctatcaatg gtagcaatag
     3361 atataagcaa tagaccttat cttgtattta atgctaaatt tgatacccaa atgattggta
     3421 gtatgagtac tcaatgcttt aaagaatttt ttagagcttt tgtaaatgaa tcgagagtaa
     3481 cattacatat caatctctta tatggggaaa atgaccatca taagatagaa agtattttta
     3541 aagcatttgc aagagcttta aaagagggta gtgaaatagt ttctaatgag atagcatctt
     3601 caaagggggt tttgtagaat gaatataata gtagactatg gtcttggaaa tatagattct
     3661 gtgtctaggg gttttagaaa agctggaata gaaactaaaa tttctagtga tatagatgaa
     3721 ataaaacagg cagattcact tatacttcca ggtgttggag cttttagaga ttctataagt
     3781 gccttagata aactaggatt gataccaata ataaaggagc atgtttctaa gggtaaattt
     3841 atgataggta tatgtctagg aatgcaatta ctttatgaaa aaagttatga gtatggagaa
     3901 tatgaaggat taggacttat aaaaggaagt attgataaac tggatataag tttaaaagtg
     3961 ccacatatgg gatggaataa tctaaaattt aataaggcaa atgatgatat acttaaatat
     4021 ataaatgaag atgattatgt atacttcgtt cactcatatt atgcaaattc atcaaatgaa
     4081 gaactaattg cattttcaga atatgaaaag aaaatccctg ctattgttag aaaaggcaat
     4141 gtatatggta tacaatttca tcctgaaaaa agtggagaag tagggttaaa tatattaaga
     4201 gcatatgggg agatgataaa atgataatat ttccagcaat agatataaaa gataataagt
     4261 gtgtaagact cacacaagga gaatttgaca aagtaaatgt atattatgat aatccattag
     4321 aagtagcata taaatggaaa aatgaaggag cagaatatat acatatagta gatttaaatg
     4381 gtgctagaag tgaatttggt gtaaatacaa aaataattga ggacatagca aacaatatag
     4441 atatacctat tcaagttggc ggtggagtta gggataagga aaaagtcaaa agtttaataa
     4501 atgctggtgt tactagagta atattaggta gtatagcgat tgaaaatttg aatttagttg
     4561 aagaattggt caatgaatat aaagagaaaa ttgtagtttc tatagatgct aaagatggaa
     4621 aagtagcagt taggggatgg gaagttgtaa gcaatgtaga ctcattaaca ttatgtaaac
     4681 aacttgaaaa gataggtgta caaactattg tttatacaga tatatcaaaa gatgggatgt
     4741 tgcaaggtcc aaattttgac atatatgaaa gaatagcaaa ggaaacatca ctaaatgtta
     4801 ttgcttctgg aggcgtaact tctatagaag acgtaaaaag actaaaagct atgaacttat
     4861 atggtgcaat aataggtaaa gcgctttatg ataaaaagat agactttaag gaggcacaac
     4921 aattatgctt actaggagaa taataccttg tctagatgtg agaaatggaa gagttgtaaa
     4981 ggggaaaaaa tttaaagata tagtagatgt tgatagtcca gaagtacttg gaaaatttta
     5041 tagtgattgt ggtgcagatg aacttgtatt ctatgatata actgcatcaa atgaagagag
     5101 aaagacatct ttagaatttg tgacaaaagt tgcagaaaat ataaatatac cattttgtgt
     5161 tggtggagga gtaaataaat tagaagattt cactgatatt ttaagaaaag gtgctgataa
     5221 agtttctata aattcatcag cagtcaaaaa tcctgaatta ataagggaag cttcattaaa
     5281 atttggagca caatgtgttg ttttatcgat agatgcaaag aaaaatgaag agggttcatg
     5341 gagcgtatac gtaaaaggtg gaagagaaaa aactaatctg gatgcaattg aatgggctgt
     5401 taaaggtgta gaacttggag caggagaaat cgttgtaaat agtatggatg aagatggaat
     5461 gaaaaatgga tatgatatag aattattatc aaagataact tctttagtaa atgtacctgt
     5521 tatagcatct ggaggcgctg gaaaaaagga agatttttat gaggcggtta ataaatccaa
     5581 tgtagatgga attttagcag catctgtttt tcattttggc gagataaaaa taaacgattt
     5641 aaagaaatat ttaaaagaca tgggtgtaga agtaagatta taagttaaat actaaaagga
     5701 gattagaaat atggataata agtgtaacaa tgtatacagt gatgaagtag agaaatttat
     5761 aagaagtata aagtttgatg ataaagggtt agtccctgtt gttgttcaag aggtagttag
     5821 caaagatgta ctgatgttag catatatgaa taaagatgtg ataaagaaaa ccttaaaaga
     5881 taaagtagct tgctatttta gtagaagtag acaagaactt tgggttaaag gtgagacatc
     5941 tggtaatact caaaaagtag ttaagatgtc ttatgattgt gatgtagata ctatactact
     6001 ttttgttgaa caaactggtg tagcttgtca tacaggcaat tattcttgtt tttacagaga
     6061 cttgtttgat gatagttcaa aaatggagct tgaagtacaa acaaatatat tgaaagaatt
     6121 atatgattta atcaatgaaa gaaaaaataa tccagttgaa ggttcttata ctaattatct
     6181 ttttgaaaaa ggaatagata agattcttaa aaaagtaggt gaagagagta gtgaggtaat
     6241 tatagcatca aaaaatactg ataaaagtga acttatttac gaaattagcg atttagtata
     6301 tcatacattg gttttaatga tagaaaaagg tgttgaaatt gatgaaataa aaaaagaatt
     6361 acttaaaaga agaaaataga ata
//
LOCUS       DAMJYX010000146         6371 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJYX010000146 DAMJYX010000000
VERSION     DAMJYX010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.151"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(231..722)
                     /locus_tag="PC479_003256"
     CDS             complement(231..722)
                     /locus_tag="PC479_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2805775.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
     gene            complement(1816..3951)
                     /gene="rnr"
                     /locus_tag="PC479_003257"
     CDS             complement(1816..3951)
                     /gene="rnr"
                     /locus_tag="PC479_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2805776.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            4544..5158
                     /locus_tag="PC479_003258"
     CDS             4544..5158
                     /locus_tag="PC479_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2805777.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            5176..5538
                     /locus_tag="PC479_003259"
     CDS             5176..5538
                     /locus_tag="PC479_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805778.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            5644..6141
                     /locus_tag="PC479_003260"
     CDS             5644..6141
                     /locus_tag="PC479_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2805779.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         2154 a          936 c          724 g         2557 t
ORIGIN      
        1 cttttaagat aaataaaaat atctacaact atatattgca aaaatttaaa actaatttat
       61 atatgctaat ataaatattt ataaagacat aagacaaatt tacctacaga tttaaattta
      121 acttatatat tttaaaaggc taatataata tgtattttct tctatacaat caaaaggctt
      181 catatctaag atatgtgatg acactaatat tcacaatttt ttagtaatta tcacattata
      241 aaaaatatta cagctagtcc atcatcagat gcttcttgta taaacttttt aagctcaatt
      301 agatttgccc atacatattc aaaaaaatca tcttcactct catcattcat aacaggataa
      361 atattatttt ctaacatctc attaatatta aatctttttc taaatgcttg ttcttccaaa
      421 ctgttcattg cttttgatag tttttttaca tcatttgctg taatcaacat gacaggaaat
      481 tctccatcaa tttccataag tatgtttcca cttagtacta atttgctaaa tatattatca
      541 tcatctccac caaaaggaca acctgtcaaa gtaaagtgaa tggcatgcca tgctttatca
      601 atattgatag tactattttc gttggcatct tcaaaaacaa aatcttgtaa gttttcttct
      661 gcttgctgta atttaaaaac attctcttca cttattttag tataacatcc tatcatcccc
      721 ataattacat tcctcctaag tagcgttttt ttaattgtaa cttataatta aacttagtta
      781 caataaaata tattgagaaa agtattattt catcttaaat ttaaatacct atattatcaa
      841 ctaaaattct gtgtttatag cgtaagtatt attatttttt cctaaaaatt aaatgaatca
      901 gaagttctat aggcaatatt gactataaat aagaactatt acttttaaaa aaaaatttaa
      961 gaaattatac tgtagaatca tgctctactt attagcttta aataaaaaat aaatcatgtt
     1021 ctcaaattaa ataataaata acaataaatt taaattttaa actataagag tttctgaagt
     1081 gttaataagc ataccaataa ttatatgtgt aagaatttga tttaatagat atgaataatt
     1141 tttacattaa ttataaatat attaacctat tgttataaat tctaaaaaag aaaagttatc
     1201 ttaaataaaa aataagtgta tcttaaattt taaatgatac acttattttt atattgaaat
     1261 tatatttttt aatatctatt cttaattagg tatttttaaa ttatctgttt acattaaata
     1321 tttcttaatt acttatcttt cagctagctt tttctattaa atgtttctca attacttatt
     1381 tttcaattag ccacttatat taagcatttc ttaattactt atttttcaat tagccactta
     1441 tattaaacat ttcttaatta tttatttttc agttggcttt ttctattaaa tgtttcttaa
     1501 ttacttatct ttcagttagc tctttctatt aaacatttct taattattta tttttcagtt
     1561 ggctttttct attaaatgtt tcttaattac ttatctttca gttagctctt tctattaaac
     1621 atttctcaat tacttatttt tcaattagct actttcatta aatatttctt aattatttat
     1681 ctttcagtta actatttcca ttagatattt ctcaattacc tatttttatc atttatttta
     1741 tagagtattg tgcactatat atttatacat atcataaata ctacaacttg ttatctctta
     1801 ttttagctgt tttatctact cctctatttt ttcaactatc tcaaagtcaa tttctttaaa
     1861 atctacatta acactttcaa cctttatctt aacaaggtct cctatcttat atgacttctt
     1921 agttctttct cctaaaacac tataagaact ctcatcataa atatagtaat catcattcat
     1981 atttgcaagt cttatcaatc cttctatggt attatccaac tcaacaaata ttccaaatga
     2041 agttacgcta gatacaacac cctcaaattc ttgaccaact ttatccttca tataaagagc
     2101 cttatagaaa tcatgtacat ctctttcagc taactctgcc tttctttctt tctcggatga
     2161 ttgaacagat gcataatcca ctatagtagt aagctgttct tgtctttttc cacttatttt
     2221 attatttaaa gattctttta ttattctatg aatttgaagg tcaggatatc ttcttattgg
     2281 tgaagtaaag tgacagtaga atttagctgc taatccaaaa tgtccactac attctggtga
     2341 gtacttagct tgttttaaag aacgaagcat tatagtactg atagctcctt cttctttttt
     2401 gccctttatt ttctctatta taccttgtaa tgcttttgga tgtacttcct ctttatcacc
     2461 ttttattata tatccaaatg tagatataaa cttactcaat tcttccattt tttccaaaga
     2521 tggtgtctca tgtattctat acacaaacgg tattcccatc cagtaaaaat gttcggcaat
     2581 tgtctcatta cttataagca taaattcttc tattatctta ttagatattc ttctttcata
     2641 tggctttata tctacaactt cgccttttcc atttagaatt atctttgcct ctgggaaatt
     2701 gaagtctata gctcctcttt tttcccttct tgacataaga atcctagcta aaatttcagc
     2761 atttttaaaa tcttcaacaa catgttcaaa agtcttttta agtttttcat catccttttc
     2821 taatatatca gatacttctg tataagtcat tctagctttt gaatttatta cagattcaca
     2881 tatttcatgt ttaaccacat ttcctttatg gtctatttcc ataaatatag aaagagttaa
     2941 tttatcttca aatggattta agctacatac tccatttgaa agagtttttg gcaacattgg
     3001 aattacttta tctactaaat aaactgatgt agctcttttt aaggcttcct tgtcaagttt
     3061 atttttctct cttacataat gtgtaacatc agctatgtga actcctagtt tgaagtttcc
     3121 attaggtaat acttctattg atatggcatc atctaagtct tttgcgtcat ctccatctat
     3181 agtaaatatg tttaaatctc ttaaatctct tcttcttttt atctcttctt gaggaatctc
     3241 aacagctaca gcttctgctt cttgcaatac tttctttgga aattcttctg gaagaccatg
     3301 ttctcttatt atagaatcta tttctacgcc tctttcacct ttttgaccta gtatctctat
     3361 aattttgcct tctggttttc tatcctcttg tggccatact gtaatttcac atactacctt
     3421 atcattatct actgctccac taaaatgttt tttaggtata tatatatctt tagtaaattt
     3481 cttgttgtct ggtgttacaa atccaaaagt tttacttgat tggaaagtac ctactattct
     3541 agttatttct ctctttatta cttttactat tcttccttct gctctctttt cttcagttgc
     3601 aggtactact atttctgcca taactctatc attatgcata gcaccattta tattgtcact
     3661 tgagataaat aaatcctgtg tatattcaac atcagattct acaaatccaa aacccttttt
     3721 gtgtgataca aattttccaa caaagtatcc catttgattg ggagatacta tttttccttt
     3781 tttagtaaag cagatataac catcttcttc taattcattt aaaaaattat aaaacattgg
     3841 catttctgat gaatgtatat caaatatttc tgctaattct tcttttttta gaggattata
     3901 tgcactttca tttattaatc ctagcaaact ttctttaagt cctggtatca tttttccctc
     3961 ctgttttact tttcattata ttattattta tattgtcttt tatatttctt cagacaaaac
     4021 attttttaat tatcacataa ttttctatat tttcttatta ttataagtta aaattactta
     4081 tttatatttt tgatttaatg taagctttgt catactaact tcaaacaaat tatgatgttt
     4141 tataaattat ttgtaaatat cattataatt acttaagtta ttctttatca taattgctta
     4201 aattactctt tatcacctaa ttatacacca tcaaatagga ttggtaaaat attcatctca
     4261 tataagtatt actatataat aatttattct attagtatac tcaaatcatg taaaatttat
     4321 ataactcaat taatttttta aatacaactt tataatataa aacttataat aaaagtaaat
     4381 ttatatgtta aatgaggttg attttttatt attttttatt tatgctagta ctaaagactt
     4441 gcaaacgttt tttgacaagt ctttattttg tctaaattaa acaaactttt ttacaaataa
     4501 atacataaaa tttaaactaa tctatttatt ggaggagata aaattggaag aagtaattgc
     4561 aaaaattatt actatagaaa atggattcaa agaaattgaa aacattgcta aaaaaattgt
     4621 aaaaaataat gactcaaaaa agtgttatta tctatcaatc tacttatata ggtcagaata
     4681 ttatcaagta agagaattgg ctgtgtttat acttggtttt atatcagtaa ctatttctga
     4741 agctttgtta tttttaaaag ataatgtgag taaagatgaa aactggagag tacaagaaat
     4801 actagctaaa tcatttgatt attattgcag tgaagtagga tatgaaaagg ctcttcctgt
     4861 tataaaagaa tggcttaatt ctgatagtcc aaacataaaa agggctgtga cagaaggtct
     4921 tcgaatttgg actagtagag actattttaa atctaatcct gacgttgcta tatcactact
     4981 ttctagttta aaagaagatg atagtgaata cttgagaaag tctgtaggta atgctcttag
     5041 agatataagt aaaaaatatc cagatttagt aaaaaaagaa ttagattcat gggatatatc
     5101 aaataaaaaa gttgagtttg tatataagct tgcttcaaaa tgtatcttgg caaagtaaaa
     5161 tggaggtgaa cttatttgaa acttcgtgat gagtatactt gcccattaga actaacacat
     5221 gatatgataa aaggcaaatg gaaatctatt atattatgga gattacgcat aggaccaact
     5281 tccttatcaa aacttgaaaa ggaaatcaat ggtattaatc aaaagatgtt acttgaacaa
     5341 cttaaagaat taattgaatt tggtcttgta gataaaaaga catatgaagg ttacccactt
     5401 aaagttgaat actcacttac acctattcga gggaaacaaa tattggatgc tttggctata
     5461 atgcaagata taggaattag ctttatgttg gataatggta tggaagatat tcttaaggaa
     5521 aaaggtgttt taaaataaat agatacccac aaaaaagtaa gtaatttacc attattttca
     5581 actggtatat aattaaatta tagatatatt ctattaattt attataatag gaggtaaatt
     5641 attatgaaag taactagtac aggtatagta aatggtgtta tagaagataa atacggtaaa
     5701 agaggaactc agttcaatga aggtggtatg cctacatatt cactacctct aaagtttgaa
     5761 gatgctccaa agaatacagt atcctttgct gtatttttag aagataaaga ctctgttcct
     5821 gtatgtgggt ttgcatggat acattggtta gctgtaaata taacaaaaga tgagttaaat
     5881 gaaaatgaaa gtgtaacagc tactgatttt gtacaaggga ctactagttg gcatggcaaa
     5941 ctaggaggtc ttgacagatt agaggccagt gtatatggtg gaatggctcc tccagacaga
     6001 cctcatgtct atgaaataca tgtatttgca cttgatacta tccttgacct tgaaaaagga
     6061 ttctatatga atgaattttt caaagctatt gaagggcatg ttttagatac atttacacta
     6121 aaaggaagtt attctaatta attaggttga ctatagctaa tcattaatta aatgctacat
     6181 ttaattgatg attagcttaa ttgttattag aatattttct gcactttatc ttaatatata
     6241 ttttagttga ctggtatttt aatttctatt agattattat caatttatat aaattgattt
     6301 tagcttaaaa cttacttatc tatttcagtt tctattagaa ttattattag actcattaaa
     6361 ataaaagcac t
//
LOCUS       DAMJYX010000147         6364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJYX010000147 DAMJYX010000000
VERSION     DAMJYX010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.152"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            242..952
                     /locus_tag="PC479_003261"
     CDS             242..952
                     /locus_tag="PC479_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805780.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
     gene            1475..2812
                     /locus_tag="PC479_003262"
     CDS             1475..2812
                     /locus_tag="PC479_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2805781.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(3148..3717)
                     /locus_tag="PC479_003263"
     CDS             complement(3148..3717)
                     /locus_tag="PC479_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2805782.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            4087..4692
                     /locus_tag="PC479_003264"
     CDS             4087..4692
                     /locus_tag="PC479_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HDF2805783.1"
                     /translation="MTNWNLWRTLNMEYIINPCSGIKIKITRGQKIEIVDIEGGQVVD
                     FFAEMKDDSTEFISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPC
                     CRPEMYDFFYHNGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLS
                     KANDKIILEALENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(5371..6273)
                     /locus_tag="PC479_003265"
     CDS             complement(5371..6273)
                     /locus_tag="PC479_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2805784.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         2331 a          977 c          753 g         2303 t
ORIGIN      
        1 ctttttataa taataaaagc atttgtattt tataataaga aagattgtta gattttttaa
       61 taagaaaatc attagcactt cagaataagt ttaatagaat agttttaagt gtaagtatac
      121 taatataaaa aattttattt tgctatctgg atataattgt tatttcattt tatatatcta
      181 ggtagcaaaa tatattatat acttaactat ataaatataa aacttgaaag gagataaaaa
      241 attgacttcg atttgtattt gtgatgatga aaaacaacaa agaaacatgc tgaataaaat
      301 cattacaaga gaaatggatt tgtggggcta cccctaccaa ataaaagaat attcaaatgg
      361 agaaagtcta gtatacaatc tagaacacga aaattattac atagatatta tttttttaga
      421 tattgaactt ggaaaaagta atggcgttga catagcaaaa actatacgaa aacattcaaa
      481 agatactata atcatctttg tcacaggttt ttctgactat gtatttcatg gatatgatgt
      541 aggagctttg aactacatct taaaacctta taaaactgaa aaaatatgta aagtattaaa
      601 agaagcacta aaaaaattag aacaatggaa agaaaacttt attacagttc agattggaac
      661 taatctttgt aaaattaata caaaagatat cctctacttt aaaagtgagc taagaaaatt
      721 aactgtagtt acctttgata aaacattaga atactacgga aaattaaatg atatggaaac
      781 tgttcttccc tctaatttta tacgtacgca tcaaaggtat attgtcaatc taaaccatgt
      841 agatagtata actcaaactt atgccaaagt tcagaatgaa aaaattccta taagccgtaa
      901 aaaatatcaa gaggttgtag gaaaatttac aaggtatatg ttagaatctt aatttttgaa
      961 gttcaatctc tagaatttta tttttgaaac ttaacttttt aaagcttagt ttttagagtt
     1021 ttgttttttc aaatttaatt ttttgaagct taatttctag aatttcaatc tttcaaaatt
     1081 taattttttg aagcttagtt tttagagttt ttttcaaact taatttttta aagctcagtt
     1141 tctagaattt caatctttca aaatttaatt ttttgaagct tagtttttag agttttttca
     1201 aacttaattt tttaaagctc agtttctaga atttcaatct ttcaaaattc aattttttga
     1261 agcttagttt ttagagtttt tttcaaattt aattttttga aacttaattt ttaaaatttc
     1321 aatcttttaa aactcaattt tttgaagctt aatttctaga attttatttt tcaaaatcaa
     1381 ttctttgaaa tctaatttcc agaattttaa tttttaagat ttaatttttt aaaacttagt
     1441 ttccagaatc ttaattaata gaaaggtata ttaaatgggc acttattctt taatctctac
     1501 aagcataaat tttattctaa tattaatcaa tattttcata gccttttttc tttataagtt
     1561 gacttcctat tttatagact ttaggaatca ttggattttc aaaatcatat tgattttggg
     1621 ctactctatt attagtcaaa tgataatatg gctttccgac cccataaata ttttgtttac
     1681 actattcgga tattttatac tacttataat atgtagcaaa agcaaattaa ttcctataat
     1741 atcagttgtc atgatacttt atcctattat aattgggctt aattttcttt ttataaacaa
     1801 ccctatatat gaggaactta tacactcaaa agagtatatt ttaatacttt caatagtcca
     1861 gataatttct gctctaatta aattattatt ttggttttta atacatattt tcctaaagaa
     1921 tagattggtc aatataaaac aatacttatc aaataaaata tggattttta ttgatatggt
     1981 atgtatatct tcatttttat caatattaat tgctattatt ctgccaccta ctaatttaat
     2041 ccctatgggt agtaataaga atattatctt aggcaactta ccttatggca cttatctaat
     2101 catcttgtca gcaattctgg caaatatcgg tgtgatactt cttttacagc cattgattga
     2161 aaatgtaaag atgaagatag aaaaacaagc aaatcaccta aaagaaaaat actataactt
     2221 actagaaaat caacaaattc aaatacgcaa aatgcgacat gatatgaata atcactttca
     2281 aatgattgaa tcgtatttag aaattgaaga tattgctgga gctaaaaagt attttaatca
     2341 gttaaaaata ggtataggta ctcttagtgg aaaacaattt tgcaaaaacc aagctcttaa
     2401 ctcaatttta aatcttcgtc atacaagttt agaagaaaat ggtgttgatg tgcactttaa
     2461 tatagatata gacaatcact tgggtataga acctatagat ttatgtacta ttttttcaaa
     2521 ttctttagat aacgctatag aagcatcttt gaaaattcct gatttgtcaa atagaaaagt
     2581 ggtattaaag gcacgttgtg agaaggagta ttttagctat ttattgacaa acaacaaggt
     2641 aaatcctatc aatagaaaac aagaactatt tatttcagat aaaaaagata gtaattctca
     2701 cggatatggt attgagaata ttagagaaat tgttaataag tacaatggaa aattggatat
     2761 ttcttatact gagagtgaat tttctttatt tctttatatt agattatttt agtctaaaat
     2821 tagattatct tatataatac aaattagatt gtttgatact atgtaaaatt ataataactt
     2881 ataattaaat atttacacat atattcatta acttaaaatt taatcaaatg tgatatttta
     2941 tttaccttag taaaaatgtg tgtgtctcaa aataaacttt ttagttgatg aggcactctt
     3001 ttttacatga aatcaaatat atttttatcc tttcaacaat gaaaaatgga tttatctctc
     3061 cattttgaaa caagcccttt ttctattaat tactttctta aaaaatataa tacgatatat
     3121 aagataaata tctaattgta cctatttcta attatatcta ttagaataca acacctttgc
     3181 cttttcagta aactccctaa caaaatctag tttactatct gtataagcat cacgattatg
     3241 ctcatacttc ttccaaaggt ccaacttaag tttttcatac tcctttgcaa catctgggtg
     3301 gtcaattaag taatctctaa aaaacaactc atcattatca cctacatacc ttatatgcaa
     3361 atgaaaaact cgttcttcaa acccctctac tgtataccct ttattaagtg aaagcctact
     3421 actgctctca gacatacaaa tatatccatt cgactcaatc aaatctttaa tatctttcat
     3481 attataatca ggaattatct caatcagtat gtcaacaatg ggcttggcat aaatcgtact
     3541 cacagaagta cttccaatat gacttattct aacaattttt tgcattggca atattccagt
     3601 tagtctatat tgttcttctt taaaccaatt tttccaacat tctttgtgag tagtcaaaaa
     3661 tataggaaac aagttccata actcctctaa agtcatatct tccaacttct ttttcattag
     3721 ttacctcctt ttaaatttgt actttatatt ttttcttttt caaatttact tagttaatat
     3781 ttaactttat tttttatcaa atcatttttc taattatgta aatttttcta aatttagttt
     3841 ttaatgtatt ataacagatt tttacccttc ttacactttt gtatctattt aaatattaga
     3901 acattttttc ttacttctaa atacagtaag attgcttaaa taccaaatag gagcaaaaaa
     3961 actctgtaat ctaaaaaaaa ctacaacatc tttatgagaa ttaaaaaatg atagactatg
     4021 ttatcaacat taagaaacag atagaaaaga aacagagtat gtgctaaaat gggaacatag
     4081 aacagaatga caaattggaa tttatggagg acattaaata tggagtatat aatcaatcct
     4141 tgtagtggta taaaaattaa aataactaga gggcagaaaa ttgaaattgt ggatattgaa
     4201 ggaggacagg tcgttgattt ttttgcagaa atgaaagatg attcaactga atttatatct
     4261 ccaggtgtta caattgattg taatgagtca ttaagattac aagttgggaa tttaatatat
     4321 tctaataagt atagacctat gtttaaaatt ttatatgatg atgtaaggga acatgacttg
     4381 ctacatccat gttgtagacc tgagatgtac gatttctttt atcacaatgg agaaaatcat
     4441 ccaaactgtt ttgataatat taatcgcaca cttggaactt cccatcaaat tattcacccc
     4501 attaatttgt ttatgtacac aaaagtcaat acagatggga gtattgaagt atgtgaacct
     4561 ttatcaaaag ccaatgacaa aattattctt gaagcattgg aaaacatgac tttagggatt
     4621 gctgcatgca gtgtttcaga aagttcttgt aatagcagaa aatgtacttc tattaagatt
     4681 ttggttgatt gacaaatttc atttatcgaa aaaatatcaa aattaaaaaa tgacgtcaaa
     4741 ggcgcaaaag ggtaaccact tgcgctctca attcattatg gtttgttacg tatttatggg
     4801 taaaaatccc ttgattctat gatgtttttc agacacaatt acaaatgagt aaggatttta
     4861 tactcatact ctaaaaaata gcattttatt gcgtaacaga acgaaaaaac agtgtgtacc
     4921 tttttcttgc aatacacact gtattcatag ccactctagc tgttaactgt taattatctc
     4981 ataataccct tactggtatt catctaatct taaccccttt taattatttt catttataaa
     5041 taaattctat tcttcccaaa atatcgtaat taaaatatat taattaaaat atcccaataa
     5101 gaatatctga aaaatcttct acctattttt tcaaaccttt tattcccatt cctcatacaa
     5161 tagtgcaact tttagcacag tataaatata ccattagtcc atataatctc attaacaaat
     5221 atatacaatt attttcttca aactctttta tatctaagat atcacatttt cattttctta
     5281 tattaattcc ttaactctct ttcaaattac caatttttta atcaaatctt cattaaatat
     5341 tctatcatta ttaaacattc atcattatcc ctaaaaaata tgtgccgtat gttctttaca
     5401 caaattttcc atcttatcat acacagcacc actatctctc tcatctgtca tagtcacaat
     5461 tacatcactt ggtctaagaa ttgtcttacc tctaggaata atttcttctc cacctctttg
     5521 aattgctacc aaaagacaat tttcaggcca ctgtacatcc tgaattgcct tgttcgcaac
     5581 aggagagcca aacataacag agaactggtc tagtatcttt tcgcccctct ctttatgtac
     5641 aggttctcca ttcttttcaa gtaacctctc aagaagactt tcataaatag gtttagactt
     5701 cataagtgta gccacaatat atgccacaat tgaaattact gatagtgaaa gcatttgact
     5761 aacagaacct gtcatttcaa aaattaatat gataccagtc aaaggggcac gcacaatagc
     5821 tgtaaaatat cctgccatag ccaaaagcac aaagttattt acataatcgg gattcattcc
     5881 aaaaacttta acacctacca taccaaatat tccacctata aatgctccta aaacaagcaa
     5941 tggaaaaaat attccccctg gtgcacctga cccaaaacta acagccgaaa atataaaacg
     6001 tataacaaaa atcaaaacaa ccattccaaa aatatattta tgacttgtaa gttcttcaac
     6061 taaattgtgt ccactcccca gaacatcagg cataacaagc ccaagaatac cagccattat
     6121 aaatggaatc aacagttttc caaacgtacc aattttagga atcttcttgt ataaagactg
     6181 cacatataga gtaaaccagt tgtaaaatgc ccccataact ccaagaataa cacctaaaat
     6241 cactattaat ccataataat cttgtggcaa cacatttcca atctgaacct cccatgacta
     6301 aagtcacagg gttcctgctt catagaattt tgcatactaa aatatgtctt actaattctc
     6361 caca
//
LOCUS       DAMJYX010000148         6324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJYX010000148 DAMJYX010000000
VERSION     DAMJYX010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.153"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            127..3306
                     /locus_tag="PC479_003266"
     CDS             127..3306
                     /locus_tag="PC479_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2805785.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3487..3570)
                     /locus_tag="PC479_003267"
                     /pseudo
     CDS             complement(3487..3570)
                     /locus_tag="PC479_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3591..4355)
                     /locus_tag="PC479_003268"
     CDS             complement(3591..4355)
                     /locus_tag="PC479_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2805786.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4511..4744)
                     /locus_tag="PC479_003269"
     CDS             complement(4511..4744)
                     /locus_tag="PC479_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805787.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4818..6311)
                     /locus_tag="PC479_003270"
     CDS             complement(4818..6311)
                     /locus_tag="PC479_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2805788.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2345 a          904 c          739 g         2336 t
ORIGIN      
        1 tgttatttac acatatatcg tgatagataa aagctattca tatctgcctt aattttagtc
       61 ttaaatttag agtgtaaatt gaatgcattt agattataat atatacagtg aggtaaggtg
      121 aataatatga aaatagatat gatatataaa ctaataagaa gaaatacaga accatcaaga
      181 tggtcaagag gtgtaaaata ttatactaat caattagttg attatataga catagatatt
      241 gatgacctac aaaataatct gttgacattt cgtggtggag tcaattctga agataattat
      301 agcttttatt ttaattcaat aactatagat ataaagcact ttaagataat tgaaaatgat
      361 tgtagttgcg ctgactgtca atcaaagggc aaaagtattt ttatatgtaa gcatatagtt
      421 gctattgtga taaaaagctt aaaacaaata aaaaataaaa aagatgttcc tcctattcta
      481 atagatatat taggtgcaga taaggataag ctttctaata caaattctaa aaccagctta
      541 gatttaagca agcaattact ttctttgtta gacaatacta aaagagaaag ggttaattta
      601 gatgtattga taaatgtgga ttcaaataaa gcaattgctg aatttaaaat aggaattaat
      661 aaaatgtaca ttttaaaaaa cattactgaa tttgcatatt caaaacttaa tggatatgaa
      721 ttagattttg ggaagaaatt tacttacaac cccaacactc attattttgc tgatgaagat
      781 gagaagttag tagatattat agaagaatat gggacaaatc tttcatacaa cttttcttca
      841 tcacattcaa aaaaatttat ggagataaat gcaagctctt taaaacaatt tttaaatgct
      901 attaagtaca aggattttac ccttattttt tctaaagagg aatatcatcc aaagatatta
      961 aatgatactc tacctgtaag gtttgatttt aaaaatatag aagataaaat agtcttatat
     1021 tctaaagatt cactacctgt tccacttact aaaaaaaatg atgtactttt atatgatggc
     1081 aatatctatt tattatcaca taaaaaagct catgattatt caaaaatata tcaaatatta
     1141 tcaaagacta aagagttaaa atttgataaa gaggattcta aggatgtttt aggattgcta
     1201 gttcctagat taaaatctat atctcaagaa gttcatttag atgataacat aaaaaataat
     1261 ataacaaaag attttaaatc tgagttctac tttgatatga ttgatggcaa catatgttgt
     1321 gatgtaaagt atatatatga tgatgaagat aaaaaatttg tgctccccaa catacaaaag
     1381 gaagctacta ttgaaaataa acttacttct tcacaattta caaaggaaaa taatcattat
     1441 gtgtttaaag gaactgacca tgacctattt acatttttag atttacaatt agaatctcta
     1501 aaggaatttg gagatatata ttattctgag aagtttaaat tgaaaaaaat atataacgct
     1561 tcatcaattc aagcttcaat caatcagact aaccagaatt acctagaatt taattttaat
     1621 atatctgata taaatccaaa agagtacaag gatattttga aagcttttca agaaaaacgt
     1681 actttttata aacttaaaga tggaaacttt atagatttat cagaaagaga aacaaaagat
     1741 ttttttgaat tagttgaaaa tctagatata atggataaat caaaaaataa taaaattcat
     1801 aaaaataaag ctctatatat aaatgatgta atacgtagta aaaatttaaa gtttatagat
     1861 ggtaaaaaag aactagacag tatatgtgaa aaatttagaa attttgatag cataaattta
     1921 gaaattcccg caaatttaaa tgcaaacctt cgtgactatc aaataaatgg cctcaattgg
     1981 tttaaagttt tagattatta taaatttggt ggtatattag ctgatgaaat gggtcttggt
     2041 aaaacaatac agactattgc atttttatta tcattatcta ataaaaaaag tcttattgtt
     2101 acaccgacat cactcattta taattggaaa aatgaatttg aaaagtttgc tccagatata
     2161 aaagttttat tgatacatgg aaataaaaga gatagagaaa aatgttttat ggaattagaa
     2221 aattttgatg taatacttac aacatatggt actcttagaa atgatttaga caaatatagt
     2281 gaaataaagt ttgattattg tatattagac gaagctcaaa atataaaaaa tccagtggct
     2341 ttagtaactg agtctgtaaa aagtataaat gcagaaaata aatttgcact cactggtaca
     2401 cctatggaaa ataatctact agaattatgg tctatatttg attttataat gcctggctat
     2461 ctatatagta aagcaaagtt tcaggaattg tttataaata aagaagataa tgttaaaaac
     2521 ctgaaaaaat tgataaaacc ttttattctt agaagaagta aaaaacaagt tatgaaagaa
     2581 cttccagata agattgaaaa aaactttttt gttgaattga ataaagagca gaaaaagata
     2641 tatagtgtat attcaaaaga tatacaagat aaaatgaaag ataaaaatct aaagaaagat
     2701 aaaatagtta tattctctta tttaactaaa ctaagacaac tttgtttaga cccaagtata
     2761 gtagtaaaag attacaataa aaaaagttca aaaattgaaa catgtttaga aatacttagg
     2821 gatagtataa acgaaaacca caaaatatta ttgttttcac aatttacttc tgtgcttaag
     2881 aacataagta aggaacttga taagtataaa attaaatacc attatataga tggtaaaact
     2941 aatgccaaag aaagacttga acttgtagat gagttcaata atagtatgga taaaaaagtt
     3001 tttttaatat ctttaaaagc aggcggaact ggtttaaatc ttacatctgc tgacatggta
     3061 attcactttg acccatggtg gaatccatca gtagaaaatc aagctagtga tagagctcat
     3121 agatttggtc aaaaaaattc agtacaagtt ataaaattga ttgctaaagg gactatagaa
     3181 gaaaaaataa taaaattaca agaaagtaaa aaagagctta taaatcaatt tataaatgga
     3241 gaactttcta atgaaggtgt attaaaaagt ctatctgatg aggagataat aaacttattt
     3301 aactaaatta tttaatccta cagcttaact atatgaattt gttctaacat ttatatagtt
     3361 aagctaaaat atctgaataa ataaaattta atattgttat ttaatcccaa ctttacaatc
     3421 taatgatatc tctttctata catattgcat atacaaatgt aataacttga ctattagaat
     3481 attttgtttt aacaatccta tttcatcata ataatgaagt atatgtacac tcacttttgt
     3541 aagttttgca atccatttta cagttttcat tattgtaaat tttggttaaa ttattcctct
     3601 aaaaatttct ttgctacatt tgaaaaatct aatccattcg ataaaattgc aggatgacca
     3661 cctttatcaa acaaatacat tttaccattt ttaatctttt caagaagtaa tgaaaaagta
     3721 ttttcaaaaa aatcactaga tattagagat acaaactcat cttctctact tcctgttaaa
     3781 agcacctctg gttgcatagt ttctagaggc ttatgaaaaa actttcctat agttttataa
     3841 tgttcaaaaa tagcttctgt atcattatct acaacttttt cccagttttc tccttgatta
     3901 taaatataaa aagcttttgc accatcatct tgcttagatg cttcacgctc tattttcaca
     3961 ttttgtacaa atgactctag aggaacttct ccttcaaaac tatctgcaat taccttattc
     4021 actaaatcag gtctctcaag tgcaacattt aatgcaacaa gagctcctcc actactacca
     4081 attatgttta cttttttata gttcattgct tctaaaaaac atatcacttg catagcctca
     4141 tcaaaccata agtcagcaga aaatttatcg accatttgag attttccatg acctacaaaa
     4201 tcaattaata ttactttata ttcatcttta taaaaattga ttatatcatt aaacatcttg
     4261 gaagaggctg tatttccatg taaaaaaagt aatggtgtac catccccaat ttcattatag
     4321 tagcactgtt tgtcgttata gttaaaatat gccatatatt tccccccttt aatatcccta
     4381 taggtatata tttattactt atatttaaaa tgactattcc cttagccact taaaaatagt
     4441 ataacttaga atgtagataa aatcaacatt ccttgctaat ttatctattg tttgtctaat
     4501 ttattataat ttaacttctg taaaatttct tttttgtacg aaaatattcc ttccatttac
     4561 aacaatcatt caaatgaagt ataacatgac gagtaggagg cataagcaaa attccagcaa
     4621 tatctttatt cccccaaaag tccaacaacc aaatagaatc ttcatgcttt attactcctc
     4681 cagtcaccaa aatcctttct aagtcaattg gagagacctt tcgtttcata tcttcagctt
     4741 tcaaatcctt aacaacttct attgtgtttt ttgcaacttc atttcgataa caaagcaatt
     4801 catcaatatt gactaaatta tcgtatatct cttttcactt taaccatttt tttctttata
     4861 ggtatctcct tatatttttt taatatcaaa tctcccttat aatttaatat atctacatat
     4921 gaacataaat cttgaacttc aattacacct atatcatcat tagttatacc ttttatatta
     4981 ctaaatgcac caactatatc taaaactctt attttcttct tcttaccagc atttaaatat
     5041 aatttaacaa cttcactttc tatatttagt tttatatctt tacttttgta agaattttta
     5101 tcaacttttt ttctctttga tacttttttt aggattcctt ttgaatattc ttcaaacaaa
     5161 actttgccat gtgtaattct acttttttct atatctttta attcatttat tttatatcct
     5221 atatacgttt ctatattctc tatatatttt tcatcctttt cagttactat agtaattgcc
     5281 tttccactat ttccttttct cccagttcta cctattctat gtatatagtt ttctttatct
     5341 tgtggcacat cataatttat tactaaagaa atatcatcaa tatgtattcc tctggacgct
     5401 acatcactac taactaatat attaaatttt tggtttttaa aatccttaat tacaaaaatt
     5461 cttctttctt gtgataaatc tgcatgtaac tctcttatta aaaaaccctc tttactcatc
     5521 ttgttatata atttagatac tttctctttg gtattacaaa atattatcac actctttgga
     5581 ttttcgctgt atattaattc ttttaaaagg atgtacttct ctcttccatt tgccttgatt
     5641 attttgtcat ctatttgtct tgttttttga ttagtatcgc tctcattttc ttctatattt
     5701 ataacactgt aatctagcat atatttttca catatatatt ttatctcttc atctattgta
     5761 gctgaaaaaa gacttactat tttctcctta ggtaaattta ataatatttt ttccatttgt
     5821 tcaacaaaac ctttattaaa cattttatct gcctcatcta ttactaaata ctttacattt
     5881 tccaacttaa tagaacctct atttatatgg tcaagtattc tgcctggagt agctacaaca
     5941 atatgaaccc tttgtttcaa ttcagctatt tggtctttta tagattgttt gccaaatata
     6001 gcactacatc tcaccttctt tagtctccct atatctgata tctcatcttt tacttgcaag
     6061 gctaattctc ttgttggaac cactataaga gcttgaatat tattgtaatc tacatttata
     6121 ttctcgcata aaggtattgc aaaacttgct gtctttccac ttcctgtttt tgacctaact
     6181 actaagtttt gaccttttaa taattttgga ataacttctc tttgtactct agaaggtata
     6241 ttatatccta aactcttcaa tgacttaagt atcttttcat ttaacttaaa tttttcaaat
     6301 gtatatttca tatattacca cctt
//
LOCUS       DAMJYX010000149         6190 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJYX010000149 DAMJYX010000000
VERSION     DAMJYX010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.154"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            102..647
                     /locus_tag="PC479_003271"
     CDS             102..647
                     /locus_tag="PC479_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2805789.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            660..1673
                     /locus_tag="PC479_003272"
     CDS             660..1673
                     /locus_tag="PC479_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2805790.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1895..3868
                     /locus_tag="PC479_003273"
     CDS             1895..3868
                     /locus_tag="PC479_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2805791.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3937..4296
                     /locus_tag="PC479_003274"
     CDS             3937..4296
                     /locus_tag="PC479_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805792.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4331..4687
                     /locus_tag="PC479_003275"
     CDS             4331..4687
                     /locus_tag="PC479_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2805793.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4724..6016
                     /locus_tag="PC479_003276"
     CDS             4724..6016
                     /locus_tag="PC479_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2805794.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2439 a          582 c         1182 g         1987 t
ORIGIN      
        1 acaatattag catatagtac agaaaaaaga tgtaatttgt aaaatttatg gtaagcagta
       61 aaattaaaaa tttaaagata ttatagggag gttataattt tatggaaaac gttattacag
      121 gtctaagtaa aggtgcagag tggtttatcg gtttgtttca gaaaggggga gaacaattta
      181 taggtcttgt ttcaggtact cttccaacgc taattgtatt aatggtagct attaactctt
      241 tgattaagat aattggtgag gaaagagtta ataattgggc ttctaagcta ggaaagaact
      301 cttttacaag atatattcta cttccattag tatcagtatt tttcttggga aatcctatgt
      361 gctatacatt tggtagattt ttaaaagaag agcacaaagc aggattttat gatgcagcag
      421 tttcatttgt acatccaatt acaggtcttt ttccacatgc aaatgcagga gaactttttg
      481 tatggttggg aatatctgca ggtttgacta ctttaggtaa agaaactact acattggcat
      541 tgtggtattt tatagttggt ctaattgtaa tttttataag aggtatagta acagaaaaaa
      601 tgtatgcttt tctaacaaga aaaaatagag caacaaataa ggcttaggag gtagataaaa
      661 tggaggagaa taaaatatta aaaatagaaa agggaacttc aggctgggga ggtcctttgt
      721 atataaaaaa agaagggaat agaaataaga tactatcaat gactgctggc ggtatccatg
      781 aagtaacatt aaaaataaaa gaactactgg gttgtgaaat tgtagatgga tttaaaactg
      841 gagtttctga tgaagaagta gcagtagtta ttattgattg tggtggaact gctagatgtg
      901 gagtttatcc aaagaaaaaa atacctacaa taaatgtaaa tccagttgga aaaacaggtc
      961 ctttagcaaa atttataaca gaagaatatt atgtatcaga tgttaatcct aactgtataa
     1021 gtgttgtaga tggggaagat atgccacaaa aatctcaaga aaataaaagc gaaaataaga
     1081 gttctattag aaagcctgat aattatgatg aggttaagtc aaaagcacaa ggtgaatatg
     1141 ctaaaaagaa cataatactt tctattggtc aaggggcagg gcaagtagta tcaaaatttt
     1201 atgatgcagg tcgagatact atccaaatgg taatgaataa cgtaatacca tttatggcat
     1261 ttgtaagtat gcttatggga attattttag catctggact gggagactgg attgcaagag
     1321 ttatatctcc acttgcaggt aatattggtg gattattgat tatatcagta atttgcacac
     1381 ttccattctt atcaccaata ctaggacctg gtgcagttat agctcaagta gtagggactc
     1441 ttgtaggtac acaaattggt cttggtgcaa taccagcata tttagcactt ccagctttat
     1501 ttgcaataaa tgggcaagct ggatgtgatt ttgtaccagt aggattaagt ttgggagaag
     1561 cagaaccaga aactgtagag tatggtgttc cagctttatt ttactctcgt ttaataactg
     1621 gtccaatagc tgttattatt gcatatggag ttgcagtatt tgcattgaga taatatttaa
     1681 tgtcaatata aggcttggaa ttaattaata aaattgtaaa tataaattta agctagtcaa
     1741 gattttttgt taatgaaaga ctagctttta tactattaat tagctgtaat tatcgagtta
     1801 gattaatact aaattttcct ttattaaagt gaaattttat gtattaattt ggtttcaaat
     1861 ttgtactaga atgaaatcaa ggaggagata caaaatgagt gtaactgaga aaattccttc
     1921 tattgaccgc aagatattta agatattgat tatgtgtagt aaaaaagagt ttgtgagtat
     1981 taactcaata gcaaatgaat tgaatgtcac aactagaagt gtaaggacgt atataaaaca
     2041 attgaataaa gatttaggta atgatatagc agtaatcaaa tatataaagg gtcaaggcta
     2101 taaactagaa ataaaagatg agcagatact caataagatt attgatatga atagaaaaaa
     2161 tgtattttca ttaaattcaa aagaagatag agtagagttt atactaaatt acttgataga
     2221 actagatgga tttattacac ttgatagttt agctgatgag atgtgtgtag gaagaactac
     2281 tttagtaaat gatttccagt atgtagaaaa agttttggct tcatataatt taaacttgat
     2341 taaaaaacag aatacaggta tgaaattaaa tggaaatgaa cttgatatta gactgtttat
     2401 attaaatcag ttgtataaaa actcaaggaa agatttcagt aattcaaagt attttaaagg
     2461 aatcaaaaaa gaagaaatca taaatttaga agaaaaacta ttgactttat ttaaaaaaaa
     2521 taatttttat gttacagatg aaatgttaag agaagttatt aactacatag tagttttagt
     2581 ctatagagta aaagaagaaa agaaagtaaa agattatgat gtaaagtttg atttacttaa
     2641 atcatatgat gaatacttta ttgctaggga aataaaacat ataatatcag gaatgtttga
     2701 atgtacttta aatgatgaag aaattattta tttaactata ccgttagtta gtggaaatgc
     2761 accagcatca gaatgtgctt taaatagtag tagaattagt aaaaacattg atgagttgat
     2821 ggaaaatata tttaatcaaa tatatgtcga tatgggtatt tttataaatg aggatgaatt
     2881 aagagtaggt ctaggatatc atcttagttt tactttaaac aggctattat ttaatataaa
     2941 gttaaaaaat gttttgttag aagagattaa gcaaaattat atacttccat ttaaattagc
     3001 acaaatagca ggaaaagtaa tagagaaaaa gtataactta gaagtttcag aagatgagat
     3061 tggatatata gcaatccatt tcagtggata tttagaaaga aatagcagta gattctattc
     3121 aataaaaaaa atagcaatta tatgtagtac aggtctagga acagcaaaac tattgaaaat
     3181 aagagtagaa aaactgatag gaaataatcc taaaattgat actctttcaa gttttaattt
     3241 gagaaatatt aacttagatg aatacgatat agtttttaca actattgatt tagacacatc
     3301 aaatataaat acaatagtat taaaaataaa tacaatattt gatgaaaaca aactaagaga
     3361 acaattaaaa acagtattgt gcctaagaga agggaatatt gacactacta attctacaaa
     3421 tttactaata aataatttgt tagatgaaga taaatttatg atattaaatg aaaagactat
     3481 acttgcctca ttagaaaaaa tgatggacca tcttatggat ttgggatgta ttgatgagaa
     3541 gtttagaaaa aatatagtca atagagaaga aaaatctccc acagtatttg ataaaggttt
     3601 attatttcct cattctataa atgaaaaatc agataagttt ttaatggcag ttggaatttt
     3661 agaagaacca atagaatatg caaatagaag tataaaaata attcttatga caatgtttcc
     3721 atgtgagaat aagatagact cagacttatt agtaaaaatt tatgaagaag ttttaaagat
     3781 aggccaagat gtaaaattaa cgaataagat aagtaaatgt agaacttttt tagaatttaa
     3841 aaaggtgtta cttaaaaacc ttatataact attgtacagc atttatagtt gttatacaga
     3901 tttaaaaata atgtgatttt tggaaggtgt ataaatatga aattattagt aattttattt
     3961 gttgcttata ttttaaatac tatatttata atgaagcaga ataaattgta ttttaaaact
     4021 ttaaatagag ctaaaaaaaa gggtgatata gtgtctacag gcaaaaagaa aagctatttt
     4081 tctaaaggaa gtatagccgt tatatcttca gattcagatg ggtttataaa gtttggagaa
     4141 attttaaaag ggcgtacagt aatggctaaa tttaaagaga ttgaagaaat agaaggatta
     4201 gatatatata gtgcagagca aaaatttaaa gatgaagaga gtattgtaca agctataaaa
     4261 tttataaaag aaaaaataaa tgtttctttt aattaactca atataaatat aaattatcag
     4321 gaggaatgtc atgaaatatg aagttaagat aagtgggata ggcagtttag ttaaagagtt
     4381 aatggatgaa agcaattgtc ttattatata tgatgaaacc attaatgatg acgacttaaa
     4441 ggatatatct gtaattcatt caatagcaac attaaaaagt gatgttgaaa taggagatac
     4501 acttactata gggaatagag attattgtat agtttcagtt ggtgatattg ctcagaagac
     4561 tcttagagaa attgggcatt gtacaatcaa atttgatgga aaatgtgaag taaatttacc
     4621 aggagaaata catgtggaaa taggaaatcc agatataact attggagatt taataactat
     4681 ttcttaattt gaaaataaaa ttattataac aggaggaatt ggtatgttag atttaaacag
     4741 aaaacttgca aaattggaag aggaaggtaa aaaaataaaa gtagctctta taggagctgg
     4801 gcatatggga aatggaatgg taagccaaat ggcaaatatg aaaggtattg aagcttctat
     4861 tgttgtagat atagatttgg agttggctca taaagctttt actgatgcag gaatagatga
     4921 agaaattata gataatgtaa caaatgaaaa ggatgcagaa ataaaattac aagaaggaaa
     4981 agtattgaca tgtaatgact ttcttgtggc ttgtaaaact aagtcaatag atgtagttat
     5041 agatgcaact gggggtattg caattggtgc agagatagca ttaaactcta ttttaaataa
     5101 aaaacatata gttatgttaa atgctgaaac tgattgtgtt gttggaccaa tacttaaaaa
     5161 attagcagat gatgctggag taatatttac aggttctgca ggagatgagc caggtgcagt
     5221 aatggaatta tttgattttg cagatgcaat ggggtttgaa gttagagtga taggtaaagg
     5281 taaaaataat aaattagatt tagattgtaa tcctgataca gttagagaag aagcagaaag
     5341 aaaaggagct tcacctcata tgatagcttc ttttaaagaa ggaactaaaa caatggtaga
     5401 aatggcttta atgtgtaatg caactggatt tgtgcctgat gtaagaggtg gtcatggtat
     5461 tgaagctaca gttaatgaag tacctaagaa atatgcatta aaaagcgaag gtggagtatt
     5521 agataattat ggtgtagttg attttgtaaa tggtatagca cctggagttt tcgtagtagt
     5581 tgcccataaa ttaaaagcag ttaatgatga actaaaatat ttaagtatgg gtgatggacc
     5641 taattacata ttatatagac catatcattt atgtagttta gagactcctt tatcagcagc
     5701 tatggcagtt ttagaccata aggctacaat agtacctaaa gctggactag tagcagaagt
     5761 tatgactata gctaagaaag atttgaaaaa aggtgagttt atggatggct atggagcata
     5821 tacatgctat ggtactatag aaaaatacga tgtagcaaag gctatgaatg ctgtacctat
     5881 tggtttgata agtaaaaaga ctaaagttgt aaaagatatt aaaaaaggtg aagttataac
     5941 ttacgatatg gtagagatag aaaaagatac tactttatat catttaagac aattgcaaga
     6001 aaaaatattt gggtaagatt tgatggtaat ataaatagat attagtaaaa agggttgatg
     6061 gttattttta aatcaaccct tttaaatttt tatatattct tgttaggata ctttctttaa
     6121 aagtatatta taggttattt gtagtcgtaa aaattatttt gaattagttc tgcaatttta
     6181 tattcacatt
//
LOCUS       DAMJYX010000150         6149 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJYX010000150 DAMJYX010000000
VERSION     DAMJYX010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.155"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(129..536)
                     /locus_tag="PC479_003277"
     CDS             complement(129..536)
                     /locus_tag="PC479_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805795.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1010..1894)
                     /locus_tag="PC479_003278"
     CDS             complement(1010..1894)
                     /locus_tag="PC479_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2805796.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2185..2661)
                     /locus_tag="PC479_003279"
     CDS             complement(2185..2661)
                     /locus_tag="PC479_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2805797.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            2927..4102
                     /locus_tag="PC479_003280"
     CDS             2927..4102
                     /locus_tag="PC479_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2805798.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4183..4986)
                     /gene="proC"
                     /locus_tag="PC479_003281"
     CDS             complement(4183..4986)
                     /gene="proC"
                     /locus_tag="PC479_003281"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2805799.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5026..5610)
                     /locus_tag="PC479_003282"
     CDS             complement(5026..5610)
                     /locus_tag="PC479_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805800.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2044 a         1035 c          689 g         2381 t
ORIGIN      
        1 ttagctttag gggtttccag caattcaagt agtattggat aggttctatc ctatctctac
       61 atataggttt ccctatatgc tgactatttt agctactaac tcatgactaa agtcacgagt
      121 gtgcgtagct attttttaat caatatttta gtaaatttta cggtaagtaa aaacctctct
      181 ccttcaaatc ctatagcaaa tatacttaca agttgctttg agaaaataac tcctaatata
      241 gctacaacta tgcatatacc aactattata tttaaaacat tgtttaaaaa ctttagtgct
      301 tgcttttcac cttgtttact acttatatct tgatacattg gaataaaagt agtaactata
      361 gcagtaccta ttgctgcaaa tataatattt ggtatattca tagctgttaa ataactctct
      421 gtatataagc ctgtaccata aattgatgat aatactaatt ctctacccat tccaagaatc
      481 ttagagatta tagtaactat catcaaataa aaagttgctt tagctacctt gctcatttta
      541 tcactccact tctaacatac ctctttttaa tcaagtaaaa tttagctcat tagatataat
      601 tctatcaact aatttgcctt gcttagccct atcaaaatat aattaacacc tatttcttaa
      661 tatatcacaa actacattat tcttcatatt tttttatttc tccaataagt tttacatttt
      721 tcctatataa attgtatagc ttcaattata ttcaaagaaa tggagttgct acccgataac
      781 tttaaaagta aaaaacagat tttattatct ttttaaacta tattctaaca tattttgtcc
      841 atattgagaa attttactaa actattatgt actttattaa ataaataaaa taacagcaca
      901 cctataaaat acaagtgtgc tgttcataaa cagattttat aaaaaaataa ttcctaagta
      961 gagattaaca tgctttctca tactctcata tataatgtgt ctaaaaatat taataaaaat
     1021 gctttaattg ttttaactta cttggatgtc ttaattttct tattgcttta gcttctatct
     1081 gtcttattcg ttctctggta actccaaata cctttccaat ctgttctagt gttttagggt
     1141 catcatcatc aattccaaat ctcattctta aaacttgctg ttcttgctct ccaagacctg
     1201 taagtagttc ttctatcttt tcttttaaat tatgctgttc aacttcgtgt attacaacat
     1261 ctttaaaatc agcatctgat ggtataaatt caactaaact actatcctca tcttctttta
     1321 atggagtatc caaagataca acatttttat ctcttcttaa taattccaac actttgtcaa
     1381 cttctaagcc acatgcatct gcaatctcat ctatagttgg ctctcttaat aatacattta
     1441 aaagttcttg tttctttttc tttacaaaat ttattctctg atgaagatgt ataggtatcc
     1501 taatagtatt ttcgcaatcg tcgatatatc ttgttatact ttgttttatc caccaagttg
     1561 cataagtgct aaatttgtat ccttttttat aatcgtactt ttctacagct tttataagtc
     1621 ctatatttcc tgcttgaatt aaatccaaca tatcaatact atctctttta tatctctttg
     1681 ctatgctcac taccaaccta taatttgaat ttataaattt ttcttttgct actgaagatg
     1741 agttgattat ttccttagcc agctctacct cttctcctgc tgacaacatt ttgtattcct
     1801 ctatttcctt taaatacatt ttcatcgcat tcgatacatt tgatgaaccc acacaaatat
     1861 aattttcata attattttct tttttagtat ccataatcaa accccttcta ttcatagaat
     1921 ttgctaaaac aaaaataaat aatttaaacc tacataatct acaattacat aatatcccat
     1981 tctttaaatg aattgttttg tatattatgt atacaaattt ataaaaacta tacctataat
     2041 attattctac ttctaacagc aatatcctcc ttatatatat aatttatttt attatatgtt
     2101 gttatattct tagttttttg aatgatatat gtctaaaaaa agactgttta ctaaacagtc
     2161 tttttttata tatatgctca atatttaatt ttcatgatga tgattacatc cacatccaca
     2221 attttcatca tggtgatgat gttcttctaa atcttttacc tgttgttcca tttcatgaaa
     2281 ctcttttacc attatatcat aagttggaag agctgcctca gtatcgtatt cttcccctct
     2341 ccaatcagcg tacatcatag catctccatt agttaaaacc aatctacctg acatagttat
     2401 attatccact tcttctaaaa attctatttg tttttgtaaa acttctcctt ctaactccac
     2461 atcttcatct aaacattgca ctatcattac aggtgcataa taatcttctt catctttaac
     2521 atttacaaca atatctaaaa ttccttctgc accttcttca aattctgcca actcaacatt
     2581 tgtatcaatc atatcttgag gtactaataa atcttctatt aaatatttta ttactctatc
     2641 ttttactaat tcttttgaca tttatttttc tccttccgat tctataacat tatcataatc
     2701 atttctatat tatagtatac tacattaatt gttttttcaa tctcttacaa aaattatttt
     2761 ttattatctt ccttatattc ccttttttat tacaaagttg taatcgcatt gatttaaaaa
     2821 atagtttata tttagtataa tagaaatgta gcacatattt ttattcaata tattaataaa
     2881 aaaatatatt gaaaatgtaa tgtatgttaa atctaggagg aatattatga agtttaaaaa
     2941 acaaattagt tctattgttg tgtctactat gcttgtactt gggagtatga acctttctta
     3001 tgcagacggt acaaatgtag ttacacttgg agctaactta tcagagtctc aaaagcagca
     3061 aatgcttaat tactttggag taaaaaaaga ccaagtctta gttttagatg taacaaatgc
     3121 agaagaaaga cagtatttac aaggtgttgc aacagagggt caacttggaa aagttactat
     3181 ctcttgctca tacgttgaac ctactaaaaa aggaaatgga attaatgtta aaacagcaaa
     3241 cttaacttgg gtaactagtt caatgattgc ttcaaccctt acaacagctg gtcttgaaga
     3301 tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt actggagcat tgacaggaat
     3361 catgaaagca tttgaggatg cttcaggaaa gaaacttgat gaaactaaga aagaaattgc
     3421 ttctgaagaa ttggtagtta caggtgactt aggagatgat atagggcaag aaaaagcaac
     3481 aggtgtaatg aatgatataa aaacagaaat tgtaaagaat ggtacaaaag ataccaatca
     3541 aatagcaaat acaataaata atgttgtaaa taattacaat attactatta caccagaaca
     3601 aaaagaacaa attaaaggtg taatgaaaaa aatagctgac caaaattacg attacaatag
     3661 catgaaaaat acactagata atgtatcaga taatgtaaat gaacaattaa agaaattagg
     3721 tgaaagtgtt aagggttctg gtatactaga tactattggt ggatggttta gtggaatagg
     3781 agactggttc tctggtttat tctcaggcgg agataaaaaa gatttgggaa tcttaaataa
     3841 tactaatgat gagtctcttg gctctgatgc tgtaataaat tctacagaag gtgttcaaat
     3901 aaatccacaa aataatacta attctaacaa tgatagtgat acaaatagct caaaaaatga
     3961 tacttcaagt aataatgaaa ataacgatag ttctcaaaca aatgaaaaca acacaacaaa
     4021 taattcagaa aatcaatcaa ataataatga ctcagttggt ttctttgaaa aaataaaaaa
     4081 ttggctttca ggcttatttt agaaattaaa caaataaaaa gaattgccta aaatggaaat
     4141 tttctataaa aggcaattct ctttatttac aataaatttt tattactcac tcattttttt
     4201 agatttttct atacatgcac atattgcttt tatcacagaa gctctaaaac cttcttcttc
     4261 aagtacttta actgcttcta tagtagttcc accaggtgaa cataccatat cttttaattc
     4321 accaggatgt tttcctgtct caagtaccat ttttgcagaa cccataacag cctgtgatgc
     4381 aaacttatat gcttgtggtc ttggcattcc tgcaattact gctgcatctg ctatagcttc
     4441 tataaataaa aatacatatg ctggtgcaga accacttgca ccaattacag cttctataag
     4501 atactctggt actacctcag tatttccaaa agaattaaat acttctgtaa ctgcttctaa
     4561 gtcttctttg tttatatttt tatttatcga aagagaagac atggcttcat ttacaagagc
     4621 tggagtattt ggcatagttc taactatttt tttatcttct cctataatag actctatatc
     4681 ttttatagat tttcctgctg ctattgtaac tataatttta ttgtcatcta taaagtcttt
     4741 tatctcttcc aaaatatcat catatatatc tggtttaaca gctacaatta ctatatcaga
     4801 ggttctagtt acttccttcg agtcttttgt agtatttatt ccaaaatttg catctattct
     4861 atcgagagtt ccttgactat atgctgatgc tataaccata ttaggttcaa ctaattttga
     4921 atttactatt ccccctatca tagcgcttgc catatttcct gcacctatga atccaatttt
     4981 tttcacttta gtacctcctt attgactaat agtaatcgtt aaaatttaag cttctttaac
     5041 tgcatcagat ttttcaaatg gatttggtat acttactacc aaaattttca ctaccttatt
     5101 agtatagttg acccaattat gattgttctt ttcactatta tagtgtattg tgtctcctgg
     5161 atacatttct atttgttcat ctcccaaaaa cactgtaaga gtaccctcta atacatacac
     5221 aaattcctca ccttcatgta cgtaacaaca aatttcctca ctactcttac ttggaagaat
     5281 ttcaattaat cttggtagca ttgttttttc ctttaaacta gatgataaat gataatgtat
     5341 aaatgttgag ttttctacat caaatacttc tttttcataa cttcttaata cagctctttt
     5401 atgctctttt ggcttcataa aaaagtaagt taattctaca tctagtacac ttgctatttt
     5461 tcctaaggaa tctgttgcca cacttgttaa tcctctttca agttgtgata aaaaaccaat
     5521 tgacaagttt gttttttcac tcatatcctt cagcgtcatt tgcttttgag ttctgagttg
     5581 ctttattttt gctcctatgt ccttattcaa aaccatcacc cctaaataat tttaattttt
     5641 tcataatata aatacaaaaa tataaatata ggtactgaac ccatctttat ttcatttaaa
     5701 aaaattttta taagtattac tataatttat caatcttttt ttgtctacat ttttacgatt
     5761 atttacgatt tatttttcac atatgtatat ttaatcttgc caactaaaga tacatttttt
     5821 ataagcattt actctttata taataaaact taagttgttc atgtagtctt cttctggttt
     5881 aattattaaa atttcaaatt atattattat ttctacacat tattacatcc tttagtacaa
     5941 ttataacata attttacaat ttaatgatat ctattcaatg tcttttttta ctctacaaca
     6001 tattttattt tagagtttaa tactatatat attttttata tacagccttg tcatttttta
     6061 gattgtcaag agttattgaa gtattttatg tagatttaga ttttatttcc taatttctca
     6121 tttaacaaga attgcttatg ataaaaaat
//
LOCUS       DAMJYX010000151         6094 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJYX010000151 DAMJYX010000000
VERSION     DAMJYX010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.156"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            72..1673
                     /locus_tag="PC479_003283"
     CDS             72..1673
                     /locus_tag="PC479_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2805801.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1892..3490
                     /locus_tag="PC479_003284"
     CDS             1892..3490
                     /locus_tag="PC479_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2805802.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            3783..5963
                     /gene="slpA"
                     /locus_tag="PC479_003285"
     CDS             3783..5963
                     /gene="slpA"
                     /locus_tag="PC479_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2805803.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
BASE COUNT         2445 a          644 c         1209 g         1796 t
ORIGIN      
        1 ggaaaataaa gagaaaataa atggaaaata atcacaatat aaatataaaa tataaaaatc
       61 atcaggggga tatgaaaatg aacaaaaaaa tattatcatt aggtctagca gtatcattaa
      121 tcttagtaaa cttcaaaagt gtaaatgcat catcagtagt agaaaaaata tacggtaaag
      181 atagatatga aacagcagca aagatagctg ataaacagac ttatgaaaca gtaattttag
      241 taaatacaga aaaatcactt gcagatggat taagtgcaag tggattatcg ggagctacaa
      301 aagctccaat actatttaca caacaaaata agataccagc agacacaaat agatgtctaa
      361 aaaatatcaa aaaagcatat ataataggta cagaagatac tataagtaaa tcagtagaaa
      421 aagaactaga ttctaaaaat atagaagtca aaagaattgg tggagaagat agactaaaaa
      481 caagctattt aatagctaaa gaaatagcaa ctataaaaaa agttgataaa gtactactaa
      541 ctaatgcata tagtggagaa gcagatgcaa tgagtgtatc atcagtagct actagagatg
      601 gagctccaat catacttaca gatggaaaga gtgtgccttt tgatgtaaaa aatattcaat
      661 catattgtat aggttcagag gagataatga gtaatcctct agttaaaaat acaaactcag
      721 taagaataga aggaactgac cgatttgaaa ctaacaaaaa cgtaatagac tactttttta
      781 atagtgcaga tggattctat gtatcagatg gataccaatt agtagatgca atagcagctg
      841 caccacttac taaaaactct ccaatggtac tagtaaatga tggaagcgat aaaactgtat
      901 tagaaggagc taagaatata acctctgtag gtgaaataga tgagaaggta atacaacaat
      961 gtataagtgc ttcaaagtca aatggacaac ccccagcaat tacagttgga agtacagagg
     1021 tatataaggg tgaaaagttt gacactggca agttaaatat agtagctaaa gataacactg
     1081 ggaaggtatt accaatagaa gttgatgggt ttatagatac aaatagagta ggtacataca
     1141 tattgacatt aaaagctaca gacgaatggg gaaaaagtac aggaaaaaga gtagaaataa
     1201 aagtgttgga tgataaatct catgattaca acagcccaga atttaaaaaa atggtatcta
     1261 ctgaaatgta taacttaatc aattcctata gaaaagaaaa aggtaaagag cctttggtag
     1321 tatctagtaa gctagaaggt atggcaaatg catggtctaa gtatatgatg gataacaaag
     1381 tttttgaaca ttatatagat ggcaaaaatg ctccacaagt attttctgag tttggaatga
     1441 gaagtgagga aaatatagca tatatttata tagattctaa gagtgttcaa acaactcaag
     1501 atgcaaaaga cttggcaaag gctatatttg aagtgtggaa gaaatctcca gaatacaatg
     1561 caaacatgct tagtgatgag ttttatagta ctggatttgg gctttatata ttatctgatg
     1621 gtcaagtgca tgctactcaa gagtttttaa atggcaatga aggtagcttg taaaaaattc
     1681 cataaatctt gtttttttgt taatttactg atattataaa atgaaaatca catctaaagt
     1741 actttttgta atctgtttta ttattgcttt acaggttatg gtattttagg tgttttttgt
     1801 tttagtagtt tataaatttt gagggaatca ctactgaggg tgtaatttat gattagctga
     1861 attatataaa aaaattagta ggaggagttc gatgaaggta aataagagag tattatcaat
     1921 aggattgaca atatcattaa taatggcagg agcaccaaat atcaatgcac tttcgagtat
     1981 agaaaagata caagggaaag atagatatga gacttctgca ttgatagcag ataaacaaat
     2041 atatgataca gtcatattag taaatactga taattctata gtagatggat taagtgcaag
     2101 tggattatct ggagttgtaa aagctcctat aatgttagtg caaagagata agataccaac
     2161 agatgttgaa aaaagactaa aggatgttaa gaatgcttat gttataggta cagaggatac
     2221 aataggtaaa tctgtagaaa atcaacttaa gaataaaggg attgaagtta aaagaattgg
     2281 tggggaagat agaataaaga ctagctatct tatagctaag gagatttcag ctattaaacc
     2341 agtaaatgat ggtgataagg tttttcttgt aaatggatac acaggtgagg ctgatgcaat
     2401 gagtgtatct tctgtagctg ctagagatgg agttcctgtt atacttactg atggaaagag
     2461 tataccattt aaggttgatg gcgttcaatg ttattctctt gggtcagagg agataatgag
     2521 taatgaactt gtgagtaaga ctaattctgt taggattgct ggtaaagata gatttgaaac
     2581 taataagaag gttatacaac gcttttataa aggaacaaag aagttttatg tttctcaagg
     2641 gtataagtta gttgatgctg tggctggttc tccgttggct aaggacaaac ctatagtact
     2701 tgttaatgat ggaagtgata agagtgtgct tagaggtgct gatgaagtta cttctttagg
     2761 tggaatggat aagaaggttg ctgaccaatg tattagttct gcatctgata aaaatacaat
     2821 gcctacaatc actgctaatg atgtagagat ttctgtaggg gataagtttg ataatagtat
     2881 gttgaatata gttgccactg attattatgg aaataatttg aagcctaata ttgagggaac
     2941 tgtggatacc agtaaggcag gaacgtatgt tttaaatgta agtgcttttg ataattttgg
     3001 gcaaaagtgt gagattaatg taaatgtcaa agtaattata aacacaagta caaaagaccc
     3061 taatagttat gagtttaagg ctatggtatc taatgaaatg tataatctag ttaattcata
     3121 tagaaaagaa aaaggtaaga aaagtttaag agaattagat tctttagctg gaatggcaaa
     3181 tgcatggtct aagtatatgg atgagaagaa gatatttgct catgaaatag atgggaaaaa
     3241 tgcagctgaa gtattttctg gctttggaat gagaactgat gaaaatattg catatttacc
     3301 tatgaatgtt aagtctgtat atacatctaa agatgcaaaa gagattgcta agtctgtatt
     3361 tgatttatgg aagaagtctt ctaagtataa tgataatatg ttaaaggaag agttttatag
     3421 ttttggcttt ggaatgtatg tttctaatca aagagaagtt aatgctacaa tggaattttt
     3481 aaatagttag agtatatatt aatattttat ggtagaaaat attaatatat attgattaaa
     3541 tcagataaat aaatgtatta ataaaattaa aattgaaaat ttaatttaaa aaatatgaaa
     3601 tagcaaaaaa aaggggaaaa atgttgaaag aaaattctat taattttgca taaattattg
     3661 aaattctatt ttatatagtc tatcataaga gtgtgagaaa tatttggttt atatgaacat
     3721 ggaattttaa tgtacataat taaagagatg taattaatat aatgttggga ggaatttaag
     3781 gaatgaataa gaaaaatttg gcagttgtaa tgtctgccgt tacagtaatc ggatcagcag
     3841 caccagtttt cgctgcagca gaggatagtt ttccaaatgg aaataccact gtatctagta
     3901 gtaagtatag tgatatagaa agaatactta aaaaatatgt tgcagatggt gtaacaggca
     3961 taacagttaa cttttttgac aaaaaaggaa atccaaaagg ggtatctata tcaagtttag
     4021 aagcaagtca aataagttat gttaaagatg aaattaaaga tttaaaagct ggagaatatg
     4081 caaaaatatc tgtaaaaatt acagatggaa cagaaaaaaa atatgatgat acattattaa
     4141 aggaacttga tagtaatgct gtagttacta aagttggtac tgcaacaaca gttaagattg
     4201 tagatcaaga tggaaattca atagaaaatg atgctaaatt aaagttacaa aaatatgata
     4261 caactacgtc agcttatgta gatggagcta ctaaagatga agttttagca gctacaaagg
     4321 cagcagtcaa aacttcaaaa tttactgata agactggtaa taatgctaca ggaacagtag
     4381 ctattacagc aacatatgct gatgcagtag ctggtgcaat taaattaaaa tatgatacac
     4441 aagcagcagt agcacctact ttagatacag cagtagcatc tcttgatggt actaaagaga
     4501 tatatgattt tagtaaacca gttataacgg ttaatactac tgatggtaca tcaaaattaa
     4561 gttttgaaaa aactggagaa aaagttggag cagttgaatc aaatgtaaca gttatagcag
     4621 catcagatga aacagttaca atatctggag atgcaaaaga aaaagcagaa gctttagcta
     4681 aaaaatatgt atttaaagat actgaattag aagatgcata taagacagta acagcaagtg
     4741 attttgaaaa aactgacaat gattattatg aggtagttct ttatccaaca ggtaaaagat
     4801 taaatacagc ttcaacatat gcatcatcta actataagcc agaattacct acaagtgata
     4861 gagtagatac tcctgctata ataacattaa gatctactaa taagaataac ttaaaatcag
     4921 ctttagatga attaagaact tataataatt cttattcaaa taattctgta ttagctggtg
     4981 atgatagaat agagactgca atagaaataa gtaaagactc ttataatgca gatggaggaa
     5041 ttaaaggaga ttatgtagaa gctaatgagg tagtattagt aggatcacaa tcaatagtag
     5101 atggtctagt agcttcacct ttagctgctg ttaaagatgc tccattatta ttaacttcaa
     5161 aagataagtt agattcatca gtaaaatctg aaataaagag agtaatggga ttagatgata
     5221 aaactggtat aacatctaaa aagactgttt atatagctgg tggggaaaat tctgtatcta
     5281 aagaagtagc aaatgaatta aaagatatgg gattaaaagt tgaaagatta tctggtgatg
     5341 atagatatgc tacatcttta gaaattgctg atgaaatagg tttaaaccat aataaagtat
     5401 ttgtagttgg tggtactgga ttagcagatg caatgagtat agcatcagtt gcttctaata
     5461 aagaaatgcc aatagtagta gttgatggaa aaggaaaaga tttatcaact gatgcaaaag
     5521 actttatagg aagtgcttat gtagatataa taggtggaaa atctagtgtt tctgaagaca
     5581 tggaagatgc tatagatgat gctacaggta agagtccaga aagagttagt ggagatgata
     5641 gacaagatac taatgcagaa gttataaaaa cttattttga aaaagataat agtgacagtg
     5701 taattagcac aggtgttaaa aacttctatg ttgctaaaga tggttctaca aaagaagatc
     5761 aattagtaga tgcgttagca atagcagcag tagcaggtca caatgaagct ccaatagttt
     5821 tagcaactga tagtttatct tctgaccaat cagtagcaat aagtaaagtt acaaattctg
     5881 atgattctaa gaaattgact caagttggta aaggaatagc agattcagtt attaagagaa
     5941 taaaagattt attagaatta taagataaaa ttaaactata aataagaggg aacttctttg
     6001 aagaagttct cttttttata aaaagaaatt aaaaaaggaa ctaaaaatag ttcctttttt
     6061 tgtgagaaat acctaaataa ttaaagatgt aatt
//
LOCUS       DAMJYX010000152         6062 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJYX010000152 DAMJYX010000000
VERSION     DAMJYX010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.157"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(1027..1578)
                     /locus_tag="PC479_003286"
     CDS             complement(1027..1578)
                     /locus_tag="PC479_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805804.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1769..2731
                     /locus_tag="PC479_003287"
     CDS             1769..2731
                     /locus_tag="PC479_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2805805.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            3026..4870
                     /locus_tag="PC479_003288"
     CDS             3026..4870
                     /locus_tag="PC479_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2805806.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5192..5776
                     /locus_tag="PC479_003289"
     CDS             5192..5776
                     /locus_tag="PC479_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2805807.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2375 a          593 c          930 g         2164 t
ORIGIN      
        1 taaaaaataa ttaccttata aatagattga aatttatgaa tattcatact ataatttaaa
       61 tataaggaga tgccctttga aaataaaaat taaaaaatat ttaatgctac tacaatagga
      121 actagaacta cacttaataa atatacagaa attagatttg gctcaatata aaatacaaat
      181 atagaattta ggtgtttttt atgaaaaaat ttttatatgc tttatatagt ttcattgtta
      241 ttatagctaa ttttagatta aaagaaaaaa attataactt tattttatta gcaatctttt
      301 ttatattgct gttaattcaa aatggagttt taaaaacagg ttatcaaaat agagctacat
      361 tttatcaaga taattatttt aatagagagg ataaaaatga taagataaaa tttatgacta
      421 taaagatttt tatatttttg tcattaccat tatgttttaa tatactattc aattatttaa
      481 cctcttaaat tatatttaga tataggagtc ttatgataaa agtaagctta tgtgttattt
      541 gtagtaaata taaagtgcat atagtgataa aacaaaagtt tcatataaat gaagaaaaaa
      601 atataaaata atttatttaa ctacttaaaa ataaactaaa agggataata aaatagcttt
      661 taggttatgg taataaaggt atttaaaaag agtgcataag cactccaatt gtgagaaata
      721 catacacaaa tcattattac aacaatgaaa aatgtacata taagagacgt tttgtatttg
      781 ttttaagtga taatatttta atactaacta aataattatc acaatagtaa aaaagaaaaa
      841 ggagtatttt gtactccatt tgtcaaaaat ataaaacttt tatataagct tattataaca
      901 taattgataa aagtgtaaag atatataatg aatttataaa gaaaaataaa ataaaaaaga
      961 taaatcctaa tcaaaggatt tatctttttg tatagaaaat aattattcta ttattaaatg
     1021 gcatggctaa actggataat aatttattat tactgctttc aatgtttttt ctccttgatt
     1081 atggtatgta tgaattgatt cagctgaaaa acaaatagag tcattttctc taagttgata
     1141 aatagagtca tttacttcta attttaattg accttctata atcataatat attcctgtga
     1201 tttttcagaa tgaccaacag atgtataact atgaccttct tctaattcca tttgaaataa
     1261 ttcgaagttt ctagttggtg tattgggata ataacaataa agacgataat ctttattttc
     1321 tgaaacttgt acatctatat cagtttttga tacaataaaa gtttcatttt gaggttgttc
     1381 cagtattgca gtatatggaa catttagtcc acttgcaatt ttccaaattg tgtttactgt
     1441 tgggtttgag tctccctttt caatttgcga aagcattact ttacttacgc cagataattc
     1501 agcaagttga cctagactta agttacgttc attacgtaat ctatttaaat ttttagcaat
     1561 aacagtatta atactcatag tagctcctta aaatgtaaat tattgacaaa tataatttac
     1621 gaatgtataa tttatttaac attatttata atttacaaat gtataatata atgaacgtta
     1681 aatacactgt acatccgtaa tataaattat actgcataaa gagttgtttt tcaacggatg
     1741 atagttgaga gattaagggg gaattgtaat gcgtgtactt aataatgtta cagaggctat
     1801 aggcaatact catatgttac atttatccaa aatagcagat gaatttggag ttcaaggtaa
     1861 tatttatgca aagatggaat acttaaatcc gggatttagt aaaaaggacc gtgtagcatt
     1921 acaaatgatt accgaagcag aaaatgcagg tttattagtt ccaggtcaag cagttgtgga
     1981 attaacaagt ggtaatactg gaacaggact agcaatagct tgtgcatgta aaggatataa
     2041 atttatagcg tgtatgtcaa aaggaaattc tatggaaaga gcaagaatga tgaaagcttt
     2101 aggtgctgaa gtagttcttg tagaccaaat gcctaattca gttcatggac aggtatctgg
     2161 tgaagatttg gcacttgtag aaattgaggc tcaaaaaata gcaaaagaaa gaaatgcatt
     2221 tagagcagac caatttaatt tagaagcttg taatcatgca catgaaaagt atacagcaga
     2281 agaaatgtgg gaacaattgg atggaaatat tgatgtattt gtagattttc ttggaagtgg
     2341 tagtactttt gcaggatgtt caaaaacact taaaaaacat aattcttcta tcaaatgtta
     2401 tgttgttgaa cctgaaacag cagcaattta ttcaggtaaa gaaataactg accaagggca
     2461 taaaatacaa ggtggaggat attctatgga tttgccatta gtagacaaga aacttattga
     2521 tggttatatt caagttacag acgaggaggc aactgatgta gcaagaaaat tagctaaatt
     2581 agaaggtgta tttgcaggat tttcttcagg ggcaaatgta tgtgcagcaa ttaagttgtt
     2641 aaagagcagt gaaaaaggaa aaaatatagt tttaactctt aatgatagtg gtttaaaata
     2701 tttaagtact gatttatatg aagaattgta ataatagcac aatagttatg caatgttttt
     2761 taaattaagt atatagattt aaattataaa aagtatctca atgtaatttt tgaaagttat
     2821 aaaggagata cttttttatt ttgtaaatat ggagtatcct acttataagt attaagctat
     2881 atagatattg atgattagct atgcaataaa aaagagtatg tgtttttgca tttatacatc
     2941 ataacttgga ataatacata ttaatgttct aaatgtattt aaaacatatg ataaaattag
     3001 aacaaataga tacaaggaga taaaaatgca aaagagaatt ttaagaaatt ttatatttac
     3061 gggtaaaaat attttaagta aacttgtttt tataataaaa tataatttta gtacattaat
     3121 tttatttgaa ttatttcata aaggattagc cttatttttt atatggccat ctattagata
     3181 tatatttgat attttaataa aaagtttaca tatagtctac ctaaatatgg ataattttat
     3241 aaaagttttg accaatccca tgtcaatagt tctgataata ctatcagtta taatactagc
     3301 tttttatgca ttttttgaat ttacctctgt aattatttgt tttaataaat cgataaaata
     3361 tgaaaaaata gggttatttg aactctttaa aatatcattt aagaactcta taaaattgtt
     3421 atatcctaaa aatatactat tatttatatt tgtgatatta ataattccat tagtaaatac
     3481 agttttaatt tcagggttta taggaaaaat acaaatacca gagtacatac tagattatat
     3541 aaagtctgat ataattttaa atataatcta tatgattttt cttttaataa tatatatttc
     3601 ggttattaga tggattttta gcattcatga gataacttta aatacagaaa attttaaaag
     3661 agcaagaaaa gaaagtgtta atcttataaa gggaaagatt ataagaacaa ttatttattc
     3721 attaatatta tctttagtag tagctattat tggatatgcg atttatcata gtggaattat
     3781 atttataggc atatggacta aatattgtac tgatatggaa tttttgaaga atgtatttat
     3841 aaatagatgt attgcatttg aagaatattt tagattgatt ttatcaatat tcatttttat
     3901 attggacatt ggatttatct cagcaactta ttatcaatat aaaggagtaa atatttctaa
     3961 aaataatatt aaaaaagaga aaaaatctat atcaaaaatt gtttttaatt tagtattaat
     4021 attattgatt tcttatatag agtcagttgc attttcttca catataaatg gtatgtttaa
     4081 tacgtcattt tcattttata caacagcaat tgctcataga gcaggagctg aaatggctcc
     4141 agagaatact ttggcagcaa tagaagaagc ttcacatgct aaagctgatt atgcagaaat
     4201 agatgttcaa gaaactaaag atggagaact tattgtactt catgattcaa actttaagag
     4261 aactacagga gtagataaaa atgtttggga tgtaaattat aatgaagtaa agacatatga
     4321 tgcgggaagc ttttattatt atggaaaagg ttattatgat gaaaagtttg ttggggaaaa
     4381 gataccaaca ctcaaagaaa tgataaaata ttctaaaggt aaggtgaaat tattaataga
     4441 aataaaactt aatggacatg aaaagggaga cgtagtaaaa aaagttgttc agttaattaa
     4501 agaaaataat tttgaaaatc aatgtatagt tgcatctatg gataaaacta tattacaaaa
     4561 agtaaaaaaa ctggatgaga atttattgac atgctatttt atggcaattg catatggtga
     4621 tttttcaaag ttaaattatg ttgatatata tggaattgaa tcaacttttg ttaataaaac
     4681 agtagtagaa aagattcata aaatgaacaa aaaaatcttc gtttggactg ttaatagtga
     4741 cagtttaatt gagaaaatgc ttgacctaaa tgtagatagt attattacag ataatccata
     4801 ccttatagag agtgcaattt attggaagaa aaatggattt attagtcaag tgtcagatta
     4861 tttattttaa atattctata attgtattat ccagtaaaag agatattatt aaaatcaata
     4921 atatctcttt ttaacataaa taaggttgtt aaattaaggt tattcttata aaaattgtgc
     4981 ttatcagcta taaacaagga ataaaagtga aaaaattgct ctgagattat tacagttaaa
     5041 agagttttaa gatgacagag agatagattg tcaactgtat atttaataaa tcttgttatt
     5101 tattaaattt tatataagta aaatatataa attaataaat cttatgctag aatatatatg
     5161 aaaaacaata taaatttgga ggattaaaaa catgacagag cgagaaaaaa tgttggcagg
     5221 agaactttac gattgtggtg atattgagtt gttaactcaa tggcataaag caaaaggcct
     5281 agtaagagat tataaccaaa cagattcctc ggatttggaa aaaaaagaaa aaatattaaa
     5341 tgagttactt ggtggtaagg gtaaaaactt atggattact gcaccgtatt atgtagatta
     5401 tggaaataat atttattttg gaaataattg tgaagtcaat atgaattgta cgtttttaga
     5461 tgataataaa attatcattg gggataatgc actaatcgca ccaaacgtac aaatatatac
     5521 tgcatttcat ccaactaatg ctggtgatag atttggtaat gccaaagaag atggttcatt
     5581 tgaattttgt aaaactcaaa cagctccagt tactattgga gataatgttt ggattggcgg
     5641 gggtgctatt atcatgccag gtgttactat tggagataat gtagttatag gtgcaggaag
     5701 tattgttaca aaagatattc caagtaatat gattgcatac ggaaatccat gccgtattat
     5761 tcgagaaaat aaataaagtt aaattttctt gcaaaatctc aaaaaacacc tttcttactt
     5821 tatacatagg gtattttcta atactttcac atccatattc atttgttaaa tttttgtgga
     5881 atgtagtttt tatcacttct atcttacatt tccaccattt ttttataaaa gtcattcctt
     5941 tatagattta tttataatag tatatattct atttgatgtt tgaataagca cttattttta
     6001 agtaaaatac taacttttag gttagtattt tgctatagta tgatgattaa gtaagaataa
     6061 aa
//
LOCUS       DAMJYX010000153         6060 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJYX010000153 DAMJYX010000000
VERSION     DAMJYX010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.158"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            94..1419
                     /gene="cdeA"
                     /locus_tag="PC479_003290"
     CDS             94..1419
                     /gene="cdeA"
                     /locus_tag="PC479_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2805808.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1514..2308)
                     /locus_tag="PC479_003291"
     CDS             complement(1514..2308)
                     /locus_tag="PC479_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805809.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2309..3184)
                     /locus_tag="PC479_003292"
     CDS             complement(2309..3184)
                     /locus_tag="PC479_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805810.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3188..3556)
                     /locus_tag="PC479_003293"
     CDS             complement(3188..3556)
                     /locus_tag="PC479_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805811.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3739..4398)
                     /gene="deoC"
                     /locus_tag="PC479_003294"
     CDS             complement(3739..4398)
                     /gene="deoC"
                     /locus_tag="PC479_003294"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2805812.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4434..5621)
                     /gene="murJ"
                     /locus_tag="PC479_003295"
     CDS             complement(4434..5621)
                     /gene="murJ"
                     /locus_tag="PC479_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2805813.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5698..>6060)
                     /locus_tag="PC479_003296"
     CDS             complement(5698..>6060)
                     /locus_tag="PC479_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2805814.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         2005 a          959 c          671 g         2425 t
ORIGIN      
        1 taagctgttt atcgtataaa taaaattttt cttgactata tagttactat atagcttatt
       61 atatatagag agtaataaaa ggaggtaaaa tttatggaaa atttatttac aagaaaattc
      121 actacttttg aatttctaaa atttgtttct cctgcaatta tatccatgat atttatatct
      181 ttgtacacaa taatagatgg catctttgta tcgacattag ttggttctga tgctcttgct
      241 agtataaata ttgtactacc tataattaac cttgtttgtg gatttggaat aatgatggca
      301 actggtggag gtgctatcgt ttctatacgt atgggtgaaa atagacagga tgaagccaac
      361 tctacatttt cttttatagt tttgttttca ttgattgttg ggattttatt cacagtaatc
      421 tcatatttct ttatcaaaga aatatctatt ttgcttggtg caacagataa gttattacca
      481 tattgtataa cttatggtaa agttatgatt ttatgtacac cattttatat tttaaaattt
      541 atatttgagt actttgcaag aactgatgga aattctaaat ttagtttatt tctatcagtc
      601 attggtggtg taacaaatat aatattggat tatgtattta ttaaatattt tggaatgggt
      661 cttttaggag ctgcagttgc aactgctata ggtattattt taacttgtgt tttaggtatt
      721 atttacttct tatctaataa atctacacta aaattaagaa aaccaaaaac cgattttaga
      781 cttataagag atactatgat aaatggttct tctgaaatgg ttacagaatt atctacagga
      841 attacaacat tcttatttaa tgtagtagct ttaaaattag caggagaaaa tggacttgct
      901 gctcttacta tagtattgta tgctcatttt ttaatgacat cagtctatct aggattcgct
      961 gctggagtgt ctccattaat aagctataat tttggtgctg aaaacagtga taaattaaaa
     1021 gaaacattta aacattctct aaaatttata tttgtttctt ctcttttagt gtttattttt
     1081 gctttagtat ttgcaccatt tattgttagg gtctttgtaa gtccagataa cacagtattt
     1141 aaactagcct tacaaggatt aaaaatattt gcatttgctt ttttgtttgt tggtataaat
     1201 atatttgcat caggattttt tacagcattt cacaatggaa aaatttcagc tattatatct
     1261 tttagtcgtg cctttgtttt tataatcata ggaatcataa ttcttcctcc tatgttaaac
     1321 atgactggat tatggcttac agttccattt gctgaggtta taaccatatt tatatctatt
     1381 ctatttataa aaaaatataa atgtagatat aagtattaaa aattaatact taaaatcaac
     1441 atttattaaa actaataaac ctgctttaaa atacaaattt acaaatttga tttttaagca
     1501 ggttttattt tagttataat aagtcatttg catactcttt aattggtgct tttctaagta
     1561 ataaaattat aaatattatg caaataacta taattcctac ttttgttcca aataaaataa
     1621 aaggattctg aaatgaataa ataatttttt ctaaatacaa tagcatttta tgcatatttt
     1681 tagttgcaaa attaactata taaattatca ttaatatcgg taatcctatc aatatagtaa
     1741 tactagtaac ttttctaact ctatatgtaa aagctccaag taacactcct ataaaaccta
     1801 tacatatttc atttatacat atttgtacaa aataatttaa ataagttagt ggcatacttg
     1861 atgttgaagg atctatcagt gctgatatag atgtccaaat catatctctc tttagtaaaa
     1921 ctgatagttc tatactgtaa cctgttataa tttctataaa taacttcgat aaaaaaatca
     1981 aaactatacc taaaatagaa atcacaaagc ttaaaataat taaatctatg acaatagctt
     2041 ttatacaact ttttctatct gctcttatac ttaaagctcc tgaaaagtct ttatttgcta
     2101 tatttactcc atacacaaat agcagtatag ttatatatgt taaaatagga ttcacaatcg
     2161 tggataaatt agttttagat gcttctccaa aaatcttgag aataaacact ggcaaaatat
     2221 tcataacaat tcccacaatc ataaatataa ttatatacaa tttagtattt ttattaaaaa
     2281 attttatata tggctttaat tcattcaatt acaattcctc cttcttagtc atgttaacaa
     2341 acatatcttg tagccctatg tatcctacat caatatttga atttttaagt attttttcat
     2401 cactttcact aaaatcacca tagtaaaaat aagcaactgc atttccaaat gcctttttag
     2461 gttttatatt ttttatacat tctaattttt ctaactcttc tttatttcca gtaatataat
     2521 gagctttttg ttttatgaca tcaatttctt catctataat aatttttccc tcatttaata
     2581 ttattacatg ctccagtaaa tcatctattt catcaatcaa atgagttgat attattattg
     2641 tccttggatt ttttatataa ctatctaaaa ttatgttata aaactcttgt ctatttactg
     2701 catccagacc aattgttggc tcatcaaata tggtgatagc tgaattagaa caaataccta
     2761 ttatatttga aagtatagtt ttcatacctc ttgacagttg tttatacttt tttttagtat
     2821 ttaaatcaaa atatttgcat aattcttctt ctaagccttt atcataactt ttataaaaac
     2881 tagaagaaaa ttcaaatatc tgacctactt taaaatctgg actataaaat tctttttccc
     2941 taacgataca aacttcttcc aaaactttta tatcttcttt tgggttcttt cctaatattt
     3001 ctatctcacc ttcattttga attaactgat ttaccattat atttaataat gttgtttttc
     3061 cagctccatt cttacctaat aaaccatata tcttattttt ttcaaagctt agagacatat
     3121 tattaaaaat attttgcttc ttaaaactgt gagataaatt ttctatctta atagaactac
     3181 tcatatatta atcctccttg aaattaatta taatatcaac caattcttct ctatttattt
     3241 ctaatttgtt agcttcttga agtaggtttt ttacaaaatt atgtttaaaa ttttcttttc
     3301 tagcttcttt tataatagtt tttgcaccat ctgatacaaa catccctatc cccctcttct
     3361 tatataaaat atttttatca accaatatat ttataccttt taatactgta gctggattaa
     3421 ttttataaag ctttgaaagc tctgttgttg atggaacctg ttcttcttct tttattccat
     3481 ttgatagtat ctcatcttca attgctcgtg ctatctgaat aaatataggt tcttcattat
     3541 ttaatactaa tttcataggt tcctcctttc tccatcagtt agttactcaa ctaattaact
     3601 atataactta taatatcttg aaaaatattt tctgtcaata ctaaataaaa aacagaggga
     3661 aaattccctc tgttttttgt tctttatatt tataattaaa ttttgatttt tgtaatccta
     3721 tcccataagt taaaaacttt aataatctgt tgtatcttca ttttttaata ttttacatat
     3781 tgagctagtt cctaaccttt ctgctcctgc ctctataaat ttttctgcat cagaaattga
     3841 tttaactccc cctgcagctt ttatttttac attttttcca acatgctctt tcataagttt
     3901 tatatcttct aaagtagctc cacctgttcc cattccagta gatgttttta taaaatctga
     3961 acctgacatt gttactattt cacacatttt tattttttca tcttcatcta aataacatgt
     4021 ctctataatg acttttaata ctttatctcc tatagctttt tttatttctt ttatttcttt
     4081 tcctatatta tcataatctt tattttttat atctgaaatg tttacaacca tatcaacttc
     4141 atctgctcca ttttcaatag catcttttgc ttcaaatact tttgtagcag tagtttgata
     4201 tcccaaaggg aaccctatca ctgtacatat ctttatttta ccttttagat attctgatgc
     4261 tcttttcaca tatgatggtg gtatgcaaac tgatgctaca ctcatatcta cagcttcatc
     4321 acataaaatt ttaatatctt cccatgtagt tgtagctttt aaaatagtat ggtctactgt
     4381 ttttaatata tgtttcataa aatcctccta aatttataat taatataatt agattacttt
     4441 tttaaatgca attttctctt tgcaatgttt agtatcatat caacctcttc aactttaaaa
     4501 ataatcatta gtagtgtata aattccacct ccaactataa ctgaaatagc tagtgataca
     4561 aactcattaa atgttcctac tcccaaaatt ccaaatataa atttatacgt aaagtatgat
     4621 aaaacaccca taattaaaga tgcaactacc gatttcaaag ttgcttttat tattttatct
     4681 tgtccaaaat atccaacttt tctctttagg ttcaagaaga gtaacaatat acatgcaatt
     4741 gatgatgaac tagttgcaag agcaagacca ccatgagcca ttggttttat taaaactaag
     4801 tccaatacta tatttacccc tacagatata atcccattta ccataggtgt ttttgtgtct
     4861 tgtaatgaat aaaaaacctt tcccaaaata tctcttaagc caaatgcagt cataccaact
     4921 gcataaaaaa ttaaggcagt tgctgtcatt tgggttgccc tcgcatcaaa agcacctctc
     4981 tcaaaaatga ttctaacaac aggagttgca aaaaaaatag aagcaactga aataggaatc
     5041 atagaaatta ttatcatatt tatactggat ttaactgaac tagtaaactt cttttgatta
     5101 ttttctgcac ttaacttaga taacatagga tacataacag atactattga agctgtaaat
     5161 gtccctgtaa caaaaccatt taatctatct gcataagtaa gtgctggaat gcttccttta
     5221 actaatgttg aggctaaagt tgtatctacc aatgagttca cttgattaac agcaacacct
     5281 ataaatactg gtgaaagtaa tgctagtaat ttaataaggc tatcatcttt aaagtttaag
     5341 tagtataagt atttgtagtt cgttttttta acaaaaaaca tgtaaaagag cagttgaact
     5401 accatagcaa ccacagcacc aattggaagt atgtatggtc caaatatggt acttaacatt
     5461 attgaaataa ttattactat attatagggt atacttccaa atccaactac aataaagttc
     5521 tctttaattt gtaaaaatgc tgacataaca ctcgtaatac ctatgaatat tattcctgtt
     5581 ctgaacctcc catgactaaa gtcacagggt tcctgcttca tagaattttg catactaaaa
     5641 tatgtcttac taattctcca caggctatcc ccgtagttcc tacggttctt acatacgtta
     5701 tttattttga attgttatta gtcttaatcc ttcttttaat atatttatac ttgcatttat
     5761 atctctatcg tgagtttcat tacaactagg acaaatccat tctcttatat ttaagttctt
     5821 aacttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa agaatttacc
     5881 tacctttaca atttgtctac cataccaatt agccttgtat tctaattgac gaataaactc
     5941 tgaccatgat acatcagaaa ttaagcgaga tagtttacga tttctaatca tattctttac
     6001 ttgtaaatct tctatgcaaa taatatcgtt ctcttttatt aatttagtag atagtttttg
//
LOCUS       DAMJYX010000154         6040 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJYX010000154 DAMJYX010000000
VERSION     DAMJYX010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.159"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            69..416
                     /locus_tag="PC479_003297"
     CDS             69..416
                     /locus_tag="PC479_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2805815.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(566..1066)
                     /locus_tag="PC479_003298"
                     /pseudo
     CDS             complement(566..1066)
                     /locus_tag="PC479_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1093..2598)
                     /locus_tag="PC479_003299"
     CDS             complement(1093..2598)
                     /locus_tag="PC479_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2805816.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2797..3651)
                     /locus_tag="PC479_003300"
     CDS             complement(2797..3651)
                     /locus_tag="PC479_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2805817.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
     gene            4036..4317
                     /locus_tag="PC479_003301"
     CDS             4036..4317
                     /locus_tag="PC479_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805818.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            4467..5315
                     /locus_tag="PC479_003302"
     CDS             4467..5315
                     /locus_tag="PC479_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDF2805819.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            5367..5888
                     /locus_tag="PC479_003303"
     CDS             5367..5888
                     /locus_tag="PC479_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805820.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
BASE COUNT         2142 a          844 c          800 g         2254 t
ORIGIN      
        1 tgagaatact attttcttat attggtataa taatagtaaa tctaatatag aaaagaggta
       61 tttctagtat gcatgaaaat aaaaaaaatc tattagacgg aagcttagct caatataatg
      121 atacagcagt tccaaaaata ccagtatttg ctggtaacga tataacttca gaagtttatt
      181 attttaaacc taatcaagtt cttaatgctc atagacaccc taatggtgaa caaatatttg
      241 tgttcctaaa aggagaaggt aaaatgaaac ttggtgagca tgaatgtgat gtaaaaaacg
      301 gcgatactgt atttgtacca acaggagagt ggcatgaaat tactaatgga agcaatgaag
      361 aaatggtagc cgttcaaatt acaaaaatta atgctggagc agaatacaga ggatagatat
      421 ataagtacag ttagtacttt aatatctatt ttattatata aactttaatc tcaaatataa
      481 aaagagtctt ttaaaaggct ctttttatat ttttgttaat tttcattata aaaacaagaa
      541 aatatctagc taaaactaat atattttatt taatattttt tatagcattg tttgcaaact
      601 cagtatttac gatatcttta taaggtgctt ccttatctag ttctttagct tctttcataa
      661 cagtcatcaa tttcttcaaa ccttcctctt ttaatacagg attttgagac catgcatcta
      721 ttgatttata tctatctaca actgttgtta aatcttctaa agatatatct tcaaagaaag
      781 gttgcatagc cttagctact tctttgctag atgcttgttg aatccataat tgacctttat
      841 aaacagcatt tgtaaatctt tgaattaaat ctttattgtt tttcatgtat gatctcgtag
      901 tagagtatgc tgtaaaaggt atttctccag aatcttttcc tatagatgta actatatatc
      961 cactaccttc tttttccatt gctgttgctg taggttcaaa tgctgttgta aagtcaccct
     1021 ctccacctgc aaaagctcca gccattacac caaattggta atcagtctat ttataacatt
     1081 taaaacatat aattaatctc ttgggatatt agggtaaagt attaaagaaa aatcatcaga
     1141 tttttgatta ggttcttttt tatagactgc gtaatttatt acacttttaa gcaatatatt
     1201 ttttagataa ggttcttttt cctctgtata ttcacttaaa attttttcaa gttgaggaac
     1261 aatttgtgtt ttaactgtat ttctttcctt ttgcatttca atatctatat ttgttttatt
     1321 aatagcttct cttgcatttt ctatacgaga taagagtatt tttaaacgtt ccccaaatgt
     1381 attactgtca taaattcctt gttctaaaaa atcatgtagt cgttcttttt gtttttctaa
     1441 agtatccaat tctttattta aatttatcaa ggtatgttct aaagtatttt caggtttaga
     1501 gttgtctttt tcatctttta aagatagtct ataatttact aaccatttat ttaagacctc
     1561 taatatttta ttttctaata tgtatagttt agtagcttta tttttacaat tagtacaaac
     1621 catatatttc tcatatccat ttgatgtagt attagatttg gctattaaag tatgattaca
     1681 gtatccacat tttacaattc cagccaatgg atttgttatt ttattgtttg gagtagggga
     1741 attaatacgt ttttttctta tattttgtgc cttattccat atgtcttcac ttattattgg
     1801 ttcatgcttt ccttttacct caatttgttc ctcaattcct cttgttctac tagaatttct
     1861 tttcctctcg actttattcc atactatata tccacaatac actttgtttt ttataatatc
     1921 tcttataccc ttttcatacc aagttctacc tgttgtggtc ttataaccaa gttcgtttaa
     1981 ataattggat attttacatg caccatctcc ttttaaatac atatcaaaga ttattttaac
     2041 aacagttgct ttatcttcat ttattacaag tactctttct ttattattta aatatttaat
     2101 atcataccca taaggaggag cttgagctat aaatttaccc tcttcaatac ttttgatttt
     2161 tcctctttgc atacgtctag atattaattt taattcctta cgagccataa aagcttcaaa
     2221 ttcagaatac tcctcatcaa actcatcatt taaatcatac gtttttctag gagtaataat
     2281 tttcgtttta gattttttaa atgtatctag tattaaccct tggtcttgca tattaccacg
     2341 acccaaacgg tcaatatcca tcactaaaac agcatcatag ttattatttt taatttcacc
     2401 tagtaattct agcatcttag gtctatattc tatactttcc ccagaaacaa gttcttcttt
     2461 aatttcaatg acatttaaat tttgttctct tgctagtttt aaaagagttg tcttatgtct
     2521 acttagagtt tcaaattcac ctctatgttc tgcttcttca tcagctctgc ttttgcgtaa
     2581 ataaattgcc acattcatta aatcacaccc cttgaatata ttatattcag taacagattg
     2641 attatattta aaacacaaaa ataaaccttc aaaatacaag atatattttt taggtttatt
     2701 tttatacagt aaattttata gtatatttaa aagcttgtaa tctaagtaaa tatttatact
     2761 attaatttta tgctttcttc attttttaat tttaaactat ttttttacta caggtatcca
     2821 tacttcaaat ttagcatttt gagggtctgg atttaagtac agttcaatat caggaccatt
     2881 atcatattca taaccagaag taggtaacca ttctgttaca atacgctttt ctagttcttg
     2941 tattgcttgt ggacattgtc cttctccaga aaatattgcc caagtaaaag ctggaacagt
     3001 gtattcttct aatgtattat ctatagattt tgtacttgaa acagatataa aatacttcca
     3061 ttcttctgaa ttattacaaa tacttatacc taatacacct tgaagttgac tatccatcat
     3121 agggattaat ttttgaagcg ttccatctaa agcaaccttt tgccacattt ggggaacaat
     3181 ttcaaaattc ttttctagtt cattatgaag tggctgagat acaccaacta tacgaaatgc
     3241 ttctttcttt tcaattctat aattcatttc ttcggctcct ttaatactaa ttttaaaact
     3301 gattggagga aaggctttta aagttgttat ttctcttttt tttataactg atggggcaac
     3361 accatgaata cttttaaatg cacgattgaa tgctgttgga gaagagtatc catattttaa
     3421 agccacatca attatctttt tatcatcatt ttgtaattct acagctgcta aagacatccg
     3481 tctacgacga atatattcag aaagtggtac atctgccata taagcaaaca ttctttgaaa
     3541 atgatatgta gaacaacaag ctatttttgc cacttgttca tactcaattt cttttgttat
     3601 gttttcttca atatagttaa ttgctttgtt taatctttca atccattcca taaaatcagc
     3661 tccttgtaat ttatataata tctattttaa aatagttaat cctctctttc tatgcactaa
     3721 ttaactagct taataaagaa tgaacatgag aaagtctgta attaactaca aaaatgaaca
     3781 aatagtattg tttttcacat taaagtatat cacaaattag tatctaattt ttatataaga
     3841 taacagtttt aatttattat gaaattttta taatatttta gcttagacat ttaattatat
     3901 ataacatttt atttatagtt aactattttt gaagtttacc cttaatcaag caacacttag
     3961 ttattcaatt ataaataatg attgagaata ggacaatttt taagatacat aaaatataaa
     4021 gaggagggga tttaaatgcc aaacatagaa aaagacttag atggtacaac tactataaca
     4081 tacctcaacg acgatatagg tgttccaaca gacgagaatg gatataaaat atctatgaca
     4141 atactacatg gaaagacaat ctgccatgta gtatctcctg aaacaatctt aggaagaaag
     4201 ttcacattag aagaaaaaca agaaaaagat acaataatat atggagcaat agaaaaaata
     4261 atggtagaca gacaattaca aataaaaaat tcaaacaaag aagttgctag tcattagcac
     4321 ttctttaata agtaaagtag gacaagtatt ttttataata ttattgactt atagtctaat
     4381 tagactataa tataaacata gatagtctaa ttagactact attaaaaata aatacaataa
     4441 gaatttaaga aaggataata ataataatga ttaaatcatt tttaatgtta ggacagtcca
     4501 atatggctgg aagaggattc ataagtgaag tgactccaat ttataatgaa agaatacaga
     4561 tgttacgtaa tggaagatgg caaatgatga cagaacctat taattatgac agaccagttt
     4621 ctggggtaag tcttgctgca tcatttgcag atgcatggtg ctgtgaaaat caagaggata
     4681 gaattgggct aattccttgt gctgagggag gcagttcctt agatgaatgg aatatagatg
     4741 gtatactttt taaacatgct ataagtgaag ctaaatttgc catacaaagc agtgaattga
     4801 caggaatttt atggcatcag ggagaaaatg acagtaataa tggcaactat aaattttatt
     4861 ataaaaaatt actttctatt attgaaactc ttagaaaaga attaaatatt ccagatatac
     4921 caattattat tggtggattg ggagacttct taggaaaagt aggttttgga aaaagttgta
     4981 ctgagtatgt atttatcaat caagagttac agaaatttgc ttttgaacaa gataattgtt
     5041 actttgttac agcaacaggt cttacatcta atcctgatgg tattcatatt gatgcaatat
     5101 ctcaaagaaa atttggatta cgttattttg aagcttttca taagaaaaag catattatgg
     5161 aagcattagc taatgaaagt gagttgataa taccgagtaa ttccaagaca tatacaaaaa
     5221 cagaaaaaat atatataaat agtatggact tggcactagg aaagatatca tatgatgagt
     5281 ttgaatctaa acttattaaa cttaatgata attagataaa atattcatta atatactaat
     5341 aaatatagat tgaaagaggt gattttttga taccattatt aattttagga ttattaaaac
     5401 aaaatccaaa ttcttatgga tatgagttat tagcattaat ggaaaagaag cattacaagt
     5461 acatagtaaa tttcactaaa ggttcttttt attataattt acagcaactt gaagaaaaaa
     5521 agtttattga aaaagttgaa aataaacaat ctaataaaaa tagagaaact cataactaca
     5581 taattactga acttggatgc caggaatttg aaaaattaat gtataagtat ggttctaaaa
     5641 cagagtatat aaatctatca ttttacgcaa cactattatt tgcaaatgat tataaaaatg
     5701 aaaagttaat agaattattg gaaacgcaaa ttaagcaaac tcaagtaaga atttctctat
     5761 taaatgaatc catagaaaat gacaaaacaa attcacatta ttttaataga atgctagaaa
     5821 attctcgttc tcaccattta gttaatattg aatggtttga gaagttgcta aatgatatta
     5881 gagattgatt ttaataataa aaaaacatac atatatattt tatataaatg tatgatatat
     5941 agattaaaaa taaggcagat aattttcaat tgaatatctg tctttttgtt atataagatg
     6001 ttaaaagaaa cttatactat gtaagtaaaa tttataaata
//
LOCUS       DAMJYX010000155         6029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJYX010000155 DAMJYX010000000
VERSION     DAMJYX010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.160"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            125..937
                     /gene="modA"
                     /locus_tag="PC479_003304"
     CDS             125..937
                     /gene="modA"
                     /locus_tag="PC479_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2805821.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            947..1738
                     /locus_tag="PC479_003305"
     CDS             947..1738
                     /locus_tag="PC479_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805822.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1740..2435
                     /locus_tag="PC479_003306"
     CDS             1740..2435
                     /locus_tag="PC479_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2805823.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2422..3153
                     /locus_tag="PC479_003307"
     CDS             2422..3153
                     /locus_tag="PC479_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805824.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3467..4267
                     /locus_tag="PC479_003308"
     CDS             3467..4267
                     /locus_tag="PC479_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2805825.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4807..5523
                     /locus_tag="PC479_003309"
     CDS             4807..5523
                     /locus_tag="PC479_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805826.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2350 a          726 c          846 g         2107 t
ORIGIN      
        1 ttcttaacta tacataacaa aacataacaa aacattataa aacataacaa aaacttagac
       61 atcgttttct tagtatgata tactaaaaaa gaatttacat taatttacta agggggaata
      121 tcttatgttt aaaaaacttt cagtactagc tttagctatg attatttcat taagtattac
      181 tgcatgtaat tctaaagaaa acaaagctaa tgatgaatct aatactaaag tgacaacagc
      241 tgatgattca aaagaaaaag agactctttt tgtctattca ggggcaggtt taaagaaacc
      301 aatggatgaa atagcaaaaa aatttgagaa agaaaataat gtcaaagttg aatatagcta
      361 tgcaggttct gcacagctaa tagctcaaat tgagacaagt cacaaagggg acgtatttat
      421 tgttggttct gaaccaattt attcaaaagc ctttgaaaaa gaacttgtag gtgaacataa
      481 aacggttgca catcatacac ctgcaatagt tgtacctaaa ggaaatccag ctaatattca
      541 aaaattggaa gacttaaata aagatggagt taaacttatt ttgggagaca aagagtctaa
      601 tgctataggt aagactacac aaaaaatact tgaaaaaaat aatttaaaat ctataaatga
      661 taatgttgtc tctacagctg caactgtaaa tgaaatgatt gtacagttaa catcaagtaa
      721 agctgatgct acaatagcca caaaagattc ggtctttggc aatgaagatg tagaagttat
      781 agaaattgcc caagataaaa atatagacca acttatatca gcaggaatcg taaactatag
      841 tgataaaaaa gatttagcta ataaatttat aaattatata gagtcagacg aagctaaaca
      901 gatatttaaa aaatatggtt ttgaacctgt taagtagtga ttaaacttga aaaataaatt
      961 ttttactact ctacttactg gattttttgc tatagtcttt ttgtttattg tatcaccact
     1021 tcttatgtta atcattaaag ggataagtta tgttcctata tgcttaaaat cagtggaagt
     1081 acagtatgca ataatactta gtattaaaac atctttaata tctactatag tatgcctttt
     1141 attagctatt ccagtagcat attttttaca tattactaaa ttaccattta aaaaactgat
     1201 tatacagata ataaatctcc ctatgtctct tccacactta gtatcaggaa ttgctctttt
     1261 acttttgttt ggaagaatgg ggattggaga ttccatttat aagattttta agcttgattt
     1321 tatatttaca aagcaaggta tagttttagc acaagtattt gtaaatcttc cacttactat
     1381 taaaatactt tacacttcac taaatgaaag taatgaaaag atgatatttg ttgcacgtac
     1441 ccttggatgt aatagttggg aagcatttag atttataatt cttccaaatc taaaaactgg
     1501 aatcatatct gcaactgtaa tgacttggtc aagagcactt ggtgaatttg gtgcagtagc
     1561 tatgatagca ggttctacac gtatgaaaac tgaaattatt cctacatcta tatatttaaa
     1621 tatgtctact ggtgatatag acattgcaat cggaatagct gtaattttga tatttatctc
     1681 acttacatgc ttgatgcttt ttgagatatt ttttaataga gaggttgata agaattaata
     1741 tgttaaaaat aaaaaattta tctcttgatt tacctaattt taaattacaa gaaaatagtt
     1801 taaatattaa taatcatgaa tactttgttt tattaggagc tagtgggtca ggtaaaactc
     1861 tatttctaga aacattagca ggaagatatc catctgctaa aggaaatgta tcatacaaag
     1921 aaaatttaat atcaaactta cctccagaaa atagaaatat aggatttgta tatcaaaatt
     1981 ttgaattatt tccaaatatg aatgttgaag ataatataag gtttccactt aaattgagaa
     2041 aaatacctat agatgaacag attcttaagg ctaaccactt atctaaatta atgcagatag
     2101 aagatataaa aaataggtat cctaaaaatt taagtggagg tgaaaagcaa cgagttgctt
     2161 ttgcacgtgc cttaatagct gaacctgata tattactttt agatgaacct atgagttcat
     2221 tggattatat tacaaaaaaa catataagca aaattttgaa ggatgtctac ataaaataca
     2281 aaccaacagt gattcatgtt actcatgata tttctgaagc aatgtttttt gctcataaga
     2341 taggaataat gaacaatgga aaaataaatc atatttttga tttaaatgaa gaaataaaaa
     2401 ataaaggaga atcttttttc tatgaatata tttgatattt tgagagatta tctattagta
     2461 caagttgata aatataattt aaatatggat atgataagta ttgtgtccaa atctctatct
     2521 tcaaaagaag caattggaaa tactaagcgt aaagatttcc caattatagt tggtaaagaa
     2581 ataatgcttg aagctgactt taaaggagct aaaggacaag catttacttc tactccatct
     2641 acatttgaag gttctttaaa agacatatta tcattagatt tacatgacaa tcctcatgat
     2701 agaagtcttt ttatagcatc attaaatgca gttatgaaat acttaggcaa gacggataga
     2761 accattcatt gtaaaaataa cgagccagag atatgtgcaa aaaaattccc tgagtttata
     2821 aaaatggaat ttggtaatcc aaaagtagca attataggat atcaaccagc cataattgat
     2881 aatattaagg actttttcga aacaagagta ttagatttaa atcctgaatt tgtggatact
     2941 atccaatata atgttaagat tgaagatggt atacgagatt atgaagatgt aatatcttgg
     3001 gctgatttag tgatttgcac aggaagtaca ttatgtaata actcaattat aaattttcta
     3061 tcacttaaca aaccagttta ctattatgga actacgatag ctggtgcatc aaatatttta
     3121 ggtttaaaaa gactctgttt ttgtagtaaa taagcaatca tatcatcaag gagtacacaa
     3181 atatatatgt gtactcttta aatttaaaaa gttattactt ttattctaag tactaaagta
     3241 aaattctatt aaaatgaatc ccgaataaat gtacaatccc tttaaatctc aattcctatc
     3301 aatcattaat attttgatta gtcttataca taaatgtttt ctttatttta aactaataaa
     3361 aaaattttat tccattaatc tactctatat ttttcagcat ttaagaaata atttttagta
     3421 ctttgtttga taatataact aagacaactt ttcaggagat gattaaatga catttaagaa
     3481 taaaaaattt actataaata aatataaaat tctatctatt attctagttt tatgtttgat
     3541 tttaaccgct tttagtagct atgtgaatta tagtcataaa aaatttaaag attatgttgt
     3601 cttctatgtt cagcatcaag atgatgaagt gttgtgggct gggagtgcta ttgttaatgc
     3661 tataaaagaa agaggaaaaa atcatgtatt tgtggtttta gtatctactg gatgtggtat
     3721 atccgtcttt gataaatata aaaaatatga aaacctaaca aatatacaaa aatcagaata
     3781 tagaaataga gaatttttag cttctcttaa tagccttggt gtaataaggg aaaatataat
     3841 attattacca gaaacaaata caaatggtaa tactgacttc aattacatgg aaaaaattgc
     3901 tcttacattt gaagaaaaat ttaaaaatgt aactcatata gcacatactt ataagttaga
     3961 tgaccattta caacatttaa aaaatggttc tgttatccaa aatttatata atgctggtaa
     4021 aataaaagat gctaaatatt ttgtaaaacc taaatttgct gataaaataa cttttaataa
     4081 taagatagtt tataaagctc attctactga agactatgaa aaggttaaaa atgcctgtca
     4141 acaatacaaa attgttgatg aattagaata tagagaaggt attggctata agtctgacca
     4201 taaatcattt gataagttat taaataataa agatgttcct gctatacttc atacacctaa
     4261 tttataagaa atatttattc ttttaattta agttataaaa aggaaattat ttgagatttt
     4321 gtgatatagc actttttgtg ctatactact ttttctcata caactattac ccatatctaa
     4381 tttcatcatc tcttaaaata tataaagata tcatcattaa aacctataag ccttattaaa
     4441 tgctttatac atatctacat taagctacct atttatatta aactacatta aaataaatat
     4501 ttgttataaa ttcttaataa ttatgaaact catttgttat ttatatttgt taatatatgt
     4561 ttaaacctaa aaaattaata aaccaaaata acataaggag tgtgatagat taccctagat
     4621 attttcataa acttttacct tcagattaat atgggcgtct atacttttaa atttactata
     4681 tgcattgaca atatattaaa agtactagtt tttatttcat aatgaacggg cttactgttt
     4741 tctaatacct ctttttggta taaaataaaa attggttaaa aataaaattt caagggggtt
     4801 aataagttga atcaaaatat attggttgta gatgatgata gagagatagt tgatgctata
     4861 gagttctatt tgagacctga taactttaat gttttaaagg catatgatgg acttgaagca
     4921 ttggacatac taatcgaaaa tgatattaag ttaataataa tggatgtaat gatgcccaat
     4981 ttagatgggc ttaaaactac acttaaaata agagaaaaaa ggaatatacc aataattctc
     5041 ttatctgcaa aatcagaaga tatggacaaa attataggtc ttaatatggg tgctgatgac
     5101 tatataacta aaccatttaa tccactagaa cttactgctc gagtaaaatc tcaacttaga
     5161 aggtatataa atttaggtga ttttagtaat acaaataact caaatgatac taattatatt
     5221 ctaaaaagtg gtggcctaga gctaaataca gatacaaaaa tagtttcttt agatggtgaa
     5281 gaaattaaaa tgacaccact tgaatataaa atacttacac ttcttatgtc acgaaaaggt
     5341 aaaattttct cttctcatga gatttatgaa aatgtctgga atgaggatgc ttatagttgt
     5401 gagagaacta ttgctgttca tattagaaga attagagaaa agattgaaat taatccaaaa
     5461 gaaccaaaat atttaaaggt ggtgtggggc attggttaca aaattgaaaa acttaattat
     5521 taatgattct aaagcaaaat ttattttatt attattactg tttctattag tttctatatt
     5581 atttgaagca gtttatgatt tgaataataa cttttcttat aatattaatt attattataa
     5641 agagtttgct ctcccttatt tttatttttt cggtactcct gtacatttgt caaaagtaga
     5701 attagagttt gtctgcatag gtattatatt atcaagtttt ttattgacta tactatgttt
     5761 tacattgtat aaaaaaaata aacagactaa agtcaaattc ttagagaatg tcataagcct
     5821 tttttctaag gtaaaaatag aacttacact tatattaact ttgatactat taaccttaag
     5881 gtttcatgca tttacaagta ataaaatgtt ttacaacact aatttaataa tctacaattt
     5941 tataattata tctatagtct atataatata tgaaaatatt aaatataata actataatct
     6001 aaaagatatg tctataacat atgtatttt
//
LOCUS       DAMJYX010000156         6016 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJYX010000156 DAMJYX010000000
VERSION     DAMJYX010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.161"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(143..2737)
                     /gene="clpB"
                     /locus_tag="PC479_003310"
     CDS             complement(143..2737)
                     /gene="clpB"
                     /locus_tag="PC479_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2805827.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(2774..3370)
                     /locus_tag="PC479_003311"
     CDS             complement(2774..3370)
                     /locus_tag="PC479_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805828.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(3387..3791)
                     /locus_tag="PC479_003312"
     CDS             complement(3387..3791)
                     /locus_tag="PC479_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2805829.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            4158..4532
                     /locus_tag="PC479_003313"
     CDS             4158..4532
                     /locus_tag="PC479_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805830.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            4529..5392
                     /locus_tag="PC479_003314"
     CDS             4529..5392
                     /locus_tag="PC479_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805831.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            5392..>6016
                     /locus_tag="PC479_003315"
     CDS             5392..>6016
                     /locus_tag="PC479_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2805832.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYS"
BASE COUNT         1981 a          924 c          692 g         2419 t
ORIGIN      
        1 cttcctttga gttaaattta atttggacat attgacttaa aaatgtttta ttaatttgat
       61 aaaatatttc ttttcctata gtttataaat aaaaatcagc tgagcaaaat atttagtata
      121 ttatgctcaa ctgattcttt tattatttaa ctaaaacttc aatattttca tcttttccat
      181 ctataactat agtacatcca ttatacacat ctccagcaat aagtttcttt gcaattatag
      241 tttctaatgt atttcctata tatctcttaa gaggtcttgc tccatataca gggtcatatc
      301 cttctctcac cataacatct tttgctgaat ttgtaacttc aatagtaata tctttttctt
      361 ttaatctatt ttttaaatct ttcataaata tatctataat tttcttgata ttttcttggt
      421 ctaaaggctt aaacattata atatcatcta ctctgtttaa aaactcaggt ttaaatctat
      481 gcttcatttc attcattact aaattgttag tagtctctgt tatatttcct cctgcttcta
      541 atagataatt acttcctata ttagaagtca ttattataag agtattttta aagtctactg
      601 tcttaccttt attatcagta agtctaccat catctaatat ctgtaagaac atgttaaata
      661 catcctcatg tgctttttct atctcatcaa atagtattac agaataagga gcacgtctaa
      721 ctgcttctgt aagttgacca ccctcttcat atcccacgta ccctggagga ggtccaacta
      781 gtcttgatac agcatgtttt tccatgtatt cagacatatc tattcttatt atattatctt
      841 cactatcgaa taaatttcta gcaagagttt ttgcaagttc agttttacca acacctgtag
      901 ggcctaagaa aataaatgaa ccaattggtt ttctttcatc ttttaatcct gctcttgcac
      961 gtattactgc atttgaaact gctgtaactg cttcatcttg tccaataact cttttatgaa
     1021 gttcatcttc tagtttaagt aatttttctc tttctccttc aactagttta gttactggta
     1081 tacctgtcca ctttgataca atgctagata tttcttcttc agtaacttct tcttttaaaa
     1141 gagaattttc agaaccatca ttcatatttt cttcgtactg ctttatttgt tcttctaact
     1201 taggtatttc accatattta acctctgctg ctttgttgaa atcatattct ctttcatact
     1261 tttcagcttt acctttagct tcgtctaatt gagcctttaa gtttttaata tctaatattt
     1321 gactcttttc tttttcatat tttgctgtca tttcatcatt tttagatttt aactctgcta
     1381 tttctttttg aatatcatcc aatcttgatt tacttttatc atcattttct ttaagtaatg
     1441 cttctctttc agtttctaat gtaaataact ttcttctaac aacatctaat tctgttggca
     1501 atgaatctat ttcactacga atcatagctc cagcttcatc aattaaatca attgctttat
     1561 ctggtaaaaa tctatcttgt atatatctat gagaaagttt tgctgctgct acaatagcat
     1621 tatcatgtat tcttactcca tgatgtatct caaatctttc ttttaaacct ctaagtatag
     1681 atattgtatc ttcaacagta ggttcttctg caattacagg ctgaaaacgt ctttcaagag
     1741 ccttatcttt ttctatatac tttctatatt catcaaaagt agttgcacca atacagttca
     1801 attcacctct tgcaagcata ggtttaataa gattacctgc atccattgaa ccttctgttt
     1861 taccagctcc aacaatagta tgaatctcat ctataaacag tataattttt ccatcagaac
     1921 tttgaacttc ttttaaaact gcctttaatc tttcttcaaa ttcacctcta tattttgcac
     1981 cagcaattaa agctcccata tcaagtgaga atacaatctt atcttttaaa ccttctggga
     2041 catctcctct tactattctc tctgctaacc cttctacaat tgcagtttta cctacacctg
     2101 gctcaccaat caatacagga ttattttttg ttcttcttga taaaattctt ataatccttc
     2161 ttatttcttc atctctccca attacagggt ctaatttatt tttcttggct aaatctacta
     2221 aatttgttcc atatctagca agtgcatcat atgtgccttc tgggtcttgt gtttctactc
     2281 tttgactacc tctcacttgt gataaaatat ttaaaaagtt attcttattt atattatatt
     2341 gttttaatat tttaccaaca gctgattttg attctgtttc catcattgca agcataacat
     2401 gctctacact tatatatgaa tctttaaatt ctttagaaat actctctgcc tttataagca
     2461 cctcatttat ttttctagta gctgatactc cttgtgagtc agcaccttct ccatatacct
     2521 ttggaagctt atcaatttca aagtttacag tatttctcaa agaatcaatt gatatattca
     2581 ttttttcgat tatatttggt atcaatccat cctcttgatt aataagtgct gagaaaagat
     2641 gaattacatc tacttgttgg ttatgatttt ttactgcttc gttataagcc tcatttaaac
     2701 ttttttgaac tcttaaagtc attttttcta cgtccatata aatccctcca ttttaatatt
     2761 atatccaaat aaattaaatt ctctcattta taattattga ttttctaagc tctaaaagac
     2821 tatcaagtaa attctcgttt ttttgtatat cgaaactcaa tgttaaatta aatgttccat
     2881 ctaaattaaa aaaatcgaat acttcttttg attttgctat taaatacaag tcttcatcta
     2941 taagtctttt ttcacctatt aataaatact gacatttatc tataaaactc tttttaaaat
     3001 caatagtgta tagattttct aaaatattat attgataaat agcaatatta ttaaacactt
     3061 ttaatatgtt tgaatttaca aaattgtaaa tttgattatt gttatacagt gatatttgtt
     3121 cgtatatata ttccctaaat aaaggatgaa tgttttgctt aagttttaaa gtaacttcag
     3181 ctggtatttc aaaataaagc ttccagtact tagagttatt tttttctaaa ataaaagcta
     3241 ctttgttcaa actggtagaa ctaatctcct ttagtgtaca attaaaatta tagtgtctca
     3301 aactagtttt ttcattctca aatacatagt caaaccactg tagaacattt ctatcaagtt
     3361 ttaaactcat ataaaaacct tcttccttat ttactaacct tattaggtct ttttatctga
     3421 ttatattctg ctaataaact ttctttatca gctctactac ctttatatgc tatatgtgga
     3481 tttatcatat actcaccatt tttcactctt tgtaaaaaat ccattttttt cagctccgta
     3541 aacaatgaag ataaagaagt ttttggatat tcaaattcac aagataattc atataatgtt
     3601 tttctaatct tattattttt atccatagag tttattaatt ttaatggaat taaatccctc
     3661 ttagtataca ttccttctac tactttaata taattcttaa aacaaacatt catatactta
     3721 cttttattca taattaaact tttgctctct gtatattcct gatttaaatc cttttttaat
     3781 gtcttatcca aattaaatat ctcttctcac tctccttatc tctaattgat aatataaata
     3841 tattacaaaa aaatatgtat gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa
     3901 cttttattta gctataagct aaactttatt atttttttaa tttagtattt ccaatttatt
     3961 ggcaggattt ttgaacaata tttataaatt ttttaaaaaa atttaaatct atttaatatg
     4021 gcagactttc ataaaataca ttacatttat taaatgtata atattggtaa ttagtttttt
     4081 ctaaaatcag ttgacatatt atatatatat ttatataatg tgtatatact atatatacaa
     4141 tttaaggagg tgtcttattg aatatagtta ttagtaattc gagtgggaag ccaatatatg
     4201 aacagattac tacacaaatt aaaagtatga ttataagtgg agagttacca gaaggctcag
     4261 ctcttccaag tatgagacta ttggcaaagg aacttagaat aagtgtaatt actacaaaac
     4321 gagcatatag cgatttagaa cgagacggat ttattgagac agttactggt aaaggcagtt
     4381 ttgtatctag taaaaacatg gattttgtca aagaagaaca atttcgttta attgaagaat
     4441 acctacaaaa agcagttgat acagcaaaaa gtagtgatat tacatatgac gaattaaaag
     4501 atattttatt tttattatat aaaggagaat gataagtatg gatgcaataa ctataaaaaa
     4561 tttaaataaa acttataaag attttagcct acaagatata tctttttcag taccaaaagg
     4621 gagtgtaatg ggatttgtag gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat
     4681 tttaaattta atatcttatg atagtggaaa tatagaaatc tttggtcttg atagtaaaaa
     4741 aaatgaaaaa gaaattaaag agcaaattgg ggttgtattt gagggaagca actttcatga
     4801 aaatttaaac actgaccatg tttcaaaaat catgagtaaa atctataaaa attggaatga
     4861 cacattattt aaagactact taaaaaaatt gagagtacca gacaataaat taattaagga
     4921 gttttcaaag gggaataaga tgaaacttag tattgctgta gccttatctc ataaaccaaa
     4981 attattaata ctagatgagg caactagttc acttgaccct atcgttcgtg aagaaatact
     5041 agatatattt ttagatttta tacaagatga agaacactct attatcttat cttcacatat
     5101 tacaagtgat ttagataaga tagcagacta tattacattt atacataaag gaaaaattgt
     5161 atttagtgaa aataaggatg aattaattga tactatggga gttttaaaat gtaaacctag
     5221 tgattttgat aacctttcaa gagaggatta ttcatattat cgtaaaagcc aatttggata
     5281 tgaagtactt cttaaagata agcatagatt tatctcaaga catccaaact gtattgttga
     5341 taatacgtct atagaagaaa ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt
     5401 ctaattttaa aagatttact taatttgaaa ggaaatataa agtttatact tttattcatc
     5461 attatgtttg gctttatgtc ttctttagga gatggaaatg ttaataactt tataggtgtt
     5521 attatagttt tatgtactac aatgattgtt tcaacatttt catatgatga tttgaataaa
     5581 tgggatagtt atgtacttac aatgcctatt aatcgcaatg atattgtatt aagtaaatat
     5641 ttaactatgt taatatttag ctttataggt gttcttgttt cacttattgt tagtgtaact
     5701 attggttatt ttaaaaatac acttatatta aatgaaacac tacttattaa tgcactaatc
     5761 ttatcaattt cagtatgttt tggtagcctt attctaccac ttatttataa atttggtaca
     5821 gaaagagctc gtttattaat gattttatgc tttttagttc caacactagc tttattagtt
     5881 tttaaaagta ttttagaaaa tattagttca cctatttcta tagaaattat acttaataca
     5941 ctagtatatt ctcttccatt tgttgctatt ttactatttg tgatttcata ttttatatca
     6001 tcaaaaatct atagta
//
LOCUS       DAMJYX010000157         6016 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJYX010000157 DAMJYX010000000
VERSION     DAMJYX010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.162"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            373..627
                     /locus_tag="PC479_003316"
     CDS             373..627
                     /locus_tag="PC479_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805833.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            663..986
                     /locus_tag="PC479_003317"
     CDS             663..986
                     /locus_tag="PC479_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805834.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1260..1412
                     /locus_tag="PC479_003318"
     CDS             1260..1412
                     /locus_tag="PC479_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805835.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1606..2529
                     /locus_tag="PC479_003319"
     CDS             1606..2529
                     /locus_tag="PC479_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2805836.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVRA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   2796..3611
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2796..2824
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            3802..3954
                     /locus_tag="PC479_003320"
     CDS             3802..3954
                     /locus_tag="PC479_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805837.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4074..4887
                     /locus_tag="PC479_003321"
                     /pseudo
     CDS             4074..4887
                     /locus_tag="PC479_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            4939..5100
                     /locus_tag="PC479_003322"
     CDS             4939..5100
                     /locus_tag="PC479_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805838.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5428..5892)
                     /locus_tag="PC479_003323"
     CDS             complement(5428..5892)
                     /locus_tag="PC479_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805839.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
BASE COUNT         2452 a          568 c         1029 g         1967 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaaa cctgtgactt tagtcatggg aggttcaggc
       61 tagtattact cattattgta ttcaaaagct aaagtggaaa cctagtgaat atatgaattt
      121 agaagttaat gagagagcat tagcagccga ctcaatactt attaaaattg aagatgaaga
      181 agaaacaatg aaagaagctg aaagagagaa aaagagaggt agaagaaggt agaatttaaa
      241 gttaaaatag ttacattgct aatatatgaa aatgttacaa atttggaaat agatggatat
      301 atatgttgta tagtataatg tagataatat atcaccaagt aaaaaacaac aaataaatct
      361 aaattatgag gggtggaaaa taaagatatt aaacttacaa tatgtatatt tgcttttgta
      421 attggaataa tagtgttatt ttgctgtata ggtctaggtc aatttaatat ggattgtatt
      481 ataaaacaaa atggtgggtc aatagctacc gaacaataca acatatattt agaacaatct
      541 atttcacaat atagaaattt tggttctata ctggttttac taggaggaat aggaatactg
      601 tttgataaaa gtgtcagaaa atactaaaaa agtatatttt tatttaggat gtgatgaatg
      661 atatggcaag agatgtattt agtgtaaaga tgacaccact agaagcacag aaaatggtag
      721 aaaaaaattt ggaagcagat ttagtttctg ttgatattta taacctagaa aataataagt
      781 ctataatagt tacaatcttt gaaaaatatt atgcaagaag caatagtgat gcaggtttgg
      841 tagtgatttg tgaaaatact acaggaaaaa cacttgttaa aattacctca actggtagtg
      901 caattggact atttcagatt gattggggaa caggtaataa tttgattaaa agagtaaaaa
      961 aaatacttat tgattatatt atttaattat tttatgtaga tattcttagg ttggtatgga
     1021 atttgtacta acctattaag taatcaataa aactaaataa acatagaaag cacttacaaa
     1081 tatgtaggtg ctttttattg aaaaaataga aaataaaata aaaagtaaga tataggaaaa
     1141 atatgtaaga attatatgtt ataataattg tagcaagaag atgtaatcta caatttatag
     1201 agtggagttc atgtattaaa aaattatcct cccaacgata agaagggagg tgaatataca
     1261 tggataattt tttatttaat gtgttagcta gtgtaacagc tagttacata gtttatttga
     1321 ttagcaagtt aatcaaaaaa gtaaaaagcc actctaccgc aaagagtgac ttaaagattg
     1381 atataaattt taaatatcat cgtaaaaaat aactttcagt tatagaactt cactctaccg
     1441 caaatagatt gtagttcttc ttgtttttat tataccacaa attagaaaaa atattgcata
     1501 taatattttt tatagtcaat aaaaatatga aattttttat acacaatgat gatgaatcta
     1561 actagatgaa taaatttaat tgtaaataag aaagtactta cttaaatggt aggtgctttt
     1621 gttttgccaa attatgctat aatattgagt gagatgagta atttgtgggg gaattttata
     1681 atgtggatat tagtaccaat aataacaata gtattactta taatagctgt ttcttctatg
     1741 caatatattt tagttatgat agcttttttg ttgattatat attcttttat agaaaagaaa
     1801 atagttatgg gtcttgtgtc agttcttttt ttcacttatt caatttatct ttgtgcaact
     1861 tgggaagata aatccttgat agcagataat aaagttgaga ctgttagggc acaaagagaa
     1921 actgtagaaa gagaaaaaga aatggaaaga agaagaatac aagaagaagt agacaaagaa
     1981 agatatattg aaaaacatgg aatggaaata tcagaaaatg atttaaaagt taagctagag
     2041 gctttagtac ctcaagaata taaggggaag aaatatgaat taaaagtagg aaaatttaaa
     2101 agatatagta tgtattttga tttaactgta caaaatgaaa aatttagtaa ttctgaagaa
     2161 tgtaaaaaat ttgtaaaaga aattgctaat gatcttaaaa aaattaaaat aagcaaggcg
     2221 tactttaagt ttcattcaaa agatgacggc ggtatatata attctgttta tattgattat
     2281 tttagaaaca tacaaaataa tgtagataat gttgaaaatt tagaattcaa tgagtttgaa
     2341 cttaaaaccg aagaagaaga gaagcgagaa caagagaaaa tagaacaaga aaaaaacagt
     2401 tataataact acattcaaaa cagagtggtt gacccactag atagaataaa gaaactaaaa
     2461 gagttgctag attcaggagc aattacacag gaagaatata ataaaaagaa aaaagaatta
     2521 ttagaataga taatatagta agcacttaca agtatgtagg tgcttttaaa tttacaaagt
     2581 attccatttt aattttatag tttagatttt atgatataat aaaaatatag aagttttgca
     2641 gtgtgcgata tttgttacaa agtagggctt aatacttgaa atctaagatg ttgagggtgc
     2701 gtgataagtg ttatcaattg cactattgcc cgctcactgc aattttaaga gtattgtata
     2761 tatgtaggta ttggaaatgc taagtttatt ttggggtttt agattaacta tatggaatgt
     2821 aaatattaca tgggtaaaaa ttttgatgat ttttatgaca gttttagatt aactatatgg
     2881 aatgtaaatt gtatagaatt aagaattttt ttaatactaa ttatagtttt agattaacta
     2941 tatggaatgt aaatattaca tttaatttgc attgctttga tgacttcttt gttttagatt
     3001 aactatatgg aatgtaaatg cgtacaaggt tgagaagctc cgcaatctca acgtcgtgtt
     3061 ttagattaac tatatggaat gtaaatattt gaaaggaata aattttaaat gaatgaaagg
     3121 ataggtttta gattaactat atggaatgta aatcagccaa aaacttcgaa tttgaactaa
     3181 aatgcgtggg ttttagatta actatatgga atgtaaattc tcatgactat tgaataaata
     3241 taataaacaa gcatacgttt tagattaact atatggaatg taaatttaga atcaaaagca
     3301 cttaatccta gttctttcat agttttagat taactatatg gaatgtaaat agctttttag
     3361 cttcgtctac tactttataa gcaaagcgtt ttagattaac tatatggaat gtaaatgatg
     3421 aacaataaaa caatcatcta aagacgaaga aagttttaga ttaactatat ggaatgtaaa
     3481 tgttcctgtt gtgtttttta ctaatcccat tttatatgtt ttagattaac tatatggaat
     3541 gtaaattgct gtgatttcac taataaaaca attaataatt gtgttttaga ttaactatgt
     3601 ggattcaaaa ttaaataaac aaagaaagca cttacaaata tgtaggtgct tttattctgc
     3661 tcaaaattgg tcggttgggt aaaatcatta gaaaaaatta gtaaaaacct cttttctgta
     3721 actcgttaca atattattat taatgtaacg agttacagaa aagaggtgaa taaaatagca
     3781 actaaaagta gggcagagta tatgaaaaat tgtcgaaaag ataaaagagg ttttagtgta
     3841 cttttagaca aagaaaagtt agataaattt gatgaagtat tagaggaaaa gaatctaacc
     3901 aagaaagaat ggctagaaga aaaaatcgac gaggaactgg aacaaaagga ataaaaaata
     3961 agggtcactc ccaccgacca aagtttgagt aacccctatg atgtatacta tcgtatatca
     4021 attataatat atgtcattcc ttaaaaaaat caattattaa ggagtgtaat attatgaaaa
     4081 atgaattaat gatgtttgaa ggaaaagaga ttgaagtatt tgaatttgaa ggaagaattt
     4141 tattcaactc aaaagatgta gcaaattgtt tagacataaa aaatgttaat gagaatataa
     4201 tacttatgaa tgaaaagcaa gttgtaaagt taagaaattc ggatatcagt aatactgaca
     4261 tccgaaaact taataatgct ggagaaaatt ttcttactga aagtggagtg tataaattaa
     4321 tctttaaatc aagaaaagaa gaagctgaga gattccaaga ctggataagc gatgaagttt
     4381 taccaagcat aagacaaact ggtgcataca taactaataa tgtaatcccg aaaagttgag
     4441 agaaaaagca agtgagattg aaaaattaca gttgcttata acagtacatc tatgttaaaa
     4501 gaattgctag atgatgcagg ctttgacaat aaatcaaaac tattaacagc taaaacatta
     4561 tataagaaag cagaaattga tttaccaata gagataaacg aagaagaaca ttattttgat
     4621 acaaaacaaa tagcatctaa actgaaaata tattctaaga gtaataaacc agctcagatg
     4681 gctgtttgtg agattattaa aaagattgat ttagaagatg gtgaagttaa aggtgtttgg
     4741 gaaattaatg gttcttggac tggtactgta aataaatata caaagagtgt aatagataag
     4801 gtgagaactt ggatagagga aaataataga cctaccaaga ttgcaggtga gaagaagaat
     4861 tattatgtat tttacaaaat tgagtaaatt tatcagttgt attaaataat ttagtttagt
     4921 tttgggggga ttaatacaat gtgtgagaat ttacttgata atatgtatat tgaaaagaga
     4981 aaagaagaat atagaattaa acttttgaaa ataagagaaa cagatataga tatatataat
     5041 aagttagaaa gtatagtata taaactttct gagaaaaaat tagagaaaaa taataaatag
     5101 acatataaag cacttggata ttctactgtt tcaagtgctt gtttggtata aaatggtata
     5161 atggagatag agaattatat taaccaaaaa atggagaaaa tagagaagat gatttagttg
     5221 acgctttatc agtaggagta cctgcaggta agacagaatc tctagtacga ttagttggag
     5281 atggaataag tgatactcaa aaaagtttaa taaataaaag agaatttaga aatataactc
     5341 aaattggtgg aaatggaaat gagaaagcat ttagtgaagt agaaaactta gttaaataga
     5401 ttagaaggta gcaactagag ttggttgtta ccttcttttt ttatactttc tctctctaag
     5461 tattttattt ttcatatctt ctaatacatc taatgcagta gtataaaatt gtatattatt
     5521 tctataaggt atagctattg gaagaatgcc cttgttaact aaaaaaataa gaagtacaat
     5581 aaataaaagt gtcataatca ttctaataaa ataattagtg atatctgata aatcaaatgt
     5641 taagttaatt gttgtagaaa atacaacagc cataatagat atatacaaag caaaaatggg
     5701 gttactattt ttttgtaact gtcctttaag cctagattgc tctatttcta tatcttgaat
     5761 actttttatg gaatccattt cactataata cttataaact tttaaataat attttttata
     5821 cttgtttaaa gcttcttcat tatctttaag gtctttgtca cttaactctg atttaacttc
     5881 cccaaaatcc ataaaatccc tcctacaatg ttatataata tactttaatt tatagcatat
     5941 ttaagaactt atatcagcaa tttgtcgaac gattttagct aaaaatagag tataatgata
     6001 ggcttattat ttataa
//
LOCUS       DAMJYX010000158         5948 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJYX010000158 DAMJYX010000000
VERSION     DAMJYX010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.163"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            75..728
                     /locus_tag="PC479_003324"
     CDS             75..728
                     /locus_tag="PC479_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2805840.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            869..2140
                     /gene="brnQ"
                     /locus_tag="PC479_003325"
     CDS             869..2140
                     /gene="brnQ"
                     /locus_tag="PC479_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2805841.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2342..3529
                     /locus_tag="PC479_003326"
     CDS             2342..3529
                     /locus_tag="PC479_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2805842.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3759..5783)
                     /locus_tag="PC479_003327"
     CDS             complement(3759..5783)
                     /locus_tag="PC479_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2805843.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         2092 a          710 c          929 g         2217 t
ORIGIN      
        1 tcaaatatgt atattaagtt aatatattaa aaaaaataat atcaaaaata atataattta
       61 tagtggaggg gaaaatgact aaaattatta aagtgcaaaa acctaaattt acaggagaat
      121 tagaaattat tgaaaatctt gaagatatac ttgaagatat atttaatgat gaaaaaatat
      181 ttaataaaat tatagaaaat gaagttataa gtgatttaga ttctgtaaga gttagctttg
      241 aatcttgtat atttagaaat gtgtcttttg aaaattgtaa tttaaaaaag attgatttac
      301 ttgatgtggt atttgaaaac tgcaatttat caaatacatt ttttgatgga ggaagtatat
      361 acagagttga atttaaaaat agtaaattga taggagttag atttgatgat tgtatactta
      421 aaaacgtttt gttcaaggat gttttaggta agtattctaa cttttcattc tctaaattta
      481 agatagtttc tatcttagat tcaaattttg aaaattctgt atttcaagaa gtaaaaggtg
      541 acaatctagt atttgagtat acaaatttaa aaaaagcaaa ctttaatgga actaaacttt
      601 ctggcataga ttttactact aataatattg agggaattga gattggtata gatgatatta
      661 gaggagcaat ttttgatgtg tcacaagcaa ttgatttaac gaaattgatg ggaattatca
      721 taaaatgaaa aaaatattca taaaataaat tgggaaaacg ttaacaatgt gtgaattttt
      781 accagattga catttttctg aaaataaagt aagattatct taatattcaa aatattttca
      841 aagggggtaa tttagtgtga gtaagcagat gagagatatt tttatagttg gttttgcttt
      901 attttctatg ttctttggtg caggaaatct tatgtttcct ccatttttag ggatggaatc
      961 cggcaaagac tggttaattc ctcttattgg atttgttgtt gcagatgctg gtatagcaat
     1021 gcttgttatt atagcaacag caagatgtaa gggaagcatg gatgatgtac ttaggagagc
     1081 tggtaaaggg ttagcaagag taatgagtgt agctgctcta gcatgtttag cacttcttgt
     1141 aataccaaga acttgcgcaa caacatatga aatgggtatt atgcctatat ttggagacca
     1201 tggtccagta gctaaagctg tattttcaat aatcttcttt gggctaactt tagcatttac
     1261 tattaggtct tctaaggtta tagacataat aggtaagtat ttaactccag cgttattatt
     1321 agtattattt gttttaatag taaagggtat aatttctcca gcaggaccta tgagtcctga
     1381 gcatatgata gataaaaatc tatttggtga aggtatatca caaggttatc aaacaatgga
     1441 cgctctaggt gcagcatcta tggcaactat aatgttgatg tctataatag ctaaaggata
     1501 tacatcagat aaagaccaaa taggaatgac tattaaagct ggttttgtag cctgtgtatt
     1561 tctagcagta gtatatggtg gtcttactta tttaggtgca acagtttcaa ctttatatga
     1621 tactagtata cctcaagcat ctttattagt tgaaataact agtatattgt tatatggtca
     1681 agtaggaaag gtaattttag gtgtaattgt ggcacttgcc tgtctgacta cttctacagg
     1741 tttaacagca tcaatatcaa cttattttga aggaataagt aaggcgagtt ataaacaact
     1801 ggttataggt atatgtgtag ctagtatgct tatatcaaat ttaggtgttg attctataat
     1861 agccatatca gttccaatcc tacaaactat atatcctgtt ttattagcaa tagttgttat
     1921 ggagttggct ggtaaacata taaagaatga taatgctttt aaaggtgcag catatgttac
     1981 attagtgatt agtttattat cagctataaa tggaatgaca ggagctgttc catttataca
     2041 aagcttgcca cttgcaggtt tgggctttaa ctgggtaata ccagctatat tgggtggaat
     2101 agttggtaac tttataaaaa gcaacaaaca agtagcataa taatttgtaa ttaatttaat
     2161 tttataatat attaattttt agtactatgg gtaggtttaa tctaaaccta atctacccat
     2221 agtacttttt agattggatt ataatagcat gtatataatt ttttataata taatttttct
     2281 ttgaagtatg ataaaattag taatagtgat aagaatgtac aaatttagga ggttttaatt
     2341 aatgcttaga aaaataacag catttatgct atccatatgt ttaattattg gacttacagg
     2401 ttgtagttta cttgtaggca agaaagataa agagaaaatt atagatgatt ttaaagttgc
     2461 agtggtaact caaccattat ctgaaaataa agtacaatac aatatggtgg aagagatggc
     2521 aaaagaatat gaggaagaga ataaaattga taaagacaaa gatggtcaaa ctaaagttaa
     2581 acaaactata aagcatgttg tattgccaga gaattttact tctaatatag acagtgctat
     2641 taataagata gtaaagttgg ctgacgataa agaagttcag gctattgtag tttcaactga
     2701 ccaagcaggg cttttacctg ctttacaaaa ggtaaaggaa aagcgtccag aaataataac
     2761 tatttctgcc cctatgggag atgataaaaa ccaattaagc caatttgttg atgtgaatct
     2821 aggggttagt gcagaggaaa gaggaaaagt attagctgaa cgttcaaaag aaatgggagc
     2881 aaaagctttt attcattatg cctctacaga tgatttgaaa gatgttaata tagccaaaag
     2941 attagaaatg attaaagaga cttgcaaaaa tataggattg ccatttgtac aagttaatac
     3001 tccaaatata aatactgaag aagacaaaaa caaagttaag cagtttttaa atgaagatat
     3061 tgaaaaacaa gttaaaaaat atggaaaaga tataaatgta tttggtgtaa atgagtatat
     3121 ggatgaagtt atattgacta aggctttaga attaaagtat atagttgcag agcaaagcaa
     3181 tccatcacca atacaaacat atcctagtgt tatgggtcta aaaatatctg aaaaagatgc
     3241 tcaaaattat gataaaataa acgacatgat aagtgaaaag gctaaggctt ttggcatgtc
     3301 aaatagactt ggtggatatc ctatgccaat ggatgctttt ctaccatcag tagcaattta
     3361 tcttgctact gaaatggtga aacaagattt aacacaagaa gatgtatgtg acccagatta
     3421 tttagaggcg tttacagaat taagatttgg aatcggttca gaatttactc ctttaacaga
     3481 ggtactttat aattatcaat cagtaatttt aagccaatta atttattaat attaaggacg
     3541 tgtctcaaaa tgaacttttt agtttatgag gcactctttt tctgttaaat caaatatatt
     3601 tttatctttt taacaattaa aaagaagctt atccatatgt tgagatagct tcgtttttaa
     3661 tataaataag ttttattatt ttataaaaaa tttatagaaa aataaaaaat tataattata
     3721 tataaatttt gataaagtaa agatgaatta ctataggttt aaattatatt attttctatt
     3781 atttttatgc ctttctcagt tattgatgag ccttttcttc ccttactaga aattaagtat
     3841 ccattgcgtg tcaaatattc tagcatattt cttacttcgt attctgtgac attatattcc
     3901 ataaatttca ttaactttgt cctccccatt ggtttcatgt gcaatgattt taggatagaa
     3961 atgcatatgt ttttgtcata ttggtttaaa tcattgaata tggacatatt acttgacata
     4021 tgattattat ttaattttga agatatgttt tgaggtaaat catttattgt aagataattg
     4081 gaacccatgt aagccatata ttcaacacaa tttctcagtt ctctaatatt ccccatccaa
     4141 gtgtatgaat ttaaagcttc taaaagactc ttatcgagtt ttatgttttt atagggaagt
     4201 tcttttaaaa atgattctaa aagaagaggt atatcttcta atctctttct aagaggaggt
     4261 aaatctaaag tgaacatact tatcctataa aataaatcca tcctaaattt attttctata
     4321 attaatttct ctaaatcttt atttgtggca gctaatattc tcacatcaat tggaataata
     4381 cttgtaccac caatttttct aatttctttt tcttgtaaga ctcttaaaag tttaacctgt
     4441 aaatcatgtg gcatatctcc tatctcatct aaaaatatag tgccagtatg agctaattcg
     4501 aataatcctt tttttccacc tttttttgct ccagtaaaag aaccatcttc atatccaaat
     4561 agttcacttt caagtaattc acttggtaat gaagcacaat ttattgctac aaaaggttta
     4621 ttttttctat ttgaatgatt gtgtattgac tgagtaaagg cttctttacc tgttcctgtt
     4681 tcacctgtta ttaatatagg attatctgta agagccattt tttttgcctt tttaatacaa
     4741 tcttttataa tagaactatt tccaataata ttattaaatt tatatttagc tacataacct
     4801 cttttgacag aatcacttct tattttttgt tctaatattt ctatattgtt tacatctttt
     4861 aatgtaatta agcaaccctc tatattttta taaagataaa atggtttttt agaaagtatg
     4921 aattttttat ctatttcgtc tatgtatata atcatattat ctatatttaa gttctttgta
     4981 attttatcta agaaactctt tggcatatat tccattaagg aaggtgattt tataagtttt
     5041 tcatctagtt ccaacatatt aagtaaagac ttatttacaa aagtaacctt attaaaggta
     5101 ttgaaaaata ttagaccatc acctatttca tcaatggtca tgtaaagtat agtttttaat
     5161 ttagatatat tatctaatga tgtatttaag aaatcatgac ttagtgtctc cttggacaat
     5221 ttgtataatt tttctataaa tttctcattt tttagtttta atactactaa taatgacata
     5281 tatgtatcta atgagatttt tctccaacct aaatcaacaa tattttttat gtattttggt
     5341 ataaatctaa tctgacctgg tgttatagct gtatctaaat catatatttc atcacaatct
     5401 ggatatgtcg gtacaaaatc tatatgattt ataccaattt tatatagata agtagccatc
     5461 tcaaatgctg aagttttatt ggttgtcata agtaacgatt ttgtacctgg aggcagtttc
     5521 atcatttttt catatccaag ttttgaaagt gctttcgttc catgaataat ttcaatatct
     5581 tccttaaaat gttttattaa aaaattacta gttaaaggtg atagagaaag cactacatct
     5641 ccctctaaat tttcttttat tccatcaagt atattttgtg tttctatgat tatttcgtct
     5701 ccaaaaaagt ttgttaattc ttctttgtag tggtcactat tcaatttatc atatgttata
     5761 aggattaact tctttttatc cattttattt ctcctaacaa aatagtattt atttttaaag
     5821 tatttaaagt ataaggcaag ttatattata caacaaatag tagtataaat ttatatattt
     5881 gattaaataa taggtttttt taggatttta tcctataaaa acctattttg aaattatttt
     5941 tgcaagat
//
LOCUS       DAMJYX010000159         5941 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJYX010000159 DAMJYX010000000
VERSION     DAMJYX010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.164"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            134..700
                     /locus_tag="PC479_003328"
     CDS             134..700
                     /locus_tag="PC479_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2805844.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            740..1321
                     /locus_tag="PC479_003329"
     CDS             740..1321
                     /locus_tag="PC479_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2805845.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1378..2295)
                     /locus_tag="PC479_003330"
     CDS             complement(1378..2295)
                     /locus_tag="PC479_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2805846.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2425..4056
                     /locus_tag="PC479_003331"
     CDS             2425..4056
                     /locus_tag="PC479_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2805847.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4059..5006
                     /locus_tag="PC479_003332"
     CDS             4059..5006
                     /locus_tag="PC479_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2805848.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5291..5554)
                     /locus_tag="PC479_003333"
     CDS             complement(5291..5554)
                     /locus_tag="PC479_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2805849.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5653..5898)
                     /locus_tag="PC479_003334"
     CDS             complement(5653..5898)
                     /locus_tag="PC479_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805850.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2143 a          810 c          978 g         2010 t
ORIGIN      
        1 tattcatgta gttatataat aaataattta taaatatagc caacttagcc taaatacgta
       61 tataatatag ctaatatggt tcttatactt gataccatca ataaaactaa aaatataaaa
      121 aggagaactt aaaatgagtg ttagtttaca aaaaggacaa agagttagct tagtaaaaga
      181 taataatcct gtaaaaaatc tagtagtagg cttaggatgg gatatgaata aattgggtaa
      241 aaaaaattat gacctagatg catttgcaat agcattgact aaccaagata aaatgaatgt
      301 tagaggtgac ttggtttatt ttggaaatct taaacaccct tctaaagcta ttatacacac
      361 tggagataac ttaactggaa aaggtgaagg tgatgatgaa caaataattg ttaatttaga
      421 agacatacct gaatatgtac ataaaatagt ttttggagtt tctatataca aagctaaaaa
      481 aagagaccag gattttggtc aaataaacaa ctcttttata agattaattg attcaaattc
      541 caagcaagaa ttatttaaat ataatcttca agaagacttt tctggtaaag taacagtttt
      601 ggcaggagaa atttatcgta gaaatgaaga atggaaattt aatgctttag gaattggtca
      661 aaatgaagag cttcgtgaat taattaatac atataagtaa taattacaat aaaaaattta
      721 taatgaggag ttggtttaaa tgggtataac tttagcaaaa ggacaaaaag taagtttaac
      781 aaaaagtaac cctggtctaa aaaaagtaat agtaggacta ggatgggata ttaataaata
      841 tgatggtggc tttgatttcg atttagatgc ctctgcattt ttaactggaa ctgatggaaa
      901 agtaacgaat gatggagact ttatattcta taataattta aaacacgctt ctggagctgt
      961 tgaatatatg ggtgataata gaacaggtgt tggtgatggt gatgatgaac aaataaatgt
     1021 agacttatct aaaatacctc aaaatattgc taaaatatca tttagtgtta ctataaatga
     1081 agcaattaca agaagacaaa atttcggaca agttgaaaat tcatatataa gaatatataa
     1141 tgaagaaact aatgaggaac ttattaaata tgaattgggt gaagatttta gcatagaaac
     1201 agcaatagtt gtggcagaac tatatagaca taatggagag tggaaattta atgctttagg
     1261 ttctggattt gaggatggac ttgcaggatt gtgtaaaaat tttggcgtta atataggcta
     1321 atatatctaa ataaaaataa gagtatattg atatactctt atttttattt agatatatta
     1381 taaactgttt acacttctac tcttctttct cgcttgtttt ttcccatatt ctactgaaat
     1441 atcaaaaaat gctgaaatat tttctgtaaa aaacttgctt ctatgatatg caatatcaaa
     1501 tgtcctatgt aaattaatac cttcaatatc acaagcccac aaaagatgct tttttaaata
     1561 atcctcaata agtaattcgg atataactgc aatgccaaaa ttatctacaa ctgctcttaa
     1621 tatagcttca gagttataac atgaccatct aatattcatt gggattttta attcttttaa
     1681 ttgcttttct aattgtgctc ttgtaccact tcccaattca cgcataatta atggttgatt
     1741 tgaaagttct gaaactttta tagagtctct tttataaaac tcatgcttgt gtgaacatat
     1801 aagtacaaga ttatcattaa taatgctttt tgtaacaata tctgggtgct taatttttcc
     1861 ttctacaatc gcaatatcta attcactatt aagtaatttt tttgcaatca gtcttgtatc
     1921 ttctacaaat acttcaggtt ctataagagg atttaccttg tataattcta acattattgg
     1981 acttattaca catgtaccaa cagttactgt tgccccaact cttatacgga tattttttgc
     2041 acaatgcttc atacaatcat tcatttcttc atttacagct aaaagatgcc tcccatatcc
     2101 aagaagtttt ttccctgcct cagttaaata taattttttt gaaattctat caaatagctt
     2161 aacaccatac tctttttcta tttctgaaat agcttggcta actgatgact gggtaatata
     2221 catatttctt gctacttcac tcattttacc acattcagca accttaacaa agatttcaag
     2281 actacgaatt gtcatattat cacaacctta gcaaacatta gtaaatgctt atgattttat
     2341 taatatttta gtattttaca aatatattgt caagaagtat aattttatta tagagttata
     2401 aagtagattg gagtgtgata tattgtgaaa gtattaatta taggtggagt tgctgctgga
     2461 acaaaaactg ctgcaaaaat aaaacgtgaa atgggtgata attgctctgt tacaatacta
     2521 aataaaggtg atgatatttc ttatgcaggt tgtggtcttc catattacgt tggtaagata
     2581 attgaagata aatcatcttt aatcgtaaat acacctgaaa gtttttctaa actaacagga
     2641 gcagaagttc gttgtggagt tgaagttaca tctattgata gaaatgcaaa acaagtaact
     2701 ttgaaaacgc tttcaacagg agaaaaagaa aatctaagct atgacaaact tgtaattgct
     2761 acaggggcag acccaataaa accaccaata cctggaatag atttacctgg agtattcttt
     2821 atgcgtactc caaatgatgc tataaaacta agagattcta ttgaaaacgg tattaagaga
     2881 gctgttgtta ttggtggagg tttcattggt cttgaaattg ctgaaaatct ttctgcaatg
     2941 ggtatacgtg taagtgttgt tgatatggct gaacatgtta tgcctggatt tgatactgat
     3001 tttgctgaat acgttgaaaa tcatatggct gataaaggca taatgatttt tacaggtgac
     3061 caggttgtag gtattgaagg tgaaaataaa gttgaaaaac ttcgtactaa aaatagagct
     3121 attaaaacag atttagtggt tatgtcagtt ggaatccgtc ctaacacagg ttttctaaaa
     3181 gacacaggtt tagaatttgc ccctaataat acaattattg cagatgaata tatgcttaca
     3241 aatgacaaag atatatatgt agttggagac tgtgcttttg taaaaaattc tttaacaaat
     3301 aaacctacat ggtcacctat ggggtcatct gcaaatcatg agggtagaat ttgcgcacaa
     3361 aatatatctg gaaaatctaa aacttacaat ggtgttcttg gaacaacagt agtaaaatta
     3421 cctgagctca atgcaggaaa aacaggtcta agtaaagaaa tagctgaaaa agaaggttat
     3481 aatgtttgct ctgtaaccat agctactgat gataaagctc attattatcc aggtgcatct
     3541 aattttatta taagaatggt tgctgaaaaa aatactagaa aattattagg tgttcaagtt
     3601 atgggacctg gagcggttga taaaatagta gatatcgctg ctactgcaat aactttaggc
     3661 gctgaccttt attcattaga aagtatggac cttgcatatg caccaccttt ttcaacagct
     3721 attcaccctt ttgtagtggc agtaaatgtt ttacaaaata aacttaatgg agaacttgat
     3781 agtgttttac ttgacgaaat tgaggatttt gacagttgga ctaaacttga tgtttcaaaa
     3841 gctccttcta ttcctagttt acgttatatt cctgttggtg aaattaatgg agaaattgaa
     3901 ggtcttgcta aggatgaaaa aattcttctt atatgtgcta aaggtaaaaa tagctatatg
     3961 gcgcaaaatc gcttaagacg ctttggctat acaaatacga aagttcttga aggtggaata
     4021 ctattttatc caaatcttaa ggtaatggag gagtaaatat gagtaatgtt acagcagaac
     4081 aaaaaaagga agtaaaaggt agaggttttc taccaagcag ggatggagaa cattttgcag
     4141 cacgtataat aactgtaaat ggtgtaataa atgcatcaca agcaaaaaaa attgcagaag
     4201 ctgctgaaaa attcggaaac ggtcagttag catttactac tagactaaca gtagaattac
     4261 ctggtgtaaa atttgaagat attgaagctc taagtgaatt tattgctagc gaaaatctaa
     4321 taacaggtgg aactggatca agggttcgtc ctgttgtggc ttgtaaaggt actgtttgtg
     4381 ttcatggtct agctaataca caggcacttg ctgctgaaat acatgaagaa ttttacaaaa
     4441 attggtatga tgtaaaacta cctcataagt tcaaaattgg tgttggtgga tgccctaata
     4501 actgtattaa acctgaccta aacgatcttg gaattgttgg tcaacgtgta cctgactatg
     4561 attcagagct ttgtgttggt tgtaaaaaat gtgctgttgt agaggtctgt cctgttaaag
     4621 cagctaaatt aacagataaa ggtaaacttg aaatagatag taatctatgt aataactgtg
     4681 gtaagtgtat agaaagttgt aattttgata gtattgaaga aaaagaatct ggttataaag
     4741 tctatatagg agggaaatgg ggaaaatcag ttcgtcctgg aacacagatt gatagattat
     4801 tttcaaaaga ggaacttatg actttaattg aaaaagcaat tcttttattt agagaacagg
     4861 gtaacacagg tgaacgtttt ggaataacta tcgatagaat cggtgctgat aaatttatag
     4921 aaatgctttt atcagatgaa gttttggaaa gaaaagaaga gatactagag gctcttttac
     4981 accttacagg tggagctgta tgctaacaat aaatatttta attgtacaga taacctatgg
     5041 aaaaaatttt agttaagaat aattaaaatt ctatatatga tatatgataa atgaggctat
     5101 ctcaaaacac agatacgcct ctttttcatt gttgaaatga taaagtatat ttgagttcat
     5161 ataaaaaaga gtgtctcatg aattataaaa ttcattttga ggcacccaca ttattttata
     5221 ataattgctt attaatttta ttttaactat ataaaatgta caaaaattaa gaatatccaa
     5281 tattctaaaa ttaattttga attgtattga ttgaaaagtt tactattcca ctatttccat
     5341 cttgatttac tactagatac catgttccag tattaggaac tctcattcta catggatttt
     5401 tctttgcaaa tccaccatag taatcataat ttctttgatt tttaaatttc acataattta
     5461 tatggtctaa caatattaca ttggcttgtt tctctaaatt tacctctaca atatcccctt
     5521 tttttaagtt catcttacta taactatatt ccattgaatt actcctccag tatcgaataa
     5581 tctttgttat ttactcatct caaatatgta aaaattcatt taaaatatta caaatactat
     5641 ataattagac tattatccag ttttaacctt atttactaca atccaaaggt tattaaaaat
     5701 attattggca acaacttctc catcccatac aactctttct tctttatttt ctaataaagc
     5761 atagtaccca gagtcagaac tttcatcttt atagactgta acataaaact cttcatcact
     5821 atattcaggt atatttttaa agtcttcaga tattttacat gttaatatat aaccttctaa
     5881 ttcttcatct ataaacaaag caattcctcc aatctctata tttttactaa ttataccata
     5941 t
//
LOCUS       DAMJYX010000160         5850 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJYX010000160 DAMJYX010000000
VERSION     DAMJYX010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.165"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..1094
                     /locus_tag="PC479_003335"
     CDS             <1..1094
                     /locus_tag="PC479_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3"
                     /protein_id="HDF2805851.1"
                     /translation="LNIKFIIMSATLPRLNKLGCNDDNFAYLVKNREKFFKNHLFKDR
                     VNISFEMLNSEINDIEEISEKVIEEAEIYNKILIEFIKKSSALKFYKNIKEKLKYKLE
                     DVFLLTGDDNKLERKKIINKIKDMNSVILVATQVVEAGVDIDMDLGFKDISTIDSDEQ
                     FLGRINRSCKKLNSKVYFFNLDDASKIYKKDVRKERHLTLNEESNRKIILDKDFEKYY
                     DKIIARIEENKHKHNDKNIEVFIKDIVGNLNFTEVKKWMALIPDLKEYTIFLNTIVKD
                     ESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKMSEIMEKLDYFTYKVQKFDNSFNDLV
                     GDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   1284..4219
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1284..1313
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            4304..4798
                     /locus_tag="PC479_003336"
     CDS             4304..4798
                     /locus_tag="PC479_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2805852.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            4833..5831
                     /gene="cas1b"
                     /locus_tag="PC479_003337"
     CDS             4833..5831
                     /gene="cas1b"
                     /locus_tag="PC479_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2805853.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
BASE COUNT         2461 a          566 c          900 g         1923 t
ORIGIN      
        1 tattaaatat aaaatttatt ataatgtctg caactttacc tagattaaat aaattaggat
       61 gtaatgatga taattttgca tatctagtaa aaaacagaga aaaatttttt aaaaatcacc
      121 tttttaaaga tagagtaaac ataagttttg agatgttaaa ttcagaaatt aatgatattg
      181 aagaaatatc agaaaaggtt atagaagaag cagaaatata taataagata ctgatagagt
      241 ttataaagaa atcatcagca ctaaaatttt ataaaaatat taaagaaaaa ttgaagtata
      301 agttagaaga tgttttttta ttaacaggtg atgataataa attagaaaga aaaaaaataa
      361 taaataaaat taaagatatg aactctgtaa ttttggttgc tacacaagtt gtagaggctg
      421 gagtagatat agatatggat ttgggattta aagatatatc tacaattgat tctgatgaac
      481 aatttttggg aagaataaat agatcttgta aaaaattaaa ttcaaaagta tactttttta
      541 atttagatga tgcaagtaag atatataaga aagatgttag aaaagagaga catttaactc
      601 ttaatgagga aagtaataga aaaattattt tagataaaga ctttgaaaag tattatgata
      661 aaataatagc cagaatagaa gaaaataagc ataaacataa tgacaagaat atagaggttt
      721 ttataaaaga tattgtggga aatttgaatt ttacagaggt aaaaaaatgg atggctttaa
      781 taccagattt aaaagagtat acaatctttt taaatactat agttaaagat gaatctggaa
      841 atatgattgt aggaagtgat gtatggtatg aatatatatc actactaaaa aatgataatg
      901 ttgagtattc tgaaaagagg gtgaaaatgt ctgaaattat ggaaaaacta gattatttta
      961 cgtataaagt tcagaaattt gataattcat ttaatgattt agttggagat attttttata
     1021 ttgatgatgg aagtaagtat tttacagagg gaaagtttga tagaagtaaa tttaatcaaa
     1081 atgaatttct ataatgtatt tagattatat agagaatatt tatcccaagt taattaatta
     1141 gaattagttt aaaactattg aaatatagat attttatagc ttatatattt tttctaggat
     1201 tagctgggat aaaaaatctg tatattagtg ttttcagtgt atttaagggg attatatttt
     1261 taagaagtac tgattttaaa gtggttaaac agtaacatga gatgtattta aatttttact
     1321 acataattaa atcgtagtca ataccgtaca ggttaaacag taacatgaga tgtatttaaa
     1381 tctctgaata gccaatcaac tttgctgcat tactcttgtt aaacagtaac atgagatgta
     1441 tttaaattta cttctcctgt tttgaaatct atatattcat catgttaaac agtaacatga
     1501 gatgtattta aatataatat tgatacaaat attttgggta aatggacttg ttaaacagta
     1561 acatgagatg tatttaaatt cgatatggta acaaatttat aaaagatata gaatgggtta
     1621 aacagtaaca tgagatgtat ttaaataaag aaaaacgaaa ttggtgattt tgaagcaatg
     1681 tgttaaacag taacatgaga tgtatttaaa ttagtaacta caatgctttt ccttttgaag
     1741 atgatatgtt aaacagtaac atgagatgta tttaaattaa taatgcacct attttaacag
     1801 tacctggtct aaagttaaac agtaacatga gatgtattta aattcaagtc taggagacaa
     1861 cataaatagt acagatgggt taaacagtaa catgagatgt atttaaatta gtaaaaccag
     1921 atggaactgt tgtaatacca ccaaagttaa acagtaacat gagatgtatt taaatttaaa
     1981 aacatcttga ggaatatctt taacagtaat tgttaaacag taacatgaga tgtatttaaa
     2041 tgcattttca ggtgcatgat aaataggtaa attagatgtt aaacagtaac atgagatgta
     2101 tttaaatttt ttgtaattag ccagattatc aactcccaaa atagttaaac agtaacatga
     2161 gatgtattta aattaaatat cgtccttcgt agtcaaagca acaccagaag gttaaacagt
     2221 aacatgagat gtatttaaat cgtaaacaaa atttatcaag agaagaattt ctaaggttaa
     2281 acagtaacat gagatgtatt taaatttata tttgataaga gtatcaaatc gacttaaaat
     2341 agttaaacag taacatgaga tgtatttaaa ttcaatctta atacacagac atttttaaaa
     2401 acaaaacgtt aaacagtaac atgagatgta tttaaataaa tagacaaata ttgaatagct
     2461 ctgtcttcat caagttaaac agtaacatga gatgtattta aatatattta caaaatctct
     2521 aacatatttt ttaacttctg ttaaacagta acatgagatg tatttaaatt cttttaaaga
     2581 gtcctcggga gcctcgaaaa cttcagttaa acagtaacat gagatgtatt taaattactc
     2641 aaataaattt ctaatttgtt tatctactaa cgttaaacag taacatgaga tgtatttaaa
     2701 ttagaattta caacaatata agcctcttta accataagtt aaacagtaac atgagatgta
     2761 tttaaatatg tttttagtag tacatttaat aaaacttttt aaagttaaac agtaacatga
     2821 gatgtattta aatgaaggaa ccgtaccttg ataaatcacg accgtacccg ttaaacagta
     2881 acatgagatg tatttaaatt gtatatttga tgttgttatt tctcctgtgt ataatgttaa
     2941 acagtaacat gagatgtatt taaatgaagt tccaccccca ccacccccac tactttgaga
     3001 agttaaacag taacatgaga tgtatttaaa ttaacaatag agtaaatata ataaacaagc
     3061 atacaaagtt aaacagtaac atgagatgta tttaaatccc cttttgcatg gtactagaca
     3121 cgcatatatg gtggttaaac agtaacatga gatgtattta aatacgccta tatgcggagt
     3181 aacagatttt tgcctggatg gttaaacagt aacatgagat gtatttaaat actgttttgg
     3241 gatagactgg aattaaatgg tctgtgttaa acagtaacat gagatgtatt taaatttttg
     3301 gaatgaagca tcaacagata ataaagaaaa agttaaacag taacatgaga tgtatttaaa
     3361 tcaaggaaac aaaagagaag tgaaaagaaa tataatgtta aacagtaaca tgagatgtat
     3421 ttaaataatc ttttaaatgg aaattaagta ataaaagaga gggttaaaca gtaacatgag
     3481 atgtatttaa attcaattaa ataagcatta atcttagtaa aatcaaacgt taaacagtaa
     3541 catgagatgt atttaaattt tcttgtgtta attgaacccc cattttaagc tccagttaaa
     3601 cagtaacatg agatgtattt aaatcagaat acagcttcta tatctggata catttgtctt
     3661 ggttaaacag taacatgaga tgtatttaaa tgtgaaaagt aatacaggta tagagtctaa
     3721 acgctcggtt aaacagtaac atgagatgta tttaaatcaa tgtatcctct tttttagttt
     3781 tggagcctct tttgttaaac agtaacatga gatgtattta aattagttag cacattttat
     3841 ttcaagcctt aaaacattcg ttaaacagta acatgagatg tatttaaatt aatttgcaca
     3901 cttaacctct aatcttaaag tattagttaa acagtaacat gagatgtatt taaataacac
     3961 aatattatct ttaatagaat taaacaaatg agttaaacag taacatgaga tgtatttaaa
     4021 tttttgttgg tcttgtgctt cttttaaaag ctgattagtt aaacagtaac atgagatgta
     4081 tttaaattta aaaagttctg ttattaaaac atccaaacca atcgttaaac agtaacatga
     4141 gatgtattta aattctgtac catctagcca aaccgttttt gaagttaaag ttaaacagta
     4201 acataagatg tatctaaatt actctatcat aaaattttaa tatattaaac aaactaatat
     4261 gtgatatact tatgtataaa taacaaaata aataggtgat gttatgaaaa ttacaggaac
     4321 tttgataaat tactatttcc attgtaaaag acaatgctgg ttgcttggta atagaataaa
     4381 tttggaagaa aatagtgaag atgtaaaaat aggtagactt cttcatgaac taaaagaaga
     4441 aaaaacaaaa tataaagaaa ttgcaattga aaatataaaa atagacaaat taacaagaga
     4501 atatttagta gaggttaaaa agtctgatgc tgatatagaa gctgttaagt ggcaagttct
     4561 tttatattta aaaaagttaa aagaaaaggg aattgtaaga aaagggaaaa ttgaatttat
     4621 agaaaaaaat aaaactaata aaaaaatagt ctttgtagat ttgagtgaag tatctgaaga
     4681 acagcttata tctatagaaa gagaaattgt taatttaata aatagtgata aagcaccatg
     4741 tcctgattta aaaaaaggat gtaaaaaatg tgcctattat gaatattgtt atatataaag
     4801 aggtataaga aacatataag gaggtgttaa tattgggaaa gacaaaatat attacctcaa
     4861 tgggtgaact cacaagaaaa gataattcta tatgttttag aaaaaatgga aggaatgtat
     4921 atataccagt agaaaatact agtgaaatat tctgtctaaa tgaggtatct ttaaattcaa
     4981 aattactgga ttttttatca agagcaaata ttgttgttca tttttttaat tactatggtg
     5041 gatatagtgg aactttttat cctaaagaat atttaataag tggaaaagta acagtggcac
     5101 aggtaaaaac ctttgaagat agaagagaag aaatagcaaa atccatagtc aatggtataa
     5161 ggattaatgc aataaataca ttatcgcatt actataagca tggaaattca gaaatacaac
     5221 cattattatc acatctaaaa aaagattcta taaacaagtt agaaaaatca aataatataa
     5281 aagaaatatt atcagtcgaa ggagatattt ggcaaacatt ttatacttcc tttaagtata
     5341 ttttaagaga ggaatttatt tttaataaaa gagttaagag accaccagat aatcccttaa
     5401 acgcaatgat atcatttggt aacagtattt tatatgctaa aactgtaact gctatatata
     5461 atacacatct aaatcaaagt gtaagttttt tacatgaacc aagtgaaagt agattttctt
     5521 taagtctaga tttaagtgaa aattttaaac atgttataac ttttaaaact atatttgatt
     5581 tagttaataa tagaaaaata aatattgaaa aacattttga taaaaatttg aactactgtg
     5641 ttctaaatga tgaaggaaag caaattttta taaattcttt agaagaaaga ttagaatcag
     5701 tcaaagaaca tccaaaatta aaacgaaaag taagtttaaa aacctgtata aaattagatg
     5761 cttacaaaat gttaaagtgt ataatggaag aaaaaagttt tattccatat aacataaaag
     5821 acggattata atttaaacat aaagtaagta
//
LOCUS       DAMJYX010000161         5753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJYX010000161 DAMJYX010000000
VERSION     DAMJYX010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.166"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            136..1248
                     /locus_tag="PC479_003338"
     CDS             136..1248
                     /locus_tag="PC479_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2805854.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1456..1932)
                     /locus_tag="PC479_003339"
     CDS             complement(1456..1932)
                     /locus_tag="PC479_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805855.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2131..3756)
                     /locus_tag="PC479_003340"
     CDS             complement(2131..3756)
                     /locus_tag="PC479_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2805856.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4094..5113)
                     /locus_tag="PC479_003341"
     CDS             complement(4094..5113)
                     /locus_tag="PC479_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2805857.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5110..5679)
                     /gene="mocA"
                     /locus_tag="PC479_003342"
     CDS             complement(5110..5679)
                     /gene="mocA"
                     /locus_tag="PC479_003342"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2805858.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1963 a          967 c          592 g         2231 t
ORIGIN      
        1 atatttgaca taatatatac attttttaaa taaatatata caatgtatta aaatgtatgc
       61 aactctattt caaaattatt ttgtgatata tatatgttat aataaatgta taaaattttg
      121 attggtgggt tatatatgaa tgcatttatt agaaaaagaa ataaaaacta tgttgtttac
      181 ctggaatttg aagatgaaaa gacaggtaaa agaaaacaaa aaaatatggg tacttttgat
      241 aaaaaaaggg aagcaagtaa aagactaaat gaggtaaaag aaagcttata taatgatggt
      301 tttttagtac caagcgaaat tacagtgtca gaatttctat tagattttct aaataaatat
      361 agtgaaaata tatctttagc tacatataat aattatgtta gtatttgtaa gaattacatt
      421 aatccatcta ttggaaaata taaaatacaa gacttacatc cggttcacat acaaaactac
      481 atagataaac tatcttataa actaaatcct caatcaatta agattcatat aaatatattg
      541 aaacttgcaa taaaaagagc ttatagactc aaattaataa aagaaaatgt tatggatagt
      601 atagaggctc caagatataa aaaatttaaa aatgagattt atgatagaga acaaatgata
      661 aaattattag atttagcaaa aaatacagaa atggaattac ctattaattt ggctattgga
      721 ttaggtctta gaatttcaga agttttaggt ctaacttggg ataatattga ttttgaagaa
      781 aatacaataa cagtaaataa gataacttcc cgaattaatg gttctgttat tctcaaagag
      841 ccaaagacag aaagctctgt tagaaaaata tcttctccca aagaattaat gtctttatta
      901 aaagaatata aaataaaaca gaataaaaat ttattaaaat cttccattag aaataataat
      961 aatttgttat ttttcaataa gaaatgtgaa cctattgctg aagatgttat gagtaaaaaa
     1021 tttaaaagat ttttagaaaa gaatgaatta cctcatatta gattccatga tttaagacac
     1081 tcacatgtta ctttacttat aaattctaaa gtacctataa aggttatatc cgagagagta
     1141 ggacactcaa atataagtac tactctaagc gtttattctc atgtacttaa agaaatggat
     1201 aaagaggctt ctgacaaaat ttctgaaagt ttatttaatg ctaattaata ttaatttatc
     1261 ttagctttaa attagcttag cttattgaga tagattcatt aatattaaat gtatctatct
     1321 caaattaata actacttaaa taatctgttt tattttaatt tatcgcctca gttattatac
     1381 ttttttacga aaaattatac tttacaataa aaaagatatt cccttataga aaatatcttt
     1441 ttaaatacta tattattata tacttgctaa cattggaata tctaacatct tcttcattac
     1501 atcatctact ttttttgctt tatattcatt gtatccaatt aaatatcctt tttcatatga
     1561 actttttaaa atctcttttt catataaatt atcagatttg gcttctgtga cacttaaatt
     1621 ataaagccct ttacacccat cttcataacc tttattatac cctttttctg caaaatagtt
     1681 tttatgattt ttttcgttat gtactgaaga tattgttact ccagaatttt cttttgtact
     1741 agcataagga caaaaacctt tattgtgttg atgtgctgaa tgtcctccac aataataacg
     1801 tgttaactct gatttatttt tattatgatg acctcctgat gaattatgcc ctcctgagtg
     1861 agcatatgaa aaattcccta cactaatcat agctaaacta attactacag ctactaacct
     1921 tttcttaacc ataatctatt ccccccaaaa tgttatatat ttttattata tcatctacta
     1981 tattatattt aaattcttaa tatacaaaat attactctat tctgctaata aacatgttta
     2041 aacaatataa aaaacctcct aatatttgat taaaaattta cacactcatg catagcatgt
     2101 ctcaaatatt aaaaggtttt atactaataa ttatttatct tttccataca ttttagctaa
     2161 ttggtcttgt atctctttac tttctaaata ctcatcaaag tccatttttc tatccattat
     2221 tccatttgga gttatctcta taattctatt tgctatagta gatataaatt catggtcatg
     2281 agaagtaaat aaaagcactc ctgggaactt ttcaagtcct ttatttacag aagttatact
     2341 ttctaggtct aagtggttag tagggtcatc taatacaagt acatttgcat ttgaaagcat
     2401 tagcttacta agcatacatc taaccttttc tcctccagat ataacttgag cttcttttaa
     2461 tgcttcttca cctgagaata acattctacc taaaaatcct cttataaaac tttcactttt
     2521 ttcttcagag aactgtctaa gccagtcaac caaagaatat tctactccgt caaaaaactt
     2581 attatgattt tttggaagat aatcttgaga agtagttact ccccatttat actctccact
     2641 gtctggttca agttctccca taactatgtt taaaagtgca gtagtagcta tttcatctcc
     2701 cataaatact actttatcat ctctatccaa tctaaatgac acattatcaa gaaccttaac
     2761 cccatctata gttttagtta aattgtgtac ttctaaaact tcattcccaa tttctcttgc
     2821 aggtgtaaat cctacatatg gatatcttct tcttgatggt tgtatatctt caacttctaa
     2881 tttctctaac tgtttctttc ttgatgttgc ttgtttagcc tttgaagcat tagaactaaa
     2941 tctagctata aattccttta attgagctat cttttcttca gtctttttat tttggtcctt
     3001 agccaattgt aaagccaatt gacttgactc ataccagaaa tcatagtttc caacgtacat
     3061 ttgtatttta ccaaaatcta cgtcaactat attagtacat atttgattta ggaaatgtct
     3121 atcatgtgat actattataa ctatagattc ttctaaatcc attataaaat tatttaacca
     3181 gtttattgat ttaaaatcta agtggtttgt aggttcgtcc attaatagta tttcaggctt
     3241 accaaaaagt gattgtgcta acaatacctt aaccttttcc ccaccaacta gttccttcat
     3301 ttgtttgtaa tgcatatctt tcgttatgcc aagacccata agtattttct cagcattagt
     3361 ttctgcatcc catccgtcaa gctctgcaaa ctcaccttca agttcggcag cttttatccc
     3421 atcttcttca ttaaagtctt ccttcatata caatgcatct ttttctttca ttatgttcca
     3481 aagtctctca tgacctctta taacaacatt taaaacagtt tcttcttcat attcaaagtg
     3541 gtcctgtttt aaaactgaca ttctttcttt atcagttata gatatactac ctgtattagg
     3601 ctctatttct cctgataata tttttaaaaa agtagatttt cccgcaccat tagctcctat
     3661 tattccatag caattacctt tagtgaattt taagttaaca tctttaaata gttccttatc
     3721 accaaatcta agtccaacac ctgtaacttg taacattaat atattttcct ttctttttgc
     3781 tcataattta ttttatcata aaactttgat aacattttta ttatatatta tcacgtaaat
     3841 agtatatttt ttatactaaa tttgtgaaat atttattaaa ataatactaa aaattgtaaa
     3901 tttataaaaa ttaacaatct cttatattat acttataaag aaatcaaatt gcaactttat
     3961 tcattctttt acacattgaa aataagttat tataatttct aaataaatat gcttttttta
     4021 aaacatcatt ctagctgttt tgtttcatat ttaaaaagag tacttaattt gtaaatactc
     4081 ttttttaata actttatatt ttagcaaaat tacatgcagg ataagtacat acatcacagt
     4141 tcatacaaag gccaccatga ccatatctag ctatatcata cttatcaagc ttttcatcaa
     4201 ttaaaaccct agataaaact atatcaaata ctgtagtttt gtgatacatt gcgcatcctg
     4261 gtatacccat aattggaact tctccgtaat aagcaagtaa aaacatagct cctggtaata
     4321 ttggtgaacc atatgtcact aaatcagctc cagtttcttt tatagctgtt ggcgtaacat
     4381 catctgggtc tacactcatc ccaccagtac aacagataag ttctgctcct tgacttataa
     4441 attcttttat tgcactcttt ataatttctt tatcatctgg gcatatagtc tgtcctataa
     4501 cttcacaacc aaagcctttt actttttctt ctattactgg tccaaattta tcaacaattc
     4561 tgctataaaa cacttcattt cctgtagtaa caattcctac tttcttaggt ttaaaaggta
     4621 ctacatttac tatttttcta ttaccaacta ctttttttgc atcttctaat tttttctcat
     4681 ctataactaa aggtatcact cttgttccag ctacttttaa acctttattt actataaaat
     4741 tattatgaag agtagccatc attatttctc caacacaatt caaatcaaaa agtgcatcaa
     4801 catctatctt taatagacca tcacatgctg ctataaaatc tattttccct tcttttattt
     4861 cactaaaatc taaattttct ccagcagtta gttcttttaa aaacaatgcg ccttcatttt
     4921 catgaaccat tccttctgac ttttcccata catacaaatt atctttacca agagataata
     4981 acactggaat atcttcttct tttactatgt gacccttttt aaattttctt cctttaaatt
     5041 ctccaggtat tatttgagtt atgtcgtgac ataaaacttg tccaacagca tctatagttt
     5101 ttattagctt cataatttac ccccaattct ctcatagtcc atttttgtat cgatatcaaa
     5161 taacatttct ttaggaactt ctacatactt tatttttgat gaatttttta aaatggtttt
     5221 acctttttca tcttctttaa gtgccaacaa ttcttctttc ttgctatatg ggaatatgac
     5281 tggatttcca cacatatctt tgtatcttgg aattactata aaatctctat ctaatttaaa
     5341 agtatttata attttatcta tatataagtt atcaataaat ggctggtctc ctacaaagaa
     5401 catataacca tcacattttg agctatttaa aactcctaat tttatagatt cactttgacc
     5461 tatatttgca tttttattat ctatatattc aaaaccatat ttattacaca ataacaaaac
     5521 ctcttcatat tgacttacta ctacaacttg atgaaaatca atcttgctaa cttctctaaa
     5581 tacatgttct attatagaac agcctttata atttaataaa agtttatttg ctcccattct
     5641 tctggcaaag cctgatgcca ttatcactgc atttatcata tatatcatcc ttggtattta
     5701 taatatattg atagttctat gatttttata atatctctat tgatattatc tat
//
LOCUS       DAMJYX010000162         5667 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJYX010000162 DAMJYX010000000
VERSION     DAMJYX010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.167"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            126..755
                     /locus_tag="PC479_003343"
     CDS             126..755
                     /locus_tag="PC479_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2805859.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(869..2383)
                     /gene="glpK"
                     /locus_tag="PC479_003344"
     CDS             complement(869..2383)
                     /gene="glpK"
                     /locus_tag="PC479_003344"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2805860.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2627..3169
                     /locus_tag="PC479_003345"
     CDS             2627..3169
                     /locus_tag="PC479_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2805861.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3183..>5667
                     /locus_tag="PC479_003346"
     CDS             3183..>5667
                     /locus_tag="PC479_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2805862.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENK"
BASE COUNT         2106 a          869 c          783 g         1909 t
ORIGIN      
        1 aaatattatt ttaaaacatg cacaatattt gtacatattg tagacatata aacctataaa
       61 ttatacaata gtattgttaa gattatatat ttttttaaca taaattttgt attgggggaa
      121 aaacaatgga taaaaactat acgatgccaa tataccaaaa aatagcgttg gatattgcaa
      181 ataaaatata cacgggtgaa atacaagagg attcagtact atttggacgc tctgttttgg
      241 ctggtaaata caatgtatct ccagaaacga taagaagagc tgtcaaaatt ttagaggaca
      301 ttggcgttgt taaaagcata aagggtaaag gtgttattgt actatctcca gacaaagctt
      361 cttcttttat aaaaaaatat agagatatta caaatatatc atcatataaa tcaacacttt
      421 ataatttaat agatactaaa tctaatttag aaaatgaaat attagatact ataaataaaa
      481 ttttagacta ttctaacaga cttgaaataa tcaatccttt agtgcccgtt cagttcacca
      541 taaattctaa ttgcaaatat attggtcaaa cagctgcaca aactaaattt tggcaaaata
      601 caggtgctac aatagttgca attaaaagag gtgaagaact tataatatct cctggaccat
      661 atattgaatt tttagaagga gacattctct tagtagttgg tgaccaacac atttacaatt
      721 ctataccaat gtttttatat gaaaacgaaa aataaattat ataatttagt gtgattatat
      781 aaaaatgcaa gattaacaac aaaaaaagag cttcatattt tacataaagc tctttttaaa
      841 ttcaaataaa tttaactttg attaattttt aaatatccaa ttcatcttct ttttcccagc
      901 ttaaagctct cgatacagct tttttccatc ctttatataa tttacatctc ttctcttcac
      961 tcatatttgg attaaactct ttttctactg accactttga agtaatttca tttcttccaa
     1021 tatagaatcc aacagacaac cctgctaaat aagcagctcc taaagctgta gtttctacta
     1081 ttttgggtct atctatatct acatttaata tatctgattg aaattgcatt aaaaaattat
     1141 tattacttgc tccaccatca acttttaatg attttagttt taattttgaa tcgttttgca
     1201 ttgcttctaa aacatccttt gtctgataag caattgattc caatgtggct cttactatat
     1261 gttctttttt tgctcctctt gtaagtccta aaattgaacc cctagcatac atatcccaat
     1321 atggagctcc aagacctgta aatgctggca ctacatatac accattagta tcctctaccc
     1381 tatttgcata catttcacta tcttttgcag attcaattat ctttaattca tctctaagcc
     1441 attgaacaga tgctcctcca ataaatatac ttccttctaa tgcatatgtt acttttccat
     1501 caactcccca agcaattgtt gtaagtaacc catgcttaga tttaactatg ttactgcctg
     1561 tattcataag caagaaacaa cccgttccat aagtattttt tgcagtacct tcttcaacac
     1621 aattttgacc aaataatgct gcttgttggt ctccagcaca tcccgcaata ggtattttag
     1681 ctcctgataa catgcgctca tctgtatgac cataaacata gctagaaggt tttactaatg
     1741 gcaacatgct tattggtatg tccaattctc gtaatatatc ctcatcccat tctaaatcat
     1801 ttatattgta catcattgtt ctagatgcat ttgagtaatc tgttacatgt acttttccac
     1861 gagttaaatt ccatattaac catgtatcta tagttccaaa taataactct ccttgttctg
     1921 ctttttccct agctccttct acattatcta atatccattt tacttttgtt gctgaaaagt
     1981 atgcatctat taaaagacct gttttttctt ttattgattc ttccaatcct ttatttttaa
     2041 gttcatcaca tatttctgac gttctcctac actgccatac tattgcgtta taaataggtt
     2101 taccagtata cctactccaa acaaccgtag tctctctttg attagttatc cctattgaag
     2161 caatttcttc tggtcttata cctgctgttt ctataacttc tctcattacc ccactttgtg
     2221 acccccatat ttccataggg tcatgctcta cccatccagc ttttggataa atttgattga
     2281 actctttttg tgcaattttc actatttcgc cttttttatt aaatagtata gctcttgaac
     2341 tggttgttcc ttggtctaat gccattacat acttcttttc catcaccata ttctccttat
     2401 catataatat ttaatttatt aaaaagaaaa atttaatatc acatatttca ataaataatc
     2461 atacttaagt acaaatttaa tataatagat atttagtaag aagacactta ttttctatca
     2521 tatctatata ataatattat acattatagc ttacatattg attaataaat tttaaaggag
     2581 tataataaac agtgttcata ataattttaa taaaagaggt ggtaatatgc caaagatttt
     2641 agaaaatgta aaagaagata tattaaaagt gtctcgggat atgattttag aagaagatta
     2701 ctcgaatatt agcataagga aaatagctca aaggtgtggt ataagtgcag gtacagtgta
     2761 taattacttt aattcaaaac aagaaataat agaatatatc acaaaatctg aatgggattt
     2821 attgataagg cgaattgaat actccaataa aaacactgaa gattacataa aaaaactaaa
     2881 cattattttt actgaaataa gaaattttat aaacaaagtt cataatatcc aatacaatga
     2941 ctttttaaac acttttgaaa cagagagatt ttttgaaatg aaaaaacaca aagacgactt
     3001 tcatgaacag ctttcaaata aagtatatga agctttagaa aaagaaagtt tttttaataa
     3061 tgacaaatta gtatgcaata tcataataaa aatgtttttt tcatattcaa catatcttca
     3121 tatagaattt gaagatttaa aaccctatat tgaaaaatta atacattaag gtggtgttta
     3181 ccttgaatca taatactagt attaagaaca aggacagtaa tccaaaaata tttttagtac
     3241 ttacgcttta tctattaggt atatttatgg gagctattga tactggaata gtatcaccag
     3301 caagaacgat tatacaaaat agccttggag taaatgaaaa aacgggaatt tggatgatta
     3361 ccatatacac attaacatac gcttctgtaa ttcctatctc tggaaaatta gcagataaaa
     3421 taggaagaaa atatgtgtat ctagttagta tatttctatt tggtagtggt tcattaatat
     3481 gtggattatc atcactattt tccaatttct atattttatt aattggtcgt gttattcaag
     3541 caattggtgg tggtggaatc atgccaattg ctacagcaga atttggaact accttcccag
     3601 aaaataaaag aggcatggct ttaggtttag ttggggcaac atatggtatt gccaatattt
     3661 taggttcaag cattggtagc acaattctaa gtatctttgg aactcaaaat tggaagtggc
     3721 tattttttgt aaatcttcca atttgtttaa tcatacttat tggtggagtt ttctgtatta
     3781 aaaacaataa gtctgaatct aaagaaaaaa tagataagct tggtacattg atgttgatat
     3841 gtataatcgt atcactatta tatggtctta tgaatataga cttttttaat tttaaaaatt
     3901 ctatacaaga tatatcagtt tatccatact tattgacatt tattgtactt atacctattt
     3961 tcatatttat tgaaaataga gctaaagacc ctattttaag ctttgaatat tttttaaatc
     4021 cgagaatatt gatagtactt attttatctt taattgttgg tattggaatg atgggtatgg
     4081 tattcgttcc acagtatgct gaaaatgcat taaagataaa tgctggttct gggggatatt
     4141 ttgtagctat attgggatta tttgcaggta ttgctgctcc tttaagcgga agacttattg
     4201 acaagtttgg ggcaaaaaaa atactattac taggattttc tgtatctatg ataggctctc
     4261 tttacctagt tttaattgct ctaaaaacca atactgtatt tagtgtatgt gtaagtttaa
     4321 tatttatggg attaggaatg ggatttacta tgggaactcc tctaaactat atgatgttat
     4381 caaatacaag attagaagaa tctaattcag cacttgcaac attatccttg atacgctcaa
     4441 ttggtacttc aatttctcca gctataatga ttggatttat agcacatgca ggattatctg
     4501 tgcaagataa tattatggac ttagttggaa agccctcaac tcctaaaata gttcaacttg
     4561 aagaacttga aaacatgata gatgacttaa aatcagaccc tgaaatggca aaaaatttaa
     4621 aaaatgtctc tatcccaaat atgaatgata cttcaaatat aaatatggac atgacaggtg
     4681 gcaaacttcc aaaatcctta cttaataaag ttcagtcagc agatacaacc aacattacca
     4741 atataacaaa agaaatatca acaacaatgt ttgatgaaaa agttccatct ataattacta
     4801 aaatacagaa aaatgtacaa aaaggaatag atggaacaca aagtggaata aacggtattg
     4861 aagatggtga aaataaacta aatagtggta ttaaaggagt tcaaactggt attgacaata
     4921 tggtaaaagc tagagctggt attaatcaag gaatagatgg tgtaaaaaaa ggtattgctg
     4981 gtgttgataa aggtataaaa ggtatggaac aaggtcttga aaaacaagat gaggccatca
     5041 aggaacttac aactgcttat aataaaatac ctactacaga aaaaactcct actgataacg
     5101 aaaataactc aaatgaacaa aaccagagta ataccagtaa ttctgaatca gacgacacta
     5161 agaatgattc tacagaaaat aagaataaca attccagcga gaataaacca aatgtaaatg
     5221 aacaaaaaga aagtttaaat gctcaaatac agcaactaaa aaagacaaga gatgacttaa
     5281 atacaaagtt acaaaaaaat aaaactcaaa agaacgaatt aagtaaaaaa ctaagaacaa
     5341 tggaatctca aaagaaagag ctacaaagca agttaaataa ttctataagc caaaagaaag
     5401 acatggaaaa aagccttgat acaatgcaac agcaaaaaaa atcactccaa gcagttttag
     5461 caaagacaca agaggtaaaa actgaaatac caaaggcttt tgataaatca aaattggatt
     5521 atataaattc catagaaaat aatagaacaa aaatagaaaa cacactacaa tctactctaa
     5581 actcaggatt taaacagatg tatataacag tattctgtgt aaatctacta gcatttataa
     5641 tattattgtt ctataaagaa aataaaa
//
LOCUS       DAMJYX010000163         5656 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJYX010000163 DAMJYX010000000
VERSION     DAMJYX010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.168"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            148..648
                     /locus_tag="PC479_003347"
     CDS             148..648
                     /locus_tag="PC479_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2805863.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            705..1691
                     /locus_tag="PC479_003348"
     CDS             705..1691
                     /locus_tag="PC479_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2805864.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1741..2463
                     /locus_tag="PC479_003349"
     CDS             1741..2463
                     /locus_tag="PC479_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2805865.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2492..4426
                     /locus_tag="PC479_003350"
     CDS             2492..4426
                     /locus_tag="PC479_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2805866.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4419..>5656
                     /locus_tag="PC479_003351"
     CDS             4419..>5656
                     /locus_tag="PC479_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2805867.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKND"
BASE COUNT         2367 a          430 c          972 g         1887 t
ORIGIN      
        1 atattgtata atataaaatg aattgggaaa atagttcgct aaaataattt gtttttataa
       61 aattactgtt taagtttata gattttgtat aaaataaagt tatgttatta atttttttaa
      121 aaataaaact tttactagga gacagatatg agaaaaaata gtattaatat agcactaact
      181 gcagcaatta cagcaatcta tgcagtttta acattatcat tgggatttat aagttatgga
      241 ccaatacaat ttagggtagc agagattatg atgcttttgg catttttaga taaaggatat
      301 atagtaggtc ttacattagg ttgtttttta gcaaatgtaa taggaccata tggtgtacct
      361 gatataattt ttggaacatt agccacattt ataagtgcaa gtatgattta tattactaga
      421 aagttaggta aacaaaataa aagtacttta gttatagctt ctttatggcc aactgttata
      481 aattcgattg tggttggtct aatgttaaat gtattctttg ggcttccatt aattctatct
      541 atgatacagg taggttttgg agaatttgta gtagtaacaa ttgtaggagt accatttttc
      601 tgctttttaa tgaaaaaata taaaaatgtc attaatatta aattttaaat agtgtatagt
      661 aatttaatta attgagagta ttattagtga aaagaggtgt ttatttgtcg aaggtaaaaa
      721 gattgactga tgtttggata tatattaatg ataagcaaaa gtttacaata aaagagttgt
      781 cagaggaatt taatctttca gctaaaacaa tacaacgata tttaaaagaa ttaaataaaa
      841 tgggacttcc aattcaagca gaacaaggta gaaatggagg ttatagggtg ctaaataata
      901 gctatatacc tcctgttata tttacagaaa aagaagttat gattatacta tttgcactta
      961 agtcacttca gatttataat tataagttaa tggaaataga aattaattct ataatgagaa
     1021 aagtaagtta tgaacgtaaa agcagtataa aagtaggtat agaaaatatt aaaagataca
     1081 ttggctttgt agctaatgat actaatgatg gagattttaa gagtagtaat gtaactgagt
     1141 tttttagagc ttcttcagaa tctctaattt taaatattaa gtataagtgt ggtaatcaga
     1201 ttttggaaaa aaacgtatgc cctataggta tatatttaaa taagggagca tggttttcac
     1261 cagcatatga ttgtaaaagt gataaaataa tattagttca tatagataat ataattgata
     1321 taaaaagaac aggagaaagt aaaaaaatac atataaactt ggaagaatgg ttacatgaag
     1381 tatataacaa tttatatgga aatagttcaa ctactaaaaa atttggtgaa attatttatc
     1441 taaatgtatt attaactaaa gaaggtgttt ctaaaataaa ggacagttct tggaatttag
     1501 atatggagct aaatgaagat ggtagtggaa taattagtac attcataaag gttgaagata
     1561 tagattgtta tgcttcgatt ttatttttta ttggaaataa tgctaaggtt atagagccaa
     1621 aatttttgaa taagctactt tatgataagg ccaatgagtt aagatatttt tatgaagaaa
     1681 atatggtata atatttagaa atgttgaaat taaataatta ttaaggagat atacgttata
     1741 atggaaaatt ttaataagaa tgctaaaata attggtattg gagctgaagg tattaatata
     1801 ataaatgaag tagaagaaaa aataaaagca aatatggata tagaaaaaat aaacataaat
     1861 caagagattg aaaaagaata tgttagaagt cttttagatg gtgtagatat attatttttg
     1921 atatattcat ctgaagataa acatattaga gatattataa aagctgtttc atatatgtca
     1981 aacgaaagaa gagttctatc tataggaatg gattgttcag agaaggaaaa taaagaagat
     2041 ttagagcttg ggagagagtt taaaatcaat aatgatagta tatttaaatt tgttgattta
     2101 atgaatatca tggtagagtc tatttctgat tcttgcatga taaatataga tattactgat
     2161 ttaaaagaag ctattgtagg agataaagga attaagtatt cttttgaaga gtttgaagat
     2221 acaaagtcat atagcgagat agctgacatt ttatttgaca ggatggaata tattggtgag
     2281 gaatttataa gtaagaaagg aattgtattt gttgaaggaa gtccagaatt ttctataatg
     2341 gaattgaatg atttaataag taatattcag agcaaggtag aagaaagcta tgaagtaata
     2401 ttttctttat atataaagga aaatttaaat ggaaatatta gagtaggctt attgtataat
     2461 tagtatattt tagatattaa ggagagtgta tatgtctgaa aatataagag gtacagagta
     2521 ttggttaatg gaagaatttt tttatcagga aacagacaca ccacagattt ttagagaaga
     2581 aagcataatt gcacttggat ttgatataaa aataaattta aaaaaagtat tcagtatggg
     2641 gaaaaaatca gaaaagtata ttaatgagaa ttgtaatgat aaagatttta taaaaaagtt
     2701 tatgtcgatt aaaaaaggag attatttttt atataaagca tatcaaaata tagaagaaaa
     2761 agttaaatgt aatgtgtcta ttggcatagt tgaagaagat tttgaaaatg gatatgaatt
     2821 aagtcatatt ttagggcata cactaccagt aaaatggatg cataattttg atacagtttc
     2881 aaattgtaaa gtatatacaa gtgaatttta taaattaaat aataatgata ttagttcata
     2941 tttagaaatt attaagtcac aagataagaa attattgaga aaaagaatag aagaaagtga
     3001 aaataataaa tggtatttaa aaaactatta ttataaaaat gtagataagt gtgtagatga
     3061 tgaagaatta tttattcagc ccattaatct agaacaatta gaaagcctaa aaaatataaa
     3121 gacagatgat tatattttat tgtataatag cgagaaagta atttgtgaaa atgaccatac
     3181 aatgaagagt ctatttggcg tatgtcaagt tatggaaggg ttcgataaag agaaagtaag
     3241 tgctttggag ggtggatatt tttttcctgt tagatggata gagtatgaaa aggagaatat
     3301 agacggaaat gcatgggtta tatttgaaaa taatagtata gttgaaaagt atgtaaaaag
     3361 atttactaat aaaatgccat taaatactat actttatgga ccaccaggaa ctggtaaaac
     3421 atacaataca aaacagatgg tttacaatct agtaagtgaa accagtctaa gaaacagata
     3481 ctatacaggt tacttagatt ataaaaataa tgtagaattt tgtacatttc atcaatcata
     3541 tggatatgag gaatttatag agggccttaa gtctgatggt gaaggcaatt ttaagccaga
     3601 agatgggata ttaaaggata tatctataga agcatgttat aatgcattga aatttgaaaa
     3661 aaagatgagc tttgagttgg aaaaagatag cttgacttta gaaactataa aagacaggaa
     3721 aaagaaatta gtattagaaa atatagaata tagtgagagt tttaattttg aagaatcaga
     3781 aaactatgtt ttaataatag atgaaattaa tagagggaat gtatctaaaa tatttggtga
     3841 gctaataaca ttattggaag atgataaaag acttactaaa tcaaatcaaa ctattgtcaa
     3901 actaccatat tcaaaggaaa aattttctct acctccaaat ttgtatatag ttggaactat
     3961 gaacacatca gataaatcaa tagcactttt agatatagct ttaagaagaa ggtttaattt
     4021 tgaagaaatt atgccagatt atgatttatt gtctattgta gatggtatag atattagaaa
     4081 aatgctatat acaatcaata aaaggataga gtttttatat gatagagacc atgttattgg
     4141 acaagcttat ctacttgctg tagaaaatat taatgatata gtagtagtat ttagaagtaa
     4201 aataatacca cttcttcaag agtattttta ttatgattca gaaaaagtag gtttgatttt
     4261 aggaggaatt ggaagaaatg aaaaagataa atacattgtt tataaagaaa aacttatagc
     4321 ttctgagtta tttaaaaata ataatgcctc aataatggag agtaaagtta actattgtat
     4381 aaaacaaaac atatctagtg aggagctaaa aaatatatat gagtaaaaat atatatgtta
     4441 aggaaaccta tgaatggata aggataggta atggtgaaaa tgagcttaca gaaattgaat
     4501 atgaaaaatt gcttaagtat ctagagaaca ataatgatgt attaaaaagt aatataatag
     4561 atattaaata caaaaaactt agatttataa actatgtagg catcatatgt tttgaaaatg
     4621 taatacttga aatacttcct aaattgtcat taagtgataa tcttgtaaaa gatagagaga
     4681 tactattaca gatgctttct atatgtaata aaattccaat tacaatgaat gaaaaaataa
     4741 gattgagttt gaaaaattat aatctcttaa atttttttgt tatgtatttc attgagagta
     4801 tgcaaacaca gatgaaaaaa ggtatttatt ttgaatatat aaataaaata gagaacttaa
     4861 atgttatgag gggtaaaata ttattaagta catatgcaaa agaaaaaggc atttctccaa
     4921 tgaaaataag atgtgaatat gatgagtata gtgaaaataa tttcttgaat caagttctta
     4981 aaaaagcatg tatatctatt ttatgtagaa taaatgacaa ttcaattcaa ggcaagatta
     5041 aaaaaatttt aagctatttt caaaatgtag atttaattta tatagataga aaaaaactgt
     5101 tggattataa attttataaa aacaatgata gatttaaaga ttgttattta ctagctagac
     5161 ttctactttt aaatttgtct atggataata gtcaaaataa tcaagaggca ttttctatat
     5221 tatttgaaat aaatactttg tatgaagaat atataggaat tttaatcaaa agtatatggg
     5281 acaattcatt tagagaaaca tatatacaag ataagagtaa atttcttttg aaaaatgaac
     5341 aaactggtaa gaaaaacttt aatttaagac cggatatagt tttatatgac ttaaaaaatg
     5401 aatatgaaat tataattgac actaaatgga aagctattga agtagattca aatgtttttt
     5461 ataggtcaag tgatatatac cagatgtatg catatataac agcatatgaa aatgcaaaaa
     5521 gatgtatatt attatatcct tgtattcaaa aagataagaa ttatagttcc tggaaattat
     5581 cagaatcatt taagggtaag tttatagaag caaaaactgt gaggttagat gatataaaaa
     5641 acacaaaaaa tgattt
//
LOCUS       DAMJYX010000164         5644 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJYX010000164 DAMJYX010000000
VERSION     DAMJYX010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.169"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            281..1552
                     /gene="serS"
                     /locus_tag="PC479_003352"
     CDS             281..1552
                     /gene="serS"
                     /locus_tag="PC479_003352"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2805868.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            1830..3335
                     /locus_tag="PC479_003353"
     CDS             1830..3335
                     /locus_tag="PC479_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2805869.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            3449..4147
                     /locus_tag="PC479_003354"
     CDS             3449..4147
                     /locus_tag="PC479_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2805870.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            4432..5520
                     /locus_tag="PC479_003355"
     CDS             4432..5520
                     /locus_tag="PC479_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805871.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT         2282 a          558 c          941 g         1863 t
ORIGIN      
        1 atacaagatt gataaagaaa aagtcaaaat agataattga ttagcaaaat agatataatt
       61 ataatgaagt taaatctagc aatattctcc taaaattaaa ttacatttat ttaattaaat
      121 ataatttgac aatggacaaa aaagtgagta aaatataaat aaaataaaaa taacaggttc
      181 ggataaagaa aaaggtttct taacctcaag cataaagtat attatctaag ttatttgaga
      241 ttaagagata aatccaatct taggtactga ggaggaaaaa atgcttgata taaaaagaat
      301 aagagaaaat ttagattgta taaaaaaagc aatggaaaga aggggcgaaa aagactttaa
      361 ccttgatgaa gtagttaagt tagatgatga aagaagaaag attcttcaag aagttgaagt
      421 tatgaaaaat gaattaaata cagcatctaa aaatatacca aatcttataa aagaaggtaa
      481 agatgtagaa aatgaaaaga ttaaactaaa agagttatct gataaaatta aggtaataga
      541 ccaaaatctt aaagaagtag aagataagat ggaataccta ttaatgagaa taccaaatgt
      601 accacatcca gaagtaccac aaggtgaaac tgatgaagat aatgttgaag taagaacttg
      661 gggaaagact actacctttg attttgaatc taaggctcat tgggaaattg gaacagaatt
      721 aggtatactt gattttgaaa cagctagtaa aataactggt tcaagattta ctctttacaa
      781 aggattaggt gctagattag agagagcatt attaaatttt tacttagaca cgaatacaaa
      841 agtaaatgga tatacagaag ttattccacc atttatggca aatagaaata gttttttagg
      901 tacaggacag ttacctaagt ttgaagaaga tatgtttaaa atagaaggta tggactattt
      961 tatgattcca acatcagaag ttcctttaac taatatacat gctaatgaaa tacttaaatt
     1021 tgaacaatta ccgataaatt atacagctta tactccatgt tttaggtcag aagctggttc
     1081 tgctggaaga gatacaagag gtttagtaag acaacatcag tttaacaaag tagaaatggt
     1141 taaaatagtt gcaccagaag aatcttacaa tgagttagaa aagcttacta acaatgcgga
     1201 aactatgcta caattattga atctgcctta tagggtagta aagatatgta caggtgattt
     1261 aggatttacg gcttctttta aatatgatgt agaagtttgg atgccaagtt acaataggta
     1321 tgttgagatt tcatcttgtt caaactgcga agatttccaa gctagaagag caggaataag
     1381 atttaaaaga gataaagatt ctaaagctga atatgtacac acattaaatg gctctgggtt
     1441 agctattgga agaagtgttg cagctatatt agaaaattat caacaagaag atggttctgt
     1501 ggttgttcct gaagttttaa gaccatatat gggagtttct gttattaagt aagagttaaa
     1561 ttatgtattt ttaagcaata caataatata gaggtaataa aaaataatta atataaaagt
     1621 gatacaataa tatagaagta ataaaaaaat agttaatata aaagtgatac aataatatag
     1681 aagtaataaa aaaaaataat taatataaaa atgtgtacaa actaatgaat attgatatag
     1741 taagctatca tatgaaagag tatgtacaca ttttttttac taatttttca caatatgaac
     1801 ataaataatt tacaggaaag gaatctttta tgaaaattca aaatactgag tggggatata
     1861 ttgaatggaa acatacttat gatgaaaata atccaaaaca agctatgaat atatacattg
     1921 cagtaactat gcctggtaag aaacatttta atcatgttca ttatggtcaa gagcagatga
     1981 tatatatttt agagggagag ggtttgtata ttataaatgg agtttggaag ccattttatc
     2041 aaggtatgat tttttatata gaatctggta gcacacatga aactataaat acaggtgata
     2101 gagaaataaa agaacttatt gtttcaaata atgtagatga tgtaggagag agtgaagtta
     2161 tagatattaa tcctaataat tatttaaaga agaccttgat aaattatagt gaaagtacat
     2221 taaatttgta tgcagcagta gaatcaattc gtggtcaatt tatagacccg tttaaaattc
     2281 cattaattat atatgatgat tcatggaata tagttttaaa aaatccttat tttccacttt
     2341 tttgttttga aaaatgtaat cctatgaaat ttcctcaaaa ttgtgattgt atgaatcaaa
     2401 aaagtagcaa tcaatttgta tgtgaatatg ggataactat atataatata ccaatactgt
     2461 acaaaagcaa tagcataggt gttataagag gagggtatgt actattatct gatttgaatt
     2521 tagatacaga gcataataat ttatatgata ttccagaggg agctgctaga agtataaaac
     2581 gcctcttgaa acaaatatct aaaaatataa taaatttttg ctcttttaat gatataagaa
     2641 aggatttaca agaaaaagag aagactatag caagaacata tcattatgga gagcaacttg
     2701 aaatgaattt aaaagtagct caagatatgg ttactaattt aagaataaat catcactttt
     2761 tatttaatac attaaattca atggcaagca tagcactaga tgatggtagt tatgatttgt
     2821 atagtgcaat aatagattta tcaagaatgt ttagatatac aatgaggtct gatttaagat
     2881 ttgtagaatt agaatctgaa atattataca ttaaaaatta tttgaattta cagaaattac
     2941 gttatggaga tgcacttaaa gtaaaatatc ttattccaga gaagttatat aacctttctg
     3001 tgccttttaa ctttattcaa cctattgtag agaatgcatt tacacatgga tttagagata
     3061 ttgacacaga aaagcgaatt gaaataattg caagattaga ttctcagtat gctattatag
     3121 agattcataa taatggaact atcttagatg gaaataatat agacaaaata aaggcaggta
     3181 ttcgtagtaa taatggtcat ggtttgtctt taatatatac aaaattcaca tctgcttatg
     3241 gaaataattt tgatatggat ataaagtcga gtgataatga aggaacttac ataatgatta
     3301 aaattcctat tgaaaattat aaggagtatg tatagaactt gtgaaaaatt aataagtttt
     3361 ataaatgtta agaaatatat tagatgtttt tatgttgttt tatatatctt acttttttat
     3421 attattaagc taaaagggag gtattctcat gagaagagca ctaataatag atgatgaaga
     3481 agctgttcta aaaataataa cacattttat agaaatgaaa gatatgccaa taaaaattgt
     3541 tggtaaagca acatctggtg atgaagcagt agataaaatc attggtttag agccagacat
     3601 agtttttata gatattcaaa tgccaatata caacgggtta gaagtaatag aaaaaacatc
     3661 tcatttatct aaaaaaataa actttgtagt aataactgca tttaactatt ttgaatatgc
     3721 tcaaaaagct ttgagattaa atgtaaaaga tatactcctt aaaccattgg atatgaaaga
     3781 atttactaag tctgtagaaa aaattattgg atatcaatat accaataatg atttattaaa
     3841 tgagatactg gaatatataa atctaaacta tagcaatgac ttaaagttaa atgattgtgc
     3901 tagattattt ttaaccaatc catctaatat atctaggatt tttaaaagta atttaaatac
     3961 aacttttata tcatatctaa atcatataag aatagaaaaa tctattgagt tacttgaaaa
     4021 tactacaaaa agtataaatg agattgcaga aatagttgga tataatagcc taaataactt
     4081 ttataaaaac tttaagatag aaaaaggaat gactccaaaa gtttataaac tgaacactaa
     4141 aaattgaaat tgataaataa aaagtcaata tagataattg tttgagtgtg tctgaataag
     4201 tatactttaa ttatgataaa aattaaagcg ctttaaacat atttaataat attaaacctt
     4261 ataaaagctg tacctattta tataaaaaaa tatatataaa taaaaagaaa aagatataca
     4321 aaataaaaaa agtgaattaa atactgtttc aaaaagacag aattttcatt taatttactg
     4381 agagtttata tttcttaaag gaaggtaact ttataatgga ggtaatttaa tatgtcagac
     4441 aaagtgaata tgaaaaatat tatctgtatt gcaggcgctt atctagcaac agcaattggt
     4501 tctgggtttg ctactggtca agaaattcta caattttttg cctcacaagg gtatatgggt
     4561 ttaattggag cattaatatc aatggtattg ttttcatttc ttggtgctga agttataact
     4621 agaggtagag aattaaaact taaagaacca acaagaattt acacttttta ttgtgggaag
     4681 attttaggaa aattttacga atggtttagt ccaatatttt tatttgggat tcttatagta
     4741 atgatttcag gtgcaggctc aactttaact gaatactatg gtataaatcc ttacttagga
     4801 agagtattga tggctgttgc tactttaatt acagtgtcta tgggtttaga aaaattagta
     4861 agcatacttg gacgtattgg accagtgata gcagtattta caattgctgt tggattaata
     4921 agcttgttta agcattttga tggtttatct atggttggag aagctatgaa aaatattgaa
     4981 gttacaaaag catcagaaag tccaatcgtt tctggtgtaa tatataatac attaaatatt
     5041 attgtaatga ttgttttttt aacaggaatg ggagcttcta ttgaaagtaa aaaggatgct
     5101 ttttggggag gtttattagg tggaatagtg tttatgactg ctggtatagt tatgtatttg
     5161 gctatgattt cagatatagg aaatttgtat atgaaagaaa taccatcact ttatttagca
     5221 gataatatat ctcctattgt aggtgtatgt ttttcagttg cattaatatt aggtatctac
     5281 acaacagctg ttccactttt atggtctgta accaatagaa ttgttgaaga tgaacatcct
     5341 aaatttaaat tggtaacaac agtaatagct attttggctt gtataggagg ttttttaccg
     5401 tttggtaaac tagtaaacat tttatatcca tatacaggtt atatggggct tttaatttta
     5461 gtctgtatgg tgtataaagc aattacatgt agaaaaaaat caacacaaag tcaggaatag
     5521 agccacttat taatagtttt tatttaaaga aattttttat ataatccgaa tttaaaaagt
     5581 caaaatagtt aaataaattg taaaatatga taatatattt aattaaaata atatagtaaa
     5641 atat
//
LOCUS       DAMJYX010000165         5634 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJYX010000165 DAMJYX010000000
VERSION     DAMJYX010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.170"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(98..877)
                     /locus_tag="PC479_003356"
     CDS             complement(98..877)
                     /locus_tag="PC479_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2805872.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(932..1414)
                     /locus_tag="PC479_003357"
     CDS             complement(932..1414)
                     /locus_tag="PC479_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2805873.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(1680..3101)
                     /locus_tag="PC479_003358"
     CDS             complement(1680..3101)
                     /locus_tag="PC479_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2805874.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(3247..4017)
                     /locus_tag="PC479_003359"
                     /pseudo
     CDS             complement(3247..4017)
                     /locus_tag="PC479_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(4022..5224)
                     /locus_tag="PC479_003360"
     CDS             complement(4022..5224)
                     /locus_tag="PC479_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2805875.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         1844 a          987 c          555 g         2248 t
ORIGIN      
        1 catactaatc aatatttttt gtgtatttta tagattaaag attaatgatg tctgtaattt
       61 aaacttacaa tcaccactaa tcttcaaaca aatattttta cttttgcact tgcttatttt
      121 tatctaaatg ttgtataagt tcagatactg ttacgaattt atatcctctc tcttttaatt
      181 taggaattac tacttctaaa gcttgtatag tttgtgattt atcatttctt ttagtgttat
      241 aatcatgtaa aagtattata ttaccatttt ggacctttgt aaggatagta tttataattg
      301 catctactcc agggttagac caatctctag ggtctaagtt tgaccaaaga attatattca
      361 ttcctttaga aactataata tcacagacac ttttactcat ggctctatat ggaggtctaa
      421 atatctttgg ttctactcct atcacacttt ttataatatt ttgcgtatct gttatttctt
      481 tttcaacact actataacta ttttttgcaa catttatatg tgtaaatgta tgattcccta
      541 tttcatgccc ctcatcatat tgtcttttta ataattcttt gttatattct actttttctc
      601 caactacaaa aaaagttgct tttacatcat ttttctttaa tatatcaagt acttgtggtg
      661 taaaatcctc atctggacca tcatcaaaag tcaatgctaa aattttttca tcctcatttc
      721 ctttttttat tattacatct tcataatcgt cttttatatt gtcatttaaa ctatatgctt
      781 cttttgaact atcttgatta tgtgtattat gattatatgt atatacacct actatagata
      841 ataaacatat acatatacca catattattt tcttcatgtc attacctctt ttctaattca
      901 ttctagcaat atattttata atgtcagttt tttaatctct caatattcca tttttattta
      961 aatcagttaa atttgttagg tcttctgaat aaaagttaga atcttctaca tcagggtttt
     1021 gcattgcatc acctaataaa ttattttcct tctttgcaca atcattacat aaacttgtat
     1081 ctggcaatat atccaatctt tctaaatcta tttgattatg acataaatca caaatcccat
     1141 atgttccatt ctctatttta gataatgcta agtctatttg atacaatctt ccctcttcat
     1201 gatttattag actgttttgc atattttgca tatataaatc tgttcccatg tctcctatat
     1261 gattatcata actagataat tctcctgatg aatacttttc acttgtatgt ttagttgtat
     1321 ctccaaataa agtattatct ttcatatcct ctactaaatt agttaaattt tctctttctt
     1381 tcaataattt atctttatat tttttagtat ccatttttaa cacctctaat tttaatattt
     1441 acatcataaa aatagtatgt ggaaaagtaa attaaacata caataaaaat aagatttgat
     1501 tcgaaaaatg gaatgtctca aaatgaaatt tttagttcat ttaaccctct tttttatatg
     1561 aaatcaaata tatttttatc ctttcagcaa taaaaaagag acttatctct attttgagat
     1621 agtctcttct ttgaacataa tttaaataca attttttaat ttttatagtt attattttac
     1681 taacttgcca atttatgatt attttgttct ttaatatgtt ttgctttttt aaagaataaa
     1741 agaccaagaa ttattgctac aataacacct attccattag atatattaac tggtagtcta
     1801 aatccttctt ttgcttgcat tatataacta aatgttacta cagtcataaa aagtgctggt
     1861 aatgtagtta tataatggtt tttatttgct ttgactaaat atgcagaacc agtccacaac
     1921 attatcattg caagtgtttg atttgcccaa gagaaatatc tccaaattat attaaaatct
     1981 ataaatgtaa gagcaacacc aactgcgaat aatggtaaag ctattaaaaa tctatttttg
     2041 actgcatctt gattataatt cattgcatct gctattgtca gcctagcact tctaaatgct
     2101 gtatctccag aagttatagg acatgccaca actccaagta tagctaaagc tcctccaata
     2161 ggccccatta atccaacaga tactgtattt actactgttg ctgctccacc atcattaaat
     2221 atcacattaa gttgaggtat gccatggaaa tatgacatgg ctgctgctgc ccaaactaaa
     2281 gctattatac cttctgcaac cattgaccca taaaatactg gtcttgcctc actctctttc
     2341 tcaacacatc tcgccataag tggtgattga gttgcatgaa aacctgatat tgctccacat
     2401 gctattgtta taaataagta tgggaataaa ggtgttccat ttggattcat attttgtaaa
     2461 tttatatttg gtatgttata cccctttatt attaatcctc cagctacacc tactgccata
     2521 attaataatg ctgctccaaa taaaggatat attttaccaa tcaatttatc tattggtaaa
     2581 acagtagcca taatataata aagtattatc actccaaccc atatttgtgc gtttacacct
     2641 gtcaatgtct ttaataatcc tgctggacct gttacaaata caactccaac caatactaaa
     2701 agaataacac taaatattct cattgctatt tttgcaccat ttcctagata aaggcctacc
     2761 aactctgatg cagatgtacc tttgtttttc attgatagat atcctgaagc aaaatcgtgt
     2821 actccaccag caaagattgt tccaaacaca atccataaaa atgctgatgg tccaaataat
     2881 gctccttgta tagctccaaa tatcggacct aaccctgcta tatttagaaa ctgaattaaa
     2941 aagattctct tccatttcat tggcacataa tctactccat ccctacaagc tatagctgga
     3001 gtttgatttt tgtcattggt tccaaaaacc ttttctacaa aagtaccata gacaaaataa
     3061 cctactataa gtactactat tgaacctaaa aagcttacca taaattcatc cccttagttt
     3121 ttatataatt gctttataaa tttttatgag tttaagaaaa ctatttccag ttcaaatagt
     3181 aatacttttt tatgctaata taaatattaa ttacattaag tgcatattta agtacatgaa
     3241 atgactttat atatgcatta acagcctaaa ttcttttact ttgcttctac ttacagttac
     3301 ttcataatca ctatttttta attttaatat gtatgtatta ttaaaccatg gtataatttc
     3361 ttttactttg gttaagttta cgatataaga cctatgtgtt ctaaaaaatg ttttatcatt
     3421 tataagattt tcgacttctt atatgccttc ttttaatata aacttttctt tctcagtata
     3481 tataaatgtc tgcctttcat tagcttcaca gtaatatata tcatcaatat ctattacaac
     3541 taatttatca cctttccaaa ggctaatttt atgtgttatt tcttctatct cctgatttac
     3601 tgcttccttc ttatatttgt ataaattact atttacatta aatagttcaa tatcactcat
     3661 ctcacttttt tctaatttat tcagcataga aataattctc tcttcagaat atggtttcaa
     3721 tatataatca aatacatcca aagaaaaagc atctactgca taactttcat atgctgttat
     3781 aaaaacaatt ttaggtcttg atttaaactg atttaatgtt tttgcaagta gcattccatc
     3841 aagatgaggt atatttatat ctaaaaaaat tacatctatt ttattttcct gaataaagtc
     3901 taacacctct ataccattgg taaattcact tactacctcg attccacttt tattttcaat
     3961 aaaatatctc aattcctttc ttgctggaaa ttcatcttcc actattatag ctctcatact
     4021 tctactcctt tacataaaat actatcaaag tcccattatc tattcttctt atattaagcc
     4081 ctcttccata catcagttta agccttaaat gtacattata aagcccaatc ttattttctg
     4141 gcatattatc ttgatataca ttatttataa tttcctcact tatacctata ccattatcct
     4201 ctattgatat tctcacagta ttttctaatg aatacggtaa cttttttatg gatattttca
     4261 ctacaccagc tctaccactt tctaaaatac catgtattat tgcattttcc actaatggtt
     4321 gtataatcaa acttggaatc tttatattta catcttctat atcataagat acagttagta
     4381 attctccaaa cctagctttt tctatctcta caaaagattt aacttgttca atctctttat
     4441 ttatatcaat taagttatca ctatattcaa gattatatct tagataagtt gataaattta
     4501 ttattaactc tctagcctta tctggactaa atcttataaa tgaagctatt gtgtttaggg
     4561 aattaaacaa aaaatgtgga ttaatttgtc tttgaagtgc ttttatttct gctttatttc
     4621 tcgcttctat atctttttta tctttctcaa tcccctctac tatagatata acaaatccta
     4681 ttccaatctg cccaactatt aaaggtaaat atattccacc gattatttga attgctaaag
     4741 aatgtggata tgatattaag tatataagta atatagtaaa actttcagaa atcatgccta
     4801 ccaatattcc atacattact ctatgttgtt ttggtatcct cttatataga aatcctgata
     4861 atacacctgc tagtatactt gataaaagac atggaacaga tgttatcccc ccaatatcca
     4921 taaaatatct atgaatccct gaaaaaactc ctgctgtaat acctaccatc ggaccaaata
     4981 aaatgccacc tgatactata gatattattc tagtgtttac tattgacccc atataattta
     5041 taccactata agttccaaat atggctaaaa aagtaaaaac taatgctata cagaccaagt
     5101 cattaaaact atattcttcc ttttgaaata tctgcttaaa tatttttaac tttgttatta
     5161 gaagaaacaa tactacaata aggcttgtct tttctattaa tgcgattaaa attgatgttt
     5221 tcataatccc cctttaaaaa ataaattttt acacactcta aatttattat ggttgttata
     5281 aattacatat ttgaagtaat cctatcacaa aactttccac atttaatctt ttaattaaat
     5341 aagaaatagt tgtacttcta ttaacaacta atactagcta gcataagctt gtaataactt
     5401 gtagttatta ttaactttac aattctagta tttaatttta tactttagaa atctcttttc
     5461 ttagaaataa tactttcgaa tatcatataa tacaatatta tgtagattct tactcataca
     5521 acatatttct ttaaaaattt tatactattc tttgttcaaa tatctcattt gatttaattg
     5581 tgttaaacac acattatcat ctctctgtta tatatttatt atatagccgt atat
//
LOCUS       DAMJYX010000166         5579 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJYX010000166 DAMJYX010000000
VERSION     DAMJYX010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.171"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            296..1786
                     /locus_tag="PC479_003361"
     CDS             296..1786
                     /locus_tag="PC479_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2805876.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            2066..3562
                     /locus_tag="PC479_003362"
     CDS             2066..3562
                     /locus_tag="PC479_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2805877.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3696..4622
                     /locus_tag="PC479_003363"
     CDS             3696..4622
                     /locus_tag="PC479_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2805878.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4740..4931
                     /locus_tag="PC479_003364"
     CDS             4740..4931
                     /locus_tag="PC479_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805879.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            5154..5504
                     /locus_tag="PC479_003365"
     CDS             5154..5504
                     /locus_tag="PC479_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805880.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2246 a          546 c         1050 g         1737 t
ORIGIN      
        1 cgaaaatgta aatgtagata ataaatttaa tatcaaaaaa tgtaacaaaa aggtatgtaa
       61 gtaaaaagta aaattaaaat atataataaa aaatattata tattagattg aaaagtaaaa
      121 ataaaaatag tgaaatttca gaaatatcaa taatattata taataaaaca aaaaaacact
      181 atatatattc gataaaataa aatttaaaca tataatattt tatgtatata ttaataaaag
      241 gacatattgt gttataatgg tatcgtataa aatcatttac gggggtggta ttaaaatgtt
      301 tcaatttgaa acacaaatac acaaattaag acatgatgtt ttaactgcaa tagctagact
      361 tggaaaagaa gataacctta ccagagaagc aatggataat attcagtatg aaataataaa
      421 aggtgagaaa cctacataca gatgttgtgt ttataaagaa agagctataa tatctgagag
      481 agtaaatgta agtatggggt tacaaccagg tcaagataag atagatttag atacaatgga
      541 tatagaagaa gatgaacaaa ttatatatat tttggaagct gcatgtgaca gttgtccaat
      601 aaatgaattt acagtaacag atatttgtag aggatgtcta gcacatagat gtaaagaagc
      661 ttgtaaattt ggagctataa cacatgtcgg aggaatggcc tatataaatc atgaattatg
      721 taaggcttgt ggaatgtgta aaaaagcttg tcaatatgat gccatctcag aggttgttag
      781 accatgtaag agtgtttgtc caactaatgc cttaggattt gatagagaaa atatgaaagc
      841 tatgatacat gaggataaat gtttaaactg tggagcatgt atgtcagcat gtccatttgg
      901 agcaatatct gataagagtt tgatagctcc tgtagctaga aagttagttc aaaaagagaa
      961 aatgtatgcg gtggtagctc cagctataac aggtcaagtt gaagcaaatg ttacatatgg
     1021 tcaaataaaa aatgctataa aatcactagg atttgttgat gtattagaag cagcttgtgg
     1081 agcagatgca gttacagttc atgaaagctt agaatttgct gaaagaatgg aaaagggaga
     1141 agcttttatg acaaattctt gttgtccagg atttgttggt tatatagaaa aaacataccc
     1201 aaatcatgta gggaaaatat cttctacagt atctcctatg atagctagtg gtagatttat
     1261 aaaaagtcat gaaccagatg ctaaagttgt attcatagga ccatgtacag ccaaaaagag
     1321 tgaggcagct aagaaagaat taaaaggagc tgttgattat gtacttactt ttgaagaaat
     1381 aatggcatta tttgaggcat ttgatataga cttggctaag tgttcaaatg aagatataga
     1441 tgatgcatca atttttggaa gaggttttgg agctgctggt ggtcttacaa aagctataga
     1501 aaattatctt gctgaaaaag gtattggtgt taattttgaa cctgtaaaga tttctggttc
     1561 aagagaaatc aagaaaacta tgactatggc taatttagga aagctagaag gtaattttat
     1621 agaaggaatg atgtgtgaag gtggttgtat aaatggttct ggtaaattct tatcaggtgc
     1681 aaaggttaaa gccacttttg atagaaaaaa ttctcaatca actaagaaaa gtgtcttagc
     1741 aaatacaaag ataaaagatt ttagtaatat aaatttagaa aaataaagat aatacaaagc
     1801 aacaaaaata catattgtta atagagttgt tctaagttga ggtttaactt ttggacaact
     1861 ctatttttat ttatatattt aattattgaa caactaaaat aaaaattata tacacttaaa
     1921 ataaaaatta catatataat attttgtata taacttaaca aagtatatgc ctatgttata
     1981 ataatttata gaaataaata aaaaatacaa tatacataat agtagaaata tagcaaaaag
     2041 gtgtgattta tagggaggat tacttatgtt tgaatttgaa acgcaactaa aaaggttaaa
     2101 acatgaagtt ttaacagcag tagcaagact atctattgaa gaaaatttaa caaaagaaaa
     2161 tatagaaaaa attccatatg aaataattaa aggagaaaag gcgagatata gatgttgtgt
     2221 ttataaagag agagaagtag ttcttgaaag ggcgcaactt gcattaagtt taactccaaa
     2281 tagtaaatat ggagatataa atccagattc agttagtttg gacacagatg agcaaattat
     2341 atatatagtt gaagcagcat gtgatagatg tccaataaat gaatttacag ttacagagct
     2401 ttgtagaggg tgtttggctc atagatgtaa ggaagcatgt aaatttgggg caatttctta
     2461 tataaatggg agagcatata taaatcatga tttatgtaag gcttgtggaa tgtgcaaaag
     2521 ttcatgtcaa tatgatgcta tatcagaagt tgtaagacct tgtaaaagcg tttgtccaac
     2581 aggagctttg gattttaata gaaacactat gaaagcaatg atacatgaag ataattgtat
     2641 aaattgtgga gcatgtatgt cagcatgtcc ttttggagca atatctgata agagtttgat
     2701 agcacctgtt gcaaaaaaac ttgcaaagaa agaaaatatg tatgcaatag tagctccagc
     2761 tataacaggt caaatagata gtaatattac atatggtcag ttaaaaaatg ctataaaatc
     2821 actgggattt gtagatatga tagaagcttc ttgtggagca gatgcagtta cagttcatga
     2881 aagcaatgaa cttattgaaa gaataaaatc aggagataat tacatgacaa attcatgttg
     2941 tccgggattt tttaagtata tagagaagat gttcccagat gaaaaagata gaatatcatc
     3001 tacagtgtct cctatggtag caacaggaag attagtaaaa aaaatagatt ctgatgctaa
     3061 agttgtattt ataggtccat gtacagctaa aaagagtgag gcaacagaga aaagtttaaa
     3121 agattcagta gattatgtac ttacttttga agaactaatg gcattatttg aagcatttaa
     3181 tatagattta tcaaaatgtt caaatgaaga tataaatgat ggttcgattt ttggaagggg
     3241 atttggagca tctggtggac ttgccaaatc agtacagaac tatattgctg aaaaggggct
     3301 tagttttgat tttgaaccta taaaagtatc tggtccaaga caaattaaaa agacaatgac
     3361 aatggctaaa ttgggaagat tagaaggtaa ttttatagaa ggaatgatgt gtgaagaagg
     3421 ttgtattaat ggagccggta agtttttatc aggtgtaaaa gccaaaaata cttttgatag
     3481 aaaaaatgga caatctacta aaaagagtgt tttatctaat gataagatta aagaatttga
     3541 agatgtaaat ttagaaagat aattgttttg gtacagtcat ttttttatta tgccacaaat
     3601 atgcctaaaa tttgcccttt ttatatgtgt attttacaga gttaaggttg ctatactata
     3661 aatatgtaat aaataaaatt tataggagct tttggatgag gaaaatagta ttaaaagata
     3721 tcctgttttt agttactaca ttatctataa tagtaatatt attgcttgta acaaatataa
     3781 ctactaatag taagataaaa tcaacaagtg aatcttattt aaaaagtgac aaaaatagtg
     3841 tgaggcaaaa tggtatccag aggagcacaa agagtagcac aaaaaatact gcatttaaga
     3901 ctactatact aaacactgga aagtctgatt gcatattggt tgagataggt aacaaagtca
     3961 taatgattga tacaggagaa gataaaaatg gaaaacaaat agtggatagg ttaaaagaaa
     4021 aaggaataaa tacacttgac tatttgattt taactcattt agataaggac catataggtg
     4081 gagtagatag tgtgttatct agtgttaaag taaagaatat tatacaagca aattataaga
     4141 aagatagtaa gcaatatgat gaatatattg actcacttaa aaaagctgat atagaaccag
     4201 tgctactaaa agataatatg aatattgtaa tcaatggtgc agaaataaac atacatccgg
     4261 cttcaaaaag caagtacgag agttcaaatg attattcaat aatcactaat ataagctatg
     4321 gagcacataa gtttttattt gcaggagatg cagaggaaga aagattgtca gagtttataa
     4381 atggaaatac tttaaaatat gattttgtta aaatgcctca tcatggaaga tatgacaaat
     4441 taactgaaac atttttagag tctatttcac ctcaatatgc tgttattaca tgttcagaga
     4501 aaaaagaacc agaggaaggt gttttaaaaa tattggaaag attgaatata aaaacatttt
     4561 tgacttctaa tggagaggta gttatcaata gtgatggtaa gacattgagt gtgaatcaat
     4621 aattttttta aaaatatcta aaaaaattta aaaaaatcat tagcaaatat taccccttaa
     4681 acatagtata ttttaggagc tgaaactcca catacaaaat gactaggagg taacatatta
     4741 tgttggatag attttttggt gaaggcggat ttggtggatg tgcttggtgg ataataatat
     4801 tattcttctt attcttagca ttccaagatt gttgggctga aatggactta tgtgcatgga
     4861 tacctttctt aatattatta ttaatacttt gtgaatgtgg tgacgttttt gatggaggat
     4921 gtggatgcta aaaaaatcaa tttagatgat ttaataaagc ttatggtggg tataaataaa
     4981 ataccaaacc aatcacaatc tagtgtttga aggtagtcgg aaatcccccg ctaccttctt
     5041 ttttttattg ttaaaattta tttttgaatt atttcattta catataataa tttttacgat
     5101 aaagtctgat ataattaaaa agaaaataaa atttatttat aggagatgta aaaatggcta
     5161 tagtggataa gattttcaat gagataaaac ttatggttgc aagactttta ttgggaaaaa
     5221 aatacagtga atatgaagaa aaagatttag tgtataatac agaagaagaa gttatactta
     5281 taactttaaa aagattagtt tttcagggaa atgtaaacga agcagaagaa attttatttg
     5341 ataaagctaa gagtgtaaat agtgaaaata tgcagtatat agcaatagaa ttttatacta
     5401 tgcttatgga aaagactgat gaagaattag aagctatgaa tttttctaaa caagaagttt
     5461 atcaaggtat agaagatatt agaaaagtat tgaatttaga ataaaagata tatgaaaata
     5521 ttgaaataaa ttaatgctaa ggtgagaata ttatacttat atataaaaat atagtattc
//
LOCUS       DAMJYX010000167         5573 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJYX010000167 DAMJYX010000000
VERSION     DAMJYX010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.172"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(202..885)
                     /locus_tag="PC479_003366"
     CDS             complement(202..885)
                     /locus_tag="PC479_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2805881.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1166..1756)
                     /gene="zapA"
                     /locus_tag="PC479_003367"
     CDS             complement(1166..1756)
                     /gene="zapA"
                     /locus_tag="PC479_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2805882.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1781..4174)
                     /locus_tag="PC479_003368"
     CDS             complement(1781..4174)
                     /locus_tag="PC479_003368"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2805883.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4191..5210)
                     /gene="pheS"
                     /locus_tag="PC479_003369"
     CDS             complement(4191..5210)
                     /gene="pheS"
                     /locus_tag="PC479_003369"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2805884.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5260..5494)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1697 a         1087 c          602 g         2187 t
ORIGIN      
        1 aatacttcta ttaatctata tattgcctac tatacttaag acattttaat taatttatac
       61 actgcttact atacttaaaa tacttctatt aacttataca ttgcttacta tattttaaat
      121 tctttaatta atctatacaa tatttgctat atataaagta tatcaaataa cagcttcatt
      181 tttttgtaaa tttacagaaa gttaattttc attaaatgaa tcatctttat tgataaacat
      241 aattccaaag accactgcca aaataaaaaa acatattagt attgattttt ccacgaaaat
      301 ttttgtaaaa aaagttccta aaatagctcc agatataaag aatataataa tagcataata
      361 ctgtaaactc ttttttatca taaaaatatt cttagtatgt atataatgaa ataaagtttc
      421 tgtagcactt cttaaatttc cagtacacat tgtcgttgca aaactattcc catttacttt
      481 tctaaaactc tctacttgca ttgaacaaat aaaagaaata gcaacattta caaacatatt
      541 aaatcttcct ctaggaataa atgaaacaat aagtattgta atcatttcaa ttataatgat
      601 aacctgacgc caatgaatct ttgtcttatt atttgtaaaa tactttctta tcttctcggc
      661 tataaataca cctaacataa atgccaaaat aggaatcaaa taataaaatg attcctttac
      721 attaccttct gctatattaa gcccaaataa aactatattt ccagtttgtg cattagcaaa
      781 gactttacct cttaccacat aagtataaat atccaaaaat cctcctgtta ttgccaacaa
      841 agaacataaa aagaatgatt cagacatctg actcttgctt gtcatatata aacctctcgc
      901 tttctaatga tagatacaaa atcaaaatca ttttagattc tatttttcat tgatataaaa
      961 aaattattta tatttaaata ttgtctatat ttatacatta tttgtattta tatagcattc
     1021 atatttattt atatttaaac attatctata tctatatagc atttatattt atttacattt
     1081 aaacattatc tatatttata tagcatttat atttatacat taaaagaagc tgtctcaaaa
     1141 aacaagagac agcaaatctt ttagtttatt ccacattttt tgcatcatta tttaaccctg
     1201 tacattttaa ctgtaaatca taagctttgt tttgaaactc agaagctaag ttttcagcta
     1261 tagtagctct ttcatttgct tcttcaagtt gagtacttaa ctcctcaatc ttgtttttat
     1321 aagcatctaa ctctgctcta gaaccttctg tcgtattact aagttcgaat atttcttgct
     1381 ttttattttc tataatctca attaactctt tcatcttagt ttcagcttca cgattttctg
     1441 cttctttttc tgctatcgta agctttaaag atttgatttc taattgaagc tcttcattag
     1501 atgctccaac cttcttactc aattcttcat tcgctttaat caacttctca ttctcatctg
     1561 aacattcaaa taaaaggtct gatatattta atgcagttaa tatcgcagca actgataagc
     1621 tcaatttcgg gttttgacgt gttattttat ccatttcttt gtctacaaaa tcagcaatac
     1681 taatcataaa cttctctgat ttgtctccaa ccattggata ttcagcgcca tggattttaa
     1741 ccattacttt gttcatccta acctcccccg acattagttg ttaatttgat cttaagttag
     1801 catttaattt ttcgcttaat tcacttaata tcttatcatg caccttagct acatcttcat
     1861 ctgttagagt tttatcttta cttctataag ttattgaata tgcaattgac ttgtatccag
     1921 cttctatttg agaacctttg taaacatcaa ataacttgta gctttctact aatccttgac
     1981 cattttcctt tattatatct tctatttgtt ttacaaatac atcatcttta acaataagag
     2041 ctatatctct tgatgtagat ggatattttg gtaatggcac ataattcttt gttctatcag
     2101 aattatcaaa tacaaagtca atatctattt cagcgacata aactctttga cctagattgt
     2161 agttttctat aacatctgga tgcaattcac ctaatgtacc tacatattta ttattataca
     2221 ctattttagc acatctacca gggtggaagg tagtattatt atcttgaggt tcaatttcat
     2281 aacctttaaa tccgacattt acaagtatag tttctatagt tccttttagt gcaaagaagt
     2341 caacatcttt accatacata cctacacaca ttctattttc ttccttaggt aattctgaat
     2401 cttgtgggat gaatatatgt ccacactcaa atgcagatac ttcttctatt ttatgagata
     2461 cattagttga taaaacatct aacatatttg gtattaaagt agttctcatt acagaagttt
     2521 cttctcctaa tgggttaagt aatttaacaa aatttctctt tgcattacct tctggtactc
     2581 ttatcttatc tactccctta gggcttacaa atgaatatgt taatatttca tttaatccta
     2641 cagcagttga attatctttt aaagcatcca tgaatttttg ttttgaagtt ttaattcctg
     2701 ctgtcgtatt tccttctaat tgtacagaag gtatattctc aaatccataa attctagcta
     2761 tttcttccca tacatcagct tcttgttcca tatctgttct aaagcttggt acatctattt
     2821 ctaatttatc attagctacc aaattacatt taaactctaa tgattctaaa attcctacaa
     2881 actgctccat tggtacatct acacctaata ggtggttaat tctttgagga tttactacca
     2941 attttttagg ttctggttta tttggatata catctacaac accttttaaa acttttcctg
     3001 ctcctaacat ttcaacaagt tgtgcagctc tatttgctgc tatctctgca agattagggt
     3061 ctaagtcttt ttcatttctt gaagatgctt ctgacctaat accaactttt ttagctgtca
     3121 ttcttatgtt ttctggtttg aaattggcac tttcaaaaag tacagtcttc gtattagaag
     3181 ttatttctga gttagcacca cccattacac cagctaaacc aagtgatttt tctccatttg
     3241 ttataactag catatctttg tctaatgttc tctcaacatc atctaatgtt acaagtttct
     3301 ctccatcttt agcatttctt actactattc ttccagtctc tacttgattt atatcaaaag
     3361 catgaagtgg ttgaccaagc tctaacatta cgaagtttgt tatatcgact atgttactta
     3421 taggtcttac tcctgcttct gtaagtcttc tttgcatcca atatggagaa ggttctattt
     3481 ttacatctgt aaccattcta gcaacatatc ttctacataa atctggatta tctatctcaa
     3541 ctttgaaatc tatctcttcg tcactttctt ttacttcgat ttcaggatat tttacttttg
     3601 ttcctatagt tgcagctgct tctctagcta tacccatcat gcatttacaa tcaggtctgt
     3661 ttgaagttaa ttcaaaatct attaaagcat cttttaatcc taaaacatct tttatatctt
     3721 ttcctaattc ataagaatct tcgtggtcta aaatatatat accacctctt ttatactcct
     3781 caatgtaatg ttcatctata cctagttcag ctgctgaaca catcatacca tctgataatt
     3841 cacctctgaa atcagtctgt ttgatttcaa ctcctccagg taacttagaa ccatttacag
     3901 ctactggaat ataatctcct tctgatatat ttgtagctcc tgtaactatt tgaacaactt
     3961 tatctccaat atctacttta gttacaacca acttatcagc atttggatgt tgttttattt
     4021 ctaaaatctt tccaaccaat atattttcta tttcttcacc ataataatct attgtttcaa
     4081 ctttagttcc tgtcattgtc attttatcag cgaactcttt tacatccatg tctatatcaa
     4141 catagtctct aagccatttt aaagatacta acatctacat accctcctaa ttaaaattga
     4201 tttaagaatc tcatatcatt ttcgaataat aatctaatat catctatttc gtatttaagc
     4261 attgcaagtc tttcaacccc aactccaaat gcaaatccac tgtaaacttc tgggtctatt
     4321 ccacaatttc taagcacatt tggatgaacc atacctgaac ctaatatttc tatccaacct
     4381 tcatatttac acattgggca acctttacca ccacatttga aacaagtaac atcaacctct
     4441 gcacttggtt ctgtaaatgg gaagttgtga ggtctaaact tagttttgat atcagaacca
     4501 aacaattttt caacgaagat atccatagtt cctttaaatt ctgccatagt aacatctttt
     4561 ccaacaacaa gcccttctat ctgatggaac attggtgagt gtgtagcatc tggcgagtct
     4621 gacctaaaac atctacctgg tgcaattact tttattggta actcttgact tctcatagtt
     4681 cttacttgaa ctggagatgt ttgagttcta agtaataccc catcattgat atagaatgta
     4741 tcgctcatat ctcttgatgg atggtcttta ggagcgttta atgcgtcaaa gttgttttca
     4801 acagtctcaa cttctggccc ttctgctata gagaatccca ttccgataaa tatatctgtt
     4861 acttcatcta taatttgagt tattggatgc ttcttcccaa cctttaaaac ttttcctggt
     4921 tgagtaacat ctatcacttc ctcttttaat tttctttctt tttcaatagc atttatttct
     4981 tcttttttag aatttatgct aagttcaatg ttttctctta cctcattggc aaccttacca
     5041 actactggtc tttcttcagc agataattta cccatttctt taagtatggc agttatctca
     5101 cctttttttc ctaagtactt aactcttaaa ctttctacac tttctatgct ttgtgcttct
     5161 tttatttcag ccaaagctgc ttcacgtaaa gcaagtaatt tttcttgcac acatatcacc
     5221 ctttcgtttt tttgtataaa aaaagcccct caatccctgt ctagggacga aaggctatag
     5281 tttcgcggta ccaccctagt agtttaacaa tttaaaaatc atcaaaccac ctctaacaaa
     5341 cttaacgcgt ttttgacgtc aaagcctact gtaaaatttc agctctgtta ctccagaggg
     5401 aacttctcat gacctcttag aaaacactcc cagcctaggt gtttatctct ttactaagtt
     5461 taatcaaaat actttcctct ttcattgcat ttacaatatt atattgttta ttttagtatt
     5521 atatcatttc cattcaaatt atgtgactat ttttaatata tatccaatac ata
//
LOCUS       DAMJYX010000168         5533 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJYX010000168 DAMJYX010000000
VERSION     DAMJYX010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.173"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC479_003370"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC479_003370"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="PC479_003371"
     CDS             complement(385..1569)
                     /locus_tag="PC479_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2805885.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="PC479_003372"
     CDS             1942..4293
                     /locus_tag="PC479_003372"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2805886.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="PC479_003373"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="PC479_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2805887.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          628 c          891 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAMJYX010000169         5494 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJYX010000169 DAMJYX010000000
VERSION     DAMJYX010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.174"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            94..1275
                     /locus_tag="PC479_003374"
     CDS             94..1275
                     /locus_tag="PC479_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2805888.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1417..2334)
                     /locus_tag="PC479_003375"
     CDS             complement(1417..2334)
                     /locus_tag="PC479_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805889.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2587..3381
                     /locus_tag="PC479_003376"
     CDS             2587..3381
                     /locus_tag="PC479_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805890.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3382..5442
                     /locus_tag="PC479_003377"
     CDS             3382..5442
                     /locus_tag="PC479_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2805891.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2103 a          625 c          911 g         1855 t
ORIGIN      
        1 aaatagaatg cttatagtgg taaaattaat tattagaata ttagaaataa taaataaata
       61 atacattgta cttaaaaggg ggagaaattt aagatgaaca aaaaatatat taaagcaggg
      121 aatttaattg atggaaccgg aaatagtatt atgcaaaata agggtataat tattgatgga
      181 gatactatag ttgaaattga agatataaga gatgatttaa atggatatga tgtgtacgat
      241 tacttagata aaactgttat gccaggcatt atgaattgtc atgttcactt gacaatggag
      301 ccagttggaa atccaaagat atattatgat aatgtatcag atgtagagct tgtagtaaat
      361 acgataaaac aacttgatgc ttatttggat tcaggagtaa catatattag aagcttaggt
      421 tgtcccaaat atgtggatgt acaacttaaa aatcttatag acaaagggtc tatagatggt
      481 cctggtatag ttacttcagg tccagttata tgtatgacag gagggcatgg atattatttt
      541 ggtattgaaa gtgatggagt agatgaatgt agaaaatcag ccagaacagt tttaaaaaat
      601 ggagtagatt gtgtaaagat aatggcaact ggaggtgtaa caactgatgg agttgagcca
      661 gggtcaccac aattaacttt ggaggaaatg aaggctgctg tagatgaagc tataaaagca
      721 ggtaaaataa ctgccacaca tgcacaagga agaactggta taaaaaatgc agtgcttgca
      781 ggaattactt ctatagaaca tggtgtttat ttagatgatg aaataattga tttgatgctt
      841 gaaagaggaa cttacttagt tccaactgtg gctgcaccat attttattct aaatgcagga
      901 aaagaaagtg gaataactga agctactctt agaaagtgtg agattgttgc cattccacac
      961 aaagagtcat tcctaaaagc atataaaaaa ggaattaaaa ttgcagttgg ttctgatgca
     1021 ggaacttctt ataatgaaca tggaaaaaca tattatgaaa tgaaactaat gtcagattat
     1081 ggtatggata atatggatat aatagttgct gctacaaaaa cagcatctga acttttaaga
     1141 attgataaaa attatggaac tctagaaaaa ggaaagaaag cagatgttat agttgtaaat
     1201 ggaaatccag tagagaatat agacgtactt gcagatgtgt tagcagtatt taaacttggt
     1261 aaaaaagtta gataaatagt ataaaaataa aaaagagtgt ctaaagaaat ttttaaataa
     1321 ttatagacac tcttttttat tagtaataat taaatctttt atactttaga aaatagattc
     1381 tattttatat ttagatataa ctctttaaaa tcattcttat gcaatataat ctatagaatc
     1441 tacacataca aagtatattc ttgaatcatt agaatttcca agtactataa catcattttt
     1501 cttacctaga acttcaacac cagttagaac caaactacca gctactacag taacttgatt
     1561 atttatattt gacatataaa gattatctag gataccttta ttacaacagc aatcatcaca
     1621 atcgcagttg caattacatt ctttgtaatg ttttttatca agatagtaac ctaaagatgc
     1681 tatgaaatct tcataatctg caggtgtaat atcaaatgca acagctttaa gattacacaa
     1741 tgatgctaaa tctacgtttg ctaatggagt agatgcagca agtataaaat caattatagc
     1801 agcaagtatt gcatctaata tagccccaag gtctatcgta ggaggaataa ctgcatcaat
     1861 aagtgcaatt aattcagcta ctcctggtat agttcctata gtattaatta atccctcaag
     1921 agttaaaggg actggaatag gataagatac tctaccagct atatctatta aatcacagtt
     1981 acaagcagaa aatctttcaa aagtaccatc aagtccagat aaattatctt ttggaggagc
     2041 tgaaagatct atacctacca aattagcacc tactataaag aaatctgaga taaaagcaaa
     2101 ttgattaaag tttacaaaag gtcttagagc atcatatctt agaagttcta atgcttctct
     2161 catacttttt ttacaacaac attgttttat tttaggttct ttatatttat ttggataatt
     2221 atagtatcta tcagctactt gataattttc atagtatctt tcatttgtat ttggataatc
     2281 ttcctcatat tcttgtgtaa atctaaagtc ctctctacat ttattatttt ccactaaata
     2341 cactcctttt atattttaat ttaaatgtat agataattaa gcttatctat atagtaatat
     2401 tatatttata atgtaaaaaa atgttacaaa agatattata aacgaataaa aaataataat
     2461 ttgtcagata aacttgtaaa tttttatgaa taatccattc taaaacaaaa aactgtgtat
     2521 aaaaaaggtt tatatgttat atttatataa atataacata tgaacgagaa aggagtggtt
     2581 tgataaatga ttgaggtaga taaattgtgt aagtctttta caagggttgt aaaagatgat
     2641 aagaataaaa gttcaataag aaagctaaag aaagtaaaaa ctaaaaaaga agaatttttt
     2701 gctgtaaata atgtttcatt taaagttatg gaaggtgaaa ttgttggaat attaggtcca
     2761 aatggtgcag gaaagactac attacttaga atgttaggtg gcatattaac accaacatca
     2821 ggaagtataa atatttctgg atatgattat tcaatagata gaaattcagc aaaaaaagaa
     2881 ataggttatc tctctggaaa tacaaaacta tatggaaggc tttctccgag agaacttcta
     2941 acaacttttg caagtctata tgagatgtca aaagaagata tagaagaatc aattgaaaat
     3001 attgtaaaaa taatggatat gagtgaattt atagataata gaattgaaaa tctatcaact
     3061 ggtcagactc aaagaacatc tatagctcgt tgtttaatac attctccaaa agtatatatt
     3121 tttgatgagc caacactagg tctggatgtt ttaagtagtg catctattat agattttatg
     3181 aagaatgaaa aactaagggg aaaaactgtt ctttactcaa ctcattatat ggaagaagca
     3241 gaaactttat gtgacaagat ttttatgatt cacaatggga aaataattgc atcaggaaca
     3301 ccacagagtt taaaagagga atctggagta aataatttga gagatgtatt cataaaactt
     3361 gccagaaagg aggacattta aatgagaagt aagattgtaa aatacatatt taaaaaagaa
     3421 atgttagaca tattgagaga taaaaagact ttatttatga tgattgttgt gccacttctt
     3481 ttatatcctt tacttatgtt attaatgatt caagtaatga atatgtctgc aagtagtatg
     3541 aatagtaagg acattaatat agcttttaat aataaaccta ataaggtgtt agtatccatg
     3601 attgaagatg ataatttaga aaaagatact aaaaatactt caaatgataa aggcaaaata
     3661 agagtgttag atgtagataa ctataaaaag tcattagaag aaaataaaat tgatgcttat
     3721 ataaagatgg aagaaaaaaa atcatctata aattatcaaa tatatataaa ttcttctcaa
     3781 gaaaattctt ctaatgcact aaaaagaatt gaaactgttt tggataaata taaaagattt
     3841 accatagaaa aaactttatc tgaaaaagga ttagatgtac aagctacttt agaaccaata
     3901 acatatagca ctgtagatgt tgcaaagacg gaagaagtgg caggatactt tttaggtcag
     3961 atacttccat ttatattaat aataggagtt ttactaggtt caatatatcc agcaatagat
     4021 gttatggctg gagaaaagga aagaggtaca ttagaaacat tatttacact gccaatatca
     4081 aacttagaac tggttatggg aaaatatatg gcagtttctc ttagtgcaat agtaacagca
     4141 attttaaaca ttttatctat gggactgact atggcattta ttttagttag tggaggtatt
     4201 agtagccaat ttggctttgg aaactttaat tatggagact tagtcttacc aattataact
     4261 actttaattt gcatatgtct tttttcaatg gtagtttcag caataagtat gtgtgtatgt
     4321 tcattagcaa aaagttttaa agatgctcaa aactacataa cacctcttat gttaattatt
     4381 atgataccat catatgtttc tatgatacca aatatagtat tagatagagt gacagctgta
     4441 atacctgtag taaatatatc tttacttata aaaagtgtat tatcttttaa aagcgattta
     4501 aatttaatag ccttagtttt agcatcaaat gtagcctttg ttatattggc aatcatacta
     4561 ctgtctaaga tgttcaattc tgaggaaata ctttttggta atagtaagag tttttctttc
     4621 ttagagaaaa gaagtaatat aaaaaaaggg tctataccaa ctgtaagtga tggtgttatt
     4681 ttatatgcag taggattagt tctattgatt tatgttggtt ctttagttca aattaaattt
     4741 ggtatggcag gtattataat gacacaattc atgataattt ctttaccatt attatttgca
     4801 tactatatta aagctgattt taaagaggta tttagcttga cagttcctaa attaaagcat
     4861 gtgtttggga gcattgttct ttggattggt ggatacatac ttattatgat tataacacag
     4921 ctaattttat tcttatttcc tcaaaatatg gaagttgctg aaagtttaaa taaggctctt
     4981 tttatcaaag atagtgtttt actgaattta ctagcaatag ctttattacc agctatatgt
     5041 gaagaaatgt tttttagggg atttatattt tcttcattta gtaaatcaaa agataaaaat
     5101 aagtctatca aactcgctat aatatgtagt ggggtactat ttggtattat gcatatggat
     5161 tttattagaa taattccaac atcaatatta ggtattatat ttgcatacag tgtgtataaa
     5221 tctggttcaa tatttgtatc aatgttattg cattttttaa ataatagtgt agcagttgtg
     5281 ttaaatcatt atacaactgg aaatataacc aacttatata aatttgttga agttgatttt
     5341 tcgaacttaa atgtgcttaa atttgcctta attcttctaa taagtcttat attaattatg
     5401 ttaggattaa gagttttaga tagaaagtct ttaagaaatt aagtataaag ttattatata
     5461 gattaaaata ttaataagca ctaaagtata tgga
//
LOCUS       DAMJYX010000170         5441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJYX010000170 DAMJYX010000000
VERSION     DAMJYX010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.175"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            99..2600
                     /locus_tag="PC479_003378"
     CDS             99..2600
                     /locus_tag="PC479_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2805892.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2760..3428
                     /locus_tag="PC479_003379"
     CDS             2760..3428
                     /locus_tag="PC479_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805893.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3412..4689
                     /locus_tag="PC479_003380"
     CDS             3412..4689
                     /locus_tag="PC479_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2805894.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5090..5311
                     /locus_tag="PC479_003381"
     CDS             5090..5311
                     /locus_tag="PC479_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805895.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         2291 a          552 c          966 g         1632 t
ORIGIN      
        1 aatatagatt tctaaataag taatttatca atattacata tcaaaataaa acgttacaaa
       61 ttagctgaaa atatattgaa agaaaggaag attttattat gagttcaaat aaagtggtag
      121 aaagttataa aattacaggt atgacatgtg cagcttgtgc aaaggcagta gagagagtaa
      181 ctaagaagat ggatggagta tatgaccaaa gtgtaaatat agctactgaa aaacttaaaa
      241 tagaatatga taattctaag gttaattttg atgatataaa gcaggttgta gaaaaagcag
      301 gatatggaat tataaaggaa gaaagtaata aaaaaattga tatgaaaatt gatggaatga
      361 catgtgcagc ttgtgcaaaa gctgtagaga gagtggtaaa aaaattggat ggagtagaaa
      421 gtattagtgt gaatattgca acagataaag ctaatataga ctatgaccca tcaaaagtaa
      481 aattatctca aataaaagca gctattgaaa aagctggata taaaccaata gaagaggtaa
      541 aaaataaggt tgatgtggat gaagataaat tgagaaaaga aagagaaatg aagtctttat
      601 ttgttaaatt tatagttgca atagtatttg cagtacctct gttttatata gcaatgggac
      661 ctatgataat aaagcctata gggccatggc ctttgccaga aattataaat cctatgacaa
      721 atacttttaa ttatgcatta attcagttaa ttttagtaat accagtaatg atagctggtt
      781 ataaatttta tataaatggg tttaaatcac ttttttcttt aagccctaat atggattctt
      841 tagttgcaat aggaacatta gccgcatttt tatatagtct atatacgaca ctacaaatag
      901 ccaatggaca aattcaaggt atgcaccatc atcagttgta ttatgaaagt gcaggaataa
      961 taatagcact tatcttactt ggtaaatatt tagaatcaaa atcaaaagga aaaacatcag
     1021 aagcaattaa aaagcttatg gggctacaac ctaaaactgc aatagtgtta gttgatggta
     1081 aagaagttga aactcctata gaagaagtag agataggaga tatactatta gtaaaaccag
     1141 gaactaaaat acctgtggat ggagttgtaa tagaaggata tacatcagtt gatgaatcaa
     1201 tgcttacagg tgaaagtata ccagtagaaa agaatgttgg aagtaaggtg acaggagcta
     1261 gtattaataa aaatggtgtt attaagttta aggctgaaaa aattggtgga gatactgctc
     1321 ttgcccaaat aataaagtta gttgaagatg cacaaggtac aaaagcacct attgctaaac
     1381 ttgccgatac tgtatctgga tattttgtac caatagtaat tgcaatagct gtagttgcat
     1441 cattattgtg gtttttaata ggaggaaaag atatagtatt tgtacttact atatttatat
     1501 cagtacttgt aattgcttgt ccttgtgcat taggattagc aacaccaact gctataatgg
     1561 ttgggactgg aaagggcgca gaaaatggaa tcttgataaa aggtggagag gcattagaat
     1621 ctgcacataa agtaaataca gtgatatttg ataaaacagg tactataaca gaaggaaagc
     1681 caaaagtaac agatatagta ttaaataata atgtaaaaga agaatatctt atcaagattg
     1741 cttcaagtgc agaaaaaggt tcagaacatc ctcttggaga agcaatagtt aagtatggag
     1801 aagagaagaa tataaaattt gaaaaagttg ataattttaa agccatacca ggtgctggta
     1861 tacaagttac tattaatgat gaaagtatat tacttggaaa tagaaagctt atgaatgaca
     1921 ataatataaa gcttggggat ttagaagaaa aatcaaatat attggcaagc caaggtaaaa
     1981 cacctatgta tatagctgta gatggaaatt tatcaggtat aatagcagtt gcagatgtag
     2041 tcaaagaaag tagtaaaaaa gcaatagaaa tacttcatga tatgggaata aaagttgcaa
     2101 tggtaactgg agataatgcg aaaactgcaa atgctatagc aaatcaagtc ggtatagata
     2161 tggttttggc agaggtttta ccagaagata agtcaaaaga agttgaaaaa ctacaaaatc
     2221 aaggaaagtt tgttgcaatg gtaggggatg gaataaatga tgccccagct cttgcaaaag
     2281 cagatatagg gatagcaata ggaagtggaa cggatgtagc cattgaatct gcggatatag
     2341 tacttatgaa gagtgattta attgatgttc caacagcaat taaacttagt catgaaacta
     2401 taaaaaatat aaaacaaaac ctattttggg catttggata taatacaatt ggtatacctg
     2461 tagcagcagg aatattatat gtatttggag gacctttatt aaacccaatg attgcagcag
     2521 cagcaatgag cttaagttca gtttcagtcg tatcaaatgc acttagactt aaaaacttca
     2581 aggcatataa gagagattag attaataaat taaataaaat gtatccaaaa gagctatctt
     2641 taaattaaaa ggattaattt acaaattaag atagctcttt tttcatgtaa aattcacaac
     2701 aataagataa aatataagta atatacgaaa ttttaaataa aatagaaagg attgatagaa
     2761 tgaattcgtc aatacttgta attgaagatg attcaaatat acaagagtta atatcagaat
     2821 ttttaagtgc agaaggttat caagttgata cagcaaatga tgggttggaa ggtatacaaa
     2881 agtttaagca aggaagttat gatttagtaa tattagatat aatgatgcca aacttagatg
     2941 gttatggagt ttgtaaaatg ataagaaagt catcaagcgt tccaattata tttttaactg
     3001 ctttaaatga tgaaggagac caacttaaag ggtttgactt agaatgtgat gattatataa
     3061 caaaaccatt ttcatttaat cttcttataa aaagagtaga agcaatttta aggcgaagta
     3121 acaagactat aaatgacaaa tttatagttt ttgaaaaatt aaaattagat ttaaacacat
     3181 atattgcaga gatagatgga gaacctatag aacttacact aaaagagttt aatatattaa
     3241 aagctttgat agaaaagtat ccacaagtta taactaggga aggtcttttg gatagtatat
     3301 ggggttatga ctattatgga gatactagga ttgttgatgc acatataaaa aatataagaa
     3361 aaaaaatatc attaccatat attaaaactg taaaaggaat aggatataca cttgagaagg
     3421 atatttgata agtgggaaaa gttaagtata aagtataagt tattttcaat aacgacatca
     3481 ttattaatag cattagcttt aataatatat ctaattttat attttttatt gccatcatat
     3541 tatcatgagt ataaaattga gtctcttcaa gaaagtttga aatctttagt agatagttca
     3601 attcatttcg atacatatac tttagaagaa agactttatt atatggctaa agaccaaaat
     3661 ttggctattt tattaaaaga taaccaaggt aaaattgtat atggtaagaa tgaagtagtt
     3721 attttaagat atagtaagta catgattaat agtcttgagg atgagtatag aacttctata
     3781 cctatatata ctaaagatgc aaaagatggt ccatatactt tagaactcgt tatgccatta
     3841 caaccaattg acgaagcaaa cgaagttata agaaaactaa tgccatacat catatcaata
     3901 gcaatattga tagcgattat tggagcatat atatattcaa ttgtaataac aaagcctctt
     3961 ataaacataa tagagagtga gagagaacag gaatatagaa ggaaggattt tgttgctaca
     4021 atatcacatg aactaaaaac tccaataacc ataataagtg gtcaaattga aggtatgatt
     4081 tatagtgtag gtaagtataa ggatagggat acttatctga aaaaatcgta tgagtgtaca
     4141 caagaattaa aagatttagt aaatgaaatg atagaggttt caaaatcaga aatacttgaa
     4201 aaagatttga aattggtatc aataaatata agtgaattat taaatagact tgtaaagaga
     4261 caagtatttt taatagaaga aaaacatatg aaaactattt taaaaataga agaaaatttg
     4321 gaaattaaag cagaccaaga gagaataact aaggcaatca ataatataat aaataatgcc
     4381 ataaaatatt ctcctgaaga atcagagctt ataattagac tctatgataa aaataaacga
     4441 ataagtaaaa agacttctaa tcaaagagtg gttttagaaa ttgaaaatac tggtgtaact
     4501 atcgagaaac gttatttaga agaaatattt aatccatttt atagaataga aaaatctcgt
     4561 tcgagaaaaa ctggaggaag tggacttgga ctttatatag tcagccaaat ttttaaaagt
     4621 catgggtttg attatagcat aaaaaacaaa gaaaactctg tagtattcac agtagaattt
     4681 aaaaattaat tacttattat aaagaatata taactttcaa taatagttca ttttaaaatc
     4741 acaaaaaatt cataatgaaa ctgtattata taaatataaa taagcttaaa accataaaat
     4801 acaccaacta taatgatgag atttccttac atcctccata taagtcatca ttatagtaac
     4861 ccaacaatta agttactatg aatttaaaaa ataacaccga gcatataatc tatgcttagt
     4921 gttattttta ttttgaaata aaaaatattt aatttattga aatgtataga tttagatagt
     4981 ttaaactata tttaagactt aaaaattata attgatatta caaataaata tatgtacttt
     5041 gggaaaaata aatataggaa gtattatatt tacagaggag gtagttttga tggatagatt
     5101 aaaaagagat atgaaaaata tgaaagaaga tttaaaagaa ggtgttaaaa agacagctta
     5161 tgttatgaaa aatacagctg aagatttgaa agatgatgca caaggtgcta tggtaaactt
     5221 agaaatgaaa aaagatgaga tgatggaaag atatgaacaa aagaaatttt ctaaagaatt
     5281 agagaagcaa atgaagaatg aaatgaaata agcttttatt tatgaataac aaattattgt
     5341 attcatataa aaagtagcca gtaatattaa atagtttatt taaggaaata gactttctaa
     5401 aaataactgt attagattta gaaagtctat ttcaaattag t
//
LOCUS       DAMJYX010000171         5354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJYX010000171 DAMJYX010000000
VERSION     DAMJYX010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.176"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            165..662
                     /locus_tag="PC479_003382"
     CDS             165..662
                     /locus_tag="PC479_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2805896.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(838..1086)
                     /locus_tag="PC479_003383"
     CDS             complement(838..1086)
                     /locus_tag="PC479_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805897.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(1152..2168)
                     /locus_tag="PC479_003384"
     CDS             complement(1152..2168)
                     /locus_tag="PC479_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2805898.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(2226..2993)
                     /locus_tag="PC479_003385"
     CDS             complement(2226..2993)
                     /locus_tag="PC479_003385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2805899.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(3061..5250)
                     /locus_tag="PC479_003386"
     CDS             complement(3061..5250)
                     /locus_tag="PC479_003386"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2805900.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         1648 a         1023 c          529 g         2154 t
ORIGIN      
        1 atttttctct ttagtttcac acatcaccaa gtctaaactc tatattatat attaatattt
       61 ttgacttgtc aagttattta tgttataata ttaatcatta gataattaaa gcattgtacc
      121 taagttgcaa tatctaaaat tcaatttgga ggtataacta gcatatgtat aaaattggtg
      181 aaagtgtaat gtatccaaaa gagggagcct gctctgtaaa tgatattgta acaaagaaaa
      241 taaatcatga gatgcaaaaa tactatgaat taagtgtcat ttttaatagt aaccttaaaa
      301 tttcgattcc agttctaaat gcagatagaa ttggcatacg tccagttatg gatggaaatg
      361 atgttgataa ttttatccaa tctattaata aaacagatgg tgtatggatt tttgatagaa
      421 aagaaagact taaactgtac caagataaat ttcattcagg agatgttttt gaaattgtaa
      481 aattaatcaa aatgcttatg atacaagaca gttcaaaaca attgtgtagc acagataagg
      541 agtttttaaa taaagctcaa aaatttgctt tatcagaatt agctgctgct caatgtaaat
      601 cctatacaat ggtattagag gaaatgaaaa aacatatatt aaattcaaag aatacaaatt
      661 aaaccagttt tttaaactgg ttttattttt tatactaatc atccatgcta ctttagctta
      721 ttttaaactg cctaatctaa tattaataaa gttatctcaa ttagatagat gtattttatt
      781 caaataaaaa aaactaagag taaatcatat tactgattta ctcttagcta ataaaattta
      841 atcttcttca agttcttttt tctcttgtaa taatacactt aattcatttg cttctaaaac
      901 ttgtgcctct gcaatatcaa gtaactcttg ttgccattcc acacatttat ctttgtaatc
      961 aaaaggtagc gttctgtcaa ctatgtactt atcaatacag tcctccgcac ttttaattat
     1021 ttcttttctt tcatcaataa atttttctaa ttctactaag gcttcttcat aagcttttct
     1081 actcatataa attccccctt tgtacaatta ttctacatta aaatgatttt tcctcttttt
     1141 tgtataaaat tttatttgta tataactttt ccatcaatat cttttatagt ttgacatcca
     1201 gttaatatca tagaactact tagctctgat tgaagtttat ttacataagt ttctacacca
     1261 tcagttgcac caccaaaaga agcagtcaca aaaggtcttc ctattaatac tgcatctgca
     1321 cctaaaccta tcatttttag tacgtctaaa ccagttctca ctccaccatc tgcaagtatt
     1381 gtaactttac ctttaacagc atcagcaatt tttggtaaaa cttcacatgc tcctggtgta
     1441 cagtcaagca ctcttcctcc atgatttgat acgactattg catccacacc tgcttctact
     1501 gccattaaag catcttcaac agtcattata cctttcaata taaatggtag tttagtagac
     1561 tttactaact cctttatttg tttaacattt ttaggaagta ctggttttcc atgtaatgat
     1621 aaagttacta gaccacaggc atctatatca actccaactg caaatgcacc agcttcttca
     1681 gctaatctta tcttttttat aatattttca ttatcccaag gtttaataaa tacaattcca
     1741 gctccattat actttttgac tacatctaaa ttttccatta aaaaagcatc tactgccgta
     1801 tcaccaacca ttgcataaat accactatta acacatcctg caactactgg ttctatgtaa
     1861 tctctttcat taatttttcc acccatattt aaagtagttc ctgttactgg agctgcaaat
     1921 ataggtgcac tcattttctt tccaaacatc tctatagatg tatcaggatt tgatacatca
     1981 tgtattactc tcatatttac ttttactttt tctaaactct tgctattttc tataaaagaa
     2041 gaaccacttc ctttacctcc catgccaggc acttcacctg cacaagcaac cccattacaa
     2101 accttacaaa ctttacaact tccatttaaa ttttctctag cactttttaa taattcatta
     2161 tattccatct ttaatccccc attaactata gtaatttata ttatatacat tatatcatat
     2221 tatatttaca gtatattttt caaaaaactt tttctgtgga taggagttat gccatatttt
     2281 tcaatagctt catagtgttc tttagttcca tatcctttgt gagatttaaa cccatattct
     2341 gggtatactc tatcatattg gtacattata ctatccctag taactttagc aagtatactt
     2401 gctgctgcta ttgatataga ttttgaatct cctttcacaa ttggattttg agatatgtca
     2461 atacctggta ttgttgctgc atcaactaat aaataatccg gtgctttttt taaacaattt
     2521 atagcctttt tcattgccat atatgtagca tttaatatat taaattcatc tatttcttca
     2581 ttgtttacta tacctattcc ataatctagt gcttcttctt ttattacttc aaaaagttca
     2641 tctctttttg cctcactcag tttcttagag tcatttacac cttctatttt ggtatctttt
     2701 ttaaatacta caacagaagc tacaacaggt ccagcaagtg gtcctcttcc cgcctcatca
     2761 attcctccaa tatataaata acctttatca tagccttcgt tctcaaatat atttatagtt
     2821 tccaatcttt cttcttcttt tctaatatta tccagctttt ttgccagttt tatagctaga
     2881 ccttgtacag attttctttc atcaactctt aacagctcta tatattccat atatttttct
     2941 acttctaaag tctctattat ttctttaatc tccctcacac ttttatcctg cataccattc
     3001 cctcatcttt cgtttttaaa aatttaattt ttaaatatat taaaattttt taattagagt
     3061 ttaattgcat ccactatagc cacattttaa acatatacta catccacctg tatgtgcaat
     3121 tccttcactt ccacactcag ggcacacaaa tttttcatct ccttcaaaat ttttatttat
     3181 tatttctttg gctttttcat ctttattatc tttttctttt ttattatttt tataatattc
     3241 ttttttacta ttttcttttt tatcttcttt attattttct tccttttttt catttattaa
     3301 aattccactc cttttacatc catctctaaa tattgttact cctttaagac cagcctccca
     3361 tgccttaata tatatattga aaacatcttc aactgtagtt tcaaaaggta tatttattgt
     3421 agatgatata gaagcatcaa tatacttttg ccatactttt tgcatgttta ttctttcttc
     3481 atatcctata ttcatagcag taacaaaaaa atctggtaaa cattcttcat ctgtaatacc
     3541 tttcttgtcc atatactctt taacaattgg agtatatact ttataataca catcctcatc
     3601 atgcaagctt tcagtttttc ttatatatga aagattaaat ataggttcta ttccaccact
     3661 gatacctatc attgtagata tggaacctgt aggaggtatg gttaaaagtt gtgaatttct
     3721 aagcccatac tttttaacca tttccttagt gtcttcattt agattttcta taaaaaactt
     3781 agatttagaa atattttcat atttatactc actaaatgtt ccatattctt tagcaattaa
     3841 tgctgattgc ttcacagctt catttaacat agtcttacca attaaatcac atagttctaa
     3901 agattcatca gaaccatatc ttattcccat ttttataagc atatcagcta tacccattac
     3961 acctaaacct atctgtctat acttatgtac actatctctt tgttcttgta atggatgcaa
     4021 gttcaatccc tcatctaaga catcatttaa agctactaca gcttctctaa cgcaagattt
     4081 taatttatta atatcaaaag tagcatgttc accaaatggt tgtattataa actctgacaa
     4141 gtttattgag ccaagtagac aacttcctcc acttggaagt ggttcttctg cacaaggatt
     4201 tactcctgaa aagcaaaagt tcttatcttc actcataaaa ttatatgttt ttattttatc
     4261 ccaaaacaat atacctggtt ctgcataatc ccagttattt ttcacaaatt tattaaataa
     4321 atcataagca tttactatct taactatttt ttctccagta gtttcaactt caaaagtaca
     4381 attatataac tttttttctc ttacagcttg cataaattca tctgttactc ttacagaaat
     4441 atttgcttta gtaaccttct ccaaatctgt ttttatatct ataaagtctt ctatctctgg
     4501 atgatttaca tccatagaaa tcataagagc tcctcttctc cctctttgac caattaagcc
     4561 agttgtcata ctgtaaagtt ccataaaaga gactgctcca gtcgtatgtt ttgcagcatt
     4621 gttaactttc gctccttttg gtcttagctt tgaaatatca attccaactc ctccaccata
     4681 actaaaagtt ctagcaagtt tttttgcagt atcaaatata tcttctatgc tatcttttgg
     4741 tggttctatt acataacaat tagaatatgt tacttttttt ccctctttct gtaatcctct
     4801 atttgctaaa attcttccag caaatagaaa ttttttttgt cttattagtt ttgatattct
     4861 tgaactatta tttgaaattc tctttatcca ttcatcaaaa ctctcaccat tatgtctata
     4921 cttgttcttc catattgatt tttgtaattc atcaaactcc caagctttag tccttatttc
     4981 agagcgtctt tctctatata atatgtagtg ttttgctata tcttttctat ttgattccat
     5041 aagtagtact tcaactatat cttgtatttc ttctacggat aagcattttt ccatagaatt
     5101 atagtcatta gctatttttc ttgatatacc atatgccaac tctctatcta ctccaccttc
     5161 actatttata ctagctttat agacagcatc ttcaatctta cttttactaa aaagttcttt
     5221 tgtaccatct ctttttatta tgtatttcat ttttaaacct ccaataaatt agtattaaat
     5281 taatacacac attaactata actctaaaca aactttttat atatttgtat attttcatat
     5341 taattatcta acat
//
LOCUS       DAMJYX010000172         5205 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJYX010000172 DAMJYX010000000
VERSION     DAMJYX010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.177"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            71..1534
                     /locus_tag="PC479_003387"
     CDS             71..1534
                     /locus_tag="PC479_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2805901.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1590..2726
                     /locus_tag="PC479_003388"
     CDS             1590..2726
                     /locus_tag="PC479_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2805902.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2962..4077
                     /locus_tag="PC479_003389"
     CDS             2962..4077
                     /locus_tag="PC479_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2805903.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4229..5098
                     /locus_tag="PC479_003390"
     CDS             4229..5098
                     /locus_tag="PC479_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2805904.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2001 a          539 c          983 g         1682 t
ORIGIN      
        1 tatataaaat attgaaatat agctgtttaa attattgtct ataaatataa aatgaatggt
       61 ggtgagttaa gtgaccgatt tagttaacac cattaatgga attgtttggt cacccgtatt
      121 agttgcaatg tgtttgtgta taggattata tttttcattg aaattaaaat tttttcaaat
      181 aagagatgta aaagaaatgt ttactctctt gcttgaggga gatagttctc atgttggtat
      241 atcatcattt caaggttttg ctacatctat ggctggaaga atagggactg gaaatatagc
      301 tggtgtagct gtagcaatag caatgggagg tccaggagca ttagtatgga tgtgtataat
      361 ggctacatta ggttcagcaa ctgcatttgt agaatctact ttagctcaaa tatataaaga
      421 tgagcatgat gggcagtata gaggaggacc tccatattat tttgaaaaag gtcttgggtg
      481 gaaaaagtgt tcagttttat tttcaatagt tgcaataata agttatacat tttttatgcc
      541 tggtacacaa tcaaacactt tatatctagc aatgaatcaa gcatttggat tttcaaaaat
      601 gacaatagca gtagcatctt ctattgcatt agcgataata atttttggag gaattaaaag
      661 gattggttta tttgctgaaa aagtagtacc tataatgggt ggagcatacc ttattatgac
      721 attagtaata attataacaa atattagttt agttccagag gctattgtaa caatgtttaa
      781 atctgcattt ggatttggtt cagtttttgg aggaatgcta ggttcagcaa tatcttgggg
      841 agtaaaaaga ggtatatact caaatgaagc aggagaaggt tctgggactt atggtgcggc
      901 agcagcagaa gtaagtcatc cagccaaaca aggattagtt caagcatttt ctgtttatgt
      961 agacacatta ttaatatgta tggctacagg tattatgatt gtaataacag gaatgtataa
     1021 tgttacaggt gtttgcacag cactaccagg tgtagaaccg ggaccacaat atgtacagag
     1081 tgcaataaat acattacatc cacaattagg tgatattttt atagcaatag ttttattatt
     1141 atttacattt acaactttat tagccttctc atatatgatg gagactaatg tatcttactt
     1201 agtaactaga attaaagcag aaaaacatga aaagtacata attaatgtct gtagatttat
     1261 gcttatagca tgtgcagcaa tgtgttgttt tatagaacca cttacaagct gggcattagg
     1321 tgatatatgt ataggtgtat taacatatat aaatttaatt gccatcttta cactaaaaaa
     1381 accagctata aaggcatatt atgattatat aagacagaag aagatgggaa taaaaaggag
     1441 tgatagaact tttaacccta tagagttaga tataagaaat gctgagtttt gggaaaatag
     1501 atacttaaaa gaggataagc atgagttaaa ttaaagttat aataaaatat aaaaattact
     1561 aaataaattt agtgggaggt aagattggta tgaaagaaag aataatacaa gtggctgatt
     1621 caaagaaaga aaaaattcta gggctatgtc aatctttata tgatgaacca gaaattgctt
     1681 tgcaagaata taaatctgct aaaaaaatat cagaatttct aagagaagag ggttttgatg
     1741 ttgaggaaaa cttggcaggt atggctacag cttttaaagc aaccaagaaa aatggagatg
     1801 gtcctaaaat agcatttata gcagaatatg atgctcttcc aggcaatgga catgcatgtg
     1861 gacatcattt aattgcatct atgggagttg gagctggaat agctctttca agtatactgg
     1921 atacttacaa gggagaagta agtataattg gaacacctgc tgaagaaact ggagatggaa
     1981 agccttactt gatagaacat ggtgtatttg atggctatga tgcagcaatg atgatacatc
     2041 caaattcaaa aacttgtgta acacctgaaa ttatagcaat aggaggtcta gatttcatat
     2101 ttactggaaa agcatctcat gcaggagcta agccatataa cggaataaat gctttagatg
     2161 cagtagtact actatataat aatataaatg cactaagaca acaattagta gatggaacga
     2221 gaatacatgg tattatatta gaagcaggaa ctgctgcaaa tgtaatacct gatatgggaa
     2281 aagtaagatt agagataagg gcaaaagaac aaaattactt tgatgaagta gtagaaaaag
     2341 tgaaaaattg tgctagaggt gctgcaatag ccactggatg tgaacttgaa ttttatcact
     2401 ttgaaccaac ttgccaagga ttaaatgaaa ataaagtttt ggtagacata tttacaaaga
     2461 ttatggagga atttggaata tatgaagatg aacaagtaat gttaggttca acagatatgg
     2521 gaaatttaag tcaaataatg ccatgtatac atcctttgat gaagttttct gaaaatggag
     2581 aagaactaca tacaaaagaa tttttggagg cttctataaa ttcatatgca aaagatagag
     2641 taattgatgg tataaaaata ttagctttaa caggatttaa tttatttgaa aatccagaat
     2701 tattgaaaaa aatgaaggaa gagtagaact tagactattt ttaattatac tctttaaaat
     2761 aagaagagtg tctcaaaatg aactttttag tttatgagac actctttttt gtatgaaatt
     2821 aaatatattt ttattatttt aaacatgaaa aagaggctta tctgttttta tatgaccttt
     2881 tttaacttgt agcacaaaat atcatgaatg attacaattc gaatgataaa atcgattata
     2941 agatatatag gaggggtaca tatggaaact ttagaagtaa gaaatattga attgagtggt
     3001 acaaattatg agataggata cagactagga gaattggttg ctaatatgcc agagataata
     3061 gaaggtcaga ttaataaatc taatctggta agcaaaaaag aagaaaaaga gatgatagag
     3121 ttgtttgata aatattgtcc aggtcttaat gaagaattgc aaggttttgc tgatgcaatt
     3181 caagtgaatt gtaatcaaat tttatattat actatgactt atcttaaacc aggttgtagt
     3241 caagttgcat tagcaccaga attaactgaa aatgggcatg tactatttgc acgtaatttc
     3301 gatttctcac acaatatgga ggattttgtt ttatgtaaaa ctaaggtaaa tggaaaatat
     3361 gcacatatag gtactaccat tatgcaattt ggaagaggtg aaggtatgaa tgaatgtggc
     3421 ttaggtgtaa gtcagtcatc atgtggtatt cctgtaggca attcagatgg tttaagaaaa
     3481 ccagcaattg taggtttaca gttttgggct gttattagat atttgttaga aaattgcaaa
     3541 gatgtagatg aagcattaga atatttaaaa gatatgccaa ttgcatacaa tattaattta
     3601 ttgcttgcag ataagtctgg aaacattgca cttgtggaga ctttagatgg gaaaaaagaa
     3661 gtaaatataa tcaacagttt agaaagtaaa agagaatatt ttttacactc tactaatcat
     3721 acacatattg ataaacttca taaattagat ccacaatcaa tgaaaaattc tattcataga
     3781 tataaattaa ttaaagaata tataaataag tctaaaaaaa ttggagagaa agagttaatg
     3841 aatttattgt cttcaaaata tccaaatggc ttatcttgta attactataa tgattttttt
     3901 ggaactttaa aaagtatagt tatggattta aatataggaa agtttaatat tttatggggt
     3961 ggactagaga ataaatggga gagctactat ttaaaaaatg acattaaatc tataacacag
     4021 aggataaaca taaatattga aaaagcacca tctgactttt ttgatttcat tgaataaaaa
     4081 atgtatttaa ttattctaaa taattaggac atataaatca actaaaagtt tttatttatc
     4141 gtattgaatt agttataata ttttagtata gtaaaaaata gtgctatttt acatatttaa
     4201 agagtttaag tagaagagag gtgccatatt ggattactta aagtattttg aaaaagctgt
     4261 aatgtatatt gagaataatt tacatgaaaa tataactgta aatgatgtgg caaaagaaac
     4321 tggatattct tattatcatt taacacgctt atttaagtct atgtttggtg aaagtgtagg
     4381 aagttatata aaaaaaagac gattagtaag tagtacaaaa gaacttttat atagtgataa
     4441 aaaagtgata gatatagcta ttggtagtgg atttgaatct tcagaagcat ttagcagagc
     4501 atttaaatct atatacaagg ttagccctat agaatataga aaaaatagaa ttgatgtatt
     4561 tgttggtaaa aagaaaaaat tagagttaga ttttatgaga catcttgttg gaaatataac
     4621 gattaaacca gttataaaag aaatagaaaa aataaaggta ataggtataa aggataaagt
     4681 aattttagaa aataattcat tacctgactt gtgggagaaa ttcagaaaag tacatcatat
     4741 tgtcccaaat actttgccat caaggagagt ttttggaatc tgtgaagcaa tctctgaaat
     4801 acacctagtt agtgaaagta tggagtttaa tgagataata ggacttgaag ttaatagtta
     4861 tgatgtgatt ccaaattcat ttgtttcaaa aactattaag gaaggaaagt atgctatatt
     4921 tactcatact ggtagcttag ataatttaga caaaacttat gaatatatat ggggtacttg
     4981 gtttttaagt tcaaaagaag aacttgatat aagagatgat tttgaagtat atgatgaacg
     5041 atttttaggt cctagtaatg tgaactcaca aattgatatt tatataccta taaaataatc
     5101 tataagaaaa ctaaaagata atttgattaa gaaatagatg ttcgtaaaag agcatctatt
     5161 ttttattttt ataagattta gataatttta ataataaaag aaata
//
LOCUS       DAMJYX010000173         5154 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMJYX010000173 DAMJYX010000000
VERSION     DAMJYX010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.178"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(4..540)
                     /locus_tag="PC479_003391"
     CDS             complement(4..540)
                     /locus_tag="PC479_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2805905.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(653..1327)
                     /locus_tag="PC479_003392"
     CDS             complement(653..1327)
                     /locus_tag="PC479_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2805906.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            1530..3404
                     /gene="asnB"
                     /locus_tag="PC479_003393"
     CDS             1530..3404
                     /gene="asnB"
                     /locus_tag="PC479_003393"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2805907.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3496..4932)
                     /locus_tag="PC479_003394"
     CDS             complement(3496..4932)
                     /locus_tag="PC479_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2805908.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2000 a          614 c          741 g         1799 t
ORIGIN      
        1 caactataat aaaatataaa attgatatcc aatcaaaaaa ataactatga aatttaatag
       61 cttagatatg aatttttctg tttttgagtt agtttttata ggtagtaaag aaatgtttat
      121 ggtaatggga taaaacaatt caataccttc tttagtaata aggtcaagac acaagtgaga
      181 tagatatcct attataaacc cactggacaa acatgagaaa actatattat tgttagtgaa
      241 gattgtcaga gatttaaaaa aactataaat aagtaataag gcaattagac tgtgagtaaa
      301 ccctctatgt ctaaacctac ttccaaataa tttgtatatt aacataaatt ttttacttat
      361 gtaagaacct ctcatatcta tatctggaaa taaagaacct aaatatgcaa aataaatata
      421 aattataatt aatactattt tatatgcaat atcgtattta actaggtata gattgtttat
      481 aaaggggagt atcaaaagtg cgaatatata acctcccctt tcatgtgttt cttttatcat
      541 ataaaaatcc tcctgaatcg tcactataaa cattatattg gtaaatttag actatctttt
      601 attatattat atagcaaaca taagtttttt tcaaaaggat tttaatattt aattaatctt
      661 gagattttaa atttttgtaa ttttctaaag caccattcca atttactaca ttaaaccagt
      721 tgtcaatgta ttcatttctt ctattttgat attttaaata gtaagcatgc tcccaaacat
      781 caagtcctat tataggagtt aggtttttac ttacagggct atcctgattt ggagtagtca
      841 taatagataa tttgccatct ttagtagcta caagccaagc ccaaccagaa ccaaagacat
      901 ctaaagcaga tttttggaac tcttgcttaa atttttcaaa agaaccaaag tctctatcaa
      961 tagcttcttt taaagattca gaaggtatgg ttttttctgg cgtcattata tcaaaaaaga
     1021 atttatgatt ataagctcca cctgcattat ttcttacagt tgtagcaata tctttaggta
     1081 aagaatctaa attttgcaat aattcacata aagaataatt ataaagctca ggatattttt
     1141 caagagcagc atttaattta tcaacataag cttgataatg cttatcatga tgcagtttca
     1201 ttgtttcttt atctatataa ggttcaagtg catcatatgc ataaggtaat ggtttaacct
     1261 taaatttgtt attttcaggt gtaaaagcaa atgaagttat gcactgtgag attataaata
     1321 aagacataat aactggtatt aatatttttt tcttcataaa aatcacctcc taatatagta
     1381 tttctagaaa taaaaaaaat atgaaatatt aacagataat ttataagaat ttaatagcaa
     1441 tttcatttta aattgaataa gttttaaagc caaagacaaa actatgaaat atagaataag
     1501 actaaaggac agaaaggaat tttgattata tggacggcaa tatttcaatt tattataaag
     1561 gtgatatatc taaaaaagaa gatattaaat ttaaaataaa agaatctgta aataatacaa
     1621 gactttctag tagaatagag tataaggatg aggaaaaaac ttttgatttt gaaagattta
     1681 atgtaattga tgctatttat ggaaataaac catttgaaaa agatggaaaa gttattgttt
     1741 ttaatggaaa tatatataac tatcatgaaa taaaagaaga attaattaaa aaaggacaca
     1801 attttactac aaatgatgat attgaagtct tattagcatc ttatatagaa tttggtaaga
     1861 attgtgtaca taagattaaa ggaatgttca actttataat ctatgataga gaaaatgaaa
     1921 gtatatttgg agcaagagat ttatttggga taaaaccact ttattatata aataaggaaa
     1981 atgcaataat tttttcctca gagtataagt ttttacttga atatatgaaa aatttaaata
     2041 taaatgaaag aagcttacaa agttattttt catttcaata tgtacttcct gaagatacta
     2101 tgatacaagg aataaggctg ataccagcag gtcactattt tagggtagaa aatggtatac
     2161 tatctttaaa gagatataat aaacttgagt ttagaagtag tacaaaattt ttttatacta
     2221 aaaatcactt aggcaataga gatgtgaatg aggatgatat aagaaatata gtagtagatt
     2281 caatagtaac tcatatggaa gaggagaaag aaattggaac atttttgtct ggtggaatag
     2341 attcatctat tataactact gtggcttcac aaattaatcc aaatataaaa tctttttctg
     2401 caggctttag tgtcaaagga tatagtgaat tagaagtagc aaagaaaaca gctgataagt
     2461 taggtataga aaatatccag attaatataa cacaagatga atatataaaa tcattaccga
     2521 atgtaattta ttctttggat gaccctatag cagacccttc agaagtaggt ctttatttct
     2581 taattaaaga ggcaggtaaa catgttaaag tagcattgtc tggagaagga gcagatgaat
     2641 tatttggagg atataatata tataaagagt atagtactat gaaatcagtt gtcaattcac
     2701 ctacttatat taaaagtata ttaggaagag tttcagaact tatgccaaac ataaaaggaa
     2761 gaaattatct ttatagagct acaacaccat tagaaaaaag atatatagga aatgcaaaag
     2821 tttttgaaaa cagcgaagta aaaagattct tttttaagta taaggaaaag aatatatatg
     2881 aatacctgtt gtctaatttg tatagagatg cacaaaaaaa taattacgat tatataagta
     2941 aaatgcaaca tatagatgtg aatacatggc ttcaaggcga tatacttcaa aaaattagca
     3001 aactttctac agctgagcag gttgaactta gagtaccatt tttatataaa gatgtatttg
     3061 atgtcgcaaa aaatttaaga atggagcaaa aaataaataa gaataataca aaagttttac
     3121 ttagagaggc gtttagagaa atagtcccag aacatgttgt tcaaaggaaa aaattaggat
     3181 ttccaactcc aataagagtt tggttaaaag attctctagg ggatattgta aaggaaacta
     3241 tttataattc aaatgtagat gaatttatag ataaaaaata tgcaataaaa cttttagata
     3301 cgcatcttaa aggtcataga gataattcga gaaaaatatg gactatattt acattttgtc
     3361 tatggcatca attatttatt gagcataaaa atgtcgagta ttaatacttt tttcaatatt
     3421 aatcagaggg caaaagcatg caatgttttc accctcttga ttattatgga ataggataag
     3481 tagaattttg attgattagt ttactatctc aataaaatta ttggcaaata ttttgacaag
     3541 actactttca acagctaaat ttaagttttg tccatcatca tatccagcgg aaatcaatcc
     3601 tagtaagttt ccaaacatat ctaaaagagc tcctcctgaa ctaccactag agattggagc
     3661 tgtaaattgt atcatttgaa ccgtttcaaa atcacgaaaa gctgaaataa ttccatctga
     3721 aactgtatta aaaagaccta gagggcttcc aattgcaact attttttgac ctctcacagg
     3781 ttttttactg actttaacag gtattggctt gcattttcta tcaaccttta tcacagctaa
     3841 atcataatct gaatggtatt ttataatttg gtaagatgta taaactttat catcattttc
     3901 aaatctaact aaaaatgaat acccatcatt gactacatga agattcgtaa gtatataacc
     3961 ttcattattt attactactc cagaaccgct cttaaacggt ttattttcgt tattgcacac
     4021 aacaatcata acaacagaag ctgataactt tgcaaggtct tcataagaaa gttctacttc
     4081 ctcatcttgt acaatttttt tagatgttag atttgtgtta ttaagtgtat ttttagagtt
     4141 aggtttattc gtagtaggag aagtcttatt ggtattttca ctgtaattta tatttgtaat
     4201 atgcctttca aaaggtattt ctaatatatt tggagtcttc ctagaaagtt caaatagagt
     4261 agaaaatttt gaacattcag gatataaatc aagaatatct ttatttggat ttatatataa
     4321 aatatcacac ccaagttgtg ataaaaaata taagaaaaaa agttcttgcc ttttaatatt
     4381 tccaaagtat actatctttg gtgaaatagt agtcttcacc ttgttataaa ataatttagg
     4441 tagaaaatag tctatccaac acaaaagttt aactgcaaag tttttgatga tagaggatgt
     4501 acaatttggc atacttgtgg aaaataaatc taaagtattt ttaaatgcaa tctttttagt
     4561 ccaatttaag gtgtcatttt ctattttaca tggaaagtcg atattatata aatcccaact
     4621 gttaaactta gaactattat aaatattaaa aattttaagc atcttatctg tttgttcttt
     4681 gtcaaaattt atttttagat tgtttaatct aatataggga attgtaggat tattaaaaaa
     4741 ataagtatct atatttttaa ttttttctat gtatttatca attgaatcat caacacctat
     4801 atatcttgta aagtagactt ctttaatata tgaagaactc ttatttttat aggtagtatc
     4861 agatataaat tcaattagag gttcagtaga cttttttatt gaaatcttta atggtttaat
     4921 agtacaaatc atattttttc tcctataata agatgatata atctattata aaacaactta
     4981 aaacaatata gtacaataat tttgtagttt caaaataaaa tatgtacttg tttatataaa
     5041 tgatatataa ttacaataaa attgccgaaa agccctctcc tttaggtgta gggatgaaag
     5101 gtaaggtctt aatataaaaa aagttagaat aatgcttgta atctaatacc gcta
//
LOCUS       DAMJYX010000174         5136 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJYX010000174 DAMJYX010000000
VERSION     DAMJYX010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.179"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(149..349)
                     /locus_tag="PC479_003395"
     CDS             complement(149..349)
                     /locus_tag="PC479_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2805909.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(574..750)
                     /locus_tag="PC479_003396"
     CDS             complement(574..750)
                     /locus_tag="PC479_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805910.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(804..893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1073..1273)
                     /locus_tag="PC479_003397"
     CDS             complement(1073..1273)
                     /locus_tag="PC479_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2805911.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1275..1625)
                     /locus_tag="PC479_003398"
     CDS             complement(1275..1625)
                     /locus_tag="PC479_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2805912.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1787..1975
                     /locus_tag="PC479_003399"
     CDS             1787..1975
                     /locus_tag="PC479_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2805913.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(2452..2622)
                     /locus_tag="PC479_003400"
     CDS             complement(2452..2622)
                     /locus_tag="PC479_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805914.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESRYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3011..3499)
                     /locus_tag="PC479_003401"
     CDS             complement(3011..3499)
                     /locus_tag="PC479_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2805915.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            4303..4677
                     /locus_tag="PC479_003402"
     CDS             4303..4677
                     /locus_tag="PC479_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2805916.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            4782..5136
                     /locus_tag="PC479_003403"
                     /pseudo
     CDS             4782..5136
                     /locus_tag="PC479_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2067 a          625 c          586 g         1858 t
ORIGIN      
        1 tatatttttg ttagatttat tttataaaaa gtatttagac taagtattat ttttaagtat
       61 tttcttgttg aagttatttt ttcctttttt acaaaattcg taaataaaaa aaagactctt
      121 aaaagagtct ttccgaaaaa tctatatatt ataatatagt tatattttct gcttgaggac
      181 ctctagcgcc tttaactata tcaaagctta ctttttgacc ttcttctaat gatttaaatc
      241 ctgaagtttg tatagctgag aaatgagcaa atacgtcatc tccactttcc attgatataa
      301 atccaaatcc tttttcatta ttaaaccatt ttactattcc attattcatt aaaaatactc
      361 ctattaacct atatcaagat atacttgatt tacttgtgta ttaccacaaa tattattgta
      421 tcatagatta acataaatgt ctatttaatt ataattttta gttaacattt ttatttattc
      481 ttaatatgaa aataaattta aacattttat attaccaata tattaatgta aatgtagata
      541 aaactaaaaa gggttttcta gaaaaaaatc tgtctaatta tacttactta ccaataccat
      601 cacttgatgc aagcaagatt ccacaacttt ttctccgtac tctaatataa aatcatctaa
      661 ctgtgtatct ataaaatcac aataatcgta tgaattacta atataagcag ttaacatatg
      721 tgtacattta tccattttat ctctaaacat aatgtattta tccccctaaa aaactttcat
      781 gaaaaacaaa aagaaaatat ctttttggca actggctgac ataacttatt ttataagcgt
      841 tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag ttttattcta
      901 cagattaata atacaataga attagttatc attcaacata tctagttgaa taattaataa
      961 atttatatat gaactttaaa ttatttgaaa aaataaaaag cccctctgtc aggagtgact
     1021 taaaaattga atttaagttt gtatttaaaa cacattttta ataatgcaaa aactaatcaa
     1081 aaataaacaa ttcatcaata ggtgtatcta ctatttttga tatatctatt gctaatttca
     1141 aagaaggatt atactttcct gcttctaaac gcataatggt ttctcttcta actcctactt
     1201 tttctgctaa ttgttcttga gttaattctt ttaattgtct atattttttt aggttacaag
     1261 tgaatttagg catattattt ttcctctaaa atataaagtt ttatagcata aacatttaaa
     1321 gtgattgcat atgtaattga tagtaaaaca cacattaatt ctatattcct agacactata
     1381 gtaatacacc ataaaatgaa taattctata atagcaagct gtcctgtaaa tgccatagct
     1441 gctttttcat cttgtacata cctttcatct gctttatcac catttatttt tgctataata
     1501 aagtttgaga aaaaagcaaa aaatccaata tatgtaaggt cagcaacatt gccagatgaa
     1561 aaatatctaa gacttaaaaa tccaaggaaa ccacaaaaac ccataaaaaa ttttttctga
     1621 atcatttttt atcctcctta gtaattttaa aagagatata tttatatctt tatgatacaa
     1681 atatatctct tttatattct tttgtcaata gaaaatcagt attatacaat atttatactg
     1741 caccataatt gttagagcaa aaatctaatg cttgagatgt ttatgtatga ctaaatatga
     1801 ttttaatttc aaattaaaag tagctaaatt ttatttgaat agagaaggaa gagatatttt
     1861 aatagtaaaa caatatggag tttatgacca ttctcaagtt gaaagatggg ttaatatatt
     1921 aggagagagt gaactaaaaa taaaaaaatt ttctatacta tatggttcaa gttagatgtt
     1981 ataaactata tgatgataac taaatatagt gtacaataaa tcgctaagta ttttggaata
     2041 aacaatagca cagttatagc aacatgaaaa taaaaatttt tacatagtgg tgttgatgcc
     2101 ttttctaaaa tgaaaggaag accataatga ataaaaacaa aaacactaaa gaaaaaataa
     2161 attaacaaga gaacaagaat tagaatatga aaataaattt ttaaaagatg agcttgagtt
     2221 tttaaaaaag atgtgtgtct cagggaagcc acattaaaaa acagtggtat cattattaat
     2281 caaaagaaag ttagatgtat tatcaaaaaa ttagaactta aatgcatagc attttttcat
     2341 aaatcacata aatataactc ctataaaggt ataattggaa aagtaaaaaa taataaaaca
     2401 aatagaagat ttaaaacatc aatagcatat cagaaaataa caaatgatat gctagaattt
     2461 ataaaatata tctaatgata aaaatggaaa atcaattata aaaaaggcat atctcgattc
     2521 attttttgtt atatagtcaa ataagaatac tatagatgaa agatacgatt ttgcatatgt
     2581 attaagtaag tattctgatg tttttggttg atacacatgc aataaagaag ttgtgtttgc
     2641 agataaatta gcaaaactat agtataagtt gcaacaataa agaaaatgaa aattatcata
     2701 ctaaagcata aggcatgtta caattttatt agataggaca ttaacataca ctaaagtact
     2761 ttttattata aagagtactt atttttttga aattcattaa tgtataaact atcaagaata
     2821 ttactcaaca taatttaaaa acaacttgaa taggtatttt taaattatgt gatataataa
     2881 aaaatagaag tgtatgccaa tacacttctg tagttataac taagcattct attcttgagg
     2941 agtgcttttt tcatttttat aaccaactct ttttacttta acaataatta tcagacctat
     3001 atatttattt ttaattgtct gatttattag ataaaaaagt tattatcata tttttatcat
     3061 ttggtatttt tattgaacta ccataatctt cttctaattt atctagacca cttttactta
     3121 tttctccatc tataattatg tttaattcta agtattcttc actaccatta gatgacacta
     3181 aagtgtacga accattataa ggcatgccta aattttctat tttatgtgcc aaagggtctt
     3241 catcaccaat accagagttt actgttattt ttccatcttt tttagtccac catattatgt
     3301 ttccttcagt atattttgat tcatctgcat agtagctaag agacgcactt tttattgcac
     3361 ttatatcagc ttcaagttta gctatctttg ctttttctat atttttaaat aatgctggaa
     3421 gtgctactat tgccaatatt cctattatag caattactac taataattcc actagagtaa
     3481 atcctttttt atttttcatt tatttcccct ccatataaaa ttaataatat ataaattttt
     3541 attttgttaa tttctacttt ctatatttta ccatatatag acaaaaaaat cactttctat
     3601 gacaaatata ctattatttt acaatttatg gaaatgattt aaaatgacat ttcttgttag
     3661 aaaagatata agaaatggat taaaataaag aaaactacag tcatatcaat gtcaataata
     3721 ttatttaaaa gcagatgaaa ataaaaagag gactcagaaa ataatgtcta tttaattata
     3781 cttacttacc aataccataa cttgatataa acaagattat actacatttt tttatgctct
     3841 agtataaaat catctaactg tgtatctata aaatcaaaat aattatatga actatcagta
     3901 taataagtca aaatatgtat acatgaatac atattttaaa agatatatat tattacatac
     3961 ctaaaaaact ttcataaaaa caaaaataaa atatgctttt agtaacttgt taacataact
     4021 tataatttat tttataagca ttagcctata tagctttgtg ctactaaatc tctttgattc
     4081 taacgattta gtgctattct acaactaaaa taatacaata gagctattta ttgtttggta
     4141 gcattgtttg taaattaaac aaatttatac atatgaatat tattttacat tcaaaaaaac
     4201 agactaaata tgtagaatat ttggtaaaac ttacctctag tttttgtctt aaaaagctcc
     4261 tattattaac ttaagttaga agaattaagg aagtgacata aattgaaaga agaaatatta
     4321 atagaaaaat tattaagagc agaattaata gtaatggagt atttatggaa aaaagacact
     4381 ctaatgccta aaaaagaaat tgtaaaagaa ataaaacaga tttatggatg gcataaaagt
     4441 acaataaaaa tactactaaa aaggctagtt gctaaaaggt atttggctag atatattata
     4501 aattctcaat ctcattataa aataatagat agtaaagaat attatacttt taagaaaaag
     4561 gtactaaaat ctagtaaaag cataaaaata atgcgttcac ttaccactac acataaaaat
     4621 gtaagtaaag agaagttaga cctattagat gagtattata gaaacttaaa agaatgatat
     4681 agtaaaaatc gaatgtaatt atttacctta attcttttgt ttgtaaaact ttattgatac
     4741 agaatttaac atatacataa attggtaaag gaatgtatac tatgaaaatt agtaaattac
     4801 cagaagcgga attaaaagtt atgagatata tttgggaatc agaaaaggta ttaatatcaa
     4861 gagaaatcac caagtctatg gaacaaaaat atgaatggaa agatacaact atatttacag
     4921 tattaaaaag attactaaag aagagagttt ttagaagcgg agaaaataga taagtgtaca
     4981 cactataatg tattagtaaa aaaaaataat atcagagatt ttaaacaaaa gaatttttaa
     5041 agaatacaca taaaaattca atattaagtt taatgtcagc actatgtagt aaggatgatg
     5101 aagtagatgg tgatatttta aatttattag aagaat
//
LOCUS       DAMJYX010000175         5094 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJYX010000175 DAMJYX010000000
VERSION     DAMJYX010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.180"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(27..215)
                     /locus_tag="PC479_003404"
     CDS             complement(27..215)
                     /locus_tag="PC479_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805917.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            550..1518
                     /locus_tag="PC479_003405"
     CDS             550..1518
                     /locus_tag="PC479_003405"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2805918.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1635..2765)
                     /locus_tag="PC479_003406"
     CDS             complement(1635..2765)
                     /locus_tag="PC479_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2805919.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIY
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2918..3871)
                     /gene="msrA"
                     /locus_tag="PC479_003407"
     CDS             complement(2918..3871)
                     /gene="msrA"
                     /locus_tag="PC479_003407"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2805920.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3995..4900)
                     /locus_tag="PC479_003408"
     CDS             complement(3995..4900)
                     /locus_tag="PC479_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2805921.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
BASE COUNT         1719 a          827 c          563 g         1985 t
ORIGIN      
        1 aaacatgtta aattgcaaaa taagcttcaa tctcttttac taatttcatc cattattaat
       61 gattttgaaa tatacattac ttctataata ttcttaaaac gtgtatgatg agatgtctcc
      121 tcagaaaatt tcttctgtcc ttttccacaa ttaataatac ttgaagatac tactctcagt
      181 gcttccacca attcttcttt tgtatgataa ttcataacac atttactcct ttatttttta
      241 atttagagaa acagtatatc tcaatttaat atcattataa actgacatca ttattattta
      301 tacacatctt ttagcactta aatattctaa ctatcagtga cattacatat tttaataatt
      361 attaatatac atataatact ttaatattca ctaatagaga gtatattaac attgcttttt
      421 taaaaatttt agtataaaat catattaaat aaattttctg aaataataga atattaaaat
      481 atccaaaaac agcttttaat aacatctata attttcaatg tatcgttatt ttagggggag
      541 tgatttaaaa tggcaataaa gccaagaaaa atatctattg taggttcagg gcatgttggt
      601 tctcattgtg gatttagttt aataactcaa ggagtttgtg atgaactctt tatgattgat
      661 atagatgaat ctaaatcaaa agctcaagct ttagatttag ctgatgcagt ttcatatctg
      721 ccacataaag tacatataga aaaaggaact ttttctgact gtaaagattc tgacatagta
      781 gtgataagtg ttgcagactc ttcagaaggg cctttacgta gacaaaacac aactagatta
      841 gatttactaa gaccaactat tggaatgata aaatctatag taaaacccat agttgattct
      901 ggctttgatg gtatatttgt agtcatatct aatcctgttg atgtagttac aaattatatt
      961 tgggaaaaat ctggctttcc taaaaacaaa gtcattggaa ctggaacagc tttagattca
     1021 acaagactta gaaggatttt atctgaagaa actggaatcg ctcaacaatc aattcaagct
     1081 tattctatgg gtgaacatgg agattctcaa atggttcctt ggtctcatgt ttcaattggt
     1141 ggtaaaccaa tcttagacat gataaaagac aacccaagta cttatagcaa tttagactta
     1201 ccatcaatag ttgaaaaaaa taaaaaaaca gggataagta taataaatgg taaagattgt
     1261 acagaatttg gtataggtac tgctttagta gaaattgtaa aagctatact acacaatgag
     1321 aaaaaagttc ttccagtttc tacattacta gaaggtcaat ataacgaaag aaatgtattt
     1381 gcaggtgttc catgtgttat aggaaaagat ggtattgaag aaattattga aatcaatatg
     1441 actgaatatg agcaaaatga atttaataaa tcttgttctg ttttaaggga atatattgca
     1501 ctttcaaaaa cactttagat tcaatattct ttcttacact aaaagcaggt aaatgactta
     1561 ttgaaaagac ctttaactaa gttaatgttt aaatcatttg tacctgcttt taattttaat
     1621 actaaaatat aaacctattt tttaccatca aattttataa ctttactttt tttatttacc
     1681 tcttgagata acaatccttt tttcaatccc atcaataatt ctaagtcttc tggataaaga
     1741 tttattccaa attcctctaa acttgatact atactattta cactcattat tttattttct
     1801 atcaataatt caactgctct ttttaaatac gttgggcttg aacctttcat tttgtctagt
     1861 ggttcttcta tcaaccagcc ttttttatcc atttcattca taagataatt gtattttttg
     1921 taatttatct tttctagatt atatgctcta aaaatcatag cagatatagg tacaatccat
     1981 tttttcttta gttctacata ataatctaaa tcttctggat gagtcaaatc ttttaaaaaa
     2041 ctctcttttg gcaataaaaa tgcacaggca tactcatctt ctgaaaattg cttacttggt
     2101 attcttaatt cacttgatat tatatatcca agctcacatg ccaaagtata atttctaatt
     2161 gatgctgact ttttgtcatt acccagagat attagatatt tagattcttt actaattctc
     2221 tgtttttgag taaagatagt tgcccctttt ctatctacat tcatacctga tattataatc
     2281 cctttagact ctaacaaact taccatattt actattggag tactatttaa atcccaaaac
     2341 tctctagtct tatatgcaag agattctata tcatttatat taatatctga cattactttt
     2401 ctatttggca agttttcttt tggaaactta atataattct ccataaatga atatatttta
     2461 tatattataa ttattttttc tctataagaa atctcttcta ctctaggtag tttagattga
     2521 gggttaaagt ggctatcctc tacaacgata tttatatttt catttttata aaaatactct
     2581 tttggaaaat ttaatatatt agataatttt aatgtatttt ctatatttgg tacatactta
     2641 ttttcctcaa aagcttttaa atctttttta tttattttag tttctttaga caactgttct
     2701 atcgtctttg ctctaaaaat tctagctgat tttaatctct ttccattaaa gtcttttaaa
     2761 ttcatttgtt ttctctcttt caaaattaaa ttcttaatct attggtataa ataagattac
     2821 aattaataat ttatcatatt attgacaaaa aaacaaaagg tgaaaatatt tttttatatt
     2881 ttcacctttt agataagttt ataataaata ttcctaatta ctcaaaaagt tttaaatatt
     2941 ctccatatcc taaagcttcc atattttctc ttggtataaa tttaagagaa gcagaattta
     3001 tacaatatct taaacctcca agttcttttg gaccatcatc aaatacatga cctaaatgag
     3061 aatttccttt agcacttctt acttcaactc tgtacatacc ttgtgagaaa tcacttctct
     3121 caatgataga tttatctata actggctttg aaaaacttgg ccacccacat ccagaatcaa
     3181 atttatcttt tgaagaaaat aacacttctc cacttacaat atcaacataa attccctctt
     3241 tgttattgtc ataatattca ttttcaaaag gtacttctgt catatcattt tgagtgactt
     3301 caaattgtat aggagttaga gtctttttta actcttccct atttatattg tttccatccc
     3361 aatggctttt tacaaaatta tatcttccag accctttata atacatattg tagtttaatg
     3421 gtcttttctt ataataatcc tgatgatttt cttctgcttc gtagaattta gttgctggaa
     3481 ttatatcagt cactatttta ctatcaaata ttttactatt ttccagatgt tctcttgact
     3541 ctaaagctat atttttttgt tcttcatcat gataaaatat agcagtctta tattgagttc
     3601 ccctatcacc aaattgtcca ccatcatctg taggattaat ctgtttccag aagatattta
     3661 gaagttcatc ataacttatt atatcatcat catatgttat ttgaattgct tctaaatgac
     3721 cagtagttcc tgagcatacc tcttgatatg tagggttttc aacatgacca cctgtatatc
     3781 cagaaactac tttaataact ccatcatact tatcaaaagg ttttaccata caccaaaaac
     3841 atccacctga aaaagtagca agttttatca ttttcatcac tccttaaata aattatactt
     3901 tatttattcc ctaagaagta acttaataat ctgcatttta ttatttttac ctactcaatg
     3961 aaatcatgtg taaaaataca attgcaaatt gaatctaatt tctataaaag ctctttttct
     4021 ttatccatcc aaaagcatct tttatatcac aattttctcc taaagacatt agatattcta
     4081 atgcatcaca atcatccata gctacatctt tttccatctt aggatttaat ctatagtaat
     4141 tatctccaag aagtttttga gaaaataact gactcatttg agaatttcct tctaaggtaa
     4201 tagatataat tggtaaatcg ggttctttat taattatcca gtcaatagct ccccattcgg
     4261 tagtatctgc ctttatactg ttatatacat atccagtccc tattgaaaaa agtcttgtat
     4321 tcttaagttt aaatccactg tcaatggcat gaattataca cgctaaactc gggtcagttg
     4381 ctattattcc tccatcaaca tgacgattat atgttggaaa aaataccgga gcagcactac
     4441 ttgccattgc tacatctaca actttgctag tctttgtaaa agaatttggc atattgttat
     4501 aaaacactgg tttccatgca ttttcctcat ttcctatata aaaacttggt atcattacta
     4561 atttatttaa atcttttaat tctagttcct ctggaaatat acttaataaa acttctttta
     4621 agtttttgtt ttcatacttt ggtctgagta tttctgcata acttttatta aaaatatact
     4681 ttgaattttc aattgagtaa agttctttta tctctttagg gcttacacca tacgcaagcc
     4741 ctagtgcaat tagactccct gtagatgtac ctccaaacat atttatattg tttaatagat
     4801 ttggatactc ttttactatc ctctcaagta tggatatact caatgcccct tttaatccac
     4861 caccatcaaa tgccaatata ttaaaattag aatttttcat atacatatac ccccacataa
     4921 aacaccttat attttaaatt ttaatcaaaa taatttcact atagtatata tattttatgc
     4981 aagttacata ttacaatgtt cctcttttaa tatttaagtt acattcaaac ctttaatact
     5041 taaaataaaa tatataagat attatatatc taagtattat aaatttaata caaa
//
LOCUS       DAMJYX010000176         5023 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJYX010000176 DAMJYX010000000
VERSION     DAMJYX010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.181"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            14..1897
                     /locus_tag="PC479_003409"
     CDS             14..1897
                     /locus_tag="PC479_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2805922.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2121..2801
                     /gene="pgmB"
                     /locus_tag="PC479_003410"
     CDS             2121..2801
                     /gene="pgmB"
                     /locus_tag="PC479_003410"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2805923.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2798..>5023
                     /locus_tag="PC479_003411"
     CDS             2798..>5023
                     /locus_tag="PC479_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2805924.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PI"
BASE COUNT         2072 a          456 c          954 g         1541 t
ORIGIN      
        1 gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta atcaaatcaa
       61 aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa tgagcaatta
      121 tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa aaaatgaagc
      181 gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag gtgcaggagg
      241 aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag taatgagtaa
      301 agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt taaataaacg
      361 ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta cagaaaaaag
      421 aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag gtaatttttt
      481 tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg tgaaagatac
      541 tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta taaacccaga
      601 aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg gagatttaca
      661 aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa tttacttttg
      721 ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata tggaggtaga
      781 ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc ataagagaaa
      841 tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta aacagtggca
      901 agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata taaaagatat
      961 gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt atgaaacctt
     1021 tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa taaaatattt
     1081 attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga gattagatgt
     1141 ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca aaaaagtaaa
     1201 accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggt taatgggtga
     1261 ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat tcttttgtac
     1321 agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg taagttatcc
     1381 aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta caagaatatt
     1441 gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta tgtttgtaca
     1501 aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag aattaagttc
     1561 aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga ataaggatat
     1621 atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac ttaggtctat
     1681 atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga aaaaagaatc
     1741 tatttctata ataataaata attcatctaa aaatataatt ttgacgttac cagattattt
     1801 agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa aagaagaact
     1861 aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat ttattttaga
     1921 tttcatgtta aattattata cagtttaaac aagtctttat aaacgataaa aaatatggtc
     1981 tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag tagataattt
     2041 tataaataat atcttaaata caagatagaa aataaattga ttattagtct aaaaatttct
     2101 tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat ggtgtaatta
     2161 cagatactgc atattatcac tatatggctt ggagaaaact agctcataaa gttgggatag
     2221 atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag tcattggaca
     2281 ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa gtgagaatgg
     2341 ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca aatgatattt
     2401 tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag ataggtctat
     2461 cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt gacaaatttg
     2521 attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag atatttctta
     2581 tgtcagcgaa agggcttaat gtaaatccac aaaattgcat agggatagag gatgcaagtg
     2641 caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga aattatgaaa
     2701 acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt gaatatatac
     2761 aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat ttagtaatgt
     2821 agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat tagcagagtc
     2881 cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg aaaattatag
     2941 tggagataca cacaaggggt cttatatagc tggagtatgg tttccagata aaactcgtgt
     3001 tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt cagtcaacta
     3061 tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat gtaaagtaga
     3121 agattttcat agagaattag atatgaaaaa tggtatttta aatcgtaggt ttattgtaaa
     3181 tataaatgat aaaaaatttg aagttagggt tactaggttt ttaagtatat ctgttaagga
     3241 acttgcagta attaaataca gtataaaatc attaaattct aatactaaaa taaaatttgt
     3301 accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa agttttggaa
     3361 tggaatttct tcaagttctt tagaatataa agggagtatt ctttctcgaa caaaggagaa
     3421 tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga caaatggtga
     3481 tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga taacaatgga
     3541 tgttttaaaa aatgaggttg caagtataga aaaatatgta tctataacct caactagaga
     3601 ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatagag aactatctaa
     3661 aagttatgat gatatattaa atgaacatat aaagttatgg aataaaagat gggaaagtgc
     3721 agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata atttattcca
     3781 actattttca acatattatg gagaagattc gcgattaaat ataggtccaa aaggatttac
     3841 tggtgaaaag tatggaggag ctacttattg ggatacagaa gcgttttgtg ttccagtata
     3901 tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt acaatcagtt
     3961 agaacaggca aaggataatg caaaaaaact aggtcttaaa ggtgctttat atccaatggt
     4021 aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg aaatccaccg
     4081 aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg actataaata
     4141 tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg caagtagagt
     4201 tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc caaacgagta
     4261 tgaaaacaat atcaataata attggtatac taattatata gctaaatggt gtttagatta
     4321 tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg acagaaataa
     4381 tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt atctgctgta
     4441 tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag aatttgtaga
     4501 agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt gggataagat
     4561 tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact attttggaaa
     4621 tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat atactgtaca
     4681 tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag ataacctaga
     4741 aaaggcttat gaattatatt caagaacagc taggcttgat ttagataatt acaataatga
     4801 tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca ttgtacatgg
     4861 atttgcaggc atgagaacat ggaatgaaac tctatctttt gaccctaagt tgccaaaaga
     4921 gtggaaaaat tattctttca atataaatta tagagataat attattaatg tagatgtatg
     4981 tagtgattta attaaaatag aaaatttaaa aggtaaacca ata
//
LOCUS       DAMJYX010000177         4847 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJYX010000177 DAMJYX010000000
VERSION     DAMJYX010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.182"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..982
                     /locus_tag="PC479_003412"
     CDS             <1..982
                     /locus_tag="PC479_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2805925.1"
                     /translation="ISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYKYIL
                     FSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISK
                     DELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDK
                     PLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVK
                     LTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITISDNGI
                     GCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            975..1622
                     /locus_tag="PC479_003413"
     CDS             975..1622
                     /locus_tag="PC479_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2805926.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1751..2686
                     /locus_tag="PC479_003414"
     CDS             1751..2686
                     /locus_tag="PC479_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805927.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2700..3797
                     /locus_tag="PC479_003415"
     CDS             2700..3797
                     /locus_tag="PC479_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805928.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3800..>4847
                     /locus_tag="PC479_003416"
     CDS             3800..>4847
                     /locus_tag="PC479_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805929.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLR"
BASE COUNT         1886 a          555 c          659 g         1747 t
ORIGIN      
        1 tatttcttac tttttagaac ttatattaat ttttatactt tgtaattata caaaaacttt
       61 taattcgatt tactttgttc ctttaatatt agatatatca tttttaataa aggaaaaata
      121 taagtacata ttgttctctt ttgttataat atcttctctt attattagtt tagataaaaa
      181 tatatatttg gcactggagt caagctctat attgctaatt ataactgtac tttctatata
      241 tatagaaaat gagaatttat caaaattata cagtcaaaat ctttatgata agcttagaat
      301 ttctaaagat gaattaaaaa aagttaatgc tgatttggaa acttatgcaa gttctataga
      361 agaattagca attttaaaag aaagaaatcg aatatcaaga gaaatacatg atagtgtagg
      421 acattctctt tctacaacaa taatacaatt aaatgctata gaaaaactct taaaagacaa
      481 acctctaatt tatgacttag tacatgaact tagagaattt gtaactgaaa gttttcaaga
      541 tgtaagaagg gctatatctg agttaaaacc tgttgaatat gaaaattacc aaagtttatt
      601 taaaatcaaa gaattagtta aaagctttat taagcttaca aatattaatg ttaaattaac
      661 tatttcaaaa aatacttgga acttatccag aaatcaatct atagctttat atagacttat
      721 acaggaatcc ctttccaact catcgatgca tggaaaggca actgaaatca gaatttttat
      781 tacattcaat acttcaaatc ttataattac tattagtgat aatggtatag gatgtggcaa
      841 tataaaaaaa ggaaatggac ttaatagtat caatgagaga atatatgaat taaatggtaa
      901 agttgaattt gataattcta ataatggttt cacaattaga gcttcttttc ctaaattttc
      961 agggggtgat ttttttgaat aaaattaaag ttcttatagt agatgacgaa aaattaatta
     1021 gaaaaggtct taaaattata ttatcatctt ataatgattt agaaattgta ggagatgctt
     1081 ctaatggata tgaagcttta gaattttgta aaatgaatga tgtagacata gttcttatgg
     1141 atatacgaat gaaagtatgt gatggggttc taggtacaag gcttataaag gaatataata
     1201 attctataac attattaatt ttaacaactt ttaatgatga tgaatatata aaagatgcta
     1261 tgaagtttgg tgcttcagga taccttttaa aagatagctc tgatgaagta ttgcatgaag
     1321 ggattcgttc ttcatttttt ggcaatattg tgcttgataa aagtgtagct gaaaaaatca
     1381 tgacctctga aaaaacgatt aaacaagagc atctttatga catgtacaat ttaacagaaa
     1441 aagaaatatc tataattaga ctaattgcaa atggacttaa taataaagaa ataagtcaag
     1501 aactattctt atctgaaggt acaataaaga ataacataac taatatttta gctaaactag
     1561 aacttcgtga cagaactcaa ctagcaatct ttgctttcaa aaataaaatt gtaatagaat
     1621 aaatctaaaa atataaagtg acttaagtca ctttttttag tgacttatag taatagaatt
     1681 ataattcaca attctttatt ataatattat agctttttaa cttatacaaa attattttgg
     1741 agggattact atggatatag tcagagtaaa taatataaca aaaagattta atgataaatt
     1801 agtacttgat aacatcagct tttctgttaa aaaaggtgag atatttggac ttattggccc
     1861 aaatggtgca ggtaaatcta cacttataaa tataattaca aatcttatgt taccaaatag
     1921 tggtagtatt caaatcaatg gtttagattt atcaaaagat tatataaaag caaagtctat
     1981 aatagggtta gttccacaag aattagcaat tatagaaact cttacccctt ttgataactt
     2041 agagtacttt ggagctttct acggtttaaa gggaaaacta cttaaagaaa gaatcattga
     2101 agcattagaa gttacaggtc ttactgaagt caaaaagaaa aaggtcaaaa aattatctgg
     2161 tggtatgcaa aggagattaa atattggaat agcactctta aatcatccta aaatattaat
     2221 attggatgaa ccaactgtag gtgtagaccc ccaatcaaga aatcatattt tcaattttat
     2281 aaaagatata tctaaaaaac atgaaactac tgtaatatat acttcccatt atatggaaga
     2341 agttgagcac ctttgttcta aaatttttat tatggatgaa ggtaaagaaa ttgcttttgg
     2401 aaataatgat tatttaaaat cactcgtatc tacaaataca aaattaatta tggaaataaa
     2461 aaatataaat gcccaactta tctttgattt aaaaaatact aaaggtgtta tatctgtttt
     2521 agaaaataat tcattattag aattggatat agataaaaaa ttacaactta cagatatact
     2581 ctctgtcatt gacaaaaaca attcaaaaat aatgaaaatt tcttatgaag aacctagcct
     2641 agaagacgta tttttaaatc taacaggaaa aaatttaagg gactaatgga ggtgatttta
     2701 tgaaattata catgagtgta aaaactatgc taaagagttt aaaaagctcc tttatactta
     2761 atttaatata ttttttagct ttgcctctga ttttatcttg gtttcttggg atggttacag
     2821 aaagcatgtt tcaaaaccct ataaaaacag aatctacccc cattgttata tatgacaaag
     2881 ataacacaag attatcaaat gatttgacaa agtatttgaa aaatgattta tcttatatat
     2941 taacagttaa aaaagatgat tctaaggcag aattaaagct tactatacct aaaggctatg
     3001 agagtagttt attaaatgaa aaatcaaata ctctaaacat agaaaaactt ggaactcgtg
     3061 atgatattgc aatattgctt caagatatat tagatactta ccatgaaaaa ttttatttaa
     3121 ataattctca gaaaataagt tcagaagatt tttctaaatt atttaataaa aattctattg
     3181 atacttctat aataaataac aatgttaaac agagctctta tgaatacttt gctttagttt
     3241 cactaggatt tcttgtaata atttttataa tgaacaatat tttgtcaaat tatataagtg
     3301 aatcaaaggg actttcaaaa agactttatt ctatgcctat aactagagta caattcttaa
     3361 tttatgactt tgtaggttta tggatttatt catttatatt cttattatta tatgttttat
     3421 tctttagaat acttggaata acatttaaag gtaattttgc catattgctc ctactttgtg
     3481 ctttatcatc ttattttatg acttcaatat ctacctttgt aaccagcttt tttagtaaaa
     3541 aatacggtac agcaatagtt tatgcattac tctttttaca gacaattttt ggaggtatct
     3601 tcagtatgat aagtgataca tttactaagc ttacaagttt atctcctacc tatttaatag
     3661 gagaattgtt tagtaattat gaaacattta aaaccataga ttcaatagga aatttaattt
     3721 tcacatgttt aattatctct acaatattaa ttgcacttgc tattgtaaag gaaaaatata
     3781 aatggaggga ggtttaataa tgattagatt aactattgca aatataaaaa gatatctaaa
     3841 aaatcatact cttttaatta atatggttat gcttcctatt atactaattt tttcacttaa
     3901 tttttttatc aacaactcgg gaaatcaatc aatgtacttt tctccagtag caattgtatc
     3961 tgactcttca ggaaagtatg aacataagtt aataaatagt tctaaattaa aagaaaattc
     4021 atttagatta aatgaacaag ataaggctat gaatctatta aaaaataata aggtttctgc
     4081 tgtattcgtt ttagataaaa atttttcaaa tagtatagat aaattacaac gacctgtagt
     4141 taaatgcttt aaaattgaaa atggtggtgg tagtctttgg gcagaatcac aaatagaatc
     4201 ttttattact aaatcgttaa aattgaaagt ggataaaaat atagatgata aattgacaaa
     4261 gacaaatata attgataaca aaccagataa gaataaaggt tcatttttag tagtcttctt
     4321 gatatgctat tttatgtata ttaatgcggc acatcttgct tctgatttat ttacattaaa
     4381 aaaatcaaat gtgctaaaaa gacttatctc tacagataat aaagatataa aaataatttt
     4441 cagtatattt ttaggactat tttttattca atcaattgta tacacttttg ctctattatg
     4501 tttttcaatt atagaagact ttaatttgag tctgaatgta ttgatgattg tactagcaaa
     4561 tagttttgtt tccactggat ttgtattttg ggtagctaga gtatttaaaa atgaaagttc
     4621 tataagtctt atatctacat tttattcttt aataggtcta gctatatcta tctctagtct
     4681 aataccatca atggataaat tatcctttat gactaatctt tcaaagttca caccatttta
     4741 ttggactata gatgctatta aaaacaatgg tagtatttta ataaatataa tagctctaat
     4801 attaattgga attatatttg taactgcagg aagtcttaaa ctaagag
//
LOCUS       DAMJYX010000178         4576 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJYX010000178 DAMJYX010000000
VERSION     DAMJYX010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.183"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            498..1805
                     /locus_tag="PC479_003417"
     CDS             498..1805
                     /locus_tag="PC479_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805930.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1891..2153
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2279..3082
                     /locus_tag="PC479_003418"
     CDS             2279..3082
                     /locus_tag="PC479_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2805931.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3120..3731
                     /locus_tag="PC479_003419"
     CDS             3120..3731
                     /locus_tag="PC479_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2805932.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3763..4434
                     /locus_tag="PC479_003420"
     CDS             3763..4434
                     /locus_tag="PC479_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2805933.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1832 a          423 c          822 g         1499 t
ORIGIN      
        1 actaaaaggg aatatttaca gtttaatggc agttttaaaa taataaatta aagctgtcat
       61 ttttatttaa aaatttttag aaaaagtaca atagtgttaa tgtgtgaatt agataaggtg
      121 tatataatag tttagatgat atgatataaa atatgttgta aattaaatgt tttctttgac
      181 aatatataca caaggatgat aatatctata tgaataaaag atagtgtgta atttgataat
      241 atgtaattat aacattatga tataattaca ataaagtatg taataatctg tagaaatttc
      301 aagctagtta tctaagtttt cggataacta aatctaaacg gaatacataa tgtattaaat
      361 aaatgatgat actaaaatga tactattttt atagaaattt attgaggtat ataattaatg
      421 aaaataatat attaaattat ataaattaaa ttatataata tattgagttt tacataaaag
      481 caaaggtggg aataaaaatg aaagaaattg ctgatgctat aacatcagag tatttaagct
      541 atggtatcaa tgaagatatt agtatctatg aaggtagatt ttgcatatat ttagataaaa
      601 aatatagatg taatgggaaa atttattata aaatgacacc tccaatatct ataagtttta
      661 aagcagacat aggttgtgtt gaagagatag acaatgaaga tgacaattta gctttagatt
      721 atgataatgc tattcttgaa gttcatggat ataaaattat ttctataaca ataaatactt
      781 taagtgaatt tagtgtagaa ggttatatca atgatgactg tataaaatct aagaactcct
      841 atgttgaata tgtagatttt aatataataa acttagataa aatacctgga aaactcatta
      901 aatacaatga taaggtatac gcaggtagaa ttgaatttga tataaatgat tatgttgtaa
      961 ctatagacaa gcgatatgat tatagaaaag aattaaaatc agaattaaag tctaaatctg
     1021 gagcaataat aactcatata ggcaggatta gaagaaaaga tggtagaatt tttagaacaa
     1081 ataatacaat aaatctttta gataggatat caacagcttt gagttttatg tgtggaagat
     1141 atgtagggtt ttgtttggcc aaagggtata gaagtggtaa tgaagtttat agaatatgga
     1201 atgaaaatca aatatctcct tttagatatg taccaacatg gtcagataca ctttcaaatt
     1261 atcataatat ggaaaaatat ataagcctta tgtgtaaaaa gctagaagat ttttattatg
     1321 gttctgctat aaagagtgtt gttgattggt acatagaatc tcttggaagt gctaccatgg
     1381 aaaacaatat aatatctgta caaattgcac ttgaaacttt gtcatatgta attctagtgg
     1441 aacaaaataa gatacttaca gatgaagcat ttgactgtaa tttggcatct aaaaatataa
     1501 gactcttact tgatacatgt aagataccat atggtaaaca tgaattaaat atatttgata
     1561 atataataaa aaataaattt gatgatggag tggatttagt tatttattta agaaatagca
     1621 tagttcatcc aagtagaaag actcatagag ctgtgttaga ggttgaagat atttggaata
     1681 taatttcaat aggaacgaga tatattgaac ttgttttact ctttatttta ggttatagag
     1741 gagaatattc aaacagatta gtagaaagat gttatggtga agtggaagtt gtaccttgga
     1801 actagatttt tatatttatt ttaaaatata tattgacata tattgcctta tgctataaaa
     1861 tcaatatcat aataataatt cagaataaca ttaaatgcta agaaaagaaa tagtaaaagt
     1921 agttaatctt aaacagagag tttccgattg gtgagaggag caatagattt tactttgaac
     1981 acatctttga gcaggactct gaaagagtag aaatacttta ttaggagatt tccggaatcc
     2041 ttgcacccgt tatagtgcta gagtataata attttattgt acttaaagag gttagtgccg
     2101 tgaggcatta atgaacagag gtggaaccac gagagttaac tcccgtcctc tagcagaagc
     2161 tagaggcggg agttttttaa tataattttt taagcgcgca gagaattata ttgcaataga
     2221 tacacttttt aaaaaataat caaaatattt taaaaattaa aagacgaggg agaatattat
     2281 gaaaaatata ttaaaaaaag taggaatatt tacaattatg ttaggtttat taggtggagt
     2341 tgtaggttgt tcaaaaccag ataatgaaaa agataaggat gcttcaaagg aatctaaaaa
     2401 agaagtagtt gtagggtttg ataatacgtt tgtaccaatg ggatttttag atgaaaaggg
     2461 agatacagta ggctttgacg tagacttagc taaagaaaca tttaaaagac ttggaatgga
     2521 agttaagttt cagcctatag attggtcaat gaaggaaact gaattaaatg attcaaaaac
     2581 tgtagatgta ttatggaatg gatattctat aacagacgaa agaaagaaaa tagtatcata
     2641 tacagaacca tatttacaaa ataaacagat aatagttact ctaagtgact caaaaataaa
     2701 ttcaaaagct gatttaaaag ataaagaagt tggaacacaa caaggttcaa cagcacttga
     2761 tgcagtggaa aaggataaag attttatgaa tagcttaaaa ggtggagcac cagttcttta
     2821 tgatacttat gataaagcac ttagagattt agagataggt agaactagtg cagttgttgg
     2881 tgatgaagtt ttaattagat actatatggg acaaaagggt gaagacaaat acaaggtatt
     2941 aaaagacgat tttggattag aagattatgt tgtagcaact tctaaagaaa atccagaact
     3001 ttgtgagaaa ataaatgaaa cattaaaaga aatgaaaaaa gatggaactt ttgacaagat
     3061 ttatgataag tggtttaaat agaagataag ttagctgtta agaagggggc actaaaggca
     3121 tgttacaagg tttagagata gttatagcaa tgttttgtat aacattgata gtatcaattc
     3181 cattggggat tggagttgca tttttaagat tatcaaagaa taaattagta agtggtatta
     3241 cacaatgtta tatattgata atgagaggta cacctttact tttgcaaatg atagtcatat
     3301 tttacggatt acctctttta ggaattgtat ttgacagatt tacagctgga gtagtagctt
     3361 tcttcttaaa ttatgcagca tattttgcag aaatatttag aggtggaata cagtcaatcg
     3421 atagaggaca atatgaagct tcaaaagtac taggatttga taagtttact atgtataaga
     3481 gagtaatctt tccacaagtt tttaaaagaa tactagcacc tatttcaaat gaagtaatca
     3541 cattagtaaa agatacatct ttagtatata tattaggatt aaatgatatt ttaagaatat
     3601 ctcaaatagc aatgaacagg gaagcaagtc tgttaccatt atttgaggca ggagcgatat
     3661 atcttatatt tgtagctata cttactaagg gatttgaatt acttgaaaaa aaatattctt
     3721 attatagata aagttacaat attataggag gaggtttttt atatgttgaa aatcaaaaac
     3781 ttaaataaat catttaaaaa aaatagagta cttaaagata tttcttttga gttagaagaa
     3841 ggtcagattg gagttttact tggtaagtct ggtgcaggaa agaccacaat attaaggtgt
     3901 ataaatggtc ttgaagaatt tgatagtgga gagattataa tagataatga ggtaataaaa
     3961 aataagagag atatggcaaa aatacgagga aaaataggta tggtttttca aaatttcaac
     4021 ttatttcctc atatgacagt ccttgaaaat ataattgaat ccccagttaa tgtttttaaa
     4081 gttcctagaa aagaggctga agagagggct agagaacttt taagattagt tgatttagaa
     4141 gataaattaa attcttatcc atttgaattg tcaggaggac agcaacagag agttgctata
     4201 gctaggtcat gtgcattgat gccaaaagtc ctttgttttg atgaacctac ttctgcactt
     4261 gatattgata ctattcaaag agtagtcaat ataatgaaca gacttaaaga caaaggtatg
     4321 acaatattaa taattacaca tgatgttgtc ttctcaaata atgttgcaga taaaattata
     4381 agtataaaag atggtatagt agaaaatgtt caaataaaag aaaaaattgt ttagagtttt
     4441 ataaatataa actagggtag aattttataa tataaataaa gggcaagtaa aatcaaatat
     4501 actatgccct tttgttatct caaaatttaa tcttatattt tatgtttaag ttacattata
     4561 atttataata aaaata
//
LOCUS       DAMJYX010000179         4534 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJYX010000179 DAMJYX010000000
VERSION     DAMJYX010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.184"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            144..677
                     /locus_tag="PC479_003421"
     CDS             144..677
                     /locus_tag="PC479_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDF2805934.1"
                     /translation="MTEVIIVNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVD
                     NKMTAQDLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDK
                     EISNQELSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYR
                     MSRALKKLELYLEGGKL"
     gene            674..1435
                     /locus_tag="PC479_003422"
     CDS             674..1435
                     /locus_tag="PC479_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805935.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1473..2339
                     /locus_tag="PC479_003423"
     CDS             1473..2339
                     /locus_tag="PC479_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805936.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2360..3601
                     /locus_tag="PC479_003424"
     CDS             2360..3601
                     /locus_tag="PC479_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2805937.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQILNVLPSNAISFSFTNYVDYKFGNLIIALPTMIIFTC
                     LVLTVVFLPFIKNNFKKHQVN"
     gene            complement(3825..4397)
                     /locus_tag="PC479_003425"
     CDS             complement(3825..4397)
                     /locus_tag="PC479_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2805938.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT         1762 a          517 c          647 g         1608 t
ORIGIN      
        1 tataataaaa ttgtttaagt attaattatg agtattaatt aatggtatag ataattttaa
       61 tataactgta tttataaata attataaaat aataaattaa tttttttata ctgacttttt
      121 tatagtataa atcgttatat ataatgacgg aggtgataat agtgaatgat gctcggatta
      181 tatcacaaat aaaagcagga aaaaaagaat tgtttgatac tctaataaaa aaatactatg
      241 atagcatcta ctgttattgt tatcgccatg tagacaataa aatgactgca caagatttaa
      301 ctcaagatgt atttgtaaca gttataaata atatagaaaa ttataaacat tatggtaagt
      361 ttgaaaacta tttgtatgtt attgcaaaaa acaagtgtaa ggatttttgg aagaaaaaac
      421 aaccaatata tttagagtca ttagataagg agatatcaaa tcaagaatta tctgatatag
      481 aaaataaaat gtttataaaa gaagctttga ctaaattacc agaattagaa tttgaagtga
      541 taattttacg gtattaccat gatttaagat ttaaggatat tgctaaaatt atggagtcaa
      601 gcgtctctgt cacaaagtac aggatgagta gggcattaaa aaaactagaa ttatatctgg
      661 aaggaggtaa attatgaata ataaagaaat aaaaagaagt ctacagaagt ataaggttcc
      721 caaatatgat tcttcagctc ttgaaaagac tattaaaact atacatgagt taacgcttag
      781 taatacttat aaaagaatga ataataaaga cttttttata tctcaaattg gttttataag
      841 aaaaagaact tggattatgc aatttctaat actatgtaca atatgtatat ttttatttag
      901 tacatcagca gatagtaatt cattttataa tagtttatcc ttgatttcta tagcatcacc
      961 actttttgta ctgacaaata tagaagaatt aacaaaagtg tataatagta gtattttaga
     1021 gatagaattt tcaacaaaaa atacattaca taaaattatt gctactagaa tgcttatatt
     1081 tggaataagt gatttattgt ttatgattat tattttggta tctgcaaaac aaatcattga
     1141 tgtaaacatt ctgtatataa taatttatat actagttcct tttaacctaa tgtgtatagg
     1201 atgtatggaa attataaata gaaataaagg taagcataca aattattatt gtatgatata
     1261 cggtatatct cttgtattat taatagttac actgagtaac tttagagata atctatatca
     1321 atttgaagca ataaatcaat ggctaattgt ttttctagtc acagcagtaa cattaatata
     1381 tgaaataaga aaaacgtgga gacatctttg ttcatttgaa aatattatta tctaagtaaa
     1441 aaataagaat acacagtatc aggaggaata gtatggagtt aagtgtagaa cgtttaacaa
     1501 aacagtataa caataaaatt atagtagata aaattgactt taaattaaat gagggtatat
     1561 atggattttt aggagaaaat ggagctggaa aaacaactct tatgagaatg atttgcggaa
     1621 tattaagtcc tacatctgga gaagtaaagg tggatggtaa aaataatttg gatatgggag
     1681 aagattttag agatatgatt ggataccttc ctcaaaactt tggatactac ccagatttta
     1741 ctgccaaaga atttatgcta tatatagcat cattaaaagg attaatgcct agctatgcaa
     1801 agagaaaaac acaagaatta ttagaaatgg taggattatc agaggtaatt aataaaaaaa
     1861 taagaacatt ttctggaggt atgaagcaaa ggctaggatt tgctcaaaca ctacttaatg
     1921 accccaaaat tttaatatta gatgagccaa cagcagggct agaccctaaa gaacgagtta
     1981 gatttcgcaa cctaatttca gaattttcta aggaaaagat tgttatattg tctactcata
     2041 ttgtgtctga tgtagaatat attgcagata aaattttaat aatgaaaaaa ggaagatttg
     2101 ttttaactgg ctcaatagaa gaagtaactt caaagataaa agataaggta tggaaatgtg
     2161 tggtcaatga gaatgaaata gattatatgt ctgatagata ttgtttagca aatatccatc
     2221 attctggtgg agttgcaaat cttagaattg taagtgatat aaagccacat gacaatgcat
     2281 gcaatttaaa accaacatta gaagatttat atttatattg tttcaacgaa gtactataaa
     2341 tatacataag gagataggta tgtctacatt aattaaattt gagttaaaaa aaataataaa
     2401 aagaaagtct acaatagcag ttattttagg aagtttattc ttaagcatca ttctttttgc
     2461 attaatgcca attcaggaaa aagctaatga tgaaagtgga aaagagtata ggggtttaca
     2521 ggctattgag ctaaaaaagc aacaatctaa attaaatgag ggatacataa cagataaaaa
     2581 agtaaaaaac tcaatcagag attaccaaaa aatgtttaat aatccaaata atattacaac
     2641 agatgaaggt gggttttcaa attttaaacc agatatttat tggaaattct tctatccaaa
     2701 taaagctttt tataatttta taatagagtc atatagtaat ccaaatgatt ataatccaga
     2761 aataattaaa tcaatgcctt tagaagatgg agttaatttt tataaattta gaaatgaaaa
     2821 agtaaataca atattaaata tggaatatcc tgataaaaat tacacacaga aagagaaaga
     2881 tttttggttg tctaaaaata aggatataga agagccttat ttaaatggat attatcaagg
     2941 atggaattat tttctattgg ctagagattt actaattttt actatagtag ctgtctgcat
     3001 aagtacagct tctgtatttt caggagaata ccaatccggt gctgattcaa taatattatc
     3061 ttctaaatat ggaaaaacaa aagttataac agctaaaata gtaagttcct ttttatttgg
     3121 tacgattatt tttacaataa atacaattgt gtctattggt atacttcttt tatcttttgg
     3181 aattgatgga tggaatttac cattacaact tacaaccagt tcaattccat acccatatac
     3241 atttttacag gcaatattac taagtgtgtt aattatttat atgatattaa tactattaat
     3301 tagttttaca cttttattat catcttatat gaaatcacct tttccagttt taattacaaa
     3361 tataatatta ctatttgtac caatattttt aaaatatagt gaaacaaata agttgtataa
     3421 tcaaattctc aatgttttac catcaaatgc tatttcattt tcatttacta attatgtaga
     3481 ttataaattt ggtaatttaa taatagcttt acctacaatg attattttta catgcttagt
     3541 tcttacagtt gtttttttac cttttattaa aaataatttt aaaaaacatc aagttaacta
     3601 aaatatatat tataaaaaga aggtgtctta aaatgaactt tttagcttat taggcactct
     3661 tctttgtatt aatttaaata tattttagta ttagattaaa tatgttttca tcattttaac
     3721 aatgaaaaag agctttatct ctattttgag atagctctta taaagttaaa tattaaattg
     3781 ataactataa aatatgtagt ttgaatttat aatttgtgtt ataatcatac tgatttttgt
     3841 acatcagttc ttacattaac attgcctgct tttagatttt ctccaatagt aagacctttt
     3901 tcttttagaa catattcaaa agtcattagt ggaactccac cttttctacc acctaatatt
     3961 tcggttcctt ttaaatcatt gtaagagaaa ttaggcattt ttttcctagc aactagaaaa
     4021 ctaccatctc tttttgtcaa ttgagcaaag ttcactgcat aatcgttttt gttttgatta
     4081 tatacatata tagatgcttc aggacccatt aaacctacgt ttgcttcacc tgaaatcaaa
     4141 gctgccatag ttttatctgc accttgggta tttataaggt caatctttat accttcatct
     4201 tcaaagaatc ctttcgtaat agcagcatac tgtggagcat aaaaaacaga gtgagtaact
     4261 tctgctatag ttactttatc cagattttta gacgaagaag attcaccttt tttgtcatta
     4321 gagttaaaat cacatccagt tagacatatt gtacttacaa tgatagagct tgctattgcg
     4381 actatttttt taaacataat aaatacctcc cataagacat acgaatagta tatagtatta
     4441 aaaatttatt gtagttgtta caaaaatgta tctatgctta taatatttct acaaaaactt
     4501 aataataatt ttcagcttta tgtttagttt tttc
//
LOCUS       DAMJYX010000180         4511 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJYX010000180 DAMJYX010000000
VERSION     DAMJYX010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.185"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            231..1310
                     /locus_tag="PC479_003426"
     CDS             231..1310
                     /locus_tag="PC479_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2805939.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC479_003427"
     CDS             1634..2002
                     /locus_tag="PC479_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2805940.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC479_003428"
     CDS             2119..2922
                     /locus_tag="PC479_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2805941.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="PC479_003429"
     CDS             3314..4204
                     /locus_tag="PC479_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2805942.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1714 a          489 c          848 g         1460 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctagagt tagtattata gatgaatatt
     4441 agtattgata ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata
     4501 ttctaggtaa t
//
LOCUS       DAMJYX010000181         4463 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJYX010000181 DAMJYX010000000
VERSION     DAMJYX010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.186"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            49..717
                     /locus_tag="PC479_003430"
     CDS             49..717
                     /locus_tag="PC479_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805943.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            876..1382
                     /locus_tag="PC479_003431"
     CDS             876..1382
                     /locus_tag="PC479_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2805944.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1510..2526
                     /locus_tag="PC479_003432"
     CDS             1510..2526
                     /locus_tag="PC479_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2805945.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2880..3584
                     /locus_tag="PC479_003433"
     CDS             2880..3584
                     /locus_tag="PC479_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2805946.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            3879..4358
                     /locus_tag="PC479_003434"
     CDS             3879..4358
                     /locus_tag="PC479_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2805947.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT         1871 a          375 c          764 g         1453 t
ORIGIN      
        1 tatattatat taggtatttt aaataaattt tttaaagggt gattttttat ggaaaatata
       61 actagttatt ttacaacaat actatgtgta tttatatgct tatcatctgt atttatattt
      121 actcaactag ctagagtatt tattaataag aagaagatta atcaaaaaat taaatcaaga
      181 aacggattta ggtatgatag agattttatt gaagccagaa gagaagaaat acatataaag
      241 gataacaata ataataaaaa taagagtaac aataagaaat taaagggaga aaaagtattt
      301 aaatacgata atggtgattt atataaaggt gagtttgttg atggaaagaa aaatggattt
      361 gggatatata ttttttcaag taaggaaaag tatgaaggat tatggaaaga tgacaagatg
      421 catggaatag gaaaatatac ttatagagat ggttcaatat atacaggtga atttaaatac
      481 ggattaaaaa atggtttggg aaaattaaca tatcccaata atgacattta taagggatat
      541 tttttagata ataagaaaaa tggtaagggt gtattatata aaaatgatgg aaataaacaa
      601 gcaggaatat gggaagatga tgaacaatgt aaaagtttag attttaaaga tttaaataat
      661 aataaaataa ataatgttta tcaaaataga ttaaataaca ataaattaaa agtttaatta
      721 ttctaataaa aaaatcttta tttatgatgg aatataggat atatctatgt tgtaatttgt
      781 aaaatcttcc tatatcatat aaaattaagt attgattagt ctattacata aatatagaat
      841 ataaatatat ccatttaata ataaggagaa gagatatgaa agagtatgta gttgtggatt
      901 tagaaactac aggattagat ccatataaag gttgtgaaat aatagaaata ggaataacag
      961 aaataaaaaa tgaacaaatt gtaaaaaact attcaaggtt gataaaacct aaggggataa
     1021 tttcttcatt tataacagaa ttgacacata taagtaatga aatggtagaa aatgaagaac
     1081 cactagaatt agttttacct agatttagaa aatacatagg tgatagaact ataattgcac
     1141 acaatgcgaa atttgattta aaatttttaa attactatct tagaatgtta gatttagaac
     1201 caataaacaa ttatatatgt acagtagagt tattaaaaaa atgtaaaagt tataaaggaa
     1261 aaaacaagaa acttgaaaca gcttgtgctt attacaacat agagaatata aatgctcata
     1321 gagctgacag tgatacatta gcaactgcaa aactattttt gaaaataaaa gatgaatatt
     1381 aattataaat atagagaaaa ttagtgaaaa tatcctttat aaaattgacc cttaagctta
     1441 caattttgtt ctttgattta ttttgtagaa caatgtataa tgtaccttat atgaagaagg
     1501 gagactaaaa tgggttatta caatgataat gataataata aaataagacg taaaaaaatt
     1561 agtaacagca aagatagtaa taaaaaatct aaacattctt atactccggc aaataaaaaa
     1621 aataaaagtt ctaagacaag ttctagtaaa agaatgagtg aaaaagtaga ttcacagaat
     1681 cttataaaaa gagatagaga tagttttaca agagataatg taagaccttt aaatacagat
     1741 tacaatcaaa gagaaagaag aagagtcgag tataaacaaa gagagaaaaa attaaaaagt
     1801 aaaaaaagaa aaaaacaaaa aaggattaga atagttagag cattattatc tattatgtta
     1861 gttgcactaa ttatattttc agtggcaggt gcaatattta cagtttcagc tataaaaggg
     1921 tcaccagaag ttactaaaaa gttaattagg tctaaatata taagtagtga ggttgtaagt
     1981 ataaaacaaa tgccaaatga tttaaaacat gcaatagttt ccatagaaga tgagagattt
     2041 tataagcata aaggagtaga tgttatttct ctagttaggt cagtattaaa caatgtgttt
     2101 acagatacaa cacaaggtgc aagtactata gatatgcaag tttcaaaaaa cctgcttact
     2161 agtaatgata aaactatgaa gcggaaaata agggatatgt ataatgctaa gcagatgaat
     2221 aagattatga ctaaggatga aattcttgaa gcatatttaa ataatatgta tctaggtaaa
     2281 aatgcatatg gagctgcaaa gggagctgaa gtctactttg gtaaaaaagt tagtgaatta
     2341 aatttagctg agtgtgctat gttagctggt atgacaaata atcctgcaag gtatattgac
     2401 catggagagg ctaagaagag acaagtgatt gtactgtata agatgcatca gcttggttat
     2461 ataactgata aggagtatag aatagcaaaa gctgatgaca ctccatttaa atcagaaatt
     2521 gattaaaatt gtaagaatat agtattttca atagtttaaa caaacaaaaa tgtaataaaa
     2581 aaggtgattt gccacccatt tgccaccata attataatct aatggcaaat tgttttaatg
     2641 tacaaactag ataatagaat attgtcttaa atgctatatt atgctaaaat ttaattgtat
     2701 tttgtaaatt aaaaaactta taatttagtt ttctagggtt ccgcatttta taatggtcag
     2761 gtccaagaga aaacgcgcaa ttgttgtgta cacggaagga taaaagcctg ggagatattt
     2821 aagaatattt ctataggctt ttttattttt acatatttta aatttgagga ggttttctaa
     2881 tgaaaaaatt tgttattgag gatgcatttt ggaatctatt tcctagtgca aaaattggag
     2941 ttgttatttg taatgatata gataactcta taaaagatga agactataag aaaataattt
     3001 tgaagggaga agaagaagca ttaaaatact tggaggactc agaatttagc aacaaccaag
     3061 tgattaaagt atggagagaa gcatttaaaa aatttaaaac gaaaaaagga gcaaggtctt
     3121 ctattgaagc attattaaaa agagttcata atggaaataa tataggaact attaaccctt
     3181 tagtggatat ctacaatttt atctcattaa aatatgcatt accttgtgga ggggaggata
     3241 tagataaatt tataggagat attaggctaa ctagagcaat tggtaatgag gagtttatcc
     3301 cactagggac agatgaaaat tcatttccat atgaaggaga aataatttat aaagatgatg
     3361 gtggagcaat ttgtagatgt tggaattggc gtgaagcagt gagaacaatg cttactgaag
     3421 atactaacaa tgccttttta tgcattgaat tagtagatga aagtagattt aaagaatttg
     3481 aaaatgcttt aaatgaatta gctaaaatag ttcaagaaaa attgggtggg aattgtaaaa
     3541 tttcaatttt agatattgat aataaagagg tatcagtgga ttaatatgga ggtgctgttg
     3601 taatggacta ttctagttat aaagatactt atatttaggg taagaaggtc tattttagat
     3661 ataacgatta tcaaaggata taatatagaa aataaaaatg tagaatataa aaagccttta
     3721 aaataagaaa aaaatgtatt ttaaaggctt ttattatact cttttttatt tgcataatta
     3781 acaataaaaa tatattgtta attataatat ttttattgaa aaacgatata atataatgta
     3841 ttatactata tataaaatat tttaaaggag ttggcatgat gtctaataaa cttataaaaa
     3901 atttactaag tggtatatta caaattttat tttttctttt aggattagta attgttgcag
     3961 gaggatttaa aagttttatg tacctctgtt ttagtggaga agcaactcta caaggtacta
     4021 tttatggaat attaatgttt attttaggtg tatcatattt tatcattata aaatcattga
     4081 tagaagtatt aggtagttct gaatatagcc tatttgtaaa agagaatgta aaaagattta
     4141 ggattattgg atatcttttg cttttaaact ctctaattga gtttatttca acttttggta
     4201 ctactggaaa aggaatgaga tttctagatt taggatttgg attttatttt actgttccag
     4261 tatttgtata ttttataact tctcttatga gttttgtaat tgcagatgga tttgtaaagg
     4321 caataaagat aaaagaggat aatgatttaa ctatttagat gtgacacttt aatatatagg
     4381 agataaataa cccgaaaaga gtgactaact agtaatttaa aataaaatat attagggtat
     4441 aatacataaa ctataatgct aaa
//
LOCUS       DAMJYX010000182         4364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJYX010000182 DAMJYX010000000
VERSION     DAMJYX010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.187"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            142..1617
                     /locus_tag="PC479_003435"
     CDS             142..1617
                     /locus_tag="PC479_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2805948.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1704..2117
                     /gene="speD"
                     /locus_tag="PC479_003436"
     CDS             1704..2117
                     /gene="speD"
                     /locus_tag="PC479_003436"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2805949.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2147..2998
                     /gene="speE"
                     /locus_tag="PC479_003437"
     CDS             2147..2998
                     /gene="speE"
                     /locus_tag="PC479_003437"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2805950.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2988..3866
                     /gene="speB"
                     /locus_tag="PC479_003438"
     CDS             2988..3866
                     /gene="speB"
                     /locus_tag="PC479_003438"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2805951.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4046..4246)
                     /locus_tag="PC479_003439"
     CDS             complement(4046..4246)
                     /locus_tag="PC479_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2805952.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1722 a          494 c          748 g         1400 t
ORIGIN      
        1 ttcaaaaaaa cacttgtaaa aatgctgaga tgttaatata ataaatatca tgagtttaat
       61 aaaagtaaaa ataaaaaata gaaataataa ataataaata ttaaaaatat ttgaatatag
      121 agaggagatg aatttgaaat catggatagc gagtttgcaa atgcagttga attaagctat
      181 gagtgtgctc cattattaga gtcgttaaag gaatactcag agaaagatat agcttgtttt
      241 gatgtgcctg gacatgtaaa aaatagaggt gtagctatat taaataaata tctaggtgaa
      301 agtctcatga aaatggatat aaattcatca ccaactatgg ataatgtatc tgcgccaaat
      361 gggattataa aaaatgcaca agatttgcta gcacaagcat atatggctga tgaggcattc
      421 tttataacga atggcacaac tcaagcaata catgcaatga ttttgagtgt tataaaacca
      481 ggtgaaaaag tattacttcc aagaaatata cataaatcag taataaatgc gttaatactt
      541 tgtggaggaa taccaatatt tatacaacca gaatttgatg aaaaactagg tataagttta
      601 aatattactc ttgaaaaagt tagaacagag atagaaaaag attgtaatat aaaagcatta
      661 tttttcctta atccaactta ctatggagtt tgtgcagacc ttgagagtat tatagaacta
      721 tgccataaaa ataatgtttt agtgttagtt gatgaagctc atggtgcaca ttttccattt
      781 catttagatt tacctccttc tgcaataagt ttgggagctg atatggtagc tgtaagtata
      841 cataaaactg gaggagcatt gacacaatct tcagcactct tattgaatag agataatgta
      901 agttttgaaa aggttcttca atcaatcaat atgctacaat caacatcagc atcttatttg
      961 cttatggcaa gtatagatgg tgctagagtt aatctagttg agaatggtga aaagcaactg
     1021 tcaaaagcat taaatctatc taggtatgca aaatctaaat taaacaaaat tgatggaata
     1081 aaagttttat ctacagaaat attgaaacaa aaaggtgtta aatttataga tgaaacaaaa
     1141 ttgtgtataa atgtaaaaga attaaatttg actggatttg aagtatacga tttattatat
     1201 aaaaattttt caattcaagt agaattggga gattcatata atatactggc acttgtgtcc
     1261 attggaacaa ataaatcaga tatagataga ttagtaaaag cactttctat aattgcaaag
     1321 atttatagaa aagaatcaac attaaatgaa tttaatatgg ttcaaataaa tccaattatt
     1381 aaacttaatc caagagaagc tttttatgca ccaaaggaaa gtgtagaaat caattcatgt
     1441 atagatagaa tttgtggaga atcaataatg gcatatccac caggtatacc gattattgct
     1501 ccaggagaat tgataacaga agaaattatg gagtatatta ttttcttaaa aaatagcaat
     1561 gcatatctta cagatgtaca agataaaaac ttagatagaa tattagtaat aaaataaatt
     1621 actttatttt gcaagggcaa gtatttgtta agtagagttt atcaaataga acttattaaa
     1681 agtataaagg agttggggtt gaaatgaaat ttgaaacact aggaagacat atactagtag
     1741 aatactataa ttgtgatgaa aacgtactta aagacccacg tcttatagaa aattttatga
     1801 atgattcagc attaaatgca aaagcgacaa ttgtggattc tgtattccac cattttaatc
     1861 cttggggggt ttcaggagct gttataattg ctgaatctca tttgacaata catacatggc
     1921 ctgaatatgg ctatgcagca gcagattttt ttacttgtgg tgatatagac ccatggaaaa
     1981 gttttgaatt attagagcaa cttttaaagt ctgaaagaag cgagtctaca gaaattccaa
     2041 gaggcttaac tacaaaaatt aaaaaatatt caaaaaaaga tttaggtaag attactcata
     2101 aaccagaagc agtataaaac atacaagaga aagtgaggat tattatatgg aactttggta
     2161 tacagaagaa tggacagaaa atgtaagatt ttctataaaa gtaaataaac atctatttga
     2221 aggaaagtct caatttcaaa ggatagatgt gtttgatagt gatgaatttg gaaaatttct
     2281 aacaatagat ggattaatga tggtaacata taaagatgaa tttatatacc atgagatgat
     2341 aactcatgtt cctatggcta caaatttaaa cataaagaaa gtattagtca taggtggagg
     2401 tgatggaggt acagtaagag aattaagccg ttatcctcaa atagaaaaga ttgacatggt
     2461 tgaaatagat aaaatggttg tagatgtatc taaggagtat atggatatat gctcatgtaa
     2521 attagatgat aaaagagtga gtttatattt tgaagatggt gttaattttg ttaagtgtgc
     2581 acatgataaa tcatatgacc tgataatagt agattcaact gacccaatag gaccaggtga
     2641 agggcttttc tcaactgatt tttataagga ttgttataga atactgactg atgatggaat
     2701 tttagtaaat caaagtgaaa gtccgtattt tgactttaat gctaaagaga tgaaaagagc
     2761 taataaaaaa ttaaaacaga tatttccaat atcagaagtt tatcaagcac atataccaac
     2821 atatccatct ggtcactggt tgtttggatt tgcttcaaaa aaacttaatc cagtaaaaaa
     2881 tcaagataga aatggatggg agaaattaag tttaaaaact aaatattata atagtgatat
     2941 acatttaggt tcgtttatgt tacctcaata cgtgaaggag atgctagatg aagaataatt
     3001 tctatcatat gaacactttt atgagtatgg acaaaaatta tgaagaatct aatcttatag
     3061 tatttggtgt tggatttgat ggaactactt ctaacagacc tggggctaga tttgcaagta
     3121 gctctatgag aaaagaattt tacggtcttg agacatatag tcctttttta gatttggatt
     3181 tagaggatta taatatatgt gattatggag atttagaaat tagtgttgga agtacagaac
     3241 aagtcttaaa agaaatctat caagagacat ataagattgt tagagattca aaggtaccct
     3301 ttatgattgg aggagagcat ttagttacat taccagcctt taaagcagta catgaaaagt
     3361 acaatgatat atatgtaatt cattttgatg cccatactga tttgagggaa gaatataata
     3421 atagtaaaaa ttctcatgca acagtaatta aaagaatatg ggatattgta ggtgataata
     3481 aaatatttca atttggtata aggtctggga caaaagaaga atttaaattt gctacagaag
     3541 aaaaacacac atacatggaa ataggaggaa tagatacgtt tgaaaatata gttaatatgc
     3601 taaatggaaa gaatatttat ctaactatag atttagatgt attggatgca tctgtgtttc
     3661 caggaacagg tacaccagaa cctggtggtg taaattatag agagtttcaa gagattttta
     3721 agattataaa aaactctaat ataaatatag ttggttgtga cattgtagag ttaagccctg
     3781 attacgatac aacaggtgta tcgacagtta tagcttgtaa aatcttaaga gagttatgct
     3841 taataatatc tgataaaatt aaatagagta ttttatataa atagtatata ttataatata
     3901 agaagttatt ttaaaaaagt tagtttaagt ttgacgcttt tctaaagtaa cttcttattt
     3961 ttgtaattaa aaataaatat aataaacaac aaaaaagagc cctcgatagc tccttttcta
     4021 aaatatatat atttaaatta aataattata ttctatttac gttttcagct tgaggtcctc
     4081 tattaccttg agttatatca aagctaacct tttcgccttc ttctaaagtt ttgtatccat
     4141 cattttgtat agctgagaaa tgtacgaaaa catcatcttc tccctctaca gatatgaacc
     4201 caaatccttt ttcattgtta aaccatttta ctattccgtt tttcattata agaaaacctc
     4261 cgcattatta aaaaatacaa actaaaaaaa taagtctgtt ctattaatat aacataaatt
     4321 ttaaataaaa gcaaataaaa tttttaaaaa aattaattac tttt
//
LOCUS       DAMJYX010000183         4323 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJYX010000183 DAMJYX010000000
VERSION     DAMJYX010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.188"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..1170
                     /locus_tag="PC479_003440"
     CDS             <1..1170
                     /locus_tag="PC479_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2805953.1"
                     /translation="ENQKYLYPNIYNKNDFNVIIDGKNYGLPNDNMQLNEKKPFLKLK
                     TRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSEKGIHRKTDIPNEFSGY
                     ILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHNLKPNLEIGYINNLIKL
                     EKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGLYTWFYKGRDSIVKQTF
                     SNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMADKIKVISESLFEKINSI
                     KTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNAQNRKKIMLEIEKLFKK
                     YNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYYNLIYTKSEENENE"
     gene            1157..2152
                     /locus_tag="PC479_003441"
     CDS             1157..2152
                     /locus_tag="PC479_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HDF2805954.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPK
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            2154..2894
                     /gene="cas5b"
                     /locus_tag="PC479_003442"
     CDS             2154..2894
                     /gene="cas5b"
                     /locus_tag="PC479_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2805955.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            2918..>4323
                     /locus_tag="PC479_003443"
     CDS             2918..>4323
                     /locus_tag="PC479_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HDF2805956.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEI"
BASE COUNT         1893 a          381 c          666 g         1383 t
ORIGIN      
        1 gaaaaccaaa aatatttata tccaaatata tataataaaa atgatttcaa tgtaattatt
       61 gatggtaaaa actatgggtt accaaacgat aatatgcaat taaatgaaaa aaagccattt
      121 ttgaaattaa aaacaagaat tagaaattca actccttatt tgattagtga tgatgaagtt
      181 ttgttacaga aagctttttt tgattatcta acgaataaag cagcgaatgg agaagaagta
      241 atatatataa gtagcgaaaa gggaattcat agaaaaactg atataccaaa tgaatttagt
      301 ggttatatac tgagaataaa aaagggtaaa gaattagaaa tacatgattt tgatactgtg
      361 gtaaatttaa acactaaaat aaaaagaata aatgtagaat gtataatacc aatagaatat
      421 gaaagacata atttaaaacc aaatttagag ataggatata ttaataatct tataaaattg
      481 gaaaaaacga ttgatactgt attctttaat aaatttatgt gcaatagtta ttttaaagaa
      541 attaaaaata taaacttaag tgatttagtt ctaagggata tacttattag acatagagaa
      601 ggtttatata cctggtttta taaagggaga gatagtatag taaagcaaac tttttcaaac
      661 tattctatgg gtataattga aaattctata aaaaacaata actacataag agcaaaggag
      721 caatataatt taagaatagc tataataaat tattttcatg gaggaagtaa tatggcagat
      781 aaaataaaag ttattagtga atctttattt gaaaaaataa attctataaa aacgaaatgt
      841 atacaaagtg atgaagaata ttattttgca gttgggcaat tagcatcgta ttttatatct
      901 aaaaataagt caaaaaataa aaatcattct ctgattaatc ctatactaaa tgcacaaaat
      961 agaaaaaaaa tcatgttaga gatagaaaaa ttatttaaaa aatataatta tgatataagt
     1021 tatgacaaca ataggcgatt taagaatcta tattctatga ttgtaggata tgaggtagaa
     1081 gaaaaaaaag ttaatagaga tttattaatt ggtggatatc tatattacaa tttaatatac
     1141 acaaaaagtg aggagaatga aaatgaatag aagagtatat ggagtattag gtataaaatc
     1201 tataatgtca aactggaatg ctgatttttc tggatatcca aagacgacat caacaggaga
     1261 agtttttggg agtgataaag cacttaagtt tcccatgaaa aaaatgtggg aaaatgaagg
     1321 tgaaaaagtt ttatatgtta aatcattaaa aatagatgaa tctaagaaag gagaaataaa
     1381 aattagacca aaatcattaa aagagagata tgaagaattg tttgatatag aggacctaaa
     1441 actagaaaag aatcaacaaa aagtactgga aaatctattt aaagctattg atgttaaaaa
     1501 ttttggtgca acatttgcag aagaaggaaa taatatagga attatgggcg cagtacaaat
     1561 tggacaagga tttaataagt atgaagatat taatgttgaa gaacaacaaa tattatcccc
     1621 atttagggat ccaaaagcaa aagataagaa aaagtcaaca aatgaagaag aaaaagaaga
     1681 tgcaaattca actacattag gaaaaaaaat agtaagtgat gaggctcatt acttttatcc
     1741 attttcaata aatccaaaag cttatgaagt ttttgttaat atgggtgtga ctgatggata
     1801 tactaaaaat gattataaaa aatttaaaga agctgcttta gtaagtgcaa ctgcattcaa
     1861 tacaaattca aagcttggtt gcgaaaatga atttgcatta tttgttgaaa ctgatgaaga
     1921 gttataccta ccagatttgg cacaatatat aacttttgaa aagagagaaa aatcaaaaga
     1981 tttaatttgt ataaatataa ttcatcttat agcagaatta aaagatgata taaagagcgt
     2041 tgagatatat tataacccat ataaaataga aataaagcct tttaaagatg aaatattacc
     2101 taagatagat aaagttaaat ttatgaacat atttacaaga aaagaggtat aatatggagg
     2161 ttttgaaatt taacttaagt ggaaaaactg ctttttttaa aaaaccagat gtaaatagca
     2221 taatttattt tacgtatgga aacatacata aaattgcact tatgggtatt ttaggagctt
     2281 gtttaggact tggtggatat aatcaacaga gtaaggaaga tgagtatcca gaattctact
     2341 ctttacttaa ggatataaaa atgtcaatag ttccaaagca tgaatttgga tatataaata
     2401 aaaaagtcca tatatttaat aattctgtag gatatgcatc aaaagaagaa ggtggaaatt
     2461 taataataaa agaacagtgg cttgaaaatg ttagttggga tatatatata caattagaag
     2521 attcaactac aagtagagag cttaaaagaa gatttttatc taataaattt gtatatatac
     2581 cttacttagg caaaaacgat catatagcta atataagtga aatagagata attactgatt
     2641 tttacaaaac agaagatatt aacagagtgg acagtttctt tataagagag gattttgagt
     2701 ttgaaaaatc ttattttgat gataatgatg attataaaga gccatttaaa tatcaggaaa
     2761 aacttcctta tagactgcac cgaatgacta atcaatatat tttggaaact atcatattta
     2821 ctaatatgtc agttaaattg attaataaaa aagaggtata tcaagtaaag ggtttaaatt
     2881 taatctttat ttaaatttat aggtttagga gaattatatg tactttaaaa aagttgagtt
     2941 gtttgatata agtaataata taaataattg caaaaatatt tatgctcata taaataaaga
     3001 caggaagcca gaaactttaa aagaacattc agacttatca tactcatatc ttatgaaatt
     3061 atgtgtaaaa aaagatttaa gctcttgcct agagagaata gagaataaat tttttaaaaa
     3121 agattctttt ttaagtaaga atttttatag agagattatc ttgaatatgg tttatatgca
     3181 tgatataggt aagattaatt cttcatttca gtttgtaaaa atgcaaaata aaaattttaa
     3241 aaaaatttca cttagatata ctaaacattc ttttatctct tcaatgctat attttgatta
     3301 ctattttgaa aagcttaaaa aatatgattt tgaagaaaaa aaaatattga gtttattttt
     3361 gatgataaat acatatacaa tatcaaaaca tcatggggtt ctgaagtcat ttaaagaatt
     3421 tttattttta tttaaagatg agtttataaa cattaaagaa aattttaaag atttatttaa
     3481 aaacaattat aatttagagc ttaaatcagt tcccaaaact atagaaaaaa tggtaagagg
     3541 tgtagaagcg tatttagatg atagccatat aaaggataac ttagctatag acttatatat
     3601 atattcaaaa ttgatgtttt caattgttac atcttgtgat ttttatgcta catcagaata
     3661 taatagtggt aattctatag atgattttgg gacaattgat aattataata agtattatga
     3721 tgtattaaaa aaatctgaaa tatataaggg gatgaaaaaa tatagggaat acttaaataa
     3781 caatagtgaa tgcccatata aagagaatga tataaacaga ttgagaacag aaatgagtat
     3841 agaagctgaa gatgcaatta cacataatac gagaaaaaga atattttatt tagaagcacc
     3901 aactggaagt ggaaaaactg ttacatctat aaatctagca ctgaaatcta tagagttaaa
     3961 tcaatcttta aaaaagatat tttatatatt tccatttaac acattagtag agcaaacaaa
     4021 agatgtattt ataaatgaaa tatttaattc tgttaaagac atacaaaaag atgttgtagt
     4081 tataaattca ataacaccaa tacaaacaga agataaagaa gaagataata aaaatgttga
     4141 atattgtgtt actaaacaag aaattgatta tgataaatca ttgttaaata gacaattttt
     4201 acactatcct atagtactta caactaatat aggttttttt aactatttgt ttggtgtatc
     4261 aagagaagaa tgttttccat tgatacatat gataaatagt gtaattatat tagatgaaat
     4321 aca
//
LOCUS       DAMJYX010000184         4257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJYX010000184 DAMJYX010000000
VERSION     DAMJYX010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.189"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            184..936
                     /locus_tag="PC479_003444"
     CDS             184..936
                     /locus_tag="PC479_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2805957.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            923..1804
                     /locus_tag="PC479_003445"
     CDS             923..1804
                     /locus_tag="PC479_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2805958.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(2030..3385)
                     /locus_tag="PC479_003446"
     CDS             complement(2030..3385)
                     /locus_tag="PC479_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2805959.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         1674 a          614 c          520 g         1449 t
ORIGIN      
        1 cctattttta atagatttaa tattatgata aatagattta taagattaaa tatttataag
       61 actaaatatt tctaatatat tttatttttt tcttaatata gttgacattt tttcccttaa
      121 attataaaat caaattaata actacatagg tagttactaa tcttttatca aaggagtttc
      181 tatatggata atattattaa tgaattacaa aatataggat ttactaaata cgaatctcaa
      241 atatacatat cattattaaa ggaaagccct ttaacaggat atgaaataag taaattatca
      301 ggtgtaccac aatcaaaagt ctatgaaaat ataacaaagc tattaaacaa taatataatt
      361 atcaatgttg gaactgaccc tattaagtac ataccaataa accctaatga attattaaaa
      421 aataagaaaa aagaatttga caattctatg gataatctaa agaatagctt acagatatta
      481 aataaaagta aaagtgtaga atacgtatta aacataaaag gagataaaaa tatattaaaa
      541 aaagcaatag aaatgataaa aaatgcaaaa caagaaatta taatttcttg cccttatgaa
      601 gaaattcttg aattaaaaaa agaactccta attgcttaca aaaaaaatat taatattgaa
      661 atactacttt tagaaaacaa aagtctgaaa ggtcttgata atattcatat tcatggaggt
      721 aataaaacta cacttgaaaa taaaatgctt tacaaaggaa ttattttgat tgtagataat
      781 gatgaaatat tgacaggaaa cttatcagaa ggcagtgagg ccatttcaat ttggacaaaa
      841 aactctaaca ttttatatat tggagttcaa tacataaaac atgaaattta tatttcagaa
      901 caaataaaag gaggttataa aaatgaacaa aattaagatt aaacaagttg atgcttttac
      961 aactatacct tttggtggaa atcctgctgg tgtcgttact gatacctcta acatttctga
     1021 tgaaattaag cagaatgtgg ctaaagaaat gaatttatca gaaactgcat ttgtttcaga
     1081 gtcaaatatt gcagatttta aagtacaatt tttcacacca ggtttagaag ttgatttatg
     1141 tggtcatgca acaataggta ctttccatgc acttttggaa gaagggaagc ttaatactaa
     1201 taaaagtata ttttatcaag aaacaaaagc tggtgtatta tctgtagagc ttaagaaaat
     1261 aaataatgaa aatatattta tgatggaaca gaatacacct gaatttgaaa atttagataa
     1321 ttgcagaaaa gaacttgcac aaatgatagg tgtgcatgaa gatagacttc ttgaatatcc
     1381 tattataaag gtaaatactg gactttggtg gcttgtattt ggtctaaaat ctttagatga
     1441 tttaaaaaat atctctccag atttaaataa aatcaaaaaa tttagtggtg ataataatat
     1501 aattggtata actccatttt gtatagaaac tatagataaa aagtgtgact accatttaag
     1561 atctatagca ccatatgttg gagttacaga agaccctgta tgtggaactg ggaacgcttg
     1621 cgtatcatct tatatagtac atcacaatat tttagataaa actagtttta ttggtgagca
     1681 aggtaacttt ataaacagac ctggaaaggt atatgtaaat ataactagag aaaacaataa
     1741 cataacttct gtaaaaattg gaggaagtgc ttatactgtc ttaagtggtg aaattatgta
     1801 ttaatcccat acctaataaa aacaaataaa aatactagct aactaaagct agtattttta
     1861 tttgttattt ttatttaata atcatactct aacaaatatt ataattatat atatcataat
     1921 attgtattta gaagtaaaat tttattttta ttattaatta gttataaatc aatctaatac
     1981 aacataatta cagtagatta acctataaaa cataataatt gcttactgat tatttatcgt
     2041 cctcaacaag tctacttaat gtcttatcag ataagaataa taatatcgct gcaacaaata
     2101 gtattactgg aattatagta tatactttaa tcatatcaaa tttaagagta aatgcatata
     2161 tatatccata tcctttacca gctataaaag tagcaaatgt ccatactccc ataagaacac
     2221 ctagaagctt cttaggtgca tacttactta caaaagagtt accaagtggt gagaataaca
     2281 tttcaccaag acttaaaaga ataccaaaca tgattatcca tagtatacta gctttagcag
     2341 tttcaggagc tccaactcct cttgaaaatt ctgcaccaac aagcattaag aatgcaagac
     2401 ctaagaatat taatccaaga cctgtctttt taaataaact catatctcct tgtggtctac
     2461 tagcaagttt aaaccataat gctccaagaa ctggtcctaa tactatacat acaagtgaat
     2521 ttaatgagtc aaaccaagca agaggaacat caaatcctcc aacattcatg tttacaaatg
     2581 ctgcaccata atcataaact gcaagataag ttagatacca gaatacccag aaaattacag
     2641 agaagaatga tataagtata atagcccaaa ttctcttctt atccatgctt gtaagaggag
     2701 acttatcttt ttcttcaatt ttttcagcaa ctacaccttc tttaaaaggt ctctttcctg
     2761 cattcccaag gaatctccat ccatatatga accatattgc acctattaca caaagtacag
     2821 ctgctataaa gaaacattga gaaaatccaa gtacaccatt ttttgcaaat gttttaaggt
     2881 aaagaatacc aactgctata gtaccaataa aagaacctat attaacaaaa gaatattgta
     2941 ctgaaaatgc tgtatcaagt tcttcttgac tatcaaaaag ctgtccattt accgcagata
     3001 cgttaccttt aaagaatgca gtaccaattg aaactaagat tatcattgca tttaccatag
     3061 aaactgtagt tgcaatagaa ccaagatagt atcctacccc catgataagc atacctatag
     3121 gtatagtata tctagctcct atccatctat ctgatatata ccccccaatt ataggtgcaa
     3181 ggtatgtgaa tgcaacaagg ttggcttgca ttatagcagc ttgtccttta tctattccaa
     3241 gaccaccatg aactatagct gttgtaagga atatatatat taagtacttt gaagcataat
     3301 atgctgctct ttcaaatgag aaagttgttg agcagatata aaatcccata ggaaatttac
     3361 ccttcttttt tactgttgag ctcataattt taaatccttt cttaattgta tttttatttt
     3421 ttacgttttg tccaaattct ttcttttatg aaagatataa tattattatt ttgtaaaaca
     3481 atttgtaatt attattaaga taattgcgca tttatttgta gagtacatat tctcatggaa
     3541 taaaattaat acacttctta atcataaatt taaattatat aaattcacta ttttcagata
     3601 tagttataaa ttaatatatt ctcatctaat accatgaaaa aaccttcgcc atatgcaatt
     3661 cttatttttt acgtgatagt attttacaaa ctaaaatccc ccttttgtag caacttatgt
     3721 aattacattt tagtgctttt ttaaaggagt ctcgaataaa gcaacatata ttttgttcaa
     3781 ataatgtttt tatttatttt ttaaatttat atattacttc acccaaccaa ttctcaactt
     3841 aaataactaa actactatct attcacagat taggcttatt tcttacacat tagtttataa
     3901 taaacactgt gaatctaaac ataaaaactg ttattttaaa gtaattaccc atatattcgc
     3961 tttgatacta tttattgagg ctatactctt tatgtataat atattattta taaaatacct
     4021 caaaatatat tattcataaa atacctcaaa ctgaaaactt tttcacgaat atttattttt
     4081 cacctcccct tttttatttc accatgtaaa tcgtaccata aatatgcact tatatgcaag
     4141 aaaaaaatct attaatcgac agtaaataat tagaaaaatt caaattatat ttatcaatta
     4201 tattatataa aatatttcct atgaaaatat aaattaaatt aatgttcaga atagttt
//
LOCUS       DAMJYX010000185         4251 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJYX010000185 DAMJYX010000000
VERSION     DAMJYX010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.190"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(280..1197)
                     /locus_tag="PC479_003447"
     CDS             complement(280..1197)
                     /locus_tag="PC479_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2805960.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1459..1836
                     /locus_tag="PC479_003448"
     CDS             1459..1836
                     /locus_tag="PC479_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2805961.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1992..2441)
                     /locus_tag="PC479_003449"
     CDS             complement(1992..2441)
                     /locus_tag="PC479_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2805962.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2605..3009)
                     /locus_tag="PC479_003450"
     CDS             complement(2605..3009)
                     /locus_tag="PC479_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2805963.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(3122..3988)
                     /locus_tag="PC479_003451"
     CDS             complement(3122..3988)
                     /locus_tag="PC479_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2805964.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1398 a          695 c          463 g         1695 t
ORIGIN      
        1 ttttttttat taacatttca tttttcagtt gattgtttaa tatatttcat tttttatagt
       61 aattttatat ataactacga aatataaagt aattagataa gtaacaagaa actaacatgt
      121 aatgttagct actctagata tgaaatctta aaacttttaa atattgaaat atttctttat
      181 atatattatt gtatatttct tataaattat tgtcaattta ttttttctta aaaataaaat
      241 aattgcataa tttatttcaa attatgcaat ttataaaatt tattcccaaa actcttttac
      301 tgcttcgatt atctcactat aaggcaataa agtcgcttcg attattgatt tatttgtctt
      361 ttctaaataa tacttaatca tataatatcc acaagcatat cctgcacaat aaggcaaccc
      421 tactggaaaa tatccttgca ttttagctat atcatcacca taaagataag atgttatatt
      481 ttgaaatcca gtttctttta aagcactttt tattattggc tttatataag tatttaatgt
      541 ttcgatatct gttctactga cccaaggtcc taacatttcc tctccaaaca tccatgttgc
      601 aaaattttct gcaagacctt catttatcat catttcttct aatgttatat tattattcca
      661 ctctataaac tgaaatctaa tattgtgatt acattcatgt gctattaata ctggtaatct
      721 attgatagta tattcattag gaaccaatga tagaaatata tatccaggaa tcccaccatc
      781 accccagtat ccatcactta atattgtata aggactattt ggattcgcca ataatattga
      841 atatttatag tccttaatgt ttaaatcata cccttctttt ataaaagcat ttatagagtt
      901 ttctatggtt tctttgcaag tgccccaaat tttatcagca gataacacat ttatattctt
      961 tttttgcttt tcatcaatat ccttaggcga taaaacccct aacatttcac ttgctataat
     1021 tacatcatat ccacctttct gtctagcaac tattggaaca ttcatacact tccacttaaa
     1081 ttcaaaaggc ttcattaatt catatctgta tatatcttct tttttgttta ttggtgcatc
     1141 cattatcttc ttgtatattt tatctgagcg aatttctata atattcatat ttaccatctc
     1201 ctttaagaca aagtatatac aattacgtag cgtcaatgtc aatatacatt ttttataatg
     1261 tcaccttaat ctttttatta tattttatcc tgtaaacctt ccgtacatat aacatttttt
     1321 taaattaata aatttgtata atataataat aaaaaattat atacttcaag gtttccctat
     1381 gatacaattt actaaatcat aatttagatt atcaggtgca actataaatt attctatatc
     1441 aaggaggata tattggtcat gctaactcca tatttaatat ttaatggtac ttgtgaaaaa
     1501 gcatttaatt tttatgctga ggctttcgga ggaggaaaaa ctatatttgc gcgattagac
     1561 agcaatccaa acaatcctat tatgcacgca agtgttactt tcacaaaata cgaaggttgt
     1621 ataatgggtg cggatacaga caagcctgtt gtaatttctg gcatggcgat ttgtgttgtt
     1681 ctaccatctc gagaagcgat agaagaaata tctgtaaaac ttgccgaagg tggtacactt
     1741 gtacaagaat ttttaccaca cccaccacca catcaaaatg atggcgctgc tgaagtactt
     1801 gataggtatg ggtatacttg gtatttaagt acatagttaa aataaaaaaa ctacatctaa
     1861 taatcataat atagaacaga taaatttcaa tttaatagtg tagtaaaaaa gtcgtgtcct
     1921 aagactgtca cgacttttat ttgtataaaa atttaagtaa atatttttgc gaataagtgg
     1981 gaagtataac tttaaattaa tttcatatgt agtattggaa atgggttccc ttgtccatct
     2041 atttcagacc tatcaaacac tttaaatcct aagtgaatat aaaatcctac agctttttta
     2101 ttctgctcat ttacatcaac atattttaca ttgtatttat ctatagcata ttttattaat
     2161 tttttaccaa taccatttcc tctatattta tcttctataa agagcatctc aattttatct
     2221 ttatctattc ctataaagcc ctttattata tcattatctt ttatacaaac tatattttct
     2281 acttctttaa gtccaatctc aactaatggt atcaaagatt caatatcttt ttctgttaaa
     2341 aaatcatgcg ttgctcttac tgatgattcc cagacttgca acatttcgtc atattctgtt
     2401 gaatttattt gtttcatttc aagaattatt ccagatttca taaaaatttc ctccctatat
     2461 cacttttaat tgcataacac ttttttatac aaaacttaga aaggactacg aaggttaaat
     2521 atcatagtcc tcattaagtt taaatattat ctattatata ttatactata atctagcaaa
     2581 ttcactacaa taattcacat cagcttatac taaaggtgct tctatttgag tagtaaaaag
     2641 gaatttatga caatgaccat cattaacagt agtttctcca catattaagt gaatgtgttt
     2701 atcagttcca ggtattctaa ttgctggccc tgttgtaaca catatcttat ggaaatggtc
     2761 aaggaaatct acattatcat ttattttatg aacatgattt gtacctccat ttattggtat
     2821 aacttcacca gtaacccctg ctgcacggtg gttatgtcta tcctcacatt cttctgctaa
     2881 cttaacactt tctgaatatt catgaacatg tgtcatttcc tcataatcat tatagcactt
     2941 gttgcactta ttgtatttat aatcatcata cacataatct ctttcacatt tataattttg
     3001 ataactcatt tttatatctc tccctatatt atattaagaa attcttgtaa agaatctcta
     3061 atgtataata tgaattaaag tcaaaaaagt tcatattttg taaaattatg tattaattta
     3121 attatttttc ccagtattta gggcgtatta gtgaagctgg taacttcgta ctagaatctc
     3181 cttttgcagt atttacttgt aattgtgtta aaaatatact attttcaaga ttagcacctc
     3241 taatatcaca atctcttaag tctgctccta ttaaatttgc cccactcaaa tctgtatttc
     3301 ttaaatttgc tgctattaga aatctacctc ttaaatctgc tcctattaga tttatttttc
     3361 taaggtctgc tcctattaaa tctatatttt tacttaattt tttcatgttt ttaagagtag
     3421 aagtcttgat aaatgattta acctttccca tcatagactc actcgcttgt gataataata
     3481 aattaacctt tgttctatgt gatatgatat ccaaattaat tatctgttct gggcccatat
     3541 tagttatctt ttcagtttct tcaattatta aatcaatttt tgatttgatt gtatctgata
     3601 tatttaatac agatgcttct ttaagatacc ataacatttc atgtaattgt ctcataatta
     3661 aaaacacttc agacatttga tttgttagtt ctggtgattg catccagtct atccccttat
     3721 atgttacttg tgctactttt tgacctgaac caaaacaatc atatgcagta caccctttaa
     3781 aacctcgctt aataagacta ttatgtacag aacatttaaa atctggttgt aaattaatac
     3841 agggttttcc tgattcctta ttgataggaa atccatctga agcagaaaaa taaagagcta
     3901 cacagcaaag tccaaagcac ttcttacagt caatctttag ctcctcaaat aacttattat
     3961 catatatatt tttatgtata tctatcatat tttttctcct cttaaacttt atttttaata
     4021 ttatattatc tataactaaa tgataatcaa gtcccttatt taacagttct tattcttaat
     4081 tataatttaa ggtattattt attccaatag catacataag tttctttata cttgtaacaa
     4141 tacctttata ctaagactta ttttaaatgt agcactgtaa taataaattg tttatatact
     4201 cataatgtat ttgataaatt gtaaatcttt atagaagttg aaatttttaa t
//
LOCUS       DAMJYX010000186         4227 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJYX010000186 DAMJYX010000000
VERSION     DAMJYX010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.191"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            189..1400
                     /locus_tag="PC479_003452"
     CDS             189..1400
                     /locus_tag="PC479_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2805965.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1593..1853
                     /locus_tag="PC479_003453"
     CDS             1593..1853
                     /locus_tag="PC479_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2805966.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            2207..3919
                     /gene="ptsP"
                     /locus_tag="PC479_003454"
     CDS             2207..3919
                     /gene="ptsP"
                     /locus_tag="PC479_003454"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2805967.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         1731 a          399 c          765 g         1332 t
ORIGIN      
        1 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
       61 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
      121 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
      181 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
      241 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
      301 ttggtgcttt aaatggagca tatatagttc aaaatgatat tgaagtaatg gaagatattt
      361 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
      421 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
      481 gaaaaaatga aggtatagat ataagcccat taagacaact aatggagaca acattaaatg
      541 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
      601 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
      661 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
      721 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
      781 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
      841 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
      901 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
      961 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
     1021 gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt aatttattga
     1081 atataaaaag agatgcttca cttagaaccg ttatggaaag tgtaatacca aagcttggtg
     1141 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
     1201 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
     1261 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
     1321 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
     1381 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
     1441 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
     1501 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
     1561 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
     1621 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
     1681 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
     1741 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
     1801 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
     1861 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
     1921 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
     1981 atttaatcag aagattaaga ctattgttaa ttgggtataa aaatataaat attttagaaa
     2041 atgaaataag atatttttaa tcataaattc agagtaaaaa tacagtaaca aatagtttat
     2101 aaaattttat aaaaaattat aagtaaataa aactataaaa atgtaaatta tttatataaa
     2161 tttataaagt ttatgtgcta gatgatattt tttaggaggt agggttatgg cttacaaagg
     2221 aataggtgct tctcctggtg ttgctttagg gaaagcgtta gtagttgaac atagtgaatt
     2281 agtaattgaa aaaaaatcta tagataatgt tgaagctgaa attgctaaat tagaagatgc
     2341 agttgcagtt tctaaagaag aattagtaaa agttaaggaa aaggcttctg aagaattagg
     2401 agcagaggaa gcagaaatat ttgaagcaca tttattagtt ttagaagacc cagagttaat
     2461 tggttctgca atagataaaa ttaaaactga aagtgtaaat gcagagtatg ctttaaatga
     2521 aataaaagag atgtttgtta gcatgtttga gtctatggac aatgagtaca tgaaagaaag
     2581 agcggcagat ataaaagatg ttacaaatag aatacttaga catatattag gaattaaagt
     2641 tgttgattta tctgctttaa gcgaagaagt tgtattaata gcacatgatt taactccatc
     2701 agacacagca actatgaata aaaaaatggt tttaggattt ttaactgata taggtggaag
     2761 aacttctcat actgctatca tgtctagaac tttagaaata gctgcaatcg ttggattaaa
     2821 tgatataact tcaaaagtta aggatggaga ttttgtggtt ttcaatggag acacaggtga
     2881 agtaatcgtg aatcctgatg aagagactat aaataaatat actgaattaa aagctaagta
     2941 tgaagatgag agaaaagcat tacagctatt aaaaggaaaa ccatctgtaa ctttagacgg
     3001 aaaacatgtg gaattagctg gtaacatagg aacacctaac gatatagaag gtctaataaa
     3061 gaatgatgct gaaggtgttg gtctttatag aacagaattt ttatacatgg atagagattc
     3121 tttcccaaca gaagaaatac aatatgaagc atataaagct gtattagaag gaatggatgg
     3181 aaagcctata gttataagaa cattggatat aggtggagat aaagaattaa gttatttaag
     3241 tatggaacct gaaatgaatc cattcttagg ttacagagca ataagacttt gcctagatag
     3301 aaaagatata tttaagactc aattgagagc tttatataga gctagtgttc atggaagatt
     3361 aagaatcatg ttcccaatga tttcatcttt agaagagttg ttacaagcta aagaagttgt
     3421 aaaagaagtt ttagcagaac ttgattctga aggtgtggct tatgctaaag atgtagaaat
     3481 tggtatgatg atagaagttc catctgctgc tgtgatatct gatgtattgg ctaaacacgt
     3541 agatttcttc tctataggaa ctaatgattt aattcaatac acttgtgcag ttgatagaat
     3601 gaatcaaaag ataagctatc tttacaatca atttaatcca gctgttttaa gacttataaa
     3661 gactgttata gacaatgcac ataaagaagg caaatgggca ggtatgtgtg gagaatctgc
     3721 tggagaccaa aaaatgatac caatactttt aggtatggga ttagacgagt tctctatgtc
     3781 tccaatatca atacttcctg ctagaaagtt aataacttca gttaaagagt cagatatgaa
     3841 aaaattagct gatgatgtac ttaatatggg tactgctgaa gaaataaaaa gctatataga
     3901 aaagactttt aatatataat taatgttata tagttactaa aattcatttt tctaaataac
     3961 aaaattatat tttacgaatt aaaaaggaga tgtttcaaaa taaaaatttg aagcatctct
     4021 tttttataaa agattactaa gaattactta atctttgtaa tagtcatttt gtgaaaaagc
     4081 aaaactgcat tgtggctata gtatatatgg atgttattat caataaaagt actattaaaa
     4141 tgaagattca ttctcaaatg atatcaaaag tagttgtgat ggagtaagat aatattttgt
     4201 gatttaatag taaaaattaa aaaaata
//
LOCUS       DAMJYX010000187         4214 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJYX010000187 DAMJYX010000000
VERSION     DAMJYX010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.192"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(238..1110)
                     /locus_tag="PC479_003455"
     CDS             complement(238..1110)
                     /locus_tag="PC479_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2805968.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1504..2082)
                     /locus_tag="PC479_003456"
     CDS             complement(1504..2082)
                     /locus_tag="PC479_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2805969.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2140..2856)
                     /locus_tag="PC479_003457"
     CDS             complement(2140..2856)
                     /locus_tag="PC479_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2805970.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3047..4132
                     /locus_tag="PC479_003458"
     CDS             3047..4132
                     /locus_tag="PC479_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2805971.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
BASE COUNT         1303 a          652 c          445 g         1814 t
ORIGIN      
        1 taattaagga aatgatattg tttagagaaa taaaaaaagc actctccatg aatggaaatg
       61 ctttctttaa taaattagtt gacgtagtaa agatgtattc agaatacatc tttatgtttt
      121 gagtctcgaa atatttattt gtattattta aacgaaaatt tattttttaa gtgataatcg
      181 agtatttact aataaggcta tgattctcaa tttcatagcc ttattaatat acaaacttta
      241 ctcatacaca gaatatcttt tccaaatttg ctctaaatca tcaaaatgct gtttaatatc
      301 ttctttttcc ttcatcccaa cagatatagc aagggcattt gaaatactaa gagctggaac
      361 tagagaatct acaaatgaag ccatattact cttaactaga agagtgttat ctgcaagtga
      421 tgctactggc gcaaataagc tatctgttaa tgatattaca tgggctcctt tttctttagc
      481 ataattgaca atttgataag atttcttaga ataacgaggg aaactaatag caactattac
      541 atcttcttca tttattctta caatctgctc aaaagcatct cccatatcca ttctaattat
      601 atgaacattg tcaagtatta tatctaagta aaaacctaaa tattgagcta ctacaaaaga
      661 actcctcatt ccaagtatat atatttttct cgctcttaaa agtttgttag aagcttcttt
      721 aaacgctctc tcgtcaattt cttctagtgt agaccttata ttatctatat cactttttaa
      781 aactttgttt agtattgcaa aatcatcgga ataatcatgt gccatttcaa ctctttgtac
      841 tgtagttagt ttatttttta tgagttcttg aagtgcagct tgtaatttag gatatccact
      901 ataacctaat gcatttgcaa atcttacaac tgtagactca cttactccta cggtttcacc
      961 caatttacat gcagtcataa atgcaacttt atcataatta tttaaaatgt attgtgctat
     1021 aagtttttga cccttactca atctcgtata ttgagattgt atatttgata tcaaatgttt
     1081 actgtccttc atttcatttg tttcttccat ttttttctcc ttttagttat ataaactatt
     1141 ttgttttatt aacactgtct ttttctctaa aaattttatt ttttatctca atttcatctt
     1201 agtttttatc atagcttctt aaactcataa aattctaact tttctgtacc ttatacttca
     1261 catagtattt tcgattaaat ttattatacc atctttcctt gcatttttta atatttatcc
     1321 aatatttctt ttaaattttt caatttttac tcatttaaaa gttatctatc ttattttgca
     1381 tatttagatg tatattttgt tttgagaata aactgttgta tacttattat ttaaagtatt
     1441 tttaataggt cacactatat atattaactc ttaatacaat cttgtcaagt tttttagtat
     1501 ttattatgct tttttctttt caattaaaat caattgtggt gggttattga cttgatttaa
     1561 aaacttagat tctaaaacat taaattcatc ttgatttaaa ttattgacaa ggttatatat
     1621 gcaatctttt tcttcttgac ctccatcatg ccctgtatat atggcaatgc ttattactcc
     1681 attttctttt aacatttcta aacttaactt tatagccttt aaagttgtat ctggtttggt
     1741 tataattgaa tgtttcgctc ttggaagata acccaaatta aataaaacac atgaaacttc
     1801 ttcttttata tattgattca ttttttcatg cccatcaaat atcaatttta ctctatctgt
     1861 atatccttct ttctctagtt tctttcttgt agactttatt gcttcttctt gtatatcaaa
     1921 agcatataca aagccctttt ctccaacctt ctcagctaga taccttgtat catacccatt
     1981 tcccatagta gcatcaatta ctatgtcacc ttcatttata atattttcta aataaaattt
     2041 atttatatct gtaattttag ttaaatactt tgcatatgcc ataatactat ttctccctac
     2101 tattaacttt atttcatttt ttctcaattt atttttatct tattttattg attttaaata
     2161 ttttatttca tcttctgtta ggtatctata ttctccaact ttggtgtctt ttaatactat
     2221 tttccccaca gaaatccttc ttaaatttaa aactggatga tttatagctt tgcacatttt
     2281 tcttacttgt ctatttctac cctcatgtat agttatttca cacactgaat aatttttttc
     2341 tttattttct ttagttaccc ttattttggc aggtgccgtt gtgtaatctt ctatttttag
     2401 tcctgtttcg aattgtctta tctcatcatc acttataatt cccttcacac ttgccacata
     2461 cattttatca atttcatgct ttggatgggt aagcttatat gttaaatcgc catcattagt
     2521 aagtattaat aaaccactag tttcataatc taatctccct attggatata ctctttccct
     2581 tacatcttta agtatgtcta ttacactggg tctattgaat tggtctttta ctgtagttat
     2641 atatccctct ggtttattta atactatata tatataattt tcacttggtt ttactttttt
     2701 attatcaaat tctactatgt ctttttcttc atcaatatta aaagataact cttttactac
     2761 acttccattt acctttattc ttcctccaag aattatttct tctgcttttc ttcttgatgc
     2821 tattccacaa gaagctatat acttattaat tctcatactt aatccttcct tatcttaaat
     2881 tatgtgtgtt taatattttt atcaatgtta attttgctac attattatac tatactagac
     2941 cttaattaaa aacaagcaaa aaaattattt aattttccta aataattgta atttcttttc
     3001 acatactatt aatatgtaat agctgactaa acactatttt aaggaagtga ttattatttc
     3061 aaaggaaaat ataaaatatt atttaatagt cgtgtttatt ggaattttat tttttaaatt
     3121 tattaatact ccatcagact ttatatctag tattgaagga ttcttaaagt tttttagccc
     3181 atttttatta gctattttac ttgctttact tttaaatcca cttgtgatgc tttttgagat
     3241 aaaattcaaa gcacatagac ttttagccat ttttttatct tatatattta taggttttat
     3301 tttagccttt gccattaggt tattaattcc atcaatagct aatacattaa atagattgat
     3361 aaatgaaatg cctatgtata ctgattatat agatagtttt atagaaaaaa atatgtcaaa
     3421 catatatttt ctaaaaactt taattcctca tatacaacat agtttagata atttattaaa
     3481 agaagcaagt aattttgttg gtaaaattcc taaaaacttc ttaatctata cacttagtat
     3541 atcttctatg ctatttaata tgacaatggg atttatacta tctatataca ttatatatga
     3601 caaagaaaaa attgcattgg gatttaagag atttctatat tcttctactg ctagaaacaa
     3661 ggcagataac attatagaat tttttaggac tactcatgat attttctatg attatttact
     3721 tggtagaata ttagactctc ttattattgg tataattgca tttttaggat ttcaatttgt
     3781 tatcagaata gaaaatgctt tatttttagc ttcaataata ttcttaggta atataattcc
     3841 ctattttggt ccttttattg gtgcaatccc tccaatagcc atgactatat tatatagtcc
     3901 acaaaaaact atatgggtca ttgcatttct atttatattg caacaattag atggaaattt
     3961 tatagaacca aaagttatgg gtaatcaagt tggtatagga gctatatggg taatatctgc
     4021 tattttggtg ggacaatctc tatttggttt cataggagtt tttctttcag taccaatagc
     4081 tgctgttgta aaaacttatg ttgataaata tatagataat cgtttacaat agattatctc
     4141 tactgtgact ttaattttta gtttaaaatt taactgctat ttattaaatt atttctcaaa
     4201 aaatatctct tagt
//
LOCUS       DAMJYX010000188         4168 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJYX010000188 DAMJYX010000000
VERSION     DAMJYX010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.193"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(343..723)
                     /locus_tag="PC479_003459"
     CDS             complement(343..723)
                     /locus_tag="PC479_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2805972.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(924..2117)
                     /locus_tag="PC479_003460"
     CDS             complement(924..2117)
                     /locus_tag="PC479_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2805973.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2180..3436)
                     /locus_tag="PC479_003461"
     CDS             complement(2180..3436)
                     /locus_tag="PC479_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2805974.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
BASE COUNT         1511 a          750 c          425 g         1482 t
ORIGIN      
        1 tttattaata ttttttatta tttttttaac aaactatcag agtagaatta tttaatattt
       61 tgttattttt aatcaaatga acgaatatta tatatatcat tcataaaaac tttatataaa
      121 gaatagaaac aagttctaga ttactataga gatgacatat caaaatattt ttaaaaagta
      181 atttaagtat tagaagtaat tgaagttagt aaatataaat gcgtttactt ttaagtattg
      241 cttaagggaa taaaaaataa aaataaactc tgcaaccatc taaaaatagt tgcagagctt
      301 acaaaaccaa aattatctaa attgtaataa aaataataac atttatacat aagcttcttc
      361 ccaatttact acccagtttt ctaacgactt tatcttttcc tctgttagct cctcttcgtg
      421 aaatgatact aataaattcc ttataaaatt attcttcaaa agattttgca catttcttga
      481 tataaactct aaatatgcct tttcttttat tgcaactgta taatacatac acttgcctgt
      541 ttcttgaaca tataagaatc ttttctttga tagttttgat aaagttttta aagctacttt
      601 ttctggtaaa ttatgttctt ctttcatata atcaataatc tcatatgaat tggtttttat
      661 atctaaattc caaataaatt tcataataac taactctaat tttgataatt tttttaaaca
      721 catagttatc actccgcttc tttatctata taattaaata ttacaataat aatttcacaa
      781 aatcaattct aataatcata acatacctta aaatctgttc ttaacttcta ccttcttaat
      841 ctcccaaatg atttttttat attgtattca atagtataat ccttttccaa aatataactt
      901 accagttaaa ataatcttta tttttactta ctctcgcctt ttacagaagt taataatttt
      961 ttataagtta catcaattcc aaaagctcca agaggtgcag ttataagtat agctagaact
     1021 gctaacgtca aaataagttg tccagatgcc atccccattg caagtggtac acctcctatt
     1081 gctgcctgta ctgtagcctt tggaatatag ccaatcatac agaacaacct ttcctttatt
     1141 gtcaaatttg tcttaataag gcagaagaat actcctacca tacggaataa aagaactcca
     1201 aatattaaaa ttacagctcc aattcctgct ttaaatgcat aacttatatc tactgttgcc
     1261 cctaccaaaa caaacaagag aatttcagcg ccaacccata gttttgaata ctttatggac
     1321 aatcttcttg caactacacg atattttatc tgaaggaaaa tcccaatact cataactgct
     1381 aataatccag aaaatggcac aataccctct aaagcagtct ctaaacttat cattaaaaat
     1441 gagatactta aaagcaacac ccctttagca gaatctctca tatgaacctt cttaaaaaat
     1501 actgctaaac agattccaat cactgctcca actattacac ctaatataat tgatactgga
     1561 atttgtaaaa aactaatagc tgatatacta tttccttgtg ccaatcctgt gaatactgta
     1621 aacatcacaa ttacgaaaat atcgtctata gaagttcctg ctaaaagcat ctgtggaata
     1681 cttttttctg taccataacc ttcttccatt aatttaagca ttttaggtac aataattgcg
     1741 ggagaaactg ctcctactac tgcacccatg actgctgctt ccaatataga aacgcctagt
     1801 aattttggtg ctaaaactat cattccgata atttcaaaag tagctggaat aaagcacata
     1861 agtacagcag gtcttcccac tttcttcaaa tcgtttatat ctaaagataa tccagccctt
     1921 gttaatataa taatcaatgc aatctttcta aggtcagaag aaatatctag tattgaatta
     1981 tcaatcaaat ttaaaacata tggacctaat ataattcctg taagtaacat tcccaataag
     2041 ttaggtaact ttatcctctt aaaaatacct cccgaggcca ttcctaataa aaaaatcaaa
     2101 gctaaacttg tcaacatatt cctaccctat cctttctcta atattctttc tcttctaatc
     2161 atacattatg ttgttttatt tattgttttg ttgaaatttt ttgtagtgaa taaatagatt
     2221 tatccatatt aaatatatat gaaatagcgc atactacaaa gaaataaggt aaatagctgt
     2281 atccaaaaac ttctgcccct ataaatacag gtgcaaaaaa agtatttgta gcactaccaa
     2341 agacactagc atagccaagc gctgcaacta attcaattgg caaattaaaa aatcctgcta
     2401 acaatacacc aagactagct ccaatggaaa acaaaggtgt aacctctcct ccttgaaaac
     2461 ctgctgacaa agtcagtatt gtaagtataa actttaacag ccaatcaaat gaataaattt
     2521 ctccaccata gaaactattt tgaattaaat ttgtccctaa acctgaatat cttcctttat
     2581 aaaataaaag gaataataca ctaagacata ctccaataat agcaattctt atcataggat
     2641 tttttaatct attacctatt tttctttttg acaatttcag acaccatgca aatgctccac
     2701 caaccattcc aaaaataatt cctaaaacaa taagcttcca aaatattgac aagtcaaata
     2761 caactttaga agaaagtgca aaagaaaatt tctctaaccc taaagctttt gatactgaac
     2821 ttgctgtaaa agaagcagta aacgcaggaa aaagcgattg atactccagt gaaccagcta
     2881 ccaaaacctc catagcaaat agtattgctg ctattggtgt ctcaaataat cctgcaaaac
     2941 cagctgccat accagttact agaaaaatac tacaagcatt ttttataggc aaccttttac
     3001 ctacccaatg tgaaaaagtt gcaccaatct gaacagcaac tccttctcgt cctgcactac
     3061 caccaaataa atgggttaac catgtacctg atattataaa tggaaccaat cttaaaggaa
     3121 taatttcttc ttctccatgc cctacctcaa atattagatt cattccttta ctactttttc
     3181 ctccaaattt aaaatagcaa taagcaataa caactcctac tactgctaaa aatggtatca
     3241 gatacattgg atatgtttct cgaatatctg taacttttag aagaaccgtt ccaaatatgg
     3301 tgtctataag acctataatt gcaccaactg gtatacctat gagtcctaaa aaaaatagtc
     3361 caccatatgt tttataaacg cttttaattc ttattatcat tttaatccat attaaagttt
     3421 taaatttttc tatcatttta attcctttct atttgcatca taagattttt tgaaagtttt
     3481 attacatcaa ctgtagtcaa tgtaatgact atcataattg ttttttaaat tgataggata
     3541 taggtacact cttaacccat tcttaataaa ataattaaca tataatctac tattttttct
     3601 attaattaaa tatttcttca aatttaaaaa ataaaaattt agtaagtact tcacctccat
     3661 tttattgatt taaacaacaa aaaaagctgg tggcatctat gatatcatag aagtcatcag
     3721 ctcattggca gtttaggata ttatcatcca agggagaacc tcattcccat aattattata
     3781 taatttttta tttttcatat gtctatttta tgtaaattgt atttttaatg ttataaataa
     3841 tataacctat caattaaaat agtaaagcct ctaatattat gagtgttcaa tatttcatat
     3901 tatttttatg atatataatt taaatgattt tctttacttt gcattatgat acataactta
     3961 aatatttttc ctcactccac attatgatat attatttaaa tatttttctt cactctgctt
     4021 atattatgat acatgattta aatattttct tcactctgct tatattatga tacatgattt
     4081 aaatattttc ttcactctgc attatgatac atgatttaaa tatttttact cactctacat
     4141 tatgatatat tatttaaata tttttctt
//
LOCUS       DAMJYX010000189         4155 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJYX010000189 DAMJYX010000000
VERSION     DAMJYX010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.194"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            474..1382
                     /gene="trxB"
                     /locus_tag="PC479_003462"
     CDS             474..1382
                     /gene="trxB"
                     /locus_tag="PC479_003462"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2805975.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1472..3295)
                     /gene="typA"
                     /locus_tag="PC479_003463"
     CDS             complement(1472..3295)
                     /gene="typA"
                     /locus_tag="PC479_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2805976.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3507..3749
                     /locus_tag="PC479_003464"
     CDS             3507..3749
                     /locus_tag="PC479_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2805977.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3897..4088
                     /locus_tag="PC479_003465"
     CDS             3897..4088
                     /locus_tag="PC479_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2805978.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1518 a          543 c          669 g         1425 t
ORIGIN      
        1 ctaatattta aactattaat cataattggt tcaacaaaag tatatagtta ataatgttta
       61 atttgaagaa attgttagtg tgaaattaca tatatattca aattaaattc ataaatattt
      121 cacataataa tagtatactg taaacaaaag gaggtattaa atatgggaat agtacttata
      181 acagcagcat tagttttggt tatgtatgga ctttcaattc tatcatatat atcagaaagc
      241 aatatattag aagacactgt aaatgataac caataaggtt ttgaattata taaataagtg
      301 agaactgtaa ataaaagtta atttttattt gcagtttttt tatatatatg taaaataaag
      361 ttaattaata aaaaataatg ttttgtttat agtatatatg gataataata gtaaagtata
      421 agaatgatga gaataatata taagaaataa taatataaag agaggtaaac gttatggtag
      481 atatcattgt aattggtgct ggaccagcag gtttaacttc agcaatttat gctatgagag
      541 cagggttaag cgttacagta tttgaaaaaa atatttatgg aggtcaagtt gcaagtacct
      601 ctgaggtaga aaattatcca gcagttcaaa agatttctgg ggtagaattt tctaataata
      661 tatacaatca agcagttgct caaggtgttg atattcagtt tgatgaggtt gaagagataa
      721 atttagaggg aaaagtaaag gttgtaaaaa cttcttcagg tgaacataaa gcaaaggctg
      781 ttattcttgc aaatggagtt gaaagacgta aattaggttg tgcaggggaa caagaattta
      841 caggtagagg agtatcttac tgtgcaacat gtgatggagc cttttttaaa gataaagaag
      901 ttgctatagt aggtggagga aatactgcac tagaagatgc tttattttta gctaataatt
      961 gtacaaaagt gtatttaatt cacagaagag atagttttag aggtgaagaa gtgttggaga
     1021 aatctgtgaa agcaagagag aatattgaga tactttacag tcatggtgta gaaaaaatag
     1081 aaggagaaaa gactgtatcg aaaatagaag taaaaaactt aaagactgaa gaaaaaagaa
     1141 ctatagatgt tagtggtatt tttattgcta taggtcttaa acctaataat aaaatgtttg
     1201 aaaatgttct tgatttggat gaaggtggat atataatttc agatgagtct tgtataacat
     1261 cagtagaagg tgtatatgta gcaggagata gtagaactaa gtttttacgt caaataataa
     1321 cagcagcatc agatggagct attgcagccg ttcaagcagc taattatata aatgttgaat
     1381 aatataaatt tcaaataaaa atagccatat aaactgaaat aatttttcag tactatatgg
     1441 ctattttgtt aattagaggt aaatgtacgt tttatttacc ttgtctagat ttgttatatc
     1501 ttaatctatc atgttcattt aagaatcttt ttctaagtct tatagaatca ggtgtaattt
     1561 caactaattc atcatcttct ataaactcta gtgcttcttc aagagtaaat attctaggag
     1621 gagataattt tatagcatca tctgaaccag aagcacgtac gttactcatt ttcttgtttt
     1681 tacaagggtt gactaccata tcatcttttc tggaattcat accaattatc ataccttcgt
     1741 aaacttctac accagggtct acaaacatta cagctctatc acttaaggca ttaagagaat
     1801 accccatagt aacaccaggt ccttgtgcta ttaaaacacc atttcctctt gaaggtattt
     1861 ctcctttaaa ttcttcaaat ttttcaaatg aacgaactaa agtaccttct ccacgagtag
     1921 cgtttataaa ttcacttcga tatcctaaaa gaccacgagt aggtgctaag aactctattt
     1981 ttacatagtc tccttctatt gacatagact ccatcatacc ttttctcatg tttaattcat
     2041 ttataatagt accagagtaa acttcaggac agtttacaac aactctttct attggttcca
     2101 ttaattttcc atcttcttta tgcattaaaa cttcaggttt agaaacacca acttcataac
     2161 cttcacgtct catattttca agtagtatag ataagtgaag ctctccacgt ccagaaactt
     2221 tgtatccatc agtagtatca agaggttcaa ctttaagacc aacatttact tcaagttcct
     2281 tttcaagtct atctttcaaa tgtctagttg taacgaattt tccacttttt ccaacgaatg
     2341 gagagtcgtt tactaagaag ttcatagaaa gagtaggctc ttctattttt atcatttcca
     2401 taggtagtgg actatctaaa tcgcatatag tttcacctat agatatatca ggaataccag
     2461 ctataacaac tatttctcca ctagtagctt catcaacttc aacttgtttt aaaccttcat
     2521 acacagaaag ttttgatact tttcctttag atacaacaga atcttctcta catatagcaa
     2581 cttgtgtatt gtttttaaga gtacctttgt aaattctacc tataccaagt ctacctacat
     2641 aatcatcata tgcaagagct gatatttgga actgtagagg ttcattatcg taattaggat
     2701 aagcttcaac gtgatttaca atagttttaa atagaggaga aaggtcttca ctatcatcat
     2761 ccatttcaag tttagcaata ccttgtttag caataccata tattatagga aattcgcatt
     2821 gctcatctgt tgcatttaag tctacaaata aatcgaaaac ttcattaact acttcttccg
     2881 ctctctggtc ttttttatca atcttgttta taaataatat tggttttaaa ccgaactcta
     2941 aagatttttg taaaacgaat ctagtttgag gcattgggcc ttcactagca tcaactaata
     3001 gaataactgt gtcaacagtt ttcataacac gttcaacttc agaagaaaag tcactgtgtc
     3061 ctggagtatc aactatgttt attttgtaat cttcatagtt tattgcacag ttttttgaat
     3121 atatagttat gcctctttct ttttcaaggt cgttactgtc cataacgcag tctttaacta
     3181 cttcgttctt tctaaaaaca ccgctttgag aaaggaaagc atcaaccaat gttgattttc
     3241 cagcgtcaac gtgggctata actgcgatat ttataatttt atgtttttgt gacatttttg
     3301 ttgctccctt cgattaaaat aacttattaa ttatagcaca atatattatt aggtacaagg
     3361 aggaatatat taaattgtat taatatagtt taaattgaat aaaactgtta gttttgcatc
     3421 tgaaaattat tttgtttaaa aaaatttttt agacaaataa taaccttaat agataaaaag
     3481 attaataaaa gagtaaagga tgatatatgt tggaagtaac caaaataata attaaatatt
     3541 ttgcaagtga aagtgatact tttgtaaaag aagaagtttg taattttgaa gaacttctta
     3601 agtggtctca taaatttatt gaaggtaaaa agatagaaga tgttttgtat ataaagtcta
     3661 aagaaaacta taaagtaaat gtgattaagt ctttagaaat taaggaattg gaaatagtga
     3721 gaaaacaaca gataccaccg tacatgtagt attaataata tataaaatta atatgtaata
     3781 ctcgcaatag gatataaatg gtatatatgg taataattta tattaaaaaa acttttataa
     3841 agtttatatt tgggtataaa agtattaatt agacattaat taggaggaac ttaaatatga
     3901 taagagtatg tccaaactgt tcaaatgtgg atgtaaataa gctaaaaact ttggttggag
     3961 aagaaaatgt aaagactggt tgtataggtc agtgtagagc atttaaaaaa gaagctgttg
     4021 gaataataga caatgagctt gaaataaagg aaacagaagc agagtttttt gaagcatgta
     4081 aagaataaat tttaaataaa atataataaa tagagcatct aaattgtata agcattaaac
     4141 aatttagatg ctcta
//
LOCUS       DAMJYX010000190         4078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJYX010000190 DAMJYX010000000
VERSION     DAMJYX010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.195"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(175..1035)
                     /locus_tag="PC479_003466"
     CDS             complement(175..1035)
                     /locus_tag="PC479_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2805979.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1184..2074
                     /locus_tag="PC479_003467"
     CDS             1184..2074
                     /locus_tag="PC479_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2805980.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            2292..4046
                     /locus_tag="PC479_003468"
     CDS             2292..4046
                     /locus_tag="PC479_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2805981.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1668 a          431 c          638 g         1341 t
ORIGIN      
        1 agaacggatt tggaaatact tgtataaaac aataattgat acatttaatt tttatcttaa
       61 aaacaacaat tataatatat aaatagcaag tataatatat aaatagcaag tataatataa
      121 catataaaaa gttgaatttg tatatgttaa ttatacttgc tatttatttg ttagttattc
      181 tattttttta aatccattac ctaaagcttc atgtatatct gtaattataa taaaagcatt
      241 agggtcgaca ttttttacta attttttcaa ttcgacaact tctttctttt caactactac
      301 taaaagtaca tttttatttt ctccagtata tgcaccttct ccttttagaa tagttgcacc
      361 tctatctaaa tcttccatta tttttttact tatatctttt gaaaggtcag atataatagt
      421 gaatgatttg gaataattaa atccttcaat aagcaagtca ctaactttta ctaaaatgta
      481 taaagccaca gttgaataaa gtgcaatttc aacctgtcta tttactatac ctgataaaac
      541 aactacaacg cagtctacaa tacccattaa aacaggtata ctaaatgtag gaaaatattt
      601 atttagtagg agtgccatta aatcagttcc acctgtagag ccatttattc taaaaataag
      661 tccttgacca actcccatta aaatagaacc agatataatt gcaagataca tatcatttgt
      721 tatatctaat gtagccatat ttgcagtaag cttaagtgct atggtaagaa aaattattcc
      781 taaaaccgtt ttaaaacaat ctttttttga aagtattttg taagcaaata tgaataatgg
      841 aacatttaaa agtaaattga caatccaaag ttctacaccg aataatgaat ttaaaacaac
      901 tccaagtcct gttataccac cagcagcgat tgcatgtgga ttaaaaaata agttaagaga
      961 aatggccatt aatatacagc ctacaattaa tattaaaatt tctgaaaaag ttattctatc
     1021 ttttttcaat aacatcactc cttatatata gattataata tatattatat agtttttcca
     1081 atttttaata aaagaaataa aaatatatta taaaaacaat ttgattggaa ttttcagaaa
     1141 attaatgcta taattaaaag taaatttaaa gggaggttat aaaatggcta aattaactct
     1201 agaacatata aaaggaaata catattatat tccactccct acaatagtag gtgtatacgt
     1261 agatggtaag gatgcaattt taattgacag tggaaataat aaggatacag ctagacaagt
     1321 tttaagatta ttagaagaac ataatttaaa tcctaaatta ataataaaca ctcattcaaa
     1381 tgcagaccat ataggaggaa atgcatattt gaagaatcaa acaaaatgca agattgctac
     1441 gactaaaatt gaaggatatt ttacagaaaa tccaatatta gaatcagcat ttttatatgg
     1501 tggctatcca agtaaagcac tgaagaataa atttttatta gcaaaggaaa gtgaagttga
     1561 ttatattatc ccttcaaatg gcaaaattgt agatacagaa cttgaagcga tttctcttcc
     1621 aggtcattat tttgaaatga ttggtgttag aactccagat aatgttttgt ttgttgcaga
     1681 tagtcttaca ccagagaata taattactaa atatcatttt ttcttcctac tggatataga
     1741 ttcacagttt aagacactag aaaaattgag aatgttagag gctgatttct ttgttccaag
     1801 tcactctgtc aaaactacag atataaaaag tttaattaat attaataaga agaagatgga
     1861 agaaataata gataatatta aaaaggtttg ttatgagcca gttatgattg atgaagttat
     1921 tgaaaaaatg tgtgatttgt acaatgtcaa gcttgatgct aatcaatatg tgttggttgg
     1981 aagtacaata cgttcttata taacttattt atatgagaat aatatggttg aatatatatt
     2041 tgatggtggt aagatgatga taaaagctgt gtaaatagta ataattttca aattatatga
     2101 tgaaattgtt ttagtatgac tgttatttga aaagtaatat aaaaaaattt atataaataa
     2161 gctaagatga ttgatataaa gttactgata attttaaggt aatatatacg ttactataat
     2221 attatgttat tatttgtaaa taaggggata atttataagc aaattaaaga ccttaattta
     2281 caggaggaat agtgtatatt atgaataaaa aattcaaaaa attcagtaaa aaactaagta
     2341 tattaatttt agttttgatt atgtttcttt ttaataattt aactgcaaat gcacagatta
     2401 aagaagattc aataatagga attgatagat atgaaacagc aggacttata gcagatagac
     2461 aaacttatga tactgtaata ttagttaatg gagataaaaa tttatctgat gggctttcat
     2521 caagtggatt agctggagct attaacgcac caatactttt gactaaaaaa agtgaaatac
     2581 caaaagctac tttaaataga ctagataata agagtttaaa tgttgtaaaa aaagtatata
     2641 taattggtgg ttataatact attgaaaatt cagtagaaaa agatataaaa agaaaaggta
     2701 ttcaagtgga aagaataaat ggtaacaata gaattgaaac tagttataat gtagctaaaa
     2761 aaataaatga agtaagtagt gtaaaagagg taatgcttac aaatggattt gtaggagagg
     2821 ctgatgctat gagtatagca cctgtagttg caaaaaataa aggagctata atacttacag
     2881 atgggaaatc agttccattt aagactgaaa gtttaaatgt ttacgctata ggtggcaaat
     2941 ctgcgataaa tgaggattta gtaaatgaaa ctaaagcaac tagaatcgga ggaaatgata
     3001 gatttgagac caataaaaag gtaatggaaa agttttataa tggagcgaca gatttttata
     3061 taacaaatgg atatcaatta gtagatgcac taacattatc tcctttagcc aaagaaaaac
     3121 caatcgtttt agttgctgat ggaagtaata aaggtatttt aaaaggagct aaaagtataa
     3181 caaaagttgg aggtattgat gccaatatat ataaacagtg tttagatatg attaaatata
     3241 ataatatgga tatgactcca aatattttaa aacacttaac ttcagttgaa ggaaatgaag
     3301 tttctgaaat acttatagaa tcagataatt taggtgtggt tgttgaaaag acgaatacca
     3361 ataaatttga gtttgattac gtaagtgtaa ctaatgaaaa aaattgtact ttttctgtat
     3421 ataaagaaaa ttcttctaat aatataaaaa atggaaagct agttgtaagt gctaagaaaa
     3481 aaatagataa gaaagatagt gatttgcaag atatgaatgg cgacaatatg ataaatgcaa
     3541 ataaagatag gatggtaaat gtgataaaaa ttggagttcc cgataaaatg tattctaatt
     3601 ttaaagtaga tgttgaaagt ggttcagtag aactttataa tattaaagga ggagctactg
     3661 ttgatgtcaa tgacgggatt gctaacattg ttgataacaa tgtaacaaat cctttcaata
     3721 taaatactaa tagtgggatt tcaggtgtaa cagctgaaac tatatcatct gaaataaaat
     3781 ttagagcaaa caatggaatt gtaaatgtaa cagctacgaa tataaatggc aatataagtt
     3841 tatttggaag cgataataaa ggtacctttg atggaatatt taaattaaat ctgaaaaaag
     3901 aaccatcaaa tttacacttg aagttaattg gaaatgggat aaataaattg ccagaaggtt
     3961 ggtctaaaga ttatatttta ggaaatggac aaccaattat tgaagttaaa aataatggca
     4021 taaataatat atcaattgga gaataaaaac atatgaaaat aaagtttata tagtttta
//
LOCUS       DAMJYX010000191         3872 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJYX010000191 DAMJYX010000000
VERSION     DAMJYX010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.196"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(123..1946)
                     /locus_tag="PC479_003469"
     CDS             complement(123..1946)
                     /locus_tag="PC479_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805982.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(1959..3734)
                     /locus_tag="PC479_003470"
     CDS             complement(1959..3734)
                     /locus_tag="PC479_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2805983.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1283 a          652 c          338 g         1599 t
ORIGIN      
        1 ttcataatta ctttcctcct aaattttata tatcaaatta aattttttat ttattaagtt
       61 agtctatata tacccgcacc atataccttt aaacaatttt tttaaaagtt ttttctaaat
      121 acttattttc cctgtgtcaa taatatgttt ctataataat gtccctttaa ttttatcaat
      181 tctttatgat ttcctgattc cacaattcta ccattttcta aaactattat attatcacaa
      241 tttgatatgg tacttaatct atgagctata attatacttg tctttccata agttaaatta
      301 tttattgctt cttgaatttt agcttctgtt gctatatcta tgttacttgt agcctcatcc
      361 aaaataagta ttggagcatt ttttaatact gctctagcaa tacttattaa ttgtttttga
      421 ccttcactta aatcttcacc cctatccgat aatacagtac tatatccttc acgcatttta
      481 actataaatt tatgtgcatt tgctatttta ctagcatata taacttcttc atcagtagcc
      541 tccagatttc cataccttat attttccata actgtatcat tgaatatata accttcttgt
      601 ggaactactc caaaacaagt tctcaaatag tttctgttaa tagagtatat atccttacca
      661 tctaaaagta tacttccttc atctatatga taaaaatttg taattagatt tataatagtt
      721 gtttttccac ttcccgtctc tcctatgatg gcatttgatg tgccagcttt gatttttagg
      781 tttatattat ctaaaatttt cttttttgaa tcataagaaa aacttacatc tttaagctca
      841 atttctccct ttacaaaatg agagtttaaa ttaccatctt tattatcgtt aatctcactt
      901 ttaaacatgt tatgcacttc ttgctcttct tgttcatcta taatttcaaa aactctttca
      961 catccagcta tggctgaaag taatgtatta aatagatttg ctaattcatt taaaggtctt
     1021 gtaaattgtc ttacataact taaaaaacta gctataaccc ctatggttat aatatcttta
     1081 gatgcaagaa tgcctcctat aatacatata gttgcaaatc ctatattact aatcacattc
     1141 ataataggca ttaaaagaga agtgtaaatt tgcgatttaa gggaaatatt tagaattcta
     1201 tcattgactt ctttaaaatc atttacagat tttctttcat aattaaaaga ttttactaca
     1261 tttatgtttg atatcatttc ttcaatatgt gcatttagtt ttcccaactc aacttgttgt
     1321 tgcttaaaaa aaactctagt tctctttgca ataaaattac tcacaaacat tactatcggt
     1381 attaatatta cactgcaaaa ggttaaaatt gggcttaata caaacatcat aataaaagtc
     1441 atcaatatgt ttaagattcc tgtaattaac tgtactaatg aatttgatat tcctgtactg
     1501 atattatcaa catcatttgt cactctactc ataatatctc cagtagaact tttatcaaaa
     1561 aaggttattg gtagattttt aaattttaaa aataaattac ttctcatttc tttaactata
     1621 ctttgagata atctagctgt acatatcccc ttagacagtt taactgaaaa ctcaattata
     1681 taaaatgaaa gtaaaataat tattcctttt aagaaaatgg attttgaata agtatcgata
     1741 tctattatgt caactacctt tccaatagta tatggtatta cagttgatat aattgaatca
     1801 attattacta gcaaaaaaat aaatctagta atcttcttat cttttttaaa gtacataaaa
     1861 attcttttaa acgttttttt aaaatcttta ggcttttctt ttttctttaa tctactgacc
     1921 ttctgatttc cagtacttaa attcaatata cttcctcctt aatttacatc ttcacttact
     1981 aaattctgca acgcaaaaat ttttttataa attggagaac taaccagtaa atcttcatgt
     2041 gtagcaaatc caaccacctc accctcatcc aagactaaaa tcttatcaca gtcaattata
     2101 gaactaatct tttgtgaaat agttattaca gtacatttct tactttcctt ttctcttaaa
     2161 ttacttatat actcttttat attctttctt ataattgtct ctgtattaat atccattgca
     2221 ctaaaacaat catcaagaat taatatttgt ggttttttgg ctatagctct agctatagta
     2281 attctttgtc tctgtcctcc tgataaattt ttaccccttt ggaataaaac ttcatcataa
     2341 gtattttcga gttttgttat aaattgattt gcatttgcta tttcacatga ttttacaaca
     2401 tcctctttgc atatatctct atttcccata actacattct ccaatatact ttttccaaac
     2461 acactactat tttgaaatac tacacctatt cttgccctta aactttcttc agaaatactg
     2521 tatatactac gtcctccaat cttaatatca cctttatcaa ttatatataa tttttgaatt
     2581 aaatttacta aagaagtctt tcctgaacca gtttgaccaa tcacaccaat attttgacct
     2641 tcttctattt taaaggatac gttttttaat attttttcat ctgtattttt gttatacgaa
     2701 aaatctacat tttcaaaaat tatatcacag tttacaaact ttaaatcttt ttctccaaca
     2761 tctaaacttt cttctctctc aattacttct tcattctcat caaccaaaac ctcacttatt
     2821 ctctcataag atgcttttgc cctaacaaaa acattaaaaa catgtgaaat cattataaga
     2881 gatgttaata ttctagtcat atagtttaca taagcaacta taatcccaat tttaatttct
     2941 cctgaataaa ttaaatgcct tccaattagt aatattatta ttatggaaac attaatgatt
     3001 attgtaattg tcggtctaaa aaatgacatc atagttgtag cttttctact atttttaaaa
     3061 agttcatcat ttaactcttc aaagctttgg ttctcataat cgtatctaca aaatagttta
     3121 actactttta ttccacttaa aaactgtctt aattttgaat ttactttatc tgtactttct
     3181 tgtacatttt taaataatct ataccctatc ttcatattaa gcaatattat aataaatata
     3241 attaaaactg caattagaat aattattgac atctttaaat ttaattttat agctataatt
     3301 aaacttccta tacacattat tggtgacttt ataaaaactc tcatcattcc attaacgaat
     3361 tgttgcattt gattgatatc attagtaatt cttgttatta atgaagctct accaaattta
     3421 tctatttgtt ttaatgagta tgtatttatc ttttcaaata catcctgtct taaatcataa
     3481 gaaaaatgct gagaaactct tgccgagatt acacttctta ttattgcaaa tattgcacct
     3541 aaaaaagtta tacctatcat tatagctcct gttgatagta tgtagtctaa gttcttattt
     3601 gaaaccccta tatctattat ttttgccatt atagttggtt gagctaaatc acaaaaagct
     3661 tccaatgtta aaaaaaatac tgctgttaga aataatacta tatatttttt tatgtatttt
     3721 ttaaaatacc tcattcaatc atcccttcat agaattttct cattaccaaa agtatatcat
     3781 aatggctaat ttaattgatt atatcaatta taaatatttg atttattatt aatttttata
     3841 ccatatattt tataaattat ttgtttttat tt
//
LOCUS       DAMJYX010000192         3817 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJYX010000192 DAMJYX010000000
VERSION     DAMJYX010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.197"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            108..782
                     /gene="fsa"
                     /locus_tag="PC479_003471"
     CDS             108..782
                     /gene="fsa"
                     /locus_tag="PC479_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2805984.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(904..2298)
                     /locus_tag="PC479_003472"
     CDS             complement(904..2298)
                     /locus_tag="PC479_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2805985.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(2376..3722)
                     /locus_tag="PC479_003473"
     CDS             complement(2376..3722)
                     /locus_tag="PC479_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2805986.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         1513 a          534 c          531 g         1239 t
ORIGIN      
        1 gaaaagtaaa acaataataa aaaataaaaa acaataagca aaatgttgaa aataatacca
       61 aatcaatata taataagaat aaagaaagat taaaggggga tattttaatg caactaatct
      121 tagatacagg taatgtggag gagattaagg agttatgtac ttgtttacca atagatggtg
      181 taactacaaa tccaagtatt gtttcaaaag aaaaaaagaa ttttaagcaa cttataaatg
      241 aaattggtga gattataggt gaggatatgc ctatacatgc tcaagtgtta agtacaaagt
      301 atgaagaaat attagaagaa gcattatata taagtagttt gagaaaaaat atatatgtaa
      361 aaatacctgt aacacaagat ggtttaagag ctataaaaga ccttcacaga aaaggtgtaa
      421 aaataactgc tactgcaata ttcactgctc atcaaggttt tttagctgca aaggctggag
      481 caaattatat tgcaccttat gtaaataggc ttgataatat atctggtgat ggagtagcta
      541 tggtttcaga acttataaaa ataatagata cttataaaat ggatacaaaa gtacttgcag
      601 ccagttttaa aaatgcacaa caagttatag aattaatgca acatggggtg catagtgtta
      661 cagtaccata tgatatttgt aaatctatga tgaatcatcc attaactgat tggagtgtag
      721 gtaagtttat agaggattgg gagaatactt ttggaaaagg aagtaaaaca aataatattt
      781 agtattatgg taaaagagta gttttgtcca atttcacaag aacttatata aaaagatgaa
      841 aatatgaatt ttctttttaa taatttcgtt attatcttaa gttaattcat attttcatct
      901 tttttagatt tctagtccta taaatttcca ccaagggaat gttatacata ttattataaa
      961 gtaggttaaa ggaatcaaag ttaaaccgta tttgaaaata accttgctat tatacagatt
     1021 ttcaccatga ccaaccatta ttgtagcatg atgaaatggt aatatgtact gtatgcttac
     1081 tataatgtag acaaatagac ataaagtaac actattttcc tttagtatat gtaaacttcc
     1141 aagtaacggt attactacag atgatgtagt gacagcactt ccaagacata tattcaagat
     1201 tattgtgaga gttatcaaaa acaatattga aataatttct gaagagtttg gtataaactt
     1261 tatcaaataa tttcccatta tatctacaac tccagagtac tttaacactc ctccaattga
     1321 aaatgctgct ataaaaaaaa taaggacctt ccaatttatt tcttttaaag tactaaatcc
     1381 aactattttt cttagtaata aaagaagtac acaaattgac attataatta atgtgtttat
     1441 gttatgtatg aactgagtta aaaacataaa aaacattatt cctaggcata aaaatacgtg
     1501 tttttgctct ttgtttagca aattaaattc tttttttaaa ttagaatatg aaaaatgaag
     1561 tttattaccc tcaagatttt cattagaaat aaattttatt gaatgcaatt cttttttaaa
     1621 gacaaagata aataaaaaat aagttataaa acaagtaatt atagttggaa cagacatata
     1681 aaaagcccat tgaccccaat ttatattaca atttccaagt tctagtacaa cataatttag
     1741 aagggtgtct ccatttacaa aaaacattga tgtaaaggta gatgctgtaa atatactaaa
     1801 taaaagtata gattttgaat cttcagttat ctgttgttct tttaaaaact ccttgtaaaa
     1861 agcagataca agaataaccc ttggaaatgg ttgtgggata aaaaatatta aaagtatacc
     1921 taaaatatac gataaaagaa tcattttttt aggggtattt acagtattta aaattaactt
     1981 ctcagaaata atttttgcga cacctgtttt tgtaactgca ttagtaatta tgtatgcaag
     2041 aattattatg taaaaatttt ctgtaaataa aaactgtaaa ataaccttta ttggtgcaat
     2101 gttaaatata aacgctagaa ttacaaaaaa caatgcaaca aaagtttttt caactgagtc
     2161 tacagcccat atcaagatag ttaaaaaaga taagcatata gaaaatttta tgttgaaact
     2221 gtaattaaga gagaagtaga gaattatggt agctataaca gtcatataaa attgttttgg
     2281 cttaagaata atacccattt ttatattcac ctccataatt tattatatca taatggaggt
     2341 gaatacaatg aatgtgaata cattttataa aaagattatt cattgattct atgatttaca
     2401 atagctttag ttttccaatg tcctttctta tagtagataa ctgtaacaat aagacctaaa
     2461 gtccaaccaa aaggaatacc catccaaatt ccgtctgttc caaaaaattt agaaagcata
     2521 taagaagctg gaagtcttcc aagccataaa gatactattg agcttataag tgcaaacatg
     2581 gaatcaccag cacctttcat tgcacttgaa agtacaaaag aagttccaat aaataagaag
     2641 aatggtccta ttataaataa gtaagaagag ccaattttaa tgacatcttt atcagtattg
     2701 aatagagcaa ttagattttc cctaaatagc aaaattaaag caataactgt aactgctaaa
     2761 actaaggtca tttttataga ttctcgaatg ccttttttaa ctctttcatt ttctccagca
     2821 cctatgtttt gagcaacaaa agtagaaaca gcagaaccaa gattcataat aggtaagctt
     2881 ataaaatttt ctatcctaag accagcacca aaagctgcca tagcagatgt accataacta
     2941 ttaactaaaa attgaagagc catatttcca attgaaaaca acatttgttg aactccactt
     3001 ggaagtccta atctaagtcc atctttaaat attttgttat catatacaat tcctttgatt
     3061 ttaaatttaa gcacttcatg ttttttattt aaataaatta tacttattat aaatgatact
     3121 gcctgagcca ttatagtggc aagtgcaacg ccctcaacac ccattcttag cactacaata
     3181 aaagttaagt ctaaaactat atttagaatt gtggctatta tcagaaaata aagtggagtt
     3241 actgaatctc caagtcctct aagtattgca cttactgaat tatatccagc agaaaataat
     3301 attccactaa atattatagt aagatacaat tttgattgag caaaaacgga ttctggtgta
     3361 ttcataagct taagcattgg gccaccaaat actattccaa gaatacttat taatatagaa
     3421 ccaataaata aaagtatata tgtagtatca atagtagctt ttaccttttt taaatctcct
     3481 gagccataaa attgtgacac taaaatagaa aatccaaggc taacacccat aagcgctgcc
     3541 accaataaaa acattatagg gttagctcca gagactgccg ccattgcttc ttttccgata
     3601 aatctaccaa ctattatact gtcagctgta ttgtatagtt gttgaaataa actaccaata
     3661 agcataggca tggcaaaaaa gaatatggac tttccctcat gacctgtagt taaatctttc
     3721 accttattcc tccaaataaa aaaattatta atttattata gtataatctt aacataaact
     3781 ttatatccaa actataaata aattgtatca aaatatc
//
LOCUS       DAMJYX010000193         3693 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJYX010000193 DAMJYX010000000
VERSION     DAMJYX010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.198"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            17..1564
                     /locus_tag="PC479_003474"
     CDS             17..1564
                     /locus_tag="PC479_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2805987.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            1567..1965
                     /locus_tag="PC479_003475"
     CDS             1567..1965
                     /locus_tag="PC479_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2805988.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2005..>3693
                     /locus_tag="PC479_003476"
     CDS             2005..>3693
                     /locus_tag="PC479_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2805989.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKL"
BASE COUNT         1560 a          301 c          613 g         1219 t
ORIGIN      
        1 aaatagggga atttatatga ataaaaatag catgacaaga atttacagtt taaataaaat
       61 aatttttatt ataattggaa ttttaggaat agttttactc acaagacata ttgaggtaga
      121 tattaaagtt ttaattttaa ttttattaac cttgtttagt ataaatgtat ttatttcata
      181 tcttaaaatt aagttgtatg aggataaaat taatttagga ttgataaata attcagataa
      241 gtttataaat attactaaag atgagaactt cctaaaacat gttcaaaatt atattatcag
      301 taatgaaaaa gaaaactgtg taatggcttg ttttgattta tgtagactca aacttataaa
      361 tgatatatat ggttatgagc taggggatga agtgttaaat aatattttaa gtaacttaaa
      421 aaattatttt ggaaaagaag ctatttatgg aaagttaaaa agtgatgttt ttgtattaat
      481 aataaagctt gaaaataggg aacagaagat tccttattta gtaaatctta ttaagaataa
      541 aattgtgata ctaagtcaga ttgacagttt taaaatggat ataaatattg aggcttctat
      601 tggaatttat aaatttaaca ataatgaaat tgatataaaa aaagctatag ataatgctga
      661 tatggcaaga cttaaatcaa aaggactaaa gcacatagag tatgtagaat ttgataatgc
      721 tatggaagaa gaaatacaat caattatgaa aatagagaga gatttattct tggctattaa
      781 aaataaagag tttgtaattc actatcaacc taaagttgat tcaagtacag gtaacattat
      841 aggttcagag gcattgataa gatggttgca cccatcatta ggcatggttg gaccaaataa
      901 gtttatacct atagctgaaa aaaatggtct gataaacaat attggtagat ggctaattca
      961 agaagttttt attaccatta acaagtggat ttcagaaggt ataaatgtct taccagtctc
     1021 aataaatctt tctagagtag aactatataa aaatgattta gttgattttt ttaaactgat
     1081 gtttaataca tataatattc caaaagaact tatagaatta gaaattacag aaacgacagc
     1141 actaagagat gtaaaattta taagtgaaag gctttatgag attaaatctt taggtatgaa
     1201 tatatcatta gatgattttg gagtaggtaa ttcaaatttt atcaacctta aggggatacc
     1261 actagatatt ataaaaattg ataggtcact tatattggat atagttacaa atacaaaaac
     1321 aaagtttatg gtgaaagcaa ttgttaacct ttcacatgat ttaaacgtaa ctgttatatg
     1381 tgaaggggta gaggatatgc atcaggtcaa agtcttaagt gaactgggat gtaatttcat
     1441 acaaggttat gtatttttta aacctttgga tgagatgaat tataaaaaac ttcttactga
     1501 cggaagttat ataaatctga aagatactct tttagataaa tgcatgacag ttgaggagga
     1561 gtaaggatga tttcagaaga tgtagattat tcaataagaa tcataagagc tatatttaat
     1621 aatggagaag ataaaccaat agatgctaaa atgatattaa aaagtgaaga tataccaata
     1681 ctaacactat acagactgct tagaaagctt tgtgcaaaca atattcttac ttttaacaaa
     1741 gaagatggca agacatacat gttaaatgtg tcattggata caataagtct aagaactatt
     1801 gtagaagtaa ttgatggaaa aatatatata aataaatgta cgagatatgt atctaattgc
     1861 agtaattgct taaaaggttg taaagtaaat cgtgaattgc tagaaataga aaaagaaatt
     1921 tataaacttt tatcgagaaa atctataaga caaatactag aataaaaaat tttcagctat
     1981 gataataatt ggaggttttc ggttatggat gatatatctc aagataattt tctactgtct
     2041 aaagagtatg agaatagctt agatgtagat actaaaaaag caagtggaat atattatact
     2101 cctaaaataa ttgtagatta tattgtaaaa aaaactttaa aaaatcacga tataattaaa
     2161 aatccatatc cacgaatact tgatatttcc tgtgggtgtg gaaattttct tttagaagta
     2221 tatgatattt tatatgactt atttgaggaa aatatatatg aattgaaaaa gaagtatgat
     2281 gaaaattatt ggactgttga taacatacac cgtcatatat taaactattg tatatatggt
     2341 gctgatattg atgaaaaagc tataagtata ttaaaagata gcttaacaaa taaaaaggta
     2401 gtaaatgatt tagatgaatc tgatataaag attaatctat tttgttgtga tagtcttaaa
     2461 aagaaatggc gatataagtt tgattacata gtgggtaatc caccctatat tggtcataaa
     2521 aaacttgaaa agaaatataa aaaatttctc ctagaaaaat atagtgaagt ttataaggat
     2581 aaagctgact tatatttttg tttttataaa aagattatag atattttaaa gcaaggaggg
     2641 attggcagtg ttataactcc aagatatttt ttagaaagtc tatcaggtaa agatttaaga
     2701 gaatatatta aatcaaatgt caatgttcaa gaaatagtgg attttttagg tgcaaatata
     2761 tttaaaaata taggggtatc tagttgtatt ctaacttttg ataagaaaaa aacaaaagag
     2821 acatatatag atgtatttaa aatcaaaaat gaggatatat gtataaataa atttgaaaca
     2881 ttggaagaac ttctaaagag tagcaaattt gaacatttta atataaatca gagattgcta
     2941 tctgacgaat ggatacttgt aaataaagat gatgaaactt tttataataa gatacaagaa
     3001 aaatgtaaat atagcttgga agatatagca ataagttttc aaggaataat tacagggtgt
     3061 gataaagcgt ttattttaag taaagatgat gtaaagttga accttgtaga tgataaattt
     3121 ttaaaatgtt ggataaaaag taagaatata aataaatata tagtagataa aagtgaatat
     3181 aggctaatat actcaaatga tatagataat gagaatacaa ataaaaggat actagatgaa
     3241 attatagggc tatataaaac aaaattagaa aacagaagag aatgtaaaag tggaataagg
     3301 aaatggtatg aacttcaatg gggtagagaa aagcttttct ttgaaagaaa aaagataatg
     3361 tatccttata aatcaaatga aaatagattt gctatagatt atgataataa tttttcaagt
     3421 gcagatgtat attctttttt tataaaagaa gagtatttag ataaattttc atatgaatat
     3481 ctagttggaa ttttaaattc cagtgtttat gataaatact ttaaaataac tgctaaaaaa
     3541 atgagtaaaa atatatatga ttactatcct aataaggtta tgaagataag gatttttaga
     3601 gataataatt atgaagagat agagaattta tcaaagcaaa taatatcaat tttattaaat
     3661 aagtctatag ataaaggaaa agtagaaaaa ttg
//
LOCUS       DAMJYX010000194         3578 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJYX010000194 DAMJYX010000000
VERSION     DAMJYX010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.199"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            119..1495
                     /locus_tag="PC479_003477"
     CDS             119..1495
                     /locus_tag="PC479_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2805990.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1806..2477
                     /locus_tag="PC479_003478"
     CDS             1806..2477
                     /locus_tag="PC479_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2805991.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2666..3463
                     /locus_tag="PC479_003479"
     CDS             2666..3463
                     /locus_tag="PC479_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2805992.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1345 a          399 c          671 g         1163 t
ORIGIN      
        1 attgtcttta tacaatattt atactataat agaataataa tttagaggaa agctctaaaa
       61 ataaacaaat taagggtctt aacagtagtg attttatgtt aaggaggtat aaagaaagtt
      121 gaaaacaata gttagaagac ttattctcta cgcagatgga atgcgtccta ctcaaataat
      181 ggtatctggg tttgctgcga ttattgtaat cggagcattg ttgttaacac tcccgatagc
      241 gtcgcagagt ggagagagta taggcttatt aaatgctcta tttacggcta cttcagcggt
      301 ttgtgttact ggtctagtta tggtagatac agcaacttat tggagtttat ttggacagat
      361 agttataata acattgatac aaattggtgg tcttggattt atgaccgtag ccacaatgtt
      421 ttcacttatg gcacgaaaaa aaatacaact aagagaaaga ttattaatac aagagtcatt
      481 aaatcaagct gatttatcgg gacttgtaag acttacaaga tttgtattaa taataaccat
      541 aacaatagag ggaataggtg cactggtttt atcaactgtc tttattccac aatttggttt
      601 gtctaaaggg atatggtata gtgtatttca cgcaatatca gcgttttgta atgccggatt
      661 tgacttgatg ggcagtgtaa gtggcccatt tacatcgtta aattcttatg taaacaactt
      721 tacagtatca atgactgttt gcgcacttat agtgcttgga gggctaggat tccctgttgt
      781 acttgatatt gtaagaaaaa gaagattttc aaaattaaat gtgcattcta aggttgtatt
      841 gttttcaact gctacactta tttttgtggg tgcattattt atttttctta tagaatttaa
      901 tcaaaaagct actatggcgg acttgccttt aaagggaaaa gtactatcag caatatttca
      961 atcagtaaca gcaagaactg ctggttttaa tacgcttgat ttagctactt tacgagaaag
     1021 tagtgtattt gtaatgataa ttttgatgtt tattggagca tcacctgcat caactggtgg
     1081 gggtataaaa acaacaacac tagctgtttt aatcattaca gttagaagtt ttctatcagg
     1141 aaaatcagat atagaagctt ttgaaagaag attagctcca tcaactataa aaaaatctct
     1201 tggtatattt gtaatcagta tatcagcagt tatttttgga acattgatta tttcaataac
     1261 tcagcctaat tttacattag ttcaatcagc ttttgaggtt acatcagcac ttgctacagt
     1321 tggttcaagt ttagctggaa ctccaaactt aaatgcttta ggtaagatta taataataat
     1381 ctttatgttt atgggtagag ttggttctct tactttattt atggctatac tttctggtgg
     1441 tagaagaaag agtcaaccaa tcagatatgc agaaggtaaa attatggttg gttaagttac
     1501 caaggagtag tataaattta taatgggttt agagtagcac tgtaaatctg ttgataaaat
     1561 aaatgagcct agatgtaatt atatgaagaa ataaaatcaa ctaatgaaca aaattaatta
     1621 atgaaataaa aaaacaggga aataaaatac aaataaaaag gtaaatcaaa attaaattaa
     1681 tctgtgtgta taaaattata tattgtgtat aagttttgta tgtgtaaagt atataagttt
     1741 tgtatgtgta aacaattaat ttaagagata ttatataaat tgtgtattag ggaggaagtt
     1801 tcgagatgaa gcaatatata gtcattgggt gtgggagatt tggaagttca gttgcgtcta
     1861 ctatgcatct tttaggacat caagtaatgg caatagacaa aaatgaagat tcagttcaaa
     1921 gtatatctga caaggtaacc cattcactta tagtggatgt tactgatgag caagcgttaa
     1981 ggtcattagg tttaggtaac tttgatgtag cagtagttgc aataggttct gatataaggg
     2041 catctataat ggcgactctt atagccaaag aaatgggtgt agagttgata atatgtaagg
     2101 caaaggatga attacaagct aaagttcttt ataaaattgg tgcagataga gttgtatttc
     2161 cagaaagaga tatgggagta agagttgcac acaatttagt ttcggataat atattagacc
     2221 atattgaact tgacccagag tattcaattg ttgaaatcgt aactccaaat agttgggttg
     2281 gcaagacact tatagagctt gaattaagag ctagatatga gataactgta cttgctataa
     2341 aaacaggtaa aaatataaat gttacacctt ctccagatga ggaacttaca gccggaagta
     2401 tcctagttat aatcggtcaa aatactagta taacagcgat aacatctgga aataagggga
     2461 taattagaag aagataattt actatttaat atatatttaa ttgcaatgaa agcagatagt
     2521 atcatataat tatgaatagt tatatgatac tattttttta atcgaaggta gtatttattt
     2581 gtaagattag ataaagagaa gttaaattaa aagtaaggag gctgtgctaa tataaaaatt
     2641 tataagttat tagcatttag ataaaatgat tacaaatata aacagtaagg ataatgaaaa
     2701 gttaaagtat acaagagcac tattaaaatc aaagaatagg aataaagagt caaagttcat
     2761 aatagaagga tacagaatag taatgcttgc acttgaatgt atggcaaacc ttgattatgt
     2821 atttatcaat gaagaatttg aaaataagaa agaacatgta aaactattag aagatttgga
     2881 taaaaaaaac acaaagatat acaagactac taataaaaac tttaaagaat tagtggatac
     2941 agaaaatact caaggaataa taggtgtagt ttcatttaag aaaaaaaaat taagtgaaag
     3001 tataaataaa aaagataaat ttgtattgat tttagacaga atacaagacc caggaaatat
     3061 ggggactata ataaggactg ctgattctgc tggagtagat gccataatag cactaaaggg
     3121 atgtgtcgat atatacaacc caaaagtaat taggtctact atgggttcta tttttgatat
     3181 gaatataatt gatgcttcac aagatgaaac tgtggacatg cttaaatcat tggattttaa
     3241 tatagtttca agttacttaa atacagaaaa tttttatgac aaaatagatt atggctcaaa
     3301 agtagcattg gtgataggaa acgaagcaaa tggaataaat gaagaacttg tatcaaagtc
     3361 tgatattttg gttaagatac ctatatatgg taaagccgag tcgttaaatg ctgcgataag
     3421 ctctgctata ctgatgtatg aaataaaaaa atacttaatt taatgtattg taataaatat
     3481 aatgtcatgt tataatcttg aattaaatag tagagtatca aaaatagtca atatatatta
     3541 aaaaaataat taaattaatt atatattaga tgtatatt
//
LOCUS       DAMJYX010000195         3542 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJYX010000195 DAMJYX010000000
VERSION     DAMJYX010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.200"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            79..1659
                     /locus_tag="PC479_003480"
     CDS             79..1659
                     /locus_tag="PC479_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2805993.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1833..2279
                     /locus_tag="PC479_003481"
     CDS             1833..2279
                     /locus_tag="PC479_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2805994.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2300..3343
                     /locus_tag="PC479_003482"
     CDS             2300..3343
                     /locus_tag="PC479_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2805995.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1426 a          331 c          685 g         1100 t
ORIGIN      
        1 ttatgggatt aaaaagattt caatagatat ttttggtaag tatttaagaa gtctttaact
       61 ttataggagt gataatttat gctaataggt acatctaaat taagcagaaa tgttgatact
      121 gcatgtgtag aagaagtagg gattccaatg attgtattga tggaaaatgc ggttatatct
      181 gcaatgaaaa atatggatat agacatgtat aatagttata cagttgtatg tggtgttgga
      241 aataatggag gagatggtct tggaatagca agacatctaa atacatatgg aaaagaagta
      301 aacgtatttt tagtaggtaa tcttgaaaaa ctaagtgaat gctctaaaat aaattacaat
      361 gtacttttaa atatgggaat taatattata aatattaata aaaatgattt aagtgaaaaa
      421 aattatggag aggaaaatga agtagataaa attattaaag ttgagaaaca aaatattata
      481 aaaaataaaa atttaacaat ttttaaagat agagtatcta aaagtgatgt tgtagtagat
      541 gctatatttg ggactggatt aaaacgtgat ataactggta tttttaaaga agttattgac
      601 atagtaaacg ataatagcaa aaatacgtat tctatagata ttccttcagg tatagatagc
      661 gataatggaa atgtattagg aacttgtatt agagcaaaca aaacgattag ctttgagttt
      721 tataaaagag gttttctaaa ctatgacact agcgcactta taggtgatgt agttgtagaa
      781 aagataggtg ttccagattt tattacaaga aaataccatg atgaagagtt cataactgat
      841 aagacttttg taaaaaataa tattagaaga agagataagc atggttttaa aagtgatttt
      901 ggaaaagtat ctatagttgc aggttcaaaa ggattttatg gtgcaagttt catagcaact
      961 gaatctgctg ttaagagtgg gagtggactt gtaacattaa ttagtagtga agatgtgcaa
     1021 gaaaaggtat ctattagact tacagaagca atgactttaa attttgaaga agaaagatta
     1081 aataaattac ttaattctag tgatgcaatt ggttttggtc caggtatggg agataatagt
     1141 caaacatttg ataaattact taagatagta gaaaattcta attgcccaat agtattagat
     1201 gcagatggat tgaacgttat gaaggacaga tgttataagt ttttagaatg gaaaaataga
     1261 tttgtaatta cacctcattt gggagaaatg gccaggttga caggagattc aattgggtat
     1321 ataagagaac atagagtaga tgttgcaaaa gaatttgctc aaaaatataa tctggtggtg
     1381 cttttaaaag gttatcaaac tgtcataaca gatggtaaaa aaacatatat aaatcctaca
     1441 ggaaatagct gtatggcaac tggtggaatg ggtgattgtt tgcttggaat gattacttca
     1501 tttataggtc aaggtatgga tatattggaa gctacagtta gtggagcata tatacatggc
     1561 tatataggag ataaattgag caaagcaatg tatacggtaa atgctacgga tttaataagt
     1621 aacatttcat taacaatgaa agagttgttg gatatataat tggtgatatt tgatactact
     1681 ttgtaacaat tttaacaaag tatgttgata taatactatt aattaattaa tttattttat
     1741 tgttttgtaa tttaaattta tattcaaaaa cattgtataa acatataatt tatatagggt
     1801 tttataattc atatgaaaag agggtagata gtatgaagct aatatggttg atagtagcag
     1861 tcttatttgg aatagcagaa atgttaacac caagcttaac attgatatgg tttagtgtag
     1921 gagctgtaat acttatattt ttaagtagct ttatagagag tattttctta caaattttaa
     1981 tatttgcagt aatttctata gcaatgttag tcgtagctac taaaaaaata gtaaaaaaag
     2041 ataaaggcta taaatctaac actaatttac aagcaatgat gtctaaaaaa ggtattgtta
     2101 cagaagagat ttctccaaat aacacaggtc tagttgtagt tgaacatgaa acatggacgg
     2161 ctatttctat agatggtgaa aagatagaaa aaggttctac agtagaagta ttaaaaattg
     2221 aaggggtaaa attagttgtt aaaaaagtag acgccacagt taatgtgaca aaccaataga
     2281 ataaaggagg atttatagta tgggtactaa aattgtttta agtattgtat taatagtagt
     2341 agtcgtagca ataagtttaa cttgtataag agttataaaa caatctaaag taggtataat
     2401 aatgagactt ggtaagtttc aaaaagtggc tgaaacaggt gtacatttct taataccatt
     2461 tttagataaa atggcatatg taattgacct tagagaaatt gtaatagatt ttccacctca
     2521 accagttata actaaagata atgtaactat gcagattgat acagttgtat attataaagt
     2581 tacagaccca gttagatatg tgtttgagat agctaatcca atagctgcta ttgaaaattt
     2641 aacagctaca acactaagaa atataattgg tgaacttgat ttagatgaaa cattaacatc
     2701 aagagatata ataaatgtaa aaatgagaac tattcttgat gaagcaacag ataaatgggg
     2761 tataaaagta aatagagtag agttaaaaaa tataatgcct cctcaggata ttcaagttgc
     2821 aatggaaaaa caaatgagag cagaaagaga aagaagagag gcaatacttc aagcagaagg
     2881 taataagtca gctgcaatac ttcaagctga aggtgaaaaa caatctgcta tattaacagc
     2941 agaagccaaa aaagaagcta tggtacgtgt agcagaaggt gaaaaagaat ctgctatatt
     3001 agtggcagaa ggtgaagctg aagctataag acagacagct attgctaagg cacaaggtga
     3061 agctgaaatg ataaaaagaa ctcaaatggc aacagcagaa ggtttaaaat tagttttttc
     3121 agcaatgaaa gaggctgata tagacaataa tattctagca ttaaaatcta tggaagctct
     3181 tgaaaaaatg gctgaaggta agtcaacaaa actggtttta ccttcagaag cagttaattt
     3241 cctaggaaca ttcaaaggaa taaaggaagt tatgagtgat gataacaaag aagtacttga
     3301 tataaaagaa gttttaaatg ataatgaatc attaaaaaaa taaaagagtt ttgttatcat
     3361 aggaaggata tggaaattaa aggtcgaatt aattaaatat agcacaaaaa tgcacgttga
     3421 aaagaagagg acttataaat ttttaagtct tcttctttgt ttttattaga aagatgaaat
     3481 tataaaaata aattaacaga atttttatac aatttatcct ttactaatgt aaaaagtgtt
     3541 at
//
LOCUS       DAMJYX010000196         3277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJYX010000196 DAMJYX010000000
VERSION     DAMJYX010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.201"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            210..1577
                     /locus_tag="PC479_003483"
     CDS             210..1577
                     /locus_tag="PC479_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2805996.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1602..2210
                     /locus_tag="PC479_003484"
     CDS             1602..2210
                     /locus_tag="PC479_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2805997.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2258..2629
                     /locus_tag="PC479_003485"
     CDS             2258..2629
                     /locus_tag="PC479_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2805998.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2808..3146
                     /locus_tag="PC479_003486"
     CDS             2808..3146
                     /locus_tag="PC479_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2805999.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         1403 a          241 c          573 g         1060 t
ORIGIN      
        1 tttaataact ctcatttcat atgtaaagtt ggtaaaaaaa ttaaaattaa aagtaaaaaa
       61 ctaaataaat taatgttaat ttgaaatata tatgaaggtt tttggagaat acttacttat
      121 gataggaaaa aatgttaaaa taatagtgta atcaaatata aaggactgaa ataattaaca
      181 gttattaaat attaggagtg taaaaattta tgggattaaa agaatttgat tttattgaag
      241 agtctattga tatgttgagg acaatgtcac caacattaga aactatatca gatgaaatag
      301 aagaatattt tgaaaatatc ttggatgaaa agaatcaaga atatataaat gtaacctcta
      361 gaataaaatc agaatcaagt ttaagagaaa agataattag aaatagatat ttaaaaaaat
      421 atggagaagc aagtaatctt attcataatg tttctgactt aataggccta agaattgagt
      481 gtagatttat tgaggatgaa aacaaaatat atagactttt gagaagatat tttaataaaa
      541 cagatgacaa aataaactat tataataaag agaataagaa tattaaatta aaactatctg
      601 agaggcaacc caataagcaa aaaaatggat ttgaaatata taaaatagat ggagtttttg
      661 cgtatttaga tagagaagta aaatttgaac ttcaaattaa atctcttgta aatgtgtttt
      721 ggagtgaaat agaacataaa ataatttata aaaacaatac atatttactt gctgataaat
      781 ttataaagga tatgatggac tctataaaga ataatcttac tatgatagat aatcagcttt
      841 taagtattta taaaaacttt cattcaggga agtcttttaa tatgaaagta agtaaaaaag
      901 aaattgaaaa attatttgcg aaactagtct atgacgcttt ttctgaaaag atgaataaaa
      961 gtataggttt tgttgtagat tttaaaaaac catgtgagac tatattgaac tattctttta
     1021 ataaacaaga tataagtgat gaagttttag gtaattttat gcttgatgag tttgcaagat
     1081 taaatgaaat tgtaaacaag gatatagatt ttaatgagca aatagaattt gaaagagaac
     1141 caacatttga tgataagttt tgtaaagacc taggaaatca ttttagaagt agattaaaca
     1201 cagaatttcc atggaaccta ttttttagaa tattatttga aatagagcca tataacaata
     1261 ccaaagactt tgaaaacttt gtagagttta taaaagaaaa tgtgctacta gaagaaaata
     1321 gggagcaatt attatgccaa tttgaagagg atagtaagct tataattgaa gatatatatg
     1381 attgtatatt taatagtata agtgagattg atagtgttga aattttatat aactataatc
     1441 ttgaaaaaat aaactttaca tcaagtgaaa tcataaattg tatatgtaga gaatatgagt
     1501 gttatggtga atacttagaa gaaaaagaaa agcttatgaa tacatttaaa gaaaagatta
     1561 ttcaaatctt tgagtaatag gtcaatgctg aagtggcgct aatgcgccat ttttcaaata
     1621 tatatgaatt aagtaaaatt attttggagg aggcgatttg tatagagaaa acaggtgtaa
     1681 ttttggcagg gggaaaaaat tctagaatgg gtagagataa agccttttta gagttacatg
     1741 ataagctttt tattgaaatt gcaatagaag catttaaaaa ctttgatgag ttgataataa
     1801 tatctaacaa tgaagaatta tattctaaat acgatattaa agtttataat gatatagtaa
     1861 aagatgttgg tccaattgga ggaatatata ctgctcttga atatgcaaag tatgatatag
     1921 ttacaattgc atgtgatatg ccatatctaa ataatcaaat agtcgaaaga atagctaata
     1981 aaatggatga taaaagtgtg atttcagtga caaatggaaa gttacaacca ttgtgttctg
     2041 gatataaaaa gagtataata aataaagtat ctttatgtat aagggaaaat gatttaaagc
     2101 tcagaagctt tatagataaa atagataaaa gttatatata ctttgatgaa gaagatttat
     2161 ttttaaatgt aaacactgtt gatgagtata aaaaattaac taaagtttaa ttagctatgg
     2221 ttgtaaaata gagtaggatt tatatggagg agagtttatg aatttactta aatgcgattc
     2281 ttgggcagta atattaaata atagcgataa agaaagtaaa gcatataaaa tattagatga
     2341 acttaaaagg aatatgtaca aagttgttgc tatagatgaa gagaaaaaac ctattgaagg
     2401 aatagatgtg tatgaatgtt taaaagatgt tccacacaat attgatgttg tagctattat
     2461 tgataaacaa tctaagatgg atgtgatttt agaagaagta gagcttttgg atatacaaaa
     2521 tatgtggttt gaaaaaggaa gctttagtga aatgatgatt aaaaagacta aagatttaaa
     2581 attaaatata gaatataatt taagtttata tgatgagttg actagataaa attatatttc
     2641 ttaataataa aaatataatt agttaataaa acatattatt ttatagtata gattggttaa
     2701 taaaagtaca gattggttaa tgaaacatta aattacaata tttgttaaaa aaattgtgag
     2761 ttacatgata taatgtatta aataggatac taaggagttg atatattatg ttaatagtaa
     2821 ctacagaaaa agtagaaggt aaaaagatat caaaggtttt aggattagtg agaggaagta
     2881 caataagagc aaaacatgtt ggaaaagata taggagcaag ttttaagaat cttgttggag
     2941 gagaacttac tggatataat gaaatgctca ctgaagcaag acaaattgcc ataggtagaa
     3001 tggttgaaga tgcagaagct aaaggtgcaa acgcagtaat agcatttaga ctgtcttcag
     3061 cttcagttat gcaaggagca gcagaaatgc ttgcttatgg aacagcagtt gttttagaag
     3121 atgataatag tattcttgaa aaataaaata taaatagtta aaaagaggca tcttttaaaa
     3181 attttatgga tgcctttttt tatttgaaaa aactaatttg agaagaaatt ttaactatta
     3241 tgacaataaa attagggtga tattatataa ataagta
//
LOCUS       DAMJYX010000197         3264 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJYX010000197 DAMJYX010000000
VERSION     DAMJYX010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.202"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            124..771
                     /locus_tag="PC479_003487"
     CDS             124..771
                     /locus_tag="PC479_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2806000.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(938..1507)
                     /locus_tag="PC479_003488"
     CDS             complement(938..1507)
                     /locus_tag="PC479_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806001.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1512..2411)
                     /locus_tag="PC479_003489"
     CDS             complement(1512..2411)
                     /locus_tag="PC479_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2806002.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2422..2571)
                     /locus_tag="PC479_003490"
     CDS             complement(2422..2571)
                     /locus_tag="PC479_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806003.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2564..3148)
                     /locus_tag="PC479_003491"
     CDS             complement(2564..3148)
                     /locus_tag="PC479_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2806004.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1146 a          555 c          368 g         1195 t
ORIGIN      
        1 actattttcg taatcttagg aaaagattac ttataaaatt aaccttatac aataatatat
       61 cgtataatct taattagcat aatagttaca atattaaaat ttaaatatgg gagtgatttt
      121 agtatgataa gacaaacacc attagagaaa cgatatgaca agctaggtcc tcatattgta
      181 acagcactca aaaaacgtta ttttgatgct tattactgta aaactagaaa tgaagctttg
      241 caacaaattt tagatttaat tcctcaaaat catgtagtat catggggagg atctgaaaca
      301 cttaaagaaa tgggcgtaca aactgctgtt agagagaaag gatttaaagt tattgataga
      361 gatttagcaa aaactccaga agaaagaact gaaattatgc gtcaagcact tctatgcgat
      421 acttttttga tgagtagcaa tgcaattagt gaagatggac aactctttaa tattgatgga
      481 aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa atgttatagt tgtagctggt
      541 atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa gaactagaac aattgcatct
      601 ccagcagttg ttcagcgttt tcaagatgtc aatacaccat gctatattaa tggaagttgt
      661 atagattgca caagtcctga aagcatttgt gcatacatgg ttactacaag aatcagtcgt
      721 ccagcaaaaa aaataaaggt aatattggta ggagaaaatc ttggtctata aaatatacat
      781 acatgtaaat agctctataa ataataaaat catatgaaat aacttatttc taaaagtggt
      841 agaataaaag cttctacaag actttcattc tgccactttt atattctttt aatttgtgtt
      901 tctatattat taattgctcc aattttcata tatattacta actattttta ttttcataat
      961 ttttttgttc ttcatcaagc tgtttataat aatctttatt atagtcagct agataatcta
     1021 taccacctcc tgtaatcaca tttaataatt tcattgcaga ttcttgatta actttacaaa
     1081 cataattatc accagattta acaagctcta atgtaatatt aaattctttt ttctttgcag
     1141 aatcaatatc tttcatagtt ggatttggca tcatttctcc tagtttagga ggattctttt
     1201 ttaaaataga aaccatatca gtattgttaa tttttaaatc tacagtcact ttatccttat
     1261 tattttttat atctgaaatt tcccaagtca tatttttaga atacatttta aatccttctt
     1321 tatcttcgta tataagatta cctaaccctg attttgggaa atacttatca acagtatctg
     1381 catcttgtgt ttttattgaa ttaaacatac tatccacttc attttttatc ttattatcat
     1441 tagtacaacc agctaacacc agtactgata ttgcaaacat tataatacat agaataaatt
     1501 tcttcattaa attatatccc cctatacttt tttgcacaaa tattattctc ttttttatct
     1561 tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt ttatacattc accacaccta
     1621 atacagtctg gactatttac atctttatat attggtatat caagcttaca ttttgtttga
     1681 catgctgtac aatttgtaca tttatcttta tcaattttaa atctatatat tgaaatagga
     1741 ttaaaaaaac catagattgc tcctaaaggg catatatact tgcaaaatgg cctataagta
     1801 atgattgaca tcaataatat caataacaaa atacttaact tccagctaaa taaaaatcct
     1861 attgattctc ttaaattctc atttcctaaa ataagtggta ttcctcccaa aagtgttcct
     1921 gaagggcaaa tccatttaca aaaccaagga tttccacccc ctgctacatt tacaatagca
     1981 actggcaaaa taattacaaa tattattaag actatatact ttagatattt tagatattta
     2041 tcaccataaa ctttttttat ctttactgga aagggtactt tatgtagtaa atcttgtaca
     2101 agtccaaagg gacaaagcca accacaaaca aatctgccaa aaaaagcacc aaatgccatt
     2161 aaaaaaccaa caacataaaa tgagaattta tactctctgc ttgataaaac tgcttgtaat
     2221 gaacctattg gacatgctcc aagtgcacct ggacatgaat aacaattcaa tccaggtaca
     2281 cataattttt tactttctcc tccatatata gttccttcta aaaatccatt tacatatcca
     2341 tttgttaatg ctgtaaatgc aacttgagta ataagtctta tattattatt tactttagat
     2401 aataacttca ttcctcttct cctaaccaat accaatacac tcaagacaaa tcataattgc
     2461 tttagttaat actgtatttg tttctccacg atatgtacct aatgcaataa atataatact
     2521 aattgcaata atagaaatgc cttttttatt atctttaata aagttactca ttggctttta
     2581 ccatttttag tctttcatct ataatttttt tccaatcttc cttactattt gcacctacaa
     2641 ccttctcacc cacaaagttt cctttcttgt ctacaaaata agttgttgga aactccataa
     2701 ctcttttaaa atcagttttt ttaagttctt cagatacagt caattgttgg taagttgcat
     2761 ctgacttttt cataatgtct ttaactaaat ttttctcttc atttgaaaga ccagttctca
     2821 tatcagtttt atcaacttct acaactaatc cctttatagc tacattggaa ttattactct
     2881 catattcttt tgataatgct tcaagctctg gcatttcacc cacacatggt ccacaccaag
     2941 tagtccagac attaataaga gtaagttctt tatttgaaaa tatctctttt gttaccttat
     3001 ttccatcaac atcctccgta tctaaattac taaaccattc actattattc ttgttttttt
     3061 ctactggctt agattctttt ttgctacatc cagaaatcat acctaaaaca caaatacatc
     3121 ctaaaactac tatgaaaaac cttttcatat ctcacactcc tagaatttat atagtatata
     3181 aaatcgctgc ttgaaataaa aataacttat cttctatatt aaagtaatta tatttaataa
     3241 ataagagcta atttaaaaaa agat
//
LOCUS       DAMJYX010000198         3256 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJYX010000198 DAMJYX010000000
VERSION     DAMJYX010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.203"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..403)
                     /locus_tag="PC479_003492"
     CDS             complement(<1..403)
                     /locus_tag="PC479_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2806005.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIR"
     gene            complement(551..1342)
                     /locus_tag="PC479_003493"
     CDS             complement(551..1342)
                     /locus_tag="PC479_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2806006.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(1378..2034)
                     /locus_tag="PC479_003494"
     CDS             complement(1378..2034)
                     /locus_tag="PC479_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806007.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(2027..2992)
                     /locus_tag="PC479_003495"
     CDS             complement(2027..2992)
                     /locus_tag="PC479_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2806008.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(3098..3204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1002 a          582 c          342 g         1330 t
ORIGIN      
        1 ttcttataaa gtaatctaat atcacaaaag cactataaaa aattagtatt gggcttattc
       61 ttagtttttt tactttagaa atggttaagt atataaaagt agacatatta attatgctta
      121 agttaagcaa tacaatttgg aatttttgaa tttttaaatt tactactcct aaaatactta
      181 caaaaactaa tacaagacac cctactttca atatatttat aaattcatct gttttaaagc
      241 tacttatata aactatagaa tatccaaata tccaaagtaa aattattcta tacatattta
      301 tagaaactgt atacaaattt atatttaaaa gtgcaataac caaaaataac ccattagcta
      361 gtatatagac cttattctta cttaaagttc ctttagattc catattcccc accacttctt
      421 catttttata tagttttacc tataaatatg ttaccacaat tcttccaaaa aatttgtcac
      481 tttctagtta taaaaaaaaa tgagtaaata tttttaattt tactcatttt gtagattttg
      541 tatattcagt ttaaaaagct ggaactatac ttcctttata ttcatcttgt ataaattttt
      601 taacttcttt actattcata gcctctgata aagccttaat cttatcacta tctttattat
      661 tttctctaca agctattata tttacatatg gagaatcact tgattctatt actattgcat
      721 ccttagtagg atttaagtta gctgttaaag cataattaga atttattact gctccgtcaa
      781 catcttttaa tactgttggt aattgctctg cattcatttc ttttattact atatttttag
      841 gatttttagt tatatctttt ttagttataa gctctccatc ttttacttct atcaatttat
      901 tttttgctaa taatttaagt gctctagctc cattagttgc atcatttggc acagctataa
      961 cagctccatc ttttaattca tctaatgact taactttttc tgaatagaat cccattggct
     1021 ctatatgaac tttagatgta taagtaagtt tatatccttt ctccttcaca gtttcctcta
     1081 aaaatggaat atgttgaaag aagttagcat ccaaagaccc ttcatctaaa gctgtatttg
     1141 gaagtacata atcatcaaat atttttactt ctaaatcata tcctttttct tttaacaaag
     1201 gttttgcaac ttccaatatc tttgcatgtg gatttgatga agctcctact agtatttttt
     1261 tatcctcttt gtttgaacaa cctactgctg atattgctat tgctgacacc aatgctactg
     1321 ataataattt ctttaatttc atttttactc cccctcttta aaatgtttat aattaattta
     1381 ttttagtttc ttataagcca gatttcctac tccctggatt atctgaacta atattataag
     1441 tgctattaca gtatatacca ttactgctgt atcaaatctt tgatatccat aaataagtgc
     1501 tatattacct aatcctccag ctcctactgc acctgccata gctgtataac caagtataga
     1561 tattattgat aatgtaattc cagatactat tgaaggcata gcttccttta tcataacttt
     1621 aaatactatt tgtctttttg tagctccaaa agattttgct gcttctatca atcctttatc
     1681 aacttcattt aatgagcttt ctataattct agcgataaac ggtgctgccc caatagttat
     1741 tggcactatt gcagcagttt cccctatact tgttccaacg attaatctag ttattggaat
     1801 cattgcaact ataagtatca taaatgggaa acttctaaat atatttacta taaatcctaa
     1861 tgcactattt attgtgctat ttggttttaa tccatctggt tttgtaacta ctaatatgat
     1921 tgctaatata aatcctaata ttgttgccac tatagttggt actataacca tatacaaagt
     1981 ttgtaacaat gcatttggaa atagtgttgt taaaaagtca attaaactat tcatttatca
     2041 tttcctccca tcttatgccc ttactagtta gatattgttt tacttgctct ccacttttat
     2101 ctgaaatgtt tataataagt gaacctaaaa tatcatcttt aaattgttct aatttcccaa
     2161 atattataga cacatctata tttagttctc ttgccatagt cgtaataata gcttcattag
     2221 aaatatcttt agggaatagt atctttatat ttgttccttt tggcaatatt atactttctt
     2281 cacctattaa ttttctaagt cctttagtat ttcttaagaa tatttcttct gtatctccta
     2341 cctctaatac ttctccattt tccattattg ctactcttcc acaaatctgc tttataactt
     2401 ccatttgatg agttacaact attattgtta ttcccaattt tttatttata tcttcaagca
     2461 atgaaagaat tgactttgta gtatttgggt ctaatgctga agtcgcttca tcacaaagaa
     2521 gtacttgtgg atttagtgct aaagctctag caattgcaac cctttgtttt tgtccgccac
     2581 ttagatttct gggtttatca tttttctttt cagatattcc aactacttct aataactcta
     2641 aaactctttt tttaatttct gcctttgagt atccaaaaca ttctaatggc aatgctacat
     2701 tatcaaatac agtttttctt tcaagcaatg aaaagtgttg aaaaatcatt cctaactctt
     2761 ttcttaaatc tctcatttgc ttttcattca atgactttac ttctttatca gaaacaagta
     2821 cactgccttc ttggtattct tctaatccgt ttatacatct cagtagtgtt gatttccctg
     2881 caccactatg acctattatt ccaaaaattt ctccagattc tatttcaata cttacatctt
     2941 taagcacttg aatctttcca taatacttat taacattttt tatgctaatc ataaaatttc
     3001 ctcccatata ttctgctcag tttttatgtt tatcctctct tatgtattac aacaaaataa
     3061 aagcccgagt ataaactcag gcttagaaag ctatttatac tcatctatca actatattag
     3121 ttgcaggatt tagcaccaat gtatataaaa tatactggtt gccgggtttc aaagggccag
     3181 tccctccacc actcttgata agacgttatt taatttaatt ctattatcat ttgttgctat
     3241 gttatatatg atatat
//
LOCUS       DAMJYX010000199         3000 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJYX010000199 DAMJYX010000000
VERSION     DAMJYX010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.204"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(92..1039)
                     /locus_tag="PC479_003496"
     CDS             complement(92..1039)
                     /locus_tag="PC479_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806009.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(1067..1558)
                     /locus_tag="PC479_003497"
     CDS             complement(1067..1558)
                     /locus_tag="PC479_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806010.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(2001..2894)
                     /locus_tag="PC479_003498"
     CDS             complement(2001..2894)
                     /locus_tag="PC479_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2806011.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         1001 a          423 c          264 g         1312 t
ORIGIN      
        1 taatcattta tatatatgat aaatatatca atatctattt tcctttaacc aaatgctatt
       61 ttaataagtt taagttttag ctttaaaata cttaataata caataaaagt gatgtattta
      121 caaataattc ttctggattt aattcattta ttgatgtatg ttcaattttt atatttatat
      181 ttttcatatc ttcataaata attccaactg gagaacaaat gaaacaagaa aatccaaaag
      241 tttttgttat tttttttacc ttattatcac caaaacttat ttgcttttta caatcgttta
      301 tattttcatt gttattttgt atatcatttt tataatactt atctttttta atgtcattag
      361 acttatttac tgttttaaaa ttactcctat ttttacaact atagtctatt tcaacttcta
      421 tatacccaaa aactatcaag ttacataatt tcaaattact acctttgttt ataagatttt
      481 tcataggttt aacatcaaat attttactat ttacatttac ttttaatata tctcttagat
      541 ttttaggagc ttttaaagta ttacttatat tcaattgttt gaatacaatt ttatttacat
      601 tagtataaac tctattactt tctattgata ttcttgttgg gaatttatct agattacact
      661 tataataata gtatatatta gcactattct ttataatcct atgtgcagaa tttggtaaaa
      721 ctacactctt aaaagatatt atttttttac aacttgcatc tattctccct atgtaaaaac
      781 cttgctgtgg gtctaatcca cgttttataa tgccattaac ttttaccgta ttatttataa
      841 actctatata tttagaaata taatctttga aaaatatatt tccagttttg tacctacttg
      901 tatttgtaac aataatagtg taggtcaaaa tctcaaatac agatgcacat tttttatcaa
      961 catatttttc tacttttata atacttccat ttgcatcatt tgaaaataca tcattaaaag
     1021 tagttttcgt tattctcatt gcatccacct ttctataatt taaagattac tctatcttgg
     1081 aactattttc ataacttata attgcagtaa cactaataaa taaattatca tctaaaatag
     1141 atgaagtaat atcttgtata agatacttta aattaatttt atctttattt tctatctttc
     1201 taggcatttg tataaatgta ctaaagggta tttttaattt aaatgtattt tttttactag
     1261 aatttctatt tcttttattg cctgcataat ctaatattgc atcaatatct atatcaccaa
     1321 ctaaaatcaa cttctttcct gtaagttgtt catcttctaa tgatatacat tctatagtat
     1381 caacaagact tatattgtta atattcatat tgtaaacata tgtctctaat gaatttatta
     1441 cagttttttt agaagctaat ttaaaatttt tatatgtatt aaattgagat aaataaagag
     1501 tcttttctag tttttcacat ctttcaaggt tgtatattac cttattattt tcatacataa
     1561 tcgcatcaat ctccctgtat ctatatatta atactattca taaaaattat ttcaaattac
     1621 cattttttat tattcactat ctagttgtta tttactttta agaaagaatc cttttttact
     1681 tcattgattt caatttacaa gtaaaagtct ttctgaaaaa aaaatatata tatctctatt
     1741 cactttttta taagtaatta taatttttat agactaacgg tcagccttaa gggaaaaggt
     1801 gttctttaag tgcagaattt agataaagta aataatcctt ttataaataa ttccaagttt
     1861 taacttaaat aatttgattt acagtataaa gtaaattttg aaagataatg atatcgattt
     1921 aataattaca agtaaaagca tgtcaaaata aggctatgat tttaaaaatc atagccttat
     1981 caataaaaat ttaatcaaag ttacatttca ctttgaattt cactgtctat taattgaaga
     2041 ttatactgtt gaattaattc tattagacga gctgcatata tactattgcc attttcatcc
     2101 ttcgcagtac tataacctgc cttttctaat gctttagctt gatatatata cgtttttgca
     2161 tcaaatacac cattgttttt atacctttta tttgtggcta aaaattttgc atggtctttt
     2221 atagattcat ttttatcatc atatattcta aatttatcat ctataattgt atctaaatgt
     2281 tctttcgttt ctaaagtcac tgacttgcct ttccaataag cgtcagcctt tataccaaat
     2341 agattgttat attgttttgc caaagttgac ttgccccagc tagattctat tatagcttgt
     2401 gcaatagtta tagatggaag tattttataa tccttataat tttgtattgc ttctgtttta
     2461 atttcatcaa taaacttcat atattttgta tcagacttca atctttctgg ttttatacca
     2521 taatccttaa gattgtctat ataattatca accaatcttt tttgtctatt actaaattct
     2581 aactcatcca aaatatcact taacttattt attttattat ttttagaaaa attcttaaca
     2641 aataaatctg atacttcttg tatctgtgaa tccttaacgt tttttaagtt atttttattt
     2701 aacacagcaa caatggaagc tacatacttc cagttaactt gtgcattatt tttacttgct
     2761 aaatcagcat attttatata cttactgaca tttatatcat ccttttttat ctctctagat
     2821 gaatattcct tcactaatac acctattaaa attgatatta tcagtattat aacaatgaat
     2881 tttttattct tcataaatat tgtcctccct tatactatct gctgaatata atatacctta
     2941 cttttgtgac atattcttta cgaaattttg aaaattttat gaagatttat gaaatatatt
//
LOCUS       DAMJYX010000200         2996 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJYX010000200 DAMJYX010000000
VERSION     DAMJYX010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.205"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            1192..1581
                     /locus_tag="PC479_003499"
     CDS             1192..1581
                     /locus_tag="PC479_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2806012.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2451..2921
                     /locus_tag="PC479_003500"
     CDS             2451..2921
                     /locus_tag="PC479_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2806013.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT         1352 a          170 c          545 g          929 t
ORIGIN      
        1 gaaaatttat aatataatta atgaaaaaat ttaaactttg taatatataa ataaaaaaac
       61 tctaaaatag aaggatataa ttatatagaa tgtttcaatt aaacaaggta aaaactataa
      121 tcttaaaatt aaaattaaca ttattcttaa gaagcaagaa atttagaaag taggaagttt
      181 agtgataagt ggtagtaagt aagaaattta ggaagtggga agtttagtta taagtggtag
      241 taagtaagaa atttaggaag tgggaagttt agttataagt ggtagtaagt aagaaattta
      301 ggaagtggga agtttagtta taagtagtag taagtaagaa atttagtaag tgggaagttt
      361 agtagtaata atttatagca agtaagaaat ttaggaagta ggaagtttag taataatttg
      421 tagcaagtaa gaaacttagg gagtaggaag tttagtaata atttatagca agtaagaaat
      481 ttagggagta ggaagtttag taataatttg tagtaagtaa gaaatttaga aagtaggaaa
      541 tttagtaata atttatagta agtaagaaaa ttagggagta ggaagtttag taataattta
      601 tagtaagtaa gaaatttaga aagtaggaag tttagtaata atttatagca agtaagaaat
      661 ttagggagag gaagtttagt aataatttgt agcaagtaag aaatttagaa agtaggaagt
      721 ttagtagtaa tttatagtaa gtaagaaatt tagtaagtga gaagtttagt tataatttat
      781 agtaagtaag aaatttagta agtgagaagt tcagtaataa gttgtagtaa ataaaaagtt
      841 taggaagtag gaagttcagt aataagttgt agtaaataaa aaatttagga agtaggattt
      901 actgaaatac aaatatttaa aatcaaagag tttaaaagaa aggagagcta ctggacaagt
      961 atccaaaata gatagctgag tacaaaatat ttcataaaat taagcaatat aatacaagag
     1021 aattttgaca ttatttatat atatttaccg tgtacaaata gctaaattta ggattaagat
     1081 tgaaaaatat cttataagtc tgagcttata taaatccgta taagatgtat ttaatcgaaa
     1141 aattttatta tggatattgc ttgtctatat tctgagtagc aaggttttat catgactata
     1201 aaaaagttgc cacaatcaga attaaagata atgaaattta tatggaaatc agattccaaa
     1261 gtaacatcta gagatattgt tttaggaatg gagcaaaaat atcaatggaa acaaacgaca
     1321 actttgactc ttttatctag attagtagta aaaagatttc taaattctca aaagatagat
     1381 aaatatacgc attatgaagt tttaattaaa gaaaaggaat acataggagt tgagacaaga
     1441 gattttttta gaaatattca tgattcttct ataaaaagtt tattgttgtc attacatgaa
     1501 aatattaatt tgagcaaaga tgatatttta cttattgaag agtggataaa aaatctaaaa
     1561 gaagaggaga aagacgtata agcaaacgta tttcttatag catcaatata aagtgtatgt
     1621 aagtgtattt atgtaatata ttagtctact taagtaatat gacaaatgat tataagtgga
     1681 ttatgatact ataatgtgaa tctaaataaa ttaaatataa attaatagtg gatattctat
     1741 ttaattaaat ttaaaaagct acctttactt ataagtataa ttaaggtagc tttttaaata
     1801 gaggtagtgt aattaaagag aatttttaaa tataaattgt gaagctttaa tatagatgat
     1861 aatatatatt ttgcaaatat gacacagtgt tttcgtattt aagatgataa aataaatagg
     1921 aattaatgat gatattgaaa aaataaaaat aaaggattaa tataagatat taaagagata
     1981 gagataaaag tgttaacaga agatattgaa aaaataaaag attgatataa gatactgaag
     2041 aggtagaaac aaaagtatta atagaagata ttgaaaaaat aaaaacaaaa gattgataga
     2101 aagtactgaa tagatagaga taaaagtgtt aatagaagat attgaaaaaa taaaagattg
     2161 atataagata ctgaagaggt agaaacaaaa gtgttaatag aagatattga aaacataaaa
     2221 acaaaagatt gatagaaagt actgaataga tagagataaa agtgttaata gaagatattg
     2281 aaaaaataaa agattgatat aagatactga ataggtagaa acaaaagtgt taatagaaga
     2341 tgctaaaaaa gtaaaaataa aggattaata aaaggtactg aagagataga gaaaaaaatg
     2401 ttaatagaag atactgaaaa aatagaaata aaaaattagg aggaaaattt atgaaatatc
     2461 aggtttcatt attagcagtg aaggatattg aagtatcaaa aaaattttat aaagaattat
     2521 ttgagcaaga aattgaattg gacttaggca aaaatgtaac atttaaagga ggatttgcta
     2581 tacaagaaga cttcgcatgg cttacaggga ttaatccaga tacaattata caaaaatcaa
     2641 ataatatgga actttatttt gaggttgatg attttgacga atttattgaa aaactcaatt
     2701 attataaaga tgtagagttt gtccataagc cactaaaaca tgaatggcaa caaagagtag
     2761 tgcgtattta tgacccggat aagcatatta ttgagatagg agagtctatg gaagttatag
     2821 ctagaagata tttatctgag ggatactctg tagaagaaac ttctaaaata attcaacatc
     2881 caattaaatt tgtaaagcag tgtgaaaata gtaaggaatg attgtttaaa ttataattat
     2941 ttagtagaaa ttaattgtaa tagaactaat tcaagataat atataggact agtatt
//
LOCUS       DAMJYX010000201         2978 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJYX010000201 DAMJYX010000000
VERSION     DAMJYX010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.206"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(4..612)
                     /locus_tag="PC479_003501"
     CDS             complement(4..612)
                     /locus_tag="PC479_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2806014.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(721..>2978)
                     /locus_tag="PC479_003502"
     CDS             complement(721..>2978)
                     /locus_tag="PC479_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2806015.1"
                     /translation="LNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLI
                     GVIYLLLCNHTYYVYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQ
                     RTLKNYIDQRKELLRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTF
                     IKEDEKVAEKLKKFDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKI
                     DEEIRNINKDYENIILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMT
                     LEQYFETFNIDFFDNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEV
                     RVGDYWSDNKLFKLLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRV
                     LYTNRAFRELFGISSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEV
                     KMISRTLYFGQKRSRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKT
                     PLNNIYSSVQLIENLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSD
                     IYKIKAVNFDIIDITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNI
                     LSNAIKFTLEGGEILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISN
                     EFGSGIGLCLTKSLIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIE
                     KFEIEFFDIYK"
BASE COUNT          936 a          486 c          241 g         1315 t
ORIGIN      
        1 aacttattct atataaaaac gccataaaaa atcttttgct tcttctgcat aatctatatt
       61 tatacgttta ctagttttta tatctaactc atctttacta aatacttttt ttcctttatc
      121 atcataataa aaatcactta tatataattc ttcacccatt atactcttac tatttagact
      181 tctatctatt tttaaagctt tgcaaagttt tcctggtccg ttagtaatat tttttacttg
      241 atatttactc aactcagtgt aagctttgtt gtatctattc atagatatct catctaaaga
      301 agttataggc tctacccctc ttattaatac acactctggc acattttcta tatttgcaga
      361 taaattcaag caattataca taccataaat taaataaaca tatatatgtc caccatctaa
      421 atataatggc ttagttctat ctgtcttttt attgccatat acatgtgctc ctttatcatt
      481 tacacccatg tatgcttctg tctctatgat tttggtaaca attactttat tttcatattt
      541 ccttattaga tatttaccta atattgattt ggctaaatca attccatttt ttctaaaaaa
      601 atccttttcc aaaatttcac ctcataaaat ttatttaaaa cgcatcaaat acaactaaag
      661 taataaataa aattaactct tattaatgtg atatatataa acaataaatt tagacattta
      721 ttatttatat atatcaaaaa attctatctc aaacttttca atattatcat tgtaatttga
      781 attatcatat acttcttcaa caacctcttt aattgggaat ataactttta cattagttcc
      841 ttctcctacc ttactatcta tgtaaatctt cccattttga atttcaatca atgattttgt
      901 aaggcataaa cctataccac ttccaaattc atttgatata ccactgtttt caatttgttt
      961 aaatcgatta aatatatcct ttaatttttc tttgtctatg cccactccag tatcttttat
     1021 aattatctct actgtttcgt cttttttata tatacctaca aggatttctc ctccttctaa
     1081 agtaaatttt atagcatttg ataatatatt tagtattatt ctttctatcg attctgggtc
     1141 aagccctacc ataacagttt cttcatctgt atcaaaaatt aaatctattc ctttagactt
     1201 agcataacta ctaatagatg taactattct ttctgttatg tcaataatat caaaattaac
     1261 tgctttaatc ttatatatgt ctgacttaac ttgagaaata tttattatat tatctattaa
     1321 tctcaataat ctaaacatat tttgtttagt tatttttata tgttctttta gtatatcctg
     1381 aaaatcaatt atttttcctt ttttataaag gttttcaata agttgaactg aactatatat
     1441 attatttaaa ggtgtcttaa gttcatgaga tatattagca taaaactcca tcttcatttg
     1501 gtcatactgt ctttgtttta ttaatgcttt tttaagtctt tcagactctc tttgcacact
     1561 taaatcttta ataattctca ctctacttct tttctgtcca aagtataatg ttcttgatat
     1621 catttttact tctttaatgt gattattttc atttactata tggatactct tctctctttc
     1681 tcctatattt aaattgttta tgtatttttt tactaatata tttctatatt tttgtgttgt
     1741 aactcctaat acatcactag aaattccgaa caactctcta aaggctctat ttgtatatag
     1801 tactcttgat gtacttaaat cctcaagaaa aatagcctct ggtataacat ccagtaattt
     1861 ttcatatatt attttttgtt catttactgt tctaagaata tttatctctt tagttatatc
     1921 tctaaatgta agcaatttaa agagtttatt atctgaccaa tagtctccaa ctctaacttc
     1981 ttcaacatgt tcttcacctt ctgagtcaga catcacaaaa ttaactatac agtttgctgt
     2041 gtcattgtaa cttactattt cagctttctt ttcttgtgtt agatttaatc ttttaaattc
     2101 cctattgtca aagaaatcta tgttaaaagt ttcaaaatat tgttctaatg tcatatcgac
     2161 taaatcactt gaaacacatt taaaactctt ttcatatttt ttatttatac ttattacctt
     2221 attcttcttt atgccacctt cagcacttct tacaagtata ggagatggaa tatttagtat
     2281 aatgttttca tagtctttat ttatatttct tatttcttca tctattttga tttcatttgt
     2341 aatatcattt aaacttatta agtaattgct ttcgtcttta ttattactta aacttaacaa
     2401 ataaatttta tcatttaact ctaccagttt atcaattata cttatatcgt tatcaaattt
     2461 cttcaatttt tcagcaactt tttcatcttc ctttataaaa gttaatattt tataataatt
     2521 attcatatcc tttgaaaatt catctttaaa tttattattt gtgtaactta tattaaaatc
     2581 atagtctgtt actatccata catttggtat tgtatccaga gcttgtctaa gtaattcttt
     2641 tctctggtct atgtagtttt ttaatgttct ttgtataatc atatcctttt ctattgctaa
     2701 atttatcttt cctaattgct ctgacttttt acttaatttt ttattgattg aatcaagttc
     2761 ataatatgga tacactactc tattattaag actgtataca tatacataat aagtatggtt
     2821 acataatagt aaatatatta ctcctattaa tctatctgaa tctactatat taatcttaga
     2881 aattgctaca aaacatataa tagttgtaaa aaaaacaaaa tatgttaaat cttttattaa
     2941 cttatagtgc tcaatgttac tatcaagttt attcaaca
//
LOCUS       DAMJYX010000202         2911 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJYX010000202 DAMJYX010000000
VERSION     DAMJYX010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.207"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            51..350
                     /locus_tag="PC479_003503"
     CDS             51..350
                     /locus_tag="PC479_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2806016.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(589..2817)
                     /locus_tag="PC479_003504"
     CDS             complement(589..2817)
                     /locus_tag="PC479_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2806017.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT         1036 a          395 c          396 g         1084 t
ORIGIN      
        1 agaataaagg gtataggtaa tatataaata aaatggagga attttagaca atgtataaga
       61 atataaataa aaagcaactt aaagaaatga tgaaaaatga aaaggatgtt ttattattag
      121 atgtaagaac taaagatgaa tttaaggaat ataaaataga aaatgctata aatatatctt
      181 tacaagagtt aatcaataat atagatgaaa tttacgatta taaagataag aaagtagtag
      241 tttattgcag aagtggtcat agaagtgtta ctgcatgtaa tttgttagct gaagaaggtt
      301 ttgagcattt atataattta aattcaggaa taattgatta tatgagttaa ataatgtaag
      361 ttatacatta aatactatgt atagctctgc ttattaagta ggaatatatc taaatattta
      421 taagataaat ttaggaaaga acgaattttt tcgttttttt ctatgacagt ttttgtagta
      481 aatattagtt tttaagttgg aaaaaggtag tagcttttta gctactacct tttaatatga
      541 acttagaata ttattaaaaa aaatatattt aaagacagaa ttaatttttt aatgaccagt
      601 taaattacta tcgccgaatt tatcattaaa tggttttata actctattta taggtccttt
      661 tagaagtaca tttaaacaaa tagacagaat cataaatagt aatagaacta gtatatcttt
      721 agttagattt ggagcatata taccaccaac tgcttccctt gtagcaccta ttgcataggt
      781 aaatggcatt atagggttta ttctttggaa gaatgtaggt gtaacctgta tagggaatgt
      841 accaccagaa gaaccaatct gaacgactaa tagtactatt gcaattgctt ttcctacatt
      901 tccaaataca gaaactaaag aatacactat aaagttaaat accatactag taaacataag
      961 cagaactatc aataaaattg gatgttttgc tgttactcct aacaataata tatctccagt
     1021 tcctattata aaaccttgga caagagctat acttaaaaaa gtaaggcctc ttccaaagta
     1081 aatttcataa ggtttatagt ttccatgtac agatgtagac aatagtgctg aaataagtaa
     1141 tataccaacc catgtagaaa gaacactata aaatggtgtc attgcagaac cataattttc
     1201 aattggatat aatttctctg ttttcacctc aacaggttct tttaagaaat tagactttgt
     1261 caatatatca ctttcaagca agctgactaa ttttttcata tcttcaccat tactaatttt
     1321 gcttaaagta tcaatcaaat catccaacat tccttttgct aaaggaagtc tttctcttat
     1381 caatgatata ctttcctgtg catttcctga aagttttaga gaagtagtaa gtatttcttc
     1441 aacctttgga agttttgctt ctgccttatc taaaacagta attatatcat ttgctacttt
     1501 tatactgttt gcaaatatac tatttatagg ttttgatatt tttgagtcaa aattatttaa
     1561 aatatttaaa ttaattcttc caataccatt tgatagactt aaaacattat tcaaagcact
     1621 tatagatggt tgttgtccag atataacttt gtttttaata tcatttaatg ttgatataga
     1681 tgaatctatt ttattgctag agtcttcaag attatctatt acatcatcca atctattatt
     1741 tgatgtaagt tggtttaatt tagttaaaaa gtccaccaaa gtatcgttta aagattgcaa
     1801 attagaaagt ttttctgata aattatctat caacttaggt acatcttcac tgccactatt
     1861 tactgcatca attaaattta atgtaagaga agaagcagaa gatgaaaggt caaccattaa
     1921 atttaagtca gacttaataa gaggagaaag ttcgtctaaa ttatcatttg tatcctctaa
     1981 gaattttttt aagtcacttg ataatagttt tgtatcattc agagtctttt taattgtagg
     2041 taaatcagat ttaatatctt taactgtatc agataatttt gaagtagcat cagaagctaa
     2101 atcaacagtc ttatttatat ggtcaaatct cttttgaagg tcaataaggg aattttcaac
     2161 ttttgtcaat tttggaagac tgtgttcaat tcctgcacca actccgtttg atataccaag
     2221 aacgatttca ctaactgttt ttactatagt ctgattaacc tgtagttgta ttgtagaagc
     2281 acctttatct gtaatctttg gtgcaactgc atttattttt tcattaacag tatagataat
     2341 ttcgcctttt ttaacatcat ctgtaattaa agatgtcaaa tcttttgtaa aattttttgg
     2401 aatcttaagt gatgcataat aagtaccttt tttaacacct tcaagagcag ttttttcatc
     2461 tacaaactgc caacctaaac tcttgttatc tttgagttgt tcaattaatt ggtctcctac
     2521 attaatcttt tgtcctgcta tttcatttcc tttatcttca tttgttactg ctacaagcat
     2581 attagcagtg cttccataag ggtcccaaga tgcctttata ttgaaccaag catacagaga
     2641 tggtaaaaaa gcaagaccta aaactattac aagtacggca taattgccac gtatatcttt
     2701 taggtcactt ttaaaaattt ctaatatatt ttttaaaccc tttgaaggtc ttaaattctt
     2761 gatattattc cagtttaaaa tttcttttaa actattagtt tttttcaaat gtctcacctc
     2821 ctaaaatatt ttgataaaga aaaaagttag atttataatc aattttaaat tatattactt
     2881 tttacacaaa tagtaaagtt aaaattttgt c
//
LOCUS       DAMJYX010000203         2856 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJYX010000203 DAMJYX010000000
VERSION     DAMJYX010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.208"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..1340
                     /locus_tag="PC479_003505"
     CDS             <1..1340
                     /locus_tag="PC479_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2806018.1"
                     /translation="RYVIPIKSSYKNEVNGTILDTSSKGNTVFIEPISVSNLSTELTM
                     LKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVFAKAKYSQKIKGITPKIN
                     NNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAGGKTVTLKTVGLLTLMVQ
                     CGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIKNIAEIMSLSNNSTLVLF
                     DEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKKFATLHPEFENAGMMFDK
                     ETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVIDRNYNLDLVKRSKLQSN
                     IEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFNNVEVLFNDNFISVNIKR
                     LQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKALKKIQKEIRENRK"
     gene            complement(1403..2026)
                     /gene="ytaF"
                     /locus_tag="PC479_003506"
     CDS             complement(1403..2026)
                     /gene="ytaF"
                     /locus_tag="PC479_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2806019.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            2214..>2856
                     /locus_tag="PC479_003507"
     CDS             2214..>2856
                     /locus_tag="PC479_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2806020.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         1209 a          308 c          422 g          917 t
ORIGIN      
        1 atagatatgt aataccaatt aaatcttctt ataaaaatga agttaatggt actatattag
       61 atacatcatc aaaaggaaat actgtattta ttgaacctat tagtgtttca aatttaagta
      121 cagagttaac tatgctaaaa gctgatgaaa ctattgaaga atacaaaata ttatcttatt
      181 taacagaact tatatttgag aagataagtc agataaaatt aaatatagag atactttcag
      241 aatacgatat ggtatttgca aaagctaagt atagtcaaaa aataaaagga attactccaa
      301 aaataaataa taatggctat ataaaaataa ttaaaggtaa acatccactt ctaacaggag
      361 atgcagtacc tttagatttt gaaataggca aaaattatag aagtcttata ataacaggtc
      421 ctaatgcagg aggaaaaact gtaaccctta agactgttgg tttactaact ttaatggttc
      481 aatgtggatt tgatatttct gcaaaagaag gtagtgaatt tagtgtattt gaaaaagttt
      541 ttgtagatat aggggataat caaagtattg aaaatgcctt aagtactttt tcatcacata
      601 taaaaaatat tgcagagatt atgagtttat caaataattc tactcttgtt ctgtttgatg
      661 aaataggaag tggaactgaa cctaatgaag gagctggttt agcaatatca ttattagaag
      721 agttttacaa aatgggatgt ataactatag catcaactca ttatggagag ataaaaaagt
      781 ttgctacatt gcatccagaa tttgaaaatg ctggtatgat gtttgataaa gaaacgttag
      841 aaccattgta taagttaact attggaaaat ctgaagatag taatgctctt tttatttcta
      901 aaaaaatggg aataaaaaat agagttttag atagagctaa agaatatgta attgatagaa
      961 actacaattt agatttagtt aaaagaagta agctacaatc taatattgaa gataaaaata
     1021 gttctgaagt cattttaaac tatacagatt ataatgtagg ggataaggta aaattactag
     1081 atgaagatga ttttggaatt gtatataaac ctatggacaa gtttaataat gtagaagtat
     1141 tgtttaatga taattttatt tcagtaaata taaaaagatt acaacttagc ataaaagcat
     1201 cagaattgta tccagaaggg tatgatttag atgtattatt tactagtttt aaagaaagaa
     1261 aattagaaaa agatattcaa agaggttcaa aaaaagctct taaaaagatt cagaaagaaa
     1321 ttagagagaa tagaaaataa atataaaaag cacaaaagaa agcaatctaa agagaaaagt
     1381 ttatctttag attgctttct tattaacata tcttcataaa tccaatgact aacaagatta
     1441 aaccacttaa ccaagataag tttaaattta cgtttttaac aaacatattt cctagtaaac
     1501 aaccaagtaa tactgcaatt atatttgata ttaaagaaaa tagaataact tgtatataat
     1561 tcacacctac aaggccacta ccaaaaccga tagctaatcc atctaaactt aaagcaagtg
     1621 caagtgtaaa tgcttcagca gatttaagca ctttagattt acttgtatct aagacatcag
     1681 gttcgatact tacattaaaa tttaatttaa ggtctaacat attaaaatta taattctttg
     1741 agtgtataga ttttttctta aaataagatt taaaaaaact ttcaattagt ctagcacttc
     1801 ctaataaaaa taaaataaag aaacatatta acattgttat attttgaggt aaaaagtctc
     1861 taactattgt acctgcaaaa agcgatattg ctaaaatagc tgaacaaact acgctgataa
     1921 ttatatttga atataaggga actttaactt tatttacgcc atatccaaag cttgtcacaa
     1981 aggcatctat acaaagagaa gtaactaata aaattgattc aatcacagta gattaacatc
     2041 ctttctctta aattttgtac ttatattagt tatagtattg taaaaatgga taaattgtta
     2101 cagataatcc aataaattgt tgataaaatt ccaacatcat acaacaccta aataacaaaa
     2161 gaatcatatc atataataat ataaaaccaa tcataataaa gcgggtgaaa ataatggaac
     2221 aaaataataa ggttgggaat ttcggtgatt tgttgaagat tttattagat gaaaaagaat
     2281 tatcaatggg agaactaagt aaaaaaagtg gaattgataa atctactata tctcgtatag
     2341 ctaataataa acaaaagcca aatataaatc atttagaaaa gatggcaata catttaaata
     2401 ttaacctaga agaactgttg aaagcttcag gatatgaatt tagaaacagc aataatcaaa
     2461 tatttaatgc agatagtgat tttagtaact ttgatgatat tcttggattt gcaaatttaa
     2521 tcaatgataa gaactttaat ggaaatattg aaaaagagtt aagtaagtgt aagttatatg
     2581 tccaaacaga tgaaggtaaa aaattacttt ttgataattt taataaaaaa atagattcta
     2641 ttgaaaaaca aggtcaattt acagatagat taagacaaat gtatgctgat ttttgtacag
     2701 atggacttac aataaagaaa tatttattga ttggaagtat gctactttat tttgttatat
     2761 caacagatgt gattccagac ttcgtattcc caataggatt tatggatgat ttagtagcat
     2821 taaatatagt tacaaaatta cttaaaaatg ataagt
//
LOCUS       DAMJYX010000204         2832 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJYX010000204 DAMJYX010000000
VERSION     DAMJYX010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.209"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            231..1154
                     /locus_tag="PC479_003508"
     CDS             231..1154
                     /locus_tag="PC479_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2806021.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1129..1956
                     /gene="panB"
                     /locus_tag="PC479_003509"
     CDS             1129..1956
                     /gene="panB"
                     /locus_tag="PC479_003509"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2806022.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1975..2823
                     /locus_tag="PC479_003510"
     CDS             1975..2823
                     /locus_tag="PC479_003510"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2806023.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1124 a          337 c          460 g          911 t
ORIGIN      
        1 ttataaatat cataattttt ttactgatat tttattatat aaattttata gaattaattg
       61 tcaatttttt atgaaaatgg taagatattt atggatggta tcttgttata agaactatac
      121 tttaaatgtt tttaattatt ttgtcttatg taactcatat cctcaggaat gagattagaa
      181 attaagatgt tgtatcacaa agtggaggtg atactattaa aataggcttt attggttctg
      241 gaaaagttgg aacatctttg gcaaattatt ttttatcaaa aggttattgt gtttcaggct
      301 tttatggaag gaatcaatta tctttattag aatcaacaaa tttaacaaaa acaaagattt
      361 acagcaattt gaaagatata atttatgaaa acgatatatt atttattaca acatcagatg
      421 attctattga aattatagac aaaaaacttt ccaaatttaa tttaaaacat aaatacattt
      481 gccacacaag tggctcttta aaatctagta tattgttctg ttcaaaaaaa gcaggtgcat
      541 taatttattc tatccatcct atatttgcat tttccaataa aaatactgac ataaaaaaaa
      601 tgaaagacat ttgtttttcc atagaaggtg ataatgaaca agatgattta gttattcaaa
      661 atttcataga tgatttggga aaccaatttt ttataagaga taaggatact tcatccactt
      721 atcatttagc taatgtttta gtttcaaatt tagtattatc tttactagat ataggagtaa
      781 gttactttat taaattaggt ttatctgaag aggaatcttt aaaagctatc agtcctttag
      841 taaaaaaaaa catagaaaat atacttgata atggctttac taaagctctt actggtcctg
      901 tttctcgtgg agatataaca ccaataagga aacatctatc tgtattggat aaaaaagatg
      961 aggatattta caaaatacta tccttaaacc tactaaaaat aattgcctta ggaaatgaca
     1021 attcattaaa aggtataaaa aatattacta tagaaaatgc aatcgaaaat ttaataagta
     1081 aatctgaaaa atacaaagaa atttacaaac ttttaggagg aaaaaacgat gaaaaatacc
     1141 atacagactt ttaagaatgc taaatatgaa ggaaaaaaac tatctatgct tactgcttat
     1201 gactactcaa ttgcaaaaat aatggatgag tgtgatataa atggtatttt aataggagat
     1261 tcattaggaa tggtcataaa aggtgaagaa aacactctat ctgttaccat agacgaaatt
     1321 atatatcata caaaagctgt aaaaaatggc gtaaaaaatg ccttaatcgt aagtgatatg
     1381 ccttttctat cttaccatgt ttctatagaa gatgcagtta aaaacgctgg tagacttata
     1441 aaagaaggag gagctcatgc tgtaaagtta gaaggtggct caaacgttat aaagcaaata
     1501 gaaagcattg taaatgctca aatacctgta atggggcatt taggattaac tcctcaatca
     1561 gtaaattcat ttggtgggtt taaggttcaa ggaaatacca gtgaaacagc aagacaactt
     1621 attgaagatg ctaaattaat agaaaaagca ggtgcattct ctattgtcct tgaaggagtg
     1681 cctacgaaaa tagctgagat ggtaactaat tctatttcaa ttcctacaat aggtataggg
     1741 gcaggaatta actgtgatgg acaaatttta gtttatcaag atatgttggg aatgtttgga
     1801 gattttgttc ctaagtttgt aaagcagtat gcaaatatag gagatataat aaaagattca
     1861 attaaaaatt acatcctaga agttaataca ggtgcatttc cacaagaaaa acacagcttt
     1921 tcaataaatg aatctgagtt agaaaaatta tactaatatt aaggatggta caaaatgctt
     1981 gtaaaagaaa ttaaattact aagaaatata atcaaagatt ggagaaaaca tggctattct
     2041 ataggccttg ttacaacaat gggattttta catgaaggac accaaagtct tataaaaaaa
     2101 gctgtaaaag aaaatgataa agtagtagtg agtatatttg taaatccaac tcaatttgga
     2161 ccaaatgaag attttaatag ctatccaaga gatatagata aagattttaa atattgtatg
     2221 gatagtggag caacagtagt ctttaaccca tcaccagaag aaatgtattt aaaaggtaat
     2281 tgtactacca ttaacgtttc tggacttaca gattttcttt gtggagctaa aagacctgtt
     2341 cattttggag gtgtctgcct agttgtttca aagtttctaa atatagtaac acctgataaa
     2401 gcttattttg gagagaagga tgcccaacaa ttagctgtaa ttaaaagaat ggtcaaggat
     2461 ttaaacatag atacagaaat aataggctgt cctataattc gtgaaaatga tggattagca
     2521 aaaagttcga gaaatacata tctctctgag gaagaacgaa aatctgctct aatattaaat
     2581 aagagcttaa gtttagcaaa agaagaatta gtaaaaggca atctaaatcc agagaatata
     2641 aaagaattaa taacagctaa aataaattct gaacacttag caaaaataga ctatgttgaa
     2701 attgtagata gtgagacatt acaacctgtt aagcaaatag aacattctat acttgtggca
     2761 attgctgtgt ttataggaaa aaccagacta attgataatt ttacatttga gttaaatata
     2821 tagtctataa tt
//
LOCUS       DAMJYX010000205         2805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJYX010000205 DAMJYX010000000
VERSION     DAMJYX010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.210"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(370..1077)
                     /locus_tag="PC479_003511"
     CDS             complement(370..1077)
                     /locus_tag="PC479_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2806024.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1880..2749)
                     /locus_tag="PC479_003512"
     CDS             complement(1880..2749)
                     /locus_tag="PC479_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2806025.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          985 a          457 c          242 g         1121 t
ORIGIN      
        1 aataaaatat ataaaaaaat atataaaatg gtactataaa atttttaatc ttcatcaata
       61 ccttttttgc tttcattgtt aaaactattt tttctattag tttatttctt ataaaacaaa
      121 catttagtat taaaatatat atcacacaat agtatttgca atttaacttt aaaaaaataa
      181 aaatacccaa ctgacctaag caactcgatt gggtataaat aatctaaaaa atcatttttg
      241 tataaactct ttatttaact tacattttta tttaccttct actttaattc aacttatatt
      301 tctatgcttc ttatactctc attcaactta tatttctact tatcttatac ttttataaat
      361 tcatcacaat tatttagttc tcgctatcaa agaaattgta ttaaaatact ttccattctc
      421 tacttccatc atttttttgt catttatagc atatgaatta tcacaattta tccaatcctt
      481 ccaagcttca tcatgacaat ttaaagcttc acatttctct acaatcacag actccgattc
      541 agaccaaagt ttattccacc atgtaattga atggaagttc atattatctt gccaaaatgg
      601 aatgagttca tctggtatac aatccacgaa atcttctttt aatccaggca ttgccatagc
      661 aagtattccc ccctctttta caagaggtga tatatgattg tctaaaaatc cttctttgtt
      721 tccaaaatag tgataagaat cgatacttat caccgcatca aagaaacctt ctgcataagg
      781 tagttcatgt gcctctgcct gtattggaaa tattttatca tccaatttaa actctttaaa
      841 tcttttatag ttttcagttg gttctatcca taaatctgtt gcaaatacag tagcatcaaa
      901 ttcctttgct aaaaatatcg aagatatacc cttaccacaa cctaagtcta aaatacgcat
      961 tcccttttct agttttatct tacttgttaa ttcttccaat atttttatac aattaggtcc
     1021 catcatattt ttcatgagaa agtccttatc gtatttatta ctttttgtaa atatcattct
     1081 atctttcctt tccctatcag ttattattta ttaaatttta tacgtttgat tttttatttt
     1141 ttatctgctt cttatattga gcaatctctt ttttattcat tctttaacag acaattttaa
     1201 tgtgtaattt tatccactct gtgatagtaa ctttaatgaa taatcttatc tactgtgata
     1261 gttattttag tgagtaattt tatctactcc acagcaatcg ctttaatgag taatcttatc
     1321 cactctatga tagttatttt agtgagtaat tttatctact ccacagtaat cgctttaacg
     1381 agtaatctta tctactctat aatagtcact ttaatgagta atcataattt ttatatcatt
     1441 ttagatactt actctaataa gaattcattt tctaatactt aataagtttt aattacttcc
     1501 actttaacct atatactaat taacttattt aatttagtgc atatttgaca ctttaccttt
     1561 tattatacca tttattatca taaatataat aattctattt ttttaaaata aatatttata
     1621 ataatctaaa ataaatattt ataaattcta atcaaccgta aataataatc taaaatacat
     1681 caaaaaataa actaaaaaat atcgaaaaat aatttaaaag acatcaaaac taaatcttct
     1741 aatcagcaat taaaagtcta ttttcttagt ttaaaaaata aagatagatt ttttcaaaac
     1801 actggataaa taggtaaaaa aataaacagt tagtaaaata aatttttact aactgtttat
     1861 ttttattgtt taatatgtcc tataaatcct ctaagtaata ttttccttgc aaaaattctg
     1921 attctaaacc tagattttta aaataatcaa ccatgcccat acacgcaaat ttttctgtag
     1981 aataatgagt agcacctata acattaatta aattatcttt tgctatttga tggaactcta
     2041 atactggttc aaaatgtttt aaaggcttag taaacccagt aatatataga tttataccaa
     2101 gttcagcaac ttctcttgct acaaatggat aacttcctcc ccctgctgca atagcaacta
     2161 aaccatcttt taatacagaa tctccataat catacaattt cacatcatga ccaacagatt
     2221 ttttaattaa ttccttaatc tcttctattg attttagtga tgttttacat ataactccta
     2281 cctcaatttt atcgtcatat ttacaaaatg gtttgactat ttctaaaccc atagcttttg
     2341 caaaactaac agaagttgaa tagtcactat agttgtctag tggagaatgt aatacataaa
     2401 aagaaattct acgatttttc atctcttcca tataagatag tggtatgtcg tgaaatggaa
     2461 aaccttcatc tgatgctatg tatcccattg catgatgtga aaaaattaaa atatcttttt
     2521 catctttatc tataacttgc ttaattattt ctttatcagg aaaagtagtt gtgtatactt
     2581 tattaatatt ttgagcatta tctaatacta atcctatgta tttttttcta aattctggag
     2641 taataaaatc attttcaaaa ttcataaaac tccaatcatc attaatattt ttaatattaa
     2701 attctaaatc taatttttta taaagttcaa ttgccttaat aataaccact cctttataat
     2761 aatattgaat tactacatat taatatagaa atttccgata ataaa
//
LOCUS       DAMJYX010000206         2804 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJYX010000206 DAMJYX010000000
VERSION     DAMJYX010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.211"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            15..791
                     /locus_tag="PC479_003513"
     CDS             15..791
                     /locus_tag="PC479_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2806026.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
     gene            complement(883..1635)
                     /gene="xth"
                     /locus_tag="PC479_003514"
     CDS             complement(883..1635)
                     /gene="xth"
                     /locus_tag="PC479_003514"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2806027.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1796..2746)
                     /gene="galT"
                     /locus_tag="PC479_003515"
     CDS             complement(1796..2746)
                     /gene="galT"
                     /locus_tag="PC479_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2806028.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT          978 a          477 c          295 g         1054 t
ORIGIN      
        1 aggagataaa ctttatggaa tacataattt tcgatttgga atttaaccaa ggatttgata
       61 aaaaactcaa taaaactgta tccaatgaaa agtgtccttt tgaaattata caaataggag
      121 caattaaatt ggattctaaa ttcaatataa tagatacgtt taatagctat gtaaaaccta
      181 caatttataa agacatacat ccctttatta gtcgtatgac taatattaaa agttctgatt
      241 ttaatgattc gcctactttc cctgaggtct acaacaattt tataaaattt atatcatcac
      301 aagaccctat attatgtgtt tggggagctg gagatttgaa ggaattatac aggaatatca
      361 actatcacaa gctaccatca aattccttac ctaaatctta tattaatata caacaacatg
      421 catctaaata tttcaataat ccagcaggaa aaagcattgg cctacaaaat gctatttcta
      481 tattggaact tgatgaaaaa atgtcttatc acaatgcttt aaatgatgca tattatactg
      541 ctaaagtttt tattaagata tacaatccaa gtatagtccc agatatatat ctttatacga
      601 gtataaaacc taaaactata agatattcta acaaaaaaag agttgattat gataagttat
      661 ttgatgaatt taggaaaatt ttaaatcgtg aattaactaa agacgagaaa aaaatcatta
      721 acttagctta taatatgtgg aaaacaaatc aatttacatt agaaaatgtt aagcaaagaa
      781 aaaacaaata gagtttattt agataaaaaa taggcatgta gattaaaaaa ctagttaact
      841 ttaaatttat atctacagcc caatttttat cataattaat ttttatttta tctctaattc
      901 aactggacaa tggtctgaac ctaatatttc agtgtgtata tccgcactta ctaatctatc
      961 ttctaatttt ttggatgcac aaaaataatc tattctccac cctgcattat tttttcttgc
     1021 attaaatcta tacgaccacc aactataaac accttcttta tcaggattaa aatatctata
     1081 tgtgtctata aacccactgt ctaataattt agaaaacttc tctctttctt ctttagtaaa
     1141 acctgcattt ttcatattat ttttaggatt ttttaaatct atttctttat gtgcaacgtt
     1201 catatctcca cacacaatta caggcttatg attatctagt ttggttaaat aatctataaa
     1261 gtcgtcttcc catctagttc tatattctag tctctttaat tcagattgag aatttggcgt
     1321 atatactgta acaaagtaaa aatcttcaaa ctctagagtt atcactctac cctctttgtc
     1381 atgttcttct atatttattc catacattac ttttaaaggc tctttttttg taaatatagc
     1441 agtacctgaa taaccctttc tctcagcata attccaatat tgaaaatatc ctggtaaatc
     1501 taactctatt tgcccttctt gcaatttagt ttcttgtaaa caaaatatat ctgcatctac
     1561 ttctttgaaa aagtctaaaa atcctttccc aacacaagct cttattccat taacattcca
     1621 tgatataaat ttcatactta tactcctcat ctaaacattg acacactccc catagctaaa
     1681 gatgggggct tttcaacaat tttattgtaa attcttaatt tacttttatt ttaacaatgc
     1741 tatactataa tttcatctaa aattactatt tacttaatac ctaaaaatat atgttttaat
     1801 caatttttaa cttttttgtt aaatcttcag ggctcattga agaaacatac atatttgttc
     1861 caagttcaaa tcctccaaaa ttatattttc ttgggattat gtgcatatga acattaaaat
     1921 acgattttgt ttcaatcttt gttggatgag tgtgaataca taaattaaat ggcatatatc
     1981 cattgatatt gtataacttc ttaaacaact tttcaaatat tgtagaaagc tcctgtatat
     2041 tattatcatt taactcacca aactctattt tatctttaaa tattattctt acttctccac
     2101 tatacttggt tgcatatggt ataagtacca aaaaagcttc tccattataa acaactcttc
     2161 tatctaaact tatttcattt tctattaaat catcatacaa agagctttta ttgctgtcat
     2221 aatactcctt tgaaaccaac agttcattcg taatttcagg tggaataaaa gacatagaca
     2281 atatttgagc atgtggatgc atcaaagatg ctcctgcctt tctcaagaag tttttgaaga
     2341 tactaacata ctctacttta tcatctttcg acaaatctct aaatctattt ctatacataa
     2401 aaaaaacatc ttcaaattct ttttgactca tgttataaaa actacgatta tgtttatcgc
     2461 tctctatcat tacttcatga acaccatata catctcttgc catatcatca attataggaa
     2521 atttattgta gacagatttt acaagccatc cttcttcact atctatttca aataatgcat
     2581 cagttgcatg agcttcattt cctttgcaaa atgggcattc ttcatcatat tcattagttt
     2641 cttcatcttc atttgggata tctgctgtat ctaaaggtct ttttaaccta tctgtagcaa
     2701 atataacaac atcattggta attggatcta ttcttaattc tttcataatt aacacctctt
     2761 agttaatagc ctttaatata attatatgat atttatcgta tctg
//
LOCUS       DAMJYX010000207         2801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJYX010000207 DAMJYX010000000
VERSION     DAMJYX010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.212"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            304..702
                     /locus_tag="PC479_003516"
     CDS             304..702
                     /locus_tag="PC479_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2806029.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(919..1128)
                     /locus_tag="PC479_003517"
     CDS             complement(919..1128)
                     /locus_tag="PC479_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806030.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1160..1369)
                     /locus_tag="PC479_003518"
     CDS             complement(1160..1369)
                     /locus_tag="PC479_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806031.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1668..2087
                     /locus_tag="PC479_003519"
     CDS             1668..2087
                     /locus_tag="PC479_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806032.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2315..2770
                     /locus_tag="PC479_003520"
     CDS             2315..2770
                     /locus_tag="PC479_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806033.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
BASE COUNT         1067 a          347 c          348 g         1039 t
ORIGIN      
        1 tctcttaatc tttttctctt ttacagacaa tcaaaaagca atcttagttt atcgaaataa
       61 atttatacaa tctattcata caccccttta catttatata acaattaaac ctattgttat
      121 atatacaaat caaaataaac catatatttt caactataat atgttaaaat catattatga
      181 gcatattaat ttaagcaaaa tgtcatttag actttaattt agtagtttta tattataaat
      241 ttatatattt ttaattttaa atgtcattta gacattactc ataaaaagaa aggagtgtat
      301 tttgtggata ctttaggaaa aagaattgct tatttgagaa actccaaaaa acttactcag
      361 cgcaaactta tggatatttt aaaatttgaa aacttaggca agtatgaaac aggagataga
      421 aaacctaatt gtgatatact aatgtcaata gctgattatt ttaatgttac gactgactgg
      481 cttctctatg gtaaagagaa ggtaaatgtc aatagtagtg taaaagaaga taaagaagat
      541 tacttacatg ttactaatga tgaaatgacg atattaaatc tatacagaca actaaacgaa
      601 cgtgataaaa taaaaataga aggaatactt gaacttaaga tatctgaata taaagactta
      661 aaaaagcatt cctctaataa taatgaagat aaaatggttt aaacatacat aatttgatac
      721 gattttacat catctattaa ccttcttttt ttgtagtata ttactaaaat atggatattg
      781 gattatttaa aaacaagtat agtctaattt caagcacatt tataatattt agattatctt
      841 atccatattt tagtttaagt actttgaaaa atataataca tctctatgta taacttatat
      901 tcctaagaaa tgcattactc atgtttttaa ttcttccaac atctttctat acacaattgt
      961 acatccttca taattcccct ctttaagaag gattgaacat gtactagaat atttagttaa
     1021 agtatttaat ctttccatat ttaagctttt tatattactg agtgctgtaa taaaatcatc
     1081 aattgctcta caacactcta taaccccatc tgaatccaat ttacacacaa gaatattcac
     1141 ctactttata tactatttat taatcatctt ctgaatattc tatattaagt aattgataaa
     1201 ttgatgattt atatttttta cctgttcttc ttccatttaa aatagctgac aaatagtttt
     1261 cattaacacc tattttcttc gctaattccc tttgagtcat atttaattca ataagtcttt
     1321 gcttgacttg cactcccaaa aaaggtatcc atttttttcg agcatccatt tatcaacttc
     1381 ctttctaagt gatttatatt tacttttata ttattttagc ttacacaccc cctctattta
     1441 gtatatattt ttaatatgta cacatattaa gtatataaat ttatttaata ctaaataaga
     1501 ttttttgatt actttgtgct aaaattagga tgtaaattcg tatcattaag ttaattataa
     1561 agtcattttg accttaagtc aatgcttttt atgtcatttt gaccttaaat ttgaaactat
     1621 ttacatctta aaaagtcttt ttgacattaa ttgaatggag agtgtttatg gatactttag
     1681 gagaaagaat tgtttatttg agaaaagcaa aaaaccttaa acaatatgaa ctagaagaaa
     1741 tgttgggctg tgacaactta agtaaatttg agagaaacat tagaaaacca aattatgaaa
     1801 tattaaaatc tatagctgag atatttaatg tttcagtaga ctggctatta aatggagata
     1861 atctatcaca taaatcagat ttaatttgcg attcctcctc aaactatcca cttaatagca
     1921 taaactctaa tgaaataaag cttttaaaca attttagaaa gttaaatgat tatgataaag
     1981 ccaaaataga aggtatgatt gaattaaaac ttcatgaata tgaaaaagag aaggatttag
     2041 gaaaaattga gtataacaaa aataaagatg agaaaataga taaataattt ttatgttgct
     2101 tttgatacga ttttacatca tttctttgtt ctaatatttt tatttcattt attttctttt
     2161 ttataatttt atgataaaat aattagtata ttttgcttta cttgaatgta attatttata
     2221 caaaagtcaa acataatata aatgtagtaa atttttagtt tcaagtaata tctcgttatt
     2281 ataactaaat atttattaaa attaggagga taaaatgggc ttaagagaca aattcgcgca
     2341 atcatttgct agatcaaaaa ctatgagtgg ccctgagaaa aaggctaatg aaataatggg
     2401 taaattgctt cttaagaaag ctatattacc tatcgtttta atgttcgtga taataatcgc
     2461 tggagctatg cttaaaatca attcttgggt tacacttggc ataaatttag ttattgctgt
     2521 tggtgctttc ttctacataa gaaattctag taaaaagtat cagaacttta aaccttatgt
     2581 tggcaatttg attagtttag agaaaaaagg taaaaaagag tacgttgcta ttataaaaca
     2641 aggtaagtta ccagttaagt tacaaatagc ttatggtggt gaagaccttg aacatgttaa
     2701 gaaaaatcaa atggttcaaa taagttataa tccagatgct aaaatagcta ttttggttaa
     2761 tagacaataa tttaataaaa aaaaggagtt gtcttaaaat t
//
LOCUS       DAMJYX010000208         2793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJYX010000208 DAMJYX010000000
VERSION     DAMJYX010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.213"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..597
                     /locus_tag="PC479_003521"
     CDS             <1..597
                     /locus_tag="PC479_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2806034.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            694..1731
                     /locus_tag="PC479_003522"
     CDS             694..1731
                     /locus_tag="PC479_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2806035.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2540..>2793
                     /locus_tag="PC479_003523"
     CDS             2540..>2793
                     /locus_tag="PC479_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806036.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT         1064 a          361 c          426 g          942 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaaatt actttagata gtactactat aacaaattta
       61 gctgagtata aaagaactca aatagtaagt agagtttttc aagacccaag ccttggaact
      121 tgtccttcta tgacggttag agaaaattta tctctagcac ttaataaagg gaaattattg
      181 aacttaaaaa aatgtcttcg tcataaaacg aatgacttgg aacacttact tgaaggaatt
      241 tctttggatt taaaaaaata tttagatatt aaagtacaat atctttcagg gggtcaaaga
      301 caatcactat cactaattat gtctagctta gcaagtccta aggtcttatt actagacgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa tagagttaac agataaaatc
      421 gtaagagaga aaaatataac tacacttatg gttactcaca acttgaaaca cgctcttcaa
      481 tatggagata ggttgataat gcttcataag ggtgaagttg tattagatgt aaaagataaa
      541 gaaaaaaaag atttaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtataagaa
      601 attaaaatct tagttttaat cagaaatatt tataatctat catatgaata tatgaacaaa
      661 tataattatt aaattttaag ggaggaaaat aaaatgataa aaagtaaaaa aatattaagt
      721 ttaattatag caggagtact tggtgtatca atgcttactg gatgttctca aaatgatggc
      781 tccaatgctt caaatgaaaa taaagaaaca gatagcaaaa aacaaaaaaa tataggtatt
      841 actcaattag tggaacatcc atctctggat aaagcaaaga aaggattcat caaagcactt
      901 gaagataaag gctataaaga tggagataat ataaaaatag atttccaaaa tgcacaaaat
      961 gatatgccta ctacacaaag tattgctagt aagtttgtat ccgacaaaaa agatttgata
     1021 tatgctatat ctactccatc tgcacaagca gcttataatg ctactaaaga tattccaata
     1081 ataatgactg ctgttacaga ccctgtagaa gctggacttg ttaaatctct tgaaaaacca
     1141 ggtggaaatg tttctggtac atctgattat ctttcaattg ataaaacatt agaattggtt
     1201 aaaacattga ctccaaaagc aaagaaaata ggggttatat acaatactag tgaagttaat
     1261 tcaaaaatcc aagttgattc tctacatgat tatgctaaga aaaataatta tgaagtagtt
     1321 gaaaaaggaa tcagctcttc aagtgaagtt aaccaagcta tttctagttt agttggtaaa
     1381 atagatgttt tatatgtccc tactgacaat ttaatagttt cttctatgcc aatagtttct
     1441 aaagttgcta atgaaaacaa gattcctata atagcctctg aagaaggttc tgtatcttct
     1501 ggtgctttag cttgttgtgg aatagattat gaaaaactag gttataaggc tggagaactt
     1561 gctattgaag tattagaagg aaaatctgtt ggcgatatac cagttactac attagatgaa
     1621 actgaaataa taattaacga agatacacta aaagcacttg acatgcaaaa gttatcagca
     1681 gataatataa agtatataaa gtcagatgaa aatgcaaaat ctacaaaata aaacttatta
     1741 atttcaaaaa ataggtaata tgaagtaggt aatatgaaaa ctttatataa taagtactct
     1801 tatccaccct aaccctttga aaattgtgct tattgtagca atttgaatat tgcatataaa
     1861 gttattcatt ttccataaaa gtcgtctata ttgacgactg attatgaaag ttatataact
     1921 ttctaatcac tacttgagct gtccaaaaga attttaatat tctccagaca gctttttttt
     1981 atgatatttg attgatatac atgtctagtt attccatatt tagtaaatat tttgataaaa
     2041 tttccacatt cacttataac ttaggataac gtttttttca aacaatacat taatttaaaa
     2101 gtattctgag tatacattat ttagtatttt aattaaattt agcagttgac ttttattatt
     2161 gtataattta taatttataa caagtcgaga atacatataa ttttctgact aattaaaata
     2221 ttaaaaggga gagataatta tgaatacttt attaaacaat agaaaatatt attattatta
     2281 tagccactat ttttagggtt tgtacctacg ttttaacata gatacagacc ccttaagact
     2341 actaaaattt tgggattgta tctatgttgg ctatattgtt tataaagttt tcatctaact
     2401 atataagagc cacatagatt aaactatgtg gcttattcgt gtttttacca catagttttg
     2461 tacactatgt ggttttttta tacacaaaat tagcaaataa ttaaaattat aaaaatattt
     2521 tcaaaggagt gttttattta tgtcaggtat tatatctgta atgacgcaaa gtttaatttt
     2581 atcgattatg gcactggggg tttatataac ttacaaaatc ttagattttc cagatatgtc
     2641 tgctgatggt agctacacaa tgggtgcttc tattgtagca ttttctctta ctaatggtat
     2701 ttctcctgta gtagcaactt taatggctat cttatgtggt tgtactgctg gtcttgttac
     2761 agggattctt cacattaagt ttaagatatc taa
//
LOCUS       DAMJYX010000209         2778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJYX010000209 DAMJYX010000000
VERSION     DAMJYX010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.214"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(342..1238)
                     /locus_tag="PC479_003524"
     CDS             complement(342..1238)
                     /locus_tag="PC479_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2806037.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1379..>2778
                     /locus_tag="PC479_003525"
     CDS             1379..>2778
                     /locus_tag="PC479_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2806038.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFY"
BASE COUNT          947 a          382 c          326 g         1123 t
ORIGIN      
        1 tttaggatgg atttaaattt tctataaaaa tcatactatt ttagctgttt acttgcttat
       61 ttaatagttt tatttctttg attaaatatc tttcagaatc taagccagat gtctcaaaaa
      121 aactgattta taatataatc attttttctg ctattcttat ttttccaatt ttagtttcat
      181 attaaaatat acttttcata gacactacat tctttcatac tcattttcac atttaactat
      241 acaagtagtc ttactcattt atgtactata ctttattaat atttattaag aaaaactcaa
      301 agatttaaaa atatatattt tctataaagt atatttatga attatttcat atcttctttt
      361 aaaataacat tactaacttc tttatgttca tttctatact tagttggaga tatattataa
      421 ttccttttaa ataagcgact aaaataattt atgctatcaa agccacatct catagcaatt
      481 tctgtcatat ttaaatctgt ttcctttaaa taatatatag ctttatcaag cctgattcca
      541 tttataaaat cgattgtagt cttacctgtt atttgcttaa atgttctaca aaagtgatgt
      601 gcactcaagt tcacatgact agcaagttct tttaatgtaa ttttatccat atatttttct
      661 tctatcaaat caaagataca ttcaaatttt tctaaagaat actttctttt tataaattca
      721 tcttcagaat atattttatc aatatgactt cttaataaga gtactattaa ttgatagata
      781 ttagctttta tagataactc atatccaact tctttattgt gatattcatc aagtatagtt
      841 gtaatacatt ttaaaatcag agaatcattt tgaattaaat tattaaatac aatatgattt
      901 aaagccaatg gtgctaagta cttggtttgt aacaaatcaa cttgattact aaaaaggaat
      961 gtgggttcaa tacgaatact atagaatttt aaattatctg ataagctttc taaataatgt
     1021 aattcattac tgtttgctat tacaatattt ccaggcacta ccttaaaatt atccaatcca
     1081 cattcaagat atgctatacc atcaataaaa taatatattt gtagatgctc atgccaatgt
     1141 cttttaaagt ttgctccttt tttaatgtca tcctttaaaa aaatatctat aaagttttct
     1201 tgacctttcc tgttaaaata tgtattttta tccttcatta ttcccccctc atcaaaatag
     1261 tgctaattat aagcaatata gttattgtat tcattatttc atatcgctat tatataaaat
     1321 gtaactatca ttgtaaacat taacataatg cagtcattat aataaggagg taataaaaat
     1381 gacaaactct tttaaaaaag atgataatag aaaaaaaatc acagttttat ttgtagtcat
     1441 tgccatgact tttatggcaa cattagattc tagtattata aatgttgctc tcccagttct
     1501 tgcaagcaaa ctaaatgtat ctctagcatc tattgaatgg gtaatagcaa gttactcaat
     1561 tattatttgt tcaacactat tattttttgg aagacttgga gatattattg gtaaatcaag
     1621 agtatttcaa gtaggtacta ttctctttac atcagcttca ttactatgtg gattaagtaa
     1681 ttctttaact ttacttatag tttgccgctt tattcaagga ataggcgctt ctgcctacat
     1741 ggcaaacaat catggaatta tcacagaact atttccaaaa gaaagcagag gaaaagccct
     1801 agggatttta gttactgcag ttgctattgg aaatatggtt ggaccatctg tgggaggatt
     1861 tattttgtca atatttgatt ggaatgttat attttttata aatattccaa tcggtcttat
     1921 tgtaatcttt ttaaatacaa aatttcttcc taatagtaaa aaatcttcag aaaatatgga
     1981 taaaactggt gctatacttc aatttttagg aacaacatta ttttttagtg ctctaattag
     2041 tgctcaacaa acaggattac taaatccata tatattaatt gcactattat tatcaattat
     2101 ttttattata ttatttttga tattagaaaa aaaacatcct caaccgcttc ttgaccttga
     2161 aatttttaga aattttaaat tttcattgaa tttaatttgt gctcttacat cattcatttg
     2221 tattgcatca tcaagtattt taattccatt ttatttacaa agtactatga aacttccccc
     2281 tattcaagca ggtttattta tgatactatc acctttgatt ttagccattt tttcacctat
     2341 atttgggaat atatcagata aaattaagtc agaaaaaatt attttaattg gtcttctcgt
     2401 tatgagtttt ggattctttc taatgtcaag attgaaagaa agttctgccc ttattttatt
     2461 tgtaatttat atatcgataa tatcaatagg acaagctata tttcaacctg ctaacaatgc
     2521 tttgataatg tcaagctgtt ctagaagtaa gctaggagtt gtaggaagca taaattccct
     2581 tgtaagaaat ttaggacaag ttataggtat tactatttct acaacacttt tatataattt
     2641 tatgagtatt aaagctggat atagggtcaa tgattatgtg attaataatg ataaaatatt
     2701 tgtatttgga atgagaaatg tttatattat tgttacactt gtttgcttaa taggtgcaat
     2761 attaattggc ttttattt
//
LOCUS       DAMJYX010000210         2754 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJYX010000210 DAMJYX010000000
VERSION     DAMJYX010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.215"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            196..1017
                     /locus_tag="PC479_003526"
     CDS             196..1017
                     /locus_tag="PC479_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2806039.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    1096..1307
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1394..2710
                     /gene="argH"
                     /locus_tag="PC479_003527"
     CDS             1394..2710
                     /gene="argH"
                     /locus_tag="PC479_003527"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2806040.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         1129 a          246 c          543 g          836 t
ORIGIN      
        1 ctataaattg aaaatattca gaaaaatact acaagaaacg atttattttg ctcttaaaat
       61 aagttaaatg aatgttatta attttgtaaa ttgaaatagt ttaagtataa agttatgata
      121 taatctaata agggtaattc tttagattat attaaggaaa tataggtata caataaaaag
      181 ttatggagtt ggattatggg atataaatta attgtaacag atatggatgg aactttatta
      241 ggcaataatc ataaagttac agatgaaaat aaaactgcac tacaaaaagt tataaaaagc
      301 ggtattaatg taactttagc aacaggaaga gccttcgatt cagctaaatg caatgtagat
      361 tttcttaaag aggacatgcc aataatagct tgtaatggtt cattaataag agagcaaaat
      421 ggaaatataa tatattcaaa taagattgat acaagaacat gtttaaacat tttagatgta
      481 ttagataaat acgatattta ctaccaatgt aatagtatag attctatgct ttcgaaaaaa
      541 atagaaggac gtgaagatag attaagtgta tttttaggct ctgaaacaga agtaattgta
      601 aaagatgatt tgagagaaga aatctttaaa aaagatatac taaaatttgt aatcatagaa
      661 gaaaaaaatc catctatttt ggatgaaata agaaaagaat tgagaaaagt tcaaggaata
      721 aagataacct cttcatggcc aaacaatatt gaagtcatga atgaaggtgt tgataaaggt
      781 aatgcagtta aaattttagc tgaaaaaatg aatatagaca gagaagatat aattgcattt
      841 ggagacaatt acaatgacat agaaatgata aagtttgctg gtcttggggt agctatggga
      901 aatgcagaag aattgattaa acaagaagct gattatgtta cagatacaaa tcaagacagt
      961 ggagttgcaa aggcaattta taaattccta gagttaaaag aatcacttag ctcttaaaac
     1021 agtatataat aggaaaaaat aggttgacta ttttgtagat agtaattata atacaaatag
     1081 ataaaatata aaaatttaga ttcagtgaaa aagagagtag atatagagaa aatctcagcg
     1141 aattggggga tggtgtgagc ctaatgataa cactttatat tgaaaagaac tttggagaat
     1201 actacttgaa attcagtatg gtagtaggta atctcgcctt aagagaatag agtgggtaaa
     1261 cattgtttac caactagagt ggtaacacgg aatatttcgt ctcttggtct ttttgaccaa
     1321 gagatttttt agtttcattg agttacaaga cttataatta aaatacaaaa aattaaaata
     1381 tgaggtgaaa gttatgaaat tatggggtgg acgctttaga aaagcggaaa atcaacttat
     1441 ggaagaattt aataaatctt ttggctatga ttgtgtactg tacaaaaaag atatcgaagg
     1501 aagtgtagca catgttcata tgcaagtaaa atgtggactt ttaacagaag aagaaggaaa
     1561 gtctataaca gaaggtttaa aagggatact tgaagatgta gaaaatggaa agttagtatt
     1621 ggatggagag tatgaagata tacatagttt tactgagata aatttaattc aaagaattgg
     1681 agatgttggc aagaagcttc atacagcacg tagtagaaat gaccaagttg cagttgatat
     1741 gagattgtat gctaaagaaa aagcaaatga tttggtggat ttaatatctg aatttaaagc
     1801 tactataaaa gatgttgctg acaaaaaccc agtgatgatg ccaggctata ctcatcttca
     1861 aagggcacaa gtagtgacat ttaagcatca cttaatggca tattatagta tgtttgatag
     1921 agatgagaaa agaataaaaa atgctataga aatattagat gaatcacctt taggctgtgg
     1981 agctttagca ggaactacgc atgatataga tagaagtata acttgtgaac aattaggatt
     2041 taaaaaagta gtagataatt ttatggatgg agtaagtgat agagattatt tactagagct
     2101 aatgagtgat ttttcaatta taatgatgca cttaagtaga ttaagtgaag agttgatact
     2161 ttggagttct caagaatttg gatttgttga gatagatgat ttatatacaa ctggaagtag
     2221 tataatgcct cagaagaaaa atccagatgg tgcagagctt ataagaggta agactggtag
     2281 agtatatgga aacttatttg gtctttttac agtaatgaaa ggaataccat tagcatataa
     2341 taaagacatg caagaagata aagaaggttt tttcgatagt gtacatactc ttgaaatgtg
     2401 tatacaaata atggatagaa tgatagccac actaaaggta aatgaggata aaatgaaaca
     2461 agcagtaaaa aatggttttt taaatgcaac agaagttgcg gattatttgg ttaaaaataa
     2521 tgttgctttt agagatgcac atggtatagt aggaagtatc gttatatatt gtgaagataa
     2581 taaaaaagca atagaagatt taacattaga agaattacaa aagttttcag atgcttttaa
     2641 ggaagatatt tatgatttta tcgactatga gagtatatta aataagggaa taaaaaagaa
     2701 tttaaaataa tgtatttatt tttaaaagct gtaaatttga atgttatcat ttaa
//
LOCUS       DAMJYX010000211         2724 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJYX010000211 DAMJYX010000000
VERSION     DAMJYX010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.216"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(259..1071)
                     /locus_tag="PC479_003528"
     CDS             complement(259..1071)
                     /locus_tag="PC479_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2806041.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(1147..2565)
                     /locus_tag="PC479_003529"
     CDS             complement(1147..2565)
                     /locus_tag="PC479_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2806042.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT          957 a          487 c          276 g         1004 t
ORIGIN      
        1 acatcaatat ttatatatat tttgtatatt aaatacaatt atttataaag tctttaataa
       61 aaatttataa atagagcatc tttagaaaac tattataata aaaaaataag aggctgtcta
      121 aaaatataga taagcctttt ttcataatcg taattataaa aatatacttg atttcatatg
      181 aaaaagagtg tctcataaac taaaatgttt actttgagac accacctttt ttattcttat
      241 ttatactttg tagacatatt ataacatgcg tttcactggt atctgaactt ctgtcaacca
      301 gtcttcttca ctctctttat tccatatacc atctatataa gattctctag gatttccaac
      361 cactttatat ttattatcct ctatccattt aaatgcatct gcgtaagttt ttcctaatgt
      421 tgaatatgga ccattgtgta atatacaaac tgcattttca actgcatcta attctttaaa
      481 ttttaatata tccgtatctt cctttaaatc atttatagct tgacacattt caacatccat
      541 atcagtctct ctatattcac catcatgata tatattaaaa caataatcag gaactgcaca
      601 tgtacaccct gttttcatca tttcttcagc cattacattt ggaaatagat taaaaagttc
      661 atcatacccc tttaacactt ttctcataga agcaacttta acttttggta aagacttaat
      721 aattacatca ttcatattga cttctccttt caaattcata aggtaattac gtagtttaat
      781 tattttatat tcttcacttt taattacatc caacatttct ttttctttat tcattaaaat
      841 attttcaact tcttcaggat tttctacagc tttttttatc tcagctaatg ataaacccat
      901 ttgttttaaa gttattatct gatgtaattt aggtaattgc tctgttgtat aatacctata
      961 ttttgtaaat tcatctacat attctggttt taacaaccct atatcttcat aataccttaa
     1021 tgtctttgta gttattttat ttattttaga aaacattcct atagaaaaca tctcatcacc
     1081 tgcgcattag atttgattaa tttaagttta aactttcccc ttaggttaaa gtcaatacaa
     1141 aataaattat aattgtatta attcttcaat tctatctcca ctcaatacca aaatatcccc
     1201 tgtttttata atggttgaac ctttaggtat taaaacctgt tcctttcttt ttatcataac
     1261 tattaatata tcgtctggta tattagaatc tattatactc ttattagccc atttattacc
     1321 ctcatcaaca tttacttcga taagtttaga gctaatttct ccatcataat cattaaatgt
     1381 ttttaataca ggtgtattat catcaactaa atccaacttt ctggctacag ttggtatcaa
     1441 tgaaccttgt attcctactg aaagcaacgc tataaaaaat attatatgga atatgtcatt
     1501 ctctatggct actccttgag taacagcata aattgcaaaa actactgaag ccgcacctct
     1561 aagacctacc cacgatatga ataatttttc tttatttgag aatttaaatt ttgctagaac
     1621 tataaatgtt gctaatggtc ttgcaataac tatcatgaat atagatatgg ccattgctat
     1681 accaaataca tttggtaact tagaagggaa agataacaat cctaacataa agaataacat
     1741 tatttgcata atccatgaaa ttccatcaaa gaaatgaacc aaactttttt taagaggtat
     1801 cctactattt cctataataa tacctgaaat gtaaacactt aaatatccat ttcctccaag
     1861 atactcgcta aatgcataag aaagtattgc aatagctgtt ataaatatag tataaaatcc
     1921 ttctatctcg aagtttgaat gtcttaaaat ataaacagat aattttgcta gtataaatcc
     1981 tatagctaac ccaaaagcta tttgttttat taccattggt gcaataagtt ctaccccaga
     2041 attattcatc aatgtcaaaa gaattagtgt cagcatatac gctattgggt cattacttcc
     2101 actttcaact tctaataatg atgcaagtga accctttaag tttagttttt gagaacgcaa
     2161 cacagcaaac acagatgcag catctgttga agctgcaatt gccccaatta ataaaccttc
     2221 aagtaaacta gttcctatta ccatgtggca aaacatacct gtaaccccag ccgtaattat
     2281 tacacccagt gaagacatta atatagaagg tatagcaaca ggtttagcca tattccagtt
     2341 tgttccaaac cctccaaaaa acattataaa tattagtcca atagaacata attgttttgt
     2401 cagttcatag ttatcaaaat agattcctac catcccatca gacccaaata acatccctag
     2461 taagataaaa atcaataata ttggtactcc aaatttatac aataccttgc tagaagttat
     2521 acaaatcaat agtactaacc cacttataat cattaattct gtcatagcat caactccatt
     2581 tcatataaat tttgtagatt aatttacatc tacaatttta ttacttattc aaatataaca
     2641 tgacataaat tttataagtt atggtacata aagttcaaca aaaatttcac aatacattaa
     2701 aatgtgagtg ttatacttaa attt
//
LOCUS       DAMJYX010000212         2711 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJYX010000212 DAMJYX010000000
VERSION     DAMJYX010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.217"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            1122..2393
                     /locus_tag="PC479_003530"
     CDS             1122..2393
                     /locus_tag="PC479_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2806043.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1063 a          339 c          348 g          961 t
ORIGIN      
        1 actaaagtca atgttatctg gtaattattt actacaaatg ataaaaaata attatatttt
       61 aaaggataaa atgctgtata aattattggg gtattttacc tatacttgtt acaaatttac
      121 ataccactta gttaatataa aacaaatttc aaagagattg gacaacgaat aaaaattgca
      181 atttatatac catatagtta atataaagca tatttgtgtc gatatatagt tctaactaag
      241 tataagattt acatacctca tagttaatat aaaacacata ttttacatgt ttgaagattt
      301 tatcagaaac cctatttaca taccgcatag ttaatataaa actattaata gtgctatgtt
      361 gtttatttgc attaacaaga tttacatacc acatagttaa tataaaacat gtttagcagg
      421 agttatgatg ataaaacaca ttatttattt acataccact tagttaatat aaaaccccaa
      481 aataaactaa gtattttcag tacttacaca catacaactc tctcaaattt gcagtgaacc
      541 atgagtagtg caattgataa catttatcat acaccctcaa tcccttacat tgcaactgtt
      601 aaaccctact ttatcaaaaa tatcgctcac tgcaaaattt atatattttt attatatcat
      661 aaaattcaaa ctataaaatt aaaatggaat aatttatcaa tttaagaaca cctacatatt
      721 tgtaaatact tactatatta tctattctaa tagttcttct tctagctaga actaatataa
      781 aaatcatctc tgaaagatta ggacatacta atataaaaat aactatgaat agatattttc
      841 ttgttttaaa cgatatggat aaaaaaactt ctgaaaatat aatcaaagtt ctctttaaat
      901 aacattccat tttatgtaag taggcattat atttttctta aatgtcactt aaatgtcagt
      961 tatatataat aatagtaaac aactgtattt cactaatatt tgctattatc taatagtatt
     1021 tatagtttag atttgatata atacatgtgg taaaattttg atttgtaaaa caaaatttta
     1081 cctacatata ttaaaacaaa tttaaaacta aggagaccta tatgttaaaa gtactaaaag
     1141 gaaatataat ttttacaaaa acttcagaaa ctttcacagt atttgaaaat agttatatta
     1201 tgctggaaaa aggtaaagta aagggaatat ttaaagagat acctaaggaa tacaaaaacc
     1261 taaaagtagt agattacact gataagctta taatacctgg tatgaatgac ctccatgccc
     1321 atgcctctca atttaaaaat cttggaatgg ctatggataa agagcttttg ccatggcttg
     1381 aaacttatac atttccagaa gaatcaaatt ataaagatat agaatatgca acaaaaatgt
     1441 ataaaaaatt tgtaaaagaa ctttggaaat ctggaactac aagaatagca gtgtttgcaa
     1501 cagtacataa agaagcctct atgaaactta tggatatatt caaagaagtt ggtcttggag
     1561 catacgttgg taaagtaaat atgaatatga attgccctga tactttatta gaaaacactc
     1621 aggattctat tactgataca gaagaaatta taaataagta tagtgataca gattctattg
     1681 taaaaccaat aataacacct agatttatac caagctgtac aagtgaactt ttaaaaggtt
     1741 taggagattt atgtttaaaa tataatattc ctattcaatc acacttatct gaaaattatg
     1801 atgagattga atgggttaga agtctagaac ctgaatctaa attctatgga gatgcatata
     1861 atagatataa tttatttggt caaactccta ctttaatggc tcattgtgtt cattgttctc
     1921 aggatgaaat agatttaatg agagaaaata atgttatggc agttcactgt ccagcttcta
     1981 actttaatgt tggtagtggt gctatgccta tcagaaaatt aatcgataat aatattagat
     2041 tagcacttgg atcagatata agtggtggac atacattgtc aatatttaaa gcaatggtat
     2101 ctgcaataca attatctaaa ttatattggg ttaactctgg taagaagtat aatttcttat
     2161 ctttatctga ggctttttat attgcaacca aaagtggtgg aagtttcttt ggaaaagttg
     2221 gtagctttga agaaggttat gattttgatg cattgattat tgatgattca aatttaaatc
     2281 atgacaatta ttctatactt gaaagacttg aaagatttat atatgttggt gatgatagaa
     2341 atatcattca tagatatgta tgtggaaaac taattgaaga acctaatata taattataat
     2401 aaaaatttta attatatatt atggttaatt tatacaacta taattaataa aagagtatat
     2461 tttaaatcta aaagctatag gttgaaacat actcttttta ttttgtttat ttatagcttt
     2521 agttattaat catatataaa tctacaaaac gtattcctag taacttacat tttttgatat
     2581 ctacaatcat aagactatat tattattgga ttcagacctc aaaaatattg ccttctgaac
     2641 attattatat tcaaagctat gttaatttac aatgatatat ctaacatgta ctgtaattat
     2701 caaacacttc t
//
LOCUS       DAMJYX010000213         2646 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJYX010000213 DAMJYX010000000
VERSION     DAMJYX010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.218"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            105..608
                     /locus_tag="PC479_003531"
     CDS             105..608
                     /locus_tag="PC479_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2806044.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            655..1614
                     /gene="moaA"
                     /locus_tag="PC479_003532"
     CDS             655..1614
                     /gene="moaA"
                     /locus_tag="PC479_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2806045.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1618..2088
                     /gene="moaC"
                     /locus_tag="PC479_003533"
     CDS             1618..2088
                     /gene="moaC"
                     /locus_tag="PC479_003533"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2806046.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2124..2555
                     /locus_tag="PC479_003534"
     CDS             2124..2555
                     /locus_tag="PC479_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2806047.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1103 a          242 c          516 g          785 t
ORIGIN      
        1 aatatgttat attgctgata gaaaagagaa taaaagaaaa taggttttat taaaagagat
       61 ttttatttat aggacttaat caatgtatag aggggtgaga acatatgttt aatgttgcaa
      121 ttataacact tagtgataaa ggatatgaag gaaaaagaga agatataaca ggaaaaaagt
      181 taactgaatt tgttgaaaat acaggagttt ataaagttac tgaatatgta cttataaaag
      241 atgataagga aatgctaaag gaaaatatta taagactttg taatagtaat aaaatagatt
      301 taattcttac aaatggaggt actggttttt caaaaagaga tataacccca gaagcaacta
      361 aggaagtgtt ggaaaaagaa ataccaggtc tgagcgaata tatgagaatg aaatccacag
      421 aaattactaa aaaggccata ctaagtaggg gtgtgagtgg aataagaaat aattctataa
      481 taataaattt acctggaagt cctaaaggtg cagttgaaaa tctaagtttt ataattgatg
      541 ttttagacca tggcatagaa gttttaagag gagaagctac agaatgtgcc actaaaaaaa
      601 gtgaatagta tataatctag atagataaaa aatagacaaa gaggtagata aagtatgatt
      661 gataagtatg gaagaaaaat agactacctt cgtatttcac ttacagataa gtgtaattta
      721 aggtgtgtct attgtatgcc accagatgta aaatttgata aaaattatat aaatgaaaat
      781 ctaagctttg aagactacaa gtttattata aaggctatgg ctgaacaagg tataacaaaa
      841 attagattta ctggaggaga acctctttta tatcctaaat taaatgagct tataaaattt
      901 acaaaagaag aatgtggaat aaataacata ggaatgacca ctaatgctat tggtctttct
      961 gatagaattt ttgagcttga aaaatgtggc ttaaataaag ttaatataag tttagattct
     1021 ttgaaggaat ataagtataa atccataaca agaggaggaa acttaaaaga tgtcttaaaa
     1081 tcgattgaat cttgtttaaa tttaggtata aaagtaaagt taaattgcgt tgctataagt
     1141 gggtttaatg atgatgagct taaagatttt atgtttttaa cagttaaatc accaattgat
     1201 gtaagattta tagagcttat gccattaggt gaggctaata gagtttataa gaagggttat
     1261 tttaatgtaa aagaaatgat tgaaaaaata tatggtttat ataaagtcaa aaatgatgaa
     1321 aaaagtacag cagaatatta tatgtttaaa ggagctaaag gaagaatagg aattataact
     1381 ccattaagct gttcattttg taacacttgt aatcgtatta gacttacttc tagtggtgca
     1441 ataaaattat gtctacattc aaaagaagaa atagatataa aaccattttt acataaacca
     1501 ttgttattta gagaatctat gaaagatata ataaaacaaa aaccagaaaa acatcattta
     1561 atagaaaata aatgtagtga tacaaatagg cgtatgtatg aaattggagg ataagtaatg
     1621 ttaacacata taaatgaaaa agggtatgcc aagatggtgg acataagtga aaaagatgat
     1681 actaaaagaa cggccgtagc tacagctaca attactctta gtaaggaagc attagaaaaa
     1741 gtaaaaaatg gtcagataaa aaaaggagac gtattgtctg tagctcaagt tgcaggtata
     1801 atgggagcta aaaatacatc ttcaaacata ccaatgtgcc atcagattaa attagttgga
     1861 tgtgatttag aatttgatgt agatgattct atgtctgcta ttaagatata cgcaacctgt
     1921 aaatctcttg ggaaaactgg agtagaaatg gaagctttaa atgcatgttc tttagcagca
     1981 cttacaattt atgatatgtg taaagcagtt gataaaaaaa tggttataag taatatccat
     2041 cttgtggaga agactggtgg aaaatcagga gattttaagt tcgagtaaat taataatata
     2101 ttgggttaaa atttggagga aatatggcga aggtagtgtc aataaatata agtgagaaaa
     2161 agggtgtaat taaagttcct gtacaatgtg cagagctaaa aataaatcat gggatagttg
     2221 gagatgcaca tgcaggaaac tggcatcgtc agataagctt acttggaaaa gaaagcatag
     2281 ataaaatgaa aatgaaaggc tttgaccaat taaaatatgg agattttgct gaaaatgtaa
     2341 ctacagaagg gatagaagtt ttttccctgc ctgttggtac aaagttaaga atagggcaat
     2401 gtgaagttga agtaactcaa atagggaaaa aatgtcacaa tggatgtgaa attaagaaat
     2461 tgacaggtga ctgtgtgatg ccacgagaag gaatttttgt aaaggttcta aaagaaggaa
     2521 ttataaatgt gaatgattca atagatgtaa tctaatgaag tcactattaa aatataatta
     2581 taagtaaata ctattaagat ttaaatatat attttaagag agaattaatt ttaataaaat
     2641 atttta
//
LOCUS       DAMJYX010000214         2639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJYX010000214 DAMJYX010000000
VERSION     DAMJYX010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.219"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            273..2057
                     /locus_tag="PC479_003535"
                     /pseudo
     CDS             273..2057
                     /locus_tag="PC479_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1155 a          366 c          469 g          649 t
ORIGIN      
        1 ttttatacaa aaccctattt tttataacaa ttcattaaca taaaaatcaa acttacctta
       61 aaatataaat ttacaaattt ttcaatcaat aagaaaattt ctttattcct tacataatga
      121 ctgcaatgta acaaaataat tacaaattaa ttatgaaatc attatagtta gaaacctctt
      181 taaaattaga tattaagtat cctttagaat tagaacggga acaaagttaa aaaattaagt
      241 aatatcaaat ggacaagggg gaagaaaata aaatgaaaat accaaagaag attgctgcaa
      301 tgcttacagt tacaatgata gctggaagtt caacagcagg catagctagt gcacaaactg
      361 tggcaacaaa tttaacaggg caagaaagat atgaaactgc tgttaaaata agtcaagatg
      421 gatggaaaaa tgctgatgag gtagtaatcg taaacgactc atctatagca gatgctctat
      481 ctgcaacacc atttgcaaaa gcaaaaaatg ctccaatact tttaacaagt aaagataaat
      541 taaatgataa gacgaaagca gaaatacaaa gattaaaagc taaaaaagta tacttaatag
      601 gtggaacttc tgtattaagt acaaatatag aaaaagaaat aaaagattta aaaatatctt
      661 ttgagagaat ctctggagct gagagatatc aaacatcctt agaattagca aagaaattag
      721 atgctataag tgatgttaag aaaatagctg tagttaatgg tgaaaaagga cttgcagatg
      781 ctgtaagtgt aggagcacca gctgcacaaa acaatatgcc agtaatactt gctgattcta
      841 aaaatggaac agcagttgca gacaaattta taaaggatgc tggaataact caatcttatg
      901 tagtaggtgg agagagttca atttctgaag ctgttaaaaa taaactacca aatagtacaa
      961 gactaggtgg aacagataga aatgatacaa atgcaaaagt aataaaagaa ttttataaaa
     1021 agacagattt aaaaaatgct tatgtaacta aagatggtat gaataaacaa gaccaactta
     1081 tagatgcact agcagtaggt gtattaggtg ctaaaaatca atcaccagta gttttagttg
     1141 gaaagaactt atcagcttca caaaaatctc tagttaactc taagagtttt gataagataa
     1201 ctaaagttgg tgggaatggt aatgagacag catttaatga aatgaagtca ttacaagaag
     1261 taaaaacagt tgaagctaaa acaatttctg aattaaaatc agctatagat aaagctactg
     1321 ctaatgatgt tataaacttt aagccaacta gtgaggttaa agaagcattt acaatacaaa
     1381 ctgataaagc agtaacagta aacttaaatg gtacatatac aaagactgtg actatcaaca
     1441 tgcctaatgg tgatgtaaat aactatgcaa aagtggatga tgtagttata gatgatgtaa
     1501 aagatggaac ttttgttaat tatggaaaaa taactaactt aaaggttaat gataaaaatg
     1561 gagccaagat agaaaataat tctaaaggtg agattggtag tttaacagta gcatctggag
     1621 caagtcaagt aaaagtgact aatggtggta agataactac agttacaaat aattctaaag
     1681 ggactactat agataataaa ggtacaataa gtagtgttaa gggagataat tctccaacaa
     1741 taagtggaaa tagtccaagt tctaactctt caggaggaag ttcatcaagt ggaggttctt
     1801 cacatggagg aggctcatct tcaggaggaa gttcttctaa tcaaacatca gtaaataatg
     1861 aagcagcaaa aataacaagc gtgcctacac cagcaaaaga tgcaactaga ttaactatgc
     1921 catcagtatc aagtggatat actatagcaa taaaaacatc aagtaatgag agtgttataa
     1981 aaaaagatgg aacaataata cctccaaata cagcaacaac agtaaaatta gtatttacgg
     2041 taacacatac atcaagtggt aaaacagcag atacaaaaga attatctgta acagtaccag
     2101 ctaaatcaac agatgaagaa ttacaagcag cattagacaa tgaagtggca aaaataacaa
     2161 gcgttcctgc accagcaaaa gatgcaacta aattaactat gccatcagta tcaagtggat
     2221 atactatagc aataaaaaca tcaagcaata agagtgttat aaaggaagat ggaacaataa
     2281 tacctccaaa tacagaggaa acagtaacat tggtatttac agtaacacaa gaatcaagtg
     2341 gtaaaacagc agatacagga gaaatagatg tagtagtacc agccaaatca acagatgggg
     2401 aaatacaagc agaagtacaa gctgaggcgg ataaaataac aagtgtaact cagccaacac
     2461 aagatgcaac aacactaact atgccaacag tagcaagtgg atacacaata aagataaaat
     2521 catcaacaaa tgaaagtgtt atagcaaaag atggaacaat agtgccacca gatacagaaa
     2581 caaaagtaac attagtattt acagtgacac acacatcaag tggtaaaaca gcagataca
//
LOCUS       DAMJYX010000215         2609 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJYX010000215 DAMJYX010000000
VERSION     DAMJYX010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.220"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..857)
                     /locus_tag="PC479_003536"
     CDS             complement(<1..857)
                     /locus_tag="PC479_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2806048.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYP"
     gene            complement(897..1244)
                     /locus_tag="PC479_003537"
     CDS             complement(897..1244)
                     /locus_tag="PC479_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806049.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1462..2418)
                     /locus_tag="PC479_003538"
     CDS             complement(1462..2418)
                     /locus_tag="PC479_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2806050.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
BASE COUNT          891 a          375 c          300 g         1043 t
ORIGIN      
        1 ggatattctt caaattcatt gagttttatt aatttgaaag aagaagatag atttttaaag
       61 ttaataattt tagagttatt cactaataca cttctttttt taaaataaat ttctttttta
      121 cttatattta gtatagttaa tatttgttta agatatatta ttatatcttc tggagttcta
      181 tcaaatttga gttcaaaatt ttcatcttca aagtagggaa tataatataa aggtgttttg
      241 agagcaggta atttattata taattcttca agaatatcga tatttttagg ggagttaaag
      301 aaaaagcatt tacatacatt cattaaatcc acagcatctg aaagtgagtt atgtttaata
      361 tctccttcta cagaaaaaat ttttttcata ttcagtaaag atatttgctt agataagatt
      421 ctattattgt atgtaatatg ttgagatata aaaggttgaa tgtcaatcat catattaaat
      481 aactctaagc atttacctgt atatttatat actctaaatg aagttagaag gccagttata
      541 tcaaaatcac cccaattata tattgtaaca tcatcaaatt tacaaaacca tttataaaaa
      601 tcatccataa catataaaag attaggagca ttattgatat ctagttggtt caatttggtt
      661 aaatttttac atttatttga aagtatttta ttgtattttg gcttaactaa agagtaataa
      721 ttatctattg tattaaattc gttatcaact aaaactccac caatagaaat taattccgca
      781 ccattatatt cttttggttt atttttatta tcatctagta tattaaattc aaagtctata
      841 aataacttgt atttcataaa tctaaagcac atcctcaggt aaattattta ttgagcctaa
      901 gcatcataag aatcaataga tataatgact ctaaacatca aaggttcatt acaactttta
      961 tttatttcat aatttaattc aaataaatct tttaagatat aatttataag tattctttcc
     1021 caacccattt ttaaattaac tttagagtct attaatatat tttctatctt tttaaatatt
     1081 tctatatttg ctggtattat actgagctca atattacttt taaacgggtt agactgtaaa
     1141 tctgttataa gtatattttt taattcctct gtaagttcag aaattaaact atcacctatg
     1201 tattttttta tgatttcctc tctaatttct tttaaattta gcaatattag tacctgctac
     1261 ttacatagta gcttttcctt tccttttaat actattattc atttttgaat cttatttaga
     1321 tatattttaa gattaaaagt ctttagctat ataatttttt acccattttt aacttatatt
     1381 aacatattac aggcttatat ctccaccctg tttacattga ggatggagta tattgcctat
     1441 tttgataaaa atgataggtt gctatttgcc tattcctttt tctgataatt tttctaacat
     1501 atctttggtc attttatcta aatcataact aaacttaaag ttccattctt ttactgcact
     1561 actactgtct agagagttag gccaagaatc agctatactt tgtcttacag ggtctacatc
     1621 atattctatc acaaaatcag gaatatattt ttttatagaa tcagctattt cctcaggaga
     1681 aaagctcata gcagtaatat taaaagcatt tctatggatg agtttatcag ctggagcttc
     1741 gattaaatcc actattgatt gaagtgcatc aggcatatac atcatatcca tttttgtacc
     1801 ttctgctatg taagatttat atctcttatt ttttaatgcc tcataatata tatcaactgc
     1861 ataatctgta gttcctccac caggaggcgt aacataagaa ataagacctg gaaatctaac
     1921 acctctagtg tctactccaa attttgagtg ataataatca catagtaatt ctccagaaac
     1981 tttagtcaca ccatacatag tattaggtct ttgtaatgta tcttgaggag tcatattttt
     2041 tggagaattt tcaccaaaag cagcaattga actaggtgta aaaaatttac aatccaattc
     2101 tttggctatt tcaagaccat ttattaatcc attcatattt aagttccaag cttctagagg
     2161 tttactttca gcaactgcag aaagtaaaga tgctagatga attatagtat ctacatcata
     2221 tttttttgca atttcaaaaa aacgattctt atctgtaaca tctaatatct caaatatacc
     2281 agattcacat actggattac cttcttttac tctccttgaa ctagcgatta cattttgttc
     2341 tccatactca tttctaagct ttattgttaa ttctgaacct atttgtccta atgcacctgt
     2401 tataagtatt tttttcaatt ttacaacctc ctaagggttt aatttatgta gtactactta
     2461 tttttatttt agcaaatctt ttgtttaaat acaaagaatt aaagataata atatacgact
     2521 taattgaatg aagtgcagta agtgcctaaa aatttaaagc taatatataa tggtatattg
     2581 tataacaata aaagagtaga ttaaattat
//
LOCUS       DAMJYX010000216         2591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJYX010000216 DAMJYX010000000
VERSION     DAMJYX010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.221"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            159..758
                     /locus_tag="PC479_003539"
     CDS             159..758
                     /locus_tag="PC479_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806051.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(928..1395)
                     /locus_tag="PC479_003540"
     CDS             complement(928..1395)
                     /locus_tag="PC479_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806052.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            1506..2450
                     /locus_tag="PC479_003541"
     CDS             1506..2450
                     /locus_tag="PC479_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2806053.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
BASE COUNT         1000 a          286 c          396 g          909 t
ORIGIN      
        1 tttttttatt taattattta ttcattatga tttccaaaaa aataaattat aacttgttta
       61 attttagtaa ataaaatata aaaatataga aatactaaat taagaaagaa aattattaaa
      121 attttttata gtataaaaca tctaggaggg taataaaaat gaaattaaaa agaactttat
      181 ttgtaggatt agcactagta ttagctatag gtataactgc atgtactaaa aataatgaaa
      241 aaccaagtga aaatacagga aaaaatccta atgaatctgt aaataataat gaaggatata
      301 tgaattatta ttcaacaagc tataatgatt atatagcagg tctaaataga tattctattt
      361 atgatactcc agaaagtata aataatcagt ttaaagataa agaatatcca ggaaatgaaa
      421 aatatctaag tgatgtaaag gctgcatata aagatagtag agataaaata caatcatttg
      481 taacatctct taaaaaagat ggtaaaacag aagatacaga gttaaagaaa atgaatgatg
      541 acctaatcgc tgaaggggaa agattagtaa aagatataga taagagaata gaaaaattag
      601 ataaggtatc tgatgaagat atgaaaaaag gtcaaaatga ctttataaaa ttagttcatg
      661 gaactgaaga tgttggaaat gatataggta gtggttttag aaaaatgata aaagatatga
      721 atgatagatt aggaataaca ggaaataata agaaataata gaataaatag ttttgaaatt
      781 tgaaataaat tatttgagat atagcgaagc cttataaata atacattttt tggcttcgct
      841 tttttgcatg ttattaagat attaattttt atcttgtaac cttaatattt ttattataat
      901 ttatttagag gttattttca cacgttttta cttgtttttt ctctttttaa atttttgttt
      961 tgcaaaattg taagattctc ctattaaagt tttaaagttc tcaaatgtct tttcagtagg
     1021 gcttaggaca catatccatc ccatccatga gtagatagga tgtggcatga ttgtgtccaa
     1081 ttttgtaaaa tcaaaatcca tatcaactat ttgacctaca cctggacgct ttggaatata
     1141 tccaaacatt tcaataaaag tttctttttt taatcctata ttcacacggt atacatttgg
     1201 acgactaact aacgaacctt tatcattatt actgtctttt tccttaatgg taagaacata
     1261 tacccctcgc tttaaaacac cattaggatt gtagtaaatt gctcgttccc cccaactatc
     1321 cattagtaca acatcatcta agtttgatag acaataattg agaatatcat cagccttaat
     1381 agtatcattt tccataaagt atcaatccct tctttcttta ctattattat tgctttagat
     1441 gcaagaatat cttatcatat aatagtgaca attattgtca ctatatttaa attaggagta
     1501 taaatatgtc aagagctgag cgtttaattg aactgatgat aacaataaat gcaaaaagaa
     1561 gttttacagc aggagaatta gcagaagaat tttctgtatc aaaacgtact atactaagag
     1621 atttacaggt gctggagagt attggatttc cattatattc aaaagttgga gcggctggtg
     1681 gatatcatgt acttaaagag cgtatacttc caccgattgc attttcagaa agtgaggtaa
     1741 aatctatatt ctttgcatat cagtcactag aatattataa tgatttgcca tttgagcaag
     1801 aaactatttc tgttttaaaa aaatttttaa attgtatacc aaatgatatt caatataata
     1861 ttgagaatat aaggcgtaaa tttgtttttt ggacaccaga taggcactgt tcaacaccat
     1921 tattgaaaga attgtttaat atagtaatga atgaatttac aattaaaatt gaatattcat
     1981 caaagcaaaa aaatagtgta cgtaccattg ttcctattgg tttgtatgcc atgaatggac
     2041 tttggtattg tccagcttac tgtataaaat cagaatctat taaggaattt cgtgtagaca
     2101 gaattgtgaa gattttgagt atagaaaact tatcaagtaa gaaatataag gttttatctt
     2161 ctatacatga ttatcttaag aatatggaag taggtacaga ttatcatatt aaaattaatt
     2221 tgacagatga gggagtaaaa cgttgtgaaa cagaattttt gttggcaaga gggttgaaaa
     2281 tcttgtctaa aggtggatac atagatatgt atataccaaa atctacactt aactgggtgg
     2341 ctgaatattt tttaactttt gggaaaaatg ctacaataat agaacctata gaacttaaac
     2401 atttgataaa gtctaaagta ctagagctat ataatcatca ttgtatttaa attattttat
     2461 ttaatagtag gatagagttt ggataaaatg ttaattttct taatttactt tacagatgat
     2521 ttagatagat tgatgttatt taatttattt cttataatga tgttctatag ttatatagac
     2581 attaaaatat a
//
LOCUS       DAMJYX010000217         2571 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJYX010000217 DAMJYX010000000
VERSION     DAMJYX010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.222"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(216..1466)
                     /locus_tag="PC479_003542"
     CDS             complement(216..1466)
                     /locus_tag="PC479_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2806054.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1498..2439)
                     /locus_tag="PC479_003543"
     CDS             complement(1498..2439)
                     /locus_tag="PC479_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2806055.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          772 a          493 c          241 g         1065 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca aaatatttta
     1501 taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca catttactat
     1561 ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag gtgtaggaga
     1621 aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat aaacatctat
     1681 agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt catcaactaa
     1741 accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta tttctttttc
     1801 agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga aatctgcatt
     1861 ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg cctcacccat
     1921 tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa cttcttttgc
     1981 aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac ctttatactt
     2041 tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga atcttgcaag
     2101 aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat ttggagtatt
     2161 cataactttt atgccattag cttgagcata tttaacatct atattatcca gacctactcc
     2221 acctcttact atcaacttca actttttacc ctctaatgct ttatctatta catctttagt
     2281 aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat ctcctaaaac
     2341 atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt ttctcgctgc
     2401 ttctttctct attccatcag taactaatat attatacata acaaactcct ccaattttta
     2461 ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa aaactttact
     2521 taatttaact ttctgtttat ataattttca aaatattttt ttattaaagt c
//
LOCUS       DAMJYX010000218         2569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJYX010000218 DAMJYX010000000
VERSION     DAMJYX010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.223"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            296..2107
                     /locus_tag="PC479_003544"
     CDS             296..2107
                     /locus_tag="PC479_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2806056.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2195..>2569)
                     /locus_tag="PC479_003545"
     CDS             complement(2195..>2569)
                     /locus_tag="PC479_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806057.1"
                     /translation="TRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETLVDSYNF
                     PNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILYLLDIEA
                     VEIREQSKNIEEFLYKYKKRFK"
BASE COUNT          960 a          268 c          384 g          957 t
ORIGIN      
        1 agacattata caaatatatt attggggtga tattttgtaa gaaaagaagt gatgcagtaa
       61 attaattgag taatttaaaa atgatttaat gataatgttg atgaatgttt aaaaagaaat
      121 tttagaaaaa cgtatattgc tgtttgtgtt ggaggtgatg aatttgtaga atttataggg
      181 tataaatgat taaactattt aagataaata catatcactg taaaatattg ataatcattt
      241 tttataagag attgattatt tgcaaattat atatattaca aatgaggtaa caataatgaa
      301 gaaaaaatat gattttttaa tttattctgc tatttggcta gttattatta ttctttttat
      361 attttctcta tatcatagta tagagcatat taaaattata agtcacactg gtactattcg
      421 cagtgaaact caaaaggttg ttaagcaaga attgaataat gaaagaaatg atgacttgat
      481 aaaacgactg gataatattc tgataaagct aagaacaggt aatggagaaa atggatttca
      541 aagatgtgat aataaagaat ttcaacaaaa actaaatcaa atggattcta tgtgggaatc
      601 tatgaagaaa gaaattataa aagttagaaa tggtgcttca ggtgataaat tatataaatt
      661 gagtgaagag tattctgtgt tgtctaatca aattgttttt atatctgaaa aacactctaa
      721 tgcaaaattg tattcttttg ctactgcact atttatttat cttatattct caacaataag
      781 tcttttaatt tgggaatatt ataataaaaa aaggtttaaa agaatatttt atactgataa
      841 tttaacaaaa ataaaaaatc aggttgcatt tgaaaacaga gcgattgaaa ttttgcacaa
      901 tgcatctaac aaagagtatg tactgctaaa tattgatatt gacaatttta aatatattaa
      961 tgatacacat ggatatgaat atggagataa agttctaata atagttgcaa ctgttctttc
     1021 taaaactttt aatataaaag aaacttgtgc aagaatttgt tcagataatt ttgtaatact
     1081 tgcaaaatat agagattctc ttttagagga tattcgggaa atgctaacta atgctataat
     1141 ctctgaatta gatatgaatg taactcaaac tatatcttat tgtataggag cttacttagt
     1201 tgaaattgat aatttaggtt ataaatctat caattctatg atggataagg caaatattgc
     1261 acacaaggta agtaaaacaa gaggaatttc ctccacagta tggtataatg aaaatctgtt
     1321 aaaacagtta caaatggaaa atagtattta taattatatg tataaagcga tagaaaatga
     1381 agaatttcat atgtatttgc aacctaaatt tcaaatatct tctttaaatg ttgtgagcgc
     1441 agaagctctt gtaagatggt tttctccaga acttggtttt ttatcaccag atgaatttat
     1501 tccattgttt gaaaaaagtg gatttattat tgaattggac ttttatatgt taaaaaaagc
     1561 gtgttcattt gtaaaaaaaa cgtttatgaa aaaaaatcaa tatacatatc cgattgcagt
     1621 taatttttca agggttacta tttaccaaaa tagtttttat caaaggtttt tagatactgt
     1681 taaagaatat gaaattcctt ttaagtatat agagatagag gtaactgaaa gtgcttttaa
     1741 tgaaatatct caaccagtta tttcaatttt agaggaactt aaaaagttag gatttttaat
     1801 ttctatggat gattttggat caggatactc ttcattaagt cttttatgtt ctctatctat
     1861 taatggattg aagttagata aaagcttact taaagaaaca tttaaccggg agaaagtata
     1921 tagtattatt cagtgtatta ttgaaatgtc acatcgtata ggcatgtctg ttgtatgtga
     1981 aggtattgaa actaaaaaag acttggaatt tttaaataca gttaaatgtg atgtaggaca
     2041 agggttttat ttttctaaac cgatagaaga aaaagaattt tttaacaaat atgtaactaa
     2101 aaaataatat aattattttt atcagtctat acattgagtc attttatatc tcgttaaatg
     2161 actcaatgca ctgttatgta ttttaaaata taaattattt aaaacgtttt ttatacttat
     2221 atagaaattc ttctatattt tttgattgtt ctcttatttc aactgcttct atatctagta
     2281 aataaagtat agaatcattt gtaagttcaa catattcacc atcttctgaa ccgagtgcat
     2341 gtctctcaat ttttaatcct ttttcttcat aatatttttt tgtttctata tataatgatt
     2401 ctcgcttttg tatgtagtct ttcacttcat tatttggaaa gttatatgaa tccactaatg
     2461 tttcgccatt tataaaagtt ttctcggtag caactttttc atcccaacaa tggatttctt
     2521 cccaagtaaa aaatatctca gttccatctg gaatgaatga aaacctggt
//
LOCUS       DAMJYX010000219         2554 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJYX010000219 DAMJYX010000000
VERSION     DAMJYX010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.224"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            154..627
                     /locus_tag="PC479_003546"
     CDS             154..627
                     /locus_tag="PC479_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2806058.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(996..1553)
                     /locus_tag="PC479_003547"
     CDS             complement(996..1553)
                     /locus_tag="PC479_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2806059.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1811..2509
                     /locus_tag="PC479_003548"
     CDS             1811..2509
                     /locus_tag="PC479_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2806060.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          954 a          328 c          307 g          965 t
ORIGIN      
        1 atatataatt aaatacattc tatggtctaa ctacactata atataaatta tatattatca
       61 taatattcta aatttataaa acaaattgta ttgaattgaa tttatttaag atatataatt
      121 ataattaaaa taataataaa ggtgttgttt ataatgttca aagaatactt aataagagaa
      181 ttaaaagaag atgatattaa aaatgcatta gacttaatct ggaacgtatt tgaagaattt
      241 gaatctccag attattgtga tgaaggaatt acgacatttc atcaatttat agaatataac
      301 tctattattg aaaaaatcaa agaaaaaact ctcttctttt ggggttgttt tgactctaaa
      361 aaacttattg gagtcttagc tattagaaat ttaaatcata tatgtatgtt atttgtagat
      421 aaaaactatc ataaacatgg tgttgcaaaa aaattaatga ctacaggcat acaatactgt
      481 atttgtaata aatttaattt aactgaaata actgtaaatt catcacctta tgctgttgaa
      541 gtttatcata aattaggatt taaagatact gctgttgagc aaattgttga cggtatacgt
      601 tttacaccta tgaaattgtt aatataataa attaaataaa aagcaaagtc tcttaaatat
      661 tttatagtta ctttgctttt aaaccaagat ttaaaataat actatctttt ttgtttttta
      721 ataatatact ttaaaatcct tactaatatg aactataatt tattttttga tatccttttt
      781 gattaacata taaattaatt caattattaa acataaacta tgttgtttct ctttatatct
      841 cttctttgta aacaatattg ggcatttgaa aataaatatt cttaaaatat actgttctcc
      901 tgttgatttt cctttggtac aaatgataaa aatttaattt acatgcccca taatgaagtg
      961 ttcaataaat aagttaaaat aatttattta cctccttaat catatttttt atgaatacct
     1021 aataacacag tatcaataga atcggctaaa ccttttatag cttcttcttt actcaaaagt
     1081 gatttcccaa gttgcaaagt cgaataacca tgaagtacac tcattattaa gtttaatact
     1141 tcacctgtat tctttgtatt tagattacaa gaaagaataa gtttttctaa taaggattta
     1201 tattcatcaa agatttctaa tgtttcatta tttccatgcc aagtagccca ctgaattgtt
     1261 tcataaacac caggattaga aattataaaa ttaaagtatg ctatacttat acactttata
     1321 gctgtatctc caaaatttcc aatagtcgcc tttatcattt gactattcat ctctctcatt
     1381 cctttatgag ctacttcaag taataaatca tctagacttt ctatatgatt gtacaaagat
     1441 ggtgtacgta ttcctagctt ttctgcaata acttttaaag aaactttgtt aagcccatct
     1501 ttattaacta tatctgatgt agcttgtata actatttctc tactaacacg catgattttc
     1561 cctctttcat ttaaaattaa ttactttatt agtttagcta atctaattag tttcgtcaat
     1621 tattgtaatt tataaaattc tttatactat tataaaatct tgacagttta attataattt
     1681 aatataatac taattgaatt agttttaata ctaatataat tagtattata ttaaattcta
     1741 tattacaaca ttattttata gtttgattaa tttaacaatc actaatatat caaactgaaa
     1801 ggagattttt atggaaaaaa aatatttcga aattcatgga gttccttcaa tcctttgggg
     1861 aaatccttca agtaaattat atatttacat acatggacaa tgtggcaata aagaggaggc
     1921 tgaagctttt gctaatattg cagttccaca tgagtgtcaa gttttaagtg ttgatttgcc
     1981 tgaacatgga gaacgtaaaa gagaaaaaaa ttcatttaac ccatggcatg ttgttccaga
     2041 attagtatct attatggaat atgcaaagtg ccactggaat caaatttcgt tatatgcaaa
     2101 tagcattgga gcatggttta gtatgttaag ctttgaaaaa gagaacttaa gagagtgtat
     2161 ttttgtatct ccaattctag atatgcaaca gctcatttca aatatgatgc tttgggcaaa
     2221 tgtatcagaa gaacagctta aacatgaact aattatccca acttcatttg ggcatactct
     2281 ttcatgggaa tatctacaat atgtaaaaca acatcctatc actcaatgga atgtaccaac
     2341 aaaaatatta tatggtaaac atgatgaact tactgaaatc ggtgttgtag agaaatttgt
     2401 agaaaagttt aattgcaacc ttactattat agaaaatggc aaacattggt ttaacacacc
     2461 acaagaatta aattcattat atgaatggtt aaatacttta gttaaataaa aaataattat
     2521 attattaata tgattaaatt atttataaat gtta
//
LOCUS       DAMJYX010000220         2516 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJYX010000220 DAMJYX010000000
VERSION     DAMJYX010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.225"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..1123
                     /locus_tag="PC479_003549"
     CDS             <1..1123
                     /locus_tag="PC479_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806061.1"
                     /translation="NEDNLKIKTIKGDSNELDHININFGYALNGYINSYYTINGSNKT
                     KKTELIKLQKDSKNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDEKKEDLEKGVF
                     PSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDDYYAKILASKRYKKYLYVSVLYATSDS
                     SENNKEYSSTIDIIKINPENKKIKTIKSINLSNYFSEKYQNIENIGVSYASKYEDKMY
                     ILVNVLNTNIGDNSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKLEANKLYMVMS
                     NDYDNSSITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKIVAYTSRLNSI
                     LSWDDKFMLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
     gene            1132..1944
                     /locus_tag="PC479_003550"
     CDS             1132..1944
                     /locus_tag="PC479_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806062.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1955..>2516
                     /locus_tag="PC479_003551"
     CDS             1955..>2516
                     /locus_tag="PC479_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806063.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISG"
BASE COUNT         1081 a          200 c          380 g          855 t
ORIGIN      
        1 taatgaagat aatttaaaga taaaaactat aaaaggtgac tctaatgaat tagaccatat
       61 aaacataaat tttggatatg ctttgaatgg atatataaat tcatattata caataaatgg
      121 aagcaacaaa accaaaaaga ctgaattaat aaaactacaa aaggatagta agaacacaaa
      181 taatttaatt gatgtatcta gtggttcaga tttaattgct catgttggat ttgaaatgaa
      241 tgaggagtca aaagatataa actttgtgat agatgagaaa aaagaagatt tagaaaaagg
      301 tgtatttcca tcagaaggta gtgataaaga aattgaatat gaaaaattag atatgaataa
      361 ttttacaagg acaagatata aagtagataa aagtacagat gattattatg caaagatact
      421 agcatcaaaa agatataaaa aatacttata tgtatctgta ctttatgcta caagtgattc
      481 atcagaaaat aacaaagaat attcaagtac tatagatata ataaaaatta atccagaaaa
      541 taaaaaaatc aagactataa aaagtataaa tttaagtaat tatttttccg aaaaatacca
      601 aaacatagag aacataggtg taagttatgc tagcaaatat gaggataaga tgtatatttt
      661 agtaaatgta ctaaatacaa acataggtga taatagtaat tctaaatttc atttacttga
      721 gtataatctt gaagaaaata aggaaagcat aatggaatta aaaactgata gcaaaatggc
      781 tttagcaaaa gcaaagttag aagctaataa attatatatg gtaatgtcga atgattatga
      841 taatagttca attacagagt taaatttact tacctataat atattagata aaagcttaga
      901 tgaagataaa tatgttttag ataatagaga aaaaagagga attagaagta ttgaaattga
      961 ttcaaataaa atcgttgcat atacatcaag gctaaactct atattatctt gggatgataa
     1021 atttatgcta tatataataa ataaaaattc taaggaaatt gtatatgaag gagaattaca
     1081 atttaacagt tcaaatagta tgttagaatt tgaaataaag taggtgagtt gatgaaagat
     1141 ttaataagat tattcgaatt tgaattaaaa agaaatataa agaattatat atttataata
     1201 atttgttgtt gttcttttgt aattttaaac atagtaaaaa atttaaatga ctataattac
     1261 ataatagaaa atgcagttaa aagtgaacag ttaacaaaaa tcggaaatac gatagaaacg
     1321 gtaaatgtta caggtttctc aagctttaga aatataatgg gttctacaga atcgtggttt
     1381 atgtttggta taattgcatg tatttgctat gcttttttta tttggtatag agactttaat
     1441 ggtagaagta aatctatata tacacttata atgctaccta aaaatagaat caatatttat
     1501 atatctaaat tattaaatat attatttcta gtgtatagct atacagtagt acttacaata
     1561 tctttgttta ttgcaagtaa gttgttgcct agacatatgc ttggtaatgt cacaaactat
     1621 ggttttgtac aagaaacaat atatgagtta aaaatgttac ttccatatag ctttgaaata
     1681 ctatttgtag agtatatatt tttattgata ggatttgtat ctgtagtatt tacatcaatt
     1741 cttataaata aatctattta taaagtaagc actttaataa gttttctatt tttagttata
     1801 gaaattttag tgtttataat ttctatagaa tttattatac cttacgaata cagtgatgta
     1861 tttactgttc tatattcaag tataaacata tttgtatgta gtcttatatc taataaactt
     1921 ttgaagcaaa agattgactt ttaggaggga tattatgaaa gcaaaaaata taggaaaata
     1981 tacaatatgt tttataatag tatgtttttt aatgattact atgaccttaa tattgagtta
     2041 ttttaataag gataaactat ctgtaaaaac tttaaaaggc aatgtaaaag atatagggag
     2101 agtaagtgta gtatatacac ctaatataag caaattttct tatgaggagg taattataag
     2161 taaaaatgga gtaagaaata atacaaaccc aaaatcaaga attagaaatg attatttaaa
     2221 tattgacaaa aaatacagtg actttgttaa tgataaagga aaagaattta tatataaaga
     2281 tgatttgaat ataggagaag cgtatacctc tgttaagtct gatttagtac aaggaatgaa
     2341 tgctttaaat gattgtataa aggtttcaaa taaaaattta aaaagtgggg tagaagaaaa
     2401 ttttgatatt aatttaaata agtctgattt taaagaaaat agttggaaaa ccttatttaa
     2461 caaaatatat aaaggtaatt tatatatggt aattagtgaa actaatattt caggtt
//
LOCUS       DAMJYX010000221         2442 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJYX010000221 DAMJYX010000000
VERSION     DAMJYX010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.226"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            49..783
                     /locus_tag="PC479_003552"
     CDS             49..783
                     /locus_tag="PC479_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2806064.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(926..1417)
                     /locus_tag="PC479_003553"
     CDS             complement(926..1417)
                     /locus_tag="PC479_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2806065.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1766..2149
                     /locus_tag="PC479_003554"
     CDS             1766..2149
                     /locus_tag="PC479_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806066.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
BASE COUNT          949 a          276 c          361 g          856 t
ORIGIN      
        1 tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat gaaacaaaat
       61 aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga
      121 ttagcaggtg ctggagagtg gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa
      181 agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga
      241 gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa
      301 acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa ttttccaaaa
      361 gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc atttgaagat
      421 atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt ttctgttgaa
      481 catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga tggcactaat
      541 ctacactggc cagtagatgg atattttgaa gaaggagtac gtaagtcaaa ttttctagga
      601 gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt aatgaaaaca
      661 ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt
      721 cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc aaaaaaaaga
      781 taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa ttacactaat
      841 ggagatgatt ataccagtgg tattaattac accagtagta aggaggcaaa agcattcgtt
      901 gcttttgcca ttttttaagt tgcaattata cttctacgta gtatatttcg tttccatttt
      961 cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta ttatcatttg
     1021 gatgtatacc tattttatcc atttttaata aatctttatc aaatacaact tttacaacag
     1081 cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt
     1141 tttgaagtct ttcttccgat gcaaaactaa gaggtttact atcaatttgt tgacgaatat
     1201 cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct tttcctttag
     1261 catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt aattcttcca
     1321 tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg cttaactcat
     1381 ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat tagaaattta
     1441 ttttatatat aaataatatt atcacagaaa actttaaaag aaaacgtttt atataatata
     1501 gacaatagat tatattttta taaagaagca agaatactca ataaaactta aagaaattaa
     1561 acaatttcta aaaatacctt gaaaatagaa ccttatttgc catttataaa ttatataaaa
     1621 agggattata taaagtcata tatgaaaaaa ctatattata ctatcttttc tttttggaat
     1681 aaactatact ttaaatatag atatttaatt taaataaatt ttacagtatt aatagtaaat
     1741 tatcttagaa aggaatgtgc tttatatgtc tacagacaaa gttgttggaa tcatagatga
     1801 agaaacggca gaacttgcag gaattgaata tactggtaaa atttatgctt cttctggtgt
     1861 tataaaacat ataaagaaaa aacatagatg tcaactgagt aaggatattt ttaatgatat
     1921 aatagacact ataaaaatgg tattaaagtc acctgaatat ataggaagtc atcctaaaaa
     1981 acctggaaaa agtgtggagt ttataaaaaa aattgatgat tatattttag tagctacaga
     2041 attagataca aaaaaaggat atttatatgt atcttcaatg tatgctataa aagaagcaaa
     2101 attagaaagt agagtaaaca gtggaagagt aaaacaatat aatggttaac ataatattat
     2161 taattttccc taaaatattg aaaaatatta ttatataagt tataatatta atatacacgt
     2221 agatatttat tagttagatt tgaggttgga aaaggcaccc aatacgctac tatagagtag
     2281 ttaatagaga tgcaggatac gccgccctgc caaatctaat taaattaaaa agagaggatt
     2341 aattcctctc ttattgtttt tttgagatac taatttaatc tataatagaa agattgaagg
     2401 tcgtttatac aaaaaaattt tcagttatat acattaaact aa
//
LOCUS       DAMJYX010000222         2340 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJYX010000222 DAMJYX010000000
VERSION     DAMJYX010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.227"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            321..2216
                     /locus_tag="PC479_003555"
     CDS             321..2216
                     /locus_tag="PC479_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2806067.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         1029 a          238 c          434 g          639 t
ORIGIN      
        1 attaatttag tgatatatat ttaagtaata agagtaaagc aaaaattagt gcatagtagt
       61 atataaaaat ggagtttgaa tacttttgtt agtatatgat tgtttagtag aaaatcacta
      121 taaaatagga tttctatctt tgagcataat tatagagagc taagcagggg gaaggggttc
      181 aagaagatat tattagtgtt tgtgttagta aattatgtaa acacataaaa tatattgaaa
      241 aataatatta tatataatat tatttatgtg tgggaaatac gaatataaaa aataaaaaat
      301 aatattcgga ggacataaat atgttaagca acaaaaagag aagtatggca atagtaatgg
      361 caggtgctac agttatgagt gcagcagcac caatattcgc agacaatact gtgacagaaa
      421 atgttgataa aaattacaca gtaagtgcga aagattctgc taaactaatc gaagaggtta
      481 gaaaagcatt agaagttaag tttgaagata caaaagcagg agcaaatgta aatgatagag
      541 tttacgatat aaaagtagac aatgtaaact taactaatgc aactcaatta caaaataaga
      601 taaactcttt aacagaaggg caaagtttaa aagttactat tcaagataaa ggacatcaag
      661 tgttaggtgg aaaagtagtt gactataaaa ttgaaaacta caaaactgct caagaaatag
      721 tagatgcagt taatgcttac aatgcaactt tagcagaaga ttctgataac aagttaacag
      781 caactataaa atctacaaat acagttgaag taaaaagagc aaaagattca gcaaatgtta
      841 ttacattaaa tgtaggagac caacatttag atttctcaaa agtgataaca tcagaagaag
      901 gtacttttga aggttatgaa aagagttata gtgacataga ttcaaaagaa ttacatacga
      961 taacagttaa aaacgcagat ttacaagaca tatcagcaga agaattattt gatggaatta
     1021 gattaactac attaggaaga gaaatagtta ataaagttaa aaatggatat gctttaacat
     1081 ttgaaaatga agcaatcctt actcaagaac aagaagatag tgatgataaa gataaaccag
     1141 aaaaatcaag ctttgatata gttttaagca aggcaaatga aaagccagaa acaataagtg
     1201 tttcaagtaa aaaccataag ttagttagag acttacacaa agtattaact gatgtaaaag
     1261 gtggaaaaga attaaaagta gaagttttat ctggtgattc aagatttaca acagcagtag
     1321 aagttagtaa agagagattc aaagatggag aagcagaagc tataatttta gttggagaag
     1381 atgctatagt tgatggatta gcatcagcac cacttgcctc tcaaaaaaat gcaccaatat
     1441 tattatctaa aaaagattca ctaccatcag aaatagaagc tgaaatatta agagtacttg
     1501 gaagtaatct atcttctaag aaaatatata tagtaggtgg agaatctaaa gtatcaaaag
     1561 aaactgaaga aaaactttct aaattaggtg taagtaaagt tgagagagtt tctggagaag
     1621 atagatttga aacttcttta gaaatagcaa aacaattaaa agatacattt aagactgctt
     1681 ttgtagtagg tggaaatgga gaagctgatg ctatgagtat atcagctaga gctgctcaat
     1741 ttggtgctcc aataatagtt acaggtaacg aattagatgc aaatgctgaa aaattattaa
     1801 aaggaaaaga attagaaata gtgggtggag aaaattctgt atcaaaagaa gttgaagaca
     1861 aattagttga tatagattta aataataaag ttgaaagatt agctggagaa aatagaaaag
     1921 atactaatgc taaagtaatc aataaatact atgcaggtgc aactaaagca tatgtagcaa
     1981 aagatggtta tgtaggtgga aatggacaat tagttgatgc acttacagca gcaccacttg
     2041 cagctagttc aaaggctcca atagtattaa ctacagaaga actttctaaa tcacaagaag
     2101 aagtagttga gttaagactt aagaatgcta ctaagttagt acaaataggt gaaggaatag
     2161 ctaaaaacgc tattgaaaaa atagcagaaa aaataaactt atttactaag aactaataag
     2221 atataggaat taatattaaa agtttgttta gataatatat gattatacaa tgcgttaata
     2281 tgtaagacat agtgtagata ttataaaata tacaaataac aagataaaat aataaaaata
//
LOCUS       DAMJYX010000223         2333 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJYX010000223 DAMJYX010000000
VERSION     DAMJYX010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.228"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            66..512
                     /locus_tag="PC479_003556"
     CDS             66..512
                     /locus_tag="PC479_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2806068.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            514..1608
                     /locus_tag="PC479_003557"
     CDS             514..1608
                     /locus_tag="PC479_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2806069.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1696..2226
                     /locus_tag="PC479_003558"
     CDS             1696..2226
                     /locus_tag="PC479_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2806070.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
BASE COUNT          973 a          228 c          413 g          719 t
ORIGIN      
        1 taattgttta atataatata gttgatatag tcaaacatat tataattata gaagggaaat
       61 ttaagatgga ttttaaaaat ttacagtatg aaagtttgtc atatattatt tgtaacttgc
      121 aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc atataaactt acgaatggtc
      181 tatatttcta tttaatttat attaataaaa ataggaattg ttctttgaat gatgtttcta
      241 cagaatttga agttgataaa gctcatacaa caagaactat atctaggtta gaacaagatg
      301 gatacataga aaaaatccaa aaccctaatg atagtagagc ttttcagttg agagtaacag
      361 ataagggtga ggaagtatta ggggatataa aaaacatatt ttcaaagtgg gataatcaca
      421 taaaaaaaga gttctcggat acagaatata aagaactggt aaagaattta catgtggtaa
      481 aggatataaa aacagctgtg gaggaagaat aggatgtatt gtaatttatt aaaaccaatt
      541 aaaattggaa atttggaatt aaaaaataga gtatcttttg cacctacaag catgggtctt
      601 aaattggaag aaaaaattaa aaagttttct gatattgcta agtcaggagt agctctgatt
      661 actctaggag atgtatctat tagacctagt tttcataagg tagctataag cctatcggat
      721 gaagatggag tcatgaaata caagaaaatt gtagatgaaa tacacaattc tggtgcaaag
      781 gtatcagcac aactattttg ttcagattat gatgttaatt taattaaaga tactatgaat
      841 atgggaatta cttcacatga tgagataaaa aaaataatga atgacggagt aaaagattac
      901 ataacaaata tgcccaaaga agaaattaaa aatattataa atttatttaa agtaacagca
      961 ttgaatgcaa aaaaagcagg ttttgacatg attcaaattc atggtgatag gcttgtagga
     1021 agtttttcat caagtatatt taataataga aatgatgaat atggtagaac gtgtgataat
     1081 agaagtagat ttgcaagcga aataattagt agtatccgag atgaagttaa ggacattcca
     1141 atagactata aatttgcaat aagacaagaa aatcctcatt atggaaatgc aggagtttta
     1201 ctttcggaag ttgagtattt tgttaaaaaa tttgaatccc taggtgtaaa cagttttcat
     1261 gtaacacttg caaatcattc aaaattagaa gatactatac caaccaataa tcatccttac
     1321 tttaaagaag aaggctgttt tttatattta gctgatgaag taaagaaaca cacaaatctt
     1381 ccagtatgtg gggttggaaa attgtctagc ccagatttta tagaaagtat tatttcaaat
     1441 aatagagtag atatggtttc tatgtcaaga caattactag cagatagtaa ttggttacag
     1501 aaagtaaaag atggaagagt agatgagatt aaaaagtgct gttattgtaa taagaagtgt
     1561 gcagatgctt tacaaacacg ctctcagttt ggatgtatac tagattagaa tatccttata
     1621 aaaataaata atttgttaag gttaatatat ttttggtgat ttagataaat atttagataa
     1681 agaggaggag acatcatgaa aagtttagtt ttatattcaa gtttaactgg aaatactaaa
     1741 aagattgctt atactatata tgatgaaatt caagaagaaa aagacattaa agatgtaaat
     1801 gaattagttg attatggtat tgactatgaa aattatgata tagtattctt aggctattgg
     1861 gtggacaagg gaatttgtga taaaaattca aaacaagtgc ttgaaaatat tcacaataaa
     1921 aagatagcac tatttggaac aatgggagca agtaaaaaag gaagttatgg agctagtata
     1981 atagaaaaga tagaatcaat tatcccaaaa gataatgaaa tactaggttc attcatatgt
     2041 caaggtaaga tagcagaagg attgaaagca aaatataaag agatgttaaa actttctcct
     2101 gacaacgaac atattcgcca acaattaaat aaccatgagg aatctcaaag tcatccagat
     2161 gaacaagaaa tatatgaagc cagtatgttt gcaaaaaata tgatgattaa agctagtatt
     2221 gtgtagtagt taaacaataa gttcatataa gatatagaat aaataattta gtaaaaaagt
     2281 atagcaaata atttgctatg ctttttttat gaaatcaaaa aaataataaa aat
//
LOCUS       DAMJYX010000224         2281 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJYX010000224 DAMJYX010000000
VERSION     DAMJYX010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.229"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            65..1516
                     /locus_tag="PC479_003559"
     CDS             65..1516
                     /locus_tag="PC479_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2806071.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1735..2196)
                     /locus_tag="PC479_003560"
     CDS             complement(1735..2196)
                     /locus_tag="PC479_003560"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2806072.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
BASE COUNT          773 a          267 c          396 g          845 t
ORIGIN      
        1 attcattttt ttgattttaa gatttattga gaaaacaaaa ctaaataggg gggtaattaa
       61 tataatggat tttttcaatg gtattttaac gagtattaac aatgcagtag tatatgtaaa
      121 caattactta tggtcgtata tcttaattat aatgttagtt gtaattggta tttactttac
      181 tattaaaaca aattttgtac aattcagata ttttatagaa atgtttagac ttctgggtga
      241 tggtacagcg aataaaaatg caaaaaagga aggaaaaatc tcttcatttc aagcattttg
      301 tataagtaca tcttcaagag ttggaactgg aaatatagct ggaatagcaa ttgcagtggt
      361 agcaggagga cctggtgctg tattttggat gtggcttata gcattaattg gttctgcatc
      421 aagctttgtt gagagtacat tagctcagat ttacaaagtg aaagatggtc aagcttttag
      481 aggtggacca gcgtattata tggaacaagg tctaaataaa aaatggatgg gtattttatt
      541 ctcaatactt ataactatat gttatggttt tgtatttaat gcagttcaag caaatactgt
      601 ttcacttgca tttaataatg catttggaat aagtaaaatg gctatgggaa taggtcttgg
      661 aattttaact gcaatagtta tttttggtgg agtgcataga gttgcaaaag tttctgaaat
      721 aatagttcct gtatttgcag gattatatat attagttgct ttggctatag ttgtaatgaa
      781 tataacagag ataccaagtg ttatagcatt gatagttgaa agtgcatttg attttaaagg
      841 aatggcgatt ggtacattta tgggagttgt aatgactgga gtgaagagag gtctattttc
      901 taatgaagca ggtatgggta gtgctcctaa tgctgctgca acagcacatg taactcatcc
      961 tgtaaaacaa ggtttaattc aatctttagg tgtttttaca gatactataa taatatgtag
     1021 ttgtacagca ttcatggtat tattatattc aggttatagt agtagtggag caactggaat
     1081 tgaattagct caagaagctt taactcatca tataggacca gttggaaata tatttatagc
     1141 agtttgtata ttcttgtttg catttagttc tatagttggt aattactatt atggtgaatc
     1201 aaatatggag tttatgagtg gaagtaagac taaacttaat gtatttagag tttttgttgt
     1261 aggtatggtt ttatttggtt cacttactca agtggatgta gtttggaatc ttgcagactt
     1321 atttatggca ttgatggcga taataaactt aatagcaata gcacttttag gaaaatatgc
     1381 atttattgct ttaaaagatt atacaagtca aaaaaaatca ggaataaaag accctgtgtt
     1441 tattgcagat gaaatagaag gcctagaaaa ggtatcagag tggcataggg aattatctga
     1501 ggaacattta ggataaaaaa tagtataaaa ttataaaacc attttgaaag actagagtat
     1561 gtatatactt tatatgaata taatattgaa ttaatttata ttactggaat ttttaattcc
     1621 agtaatattt ttttattata taatttagct ttattaaaag tttttattat taaaaatagc
     1681 ttatttatcg taaagtgaat gatattgggt tttaattatt atgaatgtta gtatttaatt
     1741 taaagactta aataaatttg ccatttctag cgcattcata gcacaatcat aacctttatt
     1801 tccagctttt gtacctgctc tttcaatagc ttgttcaata ttttctgtag ttacgattcc
     1861 aaatataaca ggtaattcac tgtctaaaga tactttggct atccctttag agacttcact
     1921 acatacataa tcataatgag aagtagcccc tcttataaca cagcctaaac agattatagc
     1981 atcatactta ccacttttag ccattttttg agcaacaaga ggaatctcaa aagctccagg
     2041 tacccaaact atttctatat tttctggaga aacgtcatgt cttaaaagac agtcttcagc
     2101 tcctgataaa agttttgatg ttataaattc attaaaacga gaatttatta tacctatttt
     2161 taaatctttt ccaataagtt ttccttcata tatcattttt acttcctcca attttattta
     2221 tgttatagat ttatatttat aaatatcttt aatttaatta taaaaaacta cttagtagaa
     2281 a
//
LOCUS       DAMJYX010000225         2266 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJYX010000225 DAMJYX010000000
VERSION     DAMJYX010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.230"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            123..2186
                     /locus_tag="PC479_003561"
     CDS             123..2186
                     /locus_tag="PC479_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2806073.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
BASE COUNT          984 a          176 c          371 g          735 t
ORIGIN      
        1 aaacaggtat aaaaaggtat ataataaggc tttcatatac atttatgagt caaaattttg
       61 atataatata tagatacatg gagttatgaa tggaatttat ttaacttata gggggagtta
      121 atatgaaagc aggagaaatt gaatttctta gttatttaga ggggtcaaac aaaagttttg
      181 tagtacctgt atatcaaaga aattataact ggaagaaaga acaatgtaaa agattgtttg
      241 atgatttaga ggatataata gattctggat ttagaactca tttcttaggg tcagttgtat
      301 ctatatatag tatgggaaaa gaatatttaa taatagatgg acaacaaaga gtgactactg
      361 tgtcaatatt attgattgcc atgtgtcaaa taataaaaga gtttggggaa tcaaaaggaa
      421 ttattacaac agaagaacag attaaggagt gttatcttat aaacaagcac tcaagagata
      481 aagatggaaa aataaagtta aaaccaatag cagaagatag aaaagcgtac tatgaactat
      541 ttgaagaaga aatgaataca gatagaaatt ctaatatagt ttcaaattat ttatattttt
      601 ataatagaat aaaagaagat aatatatcaa tagatgattt gtttgatgct atacagaaat
      661 taactatagt agaaatagag ttaaaaaatg gtgaagatga ccctcaatta atctttgaaa
      721 gtctaaattc aactggctta gatttaagtg aagctgatag agtaagaaac tttgtgttaa
      781 tgggtagaag ctctaagtta caagaggaat tttataataa ttattggtat aaaattgaaa
      841 aaaatacaag tagcaacgta agtagtttta taagagacta tttgactata aaggagagaa
      901 gtataccgaa taaaaataaa gtatatttta gttttaaaag gtatgtatca gataatggta
      961 tagatataga aattttatta aaagatttac ttaaattttc taactattat agagagatat
     1021 cttattcatg tactgatgat aatgatgtaa acaaagtttt gaaaaggata aacaaacttg
     1081 aaatatttgt atcatatccg tttatattag aattatttga tgattataat gaaggtataa
     1141 taaataaaaa agtacttgta gaatccctta atttagtaga atgttttata ttaagaagaa
     1201 tagtatgtga tgtacctccg aatgcactga ataaagtatt tatgaactta ggaagggaaa
     1261 ttaaaaaatt tagtgattat aaagaaaact attctaatat attaagatat atttttataa
     1321 ataagaaatc atctcaaaga tttcctactg acagtgaatt ttcaaatgct tttgaaacta
     1381 aagatatata taatactaag tcaaagaata agttatatat acttgagagg ttagagaatt
     1441 atagtaataa agaaattgtt gatttagaaa atttaatcaa taataatatg ctaaatatag
     1501 agcatataat gccacagaca ttaacaaata aatggaagga atcactagga gataactata
     1561 aggagataca tgataaatat ttacatacaa ttggaaattt aactttgaca ggatataatt
     1621 caaaactaag taataaaaca tttgaagaaa agaaagaaat ggaagatgga tttaaagata
     1681 gtaggcttta tctaaataaa tatatttcat ctatagataa atggaatgaa gaagaaataa
     1741 aaaatagaac taagatatta ctagatagag ctgtagaaat ttggaaatat ccaagtgtta
     1801 catatgaacc tccaagtaac agtgaaaata tatttacgtt agaggatgat gatgttaatt
     1861 ttacaaatga aagtatagta tcatttagta tattaggaga gtcatataag gctaaaaatt
     1921 ggacagaatt ttatgaacaa gttgctctta ctttatatga tttggattct gtaaggttta
     1981 acagtattgt agacaaaact tataaaagag attttataga taatattaag tctagagatg
     2041 catataaatt aagaaaaggt attaaaatat cagaaaatat atatttagaa acaaatttaa
     2101 atacagaggc taaattaaat ataattagat ttttacttaa tgagtttgat atagagttaa
     2161 atgaagtaat attttccata aaataatgaa atatatgcat tttttgcgta atagaatatt
     2221 aattatatta aaaataaaat agcacctctt gatatagatt tatagc
//
LOCUS       DAMJYX010000226         2265 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJYX010000226 DAMJYX010000000
VERSION     DAMJYX010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.231"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(102..1016)
                     /locus_tag="PC479_003562"
     CDS             complement(102..1016)
                     /locus_tag="PC479_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2806074.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1843..2025)
                     /locus_tag="PC479_003563"
     CDS             complement(1843..2025)
                     /locus_tag="PC479_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806075.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          827 a          281 c          286 g          871 t
ORIGIN      
        1 aacttgttta aattttataa gtttataaaa aataaaggta cagttaactg tacctttatt
       61 ttatctaaat atgcaaagat tattttaaaa gtaattttta attacatgtt tttataactc
      121 tccaatattc taatataatg atttgcctct cttaaaacat ggtctgctaa aagtggtaat
      181 attatagatt ttatcttaca ctgttctatt cctgatgctc ctgcttcttt aaaatcttta
      241 aactcaacag tttcatttag agtttcttct gtaatgttct tgatattaga atcagtcatt
      301 tcgtttgttt tttcaattaa ttcattaaat tttatagcaa aatcatttga agtatttatt
      361 agttcacctt ctgaggggtc tagtaatcct ctcataaaca gagcatgttc catcataata
      421 tggtcccaga ataattctat ttctttagcg ttttttgact caatatctat tttattttca
      481 aggtcaacta cataagaacg atataaattt gcttcatgta ttatatgttc aagaagtaga
      541 gggtagtttg atgtaaatat agtacatgat agtacaccat ctaagactct ttctttaaaa
      601 ttaataagcc catcaagtaa tcttattgca tcagagttaa gttgagctac atagttcact
      661 aagtcttgac caacttgtgg gtttacacca ctttgtagat ttaattctct tgtagtgatg
      721 tttttgttta tttctatccc tgtaaactct tctgtttttt gttctgcaac tgatgtgaga
      781 gtagttacaa gctcttctga atataatata tcaggtctaa ttataccatt actagcacta
      841 acagtgtatg ataataaatc ttcaaattgc tttttatagt ggtcagcttc catagcaaga
      901 ttataatttt tatttgtgaa tcctgcttct aaaaaaagag catgctcttt cataattctt
      961 gaaaaaaata aatgtaattc tagtgataaa ataacatatt tttgattatc tatcataatt
     1021 tcctcccctt agaaagtatc ctatttataa tactttattc gtaatattta aatttatgta
     1081 tattataaat aaattaacta aattcaattg ctactaatat caacagtttt tattaagtta
     1141 tgggcgaaaa gattatctta ctgtgtttct tattagatat agatataatg atttaaggta
     1201 cttgttggct attatttatt aagagtacaa gtaatactag aaatttttat gagaattatg
     1261 taatttataa gtattatttt gatacaaaac aaatgttttt tgtaaaacaa gaacttttat
     1321 agcaaaaaat gaataaatat gaagcaagca taattttata ccacaacaat tattaattct
     1381 aatagctgca cttaatgttt taggtatggg atttaaaaag tataaaaaat taagtgataa
     1441 atatattcca ttatattgct tgtgcttgga gtagtatttt ctgtatagat gttaggattt
     1501 aatccaactt ctattttaca ggggattcta tgctggggtg tttcaattgg attaaattaa
     1561 gtatgcaaac aatttcaaga caatgataaa taattggatg tgtgatagga atatcaacaa
     1621 aaaacattat taaaaagaat tattttataa ataaaacaag taataagaat aaataaggag
     1681 caactaaaat gatttaaata tactagaatt aaaaagagaa tgtaaagttg tctagtaatg
     1741 gatgtaaaat attttaaacg ataagctatg aaattgatat attaaaatgg gtaagaagaa
     1801 tattatatag gattcttctt acccatttta ataaatttaa ttttatgcca aaccacattc
     1861 gcatagtaga atcatcactc tatggagaca taactctaca acatcttctc catatttttt
     1921 aataaaatca ccaatttgag tttctatgaa tgaatagtat tcttttaaac tagcaatata
     1981 tgcagtcatt atatgaacac attcatcaat cttatcttta aacacaacta atccccctaa
     2041 aaaacgaaaa ataaaaatat tcagtaaata gcagatatta tttcactcat aaatctatat
     2101 agtatgatat attgcttaga tatattttac aagtggatat gtaaaactgg ttattataat
     2161 cgcaaaactg gataaacttt cattcttaaa agaaaattta tccagtttaa agtttaatta
     2221 tgaatatttg agttagtaga tatttaatat aaattaatat aagtg
//
LOCUS       DAMJYX010000227         2257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJYX010000227 DAMJYX010000000
VERSION     DAMJYX010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.232"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(89..1381)
                     /gene="brnQ"
                     /locus_tag="PC479_003564"
     CDS             complement(89..1381)
                     /gene="brnQ"
                     /locus_tag="PC479_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2806076.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          749 a          373 c          247 g          888 t
ORIGIN      
        1 acaataattt aataattctt ttaaatataa aaaggaattg cctatataat ttaaaggcaa
       61 ttccataatt atcaattagt ttttattatt atgcagtttt actgctttta gatggaataa
      121 agtttcctat tattcctgct attacaactg gaactatcca gttaaatcct aatgaagcaa
      181 atggtaagct attaactaga ggaattttta caccaaaact tgttgcaact gttaatacac
      241 ttactaataa tgccatgtat gcagcacatt tgaacacgtt gtcattttta attttattac
      301 taaataatgc tagtataact agagttatag ttgctggata taccatactt agtataggag
      361 aagagaattg tattattgta cttactccaa agtttgatat gattgcacta aatacacaaa
      421 ctactataac tatatgctca tactttaatt ttccatctgt aagtgttgag aaatattgac
      481 cagctgctga agaaagacct atagctgttg ttaaacatgc taatgcaact ataatagcca
      541 ataaaatttt acctgtagtt ccaagtaaag ctgctgttat tgcaactatt aaagctgttt
      601 gttgaacatc ttgtccatat tgttgagaca cagttgctcc aagataacat aatccaccat
      661 aaactaatgc cattcctatt actgcaatta cacctgcact tattgtggct ttcatctttt
      721 ctttctcacc tgtataacct ttagctatta ctgacatgat tataattgat gcaaatacac
      781 tagctgccaa tgcatccatt gtttgataac cttgagttat accttctgca aatacatttt
      841 gtattaaaga actgtctctt atttctccta atggagatac gataccttta actattaata
      901 ctgctaaagc tataagtaaa gctggagtta atattgaccc aactatatct acaacttttg
      961 atggtcttat tgttaaaacc aatactagag caaagaatac tatagagaat attactgggc
     1021 ttattccaga acctattgta ggtaatatac ccatttcata agttgttgct gctgttctag
     1081 gtattgctaa aagtggccct atacagatta ccattgcaca tcctaatgca atacttaatc
     1141 ctttgcctgc ccttgagaac atcttcgatg tgtctccatt gaacttagtt gctgctataa
     1201 ctgctaataa tgccaaacca ccatctgcaa aaaggaatcc tatgaaagct actagccaac
     1261 tacttccaga taaaactcct agatatggag ggaagattaa gttccctgca ccaaaaaaca
     1321 ttgcaaatag tgcaaatcca attacaatta catctttaaa attgatttgt ttctcaccca
     1381 tttaaagcac ccccatttga attttactac cttttactaa aaaataagtc caatctctta
     1441 attaaatgaa aaccttttga cgagatttta tttaattata catttttgac ttaaatttgt
     1501 caattatgtt tcataattta taaaatcttc acaatattta atacaacttt cttaattaaa
     1561 caaaatcaac ctgtatactt taaacatagt tggttttttg ttttttctct aatttttctt
     1621 gataaaatta actattccga ataattaaaa tattaagact ttttagatta ttcgatttat
     1681 tattaagttg tattgcttta tatactgtat atatcactct ccttttttac attcatataa
     1741 tctttttaat tcaatttaaa ttttaaatat tttgtgttta ttatataatc tgaaaaaaaa
     1801 gcagtcaata gcatttttaa tttttttaat tttcaaaatt atgattctta aaaaatcgtt
     1861 ttccttttat cataacacct atttacgtta attcttgtag tattttgtat ttattttgct
     1921 aaattgacag ttaactttat tcaattttta ttctacttgc gtagatttgc ctcttgtttt
     1981 ttactcttaa aaccattttt tctgaaaatt ccttattatg atttatcaac ttatttttct
     2041 tcataacaat tactctctaa cttccaactt acttaaactt tgcagttgct agtattcacg
     2101 tttacatact tatatgtcat aattaactaa aaattcttaa tatatctctc aatgaaaaca
     2161 atttactcat ttatttaata catttatata atcatatgtc ttaaattttg ttttattatt
     2221 ttattattgt cacaatttta tttatattat atttttg
//
LOCUS       DAMJYX010000228         2229 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJYX010000228 DAMJYX010000000
VERSION     DAMJYX010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.233"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            59..376
                     /gene="trxA"
                     /locus_tag="PC479_003565"
     CDS             59..376
                     /gene="trxA"
                     /locus_tag="PC479_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2806077.1"
                     /translation="MVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(583..1857)
                     /locus_tag="PC479_003566"
     CDS             complement(583..1857)
                     /locus_tag="PC479_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2806078.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
BASE COUNT          784 a          297 c          321 g          827 t
ORIGIN      
        1 actgttatat aggtcgtagt atattaatat caagaagata ataaaggaga tgtatattat
       61 ggtaaaaata gtaaataata atgaatttat aaatgaagta gaaaataaag atggtttagt
      121 agttgtagat ttctttgcga cttggtgtgg accttgtaag atgctatcac caatatatga
      181 agctttagga aatgagatgg ttgaaaaagc aaacttcttg aaggtggaca tagaccaaag
      241 tatagaatta gctcagaaat ttgaagtatc tacagtacca actatgctaa tatttaagga
      301 tggaaaacct gtagatagat taataggttt tatgcctaag gagaatttaa aaaacaaaat
      361 tgaatcatat atgtaattaa agtaaattga agtaattaaa ataaatctca tatataaaca
      421 aaaaaactca ttgtaatgag ttttttgttt gtatagtgta aaatagtttg tatagttaca
      481 aatgagtgtc taattgctaa tgaaattgac tgttgatata atttgctttt ttgtgaaaac
      541 aaaactactc tctatttatt gataaaaaag aatagaaaaa acttatttaa aagccaaagt
      601 tttaatagca tgtataaagc tatccacctc atcaaaagta ttgtacattc ctaagcttat
      661 cctaacaaga cctacaggat tttccctcat aaatctgaca tatgcttctt ttggacttat
      721 acctaataat ttttgtacat aaatatctgc acctactact ccagctccag tttctattcc
      781 aaagttatca actaaatatt ttgctacatc tttgtaatac atattactta tattaaaaga
      841 tatatttggg ataattacat tatttgattt atctccatat attattatat taggtatttt
      901 actcatttct tctttagcat ataaaaataa ttctctttcg tattttgaaa ttctgtttag
      961 accaatttta tttaaagtgt tcagtgcttt tcccattgca agagttccaa aatactctgg
     1021 gtatcctgct tcaaaacgtt ggggtacgtt tgcatatagt attttgttat catttacaaa
     1081 tttggtagaa cctgagccat atagtacagg attaaatttt tcaaagaatt ttttaggtcc
     1141 taccagagct cctccattta gtggagaata acatttatga gcactaaaaa caacaaaatc
     1201 tatatgctca tcacattcat gtggcatcat tgaaaaaggt ttatgctgaa ttacttgcac
     1261 aatatccacc aatatctttg caccatactt atgagctatt cttgctattt catatatagg
     1321 agtggttata cctgtgacat tactagctcc tgtaactgta acgagagata cattaccagc
     1381 ataattttta agtatattct ctaattgaca taaatcaagt tctccttttt ctgtaattcc
     1441 aacaactttt gtaggaaaat taactttaaa aggcaggtaa ttagccatat gctccattgc
     1501 tgtggtaata acaaatttat ctggattttc atctttaaaa aattttgcta agaagtttat
     1561 tccttctgta gcatttttga cataaataac agaatccttt tcttcgtctc catcaataag
     1621 tttgaggata gtagagcgta cattttcata ttttaaagta tttttttctg agattattcc
     1681 tggagcatta atataagtaa gcaaaggaat ctcacagttt aagttatcca ctacgttttt
     1741 taaaactaat ggagtggcag agttgttaaa atatattctt ttaacagttt caccactttt
     1801 agttttaact ttaaggtttg caccttcaat gtatttagat acatccatga attgcataaa
     1861 aaaacccctt atctaataaa ctattctgtt ttttattaaa aaagctcaag ttttaatcaa
     1921 gagcttgttt aatatatgtt atgtttttat aaggggtttt gttaattaat tcttgttgag
     1981 atgtttatta aattacacga aaatgagcta cagttggggg aaactgtagc tcacttgatt
     2041 ttgggggaga taggttgaac ttattgttta tccgtatcat atttttagta tagtcactta
     2101 ttttaattta tatacatttt tttataaatt ttacataaaa tgttaaacac taattagaaa
     2161 taattatgaa tatgactaat aaagttgtaa aaatagtaaa gaaattgatt agaacataat
     2221 ggaaaatat
//
LOCUS       DAMJYX010000229         2102 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJYX010000229 DAMJYX010000000
VERSION     DAMJYX010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.234"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..913)
                     /locus_tag="PC479_003567"
     CDS             complement(<1..913)
                     /locus_tag="PC479_003567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2806079.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNIYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILS"
     gene            complement(907..1035)
                     /locus_tag="PC479_003568"
     CDS             complement(907..1035)
                     /locus_tag="PC479_003568"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806080.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(1174..1884)
                     /locus_tag="PC479_003569"
     CDS             complement(1174..1884)
                     /locus_tag="PC479_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2806081.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT          792 a          319 c          133 g          858 t
ORIGIN      
        1 cacttaaaat aacatctaga atcatattct gagtattaaa tatagtatta cattctttta
       61 tttgcttatt tatatcttta atatacttat ctgcaaactc atttttccca taaatattct
      121 gaatacaaat gatatgatta ttcatatcat gatatagttt tcttattttc aactgatatt
      181 cctgtaaatt taaataatat ttatattgca tattcattct tttttttgta atttcatatt
      241 ctatctttaa actattatct tttattattc ttcctattat gcttactaat gaaagatttg
      301 atagtaaaaa tacagttgat attgtaagta ttattaaact ctctttagaa tcgataatct
      361 tatctttaaa aataaattca aatattacta ttacaccaat aatgtttgct attaatggaa
      421 tagataaata tatacaatcc ctcttgttta tttttgtttt taatctgata aacttaagca
      481 atggtattaa tactattagt aatgatttag atatcattat taactctaac ttcaacaaat
      541 tatcctctaa taaatcattc atatttttta tagaattaag tgtcattact atacttaatc
      601 caatactatc aaatccaatt aataacatcc aatatagcaa acttataaat acacttttcc
      661 atttatccac tacataattt attttataca atacatatgt aataaatata catataaata
      721 atttaatatt tatattaaac tcaattgtat tcaacatagt aattatcata atagcaatta
      781 ctatgctact atttagggtt aatctattct ttcttaactc actaaattca tttaataaaa
      841 attttaatac aatccattct atcgccgtag aaaatatagg taaaaaaatc caaaatacat
      901 ttatatttaa cataatacat ctcccaatac atatgttaat tttttcttta aattagccat
      961 tctatattta ctaactggta tatctttacc attaataact acagtatttt tacatataaa
     1021 ttgaatatac tccaaattaa ttaaatagct cttgtaacat ttttaaaaat ttattctttc
     1081 ttaaatcact gaattcattt gataaaaatt ttaatacaat tcattctatc gccgtagaaa
     1141 atataggtaa aaaaatccaa aatacattta tatctaacat aatacatctc ccaatacata
     1201 tgttaatttt ttctttaaat cagccattct atatttacta actggtacat cttcatcatt
     1261 aataaccaca gtctttttac atataaattg aatatactcc aaattaatta aatagctctt
     1321 atgacatcta aaaaaattat atttttctag ctctaattct attttatcca tactattttt
     1381 agtataataa gtatcatttt tagtatgtat agtcaaaacc tttttcctaa cctctatata
     1441 cataatacta ttaattaaaa ttttattgat tataccttta ttttctatca gcataaaatt
     1501 ttctcttttt tttataatat ccgatataca actcaaaata ctttcattta aatctccaaa
     1561 atctataggc tttaataaat atctataagc ccttacctta tagccttcct gtatgtaatc
     1621 aactattgaa gttataaata ttatttcaga agtatcatta gtttctctaa ttattttagc
     1681 cacatccatg cctgttagtt gaagcatttt aatatctaaa aaaaatatat caataccttc
     1741 taaatttgtt tccaataatt cttctcctga attaaactca aatagttcaa tttgactaga
     1801 aataccctct aaggacttta atatacactc tcttaaaaaa gctatttgtg taatatcatc
     1861 ctcacagata actattctat acataaacac ccctccttag acattagatc tgaaaacaaa
     1921 taaaatatca atttataaat aattatttag ttatacaact aacatggtca tcaaatacta
     1981 ttattcactt aaaaaatcac cccctatttt tagtatgtta acaacaaaat aagaagatat
     2041 cttagatttt taaaaacaat taatacttaa tgtagttttg tatactattt ataggtaatt
     2101 tt
//
LOCUS       DAMJYX010000230         2065 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJYX010000230 DAMJYX010000000
VERSION     DAMJYX010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.235"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            162..1781
                     /locus_tag="PC479_003570"
     CDS             162..1781
                     /locus_tag="PC479_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2806082.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
BASE COUNT          758 a          257 c          313 g          737 t
ORIGIN      
        1 acaacttaaa aatttaacat tctcttaacg tatacttaac aaaacattaa tatttttatt
       61 gacaaaattt gttattctat attatggaaa caataaattt tagttaataa agtttataat
      121 tcagataaaa aaattattaa tttcttaagg gaggaaatat cgtgaatata gtaattagcc
      181 ttatcggtgg acttggactt tttctttacg gaatgagttt aatgggagaa ggtttacaaa
      241 aatcagcagg agacaaatta aaaaaaatta ttgaactctt aacaagtaat gttgttatgg
      301 gtgtactggt aggtacagtc gttacaggta ttatacaaag ttcaagtgct actactgtta
      361 tggttgtagg atttgtaaat gcaggtataa tgaatctaag tcaagctatt ggtgtaataa
      421 tgggggcaaa tataggtact acagttactg ctcaattagt ttcatttaat cttgaaggaa
      481 ttgcaccaat tgctttaggt ataggtattt tattctattt atttacatca aatcaaaaaa
      541 caaagcattt atctgagata cttataggtt tcggtatatt atttacagga atggagttta
      601 tgaaagatgc agtagctcca cttgctgaat ataaagcatt cactgatgca cttttatatt
      661 ttagtaaaaa tcctgtatta ggtatattgg caggatttgc cataactggt attgttcaaa
      721 gttcaagtgc atctatgggt atgttaatag cactagcttc tcaaggaata cttcctctat
      781 catctgcttt acctattctt tatggagata atataggtac ttgtgttact tcacttttat
      841 caagtgttgg tgcaagtaga aatgctcgac gtgcagctgt aatgcattta agttttaatg
      901 taattggtac aataatattt atgcttgttt taaataaacc tatatcagcc atagttactc
      961 attttgaccc aactgataca gctagacaaa tagcaaatgc acatacttta tttaatctta
     1021 caaatgttat aatattatta ccattttcta aatacatagt taaattagca aatagactta
     1081 tacctattaa agaaactgaa tcagaaatag taaataatac taagtactta gatgaaagga
     1141 tgtttagcac tccatctata gcacttggta atactgtaca agaagttgta agaatgggtc
     1201 ataaagctac aaactctttg gaacattcaa ttgcaggatt tttaaataag agcaatgaag
     1261 atattaataa agcttttgag tcagaaaaag ttgtcaataa gttacagaaa gatatattga
     1321 actatttgtt aaaactttct aaagaacctc ttagagatga tgaacgcttt agaacagatt
     1381 tgctttttaa tactgtaaat gacattgaaa gagtctctga ccatgcagaa aatatagctg
     1441 aattggccat gagtgttaag gaaatgaaca ttagtttttc tgatagtgcg ataagagaaa
     1501 tatacgaaat atacaataag acaattacta attttaagga tgccttaata gttttagatg
     1561 ttaaagattt tgagttagca aataaagttt tagaagttga aaatgaagta aattatttag
     1621 aaaaaacttt tagaaattca catatgatta gactaaataa tggaagttgt acaattgatg
     1681 ctggtgtact ttatcttgac ttacttacta atctagaaag aatttctgac cactcaacaa
     1741 atatagtaaa acaagtacta aaacttaaac aaaaaatata gtgattattt ttatatataa
     1801 tcttaatcaa tgagaagtaa gaagctatag ataatgctta tatcatttat ggcttcttat
     1861 tttttatgta atactcaaat ttttatagta ttctcttatt attaaacagt ttctgcctag
     1921 aatattccat atatatatga cttctttcat aaaacttgaa ctgcccataa ataaagtcac
     1981 aagtatacat aactattttt taatcaatta taaccaatat ttgttttatt tatcttcatt
     2041 ttaattcttc attcaataat gtaat
//
LOCUS       DAMJYX010000231         1939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJYX010000231 DAMJYX010000000
VERSION     DAMJYX010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.236"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(211..459)
                     /locus_tag="PC479_003571"
     CDS             complement(211..459)
                     /locus_tag="PC479_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2806083.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            complement(490..951)
                     /locus_tag="PC479_003572"
     CDS             complement(490..951)
                     /locus_tag="PC479_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2806084.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            complement(989..1822)
                     /locus_tag="PC479_003573"
     CDS             complement(989..1822)
                     /locus_tag="PC479_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806085.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
BASE COUNT          690 a          284 c          218 g          747 t
ORIGIN      
        1 acatttgata atgtccaatt catatatgca aaatttatat actttaaaat atctaagaaa
       61 ttattcaata aaaaaatact attttttaaa aattatcatt attgataaag ctctactatt
      121 tatatttttt agtgtattca tgtataataa gtaaaagcac aacggtatat actgttgtgc
      181 ttttttgtaa ttaataaaca atattttggt ctataacaat gaaaaatggc tactttccat
      241 aattgatttc atctgacata agcaaaaagg gtgtccagct gggtcaatca taactctcca
      301 gtcatcagta aattgctcct ctgcgattgt agctccacaa tgaattgcat attgaactgc
      361 ttcttctaaa tccttaacag cgaaatccat atgagccatt tgctgttgag attcaggttt
      421 ttctggttct attatgcccc aagcctttgt tttttgcaat ataaagaaaa aattatatat
      481 tttaatattt tatacttgtt gaaattgtgt aataaattca actggataca tagtgcgttt
      541 tgaaatttct tctaaagcta aaccttgttt taaaagcctc tttatgacgc tttccatact
      601 ttctgaaatt tctataatat ttttatcaaa atcataaaac ctaaaagttc tttgacccca
      661 tgggtattct tttatataat gcaaaaattc aatatttcct attctattta tcttactttc
      721 ccattgtttg atgtcattaa cctcaaaata caactgaaag ttatttgatt gttgatatat
      781 tggtaaatca tatccaatta tagctttata attactttgt aatccaaatc cattttcaaa
      841 tgatacatgt tctcctaaat ctaatgtgat tttttgctcc ataacatctt caaaaaactt
      901 ttttgattta tttatgtctt tgacaattag caatactcct ccaaatttca tcttatcact
      961 atcctttctt tgttataagt attataactt aaaattttgc aatttcactg taaaaatcag
     1021 acatattagc tcggtatttt tgaggagtta ttccacagac aattttaaaa tctttaataa
     1081 aatgtggtat atcatagaat cctaattcat tacatataga catccatgaa gttttattat
     1141 tgtttagaag ttttatagat ttattaatac gtaccaaacg agaaaatgac ttcatactca
     1201 accctagata ctgattaaag agtctgttta aatgcctaga actataaaat acttttttag
     1261 aaatttcttg tgaagatata tttccagagt tttgaataat caactgaatt gccaaaataa
     1321 actcatatga gtattcaatt ttaatattag aaatcaataa ttcttccaac tttaaaatca
     1381 atttatctat agataaagat tcattaaata tttttttaat ttctaaatct aatgaagtat
     1441 taattataga aaatggaaca actttatcaa taagttcact ttgctgtata cctataagcg
     1501 ggaaaaaacc agcaggttga aattctataa ttagtataac atcacatcta ttagcaatat
     1561 ttcctactgt ttttggttta gtagttggac caaataatag actatgtaac cctgtggcat
     1621 cataaaaaaa caccaaagtt acacttccat gaggaataat cgtgtaatta tcagaaatta
     1681 ttgtttgatt tggaaacgat atagtaaaat tcgatatcca atttcttaat tgtatacaag
     1741 gcaagagata aacaaaattg tcttcacaaa ctataagtga cctattttta tttataaaat
     1801 gaatactttc tttttttaac atatattata tcctgtccta tcgtgaatat ttattaaagt
     1861 aagaagaatc catattatct ctatatatca ttaactgctt ttaataatag agatagtatg
     1921 aatactcatt agatattta
//
LOCUS       DAMJYX010000232         1931 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJYX010000232 DAMJYX010000000
VERSION     DAMJYX010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.237"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            27..425
                     /locus_tag="PC479_003574"
     CDS             27..425
                     /locus_tag="PC479_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806086.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    861..1124
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1275..1826
                     /locus_tag="PC479_003575"
     CDS             1275..1826
                     /locus_tag="PC479_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2806087.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          812 a          185 c          293 g          641 t
ORIGIN      
        1 ttaaaaattt ttatgagggg gaaaatatga ataagaagaa aaaattgatt gggatagcta
       61 tatcttttct acttataatc agcattataa atccattaca agtagaagca gaagctatta
      121 ttgagtctag tgtatacatt tcttcaataa aagagaatta tacatatgat aatactaaat
      181 ttaaaactat taaatctaaa agtagacata aaagtaggaa atataataaa agtaaagtaa
      241 agttgaaaaa acgaagtata agaactaaaa agtatggata tcaaactata aataaatata
      301 aaagacaaaa cgcaagaaga cataatatcc taacaaaatt aatattgtcc ataatagtca
      361 tatttttggt tatagtttta gtagtgctaa tatttattaa aagaaagaag aaaaaatttt
      421 actaaaaata attaaaagaa tatcattttt ttaatcattg gtatctattt aataatatga
      481 tgtaattaaa atggagtaat attctgtttc aaaggaggca tataaataca ggaatgagta
      541 ttaaatgatg gttaattaag taaatttatt gataaatgta cttaattaac tttgaagaat
      601 ttaattcctc atgaatcaaa atataaaatt acatcaacag ttattcacaa aattaatata
      661 agaagaatta gagccaaaga gcagactatt agaccattaa agtaattatt catagaattt
      721 tgtatatgaa aagaaatgta tagtataatt tacttttttt ataaaaaata gattacaaaa
      781 tatatttttt ataaaaaggt attgacattt tttagaaaaa agaatatcat tattttaaca
      841 acaaaattca atacaataaa ttaaatccta tgacaaggaa gagtagttaa atttaggttc
      901 agtatagaga gctgaggatg gtggaatctt agcaagaaaa aatttgatga atggccctta
      961 gaggaataaa ttgaaatcat agttttgaga gtagacttta tcgtcagtcg cacgttatag
     1021 cgaataaggt atgtaagtac ctgataatga gatgtatgaa aattgaattt tatattaatt
     1081 tttatataat ttaggtggca acgcggataa tctccgtcct attagtttag gacggagttt
     1141 tttattttac tgaaaattta aaagatagaa ggtggtgaga aagatgttat gaatttcaaa
     1201 agctaaagat gatgatatag taggttggca aaaaaataaa ttgaaaatta aaaaaatagg
     1261 aggaagtaca aaaaatgaaa acaaatacaa aaaaattaac tttaaatgca atacttttag
     1321 cgatgggatt attaatacat caaatcacac cagctatagg attaccaatg aagccagatg
     1381 taccactagc aatgttattt gtaatattgg ttttaaatag agatgattat aaaacttgtt
     1441 tgatagcggg tatagtaaca ggaatattta cagcacttac atcaagtttt ccgggaggtc
     1501 aaattcctaa tgtaatagat aaaactctta cagcaaatat agtattttta ttaatgaata
     1561 ttagttataa tatgcctttt ataaaaaaat tagctaagaa aacacaagat actgtagtag
     1621 ttgcaataat aatgccaata ggaactcttg taagtggaac tatcttttta ttagcagctc
     1681 aagctatagt tggtcttcca ggggcttcat ttacagcttt attcttagca gtagtattac
     1741 cagctgtttt aataaatcta gttgcaggaa tatttttatt caagattgtt agtctatcaa
     1801 taagaagagt tagttatcaa gcttaaacac gaaataatat tttactttat aaagcattat
     1861 aataactaaa taacataaga ataaagtaga ttataactat tttattctta tgttgtttaa
     1921 agatttggta t
//
LOCUS       DAMJYX010000233         1925 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJYX010000233 DAMJYX010000000
VERSION     DAMJYX010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.238"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="PC479_003576"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="PC479_003576"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="PC479_003577"
     tRNA            242..316
                     /locus_tag="PC479_003577"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="PC479_003578"
     tRNA            321..395
                     /locus_tag="PC479_003578"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="PC479_003579"
     tRNA            401..476
                     /locus_tag="PC479_003579"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="PC479_003580"
     tRNA            482..558
                     /locus_tag="PC479_003580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="PC479_003581"
     tRNA            569..643
                     /locus_tag="PC479_003581"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="PC479_003582"
     tRNA            658..742
                     /locus_tag="PC479_003582"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="PC479_003583"
     tRNA            752..825
                     /locus_tag="PC479_003583"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="PC479_003584"
     tRNA            836..912
                     /locus_tag="PC479_003584"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="PC479_003585"
     tRNA            922..997
                     /locus_tag="PC479_003585"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="PC479_003586"
     tRNA            1009..1084
                     /locus_tag="PC479_003586"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="PC479_003587"
     tRNA            1087..1175
                     /locus_tag="PC479_003587"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="PC479_003588"
     tRNA            1193..1283
                     /locus_tag="PC479_003588"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="PC479_003589"
     tRNA            1292..1368
                     /locus_tag="PC479_003589"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="PC479_003590"
     tRNA            1454..1529
                     /locus_tag="PC479_003590"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="PC479_003591"
     tRNA            1590..1666
                     /locus_tag="PC479_003591"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="PC479_003592"
     tRNA            1673..1749
                     /locus_tag="PC479_003592"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="PC479_003593"
     tRNA            1753..1828
                     /locus_tag="PC479_003593"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="PC479_003594"
     tRNA            1836..1912
                     /locus_tag="PC479_003594"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAMJYX010000234         1879 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJYX010000234 DAMJYX010000000
VERSION     DAMJYX010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.239"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(65..493)
                     /gene="ytfJ"
                     /locus_tag="PC479_003595"
     CDS             complement(65..493)
                     /gene="ytfJ"
                     /locus_tag="PC479_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2806088.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            complement(447..1076)
                     /locus_tag="PC479_003596"
     CDS             complement(447..1076)
                     /locus_tag="PC479_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2806089.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            complement(1238..1777)
                     /gene="scpB"
                     /locus_tag="PC479_003597"
     CDS             complement(1238..1777)
                     /gene="scpB"
                     /locus_tag="PC479_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2806090.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT          594 a          299 c          154 g          832 t
ORIGIN      
        1 tgggatttat attaacccca ctactttact taaaactata tacatattta aaaatatttt
       61 attcttacat attgttttta tcttgattct tattactttg tgatatattt tgaattttct
      121 ccaatactct aggagttata gataatatat tatccactag attgatatta ctatctaaac
      181 tcattattct agtctctcca ctttcaacta ctacaaatgc tactggttgt acagatatag
      241 cccctgcgct acctccagca aaattcgtat ctgatttttc attttttaat tcaatttccc
      301 tatctttatc atcatatcct tttgagtatt ctcctcctcc tataccaaag ccaattgtaa
      361 cctttgatat aggtacaacc actgtagtat ctgtttttat agggtctcct ataatagtat
      421 ttgcatctat agaaccttta attgtttcaa gagttgtttc cataagactc tgtatcgaac
      481 ctgttgctct cattactatc accttcctta tttcctttat ttttattcat aattttatta
      541 atcattatta taatattaaa taaagctttt attcttggcc ttatatgtat ccttatattt
      601 cctaaaacat aatcttttgt gaaatctgga tttacattta aatacatttt ttctgcatca
      661 atcatattaa ataaatttcc atacaaagta tttatcaata catatacaga agatgttaag
      721 ccaatatttc cagttccaaa attaatattt gaataaagtt catgtatttt tatctttttt
      781 aataatctat aaatgctcaa aaggtcatca gctaataata tacttgaatc tatattattc
      841 ttcatcccct cttttttatc attattttta ctattttcag ctggataaag ttgtctattt
      901 atatttatta aattaaacat gtattttata ttaagcttta aacaaatatc tttgtttttt
      961 atatcagcta ttattaagat gtttatatga gatattaaaa tcaacatagt tacacttata
     1021 ataaaaatta cagctattat taaatactta aaattaaata attcattaag ctccatgttt
     1081 atattatcat caaaaaatta ctatttatac ttaattatcc atatttattt taagagaatt
     1141 ataattttat atttatacaa aaaaacacaa caacttagag cattttaaat cttttaaaat
     1201 ttaaaattac atactaaatt attgtgattt tatattttta ttcatttgtt gccacttctt
     1261 gataattttc tataggtggc aattcttcaa gactttctat attcaacaac tttaaaaatt
     1321 catctgttgt tttataaatt attggtttcc ctatcttatt caatctacca gcttctctta
     1381 taagttcatt ttcaaacaaa gtttgtaata ccttatcaca ttttactcct cttatatcct
     1441 ctatttctac ctttgttatt ggttgtttgt aggcaattat agtaagagtt tctaatgtag
     1501 cttggctcaa acttttcttt ttctttggtt caataatctt ctttatatat tctgcatagt
     1561 ctttatttgt acacatttgg tacttattct ctaacttgat tatttgaata cctctatttt
     1621 gttctctgta ctcctcaata agtaaattta acataatctc tatttctttt gatgaaagtt
     1681 ctttatttat tatatagttt aattctttta tacttattgg ttcaccatat gcaaacataa
     1741 cagattctat aatatacttt atatcttctc ttttcatttt aacactccga actttttctt
     1801 attagaatat catcaaaaaa tatatcttgt actacgacta tttctttttc ttttataagc
     1861 tctaatatag ataaaaaag
//
LOCUS       DAMJYX010000235         1849 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJYX010000235 DAMJYX010000000
VERSION     DAMJYX010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.240"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(104..319)
                     /locus_tag="PC479_003598"
     CDS             complement(104..319)
                     /locus_tag="PC479_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806091.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
     gene            complement(502..846)
                     /locus_tag="PC479_003599"
     CDS             complement(502..846)
                     /locus_tag="PC479_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806092.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            973..1356
                     /locus_tag="PC479_003600"
     CDS             973..1356
                     /locus_tag="PC479_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806093.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          691 a          235 c          230 g          693 t
ORIGIN      
        1 actataacat aggaattatc tttaaaaaca caaattaatt tacattctct aattttaaag
       61 tatatgtata tctttgattt taataaaatt cacttaaaat attttaaggt tgaacaatga
      121 aattgaatgc ccaagaactt cctcctctta ctaatgttgc aattaattca aacccacaat
      181 tgtattgaac tgaatctcta cctactggtt tccaactaga aggtgcatta tatgagaaag
      241 aatttgatgt tggctctttc ttttgttgct gcttaattcc tagctcatta gaaataaatc
      301 cagtttgatt tatatgcacc cttctatttg ttacacgaat ctggccgtct gcaatagacc
      361 agtttccaga aactttacta acggaaatat attgaaatgc tccatctcct tttgatattg
      421 tatatgtaac tgtaccagat gcttttacac caccagtttt atcccatcca tcttctgata
      481 tacttgcttt tctaagaggc tttattgtat tagtatcaaa aaaactgtct tagaatatat
      541 ctttgatgtt aaatttttag attgtttaac ttctaataat tgagttgttg atatagaatc
      601 aacgtatatg gactctcctg ttcttgcatt tttgattata cctttttcat tggaactttt
      661 aacatcagta attccatttt tagccatatc gtataatttg tccatgtctg ttacttgttt
      721 ctctgaatac attactttca tagatgaatc ttctacctta ctagcacttg catttgttgg
      781 taatagtatt gtcattatga gtaaagataa aatagttgtt acagattttt ttaacctata
      841 attcatatta atccttactt tttatttgtt ataacagaca ttctattgta atagaatata
      901 aatccacaat atttttaaaa ttagtattga ataactattc tcatgttata attattcaaa
      961 ggagggattg atatgaggaa aaaatttttt ataggtattc ttatagtttt aataatctct
     1021 gtgatagcta tacttaatat aaaaaatgat aaaaatgtct ttattggaga atcagaaaat
     1081 tggaaagcta aatttgtaac tactgaagga cactcaaaat tagaaataaa atataaaaat
     1141 agtaaagatg tagttgaaca aaaatttaat tattcctatc aatttataga tggtgaagat
     1201 gcagttgtag ttgtaaaaaa tgaaatttta gaagcaggtc aagaaaaagc aataaaaata
     1261 caaagaccag gtctttattc aaatagagaa tctagttaca agctaattat atcttggaat
     1321 ggaaatgagg aaactataaa cttgaaaaag aaataaccat attatcaatt tttaaaaaat
     1381 tattattcaa attcttataa aaagagtgtt tagaaagatg aaacactctt ttttgtataa
     1441 gtttttgaaa actataaatg aaatatatca aaatcgttcg aaaaattact gaaataaact
     1501 cttaaatata ttataaatta aggtatatta tacatgaatt tattgggagt ttatgagttt
     1561 taaaattgga aaccaaacaa taagggcgaa aaatacttca aggaaaatat ataaagttct
     1621 ataaattatc aaaaatattt cgcaaaaatt gataattatt acaaataaat tgataatatt
     1681 agaaatatcg aaattgaaca aactaggatt aaagctcaat tagaaaaatc cactaatcct
     1741 gaaaatactt tgttattctc atttattact gttgtttttt cattatttgc taatttattt
     1801 ttcagttcat taaatgttaa atataactct ataaacttaa ttttacagt
//
LOCUS       DAMJYX010000236         1834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJYX010000236 DAMJYX010000000
VERSION     DAMJYX010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.241"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(251..640)
                     /locus_tag="PC479_003601"
     CDS             complement(251..640)
                     /locus_tag="PC479_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2806094.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
     gene            1033..1389
                     /locus_tag="PC479_003602"
                     /pseudo
     CDS             1033..1389
                     /locus_tag="PC479_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1699..>1834
                     /locus_tag="PC479_003603"
     CDS             1699..>1834
                     /locus_tag="PC479_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806095.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     G"
BASE COUNT          653 a          228 c          198 g          755 t
ORIGIN      
        1 caactatttc ttctaaaatt atattccttt tataatttca atatctttat catatttaca
       61 tgatttaagc tattgtagta tttcatatat ctttaaattt taattggtat tattctctat
      121 attttttact atcaaacact aaggaaataa tcacttttct ttatataatg ttctaccatc
      181 acataaaact aacattaaat ccaaacgcta ctctcaattg tctcaaacta aaaagatact
      241 gatagcttta ttagtcatca atatcttctt ctttcaaatt tttaaagtat tcttctaatt
      301 tgtccaagct cttactatct aaaacttcat catcatgtag tgctgaaata aaactcttaa
      361 atgaattgtt atgcataaaa ctaaaaaaac tctttgtctc aacttttaag taatctttct
      421 cttttatagc tatagtataa tgcgtacatc tatctacctt atcagtagtt aaaaagccct
      481 tatctactaa attttttaaa aatgtcaatg ttgtgctctt tttccaatcg tatttcaact
      541 tcatgtcttc tatgatatct cttgaggcta atacatcatc tactccccaa atatacttca
      601 ttagttttag ttctgcatgt ggtaatttat taatcttcat tagtttacac ctcttaccaa
      661 tttatgtata actaaacttt ataacaataa gtgtatgtaa tcaattagat tggaataaat
      721 ataaattcca atttattcta tataaaaata attaataaaa aagaagtact ctactttaga
      781 gagtactttt ggattatata ttcaaaaatt tatctaacat gattagagta tgttttatgc
      841 tttagtgtga taattttagt tcgttttatt attgcaacat gtattatatt tcattataat
      901 ctataagctt aaatacagga tatttatttc atatacttaa tttatattaa gttttattat
      961 atgtattatt aagttttact atatttttta ttaatattta atatagttaa tttttaataa
     1021 ttatattata caataacaat atataagcta attaaaaacc ttaagaagaa cgatattgac
     1081 actacttgaa ttatattata gaaaagctga aataaataaa atagtttggg ctagaatagg
     1141 ttttaattta tatctttcta aaggagttgc attaacttta actgatgctg gtatagggat
     1201 ttcaggactt ttggctggtg ctattcctgg tataggacag tacttgccac ttatagttag
     1261 attaattaca atgtaccttg agtcaaatat aacatctgga aaaatcttta aatttaaaaa
     1321 atacgttata ataaaggagg tgcttgtgta accaaatact acttacaatc tgtagaaatc
     1381 aataggtgat aaaatgatta taaaatatct actttttgca attgtttcag cattaatttt
     1441 attattttgt aataaagcat tccaactaag taaattacac aagttcatta tattgtttgt
     1501 ttcatttttt atactaactt ttgtagcatc attcattagt agataaaaaa taagatggtg
     1561 gggttaactt accatctttt tctcatttaa tatttgtaat attttagata ctctttatac
     1621 ttgtgttact gttatacttt gattaggaaa attaatacaa catgagttga tgattatatt
     1681 ataaaggagg tatcttctat gaattttagg aacatttatc ttatacttat gggattggga
     1741 attataatat taactacaat aatatcttta attgaaatta aaattggttt ttgtaatgaa
     1801 aaatatttta ataaattaga atcaaaatat ggaa
//
LOCUS       DAMJYX010000237         1812 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJYX010000237 DAMJYX010000000
VERSION     DAMJYX010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.242"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            31..1170
                     /locus_tag="PC479_003604"
     CDS             31..1170
                     /locus_tag="PC479_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2806096.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1196..1384
                     /locus_tag="PC479_003605"
     CDS             1196..1384
                     /locus_tag="PC479_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806097.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          772 a          147 c          267 g          626 t
ORIGIN      
        1 gttaatatta atagataaag ataatctaag gtggtgatta aactgacaat agaagaatac
       61 aaaaacaaat actttactgg aaataaaatc gtactttcta taaatagttt tgataataaa
      121 gaactatact atcatgaaac tgaaaatata ttaaatttaa taaatgaaat agataaatta
      181 acatcaagta agatatttaa aacaatagaa gataaaaatg atttggcact agagtcttat
      241 tattctagtg taatagaagg tgctttttca actagaaaga ttgcaaaatc tataattaga
      301 ggaaagatga aaccaagtaa taaaagtgaa tatatgattt acaataatca tagagcctta
      361 gaatatggat tagataactt agatgaattg tatagtcaca aatttatata tgatttgcat
      421 catatattgg gcgaaaactg cttggattca gaagaatatg agtatagaac tgaaaaagta
      481 tatgtatgtg attcaaaagg tgagattata catacagggc ttgaaccttt gaagatatat
      541 gactttatta gtaaattaat agattttatg gaaaatagca aagtaagtaa tttaattaaa
      601 agtgctataa ttcattttta ctttgtatat gttcatcctt ttagtgatgg taatggaaga
      661 acatcaagag ccttatcata tctatatttg attgataaag gatatgatac tttcaaggat
      721 ttttcaatat catatatgat atctaaaaac agaactaagt attataaggc tatacttgat
      781 gtagaaaaca aaggaaataa tttaactgta tttatagaat ttatgctcaa gtctataata
      841 caatctatta atgagatgcg taatatgcat gatagaaaat ctttagaaag tatattaaaa
      901 gaagaattgt ttgaaaatga cataactcta tcagcaacag aagaacatat attaaaatat
      961 atttgcaata aagataacta ttctatgaca ttagaaaatt atataaaaaa gaataaaagt
     1021 agatacttaa aggctggaat aaaggaaatt gaattagttg accagttgat ggaagtgttt
     1081 aataatcttg aagagataga gattttgtct aaagaaaaaa atatatataa agttaatgat
     1141 aagtacttaa aaatgcttga tatagactaa tttagataat ctggagaggg aatatatgaa
     1201 taataagaaa aaggcatttt tgatttctgg ggctataaac gtaatttcat taattattgt
     1261 tttttcattg tttcattata atattataga aaatagtaca caaaatatga taataactgt
     1321 aatcttaatt tttatttttg aaatgataaa aataaaaatc atagagaaat actataaact
     1381 ataaacaaga aatatctttt ctaagagcat attgtacaat atgctcttaa tttagttata
     1441 tagatttaat ataaataatg tattttttgt agatgattta tgtaagattt atgaccaatt
     1501 tgtgtatgat ttgtgttctt taatcatgac aatattatat tgtaaaaaat aaatttaata
     1561 aaaaatgact tggaatttat attctaggtc ttttttatat aaaaatttaa taagtttcaa
     1621 atgttggtaa ctttgacagt agaaaacgtc tattgagata acgaggattc taattgaggt
     1681 ggagagatat tagcacttaa agtagtatat gcaacagata taatctttac aaaagtaatt
     1741 tgaattaaaa tgtaaatata aagaataatt aaaaaaatat cgcaattatt tgtttttcat
     1801 atggataaaa tt
//
LOCUS       DAMJYX010000238         1745 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJYX010000238 DAMJYX010000000
VERSION     DAMJYX010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.243"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            101..463
                     /locus_tag="PC479_003606"
     CDS             101..463
                     /locus_tag="PC479_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2806098.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            899..1600
                     /locus_tag="PC479_003607"
     CDS             899..1600
                     /locus_tag="PC479_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2806099.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
BASE COUNT          712 a          143 c          268 g          622 t
ORIGIN      
        1 attttttatg ttgaaagctt ataatgatta tgatataaat tggttatagt atacaattta
       61 tgggtataac aataaaaaat tagaaaggaa tttttataaa atgattaaat tatatggata
      121 tacaaaatgc tctactgtta aaaaagctaa aaattggctt aaggaaaata atttagaatt
      181 tgaagatata gatatggttc aaaaccctcc tagtaaagag gagttaaaat caatttataa
      241 gactagtgga tatgatataa agaaattttt taatacaagt ggtatgaaat atagagaatt
      301 gggtcttaaa gatattgtaa aaactgaatc tgatgataaa cttttagaaa tattggtaag
      361 tgatggtatg cttattaaaa gaccattact tttggatggt aaaaatgtat tattgggctt
      421 taaagaagat gtatggaaaa gtactttgct aaaagaagat taggaagaga taggtgtgtt
      481 gattaaaatt attaattaat acacctataa atttatgtat taaatctaat ttttataaat
      541 attaattagc aaattcaaat caaaaaaatt catatcaaca aacatattac ttcttattac
      601 aaattatatc atatgtttta tattaaaata ttataatatt acaacgaatt gttacaaaat
      661 tacttattat taaccatgaa catttgtttt gtgctatcat atttatataa gggaattgtc
      721 ataaaaaata tcttgttcat acagttttat atagaaaaaa gaaatgaatc ttttctttat
      781 ataagtatat tttgtaaatt gcctctatat ttatagttta aataatagtg gtattaaaaa
      841 tctgacaaca aaaataaaac tttcaaaaaa ttaaatctaa aaagaatagg agataaatat
      901 gagtattgaa gatttatatg attttgaatg tgttcctttg aacaatgaga ttgatattga
      961 tgttttattt gaaaagatga tagaagatgg aaaagaaaaa gaatatacac caattatagt
     1021 cattgatgaa aaagtagggc tattaagaaa aaatatagat atgattacta aagagtatgg
     1081 ttctcttgaa aactatagag agtattgttt aacaaaatat aatgaaattg atgtaaatca
     1141 attttttgat tttaggaaga gtgagatttc agaattatta aaggagctaa ttgatttaga
     1201 agatgatttt tatgattata aacattttag accaattatg aatatagata tatttcatga
     1261 atataacaaa gtttacatag ctaaaattcc aacaattaaa ccatatgaag tatttgcata
     1321 catacctatt ggcggattta atgattgtcc tagagatgaa gaacatatag ctattgcaaa
     1381 atattggtat gaaaagcatg gaatctttcc aatcgcaatt ggatgtgata cagttcaata
     1441 ttctgtaaaa aatttaacta aagatggtaa aaaatttgat gaattatgta ttgaattagt
     1501 attttattgt gaggatatta ttatacaagg gtatgaaacc ttaaaggctc ttaaggatgt
     1561 attaaaacgc tctacaatag tgcttttttg gtgggattga tattccatca tttagatagt
     1621 attaaattgt tagtcaccaa gaatccactt gctttagtta tggggagtgt gtcaaactag
     1681 gtttttatac acagtgtagc atagtaatat gtttaaatta caaaggttaa aaaaatactt
     1741 taata
//
LOCUS       DAMJYX010000239         1665 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJYX010000239 DAMJYX010000000
VERSION     DAMJYX010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.244"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            86..1414
                     /locus_tag="PC479_003608"
     CDS             86..1414
                     /locus_tag="PC479_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2806100.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          556 a          197 c          316 g          596 t
ORIGIN      
        1 aaagctattt ttctatatat tggcataata tatgcttatt taactatgaa attaaaatta
       61 aataggatta atgaggagga aaaaaatggg aagtaatgaa ctgaaaaaga ctcttggcgt
      121 atcagcagca ttatcaactg ttgttggttc cgttatagga gcgggcgtat tttttaaacc
      181 acaagcagtc tatacattaa ctggaggagc tccaggtctt ggtatattgg catggttaat
      241 agctggaatc ataactataa cagctggtct tacagctgca gaggtatcag ttgcgatacc
      301 aaaaactggt ggaatgatgg tatatataaa agaaatttat ggagaaaaat taggtttttt
      361 aactggatgg atgcaaattg tattatttta tccagggatg atggcagcat taggtgttat
      421 atttggagaa caagcatcgg cactaattgg tagtccatca cttttacttc caatagcaat
      481 tggaattata ataatagttg cagggcttaa tatgttaggt tcaaaaactg gaggagtcat
      541 acaaactgtt tctacaattt gtaagttaat accacttatt ttaataatgg ttgttggatt
      601 tattaaaggt ggtggaaata atccaatact tacacctatg gttggtgaag gattaagcct
      661 gggtagtgta ctaggtcaag ttttaattgc tatattattt gcttttgatg gatggatgaa
      721 tgttggtact ttagctggag aaatgaagaa tcctggaaaa gaccttccaa aagctataat
      781 aggtggattg tcagttgtta tggctgttta ttttataata aatttagcgt atctttgggt
      841 attaccagct aatgaattag caaattatgc ttctcctgca tcagcagttg ctgaagttat
      901 atttggttca atgggtggaa aaataatatc tgtaggtata ttaatttcag tgtttggagc
      961 attaaatgga tttttactaa ctggttcaag ggtggcatat actttagcta cagataagac
     1021 tttaccaaag tatggtatat tttctaaatt aaattctgct caagttccag caaatgcaat
     1081 agcattagta tctgttattg catctatata tgctttgtca ggtcaattta atttattaac
     1141 tgacttagct gtatttgcta cttggatatt ctatgtatta acttttatag gggttatgaa
     1201 acttagaaag actcatccaa atataccaag agagtataaa gttccattat atcctattgt
     1261 tcctataata gctatagcaa gtggtatatt tgttgtagtt aatcaattgt gttttgcagg
     1321 tatgaaaact actatgatat ctataggagg tcttgtaata acagctatag gcttaccagt
     1381 gtatgcttat atgactagag gagtaaaaag ataaaccaag tagtttacaa atgcttcata
     1441 attaaaataa aagagactgt ctcaaataga gataagtctc ttttttattg ttaaaatggt
     1501 aaagatatgt ttaattttac aaaaaaagag cgttccatga actaaaaagt ttattttaag
     1561 gtattcattt tatttttagt tttttgatta taaaattaaa catctaaaaa attatttatt
     1621 ttaaaatact ttattttaaa gtgtttttta tcaaactaag tggta
//
LOCUS       DAMJYX010000240         1541 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJYX010000240 DAMJYX010000000
VERSION     DAMJYX010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.245"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            102..524
                     /locus_tag="PC479_003609"
     CDS             102..524
                     /locus_tag="PC479_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2806101.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1052..1420
                     /locus_tag="PC479_003610"
     CDS             1052..1420
                     /locus_tag="PC479_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2806102.1"
                     /translation="MKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          665 a          151 c          190 g          535 t
ORIGIN      
        1 tatataaata gttttaacat gatttaatat ggcatattat actattttaa cttaaaaatt
       61 aacttataaa tattaaattt atatacagaa gggaaaacga tatgaataca tttaaaactg
      121 aagttttatt aaaatattct gcaaaagaca tatttaatat atttacaaaa tctgcaagac
      181 taaattttcc taattttaat gagaaaaaag cagtaggaac atttgtacag caaaaaaata
      241 ataaaagatt taaggttgaa ataactaatt tcgaaaaaaa tagagtatat gagataaaaa
      301 caagcaataa aagagaaagt actattacta gatatgaatt agtacatatt gacttagaaa
      361 atacaaaact tatttttatt gaaagtgaga gcaagaggaa tatatttgga aaaatcaatt
      421 cagctcttgt aagagtattg tttggaaaaa ggcaaccaga agaatttaat aaatttataa
      481 aaaaactaga aatggaattg gagaataata gtgttaattt atagatataa gttagattat
      541 atatttaaaa tctatctaaa aacatatttc taagtatttt atgagtccta ctattcgtta
      601 caataattaa tgaaaaaact attgataaac atgtgacact atgactatat accacattaa
      661 gagtatcatt agcaataata attttatgta ttattgcttt tagttaaaaa aagagagaaa
      721 aattaataat ctctaatatc ccttaaactt tcaataaaga ggataatctt tatctaataa
      781 gattctcctc ttctaattta gaaaagatat taatttattc actatatagt atttttgtaa
      841 aaatcaataa aaaatatatg tcatattctt atctctaata gaaaatatat tcccctcttc
      901 cataaaactc tcaatatctt ttttatctat aaatctatac tacttagttc tcaagcataa
      961 aataatataa agattattta tacatgatga tatattatat tgactattag acatttgtct
     1021 aatataatca agttaaaggt gagtgattat attgaaaaaa ataccaaaat ccgagttgtt
     1081 agttatgaat ttcatatggg ataaagacat agataaaata ccagccagag aagtagctaa
     1141 ttatatggaa atttctcatg gatggacaaa aggtactact ggtaaagtac tttctagatt
     1201 aagtgataaa ggttttttag aatttgaaaa agatggtaga aatactttat acactattaa
     1261 agttaaacat gaggaatatc taaaatttga aacaaaggat tttttagagc ttatacataa
     1321 aaattctatt catagcttta tatcatcatt aggttgtgga aatgaaatat cagatgatga
     1381 cataaaagaa cttgaacaat ggctaaaaaa aagaaaatag gagacgtatt ttatggaatt
     1441 atttatagaa gttattaaat ttaatattta cataagtcca ctaattcttt ttttcatttt
     1501 atttactaaa aagataagca aatatacttt taagtttaat t
//
LOCUS       DAMJYX010000241         1530 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJYX010000241 DAMJYX010000000
VERSION     DAMJYX010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.246"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(3..950)
                     /locus_tag="PC479_003611"
     CDS             complement(3..950)
                     /locus_tag="PC479_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2806103.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(919..1365)
                     /gene="lspA"
                     /locus_tag="PC479_003612"
     CDS             complement(919..1365)
                     /gene="lspA"
                     /locus_tag="PC479_003612"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2806104.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
BASE COUNT          518 a          276 c          148 g          588 t
ORIGIN      
        1 ggttatttta tctttttaat aacttcctta aaatactcag gtaattctga attaaattcg
       61 atatattcct tagtagtagg atgtataaat cccaacttct tagcgtgcaa agtttgtcct
      121 tttaatttaa atttagtttc ttttggacca tatacataat cacctaaaag tgggtgattt
      181 atagacaaag catgaactct tatttgatga gttctaccag tttcaagcct agctctcata
      241 tgtgtaaact tatcaaatct ttctacaact tcaaagtgag tgattgcatt ttttccatct
      301 tttactacag ccatttttaa tctatcctta ggatttctcc ctataggttt atcaacagtt
      361 attttatctt ctttaaccac accatagcat ataaattcat actccctagt tatagtatgt
      421 tcctttaact gttctgctag aaaattatgt gcataattgt tcttagctat cataagaaga
      481 ccagaagtat ctttgtctat tctatgaact ataccaggtc taataactcc atttattgat
      541 gacaacttat ctttacaatg atataaaatc ccatttacta acgtattttc atagtttcca
      601 ggagcaggat ggacaaccat atcctgaggt ttatttatta ttaagacatc atcatcttca
      661 tatacaatat ctattggaat atcttgaggt aaaacttcta atactttagg ttctggtatc
      721 tctataacaa tattatcttc ctctttaacc agatatctag ctttttctac tttattatta
      781 acctttactt ttccatcttt tataattttt tgtatataac ttcttgacat atcacctaat
      841 tgttctgcta gataaatatc aagtctaact tcttcttcct cttcaagtac taaaaactct
      901 tttacttcat ccattatatc acctactttt actttcaaaa aataaaacat atatacataa
      961 aaacacagtt cctacaacta caaatacatc agctacattg aatacatatt cccatataac
     1021 tctaaagtcg aagtaatcta ccacaaaacc taatcgtact ctatcaacta gatttcccaa
     1081 tgcaccagat ataattaata taattcccaa ccttccaaat atatgtactt tccttgtatt
     1141 aagatagtat aatccaaata ccgttgcaag taatgcaaca attataaata tccattgatt
     1201 attttgtaat aatccaaatg ctgcacctct attttcgaca taagttaaat gaaatacatt
     1261 atttattatt ggtattgttg atacatccac caaattattc aatacccata ttttagacag
     1321 ttggtctaaa cctatgagta gaattattat taatatatat agcaatgatt tttcccccta
     1381 taaattattt aaaaaccaat tttatttgaa gcacactatt attattatac aaaaaaaatg
     1441 ctctaaagag caactttatt aaaattgtat tttcttaata aagctagaac aaaatttaaa
     1501 atagtactac tttttttata ttgtagattt
//
LOCUS       DAMJYX010000242         1458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJYX010000242 DAMJYX010000000
VERSION     DAMJYX010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.247"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            102..383
                     /locus_tag="PC479_003613"
     CDS             102..383
                     /locus_tag="PC479_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806105.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            647..901
                     /locus_tag="PC479_003614"
     CDS             647..901
                     /locus_tag="PC479_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806106.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            946..1269
                     /locus_tag="PC479_003615"
     CDS             946..1269
                     /locus_tag="PC479_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806107.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          612 a          107 c          233 g          506 t
ORIGIN      
        1 tagaaaataa tagtgttact ttcataataa atacatataa agatattttt aagaataaaa
       61 tttattatta aataaaaaat aaaggagagt gatttaatag catggcatca aaacaaatac
      121 caagaaacaa tttagaaaag tttttaagaa taagagtctt atcacataca ggcgataaaa
      181 tacatataaa gttacctgta aattttgcaa agagaatgat tgagaataat gctttggatt
      241 tatttaatgg aaaagatgat gtagtagatg gtaaaaaagt tactgaaata ttagttaagg
      301 catttgacta tgatttaact ggagaagtag ttcacttgga gagaaaaaat ggagacatta
      361 ttaaagtttc aattgtacaa tagaattatt ttatatgaat tttatttttg aatagttata
      421 aataattaga aatttgtaaa aaagtataat atattattag taatggtaca gaatacacat
      481 ttataattta tagatgtgta ttttttgttt aaaaaattta aatatataaa actataaagg
      541 ttacaatttt ggaaatagat gtaaatatat gttgtattgt ataataagag taatataaaa
      601 tctagtaaaa ataatcaaat aaatttagct tatgagaggg ataactatgg aaaataagga
      661 ttttaaatta acaatatgta tatttgcttt tgtaattgga gtagtagtat tattctgttg
      721 tataggttta ggtgaattta gtgcagagca tattattcaa caaaatggtg gaatcatgga
      781 tactgaccaa tataatattt atctagacca atctatttct caatatagaa attttggttc
      841 tatattagct ctgttaggag gaatagggat attgtttgat aaatatatca gaaaatacta
      901 gagattatgt tttgtctaaa ataaattctt atgaggagat aaaaaatggc aacagaaata
      961 tttactgtaa aaattacacc cttagaggca caaaaaatga taaaagaaaa tcttgatgca
     1021 gatttagttt ttagtgattc ttataattta ggagatgata aatttatact aattacaact
     1081 tttgagaagt attatgcaag aaataatagt gatgcaggat taatagtagt ttatgaaaat
     1141 accacaggaa aaacagttat taaagctact tcaacaggca gtgcaattgg aattttaaaa
     1201 attgattggg gtgcaggtgg aaatttactt aaaagagtaa aaaacatact tgctgattat
     1261 attatttaat tattttatct aactgataat atgtcaagaa aatctcgtaa aagaggttaa
     1321 gaacattttt ataatatata gataaataag aatttgaagg taaagttttt aaggtataaa
     1381 gaaaaataat ataataaaac gaattggctt tataattctt tataaggcaa agagcacatt
     1441 tctttatata attgttgg
//
LOCUS       DAMJYX010000243         1421 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJYX010000243 DAMJYX010000000
VERSION     DAMJYX010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.248"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            137..946
                     /locus_tag="PC479_003616"
     CDS             137..946
                     /locus_tag="PC479_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806108.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            988..1254
                     /locus_tag="PC479_003617"
     CDS             988..1254
                     /locus_tag="PC479_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2806109.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          563 a          161 c          196 g          501 t
ORIGIN      
        1 gagattttac tttacaatta ttgtatctaa atttaacaaa atatcttatt agaatatttc
       61 aattaaaaca aaactggttt attatataaa aaataaatta gtatacttat tttttatata
      121 tcaggaggat atacttatga ataaaataat caataacatt ttaaatacct gcaaacagta
      181 tacatatatt ttgccacata aattgttaag acagtatata gcacactata caatatctat
      241 tccagataca agtattaaag aaaatcttac attaatacct gatgctagtg gatgtatgat
      301 atttaaattt gacaaaaaag gtatagaaag cgcattctgg ggagccacaa caaaaactac
      361 tattgtaaaa aatgatatag aaaatgtact ttttagagta ttcgttgagt ttcgcccagg
      421 tggagtatat tatttgacag gtctttctca aagagaatct acagatttaa aaatttcact
      481 tgaagatttt aacacattat ttagcttaga ggttaattcc atttttgaaa gaacaagtac
      541 tataaaggaa ttagtagaac aattagatat gttattctta tcatacttat tgaaatcaaa
      601 tatagtagat atgactatac caattcttga aaatgctaga aaacaaaact ctattatgtc
      661 tgttaaaaac atttcacaaa ttagttgtta tagtgaacga catttaaata gaatttttaa
      721 taattctctt ggtatgagtg ttaaatcata ccttagatta cttcgtataa accttgtatt
      781 acaagaaata caaaataata aaataccatt tgcaacttta gcacaagata ttggttatta
      841 cgaccaatct cattttataa atgattttaa atctatttgt ggtgttaatc caacaaccta
      901 tataaaaaat ttgtccgatt tttacaatga aaagtataaa ttttgatata caatcatata
      961 aaatttatat ttaaggagtg acttaaaatg atagaatcaa gatgtggaat tttatgttat
     1021 gaatgtggat acaaggaaaa agtcaattgt aaaggttgta cagagattga gaaaccattt
     1081 tggggtgaac aatgtcctgt aaaatcttgt tgtgaagata aaaaccttac acattgtggt
     1141 ctatgtgata catttccatg tgaaatgctt aatcaatttg catatgacaa agaacaaggt
     1201 gatgatggca aaagaatttc tcagtgcaaa aaatgggctg tactagcctt ataatatact
     1261 ctcttgttat aaaataagtt tttgtaaggt aaatttttta aactgttgta gaaatttctg
     1321 caacagtttt tataattaaa tttaaaggtt atttaattaa attaatagaa tagtaattta
     1381 ttgataaaat ctgattacta aaattggaaa tataaaatct c
//
LOCUS       DAMJYX010000244         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJYX010000244 DAMJYX010000000
VERSION     DAMJYX010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.249"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            27..101
                     /locus_tag="PC479_003618"
     tRNA            27..101
                     /locus_tag="PC479_003618"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC479_003619"
     tRNA            116..200
                     /locus_tag="PC479_003619"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC479_003620"
     tRNA            210..293
                     /locus_tag="PC479_003620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC479_003621"
     tRNA            323..399
                     /locus_tag="PC479_003621"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC479_003622"
     tRNA            407..482
                     /locus_tag="PC479_003622"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC479_003623"
     tRNA            571..659
                     /locus_tag="PC479_003623"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC479_003624"
     tRNA            663..738
                     /locus_tag="PC479_003624"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC479_003625"
     tRNA            745..818
                     /locus_tag="PC479_003625"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC479_003626"
     tRNA            833..909
                     /locus_tag="PC479_003626"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC479_003627"
     tRNA            933..1009
                     /locus_tag="PC479_003627"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC479_003628"
     tRNA            1017..1093
                     /locus_tag="PC479_003628"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC479_003629"
     tRNA            1102..1177
                     /locus_tag="PC479_003629"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC479_003630"
     tRNA            1185..1258
                     /locus_tag="PC479_003630"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC479_003631"
     tRNA            1265..1336
                     /locus_tag="PC479_003631"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJYX010000245         1293 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJYX010000245 DAMJYX010000000
VERSION     DAMJYX010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.250"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            156..1253
                     /gene="ychF"
                     /locus_tag="PC479_003632"
     CDS             156..1253
                     /gene="ychF"
                     /locus_tag="PC479_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2806110.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT          500 a          143 c          240 g          410 t
ORIGIN      
        1 aatcaatatg ctttaaagtc tagtttacaa gcttgatata tatgatgtat aatcaatatg
       61 tatgtctata attaaaacaa ttttgcaatc ctatagattg attgtttaaa gttatataaa
      121 gaatataaaa aagaataaga gagaggacga ttaatatgaa attaggtata gttggtttac
      181 caaatgtagg taaaagcaca ctatttaacg caataactca agcaggtgca gaatctgcaa
      241 attacccttt ttgtacaata gacccaaatg tgggagttgt atctgttcca gatgaaagat
      301 taaacaaact acaagaatta tataattctg aaaaaatagt tcctactgct atagaatttt
      361 gtgatatagc aggtttagtt agaggagctt ctaaaggaga aggtttagga aataaattct
      421 tatcacatat aagagaagtt gatgcaatag ttcatgttgt tagatgtttt gaggatgaaa
      481 atgttgtaca tgtagatggt tctgtagacc cacttcgtga tatagaaact ataaacttag
      541 aattaatttt ctcagatata gagattcttg aaagaagaat taataaaact caaaaagctg
      601 ctaaggctga taaaacttta ggttctgaac ttgatttatt aaaatcaata atgtctactt
      661 tagaagaaag taaatgtgta agaactatgg aatttacaga agatgaacaa acattcgtaa
      721 attcattgga tttacttaca tctaagcctg taatatatgc ttcaaatgta tcagaagatg
      781 atttagctga taatggagaa aataataaat atgttcaaca agttaaagca tttgcagaaa
      841 cagaagatgc cgaagttgtt gtagtatgtg cacaaataga agcagaaatt tctgaacttg
      901 attctgctga agaaaagaaa gagtttttag aaacattagg tcttgaacaa tcagggcttg
      961 ataagcttat aaaatcttct tatgctttac ttggtttaat ttcattctta actgctggtc
     1021 caaaagaagt tagagcatgg actataaaag ttggaagtaa agctcctcag gcaggaggaa
     1081 aaatacattc tgatatagaa agaggattta tcagagcaga aacaatagcc tttaatgatt
     1141 tggttgagca tggtactatg gctgctgcta aagaaaaagg gttggttaaa ttagaaggta
     1201 aagaatacat agttaaagat ggagatgtaa tattatttag atttaatgtt taaaatgatt
     1261 aatcgtatac actttgacta ttatatataa taa
//
LOCUS       DAMJYX010000246         1286 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJYX010000246 DAMJYX010000000
VERSION     DAMJYX010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.251"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..569
                     /locus_tag="PC479_003633"
     CDS             <1..569
                     /locus_tag="PC479_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HDF2806111.1"
                     /translation="EAIEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPIT
                     VVVEADEADCYYKSAEAGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVS
                     APDWVSAKGMRTLGNPLNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENS
                     RILLISTEGDTDPDKYRSIVWDGEYPSI"
     gene            696..>1286
                     /locus_tag="PC479_003634"
     CDS             696..>1286
                     /locus_tag="PC479_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2806112.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          488 a          143 c          291 g          364 t
ORIGIN      
        1 aagaagctat agagcaatta aaagaatatg gagtagatag accaacacac gtatttgttc
       61 aagcaggagt tggttcactt gctggtgcag ttcaagggta tgttgcttca atatatgatg
      121 aatgcccaat aacagtagta gtagaggctg atgaagctga ttgttactat aaatcagcag
      181 aagctggaga tggaaaacca agatttgttg gaggagatat gccaactata atggcagggc
      241 ttgcttgtgg agagccaaat actataggat ttgaagtatt aaaaaatcat gcagcagcat
      301 ttgtttcagc accagactgg gtttctgcta aaggtatgag aacattaggt aatcctttaa
      361 atggtgatga aaaggtaatt tctggagaat ctggagcagt tacaactggt cttttagtag
      421 cagctatgga aagagaagat ttagcagatt taagaaaaga tttaaaatta gatgaaaatt
      481 caagaatact tttaataagc acagaaggag atactgaccc tgacaaatat agaagtatag
      541 tttgggatgg agaatatcca agtatataag gaattactaa aatgcaatta ttagatattg
      601 gtttacatat ttagaagata tataagttct aaaatttaga aaatatataa gtatataaaa
      661 tacatacatt ataaaaaatc tagggggata atgaaatgtt aaacgaacaa agaaaacaag
      721 aagtaactga aatatgccaa aaattaattc aaaatcctag ttcttctgga aatgaagaag
      781 gtgttgtaaa agcaatagaa gagtcttttg aaaaattagg ttttgatagt tggtcaagag
      841 atagatatgg aaatatagta ggatgtataa aaggtaacaa gccaggtaag aaaatattat
      901 ttgatggaca tatagataca gttccagttc cagatgcaag taaatggagt gttcctcctt
      961 ttgaaggaaa aatagttgat ggaaagatat atggtagagg aacttctgac atgaaaggtc
     1021 aatatactgc aatgatgtct gctgtagcgt attttgctaa agacactaat agagattttg
     1081 ctggagaact atatgtagca ggtgttgttc atgaagaaat atttgaaggt gtttcggcta
     1141 gagagataag taaggctgtt caacctgact atgttgtaat aggtgagtct tcagagttaa
     1201 acttaaagat tggtcagaga ggtagaggag aaatagtagt agaaactttt ggtaaaccag
     1261 ctcactctgc taatccagaa aaaggt
//
LOCUS       DAMJYX010000247         1283 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJYX010000247 DAMJYX010000000
VERSION     DAMJYX010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.252"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..458
                     /locus_tag="PC479_003635"
     CDS             <1..458
                     /locus_tag="PC479_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2806113.1"
                     /translation="FFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKVIALI
                     ARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECINGYNLN
                     TNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            469..684
                     /locus_tag="PC479_003636"
     CDS             469..684
                     /locus_tag="PC479_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806114.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          466 a          146 c          159 g          512 t
ORIGIN      
        1 aattttttag taatatatta tcagtatttc tatattttct attatcactt ttaggagttg
       61 ctggtataat attcttatgt atgataccta tgtttttata tgattttagt tctgacaaag
      121 taatcgcttt aattgcaaga atactatatt tttctgacta ctttatagtc atcttattct
      181 tgatatttat aattaatagt actaagtatt ccccattcgt acttgaaaat gtaaaaaggt
      241 ttaaggtaat gggattttgt ttattgataa acactataat tgaatgtatc aatggttata
      301 atttaaatac taataatata agaatttttg gaactgatag tggcggtatc tcaccactta
      361 tgattatctc ttttatattt gcactaatgt gctttgtaat tgcagaaaca tttgataaag
      421 ctataaaaat aaaagaagat aatgatttaa cgatatagag gtgatgttat ggcaatagta
      481 gttaacctag atgtaatgat ggcaaaaagg aaaatatcac ttaaagattt ggcagaaaaa
      541 atagatatta caaatgcaaa tttatcaatt ttaaaaactg gaaaagcaaa agctattaga
      601 tttaccactc taaacgaaat ttgcaaagct ttagactgtc agccaggtga tatacttgaa
      661 tatgtagaag atgaaaatga ttaaaaaaga gtgtttagaa acactctttt ttattatact
      721 atatgatttt tattctttta ctatattatt gacattaata ttaactttgt ttaaagtcat
      781 aacaaaatat gttctaagaa agaaactgtt cctttatcta tttgttatcc attacctata
      841 agaactgtat cacactaaat aatactttta aattgataat tttaccacag attcctatat
      901 acattctttt ctacctaata tttatttaaa ctgttagcaa aacatggaac tttttcattt
      961 acttatacct ccatttatat tattgcatag tttatagcta cgtgttataa attacattat
     1021 tgatgatttt atctaacatt tgtaatcttt tagatactct ttgaagttac aacactgtta
     1081 tactctaatt aagtaaacta aatctgatac aagttggtga ttatatgaaa agaaaacttt
     1141 caacctattt taaaggatat attttaggtt tatgtatagc atttttatta tttactttta
     1201 tattaaaaaa attaattata gaaaatatca ttggaataat aattggaact ggaattgcat
     1261 ttttactgat ttacctattt aat
//
LOCUS       DAMJYX010000248         1240 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJYX010000248 DAMJYX010000000
VERSION     DAMJYX010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.253"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            36..731
                     /gene="cas6"
                     /locus_tag="PC479_003637"
     CDS             36..731
                     /gene="cas6"
                     /locus_tag="PC479_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2806115.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            722..>1240
                     /locus_tag="PC479_003638"
     CDS             722..>1240
                     /locus_tag="PC479_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HDF2806116.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLN
                     KIRLWIKTNIFDL"
BASE COUNT          543 a           93 c          191 g          413 t
ORIGIN      
        1 taatatggat tatcagaaaa tgaggaggtt gttaattgat aaaatgtctt gaaatgtctg
       61 taaaagtgta tcttcttgaa aatatttctt tagataatac atcaaaagaa atacgtaaat
      121 taatagatga tacattaatt atagaagaaa aatataagga atttcataat aaagctggat
      181 ttaagaatta ttcttttaat ttattatacc cattagaaaa agataaggtt tataagaagg
      241 attgtatata tacttttatt attagaactg tagatagtga attaggaatg tattttatga
      301 ataatttaaa aaatcagcat tctgataaaa taaaggttat tacaatgagt aaaaaagtga
      361 tccctaaaaa acatatagaa agtatttact ctgtcactcc aataatagct aaatttgagg
      421 atgggtattg gagaagtaat aaaacagtag aagatttaga aaaaagaatc agagaaaatt
      481 taataaaaaa atataatgaa tttactaaca gtaaaataga tgaagatttt gaattatttc
      541 aagttattaa atttataaat aggaaaccta tttctactaa atataagaat atatctttac
      601 ttggtgataa aataagtttt aaagtatcag aaaatgatat ggctcaggaa attgcaaatt
      661 ttgcacttgg agttggagtc ggagaaatta attctcgtgg atttggtttt gtaaactata
      721 gatggttata ggaggtgaaa acttgttaaa ggattgttta gagatttttg aaaaagaata
      781 caaatcttta ggaaatagat tgataactga tagctatgtc ctatcctatg gaacatatat
      841 attggtgaaa aatttttcga atatagatat attagaagtt tctaaaaata atgtggatag
      901 tacagaagat atataccata aattttgtga actggattat ttatcaaaac tggtgtctat
      961 agataaatca gttgattcca aaaaaataat acatagtaat aattatcttt cattttttgt
     1021 aaaaaaggaa aacttaagac ctgataaaaa taataatgta aaacttacag aagaaattat
     1081 tgataattat tatgatgctt tgttaaaccc aagaattaaa tataaatctg ataaaagaaa
     1141 aaaagaaatc tatgattcaa tagaaaataa atatggtaaa gtggatgaaa ataaattaaa
     1201 taaaataaga ctatggataa aaacaaatat atttgattta
//
LOCUS       DAMJYX010000249         1221 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJYX010000249 DAMJYX010000000
VERSION     DAMJYX010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.254"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..260
                     /locus_tag="PC479_003639"
     CDS             <1..260
                     /locus_tag="PC479_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806117.1"
                     /translation="KNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYILEN
                     KKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(697..1074)
                     /locus_tag="PC479_003640"
     CDS             complement(697..1074)
                     /locus_tag="PC479_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2806118.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT          505 a          169 c           99 g          448 t
ORIGIN      
        1 taaaaaatac aaaacaaatt acagtagaag ttaatgaaat tttattacaa gaaatgtcta
       61 agataactaa tgacgaaaat aatattttga cagatttgat aaatgaatca attgtaaatt
      121 atattcttga aaacaaaaaa cataatattg aaaataaaga tttagaagaa gtgattaaat
      181 tttacaaaaa agaaaatact gaaaaaccta aaagtgcagc aagagctata gctgaagcaa
      241 aaactcatac tctgtattaa tcaaataaaa aagttcctaa aaactaggaa cttttttata
      301 ttaataatta ttaatatatc tcaatattta tctcaaatct atcaatataa gcatgtttaa
      361 tacaatctat tgtatactta tcttacccta gaaataatca aaacaaaaat aaaattatgc
      421 cctttatatc taaaacaatt aatttacatg attaatatac aaaaattctg acttttaaat
      481 tcgtatacaa tatcaaacta aaataatatt aatgacaaac ccaatataaa ttaaattcaa
      541 acaggtttga ttttacatca aacttattgt attaatatta aataacaaga caaaaatatt
      601 tataaaaatc acaaatttta cataaaactt tatatttgat tcatttaact aattattgta
      661 aaaactattt attttatgat aattaactac accaaattat tcatcttttt taatatcctt
      721 aacccatttt tcaacagagt tcaattcttc ttcatttacc ccctttttga acaaggattt
      781 cataaaactc tcaatagaac ttccatgcat attatctaaa aaagtttttg tttcaaaatg
      841 taaatactct tcttctccaa taagaactgt ataatgtgta tgtctatcaa tttttcttgc
      901 acttaagaat tgcttattta ctaatcttga taaaagtgtt agtgttgtag tttgcttcca
      961 attatatagt ttttccatag cttcaatgac atcttttgat gctactgtaa aatctgaact
     1021 ccaaataaat ttcatgactt ttaattctgc tggtggtatc ttagtaatag tcatatgatt
     1081 ttacactcct ctcaaacttc tacataatta aatactatat taatagactt ttcgagtcaa
     1141 aggttttatt taaaatatct catccaaatt tattaataca aaaaatttat atttttattc
     1201 aaaattaaac acacaatcta t
//
LOCUS       DAMJYX010000250         1146 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJYX010000250 DAMJYX010000000
VERSION     DAMJYX010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.255"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            14..1054
                     /locus_tag="PC479_003641"
     CDS             14..1054
                     /locus_tag="PC479_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2806119.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          450 a          140 c          191 g          365 t
ORIGIN      
        1 aggaggaaac aatatgaatt taaatttaac taatactata aatttaatga aaaaatattt
       61 ggacattcca agcccagctg gatatactga aaatgcagtt ttagaaatca aaaaagattt
      121 tgaaaattta agtcttgaca ctattcttac aaaaaaaggt gcacttatag ctactcttcc
      181 tggtgaagat gattcaaatc aaataacaat atctgcacat atggatacac ttggagctat
      241 agtaaaagaa atatcatctg atggtactct taaataccat aaagttggtg gtggatgttg
      301 ggcggctata gagggagaaa actgtactgt tataactaga aaaggtaaaa aaatcagagg
      361 aagtgttgta tttaagtatg cttctactca tatatatggt caaacaaaag ctgccactga
      421 aagaaatgaa gaaacaatga ttattagact tgatgaaaaa gtatttacaa agcaagatgt
      481 attagattta ggaataagtg ttggggattt tgtatgccta gaccatagat ttgaggctac
      541 agaatctggt tttataaaat ctagatatat agataataaa tcagctgtag caatggttct
      601 tgaaatatgt agatatttta aagaaaacaa tttaacacct aagcatacta ctaactttta
      661 tataagtaat tatgaagaaa ttggacatgg tgtttctaaa tgtttacctg aaaaaacaaa
      721 agaatttgta gcaatagata tagcacctac tggaccaagt caaacatcta gtgaacatgg
      781 tgtaactata gttgcaaaag ataatttatc attatatgat tttaatttaa gaaacaagct
      841 tgtcgatgtt gcagaagaca ataatttaga ctacgctgtc gatgtattta ctttctatgg
      901 ttcagatgct tcagaagcta ttcgttgggg agaagatgtt caaatagcat gtgttggtcc
      961 tggaacaaac aatagtcacc attatgaaag aacacatata gaagctattg aaaatacact
     1021 aaaattactt ataaattata tgcttaaaga ataattaatt atatatttta ccaaagtcaa
     1081 ttcttaacaa ataattaact ttaattttta tttttataca aaaattggtt tttctataga
     1141 cttaat
//
LOCUS       DAMJYX010000251         1063 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJYX010000251 DAMJYX010000000
VERSION     DAMJYX010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.256"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            298..705
                     /gene="tnpA"
                     /locus_tag="PC479_003642"
     CDS             298..705
                     /gene="tnpA"
                     /locus_tag="PC479_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2806120.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            707..>1063
                     /locus_tag="PC479_003643"
     CDS             707..>1063
                     /locus_tag="PC479_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2806121.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNG"
BASE COUNT          458 a          116 c          179 g          310 t
ORIGIN      
        1 aaacttcaac gactattcct cttgagggaa gtacactaca agctatcggt agtggaagtg
       61 ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttgc atgaaagtgt
      121 aagaagttca taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa
      181 aatgataaaa gttaagattt gcgtttctta cgtatgttat ttaaaaataa atataaaaac
      241 cttaagaata cttgttttaa gtaattacta tgttaatatt atgttgggtg atgaaaaatg
      301 gaagtgactc atggtagagg gtatgtgtat ttagtacaat accatatagt atggtgtgta
      361 aaatatagac atccagtatt aacaaatgaa attgaagaag atttaattaa tatattaaac
      421 aaaatagctg aagataataa atttcaaata ttagaatgta atagtgataa agatcacata
      481 catttactaa taaattgttc accacaacac tatataccag atataataaa agcattaaaa
      541 ggtgtatcag caaggctgtt aatgaaaaaa catggagaac agttgaaaaa gaaattatgg
      601 ggaggacatc tttggaatcc tagttatttc gttgctactg tatcagaaaa tacagaagaa
      661 cagataagaa aatatattaa taatcagaaa aaacaagtaa agtgaggtgg cagtagtgga
      721 aaaagcttat aagtttagaa tgtatccaaa taaaaaacaa caagagttaa ttaacaaaac
      781 ttttggatgt tgtagatttg tatataataa gtatcttgct aaaagaatag aagtttataa
      841 aaacaataaa gaaactttta cttataaaca gtgtagttct gatttaacca atttaaaaaa
      901 agaattaaaa tggcttaagg aacctgacaa attttcacta caaaacgcct taaaagattt
      961 agataatgca tataaaaaat tctttaaaga aaaagcagga tttcctaaat ttaaatcaaa
     1021 gaaaattaat agattttcat ataaaactaa ctttacaaat gga
//
LOCUS       DAMJYX010000252          970 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJYX010000252 DAMJYX010000000
VERSION     DAMJYX010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.257"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            305..772
                     /locus_tag="PC479_003644"
     CDS             305..772
                     /locus_tag="PC479_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDF2806122.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          374 a           64 c          162 g          370 t
ORIGIN      
        1 aatattatta gtgtatatta atattccctt gaaattaaaa aaacatagat atttcagtaa
       61 tctaaaatag tctacaaaaa taacagtttt agcaaaatta ataagaatac atatagattt
      121 aaacctaaaa gtatgaacat gtctgttttt tacaagatta aaatttatat tatatgataa
      181 aattaactta actatttcaa taagacttat aatttttttc tttatactag accttattga
      241 aacctttata gtttaattct aattatatat tataaataaa gtatgtgtat attaaggagg
      301 taatatgaag tttttaaatg ttttagtagt agttgaagat attgagaaat caaaaaagtt
      361 ttattatgat gttttggggt taaaggtaat ctgtgatttt ggagaaaatg ttgtattaga
      421 aggtaatata tcattacaag aaaaaaagct ttggttagaa tttattaata agagtgacag
      481 tgaagttaag ttcaatggaa atgatgctga gttatatttt gaggaagata attttgatac
      541 ttttgttgaa agattgagta caatgaaaga tatagattat gttcatctag ctatagagca
      601 taggtggggt caaagagcaa ttagatttta tgatttagat gggcatatta ttgaggttgg
      661 agaaactatg tcatctgtgt gtagacgttt tttagatagt ggtctttcaa tagatgaggt
      721 agctaaaaga atggatgtaa ctgtcgaata tattgaatca gttttagaat agatggatta
      781 tattaaagta aaattgaggt gaaggctgta agtaaacttg tgtgaaaaca aatttgctta
      841 cagtttttat ttttctaaaa taaagaatat aatacttgtt tagctttatt tgagttttta
      901 aaaatattat gatatttctt atatatttat actaaagttt aaatttagaa aaaatatata
      961 aaagatagtt
//
LOCUS       DAMJYX010000253          958 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJYX010000253 DAMJYX010000000
VERSION     DAMJYX010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.258"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            20..529
                     /gene="vanZ1"
                     /locus_tag="PC479_003645"
     CDS             20..529
                     /gene="vanZ1"
                     /locus_tag="PC479_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2806123.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(631..825)
                     /locus_tag="PC479_003646"
     CDS             complement(631..825)
                     /locus_tag="PC479_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2806124.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
BASE COUNT          351 a          112 c          119 g          376 t
ORIGIN      
        1 tatagtaagg aggtaataaa tgaaatctag aaaacataat attacaaaag gattatttat
       61 agtttacata attattttaa catggataat actatttaag ttgcaatttg atatctcatc
      121 tcttgaaacc atgaatctta gaagcataaa cttaatacct tttgctgggt cacttataat
      181 taataataga gtagatatat cagagattat attaaatgtg gctatatttg taccatttgg
      241 gatatatgtg tgtatgctaa aagaagaatg gagctttata aaaaaagtta tacctatttt
      301 tataactagt ttggtatttg aaacacttca atatatattt gcactaggtg caagtgatat
      361 aacggatttg ataggaaaca cactaggagg aatcatagga atagctgttt ttatgctatt
      421 atcgaaaata tttaagaata ataccataaa aattatcaat gtattggcat tgattgtgac
      481 aattattgtt gtattatttt taggattagt tatttttgca aacttataga aataagtata
      541 tatgtttagc ttaaaataga acgtaagata cagttacata taaaattatt tacacataaa
      601 aagcacctat acaggtgctt tcctaaatta ttaaagaatt attgtatctt gaaactcttg
      661 tatgttcaat tcttgatttt ctcttgtttt ttctaataaa tctaacgcac aatttataca
      721 aactttaatt tctgcattgg tatttttctc ataataagtt atatatttat ttctatattt
      781 atctatttct tttccacaac gactacacat tacaattacg ctcatatctt agttcctcca
      841 tctctaatta ttcttaatat attcaatagt atcacttttc tgaaaataat gattgttaaa
      901 actgtatcaa atttagattt actttttatt ataatgcatt ttaataaaaa aataaagg
//
LOCUS       DAMJYX010000254          894 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJYX010000254 DAMJYX010000000
VERSION     DAMJYX010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.259"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            127..>894
                     /locus_tag="PC479_003647"
     CDS             127..>894
                     /locus_tag="PC479_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2806125.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISD"
BASE COUNT          327 a           99 c          167 g          301 t
ORIGIN      
        1 atgaataaat caatagaaaa tcttctaagt tgcttttaaa taatgacttt atggttcaat
       61 tgtactaaaa tagtatatgt aggttacaaa taatctaata aatattaaag tttggaggta
      121 atagatatgg cagattgtaa ttcatgtcca tcaaagggaa attgtaatag tcaatcaaat
      181 tgttctattg aaaataaccc taacaataaa tttggaaaaa taattggtgt tatgagtggg
      241 aagggtggag ttggaaaatc aactgtaact gctttacttg ctaataagct aaacaaaatg
      301 ggatataagg ttggtatatt agactcagat ataactggac caagtattcc tagacttatg
      361 ggtgtaaaaa atgtaaaagc ttattctgat ggctcttata tttaccctgt agaaaactca
      421 aataacataa aagtaatgtc tataaacctt atgatagatg atgaaaatga acctgttgtt
      481 tggagaggac ctttacttgg aggggttgta aagcaatttt atactgatgt tctttgggaa
      541 gaattagact atttattaat agatatgcct cctggtactg gagatgttgc cttaacagtt
      601 atgcaatcta ttcctataag tggaatagtt atggtttcag tacctcaaga tttggtatct
      661 atgattgtat caaaagctgt taatatggct aaaaaaatga acataaatgt ccttggagtt
      721 attgaaaata tgagttatat ccaatgtcct gattgttcta aaaaaatcaa gttatttgaa
      781 ggagaatcta ctgaaaagtt tttagatgat ttagacttag agcttttagg agaacttcct
      841 atgactaaag aaattataga tattactcat aatggagtta cagaaattag tgat
//
LOCUS       DAMJYX010000255          816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJYX010000255 DAMJYX010000000
VERSION     DAMJYX010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.260"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
BASE COUNT          275 a           93 c           82 g          366 t
ORIGIN      
        1 ttatatatat tataacaatt atagcaaatt tttagggtat aatttttaaa attatattct
       61 actatcaaaa gatatgaata aatgattgta tatattatat caatttttat atttcatagt
      121 ccattttttc atcacatttt cgcttgtcaa gaataatatc tttaagagtg aagcgtttga
      181 tgtaaatctg gactttgttt ttgagacatt tttgaaatat ttattaaatc tttcgattat
      241 acttgtaata tatattatag atattactta ttttagtaaa actaaaggat gatgctacaa
      301 cattccagtt atttcccaaa attttaattt ctgaagtata ctgtttacat tatttttaat
      361 ctattttatc taaagtcgat aaggcttctt attcattaac tgacctatat acagatttaa
      421 aattacttat taattctttt gagtccttgt atgaaacata tttaaagtat ttcttatcta
      481 atgtattaaa catttttgta ttacagaatg aggataactt gtagctattg tctctttgaa
      541 ataagtcatc tgtgtttact cctaaaacta cttatatctg tagattttat aagttctcca
      601 tcttatataa tttctttgat taattgtttt cttaaattat tcataaaaaa atctccgtta
      661 caatatttga ttatatttaa atcattgcta taaagaagac ttttattaac tttgaaaaac
      721 aaattatttt aattgctggt gattagttgc taccttcttt tgatattaat tctattagat
      781 tttatatagt tatatatctc tttctaacta agtaat
//
LOCUS       DAMJYX010000256          812 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJYX010000256 DAMJYX010000000
VERSION     DAMJYX010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.261"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(97..705)
                     /locus_tag="PC479_003648"
     CDS             complement(97..705)
                     /locus_tag="PC479_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2806126.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
BASE COUNT          306 a          134 c           68 g          304 t
ORIGIN      
        1 aaatataata acactactaa tattttaaat atcaatagtg tcataaaaac ttttcagatt
       61 aattaaatct ttctatatat tttataataa tctaaatcat gctaaatatt tagacagaaa
      121 acttttttct ttaatagtaa atacttttgt ttgaatatca gaataattat ttgaatatct
      181 atctataaaa ccatgatttg catctaaaat ctcaatttgg atattttgtt tcctagaaag
      241 ttggtaagaa atagaatcaa catcaaatga atcgtgcttt gcaaatacta ataaggtagg
      301 acattttgga attacatcca tgtaatctct aatccgagag ccataacaac aaattatgcc
      361 atcacaaaaa gaatcttcac tgcatctcca tgcaatagtt gctcccacgc tatacccagc
      421 aacaaataca tatttatact ctgattttaa atcatttata agcttttcaa cttttttata
      481 aatatcaaaa ccaacattat ttataaaaaa atcatatgcc tcttggactt ttgaatatgg
      541 aaaaactctt ttttctccca gaagttcagg acaaaataca tcatatccat atttataata
      601 cttttgacaa acatcttcta taaagctatt aatcccatag atttcatgca aaacaacaat
      661 agctacatca cttttatgta tatactttat cagattatcc accaccttta caaaacagca
      721 aattttttat acttagttta atatattctt ctttacatgt caatttactt ttctaaaatc
      781 aaatttattt ttggattaat catttatttt ta
//
LOCUS       DAMJYX010000257          801 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJYX010000257 DAMJYX010000000
VERSION     DAMJYX010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.262"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            70..612
                     /locus_tag="PC479_003649"
     CDS             70..612
                     /locus_tag="PC479_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2806127.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          337 a           63 c          141 g          260 t
ORIGIN      
        1 attagtaata actttaaaat tatattacta ttatataaaa attggcttaa aagaaggtga
       61 aaaaagtaaa tgaaaatatg cataacagta ggtcatagta tactaaaagg tggtaaaagt
      121 acaggtgtaa atggtatagt tgatgaatac aattataata aaaaattagc tcctatgtta
      181 gctgagatgc ttataagtca aggtaataca gtagatgtaa taatttgtcc agaaaggcac
      241 tttatgtcag aaagagaaga atttttctat agagttccaa aagtaaacag tggtaaatac
      301 gatttattag tagagctaca tttaaataaa gctgatggaa cacaatgtgg tacagaagtg
      361 ctttactatg gcactgaagg attagaatat gctaaaagag tatctaatag acttggagaa
      421 ttatttgaaa atagaggagc taaaaaaaga gaaaatttat atatattgag aaatacaaat
      481 ccagttgcaa tacagattga aagttttttc tgtgataatg tgaatgattg taacaaagca
      541 aatgaatcag gttatgatta tatagcacga ttgattacag aaggtatatt gaataaagat
      601 ataaatatgt gagattatgg tgatttaggt attgattaat accttttaat attagatata
      661 aataaaaata caatgtatta gtataattta aactttcaaa agatagatta gtttgattat
      721 tgacaagaga tacctccttg aaaatattga tttatcagta tatttaagga ggtatttaaa
      781 tataaaaatt taattaattt g
//
LOCUS       DAMJYX010000258          791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJYX010000258 DAMJYX010000000
VERSION     DAMJYX010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.263"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            36..>791
                     /locus_tag="PC479_003650"
     CDS             36..>791
                     /locus_tag="PC479_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2806128.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVN"
BASE COUNT          330 a           84 c          129 g          248 t
ORIGIN      
        1 ttactataat actatattgc gaaaagaggt agaaaatgtt tgcagaagaa agaatccaag
       61 ctatattaaa tatcctaaaa aaagaaggta gagttactgt caaagagctc agttctaaat
      121 ttaatgttac agaagattgt ataagaaaag atttaaaaaa tatagaaaag atttcttcaa
      181 taagaagaat ttatggagga gctgtactag ctagagaaac actagagaat caggatacaa
      241 aagacagaaa agaaattaat attccaacaa agaaaataat agcagaaaaa gcatttaatt
      301 taattgaaga tagagaaact atatttttag atatctctac aattaacatc ttgcttgcga
      361 agttgttagc tgaaagtaat aaaaaagtaa cacttgttac aaatatgctg gatatactaa
      421 atgtagttac ttcaaaacca aataatttaa atataatttc cactggaggt ttattaaatc
      481 tcagtttaga cggttttgtc ggtactccaa ctatagagtt tatatcgaaa tataagtttg
      541 ataagacttt tatgggaagt tgtggagtag atgtattcaa ctcttcactt acaacttttg
      601 aaattgaaga tggtcttacg aagaaggcta taataaattc aagtaaaaaa gtatttatag
      661 ttatggaaga taaaaagttt aaatttgatg gaaatttcaa gtttgctcat ttagaagata
      721 ttagttctat aattacagaa aaaacgccta ctcctgatat agtagatatt ttatcagaat
      781 ttaatgtaaa t
//
LOCUS       DAMJYX010000259          787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJYX010000259 DAMJYX010000000
VERSION     DAMJYX010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.264"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..>787)
                     /locus_tag="PC479_003651"
     rRNA            complement(<1..>787)
                     /locus_tag="PC479_003651"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAMJYX010000260          775 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJYX010000260 DAMJYX010000000
VERSION     DAMJYX010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.266"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(158..>775)
                     /locus_tag="PC479_003652"
     CDS             complement(158..>775)
                     /locus_tag="PC479_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806129.1"
                     /translation="GEQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYD
                     IEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNL
                     VYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFK
                     GIIDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          265 a          122 c           60 g          328 t
ORIGIN      
        1 ttttcattat caaaataaat ctaaacaatg aaaatatatt tgatttcata taaaaaaagt
       61 gtctcatgag ctaaaaagtt tattttgaga cactccctta cttatactta tctgtgatac
      121 atataaaagt ttaaagttct aaaattaatt ctaacactta atttgcaaca aaaaataact
      181 tttcttgtaa acttatatct ccatttatta gctcagcttc ataaatattc tttttaagtt
      241 tcatatcaaa tacttgaaat tttactggct tagttccgac tacatcaata atccctttaa
      301 atccttttaa ttctcctgat atactgttct taatacctga aatagaatat atcttatcat
      361 ccagtagctt aactttccta actccagatg atgcttcatt ataatctact tttaaatcaa
      421 gtatctcata atcttctaat ttaatctcat tagaattaat atcataaaca agattagtta
      481 tggaattact catatccgta actataagat tcaatttatt gttttttacc atataatcta
      541 gcttagtttt acttaataag ccaatctcct ttggatttgc attcttttct ttgtttagat
      601 tatccataaa tttattactt atattaaaag tgttatcttc tatatcatat ataataaagt
      661 tagagtaact atcatgtcct tcaatatatc catttttact tttattattt actatttcta
      721 agtataattt acttccaata gaaaatttaa tttcattgat attttgttgt tctcc
//
LOCUS       DAMJYX010000261          767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJYX010000261 DAMJYX010000000
VERSION     DAMJYX010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.267"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            202..708
                     /locus_tag="PC479_003653"
     CDS             202..708
                     /locus_tag="PC479_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2806130.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          307 a           77 c          129 g          254 t
ORIGIN      
        1 atactttata atcaatatat tttatctata ttttacactt ataaaatatg aaaactactt
       61 attgcgaaag taattgattt gttgtattat aatttacatg aattatgtag gacaatcaaa
      121 agtcagtatt ataagagtac atatattcaa atttttaaca attaatttat ataattaaaa
      181 aatgaagggt aggatgaagt catgggtttt agagaagtaa aaattgagga attacagttt
      241 aatccattta ctaagattgg caaagaatgg ttacttataa cagctggtga ttcagaaaag
      301 tttaacacaa tgactgctag ctggggtggt gttggagtct attggggtaa aaatgttgtt
      361 acaacttaca taagaccaca gagatataca aaagaatttg tagatagtaa tgatacattt
      421 acagttgcct tctttgatga aacgtataga gaggctttaa atatttgtgg tactatttct
      481 ggtagagata taaataaaat agaaaaagca ggacttacac catattttgt ggatgataca
      541 gtagcttttg aagaagctaa tatgattata gtatgtaaaa agctatatca tggtaatatg
      601 ccaccagaaa actttgatgc taaagaaaat gataaaaaat ggtatccaga gaaagactac
      661 catactatgt acatttcaga aattataaaa gttcttgtaa aagaataatt ataataaaaa
      721 atatatgaaa agaacttatt ataagtaaat gactgtgctt tcttatc
//
LOCUS       DAMJYX010000262          753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJYX010000262 DAMJYX010000000
VERSION     DAMJYX010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.268"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..>753
                     /locus_tag="PC479_003654"
     rRNA            <1..>753
                     /locus_tag="PC479_003654"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAMJYX010000263          731 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJYX010000263 DAMJYX010000000
VERSION     DAMJYX010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.269"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..307
                     /locus_tag="PC479_003655"
     CDS             <1..307
                     /locus_tag="PC479_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2806131.1"
                     /translation="SNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKIN
                     EVVLKNNKVITSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
BASE COUNT          323 a           57 c          113 g          238 t
ORIGIN      
        1 ttcaaatata atagataatg caatagaagc ttgtattaag attaaggaca caagtataca
       61 aaaaaagatt aaattaagag gaactgttat aaataggtta tttgtaataa aatgtgaaaa
      121 tacaaagata aatgaagttg tattaaaaaa taataaggtt attacaagta agaaagattc
      181 ctttttacat ggaataggaa taagtagtgt aaaaagttca gtagaaaaat ataatggaaa
      241 tgtggaaata tactcagata aaaacaagtt tacaattact atttatatac cattaactag
      301 gaactaattc tagttggggc tctaaattgc ccagttggag cagtagtatt gaaagataaa
      361 atagaagatg ctatctttaa ggcataggat atttggaggt gataaaaatg gttgaatgct
      421 atgctaataa gataacatcc tttttagtac tcaataaaat aatagaacaa aaaatgtgat
      481 ttatatttgt ttgaatttaa aatgttgata ggttttatag ttatattatt tgcatgatat
      541 atcttaaata aatttagaaa aatagagtta tttttatctg caaatgcgac cttagtatgg
      601 taacattatt gaccaaaagt tctatatagt ataaaaaaat aaaatgtaaa tttgataaac
      661 tttataacta ggaacataaa aacaacaatt aaataactaa ttagatagta aagttgatat
      721 tagtaataaa t
//
LOCUS       DAMJYX010000264          725 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJYX010000264 DAMJYX010000000
VERSION     DAMJYX010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.270"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..254)
                     /locus_tag="PC479_003656"
     CDS             complement(<1..254)
                     /locus_tag="PC479_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806132.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          296 a          109 c           86 g          234 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt tttaaaattt
      301 ggttttaaaa aatgattttg atataaaaaa accacacagt atatataact gtatggcaaa
      361 aattcgaata agccacacag tataatccat gtggctctat attatgatga taaaataaaa
      421 actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca taatcctaag
      481 gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct aactaaattg
      541 gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct ctccttttaa
      601 tttaataatt ttgaaaattt tgttttctgt tttgtatagt aattagttta atacagtaaa
      661 aaataaaagt caactataaa ttttgaaaaa ttatcaaatt atcagaaaat tgagtttaaa
      721 taaaa
//
LOCUS       DAMJYX010000265          720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJYX010000265 DAMJYX010000000
VERSION     DAMJYX010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.271"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
BASE COUNT          313 a           73 c           73 g          261 t
ORIGIN      
        1 ttagtatata ttaatcatgc tgaatttgtg atgacatgta tacaaaatat cgtttagtat
       61 tttctaataa aaaatattac tgaaattaaa aatctacaat agcatttaac aagtaaaaac
      121 atgtttacac aaatatataa tttaaggtct ttaaaataga gtatgtatta ttctaaaaat
      181 tagttatagt tatcacataa aaatagaaaa gaattttcaa aagatttaaa gaacatatat
      241 acttcaattg cagaagatgt aatgttaagt gaactagcat tcttcgagga aaaatagact
      301 caaaaaatta gaaaatagaa tcaggtttta gatgatttaa gtatttattt tcaaaaaaga
      361 aatttgattc taagttaata aagaaatatt taaaaaaatc aaaaaatttt cttaaataag
      421 taataggttt tgtaaaaatg gaaaaatatc aaaataagac tatgattttt aaaactatag
      481 ccttatcaat aaatttttat ttaaagaaca cacataaata tttttaaacc taatttatgt
      541 gaagttttat gtatttttta tataagtagt attctaatct tactcatcta atataccaaa
      601 aagactgtaa gtaagttcac ttttatgcaa acttacccac aatctctttt acacattaat
      661 tctatttatt aaaattaaaa atatttactg ctattctacc caataaagat gatactgttg
//
LOCUS       DAMJYX010000266          709 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJYX010000266 DAMJYX010000000
VERSION     DAMJYX010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.272"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(340..546)
                     /locus_tag="PC479_003657"
     CDS             complement(340..546)
                     /locus_tag="PC479_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806133.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT          269 a           68 c           88 g          284 t
ORIGIN      
        1 taattagttt taaatcaata gaatagtatg tatttatcta atgcggcaat ataatttaaa
       61 gctggtgttg tagaaaaact aagagtgaat atagcaaaga gaagcaatag tattttaaac
      121 tttgtctctc ttattttttg aaaactaaca ttaaaacata aggtataaat aatattatca
      181 tataaatgaa aacaaatttg aatttttctc tggtcgatgt atatagtaag tttgttgata
      241 tgagaaaaat tattattgat aatatacatg catagaaagg aaatggtata taatattttc
      301 ttaagtttct tttttaggca tttctattgg cattttaggc tatagtacta aatacattaa
      361 ccaacaaaat aatgaaataa atatatatat agaaaactta attttcgtgg gaagaaaaaa
      421 agcaataata gcaagtatta ttagagaata acgaataata cgagtaacat ttctttcttt
      481 ttctatgata gttaaatccc agtgattttt tataaattca aggtcttttt cttgactctg
      541 attcatttaa tcatctcctg ttaatagtat aattttaaag ctattacttt gttttatttt
      601 aacatattta tacactaaag tgaattttat gattagattt atttaattat ttctgaatta
      661 aaacttatta gaattctcta ttttgataat ttcaaactct attgattgt
//
LOCUS       DAMJYX010000267          664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJYX010000267 DAMJYX010000000
VERSION     DAMJYX010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.273"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..>664)
                     /locus_tag="PC479_003658"
     rRNA            complement(<1..>664)
                     /locus_tag="PC479_003658"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAMJYX010000268          660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJYX010000268 DAMJYX010000000
VERSION     DAMJYX010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.274"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..634)
                     /locus_tag="PC479_003659"
     rRNA            complement(<1..634)
                     /locus_tag="PC479_003659"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          151 a          175 c          122 g          212 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
//
LOCUS       DAMJYX010000269          660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJYX010000269 DAMJYX010000000
VERSION     DAMJYX010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.275"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..160)
                     /locus_tag="PC479_003660"
     CDS             complement(<1..160)
                     /locus_tag="PC479_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2806134.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYS"
     gene            453..644
                     /locus_tag="PC479_003661"
     CDS             453..644
                     /locus_tag="PC479_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806135.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          267 a           59 c           87 g          247 t
ORIGIN      
        1 tactatagta attttgacta ttgaatccaa ttagtacagc aacgtctaaa atagacaagt
       61 ctttttttaa tatatacttt ttgctttttt caactctaac tttacttaaa aaattagtaa
      121 aagtatatcc actttttttc ttaaataaag aaaaaaacaa cttttattaa gttgtaattc
      181 attacatata ttacttactg ttatatcttc tgagtaattt ttgtgaatat aatctatagt
      241 tttctttaag ttttctttac ataaaactaa gagttggtct atgtttcatt ttatcaaggc
      301 atatttatta aaaaatcaat ctctatttgt taaaataatt tatgaatgat gttagaaact
      361 gtaatatatt ggatataata taataaacag ataaactctg tattaagagg atatatagtt
      421 tatcttaatt ataggggaag tgaggatata ttatggaaag attaatggaa ctcatagaaa
      481 gaaaattttt aaaggatgta gaacttttag aattagaaga catagaaaat gtaatagaat
      541 gcaaaatgac acagccatat aatgctcatt ataatgaata tttagtaaaa gtaaaaaatg
      601 gagatgaagt agaagaatat tttgtgtatt taaaacatag ataaacataa aagaggttta
//
LOCUS       DAMJYX010000270          631 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJYX010000270 DAMJYX010000000
VERSION     DAMJYX010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.276"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            173..541
                     /locus_tag="PC479_003662"
     CDS             173..541
                     /locus_tag="PC479_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2806136.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT          270 a           51 c          107 g          203 t
ORIGIN      
        1 ttaaagtaac tttataaata aaaagtaata taaattgata aaattataaa tttatttata
       61 gtgaatatgg gttggatttt atagatacaa aaatagtata tatgatatgt agaccattag
      121 caggtttaaa gtagcaaaac atataaaata aaatttgaaa ggaagtataa aaatgacaag
      181 accatcaata tatcatagcc aaggatacac ttgtgcagag gcattaataa agtcttataa
      241 tgaggaacat aacacagata taccaatctc aataggaagt ggtatggggg ttggaatgaa
      301 tgttggaagt gtttgtggag ctgtaaatgc tgctgttgtt ataatagggt atttaaatgg
      361 gcgaaatagt aatttagatg aaaatatagc aaggaagtat tcaagagagc tattgaatag
      421 agtaagaaat aaatacagct cagaaatatg tgtatctctt aaaaaaaata aagtaacttg
      481 tgaagaaata ataaatttca catatgatgc tctaaatgat atgctaaaaa atcaaaaata
      541 attctaagaa acaagtattt tacattcgat agaatacttg tttttttatg atatttttaa
      601 taagtattag tttattataa tttttaaatc a
//
LOCUS       DAMJYX010000271          607 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJYX010000271 DAMJYX010000000
VERSION     DAMJYX010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.277"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            1..>607
                     /locus_tag="PC479_003663"
     CDS             1..>607
                     /locus_tag="PC479_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2806137.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNIS"
BASE COUNT          212 a           55 c           81 g          259 t
ORIGIN      
        1 atgttagatg catttataac aattgtttct tctattatag aaatatggtc atgtaaaaaa
       61 gtttttgatt atactagtca gtcaaagacc gacttaaaaa aactaaatat gtattttctc
      121 ttttctgttt ttctcattat ttcttcttta tatataaata taggagctaa tagtagaatc
      181 ttgatatgta tatttgtaat gttcgttttc tataagtcta actataaggt taatatatat
      241 aaatgtctgg taatctcatt tttttattgg ttaatattaa taggtataaa tggattaagt
      301 atgttattag tagcaacagt aagcaatttg gataatatga atgttttact tagtggcagt
      361 ttatatagat atgaagcaat attcttggga aagataatgt taggtatatt actttatgtt
      421 tataaggctt cttttaaaaa ttttaaaata agtaaaaaag agatatacat atgtattcct
      481 atgatagcaa atttattgtc attttttgta ctttatgatt atattattaa tcttgtaaaa
      541 atctctcctg aaagaagaac tgctttttta gtaatagttt tactattaat attatctaac
      601 atttcaa
//
LOCUS       DAMJYX010000272          602 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJYX010000272 DAMJYX010000000
VERSION     DAMJYX010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.278"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            96..386
                     /gene="cas2"
                     /locus_tag="PC479_003664"
     CDS             96..386
                     /gene="cas2"
                     /locus_tag="PC479_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2806138.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT          253 a           38 c           90 g          221 t
ORIGIN      
        1 tttaagatga gaaaggagaa aatactgaaa aataattatt ctattataat ttgccaaggt
       61 tttgtatatt tttcagttta aatattatat tacatatgaa aaagaaaaac aatttaaatt
      121 ataattatgt atttgtagtc tatgatgtag gagaaaaaag agtaagtaaa gtttttaaag
      181 tgtgtaaaaa atatctctcg cattttcaaa aatcagtttt tagaggtgaa ataactccat
      241 ctaagattat gagcttaaaa cgtgatttag aaaatattat agataaagat aatgattttg
      301 tatgtataat aaagttgatg aattctaata catttgaaga agaaatatta gggaataata
      361 gtaattgtga aaatatgata ttataaatct aattactata tttgtaaaaa ataatagctt
      421 attaaagagt agtaaaattg ggatataatt tatgatagag actgcaagta gatttttgtg
      481 aaaaggattt acttgcagta tttttgttat gtataactgt taacattagt gtacaaaaca
      541 ttatcaatag catttttcta taatgaagaa atactattat aatgggaaaa ttttattaat
      601 ac
//
LOCUS       DAMJYX010000273          594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJYX010000273 DAMJYX010000000
VERSION     DAMJYX010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.279"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..>594
                     /locus_tag="PC479_003665"
     CDS             <1..>594
                     /locus_tag="PC479_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2806139.1"
                     /translation="RDKQVPQGRILNATISKEPSGRYYVSLCCTNVDIEVFENTNNQI
                     GLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARL
                     QEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQL
                     KYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDL"
BASE COUNT          249 a           64 c          102 g          179 t
ORIGIN      
        1 taagggataa acaagtacct caaggtagaa tacttaatgc tactatatct aaagaaccaa
       61 gtggtagata ttatgtatca ttatgctgta ctaatgtaga tattgaagta tttgaaaata
      121 ctaataatca gataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag
      181 actttataga aaatcctaag tatcttaaga agtcattaaa caaacttgct aaattacaaa
      241 gagaattatc gagaaagaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg
      301 caagacttca agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta
      361 aattaataaa agagaatgat attatttgca tagaagattt acaagtaaag aatatgatta
      421 gaaatcgtaa actatctcgc ttaatttctg atgtgtcatg gtcagaattt gttcgtcaat
      481 taaaatacaa ggctaattgg catggtagac aaattgtaaa ggtaggtaaa ttctttgcaa
      541 gttcacaaat atgcaataaa tgtgggtata aaaacgagga aatgaaggac ttaa
//
LOCUS       DAMJYX010000274          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJYX010000274 DAMJYX010000000
VERSION     DAMJYX010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.280"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..>577
                     /locus_tag="PC479_003666"
     rRNA            <1..>577
                     /locus_tag="PC479_003666"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJYX010000275          544 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJYX010000275 DAMJYX010000000
VERSION     DAMJYX010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.281"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            29..223
                     /locus_tag="PC479_003667"
     CDS             29..223
                     /locus_tag="PC479_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2806140.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          212 a           55 c           58 g          219 t
ORIGIN      
        1 ttttcatatt aataggaggg attctacaat gataaattat atggtagaaa ttatatatag
       61 cgaaaaaata agcgaagtag aaaacatgtt gaaaattgaa aatatacaat atgaatttac
      121 ttctcgtgat ggctttatat tttttggttt ttatgatatg acagacgcat atagatttga
      181 gactttatgt tcagatgaaa aaattcgatt taaaagaata taatagataa aaaagtacat
      241 atttctaaac tatttgtttt tcaaatttag gttgtgtttt aatgtagtgt tgatattata
      301 aacaatttta ataataaaaa agtagggaca ttatattaat atgctcctac tttttactca
      361 tacttattaa tattatttat caaaattacc ctacaaactg gaatttatca ttctttcata
      421 attttaaact atcatattta aaatatataa aattctacat gatttttgta taaacataat
      481 atcaaccatt atccaattta aaaatatttt ttagtaattt ccatttcatt ttctcttaac
      541 atat
//
LOCUS       DAMJYX010000276          496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJYX010000276 DAMJYX010000000
VERSION     DAMJYX010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.283"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            <1..>496
                     /gene="tuf"
                     /locus_tag="PC479_003668"
     CDS             <1..>496
                     /gene="tuf"
                     /locus_tag="PC479_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2806141.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAMJYX010000277          493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJYX010000277 DAMJYX010000000
VERSION     DAMJYX010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.284"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="PC479_003669"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="PC479_003669"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="PC479_003670"
     tRNA            310..398
                     /locus_tag="PC479_003670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="PC479_003671"
     tRNA            410..486
                     /locus_tag="PC479_003671"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAMJYX010000278          396 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJYX010000278 DAMJYX010000000
VERSION     DAMJYX010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.285"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            37..321
                     /locus_tag="PC479_003672"
     CDS             37..321
                     /locus_tag="PC479_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806142.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          179 a           26 c           71 g          120 t
ORIGIN      
        1 aaaatggtgt aaatagaatg ataaggggtg gatgaaatgc ttgtatataa taaaagtttt
       61 tatcctaatg acatatttcc aagattagat ttttcaaaaa taaaaaaaca gttaaaattg
      121 atagataatg acctgtcaga ttttggaagc atatgtataa tagaaaaaga acattataca
      181 ataagtgtaa acagtatagg tgaaataaat gtgtattatg atttagagta cgaaaataag
      241 gtgtatagaa tagtttatga gattgaaaag ttatttaaat ctcaagttgg aaggtttagc
      301 atatctacat acagaaattg ataattaaaa aagtagaaat taaaaaactt aatactaaag
      361 atatagataa atatatgagg taacagctag agagag
//
LOCUS       DAMJYX010000279          363 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJYX010000279 DAMJYX010000000
VERSION     DAMJYX010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.286"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="PC479_003673"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="PC479_003673"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="PC479_003674"
     tRNA            290..361
                     /locus_tag="PC479_003674"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAMJYX010000280          335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMJYX010000280 DAMJYX010000000
VERSION     DAMJYX010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.287"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
BASE COUNT          130 a           34 c           28 g          143 t
ORIGIN      
        1 attaatgaac ttaaaattaa tatggataaa tattctaaac ccagtattta ttaaaaatga
       61 aataaaaaga aaaaaaatac aaattattat ctttttcttt ataaactttg gtttaactta
      121 ttcattaggt ttactgttat attataataa atttattgat ccagaaaact ttgcatcatt
      181 catgatgata ttacctctat cttcagttgc cattgcaaag ttttatactg aaggattaaa
      241 taatgatatg tatgaatttt attcactaat catattattt tttcttattt attcattatt
      301 aattgttctt aagttattaa atctaataaa taata
//
LOCUS       DAMJYX010000281          304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMJYX010000281 DAMJYX010000000
VERSION     DAMJYX010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.288"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
BASE COUNT          138 a           28 c           32 g          106 t
ORIGIN      
        1 tgattttatt aaaaaaagag atatacaaac taataagttg taatctctca aatataaaaa
       61 aacaataaat tagctcattg gattttttag tccataatat aattatagat tattcaccaa
      121 acaatctata gaaattgtgg tattaatgtt taaataacca actttttcta aaataacctt
      181 tataggaata tatgagttca taagtaaaga tagcgaaata agaaaccagt ttggtatgta
      241 aaatctagat ttttaattaa attatcaaga ttatatatta tagtatcaaa aatagaaaat
      301 taaa
//
LOCUS       DAMJYX010000282          232 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMJYX010000282 DAMJYX010000000
VERSION     DAMJYX010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.denovo.290"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..>232)
                     /locus_tag="PC479_003675"
     CDS             complement(<1..>232)
                     /locus_tag="PC479_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2806143.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAMJYX010000283        18275 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.guided.070, whole genome shotgun sequence.
ACCESSION   DAMJYX010000283 DAMJYX010000000
VERSION     DAMJYX010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.guided.070"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            complement(<1..658)
                     /gene="bclA1"
                     /locus_tag="PC479_003676"
     CDS             complement(<1..658)
                     /gene="bclA1"
                     /locus_tag="PC479_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2806144.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     A"
     gene            complement(1336..1860)
                     /locus_tag="PC479_003677"
     CDS             complement(1336..1860)
                     /locus_tag="PC479_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2806145.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(1925..2677)
                     /locus_tag="PC479_003678"
     CDS             complement(1925..2677)
                     /locus_tag="PC479_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2806146.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(2708..4006)
                     /locus_tag="PC479_003679"
     CDS             complement(2708..4006)
                     /locus_tag="PC479_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2806147.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(4023..6287)
                     /locus_tag="PC479_003680"
     CDS             complement(4023..6287)
                     /locus_tag="PC479_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2806148.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(6542..7360)
                     /locus_tag="PC479_003681"
     CDS             complement(6542..7360)
                     /locus_tag="PC479_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2806149.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(7353..8051)
                     /gene="cbiQ"
                     /locus_tag="PC479_003682"
     CDS             complement(7353..8051)
                     /gene="cbiQ"
                     /locus_tag="PC479_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2806150.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(8054..8338)
                     /locus_tag="PC479_003683"
     CDS             complement(8054..8338)
                     /locus_tag="PC479_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2806151.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(8328..9080)
                     /locus_tag="PC479_003684"
     CDS             complement(8328..9080)
                     /locus_tag="PC479_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2806152.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(9262..9454)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9586..11358)
                     /locus_tag="PC479_003685"
     CDS             complement(9586..11358)
                     /locus_tag="PC479_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2806153.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(11372..12301)
                     /locus_tag="PC479_003686"
     CDS             complement(11372..12301)
                     /locus_tag="PC479_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2806154.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            12522..12707
                     /locus_tag="PC479_003687"
     CDS             12522..12707
                     /locus_tag="PC479_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2806155.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            12700..13401
                     /locus_tag="PC479_003688"
     CDS             12700..13401
                     /locus_tag="PC479_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2806156.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            13403..14326
                     /locus_tag="PC479_003689"
     CDS             13403..14326
                     /locus_tag="PC479_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2806157.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            14483..15406
                     /locus_tag="PC479_003690"
     CDS             14483..15406
                     /locus_tag="PC479_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2806158.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            15399..16136
                     /locus_tag="PC479_003691"
     CDS             15399..16136
                     /locus_tag="PC479_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806159.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            16147..16920
                     /locus_tag="PC479_003692"
     CDS             16147..16920
                     /locus_tag="PC479_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806160.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(17461..18069)
                     /locus_tag="PC479_003693"
     CDS             complement(17461..18069)
                     /locus_tag="PC479_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2806161.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5928 a         3133 c         2271 g         6943 t
ORIGIN      
        1 tcgctcctgt acttccggtt gggcctgtta ctcccgttgg tcctgtactt cctgtcgctc
       61 ctcttggtcc cattggacct gtaattctaa ttggaagaca ctcacattta ggataacaat
      121 tatctataat tctatcttca aatgttacaa gtatatacgg tttgttacaa cctctactag
      181 atgtaaattc aagaatctga taagggtaat ttaaagataa agctaaccca taattaggat
      241 atttattcat tgcccatgct tttatagttc ccgtaatatt taaacaaata tagttggatg
      301 tatcacttat cccaactggt aaatatcccc taatattttc catagacact ctatcattcc
      361 atgttatagt tcgtaaatta taatattcac ttattctttt aatttcaata tctacttttt
      421 ctttaaaaat atgattctta tttgaatcaa tatgaataaa taactctgct tttaatatac
      481 ttttgtcttt taatatatct ggtattctca ctttaaaaaa agtaatcaac ttttctttta
      541 caaaactatt tgaacattta cttcctatta agcaataatc taaattacta aagtttttat
      601 ctggatattt tttagatata aaagtatcat catttaaata aagtataata tttctcattt
      661 ataatccctc cattttgggt ctatatcata ctatgtttaa aatacatgct tagttacaat
      721 ataattaaaa tttatagtat taaattttta tataataatg ctgatttcta caaaaaaaga
      781 acatgtcatt agaaatttct aatagcatgt tctttttcat tattatatat tacactttaa
      841 attattcctt gactggctta gctggtgtaa actaaaccag tctttctaaa atattaagca
      901 cttttttctt attatgtttt ttaggattta ttgtctatat tattgtaaac caaagttata
      961 aaattgtaaa tagaatatat taccataaca atatcttttt ataagtaaaa attataaaaa
     1021 accttatttt tattaaatag aataaataaa ataagtttaa aaaatactga aataagtgtt
     1081 ttccacatat tttatatatt tatttaatac attattattt aaaaataaat atataaacaa
     1141 tcataataaa atttttttaa taaaacttta ttttttagtt aaaatcatac aatatataga
     1201 tttccacaat acaaataaat taaaaagtga aatgtatttg tctaaaaaca taataacatt
     1261 tcactttatc ctcattttta agtaatatat atttttaatt tttatttata actaaatcta
     1321 gtttttatat aaattttagt ttttttctac atctgagtaa tctattccaa aagtttctac
     1381 agtaacagat ttcataactt gtggagtttg tggcttatca gctgaatctg ttctaactga
     1441 tgctatttca tcaactgtat ccataccttc tatcactcta ccaaaacctg catactgacc
     1501 atctaagtgt ggtgagtttt tatgcattat gaaaaattga gaaccagctg aattaggtgc
     1561 catagttcta gccattgata tgactcctct ttcatgtttt aaattgttag taaatccatt
     1621 accactaaat tcacctttta ttgagtgacc tggaccaccc attcctgtac catttgggca
     1681 tcctccttgt atcataaatc ctggtattac tctatgaaat attattccat tgtaatatcc
     1741 ttcatttact aatgatacaa agtttttaac tgtatttgga gctatatttg gatataactc
     1801 aattttgatt tcttttccat tttccatttc tatagttact ataggatttt tattttccat
     1861 aatttattac cttccttttc attagttaaa ttacataatt acatttatta tattatactc
     1921 taaattaaag tctatcaagt atattttttc ctatttcata tccttcttga taaatagttt
     1981 tacaaaactc atcatcaaag ttaagcgtgt ctttaggagc aaattgagta ttaggtctaa
     2041 ctactattat tttatcttca tcatatactt ttttaatatc ttctggcaac tcataattat
     2101 attttgtgga tattaaaata attttatcca cattatcccc aattaatggt tcaattaatg
     2161 cactcctaat taaacctcca tctaatttga aaccatcata aattccttct tgcacatctt
     2221 tcacaaattg ttctttaaat ccaagagttg cgtttggatt tgatggtaac aacccactgt
     2281 atgaaatgct gataatggct tcattacgag gcatttttga tacgtcaaca atcttttctg
     2341 atactacttt attatcaact tcaacataat taacataaaa gttcatttct tctatattac
     2401 tatcatctag ttcccttagg ttgtctatag caggagaatt atccacagta ttatttacta
     2461 tttttacatt tccattctgg atatctttta tatttgtcca cattttctcc aagttatcaa
     2521 cattccctgt gtacaaataa tatccattta ttgctcctat tgatgtccct gaaatcgaat
     2581 aaaatttatt tattcctcca tcatacaagg ctttaactac tcctgcttga taagcccctt
     2641 ttgctcctcc accttctaaa cataatccta atttcatagt ctcatctcca aattgtttat
     2701 cttatattta tatttcaaaa aacttactaa atgttttata tatactatag tggtcctcaa
     2761 catttcctaa ttctcttata gaatgcattg ctaaaattgg acttcccaca tccacagatg
     2821 gtatatctat atgagtagaa gatattggac ctatagtact tcctcctctt tcatctgacc
     2881 tattaacaaa ttcttgatac tcaacattag cttctctaca tatattttta taaatagcta
     2941 tagaatatcc atcactagta tatgcctgac tagcatttat ctttataacc ggaccttttc
     3001 ccattattgg tttattagtt gggtcatgct tttctggaat gtttggatga atagcatgag
     3061 ctaaatctga tgatatcata aaagatgaat aaatagactc aaagaatccc tctctatcct
     3121 tacccattga tatgcaaatt ctctccaata tatttaacaa catgtttgaa tctgcacctt
     3181 gtttagtaga gctaccaact tcttcattgt caaatacagc cattacattt acaccatgtt
     3241 gacctttagt attaacaaga gcattcaaac tagcatgtac cattgcaaga ttatcttgtc
     3301 tcccaataga tataaattca tcattagccc ctattaaact tcctttttca aattcatata
     3361 aaaataaatc aaaatctata atatcgtcca tatttacatc taagttagat gctatttgct
     3421 ttataagaaa attatctttt tctaatgtat catttaacat cccaactaaa ggtaacatat
     3481 ctttttgctt attatatgag tgaccatcat ttatacttct atttaaatgt attgctagat
     3541 ttggtattat acaaattggc tttttaaagt ctataagcct ttcatttggt tttaatatac
     3601 tatcaccttt taaaactact ctaccagcta tcccaagagg tctatccaac catgtactta
     3661 atattgctcc tccatagcat tctgtattta acttcaaata agatttttca gcttctattt
     3721 ctgcatttgg tttaatacta aatgtaggag aatctgaatg agagcctatt attctaaatc
     3781 catctctttc tataccttct gaatttacta caaaagctac tatagctgat aaattcttag
     3841 taacataata ctttccacca actcttagat tccatctttg attcaaaact aattcttcaa
     3901 atccattttt atctaagatt tccttagtac tctcgactgc atgaaaagac gacggactat
     3961 catatatgaa gtctattaag tttttagcaa agtttatact atccattcct catttacctc
     4021 cattataaaa tatttagtgt tgtatattct ttatttattt ttttaattcc ctgttggtca
     4081 aaagctatag taacatctgt accaattata gaaaccactg tcccaacccc aaatttagga
     4141 tgatgtacct tagaaccaag ttttatatca tctggattag tttctttatt agaatctttt
     4201 attgtagcat taactttatc tgcaacttta gatttgctta tagtactcat atacttttta
     4261 gtatatttat ctaacatatt ataacttgct ttagaatagc ttagttcttt ttttactttg
     4321 tacaatcttt ctatacattc ttctggcagt tcttccataa atcttgaagc tattgcaaca
     4381 tttgttttac catataatgt tctcttctct gtcaatgaca tatatagctc ttctttagct
     4441 ctagttatac caacataaca aagtcttctc tcttcttcta tttgtgaatc actcatagat
     4501 tttatagctc ttgatatagg aaataaccct tcttccatac ctgttaaaaa cacaacaggg
     4561 aactctaaac ctttggatgt atgaattgtc ataagcgaaa ccctatcatt gtcttcttca
     4621 tcatttgatt cagatgtaag tgctatactt gttaggaatg tctctaagtc tttattttca
     4681 taagtatcat cattactact ttcaaactcc aaagctatag aaataaattc tctcaaatta
     4741 tcaattctgt cttgagcttc ttcacctttg cctgttaaat ctttttcatt tttatttctt
     4801 atctctacaa gctcgtccat atatcctgta gtatccaaga ctttttctat aagtttgcta
     4861 acaggataaa cttcttttat agttctaaga gtccctataa tatcaacaaa tccacttata
     4921 cttgcccttg ccttagtaga tatatctgaa tttgtttcta tatcaattaa tactgagtat
     4981 atactttcct gttttaagtt agctctatct tcaatttttt ctatagttct aagccctata
     5041 cctcttctag gaacatttat aatccttttt atagatatat catcttgtgg attttgaata
     5101 actcttaaat acgccaacaa atcctttatt tcttttctct catagaattt tgtaccacca
     5161 tatatattgt atggaatttg acttctattt aaagcgtcct ctacaggtct tgcctgtgca
     5221 tttgctctat aaagtactgc aaaatcctta taaggtctat tttcttttct agctatttgt
     5281 gcaattgaat cagcaatgaa atcagcctct tctatttcac taccagttaa ttgtatcttt
     5341 ataagttctc cttctttttt ttcactccaa agcttttttc tttttctttc tatattatta
     5401 gatataactt tattagctgc atctaatata acttgtgtag acctataatt ttgttccaac
     5461 tttacaacat gtacattgtc ataatctttc tcaaattcaa gtatgtttct tatatctgca
     5521 cctctccatc cataaatact ttggtcatca tctccaacaa cacaaatatt ttgatgttct
     5581 cttgctaata attttatcaa ttcatattga gctttacttg tatcttggta ctcatctacc
     5641 attatgtatc taaatctact tctgtaatat gctaacactt catcattagc cttgaaaagt
     5701 tctacagttt taagtattaa atcatcaaag tcaagtgcac tatttctttt taaacggtct
     5761 tgatatagcg cataaatatc tgcaatctta accattctat tatctgccat attgatatct
     5821 ttaaactgct ttggagtata aagtttgtct tttgctcctg aaattgcact tataactgct
     5881 tttggttcaa acactttatc acttaaattt aattctttta gacagtcttt aaccaatgta
     5941 acttggtctg ctgaatcata aattacaaaa cttctattgt aaccaatctt atttatatct
     6001 tttcttagta tacgaacaca gcaagaatgg aatgtactta tccacatatc cttagtttca
     6061 ggacctatat tttgttcaac tctctctctc atttcatttg cagctttgtt tgtaaatgtt
     6121 atagctaata tatttggtgc ttgtacacct ttatcttcta taagatatgc tattcttgtt
     6181 gttaatacct tagttttacc tgaaccagca ccagccagta taagtactgg accttcagtc
     6241 ttttctactg cttctctttg agcaggattt aatgtatcta aattcatatt tctcctcctg
     6301 ttaacagcca taaatatatt ttaatctaaa atatgaatta cttcaaatta taattatgta
     6361 ttatattaat agattatttc caatatatta caattaaata acatttttcc atataattta
     6421 tttcattgtt taataaaaaa aataacctta tgatttgggg aatcataagg cacctttaag
     6481 gggtctaggt taagggttat tatatataaa tctatttttt taaaataggt ttagggctta
     6541 attatttatt tgtattaagt tctctttcct tccaatactt atacaaatct tcttctttct
     6601 taaataaggg taaattcata tttctatgta cttttactaa ccatggggat tctaggccag
     6661 cttgtataat attattttca tttataaaca cattttctgc ttttccttca tcaatcaaaa
     6721 tccccttatc caatacatat atgtaatcac atatttcata cattaaattc atatcatgac
     6781 tggatattac tatttttata ttatttttat ttaatccctt tattatatct acaatagacc
     6841 tagtacttac tgggtctaaa ccagctgtag gttcatcaag taatacaatt tcattttcca
     6901 ttgctataac tgatgctatt gccactcttt ttttctgtcc atagctaaga aaatgtactg
     6961 gcctgtctat aaaatctatt ccattaacag ctactagagc tttgtttatt ctctctttaa
     7021 ttgttttttc atccatacca atgtttctca ttgcaaaggc tatatcatca tatacttttg
     7081 aataaaaaat ttgtttttct gggtcttgga agacaatacc tacatttttt ctaaggttgt
     7141 ataaacttct cttatcatat tttaactttt cttctttaaa aagaatttct ccagatgtag
     7201 gctttaatat acccattaaa ttcataaata atgttgattt tcctgaacca tttgaaccta
     7261 ttatacctat aacatttccc tttgaaaaat ccatattaat gtttaatagt gcattggtat
     7321 ttttttcata ttgatacgtt aaattattta ttttaaacat cattatcccc tactatgtga
     7381 aatttaccat catacaattt tatgtctaat gatatacaca tatcatcata ccttgtcata
     7441 agtcttttgt acagcatact tccaagaata cctaaggatt tatatgaagt ttttaaattt
     7501 atatatccaa atcttagctc ttgtgacttg tatatttctg aaaattcttc taaaaatatg
     7561 aatataaacc tatatattaa cattgataac tcgacaaaag tgtcaggtaa atgaagtttt
     7621 ttaaaaaaga atattaattg attaaaaggt gttgtaagta tacaaaaata cacacaagtc
     7681 aaacaagaca tcgctctaaa taacacatga atagacatgt taatagacgc ctttgatatt
     7741 cctacattaa aactaaaaaa ttcaaaacta tataataaat ctgctttatt aaaagatata
     7801 tttacaagag ttattcctat actaagaaat aaaaagtaca ttggtattct aagtaatttt
     7861 aaataatttt ttaaatctat tccagccaca caaacaacca aaatactcat taaaatcata
     7921 attccactta aaataaatat attttgtatt accatagaag caatcagaaa aattactcct
     7981 atggctactt ttttgtttgg atttacttta ctcaatctat ttgtatatgc atatttatca
     8041 attataagca tgattaactt ctctttcctt tttgaagacc taatatgtat cctattacac
     8101 cagcccctaa agctgcttgt gcagagaata ataaactttc tatttctcca cttggtggct
     8161 catatggact gctaaaccat ggtttgtaat ttggatttat cttagtaatt tcactttcag
     8221 cttgaccatc tgctcctcca tattcagcac ctgagttgac aagtaatggg aatataataa
     8281 gagctactac taataaaagt aataatatgt tagtttttgt ttttgtttta gcactcatta
     8341 gctttaactc ctttatcatt attttctagt atgctttttt ctccgttttc tgtaattaaa
     8401 ttatatacaa ttacagtaag tataccttct gctattgcta ttggtatttg agtcataaag
     8461 aaaattccta aaaatttaat tgctgaagcc attaccccac ctgatgggtc tgggaaagcc
     8521 agtgctaatt gtattgaagt tattgtatat gtggctaagt ctcctattgc tgctgcaaga
     8581 aatactggaa tagcattatt tccatctttt ttctttaaag ccttaaatat taggaatgat
     8641 acgattggcc ctattatcgc cattgaaaaa gcattagctc ctaaagtagt tattcctcca
     8701 tgtgctaata acaatgcttg gaatattaaa actatagttc caagaacaaa cattacgctt
     8761 ggtccaaaca ttattgctcc aagacctaca ccagtaggat gtgaacaact tcctgttact
     8821 gaaggtattt ttagtgctga taatacaaac acaaatgctc cacataaagc taataatact
     8881 ttctttttag ggtcttgctt tactatttta cctattgatt ttaatccgaa taagaaaaat
     8941 ggtataaata taactcccca tgctatagac cattttactg gtaagtaacc ttccattata
     9001 tgcattgcat ttgatgtaac tggagttaaa atgattaaca taagtgcagc tataacccct
     9061 aatttaatat tctgtttcat cttagtatcc ctctctccct ttctaataat taaaacataa
     9121 taatacatgt ctgatttatt taataaaata aaaaagccta actcgatggt taggcttcta
     9181 attttccaca atctaaatac aaacacataa aacaaacata tctgtataat tattataaga
     9241 aactttctcc atccccgaag aaattacttt aatattttag gcaggtctcc tgacttagct
     9301 tcatcctact ccttttcctt cccatgagat ataaaaaata tcatcatagt ggctaacaaa
     9361 agtttcgtct gcatcacagt agcgggggct gtaaaggaat ttaacctttt tccctattaa
     9421 tcttgcttaa agaacctata atattatata tttaattttt aaattattct taaatcattt
     9481 tctatataaa tcattgtata ccttttctgt gaaaatttct acatttttta taaattttat
     9541 tcatttttat atttttatgt atataaattc ttttttgtaa gtttattaat ttctaattat
     9601 cttctttaaa tacattgaaa ctacatcaga aaatctagtt atatctcttg aatatataaa
     9661 atcatttcca tatattaatc tttcagcttc aagttctgat tcttttcctg taaatactcc
     9721 taatactaat atattttgct ttctagcttt tctcacttca ttggctgtat ctctcaaacc
     9781 aactataccc ctataagcct tttctcctct taatgttctc tcactacttt ttcctatctt
     9841 tacatcattt ggtcttccat cgctgagaac tatcaggatt ttattttctt catctctatt
     9901 tattagacca ctacatatag attttattgc caaaccatct ctattattac cagcacaaaa
     9961 atattcaaat atattttcac tattttttat actatcatca taatctttaa atctttttaa
    10021 tattgtatag tctaaaaaac tagaaaatcc catcactcta tttggtatgc caacttctgt
    10081 tagagcattt gcaattatgt aagcttgaat tgccacattt ccctgatttc tactttgaga
    10141 gccacttgca tccaataata tatcaataac gtatttccct ttttcatttt ctatatcttt
    10201 ataaaatatt ttattattat tacttctacc aactctccaa gctctattgg ctaatatagt
    10261 accacaatca gaatatactc tacttttagc attttcttct attaaaattc ttgacagact
    10321 ttcttttaat ctctttattt gctgtctata cattctagca tgttctctaa attttcctaa
    10381 attattttct ttttgtcttg ttacatattt tagttgaaat acattattac actcacttct
    10441 aagaactcca tctgtaaagt gtattctaca accttcatga acatttctac aattttgtgt
    10501 ctcaattttt ttaatttcac ctttactcaa ataagttttt ccataataat gttctatttt
    10561 tgaatatatc ttatttgcag tgtcttcatc aacatatata actcggtctg ctgaattgct
    10621 acatttagaa tcatcaaaat cccctacacc ttctacaagc atggatgaag atattgaatc
    10681 tatatcactt tcaataatat tttcttcttc atcactataa agttcttcat acatgaaatc
    10741 agcaaatgta tcaaaattta cttctacact ttctacatta ttttcatcaa taagactatt
    10801 ttcttctcca tttaatatgt attcaaaata agttaaatat ataacatcta atgcttttaa
    10861 aatctccata atatcttgat tggtatctat atttcttata tcattaacta ttcttctagt
    10921 catactatct aatgctgggt gcttgtccat actataaagt acaaatgtgt atttaatttt
    10981 atccataata ttatcattgt gtattttggt aaaacgtgaa acaatatctt tataagcatt
    11041 ccttcttatg tcttctatcc ctggcctgtc tttaatgacc ttttcttcaa ctattgtatt
    11101 taatataact tgaattagac ctagtataac atctttgttg tatccttttc ttattctatt
    11161 tgtaagatat tttttaacta tatcccaatc tacatacttt cttctagcac ctgcaattat
    11221 tgcgaaatat agacctattt cttttgaaga gtaatctttt tctgatatat ctatactttc
    11281 attgtaatca ccagctattg tccatgatag gttattagct ctattttcaa attcataatc
    11341 atcatatatc catttcataa tctcacctat attaaatatg acttattgga aacacatcta
    11401 tactctccca tttattaggt attctagttt taattacatc tccaaccatt tctttctcat
    11461 atacatcaaa agtctttcct gtaagtccca tattaattgc aagagttggt tttaatcctc
    11521 ttcttatagt cttaagagat gccataagac cacgtaaatc aatagcttta ctagatatct
    11581 ctccattttg agacttcaat tgtaaatcta aaaatattcc tgcaaagtgt attaattttt
    11641 cttcatcagc atctgaaaat tcatttttta aaatcatcat taacttatct tcttctatag
    11701 gaggaatatc tattaccata aatctagata ccagtgcttc attaagttct tttgttcctg
    11761 catattcata attcatagtc ccaataaatc ttgttgctgg atgtagattg actctctcat
    11821 acccaggtac atcaattatt cttctatagt ctaaagctga atgtaccaca actatagcat
    11881 cattttttgc catattaatt tcatcaaaca ccccaaaacc tccattaata gcacactcat
    11941 aaactgagcc tcttctaagt ttaacttcat tgtctataaa tgtatctgta cctataagtg
    12001 ttgaactgtc tgtatttata tgaaatgatg tattccactg aggtctatta aatatttccc
    12061 ctagattatc agcaagtaag ttttttcctg ttgcctttgg tcccgataat aatatatttt
    12121 cttcttctaa aatagctgct atacacatag acaagatatc tttaccataa aaataagttt
    12181 tagactttgt tactctgtat tctaaatctt tttctaaacc ataataattt ctaaaataca
    12241 gtacatcttc aataagtttt tcatctaccc cttgcaccct tagattatct attatatcca
    12301 tatttttacc ccttttatat aaatagtatt tgtttcattt cttatcacca ttttagcata
    12361 aaaggatata agctaaaatt gattttttaa tctccccatt ttaagtaaaa ccatgcacca
    12421 ttttatcact acaaaattaa gagtactact tactgatatt tcttgatttt gttcgtctat
    12481 acgtatataa tatatgctga taaattatga ggaaaggatg attgaaatat ggatgcccag
    12541 aagtagcaaa aaaatggggt atttctgaaa gaagagtaca aaaactatgt gaagaaagtc
    12601 gcataccagg agtttcaaaa ctcgggtata tgtggctgat accaaagaac gcagaaaagc
    12661 ctgttgatgg tagaacaaaa agaagaaagg aagtacaata tgaatagcgt tttgattata
    12721 gatgatgaca aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct
    12781 gctattactg cacattgtgg tgcagaaggt ctacatttgg tcgataaaaa tagacacaca
    12841 ctctctcttg taattttgga cataatgatg ccagatatag acggttttca agttctgaaa
    12901 aaaattagag aaacaagtaa tattccagta attatgctta ctgctaaaaa tgacgaggat
    12961 gataaagttt ccggactacg tttaggtgct gacgattacc taacaaaacc gttcagcata
    13021 aacgaactta tggctcgtgt caattctctt attcgtcggt atactatgtt aaaccctgct
    13081 tttataacag acacagattg cataataata aatggcatga ttattgacaa gacaaaccga
    13141 attgtttctg tcaataatat tcctgttcaa cttacaagca aagaatttga tttacttgct
    13201 tttctggctt ctaataaggg acgggtgttt accaaaaaac agatttatac acaagtatgg
    13261 gaagaagaat atgcttttga cgatagtaac atcatgtctt tcatcagtaa attgagaaaa
    13321 aagattgaac cagaccccaa ccatccattt tatattttga cagttcgtgg tgtcggttat
    13381 cgttttaata aggaggctta acatgaatac aagtgtctat ctgcttttga tattggttct
    13441 tagcctgatt attatagcgt accttgtttc tgtcctgcta cgtgtccgaa tacaacttac
    13501 acttataaaa gatgctttag aggatataaa aaatggaaac cttaatcgac gtattttaac
    13561 agatgaaaac gacattacaa aacaaatttg ctatgatatt aacgaaatag caatcaatag
    13621 ccaatttcaa cttattcagc aaaaacaatc agaacaagcc tacaaacagc taatgacaag
    13681 tctctcccat gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg aagcagtaga
    13741 aagtaaaatt gttgtaggag aggaaaaaga cgagtatatc catgttgcgt ctaataaggc
    13801 tcattactta aagaattttg tagaaaatct atttgaatgg gtaaaattgg attctaagga
    13861 acaagttttt cattttgata tttttgactt gaatgaatta tcccgtaata ttatatctga
    13921 ttggatacct gttttagaaa gcagtcattt tgaatatgaa tttgatatac cagaaataga
    13981 gtacttctta cgtatagacg caaatgcata tacccgtatc attaataacc tattacaaaa
    14041 tattattacc catagtagtg gcaacaaaat gacattgaga atttttgaaa acaaagagca
    14101 agctcaaatt ataataacag ataatgggaa aggtatatct tcagacaatc tcccacatat
    14161 ttttgaaagg ttgtaccaat gcgaccattc acgagcttca aagggaaatg gtttaggctt
    14221 ggcgattgca aaggaactta ttaacgctca taagggaaat attaccgcca acagcactcc
    14281 cggtatggga acagaattta caatattgct tcctaaagcc ctgtaaaagt acgaggcttt
    14341 cttactctaa aacaagataa aagcaagatt atggcaaggt tttggcaagg ttcatgtttt
    14401 ataattctaa ttgtagttga ttttgacgtt gtatcaacta tcttttgtag aaacaaattt
    14461 attaaaagtg aggaaattat atatgaatga ttttgtgatt gaaacaaagc aacttacaaa
    14521 aatttatggg aatcaaacag tagttgacaa agtcaatctc catgcaaaaa aaggccgaat
    14581 atatggtctg ttaggacgca acggagctgg gaaaaccaca attatgaaaa tgattttagg
    14641 attgacttca atcacgtatg gcgaggtaga tgtatttgga gagaatatta agggacgtga
    14701 aaaacaagta tatccccgta tcggtgcaat tatcgaaacg ccaggatttt atcctaatct
    14761 gacaggaacg gagaacttgg agatttttgc aaaactgcgt ggtacagccg cccctaattc
    14821 tgtaaaaaga gcattggaaa ttgtaggctt accttataaa aataaaaagc tgtttagtga
    14881 atattcactt ggcatgaaac agcgacttgg tattgccaac gctatcctgc atgacccaga
    14941 actgttagtt ttagatgagc caaccaatgg tcttgaccct attggtattg cggagatgag
    15001 aaagtttatt agggacttga gcgttgagca tggaaagaca attcttattt ccagtcatat
    15061 tctttcagaa atctcattgt tggctgatga tattggaatt atcgaccacg gtgttctttt
    15121 ggaagaaagt agtatgagtg aactagaaaa gaaaaacagt aaatatatcc tgctacaagt
    15181 ttcagatatt ccgaagacat cacttatttt agagcgtcaa tttaacacga aggattattc
    15241 cgttcaagat gactacacgt tacaccttta taacactaat ctggatatgg gtgaaatcaa
    15301 taaagcactt gtaatgcaga atgtaactgt tattagttct caactttgta atgatacttt
    15361 agaggactac tttaaaaaaa tcacaggagg agagggcatt gcttaaactt attcaaacag
    15421 aatttcttaa actgcgtcgt aggaaactta tttggctgat gttgttgtct gctctcatta
    15481 tgccgttttt cgcactacta tacttcaatt attttggtaa aacaggtgtt gagccaataa
    15541 agttttataa atggtctgca tttggttata cactttggat tattttgcct gttgttttgg
    15601 gtatattttg caccatgctt atacacgaag aaaaccagta tgatatgctc aaacagcttt
    15661 ggattgtacc tattagtaaa atgggatact tttttagtaa atttttcgtt gttttgattt
    15721 actctatttg ttttatgctg attacagccg ttgcttctgt gttatttagt gtactttctg
    15781 gatatgtcgt atttagctgg gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg
    15841 gtgtaatcac tgcttttgta atgttgccga ttttggcaat cgctacatct caaaaaggat
    15901 acattctccc aatttgtatc acattggtat atgccttttc cggttttgtt ataatgacaa
    15961 taaatatgta tttacaccca ttatccagta tgggagttat tatcatgaga aataaagaca
    16021 ttccaggtct tatattttca caggaaatta atgtcccatt agcatttctt tgtatttgta
    16081 tttggagtat tgtatcagta ttgtttgcaa acactgcact aggaagaaga aagtgaggtg
    16141 tagataatgt ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt
    16201 tggattacag tttttgcatc cattatgata gcagtcatag tttttgcagg agggcaggat
    16261 gtatataatg acccagattt acattatggc ttaaagagcg tttatgacgg ttcacggtat
    16321 atagacaatg caggttggta tatggacgca gtacaacctt ggtcaacttt ttttgttctt
    16381 cctgctgtca ttgccctatt aggaagctat attatttgtc gtgaagaaga ggaggacact
    16441 attaaatccc tgcgtcttat tcccataaat gaaacaaaat taaccatagc aaaaatgatt
    16501 attactttta tattcagtat acttctttat ttgttactct ttactataac atttttgact
    16561 gaatccgttt tacatttttc cgatttatcg acaaaactgg ttttaagttg catgaaagag
    16621 tattttcttg atggcatagg tgtattcttt gcaatttctc ccattattgc cttggtatcg
    16681 cgcatgaaaa aaggatattg gcttgcatta gtattcacag aagtttattc tattgctgga
    16741 ctatttgcgg gtatgtcaaa tgtcttacaa accttttttc ctattgcggc aatatttaat
    16801 atttctggat actatataac tactggagaa aaaattatgg ggagtatcat aagtttatta
    16861 ctttgtgcct gcctttctgc atttatactt aaagggctaa aacacaatga aaaaaattga
    16921 aataggtata tcaaaatgaa aaagataaaa aaactgatta ttctaattgt attcttgttt
    16981 ctttttggca tgttacactg ttatactgaa tggaaattca acaataaccc acctaaaggg
    17041 gtaatagaaa gaataaatga atggactgat aagattggac tggattagta acttatgtta
    17101 attatagtga atgtaatatt agcaagttat tgtttcgtat tttaatacta taactttaaa
    17161 tatttggttt taatcttcca aacgaatata aaaaaactgt cacagaggca acatattttt
    17221 gaatatttat aaagtggtag ttttgatttt caaagctgcc gccttttctt ctatataagc
    17281 aaacttatcc tcaacaaagt gtaaaacatg gtacatttta agaaaaaagt accacagaca
    17341 caagcaataa aagaaacgcc gagaacctac aaaaacgtag tgcttatgca gatttacgac
    17401 gccttctatt ttctcaaccg tcttaatgac cttgtacaaa agaaattttt ctaatatttt
    17461 ttataatctt atcttgtgat gttttttatg tataaatgac ttaataacaa atccaataaa
    17521 aattatggat attggtataa ttagaacgag gaatttataa tcattataat gtgacattat
    17581 tcttacccaa ctctcagaaa aaatatatcc tatactgatt aatgaagtat tccatatggt
    17641 tattcccacg atagatgata ttacaaaagt tataatattt tgacctgcta tacctgctat
    17701 aaaagatatg tatgttctac ataaagggat taatctacat acacaaacag aaatagaaga
    17761 gtatttgtta aaataatctt gagatgcaaa tattcctttc tttgcctttg gacatatctc
    17821 tattatttta tttatgatag atttacctcc ccaatttccc actgtataac aaaatatcga
    17881 accaagtata cctgcaatta tacttaaaat taaaattgtc agaaatccaa aatgatttct
    17941 tgcagcaatt gctccacata aaggtaaaac aacttcgcta ggtaatggaa aacaagcata
    18001 ctcaatcata attaatacaa atacagagat taatccatat tgtaaaataa aactattgat
    18061 tacagacact tacttatcta tctcctttct tatttaaagg ttttaagggt acatgttcta
    18121 aggattctca ccctatattt aaagaatcaa tctctttaaa ctattatatg tatttacaca
    18181 aagcatttat aacatacttt aaaaatagta ccttaaaata agaaacttaa taaataaaaa
    18241 aagattacgt ggctacgtcc tactctccca ggcag
//
LOCUS       DAMJYX010000284        14951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.guided.083, whole genome shotgun sequence.
ACCESSION   DAMJYX010000284 DAMJYX010000000
VERSION     DAMJYX010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.guided.083"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            30..983
                     /gene="blaCDD"
                     /locus_tag="PC479_003694"
     CDS             30..983
                     /gene="blaCDD"
                     /locus_tag="PC479_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2806162.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1327..2010
                     /locus_tag="PC479_003695"
     CDS             1327..2010
                     /locus_tag="PC479_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2806163.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2007..4667
                     /locus_tag="PC479_003696"
     CDS             2007..4667
                     /locus_tag="PC479_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806164.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(4848..6029)
                     /locus_tag="PC479_003697"
     CDS             complement(4848..6029)
                     /locus_tag="PC479_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2806165.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6029..6691)
                     /locus_tag="PC479_003698"
     CDS             complement(6029..6691)
                     /locus_tag="PC479_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2806166.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(6823..7461)
                     /locus_tag="PC479_003699"
     CDS             complement(6823..7461)
                     /locus_tag="PC479_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2806167.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7640..8245
                     /locus_tag="PC479_003700"
     CDS             7640..8245
                     /locus_tag="PC479_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2806168.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            8749..9498
                     /locus_tag="PC479_003701"
     CDS             8749..9498
                     /locus_tag="PC479_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2806169.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9495..10259
                     /locus_tag="PC479_003702"
     CDS             9495..10259
                     /locus_tag="PC479_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2806170.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10358..11179
                     /locus_tag="PC479_003703"
     CDS             10358..11179
                     /locus_tag="PC479_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2806171.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11193..12857
                     /locus_tag="PC479_003704"
     CDS             11193..12857
                     /locus_tag="PC479_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2806172.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            12862..14736
                     /locus_tag="PC479_003705"
     CDS             12862..14736
                     /locus_tag="PC479_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2806173.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5613 a         1722 c         2489 g         5127 t
ORIGIN      
        1 aaatttaaat atgggagatg atataaatta tgaaaagaaa aaagaatttt atttggatag
       61 ctatcttatt ggttggtgta gttatggtaa tgtattatgt agggaaaaaa cataacgata
      121 taaatcaaaa aactgataag aattatataa aatctgaatt aaataagtct gaattaaata
      181 aatccaaaat tgaaaacaat aataaaaaca aaaaagaaaa aattaatatg gttgactata
      241 gtgattgttt tgaaggtatt agtggaggag ctatttttta taatactaaa aataaagaat
      301 ataatatata taacaaagaa ttaattgaaa caagaaggtc accatgttct acttttaaaa
      361 tagtttcaac tttaattggt ttagaaaaag gggtaataaa ctcaaaagaa tcagttatgg
      421 gatatgatgg tacagagtat ccaaataaaa attggaataa aaatcttagt ttagaagagg
      481 catttaagga gtcttgtgtt tggtactaca aaaaattgat agataaagtt gatgctaaat
      541 ctgtccaaaa cattttagat gatttaaagt atgggaattg cgatattagc gaatgggaag
      601 gagatttaaa aaatggaaag ggacatttaa atggattttg gttagaatct tcattacaaa
      661 tatctccaaa agaacaagtg caaacaatgg caaaaatatt tgagggagat acaaacttta
      721 aaaaagagca tattaatatt ttaagagata ttatgaaaat agatgtaaat gataaaaaca
      781 taaatgtata tggaaaaact ggaacaggat ttgatgaaaa aaataaacgt gtggatgcat
      841 ggtttgttgg tatgctagag cgtgaaggtg acacctatta ttttgctatt aaatcagatg
      901 attccaataa agaaattaca gggccaaagg taaaggaaat tgcaattaat ataattaaga
      961 aatattatag tgtaagggaa taaacttaat tttatttgta aatattattt acctatagtc
     1021 tgtaatattt attaaataat tttactaaat tgagtctata tagagcaaaa ctcctttagt
     1081 acataaaata cttgctattg attattaaat tgaataccta tagatttaaa gttaatacct
     1141 tttaaagact atgtaatata ttaaaagtat aaataaaata tattgcatag tttttgtttt
     1201 atattataat tttaaataat gtgcaaaaac taaacaatat ttttaatagt aatttatatt
     1261 tgtattcatg tcactataat gtcattttag gttgctaaaa tttaaccata aaggagatga
     1321 ttgtatatgg aaatattaaa atgtgaaaat ctcacaaaaa tatatggttc aaatcaaacc
     1381 agagtgacag cccttaataa tgtaaattta tcagttcaaa aaggtgattt tgtatccatt
     1441 gtaggagcat ctggttctgg caaatctaca ttgttacatc tgcttggggg agtagataga
     1501 cctacatctg gaaagattta tgttgaagat acagaaatat caagtttaaa ggaagaagct
     1561 cttgcagttt ttagacgtag aaaagtaggt cttatttatc aattttataa tcttatacct
     1621 acccttgatg ttagaaaaaa tatattatta cccatgttac ttgataaaag aaaggtggat
     1681 gaggatagat tctctgagat tgtatctata ttgggattat ctgatagatt aaaccatctt
     1741 ccaagtcaac tttcaggtgg acaacaacaa agggtatcta tagctagaag tttaatctat
     1801 agaccagcta ttttattagc agatgagcca actggtaatc ttgatagaaa aaattcagaa
     1861 gaaattgtag acttgttaaa cttatcaaat aaacgattta atcagacaat acttcttatt
     1921 actcatgatg agaaaatagc actagaggca aatcgtattg taactatgga agatggagtg
     1981 attgtctctg aaaaggtggt gaagaaatga atattcttca agaatacacc ttagactttg
     2041 tacgacgcaa taaacgtagt agtatagcta taatgttagc tatattactt actacaacca
     2101 tgatgtcttc tttgtgtgga ctgttgtata ccatgtggac agattttata aaactttcaa
     2161 ttgagaaaga aggtaactgg catggtgaat tgtttgacta tacttatgga agtgacttac
     2221 cattaattga aaatttttct tcagttgatg atgtaatggt aaaaggacaa tggtatgtag
     2281 gcaaaattaa tgatgagaaa agagattata taatttatag aaatgctaca tcagagtatt
     2341 ggaattctat gccagaaaag gatactataa tagagggtcg tcgaccaaat aatgctaatg
     2401 aaattgcatt atcaaaacaa tattttgaga ataatccaag tgtaaaaatt ggtgataaaa
     2461 ttactctccc aataggagat agaatcctaa atggaaagcc tcttgaaccc atagccccaa
     2521 aatcgaaagg ggaaagtttt aaaaaaattt cagaggctac attgacaata gtaggaaagg
     2581 tagatgtttc tacttcatca gtaatacctg catatacagc tcttggatat cttgataaaa
     2641 caagtataaa acctaatgat agtataactg tgtacttgcg atttaaaaat ataagagaca
     2701 cgtataagga acttccaaaa cttgctaaat cattgggatg gaagactaat gaatatggaa
     2761 aatacaatct taaatacaat agtaattatc ttttaaagat gttagtcttt tcaccagaac
     2821 agaaagcatc tatgagtagt atatcaaaat tttcaactcc tattatgtat cttacaatag
     2881 caatttttac agtagccgtg tttgtaatgg tgatttacaa cgcgttttca ctgtctgcaa
     2941 atgctagact tactcaatta gggatattat ctagtgttgg tgcatcacca aaacaaatta
     3001 aaaggtctgt agtgtttgaa ggctttttac tgacaattat accattacca ataggtttat
     3061 ttttaggatg gttattgtgt aataggctta tagtttatgt taattcagtt aattatcata
     3121 ctgatgttcc agaagtggta tttacttatg gtattcctgc ttttctgcca gcagttttac
     3181 ttacaatagt aacagtctgg atttctgcac ttataccagc acgaaaagta tcaaagatat
     3241 ctcctataga agcaatacga caaggagata gtgttaaaat caaaaagtcg cataaatatt
     3301 cattaggaaa gatatttgga atagaaggag aacttgcatc aaacgcacta cgtgctcgta
     3361 aaaaatctta tcgtactgca acaatatctt taacattatc ttttttatta cttacatgtt
     3421 ttttgcatat aattgcaaat caagaaggag caaaagctgt ttatggaagt gataattata
     3481 aggaccaaag agatatagca gtttatttgg aaaatagtga acctgtagag tctgctttca
     3541 ttgaaaaatt agataataca gaaggtgtaa aaagttcact atattatcaa aaaatgtctg
     3601 cttccacttg gttgactgaa aaagatgctt caaaagaact tgcgaaaaat ggaggattta
     3661 aaaatatagc ttcatctaaa aagtactctc ctatagaacg tgatggcaag tttcgtataa
     3721 tttcaaatat aattgggctt gatgataaga gttttacaga atattgtagg caaatttctg
     3781 tagacccaaa gttattttat gatacaaaaa atccaacctc aattgtatat aataaggagg
     3841 aagatattaa tcgtagtaca cgtagaaata aagtatatat agattattta aatctttcaa
     3901 taggagataa cattaaatta aatgaaaaag tatatgacga agataaaggg gattatactt
     3961 acaacatgaa agtgggagct attacaagca cacttcctaa aatagtagat actagttcaa
     4021 agtatacatt aatgcaaata gttccaatgt ctatggtgca ggaaatatct aaaaattatg
     4081 atgaagtaag taggttacgt tcaaataggc tgataggagt atttaaagta aataatcgtg
     4141 aggacattcc tagagtaaga gaaaaattag agtccattta taataaagac tatggttcag
     4201 gagattatag tattgaggat gttttagaaa gtgaaaaaca aaaggctagt ggaataaaga
     4261 ccatgaattt aatagtaggc tttattacag gtttattggc tatgattgga ttatcaaatg
     4321 tacttgctac agtgtcagga aatattagaa gtagacgaca agagtttgct atgttgcgtt
     4381 ctgttggatt atctccagaa ggcataaaaa agatgcttat tttggaaggt ttattcttag
     4441 gaattacacc attactactt agtattcctg tgcaaatagg aatagtgtat gcttttctac
     4501 gtataaatga aatatatttt atagagtatt taccatttgc acctatatct acaataatag
     4561 gttttactat attgatactt tttattgtaa tagcatcata tatgacagga tacaagcagt
     4621 tgaagaatga aaatatagta gaatctataa aaaatgaaac aatataaaca aaatctattt
     4681 gaacttattt aaaggtttcg atttaagact acttacaagt atagggatag ttaagttgtt
     4741 tgtttaataa aaccttaaat gtaaatagaa gtatattact aaaacaatag ataacataga
     4801 atatagatat agctggatta taggtatttt acctataatc cagcatttta tgtgtaaaat
     4861 ttaattatga atcttgcacc accattatgg caattttctg catgtagagt accattttga
     4921 cgttctataa tagactttga tagagcaagc cctataccaa tactgtcctt actagcattt
     4981 ttaccacgat aaaatctatt aaaaatatat ggaagttctt cctgagcaaa ccccttacca
     5041 ttatcttcta tgataatctc aacatataga ggattttgac tataataagc aactatatat
     5101 ccaccatctt ctatatgctc actacaattt ttaagaatat ttaaaagagc ttctaaagtc
     5161 caattaatat cgatttcaaa ttctaccgaa ggagaagcct cgatagtaaa tgtctgatgt
     5221 ttttctctta aagaaccttc tataggctca attgcataag taagaagaga atgtacatct
     5281 ataagtttgc tttcaaatat taaagtactt gcatccaatt ttgataatgt aagtaaagat
     5341 gaaactagtc tctcaagtct tgagatttga tttgtaagtc tgttaatata atctatttct
     5401 tcatctggac aattttctga aagtaattgt gccataagtg acatagaggt aattggagtc
     5461 ttaagttgat gagcaatatc tgcaaggtta tctgaaagag tttgtctttc ttttaaagca
     5521 aattcttttg aacaacgaag ttccccaact gttttatata tctcatcttc aaggtgagaa
     5581 aattcatctt cacatcgtaa aagtacagac tcttttccta aattcacttc ttctaagtat
     5641 tgagtaagat tgtttattct actcagtttt tgcttatttg ttctatgttt gattatatat
     5701 acaactgtag ccattgttga aattatacca aacgatatca ctagaaaata aaaaaagttt
     5761 ttactccaaa atgtactttt agaatatcca tgttttgata aaatttcatt tcctgtttta
     5821 aaatccttat cactagaatt tttcagtgat tttagtatac tttctgtaga cttaggattg
     5881 tactcatatg atgcaccaag agccttagac agtacattgt attcattaaa agaagttata
     5941 gttgttagaa ttacactcaa taaaaaagtt acaattaagc agaatattat aaaaacaact
     6001 gtactgttga tatttcttgg agttttcact agttgtcctc cattctatat cctatgcctc
     6061 ttaatgtttt tatacaaggt ggatttttta atttttctct aagacgtttt attgtaacag
     6121 taagagtgtt atcatttacg aaattaccat ctacatccca gagcatttct agtaactttg
     6181 tacgagtcat agctctattt ttattttcca taagacttag aagaagttta tattctcctg
     6241 ctgtcaaagg tacttcaaca ttatctaaat atgctttgac ttcaacctta ttcaagctta
     6301 tattttcaca tgtaatagta ttgttagtat ttgaatgagt tcttctaaga actgcattaa
     6361 ttctggattg aagtactgag agcaaaaacg gtttaaccac atagtcatct gcacccatat
     6421 ctaatccacg tataatgtct ttttcatcat cacggactgt caaaaatatc actggagtat
     6481 cttctatact tttaacatat tggcaaaact cgtatccaat accatcaggt agatttaaat
     6541 caagcagaat taaatcaata gttttatcaa aatttttttt agcttcagca atcgtattat
     6601 aacaacatac agtgtgattt ttcttttcaa gggaagtttt tattccaaat gtaattgtat
     6661 cattatcttc taaaagaagt attttagcca tttactttta atcctttcag taatttagtt
     6721 attccattaa ctaagttatt agttttatgt ttaatatttt tcatattaga gatagaaaaa
     6781 attatcattt aagtatataa actttaattt ataaacttag gtttatgctt taattgaatc
     6841 tattaaaaga gcaagagttg tttcaataaa tttttcctca tctatattaa agttgttcag
     6901 gtatgatttt cctgttacag atagtaaatt aaaaaatcct gtaagtgtaa atattgatga
     6961 atacataaga gtaggtattt caatatcatg cctaatactt ctatcatttt ttccaatttc
     7021 aaacattcct tgtatttcat caaacataaa tttattaaaa gaaaaaaact tttctttaaa
     7081 cagcatatca gtatccgatt tttcagagtt tacaatacca atcattccca taaggcataa
     7141 tagagaaggt tctacagagt aaaatttatg aaaagcaaag tatgaaagtt ttattttttc
     7201 aaaccctgtt ttgccttttg cactttctat tttaacatta tcccaaagtc ttttatatcc
     7261 tcttaagaca acagcaaaga ataaatcttc tttacatgaa aagtacttgt atatggtgcg
     7321 tttagtatat tcaacttctt ttgctaattc attcatagtt gtattatcaa atccattaag
     7381 acaaaataat ttttctgcct tatcaataat ctcattttct ctttgtattt tttctttatc
     7441 tctttttgat aattctgaca tataaattat tcctttctat tgataaaaaa gtatactgat
     7501 ggtatattta tgtaaatata atatttgtat acctatagta tactttaata aaaaataaaa
     7561 ttaatatcaa gtattataac tatttataac tttattaatt aattatatag gtaaaatacc
     7621 aaataagggg aatttattta tgaagataaa agaaaatgta ttaatgttag atagtactcg
     7681 tggctcaaat tgttttattg tgtttgataa agaaattatt cttatagaca caggtcttcc
     7741 ttttatgggg aaaaatataa ttaaagaatt aaaaacactt ggcattaaac ttacagatat
     7801 taagcatatt ttattaactc atcatgatgt tgaccatata tctaatatta aattattgaa
     7861 agaaaaaaca ggagcaaaag tatgggctca tgtagaggat atacctttta ttataggaga
     7921 gaaggataga cctgggttta agaagtttat tggaaaggca atttccagaa ggctcattaa
     7981 ggatgttgaa ccttatggag agaatatgca gattgggaat ataaaaatta tccatactcc
     8041 tggtcatact ccagggcatg tctgcatgat ttttgaagat gtattatttg caggtgattt
     8101 agtcaaaaat aagaatggaa atatagttcc ttatcctaat ccatggaatt gggattataa
     8161 aaagatgatg aaatctataa gagaattgga taatttaaaa tatgaatgga tatgtatgtc
     8221 tcatggaact ccttgtatta aataataaaa attaacatgg attaatagag gcgtagttgc
     8281 ttgtatgtat gataataatt tacagcttct ttttaatata tcatgtatat ttgtagctgt
     8341 aagcatattt ataacaatac ttacaatttt gtattttaac atctataata ataattttag
     8401 ctttgttaac tatgaaattt acttaaagaa gtaaatattc aagtagtagt aattattaaa
     8461 taagataaaa aataaatggc agatgaatta aagtaatttc taccatggaa aatacttttg
     8521 agttagaaaa aactctattt tagagtatga agagatgaag cttccgaaga aagcaatatc
     8581 taaagtacat gttcgaaagg ataagtactt tttggtgcct gcttttagca ggttattttt
     8641 attttaataa aaataaaatc aaatgattat tttatcctta ttatttaaaa ctcttcccta
     8701 gaaaaagcaa ctaatgtata ttaaaataat atttagggag tggaaaaaat gagattagaa
     8761 tcgttagtta accaaaatta tgataattta aatgaaaatg ataaaattat tattaaacaa
     8821 atattgattt gtcaaagaga ataccgtaat ttttcgtgtg aaaaaattgc aaatgaatgt
     8881 aatgtttcta gagctacttt attaaggctt tgtagaaaaa ttggattaga ttcattctct
     8941 gatttaaagt atttacttaa aaataatcaa ctagaatttg ttaataatga taataatttt
     9001 catgatgttt gcgagaatta tcgactatta atgaatgaat tacaaaaaat atcatttcaa
     9061 gatatttgta aaataatata tgatgcagaa acaatttata tttatgggac aggtaatgaa
     9121 cagaaaagtt tagctcaaga aataaagaga atatttcttt cagtaggtaa atgtgttatt
     9181 gacttatttg atataggtga aattgaattt atgagagaat catttcaaaa aagtgattta
     9241 tttattatca tttctttgtc tggagaaaca aaagcaggaa ttgaaattgc aaaatttatc
     9301 aacaatcata tacatacgat ttctttaact aggcttgata ataatacaat tgcaacactt
     9361 tgtgagtata atctgtatgt tgaaactcaa aaacttgata cattacaagc tatatcttat
     9421 gaagtcgtag gagcatttta tgctctatta gatttgcttc atatgaatta catagagtat
     9481 agaaggagaa aagaatgaaa attgaaggac ttttcaataa atattataat tcatttacag
     9541 caaatgataa gtatatctgt gagtgtattc ttaatcataa agaagattgt attaaacttt
     9601 ctatagatga atttgcagac aaataccata tatctacaag tgcactttct cgttttgctc
     9661 aaaaactaca attacctgga tatagtgaat taagagttat tatacgctta aatgaaacag
     9721 aagtagaata taaaaatcaa agtaaacacc aattaatgga ttgctataat aaggtgataa
     9781 atgatataga gaagaaggat tgttcgatta tgtttgaaag aatgaaaaag gcaaatcgta
     9841 taatcgtctt tggagaaggt tattctcagg aaagagttgc taaagagatg aaaaggattt
     9901 tcttaccaac agggaaaatt atatatgatg tttatggtta tgatatgatt caggcactta
     9961 atcattttat aaaaacagat gatatgattt tctttatttc attgaatgga gatgcatcta
    10021 caattattga ttttgctaaa gaaatgagaa tgaaaggaat ttatatggtt tctattacaa
    10081 aaatgataag taatccttta gcaagattat gtgatgaaaa cctgtatatt caatctatat
    10141 ctatttctgt agataaacaa ctggactacg atgtaacaac accttatttt attttaattg
    10201 aactgcttta tattaaatac aagatgtatt tagaaagtga acatattccc cgaaaatgaa
    10261 aatttgtgct aaaaatgaac atatttttta aaagatatca aaatattgat atcttttttt
    10321 ttgaatgcta ttatgatttt gatagtaaga aaggaagatg aaattgtaca aattattaat
    10381 acttgatata gatggtacgc ttagagatga ggtatatgga atcccagaat ctgcaaagca
    10441 tgctatacgt ttatgtcaaa agaatcattg cagtgttgtt atttgtactg gaagaagtat
    10501 gggtacaata caagatgatg ttttatcatt aggagttgat ggatatattg caggaggagg
    10561 taattacatt caatatcatg gtgaattact ttataatcaa tcttttaatc aaagattgat
    10621 aaaggaagtt gtttgtttat taaaaaatag agaagttgct ttttcgattg agagtcagga
    10681 aaaagttttc atgaatcaaa aagccaaaga aatatttgaa acaatgaatc agttgaaagg
    10741 aactaattct tgtataaata aacaacatat ccaagaaaaa ataacatatg aaaataacat
    10801 tgaagagtat aagtcacaag atatacataa aatttgttta tggtcaaatg aaaaggtatt
    10861 tgacgaagtg aaagatatac tacaagataa aatggaatta gcacagagag atatttctag
    10921 tcagtattat gaaattattc aaaaagattt tcataagggc aaagcaatta agagattaca
    10981 agaaagatta ggagtaactc aaaaagaaac catatgtttt ggtgatggtc aaaatgacat
    11041 tgtcatgttt caagcatcag acgttacaat tgcaatgaaa aatagtcatc aacaacttaa
    11101 agatatagcg acatctatat gtgaggacat attcgataat ggaatttata aagaattaaa
    11161 aagaagaaat atcatataaa aaggagtaat ttatggaaaa gaaatggtgg caaaaagaaa
    11221 tcgtttatca aatttatcca agaagctttc aagatagtaa taatgatgga attggagatt
    11281 tacagggaat tatttctcgt ttagattatt taaaagattt aggtattaca tcattgtggt
    11341 tatgtccaat ttttaaatca cctatggatg ataatgggta tgatattagt gattatttgg
    11401 atattgcaag tgaatttgga agtatggaag atttaaaaga gttaattaaa aaaagtaaag
    11461 atagaggaat caaaattata ttagatttag ttcttaatca tacaagtgat gaacatcctt
    11521 ggtttcaaga agcattaaaa aatccagaaa gtccttatca tgattattat atttttaaaa
    11581 aaggaaacga tttgccaaat aactggaggt ctgtttttgg tggaagtgtt tgggaaaagg
    11641 tagagaatag agacgaatat tattttcatt cttttggaaa aaaacaacct gaccttaatt
    11701 gggaaaatcc aatattaaga agtgaacttt accaaatgat taatacctgg tctcaaatgg
    11761 gagtggcagg atttagagtt gatgctatta cattcatcaa aaagaattta agttttcaaa
    11821 gtttagaaag tgatggtgtt gatggtcttg ttaaatgtac aaaaacagtt agaaatcagc
    11881 ctggaataga aaaaatgctt tatgaattaa aagaaaatac gtttaaaaaa tatgattgta
    11941 tgactgttgc tgaagcagca ggtattagct atgaccagtt aggtgatttt attggagaag
    12001 atggtttctt ttcaatggtc tttgacttta aacatgctga cttagatgtt gcttctggta
    12061 gtgagtggtt taaaagaatt tcatggacaa ttccagattt aagaaaatgc attttagata
    12121 gtcaaatgta tgttcaggaa catggatggg cagctaattt tattgaaaat catgaccaac
    12181 caagagctac aacaaagtat cttttgcaat acgaaaggaa tgatgatgca gtaaaaatgt
    12241 tgggagcaat gtatttcttt ttaagaggaa ccccatttat ttatcaggga caggaattag
    12301 gaatgacaaa ttttaaaaga gataccataa atgattttaa tgatttatct agcattgacc
    12361 aatactatcg ttcaatagat gaaggttact cagaagaaga agcgttagaa tttgtgaatt
    12421 taagaagccg agataatgca agaacgccat ttccatgggc agataaagaa aatgcaggtt
    12481 tttcagatac aacaccatgg ttaaaggtta ttgaaaatta taaagatatt aatgcacaga
    12541 gtcagattaa caattcagag agtattttag aattttataa aaagatgata aagttgagac
    12601 aagatagtga gtattctgat tgtttgattt atggttcaat agaacctatt gatatagaaa
    12661 gtgatgatat tattgcgtat agaaggcaat tagatttggt agttattgat tgttattata
    12721 atttttctga taaacctgta gaaataagag tagaagaaaa atatgatatt gtattccaaa
    12781 atttagatga tataaatcta ttaaataaaa cagtgcgttt gaaaccattt catgcagtat
    12841 tattgaaagt gaggtagaaa aatgaataaa tataataaaa tagcaaatga gcttattaaa
    12901 attattggtg aagataatat tatttctatt acacattgtg ccacaagatt aagagtgatg
    12961 gttaaagata gagaaattat taatgataag aaagttgaaa aagttgatga ggttaaaggg
    13021 gttttcttta cttctggaca gtatcaaatt attttaggaa caggaatagt taataaagtt
    13081 tatgcagaag ttgaaaaaat gggattaaaa actttatcaa aaaaagaaca agatgaatta
    13141 gttaaaaata atgaaactgg atttaagaaa gttatgagaa ctctagcaga tatatttgtt
    13201 cctattattc cagttattgc agcaacagga ttattcttgg gattaaaggg ttgtttgttt
    13261 aatgataatg tacttggatt atttggaatg tcttctgcaa atattccatt gtatattcag
    13321 acacttgttt cggttcttac agaaacagca tttgcttttt taccagcaat tattgtatgg
    13381 tcagcattta aagtatttgg aggaacacct gttattggac ttgttattgg acttatgtta
    13441 gtttccccaa tacttcctaa tgcatattct gttgctgacc catcaaatga aattgaagca
    13501 attatggcat ttggatttat tcctattgta ggttgtcaag gaagtgtttt aacagctatt
    13561 gttacagcat ttattggagc taatcttgaa aaatggttcc gtaaacatat gcctaatgta
    13621 ttagacctta ttttcacacc attttttgtt atgctaataa caatgttagt tattttgtta
    13681 ggtgtaggcc ctataatgca tacaatagaa ttaaaaatgg ttgatatcat cagcttattg
    13741 attgatttgc ctttaggaat tggaggattt attattggat ttacatatcc attagctgtt
    13801 attactggct tacatcatac ttatgtaatg attgaaacat cactattagc aaatacagga
    13861 tttaatgccc ttattactct ttgtgcaatg tatggatttg cgaatattgg aacatgtcta
    13921 gcatttatga aaaaatctaa aaataatcaa gttaaacaaa ctgcagttgg agcaatgcta
    13981 tctcaacttt ttggtataag tgagcctgtt ttatttggga ttcagcttcg atataattta
    14041 aagcctttga ttattatgtg tgcatcttca ggtttaggag cagcaatatt atcaatttta
    14101 catattcaat ctaattcata tggattagca gttttacctt cttatttaat gtatatctat
    14161 gatggatata atttaatcac atacttatta gcatcaatat ttgtggttgc attctgcttt
    14221 attgtaacat gtttatttgg agtgcctaaa gaagctataa atgaagatga agacgaagaa
    14281 ttagtgttta atgaaaataa tgaaaacttt gtgtctccag ctaaggggaa aattgttgca
    14341 ttagaaaatg taccagatga aacattttct aaaaaaatgt taggtgatgg ttttgctatt
    14401 gatattatag atggaaaaat tgtttctcct atttcaggta aattagaaac agtggtttca
    14461 agtggacatg catttgggat taaaggaact aatggagaag tattaattca tgttggaatt
    14521 gatacagttg cattaaatgg agatggcttt gatattgctg ttaaacaagg agatatggtt
    14581 aaacagggag atgttcttgt taatgttgat ttaaagagaa tacatgaatt aggaaagtct
    14641 actttaacaa tggtattgtt tccagatggt aagaaggtaa atattttgga tataaacaag
    14701 gatgtaaaaa taggtcaaag aatatgtgtt gagtagtagg tgatatgtaa tgactacaaa
    14761 agaagattat caagagtaat tcaatctttt taaagtatga gaaataattc ataaactcta
    14821 aaacaattaa agaggttgtt tcaaaataga gattgtattg cacccaaatt gttgaaatga
    14881 tgaaaatata tttaacttca tacaaaaaat taaatagtgg caacaattga ggatgtgagg
    14941 gagagttgcc a
//
LOCUS       DAMJYX010000285        12096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.guided.101, whole genome shotgun sequence.
ACCESSION   DAMJYX010000285 DAMJYX010000000
VERSION     DAMJYX010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.guided.101"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            158..1564
                     /locus_tag="PC479_003706"
     CDS             158..1564
                     /locus_tag="PC479_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2806174.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1592..2440
                     /locus_tag="PC479_003707"
     CDS             1592..2440
                     /locus_tag="PC479_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2806175.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3101..3793
                     /locus_tag="PC479_003708"
     CDS             3101..3793
                     /locus_tag="PC479_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2806176.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3790..4725
                     /locus_tag="PC479_003709"
     CDS             3790..4725
                     /locus_tag="PC479_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2806177.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            4873..5796
                     /locus_tag="PC479_003710"
     CDS             4873..5796
                     /locus_tag="PC479_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2806178.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5780..6550
                     /locus_tag="PC479_003711"
     CDS             5780..6550
                     /locus_tag="PC479_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806179.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6560..7342
                     /locus_tag="PC479_003712"
     CDS             6560..7342
                     /locus_tag="PC479_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2806180.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7719..8255
                     /locus_tag="PC479_003713"
     CDS             7719..8255
                     /locus_tag="PC479_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2806181.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8287..8676
                     /locus_tag="PC479_003714"
     CDS             8287..8676
                     /locus_tag="PC479_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2806182.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8713..9099
                     /locus_tag="PC479_003715"
     CDS             8713..9099
                     /locus_tag="PC479_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2806183.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9226..10668
                     /locus_tag="PC479_003716"
     CDS             9226..10668
                     /locus_tag="PC479_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2806184.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10965..11780
                     /locus_tag="PC479_003717"
     CDS             10965..11780
                     /locus_tag="PC479_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2806185.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4359 a         1495 c         2280 g         3962 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taatcaatat aaaaaggaga aatatttatg aatacaggat ttccaaaaga
      181 ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg ataaagacaa
      241 taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac aggcagatac
      301 taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa tgaaagaatt
      361 agggatgaaa acatatcgtt tttcaatttc atgggcaaga ataataccag atggtgaagg
      421 agagataaac cagaaaggtt tagaatttta taataagata atagacaaac tgatagagtg
      481 tgatattgag ccattcgtaa ctttatacca ttttgacctt ccatttaaat tagtagagaa
      541 atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg ctaaaatatg
      601 ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac agaatttaat
      661 tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga agatagataa
      721 aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag tcaatgcatg
      781 tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg taacatatcc
      841 tcttacttct aagccagaag atgtatatgc agctagaatg aatgataatt ttaaagtgta
      901 ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga agtatcttga
      961 aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta aaaaagctaa
     1021 aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt tacctgaaga
     1081 ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt atggactatt
     1141 taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa tagacccatc
     1201 aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg ttataataac
     1261 agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg atgactatcg
     1321 tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag atggtgtaga
     1381 gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc aaggttttaa
     1441 taagcgttat ggacttgttt atataaatag aactgaccat gaagttttag atttaaaaag
     1501 aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg gaatagtaaa
     1561 ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat taaataattc
     1621 gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga aaggcattgg
     1681 ccataattat aaggccgggt ctgagttaaa taaaaaagat atagaaaaga tttttgttct
     1741 tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag atgcagaata
     1801 ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact taaaacttat
     1861 gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac gttttaaaga
     1921 aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc caaaagagta
     1981 tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag acctaccaga
     2041 agaagagatt ggtaatatag caggccattt tataaatgca caacaagata atccttatag
     2101 tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag ttcattatca
     2161 cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa tgcatctaaa
     2221 ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag attttatata
     2281 tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg taggagctta
     2341 tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt tgatgattca
     2401 tattcataag attttaggag aattatgcga aaatagttag aatatcttaa tatgctagta
     2461 atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca ttacttaagg
     2521 gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt ttttaaacct
     2581 ttatatcatt acaactttaa tgtttatacg actgtacttt agatgtatat ctacttttta
     2641 catttctcaa catgcaaacc taccattttt tgcagtatgt taattgaaat aaaccattga
     2701 gttaacccta aatttattct ggacttatag gaatagttcc aaagatactt taaaatattt
     2761 tctggaataa ttgcttattt cacatatgtt ttattgtgat gttttgattt aataaattct
     2821 tcaactctgt atatagtaaa tgtattatca tgtttgtttt attcaagcac acttataata
     2881 cgcttgattt tttcacaatg atgaaatata ctaatgggtg ttataggtat aagaatcgca
     2941 ttacacttga agaagtaagt gataaatgag gtataaatcc tcatatgata aactactatt
     3001 tcattgagga acatattcta agtgatgtca agatactaca atatgactaa attcctagtg
     3061 ataaaaaagt ctacgaatga gtgtagaaag gggaactaag atgaataaag ttttaattat
     3121 agatgatgat aaagatcttt gtattttgtt aaaaaagagt atttcaattg aacagattga
     3181 agcaaactat tgccattctg ggagagatgg actggttgaa ctttcaaaac aagattatca
     3241 gttagtagtt ctagatgtga tgatgccagg tatggatggt tttgaaacta tggaacaaat
     3301 acgaaaatat agcagtattc ccatcttaat gctgacatca aaaagcgata attacagcaa
     3361 agtgcatgga ttgcgagctg gtgcagatga ctatctaacc aaaccatttg aaattacaga
     3421 atttactgct cgtgtactgt ctttaatccg acgttacacc cgttttaatc caaaaaatga
     3481 ggagccacag caactttctt tcaaaggttt gttgattgac cttgaccagc atacagttac
     3541 taccaatggc gaacctatgt tgcttccacc caaggaattt ggtgtacttc tttactgtgc
     3601 aaggcatcag ggtaagattt tgacaaaaca gcagatttat gaagccgtgt ggggtgagcc
     3661 atatgtatat gatgacagca atatcatggc tattatcagt cgcctaagaa aaaaaattga
     3721 actagaccca tcatctccca tctatttgca aacaataaaa ggaattgggt atcaattcaa
     3781 cacggaggta tgaaaatgga tgaacaaacg ataattcttt tcttactctt gctgtcgatt
     3841 ggtctacttt ttctttccat atttgctttt tccaagcttg ggcatattta caagcgccta
     3901 aaagatatag aggagatact ggctgatgta caaattggag acgaaaatcg taaaatacta
     3961 attaagccat gtgatgttat ggctccattg gtttatcagc ttaatgagat tgtctacgat
     4021 tatgaaaata agttactttc tctgaaaaag tcggataaag ctagcaaaca acttatgaca
     4081 agtttatctc atgatgttcg tacaccatta acaactctga ttggttatct ggatgctgta
     4141 cacagtggta tagttatcga acaagagcgg gaggaatacc ttgaaatcgc taggcgtaga
     4201 gcctacgacc tcaaggatta cattgatgtt ctttttgact ggtttcggtt gaactctgat
     4261 gaatttacgc tgtcaataga atctgtggaa attgcagagt tgtcacgcaa tattttgaaa
     4321 gactggattc ctatattcca tgaaaagaaa ctgaattttg aaattgatat tccagaaaat
     4381 cgactaatgg taaacctcga cccagatgga tattcccgtg tggtcaataa tctggttcaa
     4441 aatgttcttg ctcacagtaa ggctcggcaa atcaagatta ccatgagtga ggattccaga
     4501 atggtattac tccgtgttga ggatgatggt gttgggattg ccagagaaaa tttaccacat
     4561 atttttgagc gactgtataa gtgtgataaa ggacgttcag aaaaaggaag tggtcttggt
     4621 ctatctattg tcagtcaaat ggtggagcga atgggtggtc aggtttccgt agaaagtgag
     4681 attggaaagt ataccgtatt tacagtgagc ttaccattaa tataaaattg tttttccccc
     4741 atgtgacatg tatacacatg gggatttttt gtaagttata aagttgcaag gttaatacaa
     4801 ggttgttgca aggttaatgt aagattgagg tggtatgata actttaataa acaaaggagg
     4861 cttttattat ttatgaataa tttaattatt gaaacaaaaa atctcactaa gcaatatggt
     4921 gctcaaaaga gcgtttctaa tttgaatatt cacgttcaga aaggtcgcat ttacggattg
     4981 ctaggaagaa acggtgcggg taagacgaca actatgaaaa tgttattggg tctaacaaaa
     5041 ccaactactg gagaaattaa aatatttggt caggacattc acaataacga gaagaagctt
     5101 cttcctcgaa ttggatgttt gattgaatca ccagggtttt atcctaatct gacaggaaca
     5161 gaaaatctca aaatctttgc aaggcttaga ggaataccaa aaaaaaatgc agtagaatca
     5221 gcactggatg tagtcggttt gccaaacaat gacaaaaagt tattttctca atactctctt
     5281 ggtatgaaac agaggttggc gattgctctt gccattatgc atgacccaca actgttgatt
     5341 ttggacgagc ctatcaatgg acttgatcct attggtattg cagaagtaag aacgtttatt
     5401 cgtgatctct gcacagaacg aggtaaaact attttgattt ccagtcacat tctttctgaa
     5461 attgccttgt tggcagatga cattggcatt attgaccatg gtattttgtt ggaggaagaa
     5521 agtcttgccc aattagaaga aaaaaacagt aaatacatac attttgctgt atctgatgca
     5581 gcacaggcag caaggcttct ggaaacccaa cttaacctga aaaatttcaa agtagcagat
     5641 aattacaata ttatgctata tgatacagat gtttctgttg cagaaataaa tcgtgctttc
     5701 attctaaaca acattgaggt atcagaatct catttatgcg aagatactct tgaagattac
     5761 ttcaagaaag taacaggggg tgaaggaatt gcttgatata atttcatgtg aattttcaaa
     5821 actcaagcgg ttaaaattta tactgatttc tattttaggt gcttgcttat ttccaattcc
     5881 cgcaacaatt ttgattgcaa aggacaactt gccctttgag caattgttca aattagttgt
     5941 caattttgga tattttttgc tattaccaat tgtattaagt attgttgcta gccaactatt
     6001 ttttatagaa agagataatg atgtattaaa aaatcttgca acagtgccag tgcaaaaagg
     6061 aaaactggct ctggcaaaat tggctgtgtt gctgtttata tcattgttct actctgttgc
     6121 agggttagga gctactatca ttggaggttt cattgttgga atagtagagg gggttgctgt
     6181 aaaattagga atgagcattg cttttggaat tatgtttttt gttactgtac ttcctgttgt
     6241 ggtactcatt gtatatttca atcgcagcta tattttttct attatacttt cttttgtata
     6301 tgctattttc aattttagta tatccttaaa tattatcaat tttgaaccaa ataacccaat
     6361 attaagtgta ttacctgctc ctgttataat gcgttggtgg atggcatttt ggggagatcc
     6421 cactggcgaa tacacggcac ttagacagcc atatttgctc tcaactcctg cttgtgtagg
     6481 tattttatgt ttgataacca ttatagcagt cctgctgatt tgtatgatat acaaaaagca
     6541 ggagaattag gaggtatcta tgcttgatat aattactaca gaatttcaaa aaataaaaag
     6601 atataatatt ctatggattg gtgttgttgc tgttctgttt tcagcactat tggcagtttt
     6661 tcaggttgta agttcacacg gcagtgaccc tctaacgtat gagagctttg caaacggagt
     6721 tatctggaat aatttcagtc ttagttttcc atttgggatt acacttattg gaggatatct
     6781 tattaaccgt gaatatacag accaaacctt aaaaaacatc ctaactgttc ctatttcatt
     6841 acgcaaattg ctagtgggaa aattaattgc ggttggagga attactgtat tgttaagtct
     6901 tttcagcttc ttatgcacat ttattttggg gttgttgttt tgcaatatca atatatctgt
     6961 tacactcgtt gtaaaatcct tagttcagat tttgtctgtt aatctgtatt gttatgttgc
     7021 tgttttgccg attattgctt attttggtcg taaacaaaat gcatttctga cgggtgtagg
     7081 gattgcattt gtatacggtt tttgtggtgt gtttgttgct ggaagaaatc tgacagactt
     7141 ttatccaatc acagcaggtc ttggtctagt tggatacaac aatggtgctg gtactgttta
     7201 tcagccactt atcggtgcta tgactttgat tgtaattcta atcttgacaa ctattctact
     7261 tgtatttaca cctaattatg ataaagttat gaccatatct aagaaaaaag ggataaagca
     7321 taaaaagtat tctcataaat aacataaata ttgtttctat tatttaagtt gctagacagt
     7381 gaaaagaaaa aatctgtcgt acagtgtcta tatgctcatg tattcaagaa gctacagttt
     7441 tgatttttca taactgtagc ttctttttgt atttattaaa tcacgactat cataatactt
     7501 gcaataggta tagcgacaag agagagtact gtcatatgtg ttttatatat tacattaagt
     7561 atattctaaa atatatgttt taaataatat atttgaaaat aggaatattt aaataaaaaa
     7621 aataatatta tatatagttg acaaaaattg gcaaatgaga taatatataa atatagaaat
     7681 agtaatcatt actattatta aaaagggagg aattaattat gaacttaaaa ggaactaaaa
     7741 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
     7801 actacgcttc aaaagcaaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
     7861 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
     7921 cttcaacaga ggaaaattta aaggatgcag cagcaggtga aaactatgaa tggactgata
     7981 tgtatgcaaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
     8041 ttgaagctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
     8101 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
     8161 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg cccagtatgt gaccatccaa
     8221 aagcgtattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
     8281 ttaactatga aattttctaa acaacgagaa ctgattttaa atgaaatatt aaataatccg
     8341 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatttaagc
     8401 ttaggaactg tgtatagaaa tttagctcag ctaacggaac atggctttat aagaaaggtt
     8461 agtattccag ggtatccaga tagatttgat ggtcgaatag ataatcacta tcatatcata
     8521 tgtgaagtat gtggagaggt atacgattta gaatccgaag ttcttaataa tttacaagaa
     8581 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
     8641 tgtaataatt gtaaaaggtg tagccaagta ggttagtttt caaattctaa aaataattaa
     8701 tgaggtgagt ttatgtgtag tgaacaaaaa ttttttatat gtgagacttg tggaaacctt
     8761 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggaa
     8821 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtaat tgaagtggat
     8881 ggaaataatg ttactgttaa agtaagtagc acaactcatc caatgactaa agaacatcat
     8941 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
     9001 ggagaacctg tagttaaatt tgctttgaat gatgatgata aagtgatttc tgcctatgct
     9061 tactgtaatt tacatggatt atggaaggct gaactgtaat atttaaataa attgagtcaa
     9121 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcaa
     9181 gttttaattt tgtttaatgt caatataata taaggagaaa ataatatgtc aatttataaa
     9241 tgtagtgttt gtggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
     9301 agtgaagatt gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
     9361 actgtttact atgaagaaga tgaaaagata gcagaagaca tagttgaaga tgaatctaaa
     9421 ttaaatactg aaaaagaggg agatttaaat tacttaagta catatttaag aagagatgat
     9481 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
     9541 gaacctatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
     9601 caattaaatc cattaccttt aaatgagcat gatgaagtaa atacaacaac aataatagga
     9661 aaaaaagcaa agaaacctat gattatagaa aatccagttt atatttccca catgtcattt
     9721 ggagctttgt ctaaagaact aaaaatagct ttagcaaagg gtgcagccca aaataagaca
     9781 gctatgtgta gtggtgaagg aggtatatta ccagaagaga aggaagcttc atacaagtat
     9841 atttttgagt atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
     9901 gctatagaga taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggc
     9961 gaaaaagtaa ctgaagaaat agcaaaagta agaaataagc ctgtaggtca agatgtaata
    10021 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    10081 cttagagaag tttcagaagg gcgtccaata ggtgttaaaa tttcagcagg tcatattgaa
    10141 aaagatatgg aatttatagc ttatgctaaa cctgattttg taactattga tggtcgtggt
    10201 ggagctactg gagcaagtcc aaagttgctt aaagatgcaa cttcaattcc aacaatattt
    10261 gctctttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    10321 acaggtgggc ttcgtatatc tactgatttt gctaaggcaa ttgctatggg tgctgatgca
    10381 gttgctattg caagttctgc tcttatggca gcggcgtgtc aacaatatcg tatatgtggt
    10441 tctggtaagt gtcctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    10501 atagaaaaca gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    10561 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    10621 actgttaact ctgaaatatc aaattataca aacatacagc atgtatagat tgaagtttga
    10681 aactattaga ttttagatag ctataaaagt aaatgataaa tatattttat aaaagagagt
    10741 gtcttaaaat gaacttttta gttcataagt cactcttttt tttatgaagc caaatatatt
    10801 tatcatttta gcaatgaaaa agaatcttat ctctttattt tgagatagac tccttctatt
    10861 tatgaaaatt aaagatatta aattgtaaaa tattctgtga tgtcgtacta ttaaactaaa
    10921 gattctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa agattgaaga
    10981 tacaagtttt agttgacaat aatacttata ttgatagata ctttgttgga gaaccagcag
    11041 taagctatta tattgagatt gatggaaata ggattttgtt tgactcaggg tattcagatg
    11101 tattcatatc aaacgctgaa aaattaaata tagatttagg aaatttaact catgttgttt
    11161 tttctcatgg gcataatgac catacaagag gtatccagtt tctagaaaat aggtatgatt
    11221 tatctacagt tgaacttatt tcacatccta actgttttat acctagaaaa catggagaga
    11281 aaagcatagg agctccattt tctgctgagg aaattaaaaa tatttttatg tataatccta
    11341 aggataagcc atttaatcta agtaagaatt gtgtgttttt aggtgaaata ccttctataa
    11401 atgattttga aaaaagagct aaaattggta aatgtaaaat aggagatttg tgggaagatg
    11461 attatgtttt agatgattct gctatagttt gtaaaactga taaaggtatt tttattgtta
    11521 caggatgttc tcatagtgga atttgtaata tagtagagta tgctaaaaag gtatgtggag
    11581 atgatagagt tattggtata ctaggtggtt ttcatctttt tgaattgaat aataggttag
    11641 atagtacaat acaatatttg gataaagaag aaatgagaat gttgtaccct tgtcactgtg
    11701 tttcttttaa ggtaaaagcg aagatgaatg aaatattgaa tataaatgaa gttggagtag
    11761 ggctgaccat aagtatatag tatttgaaag tatataagat ttaagttgaa ggttaaaaag
    11821 atattttcta tagaaaatat cttttttgtt acgtttattt taagagaaat caatatactt
    11881 ttatatgaaa tattgtaaaa aaatttattt ttaatgagaa tatattttca ttaataaaca
    11941 attttattat gtattaataa tgttagaata aatataaata aatcaaagtt acttacatta
    12001 cagtatattt tttcacaaaa tatattgatt tattctttta aagtttgata attccaatga
    12061 atgtaataaa agaaggaatt ttatactata cgatta
//
LOCUS       DAMJYX010000286        11166 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.guided.107, whole genome shotgun sequence.
ACCESSION   DAMJYX010000286 DAMJYX010000000
VERSION     DAMJYX010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.guided.107"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            172..510
                     /locus_tag="PC479_003718"
     CDS             172..510
                     /locus_tag="PC479_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806186.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            532..849
                     /locus_tag="PC479_003719"
     CDS             532..849
                     /locus_tag="PC479_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2806187.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            999..1676
                     /locus_tag="PC479_003720"
     CDS             999..1676
                     /locus_tag="PC479_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806188.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2004..2771)
                     /locus_tag="PC479_003721"
     CDS             complement(2004..2771)
                     /locus_tag="PC479_003721"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2806189.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3443..4258)
                     /locus_tag="PC479_003722"
                     /pseudo
     CDS             complement(3443..4258)
                     /locus_tag="PC479_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4359..5057
                     /locus_tag="PC479_003723"
     CDS             4359..5057
                     /locus_tag="PC479_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2806190.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            5145..5765
                     /locus_tag="PC479_003724"
     CDS             5145..5765
                     /locus_tag="PC479_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2806191.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            5921..6703
                     /locus_tag="PC479_003725"
     CDS             5921..6703
                     /locus_tag="PC479_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HDF2806192.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            6828..7754
                     /locus_tag="PC479_003726"
     CDS             6828..7754
                     /locus_tag="PC479_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2806193.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            7768..8217
                     /locus_tag="PC479_003727"
                     /pseudo
     CDS             7768..8217
                     /locus_tag="PC479_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8577..8984
                     /locus_tag="PC479_003728"
     CDS             8577..8984
                     /locus_tag="PC479_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2806194.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            8986..9216
                     /locus_tag="PC479_003729"
     CDS             8986..9216
                     /locus_tag="PC479_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2806195.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9606..9809
                     /locus_tag="PC479_003730"
     CDS             9606..9809
                     /locus_tag="PC479_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2806196.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            9887..10240
                     /locus_tag="PC479_003731"
     CDS             9887..10240
                     /locus_tag="PC479_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2806197.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10312..10944
                     /locus_tag="PC479_003732"
     CDS             10312..10944
                     /locus_tag="PC479_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2806198.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4119 a         1578 c         1745 g         3724 t
ORIGIN      
        1 aaaaatatga tttatccata tttgtttatt gtagatacag actatacaaa aataaatagc
       61 acttatttca gtataaaaac atatatagtc tatatttaga ctaaaaaagt ataaatagtc
      121 tatataaaac aacttaaact atgaatataa tctaaagtgg agggataata attggcaaat
      181 ataggaaaaa taataggaga tagaataaaa gtctatagaa caaaattggg atatagtcaa
      241 gagtttctag cagaaaaggc aggtttacat ccaacatata ttggtcagat tgaaagagct
      301 gagacaaata catctatcaa tattataatg aaaattgcta ctgctttaga cataccactt
      361 gaattgttat ttgcaaatat tattacaaca gaaggcgttg ataactctgt acctttagaa
      421 tgttatgaaa tgattaataa attaactgaa aaagaacaaa ttgcaatgct agagttaata
      481 aagtgcatca ttaaatacaa agaaatataa gtgagggaaa tagtaacaat aatgaattat
      541 ataggtaaaa gacttaaaga agaacggaaa aaagcaaatt taacttcaaa agaatttgca
      601 aatatggttg gagtatcgcc ttggtatata actcaaatcg aaagtggtaa aaagaatcca
      661 agtcttaaaa cattcataaa atttgtaaat atattaaata tttctgcaga tgttcttata
      721 aaagatatta cttccactgg aaaaacatat ttagaaaatg atataaatga agagttaaaa
      781 gacttaaatt caagagaact aaatctgata tcacagatta tagacatcat aaaaaagaat
      841 aaaatataat tctttatatt ttatataaaa aaaaaggtta tttgaaataa ataacctttt
      901 tttgtctttt tgtgttaaca ttgtcttata gtgctaatga gtactattag accatgttct
      961 atggccttat tttaatataa aacgattgga gataggctat gattgtgatt gaaggtagcg
     1021 ataaatttaa gattgcaaaa gaatatattg atgtagaata tactcttttt agcaaagtaa
     1081 cttttaggta tgaaaagttg aaatttaaag ataatgctga attggaaaaa attaagatgt
     1141 ttaaatataa aaatggctac atccctaata aattaaacct ttcttttgga tatggattct
     1201 cttcttataa aaagcaaata attagagaaa ctgtagatac tttaagattg acagaaattt
     1261 tttcaagcga gaacatagaa gatattaaat ttataaaaga tggtacaaaa aaattagaaa
     1321 ttagcataga gaaagttgtg aaatttaaac gtcgaaaaaa aagaaattat gtttgttgct
     1381 attgccctga tatgtataga gacataaaac tcgacaaaga atctatcaat aagatataca
     1441 acagaaaaat aaaaatagaa agagaagtta atatttttga agatgaggat gttataataa
     1501 acaagaaagt attgaagttt ccaaagtctt ggacaaagaa tatgcaaaaa tattggttaa
     1561 gtgaaaataa gtatcccata cattctactg taattgatga tgatagatat aaatgttgta
     1621 atgtacaata tacaaaaaat agagtgataa tattatatta catatataac cattaatata
     1681 cataattaca aaaaaataat gacaaatata cataatacaa atatatcttc tactttagat
     1741 aaaacagtta ttatgaaaac ttactatcta taaataatag aaatgctagt ataccatcta
     1801 taatctaatg tctagtaggt tttaatggca tattttaaat atgccatagt tgatttataa
     1861 gattttctat atcactttca aaatacacac taaatagttg cttttcagat ataattgccc
     1921 aatttaaatt ttactatctg aaaagttttt tatatttaat ttatatcttc aaaaataccg
     1981 tctaatattt ctatataatt aatttaatta atactcttat gtaatagatt aagtactgaa
     2041 tttaactctt taaatgatat ttgccctggt gctgaaacac tttttgcagc tccaaaagtc
     2101 aatgcagaac caaatatttc accacacaat ctacttataa cacccattcc tgacattgac
     2161 attgttatta ttggtctatc tgcataaatt ttaaacattt catttgtagc ttcaagtagc
     2221 actaacacat ctttttcatt ttgcggcata acagctattt tgggtaaatc tgcgcccaat
     2281 tcctgcattc tacataaacg agatacaatc tcttcttttt ttggagtttt attaaaatca
     2341 tgattagata taattacttt cacttctttt ttatgagcaa aattaactac ttcatctatg
     2401 acctcatctc ccataaataa ctctacatct attaaatcta ctaaacctgt attagaaatt
     2461 tctttattca aagtagtata ataatctctt gatattaatt tttctccccc ttctactaca
     2521 cttctaaatg tgaataataa agggatatca tgtatataac ttcttaattc atagagaact
     2581 tccttaactt ctttaatatt ttctacattc tcaaaaaaat caactcgcca ctctataata
     2641 tccaaacatg catcttttaa ctctttagcc tcttttataa tatctttttt atttttccca
     2701 ataattggta cacaaatttt aggtctacct tctccaatag taatattttt tacttgaact
     2761 tttcttttca tattctcctc cataatcaga aattctatag gactttaaat tctattttct
     2821 gcacaattta attatacatt aaaaatacta tacattataa aaatactaat ttaaaaataa
     2881 tttttattct aattcttaaa acaatcccac atattttaga aaaggtactc tataatatac
     2941 aaacacttct tttttaccta aatttatttt aacatcctat aattttcaca atattcttta
     3001 aattttactc atataaacaa tattattctg gatgctaata gaattaatct ataaatcata
     3061 gtataattat gcagaaataa ctcgaatttc actacttatg aagagattga atattaatga
     3121 atctaaatca tttgcattac ttaagagttt tgactaaagt tgaacaccat acaaaagtag
     3181 ctactcaatt gtttataccc caaccaagct taagtaatgt tatatagatt ctcgaaaaag
     3241 aatttggtat ctttttattt gaaaaactta tattaaattt gattatcttt tcataagttg
     3301 gtaaatgtcg atatatcctg tatctacaag gagtatcggc attttctttt tggaagatac
     3361 tttgcataag ttggcattta accgtataag aatgccatca aaagtagtaa ataagtagta
     3421 aactctagtc atactactag gattataacc gttccatttc agctctcgaa gaactaaatg
     3481 aaccatgtgc ataatagcca agcgtcatag taatgttttt atgccccata atatattgca
     3541 atatattagg actcattcct ttgttagcca tatttgtaca gagtgtatgc ctaaaggtat
     3601 gtggcgtaat ctttggtagc ttgtcctcat gggtcttatt atacttcttg actaacgctc
     3661 ttaacatgct ttgataatga gttgcttgtt ttggtaatcc atcttgttta agaaatagga
     3721 agttctggta accatcaaca atgattggtt ctgctttagc gttcttacgc cgttccacga
     3781 tacgttcaaa tgattgataa gctctttcag tcattggaat ttctcgctta ccctgtgtgg
     3841 tctttggaac ttctacataa tagccaatct tagtatcact caacaattga tggtctacca
     3901 taatggcttt atttttcata tccaattcta aagtcaaacc gcataattca gaaatgcgta
     3961 gtcctgtttc taagaggata acaacatcat cataatattt acaataagtc ttatcgctcc
     4021 ccataaagga taataagccc atttcctgtt ctggtgttag agcgattttt ggttctgttg
     4081 tatcttccaa aacatcactt aactaaaaat taaatggatt ctttctaata cagtcgtctt
     4141 gaattgccat gaaaaacgac gctttcagtg agcgcttata attgtcaatg gatttataag
     4201 cacacccatc ttcactcatt cggatagccc agcttttagc gtctgataat ttaaccatat
     4261 caatgctttt tgcacctagt gggtcattga tagtatctat attttgattt ataatttata
     4321 cattttttta aaaacagact tttaactgta aagaatctat gtataaactt agctatgttg
     4381 tccgtgtatc tacaaagaat cttgacgagc taaaaaggcg ttgtgatgaa gtcaaagact
     4441 tttacgacga cttatcaatc aagctcgtgc gtccctttgg ggatatgttg ggattgcatg
     4501 gcgaatttat tcctgtcagc aaacgttaca tcaatgatta tattcaatat gtcacttcgg
     4561 attttttggc tggacttggt tttggagcga cacaaaccct tggcgaacat gaggatatct
     4621 atcttggcta caatctggac actggaaaga atgtctatct aaaacctgcc cttgctagtc
     4681 aaggggtaaa gggttctatt accaatgcgt tagcgtctgc gtttatcggt tcattgggtg
     4741 gtggtaaatt attcagtaac aatatgcttg tttactatgc ggtactattc ggcggacagg
     4801 cggtcatagt tgaccctaaa gcagaacgcg gcaaatggaa agaaacgcta ccagaaatag
     4861 cccatgagat aaacattgtc aatttaacca gtaatgatga aaacaaagga ttgcttgacc
     4921 cttacgtcat tttgagccgt aaaaaggatt ctgaaagttt agcgattgat atcctaacat
     4981 tccttactgg tatctccagt cgtgacagtg ataagttttt gcagaaaaat gacaactgga
     5041 aaataataat ggaatagttt ctatgtaaat atatttttac tatcttacct aactaattga
     5101 aagatgatat aatttaatca aagatttggt aaggagggat gaatatgcat ttaattgatt
     5161 ttatgaatga agatattaaa aagaagcttg ttaaaaaaac gtataaaaag aaagagacta
     5221 ttctttttgc tgagcaagaa aatgaatatg taattcttat gattgacggt attgcagaag
     5281 cattcatctt aaatgtgaaa ggaaacatct caacgattca tctttataga agtggtagtt
     5341 tttttggaga aatggaacaa tttaatgatg ggaaaaaacc cgttgaaatt gtcgctttca
     5401 cagattgtaa tgtatataaa cttcatagag atgatttttt aaactggtta aggggagatt
     5461 ttgaagctac aaaatttctg attcgagaaa tatctagtaa attagttgta aatgctgaat
     5521 taattgaaga attatcatct ttaagtgtta aagaacgact tctgaggtgt atatccttac
     5581 actattatag aagtgaatta aatctgctta ccaaaaaaca gcttgctagc gaagtaaacg
     5641 ctccaattag aagcattaat cgtgctataa atgaatgtgt acaagaacat ctaattgctt
     5701 atgaaaacaa aagatttagt gtaattaatc agcgagaggt attaaagaat cttcctacaa
     5761 cataagtata atttcaaaaa agtattgaat aataattatt caatgctttt ttatttcggg
     5821 tcaagtgacc tatttttttt tatttcttca ttatataata ctcctatgca taattatgtt
     5881 ggattgatag ctaataaaag acagtgagag gtgttatatt atgactaaaa cacaacagaa
     5941 aatgatacaa aacgtattag aattttcaaa aagagatagt aggataatag gacttgcaat
     6001 tagcagttct tatatcacaa aatcgttaga tgaatattct gatttggatt ttctgattgt
     6061 agcagatgat aaatcctatg aagaagttat cgaagagaga ttacttattg tggaacagtt
     6121 tggaacatta gtttctgctt ttactggcga acacgttgga gaacctcgat tgataatttc
     6181 cctctatgat tcccctgttc ttcatgttga ttttaagttt acaaccttag atggtttact
     6241 agatagggtt gaagattcag ctattctcta tgaagaaaac aactgcataa cagaaattta
     6301 ttctaaaaaa gaagcatact tcccaactcc agacttacag tggattgaag atagattttg
     6361 gatttgggtt cattacgctt gcactaagat tggacgtaga gaactatttg aaacaattga
     6421 ttttttatct tttttaagac aatcagtact tgccccatta atacagcttc tcttaggtaa
     6481 attacctcgt ggagttagaa aaattgaatt ggacgcaccc caatatattg aagcattaga
     6541 aaagacggtt gctaaccatg aatataaaag ctgtgttaat gcattgcagg ctactatcaa
     6601 cctgtatctt gagttaagag aggaattatc acaaagtcct attgtccgaa aggttgaagc
     6661 tgaaagggtt gcattacagt attttcaaga tttaatcaaa taaacccaaa gtaaaaaaat
     6721 attgaatagt aaatattcag tgttttttca tttttggtca aatgacctag attagttgtt
     6781 gtaacaattc tataattatt ttgaaagtaa cagtatggag gataactatg agtgatattt
     6841 taagtagaat tacaaataaa attggggata agaatatagt tgataagtta tcagctctat
     6901 caaaatcaga tttgaactca cttctattag aagtgtttga tagacaagca aatactttaa
     6961 ctgcaactga tattctaaag tcataccaac taaatagatt tactatccct agcggtatag
     7021 atccagaaga aattcatgct ttggaatcaa aattattgag aaaagcattc aatatggata
     7081 tcaagacgat tatgctttct ccctctgccc cattaggtag ctgtagcgtt tttggctccg
     7141 tagatcaata taacgtagta agtgctctac gagggacaga aacacttgca gacccctcaa
     7201 atatgttggc aataattatt gctgataaat taaaaagaaa agaagaaaat aataccatac
     7261 ctatccacat ggctactact gctagggtaa taagagcaca aaattttgta ggtaaaggtt
     7321 tatattcaca ttttggctta tattgtatgg tttcatctgg tatagataca gggtcttaca
     7381 cttgtgaaaa aatgttgtta gaaaagcact taaactatta taaagatata ttaagtgaaa
     7441 tagaaaatgt tcgtttctct attattctac gcaaaagaaa cggatacaaa gataatgacg
     7501 gatttttcga cagaatggtt gaaactatca aacttatttt tcctaatata gagttatcaa
     7561 ttcataatga tgatgttgat aataattatt ataaaggtat caatttcaaa ttatcggtca
     7621 aacaaggaaa tgaaaccgtt gaaatagttg acggtggatt tgttgactgg acttatcaaa
     7681 tgcttggaaa taaaagagaa cgctgtttga taagtggtat tggcttagaa agatttcttg
     7741 tagctgtttc ataaaaggag aagaaaaatg gaacttatag agtggaatta tgcttatatt
     7801 accgatttag ttgagtttgc gaatgataaa gaaattgcaa agaatcttcg tgacaacttc
     7861 ccccaccctt atagggagca aaacgctaga gattttgtta gtttttgttt ggcaacttca
     7921 gatgttaaac aacttagcta tgctattttt tatcaagaaa gggctattgg aagtatcagc
     7981 ctaacatttg aagaagatac ctctattaat aaaaatgctg aattaggtta ctggcttgct
     8041 aggaactatt gaggcaaagg gatagcaaca aatgcagtaa aagaaatatg caaaattgcc
     8101 tttgaaaaat acaaccttaa caaaattcat gctaatgtat attcctacaa tagagagtca
     8161 agtaaagtat tggaaaaatg tggctttaag attgaagaat actgtcacaa ttcataataa
     8221 agaatagaaa catgattgat tgccatgtat atagtttaat tcgaccgcaa tagcatttat
     8281 tagaattaac ctacttttta ataacagaat aagtttttcc cctctattaa tttcttatgc
     8341 atctttgtat ttttatgaaa agctagccaa tgcatatttc catacatcaa tatggtggtg
     8401 gcttacaacc aatataaatt aaatacgttt taaagaatac acatttttgc cctataggca
     8461 gagtgtgtat ttttttctct tttttttgaa atttatcgaa gatttaacgc cctcccaccg
     8521 tgataggagt aagcaaggtt atttaactcc ttgcgatgga aagagggtga aatcttatgg
     8581 ttgatgaaaa gacaattgaa catcaattcg acagtttctg taaaacagtt ttgcgtaact
     8641 atgctagaga tatttatgac gaaaataaac gcagaaatga ttatttggta tctctcgaat
     8701 ctttgtcact tgcagaatta agtaaattga gtattttaga tgattatgat tctaactaca
     8761 tctgtatggt ttcttacgac tacaacatac gaattgaaga tgtattaatg gcgcaagcaa
     8821 tcggaaaact tacaaaaaga aaacaagata taattctact ttcattcttt ttcaacatga
     8881 caaatgctga catagcaaca ttgatggatt tagcagaaaa taccatacat tatcacaaaa
     8941 caaatgcact aaaagagttg aaagaactca tgcaggaaca ttgaaatgaa acctactaaa
     9001 gaagtattac cttttacaat tattttactt gctgtacatg gcgatgtaaa tgcaataaac
     9061 gtaatactta aacattttga acactatatc attcgtcttt cacagaaaac tttatttgat
     9121 gaatacggaa atccctatat tcatgtagaa gaagaaacaa agcgaatgct aggaacaaaa
     9181 ctaatcactg ctattttgag atttaatttg tcttaatctc gccctctctc catgtatcaa
     9241 gctatttggt acatggagaa tcactaatta tttgtctata aaagcccaca agctattgtg
     9301 attttttata ggcaaatcaa tgccgactgt tctttgacaa ctgaataaag tagatagata
     9361 cgtttgtgat acagatagcc aacaaatact atacgccatg accttaatca agagagctac
     9421 tatatagtga tttgtgaata acctttggaa cggttgtttt gccgtaacct gtaccacagt
     9481 ataatgatac acctgtatga cacggttctg tccataagaa tgggattggt tgaaatacca
     9541 atggagctta tcaaagccat ctatctactt ctgaactacg aacatagaaa ggagtgatac
     9601 attatatgaa caatactgat gtacctattt gggaaaaata taccttgacg attgaagaag
     9661 cgtcaaaata ctttcgtatt ggagagaaaa aattacgtaa acgtgctgat gaaaaccttg
     9721 attctggctg ggtcattatg aatagcaatc gcattcaaat aaagagaaaa caatttgaga
     9781 aaatcgttga tacacttgac gttatctaat gaaattgagc cttatatgtt ataatattca
     9841 tatgacgtat caagctcaat tcggttattg agaggagctt cttatcatgt caactgaaaa
     9901 aagacgagat aataaagata gaattttaag aactggagag agccagagaa aagatggaag
     9961 atacgcatat aagtatgtat attcatttgg taaaccacaa tttgtttact cttggaaact
    10021 tgtacctact gataaacccc caaagggaaa acgtgatgat atatcattaa gggataagga
    10081 aaaagcaata caaaatgacc ttgatgatag cattgacact attggtaaga aaatgacagt
    10141 ttgtcagtta tacgctaaaa agaatggact aagaaagaat atccgactta acactaaaaa
    10201 aggacgccat tatcttatga agattcttaa caaggagtga ttctgtgaag agatgtattg
    10261 tagtagtgga atagtcaaaa acataatata tattaaagga gagatttaaa tatgactatt
    10321 ccaagttcaa ataaggtata tccaagaagt aatgattatc aaactgtata tcttaaaaat
    10381 gtaataacaa gggaaaatat agaagttgga gattacacaa tatataatga cttttataat
    10441 gacccaagag attttgagaa aaacaatgta ttgtatcatt accctataaa caatgataaa
    10501 ttaataattg gtaagttttg ctcaatagca tgtaatgcaa aatttctaat gactagtgga
    10561 aatcactcaa tgaaatcaaa atccacatat acttttccca tcttttatga agaatggaaa
    10621 ctcgatatca accatataac ggacgcttgg gacaataagg gtaacattat aattggaaat
    10681 gatgtgtgga taggttatga ttctataatt atgtcaggtg taaatattgg tgatggtgca
    10741 attattggaa caagagcagt agttactaat gacgttccac cgtattctat tgttggagga
    10801 gtacctgcaa aaattataaa aaagagattt aataatgata caatttcaaa attattagaa
    10861 attaaatggt gggattggcc atataaaaaa attcaatcaa acattaagca tattcaatca
    10921 ggaaatattg ataaattagt ttaatatttt ataatttatt tatattaaac tttactttaa
    10981 taatcatttt ataagagctt tttcaaaatt tgtatattat tttaatatat ataaatttaa
    11041 tacaacatta aataaaataa aaagtaaagt tacttaataa aaagttactt tactttttat
    11101 tatttaaatt cctgttttta ttataatagg taaaattcta ttaaactagt tcacattttc
    11161 attatt
//
LOCUS       DAMJYX010000287        10299 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,13.7933590 isolate JH378
            SAMN32366194-rid16591143.guided.116, whole genome shotgun sequence.
ACCESSION   DAMJYX010000287 DAMJYX010000000
VERSION     DAMJYX010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366194
            Sequence Read Archive: SRR22862929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560785.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560785.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:28:40
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,739
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,614
            CDSs (with protein)               :: 3,614
            Genes (RNA)                       :: 84
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 41
            CDSs (without protein)            :: 41
            Pseudo Genes (ambiguous residues) :: 0 of 41
            Pseudo Genes (frameshifted)       :: 16 of 41
            Pseudo Genes (incomplete)         :: 18 of 41
            Pseudo Genes (internal stop)      :: 18 of 41
            Pseudo Genes (multiple problems)  :: 10 of 41
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366194-rid16591143.guided.116"
                     /strain="L,13.7933590"
                     /isolate="JH378"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Poland"
                     /collection_date="2012"
     gene            246..863
                     /locus_tag="PC479_003733"
     CDS             246..863
                     /locus_tag="PC479_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2806199.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="PC479_003734"
     CDS             971..3502
                     /locus_tag="PC479_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2806200.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="PC479_003735"
     CDS             3936..4637
                     /locus_tag="PC479_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2806201.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /locus_tag="PC479_003736"
     CDS             4687..5769
                     /locus_tag="PC479_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2806202.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="PC479_003737"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="PC479_003737"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2806203.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="PC479_003738"
     CDS             7127..7933
                     /locus_tag="PC479_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2806204.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="PC479_003739"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="PC479_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2806205.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT         3913 a         1077 c         1852 g         3457 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aacttatat
//
